LOCUS       DAOOXH010000001        78993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_268_length_78993_cov_7.667271, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000001 DAOOXH010000000
VERSION     DAOOXH010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 78993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 78993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78993
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_268_length_78993_cov_7.667271"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /gene="nadA"
                     /locus_tag="PKV64_00005"
     CDS             <1..742
                     /gene="nadA"
                     /locus_tag="PKV64_00005"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPN15495.1"
                     /translation="IVFCGVRFMAETAALLAPDKTVLLPDEGAGCPMADMLEAEDLVE
                     WKKQYPGRPVVLYVNTSAAAKAEADLCCTSANADAVVASLPEEEILFGPDKNLAAWTQ
                     RRTAKRIIAWDGWCHVHQGILPRDVAAARTRHPGAVLWAHPECRPEVLDLADRVLSTG
                     GMVREAAASAAADVIIATECGMLHRLRRDHPDKRFFPVKENALCPNMKKVTLPKIAAA
                     LETMAPRVTVPPEIAARARRAIERMIRL"
     gene            759..1451
                     /gene="nth"
                     /locus_tag="PKV64_00010"
     CDS             759..1451
                     /gene="nth"
                     /locus_tag="PKV64_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPN15496.1"
                     /translation="MTKAESSREKGKDGTPLPAADRLPEIIRILKREYPDPKPFLHFR
                     TPLEMLVATILSAQCTDKRVNLVTPGLFKKYPDARAYAEAGPGVLENDIRSTGFFRNK
                     ARNIRGAARKILDDFGGEVPATMDELLTLPGVARKTANIVLSNAFHKNEGIAVDVHVG
                     RVSRRLGLSGHEDPVKIERDLLRLVPRKHWGDFNFWVVSHGRAVCRARKPDCGACPLR
                     PGCPFPSSPRPR"
     gene            1548..2630
                     /gene="amrS"
                     /locus_tag="PKV64_00015"
     CDS             1548..2630
                     /gene="amrS"
                     /locus_tag="PKV64_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPN15497.1"
                     /translation="MVRRFSFDDETGDSTGVHKARLWSTAGDNAVRCALCAHRCEIHP
                     GHFGVCGVRENRAGRLVTRAYGEVVSAGADPIEKKPFFHFFPGSTAFSIAAAGCNFRC
                     RFCQNWQISQLRPIDGRASGGRRLAPGDIVRLAAANGCRSLSFTYTEPTIFYEYALDT
                     AVLGRAAGLMSTFVTNGYLTAEAVKVIRPFLDAANVDLKFFRDESYRKICGARLQPVC
                     DTIALMRRLGIWVEVTTLVVPGLNDGEAELRDIARFLADVDRDIPWHISRFHPDYEYR
                     EAVPTPIETLRRAADIGREKGLRFIYTGNISGEIEPTRCPRCRTTLIRRRGFEVMENR
                     LLNGRCPQCGEALPGVFPAAAVPSPL"
     gene            2718..4241
                     /locus_tag="PKV64_00020"
     CDS             2718..4241
                     /locus_tag="PKV64_00020"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPN15498.1"
                     /translation="MKIVLAGYNVDREALDEVLRAFPGRLDLTPETLSAAYARISRDP
                     RPADELRRAARREVEKARRSNAAIIFKMGHHSVAEHAVFNFDLMGISRLAIEEIEKFR
                     LCSFTEKSQRYIKLGDDYVVPEEIVRAGLGDLFVETVRAQNAAYHDLYRILRPLFFEN
                     HPALAADPKHHFLLDGWAKEDARYAVGLATEGQIGLTINARNLELMIRRLAAKPSAEI
                     RAIGLHLAGEAARVAPSIVRYTEAAAYDAETEEAFRDEAVESLGSRPSAAADRSRRSG
                     SVSSAVRMAGATPEGDERVLAALLHASWGGSYARCLREARRLPVQKKKALFLRVFERM
                     EFYDLPPRAFEHAGLTFELTVSASCYAQLKRHRMATLTAQAYDPGLGVTVPPSIEAAG
                     EAPRFRDVIGRTEAAYRILKEAAGPAAAYVLTNAHRRRVLLALNLRELTHLARLREDA
                     SAQWDIRRLAAEMSAAARARFPLAAMLLCGKDAYPDVYAKIFGRPPDKTPPSSLKNG"
     gene            complement(4287..5525)
                     /gene="tyrS"
                     /locus_tag="PKV64_00025"
     CDS             complement(4287..5525)
                     /gene="tyrS"
                     /locus_tag="PKV64_00025"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPN15499.1"
                     /translation="MTESIFNELKARGFIAQVSDEAAVRKMLDEKKTTFYIGFDGTAA
                     SLHAGSLVPIMAMIHLRRAGHRAIALIGGGTTMVGDPSGRTEMRQILDETTIRSFGES
                     IRAQLDRYLKFDGAGALAVNNADWLLPLKYIEFLRDIGRHFSVNRMLAAEAYKIRLER
                     GLSFIEFNYQLLQAYDYLTLFRTYGCTLQMGGDDQWGNILAGVDLVRRVEGAAVEALT
                     FPLLTTSAGAKMGKTAQGAVWLDGSLCPPYDYFQYWINTDDRDVGRFLRLYTLLPLGE
                     IQRLESLAGSELNEAKRILAYEATALTHGEDAARDARAAAAAAFGGSGQKDAASLPTT
                     AVARGRLEAGIAPAELFAEIGLAPSRGEAKRLIAQGGLSVNNEKIDSLDRKLTLADIG
                     PDGMILKAGKKKIHRVVADD"
     gene            5708..5992
                     /locus_tag="PKV64_00030"
     CDS             5708..5992
                     /locus_tag="PKV64_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010047812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPN15500.1"
                     /translation="MVKEEGTPDAPGPAWFLYILKCSDGSLYTGVTTDIDRRLMMHRE
                     GKASRYTRTRRPVELVYREECGSRSQALARECAVKSLPRAKKERLFDAPR"
     gene            5989..6240
                     /locus_tag="PKV64_00035"
     CDS             5989..6240
                     /locus_tag="PKV64_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15501.1"
                     /translation="MTPAARKARREKRAGPRRSLGRPPETGFPLKDLIARGVPVKVRR
                     FRPARGGFHKNQPYFKGVETSSLPESTAWVAGGDEVMMF"
     gene            complement(6288..6923)
                     /gene="purN"
                     /locus_tag="PKV64_00040"
     CDS             complement(6288..6923)
                     /gene="purN"
                     /locus_tag="PKV64_00040"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPN15502.1"
                     /translation="MSEEVFRSPKKHGRIMVLLSGRGSNFTAIADAAAAGKINAEIAL
                     VFSNKEDAPGLAAARGRGLPTMSLSPKAFADKAAYDRAILREVEARGIDLICLAGYMK
                     VLTPEFCAACKNRILNIHPALLPSFPGLNVQQQAIDWGVRYSGCTVHFVSAEVDMGAI
                     VLQAVVPVLQNDTAETLAARILVEEHKIYPEAARLYFEGRLEIRGRRVTIL"
     gene            complement(6920..7285)
                     /locus_tag="PKV64_00045"
     CDS             complement(6920..7285)
                     /locus_tag="PKV64_00045"
                     /inference="COORDINATES: protein motif:HMM:NF019278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPN15503.1"
                     /translation="MGTLYRVAKRYETPYSDPIRLARGARVTILPRTSEWPGWVWGRG
                     DDGKTGWVPDAYLRREGEEAFLTRDYDATELSVEAGDTIRPLGELAGWVWGTDDRGKT
                     GWVPKDHLEIDPPGGRRVP"
     gene            complement(7294..9522)
                     /gene="purL"
                     /locus_tag="PKV64_00050"
     CDS             complement(7294..9522)
                     /gene="purL"
                     /locus_tag="PKV64_00050"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HPN15504.1"
                     /translation="MKTRLHDDTVLARHNLTADEYGLIVKLVGREPNLTELGLFSAMW
                     SEHCSYKSSKVFLKTLPTRAPNVVQGPGENAGVIDIGEGRVIVFKIESHNHPSFIEPF
                     QGAATGVGGILRDIFTMGARPVALMDSLRFGPLDNPKNRSIMEGVVSGISSYGNSIGV
                     PTVGGEACFDEGYSLNPLVNVFCLGLAEKDKIFYARTEDAGNVLLYVGAKTGRDGIHG
                     ASMASQEFGGDTEAKRPNVQVGDPFKEKLLMEACLEVMERRIIVGIQDMGAAGLTCAA
                     TEMPAKSGKGAAIDLDRVPQREEGMTPYEILLSESQERMLIAARPDQVPAVKAAFAKW
                     DLDAVEIGAVTGDGRLTCRFHGDVVVDVPVSAVVDLCPAYKRPWKRPAAPEARAAGPV
                     PPPADYADVLRRLLSSPTIAGKEWIYGQYDHMVLTNTVLLPGAGAAVLRIRGSKKGLA
                     MTLDGNGRYAALDPRTGGKAAVAEACRNLACVGARPIGVTNCLNFGNPEKPGVMGQFK
                     ETVEGLSEACRAFGLAVTGGNVSFYNETGAAAVDPTPVLGIVGLIEDIDRLVTPGFKA
                     EGDAVILLGENKEEFGGSEYLKLIHGKRGAAPPAVDLEREIAVHETLLEAIGAGLVRS
                     AADPSEGGLAVCAAECAFHSPEGTGCDLSFETDLRPDAVLFGESQARILLTSRPENAR
                     RVLEIAEKRGVPARIVGTTGGRALRFRVSGRLLVDLPVAEARRLWMNAIPDFFAPAKR
                     PS"
     gene            complement(9519..10208)
                     /gene="purQ"
                     /locus_tag="PKV64_00055"
     CDS             complement(9519..10208)
                     /gene="purQ"
                     /locus_tag="PKV64_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HPN15505.1"
                     /translation="MKFGVVVFPGSNCDSDAFHACLDVLGQPTAYLWHKDHDLQGVDC
                     VILPGGFSYGDYLRSGAIARFSPLMREVRAFAAKGGLVLGICNGFQVLLELNLLPGAM
                     LRNKKLKFLCRQVTIRVENERTPFTRNARPGQILRIPIAHFDGNWYAPPETLKEVEDN
                     GQVVFRYCAPSGALTEEADVNGSLHAVAGLINKEGNVCGLMPHPERAVEPVLGSVDGL
                     VVLTSLLENRP"
     gene            complement(10219..10464)
                     /gene="purS"
                     /locus_tag="PKV64_00060"
     CDS             complement(10219..10464)
                     /gene="purS"
                     /locus_tag="PKV64_00060"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008032539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HPN15506.1"
                     /translation="MKARILVSYKDSVLDPQGQAVRNALHTLGYEAVRDVRQGKVFEI
                     ELRGVSRTEAERLLPEIAGKVLANVVIEKSSWEILED"
     gene            complement(10454..11755)
                     /gene="purB"
                     /locus_tag="PKV64_00065"
     CDS             complement(10454..11755)
                     /gene="purB"
                     /locus_tag="PKV64_00065"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPN15507.1"
                     /translation="MIERYTNPEMGAVWTEENRYRTWLAVEIAVCEAWNKLGKIPASS
                     LRTIKAKAAFDIKRIDEIERVVKHDIIAFLTAVNEKVGPDGRYIHLGLTSYDVVDTAL
                     SLLVRRSLLQVRARLLTLKKVLRKEALKHKKTVCMGRTHGVHAEPVTFGFKVLVWYEE
                     IKRHLDRIDRAVEVMSVGRISGAVGTYIHLDPRVEVLALKKLGLKPAPVSTQVLQRDR
                     HAEAIGTLALIAASLEKFTVEIRHLQRSEVLELEEPFTKGQKGSSSMPHKKNPVRCER
                     MAGLARLVRANVQTALENIPLWHERDISHSSAERIIFPDSFILTDFMLAEAIDIISHW
                     VVRKDRMKANINATRGLTFSQSVLLALTRKGLTREEAYALVQRNSLKTWEENLDFKAL
                     VLADPRIRERLSPAEIETCFSLDPYLEKIDYIFKRVLSRES"
     gene            complement(11821..12660)
                     /locus_tag="PKV64_00070"
     CDS             complement(11821..12660)
                     /locus_tag="PKV64_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HPN15508.1"
                     /translation="MAEIGKRNSLAIIKTVAFGVYLDGGGLGEILLPRRYVPAGAAPG
                     DRLDVFLYFDSEDRLIATTEKPLAQVGDFAFLEAVAVNPTGAFLNWGLPKDLLVPWSE
                     QRPPMKAGKKYVVHVYLDEASRRIAASAKIDKHLAKTPPPFEAGREVDLLIYGRTELG
                     YKAVIDNSHGGILYKNEVFRELRPGDRVKGYVRKIRDDGKIDLRLGKPGPEGVDELAG
                     RILDALRANDGFLEVTDKSPAEFVGRLFGVSKNAFKTAVGRLYKKRLVTLDDFGITLV
                     DRG"
     gene            complement(12733..13353)
                     /locus_tag="PKV64_00075"
     CDS             complement(12733..13353)
                     /locus_tag="PKV64_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HPN15509.1"
                     /translation="MKRKSVIRFLLTLGLFLPIIAAPLLRAHCDGMDGPVVKAAQKAL
                     ETGEVRLVLIWVHKQDEEAVIKAFEKTLSVRKLSEQAAELADMYFFETLVRLHRAGEG
                     APYTGLKPAGRDLGPAIPAADRAVESGSAQDLLNLLSDLVREGAGGQFKEVLERKAYE
                     ENDVEKGRAYVQAYVAFVHYVERIYEAAKSPAHAHFPDSESGEATQ"
     gene            complement(13368..13922)
                     /locus_tag="PKV64_00080"
     CDS             complement(13368..13922)
                     /locus_tag="PKV64_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HPN15510.1"
                     /translation="MKPTEDLKNEHAAVKLALKILEETARRLEEGVSVEEDHLGGLLE
                     FLRVFVDRCHHGKEEDLLFPAMERAGVPREGGPLGVMLAEHKEGRMYVKGMGAAFLEY
                     RKGGRTSGREYAQNARGYASLLARHIEKEDNVLYPLAESRLPAGVWKKLEAGFEKIED
                     EVVGAGRHEEFHAFLHRLKEEYLS"
     gene            14231..15130
                     /locus_tag="PKV64_00085"
     CDS             14231..15130
                     /locus_tag="PKV64_00085"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPN15511.1"
                     /translation="MTPSSRRRPSALLLIALIAGCLVLAVDCAPGPASVPADLVLKNG
                     VFYTVDASSSRAEAAAVKDGRFVYVGSDSGASAFIGKATKVVDLEGKLVLPGFIDSHA
                     HIISSFRRFFELNIFGRTTAEEVQKAIRDYATARPGAERISGRGWSNTAFPKTGPDRR
                     IIDAVVPDIPVCLASEDGHSRWVNSKAMALAGITKETKNPPGGFIERDPATGEPTGTL
                     RESAQDLVAGLFPSYTVDQLKQGIEAYQGMAAAFGLTTVHEASLDSWSSEIPASEIHA
                     ARVVLTLFRGKTVYAAGEGVERR"
     gene            15127..15636
                     /locus_tag="PKV64_00090"
     CDS             15127..15636
                     /locus_tag="PKV64_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HPN15512.1"
                     /translation="MNPRIKLLRGDITKIAAGAIVNAANGSLLGGGGVDGAIHRAAGP
                     GLLAECRTLGGCETGKAKLTKGYNLPAPYVIHAVGPVWRGGTGGEEALLAGAYRSALD
                     LAAENGIRSVAFPNISTGVYGYPKDKAAAVAIRTVSNFLDGHPEMDKVIFVCFDEDNH
                     RLYERRLGG"
     gene            complement(15839..17149)
                     /locus_tag="PKV64_00095"
     CDS             complement(15839..17149)
                     /locus_tag="PKV64_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPN15513.1"
                     /translation="MKKIFLLVVFVITALAVLVFSGPAGAEDGRTILIKNATLVPVTG
                     APIAAGQLLIRDGKIAALGTSVDAPAEALVIDAAGQFVYPGFIDAYTHFGLVEISAVS
                     GTVDVREMGKENPEVLAARAINPASIHFGTSRINGTTAALVAPAGGTFPGQSSLVRMD
                     GWTVAEMALKETATSLVNFPMSPRPAGGEGGGTAQESKVDVTSKLVEKISEYLKEARR
                     YLDLKKAAAADSRLEAPALNRKFEALAPVLDGTLPVILSVEKEKDIELALKFIKEEKL
                     KAVFRGCSQGFKVASKIKEAGIPVIIDSLYVGPVEPEDGYDAPFRNVIELVKAGVPIC
                     FSSGDDPAAGKDLPYHAARAVAYGLDHEEAIKALTINPARIFGVEDRLGSLETGKDAD
                     LFITTGDPLDTRSEVRHLFIGGRHIDMKNYWDGLYEKYQMRPIK"
     gene            complement(17160..18491)
                     /locus_tag="PKV64_00100"
     CDS             complement(17160..18491)
                     /locus_tag="PKV64_00100"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPN15514.1"
                     /translation="MKKKFFILALIGLLAAAAALPAAEERLLLKNAHVLPIAGENFDG
                     DILIIGTKIAKLGKDLPAEAGTEVIDLKGRWIMPGIIDSHSHIAIEGGVNETGDLITA
                     EVDIRDVLNPEDLNIFYALTGGVTSIHTTHGSANPIGGRGVCLKLRWGADAEGLLFKG
                     APLTSKWALGENPKQTNFSGAGKPRYPRSRMGVEESIRLEFDQARAYMKTWETYEKKI
                     KETRGRKGAAVPLPPRKDYRLEAVADILRGKLWVRCHAYQGPEMASIIRLCREYGVKL
                     AAFEHGLEGYRITNELVENNVAVSTFADFWAYKWEAFNTMPHSAALMAKRGVLVALNS
                     DDAERMRHLFLDAAKTVRFGGLSELEALKTITLNPAIIMGVDKLTGTLEAGKDADIAV
                     FNRHPLDPYTVCQMTIVDGKVYFDRAKYLDDRRKAEEAKKAAAEAPKEKKS"
     gene            complement(18508..20130)
                     /locus_tag="PKV64_00105"
     CDS             complement(18508..20130)
                     /locus_tag="PKV64_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPN15515.1"
                     /translation="MAKRRLPPASLFLCLGLWLASVPLLASEESLHEVWAVKDCRVVT
                     PGGPVLPKATVIIRGGLIESIGAETAIPPDAEVIDGSALTLHPGLIDGLGQSLLKLPE
                     EKFDPAKFYSGEFTDKDRGLTPELRAFDFAVLGKSTLEKYHKLGLTAVQAIPSKGVLS
                     GRASLFSLSSEDKNKALLLKDTWLGLGYAPASFMVYPNSLMGVVAFFRQEFADIGHYG
                     LNADLWAKMPAGIRRPVYNGRWDILREYASGKKPVVFFCNNQHDIRRSLALAEELKLD
                     YFLCDLGGEAQRVVPELKKAKARILCTVAFKAPATSRFSQLGKAEKEKAEKEFYPRNP
                     AALASAGIPFAFSSLGTDDPKSFMEGVQKAVEAGLSPDKALEALTSSPAAFLGLDKAL
                     GAVEAGRIANLVLVEGDPLAKEPKVKYVFADGRLFDFTKPKAKEGEKPTVNVSGRWEL
                     SLPEAGLTLTVDFAQEEASLSGKMTTPFGAFDFTGGSVSADQVYFEVNASVGGQDIDL
                     YFSATVTGDTMRGTAVQGTSGSTEFTGKRNPA"
     gene            20401..21129
                     /locus_tag="PKV64_00110"
     CDS             20401..21129
                     /locus_tag="PKV64_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPN15516.1"
                     /translation="MKKIFRIGAAAAGLLVYGAAGGHGFSDVFKTAEGDLKISLPGHA
                     TVFFEFGGTTVWVDPTKPYVFVPDHPPKADVILVSHEHGDHLDLEGIEAVKKTGTRII
                     CPAVCAGPLPGGEILANGGKTAVLGVSIEAVPAYNLAGMRSPGVPFHPKGAGNGYVLT
                     FGGLRVYVAGDTENVPEVKALKNIDVAVLPLMTPYTMTEEMLADAARAFKPDVLYVYH
                     TRTEDFSKLRNLLKDSGIDLRLPS"
     gene            complement(21177..21935)
                     /locus_tag="PKV64_00115"
     CDS             complement(21177..21935)
                     /locus_tag="PKV64_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15517.1"
                     /translation="MIVQIYEIQTPEEAERCLALGVDRLGSVILSETDRGRSRIREVV
                     ELVRGTDAKSSLIPLFSNENKLFSVMDILRPDYVHFCEALTDEQGRPHDLRPAAEKQH
                     RFRERFPGIGIVRTLPVPEAGRLPGFPALEIAAALEDSSDGFLIDTWLGREPVEGFVG
                     ITGRRPDLDRAAKIVRASRIPVILAGGLSPENVYESMRSVRPAGADSCTLTNALDREG
                     KPVRFKKDFDKVAAFVAEVRRAEKDFDGPPSARF"
     gene            22144..22216
                     /locus_tag="PKV64_00120"
     tRNA            22144..22216
                     /locus_tag="PKV64_00120"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22177..22179,aa:Thr,seq:tgt)
     gene            22254..22336
                     /locus_tag="PKV64_00125"
     tRNA            22254..22336
                     /locus_tag="PKV64_00125"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22288..22290,aa:Tyr,seq:gta)
     gene            22347..22419
                     /locus_tag="PKV64_00130"
     tRNA            22347..22419
                     /locus_tag="PKV64_00130"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22380..22382,aa:Gly,seq:tcc)
     gene            22441..22513
                     /locus_tag="PKV64_00135"
     tRNA            22441..22513
                     /locus_tag="PKV64_00135"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22474..22476,aa:Thr,seq:ggt)
     gene            22564..23766
                     /gene="tuf"
                     /locus_tag="PKV64_00140"
     CDS             22564..23766
                     /gene="tuf"
                     /locus_tag="PKV64_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPN15518.1"
                     /translation="MAKAKFERTKPHVNVGTIGHIDHGKTTLTAAITKVLATKGLAQA
                     KNYDDVAKADAKMFRRDATKILTVALAHVEYESEKRHYAHIDCPGHADYIKNMISGAA
                     QMDGSILVVSAADGPMPQTREHILLARQVMVPAIVVFLNKCDLVDDPEILELVELEVR
                     ELLTKYEFPGDTTPIIRGSATKAMEDIEGPGGKPIWDLIEAMDTFIPEPAREVDKPFL
                     MSVEDVFTITGRGTVVTGRVDRGTVKVGDEVEIVGFTETRKTVVTGVEMFRKLLDQAQ
                     AGDNIGCLLRGIGKTDVERGQVLAKPGSIKPHKRFKAEVVALTKEEGGRHTPFFTGYR
                     PQFFFRTTDVTGNAKLPEGVEMVMPGDNANLEIDLISEVAMEKGLRFAIREGGRTVGA
                     GTVTEILE"
     gene            23776..23925
                     /gene="rpmG"
                     /locus_tag="PKV64_00145"
     CDS             23776..23925
                     /gene="rpmG"
                     /locus_tag="PKV64_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPN15519.1"
                     /translation="MREIITLACTACKRRNYSNTRNKKKQTDRLEISKYCPFCRKHTS
                     HKETK"
     gene            23950..24026
                     /locus_tag="PKV64_00150"
     tRNA            23950..24026
                     /locus_tag="PKV64_00150"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23984..23986,aa:Trp,seq:cca)
     gene            24087..24299
                     /gene="secE"
                     /locus_tag="PKV64_00155"
     CDS             24087..24299
                     /gene="secE"
                     /locus_tag="PKV64_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPN15520.1"
                     /translation="MSDKPRWYKRFWAFLKEARAELKKVTWPSRAEVINTTIVVIIAT
                     VFFGFYLFFMDAFFAWALSKLKALIG"
     gene            24310..24840
                     /gene="nusG"
                     /locus_tag="PKV64_00160"
     CDS             24310..24840
                     /gene="nusG"
                     /locus_tag="PKV64_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPN15521.1"
                     /translation="MAKNWYVIHTYSGFEKFVSESIRQRAVETGIEDQIGQIIIPTED
                     VIEIKMGKKVVSTKRSYPGYILIEMEMTDENWLIIRDTPKVTGFVGSGKKPSPLSKSD
                     VSQIVTHMETTSEKPKPKHSFEKGEAVRIIDGPFFNFNGIVEDVNHERSTLKVLVTIF
                     GRSTPVELEFLQVEKL"
     gene            24851..25282
                     /gene="rplK"
                     /locus_tag="PKV64_00165"
     CDS             24851..25282
                     /gene="rplK"
                     /locus_tag="PKV64_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPN15522.1"
                     /translation="MSKEVVARIKLQITAKQASPASPVGPALGQHGVNIMQFVREFND
                     RTGKMEEGVVVPVLITVFKDKTFNFIMRTPPASYLLKKAAGIAKGSGAPNKDKVGQVT
                     QKQIEEIAKVKLPDLNANDVAAAVKIVEGTAKNMGLEIIRG"
     gene            25275..25967
                     /gene="rplA"
                     /locus_tag="PKV64_00170"
     CDS             25275..25967
                     /gene="rplA"
                     /locus_tag="PKV64_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPN15523.1"
                     /translation="MAKRGKKYLKARELRTRPEYSIDEAVKLLKQGGFAKFDESVDVA
                     MRLGVNPKQSDQMVRGTVVLPHGTGRSKKICVLALGEKVKEAEEAGADYAGSEELIEK
                     IAGGWIDFDVVIATPDIMRNVGKLGRTLGTKGLMPNPKSGTVTFDVKKAVLETKAGKV
                     EFRIDKTSIINASIGKISFPEEKLAENTRAFMAAVIHARPATAKGRFVRSMYLSSTMG
                     PSYKITEEILEK"
     gene            25978..26499
                     /gene="rplJ"
                     /locus_tag="PKV64_00175"
     CDS             25978..26499
                     /gene="rplJ"
                     /locus_tag="PKV64_00175"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPN15524.1"
                     /translation="MKKEVKAQRIDELKELFETNGTYYLFNYNKMTVAQATALRKNLR
                     KQGSALKIVKNRLALRSVPGPLADDLRPVFSKPTAVAYTGGDAVLLAKTLREFQVQNK
                     VLVVKGGVVEGTYIGSERFDEIVKLPGRMDLVAKVGGLMAAPMSGFLRCLRAPLGDFG
                     ILLNQLKAKKESA"
     gene            26530..26910
                     /gene="rplL"
                     /locus_tag="PKV64_00180"
     CDS             26530..26910
                     /gene="rplL"
                     /locus_tag="PKV64_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPN15525.1"
                     /translation="MSKLTKEEFFTHLDGLTVLELADYVKEFETRYGISAAAAVPVAA
                     AAPAAEAKAEEAKTEFAVVLKNAGEKKINVIKIVREITALGLKEAKDLVDGAPKTVKE
                     GLSKADAEAMKKKFEEVGAQVELQ"
     gene            26995..31281
                     /gene="rpoB"
                     /locus_tag="PKV64_00185"
     CDS             26995..31281
                     /gene="rpoB"
                     /locus_tag="PKV64_00185"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPN15526.1"
                     /translation="MIKESEHLYRERLDFSKIGQVFPMPDLLDIQVGSYKEFLQMDLL
                     PEERKDTGLQAAFKDVFPISDFKETTQLDFISYGIGNWECKCGRLKGVENSRAKCTSC
                     STLFPHGTVLTEKEICPFCNAARKIEVPVCEHCGDRVTLKMKYSPNECLQKGYTFSVP
                     LRLKVRLISWEKDATTKAKRLKHIKEQEVYFGDIPLMTDKGTFIFNGIERVVVSQLQR
                     SPGVFYRPGEVKGYFIAKIIPYRGAWVEFEYDVKNILYVRLDRKKKFLATVFLRALGF
                     GSDEELIKLFHAVVKITPENGKLYWEPVDSLAGRTAAEDVLHPKNGKVLVERKKKFTL
                     ENIAVLKEAGVPRVAVDRKDFQNAYALSGIKGKVKACEEIGDTALDLLIGKGEPFEIF
                     FPAEDAAGMIIVNTLKKDLRKDTNQALAEIYRKLRPGEPHTMESAANLFRSLFFNPQK
                     YNFSRIGRLKFNIKLGKETSLEEKVLQPRDYVDVISYLLNLRRGEGDVDDIDHLGNRR
                     VRSVGELVENAFRIGLTRMERTIKEKMTITLDLPSAMPQDLINSKPVIAALKEFFGSS
                     QLSQFMDQINSLSEVTHKRRLSALGPGGLSRERAGFEVRDIQSSHYGRICPVETPEGP
                     NIGLISSLSSYARVNEYGFIETPYRQVKDGRIIDYVKILHPGDSEYKYGDIVKKDDVG
                     KAVASLKRKGARHLPVVQPNCHYLTAWEEEDHNIAQANARVDEDGRLVDELVSARQAG
                     NFKLLPREQIDYIDVSPKQLVSLSAALVPFLEHDDANRALMGSNMQRQAVPLLRPVAP
                     LVGTGVEHLVAKGSGDVIVCKRSGTIAFVDAERILVHVDEANKTKDYEVATDLYVLTK
                     FLRTNQNTCFTQRPIVRKGDAVVKGQVLADSSCTDLGELALGRNIVAAFMPWRGYNFE
                     DAIVVSERLIKKDVFTSLHIVEETIEARDTKLGPEEITRDIPNVPENVLRNLDENGIV
                     RIGANVKAGDVLVGKVAPKGETQLSPEEKLLKAIFGEKALDVKDASLYCAPGVEGTII
                     DVRIFSRRGAEKGVRAKQIEKDEIARMKRNLDDECSILDSERERKVKALLKGTVVEKE
                     QKLGSLTIAKGQKLTERILDEIDLSDIGKIKLAADEDRDNRIKDVERKIRRQVEALKA
                     IFKEKVDALKKGDELAPGVIQAIKVYIAMKRKLSVGDKVSGRHGNKGIIARIVAEEDM
                     PRLPDGTPVDVVLNPLGVPSRMNVGQILEIHAGWAAQKLNLWLDTPVFDGAHEAEVKE
                     LLRKADLPESGKSILYDGLSGEPFDQEITVGNMYMMKLFHLVDDKIHARSTGPYSLIT
                     QQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEILTVKSDDVEGRAKIYEAIVKGDL
                     EFTPGLPESVNVLIRELQSLCLNVELGKDEKQEILQVLGIDATHILKGER"
     gene            31281..35435
                     /gene="rpoC"
                     /locus_tag="PKV64_00190"
     CDS             31281..35435
                     /gene="rpoC"
                     /locus_tag="PKV64_00190"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPN15527.1"
                     /translation="MDIKQTLGGKPRMDFDWVRISVASPEKIKSWSWGEVTKPETINY
                     RTFKPEKDGLFCAKIFGPINDYECLCGKYKRMKYRGIICERCGVEVTKSKVRRERMGH
                     IQLASPVAHIWFFKSPPSRIGLVVDLSIKDLEKVLYFESYIVLSGGDTPLKEKQLLNE
                     EEYKEAREKYGDKFEASIGAEAIKVLLERIDIKKEADRLRKAMKKETSQQRRLRYSKR
                     LRVFKALKKSGNRPEWMVLDVVPVIPPDLRPLVRLDGGRFATSDLNDLYRRVINRNNR
                     LKKLIELRAPELIIRNEKRMLQEAVDALFDNGKRGRVHLGANRRPLKSLSESLRGKQG
                     RFRQNLLGKRVDYSGRSVIVVGPELRLNQCGLPKKMALELFKPFIFHKLEKEGLVASI
                     KVAREWHEQERPEVWDFLEEVVREHPIFLNRAPTLHRLGIQAFDPILVEGKAIQIHPL
                     VCAAFNADFDGDQMAVHVPLSVEAQIEASTLMLSANNILSPANGRPLTIPSQDMVLGC
                     YYATMAKNGLRGEGRIFSNAHEVLLALEDKQISRQSLIKMRYTGPFMNLGVYYDDQAV
                     HTCPVTRIEKGLIETTAGRIIFNSVLPKSMPFFNGTFKKKGIESLVFYIYLRCGLRET
                     VETLDRLKELGFLQATQAGFSLGIDDFVVPKSKSELVEKANKKIREVEKLYLDGTISA
                     RERFNTIINVWSNVTDEVSGAMISEMKRISFETPNLNPLFVMADSGSRGNKQQIRQLA
                     GMRGLMSKPSGEILETPITSNLREGLNVIQYFISTHGARKGLADTALKTANSGYLTRK
                     LVDVSQDVIVDQHDCGTLKGVNVSAIVENGEIVEPFIDRVVGRTSLERVLHPDTGEVL
                     VDTNQEITETIAQEMQNLGIERVKIRSILTCEAKRGVCQLCYGRNMASGFTVELGEAV
                     GVVAAQSIGEPGTQLTMRTFHVGGIAMGEKEQSKIEAKNDGTLRFHGLRAVQGPEGHF
                     VVVNRTANIAVMDYRGREVEHYQVPYGAKLHLPDGGAVKARQLFAEWDPFNALILTEE
                     PGLVVFQDVVPKVTMEESQDEITGLMSQIIIEPKDEKLQPRIEIVDPQAKDKHGKPVL
                     LKRYFLPSGANLEVREGVAVEAGKILAKIPREQAKTKDITGGLPRAEELFEARRPKSP
                     AVISEIDGVVHFGGLVRGFRKLTVKSDRGVEKEYLIPKGAHISVGDGERIKAGLSLMA
                     GPVNPHDILRVLGEKELAEYLLREVQEVYRLQGVAINDKHIEVIIRQMLRWVKVEEVG
                     DTDFLIDEQVDKFMFQDENQKVIERGGKPAKARPLLLGITKSALSTDSFIAAASFQET
                     TRVLTEASIYGKTDRLRSLKENIIMGRLIPAGTGYKYYRDVELAEDEKPLEAEAPLEE
                     APVPET"
     gene            35575..35964
                     /gene="rpsL"
                     /locus_tag="PKV64_00195"
     CDS             35575..35964
                     /gene="rpsL"
                     /locus_tag="PKV64_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPN15528.1"
                     /translation="MPTVSQLIRKGRSMKGTKSKSPALERCPQKRGVCTRVFTTTPKK
                     PNSALRKVARVRLSNNIEVTGYIPGIGHNLQEHSIVLIRGGRVKDLPGVRYHIIRGTL
                     DSEGTANRFQSRSKYGAKRPKEKRAGS"
     gene            35980..36450
                     /gene="rpsG"
                     /locus_tag="PKV64_00200"
     CDS             35980..36450
                     /gene="rpsG"
                     /locus_tag="PKV64_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPN15529.1"
                     /translation="MPRRGIIKKRKPLGDQKYGSTLITKFITTVMQKGKRSTAENIVY
                     GAMDIVQEKTKEDPLKMFEKAMDNVRPMVETKSRRVGGATYQVPVEVELNRSLSVGAR
                     WIVKYAEERGGKTMKDKLASEILDALGNKGGAVKKREDTHKMAEANRAFAHYRW"
     gene            36517..38625
                     /gene="fusA"
                     /locus_tag="PKV64_00205"
     CDS             36517..38625
                     /gene="fusA"
                     /locus_tag="PKV64_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPN15530.1"
                     /translation="MTRTTPLADTRNIGILAHIDAGKTTTTERILFTTGITYKIGEVD
                     EGTAVMDWMAQEQERGITITSAATTCYWNHHRINIIDTPGHVDFTAEVERSLRVLDGA
                     VVILCGVGGVEPQSETVWRQADKYRVPRIAFINKMDRVGADPEHAVAMIRSRLGAYPL
                     PIQVPLGREDSFQGVADLVEMQVYDWGRQVTGMEFEKRTIGEAEEAEFRARRVEMIEA
                     LAEADEQLLEVYLSGAEIPPADIKAALRRATLAGRCVPVLYGASFRNKGVQPLLNAIV
                     DYLPSPADIPPVEGFHPKTHHREIRRPSDDEPFSALVFKLQNDPFLGALAYFRVYSGK
                     AKAGTAFYNSNKDDEERPGKFLEMHSNKRREVKELFTGDIAAMGTMKPVSTGDTFCAK
                     NRPVILEAIRFPEPVISAVLEPRTRAEHVKLQTALAKLTKEDPTLRVTQDPNTGQTLI
                     YGMGELHLEILTDRLSREFGLRVNMGRPQVAYKETILRAAEGEGVVDRPIAGKPQYAR
                     IRLAVEPLGRQAGFVFENRLKSGILPDVYAAPVEDGVREALDFGQLAGFPMTDLKVTL
                     KEADHREDETTEAAFKIAASAAFKTAAVKAVPVLLEPIARLEVIAPEEYLGDVVGDLN
                     ARRGAIDNVEARSGARVIHALAPLAEMFGYATVLRTLTQGRGVFTMEFHAYEAVPPQI
                     MEQIVARIEGRVSYDNEGRT"
     gene            38625..39827
                     /gene="tuf"
                     /locus_tag="PKV64_00210"
     CDS             38625..39827
                     /gene="tuf"
                     /locus_tag="PKV64_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPN15531.1"
                     /translation="MAKAKFERTKPHVNVGTIGHIDHGKTTLTAAITKVLATKGLAQA
                     KNYDDVAKADAKMFRRDATKILTVALAHVEYESEKRHYAHIDCPGHADYIKNMISGAA
                     QMDGSILVVSAADGPMPQTREHILLARQVMVPAIVVFLNKCDLVDDPEILELVELEVR
                     ELLTKYEFPGDTTPIIRGSATKAMEDIEGPGGKPIWDLIEAMDTFIPEPAREVDKPFL
                     MSVEDVFTITGRGTVVTGRVDRGTVKVGDEVEIVGFTETRKTVVTGVEMFRKLLDQAQ
                     AGDNIGCLLRGIGKTDVERGQVLAKPGSIKPHKRFKAEVVALTKEEGGRHTPFFTGYR
                     PQFFFRTTDVTGNAKLPEGVEMVMPGDNANLEIDLISEVAMEKGLRFAIREGGRTVGA
                     GTVTEILE"
     gene            39895..40212
                     /gene="rpsJ"
                     /locus_tag="PKV64_00215"
     CDS             39895..40212
                     /gene="rpsJ"
                     /locus_tag="PKV64_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPN15532.1"
                     /translation="MEKIRIRLKSFDHRLLDQSVKDIIIKAKRTGAQIAGPIPLPTRI
                     HRFCVLTSPHVDKKAREHFEMRVHKRLLDISEYNNETIEELQKLDLPAGIDVEIQAYG
                     AGE"
     gene            40216..40839
                     /gene="rplC"
                     /locus_tag="PKV64_00220"
     CDS             40216..40839
                     /gene="rplC"
                     /locus_tag="PKV64_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPN15533.1"
                     /translation="MRDGLIGKKIGMTQGYDAAGNSLPLTVLKAGPCTVIQRKTTKRD
                     GYEAIQLALIEDRPARKPGKALQGHYVKSGAPVMKVLREVRCDGETPLKEGDQIFVDV
                     FKAGEKVAVTGVSKGKGFAGVMKRHNFGGGAGAHGSMFHRAPGSIGASSYPSRVIKGL
                     RMAGRMGGERVTVRNLVVFEADKDNNILVVKGAVPGPNGGYVLIRKI"
     gene            40849..41469
                     /gene="rplD"
                     /locus_tag="PKV64_00225"
     CDS             40849..41469
                     /gene="rplD"
                     /locus_tag="PKV64_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPN15534.1"
                     /translation="MKKAKVIDSQGAVKAEIDLPESVFGYPAKGHLVYEAAVNYLANQ
                     RRGTASTKTRAEVKGSNRKPWKQKGTGRARAGATRNPLWRKGGTTFGPKPRDYSYSLS
                     KESKRNALRAALSAKQAAGRILILENLALSAPKTKEGASLLKALKVSSALVVDLGANK
                     NLFIALRNIPKVKPVEPSRVNTHDVVGHEWFVAGRDAFTALMERLQ"
     gene            41466..41762
                     /locus_tag="PKV64_00230"
     CDS             41466..41762
                     /locus_tag="PKV64_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPN15535.1"
                     /translation="MSKDPTQIILRPVITEKSTLLRERNREVCFAVARTANKSEIKKA
                     VEHLFKVKVDTVRTQIRPGKERRLGRSSGYRPDWKKAFVRLKEGEKMIEYFEGA"
     gene            41764..42591
                     /gene="rplB"
                     /locus_tag="PKV64_00235"
     CDS             41764..42591
                     /gene="rplB"
                     /locus_tag="PKV64_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPN15536.1"
                     /translation="MGIKSYRPISAGQRHKTGLAYEELTGDRPHKPLLESLPKKGGRD
                     NRGRLTMRHRGGGNKTMYRLIDFRRDKLDVPGVVETVEYDPNRSSFISLIKYRDGERR
                     YILTPLEMKVGQTIISSDRVVDVLPGNATLLRNIPLGTVIHNIEIHKDRGGQMCRTAG
                     SGAQILAKEGAFAQVRLPSSEIRKIHLDCRAVIGQVGNLDHQNISVGKAGRSRWMGWR
                     PHVRGTAMNPIDHPHGGGEGKAKGGRHPVSPTGIPTKGYKTRKNKRTQIFIVRRRSK"
     gene            42594..42884
                     /gene="rpsS"
                     /locus_tag="PKV64_00240"
     CDS             42594..42884
                     /gene="rpsS"
                     /locus_tag="PKV64_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPN15537.1"
                     /translation="MGRSLAKGPFIDSHLVEKIEVQAGQTKQRTVIRTWSRRSTVIPE
                     MVGMTIAVHNGRKFIPVFITENMVGHRLGEFSPTRTFKGHTTKAAKAVKVGA"
     gene            42886..43248
                     /gene="rplV"
                     /locus_tag="PKV64_00245"
     CDS             42886..43248
                     /gene="rplV"
                     /locus_tag="PKV64_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPN15538.1"
                     /translation="MNAPKYIASASTRHVRLSTQKARLVADLVRKRLVGEALTILRYN
                     AKAKPAQVLEKTLRSAIANAQQKNAGLDVDNLVIGKIVVNQGPAWKRIRPAPQGRAYR
                     IQKQTSHVHVYLEERTGR"
     gene            43252..43920
                     /gene="rpsC"
                     /locus_tag="PKV64_00250"
     CDS             43252..43920
                     /gene="rpsC"
                     /locus_tag="PKV64_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPN15539.1"
                     /translation="MGQKTHPHGFRLGFNKQWSSRWFAKGPEYARLIHEDLRMKQEIK
                     KKYAHAGISEVAVERVGPKIRVLIFTARPGIIIGRGGKEIESLKHHLESIVKREVYVD
                     IREVEKPELVALLVAEGIAVQLEKRIAYRRAMRKAVEMALKMGAKGIKVMCAGRLGGV
                     EIARSEWYLRGQLPLQTLKADIDYGFTEAFTTYGQIGIKVWIYRGDTEKAKMTSQTAE
                     VEEI"
     gene            43923..44345
                     /gene="rplP"
                     /locus_tag="PKV64_00255"
     CDS             43923..44345
                     /gene="rplP"
                     /locus_tag="PKV64_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPN15540.1"
                     /translation="MLMPKKVKYRKQSRGRMSGLADRGATLSFGQYGLQACECCWLTA
                     RQLEAGRITITRFMKRRGKLWVRSFPWKPVTKKPTEVRMGKGKGDPEFWVDVIKPGRV
                     VYEVEGVPEDVAREAMRLAAQKLPMKTRFVSRENRELR"
     gene            44342..44545
                     /gene="rpmC"
                     /locus_tag="PKV64_00260"
     CDS             44342..44545
                     /gene="rpmC"
                     /locus_tag="PKV64_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPN15541.1"
                     /translation="MKLSELRELSKDELLVKKAELKDQIFKLRFQHSLGQLESTAKLR
                     NVRREIARIETLAGEKDRKAKAE"
     gene            44555..44860
                     /gene="rpsQ"
                     /locus_tag="PKV64_00265"
     CDS             44555..44860
                     /gene="rpsQ"
                     /locus_tag="PKV64_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPN15542.1"
                     /translation="MNSKEQTPSRKTTKVGTVIGAKMAKTVSVQVERQYRHPLYKKIV
                     RTRRIFLAHDEHGKCKVGDVVRIVETRPLSRHKRWRVVEIVGLVPPSVETDLGEAQS"
     gene            44857..45225
                     /gene="rplN"
                     /locus_tag="PKV64_00270"
     CDS             44857..45225
                     /gene="rplN"
                     /locus_tag="PKV64_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPN15543.1"
                     /translation="MIQQYTVLKVADNSGARRLRTITPLGGGTGLVCSIGDIVSASVR
                     AADPESKVEKGKVVRAVVVRARKEIRRKDGSYIRFDDNAAVIIDKQGEPVGTRVFGPV
                     GRELREKKFMKIVSLAPEVI"
     gene            45225..45557
                     /gene="rplX"
                     /locus_tag="PKV64_00275"
     CDS             45225..45557
                     /gene="rplX"
                     /locus_tag="PKV64_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPN15544.1"
                     /translation="MSVPFIRKNDQVIVRQGKDKGKTGKVLSVDPDKGRALVEGVAMA
                     KHFIRPDRSKNVQGGIMEKESRIPLSRLMLFCPDCAEGVRIRSKKLQDGTRTRVCVKC
                     GTVLEPGK"
     gene            45565..46110
                     /gene="rplE"
                     /locus_tag="PKV64_00280"
     CDS             45565..46110
                     /gene="rplE"
                     /locus_tag="PKV64_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPN15545.1"
                     /translation="MSRLLERYKKEILAELEKELDIANPMAVPRLEKIVINVGVGEAI
                     QNAKALDAAKLELTQITGQAPVVSRAKKSISAFKLRKGLPIACFVTLRQRRMYEFFDR
                     LVNIVLPRVRDFRGVSPKAFDGRGNYTLGLRDQLVFPEIDYTKVERPRGMNITIVTTA
                     RTDAEAQALLKKLGMPFRETA"
     gene            46128..46313
                     /locus_tag="PKV64_00285"
     CDS             46128..46313
                     /locus_tag="PKV64_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPN15546.1"
                     /translation="MSTTSAYAKYLRTPKYAIRTRRRCKICGRSRGYYRKFEMCRLCF
                     RDLAHKGEIPGIVKASW"
     gene            46336..46734
                     /gene="rpsH"
                     /locus_tag="PKV64_00290"
     CDS             46336..46734
                     /gene="rpsH"
                     /locus_tag="PKV64_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPN15547.1"
                     /translation="MSATDPIADMLTRMRNAALMRRREVQIPASKLRTEIARILKEEG
                     FIRNYKVIKDGPQGILAVTLKYTEGNQSVLAGARRISKPGCRVYCTKDTIPKVYNGLG
                     LAVISTSKGILTGAKCEELGLGGEVLCTVW"
     gene            46760..47305
                     /gene="rplF"
                     /locus_tag="PKV64_00295"
     CDS             46760..47305
                     /gene="rplF"
                     /locus_tag="PKV64_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPN15548.1"
                     /translation="MSRIGKRSIILPAGVKAAVFADRIEIEGKKGKLTTPLYEGITAN
                     LEGTTLSFVRSDDTKQRRMFHGLCRALAQNAVIGVSAGFQKQLEIVGVGYKARVDRNH
                     LELSVGYSKPKLYPIPPDVEVVCEKPTLISVRGIDRQRVGQVADDIHRFRKPDPYKLK
                     GVRYVGEKLIKKERKAGVTGA"
     gene            47298..47672
                     /gene="rplR"
                     /locus_tag="PKV64_00300"
     CDS             47298..47672
                     /gene="rplR"
                     /locus_tag="PKV64_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPN15549.1"
                     /translation="MHKDKIKIRAERDERLRRRIRRKIQGTADRPRVFVFKSNRYIYT
                     QAVNDETGSVIASASTLEKDLRAALKNTKNLAGCEKLGEVLAQRLKARNIDKVVFDRG
                     VYPYHGRVKAVAEAMRKGGLAF"
     gene            47697..48236
                     /gene="rpsE"
                     /locus_tag="PKV64_00305"
     CDS             47697..48236
                     /gene="rpsE"
                     /locus_tag="PKV64_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPN15550.1"
                     /translation="MQTESTRKEGNRPPRPRLDDGTELKDQVITIRRVTKVVKGGKNL
                     SFAALVAIGDERGQIGIGKGKAREVPSAIAKAVEHAKKHMIRIQLADTTIPHMIRGEA
                     DAGVVILRPASKGTGVIAGGAVRIILELAGVKDILTKSLRSPNPISVAHATMDALMKL
                     KNPEQVARDRGKGKGPVEK"
     gene            48233..48430
                     /gene="rpmD"
                     /locus_tag="PKV64_00310"
     CDS             48233..48430
                     /gene="rpmD"
                     /locus_tag="PKV64_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPN15551.1"
                     /translation="MKAAASKKIKITLVRSLISRPESQRKVARGLGLGRLKSSVVRED
                     TPSIRGMIRKINHVVKVEGVE"
     gene            48427..48867
                     /gene="rplO"
                     /locus_tag="PKV64_00315"
     CDS             48427..48867
                     /gene="rplO"
                     /locus_tag="PKV64_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPN15552.1"
                     /translation="MNLSQLQPAKGARKKDRRVGRGPGSGRGKTSGRGHKGQLSGAGY
                     KAMRGFEGGQMPLVRRIPKRGFTNIFRHEPAVLNLDRLAKISGAEIGPAEMAAAGALK
                     PASARVKILGRGGIAEAKTIRAHSFSAAAVKKIEAAGGKAVVIE"
     gene            48872..50254
                     /gene="secY"
                     /locus_tag="PKV64_00320"
     CDS             48872..50254
                     /gene="secY"
                     /locus_tag="PKV64_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPN15553.1"
                     /translation="MLESIRNIFSIPDLRKRVFFTLILLAIYRIGGQIPNPGISASAL
                     AEFWNAQKGTILGFVDLFSGQYMSKMTIFALGIMPYISSSIILQLLTVVWPYLERLSK
                     EGELGRKKITQYTRYGTILICLVQSIGIAYFLQTLKTNTGQPVVPNPGLGFIFLTVLT
                     LTTGTVFVMWLGEQISERGIGNGISLIIFAGIVARFPAGLNSIISGLKSGDWNPIKII
                     LLTAMMVSVIAFIVFVERGQRRIPVSYAKRVVGRKVFGGQSTHLPLRVNTGGVIPIIF
                     AASIITIPATIAALIKTPAFQTFARWFNVGMPLYNLLYVAAIIFFTYFYVSIIFNPND
                     VAENLRKYGGFIPGIRPGKNTSDYIDEILSRITLVGAVYLALLAILPEFLTTGFKIQY
                     LPFVGRFLERSLPSWIMNGLGVNFYFGGTSILIVVGVAMDTLQQIEAQLVMRHYDGFM
                     RRGRIRGRRG"
     gene            50251..50901
                     /locus_tag="PKV64_00325"
     CDS             50251..50901
                     /locus_tag="PKV64_00325"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HPN15554.1"
                     /translation="MRVILLGAPGSGKGTLGERISRATGFPRISTGDLLRKAVREGTP
                     VGRKAEAVMKAGGLVDDATVTGLVRDRIAAPDCLGGYILDGFPRTVAQAEAVEAIDPA
                     RPEVVLDLLVSEDVIVARLDGRRICEACGDVYHIVNKPPKTPSVCDACGGDLVQRPDD
                     AAATVRERLRVYREATAPLQARYRAKGTYRPVDGDGFPEDVYSRAAGVLAGEGCRL"
     gene            50898..51689
                     /gene="map"
                     /locus_tag="PKV64_00330"
     CDS             50898..51689
                     /gene="map"
                     /locus_tag="PKV64_00330"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPN15555.1"
                     /translation="MIVYKTEAEIARMRESSRITGLILAELETLIAPGVRTQDLDAHA
                     DRRSRELGARPAFKGYRGFPGALCVSINEEIIHGFPSSRRLREGDIVSLDFGVFYDGY
                     YGDAARTFPVGRVEPDALRLIRIAEESFESGLLRFVEGNHLSDVSHAIQSAVEAAGFS
                     VTRSFVGHGIGQELHEDPQLPNFGFPGRGPRIRKGLVLAVEPMIAAGRWEVDILGDGW
                     TAVTRDRSLAAHFEETVALTDRGAEVLSRVGTKAVEPARKELPYA"
     gene            51682..51900
                     /gene="infA"
                     /locus_tag="PKV64_00335"
     CDS             51682..51900
                     /gene="infA"
                     /locus_tag="PKV64_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPN15556.1"
                     /translation="MPKEDVFEAEGVVRETLPNAMFRVELQNKHIILAHISGKMRKHF
                     IRILPGDRVLVEISPYDLTKGRITYRFK"
     gene            51913..52026
                     /gene="rpmJ"
                     /locus_tag="PKV64_00340"
     CDS             51913..52026
                     /gene="rpmJ"
                     /locus_tag="PKV64_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPN15557.1"
                     /translation="MKVRASVKKICRNCKVIRRKGLVRIICTNPRHKQRQG"
     gene            52038..52421
                     /gene="rpsM"
                     /locus_tag="PKV64_00345"
     CDS             52038..52421
                     /gene="rpsM"
                     /locus_tag="PKV64_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPN15558.1"
                     /translation="MARIAGITLPPDKRIEIGLTYIFGIGRSKSSQILQEAKIDKNKK
                     VKALSEEEVNKIRQIIEKREKIEGDLRKDISLSIKRLIDIGCYRGQRHKRKLPVRGQR
                     TKTNARTRKGPRGHTIKKLKPAAKS"
     gene            52437..52829
                     /gene="rpsK"
                     /locus_tag="PKV64_00350"
     CDS             52437..52829
                     /gene="rpsK"
                     /locus_tag="PKV64_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPN15559.1"
                     /translation="MATKAKTRTKKKVRKDIGYGTAHIQSTFNNTIITISDHAGNAVC
                     WTSSGTVGFKGARKGTPFAAQLAGKEAATKAREFGVRYLDVRVSGPGAGRESAIRSLQ
                     SGGVEIRSIKDVTPIPHNGCRVRRRRRV"
     gene            52841..53467
                     /gene="rpsD"
                     /locus_tag="PKV64_00355"
     CDS             52841..53467
                     /gene="rpsD"
                     /locus_tag="PKV64_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPN15560.1"
                     /translation="MSRYREPLCRLCRVEKTKLFLKGHKCLTDKCPVERRAYAPGQHG
                     RSRRRILGYGLQLREKQKMKRFYGMSEKQFRLFFERAERTKGITGEILLQMLERRLDN
                     LLYIAGYAHSRAHARQLVTHGHVLVNSGRVAIPSFMVKANDEIGFRPQSVKSEDLKAV
                     AEAYAGKTAPGWIQVNRENLTARVMALPTRADVTLPVEEHMVVELYSK"
     gene            53504..54529
                     /locus_tag="PKV64_00360"
     CDS             53504..54529
                     /locus_tag="PKV64_00360"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPN15561.1"
                     /translation="MTEFGFQRPKLLECDYETLTSTYGRFTAQPFERGYGTTVGNALR
                     RILLSSIKGAAITAVRIQGVLHEFSTIPGVVEDVTDIILNLKSIPLKLKGEGPSVLTL
                     RKEGPVTATSADLTGDGDVEVLDKTVAIASLDKDGLLDAELIVENNRGFITADQNFDE
                     TRSIDTIPLDSSHSPVLKVNYRVEPARVGKRIDYEKLTLEIWTTGAVAPVEALAQAAK
                     IMRDHLIIFLNVVDERADSEPVTAKREIPYAGQTELLAKPIEHLELSVRSNNCLRSAG
                     IEKIYELVMKSEEELLKTKNFGRKSLGEIKETLVTLGLGLNLDLNPKLLERIKGETKD
                     KAKETEE"
     gene            54526..54945
                     /gene="rplQ"
                     /locus_tag="PKV64_00365"
     CDS             54526..54945
                     /gene="rplQ"
                     /locus_tag="PKV64_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPN15562.1"
                     /translation="MRHRLSGRKLGRTTAHREMLLRNLATSLIEKERVRTTVAKAKEL
                     RPFVEKLITMGKTGTLAARRRALAVLMKEDAVTRLFETVAPRFSERAGGYTRIVKLSP
                     RKGDGAEMAVIELVGSEFKAKEKKKKDKEKGKKPAKA"
     gene            55320..>55685
                     /locus_tag="PKV64_00370"
     CDS             55320..>55685
                     /locus_tag="PKV64_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15563.1"
                     /translation="MADRIDLGIHPRTPYRVSAVLAAAAVIASSASFYFWGVFRRDAP
                     FIIGNMRGTALSMLVLAGPVLIVSMILTARGSLRARFVWLGSLAYIAYNALLFCFMVH
                     FNSFFLLFAALLGLSFWALL"
     assembly_gap    55686..55785
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <55786..56326
                     /locus_tag="PKV64_00375"
     CDS             <55786..56326
                     /locus_tag="PKV64_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15564.1"
                     /translation="SFFLLFAALLGLSFWALLTLLASFDLAAVQAAGSGVPGRTIAVY
                     MMICLIVFGFVWLQDIVPAMIHNTMPESFEGAGLAQNAIYVLDFAFTFPMLAVGAAWL
                     WRRRAWGYPISGMMVIMLTIETAGIAVDQAFGRLHDPSASAGAVPIMLALSGVGLIFS
                     VLFLRGINDRGSPTSRPAR"
     gene            56682..59183
                     /locus_tag="PKV64_00380"
     CDS             56682..59183
                     /locus_tag="PKV64_00380"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPN15565.1"
                     /translation="MRKSTVGLLLAGALVFFAVQASGETLMIRAKKIYTISGAVVENG
                     AVLIRDGKIAAVGTEIVPPPGTPVVQAWAVTPGLVDIHSHVGVYSVPSVEENSDGNEM
                     TNPLTPQVRALDSFNFEDPAIPVARAGGVTTIVALPGSGNVIGGTGVSVKLKDDRPDR
                     MVLQEDNCLKMAIEGNPIGVYGPRKQMPATLMAVYHLARKAFVEAREYEAAWEKFEKE
                     KKAGKAAAAPKRELGKETVLKALRREIPVHMHCATAAEAMSCIRLAREFNLRLTLAHC
                     YWVYLVVDELKEYKDVAFNVGPPMLFGYFDNPHVFHNTAAILAEAGLDVSLQTDALGG
                     GQQNLLQLAVLCVRRGMKEADALKAITLNGARAAGLDGRIGSLEPGKDADLVLYDGEP
                     LALTTRITQVIIDGRVEYRNESLKPLAGLTAAPLAARPLDLPENLGAAGAFAIRAGTV
                     FTLAGEPLRDAVILVKDGRIEKIGRDVAVPEGWPVIEAGDRVVMPGLVSPRSHAGISD
                     NWKRQSYVNETSQPVVPELEVKNSIEPQAPLFSFLRGDGITSLLVTPGNLNVIGGQGT
                     AIKSCGAVVDRMIIKDRAVMMFGLGANARREGEMPTTRMGIMALLRETLVKAREYGEK
                     RDRAARDKKNETPAADLALDALQPVLRGEMPVMIHAERRDDILAALRLADEFQLKIIL
                     DGATDAYKVVDEIRKRGIPVVLEGLFRGLGQVEDRGFNEDNAVILIRAGVTVAFRPDE
                     GSWRTPAAGQPGGEPLTLAAFAYRGGLTEEEALKAVTIEPARIAGLAKRVGSLEAGKD
                     ADIVILRGHPFLTGAIPEAVFIDGRLEARAQPDRR"
     gene            59203..60423
                     /locus_tag="PKV64_00385"
     CDS             59203..60423
                     /locus_tag="PKV64_00385"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPN15566.1"
                     /translation="MKKRNTVLWAAAVLAFLFVRPAGGGEADPLLAVRGGRIITVSGG
                     VIENGVLLIRGGRLEAVGRDVAVPPGAEVIEAAGAVITPGLIDAFTHLGMERPEAGGG
                     DYDESAKAVLPQLRVIDAIDWENEDFPAACSEGVTSALSAPGEGALLSGQSALVRLPG
                     HSSGGGETLRFPAAVHANLGEAAKLRFGSRNQYPSTRMGAMALLRQTLLDARQAMESK
                     TGPDRAAAAAVESLGPVLKGEMPLVVRANRLDDILTAVRLADEFGLRLVINHGAAAFR
                     AASELAERKIPVLVGPLADFRSTPESAADSLESAVRLQRAGVKIAFQGNSGRRPDALL
                     EIARLAVAHGLPEEEAWRALTLNAAEIFSVDGRIGSLEAGKDADVVIFSADPLRETAV
                     VRTVISRGRIVFSR"
     gene            60673..61500
                     /locus_tag="PKV64_00390"
     CDS             60673..61500
                     /locus_tag="PKV64_00390"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HPN15567.1"
                     /translation="MKRGLRVVPVVLCLCFSPAVRAAETVRMERMNWPDIKKAIESGC
                     TTAVVGVGSIEQHGPHLPTMTDARISDALAELVARKLGGALQARTIETGLSDHHLAFA
                     GTISLKPETLRLILRDYTASLVRHGFKRILFIPMHGGNFETVGRFAAEARKAHPGIDV
                     TAFLDLPGLTDFLSRLSAEYGVSKTEAGDHAGENETSMMMALEGGLVASERFAPGYCG
                     PFGGAEMRLLLEKGMPALSADGVLGDPRKASAEKGRVYLERLADFIVASVGGTSGRR"
     gene            61808..63079
                     /locus_tag="PKV64_00395"
     CDS             61808..63079
                     /locus_tag="PKV64_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HPN15568.1"
                     /translation="MKASALFSEKKPVLGINSLGRIGKLTLWHHVGRKYFKEIVVSQG
                     REIGAGMDAVVQTIEKDSTYGRMHTFLYGINAEPRIQIVDEKEGKLLIDGIPVTVLRK
                     DRNPKDIPWRDYGVDVVADCTGQFLDPTAEPDAAKGSLRGHLQGGARVVLASAAFKLK
                     NRALPMPEDAVTLIYGINHRAFDPDRHSLVSAASCTTTALAHMIKPLVDHEKTRAILT
                     ASASTVHAATNSQSVLDNVPKAGAKDLRKSRSVLDNIILTSTNAAEALALAVPEVKAI
                     GFMADSIRIPMKTESIIILNVTFQNPGDAGVPAIDARAINEIYRQAAARDPERRLVFS
                     EEQNVSADVAGVDASVVIEGQFNHTRTAFIDIDVSQISALPPETRAAFPVKRLEIPVV
                     HAKVFGWYDNEYGSYTNRMGDLAVHIQQSLK"
     gene            complement(63104..67303)
                     /locus_tag="PKV64_00400"
     CDS             complement(63104..67303)
                     /locus_tag="PKV64_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPN15569.1"
                     /translation="MSRTDAHSRRTGGLDPVLPPPARTLIELVRESYGTHLRIRNFLE
                     DYHRTGRKEAARAEDWRRISLEDFHVFDAHPRGREAHEIVVDLFFEMIEPSQEEDVRE
                     KSLAGLFEYLRKIMNDSGPNLDRNLPLVDRAFNRVLSAVPDGDSLLKKSARDMKLILG
                     TCLERNLPLSRGAYQSLAGRAFRSSFDYWLAQPDPEEGFIPFKAEDGDGADRDLYLSL
                     VEPLSRKRLREWKSLVDACDGGSEPGDWAGFSGLLEWPDYYQIAKGYLKIADEIEKAS
                     FSDGRRHEIRLLYLFRIISVPGFRDIHREALIEINRSLGRVFAGLPPDKREPMISLLF
                     QTFRESAARAAYPDTMMSCLSTAAREVFKLDRHPEVKVLLDEIIRFGFVGPGIRGATT
                     DWEVKFNPLHVKNIRAWIEIIALKPRWTKPLLSALIAHLKIGGVFVRDADLLQKDISA
                     LLNADITPAHNLVLQLARLFPVYFNDINAGGELRDNTTRLDALCFREDPLIHFIRKQT
                     HVECNSTLVPFCEAVLEYWAAGRKEGMKPFLPAEIYDALQPSGPFYDGLHRVMAGVSR
                     EAGGDLRETLAWDQMKILKTVREIPDVPEPDKEKAALFLRLYQLLHRKYFVQHQDVLK
                     DLDASFLFDRRKIDILKRALARQTPGPALAVLLEFLEILKDRFLSPKKTEALEDLYEK
                     RHIAVGIPSVYGTYREEKFDALRLYLKLQSLADVQLEHALAPVNLRFITRSTLVEIHR
                     FLVIFLKVLALEGIATEGLTSKVRLLEGALKAPRFSMAQYTDIFQFISKGVQDVIHVY
                     FIDAHRARLPLVIRQVLAALPQEERPSPDKEEEEIYIRTENFIRSLIASSFGLQTIDN
                     FVSTILRTLCAEEEKFKDQAHLRNVLMSYDPGAAVVPIGEKMGPRDDQILLGNKGYFL
                     KKLHSFGFPVPPGFILTTEVFRCKEAIRSDRNIGRDLDQRIDRQLRILEQTARKKLGD
                     PHNPLLLSVRSGSAISAPGMMTSFLNVGLNDQIAEALAARPRYAWAAWDCYRRFLQFW
                     GMSEGMDRDFFDDTIDQFKADLGIVKKFDFTPEQMRDIAFAYRKGLSQSGITLPDNPR
                     EQLDKAFFKVIESWDSPRAEIYRAEMNISSDWGTAVIVQAMVYGNLNSDSGTGVIFTR
                     EPKGLTSSVSLFGDFTHGAQGDDIVTGLVETYPISETQRISEGRDEPISLESQFPAVY
                     GELKRLAETLVYEKGFEHQEIEFTFESATKEGLYILQTRDLPPPEPARVKVFTRTEKL
                     EKSRLGNGIGISGGALCGRAVFSEADIEEFSRQDPNVPLILIRPDTVPDDIGLLRGVE
                     GLLTAKGGSTSHAAVIIPQLKKVGVVGLRSLKVDEKSGTGTIHDAVIRRGDFLSLDGW
                     SGAVYRGRHRIEPEPAVPAKV"
     gene            complement(67300..68859)
                     /locus_tag="PKV64_00405"
     CDS             complement(67300..68859)
                     /locus_tag="PKV64_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPN15570.1"
                     /translation="MEKPIEPDRVFLGGDLQPWKGRRLDVYSPIDVRTQEGFSPLRLG
                     SLPRMTGRDALRALAAARKAFCGGRGAWPSLTLAGRIRRLEDFSARLAGLKEPVVRTL
                     MWEIAKPRVELEAEFDRTIELIGRLIQAAVKRDRASRSLIRQKGIIGLVCDEPRGVAL
                     CAGPYNYPLFETFGLVVPALLAGNTVIIKPPRFGVLFFDFLLEPLASCFPPGTVHVLS
                     GDGPAVLDPLLKSGGIDVFAFIGSKAVADRLTSLHPKRTRLRVLLGLGAKNAAVILPD
                     ADLETAVGECLLGALAFNGQRCAALKILFVHEAVRKPFLDRLAAETAKIRIGMPWAKG
                     VRITPLADPVRMSYLRSLIDDAAGRGADIINENGGRSAGSLLCPAVLQPVTPRMRIYR
                     EEQFGPLVPIVDFRRMEEPVDYLRASSFGQQASVFGTDVRTLGPLIDAARAQVARVNL
                     NAKCQRGPDVFPFTGKKDSARGDFSVSEIFGFFADRTVVAARDDGRGAALLKRYRAVS
                     GRSGSGERNVS"
     gene            69270..70019
                     /locus_tag="PKV64_00410"
     CDS             69270..70019
                     /locus_tag="PKV64_00410"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPN15571.1"
                     /translation="MEDKTAYFFEVYGALPRAGPGDDASTEKAFRMMNGVPEKPMILD
                     IGCGPGKQTLVLARISGGRIIALDNHRPFLERVEADARALGLSARIKTVLQDMKTMDF
                     APGTFDVIWSEGALYQMGFAAGLRACRPLLKAGGSIGLTELVWLTDDRSSEAVKWAWD
                     YADMKDVKDNLALFEACGYEVDGHFTLPASSWIDSYYGPMRARIRELRETCRDKAAAR
                     EVLDAAQAEIEGFEKCSTELGYEFFVARRTE"
     gene            70058..70954
                     /locus_tag="PKV64_00415"
     CDS             70058..70954
                     /locus_tag="PKV64_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPN15572.1"
                     /translation="MKQRRMIRLVVNLGLAGNAVLAAAKLGLGILGHSKALLADGINS
                     VSDVVYFLVVKFFVGMSGKPADREHPYGHHQYETIAALVVGAFVVTSGLAIFWDAVDS
                     LFDLIKGKETPAAIASFTLWTAAGTVAAKLFLMFQARRAGRVTDSLAVKAIARDHRND
                     VLASLGVGAGILLSLSGHPWADPIAGAAVAVIVAKTGFSILLESADELMNTLPSEEMK
                     ERFAASLRDVPGVVSIEDIRAHRFGPYLVANIIIRVNSALRVGEADEIATAAETRLRE
                     DVEMLRKVYVHIHPAEGGRLSG"
     gene            complement(71097..71867)
                     /locus_tag="PKV64_00420"
     CDS             complement(71097..71867)
                     /locus_tag="PKV64_00420"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HPN15573.1"
                     /translation="MIGPLLTAAGIVFLYMTGLCVLALVRKNYSIVDAGWGPGFVLAA
                     AALFLRDPDFSPARLLMTALITLWAARLSFHILRRNAGKPEDFRYAAMRKRWGKAAPL
                     KSYIFIFLFQGAVMLLVSSPLTAVFLSPARPLSILDAAGTAVFLAGFLFETVADRQLA
                     AHIRDSGNKKMLMTRGLWSLSRHPNHFGEATLWWGLGLIALSSRYGWAGLIGPAVITF
                     FLRFVSGVPLLEKKYAGRPDWEEYKKKTPVFFPRLPGA"
     gene            complement(71890..73707)
                     /locus_tag="PKV64_00425"
     CDS             complement(71890..73707)
                     /locus_tag="PKV64_00425"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPN15574.1"
                     /translation="MSTTSQRSNGKSYYYAFLAGTKKILENQNELNRINVFPVPDADT
                     GSNLASTLRAVVERVRPHRSYGATSAAIAAAALEGARGNSGVIFAQFLYGLGAESRDW
                     TEVSPARFARSVLKAVRYMYEAIAEPVEGTMITVIRRWAEYLESREKEAENFLKVLAD
                     SRGRALEALQETRHRLAAMKKAGVVDAGSKGVVLFLEGAIDFLIKPSHKKIVSIRAEA
                     IEIEHTEAVSAAPVGRRYCVEALIRPHPGREAAGLDRDALSSLVSKAGDSLVIAGSRD
                     TLRLHVHADEPPEIFYAIRDFGLCVHQKADDMQRQYETVHRRKRNVALVTDSSCDLPG
                     LLLDHHQVHVIPLRLNFGPCSYLDKTTISPDRFYTMLDEDRGFPTSAQPNAKAFEDMY
                     ARLAAHYDSILSIHLSGKLSGTYAGAASVAERVSRKTGKKITVIDSRTLSGALGLVVL
                     RTARALEAGASHEEAAAEAERVRPRSRILVGVRTLKYMVRGGRVSPMAGRLANLLNLK
                     PVVSVKENGESIVSDKAFSRRGNLKNIRRQARKAAAGSRVWESCVLHAHNPEEAGIYA
                     LEMEEIFGRKPAYLMDISPVIGLNAGVGAVAVSFLMEEA"
     gene            complement(73723..74148)
                     /locus_tag="PKV64_00430"
     CDS             complement(73723..74148)
                     /locus_tag="PKV64_00430"
                     /inference="COORDINATES: protein motif:HMM:NF021462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HPN15575.1"
                     /translation="MILKYAVLWIVSFIGVSAVDAFWHLVLWGRIYRRDIRKVAAVVD
                     GKLVLNGISGALSQVLVITAFVVLASLQLSSADPYLKGILACAAGGVLGISAYGLVNH
                     WIVRDWSLSLTVLEVIWGPVLGAFAGFLIVGTARLLKIG"
     gene            complement(74435..75868)
                     /locus_tag="PKV64_00435"
     CDS             complement(74435..75868)
                     /locus_tag="PKV64_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPN15576.1"
                     /translation="MKSLKFLPIILILAGCLFLVDCARNETAVRTVPPPPGKAAAVPE
                     APPSEKAAPAPVQEATGKTIIPVEEKNLASGVLPVNGQAADDASITIEDVLELYAEAK
                     AARERGDFEGAVAYLDEAYAALLRLRIPADSPLIQDKDNLRLLIAQRIQEISAARRNP
                     AVENHKSIPLIENEWVLREIRSFTGPERTAFLEGYRRSGFYKDWIVDELQKAGLPSDL
                     VWLPIVESMYKPLALSSARALGIWQFIRTTGLNYGLNQDKFIDERRDPYKSTFAAIKY
                     LEHLHSFFGDWTTALAAYNCGEGYVQRIINSQNISYLDNFWDLFQRLPYQTARYVPRF
                     IAVVLIIRDPEKYGVSLPAPYPPLRFDTVRIQHPAKLSVLAAAMGLDSSELEFLNPEL
                     RYQSTPDTPYDLRVPVGMEERLLAALPALPKYIPPEYGTHKVRSGETLSTIARRYGTS
                     VQNLQKINGIRGTLIRAGQVLRIPSRG"
     gene            complement(75944..76957)
                     /gene="galE"
                     /locus_tag="PKV64_00440"
     CDS             complement(75944..76957)
                     /gene="galE"
                     /locus_tag="PKV64_00440"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPN15577.1"
                     /translation="MKKVLVAGGAGYIGSHTVKELRRAGFKPVVFDNLSSGHRDAAGG
                     AELIEGDLRDPDLIGRLLKEGGFSAVLHFASLIQVGESFVDPRKYYEHNLRTSLNLLG
                     AALDAGVRVFIFSSSAAVYGLPRETPIREDHPLDPVNPYGRTKAMIEQVLRDYDRAYG
                     LKHVSLRYFNAAGADPDGELGERHHPETHLIPNVLRAAAGDGSILGVFGTDFPTADGT
                     AVRDYIHVTDLASAHVLALRRLLDGGSSEVINLGTNRGYSVREVIAAAEKATGRRVPR
                     EDRPRREGDPPILLASKEKAERVLGWRLRFSDLGTILETAWAWHLKESGERKRPGGGE
                     AGR"
     gene            complement(76954..78414)
                     /locus_tag="PKV64_00445"
     CDS             complement(76954..78414)
                     /locus_tag="PKV64_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPN15578.1"
                     /translation="MPKLEPDFQDRRFFVRLFGDDVYAVGGFVRDILRRKPSGEIDLL
                     VTRRTLDEIIGILEPKGKVDLVGKSFGVIKFTRAGRTVDIALPRIDRAAVKSVRGHKD
                     IRAQSDPMLPVEEDLRRRDFRCNSLAWRLSDGALIDPFNGRRDIREKILRLTDPAAFP
                     EDPLRVLRAARFASVLGFAIDPEVYPIARKVDLSGLSVERIADELFKILLRSPRPSRG
                     LEELFRLGALRALLPELYALTLVIQDSVFHPEKDDFGHHTVWAHTKLTVDQAKRLAEK
                     LDLDPAKRPALLWAALFHDAGKAETTHWEYKRGRMAVTSAGHDTAGEKLALRVFDRLK
                     IHAWNGADVRDLALRLIRTHHRAAELWNNRTIVTKKAFNRFAADTGGEFELAAVLDAA
                     DRAGRAERPAAGLDREARWLLESMRKHNVSRETIRPLLQGRDLLKLNVPPGPGMGKLL
                     KDLYRRQLDNEFDTRAGALKAARRLVREQRPAKERP"
     gene            78503..>78993
                     /locus_tag="PKV64_00450"
     CDS             78503..>78993
                     /locus_tag="PKV64_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15579.1"
                     /translation="MKKAQVAAWLGLLAALSAFGQRAVPGVRLHEKRIVDPSPRGLTL
                     VFRLILRNPAETAVRLTRYDYQVVIGDAEYLKLQVPLPEPIRVEAGSEITIALPVKLN
                     FAHLFPVVPDLKDKDQAVCYVAGGLIFADDRGRERRVPVAFSGDFPVYRGLDFAPVPV
                     EAKS"
BASE COUNT        15121 a        24468 c        25585 g        13719 t
ORIGIN      
        1 gatcgtcttc tgcggcgtcc ggttcatggc cgaaaccgcg gccctcctcg ccccggataa
       61 aaccgtcctc ctgccggacg agggcgcggg ctgcccgatg gccgacatgc tcgaggccga
      121 agacctggtc gaatggaaaa aacaatatcc cggccggccc gtcgtcctct atgtcaacac
      181 gtcggccgcg gccaaggccg aagccgacct ttgctgcacg tcggccaacg ccgacgccgt
      241 cgtggcctcg cttccggaag aggagatcct cttcggaccg gacaagaacc tggccgcttg
      301 gacccagcgc cgcacggcca agcggatcat cgcctgggac ggctggtgcc atgtccacca
      361 gggcatcctt ccccgggacg ttgcggcggc ccggacccgg catccgggag ccgtcctctg
      421 ggctcatccc gaatgccgtc cagaggtcct ggacctggcc gaccgcgtcc tctcgaccgg
      481 cggcatggtc cgggaggcgg cggcctccgc ggccgccgac gtgatcatcg cgaccgaatg
      541 cgggatgctt caccgcctcc gccgggacca tccggacaaa aggttctttc cggtgaagga
      601 aaacgcctta tgtccgaaca tgaaaaaggt cacgctgccg aaaatcgccg ccgccctgga
      661 gacgatggcc ccccgggtga ccgttccgcc ggagatcgcc gcccgagccc ggcgggccat
      721 cgagcggatg atccggcttt gaggaccccg agcccgaaat gacgaaagcg gagagttccc
      781 gcgaaaaagg aaaggacgga acgcctcttc cggcggcaga ccgccttccg gaaatcatcc
      841 ggatcctgaa gagggaatac ccggatccga agcccttcct ccatttccgg acgccgctgg
      901 aaatgctggt ggcgacgatc ctttcggctc agtgcacgga caagcgggtc aacctggtca
      961 ccccgggatt gttcaagaaa tatcccgacg cccgggctta cgccgaagcc ggtccgggcg
     1021 ttctggaaaa cgacatccgc tcgaccggct ttttccgcaa taaggctcgg aacatccgcg
     1081 gagcggcccg taaaatcctc gacgacttcg ggggcgaggt cccggcgacc atggacgagc
     1141 tcttgacgct tcccggcgtc gcccggaaaa cggccaacat cgtcttgagc aacgcctttc
     1201 ataaaaacga agggatcgcc gtcgacgtcc acgtcggccg ggtctcccgg cggctggggc
     1261 tgagcggaca cgaggatccg gtgaaaatcg agcgggatct cctccgcctc gttccccgga
     1321 agcattgggg cgatttcaat ttctgggtgg tcagccacgg ccgggcggtc tgccgggccc
     1381 gcaagccgga ctgcggggcc tgccccctcc ggcccggctg cccttttcca tcgtcgccgc
     1441 ggccgcgatg aacgcctccg ggcggccggc ggctttgaag cgggcttcgg tttcgactac
     1501 aataaaaaca tgaccatccg tccaaccggc ggagagaggc cgggatcatg gtgaggcggt
     1561 tttcattcga cgacgaaacc ggggattcaa ccggcgtcca taaggcgcgc ctctggtcga
     1621 cggccgggga caacgccgtc cgctgcgcct tgtgcgccca ccgctgcgag attcatcccg
     1681 gccatttcgg cgtttgcgga gtccgggaaa accgggccgg ccggctcgtc acccgggcgt
     1741 acggcgaggt ggtgagcgcc ggggccgatc ccatcgagaa aaagccgttt ttccattttt
     1801 tccccggatc gacggctttt tcgatcgccg cggccggatg caacttccgc tgccgcttct
     1861 gccaaaactg gcagatttcg cagctgcgcc cgatcgacgg ccgcgcgtcc ggaggccgcc
     1921 gcttggcccc cggcgacatc gtccgcctgg ccgcggccaa cggctgccgg agcctttcct
     1981 tcacctatac ggagccgacg attttttacg aatacgccct cgacacggcc gtcctcggcc
     2041 gggcggccgg cctgatgagc acgttcgtga ccaacggtta cctgacggcg gaggcggtca
     2101 aggtgatccg tccttttctc gacgcggcca acgtcgatct caaattcttc cgcgacgaaa
     2161 gctaccggaa aatctgcggc gcccggctcc agcccgtctg cgacacgatc gccctgatgc
     2221 ggcggctggg gatttgggtc gaggtgacga ccctggtcgt ccccggcctc aatgacggcg
     2281 aggcggagct gcgcgacatc gcccggttcc tggccgatgt cgaccgcgac atcccctggc
     2341 acatcagccg cttccacccg gattacgagt accgggaagc cgtcccgacg ccgatcgaga
     2401 ctctccgccg ggcggccgac atcggccggg aaaagggtct ccgtttcatc tacacgggga
     2461 acatctcggg ggaaatcgag ccgacgcgct gtccccgctg ccggacgacg ctcatccgcc
     2521 ggcgcgggtt cgaggtgatg gaaaaccggc tcttaaacgg ccggtgtccg cagtgcggcg
     2581 aagctttgcc cggcgtcttc ccggcggccg ccgttccgtc gcccctctga tatcaagaag
     2641 gagattctct tggccgcatc ccatcaaagc tcgggcccga tcgccttcga ggttctccgt
     2701 caaggccggg ccgagccgtg aaaatcgtcc tggccggtta taacgtcgac cgggaagctt
     2761 tggacgaagt gctccgagcc ttccccggcc gcctcgacct gacgcctgaa acgctgtcgg
     2821 cggcctatgc ccggatttcc cgcgaccccc gcccggctga tgagctccgc cgggccgccc
     2881 ggcgggaagt ggaaaaggcc cggcggtcca acgcggcgat cattttcaag atgggccacc
     2941 attccgtggc cgagcatgcc gttttcaatt tcgacctcat ggggatcagc cgcctggcca
     3001 tcgaggaaat cgaaaaattc cgcctctgtt cctttaccga gaaatcccag cgctacatca
     3061 agcttggaga cgattacgtc gtccccgagg aaatcgtccg ggcgggcctc ggggacctgt
     3121 tcgtcgagac ggtccgggct caaaacgcgg cttatcacga cctctatcgg atactccggc
     3181 ctcttttttt tgaaaaccat cccgccctgg cggccgatcc gaaacatcac ttcctgctgg
     3241 acggctgggc caaggaagac gcccgctacg ccgtcggcct ggcgaccgag gggcagatcg
     3301 gcctgacgat aaacgcccgc aacctcgagc tgatgatccg gcggctggcc gccaaaccgt
     3361 cggccgagat ccgggcgatc ggtcttcact tggccgggga agcggcccgg gtcgcgccct
     3421 cgatcgtccg ctatacggaa gccgcggcgt atgacgccga aacggaagag gccttccggg
     3481 acgaggccgt cgagagcctc gggagccgtc cgtccgccgc ggccgatcga agccgccgga
     3541 gcggttccgt ctcttccgcc gtccgcatgg ctggggcgac gcccgaaggc gacgagcgcg
     3601 tcctggccgc tcttttacac gcttcttggg gaggatccta cgcccgctgc cttcgggaag
     3661 cccgccgtct tccggttcag aagaaaaaag cgctcttcct ccgtgttttc gagcgcatgg
     3721 aattttacga ccttccgccg cgcgcctttg agcacgccgg gctgacgttc gagctgaccg
     3781 tgtcggcgtc ctgttacgcc cagctcaaaa gacaccggat ggccaccttg acggcccagg
     3841 cttacgatcc cggcctcggg gtgaccgttc ccccctcgat cgaggcggcc ggggaagcgc
     3901 cgcggttccg ggacgtcatc gggcggaccg aagcggctta ccggatccta aaggaggccg
     3961 ccggcccggc cgcggcttac gttctgacca acgcccaccg ccgccgggtt cttctcgccc
     4021 tcaatctccg ggagctcacc catctggccc gcctccgcga ggatgcctcc gcccagtggg
     4081 atatccgccg tctcgcggcc gagatgtcgg ccgccgcccg ggcccggttc cccctggccg
     4141 cgatgcttct ctgcggcaag gacgcttacc cggatgtcta cgcgaaaatc ttcggccgcc
     4201 cgccggacaa gacgcccccg tcctccttga aaaacgggta gcgttttatc cggggccggc
     4261 cgttccagcg gacggggaat ccgcggctaa tcgtcggcga cgacgcggtg gatttttttc
     4321 tttccggcct tgaggatcat cccgtcgggc ccgatgtccg ccagggtcag cttccggtcg
     4381 agggaatcga tcttctcgtt gttgaccgag aggccgccct gggcgatcaa ccgcttggct
     4441 tcgccgcgcg agggggccag gccgatttcg gcgaagagct cggccggagc gatgccggct
     4501 tcgaggcgtc cccgggccac ggccgtcgtc gggagcgagg cggcgtcctt ttgaccggag
     4561 ccgccgaagg cggcggccgc ggccgcccgg gcgtcccggg cggcgtcttc cccgtgggtg
     4621 agggcggtcg cctcgtaggc caggatgcgc ttagcctcgt tgagctcgct gccggcgagc
     4681 gattcgaggc gctggatctc gccgagcggc aggagcgtgt agaggcgcag gaagcggccg
     4741 acgtcccggt cgtcggtatt gatccaatac tggaaatagt cgtaaggcgg gcagagactc
     4801 ccgtccagcc agacggcgcc ctgggcggtc ttgcccattt tcgccccggc cgaggtcgtg
     4861 aggaggggaa acgtcagcgc ctcgaccgcg gctccctcga cccggcggac gagatcgacg
     4921 ccggccagga tgttgcccca ctggtcgtcg ccgcccatct ggagggtgca gccgtaggtc
     4981 cggaacaagg tcaggtagtc ataggcctgg aggagctggt aattgaactc gatgaaggat
     5041 aagccccgtt ccagacggat cttgtaggct tcggcggcca gcatgcggtt gacgctgaaa
     5101 tggcggccga tgtcccggag gaattcgatg tacttcaacg gcaggagcca gtcggcgtta
     5161 ttgaccgcca gcgccccggc gccgtcgaat ttcaggtacc ggtcgagctg agcccggatg
     5221 gattctccaa aagaccggat ggtcgtctcg tccaggatct ggcgcatctc cgtccggccg
     5281 ctcgggtcgc cgaccatggt ggtgccgcct ccgatgaggg cgatggcccg gtggccggcc
     5341 cgccggaggt ggatcatggc catgatcgga accaggcttc cggcgtgaag gctggcggcc
     5401 gtgccgtcga agccgatata gaacgtcgtc tttttctcgt cgagcatttt cctcacggcc
     5461 gcttcgtcgg acacctgggc gatgaagccg cgagccttca attcattaaa aatcgattcc
     5521 gtcacgggag tcctctcttt cgaacctctc ttttatagca caggaccggc cggacggaca
     5581 aacgcccgtt cccccgaaat gctccggccg gcggccggcc ttgcccggcc gtgttgccga
     5641 tgatagagtg ggggcgggag tcggaccgct catggaaaaa cgctaccgcc ggatgctccg
     5701 ggggatgatg gtcaaggaag agggaacccc cgacgccccg gggcccgcct ggtttcttta
     5761 tatcttgaaa tgcagcgacg gctcgctcta caccggcgtc accaccgata tcgaccgccg
     5821 gctcatgatg caccgggagg ggaaggcctc gcgctacacc cggacccgcc ggccggtgga
     5881 gctcgtctac cgcgaggagt gcggcagccg gtcccaggcc ttggcccggg agtgcgcggt
     5941 caagtccctg ccccgggcga aaaaagaaag gctcttcgac gctccgaggt gacgcccgcg
     6001 gcgcgaaagg cccgccggga aaagagagcc ggcccgaggc gctccctcgg ccggccgccg
     6061 gaaaccggtt tcccgctcaa ggacctgatc gcccggggcg tcccggtgaa ggttcgccga
     6121 ttccgcccgg caagaggcgg ctttcacaag aatcagccgt acttcaaggg agtggagacg
     6181 tcgagcctgc cggaatcgac ggcttgggtc gccggcggcg acgaggtgat gatgttttag
     6241 gcctgcgttt ccaccaggga gaaagggatg gaaagcgatt cgtccgatta caggatcgtg
     6301 acccggcgcc cgcggatttc gagccggcct tcgaaataca ggcgcgccgc ttcgggatag
     6361 atcttgtgct cctcgaccag gatgcgggcg gccaaggttt cagccgtgtc gttttggaga
     6421 acggggacga cggcctgcag aacgatcgct cccatgtcga cctccgccga gacgaagtgg
     6481 accgtgcagc cggaataacg gacgccccag tcgatcgcct gctgctggac gttgaggccg
     6541 ggaaacgacg gcagaagagc cggatggatg ttcaggatgc ggttcttgca ggcggcgcag
     6601 aactccggcg tcaggacctt catgtagccg gccaggcaga tcaggtcgat gccccgggct
     6661 tcaacctcgc ggaggatcgc ccggtcgtaa gccgccttgt cggcgaacgc cttggggctc
     6721 aaggacatcg tcgggagccc ccgcccgcgg gccgcggcca ggccgggcgc gtcctccttg
     6781 ttgctgaaga cgagggcgat ctcggcgttg atctttccgg ccgcggccgc gtcggcgatg
     6841 gccgtgaaat tcgagccgcg gcccgaaaga agcaccatga tccggccgtg tttcttggga
     6901 ctgcggaaga cttcctcgct catgggaccc tccttccccc gggggggtcg atctccaaat
     6961 ggtctttcgg aacccagcct gttttccccc ggtcgtccgt tccccaaacc catccggcca
     7021 gctcgccgag gggacggatg gtatctccgg cttcgaccga aagctccgtc gcgtcgtaat
     7081 cccgggttaa gaaagcctct tcgccttccc ggcggagata ggcgtccggg acccagcccg
     7141 ttttcccgtc atcgccgcgg ccccataccc agcccggcca ttcgctcgtc cggggcagaa
     7201 tcgtcacccg ggctccgcgg gcgaggcgga tcgggtcgga atacggcgtt tcgtatcgtt
     7261 tcgcgactcg atacagcgtg cccacggccg gtctcaggag ggcctctttg cgggcgcgaa
     7321 gaaatcgggg atggcgttca tccaaagccg gcgggcttcg gcgaccggaa gatcgacaag
     7381 aaggcggccg gagacccgga agcggagggc gcgtccgccc gttgtcccga cgatccgggc
     7441 ggggacgccc cgtttttcgg cgatctccag gacgcgccgg gcgttttccg ggcgggaggt
     7501 caacaggatc cgggcttggc tttctccgaa gagaacggca tcgggccgga gatccgtctc
     7561 aaaggacagg tcgcagccgg ttccttccgg gctgtggaag gcgcattcgg ccgcgcagac
     7621 ggccaatccg ccttccgagg gatcggcggc ggaacggacg agcccggcgc cgatcgcttc
     7681 caggagcgtc tcgtggacgg cgatttcccg ctcgagatcg acggccggcg gcgccgcgcc
     7741 tcgttttcca tggatgagct tgagatattc cgatccgccg aactcctcct tgttttcccc
     7801 cagcaggatg accgcgtccc cctcggcctt gaatcccggg gtgacgagcc ggtcgatatc
     7861 ctcgatgagg ccgacgattc ccaggacggg cgtcggatcg acggccgcgg cgccggtctc
     7921 gttatagaag ctgacgttgc cgccggtcac ggcgaggccg aaggcccggc aggcttcgct
     7981 cagcccctcg acggtctcct tgaactgccc catgactccc ggtttctcgg gattgccgaa
     8041 gttcaggcag ttggtcaccc cgatcggccg ggccccgacg caggccaggt tccggcaggc
     8101 ctcggcgacg gcggcttttc cgccggtccg gggatcgagg gccgcatacc ggccgttgcc
     8161 gtccagggtc atggccaagc ctttcttcga gccgcggatg cggaggacgg ccgcccccgc
     8221 gcccgggaga agaacggtgt tcgtcagaac catgtggtca tactggccgt agatccattc
     8281 cttgccggcg atggtggggg aggaaaggag ccggcggagg acgtcggcgt aatcggcggg
     8341 cggggggacg ggcccggccg cccgggcctc cggggcggcc ggccgtttcc aaggccgctt
     8401 gtaggccgga caaaggtcga cgacggccga gacgggaacg tcgacgacga catccccgtg
     8461 gaagcggcac gtgagccggc cgtcgccggt gaccgcgccg atctcgacgg cgtcgagatc
     8521 ccacttggcg aaggccgcct tgaccgccgg aacctggtcg ggccgggcgg cgatcagcat
     8581 ccgttcctgg gattcggaca agaggatctc ataaggcgtc atgccttctt cccgctgggg
     8641 gacccgatcg aggtcgatgg ccgcgccctt gcccgattta gccggcattt cggtggccgc
     8701 gcaggtcagc ccggcggcgc ccatgtcctg gatcccgacg atgattcgcc gctccatgac
     8761 ctcgaggcag gcttccatca agagcttttc cttgaacgga tcgccgacct ggacgttggg
     8821 ccgcttggct tcagtgtccc cgccgaattc ctgggaggcc atcgaggctc cgtggatgcc
     8881 gtcgcggccg gtcttggccc cgacgtagag aaggacgttg ccggcgtctt cggtccgggc
     8941 gtagaaaatc ttgtcctttt cggccaagcc caggcagaag acgttgacca ggggattcag
     9001 agaatagccc tcgtcgaaac aggcctcgcc gccgacggtc ggcacgccga tggaattgcc
     9061 gtaggaggag atgccggaga cgaccccttc catgatcgag cggttcttgg gattatcgag
     9121 cggcccgaag cggagggaat ccatcagggc gaccggccgg gcgcccatgg tgaagatatc
     9181 ccggagaatg ccgccgacgc ccgtggccgc gccctggaaa ggctcgatga aggagggatg
     9241 gttgtgcgat tcgatcttga agacgatgac ccggccttcg ccgatgtcga tgaccccggc
     9301 gttctccccg gggccctgga cgacgttggg ggcccgcgtc ggcagcgtct tgagaaagac
     9361 cttggagctc ttgtacgagc agtgctccga ccacatggcc gagaagaggc cgagctcggt
     9421 caggttgggt tcccggccga ccagcttgac gatgaggccg tactcgtcgg cggtcaggtt
     9481 gtgccgggcc aaaaccgtgt cgtcgtggag gcgcgttttc atggcctgtt ctcgagaagg
     9541 gacgtcagga cgacgagacc gtcgacgctg ccgagaaccg gctcaacggc ccgttcgggg
     9601 tggggcatca gcccgcagac gtttccctcc ttgttgatga gtccggcgac ggcgtgaagc
     9661 gagccgttga cgtcggcctc ctcggtcaga gcgcccgagg gggcgcagta ccggaagacg
     9721 acttggccgt tgtcctcgac ctccttgagc gtctcgggcg gagcgtacca gttgccgtcg
     9781 aaatgggcga tcgggatgcg gagaatctgc cccgggcggg cgttccgggt gaacggcgtc
     9841 cgttcgtttt cgacccggat cgtcacctgc cggcagagga atttcagctt cttgttccga
     9901 agcatggccc cggggaggag attgagctcg aggaggactt ggaagccgtt gcagatgccc
     9961 aggaccaggc cgcccttggc ggcgaaggcg cggacttccc gcatgagcgg cgagaaccgg
    10021 gcgatcgccc cgctccggag gtaatcgccg taggagaacc cgcccgggag gatgacgcag
    10081 tcgacgcctt ggaggtcgtg atccttgtgc cagaggtatg ccgtcggttg tcccaggacg
    10141 tccaggcagg cgtgaaaggc gtcggagtcg cagttggatc cggggaagac gaccacgccg
    10201 aatttcacgg atgcctcctc agtcttcgag gatctcccag gaggacttct caatgaccac
    10261 gttggccagg actttgccgg cgatctccgg cagcaggcgc tcggcttcgg tccgggagac
    10321 gccccggagc tcgatctcga agaccttgcc ctggcggacg tcccggacgg cctcgtagcc
    10381 gagggtgtgc agggcgttgc ggacggcctg gccctgcggg tcgaggaccg aatccttgta
    10441 ggaaaccagg attctagctt tcacgggaga gcacccgctt gaagatgtaa tcgatcttct
    10501 cgaggtaggg gtcgaggctg aagcacgttt cgatttcggc cggggagagc cgctcccgga
    10561 tccgggggtc ggccaggacg agggctttga aatcgaggtt ttcctcccag gttttaaggc
    10621 tgtttctctg aacgagggcg taggcctcct cgcgggtcaa gcctttccgg gtcagggcca
    10681 gcaggacgct ctgggagaag gtcaggccgc gggtcgcgtt gatattggcc ttcatccggt
    10741 cctttcggac gacccagtgg gagatgatat cgatcgcctc ggcgagcatg aagtccgtca
    10801 ggatgaacga gtccgggaag atgatccgct cggccgagga atgggaaatg tcccgctcgt
    10861 gccagagagg gatgttctcc agggccgtct ggacgttcgc ccggacgagg cgggccaggc
    10921 cggccatccg ctcgcaccgg acgggattct tcttgtgggg catcgaggag gagcccttct
    10981 ggcccttggt gaacggctcc tcgagctcca gaacctcgct tctctggagg tggcggatct
    11041 cgacggtgaa tttttccagc gacgcggcga tcagggcgag cgtgccgatc gcctcggcat
    11101 ggcggtcgcg ctggaggacc tgggtggaga ccggagcggg cttgaggccg agcttcttga
    11161 gggccaggac ctcgacgcgg ggatcgagat ggatgtaggt ccccaccgcg ccggagatcc
    11221 ggccgacgct catgacctcg accgcccggt cgatccggtc gagatgacgc ttgatctcct
    11281 cgtaccagac caggaccttg aagccgaaag tcaccggctc ggcgtggacg ccgtgggtcc
    11341 ggcccatgca gacggtcttc ttatgcttga gggcttcctt ccggaggacc ttcttgaggg
    11401 tgaggaggcg ggcgcggacc tggaggagcg accggcggac caggagcgag agggccgtgt
    11461 cgacgacgtc gtaggaggtc aggccgagat ggatatagcg gccgtccggc ccgacttttt
    11521 cattgaccgc cgtcaggaaa gcgatgatgt cgtgcttgac gacacgctcg atctcatcga
    11581 tccgcttgat gtcgaaggcc gccttggcct tgatcgtccg gagggacgag gccgggatct
    11641 tccccagctt gttccaggcc tcgcagacgg cgatctcgac cgcaagccag gtccggtagc
    11701 ggttctcctc ggtccaaacc gcccccattt cgggattcgt gtagcgttcg atcatgggtc
    11761 acgttccttg cggggggatg ggcgggagca tcataccaac agggggcggg ggagtcaatt
    11821 tcagccgcgg tcgaccagcg tgattccgaa atcgtccagg gtgaccaggc gttttttgta
    11881 aaggcgcccg acggccgttt tgaaagcgtt cttgctcact ccgaaaaggc ggccgacgaa
    11941 ttcggccggc gatttgtcgg tgacctccag gaagccgtcg ttggcccgaa gcgcgtccag
    12001 gatccggccg gccagctcgt cgacgccctc ggggcccggc ttgcccaagc ggaggtcgat
    12061 cttcccgtcg tcccggatct tccggacgta tcccttgacc cggtcgcccg gccggagctc
    12121 ccggaagacc tcgtttttat agagaatgcc gccgtgggaa ttatcgatga ccgccttgta
    12181 gcccagctcc gtccggccgt aaatcaacag gtcgacttcc cggccggcct cgaagggcgg
    12241 gggcgtcttg gccagatgct tgtcgatctt ggccgaggcg gcgatccgcc ggctggcctc
    12301 gtccaggtag acatggacga cgtatttttt cccggccttc atcggcggcc gttgctcgct
    12361 ccaggggacg agaaggtcct tggggaggcc ccagttcagg aacgcgcctg tgggattgac
    12421 cgcgaccgcc tcgagaaagg cgaaatcccc gacctgggcc aggggctttt ccgtcgtggc
    12481 gatcaagcgg tcctcggaat cgaaatagag gaagacgtcc aggcggtcgc cgggggccgc
    12541 tccggccggg acgtagcggc gggggaggag gatttcgccg aggcctcccc cgtcgagata
    12601 gacgccgaag gcgaccgtct tgatgatcgc cagcgagttt cgttttccga tttcggccat
    12661 tccggagtca ctatacccgc cgggcgcggg ggatgcaaac gcggcggcgt cggaacgccg
    12721 cgggggcctc ggttattgag tcgcttcgcc gctttcggag tctgggaaat gggcgtgagc
    12781 cgggctcttc gccgcctcgt agatccgttc gacgtaatgg acgaaggcga cataggcttg
    12841 aacgtaggcc cgccccttct cgacatcgtt ctcttcgtac gctttccttt cgaggacttc
    12901 cttgaattgt cccccggctc cctcccggac gagatccgac aggagattca gaagatcctg
    12961 ggccgaaccg ctctcaacgg cccggtcggc ggccgggatg gccggcccga ggtctcgacc
    13021 ggcgggtttc aacccggtgt aaggcgctcc ctccccggcc cggtgaagac ggacgagcgt
    13081 ctcgaagaaa tacatgtcgg ccaactccgc ggcctgttcg ctcaatttcc ggacggacag
    13141 cgttttctca aaggccttta tcacggcctc ctcgtcctgc ttatgtaccc agatcaaaac
    13201 gagacggacc tctccggttt ccagggcttt ttgggcggcc ttgacgaccg gaccgtccat
    13261 cccgtcgcaa tgggcccgga gaagcggagc cgcgatgatc gggagaaaga ggccgagagt
    13321 caggagaaat cggatgacgc ttttcctttt cattttaatc ttccttttta agacaggtat
    13381 tcttccttca agcggtgaag gaaggcgtgg aattcctcgt ggcggcccgc tccgacgacc
    13441 tcgtcctcga tcttctcaaa gcccgcttcc agcttcttcc agaccccggc cggcaaccgg
    13501 ctctcggcca ggggatagag gacgttgtcc tccttttcga tatggcgggc caggagggag
    13561 gcgtacccgc gcgcgttttg ggcgtattcc cggccggacg ttcttccgcc cttccgatac
    13621 tcgagaaagg cggcgcccat ccctttcaca tacatccgtc cttccttgtg ctcggccaac
    13681 attacgccga gcggcccgcc ttcgcgggga acccccgccc gctccatggc cggaaacaga
    13741 agatcctctt cctttccgtg gtggcaccgg tcgacgaaga ccctcagaaa ttccaggagc
    13801 ccgccgaggt gatcttcctc gacgctgacc ccttcctcca ggcgccgggc cgtctcctcc
    13861 aggattttca gggccagctt gacggccgcg tgctcgtttt tcagatcttc ggtcggcttc
    13921 atgtcgccct ccttatcgaa atggaaatct ccgcttcccg cgccgccgcg gacgaagcgc
    13981 cggcatttga ggcggcggat tttcggtccg gtcgaatccc tccggcgccg tcaacgccgc
    14041 cgcggacgcc gtcgaaaacg ctttatctcg gatgaatttt tttcaacacc gtttctgcat
    14101 gattcaatat aggttcgggg cggcggtccg tcaagccggg acggaggaca tcctgctttt
    14161 gacaggcgcg gccagcctcg tgtaccctct ggccatcgca tccgcccatg accggccaga
    14221 aaggaattcc atgaccccct catcccgtcg ccgtccgtcc gcccttcttt tgatcgcctt
    14281 gatcgccgga tgcctggttc tggccgtgga ctgcgcgccc ggcccggcct cggttcccgc
    14341 cgaccttgtc ctgaaaaacg gcgtctttta caccgtcgac gcctcttcga gccgggccga
    14401 ggccgcggcc gtcaaggacg ggagattcgt ctatgtcggg agcgactccg gcgcctccgc
    14461 cttcatcggt aaggcgacaa aggtcgtcga cctggagggc aagctcgtcc tgccggggtt
    14521 catcgacagc cacgcccaca tcatcagctc cttccggcgt ttcttcgagc tgaacatctt
    14581 cggccggacg acggcggagg aggtccagaa agcgatccga gactacgcca cggcccgtcc
    14641 cggggccgaa cggatcagcg gccggggatg gagcaacacg gcgttcccga aaacggggcc
    14701 cgaccggagg atcatcgatg ccgtcgtccc ggacatcccc gtctgcctag cctccgagga
    14761 cggacacagc cgttgggtca actccaaggc catggccctg gccgggatca caaaggagac
    14821 gaaaaatccg cccggcggct ttatcgagcg cgacccggcg acgggggagc cgacggggac
    14881 gctccgggaa agcgctcagg atcttgtcgc cggactgttc ccgtcttaca ccgtcgatca
    14941 attgaagcag gggatcgagg cctaccaggg gatggccgcg gccttcgggc tcacgaccgt
    15001 ccatgaggct tcccttgatt cctggagcag cgagatcccc gcctccgaga tccacgcggc
    15061 cagggtggtt ctcaccctgt tcaggggaaa gacggtctac gcggccggcg aaggcgtcga
    15121 gcgccgatga acccgagaat caagcttctc cgcggcgaca tcacgaagat tgcggccggg
    15181 gcgatcgtca acgccgccaa cggttcgctt ttagggggcg ggggcgtgga cggggcgatc
    15241 caccgggcgg ccgggccggg cctcctggcc gagtgccgga cgctcggcgg ctgcgagacg
    15301 gggaaggcga agctcaccaa gggatacaac ttgccggcgc cctatgtcat ccacgccgtc
    15361 ggccccgtct ggaggggcgg aaccggcggc gaagaggcgc tcctggccgg ggcgtaccgg
    15421 agcgccttgg atttggccgc ggagaacgga atccggtccg tcgcctttcc caacatcagc
    15481 acgggcgttt atggataccc gaaggataaa gccgcggctg tcgcgatccg gacggtctcg
    15541 aatttcctgg acgggcatcc ggagatggac aaggtgatct tcgtctgctt cgacgaggac
    15601 aatcaccggt tgtacgaacg gaggctcgga ggatagcggg cggatccttt tttccgatag
    15661 ggaagcgcgt tgattgtctt cggacaaggg cggcgggagg cgctccggtc tcctgttcag
    15721 tcccgcccgt tttctcaggt tcgggcagac ggggagccgg aggcgccagg agaagggaaa
    15781 agccttgccg ccggaccccc ggagggccgg ggatccggcg gccggcggaa accggctctt
    15841 acttgatcgg tctcatctgg tatttttcgt aaaggccgtc ccagtagttc ttcatgtcga
    15901 tgtgacggcc gccgatgaac aggtgccgga cttcggagcg cgtgtcgagc ggatcgcccg
    15961 tggtgatgaa gaggtcggcg tccttgccgg tctccaggct ccccagccgg tcttcgactc
    16021 cgaagatccg ggccgggttg atggtcaaag ccttgatcgc ctcctcgtgg tcgagcccgt
    16081 aggccacggc ccgggcggcg tgatagggaa ggtccttgcc cgcggccggg tcgtctcccg
    16141 aggagaaaca gatcggaacc ccggccttga ccagctcgat cacgtttcgg aagggcgcgt
    16201 cgtagccgtc ttccggctcg acggggccga cgtacaggga atcgatgatg accgggatcc
    16261 cggcctcctt gattttcgag gcgactttga atccctggct gcagccccgg aagacggcct
    16321 tgagcttctc ctccttgatg aacttgaggg ccagctcgat gtccttttcc ttttccacgg
    16381 agaggatgac cggaagcgtt ccgtccagga caggggccag ggcctcgaac ttgcggttga
    16441 gagccggcgc ctcgagccgc gaatcggccg cggccgcctt cttgaggtcg agataacggc
    16501 gggcttcctt gaggtattcc gatattttct ccaccagttt ggacgtgacg tcgaccttgc
    16561 tctcctgtgc cgtcccgcca ccctcgcccc cggccggccg gggagacatg gggaagttga
    16621 ccagggaggt ggcggtttcc ttgagggcca tctcggccac ggtccagccg tccatgcgga
    16681 cgagagacga ctggccgggg aacgttccgc cggccggagc gaccagcgcc gccgtggtac
    16741 cgttgatccg gctcgttccg aagtggatgg acgcgggatt gatggcccgg gcggcgagaa
    16801 cctcgggatt ttccttgccc atttcccgga catcgaccgt gccggagacg gccgaaatct
    16861 cgaccaagcc gaaatgagtg taggcgtcga tgaacccggg ataaacgaac tggccggccg
    16921 cgtcgatgac cagggcctcg gccggcgcgt ccacgctcgt tccgagggcg gcgatcttgc
    16981 cgtcccggat gagaagctga cccgcggcga tcggcgcgcc ggtgaccggc acgagggtcg
    17041 cgttcttgat caggatcgtc cggccgtcct ccgctccggc cggtccggaa aagacgagaa
    17101 ccgccagggc ggtgatgacg aagacaacga gaagaaatat ctttttcatg gtcgtctcct
    17161 caggactttt tttccttggg ggcctcggcc gccgcttttt tcgcctcctc ggctttccgg
    17221 cggtcgtcga gatacttggc ccggtcgaag tagactttcc cgtcgacgat cgtcatctgg
    17281 cagacggtgt agggatcaag gggatggcgg ttgaaaacgg cgatgtccgc gtccttgccg
    17341 gcctcgagcg ttccggtgag cttgtccact cccatgatga tcgccggatt gagggtgatc
    17401 gttttgagag cctcgagctc gctcagcccc ccgaagcgga cggtcttggc cgcgtccagg
    17461 aagaggtggc gcatccgttc cgcatcgtcg gaattgaggg ctaccaggac gccccgcttg
    17521 gccatcaggg ccgccgaatg gggcatcgtg ttgaaggcct cccatttata agcccaaaaa
    17581 tcggcgaaag tcgaaacggc cacgttgttt tcgacgagct cgttggtgat tcggtagccc
    17641 tccaggccgt gctcgaaggc cgccagcttc accccgtact cccggcagag gcggatgatc
    17701 gaggccattt ccgggccctg gtaggcgtgg cagcggaccc acaacttgcc ccggaggatg
    17761 tcggccaccg cctccaggcg atagtccttc cgcggcggca gggggacggc cgcgcccttc
    17821 cggccccggg tctccttgat cttcttttca taggtttccc aggttttcat atacgcgcgg
    17881 gcttggtcga attccaggcg gatggattcc tccacgccca tgcgggaccg gggatagcgg
    17941 ggtttgcccg cgcccgaaaa gttcgtttgc ttggggtttt cgcccagggc ccacttgctc
    18001 gtcaggggcg cgcccttgaa caggaggccc tcggcgtccg ctccccaccg cagcttcagg
    18061 cagacgcctc ggccgccgat gggattggcc gagccgtggg tcgtatgaat cgaggtcacg
    18121 ccgcccgtta aggcgtagaa aatattcagg tcctcgggat tgagaacgtc ccggatgtcg
    18181 acttcggccg tgatgagatc gcccgtttca ttgactccgc cctcgatggc gatatgggaa
    18241 tgggagtcga tgatcccggg catgatccac cgccccttga ggtcgatgac ttccgttccg
    18301 gcctccgccg gaagatcctt gcccagcttg gcgatcttgg tcccgatgat caggatgtcg
    18361 ccgtcgaaat tctccccggc gatgggaaga acgtgggcgt ttttgagcag gagccgctct
    18421 tcggcggccg ggagcgcggc ggcggccgca agaagcccga tcagggcgag aataaagaat
    18481 ttctttttca tggctttctc caaggcctca ggcgggattc cgctttcccg tgaactcggt
    18541 cgagccggat gtcccttgaa cggccgtccc ccgcatcgtg tctccggtca ccgtggccga
    18601 gaaatagaga tcgatgtcct gtccgccgac gctggcgttc acctcgaaat agacctggtc
    18661 ggccgaaacc gatccgccgg tgaaatcgaa cgccccgaac ggcgtggtca tcttccccga
    18721 cagggaggct tcctcctggg cgaaatccac ggtcagggtc agcccggctt cggggaggga
    18781 cagctcccag cggccggaga cgttgaccgt cggcttttcg ccttccttgg ctttgggttt
    18841 cgtgaagtcg aacagccggc cgtcggcgaa aacgtatttg accttgggct ccttggccag
    18901 gggatccccc tcgaccagaa cgaggttggc gatccggccg gcctcgaccg cgcccagggc
    18961 cttatccaaa cccaggaagg cggccggaga ggacgtcagg gcttccaggg ccttgtccgg
    19021 ggacagcccg gcctcgaccg ctttttggac gccctccatg aagctcttgg gatcgtccgt
    19081 gccgagggag gagaaagcga acgggatgcc ggctgaggcc agggcggccg gattccgcgg
    19141 atagaactcc ttttcggctt tttccttttc agccttgccc agctggctga agcggctcgt
    19201 cgccggcgcc ttgaaggcga ccgtgcagag aatccgggcc ttggccttct ttaattcggg
    19261 gacgactctt tgggcttcgc cgcccaggtc gcagagaaaa tagtccagct tcagctcctc
    19321 ggccagggcc agcgaccgcc ggatgtcatg ctggttgttg cagaagaaaa cgacgggttt
    19381 cttgccagag gcgtactccc ggagaatgtc ccaccggccg ttatagacgg gccggcggat
    19441 gcccgcgggc atcttcgccc agagatcggc gttcaggccg taatgcccga tgtcggcgaa
    19501 ttcctggcga aagaaggcca ccactcccat gagcgagttc ggatagacca tgaaggaggc
    19561 gggggcataa cccaggccga gccaggtgtc cttgagcagg agggctttat tcttgtcctc
    19621 cgagctgagg gagaagagcg aggcccgccc ggacaaaacg cccttggacg gaatggcttg
    19681 gacggcggtc aagccgagtt tatgatattt ttcgagggtc gacttgccca ggacggcgaa
    19741 gtcgaacgcc cgaagctccg gcgtcaggcc gcggtccttg tccgtgaact cgcccgaata
    19801 gaacttcgcc gggtcgaatt tttcctcggg gagcttgagc agcgactggc ccaggccgtc
    19861 gatgaggccc gggtgcaggg tgagagcgct cccgtcgatg acctcggcgt cgggcggaat
    19921 cgcggtttcc gcgccgatgg actcgatgag cccgccgcgg atgatcacgg tcgccttggg
    19981 caggacgggt ccgcccggcg tcaccacgcg gcaatccttg acggcccaga cttcgtgcag
    20041 ggactcttcg gacgcgagga gcggaacgct cgcgagccag agacccaggc acaggaacag
    20101 ggaggccggg ggtaatctcc tcttggccat catgcctcct tctggcgggt ggaatagtcg
    20161 ttcaagcgtc ggaagggaac atcatccgga cgcgaacgtt gatcttgatt atatacgatt
    20221 gcggctggaa aaaggttcgt atggaaagga ccggaacacg ggatgttttg ccccgcggcc
    20281 gctccgcggg gaccccggcg taaatttgaa gcggcggacg ggcggttccg tttgacaagg
    20341 cccccctggg gttctatatt cgagaaaagc aaaaatcggc ggaattgcgg aggaacggcc
    20401 atgaagaaga ttttccggat cggggcggcc gccgccggcc ttttggttta cggggcggcc
    20461 ggaggccatg gtttttcgga tgttttcaag acggcggaag gggatttgaa aatctctctt
    20521 cccggccacg cgaccgtttt tttcgagttc ggggggacaa ccgtctgggt ggatccgact
    20581 aagccgtacg tctttgttcc cgaccatccg cccaaggccg acgtgatcct ggtttctcat
    20641 gagcacggcg atcatcttga tctcgagggg atcgaggccg tcaagaaaac agggacgagg
    20701 atcatctgtc cggccgtgtg cgccggcccg ctgcccggcg gcgaaatcct ggccaacggc
    20761 gggaaaacgg cggttctcgg cgtctccatc gaagccgtcc cggcttacaa cctcgccggg
    20821 atgcggagtc ccggcgtgcc gttccacccc aagggcgcgg gcaacggcta tgtgctgacc
    20881 ttcggcggct tgcgggtcta tgtggccggc gatacggaga acgttcccga ggtcaaggcc
    20941 ctgaagaaca tcgatgtcgc cgttctgccc ctgatgacgc cctacacgat gaccgaggag
    21001 atgctggccg acgcggcccg ggccttcaag ccggacgtcc tgtatgtcta ccatacccgg
    21061 accgaggatt tttcgaagct gagaaatctc ctgaaggatt ccggcatcga tctccgcctc
    21121 ccgtcctgac gtccgcgggg aaattttctg caacggggcg gagcggaaac gacccatcaa
    21181 aaccgggcgg acggcggtcc gtcgaaatct ttttccgccc gccggacctc ggcgacaaag
    21241 gcggcgacct tatcgaagtc ctttttgaac cggacgggtt tcccttcccg gtccagggcg
    21301 ttggtcaggg tgcagctgtc ggccccggcc ggacgaacgc ttcgcatgga ttcatagacg
    21361 ttctccggcg acaagccgcc ggccaggatg acgggaatcc ggcttgcccg gacgattttc
    21421 gcggcccggt ccaggtccgg acgccggccg gtgatgccca cgaagccctc gaccggctcc
    21481 cggcccagcc aggtgtcgat caggaaacca tcgctcgaat cctccagggc ggccgcgatt
    21541 tccagggcgg gaaagcccgg aagccggccc gcttccggaa ccggaagggt ccggacgatt
    21601 ccgattccag ggaatctttc cctgaaacga tgttgctttt cggcggcggg ccgcaggtcg
    21661 tgcggccggc cttgctcgtc ggtcaaggcc tcgcagaaat gaacataatc gggccggaga
    21721 atatccatca ccgaaaaaag cttgttttca ttggaaaaaa gcggaatcag ggagcttttc
    21781 gcgtctgttc cgcggacgag ctcgacgacc tcccggatcc ggctccgtcc ccggtccgtt
    21841 tccgaaagga tcacgcttcc gagacggtcc acgcccaggg cgaggcagcg ctccgcttcc
    21901 tcgggcgtct ggatttcata gatttggacg atcatgattt ttcggaaaaa gactatacgt
    21961 ttccgccggc gtggggtcaa tggggaaagc cccttgacat caggtttgct tttcgctagg
    22021 atacttccgc tgcttcgtat ccttttgctt ctacattaag aaaaaagtct ggaaagcagg
    22081 acttttttcg gaaagatgcg ccgctggcgg tgcgtctttt ttccttttgg gagaggtttt
    22141 tttgccagcg tagctcagct ggtagagcgg ctgatttgta atcagcgggt cgggggttcg
    22201 attcccttcg ctggcttagg agcatctgct gaggcggata aagcatgaaa gccgggcagg
    22261 taccaaagtg gttaaatggg acgggctgta aacccgttgc ggaaacgctt cgaaggttcg
    22321 aatccttccc tgcccagaat aacgacgcgg gaatagctca gttggtagag cggtggcctt
    22381 ccaagccatc tgtcgcgggt tcgaatcccg tttcccgctc ttaaggcatt tttcgtggcc
    22441 gcccacgtag ctcagttggt agagcacgtc cttggtaagg acggggtcac cagttcaact
    22501 ctggtcgtgg gctttttcga aatacaaccg agcagctcaa cgccggaacg ataggaggac
    22561 atgatggcaa aggcgaaatt cgagcggacg aagccccatg tgaacgtggg gacgatcggg
    22621 cacattgatc acgggaagac gacgttgacg gcggcgataa cgaaggtgct ggcgacgaaa
    22681 gggctggcgc aggcgaagaa ctacgacgat gtggcgaaag ccgacgcgaa gatgttccgt
    22741 cgggacgcga cgaagatcct gacggtggcc ctggcccacg tggaatacga gtcggagaag
    22801 cggcattacg cgcacatcga ctgtccgggg cacgccgatt acatcaagaa catgatctcg
    22861 ggggcggccc agatggacgg gtcgatcctg gtggtctcgg cggcggacgg cccgatgccg
    22921 cagacgcggg agcacatttt gctggcgcgg caggtgatgg tgccggcgat cgtggtattt
    22981 ttgaacaagt gcgacctggt ggacgacccg gagattctgg agctggtgga gctggaagtc
    23041 cgggagctgt tgacgaagta cgaatttccc ggggacacga cgccgatcat tcgggggtcg
    23101 gcgacgaagg cgatggaaga catcgagggg cccgggggga agccgatttg ggatttgatt
    23161 gaagcgatgg acacgttcat tccggagccg gcgcgggaag tggacaagcc gtttttgatg
    23221 agcgtggagg atgtgttcac gatcacgggc cgggggacgg tggtgacggg gcgggtggac
    23281 cgggggacgg tgaaggtggg cgacgaggtg gaaatcgtcg gattcacgga gacgcggaag
    23341 acggtggtga ccggggtgga gatgttccgg aagctgctgg accaggccca ggcgggggac
    23401 aacatcggtt gtctgctgcg ggggattggg aagacggacg tggagcgggg ccaggttttg
    23461 gccaagccgg ggtcgatcaa gccgcacaag cggtttaaag cggaagtggt ggccctgacg
    23521 aaggaagaag ggggccggca cacgccgttt ttcacggggt accggccgca gttctttttc
    23581 cggacgacgg acgtgacggg gaacgcgaag ctgccggagg gcgtggagat ggtgatgccc
    23641 ggggacaacg cgaacctgga gatcgacttg atttcggaag tggcgatgga gaaggggctg
    23701 cggttcgcca tccgcgaggg cggacggacc gtcggcgccg gaaccgtcac cgaaatcctg
    23761 gagtaagcgg gtcccatgcg agagatcatc accttggcct gcaccgcctg caagcggcgc
    23821 aattactcga acacgaggaa taaaaagaag cagacggacc ggctggagat cagcaagtac
    23881 tgtccgttct gccggaaaca cacttcgcac aaggaaacca agtaaggacg aacggcgatc
    23941 agaaaagaca ggtgagtagc tcaactggct agagcggcgg tctccaaaac cgcaggttgc
    24001 gggttcaagt cctgcctcac ctgccagttt tcttatcctg agagccggat ccgcggacaa
    24061 cgcccgaaga aggagcggcg gcaaacatga gcgacaagcc gagatggtac aagcgtttct
    24121 gggctttttt gaaggaagcc cgggcggaac tgaagaaagt gacctggccg tcgcgcgccg
    24181 aggtcataaa cacgacgatc gtggtgatca tcgccaccgt ctttttcggc ttttacttgt
    24241 tcttcatgga cgcgttcttc gcctgggcgc tcagcaagct gaaggcgctc atcggataac
    24301 ggagcggcga tggccaagaa ctggtacgtc atccacacct actcgggatt cgagaagttc
    24361 gtctcggaat ccatccgcca gcgggcggtg gaaaccggca tcgaggacca aatcggccaa
    24421 atcatcatcc cgaccgagga cgttatcgag atcaagatgg gcaagaaggt cgtctcgacg
    24481 aaacggtctt acccggggta catcctcatc gagatggaga tgaccgacga gaactggctc
    24541 atcatccgcg acacgcccaa ggtcacgggt ttcgtcggct cgggcaaaaa accctcgccg
    24601 ctcagcaaat cggacgtttc ccagatcgtc acccacatgg agacgacctc ggaaaagccc
    24661 aaacccaagc attccttcga aaaaggcgaa gcggtccgga tcatcgacgg acccttcttc
    24721 aatttcaacg gcatcgtcga ggacgtcaac catgaacgca gcacgctgaa ggtcctcgtc
    24781 acgattttcg gccgttccac gcccgtcgag ctcgaattcc tccaggtgga gaagctgtag
    24841 gaggcacctc atgtcgaaag aagtcgtggc ccgcatcaag ctgcaaatca cggccaagca
    24901 ggcgagtccc gcttcgccgg tcggccccgc cctgggccag cacggcgtca acatcatgca
    24961 gttcgtccgg gaattcaacg accggaccgg caagatggag gagggggtgg tcgttcccgt
    25021 cctcatcacc gtcttcaagg acaagacgtt caatttcatc atgcggaccc cgccggcgtc
    25081 ctacctttta aaaaaggccg ccgggatcgc caagggatcc ggggctccca acaaggacaa
    25141 ggtcggccaa gtgacccaga agcagatcga ggagatcgcc aaggtgaagc tgcccgacct
    25201 gaacgccaac gacgtcgccg cggcggtcaa gatcgtcgag ggcacggcaa aaaacatggg
    25261 attggagatc atccgtggct aaacgaggca agaagtatct caaggcgcgg gaactgagga
    25321 cgcggcccga gtactcgatt gacgaggccg tcaagctcct caagcagggc ggtttcgcca
    25381 aattcgacga atccgtggac gtggccatgc ggctgggcgt caatcccaag caatcggacc
    25441 agatggtccg ggggacggtc gtccttccgc acggcaccgg ccggtcgaag aagatttgcg
    25501 tcctggccct cggcgagaag gtcaaggaag ccgaggaggc cggggccgat tacgccggga
    25561 gcgaggagct gatcgagaag atcgccggcg gctggatcga tttcgacgtc gtcatcgcca
    25621 cgccggacat catgcggaac gtcggcaagc tcggccggac gctggggacg aagggcctga
    25681 tgcccaatcc caagtccggg acggtgacct tcgacgtcaa gaaggcggtc ctggagacca
    25741 aggccggcaa ggttgagttc cggatcgaca agacctcgat catcaatgcc tcgatcggca
    25801 aaatctcctt ccccgaggag aagctggcgg aaaacaccag ggccttcatg gccgccgtca
    25861 tccatgcccg gccggctacg gccaagggcc ggttcgtccg gagcatgtac ctgagctcga
    25921 cgatgggtcc gtcgtacaag atcaccgagg aaatcctgga aaagtaggaa cgtcgccatg
    25981 aagaaagaag tcaaagccca gcgcatcgac gagctgaagg aactgttcga gacgaacggc
    26041 acctattacc ttttcaacta caacaaaatg accgttgccc aggcgacggc cctgcgcaag
    26101 aacctccgga aacagggatc ggccttgaag atcgtcaaga accgcctggc cctccggtcg
    26161 gttcccggcc ccctggccga tgatctccgg ccggtgttct ccaagcccac ggccgtggcg
    26221 tacaccggcg gcgacgccgt cctcctggcc aaaaccctgc gggaattcca ggtccagaac
    26281 aaggtcctgg tcgtcaaggg cggggtcgtg gaagggacat acatcggctc ggaacggttc
    26341 gatgaaatcg tcaagctgcc cggccggatg gacctggtgg ccaaggtcgg cggcctcatg
    26401 gccgccccga tgtcggggtt cctgcggtgc ctccgggcgc ccctgggcga cttcggcatc
    26461 cttctgaacc agctcaaagc caagaaagaa tcggcataga gaatcaaagc ctgaggaaag
    26521 gagacacacg tgtctaaact caccaaggaa gaatttttca ctcatctcga cggcctgacg
    26581 gtcctggaac tggcggacta cgtcaaggag ttcgaaaccc gctacggcat cagcgccgcc
    26641 gcggccgtcc ccgtggccgc cgccgctccc gccgcggaag ccaaggccga ggaagccaag
    26701 accgaattcg ccgtggtcct caagaacgcc ggcgaaaaaa agatcaacgt gatcaagatc
    26761 gttcgggaga tcaccgccct cggactcaag gaagcgaagg atctggtcga cggcgcgccc
    26821 aagacggtca aggagggctt gtccaaggcc gatgccgaag ccatgaagaa aaaattcgaa
    26881 gaggtcggcg cccaggttga attgcagtaa gcacgcgatc gcccgccgcg atgaacgggc
    26941 tcgtgttccg ggacggcatc tgtctttggc ccaacgcttg ggagggaggc gtccatgatt
    27001 aaagaaagcg aacatctcta ccgcgaacga ctggacttct ccaaaatcgg acaggtcttt
    27061 cccatgcccg atcttttgga catccaggtg ggctcctaca aggagttcct gcagatggat
    27121 ctcctgcccg aggaaaggaa ggacaccggc cttcaagccg ccttcaagga cgtctttccg
    27181 atctccgact tcaaggagac cactcagctg gatttcatca gctacgggat cgggaattgg
    27241 gaatgcaagt gcggccggct caagggagtt gaaaactccc gggccaaatg cacgtcctgc
    27301 agcaccctct ttccccatgg caccgtcctg acggaaaagg aaatctgtcc cttctgcaac
    27361 gccgcccgca agatcgaggt cccggtctgc gaacactgcg gcgaccgggt caccctcaag
    27421 atgaagtaca gcccgaacga atgcctccag aagggctata ctttctcggt ccccctccgt
    27481 ctcaaggtcc gcctgatctc ctgggaaaag gatgcgacga cgaaggccaa gcgcctcaag
    27541 cacatcaagg aacaggaagt ctatttcggc gacatcccct tgatgaccga taagggaacg
    27601 ttcatcttca acggcatcga gcgggtcgtc gtcagccagc tccagcgctc cccgggcgtt
    27661 ttctaccgcc cgggcgaggt gaagggctac ttcatcgcca agatcattcc gtaccgcgga
    27721 gcctgggtcg agtttgaata cgacgtcaaa aacatcctgt acgtccggct cgaccggaaa
    27781 aagaagttcc tggccacggt cttcctccgg gcgctcggct tcggctccga tgaggagctg
    27841 atcaagctct tccacgccgt cgtcaagatc actccggaaa acggcaagct ctactgggag
    27901 ccggtcgatt ccctggccgg ccggacggcc gccgaggatg ttctccatcc gaagaacggc
    27961 aaggttctcg tcgagcgaaa gaagaagttc accctggaga acatcgccgt tttgaaggag
    28021 gccggagttc cccgggtcgc cgtggaccgc aaggatttcc agaacgccta cgccttatcc
    28081 ggaatcaagg gcaaagtcaa agcctgcgag gagatcggcg acacggccct cgacctcctg
    28141 atcggcaagg gagagccgtt cgagatcttt ttcccggccg aggacgcggc cgggatgatc
    28201 atcgtcaata cattgaaaaa ggatctccgc aaggacacca accaggcctt ggccgaaatc
    28261 taccgcaagc tccggcccgg cgagccgcac acgatggaga gcgccgcgaa tcttttccgc
    28321 agcctctttt tcaatcccca gaaatataat ttcagccgga tcggccgcct caagtttaac
    28381 atcaagctgg ggaaggaaac ctcattggag gagaaagtcc tccagccccg ggattatgtc
    28441 gacgtcatct cctatctcct caatctccgc cggggcgaag gggacgtgga cgacatcgac
    28501 cacctcggca accgccgggt tcgttcggtg ggcgagcttg tcgagaacgc cttccggatc
    28561 ggcctgaccc ggatggagcg gacgatcaag gaaaagatga cgatcaccct ggacctgccc
    28621 tcggccatgc cccaggacct catcaattcc aagcccgtca tcgccgccct gaaggaattc
    28681 ttcggatcct cgcagctgtc ccagtttatg gaccagatca acagcctgtc cgaggtgacc
    28741 cataagcggc gcctcagcgc cctcggtccc ggcggcttga gccgggagcg ggcgggcttc
    28801 gaggtccgcg acatccaatc ctcccattac ggccggatct gcccggtcga gacgccggag
    28861 ggcccgaaca tcggattgat ttcctctctc agctcctatg cccgggtcaa cgagtacggc
    28921 ttcatcgaaa ccccctaccg ccaggtcaag gacggccgga tcatcgatta cgtcaagatc
    28981 ctccatcccg gcgattccga gtacaagtat ggcgatatcg tcaagaagga cgacgtgggc
    29041 aaggcggtcg cctccctgaa gcggaagggc gcccgccatc ttcccgtcgt ccagccgaac
    29101 tgccactact tgaccgcctg ggaagaagag gaccacaata tcgcccaggc caacgcccgg
    29161 gtcgacgagg acggccgtct cgtcgacgag ctcgtctccg cccggcaggc gggcaacttc
    29221 aagctcctgc ctcgggagca gatcgactac atcgacgtct cccccaagca gctggtttcg
    29281 ctttccgccg ccctcgtccc tttcctggag cacgacgacg ccaaccgcgc ccttatgggc
    29341 tccaacatgc agcggcaggc cgtcccgctc ctgcggccgg tcgccccgct ggtgggcacg
    29401 ggcgtggagc atcttgtggc caagggttcg ggcgacgtca tcgtctgcaa gagatcggga
    29461 accatcgcct tcgtcgacgc cgagcggatt ctcgtccatg tcgacgaagc caacaagacc
    29521 aaggattacg aggtcgcgac cgacctctat gtcctgacca agttcctccg gaccaaccag
    29581 aatacctgct tcacccagcg gcccatcgtc cgcaagggcg acgcggtcgt caagggccag
    29641 gtcctggccg acagctcctg caccgacctg ggcgagctgg ccctcggccg gaacatcgtc
    29701 gccgccttca tgccctggcg gggttataac ttcgaggacg cgatcgtcgt ctccgagcgg
    29761 ctgatcaaga aggacgtctt cacctctctt cacattgtcg aggaaaccat cgaggcccgg
    29821 gacaccaagc tcggccccga ggaaatcacc cgggacatcc ctaacgtccc ggagaacgtc
    29881 ctccgcaacc tggatgagaa cggcatcgtc cggatcgggg ccaacgtcaa ggccggcgac
    29941 gtcctcgtgg ggaaagtcgc ccccaagggc gaaacccagc tttcgcccga ggagaagctt
    30001 ctgaaagcca tcttcgggga gaaagccctg gacgtcaaag atgcttctct ctattgcgcg
    30061 cccggcgtcg aggggacgat catcgatgtc cggatcttca gccgccgggg cgcggaaaaa
    30121 ggcgtccggg ccaagcagat cgagaaggac gagatcgccc ggatgaagcg aaacttggac
    30181 gacgagtgct cgatcctcga tagcgagcgg gagcgcaagg tcaaggccct tctcaagggc
    30241 accgtcgtcg agaaggagca gaagctcggc tccctgacga tcgccaaggg gcagaagctg
    30301 accgaacgga tcctggacga gatcgacctc tccgatatcg gcaagatcaa gctcgcggcc
    30361 gacgaggacc gggacaaccg gatcaaggac gtcgagcgga agatccgccg ccaggtcgag
    30421 gccctgaagg ccattttcaa ggaaaaagtc gacgccctga agaagggcga cgagctggct
    30481 ccgggcgtca tccaggccat caaggtctac atcgccatga agcggaagct gtcggtcgga
    30541 gacaaggttt ccggccgtca cgggaacaag ggcatcatcg cccggatcgt ggccgaggaa
    30601 gacatgcccc ggctgccgga cggcaccccc gtcgacgtcg ttctcaatcc cctgggcgtt
    30661 ccctcccgga tgaacgtcgg ccagatcttg gagatccacg ccggctgggc ggcccagaag
    30721 ctcaacctct ggctcgacac gcccgtcttc gacggggccc atgaggcgga ggtcaaggag
    30781 ctcctccgga aggccgatct tccggaatcc ggcaagtcga ttctctacga cggactgtcc
    30841 ggcgagccgt tcgaccagga aatcaccgtc ggcaacatgt acatgatgaa gctgttccac
    30901 ctggtcgacg acaaaatcca cgcccggtcc acggggccct attcgctcat cacccagcag
    30961 ccgctgggcg gcaaagccca gttcggcggc cagcgcttcg gggagatgga agtgtgggcg
    31021 ctggaagctt acggcgcggc ctataccctc caggagatcc tcacggtcaa gtccgacgac
    31081 gtcgaaggcc gggccaagat ctacgaagcc atcgtcaagg gcgatctgga attcactccg
    31141 ggtctgccgg agtcggtcaa cgtcctgatc cgcgagctgc aaagcctctg cctgaacgtc
    31201 gagctcggga aggacgaaaa acaggagatc cttcaggtct tgggaatcga tgccacgcat
    31261 atccttaaag gagagcgctg atggacatca agcagacgct ggggggcaag ccccggatgg
    31321 attttgattg ggtccgcatc tcggtcgctt ctccggagaa gatcaagagc tggtcttggg
    31381 gagaagtgac caagcccgag accatcaact accgaacctt caagccggag aaggacggcc
    31441 ttttctgcgc aaagatcttc ggcccgatca acgactacga gtgtctctgc ggcaagtaca
    31501 agcggatgaa gtaccgcggc atcatctgcg agcgctgcgg cgtcgaagtc accaagagca
    31561 aggtccgccg ggagcggatg ggccacatcc agctggcttc cccggtggct catatctggt
    31621 tcttcaagtc ccctccgagc cggatcggcc tggttgtgga cctttcgatc aaggatctcg
    31681 agaaggtcct ctacttcgag tcatacatcg tcctcagcgg cggagatacg ccgctcaagg
    31741 aaaaacagct cctgaacgag gaggagtaca aggaagcccg ggagaaatac ggcgacaagt
    31801 tcgaggcgtc catcggggcc gaggccatca aggttttgct cgaaaggatc gacatcaaga
    31861 aggaagccga ccgtctccgc aaggccatga agaaggagac gtcccagcag cggcgcctcc
    31921 ggtattccaa gcggctgcgg gtttttaaag ccttgaagaa gtccggcaac cggccggagt
    31981 ggatggtctt ggacgtcgtc ccggtcatcc cccccgacct gcggcccttg gtccggctgg
    32041 acggcgggcg gttcgccacg tccgatctca acgacctcta ccgccgggtc atcaaccgga
    32101 acaaccggct gaagaagctc atcgagctgc gggcccccga gctgatcatc cgcaatgaga
    32161 aacggatgct ccaggaggcg gtggacgcct tgttcgacaa cggaaagcgg ggccgggttc
    32221 accttggagc caaccgccgc cccctgaagt ccctgagcga atcgctccgg ggcaaacagg
    32281 gccgcttccg ccaaaatctg ctcggcaagc gggtcgacta ctccggccgt tcggtcatcg
    32341 tcgtcggccc cgagctcagg ctcaaccagt gcggcctgcc caaaaaaatg gccctggaac
    32401 tcttcaagcc gttcattttc cataagctgg agaaggaagg gctggtggcc agcatcaagg
    32461 tcgcccggga gtggcatgag caggagcgtc ccgaagtctg ggacttcctc gaggaagtcg
    32521 tccgcgaaca cccgatcttc ctcaaccggg ccccgaccct ccatcgcctc ggcattcagg
    32581 cctttgaccc gatccttgtc gaggggaagg ccatccagat ccatcccctc gtctgcgcgg
    32641 ccttcaacgc cgacttcgac ggcgaccaga tggccgttca tgttcccctc tccgtcgagg
    32701 cccagatcga ggcctcgacc ctgatgcttt cggccaacaa catcctctcc ccggccaacg
    32761 gccggccgtt gacgatcccc agccaggaca tggtcctcgg atgctattac gccaccatgg
    32821 ccaagaacgg acttcggggc gagggacgca ttttcagcaa cgcccacgag gttctcctgg
    32881 ccttggagga caagcagatc agccgccaga gcctgatcaa gatgcgctac accgggccgt
    32941 tcatgaacct gggcgtgtat tacgacgacc aagccgtcca tacctgcccg gtgacccgga
    33001 ttgagaaggg cttgatcgag acgaccgccg gccggatcat cttcaacagc gtgctcccta
    33061 agagcatgcc cttcttcaac ggaaccttca agaagaaggg gattgaaagc ctggtgttct
    33121 acatttacct ccggtgcggg ctgagggaaa cggtcgaaac cctggaccgg ctcaaggaac
    33181 tcggcttcct ccaggccacc caggccggat tttccctggg aatcgacgat ttcgtcgtcc
    33241 ccaaatccaa gagcgagctg gtggagaagg ccaacaaaaa gatccgcgag gtcgagaagc
    33301 tctacctcga cgggacgatt tccgcccgcg agcgctttaa cacgatcatc aacgtctggt
    33361 cgaacgtgac cgacgaggtc tccggggcca tgatctcgga aatgaagcgg atctccttcg
    33421 agacgcccaa tctcaacccc ctgttcgtca tggccgactc cggttcccga ggcaacaagc
    33481 agcagatccg ccagctggcc gggatgcggg gcctgatgtc caaaccctcg ggcgaaatcc
    33541 tggagacgcc gatcacctcc aaccttcgag aaggcctgaa cgtcatccag tacttcatct
    33601 cgacccacgg ggcccgcaag ggcttggccg acacggccct caagacggcc aactcgggat
    33661 acctgacccg caagctcgtc gacgtttccc aggacgtcat cgtcgaccag cacgactgcg
    33721 gaacgctcaa gggcgtcaat gtttcggcga tcgtcgaaaa cggcgaaatc gtcgagcctt
    33781 tcatcgaccg ggtcgtcggc cggacctcgc ttgaacgggt cctgcacccg gacacgggag
    33841 aggtcctggt cgatacgaac caggaaatca ccgaaaccat cgcccaggag atgcagaacc
    33901 tgggcatcga gcgggtgaaa atccgctcga tcctgacctg cgaggccaag cggggcgtct
    33961 gccagctctg ctacggccga aacatggctt ccggattcac cgtcgagctg ggagaagcgg
    34021 tcggcgtcgt cgccgcccaa tccatcggcg agccgggaac ccagctgacg atgcggacgt
    34081 ttcacgtcgg cggcatcgcc atgggcgaga aggaacaatc gaagatcgaa gccaagaacg
    34141 acgggacgct tcgcttccac ggcctccggg ccgtccaggg gccggagggc cacttcgtcg
    34201 tcgtcaaccg gacggccaac atcgcggtga tggattaccg cggccgcgag gtcgagcact
    34261 accaggtccc ctacggggcg aagcttcatc tgcccgacgg cggggcggtc aaagcccgcc
    34321 agctcttcgc tgaatgggat cccttcaacg ccctgatcct gaccgaagag ccggggctcg
    34381 tggtcttcca agacgtcgtc ccgaaggtca cgatggagga atcccaggac gagatcaccg
    34441 ggttaatgag ccagatcatc atcgaaccca aggacgagaa gcttcagccc cggatcgaga
    34501 tcgtcgatcc ccaagccaag gacaagcacg gcaagcccgt ccttctgaag cgctacttcc
    34561 tcccctcggg cgccaacctg gaggttcggg aaggcgtggc cgtggaggcg ggaaaaatcc
    34621 tggccaagat tccccgggaa caggctaaga ccaaggacat cacgggcggc ttgccgcggg
    34681 ccgaggagct tttcgaggcc cggcggccga agtcgccggc cgtcatctcc gaaatcgacg
    34741 gcgtcgttca tttcggcggc ctggtccggg gcttccgcaa gctgaccgtc aagagcgacc
    34801 ggggagtgga gaaggaatat ctcatcccca agggcgccca catcagcgtc ggagacggcg
    34861 agcggatcaa ggccgggtta tccctcatgg ccggcccggt caatcctcat gacatcctcc
    34921 gggttctggg cgagaaagag ctggccgagt acctcctccg ggaggtccag gaggtttacc
    34981 ggctccaagg cgtggccatc aacgacaagc acatcgaggt catcatccgg cagatgctcc
    35041 gctgggttaa agtcgaggaa gtcggggaca ccgattttct gatcgatgag caggtcgaca
    35101 agttcatgtt ccaggatgaa aaccagaagg tgatcgaacg gggcggaaag ccggccaagg
    35161 cccggcccct cctgctcggc atcaccaaga gcgctctgag cacggacagc ttcatcgccg
    35221 ccgcttcctt ccaggagacg acccgggtcc tgaccgaggc gtcgatctat ggaaagaccg
    35281 accgcctccg gagcctgaag gaaaacatca tcatgggccg cttgatcccc gccgggacgg
    35341 gctacaagta ctatcgcgat gtggagctgg cggaggatga aaagcctctc gaggcggagg
    35401 cccccctgga agaagcgccg gttccggaaa cctgaacaat cgtctccgga atcccggatt
    35461 ttccttgaca agggagggcg gaatttgtta gactccgtct actcgtttcg agtatcggtc
    35521 cgctcggatt tccaatataa cgagaaccga attttttaag taaggagtac tctcttgccg
    35581 acggtgagtc agctgattcg aaaaggacgc tcgatgaaag gcacgaagag caagtcgccc
    35641 gctcttgaac gctgccctca gaagcgcggc gtctgcaccc gggtcttcac cacgacgccc
    35701 aagaagccca actcggccct ccgcaaggtg gcccgtgtcc ggctgtccaa caacatcgag
    35761 gtgacgggtt atatccccgg gatcggacac aatctccaag agcactccat cgttttgatc
    35821 cgcggcggcc gggtcaagga ccttcccggc gtccgctacc atatcatccg cgggaccctc
    35881 gattccgagg gcacggccaa ccggttccag tcccgctcga agtacggggc caagcgtccc
    35941 aaggaaaagc gggccggttc gtaacgggga gaaatcgcca tgccacgccg cggaatcatc
    36001 aagaagcgca aacccctggg ggatcaaaag tacggctcca ccctcatcac caagttcatc
    36061 acgacggtga tgcagaaggg gaagcggagc acggctgaaa acatcgtcta cggggcgatg
    36121 gacatcgtcc aggagaaaac caaggaagac cccctgaaga tgtttgaaaa ggccatggac
    36181 aacgttcgtc cgatggtcga aacgaaatcg cgccgcgtcg gcggagcgac ctaccaggtc
    36241 ccggtcgagg tcgagctgaa ccgctcgctc tcggtcggcg cccgctggat cgttaaatac
    36301 gccgaggagc gcggcggaaa gacgatgaag gacaagctcg cgtcggagat cctcgacgcc
    36361 ttgggaaaca agggcggcgc ggtcaagaaa cgcgaggaca cgcacaaaat ggccgaagcc
    36421 aatagggcct ttgcccatta tcgttggtga tccgatacat ggtcgaaatt ctgcggttcg
    36481 gagaagccgg atgcccggaa aaagcgagga tggcgaatga cgcggacaac gccgctcgcc
    36541 gacacccgca atatcgggat cttggcccat atcgacgcgg gaaagacgac cacgacggaa
    36601 cggatcctct tcacgaccgg gatcacgtac aagatcgggg aagtggacga gggaacggcc
    36661 gtcatggatt ggatggctca agagcaggaa cgtggcataa cgatcacctc tgccgccacc
    36721 acctgttact ggaaccacca ccggatcaac atcatcgata cgcccggaca cgttgacttc
    36781 acggcggaag tcgagaggtc tctgcgcgtc ctggacggag cggtggtgat cctttgtgga
    36841 gtcggtggag tcgagcccca atccgaaact gtctggcggc aggcggataa gtaccgggtg
    36901 ccccggatcg cgttcatcaa taagatggac cgcgtcgggg cggatccgga gcacgccgtg
    36961 gcgatgatac gctcacggct cggcgcttat cccctcccca ttcaggtgcc gttgggacgg
    37021 gaggactcct ttcagggggt cgccgacctg gtggaaatgc aagtatatga ttggggtagg
    37081 caagtgaccg gaatggaatt tgaaaaacgg acgatcggag aggccgagga ggccgagttc
    37141 cgcgcccggc gggtcgagat gatcgaggcc ctggccgagg cggacgaaca gctcctcgag
    37201 gtctatctct ccggcgccga aatcccgccg gccgacatca aggccgccct ccgccgggcc
    37261 accctcgccg gccgatgcgt gccggttctc tacggagcct cgttccggaa caagggcgtt
    37321 caacccctat tgaacgccat cgtggattac ctcccctcgc cggccgacat cccgcccgtc
    37381 gagggtttcc acccaaaaac ccaccaccgc gaaatccgcc gcccctccga cgacgagccg
    37441 ttttcggccc tcgtcttcaa gcttcagaac gatcccttcc tcggcgccct ggcctatttc
    37501 cgggtttatt ccggaaaagc caaggccggg acggcctttt acaactccaa caaggacgac
    37561 gaggagcgcc ccggcaagtt cctggagatg cactccaaca agcgccggga ggtcaaggag
    37621 ctcttcaccg gcgacatcgc ggccatgggg acgatgaagc ccgtctccac gggcgatacg
    37681 ttttgcgcca agaaccggcc cgtcatcctc gaggcgatcc gtttcccgga gccggtcatc
    37741 tcggccgtcc tggagccgcg cacccgggcc gaacacgtca agctccaaac ggccctggcc
    37801 aagctaacca aggaggatcc gaccctccgc gtcacccagg atcccaacac ggggcaaacc
    37861 cttatttacg gcatgggcga gcttcacttg gagattctga cggaccggct gtcccgggag
    37921 ttcggcctcc gggtcaacat gggccggccc caggtcgcct acaaggaaac catccttcgg
    37981 gcggccgagg gcgagggcgt ggttgaccgg ccgatcgccg gaaagcccca atacgcccgg
    38041 atccgcctgg ccgtcgagcc gttgggacgg caagccggct tcgtttttga gaaccggctg
    38101 aaaagcggga tcctgccgga cgtttacgcc gccccggtcg aggacggcgt ccgggaagcg
    38161 ctggacttcg gccagctggc cggctttccg atgaccgacc tcaaggtgac tctgaaggaa
    38221 gccgaccacc gggaggacga gacgaccgaa gcggccttca agatcgccgc ctccgcggct
    38281 ttcaagacgg ccgcggtcaa ggccgtcccc gtgctgctgg aacccatcgc ccggctggag
    38341 gtcatcgccc ccgaagaata cctgggggac gtcgtcggcg acctcaacgc ccggcgcggg
    38401 gcgatcgaca acgtggaggc gcggtccggc gcccgggtca tccacgccct ggcgccgctt
    38461 gcggagatgt tcggatatgc cacggttctg cggaccctga cccagggccg cggcgtgttc
    38521 acgatggaat tccatgccta cgaagccgtt ccgccccaga tcatggagca gatcgtcgcc
    38581 cggatcgagg gacgggtttc ctacgataat gaagggagga catgatggca aaggcgaaat
    38641 tcgagcggac gaagccccat gtgaacgtgg ggacgatcgg gcacattgat cacgggaaga
    38701 cgacgttgac ggcggcgata acgaaggtgc tggcgacgaa agggctggcg caggcgaaga
    38761 actacgacga tgtggcgaaa gccgacgcga agatgttccg tcgggacgcg acgaagatcc
    38821 tgacggtggc cctggcccac gtggaatacg agtcggagaa gcggcattac gcgcacatcg
    38881 actgtccggg gcacgccgat tacatcaaga acatgatctc gggggcggcc cagatggacg
    38941 ggtcgatcct ggtggtctcg gcggcggacg gcccgatgcc gcagacgcgg gagcacattt
    39001 tgctggcgcg gcaggtgatg gtgccggcga tcgtggtatt tttgaacaag tgcgacctgg
    39061 tggacgaccc ggagattctg gagctggtgg agctggaagt ccgggagctg ttgacgaagt
    39121 acgaatttcc cggggacacg acgccgatca ttcgggggtc ggcgacgaag gcgatggaag
    39181 acatcgaggg gcccgggggg aagccgattt gggatttgat tgaagcgatg gacacgttca
    39241 ttccggagcc ggcgcgggaa gtggacaagc cgtttttgat gagcgtggag gatgtgttca
    39301 cgatcacggg ccgggggacg gtggtgacgg ggcgggtgga ccgggggacg gtgaaggtgg
    39361 gcgacgaggt ggaaatcgtc ggattcacgg agacgcggaa gacggtggtg accggggtgg
    39421 agatgttccg gaagctgctg gaccaggccc aggcggggga caacatcggt tgtctgctgc
    39481 gggggattgg gaagacggac gtggagcggg gccaggtttt ggccaagccg gggtcgatca
    39541 agccgcacaa gcggtttaaa gcggaagtgg tggccctgac gaaggaagaa gggggccggc
    39601 acacgccgtt tttcacgggg taccggccgc agttcttttt ccggacgacg gacgtgacgg
    39661 ggaacgcgaa gctgccggag ggcgtggaga tggtgatgcc cggggacaac gcgaacctgg
    39721 agatcgactt gatttcggaa gtggcgatgg agaaggggct gcggttcgcc atccgcgagg
    39781 gcggacggac cgtcggcgcc ggaaccgtca ccgaaatcct ggagtaagcg aacgaacccg
    39841 gacgccggcc cggaaggggc ggcggcggga ggagataacg aaggaccgaa catcatggaa
    39901 aaaatcagaa ttcgattgaa atccttcgac catcggctgc tcgaccagtc ggtgaaggac
    39961 atcatcatca aggccaagcg gaccggcgcc cagatcgcgg gcccgatccc gctgccgacg
    40021 cggatccacc ggttttgcgt cttgacgtcg ccgcacgtgg acaagaaggc ccgggagcac
    40081 ttcgaaatgc gggtccacaa gcgtctcctc gacatcagcg agtacaacaa cgagacgatc
    40141 gaggagctgc agaagctgga cctgcccgcg ggcatcgacg tcgagatcca ggcctatgga
    40201 gcgggggagt gagccatgcg ggatggactg atcggtaaaa agatcgggat gacccagggc
    40261 tacgacgccg ccggcaattc cctgcctctc acggtcctca aggccggccc gtgcaccgtc
    40321 atccagagaa agacgacgaa gcgcgacggt tacgaggcca tccagctggc cttgatcgag
    40381 gaccggccgg cccgcaagcc cggcaaggcc ctccagggac attacgtcaa gtcgggcgcc
    40441 ccggtgatga aggtcctccg ggaagtccgc tgtgacggcg aaaccccgct caaggaaggc
    40501 gaccagatct tcgtcgacgt cttcaaggcc ggcgagaagg tggccgtcac cggcgtctcc
    40561 aagggaaagg ggttcgccgg ggtcatgaag cggcacaact tcggaggagg cgccggggcc
    40621 cacggctcga tgttccaccg ggctccgggg tccatcggcg cgtcctccta tccctcccgg
    40681 gtcatcaagg gcctccggat ggccggccgg atgggcggtg aacgagtcac cgtccgcaac
    40741 ctggtcgtct tcgaggcgga caaggacaac aacatcctcg tcgtcaaggg cgccgttccc
    40801 ggccccaacg gcgggtatgt tttgatccgg aaaatataag ggctcgtcat gaagaaagcg
    40861 aaagtcatcg attcccaagg cgccgtcaag gccgagatcg acctgccgga aagcgtcttc
    40921 ggctatccgg ccaagggaca tctcgtgtac gaggcggccg ttaattacct ggccaatcag
    40981 cgccggggca cggcctcgac caagacccgg gccgaggtca aaggatccaa ccgcaagccc
    41041 tggaagcaga aaggcacggg ccgggcccgg gccggggcca cccgcaatcc gctctggcgg
    41101 aaaggcggaa cgacgttcgg gcccaagccc cgcgattact cctactccct gtccaaggaa
    41161 tccaagcgca acgccctgcg ggcggcgctc tcggccaagc aggccgccgg acgcattctc
    41221 atccttgaga acctggccct gtccgcgccc aaaaccaagg aaggggcgtc cctgttgaag
    41281 gccttgaagg tctcgtcggc cctggtcgtc gacctcggcg cgaacaagaa cctgttcatc
    41341 gccctccgga atattcccaa ggtcaagccg gtcgaaccgt cccgggtcaa cacccacgac
    41401 gtcgtcggcc acgagtggtt cgtggccgga cgggacgcgt tcacggccct tatggagaga
    41461 ctccagtgag caaggatccg acccagatca tcctccgtcc cgtcatcacg gaaaagagca
    41521 cgctcctcag ggagcggaac cgcgaagtct gcttcgccgt cgcccggacc gccaacaagt
    41581 ccgagatcaa gaaggcggtc gaacacctct tcaaggtgaa agtggacacc gtccggacgc
    41641 agatccggcc gggcaaggag cggcgcctcg gccggagctc gggataccgg cccgattgga
    41701 aaaaagcctt cgtccgcttg aaggaaggcg agaaaatgat cgaatacttc gagggagcct
    41761 gacatgggca tcaaatcgta ccgacccatc tcggccgggc agcggcataa aaccggtctt
    41821 gcctacgagg aactgaccgg cgaccgtccg cacaagcctc tcctggaatc cctgcccaag
    41881 aagggcgggc gcgacaaccg gggccgcctg acgatgcgcc accggggcgg cggcaacaag
    41941 acgatgtacc gcctcatcga cttccgccgc gacaaactcg acgtccccgg cgtcgtcgag
    42001 accgtggaat acgacccgaa ccggtcctcc ttcatttccc tgatcaagta ccgggacggc
    42061 gagcggcgct atatcctgac ccctctggag atgaaggtcg gccagaccat catctcttcg
    42121 gaccgggtcg tggatgtcct cccgggaaac gcgaccctgc tgcggaacat ccccctaggc
    42181 acggtcattc acaatatcga gatccacaag gaccggggcg gccagatgtg ccggacggcc
    42241 ggttcgggcg cccagatcct ggccaaggaa ggggcgtttg cccaggtccg gctgccgtcc
    42301 agcgaaatcc gcaagatcca cttggattgc cgggcggtca tcggccaagt cggcaacctg
    42361 gaccaccaga atatcagcgt cggcaaggcc ggccgcagcc gctggatggg ctggcggccc
    42421 cacgtccggg gaacggccat gaatccgatc gaccatcccc acgggggagg cgagggcaag
    42481 gccaagggcg gacgccatcc ggtcagcccg acgggcatcc cgacgaaggg atacaagacc
    42541 cggaagaaca agcggacgca gatcttcatc gtccggagaa ggagcaaata accatgggcc
    42601 gttcgctcgc taaaggaccg ttcatcgaca gccatctcgt ggagaagatc gaggtccagg
    42661 ccggacagac caagcaacgg accgtgatcc ggacctggtc ccgccgctcg accgtcatcc
    42721 ccgaaatggt cggcatgacc atcgccgttc acaacggccg caagttcatc cccgtgttca
    42781 tcaccgagaa catggtcggc caccggctgg gggaattctc gcccactcgg acgttcaagg
    42841 gccacaccac gaaggcggcc aaagcggtga aggtgggagc ctgacatgaa cgcccccaag
    42901 tacatcgcct cggcctcgac ccgccatgtc cggctttcga ctcagaaagc ccggctggtc
    42961 gccgacctgg tccggaagcg cctggtcggt gaagccttga cgatcctccg gtacaacgcc
    43021 aaggccaagc ccgcccaagt cctggagaag accctccgct cggcgatcgc caacgcccag
    43081 cagaagaacg ccggcctgga tgtcgacaac ctggtcatcg gcaagatcgt cgtcaaccag
    43141 ggaccggcct ggaagcggat ccgtccggcc ccccagggcc gggcctaccg gattcagaaa
    43201 cagacgagcc acgtgcacgt ctaccttgaa gaaaggacag ggaggtaacc cgtgggacag
    43261 aaaacgcatc cgcacggctt ccggctcggg ttcaacaagc agtggagctc ccgctggttc
    43321 gccaaaggcc ccgaatacgc ccgtctcatc catgaagacc tccggatgaa acaggagatc
    43381 aagaaaaaat acgcccacgc cggcatttcc gaagtggccg tcgagcgggt cggcccgaag
    43441 atccgggtcc tcattttcac cgcccggccc gggatcatca tcggccgggg cggcaaggaa
    43501 atcgaaagcc tgaagcatca cctggagagc atcgtcaagc gcgaagtcta cgtcgacatc
    43561 cgcgaagtcg agaagcccga gctcgtcgcc ctgctggtcg ccgagggcat cgccgtccag
    43621 ctggaaaagc gcatcgccta ccgccgggcc atgcgcaagg cggtcgagat ggccctgaag
    43681 atgggagcca aggggatcaa ggtgatgtgc gccggccgcc tgggcggcgt tgaaatcgcc
    43741 cgctcggagt ggtacctgcg cggccagctg ccgctccaaa cgctcaaggc tgacatcgat
    43801 tacggcttca ccgaggcttt caccacctac ggccagatcg gaatcaaggt ctggatttac
    43861 cggggcgata cggaaaaagc caagatgacg tcgcagacgg cggaggtcga ggagatctga
    43921 ctatgttgat gccgaagaaa gtcaagtacc ggaagcaaag ccgcggccgg atgtccgggc
    43981 tggccgaccg gggggcgacc ctctcgttcg gacagtacgg gctccaggcc tgcgaatgtt
    44041 gctggctcac cgcccgccag ctcgaggccg gacggatcac gatcacccgg ttcatgaagc
    44101 ggcgcggaaa gctgtgggtg cgctcgttcc cctggaagcc ggtcaccaaa aagccgaccg
    44161 aggtccgcat gggcaagggc aagggcgacc cggaattctg ggtagacgtc atcaagcccg
    44221 gacgggtcgt ctacgaggtg gaaggtgtgc ccgaggacgt ggcccgcgag gcgatgcgcc
    44281 tggcggctca gaagctcccg atgaagacgc gattcgtctc ccgggaaaac agggaactcc
    44341 gatgaaactc agcgaactcc gagaattgtc caaggacgag ctcctggtga aaaaagccga
    44401 gctcaaagac cagatcttca agctccgctt ccagcactcg ctgggccagc tcgaatcgac
    44461 ggcgaagctg cggaacgtcc gcagggagat cgcccggatc gagactctgg ccggcgaaaa
    44521 ggaccggaag gcgaaggcgg aataggaaac ggccatgaat tccaaagaac agactccctc
    44581 gcggaagacg accaaggtgg ggacggtcat cggggccaag atggccaaga ccgtctccgt
    44641 gcaggtcgag cggcagtacc gccatccgct ctacaaaaaa atcgtccgga cccggcgcat
    44701 cttcctggct catgacgagc acgggaagtg caaggtcggg gacgtggtcc gcattgtcga
    44761 gacgcgcccc ctcagccgcc acaagcgctg gcgcgtcgtc gaaatcgtcg ggctcgttcc
    44821 cccgagcgtc gaaacggatc tcggtgaggc ccagtcatga tccagcaata cacggttctc
    44881 aaggtcgcgg acaactccgg ggcccgccgt ctccggacga tcactccgct gggcggcggc
    44941 accggtctcg tctgctccat cggcgacatc gtctccgcct ccgtccgggc ggccgacccg
    45001 gaaagcaagg tcgagaaggg caaagtggtc cgggcggtcg tcgttcgggc ccgcaaggag
    45061 atccgccgca aggacggatc ctacatccgc ttcgacgaca acgccgcggt catcatcgac
    45121 aagcagggcg aacccgtcgg gacccgcgtc ttcgggccgg tcggccgcga actgcgcgaa
    45181 aagaaattca tgaaaatcgt ctcgctggcc ccggaggtga tctgatgagc gtcccgttca
    45241 tccggaaaaa cgaccaggtc atcgtccgcc agggcaagga taagggaaag accggcaagg
    45301 tcctttccgt cgatccggac aagggccggg ccctcgtcga gggcgtggcc atggccaagc
    45361 atttcatccg gcccgaccgg tccaagaacg ttcagggcgg aatcatggag aaggaatccc
    45421 ggattcccct gagccgcttg atgcttttct gccccgactg cgcggaaggc gtccggatcc
    45481 ggtcgaaaaa gctccaggac ggcacgcgga cccgcgtctg cgtcaagtgc gggacggttt
    45541 tggaaccggg aaagtgagga acccatgagc cgcctgctgg aacgctataa aaaagagatc
    45601 ctggccgagc tggagaagga gctggacatc gccaatccca tggccgttcc ccggctggaa
    45661 aagatcgtca tcaacgtcgg ggtcggcgaa gccatccaga acgccaaggc cctcgacgcg
    45721 gccaagctcg agctgaccca gatcacggga caggccccgg tggtttcccg ggccaagaag
    45781 tcgatttcgg ctttcaagct ccgcaagggc ctgccgatcg cctgcttcgt caccctccgc
    45841 cagcggcgga tgtacgaatt cttcgaccgg ctggtcaaca tcgtcctgcc gcgcgtccgc
    45901 gatttccggg gcgtgtcgcc caaggccttc gacggccggg gcaactacac cctcgggctg
    45961 cgggaccagc tcgtcttccc cgagatcgat tacaccaagg tggaacggcc ccgggggatg
    46021 aatatcacca tcgtcacgac ggcccggacc gacgccgagg ctcaggcctt gctcaagaag
    46081 ctgggaatgc ccttccgcga gacggcttaa gagaagagag gttattcatg tctaccacgt
    46141 ccgcttacgc caaatacctt cggaccccca agtacgccat ccggacgcgg cggcgctgca
    46201 agatctgcgg ccgctcccgg ggctattacc ggaagttcga gatgtgccgc ctgtgtttcc
    46261 gggacctggc ccacaagggc gagatcccgg gcatcgtcaa ggcctcctgg tgaggggccg
    46321 gaaaggaaaa gtacgatgag cgccacggat cccatcgccg atatgctcac ccggatgcgc
    46381 aacgccgctc tcatgcggag acgggaagtg cagatccccg cctccaagct gaggacggag
    46441 atcgcccgga tcctcaagga agaaggcttc atccgcaatt acaaggtcat caaggacggt
    46501 ccccagggaa tcctggccgt cacgctgaaa tacaccgagg gcaaccagag cgtcctggcc
    46561 ggcgcccggc gcatctccaa gcccggctgc cgggtctact gcacaaagga cacgatcccg
    46621 aaggtttaca acgggctcgg cttggccgtc atcagcacct ccaagggcat cctgacgggc
    46681 gccaaatgcg aggagcttgg gctgggcgga gaggtgctct gcaccgtctg gtgagaggcc
    46741 cgaaagaaag ggacacgcca tgtcacgcat aggcaaacgg tcgatcatct tgcccgcggg
    46801 cgtcaaggcc gcggttttcg ccgatcggat cgagatcgag ggcaagaaag gcaagctcac
    46861 gactcctctc tacgagggga tcacggcgaa cctggagggg acgaccctct ccttcgtccg
    46921 gtccgacgac accaagcagc gaaggatgtt ccacgggctt tgccgggccc tggctcaaaa
    46981 cgccgtcatc ggcgtttcag ccgggttcca gaagcagctg gaaatcgtcg gcgtcgggta
    47041 caaggcccgg gtcgaccgca accatctcga gctgagcgtg ggttattcca aaccgaagct
    47101 gtatccgatc cctcccgacg tcgaggtcgt gtgcgagaag ccgactctca tcagcgtccg
    47161 ggggatcgac cggcagcggg tcggccaggt cgcggacgac atccaccggt tccgcaagcc
    47221 cgatccgtac aagctcaagg gggtgcgcta tgtcggagag aagctcatta agaaagagcg
    47281 gaaggcagga gtgaccggtg cataaagaca agatcaaaat cagagcggaa cgggacgagc
    47341 gcctgcgccg gagaatccgc cgcaagatcc agggaacggc cgaccggccc cgggtgttcg
    47401 tcttcaagag caaccggtac atctatacgc aggcggtcaa cgatgagacc ggatcggtca
    47461 tcgcttccgc cagcaccctg gagaaggacc tccgggccgc gctcaagaac accaagaacc
    47521 tggccggctg cgagaagctg ggcgaggttc tggcccagcg gctcaaggcc cgcaatatcg
    47581 acaaggtcgt cttcgaccgg ggcgtctacc cctaccacgg ccgggtcaag gccgtcgccg
    47641 aagccatgcg gaagggcggc ttggcctttt aaggccggcg cgaaggagaa tcacaggtgc
    47701 aaactgaatc gacacgcaaa gaagggaacc ggcctccccg gccccggctg gacgatggaa
    47761 ccgagctcaa ggaccaggtc atcaccatcc gccgggtgac gaaggtcgtc aagggcggca
    47821 agaacctgag cttcgcggcc ctggtggcca tcggtgacga gcgcggccaa atcggcatcg
    47881 gcaagggcaa ggcccgcgag gttccctcgg ccatcgccaa ggccgtggag cacgccaaga
    47941 aacacatgat caggatccag ctggccgaca cgaccatccc ccatatgatc cggggagagg
    48001 ccgacgccgg cgtcgtgatt ctccgccccg cttccaaggg aacgggcgtg atcgccggcg
    48061 gcgccgtccg gatcatcctg gagctggccg gagtcaagga catcctgacc aagtctctcc
    48121 gcagcccgaa tccgatcagc gtcgcccacg cgacgatgga cgccttgatg aagcttaaga
    48181 atccggaaca ggtcgcccgg gatcggggca aggggaaagg accggtcgag aaatgaaagc
    48241 cgccgcctct aagaaaatca agatcaccct ggtccgcagc ttgatcagcc gcccggaaag
    48301 ccagcgcaag gtcgcccggg ggctggggct gggccggctg aaatcctcgg tcgtccgcga
    48361 ggacacgccg tccatccgcg gcatgatccg gaaaatcaac catgtggtga aagtggaggg
    48421 cgtcgaatga acctcagtca actccaaccc gccaaagggg ctcggaaaaa agaccggcgg
    48481 gtcgggcgcg ggcccggctc gggccggggc aagacctcgg gacgcggcca taagggccag
    48541 ttgtccggag cgggctacaa ggccatgcgc ggattcgagg gcggtcagat gccgctcgtc
    48601 cgccggattc cgaagcgggg cttcaccaat attttccgcc atgagccggc cgtcctcaac
    48661 ctcgaccgcc tggccaagat ctccggagcc gagataggtc cggccgaaat ggccgccgcc
    48721 ggggctctga aaccggcatc cgcccgggtc aaaatcctcg gccgcggcgg aatcgccgag
    48781 gccaagacga tccgggctca ttccttctcg gccgcggccg tgaaaaaaat cgaagcggcc
    48841 gggggcaagg ccgtcgtgat tgagtaaggc catgctggaa agcatccgca atatcttcag
    48901 cattccggac ctccggaaga gggttttctt caccctgatc ctgctggcga tttaccggat
    48961 cggcgggcag atcccgaacc cgggcatcag cgcctcggcc ctggccgagt tctggaacgc
    49021 ccagaagggc acgatcctgg gattcgtcga cctgttctcg ggccagtaca tgtcgaagat
    49081 gacgattttc gccctgggga tcatgcccta catcagctcc tcgatcatcc ttcagctgct
    49141 gaccgtcgtc tggccctatc tcgagaggct gtccaaggaa ggggagctgg ggcggaagaa
    49201 gatcacccag tacacccggt acggcacgat tctcatctgc cttgtccagt ccatcgggat
    49261 cgcctacttc ctccagaccc tgaagacgaa caccggccag ccggtcgtcc ccaacccggg
    49321 cctcggcttt atcttcctga cggttctgac cctgaccacg ggaacggtct tcgtcatgtg
    49381 gctcggcgaa cagatttccg agcgggggat cggcaacggc atctcgctga tcatctttgc
    49441 cggcattgtc gcccggtttc cggccggcct gaacagcatc atcagcggcc tgaagagcgg
    49501 cgactggaat ccgatcaaga tcatcctcct caccgcgatg atggtctcgg tcatcgcctt
    49561 catcgtcttt gtcgagcggg gccagcgccg catcccggtg tcctatgcca agcgggtcgt
    49621 cggccggaag gtcttcgggg gacaaagcac tcacctgccg ctccgggtca acaccggcgg
    49681 cgtcatcccg atcatcttcg ccgcctcgat catcaccatc ccggcgacga tcgccgcctt
    49741 gatcaaaacc ccggccttcc agacgttcgc ccggtggttc aacgtcggca tgccgcttta
    49801 taacctcctg tatgtcgcgg cgatcatctt cttcacctat ttctacgttt cgatcatctt
    49861 caacccgaat gacgtggccg agaacctccg caagtacggc ggattcatcc cgggcattcg
    49921 tccggggaag aacacgtcgg attacatcga cgagatcctc tcccggatca cgctcgtcgg
    49981 agcggtctac ctggccctct tggccatcct gccggagttc ctgacgaccg ggttcaagat
    50041 ccagtacctg ccgttcgtcg gccgtttcct ggagcggagc ctgccgagct ggatcatgaa
    50101 cggcttgggc gtcaacttct atttcggcgg gacatccatt ctgatcgtgg tcggcgtggc
    50161 catggacacc ctgcagcaga tcgaggctca gctcgtgatg cgtcattacg acggattcat
    50221 gcgccgcggc cggatccggg ggagaagggg atgagagtga ttctcctcgg cgcgccggga
    50281 agcgggaaag gaaccctggg ggagagaatc tcccgggcga ctggatttcc ccgcatctcg
    50341 accggcgacc ttctccggaa ggccgtccgg gagggaaccc cggtgggccg caaggccgag
    50401 gcagtcatga aggccggcgg gctcgtggat gacgcgaccg tcaccgggct tgtccgcgac
    50461 cggatcgccg ccccggactg tctgggggga tatattctgg acggtttccc ccggaccgtc
    50521 gcccaggcgg aggcggtcga ggcgatcgat ccggcccggc cggaagtcgt cctcgacctg
    50581 ctcgtgagcg aggacgtcat cgtagcccgg ctggacgggc ggcggatttg cgaagcatgc
    50641 ggagacgtgt atcatatcgt caataagcct ccgaaaaccc cgagcgtctg cgacgcctgc
    50701 ggcggagatc tcgtccagcg gcccgacgac gcggccgcga ccgtccggga gcgtctgcgc
    50761 gtctaccgcg aggcgacggc cccgcttcag gcccggtacc gggccaaggg gacgtaccgg
    50821 ccggtggacg gcgacggctt tcccgaggac gtgtattccc gggcggccgg cgtcctggcc
    50881 ggggagggat gccggctgtg atcgtctaca agaccgaagc cgagatcgcc cggatgcggg
    50941 agagctcccg gatcaccggg ctgatcctgg ccgaactcga gaccctgatc gcccccggcg
    51001 tgcggaccca ggatctggac gcccacgccg accggaggtc ccgggagttg ggagcccgcc
    51061 ccgccttcaa gggatatcgg ggctttcccg gcgccctttg cgtctcgatc aacgaggaga
    51121 tcatccacgg attcccctcg tcccggcgac tccgggaggg ggacatcgtc agcctggatt
    51181 tcggcgtttt ttacgacgga tactacggcg atgccgcccg gacgtttccc gtcgggcggg
    51241 tggagccgga cgccctgcgt ctgatccgga tcgccgagga gtcatttgag agcggtcttt
    51301 tgcgcttcgt cgagggcaac catctctcgg atgtctccca cgcgatccag tcggccgtcg
    51361 aggcggccgg cttctcggtg acccgttcgt ttgtcggcca cggcatcggc caggagctcc
    51421 acgaggatcc acagctcccg aacttcggct ttcccggccg gggtccccgg atccgcaagg
    51481 gcctcgtcct ggccgtcgag ccgatgatcg ccgccggccg ctgggaagtg gacatcctgg
    51541 gcgacggctg gacggccgtc acccgggacc gcagcctggc ggcccatttc gaagagaccg
    51601 tcgccctgac cgaccggggc gccgaggtcc tgagccgagt cggaacgaag gccgtggagc
    51661 cggcccggaa ggagttgccg tatgcctaaa gaagacgtct tcgaagccga aggcgtggtt
    51721 cgcgaaacgc ttcccaacgc gatgttccgc gtggagctgc agaacaagca catcatttta
    51781 gcccatattt cgggcaagat gagaaaacat ttcatccgga tcctgcccgg ggaccgggtc
    51841 ctggtcgaga tttcgcccta cgacctgacc aagggacgga tcacgtaccg atttaaataa
    51901 aggggagacg acatgaaagt ccgagcctcc gtcaagaaaa tctgccgcaa ctgcaaggtc
    51961 atccggcgca agggattggt ccggatcatc tgcacgaatc cccggcataa acaacgtcaa
    52021 ggataaggac ggcacccatg gcacgcattg caggcatcac gcttcctccg gacaagcgga
    52081 tcgaaatcgg gctgacctac atcttcggga tcggccggtc caagtcgagc cagatcctcc
    52141 aggaagccaa gatcgacaag aacaagaagg tcaaggccct gagcgaggag gaagtcaaca
    52201 agatccgcca gatcatcgaa aagagggaga agatcgaggg cgacctccgc aaggacatca
    52261 gcctttcgat caagcgcctg atcgatatcg ggtgctaccg gggccagcgg cataaaagga
    52321 aactcccggt ccgcggccag cggacgaaaa cgaacgcccg gacccggaag gggcctcgcg
    52381 gccacacgat caagaaattg aagccggccg ccaaatcata aggaaggaag cagcgcatgg
    52441 ccacgaaagc gaagacacgg accaagaaaa aagtccgcaa ggacatcggc tacggaacgg
    52501 cccacatcca gtccacgttc aacaacacga tcatcacgat ctccgatcac gcgggcaacg
    52561 cggtgtgttg gacgagctcc gggactgtgg gcttcaaggg cgcccggaaa ggaacgccgt
    52621 tcgcggccca gctcgccggc aaggaagccg cgacgaaagc ccgggaattc ggcgtccgtt
    52681 acctggacgt ccgggtcagc ggcccggggg ccggccgcga gtcggccatc cggtccctgc
    52741 aaagcggcgg ggtggaaatc cgttcgatca aggacgtcac cccgattccc cataacggct
    52801 gccgcgtccg ccgccgccgc agagtctaag gagagtcctc atgtcccgat atcgcgaacc
    52861 cttgtgccgg ctttgccggg tcgaaaaaac caagctcttt ctcaaggggc acaagtgcct
    52921 gaccgataag tgcccggtcg agcgccgggc ctacgccccc ggccagcacg gccgaagccg
    52981 ccggcgcatc ctcggctacg gcctccagct ccgcgagaag cagaagatga aacggttcta
    53041 cgggatgtcc gaaaagcagt tccgcctgtt cttcgagcgg gccgaacgga ccaagggcat
    53101 caccggcgag atcctcctcc agatgctcga gcggcgcttg gacaaccttc tgtacatcgc
    53161 cggctacgcc cattcccggg ctcacgcccg ccagctggtg acccacgggc acgtcctagt
    53221 caacagcggc cgggtcgcca ttccctcgtt catggtcaag gccaacgatg aaatcggctt
    53281 ccggccccaa tcggtgaaat ccgaggacct gaaggccgtg gccgaagcgt acgccggcaa
    53341 gaccgccccc ggctggatcc aggtcaaccg ggaaaacctc accgcccgcg tcatggccct
    53401 gccgacgcgc gccgacgtga ccctcccggt cgaggagcac atggtcgtcg agctctactc
    53461 gaagtagggc ccggctcgat aacggtttcc ggaaggagga cccatgaccg aatttggttt
    53521 tcagcggccg aagctattgg aatgcgatta tgagaccttg acgtcgacgt acggccggtt
    53581 cacggcccag ccgttcgagc gcgggtacgg gacgaccgtg ggcaacgcct tgcggcggat
    53641 tctgctgtcc tcgatcaagg gcgcggccat caccgccgtc cggatccagg gcgtgctcca
    53701 tgaattttcg accatccccg gggtggttga ggacgtcacc gacatcatcc tcaatttgaa
    53761 atccatcccc ctgaagctga agggggaagg gccgagcgtc ctgaccctcc gcaaggaagg
    53821 gccggtcacg gccacgtcgg ccgacctgac cggcgacggc gacgtcgagg tcctcgacaa
    53881 gaccgtcgcc atcgcctcgc tcgacaagga cggcctgttg gacgccgagc tcatcgttga
    53941 gaacaaccgg gggttcatca ccgccgacca gaatttcgac gagacgcgga gcatcgacac
    54001 catccccctc gactcgtccc attcgcccgt cctcaaggtc aactaccggg tcgaaccggc
    54061 ccgcgtgggc aagcggatcg attacgagaa gctgaccctg gaaatctgga cgacgggcgc
    54121 ggtcgcgccg gtcgaagctc tggcccaggc ggccaagatc atgcgggatc acctgatcat
    54181 cttcctcaat gtcgtcgacg agcgggccga cagcgagccc gtgacggcca agcgggagat
    54241 cccctacgcc ggccagaccg agctcctggc caagcccatc gagcatctcg agctttccgt
    54301 ccggtccaat aactgtctcc gctcggcggg catcgaaaag atttacgagc tcgtgatgaa
    54361 gagcgaggag gagctgctga agacgaagaa cttcggccgc aaatccttgg gggagatcaa
    54421 ggagaccctg gtcaccctcg ggctcggctt gaacctcgac ctgaatccca agctgctgga
    54481 acggatcaag ggcgagacga aggacaaagc gaaggagacg gaggaatgag gcatcgtttg
    54541 agcggaagaa agctgggccg gaccacggcc catcgggaga tgctcctgcg gaatctggcc
    54601 acctcgctga tcgagaagga acgcgtccgg acgacggtcg ccaaggccaa ggagctccgg
    54661 cccttcgtcg agaagctgat caccatgggc aagaccggca cgctggccgc ccggcgccgg
    54721 gcgctggccg tcctgatgaa ggaagacgcc gtgacccgcc tgtttgagac ggtcgccccg
    54781 cggttttccg agcgggccgg gggctacacc cggatcgtca agctcagccc gcgcaagggc
    54841 gacggggcgg agatggccgt gatcgagctg gtcggctcgg aattcaaggc caaggaaaag
    54901 aagaaaaagg acaaggagaa gggcaagaag ccggccaagg cgtaggcccg ggcaagccat
    54961 tcgcgcccgc cccgccgcaa agcggggcgg gctttttttt tgccgctgga agaacgcggc
    55021 cgcatcatcc aagatccgga atccggtctg cttgcccatg atcacgctcc tggacaccgc
    55081 gggaaaaatc catatgataa tgggtttgaa atcgtcgctc ccgcgaggcc ggattgtcca
    55141 ttcaaagatc gaatcgacaa aacaatcgac gcccgccgct tagctccatg acttgcgcga
    55201 ttcgtaagat ctcgagatca tccccggccg aagaggagcc tgagccgttc tcgtccccga
    55261 cgtcgagctg aggagggagg cgtccatgcg atcgtcgaaa acgggattaa ggatctgaca
    55321 tggccgaccg gatcgatctc ggaatccatc cgcggactcc ctaccgcgtg tccgccgtct
    55381 tagcggccgc ggccgtcatc gcctcgtcgg cgtcgtttta tttctggggt gtcttccgcc
    55441 gggacgcgcc cttcatcatt gggaacatga gggggacggc cctgtccatg ctggtcctcg
    55501 cggggcccgt tctcatcgtc tcgatgatcc tgacggcccg cggctcgctg cgcgcccgct
    55561 ttgtctggct gggatccctc gcctacatcg cctacaatgc cctgctgttc tgcttcatgg
    55621 tgcattttaa ctcgtttttc cttcttttcg ccgccttgct cggcctgtcc ttttgggcgc
    55681 tgctgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    55741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctcgt ttttccttct
    55801 tttcgccgcc ttgctcggcc tgtccttttg ggcgctgctg actctcctgg cgagcttcga
    55861 tctcgccgcg gtccaggcgg ccggttcagg cgtccccggg cggacgatcg cggtctatat
    55921 gatgatttgc ctgatcgtct tcggcttcgt ttggctccag gacatcgttc cggcgatgat
    55981 ccacaacacc atgccggaga gtttcgaggg cgccgggctt gcccagaatg cgatctacgt
    56041 attggacttc gctttcacct tcccgatgct ggcggtcggc gccgcttggc tttggcgccg
    56101 gcgcgcgtgg ggttatccga tctccggaat gatggtgatc atgttgacga tcgagaccgc
    56161 cggcatcgcc gtcgatcagg ctttcgggcg tctccacgac ccctcggcgt cggccggcgc
    56221 ggttccgatc atgctggccc tctccggcgt cgggctgatc ttctcggtcc tcttcctgcg
    56281 agggataaac gaccgaggct ccccgacttc gcgcccggcg aggtgatgga ggccgcggac
    56341 cgctgatacg acaaaggccg tcgatgcttt tgctcctgga gttccaatcc gggtcggcgc
    56401 gactcgccgc gcgcatccgg atcgaggcgc tagataacat ccggcggccg gcccggttga
    56461 tcgtttccct tttcccgcgg gggcctgtcc gatcggtgat gttctccgct ccgcggaacg
    56521 accggaggcg ccctgtcgcc ggcgtaatgc tgacgagaat gaagaatcga aaccatgtcc
    56581 ggttcggaaa caagagaacc cgtgctaaaa tatcatatct aggatatctc gcgacggggt
    56641 gtccgatttg aataatgaaa accttgagaa aggagatgtc cgtgcgtaaa tcgaccgtcg
    56701 gattgttact ggccggtgcc cttgtttttt tcgcggttca agcgtcgggg gaaaccctga
    56761 tgatccgggc aaagaaaatt tatacgatca gcggagccgt cgtggaaaac ggggccgtcc
    56821 tcatccggga cggaaagatc gccgccgtcg gaacggagat cgtcccgcct ccgggaacgc
    56881 ccgtcgtcca ggcctgggcg gtcaccccgg ggctggtgga tattcattcc cacgtcggcg
    56941 tctattccgt gccctcggtg gaagagaaca gtgacggaaa tgaaatgacc aatcccctca
    57001 ccccgcaggt gcgggccctg gattcgttca acttcgagga tccggccatt cccgtggccc
    57061 gggccggagg ggtgacgacg atcgtggctc tcccgggaag cggcaacgtc atcggcggga
    57121 ccggcgtttc ggtgaagctg aaagacgacc ggcccgatcg catggtcctc caggaagaca
    57181 actgcctgaa aatggccatc gaagggaatc ccatcggcgt ctacgggccg cgcaaacaga
    57241 tgcccgccac gctgatggcc gtttaccatt tagcccgcaa ggcgttcgtc gaggcgcggg
    57301 aatacgaggc cgcctgggag aagttcgaga aggagaagaa ggcgggaaag gcggccgcgg
    57361 ctccgaagcg ggagctcggc aaggagacgg tgttgaaagc cctccggcgg gaaatccccg
    57421 tccacatgca ctgcgccacg gccgcggagg ccatgagctg catccgcctg gcccgggaat
    57481 tcaacctgag gctgaccctg gcccattgct attgggtcta tcttgtggtg gacgaactca
    57541 aggaatacaa ggatgtcgcc ttcaacgtcg gcccgccgat gctctttggg tattttgaca
    57601 atccccatgt cttccataac acggcggcta tcctggccga ggccgggctg gacgtctctc
    57661 tccaaaccga cgccttgggc ggcggccagc agaatcttct ccaactggcc gtcctgtgcg
    57721 tccgccgcgg catgaaggaa gcggatgcgc tgaaagcgat caccctgaac ggcgcccggg
    57781 ccgccggact ggacgggcgg atcggcagcc tcgagcccgg caaggacgcc gaccttgtgc
    57841 tctatgacgg cgagccgctg gccttgacca cccgcatcac ccaggtgatc atcgacggcc
    57901 gtgttgagta ccggaacgaa agcctgaaac ccctggccgg attgaccgcg gcccccctgg
    57961 ccgcccgtcc cctggatctg ccggagaacc tcggcgcggc cggggcgttc gccatccggg
    58021 ccggaacggt cttcaccctg gccggggagc cgctgcgcga cgccgtcatc ctggtgaaag
    58081 acgggcggat cgaaaagatc ggccgcgacg tcgccgtccc ggagggctgg ccggtgatcg
    58141 aggccggcga ccgggtggtc atgcccggcc tggtctcgcc gcggtcccac gccggaatct
    58201 ccgacaactg gaaacgtcag agctatgtca atgaaacgtc ccagccggtc gtgcccgagc
    58261 tggaggtgaa aaattccatc gagccgcagg ccccgctttt ctcgttcctc cgcggggacg
    58321 gcatcacttc gctgctcgtg acccccggca acctcaacgt catcggcggg caggggacgg
    58381 cgatcaagag ctgtggagcg gtcgtcgacc ggatgatcat caaggatcgg gcggtcatga
    58441 tgttcggcct cggggcgaac gcccggcggg agggggagat gcccacgacc cgcatgggga
    58501 tcatggccct gttgcgcgag acgctggtca aggcgaggga atacggcgaa aagcgggacc
    58561 gggccgcccg ggataaaaag aatgaaactc cggccgcgga cctggccctg gacgccctgc
    58621 agccggtgct gcgcggagag atgccggtca tgatccacgc cgagcgccgc gacgacatcc
    58681 tggcggctct gcgcctggcc gatgaatttc agttgaagat catcctcgac ggggccaccg
    58741 acgcttacaa agtggtcgac gagatccgga agcgcggcat tcctgtcgtt ctggagggac
    58801 tgttccgagg cttgggccag gtggaggacc ggggattcaa cgaagacaac gccgtcatcc
    58861 tcatccgggc cggcgtgacc gtggccttcc gtccggacga gggttcatgg cggacgcccg
    58921 cggccgggca gcccggcggc gaaccgttga cgctggccgc gttcgcctac cgcggcggcc
    58981 tgaccgagga ggaggcgctc aaggccgtga ccatcgagcc ggcgcgcatc gccggcctcg
    59041 ccaagcgggt cggaagcctg gaggccggga aggatgccga catcgtcatt ctgcgcggcc
    59101 atccgttcct gaccggcgcc atcccggagg cggtattcat cgacggccgg ctggaggccc
    59161 gcgctcagcc ggaccggcgg tgaaccccgg aaggacgcgg ccatgaaaaa gagaaatacc
    59221 gttctttggg cggcggcggt cctggccttt ctgttcgttc gcccggccgg cggaggggag
    59281 gccgatccgc ttctggccgt ccgcggcggc cggatcatca ccgtcagcgg cggcgtgatc
    59341 gaaaacggcg tcctgctcat ccgcggcgga cgcctcgagg ccgtcggccg ggacgtggct
    59401 gttccgcccg gcgccgaggt catcgaggcc gccggggcgg tcatcacccc cggcttgatc
    59461 gacgctttca ctcacctggg gatggaacgg ccggaagccg gcggcggcga ctatgacgag
    59521 tcggccaagg ccgttctccc gcaattgcgc gtcatcgacg ccatcgactg ggaaaatgaa
    59581 gacttcccgg ccgcgtgctc cgaaggcgtc acctccgccc tctcggcccc cggcgagggc
    59641 gcgcttctga gcggccaaag cgccttggtc cgccttcccg gacactcctc cgggggcggc
    59701 gagacgctgc gctttccggc cgcggtccac gccaacctgg gggaagcggc caagctccgt
    59761 tttggaagcc gcaaccagta tccctcgacc cgcatgggcg cgatggcctt gctgcgccag
    59821 actctcctgg acgcccggca ggccatggag agcaagaccg gaccggaccg ggcggcggcc
    59881 gcggccgtcg aatcgctcgg gccggtgctg aaaggggaaa tgcccctggt cgtgcgggcc
    59941 aaccgcttgg acgacatcct gacggccgtc cgcttggcgg acgagttcgg cctccgcctc
    60001 gtcatcaatc acggcgccgc ggcttttcgg gcggcttccg agctggccga acgaaagatc
    60061 cccgtcctgg tcggcccgct ggccgacttt cggagcacgc cggagtcggc ggccgattcg
    60121 ctggaaagcg ccgtccgcct tcagcgggcc ggcgtcaaga tcgcctttca gggaaattcc
    60181 gggcgccggc cggacgcgct gctggagatc gcccgcctgg ccgtcgccca cggccttccc
    60241 gaggaggagg cctggcgggc tctgacgctc aacgcggccg agatcttctc cgtcgacggc
    60301 cggatcggtt cgctggaggc gggcaaggac gccgacgtgg tgattttcag cgccgatccc
    60361 ctccgggaaa cggccgtcgt gcggacggtc atcagccggg gccggatcgt tttttcgcgc
    60421 tgagcgcccg ctggggcgga caaggcggtt tcttgacatc gcttttccgc cggatttatc
    60481 ctgtgtccgc ccggacgacg gccgccgtcc cgcaagggcg gggaggacgg gagcttgccg
    60541 gcgctcaagg aacgggtgag agacgggacg cgcgccgcgg gacgaagggc gttttcgggc
    60601 cggcgcgcgc ccccggcccg ggttgatatc ccgggtttca agcccgcacc ggcatccggg
    60661 aggaggaaga tcatgaaacg aggcttgagg gttgttccgg tggttctctg tttgtgcttt
    60721 tccccggccg tccgggccgc ggaaaccgtc cgcatggagc ggatgaattg gccggacatc
    60781 aagaaggcga tcgaatcggg ctgcacgacc gcggtcgtcg gcgtgggttc gatcgaacag
    60841 cacggacccc atctgccgac catgaccgat gcgcggatca gcgacgccct ggccgagctc
    60901 gtcgcccgca agctgggcgg cgcgcttcaa gcacggacga tcgagaccgg actgtcggat
    60961 catcacctcg ctttcgccgg gacgatttcc ctcaagcccg agacgctgcg gctgatcctt
    61021 cgagattata cggccagtct cgtccgccac ggcttcaagc gcatcctctt cattccgatg
    61081 catggcggaa atttcgaaac cgtggggcgg tttgcagccg aggcccggaa ggctcacccc
    61141 gggatcgacg tcacggcgtt cctggacttg cccggcttga cggacttcct cagccgccta
    61201 tcggccgaat acggcgtctc caaaacggag gccggagacc acgccggcga gaacgaaacc
    61261 tcgatgatga tggctttgga gggcgggctg gtcgcgtccg agcggtttgc tcccgggtat
    61321 tgcgggccgt tcggcggggc cgagatgagg cttctgctgg aaaaaggcat gcccgcgctt
    61381 tccgccgacg gtgttctggg cgacccccga aaggcgtccg cggaaaaagg ccgggtttat
    61441 ctcgagcggt tggcggattt catcgtcgcc tcggtcggcg gaacatccgg acgccggtga
    61501 gaagatttcc gggctttcgc cgaagacgtc catactcaaa tcaagatgat ataaagggtt
    61561 agcttcggct tccgcaatgt cgatatcgat atccgtactg gccgttttga tcccatgact
    61621 gccacaatat tcgtcataga gcccggacgg cctggacgag ttcacggcct tgacagccgg
    61681 cgctcccgat gatatgtttt catctaacat cctccagccg ggcggcgcgt ccgccggccg
    61741 cgggatgttt cgttattagg gagtaattcg ttttctttcg tttaaaaaaa caatcgagga
    61801 gtctccgatg aaggcgtctg ctttgttctc ggaaaaaaaa ccggttctgg gcatcaacag
    61861 cctggggagg atcggcaagc tcaccctgtg gcatcatgtc gggcggaagt atttcaagga
    61921 gatcgtcgtc agccagggcc gggaaatcgg cgccgggatg gacgccgtcg tccagacgat
    61981 cgaaaaagat tcaacctacg gccgcatgca tacgtttctt tacgggatca acgccgagcc
    62041 ccggatccag atcgtcgatg aaaaggaagg caagcttctg atcgacggga tcccggtgac
    62101 ggttctgcgg aaggaccgga atcccaagga catcccatgg cgcgattacg gcgtggatgt
    62161 ggtggccgat tgcaccggac agttcctgga tccgacggcg gaaccggacg cggccaaggg
    62221 atcgctccgc ggccatttgc agggcggcgc ccgggtcgtc ctcgcctcgg cggccttcaa
    62281 gctcaagaac cgggccctgc ccatgcccga ggacgccgtg accctgatct acggcatcaa
    62341 tcaccgggct ttcgatccgg accggcattc cctcgtctcg gccgcctcct gcacgacgac
    62401 cgccctcgcc catatgatca agcccctcgt cgaccacgaa aagactcggg cgatcctgac
    62461 cgcctcggcc tcaacggtcc acgcggccac gaattcgcag agcgtcctgg acaacgtccc
    62521 caaggccggg gcgaaggacc tgagaaaatc gcgctccgtc ctcgacaaca tcatcctgac
    62581 gtcgaccaac gcggccgagg ccctggccct ggccgttccc gaggtgaaag ccatcggctt
    62641 catggccgat tccatccgca tcccgatgaa gacggaatcg atcatcatcc tgaacgtcac
    62701 gtttcaaaat cccggagacg ccggcgtccc ggcgatcgac gcccgggcga tcaacgagat
    62761 ctaccggcag gccgcggccc gcgacccgga acggcgcctg gtgttctccg aggagcagaa
    62821 cgtctcggcc gacgtggccg gcgtcgacgc ctcggtcgtc atcgagggtc agttcaatca
    62881 cacccggacc gccttcatcg acatcgacgt gtcgcagatc tccgccctac cgccggaaac
    62941 ccgggccgct tttcccgtca agcgcctgga gatcccggtc gtccacgcca aagtcttcgg
    63001 ctggtatgac aatgaatacg ggagctacac gaaccggatg ggcgacctgg ccgtccacat
    63061 ccagcagtca ttgaagtaag gccggttccg tccgaaacgc gtttcagacc ttggccggaa
    63121 cggccggctc cggttcgatc cggtggcggc cgcgatagac ggctccgctc cagccgtcca
    63181 ggctgaggaa atcgccgcgg cggatgacgg cgtcgtggat cgtccccgtt ccgctttttt
    63241 catcgacctt caggctccgg agcccgacaa cgccgacttt cttgagctgg gggataatca
    63301 ccgcggcgtg cgaggtgctg ccgcctttgg ccgtcaggag gccttcgact ccccgcagca
    63361 ggccgatgtc gtcgggaacc gtgtccggcc ggatcaagat cagggggacg tttggatcct
    63421 gtcggctgaa ttcctcgatg tcggcctccg agaaaacggc ccggccgcac aaggcccccc
    63481 cgctgatgcc gattccgttg ccgaggcggg atttttcgag cttttcggtc cgggtgaaga
    63541 ccttcacccg ggcgggttcc ggcggcggca gatcgcgcgt ctgaaggatg tagagcccct
    63601 ctttcgtcgc gctttcgaag gtgaattcga tctcctggtg ctcgaatccc ttttcataga
    63661 ccagggtttc ggccaggcgc ttgagctcgc cgtagaccgc cggaaactgg gattccaggg
    63721 agatcggctc gtcccgtcct tcgctgattc gctgcgtttc ggaaatgggg taggtttcaa
    63781 ccaagccggt gacgatatcg tccccttggg cgccgtgggt gaagtcgccg aacagggaaa
    63841 cggagctcgt cagccccttg ggttcccggg tgaagatgac ccccgtcccg gaatcgctgt
    63901 ttaagttgcc gtacaccatg gcctggacga tgacggccgt gccccagtcg ctggagatgt
    63961 tcatctccgc ccggtagatc tcggcccggg gagaatccca ggattcgatc accttgaaaa
    64021 aggccttgtc caattgctcc cggggattat ccgggagggt gattccgctt tggctcagac
    64081 ccttccggta ggcgaaggcg atgtcccgca tctgttccgg cgtgaaatcg aatttcttga
    64141 cgatcccgag atcggccttg aactggtcga tggtgtcgtc gaagaagtcc cggtccatgc
    64201 cctccgacat gccccagaac tggagaaacc ggcggtaaca gtcccaggcg gcccaggcgt
    64261 acctggggcg ggcggccaga gcctcggcga tttgatcgtt gaggcccaca ttgagaaacg
    64321 acgtcatcat cccgggagcg gagatcgccg atccgctccg gaccgaaaga aggagaggat
    64381 tatggggatc ccccagcttc ttgcgggcgg tctgttcgag aattcttaat tgccggtcga
    64441 tgcgctggtc gaggtcccgg ccgatgttcc ggtcgctccg gatggcttct ttgcagcgga
    64501 agacttccgt cgtcaggatg aatcccgggg ggacgggaaa tccgaaggag tgcaattttt
    64561 tcaggaaata tcccttgttt ccgaggagga tttggtcgtc ccggggaccc attttttccc
    64621 cgatcgggac gacggcggcc ccgggatcgt agctcatcag gacgttgcgg agatgcgctt
    64681 ggtccttgaa tttctcctcc tcggcgcaga gcgtccggag gatggtgctg acgaaattgt
    64741 cgatcgtctg caggccgaag gacgaggcga tcagcgagcg gatgaagttt tcggtccgga
    64801 tgtagatctc ctcctcttcc ttgtccggac tggggcgttc ctcttggggg agagcggcca
    64861 ggacctgacg gatgacgagc ggcagtcggg cccggtgggc gtcgatgaaa taaacgtgga
    64921 tgacgtcttg gacgcccttg gagatgaatt ggaagatgtc ggtgtattgg gccatgctga
    64981 accgcggagc cttgagggct ccttcgagga gacggacctt ggacgtcagg ccctcggtcg
    65041 cgatcccctc caaggcgagg accttcagaa agatcacgag gaagcggtgg atttcgacca
    65101 gcgtgctccg ggtgatgaag cggagattca ccggggcgag ggcgtgctcg agctggacgt
    65161 cggccaggct ttggagcttg aggtagagac ggagggcgtc gaatttctcc tcgcggtagg
    65221 tgccgtagac ggacgggatc ccgacggcga tgtgccgctt ttcgtagaga tcctccagcg
    65281 cctcggtctt tttgggggag aggaagcggt ccttcaggat ctcgaggaat tccaggagaa
    65341 ccgccagggc cgggccgggc gtttgccggg ccaaggcccg cttcaaaatg tcgattttcc
    65401 ggcggtcgaa caagaaggag gcgtcgagat ccttgaggac gtcctgatgc tggacgaaat
    65461 acttgcggtg gaggagctga tagagacgga ggaacagggc cgccttctcc ttgtccggct
    65521 cgggaacgtc cgggatttcc cggacggttt tgaggatttt catctggtcc caggcgagcg
    65581 tttcccggag atcgccgccg gcctcccgcg acactccggc catcacccgg tgaaggccgt
    65641 cgtagaatgg cccggagggc tggagcgcgt cgtagatctc cgccgggagg aacggcttca
    65701 ttccttcctt tcggcccgcc gcccaatatt ccaggacggc ttcgcagaag gggacaagcg
    65761 tgctgttgca ctccacgtga gtctgtttcc ggatgaagtg gatgagcgga tcctcccgaa
    65821 agcagagggc gtccaggcgg gtcgtattat cccggagctc gccgccggcg ttgatgtcgt
    65881 tgaaatacac cgggaagagg cgggccaatt gaagaaccag gttgtgggcc ggggtgatgt
    65941 cggcgttgag aagggcggaa atgtccttct ggaggaggtc ggcgtcgcgg acgaagaccc
    66001 cgccgatttt cagatgggcg atcaaggccg acagcagagg cttggtccag cggggcttga
    66061 gggcgatgat ttcgatccag gcccggatgt tcttgacgtg gagagggttg aatttcactt
    66121 cccagtcggt ggtcgccccg cggatgccgg ggccgacgaa accgaaccgg atgatctcgt
    66181 cgagcaggac cttgacctcg ggatggcggt cgagcttgaa gacttcccgg gccgccgtgc
    66241 tgaggcagct catcatggtg tcgggatacg cggcccgggc ggcgctctcc cggaacgtct
    66301 ggaacagcag ggagatcatc ggctcccgct tgtcgggagg aagaccggcg aacacccggc
    66361 ccagggaacg gttgatctcg atcagggctt cgcggtggat gtcccggaat ccggggacgg
    66421 aaatgatccg gaaaaggtac aggagtcgga tttcgtgccg gcggccgtcc gagaaggacg
    66481 ctttctcgat ttcgtcggcg attttcaaat accccttggc gatttggtag taatccggcc
    66541 attcgaggag gccggaaaac ccggcccagt cgccgggctc cgagccgccg tcgcaggcgt
    66601 cgaccaggct tttccattcc cggagccgtt tccggctcag cggctcgacc aggctcaaat
    66661 aaagatcccg gtccgctccg tctccgtcct cggccttgaa gggaatgaat ccttcctccg
    66721 gatcgggttg ggcgagccag taatcgaagg acgaccggaa cgcccggccg gccaagcttt
    66781 gatacgctcc gcgcgaaagg ggaaggttcc tctcgaggca ggttcccagg atcagcttca
    66841 tgtcccgggc cgactttttc aggaggctgt ctccgtccgg aacggcggaa agaacccggt
    66901 tgaaagcccg gtcgacgagg ggaagattgc ggtcgaggtt cggcccgctg tcgttcatga
    66961 tcttgcggag atactcgaac aacccggcga ggcttttctc ccggacgtcc tcttcttgcg
    67021 acggctcgat catctcgaaa aacaggtcga cgacgatttc atgggcttcc cgcccccggg
    67081 gatgggcgtc gaaaacgtga aaatcctcga ggctgatccg ccgccagtcc tcggcccggg
    67141 cggcctcttt ccgcccggtc cggtggtaat cctccaaaaa attgcggatg cggaggtgcg
    67201 ttccgtagga ttcccggacc agctcgatca gcgtccgggc cggagggggg agaacggggt
    67261 cgagcccgcc cgtccggcgg gagtgggcgt ctgtccggct catgagacgt tcctttcccc
    67321 ggatccgctc cgcccggaga ccgcccgata ccgcttgagg agggccgccc cgcggccgtc
    67381 gtccctggcg gcgacgacgg tccggtcggc gaagaatcca aaaatttccg aaaccgagaa
    67441 atccccccgg gccgagtctt tcttcccggt gaagggaaag acatccggcc cgcgctggca
    67501 cttggcgttg aggttgaccc gggcgacctg ggcgcgggcg gcgtcgatga gcggcccgag
    67561 cgtccggacg tcggtcccga agacgctcgc ttgttgaccg aaggaggacg cccggaggta
    67621 gtcgaccggc tcctccatcc ggcggaaatc gacgatcgga acgagcggcc cgaactgctc
    67681 ctcgcgatag atccgcatcc ggggcgtgac cggttgaagg acggcgggac agaggagcga
    67741 gcccgcggac cggccgccgt tttcgttgat gatgtccgcc ccgcggccgg cggcgtcgtc
    67801 gatgagcgac cggagatagg acatccggac agggtcggcc aggggggtga tgcggacgcc
    67861 tttcgcccag ggcatgccga ttctgatttt ggccgtttcc gcagcaaggc ggtcgaggaa
    67921 cggcttccgg accgcctcgt ggacgaagag aatcttcagg gccgcgcagc gttggccgtt
    67981 gaaggccagg gcgcccagca ggcactcgcc gaccgccgtc tccaggtcgg cgtcgggcag
    68041 gatcacggcc gcgttcttgg cgcccaggcc gaggaggacc cgcaggcggg tccgcttcgg
    68101 atgcaggctg gtcagccggt cggccaccgc cttggaaccg atgaaggcga agacgtcgat
    68161 cccgccgctt ttcaagaggg gatcgagaac ggccggcccg tctcccgaaa ggacgtggac
    68221 cgtgcccgga gggaagcagg acgcgagagg ctcgagaagg aaatcgaaaa acaagacgcc
    68281 gaagcgggga ggcttgatga tcaccgtgtt gccggccagg agggcgggca cgacgagtcc
    68341 gaacgtctca aatagaggat agttatacgg cccggcgcac agggcgacgc cgcgcggctc
    68401 gtcgcagacg aggccgatga tgcccttttg ccggatcagg gaccgcgagg cccggtcccg
    68461 tttgaccgcg gcttgaatca atcggccgat caattcgatc gtccggtcga actcggcctc
    68521 gagctcgacc cggggtttgg cgatttccca cattaatgtc cggacgaccg gctccttgag
    68581 gccggccagc cgggcgctga agtcctcgag gcggcggatg cggccggcga gagtcaggga
    68641 gggccaagcc ccgcgtccgc cgcaaaaagc cttccgggcg gcggccaggg cgcggagggc
    68701 gtcgcggccc gtcatccggg gaagggagcc cagccgcagg ggggaaaatc cctcctgggt
    68761 tcggacatcg atgggggagt agacgtcaag acggcgccct ttccagggtt gcagatcgcc
    68821 gcccaggaac acccggtccg gctcgatcgg tttctccatc cggaattcac ggggaatgtc
    68881 ctctttccgg acgaaaagag cggttaaatc ggccggggcc gatccgaggc gacaggctgc
    68941 gccggagaca gggcgagaac aagagaccga cacgcattcc acgggatttc ttctcccgga
    69001 tcatcccccg gcgaggacga tgagatgaaa tagaagacaa tattttacca tatcggaacg
    69061 gtccggacaa tgaaccgcgg cgggcatgtg agagcaaagc aagaatgtcc ccttcgggat
    69121 ggaggcggac ttggaggaac ggatcagctt gagccgtcgc gagatgcggc gtccgtcgat
    69181 cctgcaaaag cccgagaagg gggctgaccc tggggaccgc ggcgggcccg gatgatggcc
    69241 ggaggcgcca aataatgtat gataagccga tggaagacaa aacggcttat tttttcgagg
    69301 tctatggagc cctcccccgg gccggcccgg gcgacgacgc ctcgacggaa aaagccttcc
    69361 ggatgatgaa cggcgttccg gaaaaaccga tgattctgga catcggctgc ggcccgggaa
    69421 aacaaaccct ggtcctggcc cggatttccg gcggacgaat cattgccttg gacaaccacc
    69481 ggccgttctt ggagagggtc gaggccgatg cccgggcgct cggtctttcg gcgcgcatca
    69541 agaccgtcct ccaggatatg aagacgatgg atttcgcccc cgggacgttc gatgtgatct
    69601 ggtccgaggg cgccctctac caaatgggct tcgccgcggg actccgggcc tgccggccct
    69661 tgttgaaagc gggcggctcg atcggcctga ccgagctggt ctggctgacg gacgaccggt
    69721 cgagcgaggc cgtgaaatgg gcctgggact acgcggacat gaaggatgtc aaggacaacc
    69781 tcgccctgtt cgaggcgtgc gggtacgagg tggacggcca tttcaccctg ccggcctcct
    69841 cctggatcga ttcgtattac ggccccatgc gggcccgcat ccgcgaattg cgggagacat
    69901 gccgagacaa ggccgccgcc cgggaggtcc tcgacgcggc tcaggccgaa atcgaaggct
    69961 ttgaaaaatg ctcgaccgag ctcggctatg aatttttcgt ggcccggagg acggaatgaa
    70021 gccggcggac ggtgtcccgc gcccgccgga cggccgggtg aagcaacggc gcatgatccg
    70081 gctcgtcgtc aacctcggct tggccggaaa cgccgtcctg gccgcggcca agctcggctt
    70141 gggaatcctc ggccacagca aggccctcct ggccgacggg atcaattccg tctcagacgt
    70201 cgtctacttc ctggtggtca aattcttcgt cgggatgtcg ggcaagccgg ccgaccgcga
    70261 acatccctac ggccatcatc aatacgaaac gatcgccgct ctcgtggtcg gcgccttcgt
    70321 cgtcacgagc ggcctggcga ttttttggga cgccgtcgat tcccttttcg atttgatcaa
    70381 aggaaaggag acgcccgcgg ccatcgcctc tttcacgttg tggacggccg ccggcacggt
    70441 ggccgccaag ctttttttga tgttccaagc ccgtcgcgcc gggcgggtga ccgacagcct
    70501 cgccgtgaag gccatcgccc gggaccatcg caacgacgtt ctcgcctctc tcggcgtggg
    70561 agccggcatt ctcctttccc tttccgggca tccctgggcc gatccgatcg ccggggcggc
    70621 ggtcgcggtg atcgtggcca aaaccggatt ttccattctc ctggagtcgg ccgacgagct
    70681 gatgaacacc ctcccgagcg aggagatgaa ggagcggttc gcggcctccc tgcgggacgt
    70741 tccgggcgtc gtcagcatcg aggacattcg ggctcatcgc ttcggaccct acctcgtggc
    70801 caatatcatc atccgggtga acagcgccct ccgcgtcggc gaggcggacg aaatcgccac
    70861 ggccgccgag acgcgattgc gagaggacgt ggagatgctt cgcaaggtgt atgtccatat
    70921 ccatccggcg gagggcggcc gcctgtccgg ttgaagaggg gttcgaagac gccgttctcc
    70981 tcttcggggc ggccgccaat acggaggcaa tcgggagaca ccatcccaat gcaccctgtt
    71041 tttgggaatt tccggctcag ggaaatcggg tgaatgggga tggtgtctcc cgattgtcac
    71101 gccccgggca agcgcgggaa aaagacgggc gtcttctttt tatactcctc ccaatccggc
    71161 cgaccggcat actttttctc caggagcggc acgccggaaa cgaaccggag gaagaaggtg
    71221 atgacggccg ggccgatgag cccggcccag ccatatcttg aagacagggc gatcaggccc
    71281 aaaccccacc acagagtcgc ttccccgaaa tggttcgggt gacgggaaag cgaccagaga
    71341 cctcgggtca tgagcatctt tttgtttccc gagtcgcgga tgtgggcggc gagctggcgg
    71401 tcggcgacgg tctcgaaaag aaacccggcc agaaagaccg ccgttccggc ggcgtcgagg
    71461 atggagagcg gcctggccgg ggagaggaag acggccgtca ggggggagga gacgaggagc
    71521 atcaccgccc cttggaagag gaagatgaaa atgtacgact tcagcggggc ggctttgccc
    71581 caccgtttcc tcatggcggc gtagcggaag tcctccggct ttccggcgtt tctccgaaga
    71641 atgtggaagc tcaagcgggc ggcccagagc gtgatcagag cggtcatgag gagacgggcc
    71701 ggagaaaaat ccggatctcg gaggaacaag gccgcggccg ccaggacgaa gccgggtccc
    71761 cagccggcgt cgacgatgga ataattcttc cggaccaggg ccaggacgca gagcccggtc
    71821 atgtagagga acacgatccc cgccgcggtc aaaagcggtc cgatcaccga agccttccct
    71881 tccggcggct cacgcctctt ccatcaggaa cgagacggcc acggccccca cgcccgcgtt
    71941 caggccgatc acgggcgaaa tatccatgag ataggccggc ttccggccga agatctcttc
    72001 catctcaagg gcataaatcc cggcttcctc cgggttgtgg gcgtgaagga cgcaggattc
    72061 ccaaacccgg cttccggccg cggcctttct cgcctgccgg cggatgtttt tgaggttgcc
    72121 ccggcggctg aatgccttgt cggaaacgat cgactcgccg ttttccttca cggacacgac
    72181 cggtttcagg ttcaacaggt tcgccaatcg gccggccatc gggctgaccc ggccgccgcg
    72241 gaccatgtat ttcagggtcc gcacgcccac caggattctc gacctcggcc tgacccgttc
    72301 ggcttcggcc gcggcctctt cgtgggaggc gcccgcctca agggcccggg ctgtccggag
    72361 aaccaccaag ccgagggcgc cggacagcgt ccgggaatca atgacggtga tttttttccc
    72421 cgtcttccgc gaaacccgct cggcaaccga ggcggcgccg gcgtacgtcc cgctcagttt
    72481 cccggacagg tggatggaga ggatcgaatc ataatgggcg gccagccggg cgtacatgtc
    72541 ctcgaacgcc ttggcgttag gttgggccga cgtgggaaat ccgcggtctt cgtccaacat
    72601 cgtgtagaag cggtcggggg agattgtcgt cttgtcgaga taggaacaag ggccgaaatt
    72661 caggcggagc ggaatcacat ggacctgatg atggtcgaga aggaggccgg gaagatcgca
    72721 gctggaatcc gtgaccaggg cgacgtttct cttccggcgg tggaccgttt cgtattgccg
    72781 ctgcatgtca tcggctttct gatggacgca gagcccgaaa tcgcggatcg cgtaaaagat
    72841 ttccggaggc tcgtccgcgt ggacgtggag gcggagggtg tccctggatc cggcgatcac
    72901 cagggagtcc ccggcttttg aaaccaggga actcaaggcg tcccggtcca atcccgcggc
    72961 ctcccggccc ggatgggggc ggatcaaggc ctcgacacag tagcgccggc cgacgggggc
    73021 ggccgagacc gcttcggtgt gttcgatttc gatcgcctcg gcccggatcg agacgatctt
    73081 cttatgcgag ggcttgatca agaaatcgat cgccccttca agaaagagga ccacgccctt
    73141 ggagccggcg tcgacgacgc cggctttctt catggccgcc aaccggtgtc gtgtttcctg
    73201 gagggcttcg agcgcccggc cgcgggaatc ggccaagacc ttcaaaaaat tttcagcttc
    73261 tttctcccgg gattcgagat actcggccca ccggcggatg accgtaatca tcgttccctc
    73321 gaccggctcg gcgatcgctt cgtacatata gcggaccgct ttcaggacgg agcgggcgaa
    73381 gcgggccggc gaaacctcgg tccagtctcg ggattcggcc ccgagaccgt aaagaaattg
    73441 ggcgaaaatg acgcctgagt tgccccgggc gccctccagg gcggccgccg cgatcgccgc
    73501 ggaggtcgcc ccgtaggagc gatgcggacg gactcgctcg acgacggccc ggagggtcga
    73561 ggcgagattg gagccggtgt cggcatcggg gacgggaaaa acattgatcc ggttcagctc
    73621 attctgattt tccaggatct ttttcgtccc ggcgagaaag gcgtaataat agctttttcc
    73681 gtttgaccgc tgggatgtcg tggacatcgc cgatcgtccg aatcagccga tcttcaacag
    73741 ccgggccgtc ccgacgatga gaaatccggc gaacgccccg aggaccgggc cccaaatgac
    73801 ttcgagaacc gtcagggaaa ggctccagtc ccggacgatc cagtggttca ccaatccata
    73861 cgcggaaatc ccgagaacgc cgccggccgc gcaggccagg attcctttga gatagggatc
    73921 ggccgaggaa agctgaaggg aagcgaggac gacgaaagcc gtgatcacca ggacttggga
    73981 gagcgctccg ctgatcccgt tcaggacgag cttgccgtcg acgaccgcgg cgaccttgcg
    74041 gatgtcccgg cggtagatcc gtccccagag aacgagatgc cagaaggcgt ccacggcgct
    74101 tacgccgatg aaagaaacaa tccatagtac ggcgtatttg agaatcatag aacctttcct
    74161 tgccgggaag atgatacgtt catccccggc cggtgtcaat gcggtttatc gacggcctgg
    74221 cccggttttc ggacgggtcg gttttgctct tgacgatcgc cgagctgatc cgctcttgac
    74281 actcgaaagc catgatccat cgcggccgtt ctatgtggag gcgtctctct tataatgagg
    74341 agacacaatg cctcttcacc taatttcagt tgatatccgg ctctgggaaa cagggaaaca
    74401 gggtgaaaag gctttgtgtc tccctctttc gatttcagcc gcggctgggg atgcggagga
    74461 cttgtccggc gcggatcaag gttccccgga tcccgttgat tttctgcaag ttctggacgg
    74521 aagtcccgta ccggcgggcg atggttgaaa gggtttcccc cgacctcacc ttgtgcgtcc
    74581 cgtattcggg cggaatatat ttcggcagag cgggcagcgc ggccaggaga cgctcctcca
    74641 tcccgacggg gacgcggaga tcgtacggcg tgtcgggcgt ggattgataa cggagctcgg
    74701 ggttgagaaa ctcgagctcg gaggaatcca agcccatggc ggccgccagg acgctcagct
    74761 tggccgggtg ctggatccgg accgtgtcga agcggagcgg cggatacggc gcgggcaggc
    74821 tcaccccgta tttttccggg tcccggatga tcaagacgac ggcgatgaag cggggaacat
    74881 accgggccgt ctggtaggga aggcgctgga aaagatccca gaaattatcc aggtagctga
    74941 tgttctgcga attgatgatc cgctggacat atccctcgcc gcagttgtag gcggccaggg
    75001 cggtcgtcca atcgccgaaa aacgagtgga gatgttccaa gtacttgatc gcggcgaagg
    75061 tcgacttata ggggtcccgg cgctcgtcga tgaacttgtc ctggttcaag ccgtaattca
    75121 ggccggtcgt ccggatgaat tgccagatgc cgagggcccg ggccgaagac agagccaggg
    75181 gtttgtacat gctttcaacg atcgggagcc agaccagatc cgaaggcagg ccggctttct
    75241 gcagctcgtc gacgatccaa tccttgtaaa atccggagcg ccggtagcct tccaggaagg
    75301 ccgtccgctc ggggccggtg aaggaccgaa tctcccggag aacccattcg ttctcgatca
    75361 aggggatcga tttgtgattc tccacggccg gattgcggcg ggcggcgctg atttcctgga
    75421 tgcgctgggc gatcagcagg cggaggttgt ccttgtcctg gatcaggggc gaatcggcgg
    75481 ggatgcggag gcggagaagc gcggcgtaag cctcgtccag gtaagccacc gctccttcga
    75541 aatcgcctcg ttcccgggcg gccttggctt cggcgtacag ctcgaggacg tcctcgatcg
    75601 tgatcgaggc gtcatcggcc gcctggccgt tgaccggaag gacgccggaa gccaagtttt
    75661 tttcctcgac gggaatgatc gttttcccgg tcgcctcttg gaccggagcg ggcgccgctt
    75721 tttcggacgg cggcgcttca gggacggcgg ccgccttgcc cggcggcggc ggaaccgttc
    75781 ggacggccgt ctcattgcgg gcgcagtcga cgagaaagag acagccggcg aggatgagga
    75841 tgatcggcag gaatttcaat gatttcatga gatcaatgaa cttttataac acgcgggcgg
    75901 aggggagtca aacaaagagg gagccggagg atcgcgttct ccgtcagcga ccggcttcgc
    75961 cgccgcccgg acgtttccgc tctccggatt ccttgaggtg ccaagcccag gcggtctcga
    76021 gaatcgtgcc gaggtcggaa aagcggagcc tccatcccag gacgcgctcg gctttttcct
    76081 tcgaggccag aagaatggga gggtcgcctt cccggcgggg ccgatcttcc cggggaacgc
    76141 gccgcccggt ggctttttcg gcggcggcga tgacttcgcg gacggaatat ccccggtttg
    76201 tccccaggtt gatgacctcg ctcgatccgc cgtccaggag acggcggagg gcgaggacat
    76261 gggccgaggc caggtcggtg acgtgaatgt aatcccggac ggccgtcccg tcggccgtgg
    76321 ggaaatccgt cccgaagacg ccgaggatgg aaccgtcccc ggcggcggcc cggaggacat
    76381 tggggataag gtgcgtttcc ggatgatggc gctcgccgag ctccccgtcc gggtccgccc
    76441 cggccgcgtt gaagtagcgg agggagacgt gcttgaggcc gtaggcccgg tcgtaatccc
    76501 gaaggacctg ctcgatcatc gccttggtcc ggccgtaggg attgaccggg tcgagggggt
    76561 ggtcctcgcg gatgggcgtc tcccggggca ggccgtagac ggccgcgctc gagctgaaga
    76621 tgaacacccg gacgccggcg tccagggccg cccccagcag gttcagggaa gtccggaggt
    76681 tgtgctcgta atatttgcgg ggatcgacaa aggactcgcc gacctggatg agcgaggcga
    76741 aatggagaac ggccgagaaa ccgccctcct tgagcaggcg cccgatcagg tccggatcgc
    76801 gcaggtcgcc ctcgatgagc tcggccccgc cggccgcgtc gcggtgcccg cttgaaagat
    76861 tgtcgaagac cacgggtttg aagccggccc ggcggagctc cttgaccgtg tgggagccga
    76921 tgtagccggc tccgccggcg accaggacct ttttcatggg cgctcctttg ccggccgttg
    76981 ttcccggacg aggcggcggg ccgctttcag ggcgccggcc cgggtgtcga attcattatc
    77041 cagctgccgt cggtagagat ccttgagaag cttgcccatg cccggaccgg gggggacgtt
    77101 cagcttcaga agatcccgcc cttgcaggag cggacggatc gtttccctgg agacgttgtg
    77161 cttccgcatc gactcgagca gccaccgggc ctcgcggtcg agcccggccg ccggccgctc
    77221 ggcccgtccg gcccggtcgg ccgcgtccag gacggccgcc agttcgaact cgccccccgt
    77281 gtcggcggcg aagcggttga acgccttctt ggtcacgatc gtccggttgt tccaaagctc
    77341 ggccgcccgg tgatgcgtcc ggatgagacg cagggcgagg tcgcggacgt cggccccgtt
    77401 ccaggcgtgg attttcaggc ggtcaaagac ccggagggcc agcttctcgc cggccgtgtc
    77461 gtgtccggca ctggtgacgg ccattcgtcc ccgcttgtat tcccaatgcg tcgtctccgc
    77521 cttgccggcg tcatgaaaca gggcggccca gagaagagcc ggccgcttgg ccggatcgag
    77581 atcgagcttt tcggccagcc gcttggcctg gtcgacggtg agctttgtgt gggcccagac
    77641 cgtgtggtgc ccgaaatcgt ccttttcggg atgaaaaacg gaatcctgga tgaccaaggt
    77701 cagggcatag agctcgggga gaagggcccg cagggctccc agccggaaaa gctcctccaa
    77761 gccccgcgaa ggccgggggg agcggagaag gattttaaaa agctcgtcgg cgatccgttc
    77821 gacgctgagg ccggagaggt cgactttccg ggcgatgggg tagacctcgg gatcgatggc
    77881 gaagccgagg acggaggcga accgggccgc ccgcagcacc cggagcgggt cctcgggaaa
    77941 agccgccggg tcggtcaggc ggaggatttt ctcccggatg tcccggcggc cgttgaaggg
    78001 atcgatcagg gctccgtcgg acagacgcca agcgaggctg ttgcaccgga agtcgcggcg
    78061 ccggagatcc tcttcgacgg ggagcatggg atcggattga gcccggatgt ccttgtgccc
    78121 ccggacgctc ttgaccgcgg cccggtcgat tcgggggagg gcgatatcga cggtccgtcc
    78181 ggcccgggtg aatttgatca ccccgaagct tttccccacc aggtcgacct tgcccttcgg
    78241 ctccagaatc ccgatgattt catcgagggt ccgccgggtg acgaggagat cgatctctcc
    78301 ggagggcttc cggcggagaa tgtcccggac gaacccgccg acggcgtaga cgtcgtcgcc
    78361 gaaaagccgg acgaagaagc gccggtcttg gaagtcaggc tcaagcttgg gcatcggtcg
    78421 gaaatttccc cgcctattat accggaaatc gcctttccgt cgatttatgg taaaatattt
    78481 ctttcatacc ggcggagaga acatgaagaa agctcaagtc gcggcgtggc tcggtcttct
    78541 ggcggcgctt tcggccttcg gccaaagggc ggttccgggg gtccggcttc acgaaaaacg
    78601 gatcgtcgat ccttcccccc gggggctcac tcttgttttc cgcctgattc tgcgcaatcc
    78661 ggcggagacg gccgtccggc tgacccgcta cgattaccag gtggtcatcg gagacgccga
    78721 atacctcaag ctccaagtcc cgctgcccga gcccatccgg gtcgaggccg gaagcgagat
    78781 cacgatcgcc cttccggtca agctgaattt cgcccatctc ttccccgtcg tcccggactt
    78841 gaaggacaag gaccaagccg tctgttatgt cgccggaggg ttgatcttcg cggatgatag
    78901 gggaagggaa aggcgcgtcc ccgtggcctt ttcgggggat ttcccggttt accgcggcct
    78961 ggatttcgcc cctgttcccg tcgaggcgaa atc
//
LOCUS       DAOOXH010000002        71770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_321_length_71770_cov_7.725148, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000002 DAOOXH010000000
VERSION     DAOOXH010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 71770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 71770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71770
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_321_length_71770_cov_7.725148"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..587)
                     /locus_tag="PKV64_00455"
     CDS             complement(6..587)
                     /locus_tag="PKV64_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HPN15580.1"
                     /translation="MNIAICGLDCSVCPAYVVHQTGDAALREKTAEEWKKQYGAEITP
                     EMVDCAGCTVLEGPHIGYCFECEIRKCGLEKNVANCALCDLYPCGVVSAFIDKVPPAK
                     ANLEKIRAEAGAVPSAKTGVAAKPRGKIKPKAKPKGKAKAKPKVKPKPRVKPKAAAKA
                     KAMAKPKAAKKPMKKVKPKAKVKAKAKSKTKKR"
     gene            complement(656..2005)
                     /locus_tag="PKV64_00460"
     CDS             complement(656..2005)
                     /locus_tag="PKV64_00460"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPN15581.1"
                     /translation="MKSFPRRASSGRRPARPWFRFPAAALLAALGGFAAGPAFAGAQA
                     PILKKVLPNGLTVITQTDASSAITVVEIVIGGGASAEPGGRAGIAHLTARLSVNIPDE
                     TTSRDFTVKALKSGMGARDDDAVIHLEFLTEFAEPMLATISKIFNRPIFSDIRMNRLV
                     ESMEHQRRIQIDDARYESHLVQRETFFGDSAYAGETLGTEESLRALRVRDARDFYERL
                     FVAGNITIVAVSDLAPDALGALIERHFASLRPGAPPPPPAALVLKDPPYPPRIVIKKQ
                     QQSVVSCGFILPSMSRRDYVRISLIENALGRGVGSRLWTLRVDQKLAYNVFAQAFFFR
                     RAGLFEAYLETDAPKTASAREALAEVLRDFQERGLTAEELEAGRAILWSGFLRANETK
                     AARATTLGWYESMGLGADFFAHFRDELYSLTLDEVNAEIRRLMDPARASWVTVGPEQ"
     gene            complement(2002..3393)
                     /locus_tag="PKV64_00465"
     CDS             complement(2002..3393)
                     /locus_tag="PKV64_00465"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPN15582.1"
                     /translation="MIERKAERRPAAIQGAALLLLAGAATVLLFPPLVRPASSPQKQF
                     TLDNGLRVFLLERNSVPLVNIVAGVNAGSKDETDETSGLIHLLEHYILFRGTDVRSGS
                     EIARDIRSRGAVFNAHTGQDAAFFDLTLPAEHFLFGLKNQKEILFRMKIEEDGLNAEK
                     EVIGEEIRQIEDDPFRLGQALVFQNLLAGHPYGRPIYGDPGCLADLTAGDIERFYKRL
                     FVPNNTVLAIVGNLPVDEMEARVREVFADVPRGEDPPPPPEFARPPSKTVEIERELDI
                     SEAYLFVGVLGPDYNDPGQYAADVLTQILGQGIHPMMTVALKNSRRAGVNSAHLSYIA
                     LKRAGAFVAYITLEPSKISVAKRETITFLKGVRRENFSPQDVLIEDRAAAFDLLESAK
                     NKIRFLGQQAWESGLGLASSMAAHMLLAGTESEIDYLERIAAVTSGDLRKAAGASFNR
                     DAFVIVSIVPRKK"
     gene            3915..5279
                     /locus_tag="PKV64_00470"
                     /pseudo
     CDS             3915..5279
                     /locus_tag="PKV64_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005983317.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
     gene            5285..5647
                     /gene="rnpB"
                     /locus_tag="PKV64_00475"
     ncRNA           5285..5647
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKV64_00475"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5793..6857)
                     /locus_tag="PKV64_00480"
     CDS             complement(5793..6857)
                     /locus_tag="PKV64_00480"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HPN15583.1"
                     /translation="MKKTLINLLSPLMIAGPFVFGQDIRLIDKIPVSQQDCLIEADDG
                     IAVLPNGSFIICDTRAGNFKIFDKNGRFVKIFGRRGNGPDEFSYPTIFDYSDNKLLIS
                     NSEKYRILVYHVNEDLEIKLGEEFFGYCCDARIFPDHYLFSGSKADPAGKWYEIYLSY
                     PRQKKHSFLLTIQDLFNISPNAGQPQLAEKASMGVRSYCDSHGDQVYAVWEGLLSVIR
                     VDTKTNKQYRFGNKTSLYTAPVASKELIQAKKERNLKKYYKVLHKYSMIRKIFVTDRY
                     IGIVYMNYDREIDLLNPIVQLYSLDGNFIMEKKLDEVTYPHRLMSLYYGKKTNVLSAL
                     SLKIDDQAESHYTLLKYQII"
     gene            complement(6964..8055)
                     /locus_tag="PKV64_00485"
     CDS             complement(6964..8055)
                     /locus_tag="PKV64_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15584.1"
                     /translation="MLALQNVLFLSSRLYRLYGQRGESMVVAAVHNRVLRRIFSTFEL
                     SIEARRREMKKRFLFVSFLPMVLCFAHSQNLILEREIDLGFFLKPHQAHLVFAASDSA
                     GNVFITQRGNDSFLKLDRAGKVVLRAPRRNEGEIIKFDIDGYGNSVCVFSGRLEEGKF
                     RLPLAWYNGSTGEKLKEINLGERFSFIAHLRILRPRDLILVNGIASEEGFRNDSLHLL
                     DFDGNHVNSFSPFGNQGLTVEAILERNGDYFASSPQFDSTNGIVFQALPEMKLIRYFD
                     LAGNRLGETDWAERDHFLLHSGNIWFQGAEGFRVVEKKGNQYVPTTVNIKDADGARVP
                     WHPVAVDSYGNIYFLGGKDLQSLIIYALK"
     gene            complement(8225..9001)
                     /locus_tag="PKV64_00490"
     CDS             complement(8225..9001)
                     /locus_tag="PKV64_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPN15585.1"
                     /translation="MRRNSARNRGKVLVLISKRDKPDFINRLIPFPASLRVVESRNRF
                     PSQSGFSLVFHVCDYSCLTRNCLRSFISLQRLNTPIVVLRPAIFSKAAGFSKSFILTF
                     FDDSVLSPRQREMKETCERSRYYGWGQGRLHPSDPLHKVLLIQKDVVENPWRNHDLAG
                     ISKLIHRSPSWTSSTFKELTGVSLCRFVINNRFCYSLWKILSSDKSIKEIALDLGYRP
                     LSFSGRFRSQFGIRPSRVINKYAYLSRGGSSSIKNMQIKK"
     gene            9094..9140
                     /locus_tag="PKV64_00495"
                     /pseudo
     CDS             9094..9140
                     /locus_tag="PKV64_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9206..9481
                     /locus_tag="PKV64_00500"
                     /pseudo
     CDS             9206..9481
                     /locus_tag="PKV64_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(9475..10011)
                     /locus_tag="PKV64_00505"
     CDS             complement(9475..10011)
                     /locus_tag="PKV64_00505"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HPN15586.1"
                     /translation="MNDRAPLLSRAVRGKKGFRTAVFVLVLAAAAAGPAAAQQAVILV
                     RHAEKADSSKDSALSEAGRARAEALAVLVAPLGITAVYASEYRRARLTAEPAAAALGL
                     KVQTYAAGDAAGLAERLRTRHAGDTVLVIGHSNTLPELMRRLGHPSEEIIADDDYGSL
                     FVLLPQTGDTPVVVRLHY"
     gene            10289..11389
                     /locus_tag="PKV64_00510"
     CDS             10289..11389
                     /locus_tag="PKV64_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPN15587.1"
                     /translation="MNDIEKALMHALFVHLHHNIDKAIDFKAIDALNKKVGKLFPGSL
                     VVGPGDDAAAFKFPGTPGMFVGKMESHCSPCVPRPYDAAATGAGGSMRDVVAMGARPF
                     FLMNFIGTKPLDEKVIVGPCGFKGKCTCGQCREMTSGERVNLMVKGLKDMCRAMDVFI
                     VGGGFSTSFSDIVPAVVVAVMGRLVTPRPLTKPAKAAGSQIILIGRTGSDGNDTLYRA
                     GLVREMKPAVAQFREERKVMEASLAAFRTGKIRACSDLGAAGVGAGVCESARYGGFGA
                     RVDLSKVPLKEKNLSPEEILICETQARMVVQVAKKDVDLILKTVRAKGVRAEHIGEIN
                     NDDKEVFEYRGKTVAVIPNRPSAADMKKLRAV"
     gene            complement(11496..12662)
                     /gene="galK"
                     /locus_tag="PKV64_00515"
     CDS             complement(11496..12662)
                     /gene="galK"
                     /locus_tag="PKV64_00515"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HPN15588.1"
                     /translation="MDDLIQRLRKEHQAVFGVEPAVVAYAPGRVEILGNHTDYNEGFV
                     LSAAIDAGIAFGLSPADEDACTLRAADFGESVRFQAVNPGRTDRERWSNYPRGVLAFL
                     RERYGFRPRGFQGTFCGDIPVAAGLSSSAALEISSGLAFSAFHGIVAEKLALAKIGQR
                     AEHEFAGVRCGLLDQISSLFGREQALVFTDFRSLEADTVSLPQGTAFLIADTGVKHTL
                     VDSEYNERRRRCEEAAAAFRRILGPGIAALRDVTRADMMKYRDRLDPDVWKRAAHPVG
                     ENERVLTGAEALKRGDAAAFGRLMFESHESSRKYFENSCPELDALVEAAGKIQGVLGA
                     RLSGGGFGGSAVVLVEKDRSPAIAASLASAYAAARGRRLEPRVVLPSAGARLVT"
     gene            complement(12681..14108)
                     /locus_tag="PKV64_00520"
     CDS             complement(12681..14108)
                     /locus_tag="PKV64_00520"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HPN15589.1"
                     /translation="MSGPVVLAPLLFLAGGAVLVLLLGTRRGGGSNSVQAAVSVAAAA
                     AAGFAAARLWGRPAEAFGGQLRWDEPALFLAFLYLLAAVLAILLGMRFIVLRRIPRAE
                     YYGLLLLAVCGLILMTATRHLLVVFLGLEVFSISAYALTGLNRRDDGSAEAAAKYFLT
                     GSFASAFFVFGLALLFGASGTLDLDAAAAGLAGTGAVPALAAAGLILVLCGLGYKIAV
                     VPFHMYFPDVTEGAPTPAAAFFAAAPKAAGFAVLLRILFAFARAGIGAGPIASFLSAA
                     AVATMILGNFAALRQRNLKRLLAYSGIAHAGYMLIAVVSGDPAGLLFYLANYLFMGVG
                     AFAAVIALAGPKRETLELDDYAGLGRRSPWAAGIFTVILISLAGFPPTGGFLAKFFVF
                     SAAVRAGRLGLALVGIAASLVSVAVYLRIVRVMFMKEPEREIEVDTENYPVYLALFFC
                     LYAVLQLGLFPGGVMTFLRGAVAGF"
     gene            complement(14105..15670)
                     /locus_tag="PKV64_00525"
     CDS             complement(14105..15670)
                     /locus_tag="PKV64_00525"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HPN15590.1"
                     /translation="MNIPLLSLITFLPLAGAILLIFIPAAERRRLFAAALAVTLAVFV
                     LACLLAFRFDGREAGPQFVEKSPWLGYGLGYHLGVDGLSLFLVLLAAFLTPLVVWASV
                     SSIRDRLKEYLLFLLLLETGMIGVFLSLNLFLFYVFWEAALIPMYFLIGVFGGKRRLY
                     ATTKFVLFTMAGSLLMLAGIFVLHGAYARATGTYSLDMFDLGRMVLSPSAQTWLFLAF
                     GLAFAVKVPLFPFHTWLPDAHVEAPTGASVLLAAVLLKMGAYGFLRLGFPLFPEAVAR
                     LAPVLSGLAVAGIIYGGFMALVQKDIKALVAYSSVSHMGLILLAVTAWNTVSLQGAIL
                     QMINHGLSTGLLFFGVGVLYERTRARGVSDFGGVARKMPVFAALFLIAALSSLGLPGL
                     NGFAGEILCLSGVFAADKILAALAVTTVILSAAYLLGMYRKVFHGPVASGRVAALRDI
                     GGRELAVFLPVILLMFWIGLFPGTFLKKIEATAVRYADALQNRRTAYVRVDGRPAFPA
                     IVSGSGGGEEKAR"
     gene            complement(15672..17552)
                     /gene="nuoL"
                     /locus_tag="PKV64_00530"
     CDS             complement(15672..17552)
                     /gene="nuoL"
                     /locus_tag="PKV64_00530"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HPN15591.1"
                     /translation="MPAFVFIPLAPAVGALVLFPLGRRLSRRSVSTLACGSVAASFVL
                     SVAAALSGSGVRIHRLFTWIQAGGFRAEAAFRFDPLAAVMVLVVTGVGLLIHVYSAGY
                     MSRERDFGRYFACLNLFMSAMLLLVLADNLVLMFVGWEGVGLCSYLLIGYWFEKPAAA
                     DAGKKAFLVNRIGDAGFLLGILVLWSAAGTVSFAGLSEAVASGALAPSAAAAAAVLLF
                     IGATGKSAQIPLYVWLPDAMEGPTPVSALIHAATMVTAGVYMVARLNPLYAASSSASG
                     IVMLVGALTAVYAGVLALVQNDIKRVLAYSTISQLGYMFLGCGAGAYAAGIFHLFTHA
                     FFKSLLFLAAGSVIHALSGEQDLRKMGGLRKLLPRTYPAFLVGALAISGIPFLSGFFS
                     KDAILLTAFAGGHVFAGILGLAGGGLTAFYMFRLVFLAFFGSRRFSAEVESRLHESPA
                     VMTRPLLILAFCSIVAGYVGLPALFGERANLFFRFLAPVAGTPASGHAGPGAEAALLA
                     ASTISALAGLFLAYLFYVRNPALPGRVSARFPGFHHLLLRGFGVDALYETALLNPLLR
                     GSKAVYRRFDLGVIDAAVNGAGGAAGLLGKALALLQTGWVKDYALAILLGAVAFLGVL
                     LF"
     gene            complement(17556..17855)
                     /gene="nuoK"
                     /locus_tag="PKV64_00535"
     CDS             complement(17556..17855)
                     /gene="nuoK"
                     /locus_tag="PKV64_00535"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HPN15592.1"
                     /translation="MSPAAVVGLSLVLFVLGVIAFFVKRDLLTQFMAVEIMLNAANLA
                     FLGLARGSARIDAQVIVFFVMTVAAAEAAVGLAIILHLYRRRKSIRTDELDKLRG"
     gene            complement(17852..18358)
                     /locus_tag="PKV64_00540"
     CDS             complement(17852..18358)
                     /locus_tag="PKV64_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HPN15593.1"
                     /translation="MAADIVFWILAVVSAGAAAAMIISRNPGHNALFLVLCFSALGGI
                     FGLLDAPFAAAVQILVYAGAIMVLFIFVVMMVDPRTPAPPERRKRAKILGPAIGLVLL
                     FELGTAAWGILANSNRSPASGPGVAPADVGRRLFTEFAYPFEITSVLILAALVGAVVL
                     GKKEEPES"
     gene            18867..19772
                     /locus_tag="PKV64_00545"
     CDS             18867..19772
                     /locus_tag="PKV64_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPN15594.1"
                     /translation="MRLMKRKGRIAAVGFAFFTLLLSAAVLQAATVFNIVLDRGGVLL
                     SGRLRVCEGDGPFPTVILLHGLPGNESDVLGLGAALVKNGINVLTFNYSGTFESQGEW
                     SFADTLKDIEAAFYFVNRPDNILRFKIDARRLGLGGYDYGGGMVLAYAAGRPEVGVVF
                     SIAGTDHGEFLREYARNPEMRKIVDETYAAMAAPAGPVRFAEGAVPREWADKGPAALD
                     PNLDFLRIAPALAGKDILLVCAWDDETNKVERNALPLYRALKERKAKSASIAAFQDDH
                     AFRKSRAGLARAVGDWLKSVFFPTV"
     gene            19859..19935
                     /locus_tag="PKV64_00550"
     tRNA            19859..19935
                     /locus_tag="PKV64_00550"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19893..19895,aa:Met,seq:cat)
     gene            20413..22068
                     /locus_tag="PKV64_00555"
     CDS             20413..22068
                     /locus_tag="PKV64_00555"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPN15595.1"
                     /translation="MKTIRLPAAAAALWFFGTAVLAAQDAGLVAITVVKGDTLNNICR
                     AYLVERAVWPKIAVLNRLANPNLIFPGQKIDIPLEYIGGFEDEGTVTIAVGEAVRRAK
                     KDKEWTPLRPGDVVHSGDELKTGGDGGLFISFAGGNTLQLMPEARLTVLVTKKIDDLN
                     IFQRILLGAGKAVARIKDVTGRSSRFEVKTPSAVAAARGTVFRVGLDETETTRAETLQ
                     GQIGVEAQAVLEIVGEGEGTIVHKDQAPQKPRKLPPPPAVLDIPAEEQFPPLRFRFSK
                     VEEAAGYRVFLARDAEGREIILQAAVRPEDAAVFDGLLHEGTYYLIAQSIDADGLEGP
                     GSAPVPVRVGIPLPPEPSSPVEGAMFTATFIDVVWPEPPRTGRYHLQIAEDEDFNRLV
                     VDRTDLAAAVFRTPALSPGFYRLRASAVYGQGRESAFTAPRRFQIIALPDAPLLSEPD
                     IDGRRVFFTCTNRGPGIVYRFQLARDDRFLGLVEERLSPDPEIAFLKPDEAGLYRARV
                     AAVAPSGVESPFSAVRTFRVLKGGVFRFLCLIPAIALLLVLLI"
     gene            22077..24062
                     /locus_tag="PKV64_00560"
     CDS             22077..24062
                     /locus_tag="PKV64_00560"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HPN15596.1"
                     /translation="MATHRRSWLPPFGSLPFLSRRRLIPVIAGLLLFFGADYLGVFDS
                     WDIRAHDAFFRVRSSRPTSEAIVLVGIDARSLGVLGRWPLDRDVYARLLDKLDEAAVV
                     GFDLILAEPSAGDERLAEAVRRNGRVVLACYFDEALNLVLPAGPLAGTQVGHVHIERG
                     IDQISRSLFHTISRPGGPVPSFSAAVYGMFRGGRPWAGRDPLPDFRPDGAAGRLFQTD
                     HSRINYYGPPGTFLRISLADVLSGGVPESFFKGKIVLVGATAAGLQDRSATPFSGTRQ
                     EMPGVEIQANALNNLLDGTEIRDFPLPAAWLGAFLTALLGLALFIRTPERTAAWIWAA
                     GLAGITAASYLLFARFHLWLGPALFYFMTAYVFLAAYLLKLAAAARQLDVKYASLAGH
                     FAGSGDLGRRGGKKGLIGLFTRNGINEKISGLLEIEKSYEGTLEAAVWRRTRELAEAL
                     SMVGRMSNELIVRLARAVESRDAGTGNHVARVALYSRIIAEALGRPLDYVEAIAFTSS
                     IHDIGKIGIPDDILLKRGLLTDDEKEIMRTHARIGYGILAASEHPRIREAAAVALAHH
                     ERWDGSGYPAGLKGEEIPPEARIVALCDYYDALRSERSYKSGLDHDTAVRTILEGGPD
                     CSPAQFDPEVLQAFRKSADKFDDVFRRHRDDAPPAVF"
     gene            24136..25524
                     /locus_tag="PKV64_00565"
     CDS             24136..25524
                     /locus_tag="PKV64_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HPN15597.1"
                     /translation="MFDAKTYTQRRRLLRKRLGSGLALFLGNEESPMNFADNAYPFRQ
                     DSTFLYYFGLDTPGLAAVLDVDGQKEILFGDDIDINAVIWMGDLPKMKDRAASVGVRL
                     TRPAAALAGVLQEAARKKRKIHFLPPYRAEHLQKLESLLGLKPARVSAAVSIDFIRAV
                     VAQREVKSEAEVAEIEAALFTTRRMYDAAMRMAQPGVREMDIAGRMEGIAAAGGRGLA
                     FPIILSRDGQILHNHSHIHRLKKGDLLVVDAGASSPCYYASDITRTIPVGGNFTSRQL
                     DVYEIVRAANENAVAAVRPGRSFKDVHETAALTIASGLKELGLMKGDVEAAVREGAHA
                     LFFPHGVGHQLGLDAHDMENLGEDFVGYDEATKRSGQFGLKSLRLAKPVRPGFVVTVE
                     PGVYFISALIDLWQAEKKHADFIAYGKLESWRRFGGIRVEDDVLVTAASRRILGKAIP
                     KTPVDLAAAIRL"
     gene            25562..26044
                     /locus_tag="PKV64_00570"
     CDS             25562..26044
                     /locus_tag="PKV64_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HPN15598.1"
                     /translation="MVSWFVLCTKPQKEFQVERLFQEAGFPFYCPRFKSSGRIKPFFP
                     CYVFLRFEYPEQFKLVKYTRGIRRVVGNDAGAVPLEDRFIEELQSREISGLIELEKYG
                     VEPSPGDEIEVADGPWKGLRGVFKKSLSDRDRVMILLNYVSYQGQLLIEKSKLKKVLS
                     "
     gene            26041..27213
                     /locus_tag="PKV64_00575"
     CDS             26041..27213
                     /locus_tag="PKV64_00575"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPN15599.1"
                     /translation="MNVSLLNLKAQYAGLKPEIEAKVLEVIASQQFVLGPEVEALEKE
                     VAALSGTARAVGVSSGTDAILVALMALEVGPGDAVLTTPFTFFATAGTIARLGARPVF
                     ADIDPVTFNLDPLRARDALAAFRKREPGVRVKAVLPVHLYGQCADMDAFLALGRDEGL
                     AVVEDAAQAIGADYPSGSGVRKAGACGQAGTLSFYPTKNLGGFGDGGMVLTNDPGLGR
                     RLRKLRTHGETERYFYDEVGGNFRLDALQAAVLRVKLRHLPEWQKKRRALAAAYNRKF
                     AETGLQAEGAVVPPAAVHEFSGAADYHTFHQYVIRAADRDGLRDFLRENGVGTAVFYP
                     LPLHLQKCFASLGYRPGDLPVAEKASKEVLALPIYAELRTDEQDYVVDKIREFYSR"
     gene            27285..28292
                     /locus_tag="PKV64_00580"
     CDS             27285..28292
                     /locus_tag="PKV64_00580"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPN15600.1"
                     /translation="MTATFITGGFGHLGRHIVKAVSDLNPDGGIRLLVRTPRPTPSGL
                     EGLPGVRMIRGDLNRPETYAAGLEGAASVIHAAALVSFKPSAAPDLYRSNVLGTRRLL
                     EAAAAAGCRNFIYISSIAAVGRPVPPAPADESLIPDLERKRRIDPYGYSKRLGELELE
                     RAAGRIRRIILNPSVFIGPGSRRFDAVLRGLKYLPAVPMIPARNSFVDVRDVARAAVS
                     ALSAGSDGERYIVNGYDVDMPEFTRVLLKAMGLRRPVKVVPGSVLRATDGLIRALTAL
                     GLNPGLRPLGTVNVDRAYATDKIRREMGWAPAYSLEQSLRDTAGLGPSFPSGPVGFQP
                     F"
     gene            complement(28254..29378)
                     /locus_tag="PKV64_00585"
     CDS             complement(28254..29378)
                     /locus_tag="PKV64_00585"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HPN15601.1"
                     /translation="MTRILRSAGAILALLVFPPWISPPSAFPAAAPENDFIRGVWLHP
                     GQFGPDETAAATAMGAVFEAYVRAGIDSVFIMVKSPSGHVYFRSSAGVPDPAYAYDFF
                     GLFLREARRRNLTVHPWFCVFPESAGIGEAGRRPEWLVQGLNGETAGSVNPAIPEARA
                     YERGLMLELLRNYPEADWIHLDYIRYPCEPTEPFYGFDKRSRDLFKAETGADPLELKA
                     KDTGNMMWAEWLRWNSRRVTLFIQELRRETQTLGRTVRISAAVFPDAAASKVLIGQDW
                     AEWARSGLVDMLCPMLYTNEVELFETYAREAVALRTDRCRVCLGIGIGTSHNRNTPEG
                     MAGQIEIGRKAGADGVVFFSGNSLGEPFLKRLEADRAGRE"
     gene            complement(29428..30072)
                     /locus_tag="PKV64_00590"
     CDS             complement(29428..30072)
                     /locus_tag="PKV64_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPN15602.1"
                     /translation="MKKRLLLVAAAVLVSAAAAPAEFYFGAAGIYSAPLDQPYSSRFG
                     AGISAGWIWSPRFAVELGLGWRPSRVTGSLEGLSDGTLRVFPLEASLRARLPLGSGWV
                     LGGEAGAGWAFHSFSLSDNLRAAWEALGISIKESADGGPVVHLGIGIEYLLTPRWSLE
                     AGLRYTRSRTKGTWSITDEAGGLVQSGTLEKLKLDAVSLTFGLKIALFGAGESQ"
     gene            complement(30069..31655)
                     /locus_tag="PKV64_00595"
     CDS             complement(30069..31655)
                     /locus_tag="PKV64_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HPN15603.1"
                     /translation="MRKSGDAIELEIFRNLFISIAEEMGAVLGRTGLSPNIKERKDYS
                     CAVFNRRGETLAQGSHIPVHLGAMPLSVQAALGALTFEDGDLVLVNDPYRGGTHLPDI
                     TAISPVFVRGRPAFFTANRAHHSDVGGMSPGSMPLATEIFQEGLILPPLKLISRGRLN
                     EDVLNLILANVRTPEERRGDLLAQIAACEKGRGRVLEAVEKFGLDQVERYAGLIQDYT
                     ETFLRKTLRTISPGTYACADFLDNDGITEEPVRISVRITVRGGRAKIDFRGSSPQVKG
                     GVNANAAVTISAVLYVFRSLIEEDIPVNTGLLRPLEIVAPPGLIISARFPAATAAGNV
                     ETSQRIVDVLLGALAKAMPGRIPAASQGTMNNIALGGIDPVRGRSFAYYETLGGGMGA
                     SRERPGLSGVHTHMTNSLNTPLESLEKYLPLRIRAYSLRPGSGGAGANRGGDGLVREY
                     EFGVPTRVTIMSERRVFPPYGLAGGGPGRRGRNILISKGRRTVLGSKSDVTVQAGDVI
                     RIETPGGGGYGLPARKKESR"
     gene            complement(31652..33646)
                     /locus_tag="PKV64_00600"
     CDS             complement(31652..33646)
                     /locus_tag="PKV64_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HPN15604.1"
                     /translation="MRIVRIGVDTGGTFTDFVISENGRLSTKKLPSTPDDPSRAILEG
                     LREYLEAGPGLVLIHGTTVATNALLERKGSKIALVTTKGFEDILSIGRQTRRLLYSLK
                     GEPRIPLVQRDLCFGIAERTTADGTVERTPPPSAVRTLARTLVRRGVEAAAVSFINAY
                     ADGTNEEAVAAELERRGLLVCVSSRLLPEYREYERTSTTAVNAFLMPVLDRYLESLEG
                     GLDKASLFIMQSNEGYISPRKARKEPIRTALSGPAGGVVGSLELARAAGFPEFISFDM
                     GGTSTDVSLVDGDIRRTNENTVGDFPIRLPIIDIHSVGAGGGSIAHIDSGGSLRVGPR
                     SAGADPGPACYGRGEDPTVTDADLVLGRIDPDYFLGGRMVIEPERSRAAIGRLAVRIR
                     KTTLETAQGIVDIANANMEKAIRVISLERGFDPREFALLSFGGAGGMHAADLAASLRM
                     KAVLAPQNAGVLSAFGLLLADSIKDYSRSVLKTVEKADPAVLERGFQEMEKRGRRDLA
                     EDGFALQAMFFERSVDLRYLGQSYEINVPFPPGGFKSLAEEFHRLHEKTYAYRHARGV
                     VEIVTLRVKARGAAGKIRLRKIPPRPGASAGDAARKIQAIHYRGRAHKGPVYDRSRLS
                     PGLRFDGPALVVDEGSTTFLPPGFALRVDGYHNLIIRKKE"
     gene            complement(33648..33863)
                     /locus_tag="PKV64_00605"
     CDS             complement(33648..33863)
                     /locus_tag="PKV64_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPN15605.1"
                     /translation="MPLYEFQCKKCRKRFEALVRLGREAEVVCASCGSSDVRKLVSGF
                     GIGGGSSRLKESSGCSSCKGGTCSTCH"
     gene            complement(33905..35566)
                     /locus_tag="PKV64_00610"
     CDS             complement(33905..35566)
                     /locus_tag="PKV64_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPN15606.1"
                     /translation="MKIRRAAKGISTILVFAAGSALVLWAAARPNPSSGETAPPPADL
                     SGYTLPLKSTPSKIDSPLRRLIQTETAAVRTVVEIRDDGRRLPSPDDTRFFVRRIEEL
                     GGTVERTSGRLIQALLPPESIAFLAMSPGISRLRLPSLPQPAAVLSEGVARSGAYSWF
                     DLPSYRSTANPVKIAVLDLGFSGYAALIGGELPAGIIVRSFRADEDPEAGLVHGTACA
                     EIVHDMAPDALLYLVNFQTDVELGAAVDYLIAENVDVISCSLVWPGAGAGDGTGPICE
                     IVKRCAARGILWVNAAGDDALSHWQADFTDANNDSFQDFASGDEILQWAVAAGGETRA
                     VLTWDDWGEWNGSSYDAPTRDFDLLLWRWTGAAWELLEFCDDWQTGEAGQKPLEQTSL
                     WKTGKATYYGISILKWNAAGAPRLRLIVQGNAEAVEYAVPGGSLGIPADAPGAVAVGA
                     TDFETDVLQAWSSRGPTADGRIKPDLTAFSRVSTAVLGPRAFSGTSAAAPHVAGALGL
                     LMAKTPFAPGQLRTILEERAKDLGEIGPDNLFGRGRLNLYKKTGS"
     gene            35716..37809
                     /gene="fusA"
                     /locus_tag="PKV64_00615"
     CDS             35716..37809
                     /gene="fusA"
                     /locus_tag="PKV64_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPN15607.1"
                     /translation="MKAYTPDKIRNLALTGHGAAGKTSLAAAMLFNAGVTNRLTKVDK
                     GNTITDFEPEEIERKISISSALCVVEHGETKINVVDTPGYSNFLWDTRACLRAVDAAV
                     VLVDAVAGVEVGTEKVWDMLEEYKLPRVLIVNKMDRENAHFGPALESIRQFFGRQAIP
                     IQIPLGEEKNFQGVVDLLSMKAYVFEKDESGKFTEAPVPAEIKDEADKRFRELVEMIA
                     EGDEKLMETYLDKGELSPEEILAGLKAGIAARQIFPVFAAAGGANIGVQPFCDRVAGL
                     FPSPLDRPEIEVTINGRPGTLKPSPDEPFAALVFKTISDPYTGRISMMRVFSGKMPSD
                     GAAVNPGKETDEKFSGFFFLQGKEQAPAGAAVAGDILATAKLKATMTGDTLAAKGSGL
                     MIPPITFAEPAISFAIEPKTRADEDRISQASQRLVEEDPTIRFERDPDTNQLLISGGG
                     ELHVRIVTDKLKKRYNVDIDLKPPKVSYKETIKGRADVQGRHKKQTGGRGQFGDVWIK
                     MEPLPRGKDFEFEDKIFGGAIPRNFIPSVEKGLIEAKKKGVLAGFPVVDFKVILYDGS
                     YHEVDSSDIAFKIAASKAFKLAMQQAKPTILEPVMNVEVYTPEAYMGDIMGNLNGRRG
                     RVSGMEQKGQMRILKATVPMSEMLDFEPTLTSITGGRGSFLMEYSHMEEVPAHLQQKI
                     IAEAVKEGRIKTEEE"
     gene            37915..39150
                     /locus_tag="PKV64_00620"
     CDS             37915..39150
                     /locus_tag="PKV64_00620"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HPN15608.1"
                     /translation="MMKSILPRLARVTAILLAAPLFGQEIDPSFLLHIGDPRFKDLTV
                     EVKAGEIVSLETGAPVSFETMIEEMRAVPFVFLGETHDSLPMHELQARILRALSDRDP
                     ALAVGLEMYPVAFQEPLSRWSAGLLDEDAFLREGRWYTTWNQNFAYYRPIFALIKERR
                     LPLYALNAPREIITRIRRAGWDALSDEEKAIVPKPDLANEEHRRLMRTIFSSEEMPEA
                     MKGAGMEMMFEGLYRGQAAWDSVMAANAVKARGGGERKVVVLVGSGHLLFNLGLNRRT
                     VEMSGLPGKTVVAVEIPRGKSSLTVSRTLADYIVGLEAEERRAYPSIGLAFKKFAGLD
                     NPVIERTPIDGAARGRDFEKGDVVLSVDGRKFTDIDEMRILLAGFGWGDEVRFRLLRA
                     GTEKDVVLKMEETLPAPGK"
     gene            complement(39161..40078)
                     /locus_tag="PKV64_00625"
     CDS             complement(39161..40078)
                     /locus_tag="PKV64_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPN15609.1"
                     /translation="MTRFILTAVVLSSTVFLGGQTPPADPSPKPAATQPGPMTVGRVS
                     GGLYLVKGGAGANAAFYVGEESVIVVDAKMTEEAVRGMLAEIARVTDRPVKFLILTHS
                     DGDHVNGLPGFPGGLTIISSAATKAEMAAAFQEERSAGRRAFLPAETYEARLELIFRV
                     PRGKTASVTLLELGPAHTGGDTIVVFQEEKAAFIGDLAFVGRDPLIHRSKGGTAAGYV
                     AALKKMIELDGVETYLSGHADPLAKADLRALLSDLEEIIARVKALAAGGKSLEEVKAA
                     LLPPPLPDAPPSRWPSFVENAYLELTEKK"
     gene            complement(40147..41013)
                     /gene="sucD"
                     /locus_tag="PKV64_00630"
     CDS             complement(40147..41013)
                     /gene="sucD"
                     /locus_tag="PKV64_00630"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019571136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HPN15610.1"
                     /translation="MSILVGRDSRVIVQGFTGKEGSFHAQRMIEYGTRLVGGVSPGKG
                     GRRHLGRPVFDTVRQARAASGADVSVLFVPPFAAADAIMEAAEAGISLIVAVTEGIPV
                     LDVLRVRAFLRRTSSLLIGPNTPGVITPGRAKVGIMPGSIHRPGRIGILSRSGTLTYE
                     AVRQISDVGLGQSTAVGIGGDAVTGLSFVDGLKLFRADPETDAVVLIGEIGGTAEEDA
                     ARLIRDQYPKPVFAYIAGLTSPPERRMGHAGAIIEGRAGRAGDKARALSEAGAIVIPL
                     PSEIGATVAARL"
     gene            complement(41010..42188)
                     /gene="sucC"
                     /locus_tag="PKV64_00635"
     CDS             complement(41010..42188)
                     /gene="sucC"
                     /locus_tag="PKV64_00635"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HPN15611.1"
                     /translation="MRLIEHRSKALLSRYGIPLPNGELVETSARAAAAAERLGGRVVL
                     KAQVPAGGRGQAGGVTFALGPEDARAKAESLFKTPLVTAQTGGKGLPVRTILVEEAAA
                     LRSEFYLGAAVDRSEGRILLLASTEGGAGLEETARTKPGAVVRETVDPFAGLRPFQAR
                     RLAFALGLSGETRTQAEAIMDGLVRAFLQNDATLAEINPLGLTEDDRFLALDAKIVLD
                     DNALFRHPELRPAAEDPDADPAEAAAARSGLSYVRLGGDIGCLVNGAGLAMATADLIQ
                     AAGGRPADFLDIGGGVSEDAVKTGFEIVLSDAGLRAVLVNVFGGIVRCDVIARGLLRA
                     AGEKGVRVPFVVRFQGTNAEEGRNLLAASGLEYESAETMGEAAARAVAAARRTGEGRA
                     "
     gene            complement(42241..43473)
                     /locus_tag="PKV64_00640"
     CDS             complement(42241..43473)
                     /locus_tag="PKV64_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPN15612.1"
                     /translation="MDSGSPRVILIGVDGASWNFMNPLIKRGKLPNFERLMKEGSSGV
                     LRTINPTKSSVIWTSIATGKTMVKHGIVDWTYIKDNDITVPYSQSARRVKAFWNILGE
                     LGLNVGVVNWFVTFPPEEVNGYMVSPAFATGADDPLAPGAAITYPPELLGELDFVHGE
                     NYESLRTKESIPDLGAGAPKGSLAAHFYKYLLADRAVESASLYLYKKHPVNVFATYFR
                     ILDVTSHFAVSHVDPELLAKANEESKKDGVSKATRGQVDLAFSRVLEPVYAYMDRVVG
                     RILGLADDRTTVIVVSDHGFMLGKDGMGYNHFKMPTLPHGVVFLKGPAFKAGYKIEKA
                     HIYDILPTLLYLLGQPAAEDMDGKIMTEAFKDEFLKTHSVKTVKTYEGGAARKIAPDN
                     KEIEKKMLEDLRTLGYIK"
     gene            complement(43679..44611)
                     /gene="mdh"
                     /locus_tag="PKV64_00645"
     CDS             complement(43679..44611)
                     /gene="mdh"
                     /locus_tag="PKV64_00645"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HPN15613.1"
                     /translation="MRKKIALIGGGQIGQILAMIAAQKELGDIVILDIPEFENPAKGK
                     ALDLMEMAPHGNYDAFITGTSDYKDIAGADVVIVTAGRPRQAGMTREDLLAVNLKIIS
                     TVAAGVKQYAPNAFCIIITNPLDAMVYSFYKLTGFPRNRIAGMAGTLDTARWRAFIAM
                     ELGVSVADVAGTVLGGHGPDMVPLPRLTTVGGVPLTEIATKEQIDRLALRTREAGTEI
                     VKLFGKGSAFFSPAWSAIVMAESYLRDKRRILPCAALCQGEYGVNGLFIGVPAMISAN
                     GVEKIYEIKLTEDENALLQKTIAGVKKTTEETKL"
     gene            44821..45036
                     /locus_tag="PKV64_00650"
     CDS             44821..45036
                     /locus_tag="PKV64_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15614.1"
                     /translation="MIIKVNGRTIELFAGASVGDAIRRHSREAWRNVRGKRAFVFDAW
                     GNEIALDGALSEGDEIFVRSSGAGERS"
     gene            45036..46505
                     /locus_tag="PKV64_00655"
     CDS             45036..46505
                     /locus_tag="PKV64_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPN15615.1"
                     /translation="MKRMIRLVPAAGLVLAGILASGSGSPAAAEIPPSGETRIVIFHS
                     NDVHGKIENFAKVAAILKKERASGAEVFYLCAGDNFTGNPVVDRFEPKGEPVLEIYNR
                     LGLDALCLGNHEFDYGLDVLESFTARARFPVVCANARDDSGVLPHLRSSVVLTGRKGT
                     KIVLFGLIQIEAGNGLPSTLPERVAGLRFEEPFLKASELKDLRGDAPLFIGLTHLGLG
                     QDRKLAGQMPGLDAIIGGHSHTRVDPAATVNGVLVAQAGSDNRFLGRVDLVLRDGRVA
                     EKKGCLIDLDREKKEDREIKRLIEEFNENPALARVLASAPFDITGKEALGSLMTDAWR
                     RELGLDIAFQNNGGIRLRRLSRKITVKDVYALDPFENQVVVMIMTTDEIRNLIRISFE
                     KGEGIDLQVSGLVYTVRIDAERRVQDVQLIGQDGRPLQEDRTYKVGFSSYIPSAYDFA
                     HRDPGKSLGITTADALINYLEKRPRLSGYQNVQRARIRH"
     gene            46871..49435
                     /gene="acnB"
                     /locus_tag="PKV64_00660"
     CDS             46871..49435
                     /gene="acnB"
                     /locus_tag="PKV64_00660"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HPN15616.1"
                     /translation="MLDHYFKHADERRALGIPPLPLNPEDTAEVCRLLEAPPAGEADR
                     LLSLLRDRVSPGVDPSAGVKAAWLADVARGRKTSPVVPPEQAVFLLGTMIGGYNVGPL
                     VEFLGVPALAQTAAEALKGTILVYAAFDRVAALAATNTAAAGVLKSWAEGEWFLRRPA
                     FPKSLTLTVYKVDGEINTDDFSPAKQAATRPDIPLHALAMGGTRFPDGAAAIARFRAE
                     NKRVAFVGDVVGTGSSRKSACNSVMWHIGEDIPFVPNKRRGGVVLGGLIAPIFFNTTE
                     DSGGLPIQNVDVTRMNTGESIVLDLEKGTIMSAAGALLSTFELKPATIKDEFRAGGRL
                     NLIIGRALTQKARAALRLPEAPFFIPVRNPEAKKGQPFSQAQKIVGRACGVDGMLPGS
                     ACEPKMTTVGSQDTTGPMTADELKELACLEFQTDLFMQSFCHTAAYPKPADVKMHRFL
                     PGFITERKGVSLRPGDGVIHSWLNRLLLPDTMGTGGDSHTRFPIGLSFPAGSGLVAFA
                     GALGFMPLDMPESVLVRFKGKLNPGLTLRDAVNAIPYFAIRRGLLTVAKKGKVNVFNG
                     RILEMEGLPDLTVDQAYELTDASAERSAAGAVIALSRDQVARNLRSNIALMTKMIEQG
                     YGHAETLRRRIAAVEAWLAEPALIERDEKAVDGGAYAAVLEIDLADIPEPLLACPNDP
                     DDVKLLSKVAGDKIDEVFIGSCMTNIGHFRAAGRIFDKAGCPPAKIWLTPPTKMDETR
                     LKMEGYYAIYSQVGARTEIPGCSLCMGNQARVAPKATILSTSTRNFDDRMGDGARVYL
                     GSAELAAVAALKGALPSPAEYFETMKAKVLPAAEELYAPLDFAGTKDFTLGYAGDR"
     gene            49441..50199
                     /locus_tag="PKV64_00665"
     CDS             49441..50199
                     /locus_tag="PKV64_00665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPN15617.1"
                     /translation="MITLNGQRTLITGGSRGIGRAAAILFAEAGSDVAFNYVRDEKAA
                     AEVKEAVESRGRLCLVRKADLARREDAAALAGAVADAWGGVDILVNNHGIWTYLEMGE
                     GNVEAWKTTMEVNLDGVYYLTDAVVPQMKARSKGCIINVGSTAGIRGEAHHSHYAATK
                     GALNALTKSWSSELAPFGIRVNCVAPGWVDTDMSAGSFADPAFRESIRQSIPLKRIPP
                     AEDIAGPILFLASDLARHITGAIINVNGGAVLCG"
     gene            50274..51185
                     /locus_tag="PKV64_00670"
     CDS             50274..51185
                     /locus_tag="PKV64_00670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPN15618.1"
                     /translation="MGKKHLSLIVIPHTTKSPRTWSFSRRTVKAATVCGIGLAVLMTA
                     VTVDYVRVRLSRRSYHSLVAENAKQKEELRVYGTKVGELERKLNGFREYAGKLNVFAG
                     IKSPEMLTNPGVGGGQSAFPPDAAQVIPPPGNPPPSIASVQDLTRTADDLEKNLDALL
                     SHFEQLQGQFATRPSIAPVIGYRSSGFGWRLDPFGSGRRAFHSGLDIATVHGNPVVAT
                     ADGAVLRVAYDGALGNNIIINHGNGITTLYGHLSKALIKPGARVKRGDTIGLVGRTGR
                     ALGDHVHYEVRLNGSAVNPNLYILDEF"
     gene            complement(51189..52130)
                     /gene="fmt"
                     /locus_tag="PKV64_00675"
     CDS             complement(51189..52130)
                     /gene="fmt"
                     /locus_tag="PKV64_00675"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPN15619.1"
                     /translation="MRVVFFGSPEAAVPSFQALLEAGHEIPLVIAQPDRPVGRGRRLL
                     PGPVKRFALDRGIPVAQPEKIRLDPEILDRLRAAAPDIQVVVAYGQIIPMTIVALPPR
                     GTVNVHFSLLPRFRGAAPVAAAIAAGETRTGVTIFSLNEKMDEGDILAEEETPIGSLE
                     TAGELEARLASLGAGLLLRTLDRIDEIIPRSQDHSLATLAPKIRKEDGRIEWTRSAVE
                     IDRRVRALSPKPAAFVRLRGSRLIILAGRPVEGVATPAHQGPPGTVTSLSKEGITVAC
                     GGGTRYLITRLHPESRAEMDAHAFVLGGGIQAGDLLE"
     gene            complement(52139..52648)
                     /gene="def"
                     /locus_tag="PKV64_00680"
     CDS             complement(52139..52648)
                     /gene="def"
                     /locus_tag="PKV64_00680"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPN15620.1"
                     /translation="MSLRPIITIGHKTLAAKSAPVTVIDDGVRTLARDMIETLHAAPG
                     LGLSAPQVNVSVRLIVLDLSIGENPDDLIIMINPEITSQEGQIIREEGCLSVPEVFEK
                     VTRPQKVTVQGLDLEGKERVLEARDLLARVFCHEIDHLDGKLFVDRLSPLKRSLIRKK
                     FRKAAAAKP"
     gene            complement(52809..54833)
                     /locus_tag="PKV64_00685"
     CDS             complement(52809..54833)
                     /locus_tag="PKV64_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HPN15621.1"
                     /translation="MSHQPFVPEKTDMKEFTLRALLIGLVMTVVLGAANAYLGLRAGM
                     TIAATYPAAVIGMAVLRLMKGSILEENFARTVGSIGESVAAGAIFTIPAFLIAGVWLK
                     FDTFDAYIKTTALLFVGGLLGILFVTFLRRVMVTDPELGFPESVAAAEIHKAGQHGSS
                     SAKHLFRAMGAGSFFYALDMFGIYAASKDFLFKIGKIGQSFVRLGTEEASTKVTAGGM
                     VSVSGPGIFPAYLGVGYIIGPRLASLNFAGGLLAWGLFAPVLMFFLGPQLIDMIYGAG
                     AVVPAEDWGGLVTNVWKFIIRPIAVGGMLVGAGYTLFKMRKNLSAGVKRSFSDVKKAT
                     GQTHVVSRLEKDLNIKIVLGGLALTFICMILVYRVFAGAFFPALIAAVVMGVAGFFFA
                     AVSGNLVGTIGSSNNPISGLTLSTLIIAALLMVVVGAAGASGVAAVLGVAAVVCVSSA
                     VAGEMLQDLKVGHILGGTPWKMEVGNVIGIFLAAAVMYFPMMLLHGAYTFGSRELAAP
                     QAGLMAALAKGIVGGEMAWPLVIAGMLMGFAMILLQVKSPMLVSVGMYLPLETTFAIF
                     VGGMIKGLADKAVAKRRLNPAQKARAENVGILIAAGLIAGEGLTGLFKALWKILFLQN
                     AIKFDVPRLFSAPSYWLGLLAIAAMAWYLIYVPIRNAGKADEPPPPSAVM"
     gene            55019..56050
                     /locus_tag="PKV64_00690"
     CDS             55019..56050
                     /locus_tag="PKV64_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPN15622.1"
                     /translation="MSLYRWFKNRLFCDLAIDLGTANTLVYMKGKGIIIQEPSIVVLN
                     KQTGKVEAVGQRAKEMLGKTPANILAIRPMRDGVIADFEIAEKMLDFFIKEATNNRGF
                     LLRPRIVIGIPTGITQVERRAVKDVAMRAKATEVFLVEQPMSAAVGAELPISEPTGNM
                     VVDIGGGTTDIAVISMDGIVFNHSIRIAGNEMDEAILQYLKKKYNLLIGERSAEQVKI
                     QIGSAYPLDQPLTMEIKGRDLREGIPKTIVIDDQEIREALEEVVGAIINAVRIALEKT
                     PPELSADIIDRGVILTGGGALLKNLDKRIREETQLPVFITDDPLTAVVLGAGKMLDDI
                     DLLRKIAIP"
     gene            56066..56905
                     /gene="mreC"
                     /locus_tag="PKV64_00695"
     CDS             56066..56905
                     /gene="mreC"
                     /locus_tag="PKV64_00695"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPN15623.1"
                     /translation="MPRFLKERKNGLVLAGLMFAQAVLLSFQIPLGEEASYIERAAFA
                     VFAPIQHGIRSALRGGGDFLGRYLYLRRVEEKNRRYRDELFRLRQENALLRNSLERLE
                     DRAAADAFLNRLDRAFVLADVIGVDALQPFKSIVINRGTRHGVRLQMPVVDARGRLVG
                     RIIAPIDSGEATVQLITDNLSAVSVRGLEHPVSGILSGDPASGRAWMIYVPASDESLV
                     ENEALATTGFDRVFPPGLPAGTIVSIGTDGSLFKKIAVQPAFDFRDLEIVAVLTGPSG
                     GRE"
     gene            56902..57384
                     /gene="mreD"
                     /locus_tag="PKV64_00700"
     CDS             56902..57384
                     /gene="mreD"
                     /locus_tag="PKV64_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPN15624.1"
                     /translation="MRNWIDGAIAAAAAFLAFSLLRPLSASADLVVDVFSVAVLVFGT
                     HKGEAPGAVFGAACGLIVDAFSLGVFGLAGLSLTLGGFLAGYVSRKINIQPFFRMFAF
                     FAVLAAGTFAGWAGLSALIGRSPLPWSGGLLLLRPAVSASAAAMINLGIRRWKARRER
                     "
     gene            57374..59185
                     /gene="mrdA"
                     /locus_tag="PKV64_00705"
     CDS             57374..59185
                     /gene="mrdA"
                     /locus_tag="PKV64_00705"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPN15625.1"
                     /translation="MSDKGIYEDFSLVRRRARRIFVIVAVLCGLALAVYWKIQILDYK
                     KYESLAVANRTREIILPAPRAILTDRTGDIILADNRAAFKASFIRENTRDEAASIARV
                     ASLLELDPSVVASRIEKYKSWPAFRPIVVKDRLTFEEVARIEARKNDLPELIVETEPR
                     RLYPRQSFCSHVLGYLQEADANDLKERARELRPGDMVGKMGIEQGYDRRIAGREGRVI
                     DVVDSLGRKQSELERVDPVPGKKLALTIDYDLQAKAEELLAGREGAIVVLDPSNGEIL
                     ALASFPTFDPNKFITRFTPEEWQSFVNNPDNPLLNRALQGQYSPGSLFKPVMGIAALD
                     SRTIGLETTFYCGGGIEIYGALRRCWNEAGHGALSLRDAIRYSCNVFFYQLGRRIPID
                     TIARYAEMMGLGRKTGVDIPGEKEGLVPSTRWKKTTRNEEWYPGETISVAIGQGPLQV
                     TPLQMAVLTARLANRGRGIRPHLVLGASEEPPGDSALNVRPEVFEQVIEGMWRSVNEG
                     GTGQGARVEGFAACGKTGSTQTISRETAERIGTGAKAAKTHSWFTGFAPRAQPRLVVT
                     VLVEYGGGGGATAAPIAGKLFSLYKSNHDRPRSTQGN"
     gene            59154..60245
                     /gene="rodA"
                     /locus_tag="PKV64_00710"
     CDS             59154..60245
                     /gene="rodA"
                     /locus_tag="PKV64_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPN15626.1"
                     /translation="MIDRVQLKEIDWVLTALMVANTLAGILLIYSASADSGGGYAWKQ
                     LLWMAAGLIVFFLTISIDYKAWMAFAPYIYALFLLFMAGLFLFGRTVSGSRSWIRIAF
                     MGGQPSELAKVAVLLMLARVFSEYRAPYASRGVIALSAGLVGVPMGLILLQPDFGTAM
                     CFLPILFGALVLAGLTKKTVAAMLIGAVVLGAAGWNFFLKDYQKKRIEMMINPAQDPR
                     GAGYHVLQSQIAIGSGGLTGKGYLKGPQSRLRFLPARHTDFIFAVLAEDFGFAGVFAV
                     LAAYFAMLARMFSSVGLSRDRAGIYIAFLAAGLISFQFLVNVFMIVGWLPITGIPIPL
                     LSYGGSSLLSTYFALGLVVNVKMRRFANV"
     gene            60238..62751
                     /locus_tag="PKV64_00715"
     CDS             60238..62751
                     /locus_tag="PKV64_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HPN15627.1"
                     /translation="MFDPERLFPRIEKPGRYTGGEWNSIRKDPARTSVAVALAFPDVY
                     EIGMSYPGQKILYDILNARPDLRAERVFAPWPDMEDALRASGIPLFSLENRTPLDAFD
                     VVGFSLLYELNYSNVLTMLDLGRIPLLAADRPDDAPLVVGGGPAVFNPEPAAAFFDAF
                     LAGDGEEAFPEIIDLWIRLKARGERRAGILRALAGIRGVYVPSLYETRPAGTPPLLVV
                     RPASGDAPFPVEKRVLPSFDRAPFPSGIVVPNVQSVFDRIAVEVARGCPQKCRFCQAT
                     SLYAPFRLREAESVVETALDGVVRTGYEDASLFALSVSDYPYLSETVRALMSELEPRR
                     ASLSLSALRPRGLSAGIAGEITKVRKTGFTLVAEAGTDRLRRVINKDVSDQDLMEAAA
                     NAFRRGWRLLKIYVMIGLPTETREDVEAIAGLAGRLTLLGRELTGGSPQINLSVSSFI
                     PKPHTPFQWAAMDGPETLQEKQRLLKDLLFKRRNVKVKTHPVENSLLEAVFSRGDRRL
                     GGVLLRAWRAGARFDSWGDRFRFDVWRRAFEEEGVDPAAYVSSLDPEAALPWDHMASG
                     VAKEFFRREFLKALAAEPTPSCLDASCGACRGCAFIGAIGKKPPAPPRFTPPAAPPPE
                     RADGGGEEVVRYEAVYIKEGRLRFIGHNDLVNVIQRTFRRAGIPAAYSEGFHPMMRMA
                     FPPPLALGMEGRDERLEFKTRAVLAPAVCADALNSSAPDGLRFRGLRPLERDAPPLSR
                     RLRGLVYSVDLGHLAVGDVLAGPGPDGGTSEFVRRKIEAAGLPAGGPEIRLDPDRNRL
                     VMTFRFDPAKAPRPQDVLSAVFGLTNPASVLVRDAVLVD"
     gene            62863..64014
                     /locus_tag="PKV64_00720"
     CDS             62863..64014
                     /locus_tag="PKV64_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPN15628.1"
                     /translation="MMDFTLSEEQQALQEMAREFAQKELRPVAAKYDQSHEFAYDVME
                     KAFQAGFLTGNIPEAYGGGGLSNLDLAVVSEELAAGCAGLFTSMMACSLATAPLILFG
                     TEEQKRKFLEPLCRKMSLAAFCLTEREAGSDAGSVQTRAVRDGDVYVLNGAKCFITNG
                     GVASLYTVFANTAPAKGARGVTAFLVPRDTPGITVGKVEDKMGQRASNTAEIFFEDVR
                     VPAANILGRERHGFIIAMKTFDQTRASVGAASVGVARAAMEAAVAYAKTRRQFGKPIA
                     LYQAIQFKIAQMAIEIQAARHLVYHAAWLADRGLPNGSESAMAKTFASDTAFAAANEA
                     IQIHGGYGYMRDYPVEKYLRDAKLFQIYEGTNEIQRMVIAHEVIGPVKE"
     gene            complement(64134..64523)
                     /locus_tag="PKV64_00725"
     CDS             complement(64134..64523)
                     /locus_tag="PKV64_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPN15629.1"
                     /translation="MAKAEPNVVPLCDVLLVLLIIFMVITPMVQKGIDVRLPEVASEG
                     SGGQPAGLIVCTLNKDLTVNINNENYPGLQNAGDELRRLYSTRSDKTIFLRADVKLPF
                     SKVVDLIDVCKGAGVDTLGLVPEMIDE"
     gene            64646..66394
                     /locus_tag="PKV64_00730"
     CDS             64646..66394
                     /locus_tag="PKV64_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HPN15630.1"
                     /translation="MNVLAVLIAAAAAFVLAGRFYSRFIARRLGEDPSRPTPAVALRD
                     DLDFVPTKVPVVFAHHFSAIAGAGPILGPTMAVLYGFVPAWLWVLLGGIFIGAVHDFT
                     TLFVSLREDGRSVAEIARRTLGERGFHLFIAFTLIMIVLVTSSFLSATSISLTSLWPL
                     EKIGVREGETFLRTVVVDGVPMGRIGGIASMSVIVITLCSPLLGFLIHRKGVKMTVAY
                     PMAAAIGVTSVFLGIAFPVTVSASAWMIIISVYVLFAAGLPVWLILQPRDFINVQILY
                     AGIALMAVSLVRTGLAGVRVTMPGFNLAEGVRALGSIWPMMFCMIACGAISGFHALVA
                     GGTTSKQLSRESHARIVGYNGMLLESLLAVCVLLAIGAFLSFGDYRSIVWPTEPGIKS
                     NPILGFSLAAGHLFNRGLGIPTALGTVFGILLVEGFVVTTLDAAVRLNRYLFEELWEI
                     LFRKVPGFLRKHWINAGISVLLMWCLAASNTFTLLWPVFGTANQLLAAMSLLAVSAWL
                     MLRRRRAWFTEIPAVFMLATTIASLAILFRQYLRTKNYVLAAADAALFVLAAGVVVLT
                     VRLLRKERRRTAAEKA"
     gene            complement(66413..66808)
                     /locus_tag="PKV64_00735"
     CDS             complement(66413..66808)
                     /locus_tag="PKV64_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPN15631.1"
                     /translation="MKDVKVESELEVITPNEPGILGRVLGTLANAGVNLRAMCVTSHP
                     EGGRFRLLTSDNKKAEAALRTLGYKVKAQSVLTVVISDRIGAGAEVGALLGNAVIDIH
                     TAYATAAGKDAILLVLQTNANKKAYETLR"
     gene            66994..68472
                     /locus_tag="PKV64_00740"
     CDS             66994..68472
                     /locus_tag="PKV64_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPN15632.1"
                     /translation="MKSKKLTVFLIVLLAFGLVTSYAETKKWRRTGSNTFVRIKGKVP
                     TAEVMKTLADKYAGDIKIGMDQVGQGDLYEPVLQALKDGIFTEASLPAGEKFAWMFFR
                     LGGKVKVWTDVEWAGKKPLDVFLFDVKTPTKLYTYAMPRPCGNLALYKAVDVIPPAPP
                     ATCLLVVNPMKANLNEPVVIDMCGTQNAASMTVEIFDAQGVKIADRNLTPADCKWQTS
                     FDKAGSYVFKGAAVNADGVASSNPCEVTAYINAPPTCKLWTSCLPCEDYVGKPITFDA
                     NGSTDPDGQLVKAVFEITDETGNVIDSYTANAKPFTWQKVFTKAGKYGVNVSVFDDMG
                     AASSTADPCHLTFDVTQKRFFWVAEIGGLLARGTYTGFFFGRVGMLWGVVPDLMDIVL
                     TMGPAIPSQGDPWKVFFMGNVTANLHLGPSVYVGAGLGYTTKEQETRKSGIDLVGQFG
                     VNIFNHYTSAGSIFAEARIPVLTSDRPVDDHYKLLLGFRYIF"
     gene            68534..69409
                     /gene="rfbD"
                     /locus_tag="PKV64_00745"
     CDS             68534..69409
                     /gene="rfbD"
                     /locus_tag="PKV64_00745"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPN15633.1"
                     /translation="MKIALIGADGQLGTDLRTALKGHEVVPLFWPEFDVTRAEETAGK
                     LSLIGPDAIVNTAAFHRVDECEDRPEAAFAVNAIAVRDLARTARELDAVLVHFSTDYV
                     FDGEKRTPYVEQDLPGPLSVYAASKLAGEHFVRAYAGRAFVIRTCGLYGKAGCREKGR
                     HFVAAMLQAAESGRKEIRVVDDQVVTPTTTAELAARIAELLRTDAYGLYHMTAEGSCS
                     WADFARAVFELSGRDPSIIVSVDSAALGAKARRPAYSVLENARMKETGLTPFSFWRDA
                     LAAYLKDASPPSPAR"
     gene            complement(69575..69865)
                     /locus_tag="PKV64_00750"
     CDS             complement(69575..69865)
                     /locus_tag="PKV64_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HPN15634.1"
                     /translation="MSSRLSREKINYLSTRILEVLSKNDTVEYLDEPNEVRLSIVRAI
                     EEEMRLYDQLDKKATDKIQSQKKPIEEGSREWEILYRKYYNEELSKLGKLSE"
     gene            complement(69862..70140)
                     /locus_tag="PKV64_00755"
     CDS             complement(69862..70140)
                     /locus_tag="PKV64_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HPN15635.1"
                     /translation="MRLNKNQIDHLAGMILRNLLKEGKILAENKERVLEEITVQITEE
                     FQKEDRLDVEVREILTKHMEKIRRENLEYQTMFRMIKTKLARERNIVL"
     gene            complement(70171..71073)
                     /gene="truB"
                     /locus_tag="PKV64_00760"
     CDS             complement(70171..71073)
                     /gene="truB"
                     /locus_tag="PKV64_00760"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPN15636.1"
                     /translation="MDGLLLLRKPSGPTSHDCVVRLRKILGEKRIGHFGTLDPFAEGL
                     LLAGIGKATRLFPFFGTIGKTYEGVIRLGQATDTYDRTGTALGPAVDSLPAEEDVLRA
                     MARFRGDLIQLAPPFSAKKLAGKPLYVYARGGAEVERRPAAVRVDRFELRSYAPPDID
                     FEVSCSAGTYVRSLAHDLGADLGCGAHLHRLVRTAMGKYRLDEAKTPEEIEAAAAAGR
                     FEAFLIPPEGLLEDLPAVSVTAAGRALVRNGRAIGLFGSAVAVPPEPAPAPGAVLRLF
                     DPENRLIALARIKENEAAPFLVLI"
     gene            complement(71082..>71770)
                     /locus_tag="PKV64_00765"
     CDS             complement(71082..>71770)
                     /locus_tag="PKV64_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HPN15637.1"
                     /translation="DGYFKINIDHHYSNSHYADINWVDAEAPAVAELVLDLCETAGLA
                     LTPSIANHLYCAIVSDTGSFQFSNTTARAFAACHKLVLAGARPIETSEALFHNNRPEK
                     IILLGRVLSTLRLNAAGTIAQIGMFRKDLIELNLRQVDTEDITTLARSIMGVRVVLFF
                     KEMEPDVFRVSIRSKGEAHAAGIAEHFGGGGHAHAAGFTVYGPFPIISAEVMASVENL
                     LDAPASSGSR"
BASE COUNT        13392 a        22332 c        23171 g        12875 t
ORIGIN      
        1 gccgttcagc gctttttggt tttggatttc gccttggctt taaccttggc tttgggcttg
       61 acctttttca tcggcttttt ggccgctttg ggcttcgcca tggccttggc tttggccgcg
      121 gctttgggtt tgacccttgg cttcggcttg accttaggtt tcgccttggc tttccccttg
      181 ggtttcgctt tgggtttgat ctttccccgg ggcttggccg cgactccggt cttggccgaa
      241 ggcacggctc cagcctcggc ccggattttt tcaaggttcg ccttggccgg cgggaccttg
      301 tcgatgaagg cggagacgac gccacaggga tagaggtcgc acagggcgca attcgcgacg
      361 ttcttttcca agccgcattt ccggatttcg cactcgaagc agtagccgat gtgcggcccc
      421 tccaagacgg tgcagcccgc gcaatccacc atctccggag tgatctccgc gccgtattgc
      481 ttcttccact cttcggccgt tttctcccga agcgccgcgt cccccgtttg gtggacgacg
      541 taagccgggc aaacggaaca atcaagcccg caaatcgcga tgttcatggc tggcctccct
      601 ttccgcaagt atccgccgat ccttcgctgg cgtcaatagg ggattcatcc cctgattatt
      661 gttcgggacc gaccgtgacc caggaggccc gggccggatc catgagccga cggatctcgg
      721 cgttgacctc atccagggtc agggaataga gctcatcgcg gaaatgagcg aaaaaatccg
      781 ctcccagtcc catcgattca taccagccca gggtcgtcgc ccgggctgcc ttggtctcgt
      841 tggcccggag gaagccggac cacagaatgg cccggccggc ttccagctcc tcggcggtta
      901 agccgcgctc ctggaagtcc cggagaacct cggccagggc ttcccgggcc gaggcggttt
      961 ttggggcgtc cgtctcgagg taagcttcga acagccccgc cctccggaaa aagaaggctt
     1021 gggcaaagac gttgtaggcc agcttttgat cgacccgcag cgtccacagg cgcgagccga
     1081 cgccccggcc gagggcgttt tcaatgaggg agatccggac gtagtcccgg cggctcatcg
     1141 acggcaggat aaagccgcag gagacgacgc tctgttgttg cttttttatg acgatcctcg
     1201 gcgggtaggg gggatccttg aggacgagag cggccggggg gggcggcggg gctcccgggc
     1261 ggagggaggc gaaatggcgc tcgatcaaag cgccgagggc gtcgggcgcc aggtcggaga
     1321 cggcgacaat ggtgatgttg ccggcgacga acagccgctc gtagaaatcc cgagcgtccc
     1381 ggactctcaa ggctcggagc gattcctccg tcccgagcgt ctcgccggca taggcggaat
     1441 cgccgaagaa tgtctcccgc tggacgaggt gggattcgta ccgggcgtcg tcgatctgaa
     1501 tgcggcgctg gtgctccatc gattcgacga gccggttcat ccggatgtcc gaaaagatcg
     1561 gccggttgaa aatcttagag atcgtcgcca gcatcggctc ggcgaactcc gttagaaatt
     1621 ccaggtggat gacggcgtcg tcgtcgcgcg cgcccatccc gctcttgagg gctttcaccg
     1681 tgaagtcgcg gctggtcgtc tcgtcgggga tgttgacgga cagccgggcg gtgagatggg
     1741 cgattccagc ccggccgccg ggttcggctg aagccccgcc gccgatgacg atttcgacga
     1801 ccgtgatcgc cgaggaggcg tccgtctggg tgatgaccgt gaggccgttg ggcagaacct
     1861 tcttcaggat gggagcctgc gctccggcga atgcgggccc ggcggcgaat ccgccgaggg
     1921 cggcgaggag ggcggccgcc ggaaaccgga accaggggcg ggcgggccgc cggccggagg
     1981 atgcgcgcct tgggaaggat ttcatttctt tcgggggacg atggagacga tgacgaaggc
     2041 gtcgcggttg aaagacgcgc cggcggcctt ccggaggtcg cccgacgtca cggccgcgat
     2101 cctctccagg tagtcgattt ccgactccgt gccggcgagg agcatatggg cggccatcga
     2161 ggacgccagg ccgagaccgc tttcccaagc ctgctggccg agaaagcgga ttttattctt
     2221 ggcgctctcc agcagatcga aggcggccgc ccggtcctcg atcagaacat cctgagggga
     2281 aaaattctcc ctgcggactc ccttcagaaa ggtgatcgtc tcgcgcttgg cgaccgagat
     2341 cttggatggt tccagggtga tataggccac gaaagctccg gcccgcttca aggcgatgta
     2401 gcttaaatgc gccgagttga ctcccgcccg gcggctgttc ttgagggcga cggtcatcat
     2461 cgggtggatg ccctgaccga ggatctgggt gaggacgtcg gccgcgtact gtcccgggtc
     2521 gttgtaatca gggccgagga cgccgacgaa aaggtaagct tcgctgatgt cgagctcccg
     2581 ctcgatctca accgtcttcg aaggagggcg ggcgaattcc ggcggaggcg gaggatcctc
     2641 cccccgggga acgtcggcga agacctcccg gacgcgggct tccatttcgt cgaccggcag
     2701 gtttccgaca atggccaaga cggtgttgtt gggaacgaac agcctcttgt agaagcgctc
     2761 gatatccccg gccgtgagat cggccaagca tccggggtcg ccgtagatgg gccgtccata
     2821 gggatggccg gccaggagat tttggaagac gagcgcctgg cccaggcgga acggatcatc
     2881 ctcgatctgc cggatctcct cgccgatgac ttctttttcg gcgttcagcc cgtcttcctc
     2941 gattttcatt cggaagagga tttccttctg gtttttcagg ccgaagagga agtgctcggc
     3001 gggcagggtg aggtcgaaga acgccgcgtc ctggccggtg tgggcattga agacggctcc
     3061 gcggctacgg atatcccggg cgatctcgct tccggaacga acgtccgttc cccggaaaag
     3121 gatgtagtgc tcgagcagat ggatcaagcc ggacgtttcg tcggtttcgt ccttggaacc
     3181 cgcgttgact ccggcgacga tgttcaccag gggaacggaa ttccgctcga gcaggaagac
     3241 gcgcaggccg ttgtccaggg tgaattgttt ttgcggggag gatgccggcc ggacgagcgg
     3301 ggggaaaagc aggaccgtcg cggcgccggc cagaaggagg agggccgccc cttggatggc
     3361 cgccggccgc cgttccgcct tcctttcgat catgtctccg ctcgggttta ttttatcctt
     3421 tccttgcgga gagaaaaagc aatctttgtg ccagccgggg cttgtcatcc ttccaaagtc
     3481 tcgcccgccg aagccgcctt cccgccggag gagcggattg ttgaacgccc ggccccgcgg
     3541 cgggcgttct caaatccgcc tttcattaaa cccgtttttc gtttttatct tcagcggatc
     3601 agacggcggt tgtcttgact cgcgaatcgg gcgggttggc cggcggacgg agccgcggac
     3661 cgaaacgggg aggttccaga atccgcggaa agcagggttt tcgagcgctt ggggagtggg
     3721 aaaaaagaat ttccgcccgc cgtcggcgcc cggggcggaa gggggccggg aagatttttt
     3781 acaagggacg ggttttttgt aaaattcctg ccggggcggg cccgaagaga ggggactttc
     3841 aagaggaggc gccggcgcgt ttccggagag ggaggctcct gtgttaaaat cggcctgaga
     3901 tcgaggcgaa acgcatgaag cttgaccgca aagcccgcat cctggtcatc gacgacgagg
     3961 agaacatccg caagtcgctg aagatgattt tggaatacga cgggtacatc ttttacgagg
     4021 cggccgacgg cgaatccggc cttcaggcga tcgacgagat tcccggcctc gacctcgtcc
     4081 tcctggacat ccatcttccg ggccgggacg ggctggagat cctcaaggag atccgcaacc
     4141 gccccttccc gcccgaggtg gtcatgatct ccggacaggg gacggtccag acggcggtcg
     4201 aggcgaccaa gctcggcgcg ttcgaattcc tggaaaagcc gctccaccgg gaccgcatcc
     4261 tcctgaccct gcggaacgcg ctggatcgca acaagctggc ccgggaaaac ctggatctgc
     4321 ggcgcaaggc ggagaaacgg tacgagctcg tcggcaacca ccccctgatg aagaagctct
     4381 ggcgggacat tcaggcggtc gctccgacca acgccacggt cctcatcttc ggggaaagcg
     4441 gcacgggcaa ggagctgatc gcccgggccg tccacgcccg gagcctccgg gccaaggaag
     4501 ccttcattca agtcaattgc gcggctatcc cggaggagct catcgaatcg gagctcttcg
     4561 gccacgagaa aggggctttc accggggcga ccgagaaaaa gccgggcaag ttcgagctgg
     4621 ccgacggagg gacgatcttc ctggacgagg tcggcgacat gagcctcaag acccaatcca
     4681 aggtcctccg cgtcctggag gaaggcgagg tccagaaggt cggatcaaac aagatctcca
     4741 aggtcgacgt gagggtgatc gcggccacca acaaagacct caaggccgag atcgagaaga
     4801 atctcttccg cagcgacctg tatttccggc tcaatgtcct gcccctgtct tcgcccccgc
     4861 tgcgcgagaa gcgcgaggac attcccgtcc tgatcgatta tttcgcccgg atgttcgccg
     4921 aggagaacaa cttcaaggtc aagacgttct cccccgaggc ggtcgaggtg atgacccgct
     4981 gctcctggaa gggaaacgtc cgggagctta ggaacgtggt cgagcggctg ttgatcatga
     5041 ccgagggcga ccggatcgag aagagcgacc tgccggagca gatccgcggg gagaagggcg
     5101 tcgttcttcc cgatgcgtcc aaggtccagt cgctcaagga tttccgcgac ttggccgagc
     5161 gcgaattcct cctggccaag ctcgaggcca acggctggaa catcagccgg acggcccggg
     5221 agattgacac gccgcgctcg aacctttata aaaagcttga gcagtacgga atcaagatca
     5281 cggccggagt ggacgagacg gtcgctcccg aatccgtcaa ggaagagggg gaggaaagtc
     5341 cggactccgc agggcagggt ggtcgctaac gacgaccccg ggcgaccgga gggaaagtgc
     5401 cacagaaaac acaccgcccg ccagggcaag ggtgaaatgg cgaggtaaga gctcaccgcc
     5461 ccggtggtga catcgggggc agggcaaacc ccacccggag caaggccaaa cgtgcccccc
     5521 atgggatcgc ccgtcccggg ggcgggtcgg ccgctggatc ccgcgggcaa ccgcggggct
     5581 agacaaatga ccgtccccgg cgcttcggcg ccgggaacag aatccggctt atgggccgct
     5641 ccggccgtgt ctttcacacg cctccggcgt gtaaaaaaat acggccgtga gttctgccct
     5701 acatgaccgc ttcccttagc ttgtcttcgt tcccgtggtc tctccgattt caaatcgtga
     5761 acgtcacggc acacgcgccg gcgattttct cattaaataa tttgatattt caataacgta
     5821 taatgagatt cggcttggtc gtcgatcttc agggacagcg cggataagac attcgttttt
     5881 ttaccgtaat ataatgacat caggcgatga gggtaggtca cttcatcgag ctttttctcc
     5941 atgatgaaat tcccatccaa tgaatatagt tgaacgatgg gattcaatag atcgatttcg
     6001 cggtcataat tcatatacac gatgccgata taacgatctg taacaaatat tttccgaatc
     6061 atagaatatt tgtgaagaac tttataatac ttcttcaaat tccgctcttt ttttgcttga
     6121 atcagttcct ttgatgccac cggcgccgta tataatgacg ttttattccc aaaccggtac
     6181 tgtttattcg tcttcgtgtc cacccgtatg acgcttaaca gcccttccca tacggcatag
     6241 acttgatctc catgagaatc acaatacgag cgaacgccca tgcttgcttt ttctgccaat
     6301 tgaggctgac ccgcgttggg agagatattg aataaatcct ggatcgtcaa taaaaacgaa
     6361 tgtttctttt gtcgcggata ggagagatag atttcatacc atttgccggc gggatccgct
     6421 ttcgaccccg aaaataaata atgatccgga aaaatcctgg cgtcacagca ataaccgaag
     6481 aattcttctc ctaatttaat ttcgagatcc tcattgacat gataaacaag gatacgatat
     6541 ttctcagaat tcgaaatcaa tagcttattg tcggaataat caaaaatcgt cgggtaagaa
     6601 aattcatccg gtccgttccc ccgtcttccg aagattttga cgaatcgtcc gtttttatcg
     6661 aatattttga aattgccggc ccgcgtgtcg cagatgataa aggaaccgtt gggcaggacc
     6721 gcgattccat catccgcttc tatcaagcaa tcttgctgag agacgggaat tttatcgatg
     6781 agacgaatat cttgcccgaa aacgaaaggg ccggcgatca tgagaggaga taataaattg
     6841 ataagcgttt tcttcatcgt tcacgctctc atatgacgag atgaagaggg agcggacttg
     6901 atgtccgctc ccccgggcac tctaattaat ttttgtcgta gcgcctccag caatcaacga
     6961 atcttatttc aaagcgtaga tgatcagcga ttgcaggtct ttccccccga gaaaatagat
     7021 atttccatag gaatcgacgg cgacgggatg ccagggaacg cgggccccat cggcatcctt
     7081 gatattaacg gttgtcggaa catattgatt tccttttttc tcgaccacgc ggaaaccctc
     7141 cgcgccctga aaccagatat ttcccgagtg cagtaaaaaa tgatctcttt cggcccaatc
     7201 cgtttctccc aaacggtttc ccgccaaatc gaaatatcgg atcagcttca tttcaggcaa
     7261 ggcttgaaaa acaattccgt tcgtcgaatc aaattgcggg gaagaggcga aataatcccc
     7321 gtttctttcg aggatggcct ccactgtcaa tccttgattc ccgaaggggg aaaaggaatt
     7381 cacatggttt ccgtcgaaat cgaggaggtg aagagagtcg tttctaaagc cttcttcact
     7441 cgcgatgccg ttgaccaaga tgagatccct cggcctgaga atcctcagat gggcgataaa
     7501 agagaatcgt tcgcccaggt tgatttcctt caacttctcc ccggtggatc cgttgtacca
     7561 agccaacgga agacggaact tgccttcttc cagtcttccc gaaaaaacgc aaacagagtt
     7621 gccgtagccg tcgatatcga acttgataat ctctccttca ttccggcggg gagctcttaa
     7681 aacgaccttt cccgcccgat cgagtttgag gaacgagtcg tttccccgct gcgtgatgaa
     7741 tacgtttccc gcggaatccg aggcggcgaa aacgagatga gcctgatgag gtttgagaaa
     7801 gaagccgagg tcgatttctc gttcgagaat caagttttgg gaatgagcga aacaaaggac
     7861 catcggcaaa aacgaaacga ataaaaatcg ttttttcatt tcccgtctcc ttgcttcaat
     7921 tgataattcg aaagtggaga atatccgtcg caaaaccctg ttatgcaccg ctgcaaccac
     7981 cattgattcc cctcgttgtc cgtacagacg atataaacgg cttgataaaa acaagacgtt
     8041 ttgcaaggcc agcaattttc acatcgccaa tcataggtgt tacagggcgc gaaccaataa
     8101 cccacgccgt aagtatttgc acaagatgtc tccccatttc catttttcac ctgtatattc
     8161 atatataagc aaatcatccg atttctcaag ggaaataagc cgataaaaaa gataatatga
     8221 tcgattattt ttttatttgc atgtttttaa ttgaggaaga tcccccccgt gatagatagg
     8281 cgtatttatt tatcacccgc gacggcctga tcccaaactg ggaacgaaaa cggccgctga
     8341 aggaaagggg gcggtagccg aggtcgagcg cgatttcttt tattgacttg tccgacgaaa
     8401 gaattttcca caaggaataa caaaagcgat tattgatgac aaaacggcat aaagagacgc
     8461 cggtcaactc tttgaatgtt gatgaggtcc aagacggact tcgatgaatg agcttggaaa
     8521 tgccggcaag atcatgattc cgccatggat tctcgacgac gtctttctga atgagcagga
     8581 ctttatggag aggatccgag ggatgaagcc gcccttggcc ccatccataa taacgcgatc
     8641 tttcgcaggt ttccttcatc tcccgctgcc ggggtgacaa gacgctgtcg tcgaagaacg
     8701 tgagaataaa acttttcgaa aagcccgcgg ctttcgaaaa aatcgccggc ctcaggacga
     8761 caatgggggt gttcaacctc tgaagagaaa taaaagaacg aagacaattc cgcgtcaaac
     8821 aagaataatc acaaacatga aagacaagcg aaaaaccgct ttgagacggg aagcgatttc
     8881 tggattccac gactctcaac gaggcgggaa aggggatgag tctattgatg aaatccggct
     8941 tgtcccgttt gctgataagt acgagaacct tgccgcggtt gcgagcggaa tttcttctca
     9001 tcaaccgttc cgcctccctc taaataaaag tataacaaac ggaaatgcat cgatcaattt
     9061 atatcattga ggaatcgagg gatcgccgcc ctcgtgacca atggttatta taacggcctc
     9121 acgttccacc gcgccgtccc cggcttcctc atccagggag gccagcccgc ggaccgacga
     9181 gtacgcggga gacggcccgt tcaggcgaga cgagacgggc cgggcatcga acgcgtgcgg
     9241 aaccgtcggc gtctccaccc gcggccgcga cgccggcgac gcccggattt ccatcaacac
     9301 cgtcgacaac gaccgcctga accgcgaata ccgcgtcttc gcccgggtcg tttccgggat
     9361 ggacgtcgtc gacaggatcc tcgagggagc cgtcatcgcg cgggtggaac tggttttcga
     9421 cgggcccgga atccgtccgg cggccgcctc ggccgggccg gcctccccgc cgaatcaata
     9481 atggagccgg acgacgaccg gcgtgtcgcc ggtttggggg agcaggacga agaggctgcc
     9541 gtaatcatcg tcggcgatga tttcctcgga cgggtggccg aggcgccgca tcaattccgg
     9601 cagcgtgttg gaatggccga tcacgaggac cgtgtcgccg gcatggcggg tccggagcct
     9661 ttccgccagc cccgccgcgt cgccggcggc gtatgtctga accttcaatc ctaatgcggc
     9721 ggccgccggc tcggccgtca gccgggctct ccggtattcg ctggcataga cggccgtgat
     9781 tcccagggga gcgacgagga cggccagggc ttcggcccgg gcccggcccg cctccgacag
     9841 ggcggagtct ttggaggagt cggctttttc cgcgtgacgg accaggatga ccgcttgctg
     9901 ggcggcggcc ggcccggccg ccgcggccgc gaggaccagg acaaagacgg ccgtccggaa
     9961 gccttttttc ccacggaccg cccgggatag aagcggggcg cggtcgttca tgaaacgaat
    10021 atatcgaccg ccggccccgc ctgtcaacgg cctgaatcgg agcgtcaagt cggcgtgaaa
    10081 actcccgctc atgcgcctcg gcttcagggc ttccgcgcat gatgccccgt caccgcccgg
    10141 tgaaaacgtc ttctggggtg gagtgccggg ttgttttgag gggggcatta tgaatcccgg
    10201 gcaacccgat ttacaaacgc cgtcgggatg gggtaacatg gaggccgcgc aatccgcgaa
    10261 agaatccaac cgtccaggag gcccgctcat gaacgacatc gaaaaagcct tgatgcatgc
    10321 gctcttcgtc catctccacc acaatatcga caaggccatc gatttcaagg ccatcgatgc
    10381 cttgaataaa aaagtcggca aactgttccc cggctcgctt gtcgtcggcc cgggcgacga
    10441 cgccgccgcc ttcaagtttc ccggcacgcc gggcatgttc gtcggcaaaa tggagagcca
    10501 ttgctctccc tgcgttcccc ggccgtacga cgcggcggcc accggggccg gcggatccat
    10561 gcgggacgtg gtggccatgg gcgcccggcc gtttttcctg atgaacttca tcgggaccaa
    10621 acccctggac gagaaggtca tcgtcggccc gtgcggcttc aaggggaaat gcacctgcgg
    10681 gcagtgccgg gagatgacca gcggagagcg ggtcaacttg atggtcaagg gattgaagga
    10741 catgtgccgg gccatggatg tcttcatcgt cggcggcggg ttctccacgt cgttttcgga
    10801 tatcgtcccg gccgtcgtcg tcgcggtgat gggacgcctt gtcacgcccc ggccgttgac
    10861 caagccggcg aaggccgccg gcagccagat catcctgatc ggccggacgg gaagcgacgg
    10921 caacgacact ctctaccggg ccgggctggt ccgggaaatg aagccggccg tcgcccagtt
    10981 ccgggaggaa cgcaaggtca tggaagcttc cctggccgcg ttccgcacgg ggaagatccg
    11041 ggcgtgctcc gatctcggcg cggccggcgt cggggccggc gtttgcgaat ccgcgcggta
    11101 cggcggtttc ggggcccggg tggatctttc caaggtcccg ctgaaggaaa agaacctctc
    11161 gcccgaggaa atcctgattt gcgaaaccca ggcgcggatg gtcgtccaag tcgccaagaa
    11221 ggacgtcgat ctcattttga aaacggtccg ggccaaggga gtccgggccg agcatatcgg
    11281 cgagatcaac aacgacgaca aggaagtctt tgaataccgg ggcaagaccg tggccgtgat
    11341 cccgaaccgt ccttcggccg cggacatgaa gaaactccgc gcggtctgac gggacgcggg
    11401 gaggaagacg ggctcggagg ccggtcggcc ggccgcttgc cgcttcgcct ggggaaaaat
    11461 ccggatcggg ccggagcgtc ccggcctccc ggcgctcagg tgacgagccg ggcgccggcc
    11521 gacggaagga cgacccgggg ctcgaggcgg cggccccggg cggcggcata agccgaagcc
    11581 agggaagcgg cgatcgccgg ggaccgatcc ttttcgacca ggacgacggc gctgccgccg
    11641 aatccccctc cgctcaggcg ggcgccgaga accccctgga tcttccccgc cgcctcgacc
    11701 aaggcgtcga gctcgggaca ggaattttcg aaatatttcc gggaactttc atgggattcg
    11761 aacatgaggc ggccgaaggc ggccgcgtct ccgcgcttga gggcttcggc cccggtcaag
    11821 acgcgttcgt tttccccgac gggatgggcg gctcttttcc aaacatccgg atccaggcgg
    11881 tcccggtatt tcatcatgtc cgcccgcgtc acgtcccgga gggcggcgat cccggggccg
    11941 aggatgcggc ggaacgccgc ggccgcctcc tcgcaccgcc ggcgccgttc gttgtattcc
    12001 gagtcgacca gcgtgtgctt gacccccgtg tcggcgatca ggaaagccgt cccctgcgga
    12061 agggaaacgg tgtccgcttc gagcgaacgg aaatccgtga agacgagagc ctgttcccgg
    12121 ccgaagaggc ttgagatctg gtcgagaagg ccgcagcgca cgccggcgaa ctcgtgttcg
    12181 gcccgctggc cgattttcgc cagggcaagc ttctcggcga cgattccgtg gaaagccgaa
    12241 aaggccaggc ccgaggagat ttccagggcg gccgagctgg aaagcccggc cgccaccggg
    12301 atatcgccgc agaaggttcc ttggaatccc cggggacgga acccgtatcg ttcccggagg
    12361 aacgccagga cgccgcgcgg gtagttcgac cagcgctccc ggtccgtccg tcccgggttg
    12421 acggcctgaa aacgaacgct ctcgccgaag tcggccgccc gcagggtgca ggcatcctcg
    12481 tcggccggcg agagaccgaa ggcgatcccg gcgtcgatgg ccgccgacag gacgaagccc
    12541 tcgttgtaat cggtgtggtt gcccaggatc tcgacccgcc cgggggcgta cgcgacgacc
    12601 gcgggttcga cgccgaaaac ggcctggtgc tctttgcgga gacgctgaat cagatcatcc
    12661 attccatgac tcccgacgga ttaaaagccc gcgaccgcgc cgcgaaggaa ggtcataacg
    12721 ccgcccggaa agaggccgag ctggaggacg gcgtaaagac agaagaacaa ggcgaggtag
    12781 acggggtaat tttccgtgtc gacctcgatc tcccgttctg gctccttcat gaacatgacc
    12841 cggacgatcc gcaggtagac cgcgaccgaa accaggctgg cggcgatgcc gacgagggcg
    12901 aggccgagac ggccggcccg gacggccgcg ctgaagacga agaacttcgc caaaaaaccg
    12961 ccggtcgggg gaaacccggc cagggagatg aggatcacgg tgaagatccc cgccgcccag
    13021 ggcgaccggc ggccgagtcc ggcgtagtcg tccagctcga gcgtttcgcg cttcggcccg
    13081 gccagggcga tgaccgcggc gaaggccccg acccccatga acaggtaatt ggccaggtag
    13141 aacaacagcc cggccggatc gccggagacg acggcgatca gcatgtatcc ggcgtgggcg
    13201 atgccggaat acgccaggag gcgcttgaga ttccgctggc ggagggcggc gaaattcccg
    13261 aggatcatcg tcgcgacggc ggccgcgctc aggaaggagg cgatcggccc ggccccgatc
    13321 cccgcccggg cgaaggcgaa gagaatccgg aggaggacgg cgaatccggc cgctttcggg
    13381 gccgccgcaa aaaacgccgc ggccggcgtg ggcgcgccct cggtgacgtc cggaaaatac
    13441 atatggaacg ggacgacggc gattttatag cccagcccgc agagaaccag gatcaatccg
    13501 gccgcggcca gggcgggaac cgcccccgtt ccggcgagcc cggcggccgc ggcgtcgagg
    13561 tcgagcgtgc cggaggctcc gaacagcagg gccaagccga agacgaaaaa ggccgaggcg
    13621 aaggatccgg tcaggaaata cttggcggcc gcctcggccg aaccgtcgtc ccgccggttc
    13681 agtccggtca aggcataggc ggagatggaa aacacctcca agccgaggaa aacgacgagc
    13741 aggtggcggg tggccgtcat caggatgagg ccgcagacgg ccaggagaag aaggccgtaa
    13801 tattccgccc gggggatccg gcggagaacg atgaagcgca tcccgagaag gatcgccagg
    13861 acggccgcca aaagatagag gaacgcgagg aaaagcgccg gctcatccca tcggagctgc
    13921 cccccgaacg cctcggccgg ccggccccag aggcgggcgg cggcgaatcc ggccgcggcc
    13981 gcggccgcga cggagaccgc ggcttggacg gaattcgatc ctccgccgcg gcgcgtcccc
    14041 aggagcaaaa cgagaaccgc cccgccggca aggaagagaa ggggggcgag aacgaccggt
    14101 ccgctcatcg cgctttttcc tcgccgccgc cgcttccgga gacgatcgcc ggaaaagcgg
    14161 gacggccgtc cacccggacg tatgcggtcc ggcggttttg cagggcatcg gcataccgga
    14221 cggcggtcgc ctcgatcttt ttcaggaagg tcccgggaaa gagcccgatc caaaacatca
    14281 ggaggataac ggggaggaag acggccagct cgcgtccgcc gatgtcccgg agagcggcca
    14341 cgcgcccgga ggcgacgggg ccgtgaaaga ctttccggta catgcccagg aggtaagcgg
    14401 ccgagagaat caccgtcgtg acggccaggg cggccaggat tttatccgcg gcgaacacgc
    14461 cgctcaggca aaggatctct ccggcgaagc cgttgagtcc gggaaggccg agggacgaga
    14521 gcgcggcgat gagaaacaaa gcggcgaaga cgggcatctt ccgggcgacg ccgccgaagt
    14581 cggagacgcc ccgggcccgg gtccgttcgt acaagacgcc gacgccgaag aagagcagtc
    14641 ccgtgctcag gccgtggttg atcatttgga ggatcgcgcc ttggaggctg accgtgttcc
    14701 aagccgtcac ggccagcagg ataagcccca tatggctgac gctggagtaa gccacgagcg
    14761 ccttgatgtc cttttggacg agggccatga acccgccgta aataatgccg gccacggcca
    14821 gcccggaaag gaccggagcg aggcgggcga cggcctcggg gaagagggga aaacccaagc
    14881 ggagaaatcc gtaggcgccc atcttgagga gaacggcggc caggagaacg gaagccccgg
    14941 tcggagcttc gacgtgggcg tcgggaagcc aggtgtggaa cgggaacagg gggaccttga
    15001 cggcgaaagc caggccgaag gcgagaaaga gccaggtctg ggcggaaggg gagaggacca
    15061 tccggcccaa gtcgaacatg tccagggaat atgtcccggt tgcgcgggcg taggctccgt
    15121 gaaggacgaa gatgccggcc agcatcagga ggcttccggc catcgtgaag aggacgaatt
    15181 tcgtggtcgc gtagagccgg cgttttccgc cgaagacgcc gatcaagaaa tacatcggga
    15241 tcagggccgc ttcccagaag acgtagaaga ggaagagatt aagcgagaga aagacgccga
    15301 tcatcccggt ttccaggagg agcaggaata ggagatattc cttcaaccgg tcgcggatag
    15361 acgagaccga ggcccagacg accaggggcg tgagaaaagc ggccagcagg acgagaaaga
    15421 ggctgaggcc gtcgaccccg aggtggtagc cgaggccata gcccagccag gggcttttct
    15481 cgacgaactg aggcccggcc tcgcgtccgt cgaaacggaa ggctagcagg caggcgagga
    15541 cgaagacggc aagcgtgacg gccagggcgg cggcgaagag gcgccgccgt tcggcggccg
    15601 ggatgaagat gaggagaatc gccccggcca gcgggaggaa ggtgatcagg ctcagcagcg
    15661 ggatgttcat gtcagaagag gaggactccg aggaaagcga cggccccgag gaggatggcc
    15721 agggcgtaat ccttgaccca tcccgtctgg agaagggcga gggcttttcc gagaaggccg
    15781 gcggctccgc ccgccccgtt cacggccgcg tcgatgaccc ccaggtcgaa ccggcggtaa
    15841 accgccttcg acccgcggag gaggggattg aggagggccg tctcgtaaag cgcgtcgacg
    15901 ccgaagcccc ggaggagaag atggtggaag ccgggaaagc gggctgaaac ccggccgggg
    15961 agggccggat tccggacgta gaaaagatac gcgaggaaca agccggcgag ggccgagatg
    16021 gtcgaggcgg ccagcagcgc ggcctcggcc cccggtcccg cgtgcccgga ggccggtgtt
    16081 ccggcgaccg gggcgagaaa ccggaaaaac aggttcgctc gttcgccgaa caaggccggg
    16141 aggccgacat agccggcgac gatcgaacaa aaggccagga tgaggagagg ccgggtcatg
    16201 acggccgggg attcgtggag gcgggattcg acctcggccg agaagcgcct gctcccgaaa
    16261 aaagcgagaa agaccagccg gaacatgtaa aatgccgtca gcccgccgcc ggccaggccg
    16321 aggattccgg cgaagacgtg tccgccggcg aaggcggtga ggaggatggc gtccttggag
    16381 aaaaatccgg aaagaaaggg gatgccggag atcgccaggg cgccgacgag aaaggcggga
    16441 taggtccggg gcaggagttt tcgaagcccg cccattttcc gcagatcctg ctcgccggac
    16501 agggcgtgga tgacgcttcc ggcggccagg aaaagaaggc tcttgaaaaa ggcgtgggtg
    16561 aacaggtgga aaatgccggc ggcgtaagcc cccgcgccgc agccgagaaa catgtatccc
    16621 agctgggaga tcgtggaata agccaggacc cgcttgatgt cgttttggac aagggcgagg
    16681 actccggcgt agacggcggt caaggccccg acaagcatga cgatcccgga cgccgaggaa
    16741 gaggccgcgt aaagcgggtt caaccgggcg accatgtaca ccccggccgt gaccatggtc
    16801 gcggcgtgga tcagggcgct gaccggcgtc gggccttcca tggcgtcggg cagccagaca
    16861 tagaggggga tctgggcgga ttttcccgtc gccccgatga acaggagaac ggcggccgcc
    16921 gcggccgccg aaggggcgag cgctccggag gcgaccgctt cggaaaggcc ggcgaacgac
    16981 accgttccgg ccgccgacca aaggacgagg attcccagaa ggaagccggc gtccccgatc
    17041 cggttgacga gaaaggcttt cttgccggcg tcggccgcgg ccggtttttc gaaccagtag
    17101 ccgatcagga gatatgaaca gaggccaacc ccctcccagc cgacgaacat caagaccagg
    17161 ttgtcggcca ggacgagaag gagcatggcg gacatgaaga ggttcaaaca ggcgaaatac
    17221 cgcccgaagt cgcgctcccg ggacatgtag ccggccgaat agacgtggat gagcaggccg
    17281 acgccggtga cgaccagaac catcacggcg gccaagggat cgaagcggaa agccgcttcg
    17341 gcccggaagc cgccggcttg gatccaggtg aaaagccggt ggatgcggac tccggaaccg
    17401 gaaagggccg cggcgacgga cagaacgaac gacgcggcga ccgacccgca ggccagcgtc
    17461 gagaccgacc ggcgggacag ccgccggccg agcggaaaga ggaccagggc gccgacggcc
    17521 ggggcgagag gaatgaaaac gaaggccggc atccttcaac ccctcagctt gtcgagttcg
    17581 tccgtccgga tggatttccg gcggcggtaa aggtgaagga tgatggccag gccgacggcc
    17641 gcctcggccg cggccacggt catgacgaaa aagacgatga cctgggcgtc gatccgggcc
    17701 gacccccggg ccaagccgag aaacgcgagg ttggcggcgt tcagcatgat ttcgacggcc
    17761 atgaactggg tcaggaggtc gcgcttgacg aaaaaggcga tgaccccgag gacgaacaga
    17821 acgaggctca agccgacgac ggccgccggg ctcaagactc cggctcctct tttttcccca
    17881 gcacaacggc cccgaccaaa gcggccagga tcaaaacgga cgtgatttcg aacggatagg
    17941 cgaattccgt gaaaagacgg cggccgacgt ccgcgggcgc gacgcccgga ccggaggccg
    18001 gactccggtt ggaattcgcc aggatccccc aggcggccgt tcccaattca aaaagaagga
    18061 cgaggccgat ggccgggccc aggattttcg cccgtttccg ccgctcgggg ggagccggcg
    18121 tccgcggatc gaccatcatt acgacgaaaa tgaaaagaac catgatcgcc ccggcgtaga
    18181 cgagaatctg gacggccgcg gcgaaggggg cgtcgagcag gccgaagatg ccgcccaagg
    18241 cggagaagca caggacgaga aaaagggcgt tgtgtcccgg gttgcgggaa atgatcatcg
    18301 ccgcggcggc tcccgcgctg acgacggcca gaatccaaaa cacaatatct gcggccatgg
    18361 cgggcgtaat tataaaacaa aaccggcggg atacaagacc caatccctcc gcttcttccg
    18421 aatccattcc gaacctggtt ctccatttcc cgatatccgt tttccttgat atcggaacct
    18481 ggtcaaccat ttcctttcat cagcaactga ggagtactcg ccgcctctcg agcgcccgcg
    18541 ggcgggcggg gctggatttc agaccgcgcg atgcgaccgt ttgacggaac ggccgggccg
    18601 gggtacagtg gagtcgccgc ccttccggga tgcggctgaa gcccgacaag gaggaggatc
    18661 gatgatgatc ctcgcggccc ggcccggtgg gatcgcgacg aaggcgtatc ccgacctcgc
    18721 ggcatcatcg cgccgggagg aacgcgggat gcggccgaac aggctccggc ggccgggccg
    18781 ggccggatcc ggcggcgccg ggtcgtccgg gccggatgga attcccccgg gcggggtcga
    18841 tcatcgccgg agagaggaaa cacgacatgc gcttgatgaa aagaaaaggc cggatcgcgg
    18901 ctgtgggatt cgcctttttc acgctgctct tatccgccgc ggtccttcag gccgcgacgg
    18961 ttttcaacat cgtcctggac cgcggcggcg ttcttctttc gggccgcctc cgcgtctgcg
    19021 agggagacgg gccctttccc acagtcattc tccttcacgg cctgccggga aacgaatcgg
    19081 atgttcttgg cctgggcgcc gccttggtta aaaacgggat caacgtcctg accttcaact
    19141 acagcgggac gttcgagagc caaggggaat ggagcttcgc cgacaccttg aaggacatcg
    19201 aggcggcctt ttatttcgta aaccgtcccg acaacatcct ccggttcaag atcgatgccc
    19261 ggcgcctggg cttgggcggc tacgactacg gcggcgggat ggtcctggct tatgccgccg
    19321 gccgcccgga ggtgggcgtc gttttctcca tcgccggcac ggatcacggg gaattcctcc
    19381 gggaatacgc ccggaatccc gagatgagga aaatcgtcga cgagacatac gcggccatgg
    19441 ccgccccggc cggcccggtg aggttcgccg agggcgccgt tccgcgggaa tgggcggaca
    19501 aaggcccggc ggccttggac ccgaatctcg atttcctccg aatcgccccg gccctggccg
    19561 gcaaggacat cctgcttgtc tgcgcttggg acgacgaaac gaacaaggtc gagcgcaacg
    19621 cgctgcccct ctaccgggcc ctgaaggaac gcaaggcgaa aagcgcttcg atcgccgcct
    19681 tccaggacga ccatgcgttc cgaaaatccc gggccggctt ggcccgggcg gtcggcgatt
    19741 ggctgaaaag cgtttttttc ccgaccgttt aggattcgga cgaagcgatc gccccggccg
    19801 cggggcggac ggaaaatcat tttgacaaaa aatgaatctt tgttataatg ccgatgtctg
    19861 cggggtagag cagcctggta gctcgtcggg ctcataaccc ggaggtcgtt ggttcaaatc
    19921 caacccccgc taccaaccgt ccattttttc ccttgaagat atacgaggcc gcggcctctt
    19981 tctttcggga tcatcaagcc cccgcttttc ggttttgggg aggaactccg gctgtctctt
    20041 ccggcgtctt ccgttcgctc ggctcggctc ggatcgataa gctcgtctcg cgggcggccg
    20101 gagccgaaaa ccgcgtttct tctcgagccg cctgaatacc cgcggccgga acatcccggc
    20161 cggccttccg ggatcaggcc ctgatcaccc aaagcgtccg gggccctctc agcccctggc
    20221 aaagggagcg gttttccgtt gacttgccgc tttccggcct gaatggccca gtccaatttc
    20281 aatattgata tgacatactt ccggcttgat attgactcgt tttcccgcag gcgctatgat
    20341 taaaacaaga ttatgcagat cggggaagcc ggtattttgt ctttttcccc gcgaagagga
    20401 gggcgcccga tcatgaaaac gatccgcctg cccgcggccg cggcggcctt gtggttcttt
    20461 ggaacggccg tcctcgcggc ccaggacgcc ggcctggtcg ccatcaccgt ggtcaaggga
    20521 gacacgctca acaacatctg ccgggcctac ctggtcgaac gggcggtttg gccgaaaatc
    20581 gccgttctca accgcctggc gaacccgaac ctcatctttc ccggacagaa aatcgacatc
    20641 cccctcgaat acatcggcgg attcgaggat gaagggacgg tgaccatcgc tgtcggcgag
    20701 gccgtccgaa gagcgaaaaa agacaaggaa tggacgcccc tccgtcccgg cgacgtcgtt
    20761 cattccggcg atgaactgaa aacgggcggg gacggcgggc ttttcatttc cttcgccggc
    20821 ggcaataccc tccaactgat gccggaggcc cgtttgaccg tcctggtgac gaaaaagatc
    20881 gacgatttga acatctttca gcggatactc ctcggagccg gaaaagccgt cgcccggatc
    20941 aaggacgtga ccggacggtc gtcccgcttc gaagtcaaga cgccctcggc cgtggccgcg
    21001 gcccggggga cggtcttccg cgtcggcctg gacgaaacgg agacgacgcg ggccgaaacg
    21061 ctccagggcc aaatcggcgt cgaagcccaa gccgtcctgg aaatcgtcgg ggaaggggag
    21121 gggacgatcg tccacaagga ccaagccccc caaaagcctc gaaagctccc gccgcctccc
    21181 gccgttcttg atattccggc cgaagagcaa tttcctccgc tccgcttccg gttctccaag
    21241 gtcgaggagg ccgccggtta ccgcgtcttt ctcgcccggg acgcggaggg gcgcgaaatc
    21301 atccttcagg cggccgtccg tccggaagat gccgcggtgt tcgacggcct cctgcacgag
    21361 gggacctatt atctcatcgc ccaatccatc gacgccgacg gactggaagg gccgggatcg
    21421 gcgccggtgc ccgtgcgcgt cgggattccc ttgccccccg aaccatcgtc ccccgtggag
    21481 ggcgccatgt tcacggcgac gttcatcgac gtcgtttggc cggagccgcc ccggacgggc
    21541 cggtaccatc tccaaatcgc cgaggatgag gatttcaacc gcctcgtcgt cgaccggacc
    21601 gacctggccg cggccgtgtt ccgcactccg gcgctttccc ccggattcta ccggctccgg
    21661 gcgagcgccg tttacggtca agggcgggag agcgccttta ccgcgccccg ccgtttccag
    21721 atcatcgccc ttccggacgc gcccctcctg tccgagcccg atatcgacgg gcgccgggtc
    21781 ttcttcacct gcacaaacag gggcccgggc atcgtctacc ggttccagtt ggcccgggac
    21841 gatcgttttc tcggactggt cgaggaacgc ctttctcccg atccggaaat cgccttcctc
    21901 aagccggacg aggccggcct ctatcgcgcc cgggtcgcgg ccgtcgctcc ctcgggcgtg
    21961 gaaagtccgt tttcggctgt ccggacattc cgcgttctca agggaggcgt gttccggttc
    22021 ctctgcctga ttccggcgat cgcccttctg ctggtcctcc tgatctagaa aatcccatgg
    22081 cgacgcaccg gaggtcctgg ctcccgccgt tcggatcgct cccgtttctt tctcgacgcc
    22141 ggctcatccc ggtcatcgcc ggtcttcttc ttttcttcgg agcggattat ctgggcgttt
    22201 tcgacagttg ggacatccgg gcgcatgacg cgttcttccg cgttcggagc agccggccga
    22261 cgtcggaggc gatcgttctc gtggggatcg acgcccggag cctgggcgtc ctggggcgct
    22321 ggcccctgga tcgggacgtg tacgcccgcc ttctggacaa gctggatgaa gcggccgtgg
    22381 tcggcttcga tttgatcctg gccgagccgt cggccggcga cgagcggttg gcggaggccg
    22441 tccgaaggaa cggccgggtg gtcctggctt gttatttcga cgaggcgctg aacctggttc
    22501 tcccggccgg tccgctggcc gggacgcagg tcggccacgt ccatatcgag cgcgggatcg
    22561 accagatctc ccgttctctg tttcacacca tatcccgccc tggaggtccg gttccttcct
    22621 tcagcgcggc cgtttacggg atgttccgcg gcgggcgccc ctgggcgggg cgcgaccccc
    22681 tgccggattt tcggccggac ggcgcggccg gccgcctctt ccagaccgat cattcccgga
    22741 tcaattatta cggccccccg ggcacgtttc tccggatctc cttggccgat gtcttgtcgg
    22801 gcggcgtccc ggaatcgttt ttcaagggga agatcgttct cgtcggggcg acggccgcgg
    22861 gcctccagga ccgctcggcc acgccgttca gcgggacgcg gcaggaaatg ccgggcgtgg
    22921 agatccaagc caacgccctg aacaatctcc tggacgggac ggaaatccgc gattttcccc
    22981 tccccgccgc atggctggga gcgttcttga ccgccctttt gggcttggcg cttttcatcc
    23041 ggacgccgga gcggacggcg gcctggatct gggcggccgg tttggccgga atcacggccg
    23101 cctcgtatct tctgttcgcc cggtttcacc tttggctggg gccggcgctt ttttatttca
    23161 tgacggctta tgtctttctg gccgcgtatc tcctgaagct cgccgccgcc gcccgccagc
    23221 tcgacgtcaa gtacgccagc ttggccggcc atttcgccgg cagcggggac ctcggccgcc
    23281 gcggcgggaa aaaaggattg atcggcctgt tcacccgtaa cgggatcaac gaaaaaatca
    23341 gcggactcct ggagatcgag aaatcctacg agggaacgct ggaggcggcg gtttggcgga
    23401 gaacgcgaga gctggcggaa gccttgtcga tggtcggacg gatgagcaac gagctgatcg
    23461 tccggcttgc ccgggcggtc gaatcgcgcg acgcgggcac cggaaaccac gtcgcccggg
    23521 tcgccctcta ttcccggatc atcgccgagg ccctgggccg gccgctggac tatgtcgagg
    23581 cgatcgcctt caccagctcc atccatgata tcgggaaaat cggtatcccc gacgatatcc
    23641 tgttgaaaag gggccttctc acggacgacg aaaaagagat catgcggacg cacgcccgga
    23701 tcggctacgg gatcctggcc gcctccgagc atccgcgcat ccgggaggcg gcggccgtcg
    23761 ccctcgccca tcatgagcgg tgggatggat cgggataccc ggccggcctg aaaggggagg
    23821 aaatcccgcc cgaggcgaga atcgtcgctc tctgtgatta ttacgacgcc ctgcggagcg
    23881 agcggtcgta taaaagcggc ttggaccacg atacggccgt ccggaccatc ctggaaggag
    23941 ggccggattg ttccccggcc cagttcgacc ccgaggtcct ccaggcgttc cggaaatcag
    24001 cggacaaatt cgacgacgtg ttccggaggc accgggacga cgctcccccg gccgtttttt
    24061 gacatccgcc ccggcggtct gatacatttg aaattcgaaa acgcgagcgg aaccgcccgg
    24121 atgaaggagt cctccatgtt cgacgcgaag acctacacgc aaagacgccg cctcctgaga
    24181 aaacgcctgg gatccggttt ggctctcttc ctcggcaacg aggaaagccc gatgaatttc
    24241 gccgacaacg cctacccctt ccgccaggac tcgaccttcc tttattattt cggcctggac
    24301 acgcccggct tggccgccgt cctggacgtc gacggacaaa aggagatcct cttcggcgac
    24361 gacatcgaca tcaacgccgt catctggatg ggcgacctgc cgaaaatgaa agaccgggcg
    24421 gcctcggtcg gcgtaaggtt gacacggccg gcggccgccc tggccggcgt cctccaggaa
    24481 gcggcccgga agaaacggaa aatccatttt cttccgccgt accgggccga gcatctccaa
    24541 aagctcgaat ccctcttggg cctcaagccc gcccgggttt cggccgccgt ctcgatcgat
    24601 ttcatccggg cggtcgtcgc ccagcgcgag gttaaaagcg aagcggaggt ggccgaaatc
    24661 gaagcggccc ttttcacgac ccgccgaatg tacgacgcag ccatgcggat ggcccagccc
    24721 ggcgtccggg agatggacat cgccggccgg atggaaggga tcgccgcggc cggcggccgg
    24781 ggcctcgcct tcccgatcat tctctcccgg gacgggcaga tccttcataa ccattcccac
    24841 atccaccggt tgaaaaaagg cgatttgctt gtcgtcgacg ccggagcctc gtcgccgtgt
    24901 tattacgcct ccgacatcac ccggacgatc cccgtcggtg gaaacttcac ctcgcggcaa
    24961 cttgatgttt acgagatcgt ccgggcggcc aacgaaaacg ccgtggccgc cgtccgtccg
    25021 ggccgatcct tcaaggacgt ccacgaaacg gcggccctga ccatcgcctc cggcctgaag
    25081 gagctcggat tgatgaaggg cgatgtcgag gccgccgtcc gcgagggcgc ccacgccctc
    25141 tttttccccc atggcgtcgg ccaccaattg ggcctcgatg cccatgacat ggaaaacctg
    25201 ggcgaggatt tcgtcggcta cgacgaggcg accaagcgga gcgggcaatt cggccttaaa
    25261 agcctccgcc tggccaagcc cgtcaggccc ggcttcgtcg tcaccgtcga gcccggcgtt
    25321 tatttcatct ccgccttgat cgatttatgg caggccgaaa aaaaacacgc cgatttcatt
    25381 gcctacggca agctggaatc ctggcggcgg ttcggcggaa tccgggtcga ggacgacgtc
    25441 ctcgtgaccg cggccagccg gcggatcctg gggaaagcca tacccaagac gcccgtcgac
    25501 ttggccgccg cgatcaggct ctgaaaggga agaggacgct tccggacgga aacgagcgcg
    25561 catggtcagc tggttcgttc tctgcacgaa gccccagaag gagttccaag tcgagcgcct
    25621 ttttcaggaa gcgggctttc cgttctattg cccgcgcttc aagtcgagcg ggcgaatcaa
    25681 gccgtttttc ccctgttacg tctttctccg cttcgaatac cctgaacaat tcaagctggt
    25741 gaaatacacc cggggcatcc gccgcgtcgt cggcaacgac gccggggccg tgcccctgga
    25801 ggatcgcttc atcgaggagc tccaatcccg ggagatcagc ggcttgatcg agctggagaa
    25861 atacggggtc gagccgtccc ctggcgacga gatcgaggtc gccgacggac cgtggaaggg
    25921 cctgcgcggt gtttttaaaa agagcctttc ggaccgggac cgggtgatga ttcttttgaa
    25981 ttacgtcagt taccaggggc agctgctcat cgaaaaatcc aaattgaaga aggtcctgtc
    26041 atgaatgtct cccttctcaa tctcaaggcc cagtacgccg gcctcaagcc ggaaatcgag
    26101 gccaaggtgc ttgaggtcat cgcttcccag caattcgtcc tcggtcccga ggtcgaggct
    26161 ctggaaaaag aggtggccgc cctcagcgga acggcccgag ccgtcggcgt ctcctccggc
    26221 acggacgcca tcctcgtcgc cctaatggcc ctggaggtcg ggcccggcga cgcggtgctc
    26281 acgacgccgt tcacgttttt cgcgacggcc gggacgatcg cccgtctcgg cgcccggccg
    26341 gtgttcgccg acatcgaccc ggtgacgttc aacctcgacc ccctccgggc ccgggacgcc
    26401 ttggccgcgt tccggaaaag ggagcccggc gtccgggtca aggcggtttt gccggttcac
    26461 ctctacggcc aatgcgccga catggatgcg ttcctggccc tcggccggga cgaaggcctg
    26521 gccgtcgtcg aggacgcggc ccaggccatc ggggccgatt atccctccgg gtccggcgtc
    26581 cggaaggccg gcgcttgtgg acaagccggc acgctctcgt tctacccgac caagaacctg
    26641 ggcggtttcg gggacggcgg catggttctc acgaacgatc ccggcctcgg ccgccggctg
    26701 cgcaagctcc gcacccacgg cgaaacggaa cgctattttt acgacgaagt cggaggtaat
    26761 ttccgcctcg acgccctcca agccgccgtt ctccgggtca agctccgcca tcttccggaa
    26821 tggcagaaga agcggcgggc cctggccgcg gcctacaacc gcaagttcgc cgagacgggg
    26881 cttcaggccg agggggccgt cgtcccgccg gcggccgtcc atgaattttc cggagcggcg
    26941 gattaccaca cgttccacca gtacgtgatc cgggcggccg accgggacgg tctgcgggac
    27001 tttcttcgag aaaacggagt cggaacggcc gtgttctacc cgctgccgct ccatctccag
    27061 aaatgcttcg cctcgctcgg ctaccgtccg ggcgatcttc cggtcgcgga aaaagcgtcg
    27121 aaggaagtcc tggctttgcc catctatgcc gagctgagga cggacgagca ggattatgtc
    27181 gtcgacaaga tccgcgaatt ttattcccgc tgagctccag ccgggccttt catgaccgaa
    27241 ccggccgaaa cccggcgccg ggcgaagctt cccaaagggg cgccgtgacc gcgacgttca
    27301 tcaccggcgg cttcggccat ctcggccggc atatcgtcaa ggccgtctcc gatctgaatc
    27361 cggacggcgg gatccggctt cttgtccgca cgccccgccc gacgccttcc ggcctcgagg
    27421 ggctgccggg cgtccgaatg atccggggcg atctcaaccg gccggagact tatgccgccg
    27481 gcttggaggg ggccgcctcg gtcattcacg ccgccgcctt ggtttccttc aagccgtcgg
    27541 ccgccccgga cctgtaccgg tccaatgtcc tggggacgcg ccgcctgctc gaggcggcgg
    27601 ccgccgccgg ctgccggaat ttcatctaca tcagctcgat cgcggccgtc ggccggccgg
    27661 tgcctcccgc tccggctgac gagagcctga ttcccgatct ggagcgaaag cggcggatcg
    27721 atccctacgg atacagcaag cgcctgggag agctcgagct cgagcgggcg gccggccgga
    27781 tccggcggat catcctcaat ccctcggttt tcatcggtcc cggttcgcgg cggttcgacg
    27841 ccgttctccg cggattgaag tatcttcccg cggtcccgat gatccccgcc cggaacagct
    27901 tcgtggatgt gcgcgacgtc gcccgggccg ccgtctcggc gctttcggcc ggaagcgacg
    27961 gcgagcgcta tatcgtcaac gggtacgatg tcgacatgcc ggagttcacc cgggtgcttt
    28021 taaaagccat gggcttgagg cggccggtga aggtcgttcc gggaagcgtt ctccgggcga
    28081 cggacgggtt gatccgcgcg ctcaccgccc tgggcctcaa ccccggcttg aggcccctgg
    28141 ggacggtcaa tgtcgaccgg gcctacgcga ccgataaaat ccgccgcgag atggggtggg
    28201 ctccggccta ttctctggag caatcccttc gggacacggc cggcctcggg ccgtcattcc
    28261 cttccggccc tgtcggcttc cagccgtttt aaaaacggct cgccgaggct gttcccggaa
    28321 aaaaagacga ccccgtcggc tccggccttc cggccgatct cgatctgtcc ggccatgcct
    28381 tcgggggtgt tccggttgtg cgaggttccg atcccgatcc cgagacagac tcggcaccga
    28441 tccgtccgga gggcgacggc ctcccgggcg taagtttcga acaactccac ctcgttggtg
    28501 taaagcatcg ggcaaagcat gtcgaccagg cccgagcggg cccattccgc ccagtcctgc
    28561 ccgatgagga ctttggaagc ggcggcgtcc ggaaagacgg ccgccgagat ccggaccgtt
    28621 cggccgaggg tttgcgtctc ccgccggagc tcctggatga aaagcgtcac ccggcggctg
    28681 ttccagcgca gccattcggc ccacatcatg ttcccggtgt ccttggcctt cagttcgagg
    28741 ggatcggccc cggtttcggc tttgaacagg tcccggcttc gcttgtcgaa accgtagaac
    28801 ggctcggtcg gctcgcaggg atagcgaatg taatcgagat ggatccagtc cgcctcgggg
    28861 tagttgcgga gaagctccag catcagtccc cgctcataag cccgggcttc cgggatcgcc
    28921 ggattgaccg agccggcggt ctcgccgttc agtccctgga cgagccactc cggccggcgc
    28981 ccggcttcgc cgattccggc gctctcgggg aagacgcaga accaggggtg gacggtcaag
    29041 ttccggcgcc gcgcctcgcg gaggaaaagc ccgaaaaagt cataagcgta ggcgggatcg
    29101 ggaacgccgg ccgagctccg gaaatagaca tggccggagg gggacttcac catgatgaag
    29161 acggagtcga tcccggctcg gacgtaggcc tcgaagaccg cgcccatcgc ggttgcagcc
    29221 gcggtttcgt ccggcccgaa ttggccggga tgaagccaga cgccgcggat gaaatcgttt
    29281 tccggggcgg cggccgggaa cgccgacggg ggagagatcc aaggcgggaa aacgagcagg
    29341 gcgagaatcg cgccggccga gcgaagaata cgggtcatga gggacgctcc ccggcggcgg
    29401 acggaggatt ttcctccggc gccgtcgtta ttgagattct ccggccccga aaagggcgat
    29461 cttgaggccg aacgtcagcg agacggcgtc gagcttcagc ttctccagcg ttccggattg
    29521 gacgagaccg ccggcctcgt cggtgatcga ccatgtcccc ttggtccggc tccgcgtgta
    29581 ccgaaggccg gcctccaagg accagcgggg cgtcaggagg tattcaatcc cgatcccgag
    29641 atggacgacc gggccgccgt cggctgattc cttgatggag attccgagcg cctcccaagc
    29701 ggcccggagg ttgtcgctca gcgagaatga atgaaaggcc caacccgctc ccgcttctcc
    29761 acccaggacc catccggagc ccaagggcag gcgggcccgc agggaggcct cgagcggaaa
    29821 gacgcgcagg gttccgtcgc tgagcccctc gagcgagccg gtcacgcgcg agggccgcca
    29881 gccgaggccg agctcgacgg cgaaccgggg actccagatc catccggccg agattccggc
    29941 gccgaaccgg gaggaatacg gctgatccag gggggcggag tagatccccg ccgcgccgaa
    30001 atagaattcg gccggggcgg cggcggccga aaccaggacc gcggccgcaa ccaggaggag
    30061 gcgttttttc atcgggattc ctttttccgg gccggcaggc cgtagccgcc tcccccgggc
    30121 gtttcaatgc ggatgacatc gccggcttgg accgtcacgt cgcttttcga gccgagaacg
    30181 gtccggcgac ccttggaaat gagaatattc cgcccccgcc gtccgggccc gccgccggcg
    30241 agaccgtagg gagggaaaac ccggcgctcc gacatgatcg tcacccgggt gggaacgccg
    30301 aattcgtatt cgcggacgag cccgtctccc ccgcggttgg cgcccgcgcc tccggaaccg
    30361 ggacggaggg aataggcgcg gattctcagg gggagatatt tttccaggga ttcgagaggg
    30421 gtgtttaaag aattcgtcat gtgggtatga acccctgaaa gcccgggccg ctcgcggctc
    30481 gctcccatcc cccctccgag ggtctcgtag taggcgaagc tccgtccccg gacgggatcg
    30541 atcccgccga gagcgatgtt gttcatcgtc ccctggctgg ccgcgggaat gcggccgggc
    30601 atggccttgg ccagggcgcc gagaaggacg tcgacgatcc gctgggaggt ttcgacgttc
    30661 cctgcggccg tggccgccgg aaaacgggcg ctgatgataa gccccggcgg agcgacgatc
    30721 tccaagggac ggagaagccc ggtgttgacc ggaatgtcct cctcgatgag cgaccggaag
    30781 acgtagagga cggccgaaat cgtgacggcc gcgttggcgt tcaccccgcc cttgacttgg
    30841 ggggaggaac cgcgaaaatc gattttggct cgtccgccgc ggaccgtgat ccggacggaa
    30901 atccggaccg gctcttccgt gattccgtcg ttgtccagga agtcggcgca ggcgtacgtt
    30961 cctggggaga tcgtccggag tgtttttcga aggaaggttt cggtatagtc ctggattagt
    31021 ccggcatatc gctcgacctg gtccaagccg aatttctcga ccgcctcgag gacacggcct
    31081 cgtcccttct cgcaggcggc gatctgggcc aacaggtccc cccgccgttc ctccggcgtg
    31141 cggacgttgg ccaggatgag gttgaggacg tcctcgttca atcggccgcg ggaaatgagc
    31201 ttcaagggcg ggaggatcag gccttcctgg aaaatctccg tggccagggg catgctgccg
    31261 ggggacatgc cgccgacgtc ggaatgatgg gcccggttgg ccgtgaaaaa ggccggacga
    31321 ccgcgaacga acaccgggga gatggccgtg atgtccggca gatgagtgcc gccccggtag
    31381 ggatcattga ccaggacgag gtcgccgtcc tcgaacgtca aagcgccgag ggcggcttgg
    31441 accgaaagcg gcatggctcc caagtggacc gggatgtgcg agccttgcgc cagggtctcc
    31501 ccccgccggt tgaagaccgc gcaggaataa tccttccgct ccttgatgtt gggggagagg
    31561 ccggtccgtc ccaaaaccgc gcccatctcc tcggcgatcg agatgaagag gttccggaag
    31621 atttcaagct cgatcgcgtc gccggatttt ctcattcttt tttccggatg atcagattgt
    31681 gatagccgtc cacccgcagg gcgaagcccg gcgggagaaa cgtcgtcgat ccctcgtcca
    31741 cgaccagggc gggtccgtca aaccggagtc ccggcgaaag ccgcgaccgg tcgtaaaccg
    31801 ggcccttgtg cgcccggccg cggtaatgaa tggcttggat tttccgggcg gcgtctccgg
    31861 ccgaagcccc cggacggggc gggattttac gcagacggat cttgccggcc gccccccggg
    31921 ctttcacccg gagagtcacg atttccacga caccccgggc gtgacggtag gcgtaggttt
    31981 tttcgtggag gcggtgaaac tcctcggcca gggacttgaa gccgcccgga gggaagggaa
    32041 cgttgatttc gtaggactgc ccgagatacc ggagatcgac cgaccgctcg aagaacatgg
    32101 cttgaagcgc gaacccgtcc tcggccaggt cgcgccggcc ccgcttctcc atctcctgga
    32161 agccgcgctc gaggacggcc gggtcggctt tctcgacggt cttgaggacc gagcgggagt
    32221 aatccttgat ggaatcggcc aggagaagtc cgaatgcgga gagaacgccg gcgttttggg
    32281 gggcgagaac ggctttcatc cggagggagg cggctaaatc ggccgcgtgc atgccgcccg
    32341 ccccgccgaa ggagagaagg gcgaattcgc ggggatcgaa gccgcgctcg aggctgatca
    32401 cccggatcgc tttctccatg ttggcgttgg cgatgtcgac gatcccctgg gccgtctcga
    32461 gcgtcgtttt ccggatccgg acggccaggc gtccgatcgc cgcccggctt cgctccggtt
    32521 cgatgaccat ccggccgccg aggaaataat cgggatcgat gcgtcccagg acgagatcgg
    32581 cgtcggtgac cgtagggtcc tcgccccggc cgtaacaagc cgggccgggg tcggcgccgg
    32641 cgcttcgggg gccgacgcgc agcgacccgc cggaatcgat atgggcgatc gagccgccgc
    32701 ctgccccgac cgaatgaatg tcgattatcg gaaggcggat cgggaaatcc ccgaccgtgt
    32761 tttcgttcgt ccggcggata tccccgtcga ccagggagac atcggtcgac gtgccgccca
    32821 tgtcgaagct gatgaattcg ggaaatccgg cggcccgggc cagctcgagc gagccgacga
    32881 cgcccccggc cgggccggag agagcggtcc ggatgggctc cttccgggct ttccgggggg
    32941 agatgtagcc ctcgttggac tgcatgataa acaaggaggc cttgtccaac cctccttcca
    33001 gcgattccag gtaccggtcc aggacgggca tcaggaaggc gttgaccgcg gtcgtcgacg
    33061 tgcgctcgta ttcgcggtat tccggaagca ggcgcgagga gacgcaaacg agaaggccgc
    33121 gccgctcgag ctcggcggcg accgcctcct cgtttgtccc gtcggcgtaa gcgttgatga
    33181 aggagacggc cgcggcctcg acgcctcggc ggacgagcgt ccgggcgagg gttcggacgg
    33241 cggacggcgg gggggtccgt tcgacggttc cgtcggcggt cgtccgctcg gcgatcccga
    33301 agcacaagtc ccgttggacg aggggaatcc gcggctcgcc tttcaagctg taaagaaggc
    33361 ggcgggtttg gcggccgatc gagaggatgt cctcgaatcc cttcgtcgtg acgagagcga
    33421 tcttggagcc tttccgctcg agaagggcgt tggtggccac cgtcgttccg tggatgagga
    33481 cgaggcccgg cccggcctcg aggtattccc gcagaccctc caggatggcc cgggagggat
    33541 cgtccggcgt cgaggggagc tttttcgtcg acaggcgtcc gttttcgctg atgacgaagt
    33601 cggtgaaggt cccgccggtg tcgacgccga tccggacgat tctcatttca atggcaagtg
    33661 ctgcaggtgc ctcccttgca ggaggaacag ccggaggatt ccttcagccg gctcgatccg
    33721 ccgccgatcc cgaagccgga gacgagcttc cggacgtcgg agctcccgca ggaagcgcag
    33781 accacctcgg cttcccggcc gaggcggacg agggcttcaa accgtttccg gcacttcttg
    33841 cattgaaact catacagcgg cataacggaa atattttacc ttttagcggg gcgtccgtcc
    33901 aggatcagga tccggttttc ttgtaaaggt tcaagcggcc gcggccgaaa agattgtccg
    33961 gcccgatctc gccgagatcc ttggcccgct cctccaggat cgtccggagc tggccgggag
    34021 cgaacggcgt cttggccatc aggagaccca aggcgccggc gacgtgagga gcggcggccg
    34081 aagtaccgga aaaagcccgc gggcccagga cagcggtcga gacccgggag aaggcggtca
    34141 agtcgggctt gatccggccg tcggccgtcg gtccccggga actccaggct tgaaggacat
    34201 cggtttcaaa gtcagtcgct ccgacggcga ccgccccggg cgcgtcggcc ggaatgccca
    34261 ggctcccgcc gggaacggcg tattcaacgg cctcggcgtt gccctggacg atgagccgga
    34321 ggcggggagc gccggcggcg ttccatttca ggatcgaaat cccgtagtat gtcgctttgc
    34381 cggttttcca caacgaggtc tgttcgaggg gcttttggcc ggcctcgccc gtctgccagt
    34441 cgtcgcagaa ttcgagcagc tcccacgccg cgccggtcca gcgccagagc aggaggtcga
    34501 agtcccgggt cggagcgtcg taggacgacc cgttccattc cccccaatcg tcccaggtca
    34561 aaacggcccg cgtttccccg ccggccgcga ccgcccactg aaggatttcg tcgccggagg
    34621 cgaaatcctg gaaggaatcg ttgttggcgt cggtgaaatc cgcctgccag tggctcaagg
    34681 cgtcgtctcc ggccgcgttg acccagagga tcccccgggc cgcgcatcgt ttcacgatct
    34741 cgcagatcgg cccggtgccg tcgcccgccc cggctcccgg ccagaccagg gaacacgaga
    34801 tgacgtcgac gttctcggcg atgaggtaat cgacggcggc gcccagctcg acgtcggttt
    34861 ggaaattgac caggtacagg agggcgtccg gggccatatc atggacgatc tcggcgcagg
    34921 cggtgccgtg aacgagcccg gcttccgggt cctcgtcggc ccggaaggag cggacgatga
    34981 tcccggccgg cagctcgccg ccgatgaggg cggcgtatcc ggagaatccc aagtcgagaa
    35041 cggcgatttt gacgggattc gccgtcgacc ggtaagacgg gaggtcgaac cacgaatagg
    35101 cgccgctgcg ggcgacgcct tcggacagga cggccgcggg ttgggggagg gagggaagac
    35161 ggagccggga aatcccggga gacatggcca ggaaggcgat cgactccggc ggcaggagag
    35221 cttggatcag ccgtcccgaa gtccgttcca ccgtcccgcc cagctcttcg atccggcgga
    35281 cgaagaagcg ggtatcgtcc ggagagggga gcctccggcc gtcgtcgcgg atttcgacga
    35341 ccgtccggac ggccgcggtt tcggtttgga tcagccgccg gagcggcgag tcgattttcg
    35401 acggggtgct tttcaacgga agggtgtacc ccgacaggtc cgccggcgga ggagcggtct
    35461 ccccggagga aggattcggg cgggccgcgg cccacaagac aagggccgag ccggcggcga
    35521 aaaccaaaat cgtcgaaatc ccctttgcgg cgcgccggat cttcatgtca acggccatta
    35581 tcgttttcca cggcgcggct tgtcaaccgg cttgttccaa acgacggttt tcgtgtatat
    35641 taccccttac tctttccatc cggaaatcga atcgcgggcc cggtcccttc ggaacgagcc
    35701 cgaaaaggaa caggcatgaa agcgtacacg cccgacaaaa tccgcaatct cgccctcacc
    35761 ggccatggag cggccggcaa gacctcgctg gccgcggcca tgcttttcaa cgccggggtc
    35821 accaaccggc tgaccaaggt cgacaaggga aacacgatca ccgacttcga gcccgaggaa
    35881 atcgagcgga aaatctcgat cagctcggct ctctgcgtcg tcgagcacgg cgagaccaag
    35941 atcaacgtcg tcgacacgcc cggatattcc aactttctct gggacacgcg ggcctgcctt
    36001 cgggccgtgg acgcggccgt cgtcctggtc gacgccgtgg ccggggtcga ggtcggcacg
    36061 gaaaaagtct gggatatgct cgaggaatac aagctccccc gggttttgat cgtcaacaag
    36121 atggaccggg agaacgccca tttcggcccg gccctcgaat ccatccggca gttcttcggc
    36181 cgccaggcca tccccatcca gattcccctg ggcgaggaga aaaatttcca gggcgtcgtc
    36241 gatcttctgt cgatgaaagc ctatgttttc gagaaggacg aaagcggcaa gttcaccgag
    36301 gcccccgttc cggccgagat caaggacgag gccgacaagc ggttccgcga gctcgtcgaa
    36361 atgatcgccg aaggcgacga aaaactgatg gagacctacc tggacaaggg cgagctgtcg
    36421 cccgaggaga tcctcgccgg attgaaagcc ggaatcgccg cccggcagat tttccccgtg
    36481 ttcgcggccg ccggcggggc caatatcggc gttcagccgt tctgcgaccg ggtcgccggg
    36541 cttttcccct cgcccctcga ccggccggag atcgaggtga cgatcaacgg ccgccccggg
    36601 acgctgaaac cctccccgga cgagccgttc gccgccctcg tcttcaaaac catctccgac
    36661 ccgtacacgg gccggatctc gatgatgcgc gtcttctccg ggaagatgcc ttccgacgga
    36721 gcggccgtca acccgggcaa ggaaaccgat gaaaaattct ccggattctt cttccttcag
    36781 ggaaaggagc aggcgccggc cggcgcggcc gtcgccggcg acatcctggc cacggccaag
    36841 ctgaaggcga cgatgaccgg cgacaccctg gccgccaagg ggagcggcct catgatcccg
    36901 ccgatcacct tcgccgagcc ggcgatctcg ttcgccatcg agcccaagac ccgggccgac
    36961 gaagaccgta tctcccaggc gtcccagcgc ctggtcgagg aggatccgac catccgcttc
    37021 gagcgcgatc ccgacaccaa ccagctcctg atctccggag gcggcgaact gcacgtccgg
    37081 atcgtcaccg ataagctgaa aaaacgctat aacgtcgata tcgacctcaa gccgcccaag
    37141 gtctcttata aagagacgat caaaggccgg gcggatgtcc agggccggca caagaagcag
    37201 accggagggc gcggccagtt cggcgacgtc tggatcaaga tggagccgct gccgcggggc
    37261 aaggacttcg agttcgagga caagatcttc ggcggagcga tcccccgcaa tttcatcccc
    37321 tcggtcgaga aaggcctgat cgaggccaag aaaaagggcg tcctggccgg attcccggtc
    37381 gtggatttca aggtcatcct ctatgacgga tcctaccacg aggtcgattc ctcggatatc
    37441 gcctttaaaa tcgccgcctc caaggccttc aagctggcca tgcagcaggc taagccgacg
    37501 attctcgagc cggtgatgaa cgtcgaggtt tacacgcccg aagcctacat gggcgacatc
    37561 atgggcaacc tcaacggacg ccgcggccgg gtcagcggaa tggagcagaa gggccagatg
    37621 cggattctca aggccacggt tcccatgtcc gaaatgctcg atttcgagcc gacgctgacg
    37681 tcgatcaccg gcggccgagg ctccttcctc atggaatatt cccacatgga ggaagtgccg
    37741 gcccacctcc agcagaaaat catcgccgag gcggtcaagg aaggccggat caaaacggaa
    37801 gaagaataac gaccgtggcg gccggctgaa ttttgaggct ggccgcggaa ttgaccgggc
    37861 cggtctcccg ggctatcatg accgcggttc cccgaaagac ggagggagac ggccatgatg
    37921 aaaagcatac tgccgcggct ggcgagggtg acggcgattc tcttggcggc cccgctgttc
    37981 ggacaggaga tcgatccttc ctttcttctc catatcggtg atccccggtt caaggacctg
    38041 accgtcgaag tgaaagccgg ggagatcgtt tccctggaaa cgggagcccc ggtatctttc
    38101 gagacgatga tcgaggagat gcgggccgtt cccttcgtct tcctgggaga aactcacgat
    38161 tccctgccca tgcatgagct ccaagcccga atcctccggg ccctgagcga ccgcgatccc
    38221 gccttggccg tcggcttgga gatgtatccg gtcgctttcc aggagccgct ttcgcggtgg
    38281 agcgccggcc tcctcgacga ggacgccttc ctgcgggaag gccgttggta cacgacctgg
    38341 aaccagaact tcgcctatta ccggccgatc ttcgccttga taaaggaacg ccgcctccct
    38401 ctttacgccc tcaacgcccc gcgcgagatc atcactcgga tccggcgggc cggttgggat
    38461 gcgctttcgg atgaagagaa ggcgatcgtc ccgaagcccg acctggccaa cgaggagcac
    38521 cggaggctga tgcggacgat attttcgtcg gaagaaatgc ccgaggccat gaagggggcc
    38581 gggatggaga tgatgttcga gggcttatac cgggggcagg cggcctggga ttcggtcatg
    38641 gccgccaacg cggtcaaggc ccgcggcggc ggggaacgga aggtcgtcgt cctcgtcggc
    38701 tccggccatc tccttttcaa tctcggtctc aaccggcgga ccgtggagat gagcggcctt
    38761 ccggggaaaa ccgtcgtggc cgtggaaatt ccccggggca aatcatcgct gacggtctcc
    38821 cggacgctgg ccgattacat cgtcggcctc gaggcggagg agcgccgggc gtatccttcc
    38881 atcgggctgg ccttcaaaaa gttcgccggc ttggacaacc cggtgatcga gagaaccccg
    38941 atcgacgggg cggcccgggg ccgggatttt gaaaaaggcg acgtcgtttt gtcggtggac
    39001 gggcgcaaat tcaccgacat cgatgagatg cggatcctcc tggccggttt cggctggggg
    39061 gatgaagtcc ggttcaggct gctccgggcc gggacggaaa aagacgtcgt cctcaagatg
    39121 gaagaaacgc tcccggcgcc cgggaaatga ctcgaccgag ctattttttc tcagtcagct
    39181 cgagataggc gttttcgacg aaggacggcc agcgggacgg gggggcgtcc ggcaggggag
    39241 gaggaagaag agcggccttc acttcctcga gggatttccc cccggcggcg agcgccttga
    39301 cccgggcgat tatttcctcc aggtcggaaa gaagggccct gagatcggcc ttggccaacg
    39361 ggtcggcgtg gcccgagagg taagtctcca ccccgtcgag ctcgatcatt tttttcaagg
    39421 cggcgacgta gccggccgcg gttcctccct tggaccggtg gatgagcggg tcgcggccga
    39481 caaaggccag gtccccgatg aaggcggcct tttcctcctg aaagacgacg atggtgtcgc
    39541 cgcccgtgtg agccgggccg agctcgagga gggtgaccga ggcggttttt cctctcggca
    39601 cgcggaaaat cagctcgagt cgggcttcat aagtttcggc gggaaggaag gcccggcggc
    39661 cggcggatcg ttcttcttga aaggccgcgg ccatttccgc ttttgtcgcg gcgctggaaa
    39721 tgattgtcag gcctcccgga aatcccggca gaccgttgac gtggtccccg tcgctgtgag
    39781 tgagaatgag aaatttcacc ggccggtccg tcacccgggc gatctccgcg agcatgccgc
    39841 gaaccgcctc ttccgtcatc ttggcgtcga cgacgatcac ggactcctcc ccgacgtaaa
    39901 acgcggcgtt cgctccggcc ccgcccttca cgagataaag acctccggat actcggccga
    39961 cggtcatcgg ccccggctgg gtcgcggccg gtttgggcga cggatccgcc gggggcgtct
    40021 gccccccgag aaaaacggtc gagctcagaa caacggcggt taagatgaat cgggtcatga
    40081 cggtccttcc ttttccccag tttgcctcgc cgcccggccg atgtaaagag gatgagccgg
    40141 tccggactat aaccgggcgg cgaccgtcgc cccgatctcc gagggcaggg gaatgacgat
    40201 cgccccggcc tcggacagag cccgggcctt gtctccggcc cgcccggcgc gaccctcgat
    40261 gatcgccccg gcgtgtccca tccggcgctc cggcggggag gtcaagccgg cgatataggc
    40321 gaagaccggc tttgggtatt gatcgcggat gaggcgggcg gcgtcctcct cggccgtgcc
    40381 gccgatctcg ccgatcagga cgaccgcgtc ggtttccgga tcggcccgaa agagcttcag
    40441 cccgtcgacg aagctcagtc cggtgaccgc gtcgccgccg atgccgacgg ccgtcgactg
    40501 ccccagccca acgtcggaga tctgccggac cgcctcgtag gtcagagtcc cggagcggga
    40561 gaggatcccg atccgcccgg gacggtgaat cgaccccggc atgatcccga ccttggcccg
    40621 gccgggagtg atcacgccgg gcgtgttcgg gccgatgaga agagaagaag tccggcggag
    40681 aaatgcccgg acacggagga cgtccaggac ggggatgccc tcggtgacgg ccacgatcag
    40741 gctgatgccg gcctcggccg cctccatgat cgcatcggcg gccgcgaacg gcggaacgaa
    40801 tagaaccgag acgtccgctc cggaggccgc tcgggcttgg cggaccgtgt cgaacaccgg
    40861 ccggccgaga tgccggcggc cgcccttgcc cggggagacg ccgccgacga ggcgcgttcc
    40921 gtattcgatc atccgctgag cgtgaaacga gccttccttt ccggtgaatc cttggacaat
    40981 gacccgggaa tcgcggccga ccaggatgct catgcccggc cttcccccgt ccgccgggcg
    41041 gcggcgacgg cccgggcggc ggcttcgccc atcgtttcgg cgctctcgta ttccaagccc
    41101 gaggcggcca gcagattccg gccctcctcg gcgtttgttc cctggaagcg gacgacgaac
    41161 gggacgcgga cgcctttttc cccggccgcc cggagaaggc cgcgggcgat gacgtcgcag
    41221 cggacgatgc ccccgaagac gttgactaaa acggcccgga ggccggcgtc ggagagaacg
    41281 atttcaaatc ccgtcttgac cgcgtcctcg gagactcctc cgccgatgtc gagaaagtcg
    41341 gccggccggc cgccggcggc ttggatcagg tcggcggtgg ccatggccag gcccgccccg
    41401 ttgaccaggc agccgatgtc tccgccgagg cggacgtagc tcaggccgga ccgggcggcc
    41461 gccgcttcgg ccggatcggc gtccggatcc tcggccgccg gacgaagctc gggatggcgg
    41521 aacagggcgt tgtcgtcgag aacgatcttc gcgtccaggg ccaggaagcg gtcatcctcc
    41581 gtcaagccca gaggattgat ttcagcgaga gtcgcgtcgt tttggaggaa ggctcggacg
    41641 agaccgtcca tgatcgcctc ggcttgagtc ctcgtttcgc cggaaaggcc gagggcgaag
    41701 gcgaggcgcc gggcttggaa aggccgcagg ccggcgaacg gatcgaccgt ctcccggacg
    41761 acggccccgg gcttcgtccg ggcggtttcc tcgaggccgg cgccgccctc ggtggaggcg
    41821 agaagaagaa tccggccttc ggaccggtcg accgcggcgc cgaggtaaaa ctccgagcgg
    41881 agagccgcgg cttcctcgac gagaatcgtc cggaccggca agccttttcc cccggtctga
    41941 gcggtgacga ggggcgtctt gaacagggat tcggccttgg cccgggcgtc ctccgggccg
    42001 agggcgaacg tgacgccgcc ggcttgaccg cggccgccgg ccggaacctg ggctttcagg
    42061 acgacgcggc cgccgaggcg ttcggccgcg gccgccgccc gggcggatgt ttcgacgagc
    42121 tcgccgttgg ggaggggaat gccgtatcgg gacaagaggg ccttggaccg gtgctcgatc
    42181 agtctcatcc cgggatcatc gcctcgacgc cggatcgaaa aaagcggagg gccggcggcc
    42241 tcacttgatg tagcccagcg tcctcaagtc ctcgagcatt ttcttctcga tctctttatt
    42301 gtccggagcg atctttcggg ccgctccgcc ttcataggtt ttgaccgttt tcaccgaatg
    42361 cgttttcaag aactcgtcct tgaaagcctc ggtcatgatc ttgccgtcca tgtcctcggc
    42421 cgccggctgt cccaggaggt acaggagcgt gggcagaatg tcgtagatgt gggccttctc
    42481 aatcttgtag cccgccttga aggcgggtcc cttgaggaag acgaccccgt gggggagggt
    42541 gggcatcttg aagtggttgt agcccatgcc gtccttgccc agcatgaacc cgtgatccga
    42601 gacgacgatg accgtcgtcc ggtcatcggc cagcccgagg atccgcccga cgacccggtc
    42661 catgtaggcg tagaccggct ccaggacgcg gctgaaggct aaatcgactt gaccccgggt
    42721 ggccttggag acgccgtcct ttttggactc ctcgttcgcc ttggccagga gctcggggtc
    42781 gacgtgcgac acggcgaaat ggctggtgac gtccaagatg cggaaataag tggcgaagac
    42841 gttcaccggg tgttttttat acagatagag actggccgat tcgacggccc ggtcggccag
    42901 gaggtacttg taaaagtggg cggccaggct gcccttgggg gcgcccgccc cgagatccgg
    42961 gatgctttcc ttcgtccgga gggattcgta gttttccccg tggacgaaat cgagttcgcc
    43021 gagcagttcg ggcggatagg ttatggccgc gcccggggct aagggatcgt cggcgccggt
    43081 ggcgaaggcc ggggagacca tgtagccgtt gacttcctcc gggggaaacg tgacgaacca
    43141 gttgaccacc ccgacgttca ggccgagctc gccgaggatg ttccagaacg ccttcacccg
    43201 gcgggctgat tgggagtagg gaaccgtgat gtcgttgtcc ttgatatagg tccaatcgac
    43261 gatcccgtgc ttgaccatgg ttttcccggt ggcgatggat gtccagatga ccgaggattt
    43321 cgtcgggttg atcgttctca ggacgcccga gcttccttct ttcatcaacc gttcgaaatt
    43381 cgggagtttt ccccgtttga tcaggggatt catgaaattc cagctcgcgc cgtccacccc
    43441 gatgaggatc acccgggggg agccggaatc cacccctttt ttgcggcagg acgccgaaag
    43501 gccgaagatt aacaaccctg aaaccacgag gcaaacgagc ggattgagcc ttttaaacat
    43561 agaggaatgg taatggaggc cggcgcgggt gtcaaccgcg gccggcggag gaggccgaaa
    43621 aaaagccgcc ccgccggaaa gaaggcgggg cggccgaacc gattcgggga ccgaacgcct
    43681 agagcttggt ttcctcggtg gtttttttca cgccggcgat cgttttctga aggagggcgt
    43741 tttcgtcctc ggtcagcttg atttcgtaga tcttttcgac gccgttggcg ctgatcatcg
    43801 ccggcactcc gatgaaaagc ccgttgaccc cgtattcgcc ctggcacagg gcggcgcagg
    43861 ggagaatgcg gcgcttgtcg cggagataag attcggccat gacgatcgcg ctccaagccg
    43921 ggctgaagaa agccgagccc ttgccgaaga gcttgacgat ttccgtcccg gcttcccggg
    43981 tccggagggc cagccggtcg atctgttcct tggtcgcgat ttcggtcaag ggaacgccgc
    44041 cgaccgtggt caagcggggc aggggaacca tgtccgggcc gtgtccgccg agaaccgttc
    44101 cggccacgtc ggccaccgac acgccgagct ccatggcgat gaaggcccgc caccgggcgg
    44161 tgtccagagt cccggccatt ccggcgatcc ggttgcgcgg gaatccggtc agtttgtaga
    44221 aggaataaac catcgcgtcg agcggattgg tgatgatgat gcagaaggcg ttcggggcgt
    44281 actgtttcac cccggcggcc acggtggaga tgatcttcag gttgacggcc agaagatcct
    44341 cccgggtcat gcccgcctgg cggggccggc cggcggtgac gatgacgaca tcggctccgg
    44401 cgatgtcctt gtagtcggac gttcccgtga taaaggcatc gtagttgccg tgaggggcca
    44461 tttccatgag gtcgagagct ttgcccttgg ccggattttc aaactcggga atatcgagga
    44521 tgacgatgtc tccgagttcc ttctgagcgg cgatcatcgc caggatttgt ccgatctggc
    44581 cgccgccgat cagggcgatc ttcttgcgca taacgtctct ccttcctgat attttgattc
    44641 ccgggtttca gcccggaatc gcatccgtgt ctgcttcatc ggccgcgata gtgaaatcgg
    44701 gatgggggag tttaccccga ttcgccgaaa aacacaaccg cttctccggc ttcaggttgc
    44761 cgccgacccc ggtcatcggt taaactgaaa tttcaaaaga acgaaagaag gtgcgcgctc
    44821 atgatcatca aggtgaacgg ccggacgatc gagctcttcg ccggggccag tgtcggggac
    44881 gcaatccggc gccattcccg ggaggcttgg cggaacgtcc ggggaaaacg ggccttcgtt
    44941 ttcgacgcct gggggaacga aatcgccctg gacggcgcgc tttccgaggg cgacgagatc
    45001 ttcgttcgtt cctccggcgc cggggagaga tcctgatgaa gaggatgatc cgcctcgttc
    45061 cggcggccgg gctggtcctg gccggaatcc tggcgtccgg aagcggctcc ccggccgcgg
    45121 cggagattcc accttccggg gaaacacgga tcgtcatttt ccactccaac gacgtccacg
    45181 gcaaaatcga aaatttcgcc aaagtggccg cgatcctgaa gaaggagcgg gcaagcgggg
    45241 ccgaggtgtt ctacctgtgc gccggggaca acttcaccgg caatccggtc gtcgaccggt
    45301 tcgagccgaa gggcgagccc gtcctggaaa tctacaaccg cctcggactc gacgccctct
    45361 gcctgggaaa ccacgagttc gattacgggc tggatgtcct ggagagcttc accgcccggg
    45421 cccgcttccc ggtcgtttgc gccaatgccc gcgacgattc gggcgtcttg ccgcacctgc
    45481 ggtcctcggt cgttctcacc ggccggaaag ggacgaagat cgtcctcttc ggcctgattc
    45541 agattgaagc cgggaacggc cttccctcga ccttgcccga acgggttgcg ggcctgcggt
    45601 tcgaggaacc gttcctcaag gcctcggaat taaaggatct gcgaggagac gccccgctgt
    45661 tcatcgggct gacccacctc ggcctgggcc aggaccggaa gctggccggg caaatgcccg
    45721 gcctggacgc catcatcggc ggacactccc ataccagggt ggatccggcc gcgaccgtta
    45781 acggcgtgct ggtcgcccaa gcggggagcg acaaccggtt cctgggccgg gtggacctgg
    45841 tccttcggga cgggcgggtg gcggagaaaa aaggctgcct gatcgacctc gaccgggaaa
    45901 agaaagagga ccgcgaaatc aagcgcctga tcgaggaatt caacgagaat ccggccttgg
    45961 cccgagtcct cgcctcggcc cccttcgaca tcacgggcaa ggaggccttg ggcagcctga
    46021 tgaccgacgc ctggcgccgc gagctcggcc tggacatcgc cttccaaaac aacggcggca
    46081 tccggctccg ccgcctgagc cggaaaatca cggtcaaaga cgtctacgcc cttgatcctt
    46141 tcgaaaacca ggtcgtggtc atgatcatga cgaccgacga gatccgcaac ctgatccgga
    46201 tctcgttcga aaagggggag ggcatcgacc tccaggtttc gggtctcgtc tacacggtgc
    46261 gcatcgacgc cgagcggcgc gttcaggacg tccagctcat cggccaggac ggccggcccc
    46321 tccaagaaga ccggacatac aaggtcggtt tttccagcta catccccagc gcttatgatt
    46381 tcgcccaccg cgatcccggc aagtcgctcg ggatcaccac ggccgacgcc ctgatcaact
    46441 atctcgaaaa gcgtccccgt ctctccggct accagaacgt ccaaagagcc cgcatccggc
    46501 attgagccgc cgaatccggg accggctccc tctctcggga gaccgttcgg atctagaacg
    46561 aagggcggcg ttcgcttgat catggagaac gccgggaaaa gagcttgatg aaaggaaaga
    46621 ggacggccgt ccgcccggag gaaaggccgc cgaccaacag aacgcggcgt ttgagacgga
    46681 cggagggtcc cggcgagacc tctcccggcc caggcttcgg agcgaccgcc ggaacgaaaa
    46741 gaaaagcgcc gccgatcatt gggaacattt caatgacttg accgccgccc ccgttcctcg
    46801 agtaaactag aagaatccga atccgccttc ggagcggatc gattcattac gtccgcaagg
    46861 agccgccgcg atgttggatc attatttcaa gcacgccgac gaacgccggg ccctgggcat
    46921 tccgcccctg ccgctgaatc cggaggatac ggccgaagtc tgccgtctgc tcgaggcccc
    46981 gccggccggc gaggccgacc ggcttctctc ccttctccgg gaccgcgttt cgccgggcgt
    47041 cgatccctcg gccggagtca aggcggcctg gctggccgac gtcgcccgcg gccggaagac
    47101 ctcgcccgtc gttccgcccg aacaagccgt ttttctcctg gggacgatga tcggcggcta
    47161 caatgtcggt ccgctcgtcg aatttctcgg agttcccgcc ctggcccaaa ccgcggccga
    47221 agccttgaag gggacgattc tcgtttacgc ggcgttcgat cgagtcgccg ccctggccgc
    47281 gacgaatacg gcggccgccg gcgtcttgaa atcctgggcc gaaggcgaat ggtttctccg
    47341 ccgccccgcc ttccccaagt ccctgaccct gacggtctac aaggtggacg gggaaatcaa
    47401 caccgacgat ttttccccgg ccaagcaggc cgccacgaga ccggacatcc cgctccacgc
    47461 cctggccatg ggcgggacgc ggtttccgga cggcgcggcc gccatcgccc gattccgggc
    47521 tgaaaacaag cgggtggcct tcgtcggcga cgtcgtcggc acgggctcgt cgcgcaagtc
    47581 cgcctgcaac tcggtcatgt ggcatatcgg ggaggacatt cccttcgttc ccaacaagcg
    47641 ccgcggcggc gtcgtcctcg gaggcttgat cgccccgatc ttcttcaaca ccaccgagga
    47701 ttccggcggc ctgccgatcc agaacgtcga cgtcacgagg atgaacaccg gcgagtcgat
    47761 cgtcctcgat cttgaaaagg ggacgatcat gtcggccgcc ggcgcccttc tctcgacctt
    47821 cgagctcaag ccggcgacga tcaaggacga gttccgggcg ggcggccgtc ttaacttgat
    47881 catcggccgg gccttgaccc agaaagcgcg ggcggccctt cgcctccccg aggcgccgtt
    47941 ctttatcccg gtccgcaatc ccgaggcgaa aaagggacag ccgttctctc aagcccagaa
    48001 gatcgtcggc cgggcttgcg gcgtggacgg catgctcccg ggttcggcgt gcgagccgaa
    48061 aatgacgacc gtcggctccc aggacacgac cggccccatg accgccgacg agctgaagga
    48121 gctcgcctgc ctggaattcc aaaccgatct tttcatgcag tcgttctgcc acacggcggc
    48181 ttatccgaag ccggccgacg tcaagatgca ccggttcctg cccggcttca tcaccgagcg
    48241 gaaaggcgtt tcgctgcggc cgggcgacgg cgtgatccat tcctggctca accgtcttct
    48301 gctgccggac acgatgggaa cgggcggcga ttcccacacc cgcttcccga tcggcttgag
    48361 ctttccggcc ggttcggggc tcgtggcgtt cgccggagcc ctcggcttca tgccccttga
    48421 catgcccgag tccgtcctgg tccgcttcaa ggggaagctc aatcccggct tgacgctgcg
    48481 ggacgcggtc aacgccattc cgtacttcgc catccggcgg gggctgctca ccgtcgccaa
    48541 gaaaggaaag gtcaacgtct tcaacggccg gatcctggag atggaagggc tgccggatct
    48601 gaccgtcgac caggcttatg agctgacgga cgcctcggcc gaacggtcgg cggccggggc
    48661 ggtcatcgcc ttgtcccggg accaagtggc ccggaacctg cggagcaaca tcgccctgat
    48721 gaccaagatg atcgaacaag gctacggaca cgccgaaacc ctccggcggc ggatcgccgc
    48781 ggtcgaggcc tggctggccg agccggcgct gatcgagcgg gatgagaagg ccgtcgacgg
    48841 cggagcgtac gcggccgtcc tcgagattga cttggccgat atccccgagc ctctcctggc
    48901 ttgtcccaac gatcccgacg acgtcaagct cctctcgaag gtcgccggcg acaagatcga
    48961 cgaggtcttc atcggctcct gcatgacgaa catcggccat ttccgggcgg ccggacggat
    49021 cttcgacaag gccggctgcc cgccggccaa gatctggctg acgccgccga cgaaaatgga
    49081 cgagacccgg ctgaaaatgg aagggtacta cgcgatctat tcccaggtgg gggcccggac
    49141 ggagattccg gggtgttcgc tgtgcatggg caaccaagcc cgggtggccc ccaaggcgac
    49201 gatcctgtcc acctcgaccc gcaatttcga cgaccggatg ggcgacgggg cccgggtcta
    49261 tctgggctcg gccgagctgg cggcggtcgc cgccctcaag ggcgcccttc cgagcccggc
    49321 cgaatatttc gagacaatga aggcgaaagt gttgccggcg gccgaggaat tgtacgcccc
    49381 gctggatttc gccgggacca aggattttac cctcggctac gccggggaca ggtgagcgcc
    49441 gtgattaccc tgaacggaca acgcaccctg atcacgggcg gctcccgcgg catcggccgg
    49501 gcggcggcga tcctgttcgc cgaggccggc agcgacgtcg cgttcaatta tgtccgcgac
    49561 gaaaaagcgg cggccgaggt caaggaagcc gtcgaatccc gaggccggct ctgcctggtc
    49621 cggaaagccg acctggcccg ccgggaagac gccgcggccc tggccggggc ggtcgccgac
    49681 gcctggggag gcgtcgacat cctcgtcaac aatcacggca tctggactta cctggaaatg
    49741 ggcgagggaa acgtcgaggc ctggaagacg acgatggagg tcaacctgga cggcgtttat
    49801 tacctgacgg acgccgtcgt cccgcagatg aaggcccgca gcaagggatg catcatcaac
    49861 gtcggatcga cggccggaat ccggggcgag gcccatcatt cccattacgc cgcgaccaag
    49921 ggagccttga acgccctgac caagtcctgg tcctcggagc tggctccctt cggcatccgg
    49981 gtcaattgcg tcgccccggg ctgggtggac acggacatga gcgccggttc gttcgccgac
    50041 ccggccttcc gggagagcat ccgccagtcg atccccctga aacgcatccc gccggccgag
    50101 gatatcgccg gcccgatcct cttcctggcc tccgacctgg cccggcatat caccggggcg
    50161 atcatcaacg tcaacggcgg agcggtcctg tgcggttgac cccggccgaa aaattatgcc
    50221 ttgacatccc tttcgggctt gtgttattca ggagggcggt ttaaagaatt cccatgggaa
    50281 agaagcatct ctccctgatc gtcattccgc ataccaccaa gtctccccgg acatggtcgt
    50341 tttcgcggag gacggtcaag gccgcgacgg tttgcggcat cggtctggcc gttttgatga
    50401 cggccgtcac ggtcgattac gtccgcgtcc gcctttcccg ccggagctac cactccctgg
    50461 tcgccgaaaa cgcgaagcag aaggaagagc tgcgcgttta tgggacgaag gtcggggagc
    50521 tggagcggaa gctgaacggc ttccgggaat acgccggcaa gctgaatgtc ttcgccggga
    50581 tcaagtcgcc ggaaatgttg acgaatcccg gcgtcggcgg cgggcaatcc gcctttccgc
    50641 cggatgcggc tcaagtgatc cctcctcccg gaaatccgcc gccgtcgatc gcctccgtcc
    50701 aggatctgac gcggacggcc gatgaccttg agaaaaacct tgacgccctg ctttcccatt
    50761 tcgagcagct ccagggccag ttcgccaccc ggccctccat cgccccggtc atcggctacc
    50821 gctccagcgg tttcggctgg cgcctcgatc cgttcggctc gggccggcgt gccttccatt
    50881 ccggcctcga catcgccacg gttcatggaa atccggtggt cgccacggcc gacggcgccg
    50941 ttctccgggt cgcctacgac ggcgccctgg ggaacaatat catcatcaac cacggcaacg
    51001 gaatcacgac gctctacggg cacttgagca aagcgcttat caagccgggc gcccgggtca
    51061 agagaggcga tacgatcggc ctcgtcggac ggacgggccg ggcgctgggc gaccacgtcc
    51121 attacgaggt ccgccttaac ggcagcgcgg tcaatcccaa tttgtatatc ttggacgaat
    51181 tctgaccgct attcgagcaa atcccccgct tgaatccctc cccccaagac gaaggcatgg
    51241 gcgtccattt ccgcccggct ttccggatgg aggcgggtga tcagataacg cgtccccccg
    51301 ccgcaagcga ccgtaatccc ctccttggac aggcttgtca ccgtccccgg cgggccctga
    51361 tgggcgggcg tggcgacgcc ttcgacgggc cggccggcga ggatgatcaa ccggcttccc
    51421 cgcagccgga cgaaggccgc cggtttcggg cttaaggccc ggacgcgccg gtcgatttcg
    51481 acggcgctcc gcgtccattc gatccggccg tcctccttgc ggatcttagg cgcgagcgtc
    51541 gccagcgaat gatcctggga acgggggatg atttcatcga tgcggtcgag cgttcggaga
    51601 agaagtccgg ccccgagcga ggccaggcgg gcttcgagct ctccggccgt ttccaaggaa
    51661 ccgatcggag tttcctcctc ggccaggatg tcgccctcgt ccattttctc gttcaagctg
    51721 aagatcgtca cgcccgtccg tgtctctccg gccgcgatcg cggccgcgac cggggcggct
    51781 ccccggaagc ggggcaggag ggagaaatgg acattgaccg tcccccgcgg cggaagggcc
    51841 acgatcgtca tggggatgat ctggccgtag gcgacgacga cttggatatc gggagccgcg
    51901 gcccgaagcc ggtcgaggat ctccggatcc aaacggattt tttccggctg ggcgacgggg
    51961 atcccgcggt cgagggcgaa ccgcttgacc ggaccgggaa gaaggcgcct gcctcgtccg
    52021 acggggcggt cgggttgggc gatgacgaga gggatttcgt ggccggcttc gaggagggcc
    52081 tggaacgagg ggacggccgc ctcgggactt ccgaaaaaga cgacgcgcat gggggcgttc
    52141 agggcttcgc ggccgccgct tttctgaatt tcttccggat caagctgcgc ttcagggggg
    52201 agaggcggtc aacgaacagc ttgccgtcca ggtgatcgat ttcgtggcag aacacccggg
    52261 cgagaagatc ccgggcctcc aaaacccttt cctttccttc aaggtcgagc ccctggaccg
    52321 tgactttctg cggccgggtc actttttcga agacttcggg gacggacagg catccttcct
    52381 cccggatgat ttggccttcc tgggaggtga tttccgggtt gatcatgatg atgagatcgt
    52441 cgggattttc accgatggaa agatcgagga cgatcaggcg gacggaaacg ttgacctggg
    52501 gggcggagag gccgagaccc ggggcggcat ggagggtttc gatcatgtcc cgggccagtg
    52561 tccggacgcc gtcgtcgatg accgtgaccg gagcggactt cgcggccagg gttttatgcc
    52621 cgatggtgat gatgggtctc agggacatgg gccgtttccg caaaacattt taacggaatt
    52681 ttccgttgct ttccaggggg tgaaaaaaag agcgccggcg cggcggcttg ccggcggccg
    52741 ccgggcggct tctcgagagg aaaaaaaaag ggggacggaa caaggttccg tccccctttt
    52801 tccttgcttt acataaccgc cgacggcggc gggggctcat cggcctttcc ggcgttccgg
    52861 atcggcacgt agatcaggta ccaggccatg gccgcgatgg ccaagaggcc gagccagtag
    52921 gacggggcgc tgaagaggcg cggcacgtcg aacttaatgg cgttctgaag gaacaggatt
    52981 ttccagaggg ccttgaacaa gccggtcaat ccctcgccgg cgatcagccc ggcggcgatc
    53041 aggatgccga cgttctccgc ccgggccttc tgggcgggat tgagccggcg cttggccacg
    53101 gccttgtcgg ccagtccctt gatcatgccg ccgacgaaga tggcgaaggt cgtttccagc
    53161 gggaggtaca tgccgacgga aaccagcatc ggagatttga cttggagaag aatcatggcg
    53221 aagcccatca gcattccggc gatgaccagg ggccaggcca tttctccgcc gacgatcccc
    53281 ttggccaggg cggccatcaa gcccgcttgg ggggcggcca gctcgcggct gccgaacgtg
    53341 taggcgccgt ggagcagcat catcggaaaa tacataacgg ccgcggccaa gaagattccg
    53401 atgacgtttc cgacctccat tttccatggc gttccgccga ggatgtgccc gaccttgagg
    53461 tcctggagca tttcgccggc cacggccgag gacacgcaaa cgaccgcggc cacgcccagg
    53521 acggcggcca cgccgctggc cccggccgcc ccgacgacga ccatgaggag ggcggcgatg
    53581 atcaaggtcg aaagagtcag gccggagatg ggattgttgg aggagccgat cgtcccgacg
    53641 aggttccccg agacggcggc gaagaagaag ccggccacgc ccatgacgac cgcggcgatc
    53701 agggccggga aaaacgcccc ggcgaaaacg cggtagacga gaatcatgca gatgaacgtc
    53761 agggccaggc cgcccaggac gatcttgatg ttgaggtctt tttcgagacg ggagacgacg
    53821 tgagtctggc cggtcgcctt cttgacgtcg gagaacgacc gtttgacgcc ggcggacagg
    53881 ttcttccgca tcttgaagag cgtgtagccg gccccgacga gcatccctcc gacggcgatg
    53941 ggccggatga tgaacttcca tacgttggtg accaggccgc cccaatcctc ggcgggaacg
    54001 acggccccgg ccccgtagat catgtcgatc agctgggggc cgaggaagaa catgagcacc
    54061 ggggcgaaca ggccccaggc caggaggccg ccggcgaagt tgagggaggc cagccgcggg
    54121 ccgatgatat agcccacgcc gaggtaggcc gggaaaatgc ccgggcccga gacggagacc
    54181 atgccgccgg cggtcacctt ggtcgaggct tcctccgttc cgagacgaac gaagctctgg
    54241 ccgatcttgc cgatcttgaa gaggaaatcc ttgctggcgg cgtagatgcc gaacatgtca
    54301 agggcataga agaaggaccc ggcgcccatc gcccggaaga ggtgcttggc gctgctgctg
    54361 ccgtgctggc cggccttgtg gatctcggcc gcggccacgg actcgggaaa gccgagctcg
    54421 ggatcggtga ccatgacccg gcggaggaaa gtgacgaaca ggattccgag aaggccgccg
    54481 acgaagagca gcgcggtcgt tttaatataa gcgtcgaagg tgtcgaactt cagccacacg
    54541 ccggcgatca agaaagccgg aatcgtgaaa atggccccgg cggcgaccga ttctccgatg
    54601 gaaccgacgg tccgggcgaa gttctcctca agaatggaac ccttcatcag gcgcaggacg
    54661 gccatgccga tgacggccgc ggggtaggtg gcggcgatcg tcattccggc ccgcaggccg
    54721 aggtaggcat tggccgctcc caggacgacg gtcatcacga gcccgatcaa aagggcccgg
    54781 agggtgaatt ccttcatatc ggttttttct ggaacgaacg gttgatggct catgcggcct
    54841 ccttggaacg gaagccccat ttatatcggg aatcatgttt tcagtcaagg gcggacctct
    54901 tcctcccgtt tccgggcgtc cgccggcggc ggatccgcgt tccattgcgc cccgcggcga
    54961 attgtgttta aatagcgtct ctcaaaagaa ttaggattca cgcaaggaag agttcggcat
    55021 gagtctttat cgctggttca agaacaggct tttctgcgac ttagccatcg acttgggaac
    55081 ggccaacacc ctcgtctaca tgaagggcaa ggggatcatc atccaggaac cgtccatcgt
    55141 cgttctcaac aagcagaccg gcaaggtcga ggccgtcggc cagcgggcca aggagatgct
    55201 cgggaaaacc ccggccaaca tcctggccat ccggccgatg cgagacgggg tgatcgccga
    55261 cttcgagatc gccgagaaga tgctggactt cttcatcaag gaagcgacca acaaccgcgg
    55321 attccttctc cggccgcgca tcgtgatcgg cattcccacc gggatcaccc aagtcgagcg
    55381 ccgggcggtc aaggacgtgg ccatgcgggc caaggcgacc gaggtcttcc ttgtcgagca
    55441 gccgatgtcg gccgccgtcg gggccgagct gccgatttcc gagccgacgg ggaacatggt
    55501 cgtcgacatc ggcggcggga ccacggatat cgccgtcatt tccatggacg gcatcgtctt
    55561 taatcattcg atccggatcg ccggcaacga gatggacgag gcgatccttc agtatctcaa
    55621 gaagaaatac aacctcctga tcggggagcg gtcggccgag caagtcaaga tccaaatcgg
    55681 atcggcctac cccctcgacc agcccctgac catggagatc aagggccgcg acctccgcga
    55741 gggcatcccg aagacgatcg tcatcgatga ccaggagatc cgcgaagccc tcgaggaggt
    55801 cgtgggggcg atcatcaacg ccgttcggat cgccctggaa aaaacgcctc cggagttgtc
    55861 ggccgacatc atcgaccgcg gggtcatcct gaccggcggc ggagctcttc tcaagaacct
    55921 ggacaagcgc atccgggaag agacgcagct gcccgtgttc atcaccgacg atccgctgac
    55981 ggccgtcgtc ctgggagcgg gaaagatgct ggacgatatc gacctgctcc gcaagatcgc
    56041 cattccgtaa aacggcggcc cggccatgcc gcgatttctg aaggagcgca aaaacggact
    56101 tgtgctggcg ggcctgatgt tcgctcaagc ggtcctgctt tccttccaga tccccctcgg
    56161 cgaggaggcc agctatatcg agcgggccgc gttcgccgtc ttcgccccga tccagcacgg
    56221 gattcggagc gcgctccggg gcggcgggga tttcttggga cggtatcttt acctgcgccg
    56281 cgtggaggaa aaaaaccgga ggtaccggga cgaattgttc cggctccggc aggagaacgc
    56341 cctcctccga aacagcctcg aacggctgga ggaccgggcg gccgcggacg cgtttctcaa
    56401 ccggctggac cgggcgttcg tcctggccga cgtcatcggc gtcgatgccc tccagccctt
    56461 caaatcgatc gtcatcaacc gggggacacg ccatggagtg cggctccaga tgccggtcgt
    56521 cgacgcccgg ggacgtctgg tcggacggat catcgccccg atcgattccg gcgaagcgac
    56581 cgtccagctc atcaccgaca acctcagcgc ggtcagcgtc cgcggcctgg agcaccccgt
    56641 ctcggggatc ttgagcgggg acccggcctc cggccgggct tggatgattt atgttccggc
    56701 ctccgacgaa tccttggtcg aaaacgaagc cctggcgacg accgggttcg accgcgtttt
    56761 tccgccggga cttccggccg gaacgatcgt ctccatcggg acggacggat cgcttttcaa
    56821 gaaaatcgcc gtccagccgg ccttcgattt ccgcgacctg gagatcgtcg ctgtcttgac
    56881 cgggccgtcg gggggaaggg aatgagaaac tggatcgacg gcgcgatcgc ggcggcggcc
    56941 gcctttctcg ccttttccct gctccggcct ttgtccgcct ccgccgacct ggtcgtggac
    57001 gtgttctccg tggccgtcct cgtcttcggg acgcacaagg gcgaggcgcc gggggctgtt
    57061 ttcggcgccg cctgcggctt gatcgtcgac gcgttttccc tcggcgtctt cggcctcgcc
    57121 ggtttgagcc tgacgctcgg cggtttcctg gccggatacg tctcccgcaa aatcaacatc
    57181 cagccgtttt tccggatgtt cgcctttttc gccgtcctgg ccgccgggac gttcgccggc
    57241 tgggccgggc tttcggccct gatcggccgg agtcccctcc cgtggtccgg ggggctcctc
    57301 ctcctgcgcc cggcggtatc ggcctcggcg gccgcgatga tcaacctcgg aatccgccga
    57361 tggaaagccc gccgtgagcg ataagggcat ttacgaggat ttttccctgg tccgccgccg
    57421 ggcccggcgc attttcgtga tcgtcgccgt cttgtgcggc ctggccctgg ccgtctactg
    57481 gaaaatccag attctcgatt ataaaaagta cgaatccctg gccgtggcca accggacccg
    57541 ggaaatcatc ctgccggcgc cccgggcgat tctgaccgac cggaccgggg acatcatcct
    57601 ggccgacaac cgggcggctt tcaaggcttc cttcatccgg gagaacaccc gggacgaggc
    57661 ggcctccatc gcccgcgtcg cgtccctcct ggaattggat ccctccgtcg tcgcctcccg
    57721 catcgaaaaa tacaagtctt ggccggcctt ccggcccatc gtcgtcaagg accgattgac
    57781 gttcgaagaa gtggcccgga tcgaggcgcg gaaaaacgac ctgccggaat tgatcgtcga
    57841 gaccgagccg agacgattat acccgcgaca gtcgttctgc tcccatgtcc tcgggtacct
    57901 gcaggaagcc gacgccaacg acctgaagga acgggcccga gagctgcggc ccggcgacat
    57961 ggtcggaaaa atgggaatcg aacaaggata tgaccgtcgc atcgccggcc gggagggacg
    58021 ggtcatcgac gtcgtcgaca gcctcggccg caagcaaagc gagctggagc gcgtcgaccc
    58081 cgtgcctgga aagaagctgg ccctgacgat cgattacgat ctccaagcca aggccgagga
    58141 gctcctggcc ggccgggagg gggcgatcgt cgtcctggat ccctcgaacg gagagatcct
    58201 ggctctggcc agcttcccga cgttcgatcc gaacaaattc atcacccgtt tcacgccgga
    58261 ggaatggcag tcgttcgtca acaatcccga caatcccctt ctcaaccgcg ccttgcaagg
    58321 gcagtattcg cccgggtcgt tgttcaagcc ggtcatgggg atcgcggctc tcgattcccg
    58381 aaccatcggc ctggagacga cgttttattg cggcgggggc atcgagatct acggagccct
    58441 ccgccgctgc tggaatgagg ccggtcacgg cgccctttcc ctgcgcgacg cgatccggta
    58501 ttcttgcaac gtcttcttct atcagctcgg gcgccggatc ccgatcgata ccatcgcccg
    58561 gtatgccgag atgatggggc tggggaggaa aaccggcgtc gacatccccg gcgaaaagga
    58621 agggctggtg ccctcgaccc ggtggaaaaa aaccacgcgc aacgaggagt ggtatcccgg
    58681 agagaccatt tccgtggcga tcggtcaagg gccgctccag gtgacgccgc tgcagatggc
    58741 cgtcttgacc gcccggctgg ccaaccgggg gcggggcatt cggcctcatc tcgttctcgg
    58801 cgcttcggag gagccgccgg gggattccgc tttgaacgtc cggccggagg ttttcgaaca
    58861 ggtgatcgag gggatgtggc ggtcggtcaa cgagggcgga acgggccaag gagcccgcgt
    58921 cgagggcttc gccgcctgcg gaaagaccgg ctcgacccaa acgatcagcc gggagacggc
    58981 cgaacgcatc ggaaccgggg ccaaagccgc gaaaacgcac tcttggttca ccgggttcgc
    59041 gccgcgcgct cagccccggc ttgtcgtcac cgttctcgtc gaatacgggg gcgggggagg
    59101 ggcgacggcc gcgccgatcg ccgggaaatt gttttctctt tataaatcaa accatgatcg
    59161 accgcgttca actcaaggaa attgactggg tgctcacggc cttgatggtc gcgaacactc
    59221 tcgccgggat ccttctgatc tacagcgcct cggccgacag cggcggcgga tacgcctgga
    59281 agcagcttct ttggatggcc gccggattga tcgtcttctt tctgacgatc tccatcgatt
    59341 acaaggcctg gatggccttt gctccttata tctatgcctt gtttctcctc tttatggccg
    59401 gcctgttcct tttcgggcgg acggtctccg gctcccgcag ctggatccgg atcgccttca
    59461 tgggagggca gccgtcggag ctggccaaag tcgccgtctt gttgatgctg gcccgggtct
    59521 tctcggaata ccgggcgccc tacgcctcgc gaggcgtgat cgccctgagc gcgggattgg
    59581 tcggagtccc catgggactg attctcctgc agccggattt cggaacggcc atgtgtttcc
    59641 tgccgattct tttcggcgcc ctggtcctgg ccgggctcac gaagaaaacc gtggccgcga
    59701 tgctcatcgg ggcggtggtg ctcggggcgg ccggctggaa tttctttctc aaggattacc
    59761 agaaaaagcg gatcgagatg atgatcaatc cggcccagga cccgcggggg gccggttatc
    59821 acgtgctcca gtcccagatc gccatcggct cgggcgggct gaccgggaag ggatatttaa
    59881 aaggcccgca gagccggctc cggtttcttc ccgcccgcca cacggatttc atcttcgccg
    59941 tcttggccga ggatttcggc tttgccggcg tttttgcggt cttggccgcc tatttcgcca
    60001 tgctggcccg gatgttctct tccgtcggac tgtcccgcga ccgggccggc atttacatcg
    60061 cgtttttagc cgccggtttg atttccttcc aattcctggt caatgtcttc atgatcgtgg
    60121 gatggctccc gatcaccggg attcccattc cgcttctcag ctacggaggg tcgtcgctcc
    60181 tgtcgacgta tttcgccctg gggctggtcg tcaacgtcaa gatgcggcgg ttcgcgaatg
    60241 tttgacccgg agcgcctctt cccccggatc gaaaaacccg gccgctacac cggcggggaa
    60301 tggaactcca tccgcaagga tcccgcccgg acgagcgtcg ccgtcgcctt ggcctttccc
    60361 gatgtttatg aaatcgggat gtcgtacccg gggcagaaaa tcctttacga tattctcaac
    60421 gcccggcccg atcttcgggc cgagcgtgtc ttcgccccgt ggccggacat ggaggacgcg
    60481 ctccgggcgt ccgggattcc gctgttttcc ctggaaaacc ggacgccgct cgacgccttc
    60541 gacgtcgtcg gattctccct cctgtatgaa ctgaactatt ccaatgtcct gacgatgctc
    60601 gacctcggcc ggatcccgct tttggcggcc gaccggccgg acgatgcccc gctggtcgtc
    60661 ggcggcgggc cggccgtctt caatccggag ccggcggccg cgtttttcga cgcctttctc
    60721 gccggcgacg gggaggaagc gtttccggaa atcatcgatc tgtggatccg cctgaaagcc
    60781 cggggcgagc ggcgggccgg gatcctccgg gccctggccg ggattcgagg cgtttacgtt
    60841 ccgtccttgt acgagacccg gccggccgga actccgccgc tcctcgtcgt ccgcccggcg
    60901 tccggagacg cgccgtttcc ggttgagaaa cgcgtcctcc catcgtttga ccgggcgccc
    60961 tttccctccg ggatcgtcgt tccaaacgtc caatcggtct tcgaccggat cgccgtcgag
    61021 gtcgcccgcg gatgtcccca gaaatgccgc ttctgccagg cgacctcgct gtacgccccc
    61081 ttccggctga gggaagcgga gtcggtggtc gaaacggccc tggacggtgt cgtccgaacc
    61141 ggctacgagg acgcctcgct gttcgccctc tcggtgagcg attatcccta tctttccgag
    61201 accgtccggg cgctcatgtc cgaattggag ccgcggcggg cctcgctttc gctctcggcc
    61261 ctccggccgc ggggcctttc ggccggcatc gccggcgaaa tcacgaaggt ccggaaaacg
    61321 ggcttcaccc tggtggccga ggccgggacg gaccgcctgc gccgggtcat caacaaggac
    61381 gtcagcgatc aggatcttat ggaagccgcg gccaacgcgt tccgccgagg ctggcggctc
    61441 ctgaaaatct acgtgatgat cggcctgccg acggaaacgc gggaggacgt ggaggcgatc
    61501 gccgggttgg ccggccggct gaccctttta ggccgggagt tgacgggcgg atcgccgcag
    61561 atcaacttga gcgtatcctc gttcattccc aagccccata cgccgttcca gtgggcggcc
    61621 atggacggcc cggaaacgct ccaggagaag cagcggctcc tgaaagacct cctcttcaag
    61681 cggagaaatg tcaaggtcaa gacgcacccg gtggagaatt cgctgctgga ggccgtgttt
    61741 tcccgcggag accggcggct cggcggcgtt cttctgcggg cctggcgggc cggggcgcgg
    61801 ttcgacagct ggggcgaccg gttccggttc gacgtgtggc ggcgggcgtt cgaggaggag
    61861 ggagtcgatc cggcggcgta tgtttcctcc ctggatcccg aggccgccct tccctgggac
    61921 cacatggctt ccggcgtggc caaagaattc ttccggcggg aattcttgaa ggccctggcc
    61981 gccgagccca ctccctcctg cctggacgcg tcctgcggcg cctgccgcgg ctgcgccttc
    62041 atcggggcga tcggcaaaaa gccgccggct ccgccgcgat tcacccctcc ggcggccccg
    62101 ccgcctgagc gggcggacgg ggggggagag gaggtcgtcc ggtacgaggc cgtctatatt
    62161 aaggaaggca ggctccggtt catcggacac aacgacctgg tcaatgtcat ccagagaacg
    62221 ttccggcggg ccgggattcc ggcggcctat tcggagggat ttcatcccat gatgcggatg
    62281 gccttcccgc ctccgctggc cctggggatg gaaggccggg acgagcgcct cgaattcaaa
    62341 acgcgggcgg tcctggcccc ggccgtctgc gcggacgcct tgaattcctc ggctcccgac
    62401 gggcttcggt ttcgagggct ccggccgctg gagcgggacg cgccgcccct ttcccggcgc
    62461 cttcgcgggc tggtttacag cgtggacttg ggacatctgg ccgtcgggga cgttttggcc
    62521 ggcccgggac cggacggcgg gacgtccgaa ttcgtccggc ggaaaatcga ggccgccggg
    62581 cttccggccg gcggcccgga gatccgcctg gatccggacc gcaaccgcct ggtcatgacg
    62641 tttcgttttg atccggccaa agccccccgc ccgcaggatg tcctgtcggc cgtcttcggg
    62701 ctgacgaatc cggcttccgt cctcgtccgc gacgcggttc ttgtcgacta agccggcccc
    62761 gccccgtccg gcaaattgac agtcccggac gaatccggta taaaaggaag cgatttttca
    62821 cgccagaaaa agatcacggc agacgcccca aaggagatgt tcatgatgga tttcacttta
    62881 tccgaggagc agcaagccct ccaagaaatg gcccgggagt tcgcccaaaa agagctgcgt
    62941 ccggtggccg ccaaatacga ccaaagccat gagtttgcct atgatgtcat ggaaaaagcg
    63001 ttccaggccg gtttcctgac gggaaacatc cccgaagcct acggcggagg cggcctttcg
    63061 aatctcgacc tggccgtcgt ttccgaggag ctagccgccg gctgcgccgg ccttttcacc
    63121 agcatgatgg cctgttccct ggccaccgcc cccctcattc tcttcgggac cgaggagcaa
    63181 aagaggaaat tcctggagcc gctgtgcagg aaaatgagct tggccgcgtt ctgcctgacc
    63241 gagcgcgagg ccggttcgga cgccggttcc gtccagaccc gggccgtccg ggacggcgac
    63301 gtttacgttc tcaacggcgc gaaatgcttc atcacaaacg gcggcgtggc gagcctgtat
    63361 accgttttcg ccaacaccgc cccggccaag ggcgcccggg gcgtcaccgc cttcctcgtt
    63421 ccccgcgaca cgccggggat cacggtcggc aaagtcgagg acaagatggg ccagcgggcc
    63481 tccaacacgg ccgagatttt cttcgaggac gtccgcgtcc cggcggccaa catcctcggc
    63541 cgcgagcgcc atgggttcat catcgccatg aaaaccttcg accagacccg ggcctcggtc
    63601 ggggcggcct ccgtcggcgt ggcccgggcg gcgatggaag ccgccgtcgc ctacgccaaa
    63661 acccgccggc agttcggaaa gcccatcgcc ctctaccagg ccatccagtt caagatcgcc
    63721 caaatggcca tcgaaatcca ggccgcccgc catctggtct atcacgccgc ctggctggcc
    63781 gaccggggcc tccccaacgg ctctgaatcg gcgatggcca agactttcgc ctcggacacg
    63841 gcgttcgccg cggccaacga ggcgatccag atccacggcg gatacggcta catgagggat
    63901 tacccggtcg agaagtatct ccgggacgcc aagctctttc agatctacga gggaacgaac
    63961 gaaattcagc ggatggtcat cgcccacgag gtgatcgggc cggtcaagga ataaggcccg
    64021 gccggcgggc cgccgcccgc ccggttaaaa atccggggat aaagaaaaag gacgctcgcg
    64081 ggagcgtcct ttttcttttc agccgtccat cgcggacggc gcaaagagag ggattattcg
    64141 tcgatcatct cgggcacgag gccgagcgtg tcgactccgg cgcccttgca gacgtcgatc
    64201 aggtcgacga ccttgctgaa cggcagcttg acgtcggccc gcaggaagat cgtcttgtcg
    64261 gaacgggtgg aatacaggcg gcggagctcg tcgccggcat tctgaagacc gggataattt
    64321 tcattgttga tgttgaccgt cagatccttg ttcagggtgc agacgatcag gccggccggc
    64381 tggccgcccg agccttcgct ggccacttcg ggcaagcgga cgtcgatgcc cttttgaacc
    64441 atgggagtga tgaccatgaa gatgatcagg agcaccagga gaacgtcgca cagggggacg
    64501 acattcggtt cagctttcgc catatcgatt cctcctcgaa aagaccaatc tcaaggcgct
    64561 cattttaatc caaacgccgg ggggaagtca acggatttga aaaattcctc ccggagtcct
    64621 atagtaaccg cctttggagg ccgtcatgaa cgttttggcc gtcctgatcg ccgccgccgc
    64681 cgcctttgtt ctggccggcc gcttctattc ccgcttcatc gcccgccgcc tgggcgagga
    64741 tccctcccgg ccgaccccgg ccgtcgcttt gcgggacgac ctcgacttcg tcccgacgaa
    64801 agtccccgtc gttttcgccc atcatttttc cgcgatcgcc ggcgccggcc cgatcctcgg
    64861 cccgaccatg gccgttcttt acggtttcgt tccggcttgg ctgtgggttc tcctgggcgg
    64921 gattttcatc ggggcggtcc acgatttcac gaccttgttc gtcagtcttc gggaggacgg
    64981 ccggtcggtg gccgaaatcg cccgccgaac gctcggcgag cggggctttc acctgttcat
    65041 cgccttcact ttgatcatga tcgtcctcgt gacgtcgtcg tttctgagcg cgacctcgat
    65101 ctccctgacc tcgctctggc ccctggaaaa gatcggcgtc cgggaagggg agaccttcct
    65161 gagaaccgtc gtcgtggacg gcgtgccgat gggccggatc gggggcatcg cctcgatgtc
    65221 ggttatcgtc atcaccctgt gctcgccgct tctgggattc ctgattcacc ggaagggcgt
    65281 gaagatgacc gtcgcttatc cgatggctgc ggcaatcggc gtgacgtccg tcttcttggg
    65341 catcgctttt cccgtaaccg tctccgcctc cgcctggatg atcatcatct cggtttacgt
    65401 cctgttcgcc gcgggcctgc cggtctggct gatcctccag ccgcgcgatt tcatcaatgt
    65461 ccagatcctt tacgccggca tcgccttgat ggccgtctcc ctggtccgga cgggcttggc
    65521 cggcgtccgg gtgacgatgc ccggcttcaa tctggccgag ggcgtccggg ccttgggatc
    65581 gatctggccg atgatgttct gcatgatcgc ctgcggggcg atttcaggat ttcacgccct
    65641 ggtcgccggc gggacgacga gcaagcagct ttcgagggaa tcgcacgccc ggatcgtcgg
    65701 gtacaacggc atgctcctgg aatcgctcct ggccgtctgc gttcttctgg cgatcggggc
    65761 gttcttgagc ttcggcgatt atcgctcgat cgtttggccg acggagccgg ggatcaaatc
    65821 caatcccatt ctcggattct ccctggccgc gggacatctc ttcaaccggg gcttggggat
    65881 tcccacggcc ttggggaccg tcttcggaat cctgctggtc gagggattcg tcgtcacgac
    65941 gcttgacgcg gccgtccgcc tgaaccgcta tctattcgag gagctttggg aaatcctttt
    66001 ccggaaggtg ccgggctttc tccggaaaca ttggatcaac gccgggattt ccgtccttct
    66061 catgtggtgc ctggcggcct cgaacacctt caccctcctc tggccggtct ttggaacggc
    66121 caaccagctt ctggcggcca tgagcctgct ggccgtctcg gcttggctga tgctgaggag
    66181 gaggagggcg tggtttacgg aaatcccggc cgtcttcatg ctggccacga cgatcgcctc
    66241 cctggcgatc ctgttccgtc agtatctgcg gacgaagaat tatgtcctgg ccgcggccga
    66301 cgcggccttg ttcgtcctgg cggccggagt ggtggtcttg acggtccggt tgttgcggaa
    66361 agaaaggcgg cggacggcgg cggaaaaggc ctgatccaga gccgacccgg gctcagcgca
    66421 gcgtttcgta ggccttttta ttggcgttgg tttggaggac gagcagaatc gcgtcctttc
    66481 ccgcggccgt ggcgtaggcc gtgtgaatgt cgatcacggc gttgcccagg agggcgccga
    66541 cctcggcccc ggccccgatc cggtcgctga tgacgaccgt cagaaccgat tgggctttca
    66601 ccttgtagcc cagagtccgc aaggcggcct cggctttctt attgtccgag gtcagcagcc
    66661 ggaaccgccc tccctcggga tgggacgtca cgcacatcgc ccgaagattg acgccggcgt
    66721 tggccagcgt cccaagaacc cggcccagaa ttcccggctc gttcggggtg atgacttcga
    66781 gctccgactc cactttgacg tccttcacat cgtcctcctc gagggggtca tcataaacca
    66841 gaacgaggcg cgaaggcaag aaatccccgt tttttcaggt gaagcatgaa aaaatactct
    66901 cttgacatta ggccgtcccg gtgttaagaa ttaaatacgt tccatgtacg caaaaaaaat
    66961 ccctttgatc gtccttggaa ggagggcttt atcgtgaaat ccaagaagct gacggtcttt
    67021 ttgattgttc tcctcgcttt cggcctggtc acgtcttacg ccgaaaccaa gaaatggcgg
    67081 cgaaccggct ccaatacctt tgtccggatc aagggcaagg tcccgacggc cgaggtcatg
    67141 aaaacgctgg ccgataaata cgccggcgac atcaagatcg gcatggatca ggttggacag
    67201 ggcgatcttt acgagcccgt cctccaagcc ttgaaggacg ggattttcac tgaagcctcc
    67261 cttccggccg gggaaaaatt cgcctggatg ttcttccgct tgggcggaaa ggtcaaggtt
    67321 tggaccgatg tcgaatgggc cgggaaaaaa cctctcgacg tcttcctttt tgacgtgaaa
    67381 accccgacca agctgtacac gtacgccatg ccccgtccct gcggcaacct ggccttgtac
    67441 aaagcggtcg acgtgattcc gccggctccg ccggcgacct gcctgctcgt ggtcaacccg
    67501 atgaaggcga atctcaacga gcccgtcgtc atcgacatgt gcgggaccca gaacgcggcc
    67561 tcgatgacgg tcgagatctt cgacgcccag ggcgtcaaga tcgccgaccg gaatctgacc
    67621 ccggccgact gcaagtggca gacctccttc gacaaggccg gctcgtatgt cttcaaaggc
    67681 gcggccgtca acgccgacgg cgtcgcctcg tcgaatccct gcgaagtcac ggcctatatc
    67741 aacgccccgc cgacctgcaa gctctggacg tcctgcctcc cctgcgagga ttatgtcggc
    67801 aagccgatca ccttcgacgc caacggctcg accgatcccg acggccagct ggtgaaggcg
    67861 gtcttcgaga tcaccgatga aacgggcaac gtcatcgact cttacacggc caacgccaag
    67921 ccgttcacct ggcagaaggt cttcaccaag gccggcaaat acggcgtcaa cgtctccgtg
    67981 ttcgacgaca tgggcgccgc ctcctccacg gccgatccct gccaccttac cttcgacgtc
    68041 acccagaaga gattcttctg ggtggccgaa atcggcggcc tcctggcccg cggaacctac
    68101 acgggattct tcttcggccg cgtcggcatg ctctggggcg tggttcccga tctgatggac
    68161 atcgtcctga ccatgggccc ggccatcccc tcccagggcg atccttggaa agtcttcttc
    68221 atgggcaacg tcacggccaa cctgcatctc ggcccgtccg tttacgtggg cgcgggattg
    68281 ggatacacga cgaaggagca ggaaacgcgg aagagcggca tcgacctggt cggccagttc
    68341 ggcgtcaaca tcttcaacca ttacacatcg gccggctcga tcttcgccga ggcccggatt
    68401 cccgtcctga cctccgatcg cccggtcgac gatcactaca agcttctgct cggtttccgc
    68461 tacatcttct gagccgcgcg ggtgagattt cccagccgcc ccgcctgaac ggcggggcgg
    68521 cttttttttc atcatgaaaa tcgcgctgat cggggcggac ggacagttgg gcaccgacct
    68581 gaggacggcc ttgaaaggcc acgaggtcgt tcccttgttt tggccggagt tcgacgtcac
    68641 ccgggcggag gaaacggccg ggaagctttc cctcatcggt cccgacgcca tcgtcaacac
    68701 cgccgccttc caccgggtgg atgaatgcga ggaccggccc gaggcggcct tcgccgtcaa
    68761 cgcgatcgcc gtccgggact tggcccggac agcccgggag ctggacgccg ttctcgtcca
    68821 tttcagcacg gattatgtct ttgacgggga gaagaggacg ccgtacgtcg aacaggacct
    68881 tcccgggccg ctcagcgtgt acgcggcgtc gaagctggcc ggagagcatt tcgtccgggc
    68941 gtacgcgggg cgggccttcg tcatccggac ctgcgggctt tacgggaagg ccggctgccg
    69001 ggaaaaggga cggcatttcg tggccgcgat gctccaggcg gccgagtccg gacggaagga
    69061 aatccgggtc gtcgacgacc aggtcgttac tccgacgacg acggccgagc tggccgcgcg
    69121 gatcgccgag ctcctccgga ccgacgccta cggcctttat cacatgaccg ccgagggatc
    69181 gtgttcctgg gcggatttcg cccgggcggt tttcgagctg tccggccggg atccgtcgat
    69241 catcgtttcc gtggattcgg cggccctggg ggccaaggcc cgccggccgg cgtattccgt
    69301 tcttgaaaac gcccggatga aagaaaccgg gctgactcct ttttccttct ggcgggacgc
    69361 cctggccgcc tatctcaagg acgcttcccc cccctcgccg gcccggtagg gcctttctcc
    69421 gggagtcgac cgcggccgaa atcccgtcca tcccggcttt tcagcgacgc ttgtttcggc
    69481 ggtcttagaa cgatcgccgt cggcgggaag gatcgagaga cggggggacg gaatcatccc
    69541 cgggacaggg gaccgagggg acggacggat cggcttattc ggagagcttg ccgagcttcg
    69601 acagctcttc gttatagtac ttgcggtaga ggatctccca ctcccggctg ccttcctcga
    69661 tcggcttttt ctgggattgg atcttgtcgg tcgctttctt gtcgagctgg tcgtagaggc
    69721 gcatctcctc ctcgatcgcc cggacgatcg acaagcgcac ctcgttcggc tcgtcaaggt
    69781 actcgaccgt gtcgtttttc gagagaactt ccagaatcct ggtgctcaga taattgattt
    69841 tttcccggga caggcggctg ctcataggac gatgttccgt tcccgggcca gcttggtttt
    69901 aatcatccgg aacatggttt gatattccaa gttctcccgg cggatttttt ccatgtgttt
    69961 tgtgagaatt tcccggactt ccacgtcgag gcggtcctct ttctggaatt cctcggtgat
    70021 ttgaacggtg atctcctcga ggacacgttc cttgttttcg gccagaattt tgccttcttt
    70081 cagcaaattg cgcaggatca tcccggcaag atgatcgatc tggttcttgt taagtctcat
    70141 ttccagaata cgatacaaca aaagtccggc ttaaatcaag acgaggaaag gcgccgcctc
    70201 gttttccttg atccgggcca gggcgatcag gcggttttcc gggtcgaaaa ggcggaggac
    70261 ggccccgggg gccggagccg gctcgggcgg gacggcgacg gccgatccga aaaggccgat
    70321 cgcccgtccg ttcctgacca aggcccgccc cgcggccgtc accgaaaccg ccggaaggtc
    70381 ctccagaagg ccttccggcg gaatcaaaaa cgcctcaaaa cgtccggcgg cggcggccgc
    70441 ctcgatttcc tccggggttt tggcttcatc cagcctgtat ttgcccatgg ccgtccggac
    70501 gaggcggtgg agatgggcgc cgcagcccag gtccgcgccc aggtcgtggg ccagggaacg
    70561 gacataggtt ccggccgagc aggaaacctc gaaatcgatg tcaggcgggg cgtacgaccg
    70621 aagctcaaac cggtcgacgc ggacggccgc cgggcggcgc tcgacctcgg cgccgccgcg
    70681 ggcgtagacg tacaggggct tgccggccag cttcttggcc gaaaacggcg gagccaactg
    70741 aatgagatcg ccccgaaagc gggccatggc ccggaggacg tcctcctccg cgggcaggga
    70801 atcgaccgcc gggcccaggg ccgttccggt ccggtcgtag gtatcggttg cttggccgag
    70861 acggattacg ccttcgtagg ttttgccgat cgttccgaaa aacgggaaca ggcgggtcgc
    70921 ctttccgatc ccggctaaaa gaagtccctc ggcgaatgga tcgagcgtgc cgaaatggcc
    70981 gattcttttc tccccgagaa ttttccggag acgaacgacg caatcgtggg acgtggggcc
    71041 ggagggtttg cggaggagga ggagcccgtc catgccggcg gtcagcggga gcccgaggag
    71101 gcgggggcgt ccaggagatt ttcgaccgag gccatcacct cggccgaaat gatcggaaac
    71161 gggccgtaca ccgtgaagcc ggcggcgtgg gcgtggccgc cgcccccgaa gtgttcggcg
    71221 atcccggccg cgtgggcctc gcccttggac cggatgctga cccggaagac gtcgggctcc
    71281 atttccttga aaaagaggac gacccggacg cccatgatcg atcgggccag ggtggtgatg
    71341 tcttccgtgt cgacctgccg gaggttgagc tcgatcaaat ccttccggaa catcccgatc
    71401 tgggcgatcg ttccggcggc gttgagccgg agagtcgaga gaacccggcc caggaggatg
    71461 attttttccg gccggttgtt atggaagagg gcttcgctcg tttcgatcgg gcgggctccg
    71521 gccaggacca acttgtggca ggcggcgaag gcccgggcgg tggtgttgga aaactggaac
    71581 gaccccgtgt cggaaacgat ggcgcaataa aggtggttgg cgatcgacgg cgtcagggcg
    71641 aggccggcgg tttcgcataa gtcgagaacg agctcggcga cggccggcgc ctcggcgtcg
    71701 acccagttga tgtcggcgta atggctgttg gaatagtggt ggtcgatgtt gattttgaaa
    71761 tagccgtcca
//
LOCUS       DAOOXH010000003        66572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_362_length_66572_cov_8.314867, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000003 DAOOXH010000000
VERSION     DAOOXH010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 66572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 66572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66572
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_362_length_66572_cov_8.314867"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..867)
                     /locus_tag="PKV64_00770"
     CDS             complement(97..867)
                     /locus_tag="PKV64_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005574067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HPN15638.1"
                     /translation="METATRTAWFRFYEELNDFLPAERRQADFPYSFRGRPSVKDAVE
                     AVGVPHTEVDLILVNGRSVDFHYHLEPGDRVSVYPVFESLDITPVLRLRPEPLRRPSF
                     ILDVHLGKLARRLRCLGFDTVYRNDFTDEQLIGEAERESRIILTRDRNLLKHGRVLRG
                     SWVRSGRLEEQVREIVERFDLARQFRPFTRCLPCNGPVVPVPKSEVEDRLPPRTKAEF
                     HEFTRCRVCGRVYWKGSHYDRLAAVVNHLGGPGRPGAA"
     gene            complement(966..3281)
                     /locus_tag="PKV64_00775"
     CDS             complement(966..3281)
                     /locus_tag="PKV64_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15639.1"
                     /translation="MRRLKTLIPVLVMMWTAASAAVQAAPSTDPSLRLQWAEAFASLK
                     AQTPFPELTWRFIGPDICSGRCTDVAVPRGTTNVIYVGAATGGVWKTEDGGTTWKPIT
                     DELPTPSVGDLAVAGSDPDIVWLGTGEANHFRASIAGLGVYKSVDAGRTWAHMGLAAT
                     QSIGRILIHPENPDVVYVAATGHEWTPNPERGVYKTADGGKTWEKVLFLKDTVGAIDL
                     VMDPKNPDVLIASMVNRIRRRWSDPVPGGEDGLFKTVDGGKTWKELTKGLPDTALTGR
                     IGIDLCASKPDVVYAYVDNHNLGREPESGERDAYGRLKTGRKIIGAEVYRSDDQGESW
                     RKVSVSDALMETFGGTYGWVFGQIRVDPVDENTVYLMGLGLFVSRDGGKSWTEIARDV
                     HGDHHGLWINPDDPAFLVNVNDGGVALSRDGGKTWRAFIKEIPAVQFYNVALDMAEPF
                     SAHGSVQDHGTIRGMGLAPAKNGAADPVRWEYAAGGEGTQIAVDPADPNVVYSSSFYG
                     RLMRSVRQDGEWKSKNIYPRPAPGEPEHRGQWLAATILSPHDSKTVYHGFQYVYRSRD
                     EGATWERISPDLTAFDPDKQGRLPYAIPYASITTLDESPLKAGVLYAGTDDGHLWVTR
                     DGGAEWTKITAGLPENTHVWTIEASRHDPATVYCALVGRHEDDFAPYVFASTDYGNSW
                     TPITANMPGGPVNVIREDPKKKGVLYAGTDQGVFVSRDGVSAWQYLGKGLPVCPVWDI
                     RIHPRDNMMVIATNGRGMWVLDDLSRIQKST"
     gene            complement(3285..5684)
                     /locus_tag="PKV64_00780"
     CDS             complement(3285..5684)
                     /locus_tag="PKV64_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15640.1"
                     /translation="MSLRRFPRILTLSFSVFILALLLFPQQTKKPVRIANTDPALRLQ
                     AYDQHVKMKGESKFKDLTWQFLGPKNVSGRCIDVAVATPKGKTYTMYVAAASGGLWKT
                     DNEGTTWTPVFEQGPSTAIGDVALAPSNQDIVWIGTGEANIFRSSQSGVGVYRSTDAG
                     RTWTHRGLAGTGTIARIVIHPKNPDIVYVAAGGTEWTTNPDRGVYKTVDGGQTWDKVL
                     FVNDETGAYDLVMDPRDSDTLYATTWQRTRKKWNDPRNTAVSAGSGIWKTTDGGKTWT
                     PVNAGLPEARYRGRIGIDLCLSKPDILYAFVDNYEKAREPSEEEKINGYGLPSAGIIK
                     GATVYRSDDAGKTWVQTSGLTPEQKKFMERHSNTYGWVFGQVRVDPNDENTVYTMGLS
                     LNVSRDGGKTFSRIRTNGGDHHALWIDPSNSLYLLNGFDQGLNVSYDGGKTWRYFQAE
                     LPLCQFFNINYDMAEPFRVYGSMQDHGSYRAKVDVSGGRDKIAPQDFEDAPGGEGSNH
                     AVDPTNPNIVYSAGFYGSITRSDYSRPGRYPGYPASKNILPVPYQDEPRLRGQWLAPF
                     LVSPHNPSILYHGMQYVFRSFDRGDTWEKISPDLTSAVPSEMGDIPYHTLFALSESPL
                     KDGLIYAGTDDGNVWVTKDGGRKWTAINAGLPYQKWVSRLVASQHKMSVVYMTQNGKR
                     DDDVAPYVWKSEDFGKTWVDISKGIPLGPVNVIREDPVDPKILYAGTDQGVYATTDGG
                     ATWNVLGGNLPSAYVHDLIIHPRDNMIVIATHGRGMWVLDANPVNGKDKRRAWAWWEE
                     E"
     gene            5933..6472
                     /gene="pgsA"
                     /locus_tag="PKV64_00785"
     CDS             5933..6472
                     /gene="pgsA"
                     /locus_tag="PKV64_00785"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPN15641.1"
                     /translation="MNLPNALTSLRIFLIPFFMFFLLVDFKGNEWAAFGVFLFATFTD
                     TVDGLLARRTNRITTLGQLLDPIADKLLMTAAFICLVGVGRVPAWMAVVIIGREVAVT
                     GFRAIAASKGVVIPASWPGKLKMQLETVTIALLVLGEGILGKFWVVGKIGLWLTMAIA
                     LYSAAEYFVKHGRDIVLEK"
     gene            complement(6491..7396)
                     /gene="fabD"
                     /locus_tag="PKV64_00790"
     CDS             complement(6491..7396)
                     /gene="fabD"
                     /locus_tag="PKV64_00790"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPN15642.1"
                     /translation="MARQVFLFPGQGSQSVGMGKSFYDRFEDARRLFDRADDVLGFSL
                     SKICFEGPEETLRLTQNTQPALLLVSTAAFRLWGRTPYAAAGHSLGEYSAHVAAGTIR
                     FEDALTLVHKRGTYMQDAVPVGVGAMAALLGADYEAVRAACGRAEGGQVQIANWNSPE
                     QIVISGHKAAVEDVVRNVRSVKSVMLPVSAPFHSGLMKDAEDRLAADLDAVEFHDPAF
                     PVITNVDARMIRTGAEARDALKRQVSRPVLWTKSMELLAGEEIDAFIEFGPGKVLCGL
                     IKRIGKGWAPPFTVRSIEDAEALDK"
     gene            complement(7428..8096)
                     /locus_tag="PKV64_00795"
     CDS             complement(7428..8096)
                     /locus_tag="PKV64_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15643.1"
                     /translation="MRLRTIGTLVLALLPVLVSAAGGTAGGRERIVDGIVAVVNSRII
                     TLIDVQVVEAFGIVEGSPALPGGGRDLRDVLEKLISQKVVLDLVRGRSTADPARVEAE
                     IERITARLGEEETGRRLARFGIGKDDLRPYVEEKLGVETVIAERFSRSVTVSLDEIET
                     RYRERYLPAEQAAGRTPRPFLETVDVLEGEIRVEKTAAQSNLWVQSLREQAEIEIRPE
                     LLIQ"
     gene            complement(8101..10842)
                     /locus_tag="PKV64_00800"
     CDS             complement(8101..10842)
                     /locus_tag="PKV64_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HPN15644.1"
                     /translation="MPRSGKGFRAVLALLAVLAAAESRPASAVASGARISAVLVRVEG
                     EEIRPDLRSLVPIADGDPFLPVRIDEAVKRINETGLFSDIEVRAEGEEDVRLTFLLTR
                     KLFTRRISFSGEVPVSRKSLLSGLYALRPETEFSEPRLRRAEEELRENLRREGFFDAR
                     ATGRAVPDPARRFVDLEFTVEAGRRFSVRNIEFRGGLGEVQAKAVKSLATRPGRPYRP
                     AVLESDLDVIRAAYRAAGYPRADVAVQTRFFRAADASVGLVLGVDPRERIRITIRGAE
                     VPESLVRPLWEERIFEEWGLKQAEAAILTSLRRKGHVFAVVRSSIEREAGDLHFIHQV
                     DPGPKIRISSVVFEGLTAFSEAEIRRRIGLAPRLPLFGSVSGDQLFAAPEEVETLYER
                     EGYPDAGVTLQFRERGDGYQAVFVIDEGPVRTIRSVVFRNISLVPENDLRRDLECRES
                     GPYEPGRVRRDAERLESRYLNGGIRGTAVAGRAEALDDGRFDVFFDIQEGTPVRIDKI
                     VVSGNAVTRRDVIDRALLLREGDPARADLILESKRRLEKLGVFVEVKIEEILSTESGR
                     ETLVVSLREGARNYAGLGVGLETKNEPMTFEIWNNVIRPRFSAEFIRGNMLGRAAQLS
                     LIGQFSLKEKRAVASWESPAFFGLPFQSSVNAWLEREERVSYGFDRRGFSLSGFRSIG
                     PDWTSLTTLRFARTTLYFLEVEESEIDRQHYPYSATSLSESLIWDARDDSFNPTRGLF
                     FSAVLEWAYPLFKVESDYLKSFVKFQYFQPIGGRILLLGTFRSGLGMGRIPIHERFFA
                     GGSGTFRGRRFDDLGPKDAKSGKPVGGKALILFNIDAQIAPFSSLPGLSLAVFYDRGN
                     VFASRKDVGFDALEDALGFGFRYKTPLGPLRVDLGWNLDPSAGGRGPRLYVTIGNVF"
     gene            complement(10817..14230)
                     /locus_tag="PKV64_00805"
     CDS             complement(10817..14230)
                     /locus_tag="PKV64_00805"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HPN15645.1"
                     /translation="MKIGRKLKSLIALLAVAGGLAGGGVVVRKVILSQIRKQIDPVMR
                     YDRLRLTFLPPTAVLENVHSLKSDPFFSIDRIVLQIPFRSILRNNRAFNVFIRGLTAR
                     IYEKPGRDAKPPSLTLRLPFFVENGVLEEGEISFWGRGASLITRNVRAYFRQSKDAFV
                     VRAEAASHSINVPAIAHPLEGSSDVYFEARGDELILRRLTVHHPDLLVKARGTLIHPQ
                     NPVIDIQATVRGPVALVADAFNLPFDWTGGVAGTARFVRGETGQSRLTADFESRDMTF
                     TGETVGRVSGDLALSAGVGKINLEISKPWAPVESLDLTFGGGRLAGRVRGVHVDPLIK
                     EIGIPWPVLSPVWGEFTLLPEALEVQGEFRDEPADERNGRFPFRGSFRLHWDHAKKVV
                     FSSERIESNFAVMRVDGRLDIGREAQVEISGDVTDIPRGRAFTSLALRMPFTFPEIRG
                     RGRADVKILGAWHVPEVKIDFALTPGSFGRFEAAEGAGIVEIARGETTGIVRVRDPEL
                     AGLLRFTVRKGGWACRVEDGEGTLEKIFPSLGLDLPFAGRVSGGLEFHDDGAGIEAEG
                     DFASPRAVLYGQTVENVKGRFGWSTADYRLTLSGLTGTYYGGAVSGSMAMGFHSEDFA
                     IDAAFRGIDLSGPAPAVRGRLDFDLKGGGNFARDEASGLLKITGFGVGPVENIEITGT
                     AAAGMKNKAFGARFTGEVNPGRNKIEASFRYPETDGGFFVGASGMIYNFDLFLPWSGA
                     KGEIRCLAEVRGGGASPARWNGILDLKGAVFPFPKFAHALTDYSGLIRVDNNVATIRS
                     LRGKLGGGDVFGSGEVRFGPGGISLIDLRAEGRDMTLSVFERTRGLADGSFRFLKNAR
                     EYVLAGDVDVKRLLWRREVNEKFIFSASPYPEARKGPGLFDDLALDIRIHADDGAMLE
                     NALGKMEGRLDLTLSGTVASPIVLGDLEAVKGEVYFQDRSFRVLKARLSFFNPTTIEP
                     YIDFRGETYLKDYRVTLSVAGLLQSLRPEFTSSPPLPAEDVLALLSMGESFKRTYSYD
                     ATSQVGTGSLMSFQLADSAARGAERLFRLDRFRIDPFVLGSSTEMTARLTVGKKLSRN
                     LMLLYSTNLTSQREEIVRLEWEFLENFSLVGMRDERGRLSLDAKVRKRF"
     gene            complement(14317..15090)
                     /locus_tag="PKV64_00810"
     CDS             complement(14317..15090)
                     /locus_tag="PKV64_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPN15646.1"
                     /translation="MKIARARFKDRVLHGAVDDDRLIPIEGSIFADFVVGGEPIPLAG
                     VQLLAPVGPSKIVAVGLNYRDHAAERGKPLPEEPLLFLKPSTAVIGPDGAIVYPRMSR
                     RVDYEGELAVVMKKRASRLSALDPVESFILGYACFNDVTARDLQDKEKQFTRAKSFDT
                     FAAIGPWIAAGLDPSALEIRTRLNGKLRQSSNTRNLIFSVPDLVRFISNVMTLLPGDV
                     IATGTPAGIGPMVPGDEVEVEIEGIGTLRNRVLRIHGEA"
     gene            complement(15087..16199)
                     /locus_tag="PKV64_00815"
     CDS             complement(15087..16199)
                     /locus_tag="PKV64_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPN15647.1"
                     /translation="MGKIRPATVLGLAILAAAACGRKGPILEPLTRIPQPVASLAAGQ
                     RGATLRLRWTAPAAYIDGRALPPDARTEIWLMRNAAAEAALPMAWEDEDEFTAKAVLL
                     SVLDSYGRPLQAGTAAVPPPAAPPLKEFSWEREMTPEDGAAARLVFGVRVAAGRRGAS
                     KFAYAGWLPLQAPAPPGGLKARVFEDRIELGWNVPKDAAGTPPRQGFNVYRSSAGGPE
                     VRLNETPVPAPPFADRTFRFGVSYAYRVSAVAGEKEPFIESRDSEALEVTPADSFPPA
                     RPKGLISVTAVGLITLSWDPGPETDLAGYRVWRKGPRETGFSLMTPAPISENTFTDDQ
                     VEAGGRYQYAVTALDRTGNESGRSEVLTETGKGAGS"
     gene            complement(16199..17602)
                     /gene="hslU"
                     /locus_tag="PKV64_00820"
     CDS             complement(16199..17602)
                     /gene="hslU"
                     /locus_tag="PKV64_00820"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HPN15648.1"
                     /translation="MTIHLPKSLAGPLDKLREKDREAELTPREITAELDKYIIGQNAA
                     KKAVAVALRNRYRRRRVPADLADEITPKNILMIGPTGVGKTEISRRLAKLTVSPFLKI
                     EASKFTEVGYVGRDVESIVRDLVRMAVDMIRQEKTLEVRDKASAGAEEKILDLLLPPV
                     RRRNGVVSDEDHQRYLESREKLRRQLRDGLLEDRIVEIEVRDRSTPPLEIFSNQGVEE
                     IGLQIQEILPGFMGGRTKKRKVKVSEAREYFLDEEERRLVDMDLVARLAVERVERSGI
                     VFLDELDKIAGRESGRGPDVSREGVQRDLLPIVEGTTVHTRYGLVRTDHILFIGAGAF
                     NVAKPSDLIPELQGRFPIRVELTSLNKADFVRILTEPENALIKQYKALLETEGVEITI
                     TADAIDEIAQTAEDVNREAEDIGARRLHTIMEKVLEEISFQAPDIAAKKITVDRTYVR
                     NALQDIVKNADLRRFIL"
     gene            complement(17603..18130)
                     /gene="hslV"
                     /locus_tag="PKV64_00825"
     CDS             complement(17603..18130)
                     /gene="hslV"
                     /locus_tag="PKV64_00825"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HPN15649.1"
                     /translation="MIRSTTIVCVRRDGRTAIAGDGQVSLGETILKSSASKVRRLHKG
                     QVAAGFAGSTSDAFALFARFETKLEEYKGNLYRAAIELAKDWRLDKALRQLQALMIVA
                     DKDSALMISGTGDVLESDDGVLAIGSGGPYALAAARVLLKHTTLSARDVAEEALRTAA
                     SICVYTNDTITIEEI"
     gene            18348..19292
                     /locus_tag="PKV64_00830"
     CDS             18348..19292
                     /locus_tag="PKV64_00830"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPN15650.1"
                     /translation="MARKIGRREFIRSGAALGAAVVVGGKLFGKWTGNPPSGESGPSV
                     FVVKGTDSAAMTAKAVELAGGLGAYVPKGAKVALLPNVQSRHPGTFTKPEILRAVLRM
                     CRQAEAAEIACLSWLGPTNWDGTGLARVIEEEGAALRLIPREDENFKSVPIPGAVALP
                     EARIMNALFEYDLWINMPITKDHAGNKFTGTMKNLMGLNAPSSNRAQFHKPNWTTDPA
                     DIAHLEYCIVDLNFAVRPAFNIVDATEIIRTNGPMGPGELIKPGRIVAGVDRVAVDAY
                     CATLLGFQGAEITAIKHASERKLGEIDLRKAGVLEVEI"
     gene            19303..20238
                     /locus_tag="PKV64_00835"
     CDS             19303..20238
                     /locus_tag="PKV64_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15651.1"
                     /translation="MKKAAAGPLAAVLLAGFFQGGGTASIPFPVPDSDPAALIPAAGE
                     VPGWTPRGEAQRFDGEDLYVYIDGGAEIFNEYGFRRVIAQDFENPGGAGIALEIYEMT
                     DPAAAFGIYSFQRSGQGQAAGLGTEGEIEDYYLRFWKGPYLAVVTGYDPHGRDPGRLM
                     EGVIAVARAVDARIQESGGRPAFVEALPPDWMEPGLKYLRGVLGLNNLVRLFPRDVLR
                     FREAAAVPIEGGWLFVCGYRDAAEAESRLAGVREAMSAREGGYGEFQAFPDGRLEAAD
                     SRGNRIVVRAAGDKIGLVVSPRAAAVAEALLERLR"
     gene            20316..22007
                     /locus_tag="PKV64_00840"
     CDS             20316..22007
                     /locus_tag="PKV64_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HPN15652.1"
                     /translation="MRVRISAAIAGFAVIGSLGFSAAGPAGSADPLRAVLSPLNPAFV
                     RALERGDVRHPSPQDITPLQGLLPFVDPTLPSAYDLRDEYRVTGVRGQFPVVTCTIFG
                     LFASLESTLKPYETCDFSERHLDGGSEESDALEANVGAMARWSDPVLEDDDPWEGGTP
                     YDPARPAVKHVQKVVFLPPRADALDNDRIKRAVMDRGGVAVEMRFSRSLLNDTYDSYY
                     TTVETPDVHAVAVIGWDDLFAKEKFTPRAPGDGAFICKNSLGGNFGAAGYFYVSYYDA
                     FFGRFSLPAVVLAEPASGLIENYQYDPVGCTARLGFSSETAWFANQFVAVSNDPLAAV
                     SFYSYGEAGEYEVFVYTGAAPGLPQSGTLAAQFAGTLTEAGYHTLRLPEPVPLAVNER
                     FSVVVRLRSQGNNYPIPIEHPVPGFDADFNALPGQSFISSDGQIWSDLVTYEGPTYAR
                     SNVCLKAFAGYAPVYPPAYLRVERLVNDLIFYKEYVDRLSWAVHPSNTEPIAAYRIYR
                     KEKGAPNDSYVFLAETGTTDLSYYVRGLTATSAYAYRVTAVTASGRESDPSEVVD"
     gene            22018..22581
                     /locus_tag="PKV64_00845"
     CDS             22018..22581
                     /locus_tag="PKV64_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPN15653.1"
                     /translation="MKKTALSIVILLAWAGLSPAQTWEAAVAGGYRTLKDSALREIYG
                     NGFVVAPSLSLAMSKTLRVGAEYEFGYSKNAAIGMFEDPSTLKVRGGHLFVQYGERSG
                     RFRPFIKTGVGIFAYQFDVRTPSQPTVNVSETDVSFFIGGGVRAFFTKRLFGTAELKY
                     VALWVGSYDDKVDLGGIRLLLGVGYDF"
     gene            complement(22661..23818)
                     /locus_tag="PKV64_00850"
     CDS             complement(22661..23818)
                     /locus_tag="PKV64_00850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPN15654.1"
                     /translation="MHLPETADIVIAGGGVIGASTAYHLAKGGAKNVVLLEREPLFGT
                     ESTGKCAGGVRAHFDTEINCRMSALSLPAFERFEEETGYSADYRKIGYLFVLNKEQDV
                     AAFQRQMDMHLALGLRTEWLTGDEIRRRLPMMNFEDALGGLFGPDDGLADNGAVVQGY
                     VSAASRLGVRCLTGVEVTGVETAGGRVQAVLTSAGRIPTPLVVNAAGAWAGEIGRMAG
                     LEVPVQPVKRQLFTTTPTPEIPADFTFVIEFGTGLYFHREGPGLLSGMSRADQPPSFD
                     QAVDEEWELTHLETAAFRLPLMEQKGIARRWAGLYEVTPDHHAIIGPVEELKGFYMNA
                     GFSGHGFMHSPAAGRLAAEEILKGRTESLDISCLRMERFRTGKTLDEYNVF"
     gene            24080..27112
                     /locus_tag="PKV64_00855"
     CDS             24080..27112
                     /locus_tag="PKV64_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15655.1"
                     /translation="MPVFSKTFLRLFLCALVPAVLAGTVAAQGPAAGSDRPLFRHLSW
                     RSIGPANMVGRISDFEALDADFTHVLVASASGGVFKSINAGTTWEPIFDRYGSASIGD
                     VAWFQKDPDIIWVGTGEECSRNSVAWGDGIYKSTDGGKTFTRMGLETTHSIGRVLTHP
                     TDRDTVYAAAPGHTWGYSGDRGLFKTVDGGRTWIKLGGGLPNDGKTGAIDLVMDPTNP
                     DVLYVGFWQRLRRPWRFDSGGPNGGIFKSSDGGRTWIKLTRGLPEGDTGRIGLAISRS
                     NPKVLMASVEHGYQPRPTIPGEAGKPPKDNPDYKDMTKLGTGIYRSEDGGESWVFVSR
                     MNNRPFYYSHIYINPVEDQWVYSLATIMNFSSDGGRTWTPMEGLHPDFHAMWLDPTNK
                     NRMYVGQDGGAFLSHDNGKTWIIFDNLSFAQFYAVSADMRDPYYVYGGLQDNGTWGGP
                     SLHREGSILTDFWYNVGGGDGFHVQIDPTDWRILYSESQGGAISRWNVETRESKSIRP
                     NRGNIANYKDYFSDPPEPERKPGAENVPPADPETAWREMARRMRGPFRFNWSTPILLS
                     PHNPRTLYFGGNQLFKSTDRGEKWMIISPDLTANDAEKNKPTGGLTDDTTGAETHGTI
                     ISISESRLRPGLLWVGTDDGNVQISRNDGASWTNVRPNIPGVPAGLWCSRVEASPFDE
                     GTAYAAFDGHRSDDFRVYVFKTTDYGKTWTSISKGIPDGQPVYVIKEDLKKKNLLFLG
                     TEFGVFASLDGGRSWDSFSLNMPTVAVHDLFIHPREHDLIAATHGRGLWIMDDLSALR
                     QAVDLPASTEAAVLEAGRPGTKWLTIARGGYGRGDLYFKGENPPQGAMIHYYFKEKPQ
                     QPVSLEITDITGRRKTVYILDDIQPGVGRLVWDFLFDPAPAAVQSSAAALKTQLEAAR
                     ERTEATAEQKAAIAAALNELDAAGGNHRKIQEVRRKSMSLIGRGMFGRYGGRYPGGPG
                     MANLEAEAGKYAVKLIVGDKTYAGDIDVRLDPLNSEPG"
     gene            complement(27170..27553)
                     /locus_tag="PKV64_00860"
     CDS             complement(27170..27553)
                     /locus_tag="PKV64_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15656.1"
                     /translation="MGKDWLQPGEEEIGSWSVYIGGQGPMDAKVTGKIHITNLNFHFA
                     AGLSLEKNAASLMTNYQHIKAFEKSEEHLTIPFSEIAGGEIVKKGFLLKSLAVKLKAG
                     EELLFHFGAMNPQKALDALNAGLKK"
     gene            27961..29013
                     /locus_tag="PKV64_00865"
     CDS             27961..29013
                     /locus_tag="PKV64_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15657.1"
                     /translation="MSLRKRLILTTAAVLLTGLAGFAQQTFQTAVYFDYSHFITTDGY
                     LTSNTLTNKFHFRRAYFTYENKINDRLKFRFRYDADNTANLTSVDFAKQTVKKDDKLR
                     PFVKHLYLEWSPNFLASKVNAGMIETLAFKLAEDRWGYRSVAKTLLDGYKDITGVDVD
                     ATSADLGLSWKGTLLRQLRFGLGVYNGSHYSHAETDKFKKFSGYLQLVPAAGLSLIGY
                     VDYEKQTGDRKAMTTKVDVLFDMVRNLNISFEWFAYDSDNLLNSNGTHFKPSGWSVFG
                     TYKIKPDKLAVFARYDRYEPNDTKADDEIGLIIAGFDWTPMHGSWKLQPNVWIYNYND
                     ARKGDTIFNLTFFLSF"
     gene            29037..29951
                     /locus_tag="PKV64_00870"
     CDS             29037..29951
                     /locus_tag="PKV64_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HPN15658.1"
                     /translation="MMNEKWIKVLVLTGLVLAVIFTPILLSAQTKMLQVKGSDTMVNL
                     AQILAEEFMAQNPKMPIAVLGGGSGTGITGLINGTCDIANSSRDWKAKELNLAFEKGV
                     KPRLFAVAVDGLSIIINEKNPLTKLTMEQVGAIYRGEVKNWKGVGGPDQKISLYGRQS
                     NSGTYAFLMEHVMGNRPYSTDMKEMNGNAQIIEGVLADASAIGYVGVGYVVDKNTGKA
                     LKGLKILDISQAPNGTAYSPLDKTAVDSGQYPISRPLWMAVNGTPKDVAAAFLRYAVG
                     PEGQKVVEREGFYTVGGRLQEQNQKNLK"
     gene            30091..30984
                     /gene="pstC"
                     /locus_tag="PKV64_00875"
     CDS             30091..30984
                     /gene="pstC"
                     /locus_tag="PKV64_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPN15659.1"
                     /translation="MISRRLRNLKERAIRAFFFLNGILVVVILAGIFVLLLSSAVPAF
                     REIDLKEFLFGALWDPTSPIRASYGILTQIYSTFLVTLGAMILAVPVGLAVAAYLSDV
                     APGRVREIIKPVVEILAGVPSVVIGFLGIVVLGPALSKLTGESHGLNALNGSILLAIM
                     SLPTIISISEDSLAAVPRSYSEGSLALGGSRWQTLLRVKFPAALSGIIAAVMLGMGRA
                     IGETMTVLMATGNARSFPHGLFDSVRTMTSNVAIELGEVPYFTTHYYALFAIGLVLFL
                     LTFLINLAADRVLHKYRERER"
     gene            30981..31853
                     /gene="pstA"
                     /locus_tag="PKV64_00880"
     CDS             30981..31853
                     /gene="pstA"
                     /locus_tag="PKV64_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPN15660.1"
                     /translation="MKISLRLRKAVAQSLGFFGLRVALGLVLLVIVFILADIGTKGSG
                     VISWEFLSRPPRRGMTEGGIFPAIYGTLLVTLITAVLAVPLGMGAAVYLNEYAPKNRL
                     TRLIRLSIRNLAGVPSIVYGLFGVILFVNIFKFGTSILSAGLTLGLLTLPWTITASEE
                     ALKTVPNSFREGALALGATPWQCIRTNVLPYAVPGMLTGTILGLARAAGETAPIMFTG
                     AAFALPILPQSIRDQFMALPYHLYIMATQHHDIATVRPLAYGTALVLIVLVLGLNLGA
                     VLLRYRLRRRHKEY"
     gene            31855..32619
                     /gene="pstB"
                     /locus_tag="PKV64_00885"
     CDS             31855..32619
                     /gene="pstB"
                     /locus_tag="PKV64_00885"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPN15661.1"
                     /translation="MNSRPKIETKNLNFFYGSKRALKNLNIACHKKKVTAIIGPSGCG
                     KSTFIRTLNRMNDVIPGTRATGDILVDGAGILGPKVDVVELRKRIGMVFQKPNPFPKS
                     IFDNVAYGLRINGMGKSREKTAEIVEKSLREAALWDEVKDRLKESAFALSGGQQQRLC
                     IARALAVEPEVILFDEPCSAIDPIATAKIEDLIARLKKTYTVVIVTHNMQQAARVSDY
                     TAFLMLGELIEFDETKTIFTSPANQMTEDYITGRFG"
     gene            32676..33359
                     /locus_tag="PKV64_00890"
     CDS             32676..33359
                     /locus_tag="PKV64_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN15662.1"
                     /translation="MNEKIAVVEDEPDLLRLTAEALGREGFKVAAFPDATSFLRSLDK
                     TRPDLILLDLMLPDADGLDVCRAIRGHDARRSIPIIIMTAKGTETDRVLGLELGADDY
                     VVKPVAFRELAARVRAVLRRGKADAADQKISLGNGEFTMDPERFEARLSGKPVELTTT
                     EFKLLHLLASKPGRVFTRDQILDRLWGNDKIVLDRTVDVHIKNLREKLGPAARFLKNI
                     RGVGYKIDN"
     gene            33624..35480
                     /locus_tag="PKV64_00895"
     CDS             33624..35480
                     /locus_tag="PKV64_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN15663.1"
                     /translation="MKRSVSFAVVFLLALFFGFNPPAAASDSDAVENAQKDERLLRPR
                     HEVTVTASPIWKAVKDCSVSVGILDETDILAARPLNALGALTRLPGIFAHRTGDFGRT
                     DIEIRGIGQRGQRIAVLVDGRPEKMGLFGCAVTHAYPLDNVERIEVVRGPSSVLYGPD
                     AMGGVINVITRRPASGFRTELTTAVGSYKTFSLNLRHGAAFERFDYYFTADRMTSDGH
                     TAHSSYLGTAFTGRAAYDLGSGLTLTLRGKAFDGKKNEPGPLASPSLTSWNHYKRGSA
                     DLSLNGRWEGGEGSIMAYSDFGRHVFSDGWSSRDHYRGAIARGSLALGADHRLSFGLD
                     ARFFGGKSYNAPRGEWEKSDVGVYLHDEILLGGRWILSAGARLDRDSQFGWEGSPQAG
                     LVWHAGENTSLRLSASKAFRAPHINELYLFPASNPGLKPERSWNFELGISQAVARILA
                     FDAAVFTMKGTNLIESSPNPAGPPPFKFRNTGRFAFSGIELGLKADVLPSLMLRGAYS
                     RLDTGGLTKGRPGRKIDLEIRWQAGRFRTFLTAQSVSEYYAGDRKTLPIPSYTLVNGR
                     LEFAVTPSVGLFAELNNLSAAACWIYADLSGKAAGLYEMPGRNAHVGLKVGL"
     gene            35477..36061
                     /locus_tag="PKV64_00900"
     CDS             35477..36061
                     /locus_tag="PKV64_00900"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN15664.1"
                     /translation="MSRPPLGGFFDRCAPSWDNGCPAGLTAAISKIFAAARLHPGSRI
                     LDVGAGTGILYPYFLKAGAGAYTAVDSSPAMVQRFKAKHPEAAVLEADYEKPIVFPEP
                     FDVIMIFNAFPHFRDEEAVFRRSRSYLSAGGRLFICHSMNREALNAHHRDAGGTVAGD
                     VLISDARMAGLYRKAGFTAVRVENTDHFYSEGTK"
     gene            36058..36567
                     /locus_tag="PKV64_00905"
     CDS             36058..36567
                     /locus_tag="PKV64_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15665.1"
                     /translation="MKPELKDVSTAAVFGAAALALPVLFHALGLGSAFLPMFLPLSAA
                     GFLLPYRAAGPLAVIVPAASFAMTGMPPMTIPPTGPIMMLELAFLMAANRFLHRRLKI
                     SVFPAAAAAAVAERLFYLGLLFLAASVLRLPRLAFSLAALVKSLPGTILLLTAVPAVV
                     KLIQKHAEA"
     gene            36567..37175
                     /locus_tag="PKV64_00910"
     CDS             36567..37175
                     /locus_tag="PKV64_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15666.1"
                     /translation="MRAALLLLFQAAALLADPRFLAPATVAAAAWFAGRKESRSRVLL
                     GTGVFGMFFFVLCLLGAVEGFLRLSAPDYGRYALLTLRGYASFLVVVAGTAMFTLAEI
                     LCFLKRIRVPGYILTMVYLMVHDLSVFVRLASETGRAVRSRGAGLRLPQRLKLTIHAA
                     RNFMVFAVLKFRLRHEHIQARGLSLDLPLDEWRARERLPQSG"
     gene            37150..37842
                     /locus_tag="PKV64_00915"
     CDS             37150..37842
                     /locus_tag="PKV64_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN15667.1"
                     /translation="MNACLKADDLSFWRGERAVVSGLSFTAAPGDRIAVLGANGSGKT
                     TLIRTILGFSPYRGTLEVEGREVRDRPKEARKAAAAVFGDADTQLLMPTVYDELAFSL
                     KAAEERAGGPDVRAFAGRFGLVPLLDRHPSQLSSGEKRKVLLAQSLGRRPSILLLDEP
                     TSDLDARGSRRLREMLAQVPQTIILATHHYDLAMALCRKAVVLGGGKAAVYEDIRRLF
                     DDPAALDRWELL"
     gene            complement(38035..40902)
                     /locus_tag="PKV64_00920"
     CDS             complement(38035..40902)
                     /locus_tag="PKV64_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPN15668.1"
                     /translation="MKRSVPAALIILALFAGLGLGAPTAKPPATFTDYGQWETIAAAG
                     AYGGFSPDGRWLVYGLNRSNRNNELRIVKISDKTEKAFAFGSRPAYSSDSKWLAFAVG
                     LSEADAKKLKDEKKTVQDKLGLLNLATGDVTTFDGIESFAFSPDGASLAMKTYPPEAA
                     ADAPNRSGGPDTDGASPGSTLLVRRLATGLDTPFGNVSQYLWEDGPKGRFLALIVSVK
                     GKTGNGVHLFDPDSGVLRVLDSSSSAYAGLSWRKDAPDLAVFRAKTDDRKEGTAQVVL
                     AWTGLGANERIWVYDSAADAAFPAGQRIVDFRPLSWSASGDAVFLGLAPWDDAVAPAE
                     TKAPAGGSAAAEEVSTIEIWHAKDVFVMPWQKIHADAGRRRNSLAALRLSTGKLVPLG
                     LDPVHEEVTPLRSGRSALVAEWSKYAFNRTIGRPAADLYLADIATGARTKIREGIDDA
                     HAQASPGGKYVLFLQDDHYWTVDTATRAVVNLTASLPVSFIDKESDETGPAKPPFGVA
                     GWGKEDGAVLLYDKYDLWRIAPDGSGAARLTNGAGEQIRHRLVRLDLADGGRRRYGFP
                     PPPAEAVDLTAPLYLELFGEWTKKSGYARLRPGGGITRLIWLDRAVDGLAKAEKAEVY
                     GYTIEDYDVSPDIHVGGPDLKGAVKVTATNPFQEKFAWGRSELIEYVTEKGRRLQAAL
                     IYPAGYEPGKQYPMIVYNYELLSQNVHRYAAPSERSYYNASVFSSQGYFVLEPDIVFR
                     ERRPGWSVVECVTAAVKKVVETGLVDPAKVGIVGHSMGGFNTAFVATHTDGIFAAAVA
                     GAPIIDMVSYYGDHHWDTGIAETDHIETGQERMVVPLYEDLQAYIDNSPYFGTHRMTV
                     PLLLEVADEDKIVPWHQGIELYNVARRAGKNVVMLAYMGEGHGLRKEKNQMDYQRRIL
                     AWFGHYLKGEPAEDWITSGQSYLEREAEIKRERAKQTSRSD"
     gene            complement(41134..42555)
                     /locus_tag="PKV64_00925"
     CDS             complement(41134..42555)
                     /locus_tag="PKV64_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15669.1"
                     /translation="MKKTAMFLSVGLLAASLAFAAETTGPAAPEITGRVRLFSSFYAA
                     DNPDGAFFTHKAGQFAFRRAEVRLKLSGAVNDRVSYGVRFDAFSYPGDLLQAGQFPES
                     AFLGSPASSEHFELALYEGWVKVGDFLIEGLDLTAGKQRISWGTADKIGVVDNLNPID
                     FANFLSFDPDYFFERRPQTAVNLEYYPGGSFKIQLVGLLQHQVSPLPFGYSGLVAKFF
                     PMDEYLVTRGWDDTAIKTAWGARVAGRAAGLDLGVSYYRGNLTLPYLRSMDAGLVASG
                     VFYYPRAGILGLDLAGEAGGLGYWAEAAYVMPEKIDGSVSFPIWIGDGPVIYTKTFPL
                     FEEGFWKYVAGADYNFGGGFSVNIQFLHGFFDEVDYNAGAEDTFGFRKGMFFGEPGNY
                     VLGRAEYKFARDTVKLKFGGLFETTSNGSAFVIMPEAEFRVADAFNVVCGAFAAVSGD
                     REKTKFGAFRDDGQIYLGFKLDF"
     gene            complement(42566..43336)
                     /locus_tag="PKV64_00930"
     CDS             complement(42566..43336)
                     /locus_tag="PKV64_00930"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPN15670.1"
                     /translation="MKRTSLILASVLLLIAASAPAAAAEDAAAVLAGVDERTGGTLAP
                     KDMQSNMVMTITQGSSVKVREIRAWTRNNAGKNDDRLMKFVSPADVRDVGFLVLSDDS
                     MYIYLPEFHRTRRIASSNRKDSFMSSDFSYDDLGTGEYARSYEPALQEENAGAWVLDL
                     KRKAGSDKLYSRIVMTVDKRMHMPVKTEMYDEAGRLWKSAEQAYASIDGYWVMTAVKM
                     TDHKKGSSTTLEFKDMKVDKGVGADLFTERSLVRKIQG"
     gene            complement(43333..46224)
                     /locus_tag="PKV64_00935"
     CDS             complement(43333..46224)
                     /locus_tag="PKV64_00935"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPN15671.1"
                     /translation="MAKKWTAFVVRHRVLVLGLCGLLTLAALAGVPRLRFYNKLTDWL
                     PKDDPHLALYHEISDTFSANNIVLVIARPKAGVFTADTLARIRELTDTLKDREEIFSV
                     TSLSNVADIRKTEDGIEVRDFINSVPVDSAGLKALEALARSKDRYVGQVLSEDGEWFG
                     LSVYLSNTVETIHAVEKVLIPETERILGGGMEIYFAGTPSDGHFINLYTRRDLVFLVP
                     IMLAAIVLILYFTLRSRKALLPPVLVVVLANIWLFGLIGWSGRPMTIITPAAPVLLLA
                     LGSAYGLYVVNKIRSDVETGRNIDVKQRRERVIVSTAAVVTPILYAAVTDIIGFLSFR
                     GVKLSLVADFGLFAAIGLFFAAGLAVTLLPALAATIDFGDVRPSKSRFRLSGFLESAA
                     GRIIRRPRLALAAFGILIAFGIAGIFRVEREVGFAGFYAKKSMPYRAMDAANEHFKGA
                     YPDSFFFEADDVRDPARLRLIRRAESYLSGLQSVHQPLGIPDLIEELNDSMNDRPALP
                     ETKAAVENLWLFLEGRKELTQMITPDGRQTIIYAKVSDSTTPFCEETFALAESFFEDE
                     EAMRPVRVDLKTLSPSGAAAVRAAEAAFLAQEIAWAAGESGGPAPGAGPIRDLLVAAL
                     NKPLPADRLGPAVVEKIRDFCFAPSFPFEAAPRELESISRALSGLAAASPDFEIAEEA
                     AAAVFKKHLKPAEFDPDLAADAAASAAYLVRETRESLQEASLREALAALLPAGSPSFE
                     KRAAAILYDLVDGLAVVPAADAPAGSEPVDFIRVDQTGYPVMTTKLADSLLRSQIQSI
                     ILAMIVTLLLMILMRRSVVLGFISILPITFATVMMYGLLGFVRIPLDYATMLTGSISI
                     GVGIDYTIHFLYVVTEEIRAGHALPEAIRLAFIERGRAMLSNTVAVAAGFSVLLLSSF
                     VLLRSFGGIMVLSMLLCFIGAMTLLPAALLVFRPRVLRPAGEIMKGDSI"
     gene            complement(46217..46852)
                     /locus_tag="PKV64_00940"
     CDS             complement(46217..46852)
                     /locus_tag="PKV64_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPN15672.1"
                     /translation="MKKLAFEGILEKRKAQIVTAVLLLVDKVGITGLTIKRIAKEVGF
                     VEGALYKHVDSKMGLFHLILDASFRSIESVDREISSRTSAAPAALRAWFEFVVSFLEQ
                     YPGIHRILFSDALYVEDRPLFKKFKDCMLDLKGRLRKNIETGVASGDFRADADPEIQA
                     INFLGVIYTTFTLWTVFEERARSYMETARPYIEDYLRSLSLPVPAAEVSRG"
     gene            complement(47145..48278)
                     /locus_tag="PKV64_00945"
     CDS             complement(47145..48278)
                     /locus_tag="PKV64_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN15673.1"
                     /translation="MRRLKRILRIAGNDIRIMVKDKVFFFWTLAFPILFILLFGYLYK
                     GGETAPPSAELTVLNQDEGRWGAYFLEKIKSPGIVLKTAGTEPAEYNRLIILPPDFSE
                     KIEARKDQALALKKREDASAAAAARVEIRLYQAIARLLSELVLYGDGDLTKFLDGHAE
                     FRDLVTVKTRYPAKTITVVPGGMDHAIPGITVQFIMMMVLIYGGVTVMEDRKKGVLSR
                     LLFSPLSPGALYQAKLLARWLMGMLQALMLFVVGKIFFRLNLGDVPLSLLVIAVFALA
                     TAALSVFIGSLMGKEDMIIGLSILLANVFAGLGGCWWPIEVVPPAVRTMGMISPAYWA
                     MDALHKLRFFQGDFAAVAPNLGVLLALAVVLSILAGRFFKIRD"
     gene            complement(48282..49526)
                     /locus_tag="PKV64_00950"
     CDS             complement(48282..49526)
                     /locus_tag="PKV64_00950"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN15674.1"
                     /translation="MRRLLLILKNDILRHLKAPVAILLYLIIPIAMTGLIGMIFTPSS
                     KDNTLPPIQVVLVDKDKGLASRLFLGAFDADQMKKMFQVTVADEAEGRRRMEKGKASA
                     MVVIPARFTLDVLEIKPVVLEVVKNPAEQFLPDVVEEFMNTMAVMLSGAIQAFEPEAR
                     GIRLMLDGPIDAFPWESLGPEFGKAQKKVVAAEKYLNPLLIRLQEEKTKAAGAKAFMT
                     RSDIFSAVLPGMAVMFLLFIVQTVMRDMISEREDGKLRRMLTTPLRPGELIGARIAGG
                     WLMGFAILLVMAVFGTLVFKAYWGSPGYFLLLAAVTAFWTASFFALMNAFLKNRNQAG
                     AISAPVILAFSVFGGSMLNTEAMPKAFQAIGLATPNRWFIDGAALVREGRFPAAALLV
                     LAASGLILLFLAVPLLRRRSTV"
     gene            complement(49530..50459)
                     /locus_tag="PKV64_00955"
     CDS             complement(49530..50459)
                     /locus_tag="PKV64_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN15675.1"
                     /translation="MLEIKNLTKSYGDLLALDHFSLTLERGEVLGLLGPNGAGKTTLI
                     SILAGTVRDFTGSVRFLGHDLFSDRNLKNRLGIVPQDMAFYEELNAMDNLLFWGGLYD
                     VPRDALKKRAAEILERVELSGRMKEPVKNFSGGMKRRLNTAIGLLHRPDLLLLDEPTV
                     GIDVQAKVSILDLIRKVGAEGTGVIFTTHQLAEVENTCSRIAIMDCGRILAQGTMEEL
                     IRLVGEKDIVEITGNFPAASFTEALQDLDGNGIELLSAADNLATLAVADTEQIPKTMD
                     RLFRRKLAVTDLKIKTPSLETVFLKLTGRSLRD"
     gene            complement(50904..51320)
                     /locus_tag="PKV64_00960"
     CDS             complement(50904..51320)
                     /locus_tag="PKV64_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15676.1"
                     /translation="MSEEKRKVLEMLSQGKITVDEAEKLLAAIGEPDNEAVSSTGGKR
                     QWKYLRVVVDPGPGSESKDKVNIRIPFKLIRAGLKFAAFIPREYQGQIDEALKEKGMN
                     VDLSKITPEDLEEIVSNLDDMTVEVDGEDKVRVFCE"
     gene            complement(51349..51624)
                     /locus_tag="PKV64_00965"
     CDS             complement(51349..51624)
                     /locus_tag="PKV64_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15677.1"
                     /translation="MLPFWLRLRVCKPGRRGFRLWFPVILIWIILGALLIALFPLVLL
                     AALITWRNGPGRFLLFLYPMLAAVLWNLSGLHIEAKDDENDVLIDFA"
     gene            complement(51636..51998)
                     /locus_tag="PKV64_00970"
     CDS             complement(51636..51998)
                     /locus_tag="PKV64_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HPN15678.1"
                     /translation="MTQDWSELISLTQGVSFTVEKVRLKNRDIAIEGSFELPPLARLR
                     AEDVLFIIAFVRSHGSIKEMEAQFGISYPTVKNRLNAIAAQLPFVDVNPPSAKRDVLD
                     QLDRGEIDVTEALKRIKE"
     gene            52484..54640
                     /locus_tag="PKV64_00975"
     CDS             52484..54640
                     /locus_tag="PKV64_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPN15679.1"
                     /translation="MQRASFKFVLTLLAMILIISAAAVPLPAAVLPSPTPQAEPSAEP
                     TLAGHWEGIIELPGQKLEINIDFVLKEGAWTGDISIPVQKSQDLPLIGIVLDGFKAAF
                     AIQGIPGEPTFKGEISADGKKMTGTFTQGGAKFPFSLELGADPTAKIRAALEGFDQIA
                     EDALKALKVTGAAMAVVKNDKVVYAKGFGYKDVENKIPATPDTIFAIGSSSKAFTVFA
                     LGRLVDEGKLEWEAPVRNYIPWFKLYDKEAGARLSVRDLVTHRSGLPRHDLLWYNNKT
                     ASREDLVRALAYLPPTADLREKWQYNNLMFLTAGYLLETLTGKPWEDAVRSLVFEPLG
                     MKRSNFSVLDSQKDSDFAFPYNFVDKAYEKLPFRDITNVGPAGSINSSVNEMARWVAV
                     HLADGKFEGRDLLSSSTVSDMHNPHMVTGAVSSSPDIAATAYAMGWFTDVYRGHRRVH
                     HGGNIDGFSAQVAFLPDDGLGFVVLTNMNGTSYPELLVRTATDKILGLEAHDWIGDAV
                     KQLEKAEGAIKKAEEKKITRKVPGTKPAHPLADYAGLYVHPGYGSLAVELAAGRLSFT
                     YNSIKTNLDHWHYETFDGVTIEDPTFKDFKLTFRTDVNGRVASVTGAFEPTIDDIVFA
                     KKPDARQYDPAFLKKLTGRYDLMGQTVVIGLRGDRLTVSIAGQPEYQLEPDLGGEFYL
                     KEVKSVTLRFLTDAKGEVTAAELSQPGGVFEAKKLE"
     gene            complement(54880..56241)
                     /locus_tag="PKV64_00980"
     CDS             complement(54880..56241)
                     /locus_tag="PKV64_00980"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPN15680.1"
                     /translation="MSNLTPSLHLKGRPVVTSTVAARMAALKIPAVSIAVTRDSRIEW
                     TRAYGFRDVEEKLPATPETLFQAASISKPVAAAAALHFVDRGVLGLDEDVNAKLSSWK
                     VPENSFTAKQKVTLRRILSHSAGLTVHGFAGYPAGEPLPALRQILDGEPPANSAPIRV
                     NLLPGFRWRYSGGGFTVLQQLLVDVIGRPFPDIMKEIVLDPAGMTESAYAQPLPDRLL
                     SRAAAGYTSDGTPVGGRRHVYPELAAAGLWTTPADLCKFALEIAAARDGRSDKVLSQK
                     TAQAMLTIEKAPSGLGFMLDGMGADVRFSHSGGNAGFLCDLVFFPERGAGMAVMTNSE
                     NGSALIAEIERAIAAEYGLPAFRPVIEKEIVPMDAAALERFAGNYEIDFEGQKTPVTI
                     AVKDDHLAATAADSLREIYPESEMSFFAMDLSLSLLFLKDAGGRITGFIVDGTYPAKR
                     TDK"
     gene            complement(56985..58553)
                     /locus_tag="PKV64_00985"
     CDS             complement(56985..58553)
                     /locus_tag="PKV64_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HPN15681.1"
                     /translation="MADFVTSAGGDASSAPAAGPEPGPDRKPDSLQIGVSRPPLTLDD
                     LPTPEEVFDKPRIGFREIVTVVLGPSMIALGASLGSGEWLLGPLAFGKYGFLGLGWLI
                     VISGILQVLYNVENARYVIATGEVPIVGFSRVPPGFKLWIPATLFIIILGWIWGGWAA
                     AAGQSLFALFSGRAADASRPGEVEAYRLIAVGLMFLSLLIYSVGRKIERTLALVDTFM
                     VILTLGAVVILAVVFAPGALWVEMLRGIVTPAAPPSGIDPVLLGSIIGYTGFGSGMNF
                     LLINYYRDHGYGMGHKVGFFSGLVGGEKKSVRPSGMTFRESPENAALWKRWFRYLKID
                     QWGVFFTGAMIGMFVPGVLVRALAAAPGAAEPTTENIPVYAAVELGRRGGFFFVFVLI
                     IGAMILFKTQTSVLEILIRNVTDSAIAVSPRLRERINGDPRRAYYGMAVLFILVIAVI
                     IHLALPARLLQIAGNMSTLASLIYPVLLIYLNTKLPRPARAGGWSIAVLVLNILFFGY
                     FFLNFAWSMIAGRP"
     gene            complement(58949..59824)
                     /locus_tag="PKV64_00990"
     CDS             complement(58949..59824)
                     /locus_tag="PKV64_00990"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPN15682.1"
                     /translation="MKNRPAAQARPRPRQDPPIIAVCYDFDGTLSPRNMQEYDYIPKL
                     RLSSRKFWREAQERAREQGADEIHSYMRLMLEKATPASGVQITKKAFIDYGRTIPLFP
                     GVKSWFPRMNAFGSRLGASVRHFIISSGIREMILGTPIAGEFERIYASSFMFDQNGVA
                     YWPALALNFTTKTQFLFRINKGVLDPWDDSRINDFIPMAERPVPFSRMIYIGDGTTDI
                     PCMKLVKEQRGYSIAVFPPGPGKKAVAERLRGENRVNFTAPADYRAGRPLDLQVKATL
                     RKIMAEYALLGGEKK"
     gene            complement(59991..60731)
                     /locus_tag="PKV64_00995"
     CDS             complement(59991..60731)
                     /locus_tag="PKV64_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HPN15683.1"
                     /translation="MFTLATVVAFGALTGFSLILGSLIGTSLKISQRAVAAVMAFGSG
                     VLICALTFGLMEEAFKHGGFDAIIIGFLSGGLVFIAGDLLIHRLGGRSHRPKLRVPAA
                     RETNGKAIILGSLLDGIPESVALGVALLDRKGIGFLMLAAVFLSNLPESLSSIDDLRK
                     EGFSGRRITLSWSIVGVCVAAAVVLSFLFLHHLDPNALGIIESFASGAILAMLADGMM
                     PEAYENGGFLTAFMTMSGFLTAFILSKI"
     gene            complement(60817..61905)
                     /locus_tag="PKV64_01000"
     CDS             complement(60817..61905)
                     /locus_tag="PKV64_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPN15684.1"
                     /translation="MAKFEGLKWSSKQMRYGLADKSYELLESDAKIAACSRSTNYAIF
                     LAFEGIRFFCRKNASGGLEVVFLNWDKNLERFRRSMLFNIGKDSAPLVPTADELEDIL
                     ADRFFREPAMRGLFEEMAAMGAQGYLRPFTVDEEQSIGVTFPNQPAIRALASRYDQYL
                     GEPFTGVVIPHLVRAVGLNGTGCLKLGVNYVMSVKAVEAAKTIDPSAAAALFLDDQTH
                     RPVEERHITEWDSSCCLFAFTDGTVVKIPESPLILPSVTIQGICKILTHLGVKVEERH
                     LSYGELLRRVRAKELATVASIGTAGILNRCAKLLLVDGDQKIVATHAPDERHALYAKL
                     AEAKAYYWNIYKGVVPPLDGMKLFKYSI"
     gene            62261..62845
                     /gene="ruvA"
                     /locus_tag="PKV64_01005"
     CDS             62261..62845
                     /gene="ruvA"
                     /locus_tag="PKV64_01005"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPN15685.1"
                     /translation="MIAYLKGVIRQKSPSQIVVDCGGVGYAAAIPLSTFFKMGEEGEP
                     VELFIHTHLTDSALSLFGFRTREEKELFLKLISISGIGPKLAIAVLSGMDTETLAEAV
                     RASDVARIAMVPGIGKKTALRIAMELADKIESKEKVLKARAPKETEDLVSALLNMGFR
                     RKEIEPVVENVVKSGKNGDFEHRLRACLKALSKV"
     gene            62847..63914
                     /gene="ruvB"
                     /locus_tag="PKV64_01010"
     CDS             62847..63914
                     /gene="ruvB"
                     /locus_tag="PKV64_01010"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPN15686.1"
                     /translation="MPPLAPSAVLNPVRTKEDLLFEDSLRPRSFDEFIGQDNIKANLK
                     IYIQAAKKRGEHLDHVLLYGPPGLGKTSLAYLLSRELGVGLKPTAGPVIERAGDLSAI
                     LSNMQSKEVLFIDEIHRLHPSVEEILYSAMEDFSLDIVIGQGPAARSHKLMLKKFTLV
                     GATTRAGKITAPLRGRFGIIHRLDYYKDADIKKIILRAADLLKVKIDDTGAEQIAKRA
                     RGTPRVANRLLRRVRDHVDVKGKGMITAAAADDALDLMEVDSLGLDDVDRKILTTIIE
                     NFGGGPVGISTIAAAIDEEKDTIESIYEPFLMRIGFLERTIRGRKLTRKAYDHFGLPH
                     PGRGRAAGAAGGTAQKKLFRD"
     gene            63949..65079
                     /locus_tag="PKV64_01015"
     CDS             63949..65079
                     /locus_tag="PKV64_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HPN15687.1"
                     /translation="MLVKEYDFPLPPELIAQKPLARRDDSRMMVVRRKEGTFAHAAFR
                     DFPDYFAAGDLLVINDTKVIPAKIWGRRGGSLIEFLLFKQREPGTWEVLCRPARKIRP
                     GDRVDFPGGLSAEVIETGDEGRRVLKFAVPDVLGRLRDIGFAPLPPYIKRPKNDDGGR
                     AEDLNRYQTTYAENEGAIAAPTAGLHFTPDTMERLKARGVEAARVTLQVGLATFQPLR
                     VERVEDHRMLEEIYTVPEEAVAAVERAKAGGRPVTAVGTTVVRTLESAARAGEAGMTG
                     DAAPAAREAGPAAEAGEPGGRNPAAEILIAPGTRGTSLFIYPGFEFKVVDRLLTNFHL
                     PQSTLLMLISAFAGRELIKAAYEEAVRKKYRFFSYGDCMLIL"
     gene            complement(65293..>66572)
                     /locus_tag="PKV64_01020"
     CDS             complement(65293..>66572)
                     /locus_tag="PKV64_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN15688.1"
                     /translation="KPLLGDQRRVPGEGRTAFAAAPLPRVRIGFVGVGGMGSAHVQNL
                     LNIDGVQIKALCDIVPAKVERAQKWVVEAGQPLPSAYTRGDHDFERMCETEDLDLVFT
                     ATPWEWHVPVCLSAMRNGKHAATEVPAAMTLDDCWALVETAEKTGRHCQMMENCCYDR
                     IELMTLNLVRRGVLGEILHAEAGYLHDLREVKFSSEGEGLWRRAWSRKLNGNLYPTHG
                     LGPVAQCLNINRGDALDFIVSMSSPARGLHEYAAANFGPSSPQAAEKYALGDVNSTLI
                     RTKLGKTIILIHDTNLPRPYTRINLVQGTKGVAQKWPDRVYVEGLAAESHAWDDFEKF
                     AAEYEHPLWKAIAAKGEGRGHGGMDYIEDYRLIQSLLKGEPLDQDVYDAAAWSAVVGA
                     SCGSVAAGSRPFEIPDFTRGKWKINPPLGIVTG"
BASE COUNT        12597 a        21056 c        20625 g        12294 t
ORIGIN      
        1 tccggagcgg ggaaaagagg gcctccccgc cccccaagaa gccggacgct tccgcaacga
       61 tcgccagcga tgaaaacggg gccggacgca ggcgcctcaa gccgcgcccg gacggcccgg
      121 cccgccgaga tgattcacga cggccgcaag gcggtcgtaa tgcgaccctt tccaatagac
      181 ccgcccgcag acgcggcaac gggtgaattc gtgaaattcg gctttcgttc tcggaggcag
      241 acggtcctcc acttcggact tcgggacggg aaccaccggg ccgttgcagg gcaggcagcg
      301 ggtgaacgga cggaattgac gggccaggtc gaatcgctcc acgatctccc ggacctgttc
      361 ctcgagacgg ccggagcgca cccaggagcc ccggaggacg cggccgtgct tcaggagatt
      421 cctatcccgg gtcaagatga tgcgcgattc cctctcggct tctccgatca gctgttcatc
      481 cgtgaaatcg ttgcggtaga ccgtgtcgaa tccgaggcat ctcaggcggc gggccagctt
      541 gcccaggtgg acgtccagga tgaacgacgg acggcgcagc ggttccggcc gcagccgaag
      601 gacgggggtg atgtccagcg actcgaaaac cggatagacg gagacccggt ctccgggttc
      661 gaggtgataa tgaaaatcca ccgaccgccc gttgaccagg atcaagtcca cttcggtgtg
      721 cgggacgccg acggcctcaa cggcgtcctt caccgaaggg cggccccgaa acgaatacgg
      781 gaaatcggcc tgccgccgtt ccgccgggag gaaatcgttc aattcctcgt agaaacggaa
      841 ccaagccgtc cgtgtcgccg tctccatcga aacgaatcct tcggcggccg acctcgggcc
      901 gcggcccgct ccggaacaac gcgccgtccg cccggcggcg tctcttattt aaccggctct
      961 cgggatcagg tcgatttctg aatccgggaa aggtcgtcca ggacccacat tccccggccg
     1021 ttggtggcga tgaccatcat gttgtcgcgg ggatggatcc ggatgtccca gaccgggcaa
     1081 acgggcaagc ccttcccgag atattgccag gcgctcaccc cgtcccggct gacgaagacg
     1141 ccctgatccg tgccggcgta gagcacgccc ttttttttcg gatcctcgcg gatgacgttg
     1201 accggtccgc cgggcatgtt cgccgtgatc ggcgtccagg agtttccgta atccgtggag
     1261 gcgaaaacgt agggcgcgaa atcatcctcg tggcgcccga ccagggcgca atagacggtc
     1321 gccggatcgt gccgcgaggc ctcgatcgtc cagacgtggg tgttctccgg caggccggcg
     1381 gtgatcttcg tccattccgc cccgccgtcc cgggtcaccc agagatggcc gtcatcggtc
     1441 ccggcgtaaa gaacgccggc tttcaagggc gattcgtcca gggtcgtgat cgacgcgtaa
     1501 gggatggcgt agggcaggcg gccctgcttg tcgggatcga acgccgtgag gtccgggctg
     1561 attctctccc acgtcgctcc ttcgtcccgc gaccggtaga cgtactggaa accatgatag
     1621 acggttttcg agtcgtgggg cgaaaggatg gtcgcggcca gccactggcc gcggtgctcg
     1681 ggctcgcccg gggccggccg gggatagatg tttttcgact tccattcccc gtcttggcgg
     1741 accgaccgca tgagccggcc gtaaaacgac gaggaataga ccacgttcgg atcggccggg
     1801 tcgacggcga tctgcgttcc ctcgcctccg gccgcgtact cccagcggac cgggtccgcc
     1861 gccccgttct tggccggggc gaggcccatc ccccggatcg tgccgtggtc ctggaccgag
     1921 ccgtgggcgg agaacggctc cgccatgtcc agggcgacgt tatagaactg gacggccggg
     1981 atttccttga tgaacgcccg ccaggtcttt cccccgtccc gggacagggc gactccgccg
     2041 tcgttgacat tgaccaggaa ggccggatcg tcgggattga tccacaggcc gtggtggtcg
     2101 ccatggacgt cccgggcgat ctcggtccag gatttcccgc cgtcccgcga gacgaacagg
     2161 cccaagccca tgaggtagac ggtgttttcg tccaccgggt cgacccggat ctggccgaag
     2221 acccagccgt aggttccgcc gaacgtctcc atcagggcgt cggagacgct gacttttcgc
     2281 cagctctcgc cctggtcgtc ggagcggtag acctcggccc cgatgatttt ccggccggtt
     2341 ttcagacgcc cgtaggcgtc gcgctcgccc gattcgggct cacggccgag gttgtggttg
     2401 tcgacatagg catagacaac gtcgggcttc gaggcgcaga ggtcgatccc gatccgtccc
     2461 gtcagggccg tgtcggggag tcccttcgtc agctccttcc aggtcttgcc gccgtcgacg
     2521 gtcttgaaca ggccgtcctc cccgcccgga acgggatcgc tccagcgccg gcggatgcga
     2581 ttgaccatcg aggcgatcag cacgtcggga ttcttcgggt ccatcaccag atcgatcgcc
     2641 ccgaccgtgt ccttgaggaa aaggactttc tcccaggttt tcccgccgtc ggcggtttta
     2701 taaacccccc gttcgggatt cggcgtccat tcgtggccgg tggccgcgac atagacgacg
     2761 tcgggatttt cgggatggat caggatccgg ccgatggact gagtggcggc cagtcccatg
     2821 tgcgcccagg tccggccggc gtcgacggat ttatagacgc cgaggccggc gatggacgct
     2881 cgaaagtggt tggcctcccc cgtccccagc cagacgatat cgggatcgga gccggccacg
     2941 gccaggtctc ccaccgacgg cgtcgggagc tcgtcggtga tcggcttcca ggtcgtcccg
     3001 ccgtcttcag ttttccagac ccccccggtg gccgcgccga cataaatgac gttcgtcgtc
     3061 ccgcggggga cggcgacgtc cgtgcagcgg ccgctgcaga tgtccggccc gatgaaccgc
     3121 cacgtcaatt cgggaaaggg cgtctgggct ttcagggagg cgaacgcctc ggcccactgg
     3181 agccggaggg acggatcggt cgacggggcg gcctggaccg cggccgaagc cgcggtccac
     3241 atcataacga gaaccgggat cagggtctta agccgccgca tggcttactc ttcctcccac
     3301 caagcccagg cgcggcgctt gtccttgccg ttgacgggat tggcgtccaa gacccacatc
     3361 ccccggccgt gggtggcgat gacgatcatg ttgtcccgcg gatggatgat caggtcatgg
     3421 acatacgccg agggcaggtt tccgcccagg acgttccaag tcgctccgcc gtccgtcgtg
     3481 gcatagacgc cttggtcggt gccggcatag aggatcttcg gatcgaccgg atcctcgcgg
     3541 atgacgttga ccgggcccaa gggaatgccc ttggagatgt ccacccaggt tttgccgaag
     3601 tcctcggatt tccagacata aggggcgacg tcgtcgtccc gcttgccgtt ctgggtcatg
     3661 tagacgacgg acatcttgtg ctgcgaggcg accagccgcg atacccattt ttgatagggc
     3721 aggccggcgt tgatcgccgt ccatttccgg cccccgtcct tggtcaccca gacgttgccg
     3781 tcatccgtcc cggcgtagat caggccgtct ttgagcggcg attccgacag ggcgaagagc
     3841 gtgtggtagg ggatgtcgcc catttccgac gggacggccg aggtcaggtc cgggctgatt
     3901 ttttcccagg tgtcgccccg gtcgaacgag cggaagacat actgcatgcc gtggtaaagg
     3961 atggagggat tgtggggcga aaccaggaag ggcgccagcc actggccgcg gagacgcggc
     4021 tcgtcctggt acggcacagg aaggatattc ttggaggcgg gatatcccgg gtaccggccc
     4081 ggccggctgt aatccgagcg cgtgatcgag ccgtaaaagc cggcggaata gacgatgttc
     4141 gggttcgtcg ggtcgacggc gtggttggag ccttcgccgc cgggcgcatc ctcgaagtcc
     4201 tgcggggcaa tcttgtcccg gcccccgctc acatcgactt tcgcccggta gcttccgtga
     4261 tcctgcatcg agccgtaaac gcggaacggc tcggccatgt cgtaattgat gttgaagaac
     4321 tggcagagcg ggagctcggc ctggaagtaa cgccatgtct tgccgccgtc atagctgacg
     4381 tttaagcctt ggtcgaagcc gttgagcaag tagagggaat tcgacggatc gatccagaga
     4441 gcgtggtggt cgccgccgtt cgtccggatg cggctgaagg tttttccgcc gtcgcgggag
     4501 acgttgaggg acaggcccat cgtgtagacc gtgttttcat cgttggggtc gacccggacc
     4561 tggccgaaca cccaaccgta ggtgttggag tgccgctcca tgaatttctt ttgctcgggc
     4621 gtcaggccgc tcgtctggac ccaggttttt ccggcgtcgt ccgaacgata gaccgtcgcg
     4681 cccttgatga tcccggccga ggggaggccg tacccgttga tcttttcttc ctcgctgggt
     4741 tcgcgggctt tctcgtagtt gtcgacgaac gcataaagga tgtccggctt ggagaggcac
     4801 aggtcgatcc cgatccggcc gcggtaacgg gcttcgggca ggccggcgtt gaccggagtc
     4861 caagtcttgc cgccgtcggt cgttttccag atcccgctgc cggcggagac ggcggtgttg
     4921 cggggatcgt tccatttctt gcgggtccgc tgccaggtcg tggcgtagag cgtgtcgctg
     4981 tcgcggggat ccataaccag gtcgtaagcg cccgtttcat cattgacgaa aaggaccttg
     5041 tcccaggttt ggccgccgtc gacggttttg tagacgcccc ggtcgggatt cgtcgtccat
     5101 tccgtcccgc cggcggcgac gtagacgatg tccggatttt tcggatggat gacgatccgg
     5161 gcgatggtcc ccgtcccggc caggccgcgg tgcgtccagg tccgcccggc gtcggtggag
     5221 cggtagacgc ccacgccgga ttgggacgaa cggaaaatgt tcgcctcgcc ggtgccgatc
     5281 cagacgatat cctggttgga aggagccagg gccacgtcgc cgatggccgt ggacggtccc
     5341 tgctcgaaca cgggagtcca ggtcgtccct tcattgtcgg ttttccagag gccgccggaa
     5401 gcggcggcga catacatcgt gtaggtcttt cccttgggcg tcgcgacggc cacgtcgatg
     5461 cagcggccgc tgacgttctt cggcccgagg aattgccatg tcaaatcctt gaatttcgat
     5521 tcgcccttca tcttcacatg ttgatcatag gcttgaaggc ggagggccgg gtcggtgttg
     5581 gcgatccgga ccggcttttt cgtctgctgg gggaagagga ggagggctag aatgaaaacg
     5641 gaaaaggaga gagtcaggat gcgcgggaag cgccggagag acatgagaga acctccgtcg
     5701 ttgaaatcgc ggccctcgga tcgaggatcc gaggaaacgc ggtctttcga ttttaactcc
     5761 ttcggaatct tcccgcaagg acaatgagat ttggaacgcc tctcccgata agacggacgt
     5821 tcccttaact ccaaaatgaa agaaggattg gagaaacgcc gcttctccga ggaggcgcgt
     5881 ccccaccttc ggtttgactt cgcgggggaa cctgtactac gctttcgctt tcatgaactt
     5941 gccaaatgcc ctgacttcgc tgcggatctt cctgatcccg tttttcatgt ttttccttct
     6001 ggtcgacttc aagggaaacg aatgggcggc tttcggcgtc ttccttttcg ccaccttcac
     6061 cgacacggtg gacggtcttc tcgcccggcg gaccaaccgc atcaccaccc tcggccagct
     6121 tctggatccg atcgccgaca agctcctgat gacggccgcc ttcatctgcc tcgtcggcgt
     6181 gggacgcgtc ccggcatgga tggccgtggt catcatcggc cgggaggtcg cggtcaccgg
     6241 cttccgggcg atcgccgcct ccaagggcgt cgtcatcccc gcttcctggc cgggcaagct
     6301 gaaaatgcag ctggagaccg tgacgatcgc cctcctcgtc ctcggagagg gcatcctcgg
     6361 aaaattttgg gtcgtgggaa aaatcggcct ctggctgaca atggccatcg ccttgtattc
     6421 ggcggccgaa tacttcgtca agcacggccg cgacatcgtc ctcgaaaaat agtcggagcc
     6481 gcgcctccga ttatttatcc agcgcctccg cgtcctcgat ggagcgcacg gtgaacggag
     6541 gagcccagcc cttgccgatc cgcttgatca acccgcagag gactttgccc ggaccgaatt
     6601 cgatgaacgc gtcgatctcc tccccggcca gaagctccat tgatttcgtc cagaggacgg
     6661 gccggctgac ctgacgcttg agggcgtccc gggcttcggc cccggtacgg atcatccggg
     6721 cgtcgacgtt ggtgatgacc gggaacgccg ggtcgtggaa ctcgacggca tccaagtcgg
     6781 ccgccagccg gtcctcggcg tctttcatga gcccggaatg gaacggggcg ctgaccggga
     6841 gcatgaccga tttgacgctc cggacattgc ggacgacgtc ctcgacggcc gccttgtggc
     6901 cggagatgac gatctgctcg gggctgttcc agttggcgat ctgaacctgc ccgccctcgg
     6961 cccgcccgca ggccgcccgg accgcttcat aatccgcccc caagagagcg gccatcgccc
     7021 cgacgccgac agggacggcg tcttgcatgt acgttccgcg cttgtggacg agagtcaggg
     7081 cgtcctcgaa ccggatcgtt ccggcggcca catgggccga gtattccccc aggctgtgcc
     7141 ccgcggccgc atacggcgtc cgcccccaga gacggaaggc ggccgtgctc acgagcagaa
     7201 gagccggctg ggtgttttga gtcaagcgca gcgtctcctc ggggccctcg aaacagatct
     7261 tggaaaggga aaatcccagg acgtcgtcgg cccggtcaaa cagccgccgg gcgtcctcga
     7321 aacgatcata aaagctcttt cccatgccga ccgactggga tccctgtccc gggaacaaaa
     7381 acacctgacg ggccatgcgc gactcctttt ccgtgtgaac gatggggcta ttgtatcaaa
     7441 agctcggggc ggatttcgat ctcggcctgt tcccggaggg attgaaccca gagattcgat
     7501 tgggcggcgg tcttttccac ccggatttca ccttccagaa cgtcgacggt ctcgaggaac
     7561 ggccggggag tccgtccggc ggcctgctcc gcgggaagat aacgctcccg gtaccgggtt
     7621 tcaatctcgt cgagactgac ggtcacgctc cggctgaacc gctcggcgat gacggtctcg
     7681 acgccgagct tctcctcgac atacggccgc aggtcgtctt tcccgatccc gaagcgggcc
     7741 agccgccggc ccgtctcctc ctcccccaga cgggcggtga tccgctcgat ctccgcttcg
     7801 acccgggccg ggtcggctgt tgagcggccc cggacgaggt ccaggacgac tttttggctg
     7861 atgagcttct ccaagacgtc ccggagatcc ctcccgccgc ccggcaaagc cggcgatccc
     7921 tcgacgatcc cgaacgcctc gaccacctgg acgtcgatca gggtgatgat ccggctgttc
     7981 accacggcca cgatcccgtc gacgatccgc tcccggccgc cggccgtccc gccggccgct
     8041 gaaaccagca ccgggagaag ggccagaacc aaggtcccga ttgttctcaa gcgcattttt
     8101 ttaaaacaca ttgccgatcg tcacatacag ccgaggcccg cggccgccgg ccgacggatc
     8161 aagattccat ccgaggtcga cgcgcagagg tccgagcggc gtcttgtagc ggaagccgaa
     8221 gcccagggcg tcctcaaggg catcgaagcc gacgtccttg cggctggcga agacattgcc
     8281 ccgatcataa aaaacggcca gggacaggcc gggcagggag gaaaaaggcg cgatttgggc
     8341 atcgatgttg aagaggatca aggccttccc tccgaccggc ttccctgatt tcgcgtcttt
     8401 cgggccgaga tcgtcgaacc gccggccgcg gaacgtcccc gagccgccgg cgaaaaaacg
     8461 ctcgtgaatc ggaatgcggc ccatccccag cccgctccgg aacgtcccca gcaggaggat
     8521 ccggccgccg atcggctgga aatactggaa tttcacgaag ctcttcaagt aatccgattc
     8581 gaccttgaac agcgggtacg cccattcgag gacggcgctg aaaaaaaggc cccgggtggg
     8641 attgaagctg tcgtcccggg cgtcccaaat caggctttcc gaaagagagg tggccgagta
     8701 cggataatgc tgccggtcga tctcggattc ctcgacttcc aggaaataaa gcgtcgtccg
     8761 ggcgaaccgg agcgtcgtca gggacgtcca atccggtccg atcgaccgga agccggagag
     8821 gctgaagccg cggcggtcga aaccgtagct cactctctct tcccgttcga gccaggcgtt
     8881 gaccgaggac tggaaaggca ggccgaaaaa ggccggcgat tcccacgagg cgacagcccg
     8941 tttttctttc aagctgaact ggccgatgag gctgagctgg gccgcccggc cgagcatgtt
     9001 gccgcggata aactcagcgg aaaaccgggg acggatgacg ttgttccaga tctcgaaggt
     9061 catcggctcg ttcttcgtct ccaggcccac gcccagcccc gcgtagttgc gcgctccctc
     9121 gcggaggctg acgaccagtg tttccctccc gctttcggtc gagaggattt cctcgatctt
     9181 gacctcgacg aaaactccca gtttctccag ccggcgcttg gattcgagga tcaggtcggc
     9241 ccgcgccggg tccccctcgc gcaggaggag ggcccggtcg atcacgtcgc gccgggtcac
     9301 ggcgtttccg gagacgacga tcttgtcgat gcggacgggc gtgccttcct ggatatcgaa
     9361 gaagacatcg aagcgcccgt cgtccagggc ttcggcccgg ccggcgaccg cggtcccgcg
     9421 gatccccccg tttaaatacc gggactcaag gcgctcggca tcgcggcgga cgcgcccggg
     9481 ctcatagggg ccgctctcgc ggcattccaa atcccgccgg agatcatttt ccgggacgag
     9541 cgagatgttc cggaaaacga ccgaccggat ggtccggacc ggcccctcgt cgatgacaaa
     9601 caccgcctgg tacccgtctc cccgttcccg gaattgaagc gtgaccccgg cgtcgggata
     9661 cccttccctt tcataaaggg tttccacttc ctcgggggcg gcaaacagct ggtcgccgga
     9721 aacgctgccg aaaaggggaa ggcgcggggc caacccgatt ctccgccgga tctcggcctc
     9781 ggaaaaggcc gtcaatccct cgaagacgac gctgctgatc cggattttcg gtccgggatc
     9841 gacctggtgg atgaaatgca gatcgcccgc ttccctctcg atggacgagc ggacaacggc
     9901 aaagacatgg ccttttcttc gaagggaggt caggatggcg gcctcagcct gcttcagccc
     9961 ccactcctca aaaatccgct cttcccaaag cggccggacg agagattcgg ggacctcggc
    10021 tccccggatg gtgatgcgga tccgttcccg gggatcgacc ccgagaacga ggccgacgga
    10081 cgcgtcggcc gcccggaaaa atcgggtttg gaccgcgacg tcggcccggg gatagccggc
    10141 ggcgcggtag gccgccctga tcacgtccag gtcggattca agaacggcgg gacgataggg
    10201 ccgcccggga cgcgtcgcca gcgacttgac cgccttggcc tggacttccc ccagcccgcc
    10261 gcggaattcg atgtttcgga cgctgaaccg gcgtccggcc tccacggtga actcgaggtc
    10321 gacgaaccgc cgggccggat ccggcacggc ccgccccgtc gcccgggcgt cgaaaaatcc
    10381 ctcccggcga aggttctccc gcagctcctc ttccgcccgc cgcaggcgcg gttcggaaaa
    10441 ttccgtctcg ggccggaggg cgtaaagtcc ggagagaagg cttttcctcg agacgggaac
    10501 ttcgccggaa aaggaaatcc gccgggtgaa caacttccgc gtcaaaagaa acgtcagccg
    10561 gacgtcctcc tccccttccg cccggacttc gatgtcggag aacaagccgg tttcattgat
    10621 tcgcttgacc gcctcgtcga ttcggacggg gagaaaggga tcgccgtcgg cgatcggaac
    10681 gaggctgcga aggtcgggcc ggatctcctc gccctcgacc cggacaagga cggcggaaat
    10741 gcgcgccccg gacgcgacgg cggacgccgg ccggctttcg gcggccgcca aaacggccag
    10801 cagcgccaga accgccctaa aaccttttcc ggaccttggc atcgaggctc aaccgtcccc
    10861 gctcgtcgcg catcccgacg agcgagaaat tttccaggaa ttcccattcc agccggacga
    10921 tctcctcgcg ctgggacgtg aggttggtcg aatacaagag catcagattc cgcgacagct
    10981 tcttgccgac ggtcaaacgg gccgtcattt cagtcgagga gcccaggacg aagggatcga
    11041 tccggaagcg gtccaggcgg aacagccgtt cggctccccg ggcggccgag tcggcgagct
    11101 ggaaggacat caacgatccc gtcccgacct ggctcgtggc gtcgtaggaa tacgtccttt
    11161 tgaacgattc gcccatggag agaagggcca ggacgtcctc ggccggcagc ggcggcgagg
    11221 acgtaaactc cggccggagg ctttgcagca aaccggcgac cgagagcgtc acccggtaat
    11281 ccttcaagta ggtttctccg cgaaaatcga tgtaaggctc gatcgtcgtc gggttgaaga
    11341 agctgagtcg ggctttgaga acccggaagc tccggtcttg gaagtaaacc tcgcccttca
    11401 cggcttccag gtcccctaaa acgatcggcg aggccacggt tccgctgagg gtcaggtcga
    11461 gacggccttc cattttcccc agggcgtttt cgagcatggc cccgtcgtcg gcatggatcc
    11521 ggatgtccag ggccaggtcg tcgaaaagcc ccgggccctt ccgggcttcg ggatacggcg
    11581 aggccgaaaa gatgaacttc tcgttgactt cccgccgcca gagaagccgc ttgacgtcga
    11641 cgtccccggc gaggacgtac tcccgggcgt ttttcaggaa acggaacgat ccgtcggcca
    11701 agccccgcgt tctctcgaag acggacaggg tcatgtccct tccctcggcc cggaggtcga
    11761 tcagggaaat cccgccgggc ccgaagcgga cctcgcccga tccgaagacg tcgccgccgc
    11821 cgagctttcc cctcagcgag cggatggtcg cgacgttgtt gtcgacccgg atcagtccgg
    11881 aataatcggt caaggcgtgg gcgaacttgg ggaaaggaaa gaccgctccc ttgaggtcga
    11941 gaatgccgtt ccaccgggcc ggcgaggccc cgccgccccg cacttcggcc agacagcgga
    12001 tctcgccctt ggcccccgac caaggcagga agagatcgaa gttgtagatc atcccggacg
    12061 cgccgacgaa aaatccgccg tcggtctccg ggtagcggaa agacgcctcg atcttgttcc
    12121 ggccgggatt gacctcgccc gtgaagcggg ctccaaacgc cttgttcttc atcccggccg
    12181 cggccgttcc ggtgatttcg atattctcga cggggcccac gccgaacccg gtgattttca
    12241 acagcccgga ggcttcgtcg cgggcgaagt tccccccccc tttcaggtcg aaatccagcc
    12301 ggccgcgcac ggccggggcc ggccccgaaa ggtcaatccc gcggaaggcg gcgtcgatgg
    12361 cgaaatcctc gctgtggaat cccatggcca tcgagcccga gaccgcgccg ccgtaatacg
    12421 ttccggtaag gccggaaaga gtcaggcggt agtcggccgt cgaccagccg aaccggcctt
    12481 tcacgttctc gaccgtttgg ccgtacagca cggcccgggg agaggcgaaa tcgccctcgg
    12541 cctcgatgcc ggcgccgtcg tcgtgaaatt cgaggccgcc ggagacccgg ccggcaaacg
    12601 gcagatcgag gcccagggaa ggaaagattt tttcgagcgt gccctcgcca tcctcgaccc
    12661 ggcaggccca gccgcccttt cggaccgtga accggagaag accggcaagc tccgggtccc
    12721 ggacccggac gattcccgtg gtttctccgc gggcgatctc gacgatcccc gcgccctcgg
    12781 ccgcctcaaa ccggccgaaa gagcccgggg tcagggcgaa gtcgatcttc acttccggca
    12841 cgtgccaggc gccgaggatc ttgacatccg ctctcccccg gccgcggatc tcgggaaagg
    12901 tgaaaggcat ccggagggcc aaggacgtga aagcgcggcc gcgcgggata tcggtcacgt
    12961 ccccgctgat ttcgacctga gcctcgcggc cgatatccag cctcccgtcg acccgcatca
    13021 ccgcgaaatt cgactcgatt ctctccgagg aaaacacgac cttcttggca tggtcccaat
    13081 gaaggcggaa cgacccccgg aacgggaatc ggccgttccg ttcatccgcg ggctcgtccc
    13141 ggaattcgcc ttggacctcg agagcctccg gaagcagcgt gaactccccc cagaccgggg
    13201 agaggaccgg ccagggaatt ccgatttcct tgatcaaggg atcgacgtga acgccccgga
    13261 cccggccggc gagccggccg ccgccgaacg tgagatccaa agactccacc ggcgcccacg
    13321 gcttggaaat ctcgaggttg atttttccga ccccggcgga aagagcgaga tcgccggaaa
    13381 cgcggccgac cgtctcgccg gtgaaggtca tgtcccggct ttcaaaatcc gcggtcaagc
    13441 gcgattgccc cgtttcgccg cggacgaacc gggccgtccc ggcgaccccg cccgtccagt
    13501 cgaacggcag gttgaaggcg tcggcgacga gagcgacggg cccccggacg gtcgcctgga
    13561 tgtcgatgac gggattctgc ggatggatca gcgttccccg ggccttgacg agaaggtccg
    13621 gatgatgcac cgtcagccgc cggagaatca attcgtcgcc ccgcgcctca aagtacacgt
    13681 ccgaactccc ctcaagggga tgggcgatcg ccggaacgtt gatggaatgg gaggccgcct
    13741 cggcccgaac gacgaacgcg tccttggatt ggcggaaata cgcccggacg ttcctggtga
    13801 tcaggctggc cccccgtccc cagaagctga tctccccctc ctcgagcacg ccgttttcga
    13861 cgaaaaacgg aagccggaga gtcagggagg gaggtttggc gtcccgtccc ggtttttcat
    13921 agatccgggc ggtcagcccg cggatgaaga cattgaaggc acggttgttg cggagaatcg
    13981 accggaaggg gatctggagc acgatacggt cgatggaaaa gaaggggtcc gatttcaggg
    14041 aatggacgtt ttcgaggacg gccgtgggcg gcaggaaggt cagccggaga cggtcgtaac
    14101 gcatcaccgg gtcgatctgc ttgcggatct gggagaggat gaccttgcgg acgacgacgc
    14161 ctccgcccgc caagcctcca gcgacggcca ggagggcaat caaggatttc agcttccggc
    14221 caattttcat cggtttccgc ccggcttacc gcgcaagaaa atatatcatg ttcattggga
    14281 aaaaggaaaa aagcccggac gcccgccggc cgcggtttac gcttcgccgt gaatccggag
    14341 gacgcggttt cggagcgttc cgatcccctc gatctcgacc tcgacttcgt cgccgggaac
    14401 catcgggccg atcccggcgg gagttccggt cgcgatcacg tccccgggaa ggagggtcat
    14461 gacgttcgaa atgaagcgga cgaggtccgg cacgctgaaa atcaggtttc gggtgttgga
    14521 ggactgccgc agcttcccgt tgaggcgcgt ccgaatctcc aaggccgagg gatcgaggcc
    14581 ggcggcgatc cacgggccga tcgcggcgaa cgtgtcgaag gacttggccc gggtgaactg
    14641 cttctccttg tcctggagat cgcgggccgt cacgtcgttg aagcaggcgt atccgaggat
    14701 gaaggattca accggatcga gggcggaaag ccgggaagcc cgctttttca tcaccacggc
    14761 cagctctccc tcgtagtcga cccgccggga catccgggga tagacgatcg ccccgtccgg
    14821 accgatgacc gccgtggacg gtttgaggaa cagcagcggc tcttccggca gcggcttgcc
    14881 cctctccgcg gcatggtccc ggtaattgag cccgacggcc acgatcttgg acggcccgac
    14941 gggggcgaga agctggactc cggccagggg aatcggctcc ccgccgacga cgaaatcggc
    15001 gaaaatcgat ccctcgatgg gaatcaggcg gtcgtcatcg acggctccgt gcagaacgcg
    15061 atccttgaac cgggcgcgag cgattttcat gatccggctc cctttcccgt ctcggtcaag
    15121 acttcggacc ggccgctttc attgcccgtc cggtcgaggg cggtgacggc gtactggtac
    15181 cgtccgcccg cctcgacctg gtcgtcggtg aatgtgttct cgctgatcgg ggcgggcgtc
    15241 atgagggaaa aaccggtctc ccggggccct ttccgccaga cccggtagcc ggcaagatcg
    15301 gtctccggtc cgggatccca agacagggtg atcaagccga cggccgtcac cgagatgagg
    15361 cctttcggcc gggcgggcgg aaaggagtcg gccggggtca cttcgagagc ctcggaatcg
    15421 cggctctcga tgaacggctc tttctccccc gcgacggctg aaacgcggta ggcataggaa
    15481 acgccgaagc ggaaggtccg gtcggcgaac ggcggcgccg gcaccggggt ttcgttcaac
    15541 cggacctccg gaccgccggc cgagctccgg tacacgttga agccttgccg cggcggcgtt
    15601 ccggcggcgt ccttcggaac gttccagccg agctcgatcc ggtcctcgaa cacccgggct
    15661 ttcaggcctc cgggcggggc cggcgcctgc aggggcagcc agcccgcata cgcgaatttc
    15721 gacgcgcccc gtcgtccggc ggccactctc acgccgaaaa ccaggcgggc cgcggccccg
    15781 tcctccgggg tcatctccct ctcccaggag aattccttaa gcgggggagc cgccggcggc
    15841 ggaaccgccg ccgtcccggc ttgaagggga cggccgtaag agtcgaggac ggacaggaga
    15901 acggccttgg ccgtgaattc gtcttcatcc tcccaggcca tgggaagggc cgcttcggcg
    15961 gccgcgttcc gcatcagcca gatttccgtc cgcgcgtccg gaggcagggc ccggccgtcg
    16021 atgtacgccg cgggggcggt ccaccggagc cgcagcgtcg ccccccgctg gccggcggcg
    16081 agcgaggcga cgggttgggg gatgcgggtc aggggctcga ggatgggtcc cttgcggccg
    16141 caggcggcgg ccgccaggat cgccaagccc aggaccgtcg ccggccggat tttgcccatc
    16201 agaggatgaa ccgacggagg tcggcgtttt tgacgatgtc ctggagggcg ttccggacgt
    16261 acgtccgatc caccgtgatc ttcttcgcgg cgatgtccgg cgcctggaag gagatctcct
    16321 cgaggacttt ctccatgatg gtgtgaaggc ggcgggcccc gatgtcctcc gcctcgcgat
    16381 tgacgtcctc ggccgtttgg gcgatttcgt cgatcgcgtc ggccgtgatc gtgatctcga
    16441 cgccctcggt ctccaggagc gccttgtact gcttgatcag ggcgttctcg ggctcggtca
    16501 agatgcggac gaagtccgcc ttattgagcg aggtgagttc cacccggatg gggaagcggc
    16561 cctgaagctc ggggataagg tcggacggct tggccacgtt gaaggccccg gccccgatga
    16621 acaggatgtg atccgtgcgg acgaggccgt agcgcgtgtg gaccgtcgtt ccctcgacga
    16681 tcggcaggag atcgcgctga acgccctcgc ggctgacgtc gggcccgcgg ccggattccc
    16741 ggccggcgat cttgtccagc tcgtccaaga aaacaatgcc ggagcgctcc acccgctcga
    16801 cggcgagacg ggcgaccagg tccatgtcca ccagccgccg ctcctcctcg tcgagaaaat
    16861 actcccgggc ctccgaaact ttgactttgc gttttttcgt ccgcccgccc atgaatcccg
    16921 gcaggatttc ctggatctgg agaccgattt cctcgacccc ctggttggaa aagatttcca
    16981 agggaggcgt ggagcggtcc cgcacctcga tttcgacgat ccggtcctcc agaagcccgt
    17041 cgcgcagctg ccgccggagc ttctcccgcg actccagata ccgctggtgg tcctcgtcgg
    17101 agacgacgcc gttgcggcgg cggaccggcg ggagaagaag atccaggatc ttttcctcgg
    17161 ccccggccga ggccttgtcc cggacttcga gggttttctc ctggcggatc atgtccacgg
    17221 ccatgcggac gagatcccgg acgatcgatt cgacgtcccg gcccacgtac ccgacctcgg
    17281 tgaacttgct ggcctcgatt ttcaggaagg gcgagacggt cagcttggcc aggcgccggg
    17341 agatctcggt tttccccacg ccggtcggcc cgatcatgag gatgtttttc ggggtgattt
    17401 cgtcggccag gtcggccggc acgcggcgcc ggcggtagcg gttgcggagg gcgacggcca
    17461 cggctttctt ggccgcgttt tggccgatga tgtacttgtc cagctcggcc gtgatctccc
    17521 ggggcgtcag ctcggcctcg cggtcctttt cccggagctt atccaacggg ccggccaggc
    17581 ttttcggcag atgaatggtc attcagattt cctcgatcgt gatggtgtcg ttggtgtaaa
    17641 cgcagatgga cgcggccgtc cggagggcct cctcggccac gtcgcgggcg gaaagcgtcg
    17701 tgtgtttcag caggacgcgg gccgcggcca aggcgtaggg accgcccgag ccgatcgcca
    17761 gcacgccgtc gtcggattcg aggacgtcgc ccgtcccgga gatcatgagg gccgaatcct
    17821 tgtcggccac gatcatgaga gcttggagct ggcggagggc cttgtccagg cgccagtcct
    17881 tggccagctc gatggccgcc cggtacaggt tgcccttgta ctcctccagc ttcgtctcga
    17941 accgggcgaa aagggcgaag gcgtcggacg tcgagccggc gaacccggcc gccacctggc
    18001 ccttgtgcag gcggcggacc ttgctggccg acgatttcag gatggtctcc ccgagcgaaa
    18061 cctggccgtc gccggcgatg gccgttcgtc cgtcgcggcg gacgcagacg attgtcgtgg
    18121 agcggatcat gtcggcctcc ctgggtcgaa acatcaatat aagtcaaggc cccggccgcg
    18181 tcaatttcgg atatccgggg aaaggcccct cgagaaccaa gcggggggcg gctccaatcc
    18241 ggcgggttca ttcgccccga ggagccgttc ggccggttga actcggggct ctcttcgggt
    18301 actttatcct tcgaacgtca aaacggatca ctcaaggagg accgcccatg gctcgaaaaa
    18361 tcggacgccg ggaattcatc cgctccgggg ccgcgctcgg cgccgcggtc gtcgtcggcg
    18421 gaaagctgtt cggaaaatgg accgggaatc cgccgtccgg ggaatccgga ccgtcggtct
    18481 tcgtcgtcaa ggggacggac tcggcggcga tgacggccaa ggccgtcgag ctggccggcg
    18541 gcctcggcgc ctacgtcccc aagggagcca aggtcgccct tctgccgaac gtccaaagcc
    18601 ggcatcccgg gacgttcacc aagcccgaaa ttcttcgggc cgtcctccgg atgtgccggc
    18661 aagccgaagc ggccgagatc gcctgtctca gctggctcgg tccgacgaac tgggacggca
    18721 ccggcctggc ccgggtcatc gaggaggaag gcgccgccct ccggctgatt ccccgggagg
    18781 atgaaaactt caaatccgtt cccatccccg gggccgtcgc cctgcccgag gcccggatca
    18841 tgaacgcctt gttcgaatac gacctctgga tcaacatgcc gatcaccaag gaccacgccg
    18901 ggaacaaatt caccgggaca atgaagaacc tgatgggcct caacgccccg tcgtccaatc
    18961 gcgcccaatt ccacaagccc aattggacga cggatccggc ggacatcgcc cacttggaat
    19021 attgcatcgt cgacctcaac ttcgccgtcc ggccggcttt caacatcgtc gacgcgaccg
    19081 agatcatccg gaccaacggt ccgatgggcc ccggcgagct gatcaaaccg ggccggatcg
    19141 tggccggggt cgaccgggtc gcggtcgacg cctactgcgc gacccttctc gggttccagg
    19201 gcgcggagat tacggccatc aagcacgcgt ccgagcggaa gctcggcgag atcgacctcc
    19261 ggaaagccgg cgtgctcgag gttgagatct aagggaagcc gcatgaaaaa ggccgccgcc
    19321 ggtccgctcg ccgccgttct tttggccgga tttttccagg gaggcgggac ggcttcgata
    19381 ccgtttcccg ttccggattc cgacccggcc gcgctcatcc ccgcggccgg cgaagttccc
    19441 ggctggacgc cccggggcga ggcccagcgc ttcgacggcg aagacctcta tgtctatatc
    19501 gacggcggag cggaaatctt caacgagtac ggcttccgac gggtcatcgc ccaggatttc
    19561 gaaaatccgg gtggagccgg aatcgccctc gagatctacg agatgacgga tccggccgcc
    19621 gccttcggca tctattcgtt tcaaaggagc ggccaaggac aggcggccgg cctggggacc
    19681 gagggggaga tcgaggatta ttatctccgc ttctggaaag gcccctattt ggccgtcgtc
    19741 acgggatacg atccgcacgg ccgggatccc ggacgcctca tggagggcgt gatcgccgtc
    19801 gcccgggccg tggacgcccg gatccaggag tccggcggcc ggccggcgtt cgtcgaggcg
    19861 ctcccgccgg attggatgga acccggcctc aagtacctgc ggggcgtgtt ggggctcaat
    19921 aacctggttc gtctatttcc gcgggatgtc ttgaggttcc gggaggcggc cgccgtcccc
    19981 atcgaggggg gctggctgtt tgtctgcgga taccgggacg cggccgaggc cgaaagccgg
    20041 ctggccggag tccgggaggc gatgtcggcc cgagagggcg ggtacgggga gttccaggcc
    20101 tttcccgacg gccgactcga ggcggccgat tccaggggca atcggatcgt ggtccgggcg
    20161 gccggggaca agatcggcct ggttgtctcc ccccgggcgg cggccgtcgc cgaggcgctt
    20221 ctcgagcggc tccgttgacg ggagtttggg ggttttgatc ccggcctggt tgtgatatag
    20281 aataaggaat ctatttcaga aaagcggagg cggttatgcg cgtccggatt tcggcggcta
    20341 tcgccggttt cgcggtcatc gggagcctgg gcttttcggc ggccggcccg gccggttcag
    20401 cggatccgct cagggcggtt ctttccccct tgaacccggc ctttgtccgg gccttggaaa
    20461 gaggcgacgt ccgccatccc agtccccagg acatcactcc cctccaggga ttgctcccgt
    20521 ttgtggatcc gaccctgcca tccgcctacg atctgaggga tgagtaccgg gtcaccggcg
    20581 tccgcggcca gtttcccgtc gtcacctgca cgattttcgg ccttttcgcc tcgctcgaat
    20641 cgactttaaa gccctatgaa acctgcgatt tttccgaaag acacttggac ggcggctccg
    20701 aggaaagcga cgccctcgag gccaacgtcg gggcgatggc ccggtggtcc gatcctgttc
    20761 tggaggatga cgatccgtgg gagggcggga cgccctacga cccggcccgg ccggcggtca
    20821 agcatgtcca gaaagtcgtc ttcctgcctc cccgggccga cgccctggac aacgaccgga
    20881 tcaagagggc cgtcatggat cggggggggg tcgcggtcga gatgcgcttc agccggtcct
    20941 tgctcaacga cacgtacgat tcttattaca cgacggtgga gacgcccgac gtccacgccg
    21001 tcgcggtcat cggctgggac gatcttttcg ccaaggaaaa attcacgccc cgagcccccg
    21061 gcgacggggc gttcatttgc aagaacagcc tgggcggcaa tttcggcgcg gccgggtatt
    21121 tctatgtgtc ctattatgac gcgtttttcg gccgattctc cctccccgcc gtcgtcctgg
    21181 ccgaaccggc gtccgggctg attgaaaact atcaatacga tcccgtgggc tgcacggccc
    21241 gcctgggctt cagctcggaa acggcctggt tcgccaacca gttcgtcgcg gtctcgaatg
    21301 atcccctggc cgccgtttca ttctactcct acggcgaggc gggcgaatac gaggtcttcg
    21361 tctacacggg cgccgcgccc ggcctgcccc aaagcggaac cctggcggcc caattcgcgg
    21421 ggacgctgac cgaggccggc taccacacgc tccgcctgcc ggaaccggtt ccgctggcgg
    21481 tcaacgagcg gttttcggtt gtcgtccgcc tgcgcagcca gggcaacaat tatcccattc
    21541 cgatcgagca tcccgtcccc ggcttcgacg ccgacttcaa cgcccttccc gggcagagct
    21601 tcatcagctc cgacggccag atctggagcg atttggtgac atacgaagga ccgacatacg
    21661 cccgctcgaa cgtctgcctg aaggcgttcg ccggctatgc gcccgtgtat ccgccggctt
    21721 atctccgcgt cgagcggctg gtcaatgacc tgatcttcta caaggagtac gtcgaccggc
    21781 tgtcctgggc ggtccatccg tccaacaccg aaccgatcgc cgcctaccgg atctaccgga
    21841 aagaaaaggg cgcgcccaac gactcgtacg tcttcctggc cgagaccgga acgacggacc
    21901 tgtcttatta cgtccgcggg ctcacggcaa ccagcgccta cgcttatcgg gtgacggcgg
    21961 tcacggcctc cggccgggag agcgatcctt ccgaagtcgt cgattgacgg ggcgggaatg
    22021 aaaaaaaccg ccctgtcgat cgtcatcctc ctcgcgtggg cgggactgtc cccggcccaa
    22081 acctgggagg cggcggtggc cggcggctat cggacgctca aggactccgc cctccgcgaa
    22141 atctacggca acggcttcgt cgtcgctccc agcctcagcc tggcgatgtc aaaaaccctc
    22201 cgggtcgggg ccgagtacga gttcgggtac tcgaaaaacg cggccatcgg gatgttcgag
    22261 gatccctcca ccctcaaggt ccgcggcggt catcttttcg ttcagtacgg cgagcgatcg
    22321 ggacggttcc gtccgtttat aaagacgggc gtgggaatct tcgcctacca attcgacgtc
    22381 cgaacccctt cccaaccgac cgtgaacgtc tctgaaacgg acgtttcgtt cttcatcggc
    22441 ggcggcgtgc gcgcgttctt cacgaaacgc ctgttcggga cggccgagct aaaatacgtc
    22501 gcgttgtggg tgggctcgta cgacgacaag gtcgatttgg ggggaatccg gcttctgctg
    22561 ggcgtcggct acgacttctg aatgaggggg ccgcttccgc cgaaaagcgg gacggtggag
    22621 cgatggcctc ccctctccgg gccggctttc cttatgagga ttaaaaaacg ttgtattcgt
    22681 cgagggtttt gcccgtccgg aagcgctcca tgcggagaca ggaaatatcg aggctctccg
    22741 tccggccctt gaggatctcc tcggccgcca gccgtccggc cgccgggctg tgcatgaaac
    22801 cgtgtccgga gaaacccgcg ttcatgtaga atcccttcag ctcctccacc ggcccgataa
    22861 tcgcgtgatg atcgggcgtg acctcataaa ggccggccca gcgccgggcg atccccttct
    22921 gctccatgag cggcaggcga aaggcggccg tttccaggtg ggtcagctcc cactcctcgt
    22981 cgacggcttg gtcgaacgag ggcggctgat cggcccggga catgccggag agcagaccgg
    23041 ggccctcgcg gtgaaaatag aggcccgttc cgaactcgat gacgaacgta aaatcggccg
    23101 ggatttcggg cgtcggggtc gtcgtgaaaa gctggcgttt gaccggctgg acgggaacct
    23161 ccagaccggc catccgcccg atttcccccg cccaggcgcc ggccgcgttg accaccagcg
    23221 gcgttgggat cctgccggcg ctggtcagga ccgcttgaac ccggccgccg gccgtctcca
    23281 ctccggtcac ctccaccccg gtcaggcagc ggacgcccag ccgggaggcc gccgaaacgt
    23341 atccctgaac gaccgcgccg ttgtcggcca gtccgtcgtc cgggccgaac aggccgccga
    23401 gggcgtcctc gaaattcatc atcggaagac ggcggcggat ctcgtcgccc gtgagccact
    23461 ccgtccgcag gccgagggcc aggtgcatgt ccatctgccg ctggaaggcg gcgacgtctt
    23521 gttccttgtt cagaacaaag agatagccga ttttgcggta atccgccgaa taccccgttt
    23581 cttcctcgaa ccgctcgaag gccggaaggc tcagggccga catccggcag ttgatctcgg
    23641 tgtcgaaatg ggcccggacg ccgccggcgc acttgcccgt cgattcggtt ccgaagagcg
    23701 gctcgcgctc gagaaggacc acgttcttcg ccccgccctt ggccagatga tacgccgtcg
    23761 acgccccgat gacgccgccg ccggcgatga cgatatcggc tgtttcagga agatgcatct
    23821 cgttcacctc gatctcgatt ttttatccgg gctcgcgatc cgggccgagg aaccgccgga
    23881 agaatcggcg gtgaccggtt attatcgccg acttgaacgc ccggcgcaag tttctatata
    23941 atcacggagg atttgttcat gaaacgcggt tcgaacgccc tccttcaatt tttcctcttt
    24001 ccttgatcgg ccggtgagtt gttccgcctc gtttcaaggg ccttccgcgc gttaaaatct
    24061 gtttcacggg aggaaacgaa tgcctgtgtt ctccaaaacg ttccttcgcc tcttcctgtg
    24121 cgccctggtt ccggccgttc tggccgggac cgtcgccgcc cagggaccgg cggccggatc
    24181 cgaccggccg ctgttccggc atctttcctg gcgctccatc gggccggcca acatggtcgg
    24241 ccggatttcc gactttgaag ccttggacgc cgatttcacg cacgtgctgg tcgcttccgc
    24301 ctccggcggc gtcttcaaat cgatcaacgc cggaacgacc tgggaaccga tcttcgaccg
    24361 ctacggctcg gcctccatcg gcgacgtcgc ttggtttcag aaggacccgg acatcatctg
    24421 ggtcggcacc ggcgaggagt gttcccgcaa ttccgtcgcc tggggcgacg gcatctacaa
    24481 atccaccgac gggggcaaaa cgttcacccg gatggggctg gaaacgaccc acagcatcgg
    24541 gcgggtcctc acccatccga ccgaccgaga caccgtctac gccgccgccc cgggccacac
    24601 gtggggttat tccggcgacc gcggcctgtt caagaccgtc gacggcggcc ggacttggat
    24661 caagctgggc ggcggcctgc ccaatgacgg aaagacggga gcgatcgacc tggtgatgga
    24721 tccgacgaat cccgacgtcc tctatgtcgg cttttggcag cgcctccgcc gcccgtggcg
    24781 gttcgattcc ggcgggccga acggcggcat cttcaaatcg agcgacggcg gccggacttg
    24841 gatcaagctg acccggggac tccccgaggg ggacaccgga cggatcggct tggccatctc
    24901 ccggtccaac ccgaaggtcc tcatggccag cgtcgagcac ggctaccagc cccgcccgac
    24961 gatccccggg gaggccggaa agccgcccaa agacaatccc gattacaagg acatgaccaa
    25021 gctcggcacg ggcatctacc gctccgagga cgggggggaa tcgtgggtgt tcgtcagccg
    25081 gatgaacaac cggccgttct actacagcca catctacatc aatccggtcg aggaccaatg
    25141 ggtgtactcg ctggccacga tcatgaattt ttccagcgac ggcggccgga cctggacgcc
    25201 gatggaaggc ctccatcccg actttcacgc gatgtggctc gacccgacaa acaagaaccg
    25261 gatgtacgtc ggccaggacg gcggcgcctt cctgtcccac gacaacggaa agacctggat
    25321 catcttcgac aacctgtcct tcgcccagtt ctacgccgtg agcgccgaca tgcgcgatcc
    25381 gtattacgtc tacggcggcc tccaggacaa cggcacctgg ggcgggccgt ctctccaccg
    25441 cgaaggctcg atcctgaccg atttctggta caacgtcggc gggggcgacg gcttccacgt
    25501 ccagatcgat ccgacggact ggcgcatcct gtacagcgaa agccagggcg gggcgatcag
    25561 ccggtggaac gtcgaaaccc gggaatccaa gtcgattcgg ccgaaccggg gcaatatcgc
    25621 caactacaag gattatttct ccgatccgcc cgagccggag cgcaagccgg gggcggaaaa
    25681 cgtcccgcct gccgatcccg aaacggcctg gcgggagatg gcccggcgga tgcgcgggcc
    25741 gttccggttc aactggagca cgccgatcct tctttcgccc cacaatccgc ggactctcta
    25801 tttcggcgga aaccagctct tcaaatccac cgaccgcggg gagaagtgga tgatcatcag
    25861 ccccgacctg acggccaacg acgcggagaa aaacaagccc acgggcgggc tgaccgacga
    25921 cacaaccggg gccgagacgc acggcacgat catttcgatc agcgaatcgc gcctgcggcc
    25981 ggggctcctg tgggtcggaa ccgacgacgg aaatgtccaa atctcgcgca acgacggcgc
    26041 gtcctggacc aacgtccggc cgaacatccc gggcgtgccc gccggcctct ggtgcagccg
    26101 ggtcgaggcc tccccgttcg acgaggggac ggcctacgcg gcgttcgacg gccaccggag
    26161 cgatgatttc cgggtctacg tcttcaagac gacggattac gggaaaacct ggacgtccat
    26221 ttccaagggg attcccgacg gccagccggt ctatgtgatc aaggaagatt taaaaaagaa
    26281 aaaccttctc tttctcggga cggagttcgg cgtgttcgcc agcctcgacg gcggccggag
    26341 ctgggactcg ttctccctga acatgccgac cgtggccgtc cacgatttat tcatccatcc
    26401 ccgggagcat gacctgatcg cggccaccca cggccggggc ctctggatca tggatgacct
    26461 gtcggccctc cgccaggccg tcgaccttcc cgcctcgacc gaggcggccg ttttggaggc
    26521 ggggcggccg ggaacgaaat ggctgaccat cgcccgcggc ggatacggtc ggggcgatct
    26581 gtacttcaag ggagagaatc ccccccaggg ggcgatgatc cactactatt tcaaggaaaa
    26641 accgcaacag ccggtctcgc tggaaatcac ggacatcacc ggccgtcgga aaacggtcta
    26701 tatcctcgac gacatccaac ccggcgtcgg ccggcttgtt tgggattttc tgttcgatcc
    26761 ggccccggcc gccgttcaga gttccgccgc tgcgctgaaa acccagctgg aggccgcccg
    26821 ggagcggacc gaggcgacgg cggagcagaa agcggcgatc gccgccgctc tgaacgagct
    26881 cgatgcggcg ggcggcaatc accggaaaat tcaggaggtc cggcggaaat cgatgagctt
    26941 gatcggacgg gggatgttcg gccgttacgg cggccggtac ccgggcgggc cggggatggc
    27001 gaatctcgaa gccgaagccg gcaagtacgc ggtgaagctg atcgtcggcg acaaaacgta
    27061 tgccggggac atcgacgtcc gtctcgatcc gcttaattcg gaacctggtt aaccatttcc
    27121 cgaattcgaa atcggttaat ggttaaccag gcaccgattt catccctggt tattttttca
    27181 gtccggcgtt gagggcgtcc agggccttct ggggattcat cgccccgaaa tgaaacagaa
    27241 gctcctcccc cgccttcagc ttcacggcca gggattttag taaaaatccc ttcttgacga
    27301 tctcaccgcc ggcgatctcc gaaaagggga tcgtcagatg ttcctcggat ttctcgaagg
    27361 ccttgatgtg ttggtaattc gtcatcaagc tcgccgcgtt tttctcgagc gacaagccgg
    27421 cggcgaaatg aaaattcaag ttcgtgatgt ggatttttcc ggtgaccttc gcgtccatcg
    27481 ggccctgacc gccgatataa accgaccagg atccgatttc ttcctcgccc ggctgcagcc
    27541 agtccttgcc catgggattc ctccttcgcg cggcgcgttt ccttcttccg ggatcaactc
    27601 tattcccatt tgaaaagcga agtcaaaccc gaatccccgt cggccggaaa acgcaagaac
    27661 tcggaaaacg ccgcagggac ttatccgact tctcccctcc gcattcgctt aagccagggc
    27721 cgccccgtcc cccgctgaat ccctgattcc gaacctgctt caattccgaa cctgtttaac
    27781 catttcctta tttcgaaatg cggaaatggt taaacaggtt cagatttatc attctcaggg
    27841 aatatcgcaa gaaagctctt tttcatcatc atttcatccc cgtttcacta tttcttaaca
    27901 catcatgatt attcttcaac cgacatgaaa accacacaat tccaaggaga aaaaaagaac
    27961 atgagtttga ggaaaaggtt gatcctgacg acggcggccg tcttgctgac cggcttggcc
    28021 ggtttcgccc agcagacctt tcagacggcc gtttacttcg actactcgca tttcatcacc
    28081 accgacggtt acctgacgtc caacacgttg accaacaagt tccacttccg tcgcgcgtat
    28141 ttcacctacg aaaacaagat caacgaccgt ctgaagtttc gtttccgcta cgacgccgac
    28201 aacacggcca acctcacctc ggtcgacttc gccaagcaga cggtgaagaa ggacgataag
    28261 ctccggcctt tcgtcaagca cctctatctc gagtggtccc cgaatttctt ggcatccaag
    28321 gtcaatgccg ggatgatcga aactctggct ttcaaactgg ccgaggaccg ttggggttac
    28381 cgctccgtgg ccaagacgct cctggacggt tacaaggaca tcaccggcgt cgacgtcgac
    28441 gccacttcgg ccgatctggg cttgagctgg aagggaaccc tcctccggca gctgcgattc
    28501 ggcctgggcg tttacaacgg ctctcattac tcccacgccg aaaccgataa attcaaaaag
    28561 ttcagcggct acctgcagct cgttcccgca gccgggctga gcctcatcgg ctacgtcgat
    28621 tacgagaagc agaccggcga ccggaaggcg atgacgacca aggtcgacgt cctcttcgac
    28681 atggtccgca acctgaacat ctccttcgag tggttcgcct acgacagcga caacctgctc
    28741 aattccaatg gaacccattt caagccctcc ggatggtcgg ttttcggaac ctacaagatc
    28801 aaaccggaca agctggccgt tttcgcccgg tacgaccggt acgagcccaa tgacaccaaa
    28861 gccgacgacg agatcggcct gatcatcgcc ggattcgact ggaccccgat gcacgggagc
    28921 tggaagctcc agcccaacgt ctggatctac aattacaacg acgcccggaa aggcgacacc
    28981 atctttaatc tgaccttctt cctgagcttt taaggaacgt caataaagga gacaaaatga
    29041 tgaatgagaa atggattaag gtgctggttc tgacgggact ggtcctagcc gtgatcttca
    29101 cgccgatcct gctttccgcc cagaccaaga tgctccaggt caagggctcc gacacgatgg
    29161 tcaatctggc ccagatcctg gccgaggaat tcatggccca gaacccgaaa atgccgatcg
    29221 ccgtcctcgg cggcggctcc ggaacgggca tcaccggcct gatcaacgga acctgcgaca
    29281 tcgccaacag ttcccgcgac tggaaagcca aggagctcaa tctggccttt gaaaaaggcg
    29341 tcaagccccg gctcttcgcc gtggccgtcg acggactgag tatcatcatc aacgagaaaa
    29401 atcctctgac caagctgact atggaacagg tcggggcgat ctatcgtgga gaagttaaaa
    29461 actggaaggg cgtcggcggc ccggatcaaa agatctccct ctacggccgg cagtccaact
    29521 ccggcacgta cgccttcctg atggaacacg tcatgggcaa taggccctac tcgaccgata
    29581 tgaaagaaat gaacggcaac gcccaaatca tcgagggcgt cctcgccgac gcgagcgcga
    29641 tcggctacgt cggcgtaggc tacgtcgtcg acaagaacac cgggaaggcg ctcaagggct
    29701 tgaaaatcct cgacatcagc caggcgccga acgggaccgc ttattccccc ctggacaaga
    29761 cggccgtcga ttcgggccaa tacccgatct cccggcctct ctggatggcc gtgaatggaa
    29821 ctcccaagga cgtcgccgcc gccttcctcc ggtacgccgt cgggccggaa ggccagaaag
    29881 tcgtcgaacg cgagggtttc tacaccgtcg gaggccggct ccaggaacag aatcagaaga
    29941 acctcaagtg aggttcgcag ccatgaagag gcgcggatcc ggggctaagg tccccggacc
    30001 ggacctcgga gaggcgtccg acgggacgcc tctccaaact tctctctccc cgctgcgagc
    30061 cccgctgttg aatccggaaa gaggtcgaac atgatcagtc gccgactgcg aaacctcaag
    30121 gaacgggcga tccgggcttt ctttttcctg aacggcatcc tggtcgtcgt catcctggcc
    30181 gggatcttcg tcctcctcct ctcttcggcc gtcccggcct tccgggaaat cgacctgaag
    30241 gaatttctct tcggcgcgct ctgggatccc acctccccga tccgggcgag ttatggcatc
    30301 ctgactcaaa tttacagcac cttcctcgtc acgctcggcg cgatgatcct ggccgtgccc
    30361 gtcggcctgg ccgtggccgc ctacttgtcc gatgtcgccc ccgggcgggt ccgggaaatc
    30421 atcaagccgg tggtcgagat cctggccggg gtcccttcgg tcgtcatcgg attcctggga
    30481 atcgtcgtcc tgggcccggc cttgagcaag ctgaccggcg aaagccacgg gctgaacgct
    30541 ttaaacggga gcatcctcct ggcgatcatg tcgctgccca cgatcatcag catctcggag
    30601 gactcgctcg cggccgttcc ccggtcgtac agcgaaggat cgctggccct gggcggatct
    30661 cgctggcaaa cgcttctccg ggtgaaattc ccggcagccc tttccggcat catcgccgcg
    30721 gtcatgctgg gcatgggccg ggcgatcggc gagaccatga ccgtcttgat ggccaccggc
    30781 aacgcccggt ccttccccca cggcctgttc gattccgtcc ggacgatgac ctcaaacgtg
    30841 gccatcgagc ttggtgaagt cccttacttc accacccatt actacgctct cttcgccatc
    30901 ggcctggttc ttttcctcct gaccttcttg atcaacctgg cggctgatag ggtcctgcac
    30961 aagtaccggg agcgcgaacg atgaaaatct cactccgcct gaggaaagcc gtcgcccagt
    31021 ccctgggctt tttcggcctc cgggtcgccc tgggcctcgt tctgctcgtc attgttttca
    31081 tcctggccga catcggaacg aagggctccg gcgtgatcag ctgggaattt ctctcccggc
    31141 ctccccgcag aggcatgacc gaagggggca ttttcccggc catttacgga acgctgctcg
    31201 ttaccctgat caccgccgtt ctcgccgttc cgttggggat gggcgcggcc gtctatctca
    31261 acgagtacgc cccgaaaaac aggctgacgc gcctgatccg gctttcaatt cggaaccttg
    31321 cgggcgtgcc ttcgatcgtc tacgggctgt tcggcgtcat tctcttcgtc aacatcttca
    31381 agttcgggac ctcgatcctc tccgccggcc tgactctggg tctcctgacg ctgccctgga
    31441 ccatcaccgc ctcggaggaa gcgttgaaga ccgttccgaa ttccttccgg gaaggagcct
    31501 tagccctggg agccaccccg tggcagtgca tccggaccaa tgtccttccg tacgccgtgc
    31561 cgggcatgct gaccggaacc atcctcggcc tggcccgggc ggccggagag accgccccga
    31621 tcatgttcac cggagccgct ttcgcccttc cgatcctccc ccagtcgatc cgggaccagt
    31681 tcatggcctt gccgtaccat ttgtacatca tggccaccca gcatcacgac atcgccacgg
    31741 tccgccctct agcctacggg acagccctgg tcctcatcgt tctcgtcctg ggcctcaacc
    31801 tcggagcggt cctcctccgc taccgcctcc gccgccggca caaggaatac tgatatgaac
    31861 tcccgtccga aaatcgaaac gaaaaacctg aacttctttt atggttccaa gcgcgccctg
    31921 aagaacctta acatcgcctg ccataaaaaa aaggttacgg ccatcatcgg gccgtcgggc
    31981 tgcgggaaat cgacgttcat cagaaccctc aaccggatga atgatgtcat tccgggaacc
    32041 cgggccaccg gcgacatcct ggtggatggg gccggcatcc tcggaccgaa agtcgacgtc
    32101 gtcgagctgc gcaaacggat cgggatggtc tttcagaaac ccaatccctt tcccaaatcc
    32161 atcttcgaca atgtcgctta cggcctgcgg atcaacggga tggggaaatc aagggagaaa
    32221 acggccgaga ttgtggagaa gagcctccgg gaggcggccc tctgggacga ggtaaaggac
    32281 cggctcaaag agagtgcctt cgccctgtcg ggcggccagc agcagcgtct gtgcatcgcc
    32341 cgggctctgg ccgtcgagcc ggaggttatc ctcttcgacg agccctgctc ggccatcgat
    32401 ccgatcgcca cggccaagat cgaggacttg atcgcccgct tgaaaaagac atataccgtg
    32461 gtcatcgtca cccacaacat gcagcaagcg gcccgtgttt cggattacac cgcttttctg
    32521 atgctcggcg agctcatcga attcgacgag accaaaacaa ttttcacgtc tccggccaat
    32581 caaatgaccg aagactatat caccggccgg ttcggataag agcatttgcc ccgggaacgc
    32641 atttgggata taaaaaggcg gatagggact ccctcatgaa cgagaaaatc gccgtcgtcg
    32701 aggacgagcc ggatctattg cgtttgacgg ccgaggcttt aggccgggag ggattcaaag
    32761 tggcggcctt tccggatgcg acgagctttc tccgctctct cgacaaaacc cggcccgatc
    32821 tcatcctcct cgacttgatg ctgcctgacg ccgacggcct cgacgtctgc cgggccatcc
    32881 ggggccatga tgcgcgccga tcgatcccga tcatcatcat gacggccaaa ggcaccgaaa
    32941 cggatcgggt tctcgggctg gaacttgggg ccgatgatta cgtcgtcaag ccggtcgcct
    33001 ttagggagct ggccgcccgc gtccgagctg tccttcgaag ggggaaggcg gacgcagcgg
    33061 atcagaaaat ctcccttgga aacggtgaat tcacgatgga ccccgaacgt ttcgaagcac
    33121 gtctttcggg gaagccggtc gagctgacga cgacggaatt caagcttctc caccttctcg
    33181 cctccaagcc cggccgtgtt ttcacccgag atcagatcct cgatcgactg tgggggaacg
    33241 ataaaatcgt cctcgaccgg acagtcgacg tccatatcaa gaaccttcgg gaaaagctcg
    33301 gtccagccgc ccgattcctc aagaacatcc gaggcgtcgg ttacaagatc gataattaag
    33361 agagatcgaa ccgcgtcgac ttaatcccga tgaagcgttg tcttgttcct ccgctgaagt
    33421 gaagcgccgg ccggccgttc tttaaatcat ttccgccgcc aatccgaagc cgttcttttc
    33481 aattccgaac ctgtttaacc atttccttat ttcgaaatgc ggaaatggtt aaacaggttc
    33541 gcttgacaaa acggggattg catccttcga atattcgtgc tactatttac ttgtttcgtg
    33601 ttactaatag aggaggtttc gccatgaaac gatcggtttc cttcgccgtt gtttttcttc
    33661 tcgctctctt ctttggcttc aacccgccgg cggccgcctc ggattcggac gcggttgaga
    33721 acgcccaaaa agacgaacgc ctcctccgcc cgcggcatga agtcacggtc acggcctccc
    33781 ccatctggaa ggccgtcaag gattgttccg tgtccgtcgg catcctggat gagacggaca
    33841 ttttagccgc ccggccgctc aacgccctcg gcgctctcac ccgcctcccc ggcatttttg
    33901 ctcaccggac gggcgatttc ggacggaccg acatcgaaat ccgggggatc ggccagcgag
    33961 gccagaggat cgccgtcctc gtggacggcc gcccggaaaa aatgggtttg ttcggctgcg
    34021 ccgtgaccca cgcctatccg cttgacaacg tcgagcggat cgaagtcgtc cgcggcccgt
    34081 cctcggtttt gtacggcccc gacgccatgg gcggcgtcat caacgtcatc acccgccggc
    34141 cggcgagcgg tttccggacg gaactcacca ccgccgtcgg cagctacaag accttctccc
    34201 tcaacctccg ccacggcgcc gcgtttgagc ggttcgacta ttatttcacc gccgaccgga
    34261 tgacgagcga cggacacacc gcccactctt cctacctggg gacggctttc accggccggg
    34321 cggcatacga tctcggatcg ggtctcaccc tgaccctgcg gggcaaggcg ttcgacggga
    34381 agaagaacga gcccggcccc ctggcctctc cgtccctgac atcctggaac cactacaagc
    34441 gcggttcagc cgacctcagc ctcaacggcc gctgggaggg aggcgaaggc tcgatcatgg
    34501 cctactccga cttcggccgg cacgtctttt ccgacggctg gagctcccgc gatcattatc
    34561 gcggagcgat cgctcggggc agcctggctc tcggagcgga ccatcgcctg tcgttcgggt
    34621 tggacgcccg gtttttcgga gggaaaagct acaacgcccc cagaggcgaa tgggaaaaaa
    34681 gcgacgtcgg cgtctacctt catgacgaaa tccttctcgg cggccggtgg attctttcgg
    34741 ccggagcccg cttggaccgc gattcccaat ttggatggga aggctcgccc caggccgggc
    34801 tcgtctggca tgccggggaa aatacgtccc tccgtctttc cgcttccaag gcgttccgcg
    34861 cgcctcatat caacgagctt tatcttttcc ccgcgtccaa tcccggtttg aagcccgagc
    34921 gctcttggaa tttcgagctt ggaatcagcc aagccgtggc ccggatcctc gcctttgacg
    34981 cggccgtctt caccatgaaa gggacgaatc tcatcgaatc gtccccgaat ccagccggtc
    35041 cgccgccgtt caagttccgg aacaccggac gcttcgcgtt ctccggaatc gagttgggct
    35101 tgaaagccga cgtcctccct tccctcatgc tccggggcgc atattcgcgg ctggacacgg
    35161 gcggcctgac gaaaggccgg cccgggagaa aaatcgacct tgagatccgc tggcaggccg
    35221 gccgcttccg gacgttcttg accgcccagt cggtttccga atactacgcc ggcgatcgca
    35281 aaacgctccc cattccttct tacaccctgg tcaacggccg gcttgaattc gccgtcactc
    35341 cctccgtcgg gctgttcgcg gagctcaaca acctctccgc ggcggcctgc tggatctacg
    35401 ccgacctttc cggaaaggcc gccgggttgt acgaaatgcc cgggaggaac gcccacgtcg
    35461 ggctcaaggt gggcttgtga gccggccccc tctcggtgga tttttcgacc gctgcgcgcc
    35521 ttcctgggat aacggctgtc cggccggctt gacggccgcg atttcaaaga tcttcgcggc
    35581 cgcccgcctt catccgggct cgcgcatcct cgatgtcggc gcggggacgg gcatcctcta
    35641 tccttatttc ctcaaagccg gagccggagc ttacacggcc gtcgacagct cgccggcgat
    35701 ggttcagcga ttcaaggcta agcacccgga ggccgccgtc ctcgaggccg attacgaaaa
    35761 gccgatcgtc tttccggagc cgttcgacgt catcatgatt ttcaacgcct tcccccattt
    35821 tcgggatgag gaagcggttt tccgccgctc ccgttcctat ctttcggccg ggggccggct
    35881 gttcatctgc cattccatga accgggaagc cctcaacgcc catcaccgcg acgccggcgg
    35941 gaccgtggcg ggcgacgtcc tgatttccga cgcgcggatg gccggccttt accggaaggc
    36001 cggattcacc gccgtccggg tcgaaaacac ggatcatttc tattccgagg gaacgaaatg
    36061 aagccggaat tgaaagacgt ttcaacggcc gcggtcttcg gggcggcggc cctggccctg
    36121 cccgttctgt ttcacgctct cgggctgggc tccgcttttc tcccgatgtt cctccctctc
    36181 tcggcggccg gatttctcct cccctaccgc gccgccggcc cgctggcggt gatcgtcccg
    36241 gccgcctcct tcgccatgac cggcatgccg cccatgacca tccccccgac cggcccgatc
    36301 atgatgctcg agctcgcttt cctcatggcc gccaatcgtt tcctccaccg tcggctgaaa
    36361 atcagcgtct ttcccgcagc cgcggccgcg gccgtcgccg agcgtctgtt ctacctcggc
    36421 ctcctttttc tcgcggcgtc cgttctccgc ctcccccgcc tggccttttc cctggccgcc
    36481 ctggtgaaaa gcctgccggg aacgattctt ctcttgaccg cggtcccggc ggtggtcaaa
    36541 ctcatccaaa agcacgcgga ggcatgatgc gggccgccct ccttcttctc tttcaagccg
    36601 cggccctgct ggccgacccg cgttttctcg ccccggctac ggtcgcggcc gcggcctggt
    36661 ttgcgggacg gaaggaaagc cgttcccgcg tcctgcttgg aacgggggtg ttcggcatgt
    36721 tttttttcgt cctttgtctc ctgggcgcgg tcgagggctt tctgaggctg tccgccccgg
    36781 attatggacg ctatgccctg ctgacgctcc ggggatacgc atcttttctc gtcgtcgtcg
    36841 ccgggacggc gatgttcacc ctggctgaaa tcctctgttt cctaaaaaga atccgcgtcc
    36901 ccggctatat tttgaccatg gtttatctca tggtccatga tttgtccgta ttcgtccgcc
    36961 tggcttcgga aaccggccgg gccgtccgga gccgcggggc gggcctccgt ctccctcagc
    37021 gcctcaagct cacgatccat gcggcgagga atttcatggt cttcgccgtc ttgaaattcc
    37081 ggctccggca tgaacacatc caggcccggg ggctgtccct ggaccttccc ctggatgaat
    37141 ggagggcgcg tgaacgcctg cctcaaagcg gatgacctgt ccttttggcg gggggaacgg
    37201 gctgtcgttt ccggcttgtc tttcaccgcc gccccgggcg accggatcgc tgttcttggg
    37261 gccaacggat cggggaagac gactctcatc cggacgattc tggggttttc tccttaccgg
    37321 gggacgctcg aggtcgaagg gcgggaggtc cgggaccggc ccaaggaggc ccggaaagcg
    37381 gccgcggccg tcttcggaga cgccgacacg cagctgctga tgcccacggt ctatgacgag
    37441 cttgcgtttt ccttgaaagc ggcggaagaa cgggccggcg ggccggatgt gcgggcgttc
    37501 gccggaagat tcgggctcgt tccgcttctc gaccgccatc cctcgcagtt gagcagcggg
    37561 gagaaacgga aggttctcct ggcccaaagc ctcggccgcc ggccgtcgat tctgcttctc
    37621 gacgagccga cgtcggactt ggacgcccgg ggatcgcgcc ggttgcggga gatgctggcc
    37681 caagtgccgc aaacgatcat tttagcgacg catcattacg acctggccat ggccctctgc
    37741 cggaaagccg tcgtcctcgg cggcggcaag gcggccgtgt atgaggacat ccggcgtctg
    37801 ttcgacgatc cagccgccct cgaccgctgg gagctcctct gagaccagtg aatctcattc
    37861 ggcccccgtt ttaattcagt acctattaat tcgacacctg tttatccatt tccgcaattc
    37921 aatcagtacc tgtttaacca tttccagaat tcgaatttgg gaaatggata accaggttca
    37981 gatttaaagt tcggattttg tcggaaatga ggaaatggtt aaacaggttc ggattcaatc
    38041 cgaacggctt gtctgtttcg cccgctcccg cttgatctcc gcttcccgtt cgagataact
    38101 ctggccggac gtgatccagt cctcggcggg ctcgcccttc agataatgcc cgaaccaggc
    38161 cagaatccgg cgttggtaat ccatctggtt cttctccttg cgcaacccat ggccctcgcc
    38221 catgtaagcc agcatcacca cgttcttgcc cgcccgccgg gcgacgttgt agagctcgat
    38281 gccctggtgc cagggaacga tcttgtcctc atcggcgacc tcgagcagaa gcgggacggt
    38341 catccggtgg gttccgaaat acggggagtt gtcgatgtaa gcctggaggt cctcatataa
    38401 aggaacgacc atccgctcct gacccgtctc gatgtgatcc gtctcggcga tgcccgtgtc
    38461 ccagtgatga tcgccgtaat agctgaccat gtcgatgatc ggcgccccgg cgaccgcggc
    38521 cgcgaaaatt ccgtcggtat gggtcgcgac gaaggcggtg ttgaagccgc ccatcgaatg
    38581 cccgacgatg ccgaccttcg ccggatcgac caggcccgtc tccacgactt ttttcacggc
    38641 cgcggtgacg cattcaacca cggaccagcc cggccggcgc tcgcgaaaaa cgatgtccgg
    38701 ctcgagaacg aaatacccct gggaggagaa gacgctggcg ttgtaatagc tccgttccga
    38761 cggagccgcg taacggtgga cgttctggga cagaagctcg tagttgtaga cgatcatcgg
    38821 atattgtttc cccggctcgt atccggcggg ataaatcagg gcggcctgca gcctccggcc
    38881 cttttccgtg acatactcga tgagctcgct ccgtccccag gcgaattttt cctggaacgg
    38941 attagtcgcg gtcactttga cggccccctt caaatcgggg ccgccgacgt ggatgtcggg
    39001 cgaaacgtca taatcctcga tggtatagcc gtagacttcc gccttttcag ccttggccag
    39061 tccgtcgacg gcccggtcga gccagatcaa gcgggtgatt ccgccgccgg ggcgaaggcg
    39121 ggcatatccg gattttttcg tccactctcc gaaaagctcc aagtaaaggg gcgcggtcaa
    39181 atccaccgcc tcggccggag gaggcggaaa tccgtatctc cgccgtccgc cgtcggccag
    39241 gtccaagcgg accaagcggt ggcggatctg ctcgccggcc ccgttcgtca ggcgcgccgc
    39301 ccccgagccg tccggggcga tccgccagag atcatacttg tcataaagaa ggacggcgcc
    39361 gtcttccttc ccccaaccgg cgacgccgaa cggcggcttg gccgggccgg tttcgtccga
    39421 ctccttatcg atgaatgaga cggggaggga agcggtgagg ttcacgaccg cccgggtggc
    39481 cgtgtcgacc gtccaataat gatcgtcctg gaggaacaga acgtacttcc cgccggggct
    39541 ggcctgcgcg tgcgcgtcgt caatgccttc ccggattttc gtccgggctc cggtcgcgat
    39601 atcggcgaga tagagatccg ccgccggccg tccgatcgtc cggttgaacg cgtatttcga
    39661 ccattcggcg acgagcgccg accgccccga ccgcagcggc gtcacctctt catggacggg
    39721 gtccaggccg agggggacga gcttcccggt tgaaagacgc agggcggcga gcgagtttcg
    39781 ccgacggccc gcgtcggcgt ggattttctg ccagggcatg acaaagacgt cctttgcgtg
    39841 ccagatttcg atggtcgaga cctcctcggc cgcggcggaa ccgccggccg gagctttcgt
    39901 ttcagccgga gcgacggcgt catcccaggg ggcgagtccc aggaaaaccg cgtccccgga
    39961 ggccgaccag gagagcggcc ggaagtcgac gatccgttgt ccggccggga aggcggcatc
    40021 cgcggccgag tcataaaccc agatccgttc gttcgctcca agccccgtcc aggccaagac
    40081 gacttgggcc gttccttcct tccggtcgtc cgttttggcc cgaaacacgg ccaggtccgg
    40141 cgcgtctttc cgccaggaca agccggcata agccgacgac gacgaatcca gaacgcggag
    40201 gacgccggag tccgggtcaa acaggtggac gccgttgccc gtcttgcctt tgacgctgac
    40261 gatcagagcc aggaaccggc ccttggggcc gtcctcccag agatattggg agacgttgcc
    40321 gaacggcgtg tccaggccgg tcgccagccg ccggacgagc agggtcgagc cgggagacgc
    40381 accgtcggtg tccggaccgc cggaccgatt cggggcgtcc gccgccgcct cgggaggata
    40441 cgttttcatc gccagggaag ccccgtcggg actgaaggcg aaggactcga tcccgtcgaa
    40501 cgtcgtcacg tcgcccgtgg ccaggttcag caacccgagc ttgtcttgga cggttttttt
    40561 ctcgtctttc agcttcttcg cgtccgcttc ggacaggccg acggcgaacg ccagccactt
    40621 cgaatcgctc gaatacgccg gccgggaacc aaaggcgaag gccttctccg ttttgtcgga
    40681 aatcttgacg atacggagtt cgttgttccg gttcgaacgg ttaaggccgt atacaagcca
    40741 ccgcccgtcc ggagagaatc cgccgtaggc gccggctgcg gcgatcgttt cccattggcc
    40801 gtaatcggtg aaggtcgccg gcggcttggc ggtcggcgcg ccgagcccga ggcccgcaaa
    40861 aagtgcgagg atgatgagag cggccggaac ggagcgtttc atcgcgcatc tccctgtccg
    40921 tgaggtggat tcccgaggga atcgaaatcc attttatcaa taaaagcgcg ggacgcaacg
    40981 atgatttctc catctttccg gacaagcccg ccgcggcgga ccggcgcctg ttaaatcgat
    41041 acctgtttaa ccatttccgc aattcaatcg gtgcctgttt aaccatttcc cgaattcgaa
    41101 ttcgggaaat ggttaaacag gttcggaatg gaatcagaaa tccagcttga agccgagata
    41161 gatctggccg tcgtcgcgga aagccccgaa cttcgtcttt tcccggtccc ccgagaccgc
    41221 ggcgaaggct ccgcagacga cgttgaaggc gtcggcgacg cggaattccg cctcgggcat
    41281 gatgacgaag gccgatccgt tggatgtggt ctcgaaaagc cctccgaatt taagcttaac
    41341 cgtgtcccgg gcgaatttat actccgcccg gcccaaaaca tagtttcccg gctccccgaa
    41401 gaacatcccc ttccggaagc cgaatgtatc ctccgctccg gcgttgtaat ccacctcgtc
    41461 gaaaaagccg tggaggaact ggatgttgac cgaaaagccg cccccgaaat tgtagtcggc
    41521 gccggccacg tacttccaga acccctcttc gaaaagcgga aaagtcttcg tataaatgac
    41581 cggcccgtct ccgatccaaa tcggaaagga aaccgagccg tcgatcttct ccggcatgac
    41641 ataggccgcc tccgcccagt atccaaggcc gccggcttct ccggccaaat ccaggcccag
    41701 aatccccgct cggggataat aaaacacgcc cgaggcgaca agcccggcat ccatcgaccg
    41761 cagataaggc agcgtcaagt tgccccgata atacgacacg ccgaggtcga ggccggccgc
    41821 ccgccccgcg acccgcgcac cccaggcggt cttgatcgcc gtgtcgtccc atccccgcgt
    41881 cacgagatat tcgtccatcg gaaagaactt ggccaccaag cccgaatatc cgaacgggag
    41941 cggagagacc tggtgctgga gcagtccgac cagctggatc ttgaacgaac cgcccgggta
    42001 atactccagg ttgacggccg tctgcggccg gcgctcgaag aaataatccg ggtcgaagga
    42061 caggaagttg gcgaagtcga tcggattgag gttgtcgacg acgccgatct tgtcggccgt
    42121 cccccaggaa atccgctgct tgccggcggt caagtcgagc ccttcgatca ggaagtcccc
    42181 gactttgacc cagccctcgt aaagcgcgag ctcgaaatgc tccgaggaag cgggcgatcc
    42241 gaggaaggcc gactccggga actggccggc ttggagaagg tcgcccgggt aggagaacgc
    42301 gtcgaaccgc acgccgtagc tgacccggtc gttgaccgcc ccggagagct tcagccggac
    42361 ctcggcccgg cggaaggcga actggccggc cttgtgggtg aaaaacgctc cgtccgggtt
    42421 gtcggccgcg taaaaagagg aaaacagccg gacccgaccg gtgatttcgg gcgccgccgg
    42481 ccccgtcgtc tcggcggcga aagcgaggct cgccgccaac aaacccacgg acaggaacat
    42541 cgcggttttt ttcacggctc cctcctcagc cctggatctt ccggaccagg ctgcgctcgg
    42601 tgaagaggtc ggcgccgacg cccttgtcga ctttcatgtc cttgaactcc agggtcgtgc
    42661 tcgagccctt cttgtgatcg gtcatcttga cggcggtcat gacccagtag ccgtcgatcg
    42721 aggcgtaggc ttgctcggcc gacttccaga gacggccggc ctcgtcgtac atttccgtct
    42781 tgaccggcat atgcatccgc ttgtccacgg tcatgacgat ccgggaatac aatttatccg
    42841 acccggcttt ccgcttgaga tccagcaccc aggcgcccgc gttctcttcc tggagagccg
    42901 gttcataaga ccgggcgtat tcgcccgtcc ccaggtcgtc ataggagaaa tccgagctca
    42961 tgaacgaatc cttccggttg gacgaggcga tccgccgggt ccggtgaaat tcgggcaggt
    43021 agatgtacat cgagtcgtcg ctcaggacga ggaagccgac gtcgcggacg tcggccggcg
    43081 aaacgaattt catcagccgg tcgtcgttct ttccggcgtt gttccgcgtc caagcccgga
    43141 tctcccggac cttgaccgag ctcccctggg tgatggtcat caccatgttc gactgcatgt
    43201 ccttcggggc gagggtgccg ccggtccgtt cgtccacccc ggccaggacg gccgcggcgt
    43261 cctccgcggc ggcggccggc gcggaggcgg cgatcagcag aagaaccgaa gcgaggatga
    43321 gagacgtgcg tttcatatcg aatctccttt cattatttcg ccggcggggc ggagcacccg
    43381 gggccggaag accaggaggg cggccggcag cagggtcatc gccccgatga agcagagaag
    43441 catcgacagg accatgatcc cgccgaagct tcggagcagg acaaaggaag acaggagcag
    43501 aaccgaaaag ccggcggcca cggccacggt gttggagagc atcgcccggc cgcgctcgat
    43561 gaaagccagg cggatggcct cgggcaaggc atggccggcc cggatctcct cggtgacgac
    43621 atagaggaaa tgaatcgtgt agtcgatgcc cacgccgatc gaaatcgacc ccgtgagcat
    43681 cgtcgcgtag tcgaggggaa tgcggacgaa gcccagaagc ccgtacatca tgaccgtggc
    43741 gaaggtgatc ggcaggatgc tgatgaatcc caggacgacg ctcctccgca tgaggatcat
    43801 caggaggagg gtgacgatca tggccaggat gatcgactgg atctgggagc gcaggagaga
    43861 gtccgccagc ttggtggtca tgacggggta tcccgtctgg tcgacccgga taaagtcgac
    43921 cggctcggat ccggctggag cgtccgccgc cgggacgacg gccaggccgt cgaccaggtc
    43981 gtaaaggatg gccgccgccc gcttttcaaa ggacggagac ccggcgggaa gaagggcggc
    44041 cagcgcctcg cgcagcgacg cttcctggag gctctcccgg gtttctcgga cgaggtaggc
    44101 ggccgaggcg gccgcgtcgg ccgccagatc cggatcgaac tcggccggct tgaggtgttt
    44161 cttgaagacc gcggccgccg cctcttcggc gatctcgaag tccggcgaag cggccgccag
    44221 gcccgacagc gcccgggata tcgactcgag ctcccgggga gccgcctcga agggaaagga
    44281 gggcgcgaaa caaaagtcac ggatcttttc gacgacggcc ggccccaggc gatcggcggg
    44341 caggggcttg ttcagcgcgg cgacgaggag atcacggatc gggccggccc cgggagccgg
    44401 gccgcccgat tccccggccg cccaggctat ttcctgcgcc aggaaggccg cctcggcggc
    44461 ccgaacggcg gccgccccgc tcggggacag cgtttttaga tccacccgga cggggcgcat
    44521 agcctcttcg tcttcgaaaa aggattcggc cagggcgaac gtctcctcgc aaaagggagt
    44581 ggtggaatcg ctgaccttgg cgtagatgat cgtctggcgt ccgtcgggcg tgatcatctg
    44641 ggtcagctcc ttccggcctt ccaggaaaag ccagaggttc tcgacggccg ccttcgtttc
    44701 cggcaaggcg ggacggtcgt tcatcgagtc attgagctcc tcgatgagat cggggatccc
    44761 gaggggctgg tggacgcttt ggaggcccga aagatagctt tcggcccgcc ggataagacg
    44821 cagccgggcc gggtcgcgga cgtcgtcggc ctcgaagaag aacgaatcgg gataggcgcc
    44881 cttgaaatgc tcgttggccg cgtccatcgc ccggtagggc atggattttt tcgcgtagaa
    44941 cccggcgaag ccgacttccc gctcgacgcg gaaaatgccg gcgatgccga aggcgatgag
    45001 aatgccgaac gcggccaagg cgaggcgggg ccggcggatg atccggccgg cggcggactc
    45061 caggaagccg gagagccgga aacgggattt ggagggacgg acatcgccga aatcgatcgt
    45121 cgcggccagg gcaggaagaa gggtcacggc caggccggcg gcaaaaaaca atccaatggc
    45181 cgcgaacaat ccgaaatcgg cgaccaggct cagcttgacg ccgcggaacg acaggaatcc
    45241 gatgatgtcg gtgaccgccg catacaggat cggggtaacg acggccgcgg tcgagacgat
    45301 gaccctttcg cggcgctgct tcacatcgat attccgcccg gtctcgacgt cggagcggat
    45361 cttgttcacg acgtaaagcc cgtaagccga tcccaaggcc aggagaagga cgggggcggc
    45421 cggggtgatg atcgtcatcg gccggccgga ccagccgatc agcccgaaca gccagatgtt
    45481 ggccaagacg acgaccagga ccggcggaag gagggctttc cgggaacgga gggtgaaata
    45541 aaggatgagg acgatggccg ccagcatgat ggggacgaga aagacgaggt cccgccgggt
    45601 gtagagattg atgaaatggc cgtcggaggg cgtgccggcg aaatagatct ccatccctcc
    45661 gccgaggatg cgctccgtct ccgggatgag gaccttttcc acggcgtgga tggtttcgac
    45721 ggtgttgctg agatagacgg acaagccgaa ccattctccg tcctccgaca agacctggcc
    45781 gacgtagcgg tccttggacc gggccagcgc ttccagggct ttcaagccgg ccgaatcgac
    45841 cgggacggaa ttgataaaat cccggacctc gatcccgtcc tccgttttgc ggatatcggc
    45901 gacgttggaa agggaggtga cgctgaaaat ctcctcgcgg tccttcaggg tgtcggtcag
    45961 ctcccggatg cgggcgagcg tgtcggccgt gaagactccg gctttcggac gggcgatgac
    46021 caggacgatg ttgttggccg agaacgtgtc ggagatctcg tggtagaggg ccaagtgcgg
    46081 gtcgtccttg gggagccagt cggtgagctt gttgtagaag cggagacgcg gaaccccggc
    46141 cagggcggcg agggtgagca gcccgcaaag gcccaggaca agaacccggt ggcggacgac
    46201 gaaggcggtc catttcttag ccacgggaca cctccgcggc cggaaccgga aggctcaggg
    46261 accggagata atcctcgata taggggcggg ccgtttccat gtagctccga gcccgctcct
    46321 caaagaccgt ccaaagggtg aacgtcgtgt agatgacgcc caggaaattg atcgcttgga
    46381 tttcgggatc ggcgtcggcc cggaaatcgc ccgaagccac gcctgtctcg atgtttttcc
    46441 gcaaccggcc cttaaggtcg agcatgcagt ccttgaactt cttgaagagg gggcggtctt
    46501 cgacgtacaa ggcgtcggaa aagaggatcc ggtggatgcc cgggtattgt tcaaggaagg
    46561 aaacgacgaa ttcaaaccaa gcacggaggg cggccggcgc agcggaggtc cggctggaga
    46621 tttcccgatc caccgactcg atcgacctga aggaggcgtc gagaatgaga tggaaaagac
    46681 ccatcttgga atcgacgtgc ttgtagaggg ctccctcgac gaagccgacc tccttggcga
    46741 tccgcttgat ggtcaggccg gtgattccga ccttgtccac gaggaggagg acggccgtca
    46801 cgatttgggc cttccgtttc tccaggatgc cctcgaaagc aagctttttc atgttgatta
    46861 ctccggtgag tgaacgttta ctcactttgt aatttatacg gtggaaaacg ttttgtcaag
    46921 gcgattatcg ctgttcaaat caccgctgaa tcggcgaatt ttcccaagga atcggtacct
    46981 gtttaacctt ttccctgttt ccccctctat tcgaataccg aacctgttta tccatttccc
    47041 tattttttcc cctgatcagc cgaaatgcgg aaatggtgaa acaggtactg atttaatccg
    47101 aggaaatggt gaaacaggta ccgatttcgg aacaggaact gatttcagtc tcggattttg
    47161 aaaaagcggc cggcgagaat cgaaagcacg accgccaggg cgagtaaaac gcccaggttc
    47221 ggagccacgg ccgcgaaatc gccctggaaa aaccggagct tgtgcaaggc gtccatcgcc
    47281 caatacgccg gcgagatcat gcccatcgtt cgcacggccg gcgggacaac ctcgatcggc
    47341 caccagcagc cgcccaaacc ggcgaagaca ttggccagca ggatcgaaag cccgatgatc
    47401 atatcctctt tccccatgag cgagccgata aacacgctga gggcggccgt cgccaaggcg
    47461 aaaacggcga tgaccagcag cgacaacggc acgtcgccca ggttcaagcg gaaaaagatc
    47521 ttgccgacga cgaagagcat caacgcctgg agcattccca tcagccaccg ggcgaggagc
    47581 ttggcctggt aaagcgcgcc gggcgaaagg ggagaaaaga gcagccggct caacacgccc
    47641 ttcttgcggt cctccatcac cgtgacgccg ccgtaaatca agaccatcat catgatgaac
    47701 tgaacggtga tgccgggaat cgcatggtcc atccccccgg ggacaacggt gatcgtcttc
    47761 gccggatacc gggtcttgac cgtgacgagg tcccggaatt ccgcgtggcc gtctaaaaat
    47821 ttcgtcaaat ccccgtcccc gtagagcacg agctcgctta gaagccgggc gatcgcctgg
    47881 tacagcctga tttcgacccg ggcggcggcc gcggccgagg cgtcttcccg tttttttaaa
    47941 gccagggcct ggtccttgcg agcctcgatt ttttccgaga aatccggcgg aaggatgatc
    48001 agccggttgt attcggccgg ttcggtcccg gccgttttca acacgattcc cgggctcttg
    48061 attttttcca ggaaatacgc cccccagcgc ccctcgtctt ggttgaggac ggtcagctcg
    48121 gccgacggcg gagcggtttc acccccctta tacaaataac cgaaaagaag gatgaagagg
    48181 atcgggaagg ccagggtcca gaagaaaaag accttgtcct tgaccatgat ccggatgtcg
    48241 ttgccggcga tgcggagaat gcgtttaagg cgtcgcatgg gtcacaccgt ggagcggcgg
    48301 cggagcagcg gaaccgccag gaaaagcaaa ataagaccgc tggcggccag gaccaggagg
    48361 gccgccgccg gaaagcgccc ctcccggacg agagccgccc cgtcgatgaa ccaccggttg
    48421 ggagtggcga gcccgatcgc ttggaacgcc ttgggcatcg cctccgtgtt caacatcgag
    48481 ccgccgaaga cgctgaaagc caggatgacg ggggcgctga tcgccccggc ctggtttcga
    48541 ttcttgagaa aggcgttcat cagggcgaaa aaggaagccg tccagaaggc cgtcaccgcg
    48601 gcaagcagga gaaaataacc gggggatccc caatacgcct tgaacaccag cgttccgaag
    48661 acggccatga ccaggaggat ggcgaatccc atcagccagc cgccggcgat gcgggccccg
    48721 atgagctcac ccggccggag gggcgtcgtc agcatccggc ggagcttccc gtcctcgcgc
    48781 tcggaaatca tgtcgcgcat gaccgtttgg acgatgaaca gaagaaacat aacggccatc
    48841 cccggcagga cggcggaaaa aatatccgac cgggtcatga acgccttggc ccccgcggcc
    48901 ttggtttttt cctcctggag acggatcaga agcggattga ggtatttttc agcggcgacg
    48961 accttttttt gggctttgcc gaactccggc cccaggctct cccagggaaa agcgtcgatc
    49021 gggccgtcga gcatgagacg gatgccccgg gcctcgggct cgaaggcctg gatcgcgccc
    49081 gagagcatca cggccatcgt gttcatgaac tcctcgacga cgtcgggaag aaactgctcg
    49141 gccggatttt tgaccacctc gaggacgacg ggtttgattt ccaaaacatc gagggtgaac
    49201 cgcgcgggaa tgacgaccat ggccgaggcc ttgcccttct ccatccgccg gcggccctcg
    49261 gcctcgtcgg ccacggtcac ttggaacatt tttttcatct ggtcggcgtc gaaggcgccc
    49321 agaaaaagac gcgaggccaa gcccttgtcc ttgtcgacaa gaacgacctg gatcggcgga
    49381 agcgtgttgt ccttggagga tggggtgaag atcatcccga tgaggccggt catggcgatc
    49441 gggatgataa gatagaggag gatcgccacc ggagccttaa ggtggcgcaa aatatcattc
    49501 ttgagaatca gcagcagccg gcgcatgact caatccctca ggctgcggcc ggtcagcttt
    49561 aaaaaaaccg tctccaggct gggcgttttg atcttgagat cggtcacggc cagcttgcgg
    49621 cggaagaggc ggtccatggt cttcggaatc tgttcggtgt cggcgacggc cagcgtggcc
    49681 aggttgtccg cggccgacag caactcgatc ccgttgccgt caagatcctg gagggcttcg
    49741 gtgaacgagg cggccgggaa atttcccgtg atctcgacaa tgtccttttc cccgacaagc
    49801 cggatgagct cctccatcgt tccctgggcc aggattcggc cacaatccat gatagcgatc
    49861 cgggaacagg tgttttccac ctcggccagc tgatgggtgg tgaagatgac gccggtaccc
    49921 tcggcgccca ccttgcggat aaggtcaaga atgctgactt tggcctggac atcgatgccg
    49981 accgtcggct cgtccagcag cagcaagtcg ggccggtgga gcaagccgat ggccgtgttg
    50041 aggcggcgct tcatcccgcc ggagaagttc ttgaccggtt ccttcatccg gcccgagagc
    50101 tcgaccctct cgaggatttc ggcggcccgc tttttcagcg cgtcgcgcgg aacgtcgtag
    50161 agtcctcccc aaaagaggag gttgtccatg gcgttcagct cctcgtagaa agccatgtcc
    50221 tggggaacga tcccgaggcg gtttttcagg ttccggtcgg aaaagagatc gtggccgagg
    50281 aagcggacgc tcccggtgaa atcgcggacg gtgccggcca ggatggaaat cagcgtggtt
    50341 tttccggcgc cgttggggcc gagcaagccc aggacctctc ccctttcgag ggtcagggaa
    50401 aaatgatcta gggccaggag gtcgccgtag gacttggtca agttcttgat ttcaagcata
    50461 ttccactttt tcgtgaacgc atcataccat agaatacgcc ccggaagcat gaacggttga
    50521 tgggattttt agccggtttt aatcggtacc tgtttatcca tttccctgtt tttcccctga
    50581 tcagccgaaa tgcggaaatg gttaaacagg taccgattga gagattgaga tatcgatcga
    50641 tcttagaaaa gaaggccgag caggacgatc aggacgacgg ccgctaaaag aaggatcctt
    50701 accgccggga tgcgcataac agctcttcga tcgccttgaa aatcactttc gaatcggctc
    50761 ctgatcggat accgatggac tagcgatggg gcgcgggtcg tccgtcccgg gcttcccgtc
    50821 ccccgcccgg ccggctgaaa ccggccggga cggggacgcc gtcgcctcgg atgaaccgga
    50881 aatcccgact ccgcccacga cggtcactcg cagaagacgc ggaccttgtc ctcgccgtcg
    50941 acttcgacgg tcatgtcatc aagattggac acgatttctt ccaagtcttc aggcgtgatc
    51001 ttggacaggt cgacattcat ccctttttcc ttgagagcct catcaatctg gccctggtat
    51061 tcccggggaa tgaaggccgc gaacttgagc cccgctcgga tcagcttgaa cggaatccgg
    51121 atgttgacct tgtccttgct ttcgcttccc ggaccgggat caaccacgac ccggaggtac
    51181 ttccattgac gtttcccgcc cgtcgaggac acggcctcgt tgtccggctc gccgatcgcg
    51241 gccaggagct tctcggcttc atcgaccgtg atttttccct gggacagcat ctccagcact
    51301 ttgcgttttt cctcgctcat cgaaattctc cttttcgttt ccgtgatatc aggcaaagtc
    51361 gatgagaacg tcgttctcgt cgtctttcgc ttcgatatga aggcccgaca ggttccagag
    51421 aacggccgcc agcatcgggt agaggaagag caggaaccgc cccggaccgt tccgccaggt
    51481 gatcagggcg gccaaaagca ccagaggaaa gagggcgatc aataaagccc ccaggatgat
    51541 ccagatcagg atcaccggga accagaggcg gaagccccgg cggccgggct tgcacactct
    51601 gaggcgaagc cagaaaggca gcatgaattc ctcctttatt cctttatccg tttgagcgcc
    51661 tcggttacgt cgatctcgcc tcgatcgagc tgatcgagga cgtcccgttt ggccgagggc
    51721 ggattgacgt cgacgaaggg cagctgggcg gcgatcgcgt tcaagcggtt cttgaccgtc
    51781 ggatagctaa ttccaaactg ggcctccatc tccttgatcg agccgtggct ccggacgaac
    51841 gcgatgatga acaggacatc ctcggcccgg agccgagcca gagggggcag ctcgaagctt
    51901 ccctcgatgg cgatgtccct gtttttgagc cggacctttt cgaccgtgaa ggagacgccc
    51961 tgggtcaagc tgatcaattc gctccagtct tgagtcatgt cttcctcatt aaattcattt
    52021 tacatagaaa taataaaata tttattaata tatgtcaagt gttttattaa ttattttaat
    52081 aattatatta attttcttaa tattaactat tatttatatt aaattcaatg gtttatctgt
    52141 tcccacggca tgaaacaagg gccgtgtccc ccctttcgcg ataattccaa tcggaacctg
    52201 gtttaccatt tcccgatttt tcctgatcat ctcggtacct ggtcatccat ttccctgttc
    52261 tttcgcctga tccggcggaa tgaggaaatg gataaacagg ttcagattcg cgcggattcg
    52321 cagaagcggt tgtattaaat cgaattatgg aaatggataa acaggtactg attttactga
    52381 tttaacaggt accgattttc agaactccgt ccgtcggaaa gcgtataata ggataagcct
    52441 gtcgcttttc agggctgtga ttcctatttg gagagacaga caaatgcaaa gagcttcgtt
    52501 caaattcgtt ctcacccttc tggccatgat cctcatcatc tccgccgcgg ccgtccccct
    52561 gcccgcggcc gtccttccct cccccactcc tcaagccgag ccttcggccg agccgaccct
    52621 ggccggccat tgggaaggca tcatcgagct tcccgggcaa aagctcgaga tcaatatcga
    52681 tttcgtcctc aaggagggag cctggacggg cgatatctcc atccccgtgc aaaaatccca
    52741 ggacctcccc ctgatcggaa tcgtcttgga cggattcaag gcggcgttcg ccatccaggg
    52801 cattcccggc gagccgacct ttaagggcga aatctccgcc gacggcaaaa aaatgacagg
    52861 gacgttcacc cagggcggag cgaaattccc cttctccctc gagctcggcg ccgatccgac
    52921 ggctaagatc cgggccgccc tggaaggatt cgatcaaatc gccgaggacg ccctcaaggc
    52981 gctcaaagtc accggcgcgg ccatggctgt cgtcaagaac gacaaggtcg tttacgccaa
    53041 ggggttcgga tacaaggacg tggagaacaa gatcccggcc acgcccgaca cgatcttcgc
    53101 catcggttct tcctccaagg ctttcaccgt cttcgccctc ggccggctgg tcgacgaggg
    53161 caagcttgag tgggaagcgc ccgtccggaa ctacatcccc tggttcaagc tgtacgacaa
    53221 ggaagccggc gcgcgtctct cggtccgtga cctggtgacc catcgctccg gcctcccccg
    53281 ccacgacctc ctctggtata acaataaaac cgcgtcccgg gaagatctcg tccgggcctt
    53341 agcctatctc ccgcccacgg ccgacctgcg cgagaaatgg caatacaaca acctcatgtt
    53401 tctgacggcc ggctacctcc tcgaaacctt gaccggcaag ccgtgggaag acgccgtccg
    53461 gagtctcgtc ttcgaaccgc tgggcatgaa gcggtcgaat ttctccgtcc tcgattcgca
    53521 gaaggacagc gactttgctt ttccctacaa cttcgtcgac aaggcgtacg agaagctgcc
    53581 tttccgcgat atcaccaacg tcggcccggc cgggtcgatc aactcctcgg tgaatgaaat
    53641 ggcccgctgg gtggccgtcc acctggccga cggaaaattc gaaggccgtg atcttctctc
    53701 ctcctccacc gtatccgata tgcacaatcc ccacatggtc accggggcgg tctccagctc
    53761 ccccgacatc gccgccacgg cgtacgccat gggctggttc acagacgtct atcgcggcca
    53821 ccgccgagtc catcacggcg gcaacatcga cggcttttcc gcccaggttg ccttccttcc
    53881 cgacgacggg ctgggctttg tcgtcctgac gaacatgaac ggcacgtctt atcccgaact
    53941 tctcgtccga acggcgaccg ataagatcct gggccttgag gcccacgact ggatcggcga
    54001 cgcggtcaag caattggaaa aggccgaggg cgcaatcaag aaggccgagg aaaagaaaat
    54061 cacccgcaag gtccccggca cgaaacccgc ccatcccctg gccgactacg ccggtcttta
    54121 cgtccatccc ggctacggtt cgctggcggt cgagctcgcg gccggccggc tcagcttcac
    54181 ctacaactcc atcaagacga acctcgacca ctggcattac gagaccttcg acggcgtcac
    54241 gatcgaggat ccgacgttca aagacttcaa gctgaccttt aggacggacg tcaacggccg
    54301 ggtggcctcg gtcaccgggg cgttcgagcc gacgatcgac gatatcgtct tcgccaaaaa
    54361 acccgacgcc cgccaatacg atccggcttt tcttaaaaaa ctcaccgggc gctacgactt
    54421 gatggggcag acggtggtca tcggtcttag aggggaccgg ttgaccgtgt ccatcgccgg
    54481 ccagccggag taccagctgg aaccggacct gggcggcgaa ttctacctca aggaagtgaa
    54541 aagcgtcacc ctccgcttcc tcaccgatgc gaagggcgag gtcaccgcgg ccgaattgag
    54601 ccagcccggc ggcgtgttcg aagccaagaa actcgaatag gatccccgcc cgggaggcct
    54661 cccgccgccg ctgtacaacg aggcggcggg aggcttccgc tttttcctcc ctttccctcc
    54721 gccgccttac ctggaaaccg gccgccttcg ccccgctccg gtttcagacg gagaaatttc
    54781 catccctttt cctttgtccc cccctcctcc cgccgaaaaa aagaaaatcg attctcgagg
    54841 gtcccgccgg cgccctcccc gcggttattc ccgcggttct tatttatccg ttcttttcgc
    54901 cggatatgtt ccgtcgacga tgaacccggt gatccggccg cccgcgtcct tgaggaaaag
    54961 aaggctgagg ctgagatcca tggcgaaaaa cgacatctcc gactccggat agatctccct
    55021 caacgaatcc gcggccgtgg cggccagatg gtcgtccttg acggcgatgg tcacgggcgt
    55081 tttttgcccc tcgaaatcga tctcgtagtt tccggcgaac cgctcgagag ccgccgcatc
    55141 catcgggacg atctccttct cgatcacggg ccgaaacgcg ggcaggccgt attcagcggc
    55201 gatcgcccgc tcgatctcgg cgatcaacgc cgagccgttt tcggagttgg tcatgacggc
    55261 catccccgct ccccgctcgg ggaagaagac gagatcgcag aggaatcccg cgttgccacc
    55321 ggaatggctg aagcggacgt ccgcgcccat gccgtcgagc atgaagccca gcccgctggg
    55381 cgccttttcg attgtcaaca tggcctgggc cgttttttgg cttaggacct tgtcggaccg
    55441 gccgtcgcgg gcagccgcga tctcgagggc gaacttgcag agatcggcgg gcgtggtcca
    55501 cagcccggcc gcggccagct cgggatagac gtgacggcgg ccgccgaccg gcgtcccatc
    55561 ggatgtgtag ccggcggccg cccgggaaag gagcctatcc ggaagcggct gggcgtaggc
    55621 gctttccgtc atccccgccg gatcgaggac gatctccttc atgatgtcgg gaaacggccg
    55681 gccgataaca tcgaccaaca gctgctgcag cacggtgaag ccgccgcccg aataccgcca
    55741 ccggaaaccg ggcaggagat tgacgcggat gggcgccgag ttggccggcg gttcgccgtc
    55801 gaggatctgg cgcaacgccg ggagcggttc acccgccgga taaccggcga aaccgtgaac
    55861 cgtcagcccg gcgctgtggc tgagaatccg gcgcaaggtg accttttgtt ttgccgtgaa
    55921 gctgttttcc ggcaccttcc aggacgaaag cttcgcgtta acgtcctcgt cgaggccgag
    55981 cacgccgcgg tcgacgaaat gaagggcggc cgctgcggcg accggcttgc tgatcgaggc
    56041 cgcctgaaag agcgtttccg gcgtggccgg taatttttct tcgacatccc ggaagccgta
    56101 cgcccgggtc cattcgatcc ggctgtcccg ggtgacggcg atgctcacgg ccgggatttt
    56161 cagggcggcc attcgggcgg ccacggttga cgtcacgacg ggccggccct tgaggtgaag
    56221 ggagggagtc aggttgctca aaatccgttc gattttcgca tccgaggcct ccgccgccgg
    56281 agcgttttgc cctcgtgccg ctgaaggcag tccctgtctt aaattcagcg gattcggtac
    56341 cgggagggaa ttcggtacct gtttatccat ttcctcatta gcgggaattt gggaaatggt
    56401 taaacaggta ccgattcctg agcgggcgcc gattcctcgg ccggacgccg ccgtcgcaaa
    56461 tgccagaccg ataattccgg ccgagccggc aaggacaacc atgatgatcc aggcggtctt
    56521 tcttctgtgc atgatcgctc cctttttcac ctcattccct cgttcggcga attcatgata
    56581 ggctcaacgg caaaaccgag tcaaactcct gtaccggatc acgacatggg ggacgcctcc
    56641 cggggcttgg gggattaagt tttccggcga cacggttcca cggggggtca gaaagcccgg
    56701 ggcctgatgg ggccgcttcg actcgtcctc caaggtcgtc agatcatagg gatcgaaccg
    56761 ccccggttat ttatggaaag ttgtcgggga gatattagag cgacgacagg ccagaattat
    56821 gtttccgctc cgacggtgga agtcgatcca ctttaaccgc ccggcggagg ttttagaggc
    56881 atccaccatg tatctgaccc ggatcacgcc cgggacggaa atcgggaaat ggttaaacag
    56941 gtaccgatat gaatggggaa atggttaaac aggtttcgat ttgatcaagg ccggccggcg
    57001 atcatcgacc aagcgaaatt cagaaagaaa tatccgaaaa aaaggatgtt cagcaccaaa
    57061 accgcgatcg accaaccccc ggcccgggcc ggccgcggca gcttcgtgtt gaggtagatc
    57121 aacaggaccg gatagatgag cgaggccagg gtcgacatat tgccggcgat ctggaggagc
    57181 cgggccggca gcgccaagtg gatgatgacc gcgatgacca ggatgaacaa gaccgccatc
    57241 ccgtaatagg cccgcctcgg atcgccgttg atccgctccc tcaaccgcgg gctcacggcg
    57301 atggccgaat ccgtcacgtt ccggatcaag atctccagaa ccgacgtctg ggtcttgaaa
    57361 agaatcatcg ccccgatgat cagcacgaac acaaagaaaa acccgccccg ccttcccagc
    57421 tccacggccg cgtacaccgg gatgttctcc gtcgtcggtt cggccgctcc cggcgcggcc
    57481 gccagggccc gcaccagcac gccgggaacg aacatcccga tcatcgcccc ggtgaaaaaa
    57541 acgccccact gatcgatctt caggtaacgg aaccagcgct tccagagggc ggcgttttcc
    57601 gggctctccc ggaacgtcat cccggacggg cgaacagatt ttttttcgcc gccgacgaga
    57661 ccggagaaaa atccgacctt gtggcccatg ccatagccgt gatcgcggta gtaattgatg
    57721 agcagaaagt tcatccccga accgaatccc gtgtagccga tgatcgatcc cagcagaacc
    57781 gggtcgatgc ccgacggagg ggccgccggc gtcacgattc cccttaacat ctcgacccac
    57841 aaggctcccg gggcgaaaac aacggccagg atgacgaccg cccccagtgt caggatcacc
    57901 ataaacgtgt cgaccaaggc caacgtccgc tcgatcttcc gtccaaccga gtaaatcagc
    57961 agcgacagga acatcaggcc gaccgcgatc aagcggtagg cctcgacttc ccccgggcgg
    58021 gaagcgtcgg ccgcccgccc cgagaacagg gcgaagaggc tctggccggc ggcggccgcc
    58081 cagccgcccc agatccaacc gaggatgatg atgaacaggg tcgccgggat ccagagcttg
    58141 aacccgggcg gcacccggct gaagccgacg atcggaacct ccccggtggc gatgacatag
    58201 cgggcgttct ccacgttgta gaggacttgg agaatgcctg aaatcacgat gagccagccg
    58261 aggcccaaga atccgtattt cccgaaggcc agcggcccga gcagccattc ccccgagccc
    58321 agcgacgcgc ccagggcgat catgctggga cccaggacga cggtgacgat ttcccggaag
    58381 ccgatcctcg gcttgtcgaa gacctcctcc ggagtcggca ggtcgtccag cgtcagcggc
    58441 ggccggctga cgccgatctg gagagagtcc ggtttgcgat caggcccagg ctcgggcccg
    58501 gccgccggag cggaagacgc gtcgccgccg gcgctggtga cgaaatcagc catgaatcaa
    58561 tcctttccct tcaccggccc cgggacggcg gacgcaagcg aagcggttgt tctcttcctc
    58621 gccggttcgg ctcatgatta acccgaagct tcccttcgga gtcaaggtcg gccgaatccg
    58681 gcggggcccg gccgaggcga tgtcctcggc ccgcttcccg cctccgggcg aagccgctct
    58741 ccccgcccgg aaacgacgcg ccgggacaac cgcggcgccg ccgttttccg aaaagggaga
    58801 ggaattgagc cgccgaggac gcaaagcggc gggcccccgg acaaaatagt ctccgggggc
    58861 gcggcggccg cggtctttcg cataggcagg gaagaatata tgccggccgg cgaagtgtca
    58921 acacgcgcgc ggacaggcgg cgccggcttt atttcttttc cccgccgagg agggcgtatt
    58981 cggccatgat tttccggaga gtcgccttca cttgcagatc cagggggcgg ccggcccggt
    59041 agtcggccgg agccgtaaaa ttcacccggt tctccccccg gagccgctcc gcgaccgctt
    59101 tctttccggg cccgggcggg aagacggcga tcgagtatcc ccgctgctcc ttgaccagct
    59161 tcatgcaagg gatgtccgtc gtcccgtcgc cgatgtagat catccgggag aaaggaaccg
    59221 gccgctcggc catcgggatg aaatcgttga tccgggagtc atcccaggga tccaaaacgc
    59281 ccttgttgat ccggaacaaa aattgcgtct tcgtcgtgaa gttgagggcc agggccggcc
    59341 aataagccac gccgttctgg tcgaacatga acgacgaggc gtagatccgc tcaaactccc
    59401 cggcgatggg cgtccccagg atcatttccc ggatgcccga ggaaatgatg aaatgccgga
    59461 cagaggcgcc caagcggctt ccgaaggcgt tcatccgggg aaaccagctt ttcacgccgg
    59521 ggaagagggg gatggtccgg ccgtagtcga tgaaagcttt cttcgtgatt tggacgccgc
    59581 tggccggggt ggctttttcc agcatcaagc gcatgtagga gtggatctcg tcggcgccct
    59641 gttcccgggc ccgttcctgc gcctcccgcc agaatttgcg gctgctcagc cggagcttgg
    59701 ggatgtagtc gtactcctgc atgttgcgcg gggacagggt tccgtcgaag tcatagcaga
    59761 cggcgatgat gggcgggtcc tggcggggac gggggcgggc ttgagcggcc gggcgattct
    59821 tcatgttttt ctcgctcttt gggttccatc gttattttat acgagggaag aagataaatc
    59881 atccggtcgc gcgcgttcag ccgcggcctc cgcgcgccga acgccggccg cgaaacggag
    59941 aaagggaggg cgacgggccg ccggattaac cggccgatcg gcgggcggat ctagattttc
    60001 gaaaggatga aggccgtcag gaacccggac atcgtcatga aagcggtgag aaagccgccg
    60061 ttttcatagg cctcgggcat catgccgtcg gcgagcatgg ccagaatggc cccggaagcg
    60121 aacgattcga tgatgccgag ggcgttggga tccagatggt gaagaaacag gaagctcaaa
    60181 acgacggcgg cggccacgca gacccccacg atcgaccagg acagggtgat ccgccgcccg
    60241 gagaagccct ctttccggag atcgtcgatc gaggaaaggc tttccggcag gttggagaga
    60301 aagaccgcgg ccagcatcag aaacccgatt cccttcctgt cgagaagggc gacgcccagg
    60361 gcgaccgatt ccggaatgcc gtcgaggaga gagccgagga tgatggcttt cccgtttgtc
    60421 tcccgcgccg ccggaacacg taatttaggc cggtggcttc ttcccccgag gcgatgaatc
    60481 agcagatccc cggcgatgaa gaccagcccg ccggataaaa acccgatgat gatcgcgtcg
    60541 aacccgccgt gcttgaaggc ctcctccatc agcccgaacg tcaaggcgca gatcaagacg
    60601 cccgagccga aggccatgac ggccgcgacc gcgcgctggc tgattttcag gctggtcccg
    60661 atgagggatc cgaggatcaa ggaaaaaccg gtaagggcgc cgaaggcgac gaccgttgcg
    60721 agggtgaaca ccgccgccaa tatagggccg cggaacgggg aagtcaaaca gggaacgctt
    60781 ccccttccct gaaaaggggc gaggcgcccc cgaatattaa atcgagtatt taaagagctt
    60841 catcccgtcc agcggcggaa cgacgccctt gtaaatgttc cagtagtatg ccttggcctc
    60901 ggccagcttg gcgtagaggg cgtgcctttc gtcgggcgcg tgggtcgcga cgattttttg
    60961 atcgccgtcg accaggagga gcttggcgca gcggttgagg atgccggccg ttccgatgct
    61021 ggccacggtc gccaattcct tcgcccggac ccggcggaga agctcgccgt acgacagatg
    61081 cctctcctcg accttgacgc ccaggtgggt caggatcttg cagattccct ggatggtgac
    61141 ggagggaaga atcagcgggc tttccgggat cttgacgacc gtgccgtcgg tgaaggcgaa
    61201 caggcagcag gacgaatccc actcggtgat gtgccgttcc tcgacgggcc ggtgcgtctg
    61261 gtcgtccagg aaaagagccg cggcggccga cgggtcgatc gtcttggcgg cctcgacggc
    61321 cttgaccgac atgacgtagt tgacacccag cttgaggcag ccggtgccgt tcagtccgac
    61381 ggcccggacc aagtgcggga tcacgacgcc ggtgaacggc tcgcccagat actggtcgta
    61441 gcgggaagcc agggcccgga tggccggctg gttggggaac gtgacgccga tggactgctc
    61501 ctcgtcgacc gtgaacggcc ggaggtagcc ctgggcgccc atcgcggcca tttcctcgaa
    61561 aagcccgcgc atcgccggct cgcggaagaa gcggtcggcc aggatatcct ccagctcgtc
    61621 ggccgtggga acgagcggag cggagtcctt gccgatgttg aagagcatcg agcggcggaa
    61681 ccgctcgagg ttcttgtccc aattcaggaa tacgacttcc aatcccccgg aggcgttctt
    61741 gcggcagaaa aagcggatgc cctcgaaagc caggaagatc gcgtagttgg tcgagcggga
    61801 gcaggcggcg attttcgcgt cgctctcgag cagctcatag gatttatcgg ccaagccgta
    61861 acgcatctgc ttggacgacc atttgagacc ttcgaatttg gccatgtcga ctctccttgg
    61921 cgccgggacc gggacaccga cggcgtcctc agatccggga agcgcgatgg tttttcgcta
    61981 aaagaagagt atttggtaac acatcgaacg gcccctgtca aaccggccgg gacgatctcc
    62041 ggctcccctc cccggcctct tccggcctat ccttttgggc cttccttccg ggaacaggcc
    62101 gcggcccgtc aaaggcggga cgccggcgtc cggggggacg cggcccctca aaaaaggggg
    62161 gcgccccctc aagctccaag ccgccggaga gagcgaggga ggcgcctccg ctttcctctt
    62221 ttttgatttc atccgccatt tagactaaaa ttcggaacgc atgatcgctt acctgaaagg
    62281 cgtcatccgc caaaagtccc cgtctcaaat cgtcgtcgat tgcgggggcg tcggatacgc
    62341 cgcggccatc cccctgtcca cctttttcaa gatgggggaa gagggcgaac ccgtcgagct
    62401 gttcatccac acccacctca cggacagcgc cttgtcgctt ttcggcttcc ggacgcggga
    62461 ggagaaagag ctctttctca aactcatctc catctccggc atcgggccga agctggccat
    62521 cgccgtcctt tcggggatgg acacggaaac cctggccgag gccgtgcggg ccagcgacgt
    62581 cgcccggatc gcgatggtcc cgggaatcgg caagaaaacg gccctccgga tcgccatgga
    62641 gctggccgac aagatcgaaa gcaaggaaaa agtcctcaag gcgcgcgccc caaaggaaac
    62701 cgaggatctt gtctcggccc tgctcaacat gggcttccgg cgcaaggaaa tcgagccggt
    62761 cgtcgagaat gtcgtcaagt cgggcaagaa cggcgatttc gaacatcgtc ttcgggcctg
    62821 cctgaaggcg ctttccaagg tctgatatgc cgcctctcgc cccctccgcc gtcctcaacc
    62881 ccgtccggac caaggaagac ctcctgttcg aggactccct ccggccccgg agcttcgacg
    62941 agttcatcgg ccaagacaac atcaaggcca acctcaaaat ctatatccag gcggccaaga
    63001 agcgaggcga acacctcgac cacgttctgc tgtacggacc gccgggattg ggcaagacga
    63061 gcctcgccta cctgctgtcg cgggagctgg gcgtcgggtt gaagccgacg gccggtccgg
    63121 tgatcgagcg ggccggggac ctctcggcca tcctctccaa catgcagagc aaggaagttc
    63181 tgttcatcga cgagatccac cgcctccacc ccagcgtgga ggagatcctc tactcggcca
    63241 tggaggattt cagcctggac atcgtcatcg gccagggccc ggcggcccga agccataagc
    63301 tcatgctcaa gaagttcacc ctggtcgggg cgacgacccg ggccggcaag atcaccgcgc
    63361 cgctccgcgg ccggttcggg atcatccacc ggctcgatta ttacaaggac gccgatatca
    63421 aaaagatcat cctgcgggcg gcggatctgc ttaaagtcaa gatcgacgac acgggcgccg
    63481 aacaaatcgc caagcgggcc cggggaacgc cgcgcgtggc caaccggctg ctccggcggg
    63541 ttcgggacca cgtcgacgtc aagggcaagg gcatgatcac ggcggccgcg gccgatgacg
    63601 ccctcgacct gatggaggtc gactcgctcg gattagacga cgtcgaccgg aagatcctca
    63661 cgacgatcat cgagaacttc ggcggcggcc cggtcggaat ctcgacgatc gccgcggcca
    63721 tcgacgagga aaaagacacc atcgagtcca tctacgagcc ctttctgatg cgcatcggat
    63781 tcctggagcg gacgatccgc ggacgaaaac tgacccggaa ggcctacgac catttcggac
    63841 tcccccaccc cgggcgcggc cgggcggcgg gcgcggccgg agggacggcc cagaaaaagc
    63901 tgttccgcga ctgaaggacg ccggcttccg ggacgcgcgc ttaaatccat gctggtcaaa
    63961 gaatacgatt tccccctgcc ccccgagctc atcgcccaga agccgctggc ccgccgcgac
    64021 gattcgcgga tgatggtcgt ccggcggaag gaagggacgt tcgcccacgc ggccttccgc
    64081 gatttcccgg attatttcgc ggccggagac ctgctggtca tcaatgacac caaggtcatc
    64141 ccggcgaaaa tctggggccg gcggggaggt tcgctcatcg agttcctgct gttcaagcag
    64201 cgcgagccgg ggacctggga ggttttatgc cggccggccc ggaaaatcag gccgggcgat
    64261 cgggttgatt tcccgggcgg gctgtcggcc gaggtgatcg aaacgggcga tgaggggcgc
    64321 cgggtgctta aattcgccgt gccggacgtc ctcgggcgcc tgcgggacat cggattcgcc
    64381 ccccttcctc cttacatcaa gaggccgaaa aacgacgacg ggggaagggc ggaggattta
    64441 aaccgctacc aaacaacgta cgccgagaac gagggggcga tcgccgcccc gacggccggg
    64501 ctgcatttca cgccggatac gatggagcgg ctgaaagcgc ggggcgtcga ggcggcccgg
    64561 gtgacgctgc aggtcgggct ggccacgttc cagccgctgc gggtcgagag ggtcgaggac
    64621 caccggatgc tggaggagat ctacaccgtc ccggaggagg cggtcgcggc cgtcgagcgg
    64681 gcgaaagccg ggggccggcc ggttacggcc gttgggacga ccgtcgtccg gacgctggaa
    64741 agcgcggcca gggcggggga agcggggatg acgggggacg cggcgccggc ggcgagggag
    64801 gccgggccgg cggcggaagc gggagaaccg ggcgggagga atcccgcggc cgaaatattg
    64861 atcgcgccgg ggacgcgagg gacgtccctt ttcatctatc ccggcttcga gttcaaggtc
    64921 gtcgaccggc ttctgacgaa ctttcacctg ccgcagtcga ccttgctgat gctgatctcg
    64981 gccttcgccg gccgcgagct gatcaaggcg gcctacgagg aagccgtccg gaaaaagtac
    65041 cgcttcttct cttacggcga ttgcatgctc atcctttaaa tctgaacctg gttaaccatt
    65101 tcctaatttc cgtttccatg gaaataagga aaaggttgaa caggcactga ttttaaacgg
    65161 acgccgattt caaaagcccg gatagatccc cgatccctca tccctgggaa aagcagaaaa
    65221 tccgaaaacc ggcttttcgc tgagcttcaa ggcgaaatca tccggggaaa gaacaacgcg
    65281 tcccccgatt gatcagccgg tgacgatgcc caagggagga ttgatcttcc acttgccccg
    65341 ggtgaaatcc gggatctcga acgggcggct gccggccgcc acgctcccgc agctggctcc
    65401 gacgaccgcg ctccaggcgg ccgcgtcgta gacgtcctgg tcgagaggct cgcccttgag
    65461 gagggactgg atcaggcggt aatcctcgat gtaatccatg ccgccgtggc ctcgtccttc
    65521 ccccttggcc gcgatcgcct tccagagagg atgttcgtac tcggccgcga acttctcgaa
    65581 gtcgtcccac gcatgggatt ccgcggccag cccctcgaca tagacgcgat cgggccattt
    65641 ctgggcgacg cctttcgtcc cctgaacgag attgatccgg gtgtagggcc gggggaggtt
    65701 ggtatcgtgg atgaggatga tcgtcttgcc gagcttggtc cggatgagag tggagttgac
    65761 atcgccgagg gcgtattttt cggcggcttg gggcgaggac ggcccgaaat tcgcggcggc
    65821 gtactcgtgg agcccgcggg ccggagagct catcgagacg atgaagtcga gggcgtcgcc
    65881 gcggttgatg ttgaggcact gggcgaccgg cccgagacca tgggtcgggt agaggttgcc
    65941 gttgagcttc cgggaccagg cccgccgcca aagcccctcc ccctcgctgg agaacttgac
    66001 ctcgcggagg tcgtggaggt agcccgcctc ggcatggagg atttcgccga ggacgccgcg
    66061 acggacgagg ttgagggtca tgagctcgat ccggtcgtaa cagcaattct ccatcatctg
    66121 gcaatgccgt cccgtttttt ccgccgtctc gaccagggcc caacagtcgt ccaaggtcat
    66181 ggccgccgga acctcggtcg cggcatgctt gccgttcctc atggccgaaa ggcagaccgg
    66241 gacatgccac tcccagggcg tggccgtgaa gaccaggtcg agatcttccg tctcgcacat
    66301 ccgctcgaag tcatggtctc cccgcgtata cgccgagggg aggggctggc cggcctcgac
    66361 gacccatttt tgggcccgct cgaccttggc tggaacgatg tcgcaaaggg ccttgatttg
    66421 aacgccgtcg atgttgagga gattctgaac gtgggccgag cccatgcccc ccacgccgac
    66481 gaagccgatg cggacgcggg gaagcggagc ggcggcgaaa gcggtccggc cttcgcccgg
    66541 aacccggcgc tgatcgccga gaagaggctt ga
//
LOCUS       DAOOXH010000004        60660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_405_length_60660_cov_7.712449, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000004 DAOOXH010000000
VERSION     DAOOXH010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 60660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60660
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_405_length_60660_cov_7.712449"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PKV64_01025"
     CDS             complement(<1..496)
                     /locus_tag="PKV64_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018035007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPN15689.1"
                     /translation="MPRSTKKKSKFVGMRLSEKQNEALSRLQKDSKASKSDVLLRGLE
                     LLSEYYSLDLDKPPLSFDLRSLETEAMHLAESLKQVRDKKQAIHQMVQELAAVDRILD
                     KYDGRENALIQVLLDIQTENHWLPKPALIWASERLNVPLSRIYHIATFYKAFSLEPQG
                     RHAVQ"
     gene            complement(672..1175)
                     /locus_tag="PKV64_01030"
     CDS             complement(672..1175)
                     /locus_tag="PKV64_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15690.1"
                     /translation="MKQEAMPEQVKSNRSGRPPKFREPRRPVTMTLPVRILDLLAEID
                     PDRAQAVAKAAEAVAGAEADRFKPVKLTEMAQGKSLIVVGPSKALKEIPWLKLIEIAP
                     TRFLLSIPSGTPVEALEVALRDLTNDPVLRNNDRETAILAELLSLIGHQRRMNRLGKA
                     EFLIIDA"
     gene            1425..2603
                     /locus_tag="PKV64_01035"
     CDS             1425..2603
                     /locus_tag="PKV64_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15691.1"
                     /translation="MKKLSGQIAPALFAVGLIFFNAGISMSQTTSVLKEMSLQKGGAR
                     LNFLFKVEGAYVVEASFLPSPPRLIVDMTPISEMQILPVTQIGDMGVLDVRAVQSGPD
                     KIQIIFDFGPMIPAYGITQGAEGLKISFWNEGEIAAISDPRPAEAAKPIVSTAPAAPV
                     APVQARSAALERSGFFIAGRAGLSLFVGEDLSVEKTFDLYGETATLAESYSFRTVPAF
                     ELQFGRYFNRTKIGIGIGYSPLKSPGSFTAGLPHPFLPETPRNVSFESDGGKNTLWDF
                     SVFALFSFLNTEMLSVAAGPMIGLSLGAMQSMEDFDFTEESPYDAAHVTVANMTYAKD
                     QFTELSFGMLLSLEYRLNEGLGLLFDLRGVYLNPKNVTLGKRANLLHLQPVLGIQYSF
                     "
     gene            complement(2677..4350)
                     /locus_tag="PKV64_01040"
     CDS             complement(2677..4350)
                     /locus_tag="PKV64_01040"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPN15692.1"
                     /translation="MKKHIMLGICFLLLLTNLQFGQILGNGRLLFQSPEKIRWTQCAF
                     TPDGRLWVVWVPGTTNANSGGPIWVGTYDGTTFSTPINMTGSLAIKANRPHITTSSKG
                     HVLVAWGVVATRSTYLRIWDPKTKIWGPIETVASNYGGDEPIAQMDNAGNIHVFFTDE
                     AGGVVFARSKINGVWDNIAILNQSRGKQGSLVIGPDGKVHAVWIEKDPDSTYQNYYAT
                     RTISTAWETRVALPKTPTNSNHPWISIGSNNAPIVCYQDVPPAENGCEIYVLPIGGTP
                     VRVIPFAMQHFGRVVVDSKNDIHVVCQTGGGDFGSGFRYTNNVGGIWKPFQTFPALYP
                     KVHGLAADSLGNVAVTQSSYTTSGTDLWVYSLNPIERVDMPTADFTFSPASGYPPLAV
                     DIQAVPAFSPNGSEVSYAWTFSDGGSAAGRQTSHVFETHGMHTITLKITDNLGRMDEI
                     GKTIEVKKTDPQIPLNAELTIVIGSVRRNLSITYSLAWRINPANTPAHIKGYAIYMKE
                     GDGEYAPILTVSPSTFDSSFTFTDLKVKRYFAVSTLGYGGTESAKMYFQ"
     gene            5183..7132
                     /locus_tag="PKV64_01045"
     CDS             5183..7132
                     /locus_tag="PKV64_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPN15693.1"
                     /translation="MMQKKVLIVDYDMKDTTRVSELLTNYGFEVAIAGDGQAGWDQYS
                     ALKPDVVLLEPMLSKIHGFELCQRIRSDSLGRTQVIIVTGTYKDPIYKTETLRTYGIF
                     RYFVKPVDNDKLLAAVNGAFNVGKEHEPGQPEFIPPVAASSKPPEEKLRPVAEKKEFD
                     FEQPEFIPPVPASFKPPAEKPRPVEEKKEPESKQPEFIPPVPASFKPPAEKPRPVTEK
                     KEPESKQPEFIPPVPASSKPSLEKLRPAAEKPKQRTPDAGNVEVDALLKKALKGFDLE
                     TGLIKSPGKEPKKAPAPEPPRRPVPAPEIRKTEPIAPKKEERPPKPVPPAAPPPRTES
                     SVHTERTPFGDLYEEPAKKNFKPVVIIAAVVVLAVAAFLILKPGSPGATNEVQAGGSS
                     LLEPAADLETGSMLDSETVAIGSEIQETDPENAVKPEPTKPVDKIETARPRPSEQPAA
                     PPAAQAKPVARRPESAPEAVRSVEPEATKVQRPAETVGDKRESTPAPAASTPAEKPVQ
                     ESAPPPEVKESVPPAAAQAVTPPPAEVKQPPAVKPSPPSVQRGQLVPLNEVSQKPSVV
                     RRVMPVYPEIARRLKIEGSVTVNALIDESGNVIDTEVIKGIKNDSGTHRAAEAAVRKW
                     RFNPALVNGVPVKVWMPVALVFKVE"
     gene            7166..7402
                     /locus_tag="PKV64_01050"
     CDS             7166..7402
                     /locus_tag="PKV64_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15694.1"
                     /translation="MTKDQEALYRRTLDDVKKQLDQLDEEVEIELQKVRLRLAELQES
                     KKSLKKVYEGIANLLGIEMEKEADEDQAAAISKV"
     gene            7679..8482
                     /locus_tag="PKV64_01055"
     CDS             7679..8482
                     /locus_tag="PKV64_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPN15695.1"
                     /translation="MLSFSFAGRDALPESRHFTLEKPAEGVYAAIHNDLGGFAICKAG
                     NIDLGDRTVILDPFICPTAARDLKRQAEFLTGRPVSLVLNLDPHSDPIGGNQVFIPGA
                     VIISTANARAYIETHFQKDLDTQKETVPGELKIVQDRLIRASGIERFELALRESEYKA
                     LVDAFPEIEMTLPDMTIEGPMTIHGSKRNIVLVPVGRGHTTGDMIVSLPGDRIVFMSD
                     ELFVKRHPYLGDGDPESLLSSLKRILALNARPSPCRDTVPSAGRIRCER"
     gene            8479..8700
                     /locus_tag="PKV64_01060"
     CDS             8479..8700
                     /locus_tag="PKV64_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15696.1"
                     /translation="MIDYIETLTTLVQEEIRMGAGENDVPRLPVSEKFKDWLIEGFYV
                     VNLRFLYRSMIPRPDPKRTRLPSPGKGAE"
     gene            8799..9056
                     /locus_tag="PKV64_01065"
     CDS             8799..9056
                     /locus_tag="PKV64_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15697.1"
                     /translation="MMPEEAIGKVTHYFDRIGVAVLQLTGTVRVGDTLHFQGHSTDFN
                     QAITSLQIDHKNVSEGNPGQDVALKVDRKVHPHDHVLKVTA"
     gene            complement(9236..10096)
                     /locus_tag="PKV64_01070"
     CDS             complement(9236..10096)
                     /locus_tag="PKV64_01070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN15698.1"
                     /translation="MSPIPAVTIREMNPGDIEFAAALARAEGWSSESPDAFAAFLESD
                     AAGCFIAEAGGERAGVCVAAAYGRNGFIGELIVSPRLRILGLGRQLFEKALGRLDAAG
                     LENILLDGDLNAVPFYEAQGFRKICRSLRFRGRIAGKKHDHIRRLDPEDLEPLCALDR
                     ELFGDDRGFFLRRRAGRFPDLSLVSERGGRLSGWIMARPGGGLLAIGPWAALEPEDAG
                     PLLEHLASEHKDEIFRVGVLEANAAAARRLRSWPGFEEGVFSWRMVRGPSGRLGDHPA
                     LYAIGSAAKG"
     gene            complement(10173..10655)
                     /locus_tag="PKV64_01075"
     CDS             complement(10173..10655)
                     /locus_tag="PKV64_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HPN15699.1"
                     /translation="MSAALLIVLGGVLQAAGLAGCVLPVMAGPPLNFAGLILLDWARG
                     WNSFSPAFLVVMGLLTAGSMILDYVLPLSGTKRYGSSKRGFWGAFIGMAAGVFAFPPL
                     GIIIGAFLGAVIGELAAGKESPAALRAGWGAFIGFLLAMGFRLALSGVMTFAFVLKLL
                     "
     gene            11169..11846
                     /locus_tag="PKV64_01080"
     CDS             11169..11846
                     /locus_tag="PKV64_01080"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPN15700.1"
                     /translation="MNDESKPRIPLIGEKAPAFKAVTTQGEIHFPEDYKGKWVILFSH
                     PADFTPVCTTEFMTFAKMEEEFKALNCSLIGLSIDSHFSHIAWLRTIKEKIAWKDMRN
                     IEVKFPVIADLTMEVAKSFGMVQPAASNTQAVRAVFFIDPAGVIRAMIYYPLSNGRNF
                     DEIKRLLVAMQTSDKHQVATPADWRPGDKVIIPPPGSCGQAKDRVEGAGSAYECLDWF
                     LCFKKLP"
     gene            11993..13456
                     /locus_tag="PKV64_01085"
     CDS             11993..13456
                     /locus_tag="PKV64_01085"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPN15701.1"
                     /translation="MLLQSVFFLYYFCLVFCLVWLTPKSLRWLPLLAASFFFVAYGHP
                     GHLLFLVLPTLVAYAAARRLAALPPEARKARKRWLWAALILILGSLVAVKYVNFFMGT
                     AAGVVSLFGSGWTWKPLKMILPIGISFYTFRLIGYLIDVYFKRIPAETHAGRFALFAS
                     FFPQVSAGPIERAGRFLPLLRHPETFSAERIFSGASQAAWGFFKKMVIADRLAVFVDP
                     VFADPSGQGLNLVFAAWLYAFQIYCDFSGYTDIAIGLARMLGYESAPNFNRPYLSVGI
                     ADFWSRWHISLSTWLRDYLFLPVSYAVMRPLKQDRLFGVKVESWAYITSALLTMTLCG
                     LWHGPTWAFVFWGALHGAFMIAGQLGRKRRRRWARKAGLTSRPALLRALRVILTFNLI
                     SFAWIFFRAPTLSKAFAYLHFLQLKINPSGWSFLVVGVLLILVLLGGERLAASDKSSG
                     WWSRRPLPLRGAALALFVCLIILFAVDTGNEFIYIQF"
     gene            13431..14435
                     /locus_tag="PKV64_01090"
     CDS             13431..14435
                     /locus_tag="PKV64_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15702.1"
                     /translation="MSSSTSSSERLRFLALLACFLAALLVFDRGLSLAVRAIEHRSYG
                     KSDFEQRLGRYLAGRNPAVLVFGTSRTFEGIIPAHLESCLGKKIFKEAYWGKGPKYNY
                     YFYQLYKKRVGVPKLVIYGVDYFIYNAESNVRWMSRFNLGEKAAGFDVLAGPLLLFKN
                     KKHNDEFLNNLMTDLGRTAPAADSGGDEDLFDKINTHPGAPAPDDNPKLRTERPPQFF
                     KQRFPKPPGKEGDYFLRLLDELKADGVKVALVSLPDYIGSLRTNWQMPGFHRHLQDLS
                     RRYDNVTLLIYNTPRDFDLDKPDLFLDGGYGKTNSHLSQRGAAVLGRKLCRDLAPLSR
                     "
     gene            14567..14881
                     /locus_tag="PKV64_01095"
     CDS             14567..14881
                     /locus_tag="PKV64_01095"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPN15703.1"
                     /translation="MNQEKRRSPRGARMTEIVYTHEGLHFEGRLSDLSEGGLYIDTIN
                     PVPEGSIIAFRFVLVEKDGDSIIEGEGRVAWQRPFQGMGIAFTRLSDDVRDRLKAHFS
                     RA"
     gene            15259..16947
                     /locus_tag="PKV64_01100"
     CDS             15259..16947
                     /locus_tag="PKV64_01100"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPN15704.1"
                     /translation="MKRSFLLSIGAVLFLATVSFGLTLGHPKLISKTTDRVRWTQCAF
                     GPDGVLWVVWVPGDTNRGSGGPVWVASYDGTVVRGPDNVTESTAVLANRPHISVSPKG
                     SVIVSWGIDADKSIHLRIRDVQGSWGAIETVSTGLGGDEPAAFMDKAGNIHVVFSDEV
                     GGSVYALSKINGAWENAVKLSTGFGQHGSLALGTDGAAHAVWVERSGSSGNNYENYYA
                     KRTASTSWSTGEALTGQSGSAAHPWVTVGTNHVPVVAWEDLPDPDKPGGGSEIRVMMV
                     GETARLAIAVGLNHFPRVVVDRNGAAHVACQSGRGDAGNGIRYTNDASGTFQAVQTIA
                     GVYPKLAGLAADPYGNVAATQSSYLTSGGTDIWAYSLNLIAAVPAPIAAFTFSPSTGY
                     PPLAVTFQAAAAFGPNGEEVNYDWVFGDGGTASGRNVNHVYQASGTYNVRLTVADSIG
                     RTDEEIKQIVVLRTVPLPPVNLSASIAMTRFWENPEITFNLYWAANPDNIAEHVDGYV
                     LYMRENDGSYNRLLTVSSSTFSVSFAFTNLQVSRSFAVSTLGKGGTESAPAYFQ"
     gene            16974..17627
                     /locus_tag="PKV64_01105"
     CDS             16974..17627
                     /locus_tag="PKV64_01105"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPN15705.1"
                     /translation="MTRISKTAGAGGAAAGKPLRVQLGCFDRSADGWLNTDITPHIRI
                     TRVFGLPWLLFRFGLIDRLRLDQHRASVFSRVKKLDLRKTFPFADGAVEAFFCSHVLE
                     HLYLFQTRAVLDEVRRCLKPGGFARFVLPDLDAAVRLYRKENPDDFLQVLFQRDSPAA
                     EKNRHKWMYTAPSFIALLREHGFDDVRERGYRDSGYPPFLPLDNRPEDSFYVEAVKD"
     gene            17640..18611
                     /locus_tag="PKV64_01110"
     CDS             17640..18611
                     /locus_tag="PKV64_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007203899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPN15706.1"
                     /translation="MPLPLRRLGRSDLLVSPIGLGCWQFSQRRGWNKYWPKLPEREIE
                     GIVAASLDGGVNWFDTAEAYGDGASEEALSRVLQKAGKKPGEIIIATKWMPLLRTAAG
                     LPRTIGKRLECLAPYPIDLYQIHHPGSFSSTRKQMEAMASLVREKKIRCAGVSNFGAK
                     SMRAAHRFLAEAGLPLVSNQVSCGLLDRAIEKNGVLDAARELGITIIAYSPLSQGVLS
                     GRFHDKPERLQEISGMRRYRGYYRKNALRRSRPVIDALKEIGARHGATPARTALAWLV
                     RFYGDTVVAIPGATRASQAASNAAAMAFDLTSDELDHLDRVSRDFSF"
     gene            complement(18608..20044)
                     /locus_tag="PKV64_01115"
     CDS             complement(18608..20044)
                     /locus_tag="PKV64_01115"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPN15707.1"
                     /translation="MTFLSYSFGLFFLASAAVYHAAPRKIKPGILLAASLVFYGWARP
                     VFLILLAGEALAAFGFALWLEKKDRPGRRIVFVTALAALLAPLLVFKYSDFLLRAFRP
                     GEAGALLGLILPAGISFYTFKALSYVIDVGRGRISAERNPVLVGAYITFFPQILAGPI
                     ERAAPLLAQLRGDYRFDLGRAAGGFRLLLWGLFKKIVVADRMAQYVNIVFETPADYAG
                     LGLVIGLVFYSYQIYCDFSGYSDMAVGLGRMLGFETMDNFRFPYFSRSIAEFWSRWHI
                     SLSSWLRDYLFLPVSYALSRKIKAGRVLFLKTEFVLYLAAMSVTMLLCGLWHGANWTF
                     IVWGAVHALYLVASRATQGVRKRTARRLRLRRSGRAWGFFRTVFSFSLVTLAWVFFRS
                     PTLAAAGNYLSHISWALPRRGVGVILFLGSILAVFILAEVLIMNRKALWGDRRVPAPL
                     QAAAYALFLCLIIILAADTSNEFLYFQF"
     gene            complement(20041..21045)
                     /locus_tag="PKV64_01120"
     CDS             complement(20041..21045)
                     /locus_tag="PKV64_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15708.1"
                     /translation="MAFGTLVFAVSFTLSDRLLLRGLRSWANTYYASRDADASLRNKT
                     VIYGEGNGDILIFGSSRARFAFGQFQMSDRLKARTIKVAAAGRFPRFSRLFYLRHRKE
                     NAAKPRAVFYGIDYFMFEKKSMLNELAALDRELRADALNPAEAVNEASSLLSRISLLF
                     RKKPDIDDFFGAVIKLDRARDVAGEDGADAVDVLNAGERTDRPAPRRPYRPQPGAEGA
                     DLRKLLAVLEEDGVPAYLVILPDIASTNAINFEQDKYKSDIRTLAGSYKNVTVLDFNR
                     PDRFDLNDAALFRATKGPRSNCHLSEEGRIRFTRNLVEAVLPFFARRGAEEIPPRSRS
                     "
     gene            complement(21161..23320)
                     /locus_tag="PKV64_01125"
     CDS             complement(21161..23320)
                     /locus_tag="PKV64_01125"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPN15709.1"
                     /translation="MNTRLRKLITAVALTAVFLPVFLSALTTLELATTAKPPVIDGKL
                     DPGEWDGAYAMSEFKTFQPDYGKEPSQKTEAYFLYDADNLYFAFRCYDTEPSKIKATL
                     AKRDDMYADDFVGIVVDTYNTMQNGYSFLVNPLGVQGDGIMTTDGNLTAEQDFVWDSK
                     GRLDDQGYIVEYRVPLQSIRFPSGKTITIRLAYFRQIVRTSEMASAPPVYPDKSSFLA
                     QTQPIAVTGWRFKRVVEILPAVTHSDRRAVDEGTMKREERATDVSLTAKVGLTTDLTL
                     DAALNPDFSQVESDAGQVDINLRYALFYPEKRPFFLEGNEIFSVAGNTEDAPLYALLH
                     TRTIIDPTYGFKLSGKIGAANSIAAIFARDNQPGADDEHPYFSVFRLKHALKDDSFIG
                     GYYTARDVTGGFNRVIGADGRFRLSPISFADFHLFGSFSKAPDGGETAGGHALGLRYN
                     QGTRNYYLDFGYQDISRNFQVDTGFLERTGLRRLAACGILMFYPKSKIIQRYDTFYWS
                     YHLQDTTSNMFETFNMFCLRFFLPRSTQVRFEGMLANEVYEARRFGRSGYGFQFYSQL
                     TKQVYLNLFYRNRGSIYYDPDDPYQGDSNQASAGLSFQPTDQLTFALDLVYRDFARRS
                     DKVKIYDYTIVRSHNTFQINKYLFLRAIAEYNTYYKRWTADFLASFTYIPGTVVYAGY
                     GSAFERIRWDGLEYVESERYMETKRGFFFKVSYLWRW"
     gene            23477..26134
                     /locus_tag="PKV64_01130"
     CDS             23477..26134
                     /locus_tag="PKV64_01130"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPN15710.1"
                     /translation="MNNRFFRIGAALLILGAVGQVLAGAAGGPGVKKCAAIRIGESVP
                     VIDGRLVDSAWENGSWESGFVQRDPYEGREPTEQTSFKILYDDKNVYVGIRAHDTRPD
                     LIEKRLGRRDQAGGDVVLVAFDSLHDHLTAYVFSVNAAGVKADQIVANNGLSAGGGEE
                     DMSWDPIWDAAVSSDDQGWSAEMRIPLSQLRFGAKDEQEWGLQVLRTLFRTNETSEWQ
                     LIPRKAPGWVHLFGELGGLAGLAAPHQVEVMPYAVGSLMSSRPVPGNPFATGRNRRLR
                     SGLDGKVGVTSDLTLNLTINPDFGQVEADPSVVNLTALETFLQEKRPFFIEGRNLFDF
                     RLTSGDGDFSYDNLFYSRRVGRSPQVMPGGPGFVDMPAAATILGAFKLTGKTKSGFSL
                     GLMESVTSRESASVFHDEVYTDVAVEPLTNYFAARVQKDWNSGATVLGGMITAVNREA
                     AGEAFETLHRAAYSGGIDFSHSWGNRNYYLSFKAVTSHVRGTAEAILATQLSPLRNFQ
                     RPDAGYVTLDPRRTSLTGTGGNFEIGKQGGGNWTYAAGVTWRSPGLELNDAGYLRQTD
                     MIMAFVWAGYRVTEPFGPFRRVNVNFNGWAGFNFGGETIFEGGNVNLNLTLKNEWEAG
                     FGYNPQGPSLSAAALRGGPTLYLPGGNHFWGYVGTDSRRKVRFSLFGSGGRRGPAEIV
                     SWSMSPELTVIPSSAFQVSLAPSYSRNDNVLQYAGTRTAGGEPRYVFGTISQETLALT
                     LRMTYCLTPDLTIQYYGMPFVSAGAYRDFKTILNSRSRIPEERFRLLGEAAVYDPSAR
                     QYRVDENGDGTVDYAFGNPDFNVREFRSNLVLRWEYTPGSALYVVWSQGRTGFLPEGS
                     FDYGRDLGGLFDTHPDNVFLIKFSYCFQL"
     gene            complement(26175..29270)
                     /locus_tag="PKV64_01135"
     CDS             complement(26175..29270)
                     /locus_tag="PKV64_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPN15711.1"
                     /translation="MTISEFSIRRPVFTVMITLVIVVVGFVALNRLPVDLMPEITYPT
                     VNVVCSYENTGPEEIEELITRPIEEAMSAVPGVEEVFSTSSEGSSVVRVTFDWGTDLD
                     AAADDIRERLDRVVGRLPEDVDRPTLRKFDPAQFPILMVGVLSNLDPIRTRRLIDEQI
                     VYRLERVPGVASVDVWGGREREIQVNLQADKIKALGIPMDTITSVIRRENLDLPIGQI
                     ERGNYEILLRIPGAFTDLDQIRNTVVAFRNGSPILLRDIASIEDTFRKITRVVRINDK
                     PGTRIAINKQSGKNTVAVAEGIKAELAKIREDLPQIELIVVSDSAQYIRRAINNVGSS
                     ALYGGMLAVFVLLFFLGNIRSTLVIGAAIPISIIGTFALIYFGGFTLNIMTLGGLALG
                     VGMMVDNGIVVLENIYRLHEGGLPIRQASVLGSQEVISAIIASTLTTVSVFMPMVFIR
                     GMSGIMFKQLAYVISFSLIVSLFVALTLVPMLCSRYLRVEKIEPGSAPGFHVAFVRST
                     RGVVAKLEKAYRRLLVYALDHKAVILGIAGGLLIVSLLLIRAVGNEFMPATDEGEVRV
                     SVEMEVGTRLGLMDDKIKQVSAVVRRTVPEMISLEENAGGGGWRSGSNTGNLTVKLVP
                     KNKRSRSSETVAADLRRDLSDIPGVIIRTRATGGQQGMMMRGGGSMARLQIEIRGYDL
                     LVAEDLARQVQKKVEALPGVTDTRISRDLGNPEELFIVDRKAAADQKLSITQIGQTIQ
                     TILMGTQATTFREGGREYRVLVKVKDSEFLSIDEILDLTLTNSEGRAVSLRNVARVQP
                     RRGPIRIERRDRERVVTVTGDIAGRDMGSVLADARRALRTIPTPSNFAINFTGDYEEQ
                     QKAFRELLLSITLALLLVYMIMAMLYESLRDPFIVMFSVPLAVIGVVLMLWITGTTFN
                     MQSYIGCIMLGGIVVNNAILLVDYTNLLRRRDGMGLRDAVIEAGHRRLRPILMTAATT
                     IIAMIPLALALGEGAEAQAPLARAVVGGLTSSTLITLVVVPVVYYLFEHKRDGKKKET
                     P"
     gene            complement(29267..30514)
                     /locus_tag="PKV64_01140"
     CDS             complement(29267..30514)
                     /locus_tag="PKV64_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPN15712.1"
                     /translation="MPKQSFFQILRRAKLPVKIALAAVLIAAGWGAYQVFFAKKGPQN
                     GPRNVMVAVETAAIRRGPIRDVGLFSGTLIPKTSFTVAPKISGRIKQLLVDIGDTVRQ
                     GQLVAVLEDDEYRQQVAQAEADLRVAQANLAEAKSSLEMVKRDLERAKSLHAKGIQSD
                     SQLDAARAQYEAQEARSNVAQAQEANRTAALETARLRLSYTRIQAAWERGSAVRYVGE
                     RFVTEGSMLPANGAILSIIELHPITAVIFVTDRDSYRLQPGQETNILSTAFPDTAFIG
                     RVARMSPLLNETSRQARVEIEIENPNGLLKPGMFITAEIEYDRRAEATIVPLSAVVQR
                     AGAAGVFLADLKTRKAVFVPVRTGIVSGEWAEVVEPAGLTGQVVTLGQHLLEDGVGLI
                     LPQANGGGPPAADPAGKPHGATR"
     gene            complement(30574..32157)
                     /locus_tag="PKV64_01145"
     CDS             complement(30574..32157)
                     /locus_tag="PKV64_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPN15713.1"
                     /translation="MKRPTRAAIPLLLAVLSLPAPAVQEGENLNLSLPECIQKTLNNN
                     LGLAAEVLTPQIAEAAVALAGEKFIPSLALSYSRQDNISASYSFLDAAGAAVSTLQND
                     YSSQFSQVLPTGGTLSMSLIGYKSDTTRQFQTINPRFGSTLRFNFSQPLLKDFGWKMS
                     RREFILARNNRDMSEYNLSETMEEYVYRAKSAYWNLVYSRENLNVRRQSLKLARELLE
                     KNKAEIEAGTLPPIEILTAQADVSTREADILEAEAMVRNSEDLLKTLMNLEAENTRAA
                     SLHIIPTDSPSVSKPDVDLDRALREAYENRPDLQAARVGMETSRFNLSYARNQTAPDL
                     RLQASLWSPGISGTQIIYENNNALSGKIIGVVPAGGGQALKDAMDFRYKNWSLGLSLN
                     IPLSTVFSRAFVAQADLALEQAQLRMKNQEQQIFLDIRTAVRAVETNYLRIQAYRVAR
                     ENAAKKLEAEEEKLKVGLSTNYFILQYQRDLANALTMELKAVVDYNVSLAGLYRAQGK
                     GLEMERVPLPGESGGPAER"
     gene            32384..33118
                     /locus_tag="PKV64_01150"
     CDS             32384..33118
                     /locus_tag="PKV64_01150"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPN15714.1"
                     /translation="MSTAEAGVLLLALAGLFFSGGGPAPGRDENTVFARRRQAMVAGQ
                     LASRDIRDKRVLEAMGRVPRHLFIPPESRELAYEDFPLPIEEGQTISQPYIVALMTQC
                     LELRGGERILEIGTGSGYQAAVLSVLAAKVYTIEYHASLAGTAAARLAELGYANVEVR
                     SGDGFFGWPEAAPFDGIIVTCAARRLPKPLFDQLTEGGRLVIPLGRAADVQRLVRVRK
                     VKEKPVEEEITLVRFVPMLGADKNKR"
     gene            33202..36498
                     /locus_tag="PKV64_01155"
     CDS             33202..36498
                     /locus_tag="PKV64_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPN15715.1"
                     /translation="MSKKSWMFALLFSVIALSVIFAETTEARLLRFPAASGEAIVFSS
                     GGDLYTVPLEGGLARKLTSHPGYEMFPRFSPDGKTIAFTGEYDGNREVYVMPAGGGIP
                     RRLTYTATLGRDDVSDRMGPNNIVMGWRPDGRSIVFRSRMREWDDFIGQLYVVPAEGG
                     TPVALPLPRGGFCSFSPDDSKLAYNRVFREFRTWKRYRGGMTDDIWVYDFETKKTENI
                     STNEAGDIFPMWSGETIYFLSDRDENKRMNLYAYDVKTKETRALTRFADFDIKFPSLG
                     PTAIVFENGGFIYRFDLASQKAARVPIFIEDDQVSARGRIANVASRVTNYEISPDGKR
                     ALFGARGEIFTVPAKYGNTRNLTNTSGIHERNSKWSPDGRWIAYISDASGEDEIYLRP
                     QDGSGEAEPLTFKADTYKYGFLWSPDGKKIAWTDKLFRLQYVDTATKQVTVVDRAAVG
                     EMGPFTWSPDSRWIAFTKPEAEGMPKVYLYGLDGGKTAAVTDGWYSSYEPAFSGDGKY
                     LFFVSDRDFNPQLSEIEFNYSYSSMARIYCLPLAADTPSPFAPKSDEVEIKKAAEAES
                     PKSGAKGKPAEPKKSEPEKPAGVTVKVDLEGLKDRLIGLPVEAANYGGLASVGDKVFY
                     LKFSRGPGGGPAFKSYSLSDRKESDLGQIDGYEISADQKKMLVSQGRSYAIIDLPQGP
                     VKIEDRLDLSGMDMALDLKAEWEHIFLESWRQMKYFFYAPNMHGQDWDALREKYLPLA
                     QAAGCRADLTYVIGELIGELSAGHTYVGGGEMPDLPKVKVGQLGAVLVKDASGYFRVD
                     KILRGENWDRSLRSPLTEPGVDVKDGDYILAVDGRPTKEMTDIYASLLNTVGRQVTLT
                     VNGKPEDKGGRRVVVVPVADEQKLVYYTWVRENIAKVDKATGGKVGYVHIPNMGLDGL
                     NEFVKYYYPQLRKKALIIDVRGNGGGFVSPLVFERLRREMAMIEMGRDTIARPDPGDV
                     LVGPKVCLLNEFSASDGDIFPYRFKKAGMGKLIGKRSWGGVVGIRGTLPILDGGVLNR
                     PEFAPYNMEGKWIIEGVGVEPDIVVDNDPAREYAGIDDQLNKAVEVILEELKTKEVTL
                     PGIPPYPIKK"
     gene            36883..38325
                     /locus_tag="PKV64_01160"
     CDS             36883..38325
                     /locus_tag="PKV64_01160"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPN15716.1"
                     /translation="MKDISGSTDLTGTDLKHYRIEAFIGQGGMGVVYRGRDTRLNRPV
                     AIKLLSPALVASAERRNRFVQEARAAAALSHPAIAQVYDIDEVDGCLFIVMEYIDGRS
                     VGRLIADRELDLLSSVEIAFQVVEGLEKAHEAGILHRDIKSDNIMVTRDGRAKLLDFG
                     LAKLMEPAPAASAPPSDPLRTLTKDAGRTLPGLVMGTVPYMSPEQARGRDLDVRSDLF
                     SLGVVLYEMVTGELPFKGETPLDTMHAIAYEEARPVTVVRRNLPPQVHRIVSRCLRKR
                     PEDRYPDAETLASDLKRFKLDLESGSRTDLRPIERLQAWFDRMISSVPLGAKGVWIVA
                     GVVVLAVLLLFTSFNWGSLIAPAVIGVFLYRGIRNRKKRLLADFAKKVSRMPSVEAVI
                     FHDGKMTTVVKDAPASAFVRITGLIEEMNGKLFFGKPVAAEIASDLPAEEFQALIRRL
                     GVLYVRTERPGEPPAGKQGKNKDHSFSGMK"
     gene            38356..38568
                     /locus_tag="PKV64_01165"
     CDS             38356..38568
                     /locus_tag="PKV64_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15717.1"
                     /translation="MNPDPRTIGDVLLRRNAENSARLDFTRVDAYPENILNRILWHAV
                     RGSGAPYPAWAVSAEREDGKKGAPTR"
     gene            38657..39532
                     /locus_tag="PKV64_01170"
     CDS             38657..39532
                     /locus_tag="PKV64_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15718.1"
                     /translation="MKREFTAAFLLTVMAVSWAAAAPPASDMDIWKEFLQLLRSGTLT
                     VDWIHPDDPRTRESRLTNLLDWTKDEDWSEWRATPKVVRQDNLVSFIIEVGKKRRAPW
                     TYVFCFTVEGGRWYYRFVEGVFLRLDQVVSLPADASSFPDLSEERKNWMRQEIYWTKI
                     VGLFTEMTRLKGTEYALGFLRDGAEYVQAATSWIPYFPASRAFILYLCWEQARMQGNK
                     VVLEKLTDREAVVRFDDHQFFALYMQTSHLREQIALGDYMGIFEAIWQDRARAAGWNL
                     QIDGQGPTIYLRFSR"
     gene            39739..41250
                     /locus_tag="PKV64_01175"
     CDS             39739..41250
                     /locus_tag="PKV64_01175"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPN15719.1"
                     /translation="MNARRLNALILAAALLSPALSAATRDGRNFDCYTIVVGKAASAD
                     GSVLLAHNEDDWGDNIVNVRRIPPGDYGRERNVALGRGAHYRTDGRTAGFLWIEVAPQ
                     EYSDSFVNAHGVVIVSDSCPTRETESDLTDGGIGYMLRRIVAEKARTAREAVALAGAL
                     IEQFGYRDSGRTYAVADGNEAWLLAAVRGRRWVAQRVPDDEVAILPNHMTIRRVDLGD
                     SVRFQGSADLVSYAEARGWYDPEKDGPFDFKKAYTRPSTDLLADHNTLRHWRGLELLT
                     GREWPIGENYPFSVKPAEKVTVERLMAILRDHHEGTPYDATDGYKTGSPNRAKFRTIC
                     TATTVTSLIASLDASRPGPLAASLWLTLGKPDTTVFLPLYGAGGALPAGFGLGPQTHD
                     DEAFYRQHFEEAGFKAARGMLLNTRVLAVEKAVEADYGAKIEAVRKGIEILERSFLDG
                     RSAFEAALSAQYAVNKAEALKSVDDYVAAAFDRAAEMTERLAKTLGLPSVPSH"
     gene            complement(41422..41763)
                     /locus_tag="PKV64_01180"
     CDS             complement(41422..41763)
                     /locus_tag="PKV64_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPN15720.1"
                     /translation="MKKSKGSDPQLTAGPLDLPGLADYQEGAIVSRTIIDKDAGTVTL
                     FAFDKGQKLSPHSAPFDAFVQIIEGRALVIIDKEEYPLSAPAAILMPADIPHAVQALE
                     RFKMLLTMVKS"
     gene            42051..42296
                     /locus_tag="PKV64_01185"
     CDS             42051..42296
                     /locus_tag="PKV64_01185"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HPN15721.1"
                     /translation="MSAEETKLIEVRRVWGLTEADIIKAFLESNGIDCVFQGRNVLTV
                     YPFTTDGLGEVRIMVKDEDLEAARALLEETDLFSESE"
     gene            complement(42334..43773)
                     /gene="ahcY"
                     /locus_tag="PKV64_01190"
     CDS             complement(42334..43773)
                     /gene="ahcY"
                     /locus_tag="PKV64_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPN15722.1"
                     /translation="MNTEKKTVLPLDRSLPYKVADLSLAGWGRKEIRLAEAEMPGLMA
                     VREKYGSQKPLRNMKVMGSLHMTVQTAMLIETLRELGADLRWASCNIFSTQDHAAAAV
                     AEAGSAAVFAWKGESLEEYWWCTEQSLTWPDGSGPDLIVYDGGDATLFVHWGVKVEKD
                     SSLLDRTYDNRELRIVIDRLKESVRRSPGRWTAAAARIRGVSEETTTGVHRLYQMQAE
                     GSLLFPAINVNDSVTKSKFDNLYGCRESLADGIKRATGIMVAGKKVVVCGYGDVGKGC
                     VQSMRGFGAQVIVTEIDPICALQAAMEGYPVVPLDDVVSGGDIFITATGCCDVLTGAH
                     MERMKDEAIVCNIGHFDSEIDMHYLENTPGCVRENIKPQVDRWTLKSGRSIIVLAEGR
                     LVNLGCAQGHPSFVMSNSFTNQCLAQIWLAGEKPALGVHTLPKKLDEEVARLHLGRIG
                     ARLTKLTEKQAGYLGIPVDGPFKPDHYRY"
     gene            complement(43803..44732)
                     /locus_tag="PKV64_01195"
     CDS             complement(43803..44732)
                     /locus_tag="PKV64_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPN15723.1"
                     /translation="MKDGKTSDSLRSLKALADETRLRLFQLGRSFELNVNEIVETLGM
                     GQSRVSRHLKILAEAGLLTSRRDGMWTFYRTAADGPAARFAAAIGEKDRGIFARDLAR
                     ARKILEDRNRESRRFFDAIAGDWGEMKRALIGDAALDPLLLRHVPRGEVAADLGCGSG
                     DLFAVLRRKAGRVIGVDRSPRMLEEARRRHLSDSRRGILELRMGELESLPLRDGEVDC
                     ALMCLALHHLPDPGRGLAEAARILKPGGTLVVVEWSAHQDETLRTRFQDRWLGFEPQT
                     LEDWLTGSGLSVRTKTTLSLPTGLKILLFRGRK"
     gene            44839..45966
                     /locus_tag="PKV64_01200"
     CDS             44839..45966
                     /locus_tag="PKV64_01200"
                     /inference="COORDINATES: protein motif:HMM:NF015376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HPN15724.1"
                     /translation="MFFLGLFAGWAFQAALAAAPAPVSGGLAPAPFFSALASPASLLD
                     LDDDALRRRVEENPGSLGSLSLGTPDSAVVINSVSLPADPRWEFLPRAECWATAETVE
                     AVRAVVAKVHEAFPGTPPIVIGDIGDPDGGRLKRHASHQAGRDVDFGFFYKTGTASGF
                     PVGTAATLDLPRNWALVRAILTCTDVEVIFLDTGIQKLLYGYAAAIGEDKAWLDRVFR
                     YRKGSSSAVIRYARGHRTHYHVRFYNPVAQELGRRVYPMLVQAKKIKPPVFTVRHVVR
                     DGETLGHLARRYGTSVRAIQQANGLSSSMIRAGRSYRIPLKGVSAPPVKPLIVPSRLL
                     PPSVPDVLAAASWPSAAAFDRQALLKLMEAAIQGAAVVRRV"
     gene            complement(45963..46952)
                     /locus_tag="PKV64_01205"
     CDS             complement(45963..46952)
                     /locus_tag="PKV64_01205"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPN15725.1"
                     /translation="MFKPEEIIFAPTADCNLACPHCRVTRIPDRLEARDAVAFMKSCR
                     AAGLERVGFSGGEPFLRPDFLNEVSRAAVEMDMLFGRLMTNGVWFDSPDHLRRTLTGL
                     REAGFDGKFGVSIDAYHDQDIRDLTLFFETVFSVWGRRDGCEIVTVIPPDGAEPRETF
                     ERLAERFDGRLIIDEGIPYSIVNDAFMNPRETADGPFEALAIDIIRVPYSAPAEENAW
                     TAEHWFEDDFCRGPGNVFYVHPDGAVAVCCGFANENGGLIIGRIPADGYRQLLRRAQA
                     SPVVRACFDTGLATIRELLEAQGSSFPGKTEDRCFFCDYLAKHDFPIPKQRNR"
     gene            complement(46952..47536)
                     /locus_tag="PKV64_01210"
     CDS             complement(46952..47536)
                     /locus_tag="PKV64_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019867334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPN15726.1"
                     /translation="MVKSFGMFKASDLKKNNVVKIEGDPCLVETVNVQKPSARGAATL
                     YKFRFRNARTKRKTDVTVRGDDLFDEAELERRPVQVLYGDALSFSFMDLEDFSQFTLA
                     KEEIEEEWPYLTEGLEGVLALSSEGRILGLELPTLVTLPIVETRPSVKGGSVTARTKP
                     AVLSTGMAVQVPEYLETGDLIRVDTRTGLYASKA"
     gene            complement(47687..47986)
                     /locus_tag="PKV64_01215"
     CDS             complement(47687..47986)
                     /locus_tag="PKV64_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPN15727.1"
                     /translation="MPKKPMTKSQIVAHFAEKFEMPRKAAGEFLEELVDLAIAETKKA
                     GSFTLPGLGKLVLVKRNARVGRNPATGATINIPAKTVVKMRIAKTAKDAIVPPKQ"
     gene            48142..49494
                     /locus_tag="PKV64_01220"
     CDS             48142..49494
                     /locus_tag="PKV64_01220"
                     /inference="COORDINATES: protein motif:HMM:NF015672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15728.1"
                     /translation="MDEGARMRRTFAVSTARLRIMMEAFRGEMRRGLNGKPSSLRMLP
                     AYVDRPTGRERGTFLALDLGGTNVRVLQVDLPGDGRLPVCAEDRFVLSRDKVTADGSV
                     LFGALARFIGRFLRERRLSGRLPLGFTFSFPLRQASIAGGELIAWTKGWTAEGVVGRD
                     VAALLNRALAAERISGVRVVSLNNDTTGTLMARAYLDPDCDAGCILGTGTNICYRERT
                     SEIRKPVGPHGRDHMIINMESGNFNAVLPRNRFDVLLDRSSANPGLQAEEKMVSGKYL
                     GELARLAVVELCGRGRLFGGRLPEIFSRREAFGSERLSEIEKDDSASADGVREFLWDW
                     GARPAAAGDARLLRDVVRAVSGRAARLAAATLGATVTKNDPALEAKHSIAVDGSVFHK
                     YPGFKARMEETLRELFGGRGGRIALAPMRDGSGLGAAVIAAVAAESAKTGGKKPGAGW
                     "
     gene            49689..49991
                     /locus_tag="PKV64_01225"
     CDS             49689..49991
                     /locus_tag="PKV64_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPN15729.1"
                     /translation="MNIYVGNLSFSVSETDLREAFAAYGQVATASIIKDKFSGESRGF
                     GFVEMPTKEEAEKAIAALNGKDLKGRTMTVNEAKPRTDRPRTGGGGRGGFGGGRRF"
     gene            50130..50330
                     /locus_tag="PKV64_01230"
     CDS             50130..50330
                     /locus_tag="PKV64_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15730.1"
                     /translation="MAKSSRAYKSAKRSKELDRLRKREEKRRRRMAVKSGGAAEEQVA
                     SAETAAEQPAPEEDPDPIIPRD"
     gene            50534..51514
                     /gene="pyrB"
                     /locus_tag="PKV64_01235"
     CDS             50534..51514
                     /gene="pyrB"
                     /locus_tag="PKV64_01235"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HPN15731.1"
                     /translation="MPSKPKNPHLPFGDQQTAPWYGKNILSVKQFCRNDLEYIFSVAH
                     EMRTMVERVGTFNLLKGKILANLFYEPSTRTSSSFMAAMERLGGAVIPISEVKFSSVA
                     KGESLPDTVRTLGCYADVIVLRHPEIGAAALAAKYAGKPVINAGDGAGEHPTQALLDT
                     FTIREELGRLDNLNVTMLGDLKYGRTVHSLARLLTQFNGIKLNYVSPDILRMPPDVME
                     EVAAKGLPQSEHSTLDKVLPETDVLYVTRVQRERFEDPQAYEKVKNVFVVNPAVMKAA
                     GKTMIVLHPLPRVTEISLDFDDDPRAAYFRQMEYGLYVRMALLAMVLGKA"
     gene            complement(51534..52757)
                     /locus_tag="PKV64_01240"
     CDS             complement(51534..52757)
                     /locus_tag="PKV64_01240"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HPN15732.1"
                     /translation="MRAPFDIAVVGGGASGLTAAIAASREGRSVAIVDRMPRLGKKIL
                     ITGGGRCNLGNERLDPGLFHSTNPGLVASVLARFGTREIVSFFEGLGLRIVSDGDRLY
                     PATNQAASVLKVLEMEVRRRRVSVIPGFEAVSIIRSAEASRAAASDGRTIEARAFILA
                     GGGRSYPALGSDGSAYALARTAGHRLIEPVPSCIPLLVRDPWCHFLQGQRVRAQAEAW
                     IDGQRAAEALGDVLFTAYGLSGTAVLDVSTDISIALHRGKKARAEVVLDLLPDMSAEA
                     LSAELARRLEGGWGAADLVSGLLPEKFGRTIAGLIPGGPPRPARTAAALAAVLKGKRF
                     AVQGTRGWNEAEFTSGGVDAGEIDRERLSSKKAPGLYFAGEILDVQGPRGGFNLSWAW
                     ASGTLAGRAAAGETG"
     gene            53154..53390
                     /locus_tag="PKV64_01245"
     CDS             53154..53390
                     /locus_tag="PKV64_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15733.1"
                     /translation="MQTGRGSGVMADPQQVGTDAEVGAEALAPQAGQTYVVSFMVFLS
                     RKYYSRLASRRPVLRLGTSAASAEKNAARFRLSV"
     gene            complement(53374..54435)
                     /locus_tag="PKV64_01250"
     CDS             complement(53374..54435)
                     /locus_tag="PKV64_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPN15734.1"
                     /translation="MTGSTLDRDFEKSRKRIARCLSRYFDGRAAGLAAVNAWGADFTR
                     RIRPFVLGGKMIRGGLILIAHDRLDGKAGPDAVKAAAAMELFHSAFLIHDDIMDDDPL
                     RRGKPSIHVQYAAQARRKGVNDAPGFGRAAALCAGDVLIFMGLEILASLAAPAPDKAR
                     VLSLFGRELAHVGPAQVQDVYSGRIRRPVSRAGIYRLYAFKTARYTYSLPLAAGAILA
                     GAGQSAIRGLEDIGLDLGLVFQIKDDEIGMFGSPSTTGKPVGSDVGEGKKTLYYFYLS
                     RARGPRAAALFGKRRPAAGDISRLNALLRTEKIPERIEADVRRIAGRCARRIREASLP
                     SSMKTALSELLASSLKRKA"
     gene            complement(54432..55313)
                     /gene="ispH"
                     /locus_tag="PKV64_01255"
     CDS             complement(54432..55313)
                     /gene="ispH"
                     /locus_tag="PKV64_01255"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPN15735.1"
                     /translation="MNKSASPKVDVSPYVGFCGGVNRTLKIIEKLRLEQPGKKIYLLG
                     KVVHNEIVLNRMKRRGFKTIDDHRLAEDGLLIIQSHGIPASRLSEIKASGISFVDTTC
                     PMVKDIQARARRLAGEGRRVIIIGDRRHEEVQGIAGQVENALILGTPEEAGRGFFQGV
                     DRAGVVIQSTFIRAEADAILRKIRRFVPDVRFENTICKPTSDRQRDARIQAARYDGVI
                     VIGSPASANTRNLMSIAGAGNRRTRLVSRPEDVDGLPLGQGRKFYVLSGASTPMDVID
                     RTVARLRRRLERETKSR"
     gene            complement(55346..55921)
                     /locus_tag="PKV64_01260"
     CDS             complement(55346..55921)
                     /locus_tag="PKV64_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15736.1"
                     /translation="MMTVFPPDVRFSSRRNFPFQGVIDSSFSGRISQIAATAVSKPGK
                     PPVKRKKRPWRAKCRVVNPPAEENREDRPLATIPSGGRVLFSPNTDHRFESEKEIRRP
                     NEKLIARGRGFHKSGMNVIGLANLDKERFPFVWERRDQSAIQRGAGLLVSVGFLWYKF
                     LLDMIPEGKMSRRTDHGGLGRLCPGGRRKPR"
     gene            55952..57235
                     /locus_tag="PKV64_01265"
     CDS             55952..57235
                     /locus_tag="PKV64_01265"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HPN15737.1"
                     /translation="MEKPFNAFEMAQKQFDAAADKLGLDQATRELLRNPLREYHVLIP
                     VRMDDGSTKVFRGYRVQHSDARGPCKGGVRFHPHETVDTVRALAMWMSWKTAVVDIPL
                     GGGKGGVICDPHNLSDREQEGICRGWVRQLAFNMGHEQDIPAPDVMTHGQHMLWMMDE
                     FERIRGAKFPGFITGKPVGMGGSLGRKEATGFGVIFTVREAMKELGIDIKKAVGAFQG
                     FGNVAQHAVKLFKEYGGKPICVACWDQADQTSYTFRRESGIDPDELVAITDKFGTIDK
                     AKARQAGYEILPGDAWIEQEADLIVPAALENQITAANVGKIHKRVRIIAEAANGPTTP
                     DADAEIIKRGIFVIPDFLCNAGGVTCSYFEQVQCNMNYFWPQDEVLSKLDEKMTVAFK
                     AVSELARKKNIYMRDAAYLIAVTRVAEASHKRGWV"
     gene            57321..60113
                     /locus_tag="PKV64_01270"
     CDS             57321..60113
                     /locus_tag="PKV64_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPN15738.1"
                     /translation="MSLRESDVPKGPLRERVKELECLQAIVTVIETPGLSSDKMLQRI
                     VDVIPSGWQHPERCRARIKIHKAVFHTPGFVETPWMQRVPLRARGETIGALEVCYTDP
                     GGSPQENAFLLEEKKLIDLIGNRLGRLIEEGEGAGRAAAAGPAEEFPGKRKPEWRVIM
                     DLLRETDPMLYRRVLRRLMNHLHWQGIPGVQGLLFHLTPESYSRAEAGPDENQPQPVQ
                     SVERLSKVFEEALWIASLALTDAELSDLLKQWMRQDKLGFFMVATEKREISVVEIKEI
                     LNRFCRSTGEDEQSLSPADDLQARIALIRRFLSERLPFIRIARDFMTIHDFGRLLPRV
                     FGPTSGTGKLGGKSAGMILAEHILRQKAQSDPAVGTIKIPESWFITSDGVMDFIHYNY
                     LEDFQSFKFRPIEEIRHNYPYLVQVCKQSFFSPDMLAQLKLILDDAGGDPLIVRSSSL
                     LEDSRGSAFSGKYRSLFLANTGTKEERLDALTDAVAEVYASIFNPDAIEYRAERGLLD
                     YYEEMGVLIQQVVGTRVGKYFFPAFSGVAFNNNEFRWSPRIKREDGVLRLVTGLGTRA
                     VDRVGNDYPLLVSPGQPGIRVNVMPDQVIHYSQKAIDVINLETRRFETHAIEELLKEI
                     GADFPIWDRLFSVWDGQSVRRANRVLDQPDQDSVVATFDGLLTGTPFLLQMREIMRVL
                     KEIFGIPMDVEFAHDGRDLYILQCRPQSRLDDEEAIAIPAMIPDEGKLFSARKYITNA
                     DVRGIRTIVYVDPEGYGRIASLADMGAVGDAVSRLNSFLPRKSFILMGPGRWGSRGDI
                     RLGVRVAYSDINNSAMLVEIARKVGEYVPELSFGTHFFQDLVEARIRYLALYPDDRDV
                     IFAWDFFRKSPNVLTRYLPEYAYLAPVLRVIDVPETADGRELRVIMDGDREMALGFLC
                     DPTKGRGEETGDAGLL"
     gene            60159..>60660
                     /locus_tag="PKV64_01275"
     CDS             60159..>60660
                     /locus_tag="PKV64_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPN15739.1"
                     /translation="MTARLLLVNCYRENAAKKIDPYREWIQAGARDLGLKLEILAKFD
                     LGPLPGGDYDGLIVSGSRQMIGRGQVEAGLLDFLRTNRRPLLGVCYGHQALARAFGGL
                     AGKDAATHRGDEMIHLEAPDALFAGFPASFPMSESHEEIVARDEALEKEFLVLAAGDS
                     GRVEAVR"
BASE COUNT        11330 a        18468 c        19144 g        11718 t
ORIGIN      
        1 cctggacagc atggcggcct tggggctcca agctgaacgc cttgtaaaat gtagcgatgt
       61 ggtagatccg ggagagcggc acgttcaacc tttcagaagc ccagatcaag gccggcttgg
      121 gaagccagtg gttttcggtt tggatatcga ggagaacctg gatgagggcg ttctcccggc
      181 cgtcgtattt gtccagaatc ctgtcgaccg cggcaagctc ctggaccatt tggtggatgg
      241 cttgcttttt gtcccggact tgcttgaggg actcggccag gtgcatggct tccgtttcga
      301 ggctgcgaag atcaaagctt agggggggct tgtcgaggtc cagcgagtag tactcgctca
      361 agagctcaag gcctctgagc agaacatcgc tcttgctggc tttcgagtct ttttgaaggc
      421 ggctcaacgc ttcgttctgt ttttcgctca aacgcattcc gacgaacttg gatttctttt
      481 ttgttgaacg cggcatttga tactcctggc gtttaaccgt tttacataat tattctaata
      541 cttcttgttt ttgttgtcaa caaggggacg ttttgggaaa tatccgagat gaatttaacg
      601 taacgtttaa cgtttgttta agagttggag gtcgcttttc ggatattttt ttaagtaaaa
      661 gttgatgttc attaagcgtc gatgatgaga aactcggcct tgcccagccg gttcatgcgc
      721 ctctgatgac cgatcaacga cagaagctcg gcgagaatag cggtttcccg gtcgttattc
      781 cggagaacgg gatcgttcgt cagatcgcgc agggcgactt ccagcgcttc caccggcgta
      841 ccggagggaa tgctcaggag aaaacgcgtg ggggcgattt cgatcaactt caaccagggg
      901 atttccttca gggctttgct cggtccgacg acgatcaggg atttgccctg agccatctcc
      961 gtgagcttca ccggtttgaa tcgatccgcc tccgctccgg cgacggcttc cgcggctttg
     1021 gccacggctt gagcgcggtc cggatcgatt tcagcgagga gatcaaggat gcgcaccggc
     1081 aaggtcattg tcacggggcg gcggggctcc cggaatttcg ggggccggcc gctgcgattc
     1141 gatttcacct gttcgggcat agcttcctgc ttcattatag caaatcgtcg aggtcttttc
     1201 gacgcaatga acggaaaatc cgtctccgcc gtcggcggat cgcggctctc gtcttcgtta
     1261 aatcccccgg gaccggcttc ttgaattata acattgaaat ccgttgacaa atacaataat
     1321 aacacaataa actaagcgcc gtgtcgtcga cgattttttt cgataaatcc gggaaagggc
     1381 cttccggatg agatggacga agcaagttaa aagaggagga tcagatgaaa aaactgtccg
     1441 gacaaatcgc gccggccctt ttcgcggtgg gcttgatttt ttttaacgcc ggaatctcga
     1501 tgagccaaac gacttccgtc ctgaaggaaa tgtcccttca aaaaggcgga gcgcggctca
     1561 attttttgtt caaagtcgaa ggagcgtatg tcgtcgaagc ctctttcctg ccttcgccgc
     1621 cccgcttgat cgtagacatg acgccgatat ccgagatgca gattctcccc gtcacgcaaa
     1681 tcggcgatat gggcgtgctc gacgttcgag ccgttcaatc cgggccggat aaaattcaga
     1741 tcattttcga ttttggcccg atgattcccg cgtatggaat cacccaaggc gccgaaggat
     1801 tgaaaatttc cttctggaat gaaggagaaa tcgccgcgat ttcggatcct cgtccggccg
     1861 aggcggccaa gccgattgtc tcaacggcgc ccgccgcccc cgtcgctccg gttcaggcgc
     1921 ggtccgcggc tcttgagcgt tccggctttt tcatcgccgg ccgggccggc ctgtccttgt
     1981 tcgtggggga ggatctctcc gtcgaaaaaa cgtttgacct ttacggggaa acggcgaccc
     2041 tggctgaatc ctattccttc agaacggtcc ccgcgtttga gcttcaattt ggacgatact
     2101 tcaaccgaac caaaatcggg atcggtatcg ggtacagccc gctgaaaagc ccgggatcgt
     2161 tcacggccgg ccttccccat cccttcctcc cggagacgcc gcgcaacgtc tccttcgaaa
     2221 gcgatggcgg gaaaaacacg ctttgggatt tttccgtctt tgctctcttc tccttcctga
     2281 atacagaaat gctttccgtc gcggccgggc cgatgatcgg cttgagcctg ggggccatgc
     2341 agagcatgga ggactttgat ttcaccgagg aatcccctta tgacgccgcc catgtcacgg
     2401 tcgcgaatat gacgtacgcg aaggatcaat tcacggaatt atcctttgga atgctcctca
     2461 gcttggaata ccggttgaac gaagggctgg gccttctgtt cgatttgcgg ggggtttatc
     2521 tgaatcccaa gaacgtcacg ctgggaaagc gggccaacct tctccacctc cagccggtct
     2581 tgggaattca gtacagtttt taacgaggct tcggggggaa gacgccgaaa acggcgcggc
     2641 cggcggcggg tttcttccgc cgccggccgc gtttctttac tggaaataca tcttggcgct
     2701 ttccgtgccg ccgtagccca gggtactgac ggcgaaatac ctcttcacct tcagatcggt
     2761 gaaggtgaac gaggaatcaa acgtgctcgg cgagaccgtc agaatcgggg cgtactcccc
     2821 gtcgccttcc ttcatgtaaa tcgcataccc cttgatgtgg gccggggtat tggccggatt
     2881 gatccgccaa gcgagggagt aggtgatgct cagatttctc cgcaccgagc cgatgacgat
     2941 cgtgagctcg gcgttgaggg ggatctgcgg atcggttttc ttcacctcga tcgttttgcc
     3001 gatttcgtcc atccgtccga gattgtcggt gatcttcagc gtgatcgtgt gcatgccatg
     3061 agtttcgaac acatgggacg tctgacggcc ggccgcggat ccgccgtcgc tgaacgtcca
     3121 ggcatagctg acttccgagc cgttcggact aaaggcgggg acggcttgaa tgtcgaccgc
     3181 cagcggcgga tatccgctgg ccggggagaa tgtgaaatcg gccgtcggca tatcaacccg
     3241 ctcaatcgga ttcagggaat acacccagag gtcggttcct gacgtcgtgt aggaggactg
     3301 ggtcacggcc acattcccca gggaatcggc cgccaggccg tgaaccttgg gatagagcgc
     3361 cgggaaggtt tggaacggct tccagatccc gccgacatta ttggtatagc ggaagccgct
     3421 gccgaagtcg ccgccgccgg tctggcatac gacgtggatg tcgttcttgc tgtcgacgac
     3481 gacccgcccg aaatgctgca tggcgaaggg gatgactctg actggtgttc cgccgatcgg
     3541 caggacatag atttcgcatc cgttttccgc cggcggaaca tcctggtagc agacgatcgg
     3601 ggcattgttc gagccgatgg agatccaggg gtgattggaa ttggtcgggg ttttcggcaa
     3661 ggcgaccctt gtttcccaag cggtcgagat cgtccgggtg gcataataat tttgataggt
     3721 cgagtccggg tctttttcga tccagacggc atggactttg ccgtccggcc cgatgaccag
     3781 cgaaccctgc ttgccgcggc tctggttcaa gatcgcgatg ttatcccaaa ccccattgat
     3841 cttagaacgg gcgaagacga cccctcccgc ctcgtcggtg aaaaagacat ggatgttgcc
     3901 ggcgttgtcc atctgggcga tgggttcgtc gccgccgtag ttggaggcca ccgtctcaat
     3961 cgggccccag atttttgtct tgggatccca gattcttaag tatgtggatc tggtggcgac
     4021 gactccccag gccacgagaa cgtgcccttt ggacgaagtg gtgatatggg gacggttggc
     4081 cttgatggcc aagctgccgg tcatgttgat tggtgtcgaa aacgtcgtcc catcgtaggt
     4141 cccgacccag atcggtccgc cgctgttggc gttggtcgtg ccggggaccc agacgaccca
     4201 gagcctgccg tcgggcgtga aggcgcattg cgtccagcgg attttctccg ggctttgaaa
     4261 gagcagcctg ccgttgccca ggatttgtcc gaattggaga ttcgtgagga gaagcaggaa
     4321 gcaaatgccg agcatgatgt gttttttcat gttgttcgtc tccttcaatt ccaggattca
     4381 tatttccaat cacgttttca cgtggccgtc cgccgaatca aggcgaacaa ggcgcttatc
     4441 ttattatgtt aatattttgc atgtcaatga tttataatgt tataattcat tcgccgcgaa
     4501 tcttaagcag agatcgattt atgccggggg gaaatccggt attccagccg ttcttcgacc
     4561 gccttgagcg cgggggagat gatccgcccg gacgtccgga acggacgttt ttcgcttggg
     4621 cgcaatttcc tccttttctt cctttccgga cgattggaga accgcattga gcgggaagat
     4681 gtatttttcc ggccggtcgt cgagaacgcc gtcgcgatgc ctcccggccg gatgattgtc
     4741 ctgcgcggat tcgcttggat ccgagcgttc cgcgcgcgag gccgctccgg atcgacgggt
     4801 catcttcacg atttcccggc ggctgcggat tttcttcgcg gataaccggc ggttttgcgg
     4861 gaccacggcc ctgaaggcct cgatccgatc cccttgtcct cggttttctc tgcgcttttc
     4921 gcggcgcagg gcggccgggg agtgccgcgg gcgttgaact gggcttgtta tttccgcgta
     4981 attttgcgtc attcccgcaa aattagattt ttccccgctt gaaacggccc cgatagggga
     5041 gaaaaaacag gttttcaccc atagattcac cccaaagggt gattgatcgg aacaggctag
     5101 gagaaatata tgtaagcaat ggaggatatc ccatgagcac gatatgggtc cgggatatcc
     5161 gatcttcggg aaaaaggcgc ggatgatgca gaaaaaagtt cttatcgtcg attatgacat
     5221 gaaggacacg acccgggttt ctgagcttct gacgaattac ggctttgaag tcgccatcgc
     5281 cggagacggc caagccgggt gggatcagta ctcggctctc aagccggacg tcgttctgct
     5341 cgaaccgatg ttgtctaaaa tccatgggtt tgagctttgt cagagaatcc ggtcggattc
     5401 actcggccgc actcaagtga tcatcgttac cggaacttat aaggatccga tctataaaac
     5461 cgagaccctg aggacttacg gaatttttcg gtacttcgtg aagcccgtcg acaacgataa
     5521 gctcctcgcc gccgtcaacg gcgcttttaa tgtcggaaag gaacatgaac ccggacagcc
     5581 ggagttcatc ccgccggtcg ccgcttcctc caagcctccg gaggaaaaac tccggccggt
     5641 cgcggaaaaa aaggaatttg atttcgaaca gccggagttc attccgccgg ttcccgcctc
     5701 cttcaagccg ccggcggaaa agccccggcc ggttgaggaa aaaaaggagc ctgaatccaa
     5761 acagccggag ttcatcccgc cggttcccgc ctccttcaag ccgccggcgg aaaagccccg
     5821 gccggtcacg gaaaaaaagg agcctgaatc caaacagccg gagttcatcc cgccggttcc
     5881 cgcctcctcc aagccatcgc tggaaaagct ccggccggcg gcggaaaagc cgaaacaaag
     5941 gacgcccgat gcggggaatg tggaagtcga tgcgctgttg aaaaaggcgc tcaagggttt
     6001 cgacttggag acggggctga tcaaatcccc gggaaaagaa cccaaaaaag cgccggctcc
     6061 cgagccgccg cgccgtcccg ttcccgcccc ggaaatccgg aagaccgaac cgatcgcccc
     6121 gaaaaaagaa gaaagaccgc cgaaaccggt accaccggcg gctccgcctc cccggacgga
     6181 atcctccgtc cacacggaac ggactccctt cggcgacctg tacgaggaac cggcgaaaaa
     6241 gaatttcaag ccggttgtga tcatcgccgc cgtcgtcgtc ttggcggtcg cggcgttctt
     6301 aattctcaag cccggatcgc cgggagcgac caacgaagtc caggcgggcg gctcctctct
     6361 tctcgaaccc gccgccgact tggaaaccgg ttcgatgctc gactccgaga ccgtggcgat
     6421 cggatccgaa atccaggaga ccgatccgga aaacgcagtg aagcctgagc cgaccaagcc
     6481 cgtcgataag atcgagacgg cccggccccg gccttccgag caaccggcgg ctccgccggc
     6541 cgctcaggcc aaacccgtgg cccggcgccc tgaatctgcg cccgaggcgg ttcgatccgt
     6601 cgaacccgaa gcaacgaaag ttcagcggcc ggccgaaacg gtcggagata agagagaaag
     6661 cacgcccgcg ccggcggctt caacccccgc tgaaaagccg gttcaagaga gcgcccctcc
     6721 tccggaagtc aaggagtccg ttcctcccgc ggcggctcaa gcggtcactc ctccgccggc
     6781 cgaagtcaaa cagccccccg ccgtcaaacc gtctccgcct tccgttcaac gaggccagtt
     6841 agtgccctta aatgaagtca gccaaaaacc ctccgtcgtc agaagggtca tgccggtcta
     6901 ccctgaaatc gccagaaggc ttaaaattga gggctcggtc accgttaacg ccttgatcga
     6961 cgaatcgggg aatgtcattg ataccgaagt catcaaggga ataaaaaatg acagtggaac
     7021 gcacagggcc gccgaggcgg ccgtgcggaa atggagattc aatcccgccc ttgtcaacgg
     7081 tgttcccgtg aaggtctgga tgccggtggc tctcgtcttc aaggtcgaat aggaggattc
     7141 gcgaaaaagg gagaaatcca tgtccatgac caaagatcaa gaggcgcttt atcggcggac
     7201 gctggacgac gtcaaaaaac agctcgatca gctggacgaa gaagtggaga tcgagctgca
     7261 gaaagtcagg ctcagactgg ccgaactcca agaatccaag aagtctttga aaaaggtata
     7321 cgaaggcatc gccaacctgc tcggcatcga gatggaaaaa gaggctgacg aggaccaggc
     7381 cgcggcgatc tccaaagttt aagcggccgc cccggccggc gtcgtgaacc gtagcggccg
     7441 ggacgccccc tttcccgcga ttcccgcaag atccggtttt tcctttgtcg tttccgttgt
     7501 ccgccggcgt ccgtgaccgg tcccaagacc ggccctggag ccaatcctct ttcgccgttt
     7561 cttgacttcc tccaggcgcc gttttatctt ttcgtcaagg cgggaaaggc gtcgatccct
     7621 tatgagcgcc ggctcgaaaa cgattctgtt tcttcttctt atcttaatga tattcttcat
     7681 gttgtctttt tctttcgccg ggcgggacgc cttgccggaa tcgaggcatt tcacgctcga
     7741 aaagccggcc gagggcgttt acgcggcgat ccataatgac ctgggcggct tcgccatctg
     7801 caaggcgggg aacatcgacc tgggggatcg aaccgtcatc ctcgatccct tcatctgtcc
     7861 gacggccgcc cgggacttga agcgccaggc cgaattcctg accggaaggc ctgtttcgct
     7921 ggttctcaac ctggatcctc acagcgatcc catcggcggc aatcaggtct tcattcccgg
     7981 ggccgtcatc atcagtacgg cgaacgcccg cgcgtatatc gaaactcatt tccagaaaga
     8041 tctcgatacc caaaaagaga cggttccggg cgaattgaaa atcgttcagg accggctcat
     8101 aagggcgtcc ggaatcgaaa gattcgagct ggcgttgcgg gagtccgaat acaaagcctt
     8161 ggtcgatgcg tttcccgaga tcgaaatgac tcttcccgat atgacgatcg agggcccgat
     8221 gacgattcat ggctcgaaaa gaaacatcgt cctcgtgccc gtcggacggg ggcatacgac
     8281 gggagacatg atcgtctctc tccccggtga ccgaatcgtt ttcatgagcg acgagctctt
     8341 tgtcaaacgg cacccctatt tgggggacgg cgatccggag tcgttgctaa gcagtctgaa
     8401 gaggattctg gccctgaacg cccggccatc gccgtgccgg gacacggtcc cgtcggcggg
     8461 gcggattcgc tgcgagagat gatcgattat atcgagacgc tcacgaccct ggtccaggag
     8521 gagatccgga tgggcgccgg cgagaacgat gttccgcgcc ttcccgtttc ggagaagttc
     8581 aaggactggc tgatcgaggg cttttacgtc gtcaatctcc ggtttttata tcgaagcatg
     8641 attccccggc ctgatccgaa gcggacgcgt ttgccgtcgc cggggaaagg ggccgaatga
     8701 atccgccggc acgaatatgc tatagtcctt tgggagagac tcctcccgag ggcgtcccgc
     8761 aagggacgga gcggagggcg cggtattgga aggagggcat gatgccagaa gaagcgatcg
     8821 gcaaagtgac gcattatttc gaccggatcg gagtcgccgt cctgcagttg acggggacgg
     8881 tccgggtcgg ggacacgctt cattttcaag gacactcgac cgatttcaac caggcgatca
     8941 catcgcttca aatcgatcat aaaaacgtgt ccgagggaaa tccgggccag gacgtggctc
     9001 tgaaggtcga ccggaaagtc catccgcacg accatgtcct gaaagtgaca gcctgatcgc
     9061 gggatccggg gcggatcgtt cttgacagcc ggccgcggcc ggagcgttcc gtttcccaag
     9121 aaagaacggc ttgggctgtt tttcccagcc gagggatgag ggattcgccc gaagccgggg
     9181 ggctgtccct cgtcaagccc cgcgtcctgg aaagccggtc gccgccttct tgaattcagc
     9241 ccttcgccgc ggaaccgatc gcataaaggg ccggatggtc gcccaagcgg ccggaggggc
     9301 cgcggaccat tcgccaggaa aaaacaccct cctcgaagcc gggccaggag cgcaatcggc
     9361 gggcggccgc ggcgttggct tccaggacgc cgacgcggaa gatttcatct ttgtgctccg
     9421 aggccaagtg ctccaggagc ggcccggcgt cttccggttc aagagcggcc cagggcccga
     9481 tggccagcaa tccgcctccc ggccgggcca tgatccagcc ggagagtcgc ccgccgcgtt
     9541 ccgagacgag gctgagatcc gggaatcggc cggccctccg ccgcaggaaa aagccgcggt
     9601 cgtcgccgaa cagctctcgg tccagggcgc acagcggctc gagatcctcc ggatcgaggc
     9661 gccggatatg atcgtgcttt ttgccggcaa tccttccccg gaagcggagg gagcggcaga
     9721 tcttccggaa tccttgcgcc tcgtagaagg ggaccgcgtt aagatcgccg tccaggagga
     9781 tgttttccaa gccggccgcg tccaggcggc cgagggcttt ttcgaagagc tgacggccga
     9841 ggcccaggat ccgcaggcgc gggctgacga tcagctcgcc gatgaagccg ttccggccgt
     9901 aggccgcggc gacgcagacg cccgctcgtt cgcctccggc ttcggcgatg aaacatccgg
     9961 ccgcgtcgct ctctaaaaag gcggcgaagg cgtccgggct ctcgcttgac cagccctcgg
    10021 cccgggccaa ggccgcggcg aattcaatgt cccctggatt catttcccgg atggtcaccg
    10081 ccgggatcgg gctcacggaa cgcctccgac tcccggccgg ccgcgccggg cggaaaacgc
    10141 atatcgatct tcgccgggca tgggagacaa aattataaaa gcttgaggac aaaggcgaac
    10201 gtcatgacgc cggacaaggc caagcggaaa cccatagcca gaagaaatcc tataaacgct
    10261 ccccatccgg cccgcagagc cgcggggctt tccttcccgg ccgcaagctc cccgatcacg
    10321 gcccccagga acgccccgat gatgatgccg agcggaggaa aggcgaacac gccggcggcc
    10381 atgccgatga aggcccccca gaagccgcgc ttgctcgagc cgtatcgctt cgtcccggaa
    10441 agaggaagga cataatccag gatcatgctt ccggccgtca gcaggcccat gaccacgaga
    10501 aacgccgggc tgaacgaatt ccagccgcgg gcccagtcga gaaggatcaa accggcgaaa
    10561 ttgagaggcg ggccggccat gacgggaagg acgcagccag ccaggccggc cgcttgaaga
    10621 actcctccca ggacgatcag aagggcggcg gacacggctt cccttcctcc tttccggtcg
    10681 atcggcgttc cgggcgcagt ttaccatgtc cggcggcaaa acgggaagcg ccggtccgag
    10741 gcttcctccc gtcccttttg acgatcccgc gctgccggga tagaaaaccg gttgaggatt
    10801 ttccttgagc cggcggaaca gaacgttcgc ggtcatttct ttctttgttc cggaaattcg
    10861 gcggatgatc ctggccgggg cctcccgtcc ggtttgactt aatccggcgg ccggcgacgt
    10921 ccgccggggg gacggacgtc cggaaggcct atccgcggcc gggctgagct cgggctccgc
    10981 acgagggttc ccggcgtatg ctcccccgaa gcgcccggcc gatccgcgtt tcccaagtcc
    11041 ccggctcccc ctcttcagaa cggaggcggt cgatcttctc cgcccccggc cgattccgag
    11101 gaaaataaac ttgacaatgg ttacgaatat gatatgattc cattatcgat attataaagg
    11161 agattcccat gaacgacgaa agcaagcccc ggattcccct gatcggcgag aaagccccgg
    11221 ccttcaaggc cgtgacgacc cagggggaaa tccattttcc ggaggattac aagggaaaat
    11281 gggtcattct attcagccac ccggccgact tcacgcccgt ctgcacgacc gagtttatga
    11341 cgttcgccaa gatggaggag gagttcaagg ccctcaattg ctccctgatc ggcctttcga
    11401 tcgacagcca tttcagccat atcgcctggc tccggacgat caaggaaaaa atcgcctgga
    11461 aggacatgcg gaacattgaa gtcaagttcc cggtgatcgc cgacctgacg atggaagtgg
    11521 ccaagagctt cggcatggtc cagcccgcgg ccagcaacac ccaggccgtc cgggccgtgt
    11581 tcttcattga tccggccggc gtgatccggg cgatgattta ctaccccctg tccaacggcc
    11641 gcaacttcga cgagatcaag cggctgcttg tcgccatgca gacgtcggac aagcaccagg
    11701 tcgccactcc ggccgattgg cgcccggggg acaaggtcat catccccccg ccgggatcat
    11761 gcggccaagc caaggaccgg gtcgagggcg ccggctcggc ctacgagtgc ctggactggt
    11821 tcctctgttt taaaaagctg ccctaaggaa tttcgcctga aggagacgga ttccggggac
    11881 ggatccgtct cctttttttc aacgccggaa gggcggagat gttcgcggtc cccggtgaca
    11941 ggagccgttt cgttgactgt cagcgcggac ttgagtaaaa tctctcgatc cgatgctgct
    12001 ccaatccgtc ttttttctat actatttctg ccttgttttt tgccttgtct ggctcacgcc
    12061 gaaatccctc cgctggctgc cgctcctggc ggcgagcttt tttttcgtcg cctacggcca
    12121 tcccggccat cttctctttc tcgtcctgcc cacgctcgtc gcttatgcgg cggcccggcg
    12181 actggccgcc ttgccgccgg aggcgaggaa agcgcggaaa cggtggctct gggccgccct
    12241 gatcttgatc ctgggctccc tggtcgccgt caagtacgtc aattttttca tggggacggc
    12301 ggccggcgtc gtttcgctgt tcggctcggg ctggacctgg aagccgctca aaatgattct
    12361 gccgatcggc atctctttct acaccttccg cctgatcggc tatttgatcg atgtttattt
    12421 caagcggatc ccggcggaaa cccatgccgg ccgattcgcc ctgttcgcct cctttttccc
    12481 ccaggtttcg gccggtccga tcgagcgggc ggggcgtttc ctccctttgc tgcgtcatcc
    12541 cgagacgttc tcggctgaga ggatcttcag cggcgcctcg caggcggctt ggggattttt
    12601 taaaaaaatg gtcatcgccg accgcctggc cgttttcgtc gacccggtgt tcgccgatcc
    12661 gagcgggcag ggattgaatc tcgttttcgc cgcttggctg tatgccttcc agatttattg
    12721 tgatttttcc gggtatacgg acatcgccat cgggctggcc cgcatgctcg gctacgagtc
    12781 ggcccccaac ttcaaccggc cttatctcag cgtcggcatc gccgatttct ggagccggtg
    12841 gcatatctcc ctctccacct ggctgcgcga ctacctgttc ctgccggtgt cctacgccgt
    12901 gatgcggcct ctcaagcagg accgcctgtt cggggtcaag gtggaatcct gggcctatat
    12961 cacgtccgct ttgctgacca tgaccctttg cggcctttgg cacgggccga cgtgggcgtt
    13021 cgttttctgg ggggcgctgc acggggcgtt catgatcgcc ggccagctcg gccgcaagcg
    13081 tcgccggcgc tgggcccgca aggccggctt gacgtcccgg ccggccctcc tccgggccct
    13141 gcgggtgatc ctgacgttca acctgatctc gttcgcctgg attttcttcc gcgccccgac
    13201 gctttccaag gccttcgcgt atcttcattt cctgcaattg aagatcaatc cctccggttg
    13261 gagcttcctc gtggtcggcg ttcttctgat tctggtcctt ctgggaggcg agcggctggc
    13321 ggcaagcgat aaatcctccg gatggtggag ccggcggccc ctgccgctcc gcggggcggc
    13381 cttggccctg tttgtctgcc tgatcattct tttcgccgtg gatacgggaa atgagttcat
    13441 ctacatccag ttctgagcgg ctccgatttc tggccctcct ggcctgtttc ttggcggctc
    13501 tgctcgtttt cgaccgcggt ctttccttgg ccgtccgggc gatcgagcat cgctcgtatg
    13561 gaaagagcga ctttgaacag cggttgggcc gctacttagc cggccggaat cccgcggttc
    13621 ttgtcttcgg gacttcacgg acgttcgagg gcatcattcc ggctcacttg gaaagctgcc
    13681 tgggaaagaa gattttcaag gaagcatact gggggaaggg gcctaaatac aattattatt
    13741 tctaccagct ttacaaaaag cgcgtcggcg tcccgaagct cgtgatctac ggcgtggatt
    13801 atttcatcta taacgccgaa tccaatgtcc gctggatgtc gcgcttcaac ctgggggaga
    13861 aagccgccgg attcgacgtc ttggccgggc cccttcttct gtttaagaac aagaaacaca
    13921 acgacgaatt tcttaacaac ctgatgaccg acctcggccg gacggccccg gccgctgatt
    13981 cgggcgggga cgaggacttg ttcgacaaga tcaacacgca ccccggggcg cccgccccgg
    14041 acgacaatcc aaagctgaga accgagcggc cgccccaatt tttcaagcag cgcttcccca
    14101 agccgccggg caaggagggg gactatttct tgcgccttct ggatgagctg aaagccgacg
    14161 gcgtgaaagt cgccctggtc tcgctcccgg attatatcgg ctccctgcgg accaactggc
    14221 agatgccggg ctttcaccgt catctgcagg atttgagccg gcgctacgac aacgtcaccc
    14281 tcctgatcta caacacgccg cgtgatttcg acttggacaa gccggatttg ttcctggacg
    14341 gcggttatgg aaagaccaat tctcatcttt cccagcgcgg ggccgcggtt ctgggacgga
    14401 agctttgccg ggatttggct ccgctgagcc ggtgatgccc gggccgtttc catctggcgg
    14461 agaaaggcgg gatatcgtac cgtgtcatcc gggcgttccg ggaaaaggac ggccgattga
    14521 tgtacaatat agggggatca tttcttgcca ggctggaagg atgtccatga accaagaaaa
    14581 aagaagaagc ccgcgcggcg cgcgaatgac ggaaatcgtc tacactcatg aaggccttca
    14641 tttcgaaggc cggctcagcg atctttccga aggcgggtta tatatcgaca cgatcaatcc
    14701 cgtccccgaa ggctcgatca tcgctttccg cttcgtcctc gtcgaaaagg acggcgattc
    14761 gatcatcgag ggggaaggcc gcgtcgcctg gcaacgtccg ttccagggca tggggatcgc
    14821 cttcacccgg ttgtcggatg acgtccgcga ccgcctgaaa gcccattttt cccgcgcgta
    14881 atcgattttc cgccgccgct tcgggaccgt cccgaatccg cctccagaca ccgggatcag
    14941 gccggccttc cgttgtcccg gcggcgccgg gccgccgccg cgccggcgga cgtcttacgg
    15001 agaagccggc cggcctccaa ttcaaccccg cgtttaggcc gcgtcgtttc aagcctccag
    15061 gccggtctct ccttcgggcc gggagaagcc gcctggaaat attgtctcat gcccgcttct
    15121 ttttcttgtc gccgccccag gcccgtcatc cggtcctttc ccgcatttcg gcgatgatca
    15181 aggccattga aaacgccggt tgacaagacc ggcgatcctt ggcactataa aacagatggt
    15241 caaggggagg agtctttcat gaagcgttca ttccttctat ccatcggcgc cgtgctcttt
    15301 ttggcgacgg tctcattcgg cctgaccctg ggccatccga agctgatctc aaagacgacg
    15361 gaccgcgtcc ggtggacgca atgcgccttc ggccccgacg gggtcctttg ggtggtctgg
    15421 gttcccgggg ataccaaccg cggcagcggc ggtcccgtct gggttgcgtc gtatgatgga
    15481 acggtcgtcc ggggtccgga caacgtcacc gagtcaacgg ccgttctggc caaccgccct
    15541 catatctccg tttctcccaa gggatccgtt atcgtgagct gggggatcga cgccgataaa
    15601 tcgatccatt tgcggatccg cgacgtccag gggagctggg gcgcgatcga gacggtttcc
    15661 acgggcttgg gcggggacga acccgcggct ttcatggaca aggcgggcaa catccacgtc
    15721 gtgttctccg acgaggtcgg gggttcggtc tacgcgctct ccaagatcaa cggcgcctgg
    15781 gaaaacgccg taaaattgtc cacgggtttc gggcagcacg gctccctcgc cctcgggacg
    15841 gacggggccg cccacgccgt ttgggtcgaa cggtcgggat cctccggaaa caactacgaa
    15901 aattattatg ccaagcggac ggcctcaact tcctggtcga ccggagaagc cttgacggga
    15961 caatccggaa gcgccgccca tccgtgggtg acggtcggga cgaaccatgt gccggttgtc
    16021 gcctgggagg accttcctga tccggacaag ccggggggag gatccgaaat ccgggtgatg
    16081 atggtcgggg aaacggctcg attggcgatc gccgtgggct tgaatcattt cccccgggtc
    16141 gtcgtcgacc ggaacggagc ggctcacgtg gcctgccagt cgggcagggg cgacgccggc
    16201 aacggcatcc gctatacaaa tgacgcctcg gggacgtttc aggccgtcca gacgatcgcc
    16261 ggcgtttatc cgaagctggc cgggctggcg gcggatcctt acgggaacgt cgccgcgacg
    16321 cagtcgtcgt acctgacctc gggcggaacc gacatttggg cctattccct caacctgatc
    16381 gccgccgttc ccgcgccgat cgccgcgttc actttttcgc cgtccaccgg ctatccgccc
    16441 ctggcggtga cgttccaggc cgccgcggcc tttggaccga acggcgagga ggtcaattac
    16501 gactgggtgt tcggcgacgg gggaaccgcc tccggccgaa acgtcaatca tgtttaccaa
    16561 gcttccggga cgtacaacgt ccgcttgacg gttgccgaca gcatcggccg cacggacgag
    16621 gaaatcaagc agatcgtcgt cctcagaacc gtgccgctcc cgccggtcaa cctttcagcc
    16681 tcgatcgcca tgacccggtt ctgggagaat ccggagatca cgttcaatct ctactgggcg
    16741 gccaacccgg acaacatcgc cgaacatgtc gacggctacg ttctctacat gagggagaac
    16801 gacggttctt acaaccggct gttgacggtt tcatcgtcca cgttctcggt ttcgttcgcc
    16861 ttcaccaatc tccaggtcag ccgatccttc gccgtcagca ctctcggcaa gggagggacg
    16921 gaaagcgctc cggcctattt ccaataagct cgcggaaagc gggatgaacc ggcatgaccc
    16981 gcatatcgaa gacggccggg gcgggcggag cagcggcggg aaaaccgttg cgggttcagc
    17041 tcggctgttt tgaccggtcg gccgacgggt ggctcaacac cgacatcacc ccccatatcc
    17101 ggatcacccg cgtttttgga cttccgtggc ttctcttccg tttcggcctg atcgaccggc
    17161 ttcgcttgga ccagcatcgg gccagcgtgt tttcccgggt gaaaaaattg gatttgcgaa
    17221 agacatttcc tttcgccgac ggggcggttg aggcgttctt ctgctcccac gttctcgagc
    17281 atctctacct cttccagacc cgggccgtcc tggacgaggt gcgccgatgc ctgaagccgg
    17341 gcggcttcgc ccgtttcgtc ctgccggact tggacgcggc tgtccgcctc tatcgcaagg
    17401 agaatccgga cgatttcctt caggttcttt ttcaaagaga ttccccggcg gccgaaaaga
    17461 accggcacaa atggatgtac acggcccctt cgttcatcgc tctcctccgg gagcacggct
    17521 tcgacgatgt ccgggaacga ggataccggg attcggggta tccgcccttc ctccccctgg
    17581 acaaccggcc ggaggacagc ttctacgtcg aagccgtcaa agattgaagg agagtgctca
    17641 tgccgttgcc gctccgccgc cttggacgat cggatctcct cgtgtcgccc atcgggctcg
    17701 gctgctggca attcagccag cgccggggat ggaacaagta ctggcctaag ctccccgagc
    17761 gggaaatcga gggcatcgtg gccgcctccc tggacggcgg cgtcaattgg ttcgacacgg
    17821 ccgaggccta cggagacggc gcctcggaag aggcgctttc ccgggttctc cagaaagccg
    17881 ggaaaaagcc cggggaaatc atcatcgcaa cgaagtggat gccccttctt cgaaccgcgg
    17941 ccggccttcc ccggacgatc gggaagcgcc tggaatgtct cgctccctac cccatcgatc
    18001 tttaccagat tcatcatccg ggttcgtttt cctcgacgcg gaaacagatg gaggccatgg
    18061 cttccctggt ccgggagaaa aagatccgct gcgccggcgt ttcgaatttc ggggccaaaa
    18121 gcatgcgggc ggcccaccgt ttcctggccg aggcgggcct tcccctcgtc tccaaccagg
    18181 tatcctgcgg cctgctcgac cgggccatcg aaaaaaacgg cgttctggac gcggcccggg
    18241 agctcgggat cacgatcatc gcctattccc cgttgtcgca gggcgtcctg agcgggcggt
    18301 ttcacgacaa gccggaacgt cttcaggaga tttcgggaat gagacggtac cggggatatt
    18361 accgtaagaa cgccctgcgg cgaagccggc cggtcatcga cgccttgaag gaaatcgggg
    18421 cccgccacgg ggcgacgccg gcccggacgg cccttgcttg gctggttcgt ttttacggcg
    18481 acaccgtcgt cgccattcca ggcgcgaccc gggcgtccca ggcggcttca aacgccgcgg
    18541 cgatggcctt cgatcttact tcggacgagc tggaccactt ggatcgcgtt tcccgggatt
    18601 tctccttcta gaactggaag tagagaaatt cattgctcgt gtcggcggcc aggatgatga
    18661 tgaggcaaag gaacaaggca taggccgccg cctggagcgg ggccggaacc cgccggtccc
    18721 cccagagcgc tttccggttc atgatcaaga cttcggccag gatgaagacg gccaggattg
    18781 atcccagaaa gagaatgacg ccgacgcccc gccggggaag agcccacgaa atgtgcgaca
    18841 ggtaatttcc ggccgcggcc aaggtggggg agcggaaaaa gacccaagcc agggtgacca
    18901 ggctgaagct gaagaccgtc cggaaaaatc cccaggcccg gccgctccgg cggaggcgaa
    18961 gccgccgggc ggttcttttc cggacgccct gcgtcgcccg ggaggcgacg agataaaggg
    19021 cgtggaccgc cccccagacg atgaacgtcc agttcgctcc atgccacaat ccgcagagca
    19081 gcatcgtgac cgacatcgcc gccaggtaga ggacgaactc ggttttcagg aagaggacgc
    19141 ggccggcctt gatcttccgg gacagggcgt aggagaccgg aaggaaaagg tagtcccgga
    19201 gccaggacga tagcgagatg tgccagcggc tccaaaattc ggcgatgctc cggctgaaat
    19261 atggaaaacg gaagttgtcc atggtttcaa agcccagcat ccggcccagg ccgacggcca
    19321 tgtctgaata tccggagaaa tcgcagtaaa tctggtagga ataaaaaacc aggccgatga
    19381 ccaatcccaa cccggcgtaa tcggcgggcg tctcgaagac gatgttcaca tactgggcca
    19441 tccggtcggc cacgacgatt ttcttgaaca ggccccacag caggagccgg aatccgccgg
    19501 ccgcccgtcc caggtcgaag cggtagtccc cgcggagctg ggccaggaga ggggcggccc
    19561 tctcgatggg tccggccagg atttggggga agaacgtgat gtacgccccg accaagacgg
    19621 gatttctctc ggccgagatc cggccgcggc cgacgtcgat gacgtagctc aacgccttga
    19681 atgtgtagaa cgagatgccg gccggaagga tcaagccgag gagcgctccg gcttcgccgg
    19741 gacgaaacgc ccggaggagg aaatccgaat acttaaagac gagaaggggc gcgaggagcg
    19801 cggccagggc cgtgacgaaa acgatccgcc gcccgggccg gtcctttttc tccagccaca
    19861 gggcgaagcc gaaggcggcc agggcctctc cggcgagaag aatcaggaat acgggccgcg
    19921 cccagccgta gaaaaccaag ctggccgcca ggagaatccc cggcttgatc tttcggggag
    19981 cggcatgata gacggctgcg gacgcgagaa agaacaaccc gaaggaatag ctgaggaatg
    20041 tcatgacctt gacctcgggg ggatttcttc cgcgccccgc cgggcgaaga acgggaggac
    20101 ggcctcgacg agatttcggg tgaaccggat tcgtccctcc tcggacaaat gacaattgct
    20161 ccgggggccc tttgtcgccc ggaagagcgc ggcgtcgttc agatcgaacc ggtcgggccg
    20221 attgaagtcc aggacggtga cgttcttata agatccggcc agcgtccgga tatccgactt
    20281 gtacttgtcc tgctcgaaat taatggcgtt cgtggaggcg atgtcgggga ggatgacaag
    20341 atacgccggg acgccgtcct cttccaggac ggccagcaat ttccgcagat cggcgccttc
    20401 ggctcccggc tggggccggt aggggcggcg cggcgccggc cggtccgtcc gctctccggc
    20461 gttcaatacg tcgacggcgt cggccccgtc ctccccggcg acgtcccttg cccggtccag
    20521 cttgatcacg gcgccgaaga aatcgtcgat gtccggcttc ttccggaaaa gcagcgaaat
    20581 ccggctgagg agcgaggagg cctcgttgac ggcctcggcg gggtttaagg cgtccgcccg
    20641 cagttcgcgg tccaaggccg cgagctcgtt cagcatggat tttttttcaa acatgaagta
    20701 gtcgattccg tagaagacgg cccggggctt ggcggcgttc tccttccggt gccggagata
    20761 aaagagccgg gaaaaacggg gaaatcgtcc ggcggccgcg actttgatgg tccgggcctt
    20821 gagacggtcg gacatctgga actggccgaa cgcgaaacgc gcccgcgagg acccgaagat
    20881 caagatgtct ccgtttccct cgccgtagat caccgttttg ttcctgaggg aggcgtccgc
    20941 gtcccgggag gcgtaatacg tgttggccca ggacctcaat ccgcgaagaa gaagacggtc
    21001 ggatagcgtg aacgaaacgg cgaagacaag ggtcccaaag gccacccgga agatttttgt
    21061 ccggagggaa tctcttgccg gcatggcgga ggcaagtata acgggagccc cgtcctccgt
    21121 caaaccggcc tgtccggccg gcggcgggcg ccgggcccgc ttaccatctc cagaggtagc
    21181 tgaccttgaa gaagaagcct cgcttggttt ccatgtaccg ctcgctctcg acatattcga
    21241 gcccgtccca ccggatccgc tcgaaggccg agccgtaacc cgcgtaaacg accgtccccg
    21301 ggatgtacgt gaaggatgcc aggaaatcgg ccgtccagcg cttgtaatac gtgttgtact
    21361 cggcgatggc ccggaggaag agatacttgt tgatctggaa ggtgttgtgg ctgcggacga
    21421 tcgtgtagtc gtagatcttg accttgtccg accggcgggc gaagtcacgg tagacgaggt
    21481 cgagagcgaa ggtcagctgg tcggtcggct ggaaggacag gccggcgctg gcttggttcg
    21541 agtctccctg gtaaggatcg tcggggtcgt aataaatgga tccccggttt cgataaaaca
    21601 ggttgagata aacctgtttg gtaagctggc tgtagaattg aaagccgtat ccgctccgcc
    21661 cgaagcggcg ggcctcgtac acctcgttgg ccagcatccc ctcgaagcgg acctgggtcg
    21721 accggggaag gaagaaccgg aggcagaaca tgttgaacgt ctcgaacatg ttcgaggtcg
    21781 tgtcttggag atggtaggac cagtagaagg tgtcatacct ctggatgatc ttggacttcg
    21841 ggtagaacat gaggattccg caggccgcca agcgccgcag ccccgtccgc tcgaggaagc
    21901 ccgtgtccac ctggaagttg cgggaaatgt cctggtatcc gaaatcgagg tagtaattcc
    21961 gcgtgccctg gttgtaacga aggccgaggg cgtggccgcc ggccgtttct cccccgtccg
    22021 gggccttgga aaacgacccg aagagatgga agtcggcgaa actgatcggg ctcagccgga
    22081 accggccgtc cgcgccgatg acccggttga atccgccggt cacgtcccgg gcggtatagt
    22141 atccgccgat gaaggaatcg tccttcaaag cgtgcttgag ccggaagacg gagaaatagg
    22201 gatgctcatc gtcggctccg ggctggttgt cgcgggcgaa gatggcggcg atggaattgg
    22261 ccgccccgat tttcccggac agcttgaagc cgtatgtcgg atcgatgatc gtccgggtgt
    22321 gaagaagggc atagaggggc gcgtcctcgg tgtttccggc gaccgagaag atctcgttgc
    22381 cctcgaggaa gaacggcctc ttctcggggt agaagagggc ataccggagg ttgatgtcga
    22441 cctggccggc gtccgactcg acctggctga agtcgggatt gagggccgcg tccaaggtca
    22501 ggtcggtcgt caagccgacc ttggccgtca agctgacgtc ggtcgccctc tcctctcttt
    22561 tcatcgttcc ctcgtcgacg gctcttcggt cggaatgcgt tacggccggg aggatctcga
    22621 cgacgcgttt gaaccgccac ccggtgacgg cgatcggctg ggtctgagcg agaaagctgc
    22681 tcttgtcggg gtagacgggc ggggcgctgg ccatttccga ggttcggacg atctggcgga
    22741 agtaggccag ccggatggtg atcgtcttgc ccgaaggaaa ccggatgctc tgcagcggga
    22801 cgcggtattc gacgatatag ccctggtcgt cgagccggcc cttgctgtcc cagacgaaat
    22861 cttgttcggc ggtcaagttg ccgtcggttg tcatgatgcc gtcgccctgg acgccgaggg
    22921 gattgaccag gaaagaatag ccgttctgca tcgtgttgta ggtgtcgacc acgatcccga
    22981 cgaaatcgtc ggcgtacatg tcgtcccgct tggccagggt ggccttgatc ttggacggtt
    23041 ccgtgtcata gcaccggaag gcgaagtaga ggttgtcggc atcgtacagg aaatacgcct
    23101 cggtcttctg gctgggctcc ttgccgtagt ccggctgaaa cgtcttgaat tccgacatgg
    23161 cgtaagcccc gtcccattcg ccgggatcga gcttgccgtc gatgacgggg ggcttggccg
    23221 tggtggccag ctcgagagtc gtcaaggcgg aaagaaaaac gggaagaaaa acagcggtca
    23281 aggcaaccgc ggtgatgagt tttctcaagc gggtgttcat gtcgaaatcc tccgtaaagc
    23341 aaccgcgcgg caatcgtcgt tgaccgcccg ttcattcgga tatacggtgc ggagggaaaa
    23401 aaggttcatg tcaacctttt cctccgcccg agagtctttc cttctgtgag cttggaaaat
    23461 aacatgagag gatgtcatga ataatcgttt cttccgcatc ggagccgctt tattgatcct
    23521 cggcgccgtc ggacaggttc tggccggcgc ggccggcggt cccggcgtca agaaatgcgc
    23581 ggcgatccgg atcggcgaaa gcgtcccggt catcgacggc cggctcgtcg attccgcctg
    23641 ggagaacgga tcctgggaaa gcggcttcgt ccaaagagat ccctacgagg ggcgggaacc
    23701 tacggaacaa acgtccttca agattcttta cgatgataaa aatgtttacg tcggcatccg
    23761 ggcccatgat acccgccccg acttgatcga aaaacgcctc ggccgccggg atcaagccgg
    23821 cggggacgtg gttctggtcg ctttcgacag tctccatgac cacttgaccg cttatgtctt
    23881 ctcggtcaac gccgccggag tcaaggccga ccagatcgtg gcaaacaacg gcctttccgc
    23941 cggcggcggg gaggaagaca tgagctggga tcccatttgg gacgcggctg tttcctcgga
    24001 tgatcaaggc tggtcggccg aaatgaggat tcctctctcc cagcttcggt tcggggccaa
    24061 ggacgagcag gaatgggggc tccaggtcct tcggaccctg tttcggacca acgaaacttc
    24121 cgagtggcag ctcattcccc gaaaagcccc cggctgggtt cacctcttcg gcgagctggg
    24181 cgggctggcg ggcctggccg cgccgcacca agtcgaggtc atgccttacg cggtcggaag
    24241 cctgatgtcc tcccgccccg tcccggggaa tcccttcgcc accgggcgga accggaggct
    24301 gcggagcggc ttggacggca aggtcggggt gacgagcgac ctgacgttga acctgaccat
    24361 caatcccgat ttcgggcagg tcgaggctga tccctccgtg gtcaatctga cggcgctcga
    24421 gacgttttta caggagaagc gtccgttttt catcgagggc cggaatcttt tcgatttccg
    24481 gctcacgagc ggcgacggcg atttttccta cgacaacctt ttctattccc gccgggtagg
    24541 ccgctcaccc caggtcatgc ccggggggcc tggtttcgtc gacatgccgg cggccgcgac
    24601 gatcttgggc gcgttcaagc tgacggggaa aaccaagtcg ggattttccc tcggcctcat
    24661 ggaatcggtc acgtcgcggg aatcggcctc ggtttttcac gatgaggttt acaccgatgt
    24721 ggccgtggag cccctgacca attatttcgc cgcccgggtt caaaaggact ggaacagcgg
    24781 ggcgacggtc ctcgggggga tgatcacggc cgtcaaccgg gaggcggccg gcgaagcgtt
    24841 cgagactctt caccgggccg cttattccgg agggattgat ttttcccaca gctggggaaa
    24901 ccggaattat tacctcagct tcaaggctgt gacgagccat gtccggggaa cggccgaggc
    24961 gatcctggcg acccagctct ctcccctccg caatttccag cgtccggacg cgggatatgt
    25021 cactctcgat cccaggcgga cgtccttgac cggcacgggg ggcaatttcg agatcggcaa
    25081 gcagggcggg ggaaactgga cgtatgccgc cggcgtgacg tggcgctcgc ccgggctgga
    25141 gctcaacgac gccggctatc tccggcagac ggacatgatc atggcgttcg tttgggccgg
    25201 ataccgggtc acggagccct tcgggccgtt ccgccgagtc aatgtcaact tcaacggttg
    25261 ggcgggcttc aacttcggcg gggagaccat tttcgagggc ggcaacgtca acctcaacct
    25321 gaccttgaaa aatgaatggg aggccggttt cggctacaat ccccaagggc cctccctctc
    25381 cgcggcggct ctccgcggcg gaccgactct gtatttgccg ggggggaacc acttctgggg
    25441 gtatgtcggg accgattcgc gcaggaaggt ccggttctcc ctttttggaa gcgggggccg
    25501 gcgggggccg gccgagatcg tttcttggag catgtccccg gagttgaccg tcatccccag
    25561 ctccgccttc caagtctccc tcgctccgtc ctattcccgg aacgacaacg tcctccaata
    25621 cgcgggaacg cggacggccg gcggggagcc gcgatacgtc ttcgggacga tcagccagga
    25681 aacgctggcc ctgaccttga ggatgaccta ctgcctcacg ccggacttga cgatccaata
    25741 ttacgggatg ccgttcgttt cggccggcgc ctaccgcgat ttcaagacga tcctgaattc
    25801 gcggtcccgg atccccgagg agcggttccg cttgctgggc gaggcggccg tctatgatcc
    25861 gtccgcccgg caataccggg tggacgagaa cggggacgga accgtggatt acgcgttcgg
    25921 aaatccggac ttcaatgtcc gtgaattccg ctccaacctc gtcctccgct gggaatacac
    25981 tcccggctcg gccctctacg tcgtttggtc gcaggggagg acgggatttc ttcccgaagg
    26041 ctcattcgat tacggccggg atttaggggg ccttttcgac acccacccgg ataatgtctt
    26101 cctgatcaaa ttttcctatt gcttccagct ttgagacggc cgtcccggag ggaaggattc
    26161 ccttcccgcc gggatcaggg cgtttccttt ttctttccgt ccctcttgtg ttcgaacagg
    26221 tagtatacga ccggaacgac gaccagggtg atcagggtgg agctggtcaa accgccgacg
    26281 acggcccggg ccagcggggc ttgggcttcc gcgccctcgc ccagggccag ggccaggggg
    26341 atcatggcga tgatggtcgt cgcggccgtc atcaggatcg gccgaaggcg gcgatgaccg
    26401 gcctcgatga ccgcgtcccg gaggcccatc ccgtcccggc gtcgaagcag gttcgtgtag
    26461 tcgaccagga ggatggcgtt gttgacgacg atcccgccga gcatgatgca gccgatataa
    26521 gattgcatgt tgaaggtcgt cccggtgatc cagagcatca ggacgacgcc gatgaccgcc
    26581 agggggacgg aaaacatgac gatgaacgga tcgcggagcg actcgtagag catggccatg
    26641 atcatgtaga cgagaaggag ggccagggtg atgctcaaga ggagttcgcg gaaggccttc
    26701 tgctgttcct cgtagtcgcc ggtgaagttg atcgcgaaat tcgagggcgt cgggatcgtg
    26761 cgcaaggcgc gccgggcgtc ggccaggacc gagcccatgt cccggccggc gatgtccccc
    26821 gtgacggtga cgacccgttc ccgatcccgc cgctcgattc ggatcggccc ccggcggggc
    26881 tggacccggg cgacgttgcg gagactgacg gcccgaccct cggaattggt caaggtcagg
    26941 tcgaggatct cgtcgatgga caggaactcg gagtctttca ccttgaccag gacccggtac
    27001 tcgcgcccgc cctcgcggaa cgtcgtggcc tgggtgccca tgaggatggt ctggatcgtc
    27061 tgcccgatct gggtgatgga cagcttctgg tcggccgccg ccttgcggtc gacgatgaac
    27121 agctcttccg gattccccag gtcccggctg atccgggtgt cggtcacccc ggggagggct
    27181 tcaaccttct tctggacctg ccgggccaag tcttcggcga cgagaagatc atagccgcgg
    27241 atctcgatct ggaggcgggc catcgagccg cctccgcgca tcatcatgcc ttgctggccg
    27301 ccggtggccc gggtccggat gatgacgccc gggatatcgc tcaggtcgcg gcggaggtcg
    27361 gccgcgaccg tctcgctgga gcgcgagcgc ttgtttttcg gaacgagctt gacggtgagg
    27421 ttgcccgtgt tcgaaccgct ccgccagccc ccgcctccgg cgttttcctc gaggctgatc
    27481 atttccggaa ccgtccgccg gacgacggcc gacacttgtt tgattttatc gtccatgagg
    27541 ccgaggcgcg tgccgacttc catctcgacg ctcacccgga cctcgccctc gtccgtcgcc
    27601 ggcatgaact cgttgcccac ggcccggatg agcagcaggc tgacgatgag gagaccgccg
    27661 gcgattccca gaatcacggc cttgtgatca agggcgtaga cgagcaggcg gcggtaggcc
    27721 ttctccagct tggccacgac gccccgggtc gaccggacga aggcgacgtg gaatcccggg
    27781 gccgagcccg gctcgatttt ctccacacgg agatagcggc tgcagagcat cggaaccaag
    27841 gtcagggcga cgaagagaga gacgatcagg gagaagctga tcacgtaagc cagctgcttg
    27901 aacatgatcc cggacatccc gcggatgaaa accatgggca tgaagaccga aacggtggtc
    27961 agcgtgctgg cgatgatcgc cgagatgacc tcttggcttc ccaggacgga ggcttggcgg
    28021 atgggcaggc cgccttcgtg caggcggtag atgttttcca ggacgacaat gccgttgtcg
    28081 accatcatgc cgacccccaa ggccaggccg cccagggtca tgatgttcaa ggtgaagccg
    28141 ccgaagtaaa tcagggcgaa cgtcccgatg atggaaatcg ggatggccgc cccgatgacc
    28201 agggtgctcc ggatattgcc caggaagaac agcaggacga agacggccag cattccgccg
    28261 tagagagccg aggagccgac gttgttgatc gcccgccgga tatactgggc gctgtcgctg
    28321 acgacgatca gctcgatctg gggcaggtct tcccggatct tggccaactc ggccttgatg
    28381 ccctcggcca cggcgacggt gttcttgccg gactgcttgt tgatggcgat ccgcgtgccg
    28441 ggcttgtcgt tgatccggac gacccgggtg atcttgcgga aggtgtcctc gatggaggcg
    28501 atgtccctga gcaggatggg ggaaccgttc cgaaaagcca cgacggtgtt ccggatctgg
    28561 tccaggtcgg tgaacgcccc cgggatccgg agaagaattt catagttgcc tcgctcgatc
    28621 tggccgatgg gcaggtcgag gttttccctg cggatgacgg acgtgatcgt gtccatcggg
    28681 atccccaggg ccttgatctt atcggcctgg aggttgactt ggatctcccg ctcgcgtcct
    28741 ccccagacgt cgaccgaggc gacgccgggg acgcgctcga gccggtagac gatctgctcg
    28801 tcgatgaggc gccgggttcg gatcgggtcg agattgctga ggacgccgac catgaggatc
    28861 gggaactggg ccggatcgaa ttttcggagc gtcggccggt ccacgtcctc gggcaggcgc
    28921 ccgacgaccc ggtccagccg ctcccggatg tcgtcggcgg ccgcgtccaa gtccgtcccc
    28981 caatcgaagg tgacccgcac gacgctgctg ccttccgacg aggtggaaaa aacctcctcc
    29041 actcccggaa ccgcgctcat cgcctcctcg atgggccggg tgatgagttc ctcgatctcc
    29101 tcgggcccgg tgttttcgta ggagcagacg acgttgacgg tcggataggt gatctcgggc
    29161 atcaggtcga cgggcagccg gttgagggcg acgaagccga cgacgacgat gaccagggtg
    29221 atcatcacgg tgaagaccgg ccgccggatg gagaattcgg aaatggtcat cgcgttgctc
    29281 cgtggggctt gccggccgga tccgcggccg gcggcccgcc cccgtttgcc tgcggcagga
    29341 tcaggccgac cccgtcctcc aggagatgct ggccaagggt gacgacctgc ccggtcagcc
    29401 cggccggttc gacgacctcc gcccattccc cggaaacgat cccggtcctc accgggacga
    29461 agaccgcctt cctcgttttt aaatcggcca gaaaaacgcc ggccgctccg gcgcgctgaa
    29521 cgaccgcgct gagcgggacg atcgtcgcct ccgcccgccg gtcgtactcg atttcggcgg
    29581 tgataaacat ccccggcttg aggaggccgt tcggattctc gatctcgatt tcgacgcggg
    29641 cctgccggga cgtttcgttc agcaggggcg acatccgggc gacccgcccg ataaaggccg
    29701 tgtcggggaa cgccgtgctc aggatgttgg tttcctggcc gggctggagg cggtaggaat
    29761 cccggtcggt gacgaagatc acggccgtga tggggtggag ctcgatgatc gacaggatcg
    29821 cgccgttggc cgggagcatc gatccctcgg tgacaaagcg ttcgccgacg taccggaccg
    29881 cgcttccccg ctcccaggcg gcctggatcc gggtataaga cagccggagg cgggccgttt
    29941 ccagggccgc ggtccggttg gcttcctggg cctgggcgac attggaccgg gcttcctggg
    30001 cttcatactg ggcccgggcg gcgtcgagct gcgaatccga ttggatgccc ttggcgtgga
    30061 gcgacttggc tctttccaag tcccgcttga ccatctccag ggagcttttc gcctcggcca
    30121 ggttggcttg ggcgacgcgg agatcggcct cggcctgggc gacttgctgg cgatactcat
    30181 cgtcctcgag aacggcgacc agctggccct gccggacggt gtcgccgata tcgaccagaa
    30241 gctgcttgat ccggcccgag attttgggag cgacggtaaa gctcgtcttg gggatcaggg
    30301 tgcccgagaa caagcccacg tcgcggatcg gcccgcggcg gatcgcggcg gtttcgacgg
    30361 cgaccatgac gttccggggc ccgttctgcg ggcctttctt ggcgaaaaag acttgataag
    30421 cgccccaacc ggccgcgatc aggacggcgg ccaaggcgat tttcaccggg agcttggccc
    30481 ggcggaggat ttgaaaaaac gattgtttag gcatggcgga tctcgcttgg gaattggtgt
    30541 cgtttgacgg tttatcggtc atgggaggaa cagtcatctc tccgccggcc cgccggattc
    30601 cccgggaagc gggacgcgct ccatctccaa gcctttgcct tgggcccggt agaggccggc
    30661 cagcgaaaca ttgtaatcga cgacggcctt cagttccatg gtcagggcgt tggccaggtc
    30721 ccgctggtat tgaaggatga aataattggt gctcagcccg actttgagct tctcctcctc
    30781 ggcctcgagc ttcttggccg cgttttcccg ggcgacccgg taggcctgaa tccggaggta
    30841 attggtctcg accgcccgga cggcggtccg gatatcaagg aaaatctgtt gttcctggtt
    30901 tttcatccgg agctgggcct gctcgagagc caagtcggct tgggccacga acgcccggga
    30961 aaagaccgtg ctcaggggga tgttcaagct caatccgaga gaccagttct tgtacctgaa
    31021 atccatggca tccttcaggg cttgaccgcc gccggccggg acgaccccga tgatcttgcc
    31081 cgataaggcg ttgttgttct catagatgat ttgcgttccc gagatgcccg ggctccacag
    31141 cgaagcctgc aggcgcagat cgggcgccgt ctggtttcgg gcgtagctga ggttgaaccg
    31201 gctcgtttcc atgccgaccc gggccgcctg gagatcggga cggttctcat aagcttcgcg
    31261 gagagcccgg tcgagatcca cgtccggctt ggaaaccgac ggcgaatcag tcgggatgat
    31321 gtggagggag gcggcccgcg tattctccgc ctcgaggttc atcagggttt tcaagaggtc
    31381 ctcgctgttg cggaccatgg cctccgcctc gagaatatcc gcttcccggg tggagacgtc
    31441 ggcttgggcc gtcaagattt cgatgggggg cagggtgccg gcttcgatct cggctttatt
    31501 tttttccagc agctcccggg ccaatttcaa ggattgccgc cggacattca aattttcccg
    31561 gctatacacg agattccaat aagcgctctt cgcccggtag acgtattcct ccatcgtctc
    31621 gctgaggttg tattcggaca tgtcgcggtt gttccgggcc aggatgaact cccggcggct
    31681 cattttccag ccgaaatcct tgagaagagg ctgggaaaaa ttgaaccgga gggtgctgcc
    31741 gaagcgcggg ttgatggttt ggaattgccg ggtggtatcc gatttatagc cgatgaggct
    31801 catggagagg gttcccccgg tcggaagaac ctgggaaaat tgggaggagt aatcattttg
    31861 cagcgtggag acggccgccc cggcggcgtc caggaatgaa tacgaggcgg agatattgtc
    31921 ctgccggctg taggaaaggg ccagcgaggg gatgaatttc tctccggcca aggcgaccgc
    31981 ggcctcggcg atttgcggcg tgagaacctc ggccgccagg ccgaggttgt tgttcagggt
    32041 tttctggatg cattccggca aggacaaatt cagattctcc ccttcctgga ccgccggggc
    32101 cggcagggag aggaccgcca gaagcagggg gatggctgcg cgggtagggc gtttcataat
    32161 ccgttaagac ctccaagatg aatccggccc gaaggcgagg taaaaaccaa ccttcatatt
    32221 ttagacgaaa agagggccgt tctctttcct tttttttctt tttggataac tgattgattt
    32281 aacgtgagtc ttgcggaaaa caaggctttt cggaaaaggg agaaaaagcg gctcttgacc
    32341 gggaacaaac tcttttccgg tctttttgga ttataatgac aacatgagca ccgcagaggc
    32401 tggagtgttg ctcctggcct tggccggctt gtttttttcc ggcggggggc cggccccggg
    32461 ccgggacgag aacaccgtct tcgcccggcg ccgccaagcc atggtcgccg ggcagctcgc
    32521 ctcccgagac atccgggaca agcgcgtgct tgaggcgatg ggccgggtgc cgcggcacct
    32581 cttcattccg ccggagtccc gggagctggc ctacgaggat tttcccctgc cgatcgaaga
    32641 aggccagacg atctcccagc cgtatatcgt cgccctgatg acccagtgcc tggagctcag
    32701 gggcggcgag cggatcttgg aaatcggaac cggctcgggg taccaggccg ccgttctctc
    32761 ggtcctggcc gcgaaggtct ataccatcga gtatcacgcc tccttggccg ggaccgcggc
    32821 cgcccggctc gccgagctgg ggtatgccaa cgtcgaggtc cgctccggcg acggattttt
    32881 cggctggccg gaagccgctc cgttcgacgg catcatcgtc acctgcgcgg cccggcgcct
    32941 gcccaaaccc ctgttcgacc agctgaccga aggaggccgg ttggtgatcc ccttgggccg
    33001 ggccgccgat gtccagcggc tggtccgggt gcgcaaggtc aaggaaaagc cggtcgagga
    33061 ggaaatcacc ctggtccggt tcgtcccgat gctcggcgcc gataaaaaca agagataaag
    33121 aacgatcatc cccgcaacct ttagccgggg gaattcgtaa tgaaaagtga gcgtatcctt
    33181 ccatatccca aaggagagat catgtccaag aagtcgtgga tgttcgcttt gttgttcagc
    33241 gtcatcgcct tgtccgtcat cttcgccgag acgacggaag cccgtctcct ccggttcccg
    33301 gccgcgtccg gggaagcgat tgtcttcagc tccggcgggg atctttacac cgttccgctg
    33361 gaggggggct tggcccgcaa gttgacaagc catccgggtt acgagatgtt cccccgtttt
    33421 tccccggatg gaaagaccat cgcgttcacc ggcgaatatg acggaaaccg cgaagtctac
    33481 gtcatgccgg cgggcggcgg catcccgcgc cgcctcacct atacggcgac cctcggccgc
    33541 gacgacgtgt ccgaccgcat gggcccgaac aacatcgtta tgggctggcg gcccgacggc
    33601 cggagcatcg ttttccgcag ccggatgagg gaatgggacg atttcatcgg acagctctat
    33661 gtcgttccgg ccgagggcgg cacccccgtc gcgcttcccc tgccgcgcgg cggcttctgt
    33721 tccttttccc cggacgactc gaagctggcc tacaaccgcg tcttccgcga attccggacc
    33781 tggaagcggt acaggggcgg gatgaccgac gacatctggg tttatgattt cgagacgaaa
    33841 aagaccgaaa acatctcgac caacgaggct ggggatatct tcccgatgtg gagcggagag
    33901 acgatttact tcctttccga ccgcgacgag aacaagcgga tgaatctcta tgcttacgac
    33961 gtgaagacca aagaaacgcg ggccttgacg cgcttcgccg atttcgacat caagttcccg
    34021 tcgctcggcc cgacggcgat cgtcttcgag aacggcggat tcatctaccg cttcgacctg
    34081 gcctcgcaaa aagcggcccg ggttccgatt ttcatcgagg atgaccaggt ttcggcccgg
    34141 ggacggatcg ccaatgtcgc ctcgcgcgtc acgaactacg aaatctcccc cgacggcaag
    34201 cgggccctct tcggcgcccg cggcgagatc ttcaccgtcc cggccaagta cggaaacacg
    34261 cgaaacctca ccaacacgtc ggggatccac gagcggaatt ccaagtggtc gccggacggc
    34321 cggtggatcg cctacatttc cgacgcctcg ggcgaggacg agatctacct ccggccccag
    34381 gacggatccg gcgaggccga gccgctgacc ttcaaggccg acacctacaa gtacggattc
    34441 ctgtggtcgc ccgacgggaa gaagatcgcc tggacggaca agctgttccg gctccagtac
    34501 gtcgacacgg cgaccaagca ggtgactgtg gtcgaccggg ccgcggtcgg cgagatggga
    34561 ccgttcacct ggtcgcccga cagccgctgg atcgccttta ccaagccgga agccgaggga
    34621 atgccgaagg tttatcttta cggcctcgac ggcgggaaga cggccgcggt gacggacggc
    34681 tggtactcga gctatgagcc ggcgttcagc ggcgacggga aatacctctt ttttgtttcc
    34741 gaccgggatt tcaatccgca actaagcgag atcgagttca attattccta ttcctcgatg
    34801 gctcggatct attgccttcc cctggccgcc gacaccccgt ctcccttcgc cccgaagagc
    34861 gacgaggtcg aaatcaagaa ggccgcggag gcggaaagcc cgaagagcgg agccaaggga
    34921 aagccggccg agccgaagaa gtccgagccg gaaaagccag ccggcgtcac ggtcaaggtc
    34981 gacctcgaag gcctgaagga ccgcctgatc ggtctgcccg tcgaggcggc caattacggc
    35041 ggcctggcct ccgtcgggga taaggttttc tacttgaaat tcagccgggg cccgggcggc
    35101 ggaccagcct tcaaatccta cagcctctcc gaccgcaagg aaagcgacct gggccagatc
    35161 gacggttatg agatctcggc cgaccagaag aagatgcttg tctcccaggg ccggtcttac
    35221 gccatcatcg acctgcccca ggggccggtc aaaatcgagg accgactgga tctctccggc
    35281 atggacatgg ccctcgacct caaggcggaa tgggagcaca ttttcctgga gagctggcgc
    35341 cagatgaagt acttctttta tgcccccaat atgcacgggc aagattggga cgccctgaga
    35401 gagaaatacc tgcccttggc ccaggcggcc ggctgccggg ccgacctgac ctatgtcatc
    35461 ggcgagctca tcggcgagct cagcgccggc catacctacg tcggcggcgg cgagatgccc
    35521 gatctgccca aggtcaaggt cggccagctg ggcgcggttc tcgtcaagga cgcctcggga
    35581 tatttccggg tcgacaagat cctgcgaggg gagaactggg accgctcgct ccgctctccg
    35641 ctgaccgagc ccggcgtcga cgtcaaggac ggggattaca tcctggcggt cgacgggcgg
    35701 ccgaccaagg agatgaccga catctacgcc tcccttctga acaccgtcgg caggcaagtc
    35761 accctcacag tcaacgggaa accggaggac aagggcggcc gccgggtggt tgtggtaccc
    35821 gtcgccgacg agcagaagct tgtctattac acctgggtcc gggagaacat cgccaaggtc
    35881 gacaaggcga ccggcggcaa ggtcggctac gtccacatcc cgaacatggg gctggacgga
    35941 ttgaacgaat tcgtcaagta ttactatccc cagcttcgca agaaagcttt gatcatcgat
    36001 gtccgcggca acggcggagg cttcgtttcc cctctggttt tcgaacggct gcggcgggaa
    36061 atggcgatga tcgaaatggg ccgcgacacg atcgcccggc ctgatcccgg cgacgtcctg
    36121 gtcggaccca aagtctgcct tttgaacgaa ttcagcgcct ccgacggcga catcttcccc
    36181 taccggttca aaaaagcggg catgggcaag ctgatcggaa agcgttcctg gggcggcgtc
    36241 gtcggaatcc gggggaccct gccgatcttg gacggcggcg tcctgaacag gccggaattc
    36301 gccccttata atatggaggg caagtggatc atcgagggcg tcggcgtgga gcccgacatc
    36361 gtcgtcgaca atgacccggc ccgcgaatat gccgggatcg acgaccagct gaacaaagcc
    36421 gtcgaggtta ttctcgagga gctcaagacc aaggaagtca cgctgcccgg gattccgccc
    36481 taccccatca agaaatagcg gcccgccgtt ccgcggatct cgtttccgcc ggagcccggc
    36541 tcttcggagc cgggttccgg cggctttttc gaagccggcc cgacagaggc ggcgatcctg
    36601 gccggcgcct cgcgaggcac ggcggtggga ggaaattttc tcgtcagacg gggactcctc
    36661 tccctcggaa aggagctgaa cccgctccgt tccaaggcgg aaatccaagg ggggaggctc
    36721 ccgcgtccgg ccttccggaa cggttcttgg aagccggagc gcggttggat ggcagccgat
    36781 cagcccggcc gattcaggcg gagcttctcg aaagaacgag ctcatcaccg ggactcgttc
    36841 cgccggttga cttgccgtcc gcccgatcat atgatgctgt gcgtgaagga tatttccggc
    36901 tcgacagact taaccggaac agacctcaag cattaccgca tcgaggcgtt catcggccag
    36961 gggggcatgg gcgtcgttta ccgcggccgg gacacccggc tcaatcgtcc ggtggcgatc
    37021 aagctcctga gtcccgccct ggtcgccagc gccgaacggc ggaaccggtt cgtccaagaa
    37081 gcccgggccg cggccgccct cagtcatccg gccatcgctc aagtctatga catcgacgag
    37141 gtcgacggtt gtctgttcat cgtcatggag tacatcgacg gccggtccgt cggccggctg
    37201 atcgccgatc gggagttgga tctcttgagc tccgtcgaga tcgccttcca ggtggtcgaa
    37261 ggactggaaa aggcccacga ggccgggatc cttcaccggg acatcaagtc ggacaacatc
    37321 atggtcacgc gcgacggccg ggctaagctg cttgacttcg ggctggccaa gctgatggaa
    37381 ccggctccgg ccgcatccgc tccgccgtcc gatcctctcc ggaccttgac caaggacgcc
    37441 ggccggacct tgcccggcct ggtgatggga acggtcccct acatgagccc ggagcaggcc
    37501 cgggggcggg acctcgacgt ccggagcgac ttgttttctc tcggcgtcgt tctctatgaa
    37561 atggtcacgg gcgagctccc gttcaagggc gaaacgccgc tggacacgat gcacgccatc
    37621 gcctacgagg aagcccggcc ggtgacggtc gtccgccgca atcttccgcc gcaggtccac
    37681 cgcatcgtct cccgctgcct ccggaaacgg ccggaagacc gctatccgga cgccgagacc
    37741 ttggcctccg atctgaagcg gttcaaactc gacctggaat cgggaagccg gacggacctt
    37801 cggccgattg aacgcttgca ggcctggttt gaccggatga taagctccgt gccgctggga
    37861 gccaagggcg tttggatcgt cgccggggtc gtcgttttag cggtcctcct ccttttcacg
    37921 agcttcaatt ggggaagcct gatcgccccg gccgttatcg gcgttttcct ttaccgggga
    37981 attcggaaca ggaaaaagcg gctgctggcg gacttcgcca aaaaagtctc ccggatgccc
    38041 tccgtcgagg ccgtcatttt ccacgacggc aagatgacga cggtcgtgaa ggacgctcca
    38101 gcctcggcct ttgtccggat caccggcctc atcgaggaga tgaacggaaa gctgtttttc
    38161 ggcaagcccg tggcggccga gatcgcgagc gatctccccg cggaggaatt tcaagctctc
    38221 atccgccggc tcggcgtgct gtatgtccgg acggagcggc ccggagaacc gcccgcgggc
    38281 aagcaaggaa aaaacaaaga ccattctttt tcgggaatga aataagcgga aagagaggat
    38341 gagcaaaagg acgagatgaa tccggatccg cggacgatcg gcgacgttct cctccgcaga
    38401 aacgccgaga actcggcccg gctcgatttc acccgggtcg atgcctatcc ggagaacatc
    38461 ctcaaccgta tcctctggca cgcggtccgg ggaagcggcg cgccttatcc ggcttgggcg
    38521 gtctcggcgg agcgggaaga cgggaagaag ggggctccga ccagatgagg agtgtttttc
    38581 ctttgttttt aagtataata ataaaagggc cggccgccgg gccggagccg gcggaaaaaa
    38641 gaatgggagg gacaggatga aaagggaatt cacagccgcg tttctgttga cggtgatggc
    38701 cgtttcttgg gccgccgccg ctccgccggc ctccgatatg gatatttgga aggaattcct
    38761 tcagctgctc aggtcgggaa ccctgacggt cgactggatt catccggatg atccccggac
    38821 ccgggaatcg cggctgacga atctgctgga ttggaccaag gacgaagatt ggtcggaatg
    38881 gcgggccact ccaaaggtcg tccgtcaaga caatctcgtt tcgttcatca tcgaggtggg
    38941 gaaaaagcgc cgagctccct ggacgtatgt cttttgtttc accgtcgaag ggggccgctg
    39001 gtattaccga ttcgtcgagg gcgttttcct ccggctggat caagtcgtgt cccttccggc
    39061 cgacgcctcc tcgttccccg acctgtccga ggaacggaag aactggatgc ggcaggaaat
    39121 ctattggacg aagatcgtcg ggctgttcac cgagatgacc cgcctcaagg ggacggaata
    39181 cgccttggga tttctccggg acggggcgga atacgtccag gcggcgacgt cctggattcc
    39241 ctattttccc gcatcccggg cttttatcct ttacctctgc tgggagcagg cgaggatgca
    39301 gggaaacaag gtcgttctgg aaaagctgac cgatcgcgaa gcggtcgtcc ggttcgacga
    39361 tcatcaattc ttcgcgcttt acatgcagac atcccatctc cgggagcaaa tcgccttggg
    39421 ggattacatg ggcatcttcg aggcgatctg gcaggatcgg gcccgggccg cgggctggaa
    39481 tctccagatc gacggacaag gcccgacgat ttatctgcgc ttcagccgtt agaggcgcgg
    39541 ggggccggcc gaaggactcg tcttttccat tataagcgat cgagtcggcc gcctctcttg
    39601 ggagccggga tcgtccttga tccccaaatc cgcgatcgtg atcgcaactc cgggcggctt
    39661 gaaatacggc ccggggcgtc ataagatagg ccgcgaccgg aacgtttgaa aaaaaccgct
    39721 gtttcccgga ggaccgtgat gaacgcacgc cggctgaacg ccctgatcct ggccgccgct
    39781 cttctttccc cggctttgtc cgccgcgacc cgcgatggcc ggaatttcga ttgttacacg
    39841 attgttgtcg ggaaggccgc ttcggccgac gggtcggtcc tcctggccca taacgaggac
    39901 gattggggcg acaatatcgt caatgtccgc cggattccgc cgggcgatta cggccgggag
    39961 aggaatgtcg ccctcggccg gggagcccat taccggacgg acggccggac ggcgggattc
    40021 ctctggatcg aggtggcccc ccaggaatac tccgacagct tcgtcaacgc gcatggagtc
    40081 gtcatcgttt cggacagctg tccgacccgg gagacggaga gcgatctgac cgacggcggc
    40141 atcggttaca tgctgcggag gatcgtcgcc gaaaaggccc ggacggctcg ggaagcagtg
    40201 gccctggccg gcgcgcttat cgaacaattc ggctaccggg attccggtcg cacctatgcc
    40261 gtcgccgacg ggaacgaggc ctggctgttg gccgccgtcc ggggacgccg ctgggtcgcc
    40321 cagcgggttc ccgacgacga ggtcgcgatc cttcccaacc acatgacgat ccgccgggtg
    40381 gacttgggcg attccgtccg cttccagggc tccgccgatc ttgtgtctta tgccgaagcc
    40441 cgcggctggt acgatccgga gaaggacggg ccgtttgatt ttaaaaaagc ctacacccgt
    40501 ccctcgaccg accttctggc cgaccacaat acccttcgcc actggcgggg gcttgagctg
    40561 ctgaccggcc gggaatggcc catcggcgag aattatccgt tttccgtgaa gccggcggaa
    40621 aaagtcaccg tcgagcggct gatggcgatt ctccgcgatc accacgaagg cacgccttac
    40681 gacgccacgg atggatacaa gaccggttcc ccgaaccgcg cgaaatttcg gacgatctgc
    40741 acggcgacga cggtcacgag cctgatcgcc tccctcgacg cctcccgccc gggaccgctc
    40801 gcggcttccc tttggctgac gctgggaaaa ccggatacga cggtcttcct ccccttgtac
    40861 ggcgccggcg gagccctccc ggccggcttc ggcctgggtc cccaaacgca tgacgacgag
    40921 gccttctacc ggcagcattt cgaggaggcc gggttcaagg cggctcgagg gatgctcctc
    40981 aatacccgcg tcctcgccgt ggaaaaagcc gtggaggctg attacggggc caagatcgag
    41041 gccgtgcgga agggaatcga gattctcgag cgctctttcc ttgacggccg gagcgcgttc
    41101 gaagccgccc tctccgccca atacgcggtc aacaaggccg aagccctaaa gagcgtcgac
    41161 gattacgtcg cggccgcgtt cgaccgggcg gctgagatga cggagaggct ggccaaaaca
    41221 ctcggcctcc caagtgtccc gtctcattag aatcttttcc gtgatttccg aggagacggg
    41281 acacttccaa gggggagtca agctccccct tcggcgcttc gctgaacccc tgaggagagc
    41341 cgtgccgccc ccctcaaact cccccggcgg cgccgcgtca cgatgagggg ggatccggcg
    41401 tgttttttcc gagacgcgag gctacgactt gaccatcgtc aggagcatct tgaaccgctc
    41461 gagagcctgg acggcgtggg ggatgtccgc cggcatgagg atggccgccg gggcggagag
    41521 cggatattct tccttatcta tgatgacgag ggcccggcct tcgatgatct gaacgaaggc
    41581 gtcgaaggga gccgagtgag ggctgagctt ttggcccttg tcgaaggcga agagggtgac
    41641 cgtcccggcg tccttgtcga tgatcgtccg ggagacgatc gcgccctctt ggtagtcggc
    41701 caatccggga agatcgagcg gcccggccgt caattgagga tcggagcctt tggatttttt
    41761 catttcgttc tccttaaacg ggggacgaag tcccccaaaa actaccacga aagggtggac
    41821 aagcgcaatg gattcgaaga gccggggccg cttcccccgg gggcggggtt gtatcgcctc
    41881 gagtgaacga tcccgattaa cgcgtttccg cggggaagga ccttcgtctc ggctcgatgg
    41941 ggggttgatc cttcagcgac atctaatgga attcagcggg cggggctgcg ccgcggggat
    42001 taatgtgcga taatgcggga gagaatggcc ggaaacgaaa ggaccgcccc atgagcgcag
    42061 aggagacgaa gctgatcgaa gtccgccggg tttggggact gaccgaggcc gacatcatca
    42121 aggcttttct ggaaagcaac gggatcgatt gcgttttcca aggccgcaac gttctcaccg
    42181 tctatccttt tacgacggac ggactgggcg aggttcggat catggttaaa gatgaggatt
    42241 tggaggcggc ccgggccttg ttggaggaaa ccgatctttt ttccgaatcc gaatagggaa
    42301 gcgtcctccc tttgaggagg cggccccgtt gcctcaatac cggtaatgat cgggcttgaa
    42361 cggcccgtcg accggaatcc ccaagtatcc cgcttgcttt tccgtcagct tcgtcagccg
    42421 cgcgccgatc ctcccgaggt ggaggcgggc gacctcttcg tccagtttct tgggcagcgt
    42481 gtgaacgccc aaagccggct tttccccggc cagccagatc tgggccaggc attggttggt
    42541 gaagctgttg ctcatgacga agctgggatg cccctgggcg cagccgagat tgaccaaccg
    42601 gccctcggcc aggacgatga tcgaccgccc cgatttcaac gtccagcggt ccacctgggg
    42661 cttgatgttc tcccggacgc agccgggcgt gttttccagg taatgcatgt cgatttcgct
    42721 gtcgaaatgg ccgatgttgc agacgatcgc ctcgtccttc atcctctcca tgtgagctcc
    42781 ggtgaggacg tcgcagcagc cggtcgcggt gatgaagatg tcccccccgg agaccacgtc
    42841 gtcgaggggg acgaccgggt agccttccat ggccgcctgc aaggcgcaga tcgggtcgat
    42901 ttcggtgacg atgacctggg ccccgaagcc gcgcatggat tggacgcagc ctttgccgac
    42961 gtctccatag ccgcagacga cgactttctt gcccgcgacc atgatcccgg tggcccgctt
    43021 gatcccgtcg gccagagatt cgcggcagcc gtagaggttg tcgaacttgg acttggtcac
    43081 ggagtcgttg acgttgatcg cggggaagag cagggacccc tcggcttgca tttgatagag
    43141 gcggtggacg ccggtcgtcg tttcctccga gacgccccgg atccgggcgg ccgcggccgt
    43201 ccaacgcccg ggggagcggc ggacggactc tttcagccgg tcgatgacga tccggagttc
    43261 ccggttgtcg tacgtccggt caagcaggga ggaatccttt tcgaccttga ccccccagtg
    43321 gacgaacagc gtcgcgtcgc ctccgtcgta gacgatgagg tccggaccgg agccgtcggg
    43381 ccaggtcagg gattgttccg tgcaccacca gtactcctcc agggattcgc ctttccaagc
    43441 gaagacggcc gccgatccgg cctcggccac ggccgccgcg gcgtgatcct gggtcgaaaa
    43501 aatgttgcat gacgcccagc gaaggtccgc ccccagctcc cggagggtct cgatcagcat
    43561 ggcggtttgg acggtcatgt gaagactgcc catgaccttc atgttccgga gcggcttttg
    43621 gcttccgtat ttctcccgga cggccatcag tccgggcatt tccgcctcgg ccaggcggat
    43681 ttccttccgg ccccagccgg ccagggacag gtcggccact ttatagggaa gcgaccggtc
    43741 caggggaaga acggtttttt tctcggtgtt catggcattc tccttgagcg gtgaagccgg
    43801 ggtcatttgc gaccccggaa aagaaggatt ttcaagcccg tgggaaggga gagggttgtt
    43861 ttcgtccgga cggacagacc ggatccggtc agccaatcct cgagagtctg aggttcgaag
    43921 ccgagccagc ggtcttgaaa gcgggtgcgg agcgtttcgt cctggtgggc gctccactcg
    43981 acgacgacga gcgtcccccc cggcttcaag atccgggcgg cttcggcgag gccccggccg
    44041 ggatctggaa gatgatggag ggccaggcac atcagggcgc aatcgacttc gccgtcgcgg
    44101 aggggcaggc tctccagctc gcccatccga agctccagga tcccccggcg gctgtcggac
    44161 aggtggcgcc ggcgggcttc ctccagcatg cgcggcgacc ggtcgacccc gatcacccgg
    44221 ccggctttcc ggcgcaggac ggcgaagaga tcgccgctgc cgcatcccag atcggcggcg
    44281 acctcgccgc gcgggacatg ccggaggagc aggggatcca aggccgcgtc cccgatcagg
    44341 gcgcgcttca tctcccccca atccccggcg atcgcgtcga aaaaacgccg cgattcccgg
    44401 ttccgatcct ctaaaatttt acgggcccgg gcgaggtccc gggcgaaaat accccgatcc
    44461 ttttcgccga tcgccgcggc gaaccgggcg gccgggccgt cggccgcggt ccggtaaaac
    44521 gtccacattc cgtcccggcg ggacgtcagg aggccggcct cggccaggat cttcaggtgg
    44581 cgggagacgc gggattggcc catccccagg gtttcgacga tttcgttgac gttcagctcg
    44641 aacgaccggc cgagctgaaa caagcgcaga cgggtttcgt cggccaaagc cttgaggctc
    44701 cgcagcgaat cggacgtctt cccgtctttc atgtttttca atatatcaag ataaattgat
    44761 attgtcaagg gtggggatcc gcgattttgc ttgactcgag gggggggatg agtagaatga
    44821 gcggagtaaa gccggatcat gttttttctt ggattattcg cgggctgggc tttccaggcg
    44881 gccctggcgg ccgccccggc gccggtctcc ggaggcctgg ctcccgcgcc cttcttctcc
    44941 gccctggcct cgcccgcctc gcttctcgat ctggacgacg acgctctgcg gcggcgcgtt
    45001 gaggaaaatc ccggttccct gggctcgctt tcgctcggca cgccggacag cgccgtggtt
    45061 atcaacagcg tttccctgcc ggccgatccg cgctgggaat tccttccgcg ggccgagtgt
    45121 tgggcgacgg cggaaaccgt ggaggcggtg cgggcggtcg tggccaaggt ccacgaagcc
    45181 tttcccggca cgcccccgat cgtcatcggc gacatcggcg atcccgacgg cggccggctc
    45241 aagcgccatg cctcgcacca agccggccgg gacgtcgatt tcggattttt ttacaaaacc
    45301 gggacggctt ccggttttcc tgttggaacc gcggcgacgc tcgatctgcc gcggaattgg
    45361 gccttggtcc gggccatcct cacctgcacg gacgtggaag tgatttttct cgacacgggg
    45421 atccaaaagc tcctctacgg atatgccgcc gccatcggcg aggataaggc ctggctcgac
    45481 cgcgttttcc gctatcgaaa gggatcgtcc tcggccgtca tccgctacgc ccgcggacac
    45541 cggacccatt atcatgtccg tttttacaat ccggtcgccc aggagctggg ccggcgcgtc
    45601 tacccgatgc tggtccaggc gaaaaagatc aagccgccgg ttttcacggt ccgccatgtc
    45661 gtccgggacg gagagacgct cggccatctc gcccggcggt atggaacgtc cgtccgggcg
    45721 atccagcagg ccaacggctt gtcgtcgtcg atgatccggg ccggccggtc ttaccgcatc
    45781 cctctcaagg gcgtttccgc cccgccggtc aagcccctga tcgtcccctc gcgccttctt
    45841 cccccctcag ttccggatgt tctagccgcc gcgtcttggc cgtccgccgc ggcgttcgac
    45901 cggcaggcgc tcctcaagct gatggaggcg gcgatccagg gcgcggccgt cgtccgccgg
    45961 gtctaacggt tccgttgctt cggaatcgga aaatcgtgtt tcgccaggta atcgcagaaa
    46021 aagcagcggt cttccgtttt gcccggaaac gatgatccct gagcttcaag aagctcccgg
    46081 atggtcgcca agcccgtgtc gaagcaagct cggacgacgg ggctggcttg ggcccggcgg
    46141 agaagctgcc gatacccgtc ggccgggatg cggccgatga tcaagccgcc gttttcattg
    46201 gcgaatccgc agcagacggc gaccgccccg tccggatgga cgtaaaaaac gtttccgggt
    46261 ccccggcaga agtcgtcttc gaaccagtgt tccgcggtcc aggcgttttc ctcggccggg
    46321 gccgaatagg ggacccggat gatgtcgatc gccagggcct caaacgggcc gtctgccgtc
    46381 tcccggggat tcatgaacgc gtcgttgacg atcgaatagg ggattccctc gtcgatgatc
    46441 agccgtccgt cgaaccgttc ggccagtctt tcgaaggtct cccgaggctc ggccccgtcg
    46501 ggggggatga ccgtgacgat ttcacagcca tcccgccggc cccagacgga gaagacggtc
    46561 tcgaaaaaga gcgtcagatc ccggatgtcc tggtcatggt aagcgtcgat gctgactccg
    46621 aatttcccgt cgaacccggc ttcccgcagc ccggtcagag tccggcggag atgatcgggc
    46681 gagtcgaacc agacgccgtt ggtcatcaac cgcccgaaga gcatgtccat ctcgacggcc
    46741 gcccgcgaga cttcgttgag gaaatccgga cggaggaaag gctcgccgcc gctgaaaccg
    46801 acgcgctcca ggccggcggc ccggcacgat ttcatgaagg cgacggcgtc gcgcgcttcc
    46861 aggcggtcgg ggatccgggt gacccggcaa tgggggcaag cgagattaca gtcggccgtg
    46921 ggcgcgaata tgatttcttc cggtttgaac atcaggcttt ggaggcgtag agacccgtgc
    46981 gcgtgtccac gcggatcaaa tcgccggttt caagatactc gggcacttgg acggccatgc
    47041 ccgtcgacag gacggccggc ttcgtccggg ccgtcaccga gccgcccttg accgaggggc
    47101 gggtctcgac gattggaagg gtgacgagcg tcgggagctc gagccccagg atccggcctt
    47161 cggaggacaa ggccaagacg ccctccagcc cctctgtgag gtaaggccac tcctcctcga
    47221 tctcttcctt ggccagggtg aattggctga aatcctccaa atccatgaaa gagaagctca
    47281 aggcgtcgcc gtaaaggact tggacgggcc ggcgctccag ctcggcctcg tcgaaaaggt
    47341 cgtctccccg gacggtcacg tcggttttcc tcttggtccg ggcgttgcgg aagcggaact
    47401 tatagagggt cgcggccccg cgggcgctcg gcttctggac gttgacggtt tcgacgaggc
    47461 aggggtcgcc ctcgatttta acgacgttgt tttttttgag atcggaggcc ttgaacatgc
    47521 cgaaagattt taccatgttc agccggccgg cgccacagag ttctcgcggc ttgggaaaaa
    47581 aagccccgtc tcgccgtcca gcttcttcga tgggccgaac ggcggaacgg ggcttccgga
    47641 cgccggtcaa cgtgcgcccg gaacaatcct caggaggcgg ccgtggttac tgtttcgggg
    47701 ggacgatcgc gtccttggcc gtcttggcga tccgcatttt cacgacggtc ttggccggga
    47761 tgttgatcgt cgcgccggtg gccggattgc gtccgacgcg ggcgttgcgc ttcaccagga
    47821 cgagcttgcc gagcccggga agcgtgaagc ttccggcttt cttggtttcg gcgatggcca
    47881 aatcgacgag ttcctcgagg aattcgcccg cggctttccg gggcatctcg aacttctcgg
    47941 cgaagtgggc gacgatttgg cttttggtca taggtttctt cggcatggtc cttcctttca
    48001 ccgggtgaat tataagacgg cggaaaatat actctacatc gaaatgaata tcaaccctcg
    48061 gacgaaattt ccccgtccga cggctggcgc ggccgggcgg ccgcgcatat aataggcggc
    48121 atgatgtctt cgacaaggag gatggacgaa ggagcccgga tgcgccggac gtttgccgta
    48181 tcgacggccc ggctgcggat catgatggag gccttccgcg gcgagatgcg ccggggcttg
    48241 aacggcaagc cgtcctccct gcggatgctt ccggcttatg tcgaccgccc cacgggccgc
    48301 gagcggggga cgttcctggc cctggacctc ggcggaacca atgtccgcgt tcttcaggtg
    48361 gatcttccgg gagacggacg tctccccgtc tgcgccgagg atcggtttgt gttgagccgg
    48421 gacaaggtga cggccgacgg ctccgtcctc ttcggggcgc tggcccggtt catcggccgg
    48481 tttctccggg agcggagact ctccggccgg ctccccctgg gcttcacctt ttccttcccc
    48541 ctccgccagg cgtcgatcgc cggcggcgag ttgatcgcct ggaccaaggg gtggacggcc
    48601 gaaggcgtcg tcggccgaga cgtcgccgcc ctgctcaacc gggccttggc cgccgaaagg
    48661 atctccggcg tccgggttgt atccctgaat aacgatacga ccgggacgtt gatggcccgg
    48721 gcctacctcg atccggattg cgacgccgga tgcattcttg gaaccgggac gaacatatgc
    48781 taccgcgaac ggacctcgga aatccgcaag cccgtcggcc cgcacggccg ggatcacatg
    48841 atcatcaaca tggaatccgg gaatttcaac gccgtcctgc cccgcaaccg cttcgacgtc
    48901 ctcttggacc gctcctcggc gaatcccggg cttcaggcgg aggaaaaaat ggtttccgga
    48961 aaatacctcg gcgagctggc ccgtctggcc gtcgtcgagc tgtgcggccg cggccggttg
    49021 ttcggcggac ggcttccgga aatcttttcc cggcgggagg cgtttggaag cgaacgcctc
    49081 tcggagatcg agaaggacga ttcggcttcg gccgacggcg tccgggagtt cctttgggat
    49141 tggggagccc gtccggcggc cgccggggac gcccggcttc tgcgggatgt cgtccgggcc
    49201 gtctccggcc gggcggcgcg gttggcggcc gcgacgctgg gggcgacggt gacgaaaaac
    49261 gacccggcct tggaggcgaa acactcgatc gccgtcgacg gaagtgtttt tcataaatat
    49321 cccggattca aggccaggat ggaggaaacg ctccgggagc tgttcggggg ccgggggggc
    49381 cgcatcgctc tcgccccgat gcgggacgga tcgggattgg gagcggcggt tatcgccgcg
    49441 gtcgcggccg agtcggccaa aaccggcgga aagaagcccg gcgccggctg gtgagcgctc
    49501 ggcgaaaaga cttgtttcaa gcccaaaaaa atcgcttgct tttatttttt ttctgtttat
    49561 gatattagcg tcgaaaaaag gaaggggcac gggccctgtt cggctgtata ctcctctcct
    49621 ttattcgaac aaatccaagg gacgtccgga gtgccgcccg gacggagccc tcgtcaaagg
    49681 agacgagtat gaatatctat gttggcaacc tttctttcag tgtaagcgaa accgatctgc
    49741 gcgaagcgtt cgccgcttac ggccaggtcg cgaccgcgtc gatcatcaag gacaagttct
    49801 cgggtgaatc ccgcggcttc ggctttgttg agatgccgac caaggaagag gccgagaagg
    49861 ccattgccgc cctgaacggc aaggacctca aaggccggac gatgacggtc aacgaggcga
    49921 agccccggac tgatcgcccc cggacgggag gcggcggacg cggcggcttc ggcggcggcc
    49981 ggcgcttctg attccggttt tgtcgatgtc gttccggccc gggccccggg ccggatgtcc
    50041 ctaagacaac gaagggcggt tcttgggaac cgcccttttt tttatttgcc gccgggcgcg
    50101 tcttccgaga ccggcggaaa ggaggagtaa tggcgaaaag cagccgcgct tataaaagcg
    50161 ccaaacgaag caaggagctc gaccgcctgc gcaagcggga agagaaacgg cggcgccgga
    50221 tggcggtgaa atccggagga gccgccgagg aacaggtcgc ttcagccgaa accgcggcgg
    50281 agcagcccgc cccggaggaa gatcccgatc ccatcatccc ccgggactga cgtcctcaac
    50341 cgtttccgcg tttcccgtcc cttctcgcgt cctttcggcc cgcctgggac ggaaggcctt
    50401 tcggtttcat tcggacgcct ctatcggccg cgcgcgtccc gtcatccggc cgggacgccg
    50461 cccgcggcct tcgcttttcc tttgacatga accccggttc cgcgctatcg tgaagcgcga
    50521 gcaaggagcc gtcatgccca gcaagccgaa gaatccccac cttccgttcg gggatcaaca
    50581 gaccgccccc tggtacggga agaatatcct ctccgtcaag cagttctgcc gcaacgacct
    50641 ggaatatatc ttcagtgtcg cccacgagat gaggacgatg gtcgagcggg tcggcacgtt
    50701 caacctgctg aagggaaaaa tcctggccaa cctgttttac gaaccgtcca ctcgaacctc
    50761 ctcgtcgttc atggccgcca tggagcgcct gggcggggcc gttatcccca tcagcgaagt
    50821 gaaattttcg tcggtggcca agggggagag cctcccggac accgtccgca ccctgggctg
    50881 ctatgccgac gtgatcgtct tgcggcatcc cgaaatcggc gcggccgccc tggccgccaa
    50941 gtatgccggc aagccggtca tcaacgccgg cgacggggcg ggagagcacc cgacccaggc
    51001 cctgctggac acgttcacga tccgcgagga gctggggcgt ctggacaacc tcaacgtgac
    51061 gatgctcggg gatttgaaat acgggcggac ggtccattct ttggcccggc tcctgaccca
    51121 gttcaacggg atcaagctga attacgtttc ccccgacatc ctccgcatgc cgcccgacgt
    51181 catggaagag gtggcggcca aggggcttcc ccaatccgaa cactcaaccc tggacaaggt
    51241 tctgcccgaa accgatgtcc tctacgtcac ccgggtccaa agagagcggt tcgaggatcc
    51301 ccaggcttat gaaaaggtca agaacgtctt tgtcgtaaat cccgcggtga tgaaagcggc
    51361 cggcaaaacc atgatcgtcc ttcaccccct gccgcgcgtc accgagatca gcttggactt
    51421 cgacgacgat ccgcgggcgg cctatttccg ccagatggaa tacggtcttt acgtccgcat
    51481 ggccctgctg gccatggtcc tgggaaaagc ctaagccgtc ttcttctcct ctttcaaccg
    51541 gtttccccgg cggccgcccg gccggccaat gtccccgagg cccaagccca ggagagattg
    51601 aatcctccgc gcggtccctg gacgtcgaga atttccccgg cgaaatagag tccgggcgct
    51661 tttttcgagg acagcctctc ccggtcgatt tcccccgcat ccactccgcc gctcgtgaat
    51721 tcggcctcgt tccagccccg ggttccctga acggcgaacc gcttgccttt aaggaccgcg
    51781 gcgagagcgg ccgccgttcg ggccggacgg ggggggccgc cggggatcaa gccggcgatg
    51841 gtccggccga atttttccgg caggaggccg gaaacgagat ccgccgctcc ccacccgccc
    51901 tccaagcggc gggcgagctc ggcggacagg gcttcggcgc tcatgtccgg gagcaagtcc
    51961 aggacgactt cggctcgggc ctttttcccc cgatgaagag cgattgaaat gtccgtgctg
    52021 acgtcgagaa cggccgttcc ggaaaggccg taggcggtga agaggacgtc cccaagcgcc
    52081 tcggccgccc gctgcccgtc gatccaggct tccgcttggg cccggactct ctggccctgg
    52141 agaaagtggc accaggggtc ccggacgagg agggggatgc agctgggaac gggctcaatc
    52201 aggcgatggc cggcggtccg ggccagggcg taggcgctgc cgtccgaccc gagggcggga
    52261 taggaccggc ctcccccggc caggatgaac gcccgggcct cgatcgtccg gccgtcggac
    52321 gcggccgcgc gcgaggcctc tgcgctccgg atgatggaaa cggcctcgaa tcccgggatg
    52381 acgctcaccc tccggcggcg gacctccatt tccaggacct taagcaccga ggcggcctga
    52441 ttggtcgccg ggtagagacg gtctccgtcg gacacgatcc ggaggccgag cccttcgaaa
    52501 aacgagacga tctcccgggt tccgaaacgg gccaggaccg aggcgacgag gccgggattc
    52561 gtcgaatgaa agaggcccgg atccaggcgc tcgttgccga ggttgcaccg tcctccgccc
    52621 gtgatcaaga tcttttttcc caggcggggc atccggtcga cgatcgcgac cgagcggcct
    52681 tcccgggagg cggcgatcgc cgccgtcaag ccggacgccc ccccgccgac aacggcgata
    52741 tcaaagggcg cgcgcatgcg gcaagcatac tgcattcgac cggcttcgcc aagccgggtc
    52801 gccggtttgt caaggcaact ggatcaaggg gacccgcttc caggaagagg acgtcgaagg
    52861 caggacatga tcatgccgcc gaaacagatg ggccgccggc tcagccggac ggcagctatg
    52921 tccgattgtt gtccgagccg gggcgacggc cggaatgatt ttccgaggga tgatccggcc
    52981 gtggccgaaa acgtcccgaa gatcatcggc ggggtccgct gcgtccttcc gggcgcgcga
    53041 gggcccgtct tgaaggccgg attgcctgga attatgattt gcgcggcgtg gctccgtcgg
    53101 cgcagggacc gtcctcgtcg cccgttcggg ccgattcggc ggaaacgggg accgtgcaga
    53161 cgggaagggg ctcgggcgtc atggccgatc cgcagcaggt tggaacggac gccgaggtcg
    53221 gggccgaggc cttggccccg caggcggggc aaacgtacgt cgtttccttc atggtttttc
    53281 tctccaggaa atattatagc aggcttgctt cccgccgtcc cgtgctccgc ctcgggacgt
    53341 ccgcggcttc ggcggagaag aacgccgccc ggttcaggct ttccgtttga ggctcgaggc
    53401 gagcagctcc gagagggcgg ttttcatcga cgaaggaagc gaggcctccc ggatccgccg
    53461 ggcgcagcgt ccggcgatcc ggcggacatc cgcctcgatc cgttccggga tcttttcggt
    53521 ccggagaagg gcgttgaggc gggagatatc cccggccgcg gggcggcgtt ttccgaacag
    53581 ggcggcggcc cggggtccac gggcccggct caagtaaaaa taataaaggg tctttttccc
    53641 ttcccccacg tcggagccga cgggttttcc cgtggttgag ggactgccga acatgccgat
    53701 ctcgtcgtcc ttgatctgga aaacgagccc caaatcgagg ccgatgtctt cgagaccccg
    53761 gatcgcgctc tgtccggcgc cggccaagat ggctccggcc gcgagcggca gcgaataggt
    53821 gtaccgggcg gtcttgaagg catagaggcg gtagatgccg gctcgggata cgggccggcg
    53881 gatccggccg gaatagacgt cctggacctg ggccggaccg acatgggcca gctcgcggcc
    53941 gaacagcgag agaacccggg ctttatccgg ggcgggagcg gccagagagg cgaggatctc
    54001 caggcccatg aaaatcagca cgtcgccggc gcacagggcg gccgcccggc cgaagcccgg
    54061 ggcgtcgttt acgcccttcc gccgggcctg ggccgcgtat tggacatgga tgctcggctt
    54121 tccgcggcgg agcggatcat cgtccatgat gtcgtcatgg atgaggaagg ccgagtggaa
    54181 aagctccatg gccgcggccg ccttaaccgc gtccgggccg gcttttccgt cgagccggtc
    54241 atgggcgatc aggatcaggc cgccgcggat cattttcccg ccgaggacga acggccggat
    54301 gcgccgggtg aaatccgcgc cccaggcgtt gaccgcggcc aagccggccg cccggccgtc
    54361 gaaatagcgc gagaggcatc gcgcgatccg cttccggctc ttttcgaaat cgcgatccaa
    54421 tgttgatccg gtcatcggct ttttgtctcc ctctccaagc ggcgccggag ccgggcgacg
    54481 gtccggtcga tgacgtccat cggcgtgcta gcccccgaca gaacatagaa cttccggcct
    54541 tgtccgagcg gaagaccgtc gacgtcctcc ggccggctga ccaggcgggt ccgccggttc
    54601 cccgcgccgg cgatcgacat caggttgcgg gtgttggcgc tggccgggga gccgatgacg
    54661 atgacgccgt catagcgggc ggcttggatg cgggcgtccc gctggcggtc tgaggtcggt
    54721 ttgcagatcg tgttttcgaa ccggacgtcg gggacgaaac ggcggatttt cctcaggatg
    54781 gcgtcggcct cggcccggat gaacgtcgac tggatgacca ctccggcccg gtcgacgccc
    54841 tggaaaaatc cccggccggc ttcctccggc gtgcccagga tgagagcgtt ttcgacctgc
    54901 ccggcgattc cctggacttc ttcgtgccgg cggtcgccga tgatgatgac gcggcggcct
    54961 tcgccggcca gccgccgggc ccgggcttgg atatccttga ccatggggca agtcgtgtcg
    55021 acgaaggaga tgcccgaggc cttgatctcg ctcaaacgag aggctggtat cccatggctc
    55081 tggatgatca gcaggccgtc ctcggccaag cggtggtcgt cgatggtctt gaatccgcgg
    55141 cgtttcatcc ggttgaggac gatttcgtta tggacgacct tgcccagaag ataaatcttt
    55201 ttccccggct gctcgagccg gagcttttca atgattttga gagtccggtt gaccccgccg
    55261 cagaagccga catagggcga aacgtcgacc ttgggagacg cggatttatt catcggccgt
    55321 ttcctggagc tttttgaatg attcgtcatc ggggctttcg ccgaccgccg ggacataagc
    55381 ggccgaggcc gccatgatca gtacggcggc tcatcttccc ttcggggatc atatccaata
    55441 aaaatttata ccaaaggaat ccgacggata caagaaggcc agcgcctcgt tggatcgcgg
    55501 attgatcccg cctttcccag acaaacggga aacgctcttt atcgagattc gcgaggccga
    55561 tgacgttcat gccggattta tggaatcccc gtccccgggc gatcaacttt tcattcgggc
    55621 gacggatttc tttttcggac tcaaaacgat gatccgtatt gggagaaaaa aggacgcgtc
    55681 cgcccgaggg gatcgtcgcc aaaggacgat cctcgcgatt ctcttcagcc ggcggattga
    55741 cgactcggca tttcgcccgc cacggccgct ttttgcgctt caccgggggc tttcccggct
    55801 tcgacacggc ggtggcggcg atttgactta tccgccctga aaagctagaa tcaatgactc
    55861 cctgaaacgg aaaatttcgc cttgacgaga accgaacatc agggggaaag accgtcatca
    55921 tctcactttc atcatcatag gaggagaccc gatggagaaa cccttcaacg ctttcgagat
    55981 ggctcagaaa caattcgacg cggcggccga taagctcggc ctggaccagg cgacccgcga
    56041 actgctccgc aaccccctgc gcgaatacca tgtcctgatc cctgtccgca tggacgatgg
    56101 gagcaccaag gttttccggg gataccgggt tcagcacagc gacgcccgcg gtccctgcaa
    56161 gggcggcgtc cgttttcacc cccatgagac cgtcgacacc gttcgggccc tggcgatgtg
    56221 gatgagctgg aaaaccgccg tcgtcgacat ccccctgggc gggggcaaag gcggcgtcat
    56281 ctgcgacccg cacaacctca gcgaccggga gcaggaaggc atctgccgcg gctgggtccg
    56341 ccagcttgct ttcaacatgg gccacgagca ggatattccg gcgcccgatg tcatgaccca
    56401 cggccagcac atgctctgga tgatggacga gttcgagcgc atccgcgggg ccaagttccc
    56461 cggtttcatc accggcaagc ccgtcggcat gggaggttcg ctcggacgga aggaagccac
    56521 cggcttcggc gtgatcttta ccgtccggga agcgatgaag gagctcggca tcgacattaa
    56581 gaaagcggtc ggcgctttcc agggcttcgg caacgtcgcc cagcacgccg tcaagctttt
    56641 caaggaatac ggcggcaagc cgatctgcgt cgcctgctgg gaccaagccg accagacgtc
    56701 ctacaccttc cggcgggaaa gcggcatcga ccccgacgag ctcgtggcca tcaccgacaa
    56761 gttcggaacc atcgacaagg ccaaggcccg tcaggccggc tatgagatcc tcccgggcga
    56821 tgcctggatc gagcaggaag cggaccttat cgtgccggcg gctttggaaa accaaatcac
    56881 cgcggccaat gtcggcaaga tccacaagcg cgtccggatc atcgccgagg cggccaacgg
    56941 cccgacgacg cccgacgccg acgccgagat catcaagcgc ggcattttcg tgatccccga
    57001 cttcctctgc aacgccggcg gcgtgacctg cagctatttc gagcaggttc agtgcaacat
    57061 gaactatttc tggccccagg atgaagtcct ctccaagctg gacgaaaaaa tgacggtcgc
    57121 cttcaaggcc gtctccgagc tggcccggaa aaagaacatt tatatgcgcg atgccgctta
    57181 cttgatcgcg gtgacgcgtg tcgccgaggc cagccacaag cgcggctggg tatgattccg
    57241 gcgtcccgtc gtttttccta gatcgctgcg cggccccgcc cccgaaggcg gggccgcttt
    57301 ttttttcggg gaggtgaggc atgagccttc gcgagtcgga cgtccccaaa ggcccgctgc
    57361 gggagcgggt caaggagttg gagtgcctcc aggccatcgt caccgtcatc gaaacgcccg
    57421 gcctgtcctc ggacaagatg ctccagcgga tcgtcgacgt catcccctcc ggctggcagc
    57481 atccggagcg gtgccgggcc cggatcaaga tccataaggc ggtttttcac acccctggat
    57541 tcgtcgagac gccctggatg cagcgcgtcc ccctccgggc ccgcggggag acgatcgggg
    57601 cgctggaagt ctgctacacc gacccgggcg gctcccccca ggaaaacgcc ttcctcctcg
    57661 aggagaagaa gctcatcgac ctgatcggca accggctcgg ccgcttgatc gaggagggcg
    57721 aaggcgccgg ccgggcggcc gcggccggac ccgccgagga attccccgga aaacgaaaac
    57781 ccgaatggcg ggtcatcatg gatctcttga gagagaccga tccgatgctc taccggcggg
    57841 ttctccggcg gctgatgaac catctccact ggcagggcat ccccggcgtc caagggttgc
    57901 tgttccacct gaccccggaa tcttactccc gggccgaggc cggacccgat gaaaaccagc
    57961 cccagcccgt ccaaagcgtc gaacgcctga gcaaggtctt cgaggaagcc ctttggatcg
    58021 cctccctggc cctgacggac gccgagcttt cggacctcct caagcagtgg atgcggcagg
    58081 acaagctcgg ctttttcatg gtggccacgg aaaagcggga gatttccgtc gttgagatca
    58141 aggagatcct caaccggttc tgccgctcga cgggcgagga cgaacagtcc ctctctccgg
    58201 ctgacgacct ccaagcccgg atcgccctca tccgccgctt cctcagcgag cgtcttccct
    58261 tcatccggat cgcccgcgat ttcatgacca tccacgattt cgggcggctg ctgccccgcg
    58321 tcttcggccc gacctcgggc accgggaagc tgggggggaa atccgcgggc atgatcctgg
    58381 ccgagcatat cctccgccag aaagcccagt ccgacccggc cgtcgggacg atcaagatcc
    58441 cggagtcctg gttcatcacg tccgacgggg tgatggattt catccattat aactacttgg
    58501 aggatttcca gagcttcaag ttccgtccga tcgaggagat ccgccacaac tacccctacc
    58561 tggtccaggt ctgcaagcag tctttctttt cgcccgacat gctcgcccag ctcaagctca
    58621 ttctcgacga cgccggcggg gatccgctga tcgtccggtc ctccagcttg ctcgaggaca
    58681 gccggggatc ggcgttctcc ggcaagtacc ggagcctttt tctggccaac acggggacga
    58741 aggaggagcg gctggacgcc ctgaccgacg ccgtggccga ggtctatgcc tccattttca
    58801 accccgacgc gatcgaatac cgggcggagc ggggcctgct ggattactac gaggaaatgg
    58861 gcgtcctcat ccagcaggtc gtggggacgc gggtcggaaa gtatttcttc ccggcgttct
    58921 ccggcgtggc cttcaacaac aacgagttcc gctggtcgcc ccggatcaag cgggaggacg
    58981 gcgtgctgcg gttggtgacc ggtctgggga cccgggccgt cgaccgggtc ggcaatgatt
    59041 accccctgct ggtcagcccc ggccagccgg gcatccgggt caacgtcatg cccgaccagg
    59101 tgattcatta ttcccagaag gcgatcgacg tcatcaacct ggaaacccgc cgcttcgaga
    59161 cgcatgcgat cgaggagctc ctcaaggaaa tcggcgcgga tttccccatc tgggaccgcc
    59221 tcttttcggt ctgggacggc cagtccgtcc gccgggccaa ccgggtcttg gaccagcctg
    59281 accaggattc cgtcgtcgcc acgttcgatg gtcttttgac cgggacgccg ttccttctcc
    59341 agatgcggga aatcatgcgc gtcctgaagg agatcttcgg gatcccgatg gacgtcgagt
    59401 tcgcccatga cggccgggat ctctacatcc tccagtgccg gccgcagagc cgtctggacg
    59461 acgaggaagc gatcgccatc ccggccatga tccccgacga aggcaagctg ttttcggcca
    59521 ggaaatacat caccaacgcc gatgtccggg gcattcggac gatcgtctac gtcgatcccg
    59581 agggatacgg ccggatcgcc tccctggccg acatgggagc cgtcggcgac gcggtttccc
    59641 ggctcaattc cttcctgccc cggaaatcct tcatcctgat ggggccgggc cggtggggca
    59701 gccgggggga tatccggctg ggcgtgcggg tcgcctattc ggacatcaat aattcggcca
    59761 tgctggttga aatcgcccgg aaggtcggcg aatacgtgcc cgaattgtcc ttcggaacgc
    59821 acttcttcca agacttggtc gaggcccgga tccgctattt ggctctctat cccgacgacc
    59881 gggatgtgat tttcgcctgg gactttttcc gcaaatcacc gaatgtcctg acccgctacc
    59941 tgccggaata cgcgtacctg gccccggtgc ttcgggtgat cgacgtgcct gagacggccg
    60001 acggccggga gctgcgggtg atcatggacg gcgatcggga gatggccctc ggcttcctct
    60061 gcgatccgac gaagggccgg ggcgaggaaa caggcgatgc gggattgctt taatccccgg
    60121 accgggccga cgggcggtcc ggaggcaagg accggccgat gacggcccgg ctgctgctgg
    60181 tcaactgtta ccgggaaaac gcggcgaaga agatcgatcc ctaccgggaa tggattcaag
    60241 ccggggcgag agacttgggc ctgaagcttg aaattctcgc gaagttcgac ctcgggccgc
    60301 ttcccggcgg ggattacgac ggcctgatcg tctccggatc gcgccaaatg atcggccggg
    60361 gacaggtcga ggccggcctg ctcgatttcc ttcgaaccaa ccggcggccg cttctcggcg
    60421 tctgttacgg ccaccaggcc ctggcccgcg ccttcggggg gttggccggg aaggatgcgg
    60481 cgacacatcg gggcgacgag atgatccatc tcgaggcgcc cgatgcgctt tttgccggat
    60541 ttcccgcgtc atttccgatg agcgaaagcc acgaggagat cgtcgcccgc gacgaagccc
    60601 tcgagaaaga gttcctcgtc ctggccgccg gcgactccgg ccgggtcgag gccgtccggc
//
LOCUS       DAOOXH010000005        60237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_411_length_60237_cov_9.388521, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000005 DAOOXH010000000
VERSION     DAOOXH010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 60237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60237
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_411_length_60237_cov_9.388521"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PKV64_01280"
     CDS             <1..496
                     /locus_tag="PKV64_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPN15740.1"
                     /translation="EELFYRLNVISVDMPPLRRRPDDIPLLIQHFLRKHCERMGRKIK
                     RLTPEVTTALTKYSWPGNVRELENIIERLCAVEERETITAESLPDDVVEAGRVPVGGA
                     PVLLPGFDLEAHLDQVAQAYLKEASEKAAGNMRRMAELLGVSYRSLRYLMKKYQIRRP
                     GAAE"
     gene            749..>1263
                     /locus_tag="PKV64_01285"
     CDS             749..>1263
                     /locus_tag="PKV64_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPN15741.1"
                     /translation="MFKNRKGFTLIELLIVVAIIGILAALLIPNAMTALQKAKVRGTQ
                     KDINSIATGIADYITDKAVPFTANGAMDSTVKTSLSPLYLKVLPETDQWGQAFMIYTG
                     TNVSGQYGISSAAVDDFLVASYGRDKTIEGWTYNDTTPDAGLYSIGATADFDKDLINY
                     NGAFIRGPRAGA"
     assembly_gap    1264..1363
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1364..1804
                     /locus_tag="PKV64_01290"
     CDS             <1364..1804
                     /locus_tag="PKV64_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15742.1"
                     /translation="PNAMTALQKAKVRGTQKDISAIATAIAGFISDKTFPPPANGSID
                     IAVNTALSPFYLKVLPNMDQWGQPFMIFTGDNCTQYGISAPGADDFLVISYGRDKTLE
                     SWTYNPATPDSGLYSIGAAADFDKDLINFNGAFIRGPRAGAVGT"
     gene            1859..2560
                     /locus_tag="PKV64_01295"
     CDS             1859..2560
                     /locus_tag="PKV64_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPN15743.1"
                     /translation="MIIRTAVLLLFYAGIILCLIPGLLFAAGLGLRETLVRIGKWAMG
                     ASRAVLGLRVDVFGRVPESEDGPFVYMSNHVSLLDGPLLFRLIRGPVRVIMKTSLFRI
                     PVVGLAMKIIGFIPVDRSGTQGGRAAVSRAAAAIKEKGCSFLIFPEGTRSLNGRLQTL
                     RRGGFFLAREAGVPIAPVSIEGTFEIMPKGRLVPGKGSIRVFFHPPVADPEPGSSDFE
                     AWMSAVRERLSSFPA"
     gene            2575..3144
                     /locus_tag="PKV64_01300"
     CDS             2575..3144
                     /locus_tag="PKV64_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPN15744.1"
                     /translation="MDHQRIAQDWSRMLDEFFVARGETFAGAIEACVSALKKGRKILA
                     FGNGGSAAEAQHFVAELVNKFLKPRPALRAVTLSTDTSVLTCIGNDVSYEAVFSRQIE
                     ALGDPGDIVLALTTSGNSPNVLTALKTAKEQGLLTIVLTGRGGGKSAPLADLLLDVPS
                     TETPRIQEIHLFLLHVLAGEIEERMYPSS"
     gene            complement(3173..5503)
                     /locus_tag="PKV64_01305"
     CDS             complement(3173..5503)
                     /locus_tag="PKV64_01305"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPN15745.1"
                     /translation="MISDKANRIGSSPTFKVSAKAKAMKAEGLDVVDLSIGEPDFPTS
                     ESVKAAGIKAIQDNYTKYTENEGSPALKQAIIGRLKEDYGLTYAPNEVIANCGAKSSL
                     FLAFQALINDDEEVIIPAPYWVSYPHLVNLAKGKPVFVPTKEENGFILTAEELKARIT
                     PATKALLLNSPSNPTGAAYTLAQLEALAEVIRGEDLCVVSDEIYSCLVYDRFKYRSIA
                     SLGDDLKKKTVVINGVSKAYAMTGWRIGYAAGPAPLIAAMSRIQSHATSHPASISQRA
                     SVEALAGSQADVARMRAEFERRRNYSLMKLRTIPGVSCFEPQGAFYLFPNVRSFYDLE
                     FNGTPIRNSYGMAYYLLREAQVAIVPGDAFGADDHIRISYATSLENLEKGLNRIAEAL
                     ARLKPARKAKRVALNNVVTRVRGPVPVDPRLPLNQRDGLTAETEARLAADVLFEWNAA
                     VAGVILRLKTNLRHVYEMWVENWTPAPAEAGIEPHGVLYAVDGLAGRDPRAFFNTETK
                     TGVVVNCDSYAPVRSLALGLAGDVAGRHFGSCLIRGMAVDREGGGVILVGPAGTKKTD
                     LFFSLLRDPRFRFVSADMISARPAGKAVQAEAIERKAWLPTNTVELFGRLARLFDRSK
                     CENVVIRKEDCPNAECLRAEDCRLDRGGPFCYKAAKDARALLDPSWLGPGAAVRRTQL
                     RWLFVLRNDSVSPALEELNPEDALRILERGETPGIKKSAAAVPKPFFNASFLASTEER
                     IEIEKDLFRRLLPNLRTFLFNSGAAGVDKIKETIGG"
     gene            complement(5534..6937)
                     /locus_tag="PKV64_01310"
     CDS             complement(5534..6937)
                     /locus_tag="PKV64_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPN15746.1"
                     /translation="MSLVAILILAGAVLALIVLFVLLFARQYRKVGPNEALIISGRKR
                     TVALPDGTAKKVGFRVRIGGGTFVRPFTEKVDILPMDVIPISIRTPEVLTHGGVPIVA
                     DAAAQVKIDSSEASIHTAAEQFLGMGKEGVRDVAVNVLEGKMREVIGTMTVEEIYRGR
                     SEFNRRVGEAVRYDFGKMGLVLLSFALKEFGDTQGYIDALSKPLISAAKRDAAVAEAE
                     TEKEAIIRSSQARKEGEVARLQAEAQIAKAQWENEAKKSESLTWVNQKKAQADFSYEL
                     ERHRLSQELKREESKVRLVEKEQAIKIEELEIQRREKELDSNVLKPADARKYQIRAEA
                     EAEEYRIQAEARGKAEAVKLESQAEAARIKEKGAAEAEALTKRAQAMNHYQEASVLEM
                     YMNVLPDVVKSVAEPLSKIDKIVLVNSDKDLGVSKISAQTAQILAQVPEILQSLTGAD
                     LKKFLKDKLTAEKKSEK"
     gene            complement(7051..7821)
                     /gene="rph"
                     /locus_tag="PKV64_01315"
     CDS             complement(7051..7821)
                     /gene="rph"
                     /locus_tag="PKV64_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HPN15747.1"
                     /translation="MKNPNLRPGRSGSRAADQIRPVKITPDFIEVADGSALIEMGRTR
                     VVAAATIEEKVPPFLKGTGQGWVTAEYAMLPRATEKRTPRERQMGYPSGRSQEIQRLI
                     GRTLRAVTDLRALGDRQIIIDCDVLQADGGTRVASINAASVALALALKKLLDTKILPV
                     LPLRHLVGAISVGIVGGDVLCDLDYSEDAAAETDMNVVETDRGQLLEVQATAERVPFS
                     RHQLDSLLKMADAGIVEVIQAQKDVLKKKSLMFMAYGR"
     gene            complement(7808..8728)
                     /locus_tag="PKV64_01320"
     CDS             complement(7808..8728)
                     /locus_tag="PKV64_01320"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPN15748.1"
                     /translation="MPKRRPDSSSVGIHLSYVLYRLFASFVARLPRRIVLLFSEALGA
                     LVYHLDSRRRRIALDNLSRAFGRSLSHEDRRRTARRSMMNFVRSLFDILKTAPWPPGR
                     VEALIDVQGKENLETALARGRGVLLFTAHFGNWEMVITPIARRVPFHVIVRALDNPLI
                     DRELESVRARRGATVLNKFGAGRPVLRALGRKGAVGIVIDQNVLRREAVFVDFFGIPA
                     ATTPALAVFHLRTGAPIVPMFCVPAPGDRYRLIFGPPVDLPLTGDEAVDVLKITAVCT
                     KMIEDAVRRDPDSWLWIHKRWQSRPHDEKS"
     gene            complement(8721..9728)
                     /gene="lpxK"
                     /locus_tag="PKV64_01325"
     CDS             complement(8721..9728)
                     /gene="lpxK"
                     /locus_tag="PKV64_01325"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPN15749.1"
                     /translation="MRILLRVYSLVSRPVCAGKAARYRRGRKTPARAPLPILSIGNLT
                     LGGEGKTPLACEIIAHFLRKGWRPALVSRGYRGAWERSGGVVSDGKTFFGGFREAGDE
                     PVLVARRYPRAGVFVGRGRLASCRAAASLGFDFCVLDDAFQRLDLARDLDIVLHDPRS
                     SSPRREGDRALARADVLLLPRGTSPERFEAYRRRFPRLKVFEYAAVPRGLFSADGQTG
                     VPLEALSGKRVLAFAGIARPRRFFSQLESLGAAVVGRMTFPDHHLYPAASRARIAAAV
                     SRTDPEMVLTTEKDAVKFEAGELPWEGPGLTVLRIGLDLPDEFFECLEAAVFPGKAGH
                     A"
     gene            9878..10744
                     /locus_tag="PKV64_01330"
     CDS             9878..10744
                     /locus_tag="PKV64_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15750.1"
                     /translation="MKRRFNFWIFALAAVLVLTGCQKKTPIKGVSLDVVFAESPLTDN
                     LITEVTYTWKTGKDFEKFSRDYHVFVHFWHNDNLILQDDHAPAPPTSSWEPDKTYTIT
                     RRIFIPKFIDEFNPQFKGEETLRFSVGLVNPFDRSGDPPREVLIQKLKVVPPPIGTPE
                     IIYESGWYDLEIDQNAPLKQWRWTAKEAKCIIDNPRRDAVLVIKGAANIADVKDQKII
                     FKINDLTVDEFIPGESLFEKTYRIQKEMLGDKDEFILSIGANPSFIPADIRPASKDAR
                     ELGLQVSFIYFR"
     gene            complement(10760..11311)
                     /locus_tag="PKV64_01335"
     CDS             complement(10760..11311)
                     /locus_tag="PKV64_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15751.1"
                     /translation="MKFRKAVIAVVLLGMAASASAQDLAAAARKEKERRASLKGKPSV
                     TVTNTDLTKATKKAAISETRTEDGEQTAAAEGAPAEPEMKPSAVAAEPSQALVVETAV
                     DVEGLRADLQDKYDRAKERLEYVTLKMRALQQQFFSFNTMESKAKVQQDIAQTNLDIQ
                     AAAAEEARLKKELEDFDSKNPGK"
     gene            complement(11381..13753)
                     /locus_tag="PKV64_01340"
     CDS             complement(11381..13753)
                     /locus_tag="PKV64_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HPN15752.1"
                     /translation="MAYPVLFLAIAVSAGIAAAGFLSLSPAAAAAATAVVLAAAWILF
                     ARRKTAAAFVLLLTAAAGLGAGLGSAFEARYESNELRSLPTGEYFDFTGTLSLSPAPG
                     LDRDDLYLRVESVSLRGADRPMTGRIRVAVKHSATGGKLPRLIAGDRVRLSAQVVPPS
                     EYWNFKAPFSVAYMKTRDLHALGSTKSPSLVEHLGPGRRSVPFRWISRLRRTCLDLIE
                     RSFADPGRPGGISPEGAVFEALVLGERSRMDEATTRDLQKSGLFHLFAISGAHIAIVS
                     ALLFFLFRAFRIAERPSYLLLLLLLVFYGFLVEGRASVIRAVVMAAAFIIGKLFWKDV
                     HLLNTISLSALGILFVRPFQLFDAGFQLTFAATLGLILFFPSIRAALPRLPFKIEELF
                     ALSVAAQMAVLPIIAATFHRVIFSGLILNLIGIPLVTVVMATGYVFLPLSFVFPFAAG
                     IAASGLSFLLRILLDSTRLLDGLPFLSSRVPTPPGPVVAAYYVFLLLLLLPARFVRLR
                     RFSFAGFAAALIVLTTHPFPADVKDLTVTVIDVGQGESVLVEFPGSEKMLVDGGGLPT
                     GTFDIGEHVVSPFLWNKGIKKIDRLVLTHPHPDHLNGLAAVARNFSIGEFWEGTPAPE
                     DARYRALREELGGVPVRGVGRGFNHNLGGVDIAVLAPAETSGAKAPGNDSSIVLRLIY
                     GSTAFLLTGDIGRDVEREILAAGLDVRAQVLKAPHHGSDSSSTEEFLEAVRPEVIVVS
                     AGRGNRMGLPHPAVLDRFGKCGARVLGTDRQGAVEIRSDGRELSIRTSRR"
     gene            complement(13761..15065)
                     /locus_tag="PKV64_01345"
     CDS             complement(13761..15065)
                     /locus_tag="PKV64_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPN15753.1"
                     /translation="MRKLFPAITLLFAFLCPSLSFSAVDKKTAADIDRETEKIAAEMV
                     KIRRLIHMDPQLPGREQETAKIVAARLAALGFEVKTGVARGGIVGLLRGAQPGPIVAL
                     RADMDAVPIQELADVPFRSVNGGVMHAHGHDIHVAVALGSAYVLNALRDRMKGGVKFI
                     FQPGPDPASEDPESGAAQMIREGVLENPPVAVIFGFHVWPDTLGQVFSSPGPILAASD
                     SFEILVKGRSAQASHPQDGVDAIVLAAQIVNALQSIISRTIEPTDPALLTIGRIEGGT
                     QSDLLADRVRLTGIFRTLNDTTRRKIQRLMEAAVKGTTQAFGGDYSLAFKQSIPAVSN
                     HPELYASMLPVLNSVLADRPLQRLAPQMMADDFSFYGRKIPSLFFLLGVRNPRLSTAP
                     LESPYFNPDERSIAIGIKIISQIILDCLEQQSRLADSSQIVK"
     gene            15239..15736
                     /locus_tag="PKV64_01350"
     CDS             15239..15736
                     /locus_tag="PKV64_01350"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPN15754.1"
                     /translation="MNGENVLFVDSPLGMIELREKNGFLVGLNFRNRRPGKEGRVGSL
                     LSACACQLEEYFAGRRTVFEVPLALEGTAFQLEVWGALRGIPFGRTVSYGEIARMVGR
                     PAAARAVGAANGANPVSIIIPCHRVIGADGRLTGYGGGLNRKEWLLRHERGWAEAAAG
                     EGPDR"
     gene            complement(15753..17048)
                     /gene="eno"
                     /locus_tag="PKV64_01355"
     CDS             complement(15753..17048)
                     /gene="eno"
                     /locus_tag="PKV64_01355"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPN15755.1"
                     /translation="MSATTILSVKAREILDSRGNPTVSAKVVLKGGAVGTAAVPSGAS
                     TGTYEALELRDGDPARYLGKGVLKAVGHVNDEIAPRLVGKDALDTAALDRLLINLDGT
                     PAKTRLGANAILSVSLAAAEAAAAARTLPMYRWLRERDRYTLPVPMINILNGGAHADN
                     SVDIQEFMIVPAGRPTFAEAIRAGAEVFHNLKKILKKKGYGVSVGDEGGVAPNLGSNE
                     EALDCIVESIAQAGYRPGKDIWLALDCAASEFYGDGRYVFKKSDGASRTPAEMIAFYE
                     GLMAKYPIVSIEDGLAEDDWDGWTLMTETLGRKIQIVGDDIFVTTLSRLKEGIERRAA
                     NSILIKLNQIGSLTETIEAVEYAHAHDFTTVISHRSGETENTFIADLAVALDAGQIKT
                     GSVCRSERVAKYNRLMEIEEELGPKAVYAGTGAFSRFIK"
     gene            complement(17099..17389)
                     /locus_tag="PKV64_01360"
     CDS             complement(17099..17389)
                     /locus_tag="PKV64_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HPN15756.1"
                     /translation="MASDGKSSALEIAVSFLIGATTGFVLGILFAPASGKETRKRIGD
                     EFEKGKEKAVEGFEKLSKEAEKGIRTVREKTQEGIEVIKEFVDRKKGDQVKS"
     gene            complement(17379..17747)
                     /locus_tag="PKV64_01365"
     CDS             complement(17379..17747)
                     /locus_tag="PKV64_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15757.1"
                     /translation="MTLNQVLFLILTVAAVVAIVQLVRLFTQLRRTAIEGEKALGEIA
                     ELARHLKDLDLVIKEQVEELAKAIAASKKAAVNVAEASSLISAKFIRPGSKYLPLLLP
                     VAQFVWRRFGKKKKESHHGK"
     assembly_gap    17950..18049
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18284..19456)
                     /locus_tag="PKV64_01370"
     CDS             complement(18284..19456)
                     /locus_tag="PKV64_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPN15758.1"
                     /translation="MKGLKIFAVLLAVFLAAYFIPFGRSSVQNAVHEAFMMLQEYARM
                     HVLFCLVPAFFIAGAISVFVKKESVIRYLGPKANKFVAYSVASVSGTILAVCSCTVLP
                     LFAGIYTRGAGLGPATAFLYSGPAINVLAIIMTARILGWRLGLARVIGAVVFSVIIGL
                     LMHFFFQRKRDNPVEEAGFGLENGNVKERPLWKTALHIGSMIGILVFLNWAKSDPDVK
                     AWDFIFRLKWILSAAFAAALVWMTAAWFKKDELKEWLSSTWTFAKQILPLLLGGVMAA
                     GFLLGRPGHEGVIPGAVIAGLVGGNGLPANLFASVSGALMYFATLTEVPILQGLIGSG
                     MGQGPALALLLAGPALSLPSMIVIGKIMGTRKTAVYVSLVIFMATATGLIFGAFSR"
     gene            complement(19474..20781)
                     /locus_tag="PKV64_01375"
     CDS             complement(19474..20781)
                     /locus_tag="PKV64_01375"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPN15759.1"
                     /translation="MGEGGRNREPIGGISPNPAADPRPLRRYLKAGTRGRLDLPPGLI
                     KKLGASPGARLEVRARDGRFEIKPNIHSLSRLYIEPTSRCNLACRTCIRNTWKEPMGD
                     MSTALFGRLVSQLKDFCHLESVMFAGFGEPTAHADILRMIRAVKTLGLRVEITTNGTL
                     LDGPMIDGLRRAGLDMLWASFDSAGEKGFEDIRRGAKFEPIVAALKNLQAGNVRGRRK
                     IRVGISFVVMRRNVADLKRMDDLAREVGADKVLVSNILPYSEDMEKEMLCALTLSTAT
                     FSSAARTEISLPRMDVASHTREAILHLLQGYENLSLMGNPIFAETNFCRFVEDRTAFV
                     RWDGQVAPCLGLLHGYTTYLYGYERNIKPHGFGNVGQRTLGCIWRSRNYRRFREKIRN
                     FDFAPCHICGGCNLLESNDEDCLGNTFPSCGGCLWAQGLIQCP"
     gene            complement(20792..21850)
                     /gene="arsB"
                     /locus_tag="PKV64_01380"
     CDS             complement(20792..21850)
                     /gene="arsB"
                     /locus_tag="PKV64_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009225279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HPN15760.1"
                     /translation="MDDSRPKRLSFLDRYLTLWIFAAMAVGVGLGYFIPAVVGLINTF
                     QSGTTNIPIAVGLVLMMYPPLAKVKYEELGDVFRNVKVLFLSLVQNWIIGPLLMFALA
                     VLFLRDYPHYMYGLILIGLARCIAMVIVWNELAKGDTEYAAGLVALNSIFQVIFFSVY
                     AYVFITVLPGWFGLKGTAVDITIGEIARSVFIYLGIPFLAGIITRFTMLKLKGKTWYE
                     TKFIPKISPITLVALLFTIVVMFSLKGEYIVKIPLDVLRIAVPLVLYFLIMFLVSFFM
                     GRKVGADYSKTATLSFTAASNNFELAIAVAVAVFGLNSGEAFAAVIGPLIEVPVMIAL
                     VNVALRFRKRYFSGEAAA"
     gene            complement(21871..22557)
                     /locus_tag="PKV64_01385"
     CDS             complement(21871..22557)
                     /locus_tag="PKV64_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPN15761.1"
                     /translation="MNALFGDIGLILQNNPWLAIPAVFLGGVMTASNPCVLAMIPLMI
                     GLVAGRSESGGLKRTFLFSLFFVLGLAATFTALGLVSALLGRMFGDVGRFWKPALAVV
                     CLVMGANLLGLFHFNFSFPGIARFKGRGLLGSLALGLLFGLVSTPCAVPVLAVLLAFA
                     AEKGNVLFGGVLLFIYALGHSVLILIAGTSMGAARRMIESKGLKKASRGLQKAAGVII
                     ILVGAYLLVG"
     gene            complement(22573..23076)
                     /locus_tag="PKV64_01390"
     CDS             complement(22573..23076)
                     /locus_tag="PKV64_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPN15762.1"
                     /translation="MIPGRFPAARGKLLSALIPAALLILVSASASGVLGATPMDTTSA
                     AGSNPPPSQAENPAGPESLVIATGRLDIDFSKHLVTFIELGADKCIPCKAMQPIMREI
                     AAEYKGQVQVIFYDVWKNPEPGRKYGIQLIPTQVFIDKTGKEIFRHVGLFPKEELVAF
                     LKKQGVR"
     gene            complement(23073..23318)
                     /locus_tag="PKV64_01395"
     CDS             complement(23073..23318)
                     /locus_tag="PKV64_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011981102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPN15763.1"
                     /translation="MKIEILGMGCARCRDLEQRTRTALAETGLAAEVEKVDDIQRIMA
                     YKVIATPGLIIDGAVKAAGRIPSVDEIKKWISESLNK"
     gene            complement(23328..23753)
                     /locus_tag="PKV64_01400"
     CDS             complement(23328..23753)
                     /locus_tag="PKV64_01400"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPN15764.1"
                     /translation="MPDILRVFKSLADPTRLRIVRLLLQQDLCVCELMFVLEMAQSRV
                     SHQLRILRDASLVEDVRDGQWIIYRLPDNVRTTIAPLLALFKEDKKSAGAIHADRRRL
                     SACRKEGVRKKRCSPVESLKSKAEEKPASAKKRSAGDSI"
     gene            complement(24592..25443)
                     /locus_tag="PKV64_01405"
     CDS             complement(24592..25443)
                     /locus_tag="PKV64_01405"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPN15765.1"
                     /translation="MAELSILFLGISAIIALAFMYIPRNKINRWIAGKGLWGNVMGVL
                     FGAVTPFCACSTVPMTLGFLQAGVPFGAVMSFVVSSPLMDPLIFSLLVAFLGWKVALG
                     FFLLTSAFAIVFGVLLEKLGWANQIKNVRVKKIGPDPDKGVPEGFKNRLHAAVFKAWG
                     DFQSVFIYMIVGVGIGAAIYGYLPEDLLAKIAGAGHPLSVVIVALISMPLYLRVESAI
                     PIGLALMEKGASIGAVIALIISGAGIAIPELTMLAGIFKKKIIIGLSIIVFVSAVLSG
                     LIFNLLA"
     gene            25460..25939
                     /locus_tag="PKV64_01410"
     CDS             25460..25939
                     /locus_tag="PKV64_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15766.1"
                     /translation="MIASFSEFTVPSSFLPFPRSRFRRGDFSQGARDQSHVTCDFIDF
                     AAADGLKRPAEAADFATFHSGDDACSPRGNRHTADAAVMGGDIPPKISFLFETIDDSG
                     CSAFREIQGVPEVFDHKSGVFPGLFYRDELDERKIPAAFSVYSPASEKQCHLLQGIE"
     gene            complement(26105..26683)
                     /locus_tag="PKV64_01415"
     CDS             complement(26105..26683)
                     /locus_tag="PKV64_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15767.1"
                     /translation="MITYFFVKDARKDPRLFCSEPPGPMPARFSKTKEVWEAAKKKLM
                     LLPARTLRQEEALAKAVRLKDPAVTVLCGEAEEKHADLRFHFFLHKRRTQRILLLVGE
                     GILAPFTGLIAFLPGPNIVFYALALIMITHWQSLRGIRALLAKKHEYRVDPLLSEWEL
                     AVAEGREADYPGIIARLERAHGLKDMRKVLYG"
     gene            complement(26685..27614)
                     /locus_tag="PKV64_01420"
     CDS             complement(26685..27614)
                     /locus_tag="PKV64_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HPN15768.1"
                     /translation="MSARRLVLLPAILAAASIAAGLLAQERLIRDDRGREFRLEGRPS
                     RVISLAPNITEILFTLGAGSQVAGVTRYCDYPPEAAEKIRIGGFLDPDIERIRKAAPD
                     LIIAFRGNPWERIERLDDLGLRVFVLDIGFRLEEIRPLVEKIGTVVGRTSEAGILARA
                     LTDKAARVAAALSGVAVRPRVFLKLQGEGLWTCGRDSYFTDLVEKAGGTSVTAGLEKS
                     WLEYSVESLVRDDPDVIVVLAPSPAEFERTRAWLNSRPGLRATTAVRNGRILRLDENA
                     ASRFGPRLFDSLEELAGLLHPEKFPPGRTAAKE"
     gene            complement(27691..28401)
                     /locus_tag="PKV64_01425"
     CDS             complement(27691..28401)
                     /locus_tag="PKV64_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15769.1"
                     /translation="MDAKHLINRSPLRVLEDSTHGGPGPGGLAVLASPKGVGKTACLV
                     HIATDKLFQGKAVIHVSYSSRVDYIMTWYEDIFKEIVKTHRLRDSETVYDEIIKNRVI
                     MNFKQDGMRTGQVLRSLEAMITGGKFDADTIIVDGYNFGLAAPDDLDRFKDFARRMNV
                     SIWFSASLKETGGEMFGPDGTPVLLAPHAGRIDVLITLEAKSDAVEIRVVKDHDNPPA
                     GVLPIRLDPRTLLIAQEK"
     gene            complement(28416..30503)
                     /gene="fusA"
                     /locus_tag="PKV64_01430"
     CDS             complement(28416..30503)
                     /gene="fusA"
                     /locus_tag="PKV64_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPN15770.1"
                     /translation="MATTIERMRNIGISAHIDSGKTTLSERILYYCGKIHRVHEVKGK
                     DGVGATMDSMDLERERGITIASAATSVSWAEHRINIIDTPGHVDFTIEVERSLRVLDG
                     AVLVLCSVGGVQSQTITVDRQLRRYDVPRLAFINKCDRVGANPQKIRDQLEEKLKHNA
                     VLMQIPIGLEDRHQGVVDLVSMQAVLFEGDEIRRGEIPPDLREEAHRRRNDLLDAASM
                     FSDELTEAILEDRVTEELIHAAVRRGTIARKLTPVFMGSAYKNKGIQALLDGIVRYLP
                     DPSEVKNIAVDQSAPPDAPAEVVLSSDPDAPPVLLAFKLEDGPYGQLTYIRIYQGRIQ
                     KGTELQNARSGQRIKVGRLIRMHADHMEDIPEATAGDIAALFGIDCASGDTFTDPSCP
                     YTLTSIFVPEPVISLAVSAPDKRALDRLAKALNRFSKEDPTFRTFVDPESHETIIQGM
                     GELHLDVYVERMKREYNVEVATGKPQVAYREAITKKAEFDYTHKKQTGGAGQYGRVIG
                     YLEPLADGAYEFVNQVKGGRIPREFIPSCDKGCKTAVKKGGVIGFPVTGVRVVLTDGQ
                     YHEVDSSDNAFQAAALGAFRQAYAKAKPVILEPVMKVSVEGPSEFAGAVFGSINQRRG
                     VIVSSIEDGSFSRVDAEVPLSEMFGYSNQLRSLTQGKAEFTMEFLKYGRVPAAVAEEL
                     QKQHAEKRARETG"
     gene            30882..31313
                     /locus_tag="PKV64_01435"
     CDS             30882..31313
                     /locus_tag="PKV64_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPN15771.1"
                     /translation="MGLFGKSFAQKVDEAVAAVKAKVPEVKNLRAVLDGKVVTLEGDA
                     PDLNVKGRVVNEFNALVNTENTINKIRLAETPAPATAPPAAAPAEAEQVHVVVAGDTL
                     SALALKYYGKAGMYMKIFEANRDILANPDLIRVGQKLRIPK"
     gene            complement(31367..31945)
                     /locus_tag="PKV64_01440"
     CDS             complement(31367..31945)
                     /locus_tag="PKV64_01440"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HPN15772.1"
                     /translation="MSMAFLDEFMQAYGGQASRDLSAGLGLKKAAAAQIIPAVVPMIL
                     GGLKRQMETQGGAARIDHILNKYGSDSVLDDIAGAIGNRAGQAADAQLGGLLGSSGLQ
                     ATDMIARRFKLDPGVAAKIIPALAPLILGALSRKKNTSADGLGGIAALLDRDGDGSIL
                     DDVAGMLFQGSASPSSGGGLGKLLGGLFGGRR"
     gene            complement(31942..32082)
                     /locus_tag="PKV64_01445"
     CDS             complement(31942..32082)
                     /locus_tag="PKV64_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15773.1"
                     /translation="MKRTVLRSKTGKKLYAVRDAKGRFVDIQSYKRAHGQDIKRKSKK
                     EK"
     gene            32423..34912
                     /gene="leuS"
                     /locus_tag="PKV64_01450"
     CDS             32423..34912
                     /gene="leuS"
                     /locus_tag="PKV64_01450"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPN15774.1"
                     /translation="MSKDNAARDYDFQAIEARWQMEWNAAGAFAAAADPGRPKFYALE
                     MYPYPSGRIHMGHVRNYSIGDVIARFKMMRGYDVLHPIGWDALGMPAENAAIKSGIHP
                     QKWTLDNIAHMKAQLQRMGFAYDWSREVNTCLPEYYTWNQWIFLKMVERGLAYRRESW
                     VNWCPSCRTVLANEQAAGGVCWRCSSAVEQKKMEQWFLKITAYADELLSGHAQLGKWP
                     EHVLAMQKNWIGRSEGAYLNFDVPSLGKSIRVFTTRLDTIFGATFLVLSPEHPLVGDL
                     VRGPREAELRAWLAKTIAAARAKREIGEAEKEGVDTGVTAVNPFSGEPVPIWLANYVL
                     MEYGTGAIMAVPAHDGRDFDFARKYGLPIREVIVPAGSPRDPAAGLFEDRGVLVQSGP
                     YDGLTSDEAAKKMAGWAESQGVGQAAVTYRLRDWGISRQRYWGTPIPMIHCSACGIVP
                     VPYEDLPVRIPYEARFTGEAGSPLEKIPSFVNVACPKCGRPARRETDTMDTFVDSSWY
                     FFRYASPAEDRLPFRREAADHWLPVDLYIGGVEHAILHLIYSRFFTKLLRDLGLTALD
                     EPFPHYLAQGMVTMGGAAMSKSKGNVVDPDEMLGHYGADALRLFVLFAAPPEKEFAWN
                     EEGIEGCYRFVGRVWQLYREITALSAGEEPPAGGTVDPAAAARLRKKIHQTIRKVGED
                     IEIRFHLNTAVSAVMELANVLRKEKEALRNSAEGRALLAEGMEALILALAPFTPHVCE
                     EMWRGTGHETLVLRAPWPSFDPILAMEEKATIVVEINGKVRDKFEAEHDAAEEAVKEA
                     ALALPRIRELLEGKTVRKVVVIKGKIVNIVV"
     gene            35063..35590
                     /locus_tag="PKV64_01455"
     CDS             35063..35590
                     /locus_tag="PKV64_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HPN15775.1"
                     /translation="MFPASFRAGLAVGLAFACASCGYMVRGSGRFLADRNISSVYIPI
                     FKNLTTRYEVDVKLTEAVISEFISRGRVAVVPGSESADGILEGEIQGFIVEPIAYTGA
                     QGSADRYSITIRASVVLKDIKKNTVLFSNPAFVYRGEYEVPEGMDFESQETEALDQIA
                     KLFAKNLVVFIVEGF"
     gene            35594..36676
                     /gene="holA"
                     /locus_tag="PKV64_01460"
     CDS             35594..36676
                     /gene="holA"
                     /locus_tag="PKV64_01460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPN15776.1"
                     /translation="MAGSFLPVPDRPEDVRPAYFFCGEDLYEARGFVAELRRRLAGPG
                     EAAVEVETFILADRRWTEVLDAARNMPFFFSPWRLLIVEGKGAGQEDLSAGEAAAFRD
                     YFASPAPQTTIIVLFEGKIRKTRPLGKSIAALPGPLARIEEIAPRKDREIKSLTAEKF
                     ESLGKQATPEAVERILDIAGGEIARIDSEVEKLAVYLGPRRTVEADDVAALCSARSFD
                     NWELTAALEAGDTDKALIIIESLFENGEAPEMILAVLSGFFRTALLAKAGLREGRDPK
                     EVFREAKPSISERFGGFYQRMLGEYLALLGRIKDADLLRWLEELEDMDRRLKSTAVAP
                     KEMIQAFVVGYGRKAPGRRITSPGRR"
     gene            complement(36654..36932)
                     /gene="rpsT"
                     /locus_tag="PKV64_01465"
     CDS             complement(36654..36932)
                     /gene="rpsT"
                     /locus_tag="PKV64_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPN15777.1"
                     /translation="MAKHASAERQHRASLRRNAVNTKNKSSLRTQVKKLRVLIKAGDK
                     SEAQKALPSVNKIIDQTVKKGAIHKNTGSRYKSRLSRQITKISDAPAK"
     gene            37007..37480
                     /gene="dtd"
                     /locus_tag="PKV64_01470"
     CDS             37007..37480
                     /gene="dtd"
                     /locus_tag="PKV64_01470"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPN15778.1"
                     /translation="MRVVLQRVREASVQTGGEVVSRIGPGLCLLVGVEKGDAEADADR
                     LARKAASLRVFSDAEGRMNLDVAEAGGAILAVSQFTLAASVRKGRRPSFDGAEEPARA
                     AALFDRFVAALRSTGLEVSTGVFQAEMLVRLVNDGPVTFVLDSAGGPAPAGKPLS"
     gene            complement(37473..38060)
                     /locus_tag="PKV64_01475"
     CDS             complement(37473..38060)
                     /locus_tag="PKV64_01475"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPN15779.1"
                     /translation="MTKFLRRPEPASPDVVEQALRDIESLLPAETQTGDTQAFTNALL
                     KKLDIFRDRAKKMRMMRALAHLDAPWIPSVFLELLGDSIEEIRDLAVRELSRRDNWPA
                     AALYERLDRPPWYAKSACLRVLAEKKDRAAAVHIKRLIDDPNVDVRRAAALALGAIGG
                     PAARLLLVRLTRDKSRYVRAAAEEMLDRICDFKFS"
     gene            complement(38063..39283)
                     /locus_tag="PKV64_01480"
     CDS             complement(38063..39283)
                     /locus_tag="PKV64_01480"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPN15780.1"
                     /translation="MAVKAPHAVNVERALEAVRAGRLIIITDDEDRENEGDFMVAAEK
                     TTPEVVNFMATYGRGLICLPLTRERLQQLQLPLMVQENTARFQTAFTVTIDAVEGITT
                     GISAFDRAATIRAAIDPKTKPSDLAKPGHILPLQAKEGGVLARAGQTEASVDLARMAG
                     LTPAGVICEIMNPDGTMARRPELERLSREHGIPILTIADLIQYRMQTECLVRKVSEAD
                     LPTKYGTFRIFIFEDEIHGETHVALVKGDISGDRPVLVRAHSQCLTGDAFGSSRCDCG
                     EQLHGAMTMIEKEGSGVVLYILNHEGRGIGLAAKIQAYAIQDQGADTVEANTRLGFKP
                     DQRDYGIGAQILVALGIKRLRLITNNPRKFIGLSGYGLEIVERVPLEIAPNAINADYL
                     KTKKDKMGHILEMV"
     gene            complement(39293..39883)
                     /locus_tag="PKV64_01485"
     CDS             complement(39293..39883)
                     /locus_tag="PKV64_01485"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPN15781.1"
                     /translation="MFTGIIQARGIFRGFRENGREMIVESPEAAGRIAPGQSLAVDGV
                     CLTLVRKEGHRLFFNTVRETLERTNFSLRRPGETLNLEYPLTLADPLGGHLVSGHVDF
                     TSKIKRVSSRSSGRRLAVILPAAFRPFVVAQGSIAINGVSLTVAAVGPGSFDVELIPE
                     TLGRTNLADLRAGKAVHVECDMLGKYVYNFLHFHKR"
     gene            complement(39883..40989)
                     /gene="ribD"
                     /locus_tag="PKV64_01490"
     CDS             complement(39883..40989)
                     /gene="ribD"
                     /locus_tag="PKV64_01490"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPN15782.1"
                     /translation="MIDERDLAYLDMAEALAVQALGRTSPNPHVGAVVVRRGRIVGTG
                     FHEAAGREHAETAALRRAGKNARGATLYLTLEPCVHWGRTPPCAESVLTSGIVRAVIA
                     GLDPNPVIRGRGVRFLRTAGLAVDVAGRPERKSGLNEAHARYIVDRRPFVTLKSALSL
                     DGKLATRTGESAWISSLEARRYAHFLRSENDAVLVGAQTVRHDDPRLTVRPPGRRGKP
                     VLRVVLDPGLRTPPEARLFEDPSGGPVLLYARAGNRDWEARKARLLRRGAEVAELPGE
                     GPRLDLRRVLSDLGSREIAALLVEGGGRTAAGFLDEGLVDKVVLAVAPILIGGADAVA
                     LFAGRGADRLAAAPRLSRVSSFRLGGDLILEGYI"
     gene            complement(40986..41879)
                     /gene="ftsY"
                     /locus_tag="PKV64_01495"
     CDS             complement(40986..41879)
                     /gene="ftsY"
                     /locus_tag="PKV64_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPN15783.1"
                     /translation="MLGRLWEGLAKTRQAWKDRFGKVFGPGRPREEILETLSESLILA
                     DAGVPVAEKITAAVRNRTKKNDDAETLESVLKSELIELLSRRQIRDEGPGLSGVILLV
                     GVNGGGKTTTAAKIAQRAAAEGKRVLLVAADTFRAAAQEQLAIWGRRLGVPVHQGRPG
                     SDPAAVVFDAAREFLAGGYDLMIVDTAGRIHTNANLMSELDKIKRIVSREIDGRPVET
                     WLILDAALGQNALVQAREFRKCAGLSGLVLTKLDGTARGGAVLAIADEYNLPIRLVGT
                     GEGAEDMEDFDPAAFVEALMS"
     gene            41977..43671
                     /gene="recJ"
                     /locus_tag="PKV64_01500"
     CDS             41977..43671
                     /gene="recJ"
                     /locus_tag="PKV64_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPN15784.1"
                     /translation="MSETAWSIAPPSTAAPALAAALGLPPVLGQILANRGFTDAAGAR
                     LFLEAGLEDIPDPFLLSGMDAAVARIEKAIREKETILIFGDYDVDGVLATVMLHKALS
                     ALGGKVDCFIPERLKDGYGIKDGHAEAVVARGARLVVSVDCGIKSAGFVRAAAAAGVD
                     VIITDHHLPGPERPAALAVLDPVLPEAGYPDRNLAGVGVAFKLIQALYQRAGRAGVLP
                     HYLKLVAIGTVADVVDLRGENRILVRRGLAELARVSNPGLRSLMEICGLTGRPVTVGD
                     VGFRLGPRINAAGRMGRTELALRLFFSKSAAEAAAVAGELDVLNRRRQETEDSILADA
                     EGRVRAGGLAETHKILILGDEKWHRGIIGIVASRLKEIFHRPVVLFSYDDGCAFGSGR
                     SIPGFSLIDCLEEARPFFLSYGGHRQAVGCTLRRESVPAFKEAMNRAVDARLTFDDLR
                     PSIAIDSRLEFTDIGADLWRGLALLEPHGVGNPRPVFLTEGAVVAAEPRKLKDRHVKF
                     LLRKDGRTFEAIGWNRGAWAGEFPAGSRIDGVFTFQTSAYLGEERPYLALEDLRAG"
     gene            43680..45638
                     /locus_tag="PKV64_01505"
     CDS             43680..45638
                     /locus_tag="PKV64_01505"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HPN15785.1"
                     /translation="MAHPSSRSRAKTAVRVLLIGFLAVLLAVVFVFLSRRSDRPAAIR
                     PETRPLTTQKVDVKENIHFVKDTGGRNILDVQAARNFTDAVGLYHLKGPEGEKGGGVR
                     VESRARDGQLKFAAFAEEVIYDADWTRLVFRGGVEIRLDDLTVKGASFTYHKNRDAVV
                     TNVPAVFEGRRFRGDCRRATYNVTDEYFVFSGGITLVTAPWDDDPVPVIITGKSMTYA
                     RQPRTGEIRGDVRIAHGRSRGRCNDVFFAQFPDKPEFRLFDFHGNVKWEADERAVPAA
                     VPAKPKAGEASPADDELIFFQGGRQRMEAGSMMFVPYGTEGWARYGSMQGGARVDILG
                     EAGRQTTLEARETVFFYDEGWALQAFRLQTRARIRGEAEGRMRLLEAPTINYGADSGL
                     LAANGTAAEPARTISGGREITAEWIALNLQTKNLDARGGVKIVSHPEAGNAGDAALFA
                     SGRTTFMTAGLAHYEGGERQFRLGEGARLWQGRESLEGRDVLIFEARGDISASGNVRS
                     VFFHRDQKKNEDQRVEVTGGRMARQSKKGNILFLDSCSLTVGEIVLKAGRLILEPGGG
                     ENGEKYRRIIADQERVTVTQGANTARGDRAVYDLSEDVIVLTGNTVLEDLAKGVIHKD
                     GKLTFHPSDGRILIENKDAERSTTIIKS"
     gene            45642..46355
                     /gene="lptB"
                     /locus_tag="PKV64_01510"
     CDS             45642..46355
                     /gene="lptB"
                     /locus_tag="PKV64_01510"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPN15786.1"
                     /translation="MKSSLNASHLSKSYDGKKVVDDISIEIQRGEIVGLLGPNGAGKT
                     TTFSMILGLVARDAGRIYLNGEDITDDPMYLRARKGICLLPQDPSSFRRLTAEDNLLA
                     IARPAGRRRGDARRKLREYGLESLARAKAYTLSGGERRRLEIARAMMTSPEFILLDEP
                     FTGIDPIAIQDLQRIVRNMKDDGLGVLITDHNVQDTLRIVDRAAIIDKGRIVEEGSPE
                     AVVASPEVRRRYLGEDFRV"
     gene            46379..47836
                     /gene="rpoN"
                     /locus_tag="PKV64_01515"
     CDS             46379..47836
                     /gene="rpoN"
                     /locus_tag="PKV64_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPN15787.1"
                     /translation="MALKQRLDQRQVQKLILAPALQQAIKLLPLTNLELIEVIDEELS
                     ANPLLELEEESPEVKSVEETPAAPSASEDLPAGTAGEAEESLAEDREFESYFQEYIDD
                     GFRAGPAEPPEAPNIENMISQSASLGDHLTWQANLTFFDPLDREIAERIIGNINDDGY
                     LTSSLDEIGLSAGAPIERVRDVQAKILQFDPVGCGGLDLKECLLAQMDNLQIKEEAAR
                     AMISEHLPLLEKSDFGQIAKLLNLPLAEVKVHLEIIKQLDPAPGRKYGEDRTHFVVPD
                     ITVSKESGEFKVALNDEGLPRLRINAYYRRLLARALKENPEAYRYLKDKMKKALWFLR
                     SLDQRDQTIYKVAKAIVDRQKDFFEKGLDGLQPLTLMEIAQEIGVHESTVGRVVANKY
                     MITPRGTYSLKYFFHKSLQGGFGEEISSLRVKDRLRRLIESEDKSRPLSDMEIEEILT
                     RENFPIARRTVAKYRQQLDILPSHIRKRKFLMEES"
     gene            47836..48363
                     /gene="raiA"
                     /locus_tag="PKV64_01520"
     CDS             47836..48363
                     /gene="raiA"
                     /locus_tag="PKV64_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPN15788.1"
                     /translation="MNVHFTARHAAIPPDLKTYAEKRLAGLARFLDRATEADVVLSAE
                     KSRQRVEIQVRGKQGRIRVAADGHDAAAVMNEAFDLLDVKLKKERAKFREKKRRGGRE
                     TAAAAAGETAAGLPPRVVRADYFSGKPMSVGDAVVEFNLRKKEVLMFRMEGEEDRWAV
                     LFRRKNGTFGLVRPE"
     gene            48360..48923
                     /locus_tag="PKV64_01525"
     CDS             48360..48923
                     /locus_tag="PKV64_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15789.1"
                     /translation="MNAVRSAAPAVVSGGLLRMFGLGVLIIGDSGVGKSESAIELIAR
                     GHRFITDDVVEVRRRADGRIIGRAPRLSRHFMELRGLGLINVRYIFGPKSILRESTID
                     LVIRLKKWSRKKEYDRLGLRFPEDHLILGTRVPQLNIPVAPGRNIATLIEVACVSHLL
                     LRRGRRAPDQMLRRLNRAFAAGRTGKP"
     gene            48920..49783
                     /gene="rapZ"
                     /locus_tag="PKV64_01530"
     CDS             48920..49783
                     /gene="rapZ"
                     /locus_tag="PKV64_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPN15790.1"
                     /translation="MTHRPSRFLIITGLSGSGKTVASRFLEDLGFFCVDNLPAKLIPS
                     FVDLRRRKSEVLERVALVMDIREPGFLTDFPKEWAKIKKIQGARLLFFDATDGAIVQR
                     FSESRRPHPLARGRSVLDAVRLERKRLASIIELADDVINTSSMTSAQLRDVLSRRFFR
                     ADKQPFIIRIISFGYKHGIPLDADLVFDARFLPNPFYEDDLREKPGTNARVREFVLRR
                     PETKDFLAELGRFLNFLLPRFEAEGKSQLVIGVGCTGGYHRSVVIAAALRDVLRGRKY
                     DIKVFHRDIAK"
     gene            49780..50187
                     /locus_tag="PKV64_01535"
     CDS             49780..50187
                     /locus_tag="PKV64_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15791.1"
                     /translation="MIGGLIVMHGKLGEELLNVLTIILGETANIEAISIGWYDDVEES
                     KKKIASSLKRVDQKNGVVIFTDMFGGTPSNLSFTFQKDGSVEIITGANLPMLIKFISL
                     QRGMSLRDVARKVVEQGRKNIHLASAILDGKKS"
     gene            50189..50458
                     /locus_tag="PKV64_01540"
     CDS             50189..50458
                     /locus_tag="PKV64_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012070121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPN15792.1"
                     /translation="MIEHVVRIKNRLGLHARAAVKFVNLANRFTADVRIEKDGSDVDG
                     KSILGILTLAATQGTEITLRLNGDDEAAALKAIVDLIDGRFGEKE"
     gene            50473..52242
                     /gene="ptsP"
                     /locus_tag="PKV64_01545"
     CDS             50473..52242
                     /gene="ptsP"
                     /locus_tag="PKV64_01545"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPN15793.1"
                     /translation="MDYLRLRGIGVSPGIVMGPVVLADRTLFSSRKVYIPPYRVADEI
                     ERLNEALRRTGDEITTIKDQVRAMAGDEHAFIFEAHLLILQDPALTSGLERLIREENI
                     RTESALSEVHEYYRRVFDAIADEYIRQRKADVTDVLTRIYRNLDAGEKLSVSDDLRGK
                     ILVAHDLLPSEAAIRLSQGGVLAVVLDMGGPTSHAAILARSLDIPTVVGLRDITQQVS
                     AGDELIVDGTDGEVIVNPPLMIRREFQSKKAKYEAYRKELRRIATWTPVTLDGIRFVP
                     MANIELPEEVGHALSYGAEGVGLFRSEFIYLQRATLPTEEEHFAIYDRLARDAFPRPV
                     YIRTIDIGGEKTLPQLKIEAEPNPALGLRAIRFSLQNRELFRVQIRAILRASVRKNVR
                     VIIPMITEMEEIGEIRVLFREAREELAAQGLGFDPDVPLGIMIEVPAAAAIADLLARE
                     VDFFSIGTNDLLQYFLAVDRSNEYVSYLYKPLHPAALRLLKHVIVSADCQGKDVALCG
                     EMAADPLAAVVLLGLGLRTFSMNPIFIPRIKKALRAVEVRVVRRVVQKAMNLRSAQEV
                     EEFIIEKLLFKYPDAFLMTSPSS"
     gene            52302..52613
                     /locus_tag="PKV64_01550"
     CDS             52302..52613
                     /locus_tag="PKV64_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15794.1"
                     /translation="MEFSRLLVGLAVCLWAFVIGFSIVAFGQILLAVREIALNTRKEE
                     GKSHHGHYAILLVMAKINTVLGWIVVVGGLAAGIYVIVFGVPAFTGLTTPVVDSAYLR
                     P"
     gene            complement(52598..52975)
                     /locus_tag="PKV64_01555"
     CDS             complement(52598..52975)
                     /locus_tag="PKV64_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HPN15795.1"
                     /translation="MSPSEDERNFRSRIIEDHRPWGKFRAFPHEQAGSIKIITVNPGG
                     SLSLQSHRRRAEFWVALDEGLEVTVGDRVWRPAADEEIYIPREARHRVRGVGPRPARI
                     MEIWLGDSDENDIVRYEDVYGRK"
     gene            complement(52962..53435)
                     /gene="ssb"
                     /locus_tag="PKV64_01560"
     CDS             complement(52962..53435)
                     /gene="ssb"
                     /locus_tag="PKV64_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPN15796.1"
                     /translation="MRDVNSLNKVILIGRIGQRPELRHFPQSDRALARFTLATNERSF
                     NPQTSQASIRTEWHSIVAWAKLAEFCDRFLEVGKQILVEGKIRTRSWQDRDGNKRKTT
                     EIEAFNIVLLGPRDAQPRGTEIDTPYREPRPDAPSPDFPSDEEPPASLGEDDVPF"
     gene            complement(53432..54286)
                     /gene="bamD"
                     /locus_tag="PKV64_01565"
     CDS             complement(53432..54286)
                     /gene="bamD"
                     /locus_tag="PKV64_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPN15797.1"
                     /translation="MKRSWPAVLLIAGLSLSAAAQDRSKKAYELMYEDIQLLKIQTQR
                     IEKKIDQAGADLKGLGDIVRDLAAQFKDFRIFQARNQEGWSEIPGQVRSIQEQLAQIE
                     NRLLQLAADLESAKTPAGETTAAPEAGGKPKPEEKAGAARKPAAEPPAATVPPSGLSA
                     QEAYQAAMADFSKGNLDLAVDGFALYREQFGASPLADNALYMIGECRFSQRKFPAAIE
                     AFDDLILTYPMSDKIAAAYLKKGFALAEMKKKTEALAVLRLLLSKYPLEEEARQAQQK
                     IKELQEAP"
     gene            complement(54292..54849)
                     /gene="pal"
                     /locus_tag="PKV64_01570"
     CDS             complement(54292..54849)
                     /gene="pal"
                     /locus_tag="PKV64_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HPN15798.1"
                     /translation="MKKTIVLALVCLLAISLGLGVSCKAKTKPTPPPPPQVKEQPVVE
                     QVTETAPAEAAPLSEEELFLRKTLDQINQERALKNIRFDYDKYFVRDDAKGILEANAA
                     WMKKFQTAKILIEGHCDERGTEEYNLALGEKRAKSTMDYLLSLGIPAERMRIISYGKS
                     QPLDMGHDETAWANNRRAQFLVIEK"
     gene            complement(55281..57323)
                     /locus_tag="PKV64_01575"
     CDS             complement(55281..57323)
                     /locus_tag="PKV64_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15799.1"
                     /translation="MGFKGATAWTTVLSRSSPETWFRRLSGGLIFLALVQVLTIALSL
                     AHIVISQAAALAVLAAAAAGAWGFDRLFRRFPETGPNETAAPSPLAPILRGVIPILLA
                     LLGLTVIVAYFHPYLFGDCLIYHLPAVHYWSEKGFVHWVAPQPLKPFFTDLFINGYPK
                     AAELMGFVVVQATGISGLVHLLNAWFLAIGISGTALIASSLGASAEASLTAGLLFALA
                     PVHIQQLNGTYVDSAFASAVVALFAVLSRLAAGTRGRFAAWDEVAVFGAGAGLVLGLK
                     SSGILPAGVAGAAAVIVLGLRRRRLPAESRRAANLRTWAWIPVVFLISALVGGYWYAR
                     NLAHTGNPAYPLTLKIGDPGRSAKSVDFILNKAANTPPVIRNWPGWKQVAYVWMQGGP
                     AHWPYSVCSASGRLGGLGLLWLLGCLPAVILACLTGKKSGPPPGLRRARITLLLCAAL
                     VFLLTPMHWWARYTVWPLGIGLPFLVLVLDKFWESRGRKWIASWAILLAAVFLAETAA
                     GIMWLAGASRIDWYGGQTAFPAERKIGFDRLTWSRPFMTIDETRRGAAYRRMKGDFEP
                     VLLGPLNQQRVLSPPMTAMFGMVCDPLGRRRVFVTDFPRLQGLRKLRRFLRENKIRYA
                     IWDASAAQIPPFDRLCLRKENDGTYRYFIFEEPEDPAGKKETISPPLSAETVKTAE"
     gene            complement(57408..58709)
                     /locus_tag="PKV64_01580"
     CDS             complement(57408..58709)
                     /locus_tag="PKV64_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HPN15800.1"
                     /translation="MKKNVLVTIILFLSAAGGASAQQEFNMRITEGMPAVAFALPPFV
                     AAPAAKDAAAEIHSVLEADIRYTRIFELLPKSYYGYIRPLTDPNSPEFKDWESIQANV
                     LLTGRVEAEPEGGIIFSWVLYDVKSKRTIIAPRRFQYKDKNLRYLAHATADEIMKAYG
                     EKPIFTSKIAFVSNRDRNEEIYLMDYDGANQTRLIYNQVNDSNPAWDPGGKRLAYSSW
                     QNLVVGVYILDVFEKARARTPVSLKGQNYTPAWSPDGKKLAFSSSMNGNHEIYVADIE
                     SNPTRVGRIKQLTFNPAVDIAPSWSPNGREIVFTSDRSGTTQIYIMDAEGGNVRRISF
                     GANHHDAPAWSPNGDKIVYVARVDNAFDLYLYNIRSGQISKLTEIYGRNESPSWSPDG
                     RHILFTSNVSGRNQIHAIDVDGRNFRKLTSMGENKLADWSN"
     gene            complement(58706..59530)
                     /locus_tag="PKV64_01585"
     CDS             complement(58706..59530)
                     /locus_tag="PKV64_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPN15801.1"
                     /translation="MTWSAAGSDRAFKRALIISAAGHVILFSFIILNPSLPKKPSPKG
                     VIHYISMGMVGGGGGGRGGGGDGRLPGGGNKAAGGTTAVSQSKPESLRDLTVPEKVVP
                     KTESRLRYPVDDKTKRKAAAEKRASISKPGPGGKAGASGSAAGTGGGGEGGTGLTIGP
                     GGAGSGGGFGEGFGDGLAEFPYAYYLLRVRDEITVNWFPGSIDPGPNNVLQTLVYFRI
                     YRNGSISTIEIKQSSGLRAFDLIAVRAVANAAHFPPLPDDYDGEYLGINLLFEHAR"
     gene            complement(59527..59970)
                     /locus_tag="PKV64_01590"
     CDS             complement(59527..59970)
                     /locus_tag="PKV64_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HPN15802.1"
                     /translation="MVLPKLPTVRAKRDVGTALSEINVTPFVDVMLVLLVIFMVTAPM
                     MQSGIGVDLPTAETDTAPAEEGLSITLRPDQSVYIGDSIININLLERQLQAAFYGKSK
                     KVVYLRADKSLPFGFVMDVLDIVKKTGVEVVGLMTRPEEKKAGRK"
     gene            complement(59957..>60237)
                     /locus_tag="PKV64_01595"
     CDS             complement(59957..>60237)
                     /locus_tag="PKV64_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPN15803.1"
                     /translation="TGSVSPFIGLFGTVWGIMDTFIGIGVTGSTSLVSVAPGIAEALI
                     ATAVGLFAAIPAVMAYNMFLHQLKDQITDMEDFSLELLSIAERLYGSP"
BASE COUNT        11385 a        18033 c        19116 g        11503 t
ORIGIN      
        1 cgaggagctc ttctaccgcc tgaacgtcat ctccgtcgac atgccgcccc tccgtcgaag
       61 gcccgatgac atccccctgc tgatccagca tttccttcgg aagcattgcg agcggatggg
      121 ccggaaaatc aagcgcttga ctcccgaagt caccaccgcc ttgacgaagt actcctggcc
      181 gggaaacgtc cgcgagctgg aaaacatcat cgagaggctt tgcgcggtcg aggagcggga
      241 aacgatcacg gcggagagtc tcccggacga tgtcgtcgaa gccggacggg tgccggtggg
      301 aggggcgccg gttcttttgc ccggtttcga tctcgaagcc cacctggatc aagtggccca
      361 agcttatctc aaggaagcga gcgaaaaagc cgccggcaac atgcgccgga tggcggagct
      421 tttaggcgtc agctaccggt ctcttcgcta cttgatgaaa aaatatcaaa tcagaaggcc
      481 gggcgccgcg gaatagagga aaacgcaagc aaagcctacg ggcttcttga cagggaacgg
      541 ggggaggagc ggggcaataa acgacacttc gttgacaaat aatgtcaata ttccgtcttt
      601 tttcatttcc tttgtgacat atattattta tattcatata tttagaatct acgcattatt
      661 ggcatacatt ttgctgataa tattatgcaa agaaaaattt ttcgaattcc ggcggcttcg
      721 aaaaaccgcc aggaagcagg aggtttcaat gttcaagaat cggaaagggt tcaccctgat
      781 cgagctgttg atcgtcgtgg cgatcatcgg catcctggcg gccctcctca tccccaacgc
      841 gatgacggcc cttcagaagg ccaaagtccg cgggacacag aaggacatca actcgatcgc
      901 caccggcatc gccgactaca tcacggacaa ggctgtcccc ttcaccgcca acggggcgat
      961 ggactcgacc gtgaaaacgt cgctgtctcc cctgtatttg aaggttctgc cggagacgga
     1021 ccagtggggt caggcgttca tgatttacac cgggaccaac gtcagcggcc agtacggcat
     1081 ctcttccgcc gccgtggacg atttcctggt cgcctcttac ggccgggaca aaaccatcga
     1141 gggctggacg tataacgata ccacgcccga tgccggcttg tattcgatcg gagccacggc
     1201 tgacttcgac aaggacctga tcaactacaa cggcgcgttc atccgcggac cgcgggcggg
     1261 cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncccaatg ccatgaccgc
     1381 ccttcagaaa gccaaggtcc ggggaacgca gaaagacatc agcgcgatcg ccacggccat
     1441 cgccggcttc atctcggaca agacgtttcc gcccccggcc aacggctcga tcgacatcgc
     1501 ggtcaacacg gcgctatcac cgttttatct caaggtcctc ccgaacatgg atcagtgggg
     1561 ccagccgttc atgattttca cgggggataa ctgcacccaa tacggaatct ccgcccccgg
     1621 ggcggatgat ttcttggtca tttcctacgg ccgcgacaag accctcgaat cctggaccta
     1681 taatccggcc acgcccgaca gcggcttgta ctccatcggc gcggcggccg acttcgacaa
     1741 ggacctgatc aacttcaacg gcgcgttcat ccgcggccct cgggccgggg ccgtcggcac
     1801 ctgattttcc tccgctctct ttttttctcg ccccgcggat tggggtataa tcggcccgat
     1861 gattattcgg acggccgttc ttcttctttt ttatgccggg atcatccttt gtctcattcc
     1921 cgggcttctt ttcgccgccg ggctcggtct tcgagaaacc ttggtccgca tcggaaagtg
     1981 ggccatggga gcgagccggg ccgtcctcgg cctccgggtc gacgttttcg gccgggttcc
     2041 tgaatcggaa gacggaccgt tcgtctacat gtccaatcac gtcagccttt tagacggacc
     2101 gcttcttttc cggttgattc ggggacccgt cagggtcatc atgaagacgt ctcttttccg
     2161 catccccgtc gtcggattgg cgatgaaaat catcggattc atcccggtcg accggagcgg
     2221 aacccaaggc ggccgggcgg ccgtcagccg ggccgcggcc gccataaagg agaaaggatg
     2281 ttcctttctg atctttcccg agggaacccg gagcctgaac ggccgtcttc agacgctccg
     2341 ccggggcgga tttttcttgg cccgagaagc gggcgttcca atcgcgccgg tttcgatcga
     2401 gggaacgttt gaaatcatgc cgaaagggag attggtgccg ggaaagggct cgatccgggt
     2461 ctttttccat ccgccggtcg ccgatcccga gccgggatcg agcgacttcg aagcctggat
     2521 gagcgccgtc cgggaaaggc tgtcgtcttt tccggcctga gaaggaggag agtcatggac
     2581 catcaacgca tcgctcaaga ttggagccgg atgctcgacg aatttttcgt cgcccggggg
     2641 gagacgttcg ccggagcgat cgaggcctgc gtcagtgctt tgaaaaaagg ccggaaaatc
     2701 ctcgccttcg gcaacggcgg aagcgcggcc gaggcccagc atttcgtcgc cgagctcgtc
     2761 aacaaattcc tcaagccccg gccggccctg cgggccgtga cgctctcgac ggacacctcg
     2821 gtcctgacct gcatcgggaa cgacgtctcc tacgaggccg tgttcagccg ccagatcgag
     2881 gccctcggcg atccgggcga catcgtcctg gccttgacga cgagcggcaa ctcccccaat
     2941 gttttgaccg ccttgaaaac ggccaaggag cagggcttgc tgacgatcgt cctgacgggc
     3001 cggggcggcg gcaagagcgc ccccctggcc gaccttctcc tggacgttcc ctcgaccgaa
     3061 accccgcgga tccaggagat ccacctcttc cttttgcatg tcctggccgg agaaatcgaa
     3121 gagaggatgt atccgtcttc ttgaggcccg gtccgtccgg ccggaatccg tcttacccgc
     3181 cgatcgtctc cttgatcttg tccacgccgg ccgccccgga gttgaagaga aatgttcgga
     3241 gattcggaag gagacggcgg aagagatcct tttcgatctc gatccgctcc tccgtcgagg
     3301 caagaaaaga cgcgttgaaa aacggcttgg ggacggccgc agccgatttc ttgatccccg
     3361 gcgtttctcc cctttcaaga atccggaggg catcctcggg attcaactcc tcgagcgccg
     3421 gcgagaccga gtcgtttcgg aggacaaaca gccagcggag ctgcgtccgg cggacggccg
     3481 cccccggacc gagccaggaa ggatccagca gggcgcgggc gtccttggcc gccttgtagc
     3541 agaacggccc gccccggtcg agccggcaat cctcggcccg gaggcattcg gcattggggc
     3601 agtcttcctt ccggatgacg acgttttcgc atttgctccg gtcgaagagt cgggccaggc
     3661 gcccgaacag ctcgaccgtg ttcgtcggca gccaggcctt gcgctcgatc gcctcggcct
     3721 gaaccgcctt gccggccggc ctggccgaaa tcatgtcggc ggatacgaag cggaagcggg
     3781 gatcccggag aaggctgaag aaaagatccg ttttctttgt tccggccggg ccgacgagaa
     3841 tcactccgcc gccctcccgg tcgaccgcca ttccccggat caggcaggag ccgaagtggc
     3901 gtccggccac gtcgccggcc aggcccaggg ccaggcttcg gacgggagca taggaatcgc
     3961 agttgacgac gaccccggtc ttcgtttcgg tgttgaagaa ggcccgggga tctcggccgg
     4021 ccaagccgtc gactgcgtac aagacgccgt gcggctcgat gccggcctcc gcgggggccg
     4081 gcgtccagtt ttccacccac atctcgtaaa cgtggcggag gttggtcttc aggcggagaa
     4141 tgactccggc gacggccgcg ttccattcga agagaacatc ggcggccagc cgggcttccg
     4201 tctcggccgt caggccgtcc cgttgattca gcggcaggcg gggatcgacc ggaaccggcc
     4261 cgcgcacccg ggtgacgacg ttgttcaagg cgacgcgctt ggctttgcgg gccggcttga
     4321 gccgggccaa ggcctcggcg atccggttga gtcccttttc caggttctcc agggacgtgg
     4381 cgtaggaaat ccggatatga tcgtcggctc cgaacgcgtc gccgggaacg atcgccactt
     4441 gggcttcccg caggagataa tacgccatcc cgtaggagtt gcggatgggc gtcccgttga
     4501 attccagatc gtaaaacgac cggacgttcg gaaaaagata aaaagcgccc tgaggctcga
     4561 aacaggagac gccggggatg gttcggagct tcatcaggga gtagttgcgc cggcgctcga
     4621 actcggcccg catccgggcg acgtcggctt gggatccggc taaggcctcg acgctggccc
     4681 gctgggagat ggacgcggga tgtgaagtgg cgtggctttg aatgcggctc atggccgcga
     4741 tcaagggagc cggcccggcg gcgtatccga tccgccagcc ggtcatggca taggctttgg
     4801 aaacaccgtt gatcacgacc gtttttttct tgaggtcgtc cccaagggag gcgatgcttc
     4861 ggtatttaaa tctgtcatag acgaggcagg agtagatttc gtcggaaacg acacagagat
     4921 cctccccgcg gatgacctcg gccagggctt caagctgggc gagagtataa gccgcccccg
     4981 tgggattcga cggggaattg agaagcagcg ccttggtcgc cggcgtaatg cgggccttga
     5041 gctcctcggc cgtcaggata aagccgtttt cctcctttgt gggaacgaaa acaggcttcc
     5101 ccttggccaa attcacgaga tggggatagg aaacccagta cggggcgggg atgatgactt
     5161 cctcgtcgtc gttgatcagg gcttggaagg cgaggaacag cgagcttttc gctccgcagt
     5221 tggcgatgac ctcgttggga gcgtaggtca ggccgtaatc ttctttcagc cggccgatga
     5281 tggcttgctt gagggccgga gagccctcgt tttccgtgta cttggtgtag ttatcctgaa
     5341 tcgccttgat tcccgcggcc ttgacggatt cggaggtcgg aaaatcgggc tcgccgatgc
     5401 tcaggtcaac gacgtcgagg ccttcggctt tcatcgcctt ggctttggcg ctgaccttga
     5461 acgtcggcga ggagccgatg cggttggctt tgtcggaaat catgatttcc cctttcccaa
     5521 gcggccgcgg gggctatttt tcgctcttct tttcggcggt cagcttgtcc ttgaggaatt
     5581 tctttaaatc ggctccggtc agcgattgaa ggatttcagg cacctgggcc aggatttggg
     5641 ccgtttgggc cgagatcttg gagacgccga gatccttgtc gctgttgacc aggacgatct
     5701 tatcgatttt cgaaagcggc tcggccacgc ttttcaccac atccggcagg acgttcatgt
     5761 acatttcgag gaccgaggcc tcttgataat ggttcatcgc ctgagcccgc ttcgtcaagg
     5821 cctccgcctc ggccgcgccc ttctccttga tccgggcggc ttcggcttgg ctttcaagct
     5881 tgaccgcctc agccttgccc cgggcttcgg cctggatgcg gtattcctcg gcttcggcct
     5941 ccgcccgaat ttggtacttc cgggcgtcgg ccggcttgag aacgttggaa tccagttctt
     6001 tctcccggcg ctggatttcc agctcctcga tcttgatggc ctgttctttc tcgaccaagc
     6061 ggaccttgct ttcctcccgc ttcaattcct gcgagaggcg gtggcgttcg agctcatagg
     6121 aaaagtcggc ctgggctttt ttctggttca cccaggtcag cgactcggat tttttggctt
     6181 cgttttccca ttgggccttg gcgatctggg cttcagcctg aagccgggcc acctcgcctt
     6241 ccttccgggc ttgggacgag cggatgatgg cctctttctc ggtctcggcc tcggccacgg
     6301 ccgcgtcccg cttggccgcc gaaatcaagg gcttggacag ggcgtcgatg tatccctggg
     6361 tatcaccgaa ttccttgagg gcgaaggaca ggaggacgag tcccattttg ccgaagtcgt
     6421 accggacggc ttcgccgacg cggcggttga actcggatcg gccgcggtaa atctcctcga
     6481 ccgtcatcgt cccgatgact tcccgcatct tcccctcgag gacgttgacg gccacgtccc
     6541 ggactccttc tttccccatc cccaggaatt gctcggcggc cgtgtgaatc gacgcctcgg
     6601 aggagtcgat tttcacctgg gccgcggcgt cggccacgat gggaacgccg ccgtgggtca
     6661 gaacctccgg cgtgcggatg gagatcggaa tgacgtccat cgggaggatg tcgacttttt
     6721 ccgtgaacgg gcggacgaat gtccccccgc cgatccggac gcggaaaccg accttcttcg
     6781 ctgtcccgtc gggcagggcg acggttcttt tccgcccgga aatgatcaag gcttcgttcg
     6841 gaccgacctt ccggtattga cgggcgaaca ataagacgaa aagaacgatc agggcgagga
     6901 cggctccggc caggatgaga atggcgacga gactcatggc aacctcccta aaggacgttt
     6961 gcagcgagta tggcggtctc cataactgat taaaaacgca tgatataaag cgcgtttgcg
     7021 gttgtcaaga cggaagaaaa agccgcggat tcagcgccca taggccatga acataaggct
     7081 tttctttttc agaacgtctt tttgagcctg gatgacttcg acgatcccgg cgtcggccat
     7141 cttcagaaga gagtctagct gatgacgcga gaacggaacc ctttcggccg ttgcttggac
     7201 ttcaagcagc tgcccgcgat cggtttcgac gacgttcata tccgtctccg cggccgcgtc
     7261 ctcgctgtag tccaagtcgc aaaggacgtc gccgccgacg attccgacgg agatcgcccc
     7321 gaccaggtgg cggagcggca gaaccggaag gatcttcgtg tccagcagct tcttcagggc
     7381 cagggccagg gcgacggagg ccgcgttgat ggaagcgacg cgcgttcctc cgtcggcttg
     7441 gaggacgtcg cagtcgatga tgatctgccg gtcgcccaag gcccggaggt cggtgaccgc
     7501 ccgcagcgtc cggccgatca aacgctggat ttcctggctc cgccccgacg ggtatcccat
     7561 ctggcgctcg cggggggttc gtttctcggt ggcccggggg agcatcgcgt attcggccgt
     7621 cacccagccc tggcccgtcc ctttcaaaaa cggagggact ttttcctcga tcgtggccgc
     7681 ggccaccacc cgggtgcgtc ccatttcgat gagggccgag ccgtcggcga cttcgataaa
     7741 atcgggggtg attttaaccg ggcggatctg atcggccgcc cggcttccgg accgcccggg
     7801 gcggagatta ggatttttca tcatggggcc tcgactgcca gcgtttatga atccacaacc
     7861 aggaatccgg atcgcggcgg acggcgtctt cgatcatctt agtgcagacc gccgtgattt
     7921 tcaacacgtc caccgcctcg tctcccgtga gagggagatc gacgggcggg ccgaagatca
     7981 gccggtagcg gtctcccggg gcgggaacgc aaaacatcgg gacgatcgga gctcccgtcc
     8041 ggagatgaaa aacggccaac gccggggtcg tggccgccgg gatcccgaag aaatccacga
     8101 aaaccgcctc ccggcggagg acgttctggt cgatgacaat gcccacggcg cccttccgcc
     8161 cgagggctct cagcaccgga cgccccgccc cgaatttgtt caagacggtt gctccccgcc
     8221 gggcccggac gctttccaac tcccggtcga tgagcggatt gtccagcgct cggacaatga
     8281 catggaaggg aacgcggcgg gcgatgggcg tgatgaccat ctcccaattg ccgaagtggg
     8341 ccgtgaaaag gagaacgccc cgtccgcggg cgagggcggt ctcgaggttt tccttcccct
     8401 ggacgtcgat cagcgcttcg acccggcccg ggggccaggg ggcggtcttc aggatgtcga
     8461 aaagggaacg gacgaaattc atcatcgacc ggcgggccgt ccgccggcgg tcttcatggg
     8521 aaagggatcg gccgaaagcc cggctcaggt tgtcgagggc gatgcgccgg cgcctggaat
     8581 ccaagtgata gacgagggct cccaaggctt cgctgaaaag aagaacgatc cgccgcggga
     8641 gccgggcgac gaatgaggcg aaaaggcgat acagaacata agaaaggtgg attccgacgg
     8701 acgaggaatc cgggcggcgc ttaggcatga ccggcctttc ccggaaaaac ggcggcctcg
     8761 agacattcga aaaactcgtc cggcaggtcg agcccgatgc ggagaacggt cagccccggc
     8821 ccctcccagg ggagctcccc tgcttcgaat ttgaccgcgt ctttttccgt ggtcaggacc
     8881 atctcgggat ccgtccggga caccgcggcc gcgatccggg cgcgggacgc cgccggatac
     8941 aaatgatgat cggggaaggt catccggccg acgacagccg ctccgagcga ttccagctgg
     9001 gagaagaagc ggcggggacg ggcgatgccg gcgaatgcca ggacgcgttt tccggaaagg
     9061 gcttcgagcg gaacgcccgt ttgtccgtcc gctgaaaaaa gcccgcgggg gacggcggcg
     9121 tattcgaaaa ccttcaagcg cgggaagcgc cgccgatagg cctcaaaccg ttccggcgag
     9181 gtcccccggg ggaggagaag aacatcggct cgggccaaag cccggtcgcc ttcccttcga
     9241 ggcgacgaag agcgcggatc atgaagaacg atgtcgagat ctcgggcgag gtcgagacgc
     9301 tggaacgcgt cgtcgagaac gcagaaatcg aaaccgaggg acgccgccgc gcggcaggac
     9361 gcgaggcggc cgcggccgac gaaaacaccg gctcggggat agcggcgggc gactagaacc
     9421 ggctcatcgc cggcttctcg gaaaccgccg aaaaacgttt ttccatccga cacgacgccg
     9481 ccggaccgtt cccaagcgcc gcggtatccg cggctgacca gggcgggcct ccatcccttc
     9541 cggagaaaat gagcgatgat ttcgcaggcc agcggcgttt tcccctctcc tccaagggtg
     9601 agattgccga tcgaaaggat cggaagaggg gcgcgggccg gcgttttccg ccctcggcgg
     9661 taccgggcgg ccttgccggc gcagacgggg cgggaaacca gggaatagac ccggagaagg
     9721 attctcattg cggcagaata tatcatagaa cggctgtaat gccgatatct cccccggaca
     9781 gacacccttg ctccggcggt cccccttttg agtttacttg gcttgtcgaa tcatctataa
     9841 ttcggccaag tgtatctctc attctaggag gcgatcaatg aaacgtcggt tcaatttttg
     9901 gatattcgcc ctggcggcgg tcctggtcct gaccggctgc caaaagaaga ccccgatcaa
     9961 gggcgtttcc ctcgacgtcg ttttcgccga gtcgcccctg acggacaacc tgatcaccga
    10021 ggtcacatac acgtggaaga ccggcaagga tttcgagaaa ttcagccggg actaccacgt
    10081 cttcgttcat ttctggcaca acgacaatct cattctccag gacgatcacg ctccggcgcc
    10141 gcccacgtcc tcctgggaac cggacaagac ctacaccatc acccggcgga tcttcatccc
    10201 caaattcatc gatgaattca atccccagtt caagggcgag gagacactcc gtttctccgt
    10261 cggcctggtc aatcccttcg accggagcgg cgatcccccc cgcgaggtcc tgatccagaa
    10321 gctcaaagtc gttccgccgc cgatcggcac gcccgagatc atctacgaga gcggctggta
    10381 cgacctcgag atcgatcaga acgcgcccct caagcagtgg cgctggacgg ccaaggaagc
    10441 caagtgcatc atcgataatc cgcgccggga cgccgtcctg gtcattaaag gagcggcgaa
    10501 tatcgccgac gtcaaggatc aaaagatcat ctttaaaatc aacgatttga cggttgatga
    10561 gttcattccg ggcgaaagcc ttttcgagaa gacctaccgg atccaaaagg aaatgctggg
    10621 ggacaaggat gaattcatcc tgtccatcgg ggccaaccct tcgtttattc cggcggatat
    10681 ccgaccggct tccaaggacg cgcgcgaact cgggcttcag gtttccttta tttatttccg
    10741 gtgaggagcg gaaccgccct cacttgcccg gattcttgga atcgaagtcc tcgagctctt
    10801 tcttgagccg ggcttcctcg gccgcggccg cctgaatatc caggttcgtt tgggcgatgt
    10861 cctgctgcac cttggccttg gattccatcg tgttgaaaga gaagaactgc tgctggaggg
    10921 cccgcatctt taacgtgacg tactccaggc gttccttggc ccggtcgtac ttgtcctgaa
    10981 gatcggcccg gaggccctcg acgtcaacgg cggtttcgac gacgagggct tgggaaggtt
    11041 cggccgccac ggccgagggt ttcatttcag gctcggccgg agctccctcg gctgcggccg
    11101 tctgttctcc gtcctcggtc cgggtttcgc tgatggccgc tttttttgtc gccttggtca
    11161 gatcggtgtt ggtgaccgtg accgagggct tgcctttaag ggaagcccgg cgttcctttt
    11221 cctttcgggc ggcggcggcc agatcctggg ccgaggcgga ggccgccatc cctaaaagaa
    11281 caaccgcgat aaccgctttt ctgaacttca tccttctttt ctaataataa aacgagagac
    11341 aagtcaagtc aaatgctcga tttcggcaaa aggccggcgg tcatcgccgg gacgtccgga
    11401 tggagagttc ccggccgtcg cttcggatct cgacggctcc ctgtcgatct gtgcccagaa
    11461 cccgagctcc gcatttcccg aaacggtcca ggacggcggg gtggggaagt cccatccggt
    11521 tgccgcgccc ggcggaaacg acgatgacct ccggacggac ggcctccagg aattcctccg
    11581 tgctcgagga atcgcttccg tgatgaggcg ccttgagaac ctgggcccgg acatcgaggc
    11641 cggcggccag gatttccctc tcgacgtccc ggccgatatc ccccgtcagg agaaaggccg
    11701 tcgagccgta gatcaggcgg aggacgatcg acgagtcgtt gccgggggct ttcgccccgg
    11761 aggtttcggc cggggctaaa acggcgatgt cgactcctcc gaggttatga ttgaatccgc
    11821 ggccgactcc gcggaccgga acgccgccga gttcttcccg gagggcgcgg taacgcgcgt
    11881 cttcgggagc cggcgttcct tcccagaatt ccccgatgga aaaattcctg gcgacggccg
    11941 ccaggccgtt gagatggtcc gggtgaggat gggtcaagac gagacggtcg attttcttga
    12001 ttcccttgtt ccagagaaaa ggggatacga cgtgctcccc gatgtcgaat gttcccgtcg
    12061 gcagtccgcc gccgtcgacg agcattttct ccgaaccggg gaattcgacg agaacggatt
    12121 cgccttgccc gacgtcgatc acggtgacgg tcaggtcctt gacgtccgcc gggaacggat
    12181 gcgtcgtcaa gacgatgagg gcggcggcga agccggcgaa ggaaaaccgg cggaggcgga
    12241 cgaaccgggc gggaaggaga agaaggagaa gaaagacata gtaggcggcc acgaccggtc
    12301 cgggcggcgt cggaacgcgg ctggagagga acggcaggcc gtccaaaagc cgggtcgagt
    12361 ccagcaaaat ccggaggagg aaggaaagac cgctggccgc gatcccggcg gcaaagggaa
    12421 aaacgaagga cagggggaga aaaacgtatc cggtggccat cacgacggtc accaggggaa
    12481 tgccgatcag gttgaggatc aaccccgaga aaatcacccg gtgaaacgtc gccgcgatga
    12541 tcggcaggac ggccatctgg gcggcgaccg aaagggcgaa gagctcctcg attttgaagg
    12601 gcaggcgggg aagggcggcc cggatcgaag ggaagaagag gatcaggccg agggtggcgg
    12661 cgaacgtgag ctggaagccc gcgtcgaaaa gctggaaggg ccggacgaag agaatgccca
    12721 gagcgctgag ggaaatcgtg ttgaggagat ggacatcctt ccaaaagagc ttgccgatga
    12781 tgaaggcggc ggccatgacg accgcccgga tgacggaagc ccgcccctcg acgagaaagc
    12841 cgtagaacac caggaggagg agaagaagga gataggaagg ccgttcggcg atccggaacg
    12901 cccggaaaag gaagaaaagg agagcggaga cgatggcgat atgggcgccg gaaatggcga
    12961 acagatggaa caagcccgat ttctggaggt ctcgggtcgt cgcctcgtcc atccgggagc
    13021 gttctccgag gacgagcgcc tcgaaaacgg ccccttcggg ggagattcct ccggggcgtc
    13081 cgggatcggc gaaggaccgc tcgatgaggt ccaggcaggt ccggcgcagc cgcgaaatcc
    13141 accggaacgg aacgctccgg cggcccgggc cgaggtgttc aaccagcgag gggcttttcg
    13201 tcgagccgag ggcatggaga tcccgcgttt tcatatacgc aactgaaaac ggcgccttga
    13261 aattccaata ttctgagggg gggacgacct gggccgatag gcggacccgg tcgccggcga
    13321 tgagccgggg cagcttccct cccgtcgcgg aatgcttcac cgcgacccgg atccgtccgg
    13381 tcatgggacg gtccgctccc cggagggaaa ccgattcaac ccgcagatag agatcgtcgc
    13441 ggtccaagcc gggcgctggg gaaagggaga gcgttcccgt gaaatcgaag tattcgccgg
    13501 tcggcaggct ccgcagctcg ttgctttcat atcgcgcttc gaaagcggaa cccaggccgg
    13561 cgcccaggcc ggcggccgcg gtcaggagga ggacgaaggc cgccgcagtt ttccgccggg
    13621 cgaacaggat ccaggcggcg gccaggacga cggccgtcgc ggccgccgcg gcggcggggg
    13681 aaagagaaag aaaaccggcg gcggcgatgc ccgcggacac ggcgatcgcc aggaaaagaa
    13741 cgggataagc catcgcccgg ctatttgacg atttggcttg aatcggccag ccggctttgc
    13801 tgctccaggc agtccagaat gatctggctg atgatcttga tgccgatcgc gatgctccgc
    13861 tcgtccgggt tgaaatacgg gctttcgagc ggagcggtcg aaagcctcgg gttcctcacg
    13921 ccgagaagaa aaaagagcga cgggattttc cttccgtaga aggagaaatc atcggccatc
    13981 atctgggggg cgagccgctg aagggggcgg tcggcgagga cgctgttgag gaccgggagc
    14041 atcgaggcgt aaagctcggg atggttggag acggccggga tggattgctt gaacgccagg
    14101 gaataatccc cgccgaaggc ctgtgtcgtc cccttgaccg cggcttccat cagccgctgg
    14161 atctttcgcc gggtcgtgtc gttcaacgtc cggaatatcc ccgtcaggcg cacgcgatcg
    14221 gctaaaagat cggattgagt ccccccctcg atccggccga tcgtaagcag ggccgggtcc
    14281 gtgggctcga tggtccggct gatgatcgat tgaagggcat tgacgatctg ggcggccaaa
    14341 acgatcgcgt cgacgccgtc ctgggggtgg gaagcttggg cgctccggcc tttgaccagg
    14401 atttcgaagg agtcggaagc ggccaggatg gggccgggcg aggagaacac ttggcccagc
    14461 gtgtccggcc aaacatggaa tccgaaaatg accgcgaccg gaggattctc gaggactcct
    14521 tcccggatca tctgggcggc cccgctctcg ggatcctcgg aggcgggatc gggccccggt
    14581 tggaagatga acttgactcc gcctttcatc cgatcccgga gggcgttgag gacataggcg
    14641 gatccgaggg ctacggccac gtggatgtca tggccgtggg cgtgcatcac gccgccgttg
    14701 accgaccgga acggcacatc ggccagctcc tggatgggta cggcgtccat gtcggcccgg
    14761 agggcgacga tcgggccggg ctgggctccg cggagcaatc cgacgatgcc gcctctggcc
    14821 actccggttt tcacttcgaa tccgagggcg gccaggcggg cggcgacgat cttggccgtt
    14881 tcctgctccc gccccggcag ttgcggatcc atatggatca accgccggat cttgaccatc
    14941 tcggccgcga ttttttccgt ttcccggtcg atgtcggcgg ccgttttttt atcgacggcg
    15001 gaaaaagaaa gggacggaca aagaaaagca aataatagag ttattgccgg aaagagcttg
    15061 cgcatgaccg gctctcatct taatctggag agccgcggat tgtcaaattc ggaccctctt
    15121 ttccggaaaa accggggatg gtccgcttct tgtccgaggt tgggaaatga aaatcggacg
    15181 gaggcgaagg attgaacggc gggtcggagt ttgatagtct cggcggcgga aggtcaaaat
    15241 gaacggagag aacgttctgt tcgtggattc gccgcttgga atgatcgagc ttcgggaaaa
    15301 gaacggcttt cttgtcggcc tgaatttccg gaatcggcgt cccggaaaag agggccgggt
    15361 cggttccctt ctctccgcct gcgcctgtca gcttgaggaa tacttcgccg gccggcggac
    15421 cgtgttcgag gttcccctgg ccttggaggg cacggccttt cagctcgagg tctggggcgc
    15481 gcttcgggga atcccattcg gccggaccgt gtcctacgga gagatcgccc ggatggtcgg
    15541 gcggccggcc gccgcccggg ccgtgggggc ggccaacggg gccaatcccg tctccatcat
    15601 catcccctgc caccgggtga tcggcgccga cgggcgtctg accggatacg gcggcgggct
    15661 gaaccgcaag gaatggcttc tccggcacga gcggggctgg gccgaggcgg cggccgggga
    15721 aggccccgac cgctgatccc ggaccggttc tcttacttga tgaaccggct gaacgcgccg
    15781 gtgcccgcgt aaaccgcctt cgggccgagt tcttcctcga tttccatcaa ccggttgtac
    15841 ttggcgaccc gctcgctgcg gcagacggag ccggttttga tctgcccggc gtccagggcg
    15901 acggccaaat cggcgatgaa ggtgttttcg gtctcgccgg agcgatggga aatgaccgtg
    15961 gtgaaatcat gggcgtgggc gtattccacg gcctcgatcg tctcggtgag gcttccgatc
    16021 tggttgagct tgatgagaat cgaattggcc gcccggcgct cgatgccttc cttcagccgg
    16081 gacagggtgg tgacgaagat gtcgtcgccg acgatctgga ttttccgccc gagggtttcg
    16141 gtcatcagcg tccagccgtc ccagtcgtcc tcggccaatc cgtcctcgat ggagacgatc
    16201 ggatacttgg ccatcaggcc ttcataaaaa gcgatcatct cggccggcgt ccgggacgct
    16261 ccgtccgatt ttttaaagac gtagcggccg tcgccgtaga attccgaggc ggcgcagtcg
    16321 agggccagcc agatgtcttt ccccggccgg tatccggcct gggcgatgct ttccacgatg
    16381 caatccagcg cttcttcatt ggacccgagg ttgggggcga cgccgccctc gtctccgacg
    16441 gagacgccgt agcctttctt cttcaggatc tttttcagat tgtggaacac ctcggctccg
    16501 gcccggatgg cctcggcgaa ggtcggccgc ccggccggga cgatcatgaa ctcctgaatg
    16561 tcgacgctgt tatcggcgtg ggccccgccg ttgagaatgt tgatcatcgg gacggggagc
    16621 gtgtagcgat cccgttcccg gagccaccgg tacatcggca gggtgcgggc ggcggcggcg
    16681 gcttcggccg cggccagcga tacggaaaga atcgcgttgg ccccgagacg ggtcttcgcg
    16741 ggcgtgccgt ccaggttgat cagaagacgg tcgagggcgg ccgtatcgag ggcatccttc
    16801 ccgaccaggc ggggcgcgat ttcgtcattg acgtggccga cggccttgag gacgcccttg
    16861 ccgaggtagc gggcgggatc gccgtcgcgg agctccaagg cttcataagt tccggtggag
    16921 gcgcccgaag gaacggcggc cgttcccacg gcgcctccct tcagaacaac tttggcggag
    16981 acagtcggat tccctcggga atcaaggatt tcacgggctt tgacggacag aatcgttgtt
    17041 gcggacatgg accaatcctt taaaatgaaa tatacggatt aataaatcgg ccggccggtc
    17101 aggatttgac ctggtcgcct tttttccggt cgacgaattc tttgatgacc tcaatccctt
    17161 cctgcgtttt ttcccggacg gtgcgaatgc ctttttccgc ctctttggag agcttctcga
    17221 atccctcgac ggccttttcc ttgccttttt cgaattcgtc cccgattctc ttgcgcgttt
    17281 ctttgccgga cgcaggggcg aacagaattc cgagaacaaa tccggtcgtc gccccgatca
    17341 gaaacgagac ggcgatttcc agggcgctgc ttttgccatc acttgccatg gtgactctcc
    17401 ttttttttct tgccgaaccg gcgccagaca aactgggcga cggggaggag gaggggaagg
    17461 tatttggaac cgggccggat gaatttggcc gagatcaggg aggaggcctc ggcgacattg
    17521 accgcggcct tcttggacgc ggcgatggcc ttggccagct cttccacctg ctccttgatg
    17581 acgaggtcga gatccttcaa atgccgagcc agctcggcga tctcccccag ggctttttcc
    17641 ccctcgatcg ccgtccggcg caactgcgta aacaggcgaa cgagctgaac gatcgccacg
    17701 acggcggcga ccgtgagaat gagaaaaagc acttggttga gggtcaaggt catttcctct
    17761 ataaaatact ggaaaatcag ggggttgtca atgtgaacgg cttccgtaga ttcatgcgac
    17821 cggggaaaaa tgacggggcg tgagatgtcc gaaggctcgg cggggacgaa cgaagcgatc
    17881 gattcaattc cattcctcca accggcgatt ggggacttcc gccgccgacc cgatgtcctc
    17941 ctcctcctcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ctccaaccgg
    18061 cgattgggga cttccgccgc cgacccgatg tcctcctcct cctcaaactc tcctcgtatc
    18121 caagccatgt ttttctcgga ctggaggagg cggccgtcgc ttttcatttc cctcccggcc
    18181 ggtcccggaa aggacgttcc ggggccgctt ttgcagctag gcccccggtt caaacgtctt
    18241 tttccaaaac gttcgtttcc caggctctcc tctcgttttt ggactatcga gagaaggcgc
    18301 cgaaaatcaa tccggtcgcg gtcgccatga agatgacaag gctgacgtag accgcggttt
    18361 tccttgttcc catgattttt ccgatgacga tcatgctggg aagcgaaaga gcgggcccgg
    18421 ccagcagaag ggccaaagcc ggcccctgtc ccatccccga tccgatgagc ccttggagga
    18481 tggggacttc ggtcagggtg gcgaagtaca tgagggcccc ggagacggag gcgaacaggt
    18541 tggccggaag gccgtttccg ccgaccagac cggcaatgac cgcgccgggg atgacgccct
    18601 catggccggg gcgccctaaa agaaatccgg ccgccatgac gccccccagg aggagaggta
    18661 ggatttgttt ggcgaatgtc caggtcgagg acagccattc cttgagctcg tcttttttga
    18721 accaggcggc cgtcatccag acgagagccg cggcgaaggc cgcggagaga atccatttca
    18781 gccggaaaat gaagtcccag gctttgacgt cgggatcgct tttggcccaa ttcaagaaaa
    18841 ccaggattcc gatcatcgac ccgatgtgaa gggcggtttt ccaaagagga cgttctttga
    18901 cgtttccgtt ttccaggccg aagccggctt cttcgacagg gttatccctc ttcctctgga
    18961 agaaaaaatg cattagaagg ccgatgatga ccgaaaaaac gacggctcca atgacccggg
    19021 ccaggccgag cctccagccg agaatccggg cggtcatgat gatggccagg acgttgatcg
    19081 ccgggccgga gtagagaaac gccgtggccg gcccgagtcc cgccccccga gtgtaaatgc
    19141 cggcgaaaag cgggagaacc gtgcaggaac aaaccgccag aatggttccc gagaccgagg
    19201 cgaccgagta agccacgaac ttattcgcct tgggtccgag ataacggatg acggattctt
    19261 tcttgacgaa aacggaaatc gccccggcga taaagaaagc cgggacgaga cagaacaaaa
    19321 catgcatccg ggcgtattcc tggagcatca tgaatgcttc atggacggcg ttctggacgg
    19381 aggagcggcc gaaggggatg aaataagcgg ccaggaatac agccaggaga acggcgaata
    19441 ttttcagtcc tttcattgaa gaattccttt cactcaaggg cactggatga ggccctgggc
    19501 ccagaggcac cccccgcaag aagggaatgt gttgcccaga cagtcttcgt cgttggattc
    19561 gagcaggttg catccaccgc agatatggca gggggcgaag tcaaagttcc ggattttttc
    19621 ccggaatctc cggtaattcc gcgaacgcca aatgcatccc agcgttctct gcccgacatt
    19681 tccgaatcca tgcggcttga tgtttcgttc gtatccataa agataggtcg tatagccgtg
    19741 aagaagcccc aggcaggggg cgacttgccc gtcccagcgg acaaaagccg tccggtcttc
    19801 gacgaagcgg cagaagttcg tttcggcgaa aatgggattg cccatcaggc tcaagttctc
    19861 gtaaccctgg aggagatgga gaatggcttc ccgggtgtgg gaggcgacgt ccatccgggg
    19921 cagggagatt tccgtcctgg ccgccgaaga aaaggtcgcc gtcgacaggg tcagggcgca
    19981 gagcatctcc ttttccatgt cttctgaata gggcaggatg ttgctgacca ggactttatc
    20041 cgcgccgact tcccgggcca ggtcgtccat tcgtttcaga tcggccacgt tccgccgcat
    20101 gacgacaaac gagatgccga cccggatttt acggcggccg cggacgtttc cggcctggag
    20161 gtttttcaaa gcggcgacga tcggttcgaa tttcgccccc cggcggatgt cctcgaatcc
    20221 cttttccccg gccgaatcga aagacgccca gagcatatcc agcccggccc gccgaaggcc
    20281 gtcgatcatg gggccgtcaa gcagagtccc gttggtggtg atttcgactc gaagcccgag
    20341 ggtcttgacc gcccggatca tccggaggat gtccgcgtgg gcggtcggct cgccgaatcc
    20401 ggcgaacatc accgattcca ggtggcaaaa atccttcaat tgggagacca accgcccgaa
    20461 caaggccgtg gacatgtcgc ccatcggttc tttccaggtg ttacgaatgc aggtccggca
    20521 ggccaggttg cagcgggacg tcggctcaat ataaagccgg gacaggctgt ggatattcgg
    20581 cttgatctca aaacggccgt cgcgggcccg gacttccaaa cgggcgccgg gggaggctcc
    20641 cagctttttt atcaatccgg gcggaagatc caggcgtccg cgggttccgg ccttgagata
    20701 ccggcgcaag gggcggggat ccgcggctgg atttggagaa attccgccga tcggctcccg
    20761 gttgcggcct ccttctccca tgtcggcttc ctcaggcggc cgcttccccg gagaaatatc
    20821 ttttccggaa ccgaagggcg acgttgacca gggcgatcat gaccgggacc tcgatcagcg
    20881 gcccgatcac cgcggcgaag gcctcgccgg agttgagccc gaaaacggcc acggccacgg
    20941 cgatggccag ctcgaagttg ttgctggccg cggtgaagga cagggtggcg gtcttggaat
    21001 aatccgctcc gactttccgg cccatgaaaa acgagacgag gaacatgatc aggaaataga
    21061 ggaccagggg aacggcgatg cgcaggacgt cgagcgggat tttgacgatg tactcgccct
    21121 tgagcgagaa catgacaacg atggtgaaga gaagggcgac cagggtgatc gggctgattt
    21181 tggggatgaa tttcgtttcg taccacgtct tccccttgag cttcagcatc gtgaaccggg
    21241 tgatgatgcc ggccagaaaa ggaatcccca ggtagatgaa cacgcttcgg gcgatttccc
    21301 cgatcgtgat gtctacggcc gtccctttca gtccgaacca gccggggagg acggtgatga
    21361 agacataagc ataaaccgag aaaaagatca cctgaaaaat ggaattcaaa gcaaccaggc
    21421 cggcggcgta ttccgtgtcg cccttggcca gctcgttcca gacgatgacc atggcgatgc
    21481 agcgggccag cccgataagg atcaagccgt acatgtagtg gggataatcc cggagaaaaa
    21541 ggacggccag ggcgaacatg agaagcggcc cgatgatcca gttttggacg agcgaaagga
    21601 acagaacctt gacgttccgg aaaacgtcgc cgagctcctc gtatttcacc ttggccagag
    21661 gcgggtacat catcaggacc aagccgacgg cgatcgggat gttcgtcgtc ccggactgaa
    21721 acgtgttgat gaggcccacg acggccggga tgaaatatcc gagcccgacg ccgacggcca
    21781 tggccgcgaa gatccacagg gtgagatacc ggtccagaaa tgaaagccgc ttcgggcgcg
    21841 aatcatccat tgccatctcc ttttcgcgtt ttatccgacg agaagatatg ccccgacgag
    21901 aatgatgatg acgccggccg ctttttgaag gccgcggctc gcttttttca atcccttgga
    21961 ctcgatcatc cttcttgccg ctcccatgga tgttccggcg atgagaatca agacggaatg
    22021 gccgagggcg taaatgaaga ggagaacgcc gccgaagagg acattcccct tctcggccgc
    22081 aaaagccagg agaacggcca gaacggggac ggcgcaaggc gtggaaacaa ggccgaatag
    22141 aagcccgagg gccaacgagc cgagaagccc cctgccttta aatcgagcta ttccggggaa
    22201 ggagaaattg aaatgaaata gcccgaggag attcgccccc atgaccagac agacaacggc
    22261 cagggcgggc ttccagaagc gcccgacgtc gccgaacatc cggcccagaa gggccgaaac
    22321 gagccccagg gcggtgaacg tcgcggccag tccgaggaca aagaacaacg aaaacaaaaa
    22381 cgttcttttg aggccgcctg attcgctccg gcccgcgacc aggccgatca tcaggggaat
    22441 catggccagg acgcaggggt tggaagcggt catcactccg ccgaggaaca ccgcggggat
    22501 cgccagccaa ggattattct ggaggatcag gccgatatcg ccgaaaagcg cgttcatttc
    22561 gcgggtcgac cttcagcgga ctccctgttt ttttagaaaa gccaccagct cctccttggg
    22621 gaacaggccg acatggcgga agatctcttt tcccgtcttg tcgataaaga cctgggtagg
    22681 gatgagctgg atgccgtatt ttcggccggg ttcgggattt ttccagacgt cgtaaaatat
    22741 cacctgaacc tggcccttgt actcggcggc gatctccctc atgatcggct gcatcgcctt
    22801 gcaggggatg catttgtcgg ctccaagctc gatgaacgtc accaggtgct tggaaaaatc
    22861 gatatcgagg cgcccggtgg cgatcacgag gctttccggg ccggccggat tttcggcctg
    22921 ggacggcggc ggattcgagc ccgccgcgga ggtcgtgtcc atcggggtcg cgccaaggac
    22981 gccgctggcg gaggccgaaa ccaggatcaa gagagcggcc gggatcaggg cggacaagag
    23041 cttgccgcgc gcggcgggga atcttccggg gatcatttgt tcaaactctc cgagatccat
    23101 ttttttattt cgtcgacgga aggaatgcgc ccggcggcct tgaccgcgcc gtcaatgatc
    23161 agcccgggag tggcgatgac tttatacgcc atgattctct gaatgtcgtc gactttctcc
    23221 acttccgccg cgaggcccgt ttcggccaga gccgtgcgcg tccgctgttc gaggtcgcgg
    23281 catcgggcgc aacccatacc caagatttcg attttcatga cgatctccta aatagagtcg
    23341 ccggcggatc gctttttcgc cgacgccggc ttttcttccg ccttcgattt gagggactcg
    23401 acaggggaac atcttttctt tcgaactcct tctttccggc aagcggaaag ccgccggcgg
    23461 tccgcatgaa ttgctccggc ggatttttta tcctctttga aaagggcgag aaggggggcg
    23521 atcgtcgtcc ggacgttgtc cggaagacga tagatgatcc attgcccgtc ccggacatct
    23581 tccacgagac tggcgtcccg gaggatcctg agttgatggg aaacgcggga ttgagccatc
    23641 tccaggacaa acatgagctc gcagacgcag aggtcctgct gaaggagcag ccggacgatc
    23701 cgcaagcggg tcggatcggc aagggatttg aacacgcgaa gaatgtcggg catggcctgg
    23761 cctcttttta aagtccatta taatatgata atatcttcat ataatcaaga tttctttcgg
    23821 ccgctctccc ggcctgggcc gaggccgaca tcgatcattc cccggatccg ttgaaaggcc
    23881 ggggcgaatc cgccgtctgg tcacgcggca agatcagccc tccccttcga tacggcccat
    23941 gaaaaccgcc cggtcttttt ggccgtcgtc ttttaaaagc atcggcgttt cccttattcc
    24001 gcggcgcgct ttggattcgg cgaagaaata ttccggcgat ccacgggatt tgagacagga
    24061 tccgggacgt cgttagggat gagttgtccg ggagcgaaga agattttccg tggagggaga
    24121 cgaggccgca gtcttgaaca ggattcccag gcgtccgtcc gtcgcggcgg tttgtcgggc
    24181 tttctcgttg atccctatgc ctggcgtccg ggcgcagcgg aagaaagctc ttccgtccgg
    24241 gtcaaccggc cttgtgaatt catcgccgcc cgggcaacgg ccgcgacgtc cgcctggcgg
    24301 acgcgctctc gttttcggga tcttgagaag atcgcggata tgatccgaac ggctcctgca
    24361 aagtaaccgt gaattatccg cagcaggagc cgttctcgtc tttcggagaa tgcggctctt
    24421 tcccgctttc ctttttctcc tcggggattt cctcaaggac gaaaccgccg caacaagggc
    24481 tctttttcgg cttcgcgccg cccgttagac gcgaaaaaat ccctttcttc tcttttttgt
    24541 tttccatgga tccatgttct cctttgaaat cccttgaacg ttttcccgga tttaagccaa
    24601 caggttaaaa atcaagccgg acaggacggc cgaaacgaac acaatgatcg ataatccgat
    24661 gataatcttt ttcttgaaaa tgccggcgag catggtcagc tccggaatcg cgattcccgc
    24721 cccgctgatg atcaaggcga taacggcccc gatgctcgcc cccttctcca tcagggccag
    24781 cccgatcggg atcgcggact ccacccgcag gtaaagcggc atgcttatca aggcgacgat
    24841 gactacggaa agcggatggc cggctccggc gattttagcg aggagatctt cgggaagata
    24901 tccgtagatc gccgccccga tgccgacgcc gactatcatg tagatgaaaa ccgattggaa
    24961 gtctccccag gctttgaaaa ccgcggcgtg gagacgattt ttgaatcctt cgggaacgcc
    25021 tttgtccgga tcagggccga tttttttcac gcggacgttt tttatttgat tcgcccagcc
    25081 taatttctct agaaggaccc cgaaaacaat ggcaaacgcg gaggtcaaga gaaaaaatcc
    25141 cagggcgact ttccatccca gaaaagcgac cagcagggaa aaaatcagcg gatccatcaa
    25201 tggggaagag acgacaaacg acatcaccgc gccgaaggga acgccggctt ggaggaaacc
    25261 aagcgtcatg ggaaccgtgg aacaagcgca aaacggcgtg acggccccga aaagcactcc
    25321 catgacatta ccccaaagac ctttgccggc gatccagcga ttgattttat tcctgggaat
    25381 atacataaaa gccaaggcga tgatggcgct gatcccgaga aaaagaatcg agagctccgc
    25441 catgatcacc aggaaatatt tgatcgcctc gttcagcgaa ttcacggtcc cgtcctcgtt
    25501 tctaccgttt ccccggagcc gatttcggcg gggtgatttt tcgcaaggcg cgcgagatca
    25561 aagccatgtc acttgcgatt ttatcgattt tgccgcggcc gacggcctga agcgcccggc
    25621 cgaggcggcc gatttcgcga ccttccactc gggcgacgat gcgtgctccc cgcggggaaa
    25681 tcgtcacaca gcagacgcgg ccgtcatggg gggagatatt ccgccgaaga tatcttttct
    25741 tttcgagacg atcgacgatt cgggttgctc cgcttttcgt gagattcaag gcgtgcccga
    25801 ggtctttgat cataagtccg gggtgttccc gggccttttt tatcgcgacg aactcgatga
    25861 acgaaagatc cccgcagcat tctccgtcta ttcccccgct tccgaaaagc aatgtcatct
    25921 cttgcaaggc atcgaataag acggcgagcg cattcttcat ggcgacgttc ctttggaacc
    25981 agggatgagt ctcggggcag aatagccgaa atagttgatt aagtcaacta attgccgcga
    26041 cgcctcgaat ctctctttcg cctccccatg agagtccggg cgttattcga aaatcgggag
    26101 ggtcctagcc gtaaagcacc ttgcgcatgt ctttcaaacc gtgggcccgc tccaaccggg
    26161 cgatgatccc ggggtagtcc gcctcccggc cctcggcgac ggccagctcc cattccgaaa
    26221 gaagcggatc aacccggtat tcatgctttt tcgcgagcag cgcccggatg ccgcggagcg
    26281 actgccagtg ggtgatcatg atcagggcta gggcgtaaaa aacgatgttc gggccgggca
    26341 ggaaggcgat cagcccggtg aagggcgcca ggattccctc tccgaccaga agcagaatcc
    26401 gctgggtccg gcgcttatgc agaaagaagt ggaaccggag gtcggcgtgc ttctcctccg
    26461 cctcgccgca gagaacggtg accgcgggat ccttgaggcg gacggccttg gccagggctt
    26521 cctcctggcg aagggtccgg gcgggcagga gcatgagctt tttcttggcc gcttcccaaa
    26581 cctccttggt tttcgaaaag cgggccggca tcggccccgg cggttcggaa cagaacagcc
    26641 gcggatcttt ccgggcgtcc ttgacgaaaa aataagtgat catcttactc cttcgccgcg
    26701 gttcgccccg gaggaaattt ttccggatgg agaaggccgg cgagctcttc aagcgaatcg
    26761 aacagccgcg gaccgaaccg gctggccgcg ttttcgtcca ggcggaggat ccgtccgttg
    26821 cggacggccg tcgtggcccg caggcccggc cggctgttca gccaggcccg cgtccgttcg
    26881 aactcggcgg gggaaggcgc caggacgacg atgacgtccg gatcgtcccg gaccaggctt
    26941 tccacgctgt attcgagcca gcttttctcc aagccggccg tgacgctcgt tcctcccgcc
    27001 ttttcgacca aatcggtgaa atagctgtcc cgcccgcagg tccaaaggcc ctccccttgg
    27061 agcttgagga agacgcgcgg ccggacggcg actccggaaa gggcggcggc gacccgggcc
    27121 gctttgtccg tcagggcccg ggcgagaatc ccggcttcgg acgtccggcc gacgaccgtc
    27181 ccgatttttt cgacgagcgg gcggatttcc tcgagccgga agccgatgtc caggacgaaa
    27241 acccggaggc ccaggtcatc cagccgctcg attcgttccc agggattgcc gcggaaggcg
    27301 atgatgagat cgggagcggc tttccggatc cgttcgatgt cagggtcgag aaagcccccg
    27361 atcctgatct tttccgccgc ttccggagga taatcgcaat aacgggtgac gccggccact
    27421 tggcttccgg cccccagggt gaaaagaatt tcggtgatgt tcggggcgag cgaaatgacc
    27481 cggctcggcc ggccctccag gcggaattcc cggccccggt cgtcgcgaat caggcgctcc
    27541 tgggcgagga gcccggcggc gatcgaggcc gcggccagga tcgccggaag aagaaccaga
    27601 cgtcttgcgc tcaccggacc agtgtagtgt ccgcttgggg gatttgcaag atttccggga
    27661 gccgggctcg tttgtttttt tcgcgtccgg ttatttttcc tgggcgatca ggagcgtccg
    27721 gggatcgagc cggatcggaa ggacgccggc cggcggatta tcatgatcct tgaccacccg
    27781 gatttcgacg gcgtcgctct tggcctcgag ggtgatgaga acgtcgatcc ggccggcgtg
    27841 cggggcgagc aggaccggcg ttccgtccgg tccgaacatc tcccctccgg tctccttcag
    27901 ggacgcgctg aaccagatcg acacgttcat ccgtcgggcg aagtccttga accggtcgag
    27961 gtcgtccggc gcggccaggc cgaaattata tccgtcgacg atgatcgtat cggcgtcgaa
    28021 tttccccccg gtgatcatcg cctcgaggct gcgaaggact tgcccggttc tcatcccgtc
    28081 ctgcttgaaa ttcatgatga cccggttttt gatgatctcg tcgtaaacgg tttcggagtc
    28141 gcgcaggcgg tgggttttga cgatctcctt gaagatgtcc tcgtaccagg tcatgatgta
    28201 atccacccgg ctggagtagg agacgtggat gaccgccttc ccctggaaaa gcttgtcggt
    28261 ggcgatgtga acgaggcagg cggtcttgcc cacgcccttc ggagaggcca gcacggccag
    28321 gccgccgggg cccggaccgc cgtgggtgga atcctccagg acccggaggg ggctgcgatt
    28381 gatcaggtgt ttggcatcca tcggcgatat tctccttacc cggtctcgcg ggctcttttt
    28441 tcggcgtgtt gcttttgcag ctcctcggcc acggccgcgg gaacccggcc gtacttgagg
    28501 aattccatcg tgaattcggc cttgccttgg gtcagggacc gcagctggtt cgaatagccg
    28561 aacatctcgc tcaagggaac ttcggcatcg acccgggaga agctcccgtc ctcgatcgag
    28621 ctgacgatca ccccccggcg ctggttgatc gagccgaaca cggcgccggc gaattcggaa
    28681 ggaccttcca cggagacttt catcaccggt tcgaggatca cgggcttggc cttggcgtag
    28741 gcctggcgga aggctccgag ggcggccgcc tggaaggcgt tgtccgagga atcgacctcg
    28801 tggtactggc cgtccgtcag gacgacccgg acgccggtga cggggaaccc gatcacgccg
    28861 cccttcttga ccgccgtctt gcagcccttg tcgcaggagg gaatgaattc ccgggggatg
    28921 cggccgccct tgacctgatt gacgaactcg tacgccccgt cggccagcgg ctcgagatag
    28981 ccgatgaccc ggccgtactg gcccgccccg ccggtctgtt tcttatgagt gtaatcgaat
    29041 tctgccttct tggtgatggc ttcccggtag gcgacctgcg gctttccagt ggcgacctcg
    29101 acgttgtatt cccgcttcat ccgctcgacg tagacgtcga gatggagctc gcccatgccc
    29161 tggatgatcg tttcgtgcga ctcgggatcg acgaacgtcc ggaatgtcgg gtcctccttg
    29221 ctgaaccggt tcagggcctt ggccagccgg tcgagcgccc ttttatccgg cgcggagacg
    29281 gccagggaga taaccggctc cgggacgaag atggaggtca aggtataggg gcaagaggga
    29341 tcggtgaagg tatcgcccga ggcgcaatca atgccgaaca gggccgcgat gtccccggcc
    29401 gtggcttccg ggatatcctc catgtggtcg gcgtgcatcc ggatgagccg gccgaccttg
    29461 attcgctggc cggaccgggc gttctgaagc tccgtccctt tttggatccg gccttgatag
    29521 atccggatat aggtcaactg gccgtacggc ccgtcctcga gcttgaaggc caggaggacg
    29581 ggcggggcgt ccgggtcgga ggacaggacg acctcggccg gagcatcggg gggggcggat
    29641 tggtcgacgg cgatgttttt gacctcggac ggatcgggaa gatagcggac gatgccgtcc
    29701 agcagcgctt ggatcccctt gtttttatag gccgagccca tgaaaaccgg ggtcagcttc
    29761 cgggcgatcg tgccgcgccg gacggccgcg tgaatgagct cctcggtgac ccggtcctcg
    29821 agaatggcct cggtcagctc gtcggagaac atcgaggcgg cgtcgagaag atcattccgg
    29881 cggcggtgcg cctcctcgcg gaggtcgggc gggatttccc ctcggcggat ttcgtcgcct
    29941 tcgaacagga cggcctgcat ggagacgagg tcgacgacgc cctggtgacg gtcctccagg
    30001 ccgatcggga tctgcatcag gacggcgttg tgcttgagct tttcctccag ctggtcccgg
    30061 attttttgcg gattggcgcc gacccggtcg cacttgttga tgaaggcgag ccgggggacg
    30121 tcgtaacggc ggagctggcg gtcgacggtg atcgtttggg actggacgcc gcccaccgag
    30181 caaaggacca agacggcccc gtccagcacc cgcagcgacc gttccacctc gatggtgaaa
    30241 tcgacgtgac ccggggtgtc gatgatgttg atccgatgtt cggcccagga gacgctggtc
    30301 gcggccgagg cgatggtgat gccccgttca cgctcgaggt ccatgctgtc catggtcgcg
    30361 cccactccgt ccttgccctt gacctcgtga acccggtgga tcttgccgca gtaataaagg
    30421 atcctttcag acaacgtggt cttgccgctg tcgatgtggg cgctgatgcc gatattgcgc
    30481 attcgttcaa ttgtcgtggc catggccgtt acccttcgtg gaaatgcgag tttgccgggg
    30541 atttcgatgg acttcggaga ggaaaacaag aggcggaaga agacgaaccc cggaatcgaa
    30601 cggcgattct acccgaaacg ggacgggatt gcaagcagat cgaccggaag cgggcgcggc
    30661 tctatgatct ttatcgaaag aagaaaaagc ggccgatccg cgttaatccc tataaaaagc
    30721 aggtcctttc tatggttgcc tttttggtga gtgaacaatc ccaccttgga ttacctgttt
    30781 agatgagttg acatgggcct cggggggcgt tttttcttga cttcgcctcc gcgaccccat
    30841 tacgataagg cttgccggaa cgatggaaag gagagagagc catgggatta ttcggcaaat
    30901 cgtttgctca aaaagtcgat gaggccgtgg ccgcggtcaa ggccaaggtc cccgaggtga
    30961 aaaacctccg ggcggtcctg gacggcaagg tcgtgaccct ggaaggggat gctcccgatc
    31021 ttaacgtcaa aggccgggtc gtcaacgagt tcaatgccct ggtcaacacg gagaacacga
    31081 tcaacaagat caggctcgcg gagacgcccg ccccggcgac cgctcctccg gcggccgccc
    31141 cggccgaagc cgaacaggtg catgtcgtcg tcgccggaga caccctgagc gctctggcct
    31201 tgaagtatta cggcaaggcc gggatgtaca tgaaaatttt cgaagcgaac cgggacatcc
    31261 tggccaatcc cgatcttatc cgggtcgggc agaagctgcg gatcccgaag tagccttccc
    31321 tctgtcccgt cgtcggcgaa gaatacgccg gcgaaaatcg gaccggtcag cgccgtccgc
    31381 cgaaaagccc gccaaggagt tttcccagcc ctccgcccga ggagggggag gctgaacctt
    31441 ggaacaacat cccggcgaca tcgtcgagga tgctgccgtc cccgtctcgg tcgagcaggg
    31501 cggcgattcc gcccaatcca tccgccgagg tgtttttctt ccggctgaga gcgccgagga
    31561 tcagcggggc cagggccggt atgattttcg cggcgacacc ggggtccagc ttgaaacgcc
    31621 gggcgatcat gtccgtggct tggagacccg aagagcctag aaggccgccc agctgagcat
    31681 cggcggcttg gccggcgcgg ttgccgatcg ccccggcgat gtcatccaga acggaatcgc
    31741 tgccgtattt gttcaggatg tggtcgatcc gggcggcgcc tccctgggtt tccatctgcc
    31801 gcttcagccc tcccaggatc atcggaacga ccgccggaat gatctgggcg gccgcggcct
    31861 ttttgaggcc cagacccgcc gacaggtcac ggctcgcttg gccgccgtag gcttgcatga
    31921 attcgtcgag aaaggccatg ctcatttctc ttttttggat ttgcgcttga tgtcttgacc
    31981 atgggctctc ttgtacgatt gaatatcgac gaagcgtccc ttggcgtccc tcaccgcgta
    32041 aagcttcttc cccgtcttgc tcctcagaac cgttcttttc atcatatcct ccttttttgg
    32101 aattggatcc aaactctctc tccttgtaat tgtgtccaag acgcgatgga ttgtcaataa
    32161 gattcagccg tccggaaacg ccctcccggg ccgcctgtca agcctttgaa acctttccca
    32221 agaggggatc gtcccgcctt tgaaaaatcc ggccgaagcc gaggatcgca gagagaacat
    32281 cctctgttgg gggcaacctg cggagcggct ggcgtctgta agagcgttga catttccccc
    32341 cggcccccct acactgaacg cggaattcca tccccgcgcg accgtttcgt cggccgacga
    32401 acgcgagaag gacgcaccga tcatgagcaa ggataacgcc gcccgagatt acgatttcca
    32461 ggccatcgag gcccgctggc agatggagtg gaacgccgcc ggcgctttcg ccgcggccgc
    32521 cgatcccggc cgcccgaagt tttacgccct cgagatgtat ccctatccgt cgggccggat
    32581 ccacatgggc cacgtccgca actattccat cggcgacgtg atcgcccggt ttaagatgat
    32641 gcgggggtat gatgttctcc atcccatcgg ctgggacgcc ctgggcatgc cggccgagaa
    32701 cgccgccatt aagtccggca tccatcccca aaagtggacg ctggataata tcgcccacat
    32761 gaaggcccag ctccagagga tggggttcgc ctacgactgg tcgcgggaag tcaacacgtg
    32821 tttgcccgaa tactacacct ggaaccagtg gatcttcctc aagatggttg agcgcggcct
    32881 ggcctatcgc cgggaaagct gggtcaattg gtgcccgtcc tgccggaccg tcctggccaa
    32941 cgagcaggcg gccggcggtg tttgctggcg ctgctcctcg gccgtcgagc agaaaaagat
    33001 ggaacaatgg ttcctcaaaa tcaccgccta cgccgacgag ctcctctccg gtcacgccca
    33061 gctgggcaag tggcccgaac acgtcctggc catgcagaaa aattggatcg gccgaagcga
    33121 gggcgcctat ctgaatttcg acgtgccgtc gcttgggaaa tcgatccggg ttttcaccac
    33181 ccgcctggac acgatctttg gggcgacgtt cctcgttctt tcgcccgagc atcccctggt
    33241 cggcgatctg gtccgggggc cccgggaggc cgagcttcgc gcctggcttg ccaagacgat
    33301 cgccgccgcc cgggccaagc gggagatcgg cgaggccgag aaggaaggcg tggacaccgg
    33361 cgtcaccgcc gtcaatccgt tctcgggcga acccgtcccg atttggctgg ccaattacgt
    33421 cctgatggag tacgggacgg gagcgatcat ggccgttccg gcccatgacg gccgcgattt
    33481 cgatttcgcc cggaaatacg gcctgccgat ccgcgaggtc atcgtccccg ccggctcccc
    33541 gcgggatccg gcggccgggt tgttcgagga ccggggcgtc cttgttcaga gcggccccta
    33601 cgacggattg acttcggacg aggcggcaaa aaaaatggcc ggttgggccg aaagccaggg
    33661 cgtcggccaa gcggccgtca cttaccggct ccgcgattgg ggcatctccc gccagcgcta
    33721 ctggggcacg cccattccga tgatccactg ctccgcctgc ggaatcgtcc ccgttcccta
    33781 cgaagacctt ccggtccgca tcccttacga ggctcggttc accggagagg cgggctcgcc
    33841 cctggagaag atcccctcct tcgtcaacgt cgcttgcccg aagtgcggcc ggccggcccg
    33901 gcgcgaaacc gacacgatgg acacgttcgt tgattcgtcc tggtattttt tccgctatgc
    33961 ttcccccgcc gaggatcgcc tcccgttcag gcgggaagcg gccgaccatt ggctgcccgt
    34021 cgatctctat atcggcggcg tcgagcacgc catcctccac ttgatctatt cccgcttctt
    34081 caccaagctg ctccgcgacc tcggattgac cgccttggat gagccgttcc cccactactt
    34141 ggcccaaggc atggtgacga tgggcggcgc ggccatgtcg aaatccaagg gcaacgtcgt
    34201 cgacccggac gaaatgctgg ggcattacgg ggccgacgcc ctccgcctgt tcgtcctgtt
    34261 cgccgcccct ccggaaaagg aattcgcctg gaacgaggaa ggcatcgaag ggtgttaccg
    34321 gttcgtgggg agggtttggc agctctaccg cgagatcacg gccctgtcgg ccggggagga
    34381 gccgccggca ggcgggaccg tcgatccggc cgcggccgcc cgtctccgga aaaaaatcca
    34441 tcagaccatc cgcaaggtgg gcgaggacat cgaaatccga tttcatctca acaccgccgt
    34501 ctcggccgtc atggagctgg ccaatgtcct gcggaaggaa aaagaggctc ttcggaattc
    34561 ggccgagggg agggctctcc tggccgaagg aatggaggcg ctcatcctgg ccttggctcc
    34621 gttcacgccc catgtctgcg aggaaatgtg gcgggggacg ggccatgaga cgctggtcct
    34681 ccgcgcgccc tggccgtcct ttgatccgat cctggcgatg gaggagaagg cgacgatcgt
    34741 cgtcgaaatc aacgggaagg tccgggacaa attcgaggcc gagcatgacg cggccgagga
    34801 ggccgtcaag gaagcggccc tggccctgcc ccggatccgg gagctgctgg aaggcaaaac
    34861 cgtccggaaa gtcgtcgtca tcaagggaaa gatcgtcaac atcgttgttt gacgagggcg
    34921 gctcagggga gccggccccc ccggcgggcg gcgggccgcc ggcttggccg gcccggagga
    34981 atggacggcg cttgaatcga gtataatgaa gaggatcgaa tctgaagaca acgagggaca
    35041 aatgaacccg gggaaagcaa ggatgtttcc ggcctccttc cgggccggtc tggcggtcgg
    35101 gctggcgttc gcctgcgcct cgtgcgggta catggttcgg gggtcgggcc ggttcctggc
    35161 cgaccggaac atctcctcgg tttatatccc catcttcaag aacctgacga cgcgttacga
    35221 ggtcgatgtc aagctgacgg aagccgtcat cagcgaattt atctcccggg gccgggtcgc
    35281 ggtcgtcccg ggctccgagt ccgccgacgg gatcctggag ggtgaaatcc agggattcat
    35341 cgtcgagccc atcgcctata cgggagccca gggatcggcc gaccgctatt caatcacgat
    35401 ccgcgcctcc gtggttctga aggacatcaa gaaaaacacg gtcctttttt ccaatccggc
    35461 gtttgtctat cgaggggaat acgaagtccc cgaggggatg gatttcgaat cccaggaaac
    35521 cgaggctttg gaccaaatcg ccaagctgtt cgcgaaaaac ctggtggttt tcatcgtcga
    35581 gggattctga gggatggccg gatccttctt gcccgttccg gaccggcccg aggacgtccg
    35641 cccggcctat tttttctgcg gcgaggactt gtatgaagcc cgcggcttcg tcgccgagct
    35701 ccgccgccgg ttggccggcc ccggcgaggc ggccgttgaa gtcgagacct tcatcctggc
    35761 cgatcgccgc tggaccgaag ttctcgacgc cgcccgaaac atgccttttt ttttctcgcc
    35821 ttggcgtctc ttgatcgtcg agggaaaggg cgcgggacag gaggacctct cggccgggga
    35881 agcggccgcc ttccgggatt acttcgcttc tcccgccccc caaacgacga tcatcgttct
    35941 tttcgagggg aagatccgga aaacccggcc cttggggaag tcgatcgcgg ccctgcccgg
    36001 tcccctcgcc cgcatcgagg agatcgctcc gcgcaaggac cgggagatca agtccttgac
    36061 ggcggaaaag ttcgaatccc tggggaaaca ggcgacgccg gaagccgtgg aaaggatttt
    36121 agacatcgcc ggcggcgaga tcgcccggat cgattccgaa gtggaaaagc tcgccgttta
    36181 cttggggccg cgaagaacgg tcgaagccga tgatgtggcc gccctctgct cggccagaag
    36241 cttcgacaac tgggagttga ccgcggccct ggaggccggc gatacggaca aggccttgat
    36301 tatcatcgaa agcctgttcg aaaacggcga ggcgccggag atgatcctgg cggtcttatc
    36361 cggatttttc cggaccgccc ttctggccaa agccgggctt cgggagggcc gggatcccaa
    36421 ggaggttttc cgggaagcca agccgtcgat cagcgaacgg ttcggcggct tctaccagag
    36481 gatgctcggg gaatacctcg ccctgctcgg acggatcaag gacgccgacc tgcttcgctg
    36541 gctggaggag ctggaggaca tggaccgccg cctcaaatcg acggccgtcg cgcccaagga
    36601 aatgatccag gcgttcgtcg tcggctatgg aagaaaggcg cccgggcgcc ggatcacttc
    36661 gccggggcgt cgctgatttt cgtgatctgc cggctcaggc gggatttata ccgggaaccc
    36721 gtgttcttgt ggatcgcccc ttttttgacg gtttggtcga tgatcttgtt cacggacggc
    36781 agggctttct gagcctcgct cttgtccccg gctttgatca aaacgcgaag cttcttgacc
    36841 tgggtccgca gggaagactt gttcttggtg ttgacggcgt tgcggcgaag gctcgcgcga
    36901 tgctggcgtt cagccgaggc gtgtttagcc atggttttct ccttggaagt cgaagtataa
    36961 taaaagaaag gcctttaaaa gtcaaattcc gcccctggag aatgccttga gagtcgtcct
    37021 gcagcgcgtc cgggaggctt cggtccaaac cggcggcgag gtcgtctccc ggatcggccc
    37081 tggcttgtgt cttttggtgg gagtcgagaa aggcgacgcc gaggccgacg ccgaccgact
    37141 cgcccgcaaa gccgcttccc tccgtgtctt ctccgacgcc gaaggccgga tgaacctcga
    37201 cgtcgccgaa gccggcggag cgatcctggc cgtttcccaa ttcaccctgg cggcttcggt
    37261 ccgcaagggc cgtcgtccga gcttcgacgg cgccgaggaa cccgcccggg ccgcggccct
    37321 cttcgatcgc tttgtcgcgg cccttcgttc gaccggattg gaggtttcga ccggggtgtt
    37381 tcaggccgag atgctggtcc gcttggtcaa cgatggcccg gtcaccttcg tcttggattc
    37441 ggccggcggc cccgcgccgg ccggaaaacc gctcagctga acttgaaatc gcagatccgg
    37501 tcgagcattt cttcggccgc ggcccggaca tagcggcttt tatcccgggt caaacggacc
    37561 agcaggagac gggccgccgg gccgccgatc gccccaaggg cgagggccgc ggcccgccgg
    37621 acgtcgacgt tgggatcgtc gatcaagcgc ttaatgtgga cggcggcggc gcggtctttc
    37681 ttttcggcca ggacgcggag acaggcgctc ttggcgtacc agggaggccg gtcgagacgc
    37741 tcgtaaaggg cggcggccgg ccagttgtcg cgcctggaca gctcgcggac ggccaagtcc
    37801 cggatttcct cgatgctgtc ccccaggagc tcaagaaaaa cggaaggaat ccagggggcg
    37861 tccaggtggg ccagggcccg catcatcctc atcttcttgg cgcggtcacg gaaaatatcc
    37921 agcttcttga ggagagcgtt ggtgaaagct tgggtgtcgc cggtctgtgt ttccgccggc
    37981 aagaggcttt cgatatcccg gagggcctgt tcgaccacgt ccggagaggc gggttcgggc
    38041 cggcggagga acttggtcac ggtcagacca tttctaaaat atgtcccatt ttatcctttt
    38101 tggttttcag gtagtccgcg ttgatcgcgt tgggggcgat ctccaagggg acgcgctcga
    38161 cgatctccag gccgtagccg ctgaggccga tgaacttgcg cggattgttg gtgatcagcc
    38221 ggagccgctt gatcccgagg gcgaccagaa tctgggcgcc gatgccgtag tcccgctggt
    38281 cgggcttgaa gccgagccgg gtgttggctt cgactgtgtc ggctccctgg tcttggatcg
    38341 cgtaggcctg gatttttgcg gccaggccga tgccccgccc ctcatggttg aggatgtaga
    38401 ggacgacgcc cgatccttcc ttttcgatca tcgtcatggc cccgtggagc tgttccccgc
    38461 aatcgcaccg gctggagccg aacgcgtcgc cggtcaggca ctgggaatgg gcccggacca
    38521 gcacgggccg gtcgccggag atatcgccct tgacgagggc gacatgcgtt tccccgtgga
    38581 tctcgtcctc aaagatgaag atccggaagg ttccgtactt tgtcgggagg tcggcttcgg
    38641 agactttgcg gaccaggcat tccgtctgca tccggtactg gatgagatcg gcgatcgtca
    38701 ggatggggat tccgtgctcg cggctcaggc gttcgagctc cggccgccgg gccatcgttc
    38761 cgtcggggtt catgatctcg cagatcaccc cggccggggt gagtccggcc atccgggcga
    38821 ggtcgacgga cgcctcggtc tggccggccc gggccagcac gccgccctcc ttggcctgga
    38881 gggggaggat gtgtcccggc ttggccagat ccgacggctt ggtcttggga tcgatggccg
    38941 cccggatggt cgccgcccgg tcgaaggcgg aaatgcccgt cgtgataccc tcaacggcgt
    39001 cgatcgtcac ggtgaaggcg gtctggaagc gggccgtgtt ctcctggacc atgagcggaa
    39061 gctggagctg ctggagacgc tcccgggtga gggggaggca gatgagtccc cggccgtagg
    39121 tggccatgaa attgacgact tccggagtcg ttttttctgc ggcgaccatg aaatcgccct
    39181 cgttttcccg gtcctcgtcg tcggtgatga tgatcaggcg gccggcgcga acggcctcga
    39241 gggcccgttc aacgttcacc gcgtggggag ctttaacggc catggatacg tcctaacgct
    39301 tatggaagtg aagaaaatta tacacgtatt ttccgagcat gtcacattcg acgtgaacgg
    39361 ccttcccggc ccgcaggtcc gccaaattcg tccggcccag agtttccggg atcagctcga
    39421 cgtcgaacga tcccggcccg accgcggcca cagtcaggct gacgccgttg atcgcgatcg
    39481 atccttgggc gacgacgaac ggccggaagg cggccggcag gatgacggcc aggcggcggc
    39541 ccgacgaccg ggaagaaacg cgcttgatct tgctcgtgaa atccacgtgt cccgagacga
    39601 gatgtccccc cagcggatcg gccaaggtca ggggatattc gagattgagc gtctcgccgg
    39661 ggcggcggag ggaaaaattc gtccgctcga gcgtttcccg gaccgtgttg aaaaaaaggc
    39721 gatgtccttc cttgcggacg agagtcaggc agacgccgtc gacggccagg ctttgtcccg
    39781 gcgcgatcct cccggccgct tccggggatt cgacgatcat ttcccgcccg ttttcccgga
    39841 agccccggaa aatccccctg gcctggatga tgccggtgaa catcagatat acccctccaa
    39901 gatgaggtct ccgccgaggc ggaatgagga aacgcgggac aggcggggag cggcggccag
    39961 ccggtccgcc ccccggccgg cgaagagcgc cacggcgtcc gctccgccga tcagtatcgg
    40021 ggcgacggcc agaacgactt tatcgaccaa gccctcgtca aggaacccgg ccgccgtgcg
    40081 gcccccgcct tcgacaagaa gggcggcgat ttccctcgat ccgaggtcgg aaagaacacg
    40141 ccgcagatcc agccgcggtc cttcgccggg aagctcggcc acctcggctc cgcgtcgaag
    40201 aaggcgggcc ttgcgggcct cccaatcccg gtttccggcc cgggcataca acagcaccgg
    40261 cccgcccgac ggatcctcaa acagccgggc ttccggcgga gtccggaggc cgggatcgag
    40321 gacgacccgc aggacgggct tgccccttcg tcccggcggc cggacggtca ggcgcggatc
    40381 gtcgtgccga accgtttggg ctccgaccag aaccgcgtcg ttttcggacc ggaggaaatg
    40441 ggcgtagcgc cgggcttcga gagacgagat ccaggcggat tcccccgtcc gggtggccag
    40501 cttgccgtcg aggctgaggg cggatttcaa ggtgacgaag ggccgccggt cgacgatgta
    40561 cctggcgtgg gcttcattga gcccggattt ccgctccggg cggccggcga cgtccacggc
    40621 gaggcccgcc gtccgcagga accgtacgcc ccggccgcgg atgacgggat tgggatcgag
    40681 accagcgatg acggcccgga cgattccgga cgtcaggacg gattcggcgc aggggggcgt
    40741 ccgtccccag tgaacgcacg gctccagggt gagataaaga gtcgctccgc gtgcgttttt
    40801 cccggcccgc cggagagcgg cggtttcggc gtgctcccgc ccggccgctt cgtggaagcc
    40861 ggtcccgacg atcctcccgc ggcggacgac gaccgctccg acatggggat tcggggacgt
    40921 gcggccgagc gcctggacgg ccagggcctc ggccatgtcc aggtaggcga gatcgcgctc
    40981 gtcgatcatg acatcagggc ctcgacgaag gcggccgggt cgaagtcctc catatcctcg
    41041 gctccttcgc cagtgccgac caggcggatg ggcaggttgt actcatcggc gatcgccagc
    41101 accgcgcctc cccgggcggt gccgtccagc ttggttaaaa ctaatccgct gaggccggcg
    41161 cacttgcgga attcccgggc ttgaacgagg gcgttttgtc cgagagccgc gtccagaatg
    41221 agccaggtct cgaccggccg gccgtcgatc tcgcgggaga cgatcctctt gattttatcc
    41281 agctcgctca tcaagttggc gttggtgtgg atgcgtccgg ccgtgtcgac gatcatcagg
    41341 tcatatccgc cggcgaggaa ttcgcgggcg gcgtcgaaaa ccacggcggc cggatccgat
    41401 cccggccggc cttgatggac ggggacgccc agccggcgtc cccagatcgc caattgttcc
    41461 tgggcggccg cccggaatgt gtcggccgcc accaggagaa cccgtttccc ctcggccgcg
    41521 gcccgctggg cgatcttggc cgccgtcgtc gttttcccgc ctccgttgac tccgaccagg
    41581 aggatgacgc cggacaatcc cggtccttcg tcccggattt ggcggcggga aagaagctcg
    41641 atgagctcgc ttttaaggac ggattccagc gtttccgcgt cgtcgttttt tttggtccgg
    41701 ttccggacgg cggccgtgat tttttcggcc acgggaacgc ccgcgtcggc caggatcaag
    41761 gattccgaaa gagtctcgag aatctcctcg cgcggccgtc cggggccgaa gaccttcccg
    41821 aaacggtctt tccaggcctg tcgggttttc gccaggcctt cccagagtcg tcctaacatg
    41881 atgaaaggat tatagattcc gccgggggtc aagtcaacga atggaggaga cgcgtgcttt
    41941 gaggcggccc gccgatgtgt taaagtgaaa gatgaaatga gcgaaacggc ctggagcatc
    42001 gccccgccct cgaccgcggc tcccgccttg gccgccgccc tcggactgcc gccggtcctc
    42061 gggcagattc tggccaatcg cggtttcacg gacgccgccg gggcaaggct tttcctcgag
    42121 gccggcttgg aggacatccc cgacccgttc ctgctttccg gcatggacgc ggccgtcgcc
    42181 cggatcgaaa aagcgatccg ggaaaaggag acaatcctga tcttcgggga ttacgacgtc
    42241 gacggcgtgc tggccacggt gatgctccat aaggcgctgt cggcgctcgg ggggaaggtc
    42301 gattgcttca tcccggaacg gctcaaggac gggtacggga tcaaggacgg ccacgcggag
    42361 gcggttgtcg cgcgcggagc ccggctcgtc gtcagcgtcg attgcggcat caaatccgcc
    42421 ggcttcgtcc gcgcggccgc ggccgccggt gtggatgtca tcatcaccga tcatcacctc
    42481 ccgggacccg agcggccggc ggccctggcc gttctcgacc cggtcctgcc ggaagcgggt
    42541 tatcccgatc ggaacctggc cggcgtcggg gtggcgttca agctcattca agcgctctac
    42601 cagagagccg gacgggccgg cgttcttccc cattatctca agctcgtcgc cattgggacc
    42661 gtcgccgacg tcgtcgacct tcgcggcgag aaccgcatcc tcgttcggcg gggcctggcc
    42721 gagctggccc gggtgtccaa ccccggcctg cggagcctga tggaaatctg cggcctgacc
    42781 ggccggccgg tgaccgtcgg ggacgtcggc ttccgcctgg gtccgcggat caacgcggcc
    42841 ggacggatgg gccggacgga gctcgccctc cgtctctttt tctcgaagtc cgcggccgag
    42901 gcggccgccg tggccggcga gttggacgtc ctcaaccggc ggcgccagga aaccgaggat
    42961 tccatcctgg ccgacgccga ggggcgcgtc cgggccgggg gattggccga aacgcataag
    43021 atcttgatcc tcggggatga aaaatggcat cggggaatca tcgggatcgt cgcctcccgc
    43081 ttgaaggaaa tctttcatcg gccggtcgtc ctgttttcct acgacgacgg ctgcgccttc
    43141 ggctcggggc gatcgattcc cggattctcc ctgatcgatt gcctcgagga ggcccggccc
    43201 ttcttcctga gctatggcgg acaccggcag gccgtcggct gcacccttcg ccgggaatcc
    43261 gtgccggcct tcaaggaggc gatgaaccgg gccgtcgacg cccgcttgac cttcgatgat
    43321 ctgcgaccct cgatcgccat cgacagccgg ctggagttca ccgacatcgg ggcggacttg
    43381 tggcgcggcc tcgctcttct cgagccccac ggcgtcggca atccccggcc tgtttttctg
    43441 accgaggggg ccgtcgtcgc ggccgagccc cgcaagctca aggaccgcca cgttaagttc
    43501 cttctgcgga aggacggccg gacgttcgag gcgatcggct ggaaccgggg cgcctgggcc
    43561 ggggaatttc cggccggaag ccggatcgac ggcgtgttca ccttccagac gtccgcctat
    43621 ctcggcgaag agcgcccgta tctggcgctc gaggatctcc gggcgggatg agagaatcca
    43681 tggctcatcc gtcttcccga agccgggcga aaacggccgt ccgcgttctc ctcatcggct
    43741 ttctggccgt cctcctcgcc gttgttttcg tctttttaag ccgccggtcc gaccgtccgg
    43801 cggccattcg gcccgagacg cggccgctga ccacgcaaaa agtggacgtc aaggaaaata
    43861 tccatttcgt gaaagacacg ggaggaagaa acattctgga tgtccaggcc gcccggaatt
    43921 ttacggatgc cgtcggactc tatcacctca agggaccgga aggggaaaag ggcggcgggg
    43981 tccgcgtcga aagccgcgcc cgcgacggac aactgaagtt cgcggcgttc gcggaggaag
    44041 tgatctacga tgcggattgg acccgccttg tgttccgggg cggcgtggaa atccgcctcg
    44101 acgacctcac cgtgaaaggc gcctccttca cctatcacaa aaaccgggac gcggtggtca
    44161 ccaacgttcc cgcggttttc gagggccggc ggttccgggg cgactgccgc cgggccacgt
    44221 acaacgtcac ggatgaatat tttgtttttt ccgggggaat caccctggtc accgcgccct
    44281 gggacgacga ccccgttccg gtgatcatca ccgggaaatc gatgacctat gcccggcagc
    44341 cgcggacggg ggaaatccgc ggcgacgtcc gtatcgccca cggccggagc cgcggccgct
    44401 gcaacgacgt cttcttcgcc cagtttcccg acaagcccga gtttcggcta ttcgacttcc
    44461 acggaaacgt gaaatgggag gcggacgaac gggcggtccc ggcggccgtc ccggccaagc
    44521 cgaaagcggg cgaggcctct cccgcggatg acgaactgat cttcttccaa ggcgggcggc
    44581 agcggatgga ggccgggtcg atgatgttcg ttccttacgg aaccgagggc tgggcgcgct
    44641 acggctccat gcagggcggc gcccgggtcg acatcctcgg cgaagccggg cgccagacga
    44701 ctttggaagc gcgggagacg gtgttttttt atgatgaggg ttgggctctg caggcgttcc
    44761 ggctgcagac ccgggcccgg atccggggtg aggcggaagg ccgaatgcgg ctcctggagg
    44821 cgccgaccat caattacggc gccgattccg gtctcctggc ggcgaacggg acggcggccg
    44881 agccggcccg gacgatttcg gggggacggg agatcaccgc cgaatggatc gccctcaatc
    44941 tccaaacgaa aaatctcgat gcgcggggcg gagtcaaaat cgtttcccat ccggaagccg
    45001 gaaacgccgg cgacgccgcc ctgttcgcct ccggacggac gacgttcatg accgccggac
    45061 tggcccatta cgaaggcggc gagaggcaat tccgcctggg cgagggggcc cgcctttggc
    45121 agggccggga atccctggaa ggccgggacg tcctgatctt cgaggccagg ggcgacatct
    45181 ccgcgtccgg gaacgtccgg tcggtgtttt ttcatcgcga tcaaaagaaa aacgaggatc
    45241 aacgcgtgga agtcaccggc ggacgcatgg cccgtcagtc gaaaaaagga aacatcctct
    45301 tcctggactc ctgttccttg acggtcggag aaatcgttct gaaagccggc cggctgattc
    45361 tcgagcccgg cggcggagag aacggggaaa aataccgccg gatcatcgct gatcaggagc
    45421 gggtcaccgt cacccagggg gcgaacacgg cccggggcga ccgggccgtc tacgacctca
    45481 gcgaggatgt gatcgtcctg acgggaaata ccgtcctgga ggacctggcc aagggggtca
    45541 tccacaagga cggcaaattg actttccatc cctccgatgg taggatttta atcgaaaaca
    45601 aggacgccga gcgatccacc accatcatca agtcgtgagc gatgaaatcg tcgctgaatg
    45661 cttctcattt atccaaatcc tacgacggca aaaaggtcgt cgacgatatc tccattgaga
    45721 tccaaagagg agagatcgtc ggtctcctgg ggcccaacgg ggcggggaaa acgacgacgt
    45781 tttccatgat tctcgggctt gtcgcccgtg acgcgggtcg gatttatctc aacggcgagg
    45841 acatcacgga cgatcctatg tatttacggg ctcggaaggg gatttgcctt cttccccagg
    45901 atccctcctc tttccgccgg ctgacggccg aggataacct tctggcaatc gcccggccgg
    45961 cgggccggcg gcgcggagac gcccggagga agctccggga atacgggctg gaatccctcg
    46021 cccgggccaa ggcctatacg ctgtccggcg gcgagcgccg gaggctggaa atcgcccggg
    46081 cgatgatgac ctctccggaa ttcatcttgc tggacgagcc gttcaccggc atcgatccga
    46141 tcgccatcca ggatttgcag cggatcgtcc ggaacatgaa ggacgacggg ttgggcgttt
    46201 tgatcaccga tcacaacgtc caggacaccc tccggatcgt cgaccgggcg gcgatcatcg
    46261 acaagggacg gatcgtcgag gaaggttctc cggaagcggt cgtcgcctcc ccggaagtgc
    46321 ggcggcgcta tctcggcgag gattttcgag tttaaacgga gcgcaacggc ggatcaccat
    46381 ggctttaaaa cagcgtcttg atcagcggca ggtccagaag ctgattctgg ctcccgccct
    46441 gcagcaggcg atcaagcttt tgcctctcac caacctcgag ctgatcgagg tcatcgacga
    46501 ggagctgtcg gccaatcctc ttcttgagct cgaggaggaa agtccggaag tgaagagcgt
    46561 cgaggaaacc ccggcggccc caagcgcgtc ggaagatctt cccgcgggca cggccgggga
    46621 agccgaagaa tccttggccg aggaccggga attcgaatcc tatttccagg aatacatcga
    46681 cgacggcttc cgggcggggc cggccgagcc gccggaagcg cccaacatcg aaaacatgat
    46741 ctcccagagc gcctcgctcg gggaccatct gacctggcag gccaatttga cgttcttcga
    46801 tcctctcgac cgggagatcg ccgagcggat catcgggaat atcaacgacg acggatacct
    46861 gacgtcatcc ctggatgaga tcgggctcag cgccggggcc ccgatcgagc gcgtccgcga
    46921 tgtccaggct aagatccttc agttcgatcc ggtgggctgc ggcggcctgg atttgaagga
    46981 atgcctgctg gcccagatgg acaacctgca aatcaaggaa gaagccgccc gggcgatgat
    47041 ctccgagcat cttccccttc ttgaaaagtc ggatttcggc cagatcgcca agcttctcaa
    47101 ccttcccctg gccgaggtca aggttcatct ggagatcatt aagcagctgg atccggcccc
    47161 gggccgcaag tacggcgagg accggacgca tttcgtcgtt ccggacatca cggtctccaa
    47221 ggagagcggc gagttcaagg tcgccctcaa cgatgagggc ctgccccggc tccgcatcaa
    47281 cgcctattac cgccggcttc tggcccgggc cttgaaggag aatccggagg cctaccggta
    47341 tctcaaggac aagatgaaga aagctctctg gtttttgcgg agcctggatc agcgcgacca
    47401 gacgatttac aaggtcgcca aggcgatcgt cgaccgccag aaggatttct tcgaaaaggg
    47461 gctcgacggc cttcagccgc tgacgctcat ggaaatcgcc caggagatcg gggttcacga
    47521 gtccacggtc ggacgcgtcg tcgccaacaa gtacatgatc actccgcgcg gcacgtattc
    47581 cctgaagtat tttttccata agtcgctcca gggcggattc ggcgaggaga tctcctctct
    47641 ccgggtcaag gaccgcctcc ggcgcctgat cgaatccgaa gacaaatccc gcccgctgag
    47701 cgacatggaa atcgaggaaa tcctgacccg ggagaatttc cccatcgccc gccggacggt
    47761 ggccaaatac cgccagcagc tggatatcct tccctcgcat atccgcaagc gcaaattcct
    47821 aatggaggag tcttgatgaa cgtccacttc acggctcgac acgcggcgat ccctcccgac
    47881 ctcaagacct atgccgagaa gcgcctggcc ggcctggccc gttttctgga ccgggcgacc
    47941 gaggcggatg tcgtcctctc ggctgagaaa agccggcagc gggtcgagat ccaagtccgc
    48001 gggaagcagg gccggatccg cgtcgcggcc gacggccacg acgcggccgc ggtgatgaac
    48061 gaagccttcg accttctcga cgtcaagctg aaaaaggagc gggcgaaatt ccgggaaaaa
    48121 aaacgccggg gcgggcgcga aaccgccgcc gccgcggccg gggaaaccgc cgccgggctt
    48181 ccgccgcgtg tcgtccgggc cgattatttc tccgggaaac cgatgtccgt cggcgatgcc
    48241 gtcgtcgaat tcaacctccg gaaaaaagag gttctgatgt tccgcatgga gggagaggaa
    48301 gaccgctggg cggttctctt ccgccggaaa aacggaacct tcggcctggt ccgtccggaa
    48361 tgaacgccgt ccggtccgcc gcgccggccg tcgtctcggg agggctcctc cggatgttcg
    48421 gcctgggcgt gctgatcatc ggcgacagcg gagtgggaaa aagcgagagc gcgatcgaac
    48481 tcatcgcccg gggccaccgg ttcattacgg acgatgtcgt tgaagtccgc cgccgggccg
    48541 acgggcggat catcggccgg gccccgcggc tgagccgtca tttcatggag ctgcgcggcc
    48601 tggggctcat caacgtccgg tatattttcg gcccgaaatc gattctccgc gaatcgacga
    48661 tcgacctggt catccggttg aagaagtgga gccggaaaaa ggaatacgac cggctgggcc
    48721 tgcgctttcc cgaagaccac ctcattcttg gaacccgcgt cccccagctc aatatccccg
    48781 tcgcccccgg gcggaacatc gccaccctga tcgaggtggc ctgcgtctcc catcttttgc
    48841 tccggcgcgg ccgccgcgcc ccggatcaga tgctccggcg tctgaaccgg gcgttcgccg
    48901 cgggaaggac cggcaagcca tgacccatcg acccagccgt ttcctgatca tcacgggcct
    48961 ctccggatcg ggaaagacgg tcgccagccg gtttctcgag gacctgggct ttttctgtgt
    49021 cgacaatttg ccggccaagc tcatcccctc gtttgtcgac ctccggcggc ggaagtcgga
    49081 ggttctggaa cgcgtcgccc tggtcatgga tatacgcgag cccgggttcc tgactgattt
    49141 tcccaaagaa tgggcgaaaa tcaagaaaat tcagggagcc cggcttctct ttttcgacgc
    49201 caccgacgga gccatcgttc agcgtttcag cgaaagccgg cgtccgcatc cgttggcccg
    49261 cggtcgttcg gtcctggacg ccgtccggct ggagcgcaag cggctcgcct ccatcatcga
    49321 gctggccgac gacgtcatca acacgtcgtc gatgaccagc gcccagcttc gcgacgttct
    49381 ctcccggcgt tttttccggg ccgacaagca gccgttcatc atccgcatca tcagcttcgg
    49441 ctataagcac ggcattcccc tggatgccga cctggtcttc gacgcccggt ttctccccaa
    49501 tccgttctat gaggacgatc tccgggagaa gccggggacg aacgcccggg tccgtgaatt
    49561 cgtcctccgg cggcccgaaa ccaaggattt cctggccgag ctgggccggt tcctgaattt
    49621 tctcctccct cgtttcgagg ccgagggcaa gagccagctc gtcatcggcg tcggctgcac
    49681 cggcggatac caccgttccg tcgtcatcgc cgccgccctc cgggatgtcc ttcgcggccg
    49741 gaaatatgat ataaaggttt ttcaccggga tattgccaaa tgatcggcgg cctcatcgtc
    49801 atgcacggaa agttggggga ggagctcctc aacgtgctga cgatcatcct cggggaaacg
    49861 gccaacatcg aggcgatttc catcggctgg tacgatgatg tcgaggaatc gaagaagaaa
    49921 atcgcctcca gcctaaagcg ggtcgatcag aaaaacggcg tggtcatttt caccgacatg
    49981 ttcggcggca ctccctccaa tctcagcttc accttccaga aggacggaag cgtcgaaatc
    50041 atcacggggg ccaacctgcc gatgctgatc aagttcattt ccctccagcg tggaatgtcc
    50101 ctgcgcgacg tggcccggaa ggtcgtcgag cagggccgga agaacatcca cctggctagc
    50161 gccatcctgg acgggaaaaa atcctgagat gatcgagcat gtcgtccgca tcaagaaccg
    50221 cctcggactt cacgcccggg cggcggtcaa gttcgtcaac ctggccaacc ggttcacggc
    50281 cgacgtccgg atcgagaagg acggaagcga cgtggacggc aagagcatcc tcggcatttt
    50341 gaccctggcc gcgacccagg ggacggaaat caccctgcgc ctcaacgggg acgacgaggc
    50401 ggcggccttg aaggccatcg tcgatctgat cgacggccgg ttcggcgaga aagagtagcc
    50461 ggagaaccgc gtatggatta cttgcgcctg cggggaatcg gcgtctcccc cgggatcgtc
    50521 atggggccgg tggtcctggc cgaccggacc ctgttttcca gccggaaggt gtatatcccc
    50581 ccgtatcgcg tcgccgatga gatcgagcgc ttgaacgagg ccctccggcg gacgggcgat
    50641 gaaatcacga cgatcaagga ccaggtccgg gcgatggccg gcgacgaaca tgcgttcatt
    50701 ttcgaagccc acctgctgat tctccaggat ccggcgctca cctccggatt ggaacgcctg
    50761 atccgggagg aaaacatccg gacggaatcg gccctgagcg aggttcatga gtattaccgt
    50821 cgtgtctttg acgcgatcgc cgacgaatac atccggcagc ggaaggcgga cgtgaccgac
    50881 gtcctgaccc gcatttaccg caacttggac gccggcgaaa aattgtccgt ctccgacgac
    50941 ctgcggggga aaatcctcgt cgcccacgac ctcctgccct cggaagccgc catccgcctc
    51001 agccagggcg gcgtcctggc cgtggtcctg gacatgggcg gccccacctc ccatgccgcg
    51061 atcctggccc gttccctgga catcccgacg gtcgtcggcc tccgcgacat cacccagcaa
    51121 gtcagcgccg gcgacgagct gatcgtcgac ggcacggacg gcgaggtcat cgtcaatccg
    51181 ccgctgatga tccggaggga gttccagagc aagaaggcca agtatgaagc ctaccgcaag
    51241 gaactccgcc ggattgcgac ctggaccccg gtcacccttg acggaatccg gttcgtcccg
    51301 atggccaaca tcgagctgcc cgaggaagtg ggtcacgccc tgtcctacgg ggccgagggc
    51361 gtggggctgt tccgctccga gttcatctac ctccaacgcg cgaccctgcc caccgaggag
    51421 gagcatttcg ccatctacga ccggctggcg cgggacgctt ttccccgccc ggtttatatc
    51481 cggacgattg acatcggcgg ggagaagact cttccccagc tgaaaatcga ggccgagccg
    51541 aatccggctt tggggctgcg ggcgatccgg ttctcgctcc aaaaccgcga attgttccgg
    51601 gttcagatcc gagccatcct ccgggcttcc gtccggaaaa atgtccgggt catcatcccg
    51661 atgatcacgg aaatggagga gatcggcgaa atccgcgttc tgttccgcga agcccgggag
    51721 gagctggccg cccagggtct cggcttcgat cccgacgtcc ccctcgggat catgatagaa
    51781 gtcccggccg cggcggccat cgccgatctg ctggcccggg aagtggactt cttcagcatc
    51841 gggaccaacg acctgctcca gtatttcctg gccgtggacc ggtccaacga atacgtttcc
    51901 tacctctaca agcctctcca ccccgcagcc ttgcgtcttt taaagcatgt catcgtctcg
    51961 gccgactgcc agggaaaaga cgtcgccctc tgcggagaga tggccgccga tcccctcgcc
    52021 gccgtcgtcc ttctcggtct cggtctgcgg acgttcagca tgaatccgat tttcatcccg
    52081 cggattaaga aagcgctgcg ggcggtggaa gtccgggttg tccggcgggt ggttcagaag
    52141 gcgatgaatc tgcgctcggc ccaggaagtc gaggaattca tcatcgaaaa gctcctcttt
    52201 aagtatcccg atgcgttctt gatgacgagc ccgtcatcct gacgggcggc ggcaggaaat
    52261 gggggcgtcg ccggcggctc ccccggagga ggagggacgt catggaattc agccgtcttt
    52321 tggtcgggct ggccgtttgc ctatgggctt tcgtgatcgg attttcgatc gtcgccttcg
    52381 gccagatcct cttggccgtc cgcgagatcg cgctcaacac gcggaaggag gagggcaagt
    52441 cccaccacgg gcattacgcc atcctccttg tcatggcgaa aatcaacact gtgttgggct
    52501 ggatcgtggt cgtcggaggc ctggcggccg ggatttatgt catcgtcttc ggcgttccgg
    52561 cgttcaccgg gctgacgacc ccggtcgtcg attccgccta tttgcgcccg taaacgtctt
    52621 cgtagcggac gatatcgttt tcgtcggaat cgcccagcca gatctccatg attcgggcgg
    52681 gccggggccc gacgccgcgg acgcggtgcc gggcttcccg cgggatgtag atttcctcgt
    52741 cggccgcggg ccgccacacc cggtcgccca ccgtcacctc gagtccctcg tcgagggcca
    52801 cccagaactc cgccctccgg cggtgggatt ggagggatag ggagcctccc gggttgaccg
    52861 tgatgatctt gatgcttccg gcctgttcat ggggaaacgc ccggaacttc ccccacgggc
    52921 ggtgatcctc gatgatccgg gagcggaaat tccgctcgtc ctcagaaggg gacatcgtcc
    52981 tcgccgaggg aagccggcgg ctcttcatcg gagggaaaat cgggcgaggg ggcgtccgga
    53041 cgcggctccc ggtagggcgt atcgatttca gtcccgcgcg gctgggcgtc gcgggggccg
    53101 aggaggacga tgttgaacgc ctcgatttcg gttgttttgc gcttgttgcc gtcgcggtcc
    53161 tgccaggacc gcgtccggat cttgccctcg accaggatct gtttgccgac ttccaggaat
    53221 cggtcgcaga attcggccag cttcgcccag gccacgatgg aatgccattc ggtccggatc
    53281 gacgcttggc tggtttgggg attgaacgag cgttcgttgg tcgccagggt gaaacgggcc
    53341 aacgcccggt cgctttgggg gaagtgccgc aattccggcc tctgcccgat ccgtccgatc
    53401 aggatgacct tgttcaagct gttgacgtct ctcatggggc ttcctgcagt tccttgattt
    53461 tttgctgagc ctgccgggct tcctcctcga ggggatattt tgaaagaagc agacggagga
    53521 cggcgagggc ctcggttttc tttttcattt cggccagggc gaatcccttc ttgagataag
    53581 ccgcggcgat cttgtcgctc atgggatacg tcaggatcaa gtcatcgaag gcctcgatgg
    53641 cggctggaaa ttttctctgg ctgaaccggc attcgccgat catgtagagg gcgttgtcgg
    53701 ccaacgggct ggcgccgaat tgttcgcgat agagggcgaa gccgtccacg gccaagtcga
    53761 ggttcccttt gctgaaatca gccatcgccg cctgataggc ttcctgggcg gagaggccgg
    53821 acggagggac ggtcgcggcc ggcggctccg ccgccggctt tcgggccgct ccggcttttt
    53881 cctcgggctt gggctttccc cccgcctccg gagcggccgt cgtctccccc gccggcgttt
    53941 tggcggactc gagatcggcg gccagttgaa gaaggcggtt ttcgatctgg gccagctgtt
    54001 cctggatgct ccgcacctgg ccggggattt cgctccatcc ctcttgattc cgggcttgga
    54061 aaatccggaa atccttgaat tgggcggcca agtcgcggac gatatcgccg agtcctttca
    54121 gatccgcgcc cgcttgatcg atcttcttct cgatccgctg agtttggatc ttgagcaact
    54181 gaatgtcttc atacatcagc tcataggcct tcttgctgcg gtcttgggcc gcggcggaga
    54241 gggaaaggcc cgctatcagc aggacggcgg gccacgaacg cttcattaag gttatttttc
    54301 gatgacgagg aactgggccc ggcggttgtt cgcccaggcg gtttcgtcgt gacccatatc
    54361 gagcggctgg ctcttgccgt aggaaatgat cctcatccgc tcggccggga tgccgagaga
    54421 caacaggtag tccatcgtgc tcttggcccg tttttcgccc agggccaagt tgtattcctc
    54481 ggtgccgcgc tcgtcgcagt ggccctcgat caggattttc gccgtttgga atttcttcat
    54541 ccaagcggcg ttggcttcga gaatcccctt cgcgtcatcg cggacaaaat acttgtcgta
    54601 atcgaaacgg atgttcttca gggcccgctc ctggttgatc tgatcgagcg tctttctcag
    54661 gaagagctct tcttccgaaa gcggggcggc ttcggccggc gccgtttccg tcacttgctc
    54721 cacgacgggc tgttccttga cctgcggagg aggcggagga gtgggttttg ttttcgcttt
    54781 gcaggaaact cccaagccta gggaaatggc aaggagacag acaagggcca aaacgattgt
    54841 ttttttcatt gagtcctcct ttgtcagtgt gatcgttgag ggcacgatga aatcgtcccc
    54901 cgccaatccc ctatatacat ggattaagac ggtgtgtcaa ttcggatcgt ctcaccggtt
    54961 gcgcgaataa taaaatggat taatttacgg ggaaataagg cggatcagcg ggtgaatccg
    55021 cttcttggga atggtccgcg aatgaggaag ggacggtttc ccctgtccgg gcgatttcac
    55081 cggtcttttc gggaggcgca aatcggcgtt ctttcgggga gagacggccg gagggagatc
    55141 ccgcttcatc gtgtaaagac gtctccaccc ctccgccgga gagccgggtt cccttcgggc
    55201 gttgcggacg tcctccggat ttaccgggcc gttttcaatc ggaaatggag ccgcgtgacg
    55261 tcccgtcccg ggcgcgggcc tcactccgcg gttttcactg tctccgcgga caagggggga
    55321 gagatcgttt cctttttccc ggccgggtct tccggctcct cgaaaatgaa ataccggtat
    55381 gttccgtcgt tttccttgcg gaggcataat cggtcgaagg gcgggatttg ggcggcggag
    55441 gcgtcccaga tcgcgtaacg gatcttgttt tccctaagga aacgccgtaa tttcctcagg
    55501 ccttgaaggc gtggaaaatc cgtcacgaaa acccggcgtc ggcccagcgg atcgcagacc
    55561 atcccgaaca tggccgtcat cggcggggag agaaccctct gttgattcaa aggtccgagg
    55621 agaacgggct cgaaatcgcc tttcattcgg cgatacgcgg cgccgcggcg ggtctcgtcg
    55681 atcgtcatga aggggcggct ccaagtcagc cggtcgaagc cgatcttccg ttcggcggga
    55741 aaagcagtct gtcccccata ccagtcgatt ctgctggctc cggccagcca catgattccc
    55801 gccgccgttt ccgcgagaaa aacggcggcc agaaggatcg cccaggaggc gatccatttc
    55861 cggccgcggc tttcccaaaa tttgtcgaga acgaggacga ggaagggcaa accgattcca
    55921 agcggccaca ccgtataccg agcccaccaa tgcattggcg tcaaaagaaa aaccagggcc
    55981 gcgcagagga ggagcgtgat ccgggctcgg cgcaagccgg gaggcggccc ggattttttc
    56041 ccggttaaac aggcgaggat gacggccggg aggcatccca aaagccagag aaggccgagc
    56101 cctcccaagc gaccgctggc gctgcagacc gaatacggcc agtgagccgg gccgccctgc
    56161 atccagacat aagccacctg cttccagccc ggccagttcc tgatcacggg gggagtgttg
    56221 gcggccttgt tcaagataaa atcaaccgat ttggcggatc gaccggggtc accgattttc
    56281 agcgtcaggg ggtaagccgg atttccggtg tgggcgaggt ttcgagcgta ccaatagccg
    56341 ccgaccaggg cggagatgag gaaaacaacg ggaatccagg cccaggtccg aagattcgcc
    56401 gcccgccggg attcggccgg caatctccgc cggcgaagtc cgagaacgat caccgcggcc
    56461 gctccggcca cacccgccgg aaggattccc gagctcttga ggccgaggac caggccggcg
    56521 ccggcgccga aaacggcgac ttcatcccaa gcggcgaatc gaccgcgcgt gccggcggcc
    56581 aggcgggaca aaacggcgaa gagagcgacc accgccgagg cgaaagccga atccacatat
    56641 gtcccgttca actgctggat atggaccggc gccagagcga agagcagacc ggcggtcagc
    56701 gaagcttccg ccgaggcccc caggcttgag gcgataaggg ccgtgccgct gattccgatc
    56761 gccagaaacc aggcgttgag gagatgaacc agcccggaga tccccgtcgc ctgaacgacg
    56821 acgaagccca tcaattcggc ggccttggga tagccgttga tgaagagatc ggtgaagaag
    56881 ggcttcaaag gttgcggggc gacccagtgg acaaagcctt tttcgctcca gtaatgaacg
    56941 gccggcagat ggtagatcag gcaatcgccg aagagataag gatggaaata agcgacgatg
    57001 acggtcaagc ccagaagggc gaggagaatc gggatgactc cgcgcaggat cggagccagg
    57061 ggagacgggg cggcggtctc gttcggcccc gtttcaggga atcggcggaa aagccggtcg
    57121 aaaccccagg ctccggcggc cgccgcggcc agaaccgcca gggcggcggc ctgcgaaatg
    57181 acgatatgag ccaggctcag ggcaatggtg agaacttgga cgagagctaa aaaaatcagg
    57241 ccgccgctca gccggcggaa ccaggtctcc ggcgatgatc gggaaagaac ggttgtccag
    57301 gccgtcgccc ccttgaatcc catggagatt ttcccttctt tacggccaat gtaccacgga
    57361 gagaacggga aatcgaggcc ttgggcttca tgatcaggac gaccggatca gtttgaccag
    57421 tcggcgagct tgttttcccc catggacgtc agcttgcgga aattgcggcc gtcgacgtcg
    57481 atcgcgtgga tttggttccg gccgctgacg ttggaggtga agaggatgtg gcggccgtcg
    57541 ggggaccagg agggagattc gttccggccg tagatttcgg tcagcttgga aatctgcccg
    57601 gaccggatgt tgtacaggta gagatcgaag gcgttgtcca cccgggcgac atagacgatc
    57661 ttgtctccgt tgggcgacca ggcgggggcg tcgtgatggt tggctccgaa ggagatccgg
    57721 cggacgttgc cgccctcggc gtccatgatg tagatctggg tcgttccgct gcggtccgag
    57781 gtgaagacga tttcccggcc gttgggggac caggaggggg cgatgtccac cgccggattg
    57841 aaggtcagct gcttgatgcg tccgaccctc gtggggttgg actcgatatc ggcgacatag
    57901 atttcgtggt ttccgttcat ggaggaggaa aaggccagct ttttcccgtc cggcgaccag
    57961 gccggggtgt aattctgccc tttgagggag acaggcgtgc gggcccgggc tttctcgaag
    58021 acgtcgagga tataaactcc gacaaccagg ttctgccagg agctgtaggc caagcgcttg
    58081 cctcccgggt cccaggcggg attggaatcg ttgacttggt tgtagatgag gcgggtctgg
    58141 ttggccccgt cgtaatccat gagatagatt tcctcgttgc ggtcccggtt gctgacaaaa
    58201 gcgattttcg aggtgaaaat cggcttttcg ccgtacgctt tcataatttc atcggccgtc
    58261 gcgtgggcca ggtagcggag atttttatcc ttgtactgaa aacgccgcgg ggcgatgatc
    58321 gtgcgcttcg atttgacgtc gtacagaacc caggaaaaga tgatccctcc ctccggctcg
    58381 gcttccaccc ggccggtgag aagaacgttg gcttggatgg attcccaatc cttgaactcg
    58441 ggactgttcg ggtcggtcag cggccggatg tatccgtaat acgacttcgg aagaagctcg
    58501 aagatccggg tgtagcggat gtcggcctcg aggacggagt gaatctcggc agccgcgtcc
    58561 ttggccgccg gggcggcgac gaacggcggg agagcgaagg ccacggccgg cattccttcg
    58621 gtgatccgca tgttgaattc ctgctgggcg gaggccccgc cggccgcgga cagaaaaagg
    58681 atgatcgtga caaggacgtt ttttttcatc gggcatgctc gaagaggaga ttgatcccca
    58741 agtattcccc gtcgtaatcg tcggggagag gggggaaatg ggcggcgttg gcgaccgccc
    58801 ggacggcgat caggtcgaag gcccggagac cgctggactg cttgatttcg atcgtggaga
    58861 tcgaaccgtt gcggtagatc cggaaataaa cgagggtttg gagaacgttg ttgggcccgg
    58921 ggtcgatgct gccgggaaac cagttgaccg tgatttcatc ccggacgcgg aggaggtaat
    58981 aggcgtaagg gaattcggcc aggccgtcgc cgaatccctc cccgaacccg ccgcccgaac
    59041 cggcgcctcc gggaccgatg gtcaggccgg tgccgccttc cccgcccccg cctgtcccgg
    59101 cggccgaccc ggacgctccg gccttccctc ccggacccgg cttggagatg gaggccctct
    59161 tttcggcggc ggctttgcgc ttcgtcttgt cgtcgacggg gtaccgaagc cgtgattcgg
    59221 tcttggggac gactttctcc ggaacggtca ggtcgcggag ggattccggc ttgctttggc
    59281 tgaccgccgt cgttcccccc gcggctttgt ttccgccgcc ggggagccgt ccgtcgccgc
    59341 ctcctcctcg tccgcccccg ccccctccca ccatccccat cgaaatgtaa tggatgacgc
    59401 ccttgggcga gggcttcttg ggaagggagg gattgagaat gatgaaggaa aacaggatga
    59461 cgtggccggc ggccgaaatg ataagggccc gcttgaaggc ccggtcgctg ccggcggcgc
    59521 tccaggtcat tttcggccgg ccttcttctc ctccggccgg gtcatgaggc cgaccacctc
    59581 gactcctgtt ttcttgacga tgtccaggac gtccatgacg aagccgaagg ggaggctctt
    59641 gtccgcccgg aggtagacga ctttcttgct tttcccgtag aaagcggcct gaagctggcg
    59701 ttcaaggagg ttgatgttga tgatcgagtc cccgatatag acggactggt cgggacggag
    59761 ggtgatggac aggccctcct cggccggggc cgtgtcggtt tccgccgtcg gaagatcgac
    59821 cccgatgccg ctttgcatca tcggggccgt gaccatgaag atgaccaaca ggacgagcat
    59881 cacgtcgacg aacggcgtga cgttgatctc ggaaagggcc gtgccgacgt cgcgcttggc
    59941 ccggacggtg ggaagcttag ggagaaccat agagccgttc cgcgatgctg agaagctcga
    60001 gggaaaaatc ctccatgtcg gtgatctggt ccttgagctg gtggaggaac atgttgtagg
    60061 ccatgaccgc cggaatggcc gcgaacaaac cgacggccgt ggcgatgagg gcctcggcga
    60121 tgccgggggc caccgagacc aggctggtcg atccggtgac gccgatcccg atgaaggtgt
    60181 ccatgatgcc ccagaccgtg ccgaacaggc cgatgaaggg cgagacgctt ccggtcg
//
LOCUS       DAOOXH010000006        57777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_445_length_57777_cov_8.620422, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000006 DAOOXH010000000
VERSION     DAOOXH010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 57777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57777
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_445_length_57777_cov_8.620422"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PKV64_01600"
     CDS             <1..466
                     /locus_tag="PKV64_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HPN15804.1"
                     /translation="AKARRLKLEGLLDIVFIDYIQLMRAGGRFENRNQEMSFISRSLK
                     ELAKELAIPVVGISQLSRAPEKARREPRPMLSDLRESGAIEQDADVVIFIYRPEMYFT
                     PEEKAVQTELRGIGEVIIAKQRNGPTGQFELAFQNKFAKFENLETSHRGPEE"
     gene            470..1249
                     /locus_tag="PKV64_01605"
     CDS             470..1249
                     /locus_tag="PKV64_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN15805.1"
                     /translation="MAAPPRSIIVRFFEQLGELSLLAGRTFRSFFQKPWEGKIFLQQL
                     EEIGLRTLPVVSLTAAFGGLVFGLQSYYGFHKYIGQGSEAYGGPIIAVGLVRELIPIL
                     VGLMVAGRVGSSMAAEIGTMRITEQIDALFTLGADSVRYLVAPRTAAAFLMLPCLTLF
                     GDLIGILCAEFYNVVLMGVNKTVYWRNTVTYLDLWDVGSGLVKAAVFGVLIAIIGCWQ
                     GLETEGGAEGVGRATTRAVVIASIIILISNFFLSELLPSSL"
     gene            1246..1992
                     /locus_tag="PKV64_01610"
     CDS             1246..1992
                     /locus_tag="PKV64_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN15806.1"
                     /translation="MIRFNGVHKSFGPKRVLTGLDLEIRTGETLAVIGQSGSGKSVLL
                     KILIGILAPERGKVFVDDVEVTALGRDERQATARKFGMLFQAAALFDSLNVAENVAFG
                     LRRRMRLSEQEIAGLVADSLAKVGLRGIEKLMPHELSGGMRKRVGLARAIAYRPEIIL
                     YDEPSTGLDPIRADAINDLILLLQKEMGVTSIVITHDMVSAYKVADRIAMLYGGKIIA
                     VGPPEEIRHSPDPVVQQFLQGRAEGPIKDF"
     gene            2004..3287
                     /locus_tag="PKV64_01615"
     CDS             2004..3287
                     /locus_tag="PKV64_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPN15807.1"
                     /translation="MSRKTRVGLFVGGTLAVMAVFIMLLGDIGYVFKKRGYTLNVDVP
                     TAAGLDAKAVVKMAGVKIGFVRDIQLSGVRARVVMNIDNGVRVPVDSTAAFSTLGLLG
                     EKYVEIDPGESAEFCADNASIAGSQKPGFDQVGEMLSSVGRELKAVSASLSDVLNEEN
                     RAKFKRALEGAAGAAAELETILTVNRSEFERLIRGANETVDGVGRTVADVSADLKKSL
                     AVLQETIAENREPLKADLEKIGEAAAKLRAILEKIERGEGTAGKIIQEPGLYDEAAEV
                     LSSARKAAGLLSGVKGYADFQTGYYGTSRLVRAGLAAGIRPANGSFVEAGIVRDPWAG
                     RFVFSLQGGLRLGRFAPRLGFIENELGFGLDYLGGDLWGLSLEGFNFNREESPRFRLT
                     GKIFPTRSLYLVAGADDFTLAGRREFFFGLGLALR"
     gene            3284..4645
                     /gene="radA"
                     /locus_tag="PKV64_01620"
     CDS             3284..4645
                     /gene="radA"
                     /locus_tag="PKV64_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPN15808.1"
                     /translation="MKNKSVFVCQGCGTKSPKWMGRCPACGEWNTMVEEAEVEEAPLV
                     EQAFAPAEPIPYQDVKNLAKPRARVEIKEVDAVLGGGVVLGSLVLIGGEPGIGKSTLL
                     LQMSRDFAAGRTPVLYVSGEESLDQIKLRGERLGVGGGPLFLLAETNLERILAQAEKL
                     RPGVLVLDSVQTVFSSKLSSSPGTISQVREVANQVFRFAKRNGVPAFLIGHITKEGSL
                     AGPKSLEHIVDVVLLFEGERDHSHRVLRALKNRFGPVSELAIFEMTAAGLEAIPDPSA
                     FFLRERPKNESGSVVVATIKGTRPLLIEIQSLVSPTLFASNPRRMTVGLDHYRTSMLL
                     AVIERKLGYTFGTEDIYLNAAGGMEIGEPAADLGVVLGIVSCLMNRPVPAGLAVFGEV
                     GLSGEIRSVGQAAARVREAAALGFGTILLPEGNVAGLAKEKIDGVRLLGAGTLRRAVD
                     LVF"
     gene            4696..5685
                     /locus_tag="PKV64_01625"
     CDS             4696..5685
                     /locus_tag="PKV64_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPN15809.1"
                     /translation="MGIFLFRIFFVAVTAAAGYFVPPFGLDRIPAAAVAGFLAAATLF
                     LESRVRRASFTVIWGSVVGTLVGVILGWILGAAYQSMTSDPGTAAFVRVMFLLLLPAA
                     GFLIGIRKSEWLDPSYLFRFFREKNNGRIFKILDTSVIIDGRIAEICEAGFLEGTLVV
                     PQFVLKELQMVADSPDGLKRQRGRRGLDILDHLQKSKTANVMITEMDFPEIREVDTKL
                     IEVACRLEGKIVTNDVNLNKVARLRGVAVLNINEMANAIKPVVLPGESMMVYLIKEGK
                     EKDQGVAYLDDGTMVVVDNARRMIGRNVDIMVTSVLQTTVGKMIFGRYNDEAK"
     gene            5682..6362
                     /gene="ispD"
                     /locus_tag="PKV64_01630"
     CDS             5682..6362
                     /gene="ispD"
                     /locus_tag="PKV64_01630"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPN15810.1"
                     /translation="MKRTAAVIVAAGSGARFGGPKQFALLGGRPLVEWSCAAFQAHPR
                     VEDIILVLPETAAGAEYLRRFPKIRAVAAGGPRRQDSVAAGVSRLDERRTGVILVHDG
                     ARPLVSAGLIDRVIEAAEKRGAAVPAVPCRDTLKEIDGGRVVRTLDRGRLGRVQTPQG
                     FASALLRRALAAASAEGISGTDEAALVERLGEEVAVVSGDPENIKITTPLDLIAAEAI
                     RHENRIGI"
     gene            6340..6834
                     /gene="ispF"
                     /locus_tag="PKV64_01635"
     CDS             6340..6834
                     /gene="ispF"
                     /locus_tag="PKV64_01635"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPN15811.1"
                     /translation="MRIGLGYDLHRLTAGRPLYLGGLRIPHSRGLLGHSDGDVIIHAV
                     IDALLGAGGEGDIGRLFPDTDPGLKNVRSTELLRDVVSRLRRKRLRIVHVDAVVVAES
                     PKLGPYFEKMKDVLAPLLGLPRDRLGLKAKTNEGLGEIGRGRAIACWAAASLRPVSGR
                     KPKE"
     gene            6890..7306
                     /locus_tag="PKV64_01640"
     CDS             6890..7306
                     /locus_tag="PKV64_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15812.1"
                     /translation="MRKNVFIVCVVLFSSLAASAAFDLGGSWELTTVTPRGERVSTVV
                     FVQEGERLTVTVKRPGRDGRLVEMTSEGTFKGETIEWKETRRGPEGREMTVAYKGTVA
                     ADGSLKGTMEFSGGRGFAGGEGAPPVFEWKAVRKAS"
     gene            7520..8089
                     /locus_tag="PKV64_01645"
     CDS             7520..8089
                     /locus_tag="PKV64_01645"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPN15813.1"
                     /translation="MDEKTLVELSRNGRTDAFEKLVRMYDRKIFALAYGFVRDRATAD
                     DLAQEIFLKAYQSLPSFRVESGFGTWIYRIAVNHVKDHLRKASRRRTVSLDDVGDGPV
                     SVDEETGLRLREREEEERRKEVFRVMETLPPKFRAILTLRDVEGLPYEEVGRILGIAP
                     GTVDSRLFRARRMLRKKMEPYLEAEGGRP"
     gene            8086..8568
                     /locus_tag="PKV64_01650"
     CDS             8086..8568
                     /locus_tag="PKV64_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPN15814.1"
                     /translation="MSCRRVERCLLAAFDRDLSAPDRRSMERHLAECPACRKRAEAHA
                     EIRRRLSGSAVPEPLPRFWERLESRMTASGPALPAAWARLWAEAIPISLSLIAGFVLA
                     TLFLFPSSRSSDLSGSEALLLTEGNPIAETRAIIEEGRRDVRDLTVLFAADEQPAGKR
                     "
     gene            8574..9071
                     /locus_tag="PKV64_01655"
     CDS             8574..9071
                     /locus_tag="PKV64_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15815.1"
                     /translation="MKQTRWGIVLSLGLAFAAGAAAGMFIDRVFFFPRGHHRPGPSPD
                     QWEKDLGLSGEQKARMEEIFKKNDARIDALRSDFYKHLGEIRAEIRKEIDTVLTPEQK
                     AKQSAMMEKFRKSRSKDSGSRPSGPGRPPDGGFSKENKDEKESAGGSGDSSRYRRPDY
                     RHFLY"
     gene            8995..10326
                     /locus_tag="PKV64_01660"
     CDS             8995..10326
                     /locus_tag="PKV64_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPN15816.1"
                     /translation="MKKKVLAGAVILAAIAVLIIGISFIKKGKNNALPYRTEALTRGD
                     IESLVVTSGTVNPVDIVEIGSQVSGKIVQLHADFNSVVTAGQVVAEIDQEILKAKVQQ
                     NEASYLSRQASLEQAKVNLENARKKWERTQDLFDRQLVSHEERETVEANYVTAKVNLQ
                     VAEASLAQQKSALDSSRVDLSYAIIRSPIDGTVITRNVNIGQTVAAGFQTPILFKVAS
                     DLTKMQVECPVDEADIGRVKEGQAVRFSVDAFQGEVFTGEIQQVRNSPTTTNNVVTYT
                     TIIKTSNPDLKLKPGMTATVSIITGEARGTVKIPRAALKFTPSLSEEEMKKMWEEARA
                     KREALRGSGPRPEGSGPRPTGPVTEEMRKQFAAARSRDGFGQVWVLEESGKIRPVPVR
                     TGITDNTYAALLNGDLSEGMKVILGTGGAAASTQAQGSAMGGMMMMGPPRR"
     gene            10347..11030
                     /locus_tag="PKV64_01665"
     CDS             10347..11030
                     /locus_tag="PKV64_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN15817.1"
                     /translation="MNADIIRLENITKTYIVGETEVRALRGVSNIIPTGDFVAIMGAS
                     GSGKSTLMNIIGCLDKPTSGRYFLDGEDISTLSRDALAGIRNRKIGFVFQSFNLLPRT
                     TALENVELPLIYANVSHKERLARARASLAAVGLKGREAHKTNQLSGGEQQRVAIARAL
                     LNKPRLILADEPTGNLDSKTSDEIMNVFERLNHEDGISIVMVTHEPDIGARAKRRLHM
                     KDGLVVRED"
     gene            11046..12272
                     /locus_tag="PKV64_01670"
     CDS             11046..12272
                     /locus_tag="PKV64_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN15818.1"
                     /translation="MNVFRILRVSLRALARNKMRTFLTCLGIIIGVAAVIAMVSIGAG
                     ARAAVESQFASMGTNLMFVTGNWRMMGGARTQTGQFNRLTAEDAKAIGQLPSVEAVSP
                     SVSTRAQLIYGNNNWNTMVQGTGESYPLVRKWDIEDGAFFDESMVRGAAKVCVIGADI
                     KKNLFEEDEDAVGKTIRISRVPFLILGVFKSKGESGGFGSRDDFVCVPYTTALNRLSR
                     SEYLQSIDVSAVSQAKTKEAQDEITELLRIRHRIPDGGTDDFVVRNMSEVAEGAAEAT
                     GILTILLGSIASISLLVGGIGIMNIMLVSVTERIREIGIRMSIGARPRDILLQFLAEA
                     IVLSLAGGLLGIILGIGVSKVLKYIPVFASITTMVSWSSVLLAFAFSGSVGIFFGFYP
                     ARKASKLDPIEALRYE"
     gene            12301..13869
                     /locus_tag="PKV64_01675"
     CDS             12301..13869
                     /locus_tag="PKV64_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPN15819.1"
                     /translation="MHIPRRIAVPAALILLAATAGAQETTRSLSLTLEECLVRALRNN
                     LSIQVAVLTPQGSALSVQKAREKFLPNFSFGLTKRNSSSPSFSWLESQDETLTKYGNY
                     SAQITEVIPLGGTVTLGVSSAMTDTNQTGTTINPRYNSQLQLSFSQPLLRNFGLKMTQ
                     MEIVIARNNLDVSEIQLHKTIQDTIFSVIQSYWNLVYSIENLKVQRKSLELARDFLAK
                     NQRSVEIGTLAPMDILAAESEVATREAGILAAEAAVKANEDQMKNLLNFTPEEEQGLR
                     EIVALDSPRFEERLIEVDEALAVAMEKRPDLRISRINLKTQNLNLDYARNQLLPALSF
                     NASYASPGVSGTRLIYDSEYFGDIIGTIPGYRSESWNDVFGFRYENWSVALTLDIGLN
                     TVLSRAAYAQAELATQTAGLGLKNTERAAVLEIRNAVRTVQTAFKQVQAYKIARDLAE
                     KKLAAEEEKLRLGLSTNYTVLQYQRDLTTAQVQELKSVIDYNIALADLDRSMGTLLEN
                     RNIRIADALGGGSY"
     gene            13914..14129
                     /locus_tag="PKV64_01680"
     CDS             13914..14129
                     /locus_tag="PKV64_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15820.1"
                     /translation="MPGRPAMARLRFDGRMVIRYEGRGLGFREVAERPQRVQAVPVIR
                     PKPPEFIPPPTRWWKVYRGPAETEDLR"
     gene            14814..16088
                     /locus_tag="PKV64_01685"
     CDS             14814..16088
                     /locus_tag="PKV64_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPN15821.1"
                     /translation="MSRIFRPLKNRRRAAVLSLGLAFAAALAAAAAGGRDEAEELSLA
                     GAVELALAGHPEILLVREEAKAAAARRRQAEARPDPRLTFGTAGIPWTLKAGEVETEY
                     ALGVEREFEYPGLRAARVEIARRDEEAASLEIERTGLLLAARVKKAYHQAVFAEETRI
                     ALESLGAVIDRLTDALLVRFESGEAAYADVLRAKVEKARLQNRLIEARRDESSARTGL
                     LLFLGFSPDKPVRLTDRLAVPPLEASLDRVLAEAGKHRPSLRLARLRRSRAEAEVRMA
                     SLAAKPGFEAGLFVPSKNIKGWGFSLGISLPLSSSRTGGLQAEAAAAREKSLIETVAV
                     ERRLDILVRAAYREARAAEEQVEVFEKRLLGEIEAEIENGLTQYRLGRIEAYALLDLF
                     RSLAEARLEHLQARFLYAVALAGMDAAGEDGE"
     gene            16102..17229
                     /locus_tag="PKV64_01690"
     CDS             16102..17229
                     /locus_tag="PKV64_01690"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPN15822.1"
                     /translation="MNRTKITTILALAAAIAVFPACRRSLLPEHAGHDAQENGRVPAG
                     TVILSIAAAADGGISAARVETAVLAGRISVPGELEFNARRLAEVSARVEGRIEKAAAV
                     AGDRVVPGQILAEIYSREFLAAQAEVLQAAARAARLRDGPEGPAAEAFLRAARRKLLP
                     LGWGDEEIDALLEAGEPVPFLSVRAPLAGTVIEAPALAGAHVEPGDPLFKLADPASLW
                     ARVHVFEKDLAAVRPGSSAVLRTQAFPGREFPGRLVLLGPVMDEKTRTVEGRIEVGNA
                     DGCLRAGMYVEAALASGRERTAVVVPASALQEFQSLPVVFVREGETTFRLRPVEIGDR
                     EGGLVEIVGGVSAGEWVVTTGGFLLKSELLKSSLADEHGHD"
     gene            17216..20371
                     /locus_tag="PKV64_01695"
     CDS             17216..20371
                     /locus_tag="PKV64_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HPN15823.1"
                     /translation="MDTIERLISRSLRNRLLVLIGVALLVGFGIWSFLKIPLDAFPDV
                     TNIQVEVLSSAPGLSPLEVEKFVTFPVETTLQGLPRLTQLRSVSKFGLSVITVVFEDG
                     VDIYFARQLVLERLIEARERVPQGIEITMGPVSTAMGEIYQYTLEGPVPEGEDEEARA
                     ERLTELRTVQDWILAPLLKSVPGVSEINSFGGHIRQYEVTVDPDRLLKYGLTVADAAA
                     ALERNNLNVGGSVLEDAAGQALVRGVGLLRSASDMERIVLKSEAGTPVFLGDVAAAGK
                     SRAVRQGAAVKDGKGEAVGGVVMMLRGANGREVVRAVEARVAEVNAGAVLPSGLQIVP
                     FYKRSDIVQASLHTVSEALIIGGIFVLLVLYLFLRSFRGAFIVILALPLSVLFTFIAM
                     RLGGLSANLMSLGGLAISIGMIIDATIIQVENVQRHLSESGPGRTKTETVLSAVLEVR
                     KPSIFGELIIALTFIPIFALQGIEGKMFAPLAFTHVIALCASLCLSLLVIPAFCRLLL
                     KPQGEKRIFLVEAARKAYLPLLRWSLDHKKTVFAVSLGLLAASAVLIPRLGTEFMPIM
                     DEGAFDMDVQFAPGISLPQSLAMNRKVEEKLISFPEIETVVGKTGQTGIALEARGVEK
                     TGYVGILKPRRQWTSARSRGELTEKMREAVSTIPGMAFSFSQPIACRIDELVAGTRAQ
                     LIIKLFGEDLDTLTAKAGEIAAALRRLRGASDLVVESVAGQPYLSVLPDRERIARYGL
                     SVEDVQTLVETAVGGRTVTRIYEGERAVDVQLRYAEKRRGSAEAIGALPLRTERGEIV
                     PLSQAAAIVRSEGPSQISREAGRRRIGIECNISGRDLGGFVAEARREVARSVSLPAGY
                     FLSWGGQFENQQRAMRRLGVILPVTVGLILLLLFLTFNALRPALLVLFNLPFALIGGV
                     LSLYLSGLYLSVPASVGFIALFGIAVLNGIVLLSAVAQRREEGLAAREAILQGCLNRL
                     RPVLMTATITICSLLPLLFAQGPGSEIQRPLAVVVVGGLATSTLMTLLVLPALEAAFG
                     EDRMRRGGRGRRPCPEPADGL"
     gene            20379..21032
                     /locus_tag="PKV64_01700"
     CDS             20379..21032
                     /locus_tag="PKV64_01700"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPN15824.1"
                     /translation="MKDRAGRGAILVSAVLLLMSCAPPGRGVIVFCAGDSLTKAAYPD
                     FLRRELNRDGIAARVLNYGRSGYTSGEYLKFLVKFRPKLIEEKPDVVLLQLGTNDVRI
                     DHDATPTDAFKENMRQILAVFRTFRSRSGKIPLILMATIPDIPETPPNFDASSARRVR
                     AEINPAIREIAAAEGIAVVDQHAVFLRRPDLLPEVHPVREGYRLMAEVWREALRPRY"
     gene            21281..21703
                     /locus_tag="PKV64_01705"
     CDS             21281..21703
                     /locus_tag="PKV64_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HPN15825.1"
                     /translation="MNRRLIRPHLNEIKENLKGQETPVPHHGRPSSAGHSGHASSRGP
                     YASQTQSQPKKMYPPTDTGAENYYYLKQMGKKTPMAVVFQDGEILEGYVEWYDRNCIK
                     LNRNGAPNLLVYKNAIKYLYKLGEGKESPEEDGEPERK"
     gene            21700..22677
                     /gene="pdxA"
                     /locus_tag="PKV64_01710"
     CDS             21700..22677
                     /gene="pdxA"
                     /locus_tag="PKV64_01710"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HPN15826.1"
                     /translation="MTFPRIGISIGDPAGIGPEVTLRALFFSGALPPARYVLFGPRSF
                     LEKEAAAFGLSTEIPSWKTDESCAGAGVYLWDLPGPREPVKKGEPGASAGRASFAWFE
                     EAAAAARRGEMDALVTAPVAKVGWNLAGIPWRGHTEYLESFFPDAVMSFWSEKLTVAL
                     LSHHLPLRDALARVNRPALARLFRILRECPPARAEGPAFEILVAGLNPHAGEGGILGT
                     EERDVIVPAVAEARAAGTPVRGPFPPDVVFRLALGRPEKIVAALYHDQGLIPFKMIAF
                     ETGINVTLGLPFIRTSPDHGTAFDIAGRTPADPASMTEALRRAARSASA"
     gene            complement(22663..23115)
                     /gene="smpB"
                     /locus_tag="PKV64_01715"
     CDS             complement(22663..23115)
                     /gene="smpB"
                     /locus_tag="PKV64_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPN15827.1"
                     /translation="MKVLAVNRAARFNYEILETFEAGLVLQGSEVKSVREGRISLKES
                     FADIRDGEVVLVDCHIHPYEAANRWNHDPRRVRKLLLHRREIKRLTGKVQERGLTLIP
                     LQVMLNDKGLVKIEIALGKGKKIHEKKEALKERDINRQIRAALKSGGR"
     gene            complement(23112..24353)
                     /locus_tag="PKV64_01720"
     CDS             complement(23112..24353)
                     /locus_tag="PKV64_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPN15828.1"
                     /translation="MTLSLILLFILCIILSAFFSSSETAFIAVKPHTLDYLETKGSPR
                     AGRVKKILSRLEDFLSAVLIGNTLVNAAAASLATALAASLIPNRNTAVLAATGGTTLI
                     LLLFSEINPKIYAARHPDKLALFFAPVVRLFLVLFYPLVAVFTFVSRRLFRSGAKTSE
                     ALSRAMAEEEAKALLHSGIKGLPEHRKAMITEIVDLARRPVKEIMTPRPRIKAIEASS
                     SREQILKTVVAEGFSRFPVYRGRMDHIEGLIYTKDLIGYLAHDRPFDLGAVLRPPFFL
                     PESASIEKVLLQMQDEGVQMAFLVDEFGNMEGLVTIEDIMEEIVGEIRDEYDDREEDW
                     HAPLEDGAVLVKGSAGINDINKTLGWGLPESSEYTTLAGFFLQEFGRIPREKDVLLHN
                     GRRFIVVKMNKRHISQIRVET"
     gene            complement(24363..24881)
                     /locus_tag="PKV64_01725"
     CDS             complement(24363..24881)
                     /locus_tag="PKV64_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPN15829.1"
                     /translation="MECFKIGDKVIYPNQGLGLVVDVQEQAYNGEKFPVYHLRLLSSN
                     TLVMVPFSSCQEIGIRKLIAEGSVKDIFDFLKNGEIEVSMNWKGRYKEHIGLMKSGTV
                     LDMVAVLKSLHYLSLIKPLSFREKKMMEKARELVVSEIAQVSSMPAAQIEKKIASILS
                     TCFKDVKVGLDV"
     gene            complement(25064..25267)
                     /locus_tag="PKV64_01730"
     CDS             complement(25064..25267)
                     /locus_tag="PKV64_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPN15830.1"
                     /translation="MNRILETLVSTGPIRSVPPSTLPTLDELVGEYILMLLALTHHNI
                     SQTARILNISRTTLYNRLRRQSS"
     gene            complement(25270..25818)
                     /locus_tag="PKV64_01735"
     CDS             complement(25270..25818)
                     /locus_tag="PKV64_01735"
                     /inference="COORDINATES: protein motif:HMM:NF039183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HPN15831.1"
                     /translation="MKKTVLAFLITFPLLSGVSLAFPTLNETPLLPSGTLSALALPSE
                     PAPEPDFSSITLNPRLVVLASAQSLIEPSLRRSRFADSVFDATLISMVALNIADYVST
                     RECLKFPHLSEGNPIMKPFVKNDLAFAAVKGGLTLATYFGTKALFKKSKPLGWIASLA
                     SNLALSYVVSNNFRLLREARSR"
     gene            26200..26340
                     /gene="rpmH"
                     /locus_tag="PKV64_01740"
     CDS             26200..26340
                     /gene="rpmH"
                     /locus_tag="PKV64_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010900190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPN15832.1"
                     /translation="MMKPSFNPNNRKRKKAHGFLVRMSSAGGRKVIKRRRAKGRKRLA
                     AE"
     gene            26337..26693
                     /gene="rnpA"
                     /locus_tag="PKV64_01745"
     CDS             26337..26693
                     /gene="rnpA"
                     /locus_tag="PKV64_01745"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPN15833.1"
                     /translation="MSDSYGPRERIRKKSDFSALYQRGRSFRSRYFTIVYLSNGLGYP
                     RMAAVSSRKVGNAVRRNRARRRARELFRRNKESLKMSLDMLLITRAECADAPWPQLCE
                     QYAAFLASLAREKSLR"
     gene            26690..26899
                     /gene="yidD"
                     /locus_tag="PKV64_01750"
     CDS             26690..26899
                     /gene="yidD"
                     /locus_tag="PKV64_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006696491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPN15834.1"
                     /translation="MKRLALALLRLYKRTVSPLLGTHCRYYPTCAEYACQAIEKYGLI
                     RGTGLGLKRLLRCHPFHAGGLDPVP"
     gene            26915..28591
                     /gene="yidC"
                     /locus_tag="PKV64_01755"
     CDS             26915..28591
                     /gene="yidC"
                     /locus_tag="PKV64_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPN15835.1"
                     /translation="METRRIILAIVLSLGVLLLWQYVFVKKAPETAPPPQPVAAQAAA
                     PAPETETAGRPEAETAPAEERASDAAEALNAETEEEIIVSTSLYTARWTNKGGVLLSW
                     KLHHHKNEKDEALELVPAAAEASGVQPLALLEALDPSRATIEELRSTPLNTALYEATG
                     GNLSLKDGEKGVLRFRYADGRGLEAEKTFTFTGGRYDVGAAVTVRRGGRPADVRIVWG
                     PGCGNPSEAELKQRFGAGAGASFLAGTKVYRVEEKKYKPESSVFNFLTWSAYDDNYFA
                     TIFLPETASGSASFLRFENGKTPFFFLAASAPRTIFIGPKEMGVLTALGSETKRVVRY
                     GMFGFITEILFVSLRAIHKVVPNWGFAIIVLTILIKILFFPLTYSSSKSMARMADLQP
                     KIKALRAKYKKAKTDIEQRRQMNEEMMKLYKEHGVNPAGGCLPMLIQLPIFWGFFRML
                     TVAIELRHSPWILWIKDLSVHDPTYVTPILMGVTQFISQKMTPTSADPAQARMMLIMP
                     VVMTLFFLNFQSGLVLYWLTSNVLQIGQQALMNRMMQKKKVVSHGKSRKK"
     gene            28569..29216
                     /locus_tag="PKV64_01760"
     CDS             28569..29216
                     /locus_tag="PKV64_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HPN15836.1"
                     /translation="MENQEKNDAVTAVEEIPEFKGKNLEDAILHAEHKLKVPRTEFEY
                     EVVTEKTRLFGLGKEVVIRARVKRFAKNGDLTVFLDRLMNVFPLDLTYEIKTKEDISF
                     VIFGGADRQILLWKDGALLLAIQHLLNKISDRKVQVDCEFFRKRKEKSLRDYALDVAR
                     RVADSGRNEILDLMNPYERRIVHIAANQVPGITSESLGEGFLKRVKIYPLSKRAR"
     gene            29251..30630
                     /gene="mnmE"
                     /locus_tag="PKV64_01765"
     CDS             29251..30630
                     /gene="mnmE"
                     /locus_tag="PKV64_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPN15837.1"
                     /translation="MYDLSLSETIVAVSTPPGCGGIGVVRLSGPAALKIARTLFRPKK
                     DRRGGEPGRAVLGEVVDPADRRPFDEGFLLHFRAPRSFTGEDVAELHLHGSPVILEEV
                     VRLAVRRGARPALPGEFTLRAFLNGRIDFLQAEAVDDLVRSASLARAKSAFRTVNGAL
                     SRKAAAVRREIIGWVARLEADIEFPEEGRPAAKKHYIDKIDKIYLEVDRLVRSYEMGQ
                     ALGRGVTIALVGRVNSGKSTLFNGLLEEPRAIVTPFPGTTRDFLRETVRINDASFLLV
                     DMAGLGRAGSPIEREGIRRGKKIAAEADGLLLLLDGSRRLSASDAALIESYRSRKIIL
                     VLNKTDLPEKTTPAEVTRRFPDLPVAAVSALRGDGLDVLRKMMYDVFFPLIPGRDIIV
                     LRERDKLLLEEIAARLLRAKEMIASNQPIELAAEEAREIVTLTGRLTGEIRPDDILDD
                     VFSRFCIGK"
     gene            30736..32601
                     /gene="mnmG"
                     /locus_tag="PKV64_01770"
     CDS             30736..32601
                     /gene="mnmG"
                     /locus_tag="PKV64_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013582043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPN15838.1"
                     /translation="MADESFEIIVCGAGHAGCEAAAAAAAMGCSVCLITTHFETIAQM
                     SCNPAVGGLAKGHLVREIDALGGLMGLLADATGIHFRLLNRSRGAAVQAPRVQCDKSR
                     YREAMKEQLEKIPGLSLYQGIVVGLDIVGSRIRGVVCGDGRSIEAEAVILTPGTFLNG
                     LIHVGQASYPAGRANEPPSVELAAAVRKAGLRTFRLKTGTPMRLDGKTIDWSAFEAQP
                     GDDEPVPMSLRTETRLENKVVCYLGYTNEKTHAVIRRDLDKSPLYAGRIKGLGPRYCP
                     SIEDKVVKFGHHPRHQFFLEPEGLATSEIYVNGLSSSLPYETQRDILRTIPGLTEAVI
                     LRPAYAVEYDAVSPTQLKPTLESLPVEGLYLAGQINGTSGYEEAAAQGLAAGVNAVLK
                     LRDKKPFVLRRDQAYIGVLINDLISKGVEEPYRLFTSRAEYRLGLRIDNADSRLSPYG
                     RELGLLSERDFVRFQKKEKRLSSTLAFLEKTKITPAGKARISLKEYLRRPDIEFENVL
                     EYVPMPEALSPEETRRIAAEVKYEGYLRKQEVEIVRLTKMEAMRIPAGFKPSGVPGLG
                     REAVEKMNKHKPKTIGDMKRIPGLTPSDVMNVFIHVSAGNVPRGTSPGKPTREDE"
     gene            32594..33349
                     /locus_tag="PKV64_01775"
     CDS             32594..33349
                     /locus_tag="PKV64_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPN15839.1"
                     /translation="MNKIIAVANQKGGVGKTTTSINLAAALALKKSRVLLIDLDPQGM
                     ASAGLGKPKADSAGIYQAMMNGRDIMECISGTEMDNFYLCPCSPELMGVEAEMFKAEF
                     RENKLKNALEKARPRFSHILIDCPPSLGFLTINALTAADSVLIPVQTEFFCMEGIPDL
                     LHTLNEVRTYFNPGLRIEGVVLTMADDRTNLSRQVADEVRKSLKELVFRTVIPRSIRL
                     AEAPSFGKPVILYEPRSRGAEAYLSLAQEILEK"
     gene            33346..34188
                     /locus_tag="PKV64_01780"
     CDS             33346..34188
                     /locus_tag="PKV64_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPN15840.1"
                     /translation="MKKALGKGIKAFIPEEYGILKEESLTEMDIDRLSPSPFQPRMKF
                     DEQAIEELAQSIRESGILQPIIAVPAGGGYNIIIGERRWRAAQKVGLRKVPVIIRNIS
                     KERQMEASIIENLQREDLNPIEVARYYKGMVEVLGYSQEEVAEKVGKDRTSVSNTLRL
                     LNLPEAVQSMLLQEKIAMGHARAILGIEDPKAQVEAARWAADSGRSVRETEDYVSRRR
                     KKKTPVRAKLDPDLQNLQEDLLQHLGAKVVISGTPKKGVIKISYFSLGELNRIYELIK
                     GIRS"
     gene            34185..34583
                     /locus_tag="PKV64_01785"
     CDS             34185..34583
                     /locus_tag="PKV64_01785"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPN15841.1"
                     /translation="MKEGKEKRVSNRAELSGLYDTGTEFQGVLKFEGSFRIDGYFKGT
                     IESEDTLIIGEEGNVEAEIHVGHAVVNGEFHGRIRCEKKIEVHGRGRIFGTIIAPKIM
                     IDESAYLQAECRTEEAPVSAEPAAEDKESE"
     gene            34580..35308
                     /gene="kdsB"
                     /locus_tag="PKV64_01790"
     CDS             34580..35308
                     /gene="kdsB"
                     /locus_tag="PKV64_01790"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPN15842.1"
                     /translation="MKRKVLAVIPVRFGSTRFPGKPLALIAGKPMIQRVIEGVRVCRS
                     IDRVITATDDNRIFETVRALGAEAVMTSPDHASGTERVAEVAAALDYPVVLNVQGDEP
                     LVDRAALDGLAEALQDHRLPMASVMARIEDISAAADPNRVKVVVDASGHALYFSRALI
                     PAGASDFFFQHVGLYGYQRDFILRFGRLPLSRLEQIERLEQLRVLENGFRIRMIEIPR
                     PTLSVDVPEDIMRVEKFLKESGSR"
     gene            35305..36915
                     /locus_tag="PKV64_01795"
     CDS             35305..36915
                     /locus_tag="PKV64_01795"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPN15843.1"
                     /translation="MTKFIFVTGGVLSALGKGIAAASIGRLLESRGFKLTIIKLDPYL
                     NVDSGTMSPYQHGEVYVTDDGAETDLDLGHYERFTSTQTTKYHNWTAGRIYDIILKKE
                     RRGEFLGKTVQVIPHVTDEIKNALKSVAESNDIVIAEIGGTVGDIESLPFLEAIRQMR
                     LSCGADHTLFVHLTYVPYVQTSGELKTKPTQHSVRELRAIGIQPDILLCRTDRSLGPE
                     MKAKISMFTNVPENAVITARDVENIYEIPLVFAEEGLDAIILRQFGLTSRTRRLSQWK
                     TFVRRMNQPRDEVDIAIVGKYAGLEDSYLSLKQALIHGAVAGDLKAVIEWIEAENVEK
                     GKLEKILGTKDGILIPGGFGQRGIEGKIRAVTFAREHNVPLFGICLGMQCATIEFARN
                     IAGLKGAHSAEFDEATPAKIFFKWRELKGVTDLGGTMRLGRYLCRLEKSSLAYKIYRN
                     EEIWERHRHRFEFNPAFEKPLREAGLRFSGKNPDYGLAEIVELPGHPWFLGCQFHPEF
                     KSRPLRPHPLFKSFIGASYAHRQEKRAA"
     gene            36884..37723
                     /gene="kdsA"
                     /locus_tag="PKV64_01800"
     CDS             36884..37723
                     /gene="kdsA"
                     /locus_tag="PKV64_01800"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPN15844.1"
                     /translation="MPIAKKSARPEAARSAVPPAGLFLIAGPCVIESRAKTIALARKL
                     AAVAARLDFPLLFKASYDKANRTSLRSFRGPGLRAGLEILRAVKETAGIPVLSDVHEP
                     AQAVPAAEVLDVLQIPAFLCRQTDLVLAVARTGKPVNVKKGQFLAPGDMMHVVEKILS
                     TGNARIMLTERGTCFGYHDLVVDMRSIADMKRLGFPVVIDASHGVQKPGGAGGASGGE
                     RRFIPALARAAVAAGADGVFLEVHDDPARARSDKDNAWPIDEVEALMNVLLRVYGALG
                     EPR"
     gene            37720..38691
                     /locus_tag="PKV64_01805"
     CDS             37720..38691
                     /locus_tag="PKV64_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HPN15845.1"
                     /translation="MTKEDIIRAGRDVLDVEARAVARLRENIGEPFVRAVNLLYRTKT
                     RVIILGMGKSGLVGRKIAATLASCGTPAIFVHPAEAGHGDLGMILEGDVVIAVSYSGE
                     TREIIDLLGYIKRLGVKLILITGAPRSHIARYSDIVLEARVSKEADPDGLVPTASSTA
                     ALALGDALAIAVMKRKGFTERDFASVHPKGALGKRLLRIETLMHKGRAVPRVGPSALM
                     SEAIEEMTAKKLGMTSVVDEAGRLAGVITDGDLRRMIRKHGATLLSRTAGECMTTGPL
                     TVTRTDLAAEALNVMEQNRITSLVVLAAGGKVEGIIHLHDLWRTEMF"
     gene            38691..39230
                     /locus_tag="PKV64_01810"
     CDS             38691..39230
                     /locus_tag="PKV64_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HPN15846.1"
                     /translation="MGKRGRARRVRMILADVDGTLTDGSLTPYPDGEEIKSFNTKDGL
                     GVLISRLAGLKVGFITGKNSKALEGRAARLRIDELRQGAIDKEPVFREILAKHGLRPE
                     EIAYIGDDLNDLAVLEAAGFSAAPADAVAAVRKRVDFVCRRRGGEGAFRELVDYILGA
                     QRKWSLVKDRFREISDHKI"
     gene            39266..40633
                     /locus_tag="PKV64_01815"
     CDS             39266..40633
                     /locus_tag="PKV64_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPN15847.1"
                     /translation="MNAKIFREYDIRGIVDVDLTPEVVQTLGRAIGTFFRSKGKKETV
                     VGRDCRLSSPAFAEAMAAGLRAAGCDVTDLGTIPTPLLYFAGYYKNMESGVMITGSHN
                     PPEYNGFKMTVGQDTLYGDDIQQIHRIIKNGAYAPAGKGSYRLYDIKPEYEAYVLGLF
                     KLARPLKVVLDAGNGTAGVVAAPLFRKLGCKVDELYCDMDGRFPNHHPDPTLPEAMQS
                     LVSRVKATKAELGLAYDGDGDRIGVVDDEGHLIWGDQLLIFFARDILKAFPGAVIISE
                     VKATKVLYDEITRLGGKAVMWKAGHSLIKKKIKDEDAKLAGEMSGHMFFADRWFGFDD
                     AIYASLRVAELVARSPQRLSEMLAALPKTYSTPEIRVYASEEVKFKIVEEVRRELSGK
                     YPVIDIDGVRAIMPKGWGLVRASNTQAVLVLRFEAETEEDLEAIQREIKASVERAIHK
                     LERPS"
     gene            40630..41718
                     /locus_tag="PKV64_01820"
     CDS             40630..41718
                     /locus_tag="PKV64_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPN15848.1"
                     /translation="MKSAVDLHAVIMAGGSGTRFWPLSRKRRPKQFLPIVSEKTMIEE
                     TYRRIRRIVPGRRVFTIADAPQSRSIRRLLPALPAANVLIEPRARNTAASLILATAHV
                     HRRNPEAVVAVLASDHLILDEPLFLKKLEAAAEAAFRTEGLVTFGVQPTYPATGYGYI
                     QFDRAGGRDFAGEPFYPVRAFREKPTREAAAAFLESGDYAWNSGMFLWRASVFARALE
                     RDSPAFFPFWRRTREALDKKSSKTLIRIFEEIPALSIDYALMEKAKGTLVCAGDFGWS
                     DVGAWSALGEIWPRDDQGNARRGDVVAVESRGNIICNPGKLTALIGVENTVIVQAGDV
                     LLVCRKDRDQDVKKVLDLLRGGGKTKHL"
     gene            complement(41774..42001)
                     /locus_tag="PKV64_01825"
     CDS             complement(41774..42001)
                     /locus_tag="PKV64_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15849.1"
                     /translation="MKFPQSWSAVSAASLAEWKTEYASFLAGSSFLKSGEAALAAEVM
                     EKTLLFGLAAIAADEPLEARFSIDSKILSCH"
     gene            complement(42004..42552)
                     /locus_tag="PKV64_01830"
     CDS             complement(42004..42552)
                     /locus_tag="PKV64_01830"
                     /inference="COORDINATES: protein motif:HMM:NF039183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HPN15850.1"
                     /translation="MKKTVLAFLITFPLLSGVSLAFPTLNETPLLPSGTLSALALPSE
                     PAPEPDFSSITLNPRLVVLASAQSLIEPSLRRSRFADSVFDATLISMVALNIADYVST
                     RECLKFPHLSEGNPIMKPFVKNDLAFAAVKGGLTLATYFGTKALFKKSKPLGWIASLA
                     SNLALSYVVSNNFRLLREARSR"
     gene            43174..43557
                     /locus_tag="PKV64_01835"
     CDS             43174..43557
                     /locus_tag="PKV64_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007086085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPN15851.1"
                     /translation="MKSESKRSLLPFTRSVCGLVRRIPRGKVISYGRLAALAGDGRQA
                     RQVSWILHSTSENEGLPWHRVINSKGRVSLPPERGGNEQARRLRREGVKIGPGGSIDL
                     DKYGWRMRWISGRKPPISERDLERF"
     gene            complement(43743..44567)
                     /locus_tag="PKV64_01840"
     CDS             complement(43743..44567)
                     /locus_tag="PKV64_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPN15852.1"
                     /translation="MNGDIPALFVTGKTLPEAWENAVLACWRGGAAVRTEYDKPGDPP
                     SRDCTMMWVVEEPFAEPRIHRAFPGGLEDLEIYRQEVVDGIHDHWIAPEEGKWTYTYH
                     KRLFAYETEGAVIDQIESLVEKLSRSGHSRRAQAITWNVALDPPTNDPPCLQRVWCRL
                     LPGDGGSFVLNMNTHWRSRDGFKAAYMNVFALTELQKSIAGRIAERSGKEIRLGRYVD
                     VADSFHIYGSYFRDFEGFLKLIEKRSFAERTWDSTYAEPMFVETREKLRREKAAKP"
     gene            complement(44594..44875)
                     /locus_tag="PKV64_01845"
     CDS             complement(44594..44875)
                     /locus_tag="PKV64_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15853.1"
                     /translation="MAEIVVHGSASFSERGPLPNTRFLHRVDAPAGESSQSPAASGRR
                     TLAGPRVTPIIYDLSTILGFGKIFKRQDISAFGDFFDDGIRAIDSPERL"
     gene            44855..45640
                     /gene="radC"
                     /locus_tag="PKV64_01850"
     CDS             44855..45640
                     /gene="radC"
                     /locus_tag="PKV64_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPN15854.1"
                     /translation="MNDDFRHPPGRPSPRAKNPPGPASAGHRSRLREKFLKGGLSGFL
                     DYEVIELLLTLGTPRRDCRESARLALREFRTLRGVLEAETHELRRIPGIGSANVFGIR
                     LIQEVARRFLKDRMLRRPYCRSAREVFEYLYHTMRDLKKENFKVLFLDAKNQILEEKT
                     LFEGTVDSSAVYPREIMKEALRFNAAALIFAHNHPSGDPEPSFCDREITKELVFAARV
                     MGLKVLDHLIIGNNRYFSFADHGLISDYDLLFHSVQAKTGAGG"
     gene            45727..47364
                     /locus_tag="PKV64_01855"
     CDS             45727..47364
                     /locus_tag="PKV64_01855"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPN15855.1"
                     /translation="MKPIVEIAAKLGLDPGNLVQFGTYKAKVDPAAGPPGRPAGKLIL
                     VTAMTPTPAGEGKTTTAIGLVDALTRAGKKALAALREPSLGPVFGMKGGATGGGKARV
                     VPGEDINLHFTGDLHAVSSAHNLLAALVENRLHFDGPAGLLDGRNVYWKRVVDMNDRA
                     LRQIIIGLEDTYGGMARESGFDITAASEVMAVFCLSRDLADLKARLGRIVVGCGLDLK
                     PVTAAALKAPGAMAALLRDALRPNLVQTLEETPVFVHGGPFANIAHGANSVIATGLAL
                     RLADYVVTECGFGSDLGAEKFFDIVVRSGAVPPPSAVVIVATLRSLKYHGGVKLDALL
                     QENIAAMDNGYANLQAHVDIVRQYGLPVVVALNRFPADSPAEIAAFTARAAADRLPYA
                     LSDVFGAGGPGGLDLAAKVVEAASSDAGEFRFLYPLEALIPEKLEAIARRIYGADRVV
                     LESPARKKLKRFEDNGFSGLPVCVAKTQYSLSDNPKLLGRPNGFPLTVTDLSISAGAG
                     FVVALCGDIMTMPGMPKSPAAERIDVSDDGVVSGILG"
     gene            47464..48561
                     /gene="recA"
                     /locus_tag="PKV64_01860"
     CDS             47464..48561
                     /gene="recA"
                     /locus_tag="PKV64_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPN15856.1"
                     /translation="MKDEEQSPRDVSRGKAIEAAVSQIEKQFGKGSVMKLGQRDAAQR
                     IEAIPTGSIAFDLALGIGGFPRGRVVEVFGPEATGKTTLALHVIAEAQRRGGQAAFID
                     AEHALDPTYAGKVGVDIDNLLISQPDYGEQGLEIAEVLVRSGAVDVVVVDSVAALVPK
                     AELEGEMGDAHMGLQARLMSQALRKLTAIVARSKTCFIFINQIREKIGFFLGNPETTT
                     GGRALKFYASLRVDVRRMTAIKEGDQVVGNRVKVKIVKNKMAPPFRDAEFDIIYGEGI
                     SREGDLVDMGANVGLVEKSGTWYSYKGERIGQGRENVKKLFKENTALAAELDADIRRK
                     VGLGQPEAEEKAPVEQKPARAPEKTSERRSR"
     gene            48617..49681
                     /gene="corA"
                     /locus_tag="PKV64_01865"
     CDS             48617..49681
                     /gene="corA"
                     /locus_tag="PKV64_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPN15857.1"
                     /translation="MARNASSRSKKSGLGPGSLVHVGRRKIDRPRITLIDYDNGHFLE
                     KEVAALEECLPFNLTSTVTWINVDGVHDPDVVEKLGQAFGLHPLILEDIMTTSQRPKL
                     DDLETSFYFVARMIELGGQGRELVTEQLSLVFGRNFVLTFQEAPGDMFDPVRERIRNG
                     KGRIRKLGPDYLAYALLDAVVDHYFVVLETLGDRIDTLEEELVRDPREETLHAIHALK
                     REMLIFRKAVWPLREVVAGIERTETPLIQPSTSIFFRDVYDHIIQVIDNVETYRDMLA
                     GMLETYLSSMSNRMNQVMKILTIISTIFIPLTFLVGVYGMNFEHMPELKWRFGYFLIW
                     GVMIILGLGLVRIFKRKKWF"
     gene            49713..49985
                     /locus_tag="PKV64_01870"
     CDS             49713..49985
                     /locus_tag="PKV64_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HPN15858.1"
                     /translation="MKVKQIVILVLVGLVIVLMIQNSVLVKFHLLFWPVYAPLFFLVL
                     GILAVGLIVGFLAAKAGRKTPPKPVSGREEKPAAATKTGPPTDPEK"
     gene            49982..50584
                     /locus_tag="PKV64_01875"
     CDS             49982..50584
                     /locus_tag="PKV64_01875"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HPN15859.1"
                     /translation="MKAFFTHLKTRIFRGFLVLLPLALSFLVLQFLYIAVDQRIARLF
                     ERWIGFSIPGFGLLLVLFVLYLLGLIASHWAGRWAIGVVDKASTRIPLIKTIYLLGKQ
                     LGAAFSAPEKQGLKRVVLVEHFRPGVWSIGFVMGSMREKESGRTLLRLFVPTAPNPTS
                     GFMLIVPESQVRDVDWSVPEAMNAVISGGLIGPEQVETRP"
     gene            complement(50581..53325)
                     /gene="uvrA"
                     /locus_tag="PKV64_01880"
     CDS             complement(50581..53325)
                     /gene="uvrA"
                     /locus_tag="PKV64_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPN15860.1"
                     /translation="MSGEDKIILRGVRVHNIKNVDVDIPLNAFTVVTGVSGSGKSSLA
                     LDTLYAEGQRRYIESLSSYARQFLERMDKPDVDTIEGLPPAIAIQQKAATKNPRSTVA
                     TVTEAYDFLRVLYARVGTVHCRRCNRPVTRDTIDAVVDKLLALPAGTPLSISFEAVIE
                     KSPAALKKLGFFRALVDGRIVPFDELPSLKGRTLSVFVDRLPADPDDRERLADSLETA
                     MKHGRGRARVQTEDGRSFAFSDSLECKECRIAYEDPTPNLFSFNNPQGACPECHGFGD
                     LAVLDEEKIIPDPAKSLEEGAIEPWTKPVSRGVMREMLREARRRGIPTRIPFRDLKAE
                     HRRFLFEGDEKWYGIKGFFDWLQAKKYKVQVRVFLSRYRKYAPCPACAQTRLNADARG
                     VKIGGLSIGEIVRLTVGEAAVFFHHLRLSPEEAKIAEKLVAEIENRLKYLIEVGLDYI
                     TLDRMTFTLSGGEAQRINLAAALSSSLVGTLFVLDEPSIGLHPRDNARLVRILQSLKG
                     IGNTILVIEHDPDIIRAADHLIDMGPRAGENGGEILFAGPLSTILKKPDGGRPPSDGA
                     SITARYLTGEKEIAVPRRRREPRGFLLVRDARAHNLKRIDIRIPLGVFACLTGVSGSG
                     KSTLLHDVLYRGWSGESRDAFADIRGLERIDKIICVDQSPISASPRSIPATYLKAMDG
                     IRDLFSQTREAKLAGLKPGTFSFNTKGGRCEDCEGAGRQIVEMQFLSDVILVCEGCQG
                     KRFKREVLEIRYRGKNIDDVLHLTVDDALEFFGDRPEIVKKLRPLQDVGLGYLRLGQP
                     TTTLSGGELQRIKLAFHLIHERDRNIFYLFDEPTIGLHPEDVSTLLRCFQRLVEEGHS
                     LLVIEHNLDVVKSADYVIDLGPEGGENGGTVVAEGTPEAVARSRASITARYLRKALRS
                     "
     gene            53409..53615
                     /locus_tag="PKV64_01885"
     CDS             53409..53615
                     /locus_tag="PKV64_01885"
                     /inference="COORDINATES: protein motif:HMM:NF023183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HPN15861.1"
                     /translation="MKKALFCGAVPFVALVAALPFVNRLRPVVLGLPFVLFWILGWVA
                     LTPAFLYLADRFLRKDKNGKDGKP"
     gene            53612..55042
                     /locus_tag="PKV64_01890"
     CDS             53612..55042
                     /locus_tag="PKV64_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPN15862.1"
                     /translation="MTPALAIILGVILAAAAAGFLAGRRIPMNLENWTVGGRKFGVVI
                     IWLLMAGEVYTTFTFLGASGWAYAKGAPTYYILIYGALAYVLSFFILPEIWKIGKKAG
                     LHTQPDFFVHVFQSRGLGILVAVIGILSILPYLQLQLAGLGLIVEAASDGAVSSTAAI
                     LLSFALTTAFVLTSGLRGSAWVSVIKDILMLAAVGIVGFGLPRIHFGSIGGMFRALMD
                     KAPGHLVFPGAAPGMGVPWVMSTVLLTAMGFYMWPQLFGTAFSAKSDRIIRRNAVIMP
                     LYQIPIVLVFFVGFTAFLILPGLKTGDLSLLMLIRRTYPPWFVGFVGAAGAVTAMVPS
                     SVLVLSAAALLSKNVYRAGFRPGASDAAVMRVSRWMVLAVATAALVFALVLPNALVNL
                     LLLGYDGVAQLFPGVVLGLFWKKVTRRGVTAGLAAGIAAAAFLVFGKHDPFCGLNAGF
                     VALLVNILTVTVVSLAGRRRPAPRAG"
     gene            55093..56262
                     /locus_tag="PKV64_01895"
     CDS             55093..56262
                     /locus_tag="PKV64_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPN15863.1"
                     /translation="MYEDIKNEAEALSGRMSEWRRAFHRRPETANNEHETSAALKAFF
                     ERLGLPVRRLAGTGLRADLDGLPGGRTVALRADMDALPLEEEGEKPYRSEIPGACHAC
                     GHDGHLAGLMGAAHILAARRDKIRGRVVFLCQPAEELPPGGAGPMIEAGALEGVDGIF
                     GIHLWQTLPTGKIGLVKGPVMAQADNFRITVKGRGGHGSMPQATADPIVAAGHLITAL
                     QGIVSRNVDPLKPAVLSFGTIRGGTVYNIIPNEVHLSGTVRAFEPAVQELMKKRLGEI
                     TEAVGRAGGVQAFLEYEEGYPPLVNPSASVDFAAQTIEALWGREVFAPFAPVMSGEDF
                     SRYLRKAPGAFLFLGIGDGMPYPHHHPGFDLDERSLPRAAALLAALAVSFLEQPE"
     gene            56259..56828
                     /locus_tag="PKV64_01900"
     CDS             56259..56828
                     /locus_tag="PKV64_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPN15864.1"
                     /translation="MSFGVLLALALAMAMDTFAVAIGVSLVMKGCTPRQTFRLAGSFG
                     SFQFLMPLLGWTVGRTVVDLVRGFDHWLAAGLLAVVAGKMIVEALEKKKDGKAAGPCP
                     DKTRGGHLLLLSLATSIDALAVGLSLAALNVSVVYPAAVIGLVTFVVSAVGTKIGPVL
                     GRWAGKSAELAGALVLLAIAAKILVEHLS"
     gene            complement(56880..>57777)
                     /locus_tag="PKV64_01905"
     CDS             complement(56880..>57777)
                     /locus_tag="PKV64_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15865.1"
                     /translation="LPADAYLCGTIPPDEAASLKETSLRVPFADVFSESQILIAPTEY
                     STTAPLKLAAKHYGFRAATMPGFGPEMIPALRVDYVQVARYVEAVKARLDKAVGADIL
                     FLVDGRVEARMHFDLRHRTAHSSTGRFPEIGTAGNLPSGEAYIVPYEGEGKAPSATAG
                     VLPVEIGGEVVYYKIEKNTAVSAEGGPTAQEESDYLKREPAYGNMAELGFGVLGKFGL
                     SPCGEILLDEKLGLHIAFGRSDHFGGRIGLKDFSTPAAVIHLDRIYLPEMQPRVAVVE
                     VVLSFARGRTEMIMKDGRYTIF"
BASE COUNT        10890 a        17634 c        18342 g        10911 t
ORIGIN      
        1 ggccaaggcc cggcggctga agctcgaggg cctcctggac atcgtcttca tcgattacat
       61 ccagctgatg cgggccgggg ggcgctttga aaaccgcaac caggaaatgt cgttcatttc
      121 ccgttcgctc aaggaattgg ccaaggagct ggccatcccc gtcgtcggca tctcccagct
      181 cagccgggcc ccggaaaagg cccgccgcga gcctcgcccc atgctttcgg accttcgcga
      241 gtcgggggcg atcgagcagg acgccgacgt cgtcattttc atctaccgtc cggagatgta
      301 cttcacacct gaagaaaagg cggttcaaac ggagctccgg ggaatcggcg aagtgatcat
      361 tgccaagcag cggaacggcc cgacggggca gttcgaactg gcctttcaga acaagttcgc
      421 caagttcgaa aacctcgaaa cgtcccatcg cggcccggag gagtgaggat tggccgcccc
      481 tccgcgttcg atcatcgtcc gtttcttcga gcagttgggg gagctgtccc tcctggccgg
      541 ccggaccttc cggagctttt ttcaaaagcc ctgggagggg aagatcttcc ttcagcaatt
      601 ggaggaaatc ggcctccgga cgcttcccgt cgtctccctg accgcggcct tcggcggcct
      661 cgtcttcggg ctgcaaagct attacggatt tcacaagtac atcggccagg ggtcggaggc
      721 ctacggcggg ccgatcatcg ccgtcgggct cgtccgagag ctcattccca ttctcgtcgg
      781 cctgatggtc gccggacggg tcggctcctc catggccgcc gagatcggaa cgatgcggat
      841 caccgagcag atcgacgccc tcttcacctt gggggcggac tcggtccgct acctggtcgc
      901 tccgcgcacg gcggccgctt tcctgatgct tccctgcctg accctgttcg gcgacctcat
      961 cggcatcctt tgcgccgaat tctacaatgt cgtcctgatg ggcgtgaaca agaccgtcta
     1021 ctggcgcaac acggtcacgt acttggacct ctgggacgtc ggctcggggc tggtcaaggc
     1081 ggccgtgttc ggagtcctga tcgccatcat cggctgctgg cagggcctgg aaaccgaggg
     1141 cggggccgag ggcgtcggcc gggcgacgac ccgggcggtg gtcatcgcct cgatcatcat
     1201 cctcatcagc aacttcttcc tgagcgagct tcttccttcc agcctatgat ccgattcaac
     1261 ggcgtccaca aatccttcgg cccgaagcgg gtgctgaccg gcctcgacct ggaaatccgg
     1321 accggggaaa cgctggccgt catcggccag agcggatcgg gcaagagcgt cctgctgaaa
     1381 atcctgatcg gcatcctcgc ccccgagcgg gggaaagtct ttgtcgacga tgtcgaagtg
     1441 accgccctgg gacgggacga gcgccaggcg acggcccgca agttcggcat gctcttccag
     1501 gcggccgccc ttttcgattc gctgaatgtc gccgagaacg tggccttcgg cctgaggcgc
     1561 cggatgcgcc tgagcgaaca ggagatcgcc ggcctcgtcg ccgacagcct ggccaaggtc
     1621 ggcctgcggg gaatcgagaa gttgatgccc cacgagcttt ccggcgggat gcgcaagcgc
     1681 gtcggactcg cccgggccat cgcctaccgg ccggagatca ttctgtacga cgagccgtcc
     1741 accggcctgg atccgatccg ggccgacgcg atcaacgatc tgatccttct cctgcagaag
     1801 gaaatgggcg tgacctcgat cgttatcacc cacgacatgg tcagcgccta caaagtcgcc
     1861 gaccggatcg ccatgttata cggcggaaaa atcatcgccg tcggtccgcc cgaagagatc
     1921 cgccattcgc cggatccggt ggtccagcag ttcctccagg ggcgggcgga gggaccgatc
     1981 aaggattttt aatggaggaa ggcatgagcc gaaaaacgcg agtcggcctt ttcgtcggag
     2041 ggacgctggc cgtcatggct gttttcatca tgttgctcgg cgacatcggg tacgtcttta
     2101 aaaagagggg atacaccctc aatgtcgacg tgccgacggc ggccggtctg gacgccaagg
     2161 cggtggtcaa gatggccggg gtcaagatcg gcttcgtccg cgacatccag ctctccggcg
     2221 tccgggcccg ggtcgtcatg aacatcgaca acggcgtgcg ggttcccgtc gactcgacgg
     2281 ccgccttctc cacgctcggg ctgcttggcg agaaatacgt cgagatcgat cccggcgaat
     2341 cggcggagtt ttgcgctgac aacgcctcca tcgccggctc ccagaaaccc ggattcgacc
     2401 aggtcggtga gatgctctcc tcggtcggcc gggagttgaa agccgtttcg gcctccttga
     2461 gcgacgtgct taacgaggag aaccgggcca agttcaagcg ggccttggag ggagcggcgg
     2521 gagcggcggc cgagctcgaa acgattctga ccgtcaaccg gtccgaattc gagcggctga
     2581 tccggggggc caacgagacg gtcgacggcg tcggccggac ggtggccgac gtctcggccg
     2641 acttgaaaaa atcgttggcc gtcctccagg agacgatcgc cgagaaccgc gaaccactca
     2701 aggccgactt ggaaaaaatc ggcgaggccg cggccaagct ccgggcgatc ctggaaaaaa
     2761 tcgagagggg cgagggcacg gccggaaaga tcatccagga gcccggcctt tatgacgaag
     2821 cggccgaggt cctctcctcg gccaggaaag ccgccggcct cctttcaggg gttaaaggat
     2881 acgctgattt ccagacgggg tattatggaa caagccgtct cgtccgggcg ggcctcgcgg
     2941 ccgggatccg gccggcgaac ggctcgtttg tcgaggcggg gatcgttcgg gatccctggg
     3001 ccgggcggtt tgtcttttcc ctccaggggg ggctccggct gggacggttc gccccgcggc
     3061 tgggattcat cgagaacgag ctcggcttcg gcctcgatta tctcggcggc gatctttggg
     3121 gattgagcct tgagggattc aatttcaacc gggaggaaag cccgcggttc cggttgacgg
     3181 ggaagatctt tcccacgcgg tcgctctact tggtcgccgg ggccgatgat ttcactctgg
     3241 ccgggcgccg ggagtttttc ttcggcctcg ggctggctct tcgatgaaga acaaatccgt
     3301 cttcgtctgc caagggtgcg ggacgaaatc gccgaaatgg atgggccgtt gcccggcctg
     3361 cggcgagtgg aacaccatgg tcgaggaagc ggaggtcgag gaggccccgc tggtcgagca
     3421 ggccttcgct ccggccgaac cgattcccta tcaggacgtc aagaacctgg ccaagccccg
     3481 ggctcgggtt gaaatcaagg aagtcgacgc ggtcttgggc ggcggggtgg tcctgggatc
     3541 cctggttttg atcgggggcg agcccggtat cggcaagtcg acgctgctgc ttcagatgtc
     3601 ccgggatttc gcggccggtc gaacgccggt cctctatgtc tccggcgagg aatcgctgga
     3661 ccagatcaag ctccggggcg agcgtttggg cgtcggcggc gggcctcttt ttcttttggc
     3721 cgaaacgaac ctggagcgga ttctggctca ggccgagaag cttcgccccg gcgtcctcgt
     3781 cctcgattcc gtccagaccg tcttttcctc caagctgtcc tccagccccg gaacgatcag
     3841 ccaggtccgc gaggttgcca accaggtgtt ccgattcgcc aagcgcaacg gcgttcccgc
     3901 ctttctcatc ggccacatca ccaaggaagg atcgctggcc gggcccaaat cgctggagca
     3961 catcgtcgat gtcgtccttc tgttcgaggg ggagcgcgac cacagccacc gggtgctccg
     4021 ggccctgaaa aaccggttcg ggccggtctc ggagctggcc atcttcgaga tgacggcggc
     4081 cggcctggaa gcgattcccg atccctcggc tttcttcctc agggaaaggc cgaagaacga
     4141 gtcggggagc gtggtggtcg cgaccatcaa ggggacgcgc cccctgctca tcgagatcca
     4201 atcgcttgtt tcgccgacgc tgtttgcctc caatccgcgc cggatgacgg tcggtctcga
     4261 ccactaccgg acgtcgatgc tcctggccgt catcgagcga aagctgggtt acacgttcgg
     4321 gacggaggat atttatttga acgcggccgg gggaatggag atcggcgagc cggcggccga
     4381 cttgggcgtc gtcctgggaa tcgtctcctg cctgatgaac cgacccgttc cggccgggct
     4441 ggcggttttc ggagaggtcg ggctcagcgg ggaaatccgg tcggtcggcc aggccgcggc
     4501 ccgggtccgg gaagccgcgg ccctgggatt cgggacgatc cttctgcccg agggaaacgt
     4561 cgccggcctg gccaaagaga aaatcgacgg ggtccggctg ctgggagccg ggacgctgag
     4621 gcgggcggtc gacctcgttt tctgatatga tagagcctcc ttgcgaggga tcttccactg
     4681 aaagaggatg cggtcatggg catttttctt tttcggattt tcttcgtcgc tgtgaccgcc
     4741 gcggccggat acttcgttcc gcccttcggg ctcgaccgga ttccggcggc cgccgtcgcc
     4801 gggtttctgg ccgcggcgac cctgtttctc gagtcgcgcg tccgccgggc gtccttcacg
     4861 gtcatttggg gctcggtcgt cggaaccctg gtcggcgtca tcctaggctg gatcctcggg
     4921 gcggcttacc aatccatgac ctccgatccg gggacggccg ccttcgtccg ggtcatgttt
     4981 ctccttcttt tgccggcggc cggcttcttg atcgggatcc ggaaatcgga atggctcgat
     5041 ccgtcgtatt tgttccgctt tttccgtgaa aaaaacaacg gccggatctt taaaatcctg
     5101 gacacaagcg tcatcatcga cggccggatc gccgagatct gcgaagccgg tttcctcgag
     5161 gggacgctgg tcgtgcccca gttcgtcctc aaggagctgc agatggtggc tgattccccg
     5221 gacgggctca agcggcagcg gggccgccgc ggcctggaca tcctcgatca tctgcaaaaa
     5281 tccaagacgg ccaacgtcat gatcaccgag atggattttc cggaaatccg cgaagtcgac
     5341 acgaagctga tcgaggtggc ctgccgcctc gagggcaaga tcgtgaccaa cgacgtcaac
     5401 ctgaacaagg tcgcccggct tcgcggcgtg gccgtcctca acatcaacga aatggccaac
     5461 gccatcaagc ccgtcgtgct ccccggcgaa tccatgatgg tctatctcat caaggaagga
     5521 aaggaaaagg accagggcgt ggcgtacctg gatgacggga cgatggtcgt cgtcgacaac
     5581 gcccgccgga tgatcggccg caacgtcgac atcatggtca cctccgtcct ccaaacgacg
     5641 gtgggcaaaa tgatcttcgg ccggtacaac gacgaggcga aatgaagagg acggccgccg
     5701 tgatcgtcgc cgccgggtcc ggggcgcggt tcggcgggcc caagcagttc gccctcctgg
     5761 gcggccggcc cctggtcgag tggagctgcg cggccttcca ggcccatccc cgcgtcgagg
     5821 acattatcct tgttctgccg gagacggccg cgggggcgga atatctccgg cgctttccca
     5881 agatccgggc cgtcgcggcc ggcggcccgc gccgccagga ttcggtcgcg gccggcgttt
     5941 cccggctgga tgagcggcgg acgggagtca tcctcgtcca cgacggggcc cgtcccctgg
     6001 tctcggccgg cctgatcgac cgggtgatcg aggccgcgga aaaacgagga gcggccgtgc
     6061 cggcggtccc ttgccgggat accctgaagg aaatcgacgg cggccgcgtc gtccggaccc
     6121 tcgaccgcgg acgcctggga cgcgttcaaa caccccaggg cttcgcctcg gctctcctcc
     6181 ggcgggccct ggccgcggcc tcggccgagg ggatttcggg caccgacgag gcggcgctcg
     6241 tcgagcggct gggggaagaa gtggccgttg tttccgggga tccggaaaac atcaagatca
     6301 cgacgccctt ggatttgatc gcggcggagg cgatccggca tgagaatcgg attgggatat
     6361 gacctccacc ggctgaccgc cggccggccg ctctatctcg gaggcctccg gatacctcac
     6421 tcccggggcc ttctcgggca ttcggacggc gacgtgatca tccatgccgt gatcgacgcc
     6481 ttgctcggag cgggcggcga gggcgatatc gggcggctgt tccccgatac cgatcccggc
     6541 ctgaaaaacg tccgaagcac ggaattgctc cgggacgtcg tctcgcgcct gcggcggaag
     6601 cggcttcgga tcgtccatgt cgatgccgtc gtcgtggccg agtcgccgaa gctgggtcct
     6661 tactttgaaa agatgaaaga cgtcctggcc cctctcctcg gccttccccg ggaccggctt
     6721 gggctgaagg ccaagaccaa cgagggtctc ggcgagatcg gccgcggccg ggccatcgcc
     6781 tgttgggcgg cggcttcgct gcggcccgtc tccgggcgga agccgaaaga gtaaagcccg
     6841 ttccgggaag aaacaccccc cgccggcgtc caagagggga ggaaaaacca tgagaaaaaa
     6901 cgttttcatt gtttgcgtcg ttttgttctc gagcctggcg gcgtcggcgg cgttcgacct
     6961 cggcggcagc tgggagttga cgacggtgac gccgaggggc gaacgggtgt cgaccgtcgt
     7021 cttcgtccag gagggcgaac gcctgaccgt cacggtcaaa cggccgggcc gggacggccg
     7081 cctggtggaa atgacgtccg aagggacgtt taagggagaa accatcgaat ggaaggaaac
     7141 ccggcggggt ccggagggac gggaaatgac cgttgcctat aaagggaccg tcgccgccga
     7201 cggcagcctg aaaggcacga tggagttcag cgggggccgc ggcttcgccg gcggggaagg
     7261 cgcgcctccg gtgttcgagt ggaaagccgt ccgcaaagcg tcgtgaagcc gggcgttccc
     7321 gtcggatcgc gccttccgga tgaataggtc cgccggatcc cgtttttgct ataatggggc
     7381 ggatttgcgc gccgccgccg tcttcccgga cggggaagga agacgcgggc ggaggatgcg
     7441 gccggtctat gcccccgggg aagaattccc cccgccttcg cgtctaatgg atgacggagg
     7501 caaacgagcg tgaacacgga tggacgaaaa gacgctggtc gagttgtccc ggaacggacg
     7561 gacggacgcc ttcgaaaagc tggtgcgcat gtatgaccgg aaaattttcg ccctggccta
     7621 cggcttcgtc cgggacaggg ccacggccga cgacctggcc caggagatct tcctcaaggc
     7681 ctaccaatcg ctgccgtcgt tccgcgtcga atcgggattc ggcacctgga tttaccggat
     7741 cgcggtcaat cacgtcaagg atcacctccg gaaagcttcg cgacgccgga ctgtttccct
     7801 cgacgatgtc ggcgacggcc cggtttccgt cgacgaagaa accggcctcc gtctccgcga
     7861 acgcgaggag gaagaacggc ggaaagaggt tttccgggtc atggaaacac tgccgccgaa
     7921 attccgggcg attctcaccc tccgggacgt ggaggggctt ccctatgagg aagtcggccg
     7981 gattctcggg atcgccccgg gaacggtcga ttcccgcctt ttccgcgccc ggagaatgct
     8041 tcgaaagaaa atggagccct atcttgaagc cgaaggagga aggccatgag ctgccgccgg
     8101 gtggagcgtt gtcttctcgc ggcctttgac cgggatcttt ccgcgccgga ccgccggtcg
     8161 atggagcggc acctggcgga gtgtccggct tgccggaaac gggccgaggc ccacgccgag
     8221 atccgccggc ggctttccgg ctcggctgtt cccgagccgc ttccccgctt ctgggaacgt
     8281 cttgaatccc ggatgacggc ctcaggcccc gccctgcccg cggcttgggc ccgcttgtgg
     8341 gccgaggcga tcccgatttc cctgtccttg atcgccgggt tcgtcctcgc caccttgttc
     8401 ttgttccctt cctcgcggtc ctccgacctg tccggctcgg aagccctgct cctgaccgag
     8461 ggaaatccga tcgcggaaac ccgggcgatc atcgaggaag gccgccggga cgtgcgcgac
     8521 ctgacggtgc tgttcgccgc cgatgaacag cccgccggaa agagatgaag gccatgaaac
     8581 aaacgcgttg gggaatcgtt ctttcactgg ggctggcgtt tgccgcgggc gcggcggccg
     8641 gcatgttcat cgaccgcgtc tttttctttc cccgggggca tcaccgcccc ggcccgtcgc
     8701 cggaccagtg ggagaaggac ctcggcctct ccggggagca aaaagcccgg atggaagaga
     8761 tcttcaagaa aaatgacgcg cggatcgacg ccctgcggtc cgatttctat aagcatttgg
     8821 gagagatccg ggccgagatc cggaaggaaa ttgataccgt cctgaccccg gaacaaaaag
     8881 ccaagcaaag cgccatgatg gaaaaattcc gcaagtcgcg gagcaaggat tccggttccc
     8941 ggccgtccgg cccgggccgg cccccggacg gcggcttctc gaaggagaac aaagatgaaa
     9001 aagaaagtgc tggcgggagc ggtgattcta gccgctatcg ccgtcctgat tatcggcatt
     9061 tcctttatta agaaaggcaa gaacaatgcg ctcccgtacc ggacggaagc cctgacccgg
     9121 ggcgacatcg agtccctggt cgtcacctct ggtaccgtca atcccgtcga catcgtcgaa
     9181 atcgggagcc aagtttcggg caagatcgtc cagcttcacg ccgatttcaa ttccgtcgtc
     9241 acggccggcc aagtcgtggc cgaaatcgac caggagattc tgaaggccaa agtccagcag
     9301 aacgaagcca gctacttgag ccgccaggcg tcccttgaac aggcgaaggt caacctcgaa
     9361 aacgccagga aaaaatggga gcggactcag gatttgttcg accgccagct cgtctctcac
     9421 gaggagcggg agaccgtcga ggccaattac gtcacggcca aggtgaatct ccaggtcgct
     9481 gaggcgagct tggcccagca gaaatcagcc ctcgattcca gccgggtcga cctgtcctat
     9541 gccatcatcc gctctcccat cgatggaacg gtcatcaccc ggaacgtcaa catcggtcag
     9601 acggtggccg ccggtttcca aacgcccatc ctctttaaag tggccagcga cctgaccaag
     9661 atgcaggttg aatgccccgt ggacgaggcc gacatcggcc gggtgaagga aggccaagcg
     9721 gtccggtttt cggtggacgc cttccaaggc gaagtcttca ccggcgagat ccagcaggtc
     9781 cgcaattcgc cgacgaccac taacaacgtc gtcacgtaca cgacgatcat caagaccagc
     9841 aatccggact tgaagctgaa gccggggatg accgcgaccg tctcgatcat caccggggaa
     9901 gcccggggaa cggtcaagat tccgcgggcc gccctgaaat tcacgccctc cctgtccgag
     9961 gaagagatga aaaaaatgtg ggaagaggcg agggccaagc gggaagctct gcggggatcc
    10021 gggccccggc ccgaaggctc cggcccgcgc ccgacggggc cggtcaccga agagatgcgc
    10081 aagcaattcg cggccgcccg cagccgcgac ggcttcggcc aggtctgggt tctcgaggaa
    10141 tctggtaaaa tccgtcccgt tcccgtccgc accggcatca cggataatac ctacgcggcc
    10201 ttgctgaacg gcgacttaag cgaagggatg aaagtgatcc tggggacggg aggtgccgcg
    10261 gcctcgaccc aggcgcaggg ttccgcgatg ggcggcatga tgatgatggg accgccccgc
    10321 cgctgagaac gaaggcaccg gcgatcatga acgcagacat cattcggctg gagaacatca
    10381 ccaagacgta tatcgtcggg gaaaccgagg tccgggctct ccgcggagtg agcaacatta
    10441 tcccgaccgg ggatttcgtg gcgatcatgg gggcctcggg ctcgggtaaa tcgaccctga
    10501 tgaacattat cggctgcctc gacaagccga ccagcggccg ctatttcctg gacggggagg
    10561 atatctccac cctgagccgc gacgctctgg ccggcatccg caaccgcaag atcggttttg
    10621 ttttccagtc cttcaacctt ctgcctagga cgaccgccct ggaaaacgtg gagctgcccc
    10681 tgatttacgc caatgtctcc cataaggaac ggctggcccg ggcccgggcg tcgctggcgg
    10741 ccgtgggcct caagggccgg gaagcccaca agaccaatca gctctccggc ggtgagcagc
    10801 agcgggtcgc catcgcccgg gcgctcctca acaagccccg gctgattttg gccgacgagc
    10861 cgacgggcaa cttggattca aaaacgagcg acgagatcat gaacgtcttc gagcggctta
    10921 accatgaaga cggcatcagc atcgtcatgg tgacccacga gccggacatc ggcgcccggg
    10981 ccaagcgccg cctccatatg aaggacggcc ttgtcgtccg ggaagactga ccaaggagca
    11041 cgggaatgaa cgtctttcgg atattgcgcg tttcgctccg ggccctggcc cggaacaaga
    11101 tgcggacgtt tctcacttgc ctgggcatca tcatcggcgt ggccgcggtc atcgccatgg
    11161 tcagcatcgg agccggggcc cgggcggccg ttgaatcgca gttcgcgtcc atggggacga
    11221 acctgatgtt cgtcaccggc aactggcgga tgatgggcgg cgcccggacc cagaccggcc
    11281 aattcaaccg cttgacagcc gaggacgcca aggccatcgg gcagctgccc tcggtcgagg
    11341 ccgtctcccc cagcgtctcg acccgggccc agctcattta tggaaacaac aattggaaca
    11401 cgatggtcca aggaaccgga gagagctatc ccctcgtccg caagtgggat atcgaggacg
    11461 gcgctttctt cgacgagagc atggtccgcg gagcggccaa ggtctgcgtc atcggggccg
    11521 acatcaagaa aaacctcttc gaggaggatg aggacgccgt cggcaagacc attcgcattt
    11581 cccgggtgcc ctttttgatt ctcggagttt tcaagtccaa gggggaatcg gggggcttcg
    11641 gcagccggga cgactttgtc tgcgttccgt atacgacggc cctcaaccgc ctctcccgct
    11701 cggagtatct ccagtccatc gacgtgtcgg cggtttccca ggcgaaaacg aaggaggccc
    11761 aggacgaaat caccgagctt ttgcgcatcc gccaccggat tcccgacggc gggaccgatg
    11821 atttcgtcgt ccggaacatg tccgaggtgg ccgagggcgc ggccgaagcg accgggatcc
    11881 tgacgatcct gctcggctcc atcgcctcca tctcgctgct cgtcggcggc atcgggatca
    11941 tgaacatcat gctggtttcg gtgaccgaac gcatccggga gatcggcatc cggatgtcca
    12001 tcggggcccg gccgcgggac atccttctcc agttcctggc ggaagcgatc gtcctgagcc
    12061 tcgccggcgg acttctgggg atcatcctgg ggatcggcgt ctccaaggtc cttaaataca
    12121 tccccgtttt cgcctccatc acgaccatgg tatcctggtc atcggtcctc ctggcgttcg
    12181 ccttttccgg atcggtcggc attttcttcg gattttatcc ggcccggaag gcgtccaagc
    12241 ttgacccgat cgaggccctg cggtacgaat aagggttgaa acgaccaaag gagaaagaag
    12301 atgcatatcc cgcgacggat cgccgttccc gcggccttga tcctcctcgc tgcgacggcg
    12361 ggggcccagg aaacgacccg atccctctct ctgaccctgg aggaatgcct cgtccgggcc
    12421 ctccggaaca acctctccat ccaggtggcc gtcttgacgc cccagggctc cgccttgtcc
    12481 gtccaaaagg cccgggaaaa gtttctcccc aatttcagct tcggcttgac gaagaggaac
    12541 tcgagcagtc cctcgttttc ctggctggaa tcccaggacg agaccttgac gaaatacggg
    12601 aactattccg cccagatcac cgaagtcatc cccttgggcg ggacggtgac cctcggcgtg
    12661 tcgtcggcca tgacggacac caaccagacc ggaacgacga ttaatccgcg gtacaacagc
    12721 cagctccagc tctccttctc ccagcccctg ttgcggaatt tcgggctgaa gatgacccag
    12781 atggagatcg tcatcgcccg gaacaacctg gatgtctccg agatccagct tcataaaacc
    12841 atccaagata cgatcttcag cgtcatccag tcctattgga atctcgtgta cagcatcgaa
    12901 aacctcaagg tccagcgcaa gtcgctcgag ctggcccggg atttcctggc gaaaaaccag
    12961 cggtcggtgg aaatcggcac gctggcgccc atggacatcc tggccgccga aagcgaagtc
    13021 gccacgaggg aagcgggcat tctagcggcc gaggcggcgg tcaaggccaa cgaagaccag
    13081 atgaaaaacc tcctcaattt caccccggaa gaggaacagg ggctccggga gatcgtcgcc
    13141 ctggattccc cccgtttcga ggaacggctg atcgaggtgg atgaagccct ggccgtggcc
    13201 atggagaaaa gaccggatct ccggatctcc cggatcaacc tcaaaaccca gaacctcaac
    13261 ctcgattatg cccgcaacca gctcctcccc gccctgagct tcaacgcgtc ctatgccagt
    13321 ccgggcgtct ccgggacgcg gctgatttac gacagcgagt atttcggcga catcatcgga
    13381 accatccccg gttaccggtc ggaatcctgg aatgacgtct tcggattcag atatgagaat
    13441 tggagcgtcg ccttgaccct ggacatcggc ttgaacacgg tcctgtcccg ggcggcttac
    13501 gcccaggccg agttggccac gcagacggcc ggcttgggtc tgaaaaacac cgagcgggcc
    13561 gccgtcctgg aaatccgcaa cgccgtccgg accgtccaga cggccttcaa gcaagtccag
    13621 gcctacaaga tcgcccgcga cttggccgaa aagaagctcg ccgccgagga ggagaagctt
    13681 cgcctcggct tgagcaccaa ttacacggtc cttcagtatc agcgcgattt gacgaccgcc
    13741 caagtccagg aattgaagtc ggtcatcgac tacaacatcg ccctggccga cttggatcgg
    13801 tcgatgggaa cgctcctgga gaatcgaaat atccggattg ccgacgcgct tggcggcgga
    13861 tcttattaag accgcgttcc cggcgctttg tgatcgagga gacgtcgcgc cggatgccgg
    13921 gcaggccggc catggcgagg cttcgcttcg acggccggat ggtcatccgt tatgaaggac
    13981 ggggcctggg cttccgggag gtcgccgagc ggccccagcg cgttcaggcc gtcccggtga
    14041 tccggccgaa gccgcccgag ttcatcccgc ctccaactcg ctggtggaag gtctatcggg
    14101 ggcccgcgga gaccgaagat ctcagatgaa gagaaaagcg gaaagattca cgttgccttg
    14161 agatcggccg cccgcgtctt gacccccgaa gggggcgaag tttatcatag ggccgtgaga
    14221 tcccgacgaa agacagagag gcggcggatt gttttttgcc tggccgccgt tgttttgcaa
    14281 tttctttttt cggcggccgc cgcccttccg ggaatcgtct tttgccatcg gcccggcggg
    14341 cggatcgccg cggaattttc cggtccgtcc ggcgcctgtc tctgcgacga gtgcgagcat
    14401 tgtctcgagc acctcgccgc ggcggccgcc ggtctttccc caaacggccc ggtcatcgat
    14461 gcctgccact gcgttcatga gccgtttcgg agtcaggccg accgagccgt cttgaaacgg
    14521 gacgaaggga gaacgcttcc cggcccggag gcgatccgcc ttcatgcgcc ggccctcttc
    14581 ccgctttcgg tccgcctccg gtccttcgct tcttctctcg atcctcggcc ggacgccggc
    14641 gtcttgcctc ccggcgccca ggccttacgc tgttagatcc ttcccccgcc ggaggacgcc
    14701 gaacgtcccc cggagcccgc cgtcggcggt caacgcggtt tatctccgca tccgccgtcc
    14761 ctccgtttcc ccggccgccc tggaggcggc tttcccgttg aggagtcagt ctcatgagcc
    14821 gtatctttcg ccctctcaaa aaccggcgtc gagccgccgt cctgtccctc ggccttgcct
    14881 tcgccgccgc cttggccgcc gcggccgcgg gcggccgcga cgaagccgag gagctgtccc
    14941 tggccggagc cgtcgagctc gccctggccg ggcatcccga aatcctcctg gtccgcgagg
    15001 aagccaaggc ggccgccgcc cgccgcaggc aggcggaagc ccgccccgat ccgcggctga
    15061 cgttcgggac ggccggcatc ccctggacgc tcaaagccgg agaagtcgaa accgaatatg
    15121 ccctcggcgt ggagcgggaa ttcgaatatc ccggactccg ggccgcccgg gtcgaaatcg
    15181 cccggagaga cgaggaagcc gcctccctgg agatcgagcg gaccgggctc ctgttggccg
    15241 cccgggtcaa gaaggcttat caccaggccg tcttcgccga ggaaacgcgg atcgccttgg
    15301 aatctctcgg cgcggtaatc gaccgtttga cggacgctct cttggtccgg tttgaaagcg
    15361 gggaagcggc ctatgccgac gtcctccggg cgaaggtcga gaaagcccgg ctgcaaaacc
    15421 ggctgattga agcccgaaga gacgagtcct ccgcccggac cgggcttctt ctctttctcg
    15481 gcttttcccc ggacaagccg gtccggctga cggatcgcct ggctgttccg cctctggagg
    15541 cttcgctcga tcgcgtcctg gccgaggctg ggaaacaccg tccctcgctc cggctcgccc
    15601 gattgcgccg gtcccgggcc gaggcggaag tccggatggc ttctctggcc gctaagcccg
    15661 gcttcgaggc cggtctgttc gtccccagca aaaatatcaa gggctggggt ttttcccttg
    15721 ggatcagcct gccgctttcc tcctcccgga cggggggcct tcaagcggaa gcggccgccg
    15781 cccgggaaaa aagcctgatc gaaaccgtcg ccgtggagag gcggctcgac attttggtga
    15841 gggcggctta ccgggaggcg cgggccgcgg aggagcaggt cgaggtcttc gaaaagaggc
    15901 tcctgggcga aatcgaggcg gagattgaaa acggattgac tcagtaccgc ctgggacgga
    15961 tcgaggcgta cgcgcttctc gatcttttcc gctcgctggc cgaggcgcgc ctcgagcatc
    16021 tccaggcccg gtttttgtat gccgtcgccc tggccggaat ggatgcggcc ggcgaggacg
    16081 gcgagtgagg ggaggaagat catgaatcga acgaaaatca cgacgatcct ggcgctggcc
    16141 gcggccatcg cggtttttcc ggcctgccgc cggagtctcc tgccggaaca cgccggacac
    16201 gacgcccaag agaacggccg cgttccggct ggaacggtga ttctctcgat cgcggccgcg
    16261 gctgacggag gaatctccgc ggcccgagtg gaaacggccg tcttggccgg ccggatctcc
    16321 gttccgggag agctcgaatt caatgcccgc cgcctggccg aggtctcggc ccgggtcgag
    16381 ggccggattg aaaaagcggc cgccgtcgcc ggagaccgcg tcgtcccggg ccagatcctg
    16441 gctgaaatct acagccggga attcttggcc gcgcaggcgg aagtccttca ggccgcggcc
    16501 cgggccgccc gccttcggga cggcccggaa ggtccggcgg ccgaggcctt cctccgggcg
    16561 gcccgccgca agctcctccc cctgggctgg ggcgacgagg agatcgacgc cttgcttgag
    16621 gccggagagc cggttccttt tctctccgtc cgggcgccgt tggccggaac ggtcatcgag
    16681 gcgccggcgt tggccggcgc gcatgtcgag ccgggcgatc cgctgttcaa gctggccgac
    16741 ccggcaagtc tctgggcgcg cgttcacgtt ttcgagaaag acctggccgc cgtccgcccg
    16801 ggatcttcgg ccgttctccg gacccaggct ttccccggcc gcgaattccc gggccggctc
    16861 gttctcctcg gaccggtcat ggatgaaaaa accaggaccg tggaggggcg gatcgaagtc
    16921 ggaaacgccg atggatgcct gagggcgggg atgtatgtcg aagccgcgct cgcctccggg
    16981 agagaaagaa cggccgtcgt cgttccggcc tcggccttgc aggagttcca atccctcccc
    17041 gtcgtcttcg tccgggaggg agagacgacg ttccggctcc ggccggtgga aatcggggac
    17101 cgggagggcg gcttggtcga gatcgtcgga ggcgtttccg ccggagaatg ggtcgtgacg
    17161 accggcggct tcctgctcaa atccgagctt ctgaaatcca gcctggcgga tgaacatgga
    17221 cacgattgaa cgactgatca gccgctcgct ccgcaacagg ctgctcgtcc tgatcggcgt
    17281 cgccttgctc gtcggcttcg gaatctggtc gttcctcaag attccgctcg acgcctttcc
    17341 ggacgtcact aacatccagg tcgaagtctt gtcctcggcc ccgggcttgt ctccgctgga
    17401 agtcgaaaaa ttcgtcacct ttccggtcga aacgaccctt caaggcctgc cccgcctgac
    17461 ccagctccgc tccgtttcca agttcgggct ttcggtgatc acggtcgtct tcgaggacgg
    17521 tgtcgacatc tatttcgccc gccagctcgt cctggagcgg ctgatcgaag cccgggagcg
    17581 cgttccccaa gggattgaaa tcaccatggg gccggtctcc acggccatgg gagaaatcta
    17641 ccagtacacg ctcgagggcc ccgtccccga aggggaggac gaggaagccc gggcggagcg
    17701 cctgaccgag cttcggacgg tccaggattg gatcctggcc cccctgctca agtccgttcc
    17761 cggcgtcagt gagatcaact cgttcggcgg gcacatccgc cagtatgagg tcaccgtcga
    17821 tcccgaccgc ctcctgaagt acggcctgac ggttgccgac gcggccgccg ccttggagag
    17881 gaacaatctc aacgtcggcg ggagcgtctt ggaggatgcc gccggacagg ccctggtccg
    17941 gggcgtcggc ctgctgcggt cggcgtccga catggagcgg atcgtcctca aatccgaggc
    18001 cgggacgccc gtctttctcg gcgacgtggc ggccgccgga aagagccggg ccgtgcggca
    18061 gggggcggcg gtcaaggacg gaaaggggga agccgtcggc ggcgtcgtca tgatgcttcg
    18121 cggagccaac ggccgcgaag tcgtccgggc ggtggaagcc cgggtggccg aagtcaacgc
    18181 cggcgcggtg ctcccgtccg gcctccagat cgttcctttc tacaagcgct cggacatcgt
    18241 ccaagccagc ctccacaccg tcagcgaggc cttgatcatc gggggaatct tcgttctcct
    18301 ggtcctgtac ctgttcctcc ggagcttccg gggggcgttc atcgtcatcc tggccctgcc
    18361 gctctcggtt ctgttcacgt tcatcgccat gcgcctgggc gggctatcgg ccaacctgat
    18421 gtcgctcggc ggcctggcca tcagcatcgg gatgatcatc gacgcgacga tcatccaggt
    18481 cgaaaacgtc cagaggcatc tttctgaaag cggacccgga aggaccaaga ccgagacggt
    18541 cctctcggcg gttctcgagg tccgcaagcc gagcattttc ggcgagctca tcatcgccct
    18601 gaccttcatc ccgatcttcg ccctccaggg catcgaagga aagatgttcg cccccctggc
    18661 cttcactcat gtgatcgccc tctgcgcgtc tctttgcctg tcgctcttgg tcatcccggc
    18721 gttctgccgt cttcttctca aaccccaggg cgaaaagcgg atcttcctgg ttgaagccgc
    18781 ccggaaagct tatcttcccc tcctccgctg gagcctggat cacaagaaaa cggtcttcgc
    18841 cgtttccctc ggcttgctgg ccgcgtcggc cgttctgatc ccgcgcttgg gaacggaatt
    18901 catgccgatc atggacgaag gggcgttcga catggatgtt caattcgctc ccgggatctc
    18961 gctgccccaa tccctggcca tgaatcggaa ggtcgaggag aagctcatct cctttccgga
    19021 aatcgagacc gtggtgggaa agaccggcca gaccggcatc gccctggaag cccggggcgt
    19081 ggaaaagacc ggatatgtcg gcattctcaa gccccgacgg caatggacct cggcccgctc
    19141 ccgcggcgag ctgacggaaa aaatgcggga ggccgtatcg accatccccg gaatggcctt
    19201 ttccttcagc cagccgatcg cctgccggat cgacgagctg gtcgccggca cccgggccca
    19261 gctcatcatc aaactcttcg gcgaggacct cgacactctg acggccaagg ccggcgagat
    19321 cgcggccgct ttgagacgac tgcggggggc ttcagatctg gttgtggaaa gcgtggccgg
    19381 gcagccgtat ctttccgtcc tgcccgaccg ggagaggatc gccaggtacg gactttcggt
    19441 cgaggatgtc cagacgctcg tcgaaacggc cgtcggcggc cgaacggtca cccggattta
    19501 cgaaggggag cgggcggtgg atgtccaact ccggtatgcg gagaaacggc gcggctcggc
    19561 cgaggcgatc ggcgctcttc ccttgcggac ggaacgggga gagatcgtcc ccctcagcca
    19621 ggcggcggcg atcgtccgct cggaagggcc ttcccagatc agccgcgaag cgggccggcg
    19681 gcggatcggg atcgaatgca acatcagcgg ccgggatctc ggcggattcg tggccgaagc
    19741 ccggcgggag gtcgcccgct cggtctctct tcctgcgggt tactttttat cgtggggcgg
    19801 ccagttcgaa aaccagcaaa gggcgatgcg ccggctgggc gtcattctcc cggtcaccgt
    19861 cgggctcatc cttctgctgc tctttttgac tttcaacgcc ctgcgtccgg ccctgctcgt
    19921 tttattcaat ctgccgttcg ccctgatcgg aggcgttctg tccctgtatc tgtccggcct
    19981 gtatctctcc gttccggcct cggtcggctt catcgccctc ttcggcatcg ccgtcctcaa
    20041 cggcatcgtt cttctctcgg ccgtcgccca gcggagggag gaggggcttg cggcgcggga
    20101 ggcgattctc cagggatgcc tcaaccggct ccggccggtc ctgatgacgg cgacgatcac
    20161 gatctgcagc ctcttgcctc ttcttttcgc ccaaggcccg ggatcggaaa tccagcggcc
    20221 gctggccgtc gtcgttgtcg gcggtctggc gacttcgacg ctgatgaccc ttctcgtgct
    20281 cccggctctc gaggcggctt tcggtgagga tcggatgcgg cggggaggca ggggccgccg
    20341 gccttgtccg gaaccggccg acgggttatg ataaaaccat gaaagacagg gccgggcggg
    20401 gagcgatctt ggtctcggct gtgcttcttt tgatgagctg cgcgccgccg ggccgagggg
    20461 tcattgtttt ttgcgcgggg gacagtctca cgaaggcggc ctatcccgat ttccttaggc
    20521 gggaattgaa ccgggacggg atcgcggccc gcgttctgaa ttacggccgg agcggctaca
    20581 cctcgggcga atacctgaaa ttcctcgtca agttcaggcc gaagctcatc gaggaaaaac
    20641 cggacgtcgt tcttcttcag ctcgggacga atgatgtccg gatcgaccat gacgccacgc
    20701 cgacggatgc gttcaaggaa aacatgaggc aaatcctggc ggtgttccga accttccgga
    20761 gccggtcggg gaaaatccct ttgatcctga tggccacgat tcccgatatt ccggagactc
    20821 cgcccaattt tgacgcctcc tcagcccgcc gggtccgggc ggagatcaac ccggccatcc
    20881 gggaaatcgc cgcggccgaa gggatcgcgg tcgtcgatca acacgccgtt tttctccgcc
    20941 ggcccgacct tctgcccgaa gtccatccgg tccgggaagg ctaccgcctg atggccgaag
    21001 tctggcggga ggccctccgc ccgcgctatt gacgcggggg gagctcggcc ttggaggaga
    21061 atgtcaccgg ggcgggatta cggggtcata atccggccgg ctcggaggag aagggatgct
    21121 tgttcaaaaa gggcgctatt cagctgcttt atgatgaggg ccgcgcgttt cgccggcggg
    21181 gcggccggcg tttgatcctt tcggccggtt tgtgataaaa taaatccaaa cgattttctg
    21241 aaatattccg acgccacgcc agggaatacg gagacttctc atgaacagac gcctgatccg
    21301 gccgcattta aatgaaatca aggagaacct gaaaggccag gagacgccgg tgccccacca
    21361 cggacggccg tcttccgccg gccactcagg ccatgcgtct tcgcgcggac cctatgcttc
    21421 ccagacgcag tcccagccga agaaaatgta cccgccgacg gacaccggcg ccgagaacta
    21481 ctactatctc aagcagatgg gaaaaaagac gccgatggcc gtcgtttttc aagacggcga
    21541 aatcctcgag ggctacgtcg aatggtacga ccgcaactgc atcaagctca accgaaacgg
    21601 cgcgccgaat ctgctcgtct ataagaacgc catcaagtac ttgtacaagc tcggcgaagg
    21661 caaggaatcc cccgaagagg acggcgagcc cgaaagaaaa tgacctttcc ccggatcggg
    21721 atctcgattg gagatcccgc agggatcggc ccggaagtga cgctccgggc gctgttcttt
    21781 tccggggcgc ttccccccgc tcggtacgtt cttttcgggc cgcgttcttt tttggaaaaa
    21841 gaagcggcgg ctttcggcct gtccacggag atcccttcct ggaaaacgga cgaatcatgc
    21901 gccggcgcgg gcgtttatct ctgggatctg ccgggaccgc gcgagccggt caagaaaggc
    21961 gagcccggcg cttccgcggg ccgggcgtcg tttgcctggt ttgaggaagc ggcggcggcc
    22021 gcccgccgcg gagagatgga cgctctcgtc accgcgcccg tggccaaggt cggctggaat
    22081 ttggccggaa tcccttggcg cggccatacc gaatacttgg aatccttttt tcccgacgcg
    22141 gtgatgagct tctggtcgga gaagctgacc gtcgctcttc tcagccatca tcttcccctc
    22201 cgggacgcgc tggcccgggt gaaccggccg gccctggccc gcctgttccg catcctgagg
    22261 gaatgtcctc cggcccgggc ggagggcccg gcttttgaaa tcctggtcgc cggactcaat
    22321 ccgcacgccg gcgagggggg gattctcgga acggaggaga gggacgtcat cgtcccggcc
    22381 gtcgccgaag cccgggcggc ggggaccccc gtgcggggtc cgttccctcc ggacgtcgtt
    22441 ttccgcctgg ccctcggccg tccggaaaag atcgtggccg cgctctacca tgaccagggg
    22501 ctcatccctt tcaagatgat cgcctttgaa acgggaatca acgtcaccct cggcctgccg
    22561 ttcatccgga cgtcgccgga tcacggaacc gccttcgaca tcgccggccg aacgccggcc
    22621 gatccggcca gcatgaccga ggcgctccgg cgggccgcgc gttcagcgtc cgcctgactt
    22681 gagggccgcc cggatctggc ggttgatgtc gcgctccttg agggcttcct ttttttcgtg
    22741 gattttcttg cccttgccga gggcgatttc gatcttaacg agacccttgt cgttgagcat
    22801 gacttgaagc gggatcaacg tcagcccgcg ttcctgaacc ttgccggtca agcgcttgat
    22861 ctcgcggcga tggaggagaa gcttgcggac gcggcggggg tcgtggttcc agcggttggc
    22921 ggcttcgtag ggatggatgt ggcagtcgac gaggacgact tctccgtcgc ggatgtcggc
    22981 gaagctctcc ttcaagctga tccgtccttc gcggacggat ttcacctcgc tgccctggag
    23041 cacgaggccg gcctcgaacg tctccaagat ctcgtagttg aaccgggcgg cccggttgac
    23101 ggcgaggact ttcatgtttc gacccggatc tggctgatgt gccgcttgtt catcttgacg
    23161 acgatgaagc ggcggccgtt gtggagaaga acgtccttct cccggggaat gcgtccgaat
    23221 tcctggagaa agaatccggc caaggtcgtg tattcggagc tttccggaag gccccagccc
    23281 agcgttttgt tgatgtcgtt gatcccggcc gagcccttga ccaggacagc cccgtcctcc
    23341 aggggcgcgt gccaatcctc ctcacggtcg tcgtactcgt cccggatttc tccgacgatc
    23401 tcctccatga tgtcttcgat cgtcaccaag ccttccatgt tcccgaattc atccacgagg
    23461 aaggccatct gaacgccctc gtcctgcatt tgaagaagga ctttttcgat cgaggcggac
    23521 tcagggagga aaaagggagg tcggagcacg gccccaagat caaagggccg gtcgtgagcc
    23581 aggtagccga tcaggtcctt ggtgtagatc aagccttcga tgtgatccat ccgtccgcgg
    23641 tagacgggga agcggctgaa gccctcggcc acgaccgttt tgagaatttg ctcccggctg
    23701 gaggaggcct cgatggcctt gatccggggc cggggggtca tgatttcctt gaccggccgg
    23761 cgggccaggt cgacgatttc cgtgatcatg gctttgcgat gctcgggcag gcccttgatc
    23821 ccggaatgaa ggagggcttt ggcctcttcc tcggccatgg cccgggagag ggcttcggat
    23881 gtcttggccc cggatcggaa cagccgccgg gagacgaagg tgaaaacggc gaccagggga
    23941 tagaacagga cgaggaaaag acggacgacg ggagcaaaga aaagggccag cttgtccgga
    24001 tggcgggcgg cgtagatctt cgggttgatt tcggaaaaaa gaagaaggat gagcgtcgtc
    24061 ccgcccgtcg ccgccaggac ggccgtgttc ctgtttggga tcaaggaggc ggccagggcc
    24121 gtggccagag aggccgcggc ggcattgacg agggtgtttc cgatgaggac ggccgagagg
    24181 aaatcttcaa gacgagaaag gatttttttt acccggccgg cccgcggcga gcccttcgtt
    24241 tccaggtaat caagggtgtg cggcttcacg gcgatgaagg ccgtttcgct tgaggaaaaa
    24301 aaggcgctca ggatgatgca gaggatgaac aagaggatca aggaaagggt catgaccgcc
    24361 ggtcagacgt caaggccgac tttaacgtct ttaaagcaag tggatagaat cgacgcgatt
    24421 ttcttttcga tctgggcggc cggcatggac gacacctggg cgatttccga aacgaccagt
    24481 tcccgggctt tttccatcat cttcttttcc cggaaggaca ggggcttgat caagctcagg
    24541 taatgaaggc tcttgagaac ggcgaccatg tccaggacgg tccccgattt catcaggccg
    24601 atgtgttcct tatagcgccc cttccaattc atggaaactt cgatttctcc gttcttcagg
    24661 aaatcgaaga tgtccttgac cgatccctcg gcgatcagct tgcggatgcc gatttcctgg
    24721 caggaggaaa aaggaaccat gaccagggtg ttgctggaca ggagacggag atgataaacg
    24781 gggaactttt cgccgttgta agcctgttcc tggacgtcga cgaccagacc caggccttga
    24841 ttgggataaa tcactttatc accaatcttg aaacattcca tgaggggtta attctagcat
    24901 tattaggaag tttcgtcaaa caaaaagaaa ggccggccga cggacttgtt ttccggtcga
    24961 cggccgacga acgaacgggg ggccgaacgg gaaaaagccg cttgaaaaca gaaccgattg
    25021 aaaaagaaaa aggtaggctt ggagcggagc cgcggcgctt cgttcaggag gattgccggc
    25081 ggaggcggtt gtagagtgtc gtgcgggaaa tgttcaagat ccgggccgtc tgggaaatat
    25141 tgtgatgggt cagggcgagc agcatcagga tgtattcgcc gacgagctcg tccagcgtcg
    25201 gaagggttga gggcggaacg gaacggatcg ggcccgtgga gaccagtgtt tccaggatac
    25261 ggttcatatt caacggcttc gggcctcgcg gaggaggcgg aagttgttgg aaacgacgta
    25321 ggagagagcc aggttggagg ccagggaagc gatccagccg aggggtttgc ttttcttgaa
    25381 aagcgccttg gttccgaagt aagtggccag ggtcaagccg cccttgaccg cggcgaacgc
    25441 cagatcgttc ttgacgaacg gcttcatgat gggattgcct tcggaaagat gggggaactt
    25501 gaggcattcc cgggtggaca cgtaatcggc gatgttcagg gcgaccatgg agatgagggt
    25561 ggcgtcgaag acggaatcgg cgaagcggga gcggcggagg gacggttcga taagggattg
    25621 agcggaggcg aggacgacga gccgtggatt gagggtgatc gaggagaaat cgggttcagg
    25681 ggcaggttcg gaagggaggg cgagggctga aagcgttccg gagggaagga ggggcgtttc
    25741 gttgagggtg gggaaagcca aggagacgcc ggaaaggagg ggaaaagtaa ttaggaaagc
    25801 aaggaccgtt tttttcatcc tgacgtctcc ttgtatcatt gaacataaag aaagcaaaaa
    25861 atagaccata tataaaaaca ttcataaaac attgaaaaaa aagaaaataa gatgaaaatg
    25921 aatgaaatga gctgtattta aataagaaca ataaataaac acaattatta aaagttagta
    25981 aaaacattga aaacaaataa aataagaacc taagctaaag aaaaaaagtg ttcaattagt
    26041 ggaattcttt gctcgattgt ttattgctat atctttcgct tcgaaaatcc tttaatattg
    26101 ccgaataatc gccttgcccg atgccggaga ggacgcgtcc ggcttgacat cgtcgccggg
    26161 gtcgattaag ataggcgcct tattcttgcg taaggtgtga tcatgaagcc ctcgtttaat
    26221 cctaataacc ggaagcgtaa aaaagcgcat ggatttctcg tgcggatgag ttcggccggc
    26281 ggccggaagg tcatcaagcg ccgccgggcg aaagggcgca agcgtctcgc ggctgaatga
    26341 gcgattctta cggacctcga gagaggatcc gaaaaaaatc ggatttttcg gcgctttacc
    26401 aaaggggccg gagctttcgg agccgatact tcacgatcgt ttatctttcc aacggcttgg
    26461 ggtatccgcg aatggccgcc gtctccagcc ggaaggtcgg caacgccgtc cggaggaatc
    26521 gggctcgccg gcgcgcccgg gagctttttc ggaggaacaa agagtcgttg aaaatgtccc
    26581 tggacatgct gttgatcacc cgagccgaat gcgccgacgc tccgtggccg cagctttgcg
    26641 agcaatacgc ggcttttctt gcctccctgg cccgggagaa atccctccga tgaaacgcct
    26701 tgccctggct ctcctccgcc tctacaaaag aacggtctcc cctttgctgg ggacgcactg
    26761 ccgatattat ccgacctgcg cggagtacgc ctgccaggcg atcgaaaaat acggcctgat
    26821 ccggggaacc gggctcgggc tcaaacgtct tctccggtgt cacccgttcc acgccggggg
    26881 actcgaccct gttccctgaa aaaggatcac tgatatggaa accagaagga tcattcttgc
    26941 gatcgtcctt tctctcggcg ttcttctgct ctggcaatat gtctttgtga aaaaagcgcc
    27001 ggaaacggcg ccccctcccc agccggtcgc cgcccaagcg gctgctcccg ctccggagac
    27061 ggaaacggcc ggccggcccg aagccgaaac agccccggcc gaggagcgcg cctcggacgc
    27121 cgcggaagcc ttgaatgcgg agaccgagga ggaaatcatc gtttccacct ctctttacac
    27181 cgcccgctgg accaacaagg gcggcgtcct cttgagctgg aagctccatc atcataagaa
    27241 cgagaaagac gaggctctcg agctcgtccc ggcggctgcg gaagcgtcgg gcgtccagcc
    27301 gctggccctt ctcgaggcgc tcgatccgtc ccgggcgacg atcgaggagc ttcgctcgac
    27361 ccccctgaac acggccttgt acgaggcgac gggcgggaac ctgagcttga aggacgggga
    27421 aaaaggcgtt ctccgttttc ggtacgccga cggacggggc cttgaggccg aaaaaacatt
    27481 cactttcacc ggcggacgct atgacgtcgg ggcggccgtg acggtccgcc gcggcggccg
    27541 gccggccgac gtccggatcg tctggggccc cggctgcggg aatccgtcgg aagccgaatt
    27601 gaaacagcgg ttcggcgcgg gagccggggc gagtttcctg gccggaacca aggtctaccg
    27661 ggtcgaggag aagaagtaca agccggaatc gagcgtcttc aacttcctca cgtggtcggc
    27721 ttatgacgac aattatttcg ccacgatctt ccttccggag accgcctccg gctcggcgtc
    27781 cttcctccgc ttcgaaaacg gcaagacccc cttctttttc ctggcggcct cggccccccg
    27841 gacgatcttc atcggtccca aggaaatggg agtcctgacc gccctgggat ccgagaccaa
    27901 gcgcgtcgtc cgctacggaa tgttcggatt catcaccgaa atcctctttg tctccctccg
    27961 ggcgatccac aaagtggttc ccaactgggg attcgccatc atcgttttga cgatcctcat
    28021 caagatcttg tttttcccct tgacgtacag cagctccaaa tcgatggccc gcatggccga
    28081 ccttcagccg aagatcaagg cgctgcgggc caagtacaag aaggccaaga ccgacatcga
    28141 gcagcgccgg cagatgaacg aggagatgat gaagctctac aaggagcacg gggtgaaccc
    28201 ggccggcggc tgcctgccga tgctcatcca gctgcccatc ttctggggat tcttccgcat
    28261 gctgacggtg gccatcgagc ttcgccacag tccctggatc ctatggatca aggatctctc
    28321 cgtccatgac cccacgtacg tcacgcccat cctcatgggt gtgacgcagt ttatcagcca
    28381 gaagatgacc ccgacgtcgg ccgaccccgc ccaagcccgg atgatgctga tcatgcccgt
    28441 ggtcatgacc ctctttttcc tgaacttcca aagcggtttg gtcctctact ggctgacgtc
    28501 caacgtcctt caaatcggcc aacaggccct gatgaaccgg atgatgcaga aaaaaaaggt
    28561 tgtctcccat ggaaaatcaa gaaaaaaatg acgccgtgac ggcggtcgag gaaatccccg
    28621 agttcaaggg gaagaacctc gaagacgcca tcctccacgc cgaacacaag ctgaaggttc
    28681 cgcggaccga gttcgaatac gaggtcgtca cggagaaaac gcgcctcttt ggtctcggca
    28741 aggaagtcgt catccgggcc cgggtcaagc ggttcgccaa gaacggcgac ctgaccgtgt
    28801 ttttggaccg cctgatgaac gtctttccgc tggatctgac gtacgaaatc aaaaccaagg
    28861 aagacatcag cttcgtcatc ttcggcggcg cggaccggca aattctcctc tggaaagacg
    28921 gggccttgct cctggcgatc cagcaccttc tcaacaagat ctccgaccgg aaggtccaag
    28981 tggattgcga attcttccga aagcgcaagg aaaagtcgct gcgggattac gccctcgacg
    29041 tggcccgccg ggtggccgat tccggccgaa acgagatcct ggacctgatg aatccgtacg
    29101 agcggcggat cgtccacatc gccgccaacc aagtccccgg gatcaccagc gaaagcctgg
    29161 gcgagggttt tttgaagcgg gtcaagatct atcccttgag caagcgcgcc cgctgacgcc
    29221 cgcggccggg cttttccgga acacgccgcc gtgtatgacc tctctctctc cgaaacgatc
    29281 gtcgccgtct ccacgcctcc gggctgcggc ggaatcggcg ttgtccgatt gagcggcccg
    29341 gcggccctta aaatcgcccg aacgcttttc cggccgaaaa aggaccggcg cggcggggaa
    29401 ccgggacggg ccgttttagg cgaggtcgtc gatccggccg accgccggcc cttcgacgaa
    29461 ggattccttc tccattttcg ggcgccccgc tccttcacgg gagaggacgt cgccgagctt
    29521 caccttcacg gaagcccggt catcctggag gaagtcgtcc ggctggccgt ccggcgggga
    29581 gcccggccgg ccttgcccgg ggagttcacc ctccgggcgt tcttgaacgg acggatcgat
    29641 ttccttcaag cggaagcggt cgatgacttg gtccgttcgg cttctctggc ccgggccaaa
    29701 tcggctttcc ggacggtcaa cggcgcgttg tcgagaaaag ccgcggccgt gcgccgggaa
    29761 atcatcggct gggtcgcccg gctggaagcc gacattgaat ttccggaaga gggacgtccc
    29821 gcggctaaaa aacactatat cgataaaatc gataaaatat atttagaagt cgatcgtctc
    29881 gtccgaagct acgaaatggg tcaggccctc ggccggggcg taacgatcgc cttggtgggc
    29941 cgggtgaatt ccggaaaatc gactctgttt aacgggttgc tcgaagagcc gagggcgatc
    30001 gtcactccgt ttccggggac cacccgcgat tttctccggg aaaccgtccg tatcaatgac
    30061 gcgtccttcc ttctcgtcga catggccggc ttgggccggg cgggatcgcc gatcgaacgc
    30121 gagggcatcc gccgcgggaa aaaaatcgcg gccgaggccg acggccttct tcttctcctg
    30181 gatggatccc ggcggctttc cgcttccgac gccgcgctca tcgagtccta tcggtctaga
    30241 aaaatcatcc tcgtcctgaa caaaacggat cttccggaga agacgactcc ggccgaggtg
    30301 acccgccgtt ttcccgatct tcccgtagcc gccgtgtccg ccctgagggg cgatggattg
    30361 gacgtcctcc gcaaaatgat gtacgacgtt ttcttcccgc tgattcccgg gcgagatatc
    30421 atcgtcttgc gggaaaggga taagcttctt ctggaggaaa tcgccgctcg ccttctccgg
    30481 gccaaggaga tgatcgcctc gaaccagccg atcgaattgg ccgccgagga agcccgcgaa
    30541 atcgtcaccc tcaccggccg gttgaccggg gagatccggc ccgacgatat cctggatgat
    30601 gtcttttccc gattctgcat cgggaaataa gtctcgcctt tctcttcacc gggcgctgtc
    30661 gggcggcggc cggccgctcc caagagcggg tcgaaccgcc gaattttcgg gagtcagggt
    30721 ataataccgg acaagatggc cgacgaatcc ttcgagatca tcgtttgcgg cgccggtcac
    30781 gccggctgcg aggcggccgc ggccgccgcc gccatggggt gttccgtctg cctgatcaca
    30841 acgcatttcg aaaccatcgc ccaaatgtcc tgcaatccgg ccgtcggcgg attggccaag
    30901 ggacacctcg tccgggaaat cgacgccttg ggcggattga tgggacttct ggccgatgca
    30961 accggcatcc atttccggct gctcaaccgg agccggggcg cggctgtcca agccccccgt
    31021 gtccagtgcg acaaatcccg ctaccgggag gcgatgaagg aacagttgga aaaaatcccc
    31081 ggcttgagcc tctatcaggg gatcgtcgtc gggctggata tcgtcgggtc ccggattcga
    31141 ggcgtcgtct gcggcgacgg ccggtcgatc gaggccgagg ccgtcatcct cacgcccggc
    31201 acgttcctca acggactcat ccatgtcggg caggcctcct atcccgccgg ccgggccaac
    31261 gagcctccat ccgtcgagct ggcggccgcg gttcggaagg cgggattgag aacattccgc
    31321 ttgaagacgg ggacgccgat gcggctggac gggaagacga tcgattggtc ggctttcgag
    31381 gctcagcccg gcgacgacga accggtcccg atgtcgctga ggacggagac gcggctcgaa
    31441 aacaaagtcg tctgttatct cggttatacc aacgagaaga ctcatgccgt gatccggcgc
    31501 gatctcgaca agtcgccgct ctacgccgga cggatcaagg ggctggggcc gcggtattgc
    31561 ccgtcgatcg aggataaggt cgtcaagttc gggcatcatc cccgccacca gttctttctc
    31621 gagccggaag ggctggcgac gtccgagatt tacgtcaacg gtctttcctc cagcctccct
    31681 tacgaaacgc agcgcgacat tctccggaca atccccggat taacggaggc ggtgattctc
    31741 cgtccggctt acgccgtcga atacgacgcc gtttccccga cccagctgaa gccgacgctg
    31801 gagtctcttc ccgtcgaggg attgtatctg gccggccaga tcaacggaac ttcggggtat
    31861 gaagaggcgg cggctcaagg cttggcggcc ggcgtcaacg cggtcttgaa gctccgagat
    31921 aaaaaaccgt tcgttctccg tcgtgaccag gcttacatcg gggttttgat caacgacctg
    31981 atttccaagg gagtggagga gccctaccgg ctgtttacgt cccgggcgga gtaccggctc
    32041 ggcctccgca tcgacaacgc ggattcgagg ctttcccctt atgggaggga gctcggcctg
    32101 ctttccgaaa gagatttcgt ccgctttcaa aaaaaggaaa agcggctgtc gtcgactcta
    32161 gcctttttgg aaaaaaccaa gatcacaccg gcggggaagg cccggatttc gctcaaggag
    32221 tatctccgcc ggcctgatat cgagtttgag aatgtgctag aatacgttcc gatgccggag
    32281 gccctttctc ctgaggaaac ccgccggatc gcggctgaag tcaaatacga gggctacctg
    32341 aggaaacaag aggtcgagat cgtccgcttg acgaaaatgg aggcgatgag aatcccggcg
    32401 ggattcaagc cttccggcgt ccccggcttg ggccgggaag ccgtggaaaa aatgaacaag
    32461 cacaagccga aaaccatcgg ggatatgaaa cgaattccgg ggctgacgcc gtcggatgtg
    32521 atgaacgtct ttattcacgt ctcggcgggg aatgttccac gtggaacatc gccgggaaag
    32581 ccgaccaggg aagatgaata aaatcattgc cgtcgcgaat caaaagggtg gggtggggaa
    32641 aacgacgacg tcgatcaatc tggccgccgc tttggcgctt aagaaatcga gggttctctt
    32701 gatcgatttg gatccccagg gcatggcttc ggccggcctg gggaaaccca aggccgactc
    32761 cgccgggatc taccaggcca tgatgaacgg ccgggacatc atggaatgca tctcgggcac
    32821 cgaaatggac aatttctatc tctgcccctg ttcaccggag ctgatgggag tggaagcgga
    32881 gatgttcaaa gccgaattcc gggagaataa acttaaaaac gccttggaaa aagcccggcc
    32941 ccggttcagc cacatcctca ttgattgtcc tccttccctg ggtttcctga cgattaatgc
    33001 cctcacggcg gcggactccg tcttgattcc ggtccaaacc gaattcttct gcatggaggg
    33061 aatccccgat ctccttcata ccctgaacga agtccggaca tattttaatc ccggcctaag
    33121 gatcgaggga gtcgttttga ccatggccga cgaccggacc aatctatctc ggcaggtcgc
    33181 cgacgaggtc cggaaatcgc tcaaagagct ggtctttcgg accgtcatcc cccgcagcat
    33241 ccggctggcc gaagcgccgt cctttggaaa gcccgtcatt ctatacgagc cgagatcgcg
    33301 gggggcggag gcgtatctat ccctggccca ggagattttg gaaaaatgaa aaaagctctc
    33361 ggcaaaggca tcaaggcctt catcccggag gaatacggca ttctcaagga agaatccttg
    33421 accgagatgg acatcgaccg gctgagtccg agcccgttcc agccgcggat gaaattcgac
    33481 gaacaggcga tcgaggagct ggcgcagtcg atccgcgagt cgggaatcct tcagcctatc
    33541 atcgccgttc cggccggcgg cggctataac atcatcatcg gcgagcgccg ctggcgggcc
    33601 gcccaaaaag tcggactccg caaggttccg gtcatcatcc ggaatatctc caaggaaaga
    33661 cagatggagg cctccatcat cgaaaacctc cagcgggaag atctcaatcc catcgaggtc
    33721 gcccgatatt acaaggggat ggtggaagtc ctggggtatt cgcaggagga ggtggccgag
    33781 aaggtgggca aggaccggac gtcggtctcc aacactctcc ggcttctcaa tcttccggag
    33841 gccgttcagt cgatgctgct tcaagaaaaa atcgccatgg ggcacgcccg ggccatcctg
    33901 gggatcgagg atcccaaggc ccaggtcgaa gccgcccggt gggccgccga ctcgggccgc
    33961 agcgtccggg aaaccgagga ctatgtctcc cggcgaagaa agaaaaaaac tccggtccgg
    34021 gcgaagctcg atccggatct tcaaaatctt caagaagact tgctccaaca cctgggggcc
    34081 aaggtcgtca tttccggaac gcctaaaaaa ggggtgatta aaatctctta tttttcgctc
    34141 ggcgagctga atcgcatcta tgaattgatc aaggggattc gctcatgaag gaaggaaagg
    34201 aaaaacgggt ctcgaaccgg gccgagctgt ccggattgta cgacaccgga accgaattcc
    34261 agggagtctt aaaattcgag ggatcgttcc ggatcgacgg ttatttcaag gggaccatcg
    34321 aatcggaaga caccctgatc atcggcgagg agggaaacgt ggaggcggag attcacgtcg
    34381 gacacgccgt cgtcaacggt gaattccacg gccggatccg gtgcgagaaa aaaatcgaag
    34441 tccacggccg agggcggatt ttcggaacga tcatcgctcc gaagatcatg atcgacgagt
    34501 cggcgtatct ccaggccgag tgccggacgg aggaggcccc cgtctcggcc gagcctgccg
    34561 cggaggacaa ggaaagcgaa tgaaacggaa agtcctggcc gtcatcccgg tccggttcgg
    34621 ctcgacgcgc tttcccggga agcctctcgc cctcattgcc ggaaagccga tgatccagcg
    34681 ggtgatcgaa ggcgtccggg tgtgccggtc gatcgaccgg gtgatcacgg ccaccgatga
    34741 caatcggatt tttgaaacgg tccgggctct cggagcggaa gccgtcatga catcgcccga
    34801 ccacgcctcc ggaacggagc gcgtcgccga ggtcgcggcc gctctcgatt atcccgtcgt
    34861 cctcaacgtc caaggagacg agccgcttgt cgatcgggcc gcgctcgacg gcttggccga
    34921 agccctccag gatcaccgcc tgccgatggc cagcgtgatg gcccgaatcg aggacatctc
    34981 ggcggcggcc gatcccaacc gcgtcaaggt cgtggtcgac gcctcggggc acgcccttta
    35041 tttttcccgg gcgctcatcc cggccggcgc gtccgacttt ttcttccagc acgtcggtct
    35101 ttacggttat cagcgggatt tcatcctccg tttcggccgc cttccccttt cgcgcctcga
    35161 acagatcgag cgcctggaac agcttcgcgt cttggagaac ggatttcgga tccggatgat
    35221 tgaaatcccc cgcccgacct tgagcgtcga tgtgcccgaa gatataatga gggtcgagaa
    35281 attcctcaag gaaagcggat cacggtgacc aaattcattt ttgtgaccgg tggagttctt
    35341 tccgccctgg ggaaggggat cgccgcggcc tcgatcggcc gtcttctcga aagccgtgga
    35401 tttaagctca ccatcatcaa gctcgatccg tatctcaacg tcgactcggg gacgatgagc
    35461 ccctaccagc acggcgaagt ctacgtcacc gacgacgggg ccgaaacaga cctggatctc
    35521 ggtcattatg aacgatttac gtcaacgcag acgacaaaat atcataattg gacggccggc
    35581 cggatctacg acatcattct aaaaaaagaa cggcggggcg aattcctggg caaaaccgtc
    35641 caggtcatcc cccatgtcac cgatgaaatc aagaacgccc tcaaatcggt cgccgagtcc
    35701 aacgacatcg tcatcgccga aatcggcggg accgtgggcg acatcgaaag cctgcccttt
    35761 ctcgaggcca tccggcaaat gcggctgtcc tgcggggccg accacacgct ttttgttcac
    35821 ttgacgtatg ttccttacgt ccagacgtcg ggcgagctga aaaccaagcc gacccagcat
    35881 tcggtccggg agctgcgggc gatcgggatt caaccggaca ttctgctctg ccggaccgat
    35941 cggagtctcg ggccggaaat gaaggcgaag atctccatgt tcacgaacgt cccggagaac
    36001 gcggtcatta ccgcccggga cgtcgagaac atttacgaaa tccccctcgt gttcgccgag
    36061 gaagggttgg acgcgatcat cctccgccag ttcggcttga cctcgcggac gcgccgtctt
    36121 tcccagtgga aaacgttcgt ccggcggatg aatcaaccga gggatgaagt cgacatcgcc
    36181 atcgtcggaa aatacgccgg ccttgaggat tcctacctca gcctcaagca ggccttgatc
    36241 catggggccg tggccgggga tctcaaggcc gtcattgaat ggattgaagc ggaaaatgtg
    36301 gagaaaggga agcttgaaaa gatcctcgga acgaaagacg ggatcctgat ccccggcggc
    36361 ttcggccagc ggggcatcga ggggaaaatc cgggcggtca ccttcgcccg ggaacacaac
    36421 gtcccgctgt tcggcatctg cctcgggatg cagtgcgcga cgatcgagtt cgcccggaac
    36481 atcgccggac tcaagggggc ccattccgcg gaattcgacg aagcgacccc ggcgaagatc
    36541 ttttttaaat ggcgcgagct gaaaggcgtg accgatctgg gcgggacgat gcgcctgggc
    36601 cggtacctct gccgcctgga gaaatcctcc ttggcatata aaatttatcg gaatgaagaa
    36661 atatgggagc gccatcgtca ccgtttcgag ttcaacccgg ccttcgaaaa gcctttgcgc
    36721 gaggccgggc ttcggttttc ggggaaaaat cccgattacg gcctggccga gatcgtcgag
    36781 cttccgggcc atccctggtt tctcggctgt caattccatc cggaattcaa gtcccgtccg
    36841 ttaaggcctc atccgctctt taaatcgttc atcggagcca gctatgccca tcgccaagaa
    36901 aagcgcgcgg cctgaggcgg cgcggtccgc cgttccgccg gccggattgt ttctcatcgc
    36961 cggtccctgc gtcatcgaaa gccgggcgaa aacgatcgcc cttgcccgga aactcgcggc
    37021 cgtcgccgcg cggctcgatt ttccccttct tttcaaggcc tcctacgaca aggccaaccg
    37081 gacgtcgctc cgatccttcc gcggtcccgg tcttcgggcg gggctggaga ttctccgggc
    37141 ggtcaaggaa acggccggga ttccggttct ctccgatgtc cacgagccgg cccaagccgt
    37201 tccggccgcg gaagtcctgg acgttcttca aatccccgcc ttcctctgcc gccagaccga
    37261 cctggtcctg gccgtcgccc ggaccggaaa accggtcaat gtcaagaaag ggcagttcct
    37321 cgcccccggc gacatgatgc atgtcgtcga gaagatcctc agcacgggca atgcccggat
    37381 catgctgacc gagcgcggga cgtgtttcgg ttaccacgac ctggtcgtcg atatgcgctc
    37441 gatcgcggac atgaagcggc tcggctttcc cgtcgtcatc gatgcctccc acggcgtcca
    37501 aaagccgggc ggcgcggggg gagcctcggg aggcgaacgg cggttcatcc cggccctggc
    37561 ccgggcggcc gtggccgccg gcgccgacgg agtgttcctc gaggttcacg acgatccggc
    37621 ccgggcccgg tccgacaagg acaacgcctg gccgatcgac gaagtggaag cgctgatgaa
    37681 cgtcctgctc cgagtctacg gagcgctggg agagccgaga tgacgaaaga agacatcatc
    37741 cgcgcgggcc gggacgtgct cgacgtcgaa gcccgggcgg tcgcccgtct ccgggagaac
    37801 atcggcgagc cgttcgtccg ggcggtgaat ctcctgtacc ggacgaaaac gagggtcatc
    37861 atcttaggca tgggcaagtc gggccttgtc ggccggaaaa tcgcggccac cctggccagc
    37921 tgcggcacgc cggcgatttt cgtccacccg gccgaagccg gccacggcga tctgggcatg
    37981 atcctggagg gagacgtcgt catcgccgtc tcgtacagcg gcgaaacgcg cgagatcatc
    38041 gacctcctcg gctatatcaa gcgtctcggg gtgaagctga ttttgatcac cggggcgccc
    38101 cgatcccata tcgcccgtta cagcgacatc gtcctggaag cccgggtcag caaggaagcc
    38161 gatcccgacg gattggtgcc gacggccagc tcaaccgcgg ccctggccct gggcgacgcc
    38221 ctggccatcg ctgtcatgaa acggaaaggg ttcaccgagc gcgatttcgc ctccgtccat
    38281 cccaaggggg ccctgggcaa gcgcttgctc cggatcgaga cgctgatgca caagggccgg
    38341 gccgttcccc gggtcgggcc gtccgccctg atgagcgagg ccatcgagga gatgaccgcc
    38401 aagaagctgg gcatgaccag cgtcgtcgac gaggccggcc gcctggccgg ggtcatcacc
    38461 gacggcgact tgaggcgcat gatccggaag cacggggcca cgctgctgtc ccggacggcc
    38521 ggggaatgca tgacgaccgg tccgctcacc gtgacccgga ccgatctggc ggccgaagcc
    38581 ttgaatgtca tggagcagaa ccggatcacg tcgctcgtgg ttctggccgc gggggggaaa
    38641 gtggaaggga tcatccacct ccacgacctc tggcggacgg agatgttctg atggggaaac
    38701 gcgggcgggc ccgccgcgtc cgcatgatcc tggcggacgt cgatgggacg ttgaccgacg
    38761 gctccctgac gccgtatccc gacggcgagg aaatcaagtc gttcaacacc aaggacgggc
    38821 tcggcgtttt gatttcccgg ctggccggct tgaaagtcgg gttcatcacc gggaaaaatt
    38881 cgaaagcctt ggaaggccgg gccgcgaggc tccggatcga cgagctccgc cagggagcga
    38941 tcgacaagga gccggtcttt cgggagatcc tggccaagca cggccttcgt cccgaggaga
    39001 tcgcctatat cggcgacgat ttgaacgacc tggcggtact ggaagcggcc ggtttttcgg
    39061 ccgctccggc cgacgccgtg gcggcggtcc gcaagagggt cgacttcgtc tgccgccggc
    39121 gcggaggcga aggcgctttc cgggagctgg tcgactacat cctgggagcg cagagaaaat
    39181 ggagcctggt caaagaccgg ttccgcgaga tatccgacca taaaatatga ccgccggaga
    39241 gcgggaagag gaggtccgca tgccgatcaa cgccaagatt ttccgcgagt acgatatccg
    39301 cggaatcgtc gacgtcgatc taaccccgga ggtcgtccag acgctcggcc gggcgatcgg
    39361 gacgtttttc aggtccaagg ggaaaaagga gaccgtggtg gggcgcgatt gccgcctgag
    39421 ctcgccggcc ttcgccgaag cgatggccgc cggcctgcgc gcggccggct gcgacgtcac
    39481 ggatctgggg acgattccga cgccgctgct gtatttcgcc ggctactaca agaatatgga
    39541 atcgggagtg atgatcaccg ggtcccacaa tccccccgag tacaacggat tcaagatgac
    39601 cgtcgggcag gacaccctct acggcgacga catccagcag atccaccgga tcattaaaaa
    39661 cggcgcttat gccccggccg ggaagggatc ttatcgcttg tacgacatca agcccgaata
    39721 cgaagcctat gttctcggtt tgttcaagct ggcccggccg ctcaaggtcg tcctggacgc
    39781 cgggaacgga acggccgggg tcgtggctgc ccccttgttc cgcaagctcg gctgcaaagt
    39841 ggacgagctc tactgcgaca tggacgggcg ttttccgaat caccatcccg acccgaccct
    39901 gccggaggcc atgcaatcgc tcgtgtcccg ggtgaaggcc acgaaagccg aactcggcct
    39961 ggcgtacgac ggggacggag accggatcgg cgtcgttgac gacgaggggc acttgatctg
    40021 gggcgaccag ctgttgatct ttttcgcccg ggacattctg aaggctttcc ccggggcggt
    40081 gatcatttcc gaggtcaagg cgaccaaggt cctgtatgac gaaatcaccc ggctcggcgg
    40141 caaggcggtg atgtggaaag ccggccattc cctcatcaag aagaagatca aggacgagga
    40201 tgccaagctc gccggggaga tgagcggcca tatgtttttc gccgaccggt ggttcggctt
    40261 cgacgacgcc atctatgcct ccctccgggt ggccgagctg gtcgcccgtt ccccccagcg
    40321 gctttcggaa atgctggcgg ccctgccgaa gacatacagc accccggaaa tccgcgtgta
    40381 cgcctccgag gaagtcaagt tcaagatcgt cgaggaggtc cgccgcgagc tctcgggcaa
    40441 atacccggtg atcgacatcg acggcgtccg ggcgatcatg cccaagggct ggggcctggt
    40501 ccgggcctcg aacacccagg ccgtgctggt cctgcggttc gaggcggaaa ccgaggagga
    40561 cctggaggcc attcagcggg aaatcaaggc ttccgtcgag cgggcgatcc acaagctcga
    40621 acggccgtca tgaaatccgc cgtcgatctt cacgccgtca tcatggccgg ggggagcggc
    40681 acccgctttt ggccgctcag ccggaagcgc cggcccaagc aattccttcc catcgtctcc
    40741 gaaaagacga tgatcgagga aacgtaccgg cggatccgcc ggatcgtccc cggccggcgc
    40801 gtctttacga tcgccgatgc tcctcagtcg cggagcatcc gccggctgct gccggccctg
    40861 ccggcggcca acgtcctgat cgagccgcgc gcccggaaca cggccgcctc gctcatcctg
    40921 gccacggccc acgtgcaccg gcggaatccc gaggcggtcg tcgccgtcct cgcctccgat
    40981 catttgatcc tcgacgagcc tttgtttctc aagaagctcg aggccgcggc cgaggcggct
    41041 ttccgaaccg aggggctcgt gaccttcggc gttcagccga cctatccggc cacgggatac
    41101 ggatatatcc aattcgaccg ggccggcggg cgggacttcg ccggcgagcc gttctacccc
    41161 gttcgggcct tccgggaaaa accgacgcgc gaggcggccg ccgccttcct ggaatccggc
    41221 gattacgcct ggaactcggg catgttcctc tggcgggcat ccgttttcgc ccgggccttg
    41281 gagagggatt ccccggcctt ctttcccttt tggcgaagaa cgcgggaagc cctcgacaag
    41341 aaatcgtcca aaaccctgat ccggatcttc gaggaaatcc cggcgctctc catcgactac
    41401 gccttgatgg aaaaagccaa gggaacgctc gtctgcgccg gcgatttcgg ctggtcggac
    41461 gtcggcgcct ggtcggccct gggagagatc tggccccggg acgatcaggg gaacgcccgg
    41521 cgcggcgacg tggtcgccgt cgagtccagg ggcaacatca tctgcaatcc gggaaagctg
    41581 acggccttga tcggggtgga gaacacggtg atcgtccagg ctggcgacgt tctcctggtc
    41641 tgccgcaagg accgggacca ggacgtgaag aaagtcctcg atcttttacg aggcggcggg
    41701 aaaacgaaac atctttgatc tccctgcccc gaaaggggga gccgaagctc cccctttcac
    41761 gcttccttgg cgcctaatgg caggagagaa ttttcgaatc gatggagaag cgagcctcta
    41821 gcggttcgtc cgccgcgatc gccgccaagc cgaagagcag cgttttttcc atgacttccg
    41881 cggccagggc cgcttcgccg gacttgagga atgaggatcc ggcgagaaag gaagcatatt
    41941 ctgttttcca ctccgccagg ctcgcggcgg aaacggcgga ccaagactgg gggaacttca
    42001 tattcaacgg cttcgggcct cgcggaggag gcggaagttg ttggaaacga cgtaggagag
    42061 agccaggttg gaggccaggg aagcgatcca gccgaggggt ttgcttttct tgaaaagcgc
    42121 cttggttccg aagtaagtgg ccagggtcaa gccgcccttg accgcggcga acgccagatc
    42181 gttcttgacg aacggcttca tgatgggatt gccttcggaa agatggggga acttgaggca
    42241 ttcccgggtg gacacgtaat cggcgatgtt cagggcgacc atggagatga gggtggcgtc
    42301 gaagacggaa tcggcgaagc gggagcggcg gagggacggt tcgataaggg attgagcgga
    42361 ggcgaggacg acgagccgtg gattgagggt gatcgaggag aaatcgggtt caggggcagg
    42421 ttcggaaggg agggcgaggg ctgaaagcgt tccggaggga aggaggggcg tttcgttgag
    42481 ggtggggaaa gccaaggaga cgccggaaag gaggggaaaa gtaattagga aagcaaggac
    42541 cgtttttttc atcctgacgt ctccttgtat ccacatatct aattaatcag caataaatag
    42601 accaacataa tcaatgtata taaatgcatg ggataattag aaataaattg ctagaagata
    42661 agtttgctga ttattatatc gtttaaataa tgaacaataa tgaatgaaat gtgtgtatct
    42721 cgttattatt tatataatta caaaaatgtc gaccattcag ggaataatgt tcaaatattg
    42781 ttcattttta tcccggtttt cgaacttttc tcgatgggaa tgatttggat ccgcgttgtt
    42841 cgggttctga atgacgggaa gccggaggag gggagcgggt gaagccgttg aaaagaggcg
    42901 gcggaatttt ttttcggcat cgttttcttc ccaggcttga gataatcatg gggaaggcga
    42961 ggaaaagagc ccgatcaata aaaaagaagc gggcaggccg gtcagcgccg attaaacggc
    43021 tgggaaaaaa ggcgcgggga gtttgatctg gaaggaaaac cccggcgaac cttctcccgg
    43081 cccaggcttt gtaagtttcc ccggaaagag gcgggcgttt tttttagtcg aatcgtccta
    43141 tataattcaa tccggaacga aaccagggaa atcatgaaat cagagtcgaa acggagcctt
    43201 cttcctttta ctcggagcgt gtgcggcctt gtccgccgga ttccacgggg aaaagtcatc
    43261 tcttacgggc ggctggcggc tctggccggg gacggccggc aggcccgcca ggtttcctgg
    43321 atccttcact cgacttcgga aaacgaaggc ctcccctggc accgggtgat caattccaaa
    43381 ggccgtgttt ctcttcctcc cgaacgcggc gggaacgaac aagcccgccg gctccgtcgg
    43441 gaaggcgtca agatcggccc cggaggaagc atcgacctcg ataagtacgg ctggcggatg
    43501 cgttggattt ccggacggaa gccgccgatt tccgagcgcg atctcgaacg gttttagccg
    43561 cctcggtttt acggattttg aaatcggaca aaggaaatcg ttccctcgag aggagcgatg
    43621 aacatctctt tcccagagcg caggttttgt cggaggcgtt ttcccggaag tcccgggccc
    43681 gtcgaggctt tcggcccccc ccggggccgg ctcgttcatc cgtcgcccag gccgaagccg
    43741 gcttaaggct tggccgcttt ttccctccgg agcttttccc tcgtctcgac aaacatcggc
    43801 tcggcgtagg tcgaatccca ggtccgctcg gcgaagcttc tcttttcgat aagcttgagg
    43861 aatccctcga agtcccggaa ataggagccg tagatgtgaa agctgtcggc cacatccacg
    43921 tagcgtccaa ggcggatttc ctttccggac cgttcggcga tccggccggc gatcgatttt
    43981 tgaagctcgg ttaaggcgaa cacgttcata taagcggcct tgaagccgtc gcgcgatcgc
    44041 caatgcgtgt tcatgttgag gacgaagctc ccgccgtcgc cgggaagcag ccggcaccag
    44101 acccgctgaa ggcagggagg atcgttcgtc ggcggatcca gggcgacgtt ccaggtgatc
    44161 gcctgggccc ggcgcgaatg gccggaacgg ctgagctttt cgacgaggga ttcgatctgg
    44221 tcgatcaccg ccccctcggt ctcataggcg aaaagccgct tgtggtacgt gtaggtccac
    44281 ttgccttctt ccggggcgat ccaatgatcg tggatgccgt cgacgacctc ctggcgatag
    44341 atttccagat cctccaggcc gccggggaac gcccgatgga tgcggggctc ggcgaacggc
    44401 tcctcgacca cccacatcat cgtgcagtcg cggctcggcg gatcgccggg cttgtcgtat
    44461 tccgttcgga cggcggcgcc gccgcgccag cacgccaaaa ccgcattttc ccaagcctcc
    44521 ggaagagttt ttccggtgac gaacagggcc ggaatgtcgc cgttcattaa ggcctcctcg
    44581 tttcggtctt gtatcacagc cgttccggag aatcaatcgc ccgaatcccg tcatcaaaaa
    44641 agtcgccgaa ggcggatata tcttgccgtt taaaaatctt gccgaagccg agaatcgttg
    44701 agagatcata aattatggga gtaacgcgcg gcccggcgag cgtccgccgc ccggaagcgg
    44761 cgggcgattg cgacgattct ccggccggag cgtctaccct gtggagaaag cgggtgtttg
    44821 ggagcggccc tctctccgaa aaagaagcgg agccatgaac gacgatttcc gccatccccc
    44881 cggccgcccc tccccccggg ccaagaatcc gcccggtccg gcgtccgccg ggcaccgaag
    44941 ccggctccgt gaaaaatttc tgaagggagg gttgtccggt tttctggatt acgaggtcat
    45001 cgagctcctg ctgacgctgg gaacgccccg ccgcgactgc cgcgaatccg cccggctggc
    45061 cttgcgggaa tttcggacgc ttcggggcgt gctcgaggcc gaaacgcacg agttgcggcg
    45121 gatcccgggc atcggctcgg ccaatgtctt cggcatccgg ctcattcagg aggtggcccg
    45181 ccgtttcctg aaagaccgga tgctccgccg cccctactgc cgttccgccc gggaggtgtt
    45241 cgaatacctt tatcatacga tgcgcgacct gaaaaaggag aatttcaaag ttctttttct
    45301 ggatgccaag aaccaaattc tcgaggagaa gacgctcttt gagggtaccg tggactccag
    45361 cgccgtctac ccccgggaaa tcatgaagga ggcccttcgc ttcaacgcgg ccgccttgat
    45421 tttcgcccat aatcatccct cgggcgatcc cgagccgtct ttttgcgacc gggaaatcac
    45481 caaggagctg gttttcgccg cccgggtcat ggggcttaag gtccttgacc acctcatcat
    45541 cggcaacaat cgctatttca gcttcgccga ccacggcttg atctccgatt atgatctcct
    45601 cttccactcg gtccaggcga aaaccggcgc tggaggctga ccccttcatt tcgcccgcga
    45661 ggtggattaa actggcaggc aggagggcct tttcggcccg agcagagacg acgcggagga
    45721 attgcgatga aaccaatcgt tgaaattgcg gccaagctgg gccttgatcc ggggaatctg
    45781 gttcaattcg gaacctacaa agccaaggtg gatccggcgg ccgggccgcc cggccggccg
    45841 gcggggaagc ttatcctcgt cacggccatg acgcccacgc cggccggaga gggcaagacg
    45901 acgacggcca ttggcttggt cgatgccctc acgcgggccg ggaaaaaagc cctcgcggcc
    45961 ctgcgcgagc cgtccttggg gcctgtcttc gggatgaagg gcggggccac cggcggggga
    46021 aaagcccggg tcgtccccgg cgaagacatc aaccttcatt tcaccgggga ccttcatgcc
    46081 gtctcctccg ctcacaacct gctggcggcc ctggtcgaga accgattgca tttcgacggg
    46141 ccggcgggac tcctggacgg ccgcaatgtt tattggaagc gcgttgtgga catgaacgac
    46201 cgggctctcc ggcaaatcat tatcggcctg gaggacacgt acggcggaat ggcccgggaa
    46261 tccggcttcg atatcaccgc cgcctcggaa gtgatggccg ttttctgctt gtcgcgggac
    46321 ttggccgatc tcaaggcccg gctggggcgg atcgttgtcg gctgcggcct cgatctgaaa
    46381 ccggtgacgg ccgcggctct gaaggctccc ggggccatgg ccgctctgct gcgggatgcc
    46441 ctgcggccga atcttgtcca gacgctcgag gaaacaccgg tctttgtcca cggcgggccg
    46501 ttcgccaaca tcgcccatgg ggccaactcg gtgatcgcca ccgggctggc cctccggctg
    46561 gcggattatg tcgtcactga atgcggattc ggctccgatc tcggagcgga gaagttcttc
    46621 gatatcgtgg tccgctccgg agccgtcccc ccgccttcgg ccgtggtcat cgtcgccacc
    46681 ctgcgttcgc ttaagtatca cggcggagtg aagcttgacg cccttcttca agaaaacatc
    46741 gcggccatgg ataacgggta tgccaacctt caagctcatg tcgatatcgt tcgccaatac
    46801 ggcttgcccg tggtcgtggc cctgaatcgg ttcccggcgg acagcccggc cgagatcgcc
    46861 gccttcaccg cccgggccgc cgccgaccgg ctgccgtacg ccctcagcga cgtgttcggc
    46921 gccggcggtc cgggcggcct ggatctcgcg gcaaaggtcg tggaggccgc gtcctccgac
    46981 gccggcgagt tcaggtttct ctatcccttg gaggccttga ttccggagaa gctggaggcg
    47041 atcgcccggc ggatttacgg ggccgaccgc gtcgttctgg aatccccggc ccggaaaaaa
    47101 ctgaaacggt tcgaggacaa cggattctcc gggctcccgg tctgcgtggc caagacgcag
    47161 tattcgctgt cggacaaccc caagctcctg ggccggccga acggcttccc tctgaccgtg
    47221 accgatctct cgatcagcgc cggggccgga ttcgtggtcg ctctctgcgg ggacatcatg
    47281 accatgccgg ggatgccgaa aagccccgcg gccgagcgga tcgatgtttc cgacgacggc
    47341 gttgtctcgg gcattctcgg ctgagcgggt tttactcccg gcggcgaatg tgataaaagg
    47401 aaacgagaaa aaagaggcgg ttcgagcgtc ctttaggatg tgcggcccgg tccggaggat
    47461 cccatgaaag acgaagagca gagcccgcgc gacgtgtccc ggggcaaggc catcgaagcg
    47521 gctgtttccc aaatcgaaaa gcaattcggc aagggttcgg tcatgaagct cggccaacgc
    47581 gacgcggccc agagaatcga ggccatcccg acgggatcca tcgccttcga cctggccctc
    47641 ggcatcggcg gatttccccg cggacgggtg gtcgaggtgt tcggcccgga agccacgggc
    47701 aaaacgaccc tggccctcca tgtcatcgcc gaggcccagc gccgcggcgg ccaggcggcc
    47761 ttcatcgatg ccgagcacgc cctggatccg acctacgccg gaaaagtcgg cgtcgacatc
    47821 gacaacctcc tcatctccca gcccgattac ggcgagcagg gcttggaaat cgccgaggtt
    47881 ctggtccgca gcggggccgt ggacgtcgtc gtcgtcgatt cggtcgcggc cctcgttccc
    47941 aaggccgagc tggaaggcga gatgggcgac gcccatatgg ggcttcaagc ccggctgatg
    48001 agccaggctc tccggaagct gacggccatc gtcgcccggt cgaagacctg cttcatcttc
    48061 atcaaccaga tccgggaaaa gatcggcttt ttcctgggga atccggagac cacgaccggc
    48121 gggcgggctc tcaagttcta tgcctccctc cgggtcgacg tccggcggat gacggccatc
    48181 aaggaagggg accaggtcgt cggcaaccgg gtcaaggtca agatcgtcaa gaacaagatg
    48241 gccccgccct tccgggacgc cgaattcgac atcatctacg gcgagggcat ctcgcgggag
    48301 ggcgacctcg tcgacatggg ggccaacgtc ggcttggtcg agaagtcggg gacctggtat
    48361 tcctacaagg gcgagcggat cggccagggc cgggagaacg tcaagaagct ctttaaggaa
    48421 aacaccgccc tggcggccga attggacgcc gatatccgcc ggaaagtcgg tctcggccag
    48481 ccggaggcgg aggaaaaagc ccccgtcgag cagaagccgg cccgggctcc ggaaaagacg
    48541 tccgaacggc gaagccgctg agccggctct tccccggaaa aaggagggga ggcggaacgg
    48601 cgggaggagg gagcggatgg cccggaatgc gtcttcgcgt tccaagaaaa gcggtctcgg
    48661 tcccggaagc ctcgtccatg tcggccggcg gaaaatcgac cggccgcgca tcaccctgat
    48721 cgattacgac aacggacatt tcctcgagaa ggaagtcgcg gccctcgagg agtgtttgcc
    48781 tttcaacctg acttccaccg tgacgtggat caacgtcgac ggcgtccacg atccggacgt
    48841 cgtcgaaaaa ttgggacagg ctttcggcct ccaccccttg attttggaag acatcatgac
    48901 gacgtcccag cgccccaagc tggacgacct cgaaacctcg ttctatttcg tcgcccggat
    48961 gatcgagctc ggcggccaag gccgggagct cgtcaccgaa cagctgagcc tggtgttcgg
    49021 ccggaatttc gtcctgacgt tccaggaggc tccgggcgac atgttcgatc cggttcgcga
    49081 acggatccga aacggcaagg gccgcatccg gaagctcggc ccggattact tggcctacgc
    49141 cctgctggat gccgtcgtcg atcattattt cgtcgttttg gaaaccctcg gcgaccggat
    49201 cgatacgctc gaggaggaac tcgttcgcga tccccgggaa gagacgctcc acgccatcca
    49261 cgctctcaag agagagatgt tgatcttccg caaggcggtc tggccgctgc gggaggtcgt
    49321 cgccgggatc gaacggacgg aaacgccgct gatccagccc tcgacgagca tcttcttccg
    49381 cgatgtctat gatcacatca tccaggtgat cgacaacgtc gagacatacc gggacatgct
    49441 ggccgggatg ctggagacgt acttgtcgag catgtcgaac cggatgaacc aggtcatgaa
    49501 gatcttgacg atcatctcga cgatcttcat ccccctgacg ttcctcgtcg gcgtctacgg
    49561 gatgaatttc gagcatatgc ccgagctcaa gtggcgtttc ggctatttcc tcatctgggg
    49621 cgtcatgatc atcctgggcc ttggcctcgt ccggatcttc aaaaggaaaa aatggttcta
    49681 ggccgccgcc ggccggagcg aaggagcgtc cgatgaaagt aaaacagatc gtcattctcg
    49741 tcctggtcgg cctcgtgatc gtcctgatga tccaaaattc cgtgctcgtc aagttccacc
    49801 tgctgttctg gcccgtttac gcgccgctgt tcttcctcgt tttgggcatc ctggccgtgg
    49861 ggttgatcgt cggcttcctg gcggcgaagg cggggcggaa aacgccgccg aaaccggttt
    49921 caggccggga ggagaagccc gcggccgcaa cgaagaccgg tccgccgacc gatccggaaa
    49981 aatgaaggcg ttcttcactc atctgaaaac ccgtattttc cggggttttc tcgttctttt
    50041 gccgctggcc ctgtcgtttc ttgtcctgca gttcctctat atcgccgtgg atcagaggat
    50101 cgcccggctg ttcgagcgct ggatcggatt cagcatcccg ggtttcggcc tgcttctcgt
    50161 cctcttcgtc ctttatcttc tcggcttgat cgcctcgcac tgggccggcc gctgggcgat
    50221 cggagtggtg gacaaggcct cgacccgcat ccccctgatc aagacgattt acctgctcgg
    50281 aaaacaactg ggcgcggcct tttccgcgcc ggaaaaacag gggctgaaac gggtcgttct
    50341 ggtcgagcat ttccgtccgg gcgtctggtc catcggcttc gtcatgggtt cgatgaggga
    50401 gaaagagtcc ggccggacgc tcctccgcct ctttgtcccg accgctccca atcccacctc
    50461 gggctttatg ctgatcgttc ccgagtccca ggttcgggat gtggattgga gcgtgcccga
    50521 ggcgatgaac gccgtcattt cgggtggatt gatcgggccg gaacaggtcg agacgcggcc
    50581 ctaggatctc aaggcttttc tcaggtagcg ggccgtgatc gaggctcggg aacgggccac
    50641 ggcctcgggc gtcccctcgg cgacgaccgt tcctccgttc tccccgccct cgggccccag
    50701 gtcgatgacg taatccgcgc ttttgacgac gtccaggtta tgctcgatga ccaggagcga
    50761 gtgcccttcc tcgaccagcc tctggaaaca gcggagaagc gtcgacacat cctcgggatg
    50821 gagcccgatc gtcggctcgt cgaacagata gaagatgtta cgatcgcgct cgtggatgag
    50881 atgaaacgcc agtttgatcc gctgaagctc gccccccgag agggtggtcg tcggctggcc
    50941 gaggcggaga tagcccaggc cgacgtcctg gagcggccgg agcttcttga cgatttccgg
    51001 ccggtccccg aagaattcca gggcgtcgtc gacggtcagg tgaagcacgt cgtcgatgtt
    51061 ctttccccga tagcggatct cgaggacttc gcgcttgaac cgtttcccct ggcagccctc
    51121 gcagacgaga atgacgtcgg acaggaactg catctccacg atctgccggc ccgccccctc
    51181 gcaatcctcg caccggccgc ccttggtgtt gaaggaaaac gtccctggct tgaggccggc
    51241 gagcttggcc tcccgggttt ggctgaacag gtcgcggatc ccgtccatgg ccttcaggta
    51301 cgtggccgga atggagcggg gcgaggcgct gatgggcgat tggtcgacgc agatgatctt
    51361 gtcgatgcgc tccaagcccc ggatatcggc gaaggcgtcc cgcgattccc cgctccagcc
    51421 ccggtaaagg acgtcatgga gaagcgtgct tttccccgaa ccggagactc ccgtgaggca
    51481 ggcgaacacg cccaagggaa tgcggatgtc gatgcgtttg aggttgtggg cccgggcgtc
    51541 ccggaccagc aggaagccgc gcggctcgcg ccgtcggcgc gggacggcga tctctttctc
    51601 gccggtgaga tagcgggcgg tgatcgaggc gccgtcggag ggcggccgac cgccgtcggg
    51661 ctttttcagg atcgtggaca gcggtccggc gaaaaggatt tcgccgccgt tttccccggc
    51721 ccgggggccc atgtcgatca gatggtcggc cgcccggatg atgtccggat cgtgctcgat
    51781 cacgaggatg gtgttgccga tccccttcag cgactggaga atccggacga gccgggcgtt
    51841 gtcccgggga tggagtccga tggagggctc gtccaggacg aacagcgtcc cgaccagcga
    51901 ggagctgagg gccgcggcca agttgatccg ctgggcttct ccgccgctga gagtgaaggt
    51961 catccggtcc agggtgatgt aatccagccc gacttcgatc aggtacttga ggcggttctc
    52021 gatttcggcg acgagttttt cggcgatctt cgcttcctcg ggggagaggc ggaggtggtg
    52081 gaaaaagacc gcggcttccc cgaccgtcag acggacgatt tccccgatgg acaagcctcc
    52141 gatcttgact ccccgggcgt ccgcgttcaa gcgcgtttgg gcgcaggccg ggcagggggc
    52201 gtatttgcgg taacggctga gaaagacccg gacctggact ttatattttt tcgcctggag
    52261 ccagtcgaag aagcccttga tcccgtacca cttctcgtct ccctcgaaaa ggaagcggcg
    52321 gtgctcggct ttgagatcac ggaaggggat ccgggtcggg atcccccgtc gccgggcctc
    52381 ccgaagcatc tcccgcatga ctccccggga gaccggcttc gtccacggct cgatggctcc
    52441 ctcctcgagg gatttggccg gatcggggat gattttttcc tcgtccagga cggctaaatc
    52501 gccgaatcca tggcattccg gacaggcgcc ctgaggattg ttgaaggaaa agagattcgg
    52561 cgtcggatcc tcgtaggcga tccgacactc cttgcattcc aagctgtcgg aaaaggcgaa
    52621 cgaccggccg tcttccgttt gaacacgggc tcgtccccgg ccgtgcttca tcgccgtctc
    52681 gagagaatcg gccagacgct cccggtcgtc cgggtcggcc ggaagccggt cgacgaagac
    52741 cgacagggtc cggcccttga gggatggaag ctcgtcgaac ggaacgatcc ggccgtcgac
    52801 cagggcccgg aaaaagccga gcttcttgag ggcggccggg cttttttcga tcacggcctc
    52861 aaatgagatc gaaagcggcg tgccggcggg gagagccagg agcttgtcca cgacggcgtc
    52921 gatggtgtcc cgggtcacgg gccggttgca gcgtcggcag tggaccgtgc ccacgcgggc
    52981 gtaaagaacc cggaggaaat cataagcttc ggtgaccgtg gccacggtcg agcggggatt
    53041 tttcgtcgcc gctttctgtt gaatggcgat ggcgggggga agcccttcga tcgtgtcgac
    53101 gtcgggcttg tccatccgct cgaggaattg gcgggcgtac gaggagagag attcgatgta
    53161 ccggcgctgg ccttcggcgt aaagggtgtc cagggcgagc gaggatttcc ccgaaccgct
    53221 cactcccgta acgacggtga aggcgttcag gggaatgtcg acgtccacat tcttgatgtt
    53281 atggacgcgg acaccccgga ggatgatctt gtcttcgccg ctcatggagg ccccattctc
    53341 gtgttgaaaa aagaatcatt atagcacggg cgcggagaaa tgtggataat aggaccatgc
    53401 ggccggccat gaaaaaggcg ttgttttgcg gcgccgtgcc ctttgtcgcc ctggtcgccg
    53461 cccttccgtt cgtcaatcgc ctccggccgg tcgtcctcgg cctgccgttc gtcctgttct
    53521 ggatcctggg ctgggtggcc ttgactccgg cattcctgta tctcgccgac cgattcctcc
    53581 ggaaagataa aaacggcaag gacggcaagc cgtgaccccg gccctggcga tcatcctggg
    53641 agtcatcctg gcggccgcgg ccgccggctt tctggccggc cgccggatcc cgatgaacct
    53701 cgagaactgg acggtcggcg gccggaagtt cggggtcgtg atcatctggc ttctgatggc
    53761 cggagaggtg tacacgacct tcaccttcct gggagccagc ggctgggcgt acgccaaggg
    53821 cgctccgacc tactacatcc tgatctacgg cgcgttggcg tacgtcttgt cgtttttcat
    53881 tctccccgag atttggaaaa tcgggaagaa agccgggctc cacacccagc cggacttctt
    53941 cgtccatgtt tttcaaagcc gcggcctggg gatcttggtg gccgtcatcg ggatcctctc
    54001 gatcttgccc tacctgcagc ttcagctggc cgggctcggc ttgatcgtcg aggcggcatc
    54061 cgacggggcg gtttcctcaa cggccgcgat tctcctctct ttcgccctga ccacggcctt
    54121 cgtcctgacc agcggcctga gaggaagcgc ctgggtttcg gtcatcaagg acatcctcat
    54181 gctggcggcc gtcggtatcg tcggcttcgg cctgccgagg atccatttcg ggagcatcgg
    54241 cgggatgttc cgggccctga tggacaaggc ccccgggcat ctcgttttcc cgggggccgc
    54301 gccggggatg ggcgttccgt gggtgatgtc gaccgttctt ttgaccgcga tgggatttta
    54361 catgtggccc cagctttttg ggacggcctt ctcggccaag agcgaccgga tcatccgccg
    54421 caacgcggtg atcatgcccc tctaccagat accgatcgtc ctggtttttt tcgtcggctt
    54481 cacggcattc ctgatcctgc cgggattgaa aaccggggat ctttcgctgc tgatgctcat
    54541 ccgacggacg tatccgccgt ggttcgtcgg gttcgtcggc gcggccggcg cggtgacggc
    54601 gatggttccc tcctccgttc tcgtgctgtc ggcggcggcg cttctttcca agaacgtcta
    54661 ccgggccggc ttccgtcccg gggcctccga cgcggcggtg atgcgggtct ctcgctggat
    54721 ggtcctggcg gtcgccacgg cggccctggt tttcgccctg gtcctgccca atgccctcgt
    54781 caatctcctg ttgctcggat acgacggcgt ggcccagctt tttccgggag ttgttttggg
    54841 cctcttttgg aaaaaagtca cccggagggg cgtcaccgcc ggcttggccg cgggaatcgc
    54901 cgccgccgcg ttcctcgtct tcgggaaaca cgatcctttt tgcggcctca acgccggctt
    54961 cgtcgcgctc ctggtcaata tcctgaccgt gacggtcgtg agcctcgccg gccgacgccg
    55021 tccggcgcct cgcgcaggct gatttacggc ccgttttctg ttagattaag agcttcccct
    55081 gggagcgccg tcatgtatga agacataaaa aacgaagcgg aagccctttc cggccggatg
    55141 tcggaatggc ggcgcgcgtt ccatcgccgt ccggaaacgg ccaacaacga gcatgaaacc
    55201 tccgccgcct taaaggcttt tttcgagcgg ctcggccttc ccgtccgccg gctcgccgga
    55261 acggggcttc gggccgattt ggacggcctt cccggcgggc ggacggtcgc cctgcgggcg
    55321 gatatggatg ccttgccgct tgaggaggaa ggggagaagc cttaccggtc ggagattccc
    55381 ggcgcctgcc acgcctgcgg ccacgacggc cacttggccg gcttgatggg cgcggcccat
    55441 atcctggccg cccggcggga caagatccgc ggccgggtgg tctttctctg ccagccggcc
    55501 gaggagcttc cgcccggggg ggccggcccg atgatcgagg ccggagccct cgagggcgtc
    55561 gacggcattt tcggaattca tctctggcaa actcttccga ccggcaagat cggcctggtg
    55621 aaggggccgg tcatggccca ggccgacaac ttccgcatca ccgtaaaggg acggggcggc
    55681 catggatcga tgcctcaagc cacggccgac ccgatcgtcg cggccgggca tctgatcaca
    55741 gccctccagg gcatcgtcag ccgcaacgtc gatccgttga agccggccgt cttgtcgttc
    55801 gggacgatcc ggggcgggac ggtttacaac atcataccca acgaggtcca tctcagcgga
    55861 acggtccggg cgttcgagcc ggccgtccag gagttgatga aaaagaggct gggcgaaatc
    55921 accgaggcgg tcggccgggc cggcggagtc caggcgttcc tcgagtacga ggagggatac
    55981 ccgccgctgg tgaatccgtc cgcctccgtt gatttcgccg cccaaacgat cgaagcgctt
    56041 tgggggcggg aggttttcgc gcccttcgcc ccggtgatga gcggcgagga tttttcacgt
    56101 tatcttcgga aggcgcccgg agcgttcctt ttcttgggga taggcgacgg aatgccctat
    56161 cctcatcatc atcccggttt cgacctggac gagcggtccc tgccgcgggc ggccgctttg
    56221 ctggcggcct tggccgtttc gtttttggaa cagccggaat gagcttcggc gtcctcctgg
    56281 ccttagccct ggcgatggcc atggacacgt tcgccgtggc catcggcgtg agcctggtca
    56341 tgaagggctg cacgccccgg caaacgttcc ggctggccgg gagcttcggg tcttttcagt
    56401 ttcttatgcc ccttctcggc tggaccgtcg gccggaccgt cgtcgatctc gttcgggggt
    56461 tcgatcattg gctcgcggcc gggctcctgg ccgtggtcgc cggcaagatg atcgttgagg
    56521 ccttggaaaa aaagaaagac ggaaaagcgg ccgggccgtg tcccgacaaa acgcggggag
    56581 ggcatcttct ccttttgtcc ctggccacca gcatcgacgc cttggccgtg ggattgagcc
    56641 ttgcggcgct gaacgtttca gtcgtctatc cggcggccgt catcggcctc gtcacgttcg
    56701 tcgtctcggc cgtgggaacg aaaatcgggc cggttttggg tcgctgggcg ggcaagagcg
    56761 cggaattggc cggcgctctc gtcctcctgg ccatcgcggc caagatcctc gtcgagcatc
    56821 tttcctgagg ccggcggcga ggggaacgct tgcccacccg gggcggcccg cccgggatct
    56881 taaaaaatcg tgtagcggcc gtctttcatg atcatttccg tccggccgcg ggcgaaggaa
    56941 aggaccacct cgacgacggc gacccggggc tgcatttccg ggaggtaaat gcggtcgaga
    57001 tggatgaccg cggccggcgt ggaaaaatcc ttcaagccga tccgtccgcc gaaatggtcg
    57061 ctccggccga aggcgatgtg aaggccgagc ttctcgtcca ggagaatctc gccgcaggga
    57121 ctgaggccga atttccccaa aaccccgaat cccagctcgg ccatgttgcc gtaggccggc
    57181 tcccgcttca agtaatccga ttcctcttgg gctgtcggtc cgccctcggc ggaaaccgcc
    57241 gtgttcttct caatcttgta gtaaacgacc tcccccccga tttccacggg caaaacgccg
    57301 gccgtggcgc tgggggcttt cccctcccct tcgtagggaa cgatgtaagc ctcgccgctg
    57361 gggaggttcc cggccgttcc gatctcgggg aagcggccgg tgctcgagtg ggcggtccgg
    57421 tggcggaggt cgaaatgcat ccgggcttcc acccggccgt cgacgaggaa aaggatgtcg
    57481 gcgccgacgg ccttgtcgag ccgggccttg acggcctcga cgtatcgggc cacctggacg
    57541 taatcgaccc tcagggcggg gatcatctcc ggtccgaagc cgggcatcgt cgccgcccgg
    57601 aagccgtaat gcttggccgc cagcttcaag ggggcggtcg tcgagtactc ggtgggagcg
    57661 atgaggatct gagattcgga gaagacgtcg gcgaacggaa cccggagaga cgtttccttc
    57721 aaggaggccg cctcgtctgg aggaatcgtt ccgcagagat aggcgtcggc cggcagg
//
LOCUS       DAOOXH010000007        55537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_478_length_55537_cov_7.557965, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000007 DAOOXH010000000
VERSION     DAOOXH010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 55537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55537
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_478_length_55537_cov_7.557965"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="PKV64_01910"
     CDS             complement(<1..1444)
                     /locus_tag="PKV64_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15866.1"
                     /translation="MIKRLGRGFGLLLAAALCLYVYLGERAAGRARNTALGEGTAAGE
                     TGGRFPWLNDGLYHARANALFKSGVGRLGEVEERDADFRLAYRNYVRSLDLHPFSPTA
                     HFDFGQLLQYLNALDFPAAERYFDEYRKAAALSGVDTLINFEVGKILLARWPSLSPGE
                     RRFVEDITRSLLSAGGPGREERLDLILSLWEINVRDTAVLEEILPPESDVLRRAAGFL
                     GGKGIFPAARLGFLAEAEHLDFRRAVREAQAGLIASHAPRPDESIQHYRAARTLLEGI
                     RFYQRLSPKTEPVPAEEYGKLAKAVHLGILKSRLAAGADLRAVHDDFRKALDAEDGVG
                     AAAELESLFKSREMVEDRSRSGLLDIERLGVSMELAFKQNRFREVVQTGQTLRQNLLV
                     VPDERRPWIGRMYELAGDACQRLDDLYESNAYYEKALEWGAEAAVVRIKLRRNYERLN
                     MARDIIDLQKQIEAGLTPRETWLGDSIWTAG"
     gene            complement(1446..2936)
                     /locus_tag="PKV64_01915"
     CDS             complement(1446..2936)
                     /locus_tag="PKV64_01915"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPN15867.1"
                     /translation="MWIVRIRRFGFRGEPREAGPDEYDQNGLSLRDRRGRRILEVGLL
                     ALLAWAPLPIASVAPWSVLVIEIAAFALFAVYLSLERKPRASPKLISDMRPLRPAFLG
                     LGIFLAFQMLPLPDAVVRILSPRTAALRAEYVPVSARTGMGTLSLLPGYTLAASLELL
                     AYILIGVLVVRTVTHRKQIRRIMVTLVGVGVFEALYGLFELYRSHPRLLFYAKEHNLD
                     SATGTFVNRNHYAGYLEMMIPLALGLIVSRLDLFGEPGTPFRTRFARFLNRGLGLNLF
                     LGIGLVVMAAALFLSNSRSGFVILLFSFGLMLVLTAQYFGHVLVHQAWVRRVIGVAAA
                     AVLGFGLYVGLETMIGRFALDKLLQDGRPRYWGGVMTMIGQFPLFGVGFGAFGGVYQA
                     YDTTGMEYALVHAHNDYLEFLSELGVLGFGLLAFVAGLLLFKTFRMWMTRRHPELKGL
                     ALGGLASAAAILTHSMTDFNLQIPANALTFSVILALTMKTAYHRKT"
     gene            complement(2945..5251)
                     /locus_tag="PKV64_01920"
     CDS             complement(2945..5251)
                     /locus_tag="PKV64_01920"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HPN15868.1"
                     /translation="MTGARNVNRQDINLGEYWAIVWKRRTIVFSTLGACLVLAAVLSF
                     TQTRRYTATAQILIEDQGSGRFNLQEYLNAPTNTTDDFLGTYFNTQLRIIQSRSLAER
                     VVRKMNLGARPEFLAEVEKNRTMADMAKYILTFRWLRPKGRPAEPGAGKTAGAEADAL
                     YAGRLLERLSVYPVPETRLVDVSYVSTQARLSADVVNTLVQEYIDYSIESRSEATQQT
                     SDFLTETIAQLRQDLDAKERQLQRYSEEKKILPLSDKDSNTASQYASAAGAYSSAQVA
                     RIAAQAKYQELQKLRVDSLPQNVSDPTIQALRTNYYQAKSEYEEKIATTYTPSHPEMI
                     QLKVKVDTLESQLKTEINKAVDLAYSEYSQALGNENRLRDMLESKRSEVTRQNNDSLF
                     YAGLNTEVESLRTLISTLTAQQKTTQVSTMLSGLKTSNIKIVDRALVPDEPSSPNVKR
                     NLIVAFLLGLLLGVGLAFAAHFLDNTIKNPEDLDKLTGLPSLGLIPHFSPRDTGGKGV
                     YAAAYGDAGSQSLEAARISEIELINHLFPKISIAEDYRNVRTSILFSRIDSDQRVIAF
                     TSTQPQEGKSATIANIAISFAQMGERVLAVDADLRKPRLHKIFQVRNAVGLSDVLTGR
                     VDLAETVQKTPIAHFSLLPSGPHPPNPAELLNSKKMKELLSIVRESYEIILVDLPPVL
                     AVVDPVIVAAMADMTILVLKTGKTTRKPLLRAIDQLAKAKARIAGVIFNDAEMKKGDF
                     MTPYFQYEYYQDAGVDEVVRQKKTEKKD"
     gene            complement(5429..6289)
                     /locus_tag="PKV64_01925"
     CDS             complement(5429..6289)
                     /locus_tag="PKV64_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPN15869.1"
                     /translation="MRLPTAAAAWIVLFLIVIGVAAAQDPAGQAERFVTAYRIGPKDL
                     LEIKVREDEKLNTEVRVSEQGKVNLAYINEVYVEGLTAAELGDKLAELYRPFLRDPHV
                     AVTIKERQSKQVSILGAVTNPGFVQLFGRTTLLEAITAAGGLTREAAREIIILRTFPD
                     GTTGNLKIPVDDLMLRGDPKYNVPLEAGDSVIVQIDRTVHVYIYGQVKIPGALSVLQS
                     RIPTLTQAIAQAGGFTERAAKRRVTVTRKDENGREKTFEINVVRIQNNRLEDFPLEEG
                     DTVYVPDTIF"
     gene            complement(6303..7541)
                     /locus_tag="PKV64_01930"
     CDS             complement(6303..7541)
                     /locus_tag="PKV64_01930"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPN15870.1"
                     /translation="MRRLPVLIFFCLGLAGALSAQSYQVFADEYRRLTEQAFLRLGPL
                     RLFPSLGVRDAGFDDNLYFGSDDGAPKKDFTATLSPEIRVYWPLRDVLILTFRENPEY
                     AYFQRETRQRGFTNSYGAGFRSLLLRRFVLSGAFGNDVHRRRLSSEFQIPANDRVRSL
                     EAGLSYETARKTSIGITGAFRRISYEEIRIDPADAPVSRLLDREEREIGAEAYYQLFP
                     DGFLFLRGDLREYRFRDETAAWRDAEAVSFWAGLRFPLLGRIRGILNLGYKNFNPNRA
                     GEPPFRGLVGNTGLDLRTGRLAFRLDFARDLVFSTYADVFYFVENSWGAGGSLYLTSF
                     LKIDYDYSGGTADYSERAAGEDSGNRVDRRRNHSLGLTVRVLRTAGIGIAWNSERWTS
                     TLGSFDRARNFVGLTYTRYF"
     gene            complement(7562..8752)
                     /locus_tag="PKV64_01935"
     CDS             complement(7562..8752)
                     /locus_tag="PKV64_01935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPN15871.1"
                     /translation="MDNADVAVIGGGPAGLQTARFLAEGGADVTVLEAKPAVGANVVC
                     TGLIGRDVFSEFRLPAETIGPDFQEAEIVSFSGRSIRYRHPSPFASIVDRERFDGNLG
                     GLAREAGAEIETGTMVRDVSVSRDGVTIEAERDGRPVRRRVRLTVLATGVEGRLHAKL
                     GLTPPARRIYGAQVEIRGEGSVAPTIFVGPPTAPGGFAWSVPSGGDRFRVGLITDGNP
                     RKVFDDFAARYYPRLDPAGAVRRLEFKPICQGMAKRSVADRALAVGEAAGQVKTTTGG
                     GIYYGLWGARLASETILEALKKNAFGPADLAAYDRRWKAGLRREISIGYYARKFYSMM
                     SAGQFEALFTLAQSDGLIPVIQNEGQFDWQSGLILSLFRKTAVAGIFKGLSRNNVFLD
                     RLLN"
     gene            complement(8849..9478)
                     /locus_tag="PKV64_01940"
     CDS             complement(8849..9478)
                     /locus_tag="PKV64_01940"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPN15872.1"
                     /translation="MSAAWRKALFLKRPFDAALSFFGLFVSSPLWAVIAALIKIEDGG
                     PVFYKQDRVGKDGRIFQALKFRSMRTDAEKDGPQQAFEDDPRVTRVGRILRATAMDEL
                     PQLFNIFKGDMSFVGPRALRPNEKEVRGDPDQRDIQDIPGYRERHVVRPGLTGLTQVF
                     LPGDTPRRAKFRYDLLYLRKRSFGFDLRLIFLSFWITFRGKWESRQSKI"
     gene            complement(9475..10710)
                     /locus_tag="PKV64_01945"
     CDS             complement(9475..10710)
                     /locus_tag="PKV64_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPN15873.1"
                     /translation="MRILIHTMYFLPEMGSAPILMNELASSLAARGHRVEIVTTLPRP
                     PHNRGYEGRLFVRETRNGVLVKRYPTNVTSHPAGRLIAWTIYTLAAIWNLRTVRRGDV
                     VFLRLPPLQLGLTGILARLLCGAAVILNVQDIHPDLSIEAGILRHPLAIRAALKFEKW
                     IYDGHRRIVVISDGFRKNLRAKGVPDERISIIPNWVDTDFLRPLPKDNPVARRLGLDG
                     VFTVMYSGIISLSSFETLARILEAARLLAPDRETRLVIVGDGLKRADLQARAEELRLD
                     NVVFLPFQPYADLPGLLASADVLLVPLDKEKSFLSVPSKLYNYLAAGRPILGLAVESS
                     EVYRIIREAECGLCAPPDDPQRIAAAVGALRDDPGGREAMAARGRRLAEDVYSRRSVV
                     EAFEALMEEAARGPRGRRP"
     gene            complement(10698..11933)
                     /locus_tag="PKV64_01950"
     CDS             complement(10698..11933)
                     /locus_tag="PKV64_01950"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPN15874.1"
                     /translation="MMSSVFLWLLVWGGMFTSIDAVGSESFLRSGMGFLQGIRSLFPL
                     AALFFCLLWILKERPAFPFRRNPLLFWYAYAAVGVLSSLFLSQEPLTAIYWAGNYLAP
                     LMAAWFALERPDPWDAVRALIYSNYAMFALVAVSILPEAFRVGWGNLPRFRQYTLMFG
                     LGQINVNGAGRYALTIIIVLAVRFLLGRSRLRYLLIPAALPFLFLLTQTQSRTALLGL
                     AVASVLFVIMRGSDWRFLFVGPVAVAVVYISGFQWRSHGSMDRLMSLTGREYTWRAGL
                     ELIKKSPFLGWGFQADRIMMNSEHMHNSYLHAAIHTGVIGMLLFVAALVSFWGMVARS
                     GILFRAKRFTGERRTLLFESTMIAGFLTARSFFESTAAFYGVDLLLFIPAIACVALAA
                     WTAPGDESPREEAAPACAS"
     gene            complement(11930..13231)
                     /locus_tag="PKV64_01955"
     CDS             complement(11930..13231)
                     /locus_tag="PKV64_01955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN15875.1"
                     /translation="MKRLNVLISAFACHPPLEAGAPEEEKLLASGESILGWNIVNQVA
                     RFHDAWVLTQARNRPGIERAQMRGEAAGVRFRYLDYKLWWKPLWHKSFTLHFYYYFWQ
                     WLAWAEARKLRRTVAFDLAQHATFANDWMPDYIGARLDIPFVWGPIGGGQRNPKGFES
                     EYGWKARAGERYRILSQELGRRLIRGRRRCASRARAILVCNEETRRKIPPADRAKVRF
                     FPVTGINRADLHDPVARPEGDRSFRLLTTGRLVRYKRFDFCIRSTAAFLRNNPGADVL
                     LEIVGEGPEGPALQALVREMKMEEHIRFVPWMPRRELITRMTACDVFLFPSLREGGGI
                     VVIEAMACGKPVVALDSAGPGFHIQPEWGLKVEPRNPEQVVEDLAAALERLWKDPGLR
                     LRLGRAARRRAEDFYLWDRLGERLRDIQAEAVAGPLGPERT"
     gene            complement(13240..14187)
                     /locus_tag="PKV64_01960"
     CDS             complement(13240..14187)
                     /locus_tag="PKV64_01960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPN15876.1"
                     /translation="MKPRLSFLIPVKNVAPWLADAVDGVRSLSSADWEIVVADDHSGD
                     GTKDLASRLAGGDSRIIVIDNPGRGTVAGLNAAYAASSGGHVKFLDGDDALDPGFSAR
                     LPEILAEDASTHDARIVDEKLAPLSLLRLTDRFAVQPYAEVFRGGIVSPPRWAWTFSR
                     RIADVLFPMPDLPSIHEDFWVALVIKKAAARIVHFDEPLYLYRQRAGQIFGGLFNFSP
                     DIVRRRAEAMLLVLEAVEKNSAFFGADVPAFGERLAEMRKYYRVSARPGASTGDILGL
                     RLPLGRTLKLWTVLKAPRAASFVSRFKAGRASRLFSRMK"
     gene            complement(14184..15335)
                     /locus_tag="PKV64_01965"
     CDS             complement(14184..15335)
                     /locus_tag="PKV64_01965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN15877.1"
                     /translation="MKTVFLSLYQAYPPFSGAAAVSYRTARAWPGERVLFQLDDGRTP
                     ETTPDGLRLVGFRLSSTRRLRKLLGLLARFSRIARRVASERPDAVVMEGSSWAPYYAV
                     LHRLIRRRCPRAVFVYHAHNVEWILRRERKDRAVVSGLTKWGERRLLRSVDIATAVSE
                     IDAGAMARLYGVQPRLLPNGVDAAAFERISPAEIEAVRARYGLNGREALFMGLLGYPP
                     NDEALRFLFGIFENVVRANPGVRLAVLGGTVRGSAPPWMVNPGMIPHADVPAVIRSSA
                     VGLAPVFSGSGTRIKILEYAAAGIPVVATAKGAEGLPLKDRVHLRLADGGDAFAAAIN
                     GVYADRGEAEAMAARGAAEVRRLFDWPGLVAPVAAACAALVASKKESGR"
     gene            complement(15332..16897)
                     /locus_tag="PKV64_01970"
     CDS             complement(15332..16897)
                     /locus_tag="PKV64_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HPN15878.1"
                     /translation="MSLHNEPARDAKPDLGSRRLVKNTGLNLLGIVVPAMIGLVTVPV
                     VIRWLGTDRFGIFSIILTVFGYFALFDLGLGRASIKFSAEAMGRGAMEDLPRIIRTTV
                     GLQTVFGLAGMVLMIVLTPLLAERILKIPAANLAEAKGALMIMALSLPFAIVTPSIRG
                     VLEAGQRFELVNAVKIPTNALIYVLPLIGAPLGMGLRGIVLLLTASRVVTLFIWIGLA
                     FRVYPVLARKEGFLPRHRLRKLFSFGGWNALSSFVWFFLVSADRFVLGTLRGMTAVGY
                     YAAPAEATGRMNMLPGSLALTLFPAFSALEGGGDAERSKRLFTRSLKFTLILLGPPAA
                     GLIALAGFILRIWLGPEFALQSRLVLQILSLGFLISALAYVPYSYLQGLGRADLPTKF
                     QLIETAVFIPLLWFGVLRFGINGAAAACTIRVLLDLGLLIEASLRVGKIPLRALAETG
                     IFRIGLILAVFTAALFGAKRLPAAPLWIVLASALYVLWAWRRALTPEERDGWTGLARR
                     FFRGKAAGEAAGS"
     gene            17180..18187
                     /locus_tag="PKV64_01975"
     CDS             17180..18187
                     /locus_tag="PKV64_01975"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN15879.1"
                     /translation="MFILKDGQGNAGPRPGNRSLLLFGLGFLFLLAAVPGFPLSQETD
                     PFYLKWLESGEAAYLARDYRQAVSYLEIAVFGLARDKDLQARGYVFLALSRTALGFKD
                     RAAADLSAADGLVGRDGLRSMNLPEAARMRLDAFLASAPASKAPPETPTAAVTAETGT
                     VPPPPPSEILPSGDRTEATPLPPAETPSTEDLRRAMEANPREPRSYYELSRLNRRAGK
                     TEEAREILETLLARNPAEIRAYLEIGRLDYEKDDFKAAMKALEKFISLSAGVFVEERF
                     RDEGRALLLLSASRRGEARKTARLLEESEELFRPERFAKLTLDPADLERLRRLQRTSA
                     G"
     gene            18252..19559
                     /gene="thiC"
                     /locus_tag="PKV64_01980"
     CDS             18252..19559
                     /gene="thiC"
                     /locus_tag="PKV64_01980"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HPN15880.1"
                     /translation="MTQIESAKKGRVTAEARAVARLEGVPVESLMESLAAGTVVIPHN
                     RLHRPFRPAGIGRGLRTKVNVNLGTSRDFPHLPAELQKTRISLKYGTDALMDLSTGGD
                     IRKIRRAILKAAPIPLGTVPIYQAAIEAIARRGTIVDMTVDDLFAAIEAQAREGVDFM
                     TVHCGLTLRAIDRLRGQGRIADIVSRGGAFHVAWCLHHGKENPLFEHFDRLLEIARRH
                     DITLSLGDGLRPGSIADATDRAQIEELLTLGGLVRRARRAGVQVMVEGPGHVPLDQVE
                     MNVKLEKRLCEGAPFYVLGPLVTDIAAGYDHIAGAIGGAIAAAAGADFLCYVTPAEHL
                     GLPSPEDVRDGLIASKIAAHAADLVKGVPGARERDERMSRARKKLDWKTQGRWAVDPE
                     KFRSVRAARRTHSPACSMCGEFCAMRIVSEYLGGRRAADDSRC"
     gene            19543..20394
                     /locus_tag="PKV64_01985"
     CDS             19543..20394
                     /locus_tag="PKV64_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPN15881.1"
                     /translation="MTPAVRGLHFGTAGVPDSTPQASTLAALPHLKSIGLDALEIEFV
                     RGVKIGSDTAAKVRRRAEELGILLSAHAPYYVNLNSREMGTRLQSREHLLRSARMGAL
                     CGCRSVVFHAGYYGSATPEQTFDVLREALREILSVLKSERNPVTLRIETMGRRSQFGS
                     LDEVVSLCRDVEGLAPCLDFSHLHAREGKANSYIEFHRILRKVERRLGRQALRDLHVH
                     LSGIAYNDKGEIKHLNLADADFRYEEWIAAVNDMNAEGLVICESPAREHDAAVLKRLY
                     ETKRLKS"
     gene            complement(20419..22116)
                     /locus_tag="PKV64_01990"
     CDS             complement(20419..22116)
                     /locus_tag="PKV64_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15882.1"
                     /translation="MRRSSDSPAGALSAAAAVIVFAAAVLIVFPAATQTGDSLEYARA
                     VRSGTNLFHPHHLLFNAVVRGFWLGLRSVFPSIDPIAAGQVHNILWALAALAAFFTIV
                     RRLTGSTGAAAVFSLALFSALGFWQYATFVEIYIPSLGCLAVSLVFLLSPRPGPPGPG
                     ARTAATGFFVLAVLYNQMAVFFVLALAVLLTPRFSAREARRTGRLITAAGAIVLGAYA
                     AVFLSAARPAASEGFVRWCLSYAFNPDPTWGSFSNVSLLGLCKLFLSFARDVLFIPRF
                     LLVPASIVSGVLCAGLAVPVLRSIARRKPAANARIALVLWILGTALFMWWFSPAGYEL
                     SIPLLLPAFLILALISADGWEAAGGGKARRLILSGAAAVVLFVFLFNLTAAVLPAHAS
                     RGVDYSRAGLIEKNVPAEATVFADFWLEENLRYYFNRTAVIEEGPVLFSFYRFKDLSP
                     DMIPDPDRPVVASVELLSPRTRPEGLFGGDVQPLEWRAFIEWICGCEIRDGRVTAART
                     PSPLAGLPEYLLLSGERKPVEGLAGLFRLLDAAAAAAAPEFSGSFSAWLERHPDAAR"
     gene            22311..24887
                     /locus_tag="PKV64_01995"
     CDS             22311..24887
                     /locus_tag="PKV64_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HPN15883.1"
                     /translation="MKRKLAAAALVLLVVLLLPAQQKRIYIANDDHTDYLWAADANTY
                     RDAFLAMLDYYLDQAEATAADPEPYRNRFNVDGTFWIRTYENNRSPAEFDRLVLRIKD
                     GSIGVPITLLNLCYGAMPAEAVLRSMYYAGRLERRYGLRLKLALSQENQTLPLGLGTL
                     FSGAGASFFWKGICGCASRVPDAWDRPLDMYWWTGLDGSRILTKWNSMIRGNYYDLGG
                     YAEARNLPEAVSFVTSDARFKARHPYDVIGIFGYGGDDLKSFTDQFPAAAETLTTADR
                     RIIVSNINDFFEDFAAAHGSGLPAQTAGFGNEWDILSASLAEVSGRVKRATEKLRTAE
                     AMAAMVSLQDSSFMRGREGAGESAWFDLGLYYDHDWTADGPITRDQRAAWQRERAAGI
                     ESYVDTLDKDARSALGGMIEKSGSNPRYFVFNPLGWTRTGEADLPAGAAEQSVRVIDT
                     STGLEAPSQRVVLDGRAYLRVWTENVPPVGYKTYEIAPGTAAVFDGRPTAGGNVLEND
                     RYRLVLGTNGAILSLIDKRRGGREMVRPADGRMINDPGPGSGTIEAENTGNVSASLRI
                     KSSSPLVHETVVTLYRDSSRIDIRNEIRQNFSGLQTWDFNVNLDDPLLRHEEIGAVLL
                     ARRTVDGGHYSPKAARTDWLTLNHFADLSAGETGLTVSSPDLSFVRRGTSTPTFLDTR
                     TPRISILAGGQVDGPGLGIPSQGGDSFFLQRFALETHGAFDAVEAMRFALEHQNPLTA
                     GEVTGGTKYPAAAFSLLTPTAPGVFIWAVKPAENTARGGIVVRLWNQTAAETSFSLGF
                     NGFEILNASRTSLIETPLETASFLGSLLSGTVPGFGWRAYHLELSGDEVIGKGIGRKI
                     IR"
     gene            complement(24890..25540)
                     /locus_tag="PKV64_02000"
     CDS             complement(24890..25540)
                     /locus_tag="PKV64_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15884.1"
                     /translation="MKSRAVAWGFAAALLAAGGTAEARDRMNSLTVYGGVHHHFPSGK
                     AEEYVLGENDFPVVPAHNGILAGFSLIRMFGRTLGAEFDARFVGTSPVVLTDPSDGDI
                     VELTAGPHVSVTLGVFLAPFSGAVRPYLSAGGGLDASLAGAASSITRYGFVIEVPAPA
                     FKDRFDPEAHAGAGLLLLPGKGRTWGFRLDSRYAWIFDGSRTISGLSGSAGFFLVF"
     gene            25714..27609
                     /gene="asnB"
                     /locus_tag="PKV64_02005"
     CDS             25714..27609
                     /gene="asnB"
                     /locus_tag="PKV64_02005"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HPN15885.1"
                     /translation="MCGLVAFFNKDKQAADPSILRRMAERIAYRGPDEEGELVEGPLG
                     LAHKRLAVIDLQTGRQPMTVEDVTVVFNGIIYNYLELREELGRLGRTFTTASDTEVLL
                     HAYLEFGADCVKHFNGMFAFVLYDRRLRRLMAARDHFGLKPLYVHEAGGAWLFASEIK
                     ALLAHPGVAAAVNEEAIRDYTVFQYVLGGDTFFKDIRKIPPGRYLLIDTESGDVREES
                     FWDLDFTVDTYHTKEYFLDRLRGLLEDSVRLQLRSDVPVGTTLSGGLDSSLVTLLAAR
                     QSPQPLVCFTGAFRESSAFDETPYAREVVRLAGAESREIYPKMEDFIDTLAELVYHMD
                     EPAGGPGLFPQYIVAREAAGRVKVVLNGQGGDEIFGGYTRYVVAYLEQALKGAITETN
                     DEGEHIVTLKSILPNLPALRRYTPMLRRFWRDGLFDPMAARYFRLIDRNAGDRSMYTA
                     EFRAGFDDDRMFERFCSVFDHPRTLSYYNKMVHYDLKAGLPAILQVEDRMTMACGLES
                     RSPLLDRRLAELVASIPPRLKFEGGDLKHILKLAAGDVLPSRILERKDKMGFPVPLHL
                     WIQNGLRAYIRDILLSKACRERGIFDPAAVERLIENEDAYGRKLWGLLNIELWHLRFI
                     DRRGGTP"
     gene            27609..29303
                     /locus_tag="PKV64_02010"
     CDS             27609..29303
                     /locus_tag="PKV64_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPN15886.1"
                     /translation="MNTEKIAVVGAGVVGIPMAAALASAPPPDASAKPARVILIQRNS
                     STSGWKVGAINAGRSPLGGIEPGLEPLIRRAAAAGTLQATSDFSALRDAGAILFCVQT
                     DKRGLAPDYGPLMEAVSATIRVLRETPAGRRTLVVFESTLAPTTMQTVMRKTFTDHGL
                     ADGRDVLLANSPNRVMPGFLLERIRTSDKLIGGLRSETAPAVARLYSPLVSGGRLHLT
                     NCLTAETVKTFENAYRDVRIACAAELVRLCDKMDVDFHALRGEVNRRLAWEDGASADA
                     ALVPVGGLLVPTIGVGGHCLPKDGVLLLWRMIEAGQDMSASLFLQSRRINDASPAWAA
                     DRLEDLWGPPAGKKIALLGTAYRPNSEDTRNSPTLALAAELAGRGAAVVLHDPYVRPV
                     DQNLERTGLDGSFTRDLDHALFGADGAVICAAHDFYREEWPRILRSFPGKPAVFDGCH
                     LHSRAESPDVPGLGKGRAAPPPDLTGFALSSFRAVERGVALEMEAFAEFINADAPDAG
                     SRLDLNEAGRLAATCVTGCRLAAPAPVDALPVFQGSFLSLAEKSLELSRRREKNNP"
     gene            29300..30268
                     /locus_tag="PKV64_02015"
     CDS             29300..30268
                     /locus_tag="PKV64_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017288806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPN15887.1"
                     /translation="MSERKAAGISFDLDNLWSYLKTHGDPGWDTYPSYLDRLLPDVLD
                     LFGRRGLRVSFFIVGRDAALEKNRAVLKLIAEAGHEAGNHSFDHEPWLHRKTKPEIEA
                     EIGRAETAITEATGLRPRGFRGPGFSWSPDLIEVLAARGYLYDASTFPTFIGPLGRLY
                     YFARSSLSERQREERRKLFGGATEGLRPVRPYFWRLRGGTTLLEIPVTTLPLLKTPFH
                     LSYLLYLGAISPAAMRTYLRTALAACRTSRTGVSFLLHPLDFLAAADAPGLGFFPAMK
                     LDRRKKRALVLDALDIFSEYFDILPLGAYAERCFDAPAALPRKALR"
     gene            30265..31380
                     /locus_tag="PKV64_02020"
     CDS             30265..31380
                     /locus_tag="PKV64_02020"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPN15888.1"
                     /translation="MNETDPRPLVSVICPAFNEAAVLERNLDRLDRYLQTLTDYRFEI
                     LIVDDGSRDATGDIAEAFAAGRGDVRVFHHQFNFRLGQVLRTAFHGSQGAIVVVIDAD
                     LSYAPEHIGRLIEKMRTSRAKIVIASPYAEGGRTVGIPFLRRILSRTANWFLCRMATR
                     DFFSDKLTNITGMVRAYDGPFIRSLSLWAMDVDINPEIINKAKILRARIVEIPAVLDW
                     TEQNARAGGRRRRVSNIRLMRSVIQSLVSGFLFRPFLFYIVPGFLLLILSLYPLFWTL
                     RHTIHFYRALSPNDLSFAFRLSEAIGRAFQMAPHSFIVGGIALLVAIQLISLGLLALQ
                     KKRYFAELFYLASITYRDCSKGEGDRIHLPSPLRKKF"
     gene            31390..32721
                     /locus_tag="PKV64_02025"
     CDS             31390..32721
                     /locus_tag="PKV64_02025"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPN15889.1"
                     /translation="MPPEPASQHLKTIAVVGGGLSGIAAALALARSGRFRVTLFEKAA
                     GLGGLCAPVSIAGIVADRFYHVILASDRATIAFLAHLGLAGEIDWRTSRAGFYGRGRL
                     VPFASTADFLRFPFLSPGAKLRLGWGILRIALSPPPGGANEPVAREWLDRRFGRTVTE
                     NFWDPLLRSKLGDAREKTPASFMDATIKRLFGARRGAGGRERMGAVRGGYAPVLRAAE
                     KALRDSGARLRLGVPVRSVAPAGGALEIQTGEGAEAFDRVLLAVPLPEALRLAGAPAE
                     DPAWARFRRLEHMGLVCLLLALRKSLSPYYLVNLLDPDPPFTGVIESTNVLGPERFGG
                     RHLVYLPKYVASGDPAAGLTDDGVRDLFLPSLRRMFPDLAEEDILEAQVHREPLSLPL
                     PAFGPPLRTGGFHSPVPGLYIGGTAFIKGATVNNDAALKTASEAVETILGE"
     gene            32725..34134
                     /locus_tag="PKV64_02030"
     CDS             32725..34134
                     /locus_tag="PKV64_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019238586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HPN15890.1"
                     /translation="MGLESVFSLKKRGTNVRTEIAAGITTFLTMCYIVFVNPAILAAA
                     GVPFTAATTATAIGAAVVSILMGLVTNRPLALASGMGFNAMLAFSVIGFQQARVPWQV
                     GMAVVLAEGIIILILVLSGLREAVMNAIPLNLKRGIGAGIGLFIAVLGLNQGGLIRPA
                     PLTLVEIGDFSQKHVWVTLAGLLAIIFFSAMGRKSAILIGLGAAAMAALLFGLVEWPS
                     SVIAPLDFSTFFAPFHVVGGKTALVQILTPTLLAAVFSLMLTDFFDTMGSVVAIADQA
                     GFLEKDGRVPGIKGILAVDSLGAAFGGLFGSSSITTYIESAAGVAQGGRSGLMPIVAG
                     LCFVAAAFFSPVIGMVGGGIAIPAGDHYAQFAKAGFTLPGADFLVYPITAGALIFVGF
                     LMMRMVREIEWDRFDEAVPAFLILIGIPLTASISHGIGFGFIVFTLIKFVRGKIRDVH
                     PLMIVVALAFAAFYILESI"
     gene            complement(34127..35695)
                     /locus_tag="PKV64_02035"
     CDS             complement(34127..35695)
                     /locus_tag="PKV64_02035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPN15891.1"
                     /translation="MNSTGPFPRNPPGPKSSARLFLALFLLGAAVRAVDVFRPADGRV
                     RESWRECDYAAVARNFHREGMNILRPRIDWRGDGPGFAEMELPVLPWAMAALYKVFGL
                     HEEIGRGLAWLVSLLMLLVFFALARRLLRPPGDAAASLFFVLSPLAVRVSNALQPEGL
                     MLLFLLVGLLHFLRWLDGGARPSYWISIAATAAAILVKAPAVHIGILFAGLLLWKKGP
                     RAFLRAETWLFGVLALAPAAAWYAHAHGLYLSFGNSLGLSDETHWIGLDLLRAPRTLF
                     SLLLGAARTEGLFVWTPAGVLFLGALLLSPKREEARGIILWWLGAVGLFYLAALRTVG
                     DAWAAYYHVIAVPPAALTVGAVTARSARHRLSSRRDRFRGIVPAAGLLAVFGAEAVLV
                     VHEFHPRDYVSLYSCSAPFAAVIPEGDLILATGGPARDETGRPVAYNASYFFYWLDRK
                     GWNIPREAISPDEVRAYARKGARFFVCEKDVLDAAPGLEARLRAAFPAAAECPAAILF
                     RIEDSGPAPDRISK"
     gene            35858..36412
                     /locus_tag="PKV64_02040"
     CDS             35858..36412
                     /locus_tag="PKV64_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPN15892.1"
                     /translation="MVEIRFHGRGGQGTVVASKILADALAKEGNYVQAYPEFGVERRG
                     SPVVAFIRIDGKPIYDKSRIYAPDHVIVVDPTLVEAIDVTDGLKAGGRILINSDKPAE
                     HFKFPDKFKVTTIDATKIAVANKLGSLAAPIVNTAIVGAVIRVFGFAKLESLLGAIAE
                     AIAIKPEANQKAAAEAYEAAGAIV"
     gene            36424..36723
                     /locus_tag="PKV64_02045"
     CDS             36424..36723
                     /locus_tag="PKV64_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012572439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPN15893.1"
                     /translation="MSKKRDAKKIVYESARDFPQMPMSQGSMLWNKTGSWRNIRPEIH
                     LEKCTQCGICWKFCPDMSIVIENEFPAVDYDYCKGCGICAAECPMKCIVMVEERK"
     gene            36720..37874
                     /gene="porA"
                     /locus_tag="PKV64_02050"
     CDS             36720..37874
                     /gene="porA"
                     /locus_tag="PKV64_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014789079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HPN15894.1"
                     /translation="MRKVIMGNHALSYGAMLSRTQVISAYPITPQTQVVELLSEMCAD
                     GTLQAKFIKVESEHSAMAACIGASIAGARTFTATSAQGLALMHELLHWASGGRLPIVM
                     GDINRAMAPGWSIWTDQNDSLSQRDTGWMQFYVSSNQEVVDTTIQAFKIAEAIGIPAM
                     VVLDAFALSHTYEVVDIPEQDKVDAYLPPYKPYARLTPDAPCAFDGLTGPEHYFELRY
                     KLQKDMEKVPALFDETGREFEKIFGRRLEAVEPYLCDDAEIILVTSGTAGFTARVAVD
                     EMRKEGIKIGNLRIRMFRPFPFDAVRRILAKASKIAVLDRNISYGYHGIFCQEVKSAL
                     NGSGSSAPVFGFIAGLGGRDIMAASFREIVDIVRRQDKPAEEINWIGVKK"
     gene            37871..38770
                     /locus_tag="PKV64_02055"
     CDS             37871..38770
                     /locus_tag="PKV64_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008084648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HPN15895.1"
                     /translation="MSKQKKMTLTLPTEELVGSGNLACQGCGANLALRYVLKAFGKRT
                     AICIPACCWAVIDGPFPYSCLGIPVYHCAFETAASTATGVKAGLEMIGDTETQVIAWA
                     GDGGTFDIGIQSLSAAAERNDDILYVCYDNEAYMNTGIQRSSATPTGAWTTTTPVKFP
                     KDQPKKDIDAIMAAHRIPYQATASIAYPEDLYKKAVKAKNIRGTKFIHVLAPCPTGWK
                     SRPEDAVKLARLAVQTSYFPLYEVEDGEKYTLNFKIQNKKSIDEYLKLQGRFRHLKPE
                     AIAAIQAEVDRNWDRLLKKVTAR"
     gene            38975..39475
                     /locus_tag="PKV64_02060"
     CDS             38975..39475
                     /locus_tag="PKV64_02060"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPN15896.1"
                     /translation="MKTILSAALIAGTLLFSGTGCAAQTPSPGTPAPAFSATDLNGAV
                     LDSAVYKGKILIINFWATWCPPCRAEIPDFAAFYEENKDKGLEIIGFSVDDLPTARLR
                     DFVEKFKMTYPVALADERLVQAFDPGPYIPTTFIIDRKGFIRHKEVGGMDKAALAGWF
                     KRLSAE"
     gene            39546..40787
                     /locus_tag="PKV64_02065"
     CDS             39546..40787
                     /locus_tag="PKV64_02065"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HPN15897.1"
                     /translation="MKRRPRKLFLILIGLIPAAVLPLPGQFHPGQYTDEAPFRTWNSF
                     PYAGAAAAGRGHTVFAWATDASSTSANPALLLTLPKATVALGGSFQWATAMRYGPVNS
                     GLLIASSPIGGSSFTGDHAVVSLRAGRFAFAAGVFLSEIYDRPGAEVYDNENQARLYT
                     IRFTQTGLLRTFHLAAAFQLSPRLGLGAGVNAAAGRLTMELMEDLRFLGFNFTSAKSS
                     SLKGLSINGGIFWDISPSLRAALTFRSPFTLEAGTETVDRYAAPEAGTEIIIRGESAD
                     SFKRPLAVGAGFSIRHSDRFRTALDVAWTRWSSYEAVWLGDAPVRDFRDTVRVSAGGE
                     FLSTIRIFRKIVEAPFRFGLIYDPQPPARPRSAYWGIAAGAGLAIDGFRIDLGGLIGT
                     AQDAGDRLTSARISLSCGYAF"
     gene            40795..42084
                     /locus_tag="PKV64_02070"
     CDS             40795..42084
                     /locus_tag="PKV64_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPN15898.1"
                     /translation="MKRAILLLPVLAGVFVLPVFGGTPDVKIVSVPRSAALAEAAVRL
                     DLDLLAEIEGRVLIAASPSDLRKLAECGLQIVDESVLFAPSSSPASSVYSFSPIGAYH
                     TYRELADDLAALQIQYPDLARVVTIGTSLENRPILALKVSDNPGLDEDEPAVLFLGCH
                     HAREWISVEVPFLFGRRLLEQYASDPAVRRLVDEAEIWIVPMVNPDGHDYSVRVYRYW
                     RKNRRAGEGGLFGVDLNRNYGKAWGCDDEGSSPDPASDVYRGPASFSEPETRAVRDLI
                     LSRRFGALISFHSYSQLLLYPWGYTTLPALQESLLKSLGTRLTDLMRAVNGRDYVCAQ
                     AGAALYLTNGDTTDWAYAATGMPAYTIELPPLDILGGGFFNREEDIDPIFQENLPAML
                     ALVDWAIAHPDPEPDPADPPRRSGRKKNPPGPGIIRR"
     gene            42081..42701
                     /locus_tag="PKV64_02075"
     CDS             42081..42701
                     /locus_tag="PKV64_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15899.1"
                     /translation="MSASLSETSAVSFREGVPYWIFWFLLSLIVLLLFFILLRDKELR
                     RRLSLSLAGTKRRVLRKSLELRLLREKRKKAFLCREIGRTVWGEKIFPDDFRPAFDRL
                     GRIEKEIAARHALLQDLYDEIQKVKTGPGEDRKRRRIRELERKSKAERRRLREKGREK
                     SREYERLGSRTDEMRIEHDSLAEWYVRIDKVNRAILAVLDRIEKTG"
     gene            42723..43304
                     /locus_tag="PKV64_02080"
     CDS             42723..43304
                     /locus_tag="PKV64_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HPN15900.1"
                     /translation="MSKPELLVHICCAPDASYAVPLLETEHAVTGFFYNPNIEPPEEY
                     ERRLAETRKLESLLGFSVEAGPYEPERWLAMTRVFAAEPEKGRRCAVCYALRLDRTAR
                     RAAERGFSAFATVMSVSPWKDARLMNRIGLRLARKHGLRFIESDFKKKDGFRQSVEIS
                     RRLGLYRQNYCGCRYSFRRPAGPAAPARGEQAP"
     gene            43301..45583
                     /gene="hypF"
                     /locus_tag="PKV64_02085"
     CDS             43301..45583
                     /gene="hypF"
                     /locus_tag="PKV64_02085"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HPN15901.1"
                     /translation="MTPPAGSASSALRIDVFGVVQGVGFRPFVVRLARRFGLAGWVKN
                     DARGVQIHVEGGTRSLRGRFAEAVEAERPKLARVERLTRRAVPARGYKDFKVRSSGRG
                     GGFVFISPDISICPACREDIRDPANRRFAYPFTNCTDCGPRYTIVLGLPYDRPRTTMA
                     GFPLCPDCRREYHDPLDRRYHAQPIACPVCGPAVVLRDVRKGTMVPGGIDEAAALLRR
                     GKILAIKGLGGFHLACDPTSAAAVARLRRVKKRTSKPLALMVRDLAAARRIARITAAE
                     AAALESDRRPIVLVGKKIELRGLAPGLKEYGVMLPYTPLHVLLLDRIEMIVATSSNPK
                     DAPIIKDESEGFSEMCDYLLTHDRPIHMRADDSVVRVAGTSPVFLRRARGYVPEPQPA
                     PEGLSGRPPVLALGAELKNTISVFKNGYVVTSQFLGDLDEYRNRMYFEETLAHLTGLF
                     GVKPACVVSDLHPDFHTTRMAERTGLPHFRVQHHHAHVLAVLLEHGLGPGSRVLGIIW
                     DGYGYGADGRAWGGEFLAAGYEGFERYARFEPKPLPGGDLAAREPWRMALSYLWAPGM
                     KAVPAIPSLRGIDRERLRAVWRMIEVGTHAPSASSCGRLFDAVSALAGLAPLRNEYEA
                     EAAVRLEAAAAPGRARPYAFTLTDGSPKTLSFGPMIEEIAAGIRRGESPSVISARFHE
                     TLAAAAVETARAARRDLGLRTVALGGGVFCNRRLLGRIEARLAGAGFTVLRPRRYSPN
                     DESISVGQTAYALARLNPVS"
     gene            complement(45567..46370)
                     /locus_tag="PKV64_02090"
     CDS             complement(45567..46370)
                     /locus_tag="PKV64_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15902.1"
                     /translation="MTFQGVQESFESLKKRFQSSEISKQSFIEEIKKLRLKDEKGRFW
                     TIGFQTGKWYYFDGKDWIAGDPPSQKDKMICVFCGFENDLETEVCARCGGTRGEEPTI
                     CPTCGGPLTKPFQTCPRCNQEPEGVPSLKSIKLMKEDPEEGLFIAKSIRLGSAFLNGG
                     LFGTVLGIFLGAFAGATGSFSEFLPSGLAEQQGKLLGAVFLGLLGGAAGFVFLGAVSF
                     LAAAVLNFVFSVTGGLKIKTGRRPPVPEGSQEEKTGGSAVEALGFNLRD"
     gene            complement(46582..47580)
                     /locus_tag="PKV64_02095"
     CDS             complement(46582..47580)
                     /locus_tag="PKV64_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPN15903.1"
                     /translation="MSLSSEPVPPLSPPSAPAPARRRFSFFAQDRPWINLLLFLLTLG
                     STFFVGLSWSLNFLYAPAFAADPAFRPPADAFRNPRIILLSAAYAAVLMAILLAHELG
                     HYLACRRYGLSATLPFFIPAPSLIGTLGAFIKIRSPIHWKRRLFDIGAAGPLAGFVLA
                     VPAVFAGLAMSRIVPSFPREEAIVFGEPLLLRLAGLLFLRDAGPGTDVVLHPVAFAGW
                     VGILVTALNLLPLGQLDGGHIAYAVFGSRNRRISNIVMGALLVMGFLVWAGWLIWFFL
                     LLILGLRHPRVIDEHEPLGTARTVLAFVLLAVFLLSFIPAPVQDYNVIQMARSLWP"
     gene            complement(47645..47890)
                     /locus_tag="PKV64_02100"
     CDS             complement(47645..47890)
                     /locus_tag="PKV64_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HPN15904.1"
                     /translation="MAFKITEECINCGACEPECPNQAIAAGDTIYVIDPEKCTECVGS
                     HPESQCAAVCPVAACVPDPDHVESKEALEAKYKKLHG"
     gene            complement(47923..48615)
                     /locus_tag="PKV64_02105"
     CDS             complement(47923..48615)
                     /locus_tag="PKV64_02105"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN15905.1"
                     /translation="MKGTERRHLKTDQLVHGMQGVVAFFRKWRSQIVLAGLLILALGV
                     VFAGLRIIRAEQAKSRSRTVSEILALRADLPKVPENAAKLEALAGKGKSGRLASLSLA
                     TYWMEQGRPDKAEAALSSIKDAGRDFQYYQVKSLQAEIALAKGDLDGSLAILAKIVEE
                     NPKDYLLDSVLFRQAEALELKGKEAEAVALYKKLQTEYPQSYYGYEASLKAVRMEGKP
                     GAAALNSSPNGL"
     gene            complement(48704..49180)
                     /locus_tag="PKV64_02110"
     CDS             complement(48704..49180)
                     /locus_tag="PKV64_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPN15906.1"
                     /translation="MAKALVVYYSRTGNTEKMAVRLAGALTKAGLPADLKKAGEAEPS
                     DLLAYDLILIGSPVYYGTMAWPVKKLLDESVKHHGKLRGKVGGAFASSGNPSGGKETA
                     VIDILKALLIHGMIIQGDSKGDHYGPAAVGAPGPRDLKSLDDYAARLAELTRRLFP"
     gene            49251..51863
                     /locus_tag="PKV64_02115"
     CDS             49251..51863
                     /locus_tag="PKV64_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPN15907.1"
                     /translation="MRLTAAVLSLVGFVLPCAGPPYKDPRLPVEERVRDLLARMTPRE
                     KFRQLFMIPGSLDSGTELLKDGIFGLQVEAAESAVETARKINEIQRFFRDRTRLGIPI
                     IPFEEALHGLVHPGATAFPQAIGLAATFDPELTGRVAAAIAREARTRGIRQVLSPVVN
                     IADDVRWGRVEETYGEDPFLAAEMGRAFVAAFEREGVITTPKHLLANSGAGGRDSYPI
                     HWNERLLRELYLPPFEACLRRGGSRSVMTAYNSLDGRPASAQDRLLNRLLKTEWGFQG
                     FIVSDAAAVGGATVLHGTARDYSDAAAQALAAGLDVIFQTRLDQEDLFIPPFLDGTID
                     QAVIDKAVARVLRAKFEAGLFDDPCVDPKAAGEWNGHPSHRALALEAARKSIVLLKNE
                     GRVLPLRTDFKSLAVLGPDAAEARLGGYSRPGNGVISVLDGIRAKIGKTTEIRHARGC
                     GRENIAFAAVPPAVLSCELDGRTEEGLRGEYFDNTALRGSPVLVRADPQIQFQWTLGS
                     PDPENLESGDFSVRWSGRLTAPESGRFRIGVDGDDGYRLFFDGRLLIDNWIKRTRRTL
                     TAEVELERGRAYDIRLEYFESEGGTVFNLVWNVGIPGGGDLEMEEAVRAAASAEAAVI
                     VAGIEEGEFRDRARLGLPGRQEELIRRVAAAGRPTIVVLIGGSAITMSGWLDDVPGVV
                     AAWYPGEAGGAAVADVLFGEADPAGRLPVTFPVFEGQLPLAYNHKPTGRGDDYLDLTG
                     RPLFPFGFGSSYTEFAYDGLDIDKNEISPGGTAAVSCRVTNTGSRAGEEVVQLYVRDV
                     LATVARPIKELKGFRRIFLRPGETKNVRFDVGPETLAMLDARLKKTIEPGDFQIMIGS
                     SSADIRLRGILTVK"
     gene            complement(51882..52736)
                     /locus_tag="PKV64_02120"
     CDS             complement(51882..52736)
                     /locus_tag="PKV64_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HPN15908.1"
                     /translation="MKWRDRRISGNVEDRRRIGGKQVAVGGGIGGIILVILFALLGGN
                     PEDATRILEQGGMETGAGETRPLTEEEIQEGRFVGVVLAETEDVWNGLFQEAGRSYRE
                     PKLVLFSERISSACGFAGAASGPFYCPGDERVYIDLDFFKQMQDRLEAGGDFALAYVI
                     AHEIGHHVQNLLGITDKVMAQRNRLEEEEFNRLMVRLELQADFLAGVWAYYADRTAGL
                     LEEGDIEEAMNAAAAVGDDRIMRQTQGYVVPVAFTRGTSEQRVRWFSKGFQTGDITQG
                     DTFKAGGL"
     gene            53081..54697
                     /locus_tag="PKV64_02125"
     CDS             53081..54697
                     /locus_tag="PKV64_02125"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HPN15909.1"
                     /translation="MKKGVVWGFLGLAFLAGSVSAGGQGPVAVSPGAESGEAVVRQAC
                     PTFSWTSVPWALTYKVAVFEARGGGIVRTYEAMAAQAAPVLVKEIPGQAFSWTPSADE
                     ALLNGGRYVWYVGAMVNAAQGSWSEGRAFVVAEGPGWGVVSEPPTGGERVKAAESALG
                     SGKADLTGTAKGEFNLAADFRPQDWIGVEGTEGASNTLYGLEAGFGITTGVNNSFFGR
                     QAGYADTSGYANTSVGRAAGKLNKTGWENTYIGFQAGNKSTADENVFVGARAGLENLS
                     GSDNCFVGHEAGFKNVSGQGNSFFGFQSGYGNDGSWNTVVGAYAGYAITRGMDNVFIG
                     GSSGEQNSGNDNVFIGKETGRLNNSGYGNVFIGNCAGYNELGANKLYIENTPSTTPLI
                     YGDFSSDKLRFNGAVGIKKTPSYLLEVGTNGAFCNGGVWVDGSSREFKENIETLPAGE
                     AEAAFDKLEPVKFQYRESDGETYIGFIAEDVPELVAMNDRKGLNAMDLVAVLTKVVQE
                     QRSALREQHREILALREDIAKLKEDIRREK"
     gene            55347..>55537
                     /locus_tag="PKV64_02130"
     CDS             55347..>55537
                     /locus_tag="PKV64_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HPN15910.1"
                     /translation="MAQTHYKALFQKALLQFITEPDPFLAMLQWVMTEMMRIEAEAKV
                     GAVKGKHTNERTTYFSGARV"
BASE COUNT        10344 a        18164 c        17582 g         9447 t
ORIGIN      
        1 cccccgccgt ccatatcgaa tctcccagcc aggtttctcg gggggtcaaa ccggcctcga
       61 tctgtttttg aaggtcgatg atgtcccggg ccatgttcag gcgttcataa ttgcggcgta
      121 acttgatccg cacgaccgcc gcctcggctc cccattcgag agccttctca taataggcgt
      181 ttgattcata caggtcgtcg agcctttgac aggcgtcgcc ggccagctcg tacatccggc
      241 cgatccaagg gcgccgctcg tcgggaacga ccaaaaggtt ttgccggagg gtctgcccgg
      301 tctggacgac ctcccggaag cggttctgct taaaggccaa ctccatcgaa acgcccagtc
      361 gctcgatatc gagcagcccc gaccgggacc ggtcctcgac catctcccgg gatttgaaca
      421 gggattccag ctcggcggcc gcgccgacgc cgtcctcggc atccagggcc ttccgaaaat
      481 catcgtgaac cgcccgcaga tccgccccgg ccgccagccg gctcttcagg attccgagat
      541 ggaccgcctt cgccagcttg ccgtattcct cggccggaac gggctccgtt ttcggcgaga
      601 gccgctggta gaaccggatg ccttcgagaa gcgtccgggc ggcccggtag tgctggatcg
      661 attcgtccgg cctcggcgca tgggaggcga tgaggccggc ctgggcttcc cggacggccc
      721 tccggaagtc gaggtgctcg gcctcggcca ggaagccgag gcgcgccgcc gggaagatcc
      781 cctttccccc caggaatccg gccgcccggc gcaaaacgtc cgattccggg ggaaggatct
      841 cttccaggac cgccgtgtcc cggacgttga tttcccaaag gctgagaata agatcgagcc
      901 gctcctcccg ccccggcccc ccggcggaaa gaagcgaccg ggtgatgtcc tcgacgaacc
      961 ggcgttcccc gggggaaagg gagggccacc gagccaagag gatttttccc acctcgaaat
     1021 tgatcaacgt gtccacgccc gaaagggcgg ccgccttgcg gtattcgtcg aagtagcgct
     1081 cggccgccgg gaaatcgagg gcgttgagat actggagcag ctgaccgaaa tcgaaatggg
     1141 ccgtcgggga aaagggatgg aggtcgagcg aacgaacata atttcggtag gcgagacgga
     1201 agtcggcatc ccgctcttcg acctcgccca ggcggccgac gccgctttta aaaagggcgt
     1261 tggcccgggc gtgatacaac ccgtcgttca gccaggggaa gcggcctccg gtttccccgg
     1321 cggccgttcc ctcccccaag gccgtgttcc tcgcccggcc ggccgctctc tcgccgaggt
     1381 aaacgtacag gcagagcgcg gccgccagca ggaggccgaa tccccgtcca aggcgcttga
     1441 tcatgtcagg ttttccggtg ataggccgtc ttcatcgtca gggcgaggat gacggaaaag
     1501 gtcagggcgt tggccgggat ctggaggttg aaatccgtca tgctgtgcgt cagaatggcc
     1561 gccgcggagg ccaggccgcc gagagccagc cccttgagct cgggatgacg ccgggtcatc
     1621 cacatccgga aggtcttgaa caggagcaag cccgcgacga aggccagaag gccgaagccg
     1681 aggacgccca gctcgctgag gaattccagg taatcattgt gggcgtggac gagcgcatac
     1741 tccatgcccg tcgtgtcgta ggcttggtag acgccgccga acgcgccgaa gccgacaccg
     1801 aagaggggga actgaccgat catcgtcatg accccccccc agtagcgggg ccggccgtcc
     1861 tgtaaaagct tgtccagggc gaatcgcccg atcatggttt ccaggccgac atacaggccg
     1921 aagccgagga ccgccgcggc cgcgaccccg atgacgcggc ggacccaggc ttggtggacc
     1981 aagacatgac cgaaatattg ggccgtcaag accagcatca ggccgaagga gaacagcagg
     2041 atgacgaatc ccgagcggga attggagagg aagagggcgg cggccatgac cacgaggccg
     2101 atcccgagaa aaagattcaa gcccagcccc cggttgagga accgggcgaa ccgcgtccgg
     2161 aacggcgttc cgggctcgcc gaagagatcg agacgcgaga cgatcagccc caaggccagg
     2221 gggatcatca tttcgagata cccggcgtaa tggtttcggt tgacgaacgt cccggtggcc
     2281 gaatccaggt tgtgctcctt cgcgtagaac aggagccggg gatggctccg gtaaagctcg
     2341 aacagcccgt acaacgcctc gaacacgccg accccgacca gcgtcaccat gatccggcgg
     2401 atctgtttcc ggtgggtcac ggttcgaacg accaggacgc cgatcaggat gtacgccagc
     2461 aattccaggg acgcggccag ggtgtaaccc ggcaggagcg aaagcgtccc catccccgtc
     2521 cgggccgaga ccgggacgta ttcggcccgc agggcggccg ttcggggcga gaggatccgg
     2581 acgaccgcgt ccggcagggg gagcatttga aaggccagga aaatccccaa gcccagaaac
     2641 gccggccgca ggggacgcat gtccgaaatc agcttggggc tcgcgcgggg cttgcgctcg
     2701 aggctcaggt agacggcgaa cagggcgaag gcggcgattt caatgaccag gaccgaccac
     2761 ggcgcgaccg aggcgatggg cagcggcgcc caggccagaa gagccaggag cccgacttcg
     2821 agaatcctgc gtccgcggcg gtcccggaga ctgaggccgt tttggtcgta ctcgtcgggc
     2881 ccggcttccc gaggctctcc ccggaagccg aatctccgga ttcttacgat ccacatcggg
     2941 cgcgtcagtc ctttttctcc gtctttttct gacggacgac ctcatccacg cccgcgtcct
     3001 ggtagtactc gtactggaaa tacggggtca tgaaatcgcc cttcttcatc tcggcgtcgt
     3061 tgaaaatcac gccggcgatc cgggccttgg ccttggccag ctggtcgatc gcccgcagaa
     3121 gcggcttccg ggtcgtcttc ccggttttca ggacgagaat cgtcatgtcg gccatggccg
     3181 cgacgatgac cggatcgacg acggccagga ccgggggaag gtcgaccagg atgatctcgt
     3241 agctttcgcg gacgatggac agaagctctt tcattttttt ggaattcaga agctcggccg
     3301 gattcggcgg atgaggaccg ctcgggagga gggagaaatg ggcgatcggc gttttctgga
     3361 cggtttcggc cagatcgacc cggccggtca aaacgtcgct caggccgacg gcgttccgga
     3421 cctggaagat cttgtggagg cggggcttgc gcaggtcggc gtcgacggcc aggacgcgtt
     3481 cgcccatctg ggcgaacgag atcgcgatgt tggcgatggt cgccgacttg ccctcctgcg
     3541 gctgggtgct ggtgaaagcg atcacccgct ggtcgctgtc gatcctcgag aaaagaatcg
     3601 acgtccggac gttccgataa tcctcggcga tcgagatttt cgggaagaga tggttgatga
     3661 gctcgatctc cgaaatccgg gccgcctcga ggctttggga tccggcgtct ccgtaggctg
     3721 cggcataaac ccccttgccg ccggtgtccc ggggcgaaaa atgggggatc aagcccagcg
     3781 agggaagccc ggtcagcttg tccagatcct ccggattctt gatcgtgttg tcgaggaaat
     3841 gggcggcgaa ggccaagccg acgcccagca ggagtcccag gagaaaggcg acgatgaggt
     3901 tgcgcttgac gttcggcgac gacggctcgt cggggacgag ggcccggtcg acgatcttga
     3961 tgttgctcgt cttgaggccg ctcagcatgg tcgagacttg ggtcgtcttc tgctgggcgg
     4021 tgagggtcga gatcagggtt cggaggctct cgacctcggt gttcaagccg gcatagaaaa
     4081 gggagtcgtt gttctgccgg gtcacttcgg accgcttgga ttccagcatg tcccgcagcc
     4141 ggttctcgtt gccgagggcc tgggaatact cggagtaggc caagtcgacg gctttattga
     4201 tttccgtctt caactggctt tcgagcgtgt cgaccttgac cttgagctgg atcatctccg
     4261 gatggctggg cgtgtacgtg gtcgcgatct tttcctcgta ctccgacttc gcctggtaat
     4321 aattcgtccg gagggcttgg atcgtcggat cgctgacgtt ctggggaagg gaatccaccc
     4381 ggagcttttg gagctcctgg tacttggcct gggcggcgat ccgggcgacc tgggcgctgg
     4441 aataggcgcc ggccgccgag gcgtattggg aggccgtgtt ggaatccttg tccgacagcg
     4501 gcaggatttt cttctcctcg gaataccgct gcagctggcg ttccttggcg tccaggtcct
     4561 gccggagctg ggcgatggtc tcggtgagga aatccgacgt ctgctgggtc gcttcggagc
     4621 gcgactcgat cgaataatcg atgtactcct ggaccagggt gttgacgacg tcggccgaaa
     4681 gccgcgcctg ggtggagaca tagctcacgt cgaccagccg ggtttcgggg acggggtaga
     4741 ccgacaagcg ctcgagcagg cgcccggcgt acaaggcgtc cgcctcggcg ccggccgttt
     4801 tcccggcccc cggctccgcc ggccggccct tcggccggag ccagcggaac gtcaggatat
     4861 acttggccat gtcggccatc gtccggttct tttccacttc ggccaggaat tccggccggg
     4921 ccccgagatt catcttccgg acgacccgct cggccaagct gcggctctgg atgatccgca
     4981 gctgggtgtt gaaatacgtg cccaggaaat cgtcggtcgt gttcgtcggg gcgttgagat
     5041 attcctggag attgaaccgg ccggagccct ggtcctcgat caggatctgg gcggtcgccg
     5101 tgtaccggcg cgtctgggtg aacgacagga ccgcggccag gaccaggcag gcgccgagcg
     5161 tcgaaaagac gatcgtccgc cgcttccaga cgatggccca gtattcgccg aggttgatgt
     5221 cctgccgatt cacatttcga gcgccggtca tttgatcatt ccttttcgct tccgtccgga
     5281 ggatcgtccg ccccggacca gcctcacctt aactccgggg cgggccgccg gcaatcgtcc
     5341 ggggggatga accccccggt gatatttcac ctctcccctc tccccttcgg gggtgagaaa
     5401 ggcaaggggc ggcgcccccg gacgtctttt aaaaaatcgt gtccggaacg taaaccgtgt
     5461 cgccttcctc gagagggaaa tcctcgagcc ggttgttttg gatccggacg acgttgatct
     5521 cgaaggtttt ttcccggccg ttttcatcct tccgggtgac ggtcacccgc cgcttggccg
     5581 cccgctcggt gaatcccccg gcctgggcga tggcctgggt gagagtggga atccgcgatt
     5641 gaagaaccga cagcgcgccc gggattttca cctgcccgta gatgtagacg tggacggtcc
     5701 ggtcgatctg gacgatgacg ctgtcgccgg cctccagagg gacgttgtat ttcgggtcgc
     5761 cccgcagcat caggtcgtcg acggggatct tgagattgcc cgtcgtcccg tcgggaaacg
     5821 tccggaggat gatgatttcc cgggcggctt cccgggtcag ccccccggcc gcggtgatgg
     5881 cctccagaag cgtcgtccgg ccgaaaagct ggacaaaccc gggattggtc accgccccga
     5941 ggatggacac ttgcttgctc tgacgctcct tgatggtgac ggcgacgtgg ggatcccgga
     6001 gaaaaggccg gtagagctcg gccagcttgt cccccagctc ggcggccgtg aggccctcga
     6061 catagacctc gttgatatag gccaagttga ctttcccctg ctcggagacg cggacctcgg
     6121 tgttcagctt ctcgtcctcg cggaccttga tctccaggag atccttgggg ccgatccggt
     6181 aggcggtcac gaatctctcc gcctgcccgg ccggatcctg ggcggcggcc acgccgatga
     6241 cgataagaaa aagaacgatc caggccgcgg ccgccgtcgg gagtctcatg cgcgtctcct
     6301 cattaaaaat accgggtgta cgtcaagccg acgaaattcc gggcccggtc gaaagacccg
     6361 agcgtcgacg tccagcgctc ggagttccag gcgatcccga ttcccgccgt ccgcagcacg
     6421 cggacggtca agcccagcga atgattccgg cggcggtcca cccggtttcc ggaatcctcg
     6481 cccgccgccc gctcggagta atccgccgtt ccgccggaat aatcatagtc gatttttaaa
     6541 aacgaggtca aatagaggga gccgcccgcc ccccaggaat tctcgacgaa gtagaaaaca
     6601 tcggcgtagg ttgaaaaaac aaggtcccgg gcgaaatcca ggcggaaggc cagccggccg
     6661 gtccgcaggt cgaggccggt gttgccgacc aagccgcgga agggcggctc cccggcccgg
     6721 ttcggattga aattcttgta tccgaggttc aggatccccc ggatccgccc caggagcggg
     6781 aaccggaggc cggcccaaaa cgagaccgct tcggcgtcgc gccaggcggc cgtctcgtcc
     6841 cggaaacggt attcccgcaa gtccccgcgg aggaaaagaa acccgtcggg aaacagctgg
     6901 taataggctt ccgctccgat ctcgcgctcc tcgcggtcaa ggagacggga aaccggcgcg
     6961 tccgccggat cgatacggat ctcctcgtag gatatccggc gaaaggctcc cgtgattccg
     7021 atcgaggttt tcctggcggt ttcataggac aagccggcct cgaggctccg gacccggtcg
     7081 ttggccggaa tctggaattc ggacgacaag cggcgccggt ggacgtcgtt tccgaaggcg
     7141 cccgagagga cgaagcgccg gagcagcagg gaccggaatc cggctccata gctgttggtg
     7201 aagccccgct ggcgcgtttc ccgttggaaa taggcatact cgggattttc ccggaacgtg
     7261 aggatcagga cgtcccgcag cggccagtaa acgcggatct ccggcgacag cgtggccgtg
     7321 aaatcctttt tcggggcgcc gtcgtcgctg ccgaaataga ggttgtcatc gaatcccgcg
     7381 tcgcggacgc cgagggaggg aaaaagccgc agcggcccca ggcgcaaaaa ggcctgctcc
     7441 gtcaggcgcc ggtattcatc ggcaaaaacc tggtaggact gggcggacag ggctccggcc
     7501 aggccgagac agaagaaaat caaaacgggc aagcggcgca tgatgatccg gttcaaaccc
     7561 gtcaattcag cagacggtcc aggaaaacgt tgttccggga aaggcccttg aagatcccgg
     7621 cgacggccgt tttccggaag agcgacagga tgaggccgct ctgccagtcg aattggcctt
     7681 cgttctgaat cacggggatg aggccgtcgg actgggccag ggtgaacagg gcttcgaatt
     7741 gccccgcgct catcatcgaa taaaactttc gggcgtaata tccgatcgag atttcccgcc
     7801 ggaggccggc tttccaccgg cggtcgtagg cggcgagatc ggccggtccg aaggcgttct
     7861 ttttcagcgc ttcgaggatg gtttccgaag ccagccgggc cccccacaat ccgtaataaa
     7921 ttccgccgcc ggtcgtggtc ttgacctggc cggcggcctc gcccacggcc agggcccggt
     7981 cggcgaccga tcttttcgcc atgccctggc aaatcggctt gaactcgagc cgccggacgg
     8041 ctcccgccgg atcgagccgc gggtaatagc gggcggcgaa atcgtcgaag accttgcgcg
     8101 gattcccgtc ggtgatcagg ccgacccgga accggtcccc gcccgagggg acggaccagg
     8161 cgaatccccc gggcgcggtg ggcggcccga cgaaaatcgt cggggcgacg cttccctccc
     8221 cccggatctc gacttgggcg ccgtaaatcc ggcgggccgg cggcgtcaag cccagcttcg
     8281 cgtggagccg gccctcgact cccgtcgcca ggacggtcag ccggaccctc cgccggacgg
     8341 gccggccgtc ccgctcggcc tcgatcgtca ccccgtcgcg ggagaccgag acgtcgcgga
     8401 ccatcgtccc cgtctcgatt tccgccccgg cttcccgggc caggccgccc aggtttccgt
     8461 cgaaacgctc ccggtcgacg atggaagcga acggcgaggg atggcggtag cggatcgagc
     8521 ggccggaaaa ggaaacgatc tcggcttcct ggaaatcggg tccgatggtt tccgcgggca
     8581 ggcggaactc ggaaaagacg tcccggccga tcaagcccgt gcagacgacg ttcgccccga
     8641 cggccggctt cgcctcgagg acggtgacat ccgccccgcc ctcggccagg aagcgggcgg
     8701 tctgcaggcc cgccggcccg ccgccgatca ccgccacatc cgcgttatcc attgttccgc
     8761 cgcctgtttc ggccggatcc tgatcacccg gcctttttaa cttggaaaat cattatcggg
     8821 attttcccct gtgaaatcaa tcggaatcct agattttcga ctgccgggac tcccatttcc
     8881 cccggaatgt gatccagaag gaaagaaaaa tcaggcgcag gtcgaacccg aagctccgct
     8941 tgcggagata aagcaagtcg taccggaact tggcccgccg gggcgtgtcg ccgggaagaa
     9001 agacctgggt caggccggtc agccccgggc ggacgacgtg gcgctcccgg tagcccggga
     9061 tgtcctggat gtccctctgg tcgggatcgc cgcggacctc cttctcgttc ggccggaggg
     9121 cccgcggacc gacgaagctc atgtcgccct tgaaaatgtt gaaaagctgg ggcagctcgt
     9181 ccatggccgt ggcccgcagg atccgcccca cccgggtcac ccgcggatcg tcctcaaacg
     9241 cctgctgggg cccgtccttt tccgcgtccg tccgcatcga ccggaacttc agggcttgga
     9301 aaatccggcc atccttgccg acccggtcct gtttgtaaaa gacaggcccg ccgtcctcga
     9361 tcttgatgag agccgcgatg acggcccaaa gcggcgacga gacgaacagg ccgaagaacg
     9421 acaaggcggc gtcgaacggc cgcttaagga acagagcctt gcgccaagcc gcgctcacgg
     9481 ccgtctcccc cgcggcccgc gggcggcctc ctccatcagg gcttcgaacg cctcgaccac
     9541 gctccggcgc gagtacacgt cctcggccag gcggcgcccg cgggcggcca tcgcctcccg
     9601 gcctcccgga tcatccctca aggccccgac ggcggccgcg atccgttggg gatcatcggg
     9661 cggggcgcaa aggccgcatt ccgcctcgcg gatgattcga tagacctccg aggattcgac
     9721 ggccaagccc aggatcggcc ggcccgcggc caggtagttg taaagcttcg agggaacgga
     9781 gaggaacgac ttctccttgt ccaagggaac caggagaacg tcggccgagg ccagcaagcc
     9841 ggggaggtcg gcgtagggct ggaacggcag gaagacgacg ttgtccagcc gcagctcctc
     9901 ggcccgggct tggagatcgg cccgcttaag gccgtcgccg acgatgacga ggcgcgtctc
     9961 ccggtcgggc gccaggaggc gggccgcctc gaggatccgg gccagcgttt cgaagctgga
    10021 aaggctgatg atcccggaat acatgacggt gaagacgccg tccaggccga ggcggcgggc
    10081 cacgggatta tccttgggca gggggcggag gaagtcagtg tcgacccagt tggggatgat
    10141 cgagatccgc tcgtccggca cgcccttggc ccgcaggttt ttccggaacc cgtcgctgat
    10201 gacgacgatc cgccggtggc cgtcataaat ccatttttcg aatttcaagg cggcccggat
    10261 cgccagggga tggcggagga tgccggcttc aatcgacagg tccggatgga tgtcctggac
    10321 gttgaggatg accgcggccc cgcaaaggag ccgggccagg attcccgtca gcccgagctg
    10381 cagcggcggg agccggagga aaacgacgtc gccgcgccgg acggtccgga ggttccagat
    10441 cgcggccagg gtgtagatcg tccaggcgat cagccgtccg gccggatggg acgtgacatt
    10501 ggtcgggtag cgtttgacga gaacgccgtt ccgcgtctcc cggacgaaca accgcccctc
    10561 gtagccgcgg ttgtgaggcg gccgcgggag cgtcgtgacg atctcgacgc ggtggccgcg
    10621 ggcggccagg gaggaagcca gctcgttcat caggatgggg gcgctcccca tctccggaag
    10681 gaagtacatc gtgtggatca ggatgcgcat gccggggcgg cctcctcgcg gggggattca
    10741 tcgccgggag cggtccaagc ggccagggcg acgcaggcga tcgccgggat gaacaggaga
    10801 agatcgacgc cgtagaaggc ggccgtcgat tcgaagaagc tgcgggcggt caggaagccg
    10861 gcgatcatcg tggattcgaa gagcagcgtc cggcgctccc cggtgaaccg tttcgcccgg
    10921 aaaaggattc ccgaccgggc gaccattccc caaaacgaga ccagggcggc gacgaacaga
    10981 agcatgccga tcacgcccgt gtggatggcg gcgtgaagat aggaattgtg catgtgctcg
    11041 gaattcatca tgatccggtc ggcctggaag ccccagccta aaaagggcga tttcttgata
    11101 agctccaagc cggcccgcca ggtgtattcc cggccggtca ggctcatcag ccggtccatg
    11161 cttccatggg accgccactg gaatccgctg atgtacacga cggccacggc caccgggccg
    11221 acgaacagga accgccagtc gcttccccgc atgatgacaa agaggacgga ggccacggcc
    11281 aggccgagga gggccgtccg cgactgggtc tgggtcagga ggaagaggaa cggcagggcc
    11341 gccgggatca gaagatagcg caggcgggac cggccgagga ggaaccggac cgcgaggacg
    11401 atgatgatgg tcagggcgta gcgcccggcc ccgttgacgt tgatctggcc gaggccgaac
    11461 atcagggtgt actgcctgaa cctcggcaga ttgccccacc ccacccggaa agcctcggga
    11521 aggatgctga cggcgaccag ggcgaacatc gcgtaattgg agtagatcag ggcccggacg
    11581 gcgtcccagg gatcgggacg ctcgagggcg aaccaggcgg ccatcagcgg ggccaggtaa
    11641 ttgccggccc aataaatcgc cgtcaacggc tcctgcgaca ggaacagcga cgacaggacg
    11701 ccgacggccg cgtaggcgta ccagaacagg agcggattcc ggcggaacgg aaaggccggc
    11761 cgctccttca ggatccagag gaggcagaag aacagggcgg ccagcggaaa cagcgaccgg
    11821 atcccctgga ggaaacccat ccccgaccgg aggaacgatt cgcttccgac ggcgtcgatg
    11881 ctggtgaaca tcccgcccca aaccagaagc cagagaaaga cgctggacat catgtccgtt
    11941 ccggtcccag ggggcccgcg acggcttcgg cctgaatgtc ccgaagccgc tctcccaggc
    12001 ggtcccacag ataaaaatcc tccgcccgcc gacgggccgc ccggcccagc cggagacgga
    12061 ggccggggtc cttccagaga cgttccaagg ccgcggccaa atcctcgacg acctgttccg
    12121 gattccgcgg ttccaccttg aggccccatt ccggctggat gtggaatccg gggccggccg
    12181 aatcgagggc cacgacgggc ttgccgcagg ccatcgcctc gatgacgacg atgccgcctc
    12241 cttccctcaa gctgggaaac agaaagacat cgcacgccgt catccgggtg atcagttccc
    12301 gccgcggcat ccaggggacg aaccggatgt gttcctccat tttcatctcc cggaccaacg
    12361 cttggagggc cggtccttcc ggcccctcgc cgacgatttc caggagaacg tccgccccgg
    12421 gattgttccg gagaaaggcc gccgtcgacc ggatgcagaa atcgaaccgc ttgtagcgga
    12481 ccaagcgccc ggtcgtcaag agccggaaag agcgatcccc ctccggccgg gcgacggggt
    12541 catggagatc cgcccggttg atgccggtga ccgggaagaa acgaaccttg gcccggtcgg
    12601 ccggcggaat tttccgccgc gtttcctcgt tgcaaaccag gatcgcccgg gcccgggagg
    12661 cgcaacgccg ccggccccgg atcaggcgcc ggccgagctc ctgggagagg atccggtagc
    12721 gctcccccgc ccgggctttc cagccgtatt ccgactcgaa gcccttgggg ttgcgctgcc
    12781 ctccgccgat cggaccccag acgaagggaa tgtcgaggcg ggctccgatg taatccggca
    12841 tccaatcatt ggcgaacgtc gcgtgctggg ccaggtcgaa agcgaccgtc cgccggagct
    12901 tccgggcttc ggcccaggcc agccactgcc agaaataata ataaaaatgc agggtgaagg
    12961 atttatgcca gagcggcttc caccagagct tgtaatcgag atagcggaag cggaccccgg
    13021 ccgcctcgcc gcgcatttgg gcccgctcga tccccggccg gttccgggct tgcgtcagga
    13081 cccaggcgtc gtggaaccgg gcgacctggt tgacgatgtt ccagccgagg atggattcac
    13141 cggaggcgag gagcttctcc tcctcgggcg cgcccgcttc caagggcggg tggcaggcga
    13201 aggcggagat cagaacattc aagcgtttca tgggcatcct tatttcatcc gcgagaacag
    13261 gcgggacgcg cgcccggcct tgaagcggga gacgaacgag gccgcccggg gcgccttgag
    13321 gaccgtccaa agcttcagcg tccgccccaa cggcagccgg aggccgagga tgtccccggt
    13381 cgaggccccc ggccgggccg acacccggta gtatttccgc atctcggcca ggcgctctcc
    13441 gaacgccggc acgtccgccc cgaaaaaagc ggaattcttc tccaccgcct ccaagacgag
    13501 aagcatcgcc tcggcccggc ggcggacgat gtccggggaa aaattgaaaa ggccgccgaa
    13561 gatctggccg gcccgctggc ggtagagata gagcggctcg tcgaaatgaa cgatccgggc
    13621 ggccgctttc ttgatgacga gggccaccca gaaatcctcg tgaatggagg gaagatccgg
    13681 catgggaaat aggacgtccg cgatccggcg ggaaaacgtc caggcccagc gcggcgggct
    13741 gacgatcccg ccgcggaaaa cctcggcgta gggctggacg gcgaagcggt ccgtcaaacg
    13801 gagcaggctg aggggggcga gcttctcgtc gacgatccgg gcatcgtggg tcgaggcgtc
    13861 ctcggccagg atctcgggca gacgggccga aaatcccgga tcgagggcgt cgtcgccgtc
    13921 gagaaacttc acgtggccgc cggaggacgc ggcgtaggcc gcgttcaggc cggcgaccgt
    13981 cccccggccc ggattatcga tgacgatgat ccgggaatcc ccgccggcca gccgcgaggc
    14041 caggtccttc gtcccgtcgc cggaatggtc gtcggcgacg acgatctccc agtcggccga
    14101 ggaaagggac cggacgccgt ccaccgcatc ggccagccag ggcgcgacgt ttttcaccgg
    14161 aatgaggaag gacaggcggg gtttcaccgg ccgctctcct ttttcgaggc gacgagggcg
    14221 gcgcaggcgg ccgcgaccgg ggcgaccagg ccgggccagt cgaagaggcg gcggacttcg
    14281 gccgcgccgc gggcggccat ggcctcggct tcgccgcgat ccgcgtaaac gccgttgatc
    14341 gccgcggcga aggcgtcccc gccgtcggct aaacggagat ggacgcggtc cttgagagga
    14401 agcccctcgg cgcctttggc cgtcgcgacg acggggattc cggcggccgc gtattccaag
    14461 atcttgatcc gcgttcccga gcccgagaag acgggggcca ggcccacggc ggagcttcgg
    14521 atgacggccg ggacatcggc gtggggaatc atcccggggt tgaccatcca ggggggggcg
    14581 gatccgcgga ccgttccgcc gaggacggcc agccggacgc ccgggttggc ccggacgacg
    14641 ttttcaaaaa tgccgaaaag gaaccgcagc gcctcgtcgt tggggggata gcccagaaga
    14701 cccatgaaaa gggcttcccg tccgttcaag ccgtagcggg cccggacggc ctcgatctcg
    14761 gccggggaaa tccgctcgaa cgcggccgcg tcgacgccgt tgggaagaag acggggctga
    14821 acgccgtaga ggcgggccat ggcgccggcg tcgatttcgg aaacggccgt ggcgatgtcg
    14881 acggaccgga ggagacgccg ctccccccat ttcgtcaagc ccgagacgac ggcccgatcc
    14941 ttgcgctcgc ggcggagaat ccattccacg ttgtgggcgt ggtaaacgaa gacagcccgc
    15001 ggacaacggc ggcggatcaa gcgatgaaga acggcataat acggcgccca ggacgagcct
    15061 tccatcacca cggcgtcggg ccgttccgag gcgacccggc gggcgatccg ggaaaaccgt
    15121 gccagcaggc cgagaagctt gcgcagccgc cgggtcgatg aaagccggaa gccgacgagc
    15181 cgaaggccgt ccggcgtcgt ctccggcgtc cggccgtcgt cgagctgaaa caagacccgc
    15241 tctccgggcc aggcccgggc cgttcggtaa gagaccgcgg ccgcgcccga aaagggagga
    15301 tacgcctggt aaagcgaaag gaaaacggtt ttcatgagcc ggcggcctcc ccggccgcct
    15361 tgccccggaa aaagcggcgt gccaggccgg tccatccgtc ccgttcctcg ggagtgaggg
    15421 cgcgccgcca agcccaaaga acgtacaaag cggacgccag gacgatccaa agaggcgcgg
    15481 ccggaagccg cttcgctccg aaaagcgcgg ccgtaaagac cgccaagatc agcccgattc
    15541 gaaagatccc cgtctcggcc aaggcgcgga gggggatttt cccgacccgg agcgaagcct
    15601 cgataagcag gccgaggtcg agcagcaccc ggatcgtgca ggcggccgcc gccccgttga
    15661 tcccgaaccg gagaacgccg aaccacaaca gcgggatgaa gacggccgtt tcgatcaact
    15721 gaaacttggt cgggaggtcg gcccggccca agccctgcag atagctgtac gggacatacg
    15781 ccagggcgga aatcaggaag cccagggaca ggatctgcag gacgagccgg ctttggaggg
    15841 cgaattcagg cccaagccag atcctcagga tgaatccggc gagggcgatc aagccggcgg
    15901 ccggcggccc caggagaatc aaggtgaact tcagggaccg ggtgaaaagc cgcttcgagc
    15961 gctcggcgtc gccgccaccc tcgagagcgc tgaacgccgg gaacagcgtc agggccaagc
    16021 ttccgggcag catgttcatc cgtccggtcg cctcggccgg ggcggcgtag tatccgacgg
    16081 cggtcatgcc gcgcagcgtc ccgaggacga accggtcggc cgagacgagg aagaaccaga
    16141 cgaagctcga cagcgcgttc cagccgccga agctgaacag cttccggagg cggtgtctcg
    16201 gaagaaagcc ctccttccgg gccaggaccg ggtaaacgcg gaaagcgagg ccgatccaga
    16261 taaacagcgt cacgacgcgc gacgccgtca ggagaaggac gatgccccgg agccccatgc
    16321 ccagcggcgc cccgatcagc gggagaacat agatcagggc gttggtcgga atcttaaccg
    16381 cgttgacaag ctcgaagcgc tggccggcct cgagcacgcc ccggatggac ggggtgacga
    16441 tcgcgaaggg aagcgagagg gccatgatca tgagcgcgcc cttggcctcg gccaggttcg
    16501 ccgccggaat cttgaggatc cgctcggcca ggagcggagt caggacgatc atcaggacca
    16561 tgccggccag gccgaagacg gtctgcaggc cgaccgtggt ccggataatc cggggcaaat
    16621 cctccatcgc cccccggccc atcgcctcgg ccgagaactt gatggacgcc cggcccaagc
    16681 ccaggtcgaa caaggcgaaa tagccgaaga cggtcaggat gatcgagaaa atcccgaacc
    16741 ggtccgtccc cagccagcgg atcacgacgg gaacggtcac caggccgatc atcgccggga
    16801 cgacgattcc caggagattg aggccggtgt ttttcaccag ccgccgggat ccgagatccg
    16861 gcttggcgtc ccgggccggt tcgttgtgaa ggctcatgtc gaggctttga acaggctgcc
    16921 gccgatgggg gaatttccgc tttcccctcc gtccgaaatc atcgttttcg gatacactgc
    16981 gaggccattc cgctcaacgt ttttcagagt ggaccggaag ggctgttttg acaatcgtcc
    17041 ggccggatga attccggggt gacatttccc cctcccctct cccctcgaga ggtgagaggc
    17101 gctccgaaaa ccgcccgccc gcgtcctcct tttctttgac aatcctccgc tccggcggta
    17161 taaaatatgc tgttctttca tgttcatttt aaaggacgga cagggaaacg ccggtccccg
    17221 gccggggaat cggagcctcc tgcttttcgg tctcggattt ctcttcctcc tggccgccgt
    17281 ccctggcttc cctctctcgc aagagacgga tccattctac ctcaaatggc tcgaatcggg
    17341 agaggcggct tatcttgccc gcgattaccg acaggccgtc tcgtatctcg aaatcgccgt
    17401 cttcggcctg gcccgggaca aggatctcca ggccagggga tatgtcttcc tggctctctc
    17461 gcggacggct ctcggattca aggaccgggc ggccgccgat ctttcggccg ccgacggtct
    17521 cgtcggccgg gacggcctcc ggtcgatgaa tcttccggag gcggcccgga tgcggctcga
    17581 cgccttcctc gcctccgccc cggcctcgaa ggctccgccg gaaacgccga cggccgccgt
    17641 gacggcggaa accggcaccg ttcccccgcc gccgccttct gaaatccttc cgtccggcga
    17701 ccggacggaa gcgacgccgc ttccccccgc cgagacgcct tcgactgaag atctccgccg
    17761 ggcgatggag gcgaatcccc gggagccgcg ttcctattac gagctttccc ggctgaaccg
    17821 gcgggccgga aaaaccgagg aggcccggga aatcctcgaa acgcttctcg cccgcaatcc
    17881 ggccgaaatc cgggcgtatc tcgaaatcgg ccgcctcgat tacgagaaag acgatttcaa
    17941 agcggcgatg aaagccttgg aaaaattcat ctccttgtcc gccggcgttt tcgtcgagga
    18001 acgattccgg gacgagggcc gggctcttct cctcttgagc gcctcgcgcc ggggcgaggc
    18061 gaggaaaacc gcccgcctgc tcgaggaatc ggaggaatta ttccgcccgg aacgattcgc
    18121 caaactgact ctggacccgg ccgatttgga gaggctgcgc cgcctccagc ggacgtccgc
    18181 cggctgaggg ggcgcggcgg ccggttgaat tcgtccctcc gaccgggtag actaagcctc
    18241 cgggaaacat catgacacaa atcgaatccg cgaaaaaagg ccgggtcacg gcggaggcga
    18301 gggccgtcgc ccggctcgaa ggcgttccgg tcgaatcgct tatggaatcc ctcgccgccg
    18361 ggaccgtcgt catcccccac aatcgcctcc accggccgtt ccggccggcc ggcatcggcc
    18421 gcggcctccg gaccaaggtc aacgtgaacc tcggcacgtc ccgggacttt ccccatttgc
    18481 cggccgagct tcaaaaaacc cggatcagct taaagtacgg aacggatgcc ttgatggacc
    18541 tcagcaccgg aggcgacata cggaaaatcc gccgggcgat tctcaaggcc gccccgatcc
    18601 ccctgggcac ggtcccgatc taccaggccg cgatcgaggc catcgcccgc cgcggcacga
    18661 tcgtcgacat gaccgtcgac gaccttttcg cggccatcga ggcccaagcc cgagagggcg
    18721 tggacttcat gaccgtccat tgcggcctga cgctccgggc gatcgaccgc ctccggggac
    18781 agggacggat cgccgacatc gtctcccgcg gcggcgcgtt ccacgtcgcc tggtgtctcc
    18841 atcacggcaa ggaaaatccg ctcttcgagc atttcgaccg tctcctcgaa atcgcccggc
    18901 gccacgacat caccctcagc ctcggcgacg gcctccgtcc cggatcgatc gccgacgcca
    18961 cggaccgggc ccagatcgag gagcttttga cgctgggcgg gctcgtccgg cgggcccgcc
    19021 gggccggcgt tcaagtcatg gtcgagggcc ccggacatgt ccctctcgac caggtcgaaa
    19081 tgaacgtcaa gctggaaaag cgcctgtgcg agggcgcccc gttctacgtt ctcggtccgc
    19141 tggtgaccga catcgccgcc ggctacgacc atatcgccgg ggccatcggc ggggcgatcg
    19201 cggccgccgc cggggcggat ttcctctgct atgtcacgcc ggccgagcac ctgggcctcc
    19261 cctcgcccga ggacgtgcgg gacggcttga tcgcctccaa gatcgcggcc catgccgccg
    19321 acctggtcaa gggcgttccc ggcgcccggg agcgggatga gcggatgtcc cgggcccgga
    19381 aaaagctgga ctggaagacg cagggaagat gggccgtcga tccggaaaag ttccggagcg
    19441 tccgggcggc ccggaggacg cattcccccg cctgctcgat gtgcggggaa ttctgcgcga
    19501 tgcggatcgt ttcggaatat ttgggagggc ggagagcggc cgatgactcc cgctgttagg
    19561 gggcttcatt tcggaaccgc cggcgtccct gattcgactc cccaggcctc gaccctggcc
    19621 gcgctccccc acttgaaatc catcgggctg gacgccctgg agatcgaatt cgtccgggga
    19681 gtgaaaatcg gatcggacac ggcggccaag gtccgccgac gggccgagga gctcggcatc
    19741 ctcctcagcg cccacgcgcc gtattacgtc aatctcaatt cccgggagat ggggacccgg
    19801 cttcaaagcc gcgagcatct gctccgctcg gcccggatgg gcgccctctg cggctgccgg
    19861 agcgtcgttt tccacgccgg ctattacggg agcgcgacgc ccgagcagac gttcgacgtt
    19921 ttgcgggagg ccctgcggga aatcctctcc gtcctgaaat ccgagcgcaa ccccgtcacc
    19981 ctccggatcg aaaccatggg ccggcggtcc cagttcggct cgctcgacga ggtggtctcc
    20041 ctctgccgcg acgtcgaggg gctggccccg tgtttggatt tttcgcatct tcacgcccgg
    20101 gagggcaagg ccaacagcta catcgagttt caccggatcc tgagaaaggt cgaacggcgc
    20161 ctcggccgcc aggccctccg cgaccttcac gtgcatctct ccgggatcgc ttacaacgac
    20221 aagggcgaga tcaagcatct caacctggcc gacgcggatt tccggtacga ggaatggatc
    20281 gccgcggtca acgacatgaa cgccgagggc ctggtcatct gcgaaagccc ggcccgcgaa
    20341 cacgacgccg ccgtcctcaa gcggctttac gaaaccaagc gcctgaaatc ctaagccgcg
    20401 gccggaacga ggggatcatc agcgggcggc gtccggatgc cgctcgagcc aggcggaaaa
    20461 cgatccggag aattccgggg cggcggccgc ggccgccgcg tccaggaggc ggaaaagacc
    20521 ggccagcccc tccaccggct tccgctcccc gctcaacagc aggtactccg gcagaccggc
    20581 gagaggcgac ggcgtccggg cggccgtcac ccggccgtcg cggatttcac agccgcagat
    20641 ccattcgatg aaggcccgcc attccagcgg ctggacgtcg ccgccgaaga gcccctcggg
    20701 ccgggtccgg ggactgagca gctcgaccga ggcgacgacg gggcggtccg ggtccgggat
    20761 catgtccggt gaaagatcct tgaaccggta aaaggaaaag agaacgggcc cctcctcgat
    20821 caccgcggtc cggttgaaat aatagcggag gttctcctcc agccaaaagt cggcgaaaac
    20881 cgtcgcctcg gccgggacgt ttttttcaat gaggccggcc cgggaataat cgacgccgcg
    20941 cgaggcgtgg gccggcaaaa cggccgcggt caggttgaaa agaaaaacga aaaggacgac
    21001 ggccgccgcc ccggacaaaa tcaggcgccg ggccttcccg ccgccggcgg cctcccagcc
    21061 gtcggccgaa atcaaggcca aaatcaagaa ggccggcaaa agaaggggaa tggagagctc
    21121 atatccggcc gggctgaacc accacatgaa cagggccgtg cccaagatcc agagaaccag
    21181 ggcgatccgc gcgttcgccg ccggcttgcg ccgggcgatc gaccggagca ccggcacggc
    21241 cagtccggcg cagagcacgc ccgagacaat cgaggccggg accagcaaga agcgggggat
    21301 gaagagaacg tcccgggcga agctgagaaa gagcttgcac aggccgagga gggacacgtt
    21361 ggagaaggag ccccaggtgg gatcggggtt gaaggcatag gacaggcacc agcggacgaa
    21421 tccctccgaa gccgccggac gggccgccga taaaaacacg gccgcgtaag cgcccaggac
    21481 gatcgccccg gcggccgtga tcaggcggcc ggtccgccgc gcctcccggg cgctgaaccg
    21541 cggcgtcagg agaaccgcca aggccaaaac gaaaaagacc gccatctggt tgtaaaggac
    21601 ggccaggacg aaaaagccgg ttgccgccgt ccgcgcgccc gggcccggcg ggccggggcg
    21661 cggcgaaagg agaaaaacga ggctgacggc caggcagccc agggacggga tgtaaatctc
    21721 gacgaaggtc gcgtactgcc agaaaccgag ggccgagaaa agggccaggg aaaaaacggc
    21781 ggccgcgccg gtcgagccgg tcagccggcg gacaatcgtg aaaaaagcgg ccagggcggc
    21841 cagggcccag agaatgttgt ggacctggcc ggcggcgatc ggatcaatcg agggaaagac
    21901 ggatcgaagg cccagccaga atccgcggac gacggcgttg aacaaaagat gatgcggatg
    21961 aaagaggttc gtccccgaac ggaccgcccg ggcgtattcg aggctgtctc cggtctgggt
    22021 cgcggccggg aaaacgatca ggacggccgc ggcgaagacg atgaccgcgg ccgcggcgga
    22081 caaggccccg gccggcgaat cggatgaacg gcgcaatccc gcttttcctc cttgtcgctt
    22141 ttccccgcga taccctcccg aaaaaactat cacaacctcg aggcggaatc aatcggccgc
    22201 cgggatctgt cgcgtctcaa ggcgagggat ggaccggccc gttcagaggg gaaaccccgc
    22261 ttgccgccgg gatcgtcttg ccctatattg aaaaaggaag ggagagaaac atgaagcgca
    22321 agctcgccgc cgccgctctc gtcctgctgg tcgtcctcct tctccctgcc cagcagaagc
    22381 gcatctacat cgccaacgac gaccacaccg attacctctg ggcggccgac gcgaatacgt
    22441 atcgcgacgc cttcctggcc atgctcgact attacctgga ccaggccgag gcgaccgccg
    22501 ccgatccgga gccctaccgg aaccggttca acgtcgacgg gacgttttgg atccggactt
    22561 atgaaaacaa ccgttcgccg gccgaattcg accgtttggt cctgcggatc aaggacggct
    22621 cgatcggcgt tcccataacc ctcctcaacc tctgctacgg ggccatgccg gccgaagcgg
    22681 tcctgcggag catgtattac gccgggcggc ttgagcggag atacgggctc cgcttgaagc
    22741 tcgccctctc ccaggaaaac caaacccttc ccctcggcct gggaacgctt ttctcgggag
    22801 ccggggcgtc ctttttctgg aaaggcatct gcggctgcgc ctcgcgcgtc cccgacgcct
    22861 gggaccggcc gctcgacatg tattggtgga ccgggctgga tgggagccgg atcctgacga
    22921 aatggaactc catgatccgg ggaaattatt acgacctcgg cggctacgcc gaagcccgga
    22981 acctgcccga agccgtttcc ttcgtcacct ccgacgcccg tttcaaagcc cgccacccct
    23041 acgacgtcat cggcattttc ggctacggcg gggacgacct taaatccttc accgatcagt
    23101 tcccggccgc ggccgagacc ttgaccacgg ccgaccgccg gatcatcgtg tccaacatca
    23161 acgatttttt cgaggatttc gccgccgccc acggctccgg cctcccggcc caaaccgccg
    23221 gcttcgggaa cgaatgggac atcctgagcg cctccctggc cgaggtttcc gggcgggtca
    23281 agcgggcgac ggaaaaactc cggacggccg aggcgatggc cgccatggtc agcctccagg
    23341 attcctcgtt catgcgcggc cgggagggcg cgggggaatc ggcttggttc gacctgggct
    23401 tgtattacga ccatgactgg acggccgacg ggccgatcac ccgcgaccag cgggccgcct
    23461 ggcaaagaga gcgggcggcc ggaatcgaat cctacgtcga cacgctggac aaagacgccc
    23521 ggtccgcctt gggcggaatg atcgaaaaaa gcgggtccaa ccctcggtac ttcgtcttca
    23581 atcccctcgg ctggacccgg acgggggaag cggacctgcc ggccggcgcc gcggaacaat
    23641 cggtccgggt gatcgatacg tccaccgggc tggaagcgcc gtcccagcgc gtcgtcctcg
    23701 acggccgggc ttatctccgc gtctggacgg aaaacgtccc ccccgtcgga tacaaaacgt
    23761 atgaaatcgc gcccgggacg gccgcggttt tcgacggccg tccgacggct ggaggaaacg
    23821 tcctggaaaa cgatcgttac cggctggttc tggggaccaa cggcgcgatt ctcagcttga
    23881 tcgacaagcg gcgcggcgga agagaaatgg ttcggccggc cgacggacgg atgatcaacg
    23941 accccggccc cggctccggg acgatcgagg ccgaaaacac cggcaacgtc tccgccagcc
    24001 tgcggatcaa atcctcaagc cccctggtcc acgaaaccgt cgtcaccctc taccgcgact
    24061 cctcccggat cgacatccgg aacgaaatcc gccagaactt cagcggtctc cagacatggg
    24121 atttcaacgt caatctcgac gatcccctgc tccggcacga ggagatcggg gcggttctcc
    24181 tggcccgccg gacggtcgac ggcgggcatt actcgcccaa ggccgcccgg accgattggc
    24241 tcaccctcaa tcatttcgcc gacctgtccg ccggagagac cggcttgacc gtgtcgagcc
    24301 ccgacctttc gttcgtccgc cgcgggacca gcacgccgac gtttctcgac acgcggacgc
    24361 cgcggatctc catcctggcc ggaggccaag tggacgggcc cggcctcggc atccccagcc
    24421 agggcggcga ctcctttttc ctccagcgct tcgccctgga gacccacggc gcgttcgacg
    24481 cggtcgaggc gatgcgcttc gccctcgagc accaaaatcc cctgacggcc ggcgaagtga
    24541 ccgggggaac gaaatacccg gcggccgcct tctccttgtt gaccccgacg gcgcccgggg
    24601 tttttatttg ggcggtcaag ccggcggaaa acacggcccg ggggggcatc gtcgtccggt
    24661 tgtggaacca gacggccgcg gagacctctt tcagcctcgg tttcaacggc ttcgagattc
    24721 tcaacgcctc ccggacgtcc ctgatcgaaa cgcccctgga aacggcctcg tttctaggca
    24781 gccttctttc cgggacggtg cccggcttcg gttggagggc gtatcacctc gagctgagcg
    24841 gggacgaggt gatcggcaag gggatcgggc ggaaaatcat ccgctgagat caaaaaacga
    24901 ggaaaaaccc ggccgagccg gagaggccgg aaatcgtcct cgatccatcg aagatccagg
    24961 cgtaccggga gtcgagacga aagccccatg tcctcccctt ccccggcagg aggaggaggc
    25021 ccgctcccgc gtgagcctcg ggatcgaacc ggtccttgaa ggcgggggcc ggcacctcga
    25081 tgacgaaacc atagcgggtg atcgaagagg cggctccggc cagggaagcg tccaggccgc
    25141 ccccggccga gagatacggc cggacggccc cggaaaacgg cgccaggaaa acgcccagcg
    25201 tgaccgaaac atggggaccg gccgtgagtt cgacaatgtc gccgtcggag ggatcggtca
    25261 ggacgacggg cgacgtcccg acgaagcggg cgtcgaattc cgcgccgagg gtccggccga
    25321 acatccggat caaggaaaag ccggcgagaa tcccgttgtg ggccggaacg accgggaagt
    25381 cgttttcgcc caagacatac tcctccgcct ttcccgaagg aaaatggtga tggacgcccc
    25441 cgtaaaccgt cagggaattc atccggtccc gcgcttcggc cgttcccccg gccgccagga
    25501 gggcggcggc gaatccccag gcgacggccc ggcttttcat ggccgggatt atatcccatc
    25561 ccgcctcacc ttgaaaggtg aggccgggcc tcctataatc accctggaat tcaccggccc
    25621 ggggtatttc gagccgcggg ctttcccgat atgctttttc ccggccggtc ggcccggcag
    25681 tctattcagc cgacacgggg agaaatttca gccatgtgcg gattggtcgc atttttcaat
    25741 aaggacaagc aagccgccga tccctcgatt ctccgccgga tggccgaacg gatcgcctac
    25801 cgcggaccgg acgaggaggg cgagctcgtc gaaggccccc tcgggctcgc ccacaagcgg
    25861 ctggccgtca tcgatctcca gaccggccgc cagcccatga ccgttgagga cgtcacggtt
    25921 gtcttcaacg ggatcatcta caattacctg gaactccgcg aagagctcgg ccgcctcggc
    25981 cggacgttca cgaccgcctc ggataccgag gttctccttc acgcctacct cgaattcggg
    26041 gccgattgcg tcaagcattt caacggcatg ttcgccttcg tcctttacga ccgccgcctc
    26101 cgccggctga tggccgcccg ggatcatttc ggactcaaac ccctctatgt ccacgaggcc
    26161 ggcggcgcct ggcttttcgc ctcggaaatc aaggccctcc tggcccatcc cggcgtcgcc
    26221 gcggccgtca acgaggaagc gatccgggat tacaccgtct ttcaatacgt tctcggcgga
    26281 gacacgttct tcaaggacat ccggaaaatc cctcccggcc gctatctgct gatcgacacg
    26341 gaatccgggg acgtccggga ggaatccttt tgggatctcg atttcaccgt cgacacctac
    26401 cataccaagg aatatttcct cgaccggctg cgcgggcttc tcgaggactc cgtccgcctt
    26461 cagcttcgga gcgatgtgcc ggtcgggacg accctcagcg gaggcctcga ctccagcctc
    26521 gtgaccctgc tggccgcccg gcagtctccc caacccctcg tctgcttcac cggggcgttc
    26581 cgggaaagct ccgccttcga cgaaacgccc tacgcccggg aagtcgtccg cctggccgga
    26641 gcggaatccc gggaaatcta ccccaagatg gaggatttca tcgacaccct cgccgagctc
    26701 gtctaccaca tggacgagcc ggccggcgga ccgggcctct tcccgcaata catcgtcgcc
    26761 cgggaggccg ccgggcgggt caaggtcgtg ctgaacggac aagggggcga tgagattttc
    26821 ggaggctaca cgcgctatgt cgtcgcctac ctcgaacagg cgctcaaagg cgccatcacc
    26881 gagaccaacg acgagggcga acacatcgtc accctgaagt ccatcctgcc gaaccttccg
    26941 gccctccgca ggtacacgcc catgctccgc cgcttctggc gggacgggtt gttcgacccg
    27001 atggccgccc ggtatttccg cctgatcgac cggaacgccg gcgaccggag catgtacacg
    27061 gccgagttcc gggccggttt cgatgacgac cggatgttcg aacggttttg ctccgttttc
    27121 gatcatcccc ggacgctttc ctattacaac aagatggtcc actacgacct caaggccggc
    27181 ctgccggcca tcctccaggt ggaagaccgg atgaccatgg cctgcgggct cgaatcccgc
    27241 tcgccccttc tcgaccgccg gctggccgag ctcgtcgctt cgatcccgcc gcgcctgaag
    27301 ttcgaggggg gcgatctcaa gcacatcctc aagctcgccg ccggagacgt tctcccgtcg
    27361 cgcatcctgg agcggaagga caagatggga tttcccgttc ccctccacct ctggattcaa
    27421 aacggcctcc gggcctatat ccgcgacatc ctcctgtcca aggcctgccg ggagaggggg
    27481 attttcgatc cggccgccgt cgagcggctc atcgagaatg aggacgctta cggccgcaag
    27541 ctctgggggc ttctgaacat cgagctctgg catctccgct tcatcgaccg gcggggcggg
    27601 acgccgtgat gaacaccgaa aagatcgccg tcgtcggagc cggcgtcgtc ggcattccga
    27661 tggccgccgc cctggcctcc gcgcctcccc cggacgcttc ggcgaaaccg gcccgagtca
    27721 tcctgatcca gcggaactcg tcgacgtcgg gatggaaggt gggcgccatc aacgccggcc
    27781 ggtccccgct gggcggcatc gagcccggcc tcgaacctct catccgccgg gcggcggccg
    27841 ccggaacgct ccaagccacg tccgatttca gcgccctccg ggacgccggc gcgatcctct
    27901 tttgcgtcca gacggacaag cgcggcctcg ctcccgatta cggcccgttg atggaggccg
    27961 tgtcggccac gatccgcgtc ctccgggaaa ctccggccgg ccggcggacg ctcgtcgtct
    28021 tcgagtcgac cctggccccg accacgatgc agacggtcat gcggaagacg ttcaccgacc
    28081 acggactggc cgacggccgc gacgtccttt tagccaacag ccccaaccgg gtcatgccgg
    28141 gttttctcct ggagcggatc cggacgtcgg acaagctcat cggcgggctg cgttcggaaa
    28201 ccgccccggc cgtcgcccgc ctgtactccc ccctcgtctc cggcggccgc cttcatctga
    28261 cgaattgcct gaccgcggaa acggtcaaga cgttcgagaa cgcttaccgc gacgtccgga
    28321 tcgcctgcgc ggctgaactg gtccgcctgt gcgacaagat ggacgtcgat tttcacgccc
    28381 tccgagggga agtcaaccgc cggctcgcct gggaagacgg cgcttcggcc gacgcggcct
    28441 tggttcccgt cggcgggctt ctcgtcccga ccatcggcgt gggcggccat tgccttccga
    28501 aagacggcgt ccttctgctc tggcggatga tcgaggcggg acaagacatg tcggccagcc
    28561 tctttcttca atcgcggcgc atcaacgacg cctcgcccgc ttgggcggcc gatcgtctcg
    28621 aggatctctg gggcccgccg gccggaaaaa aaatcgccct cctcgggacg gcgtaccgcc
    28681 cgaattccga ggacacccgc aattcgccga ccctggcctt ggcggcggag ctcgccggcc
    28741 gcggcgcggc cgtcgtcctg cacgacccct atgtccgacc cgtcgaccag aatctcgaac
    28801 ggaccgggct ggacggatcc ttcacccgcg acctcgatca cgccctgttc ggcgcggacg
    28861 gggccgtgat ttgcgcggcc cacgattttt accgcgagga atggccgcgg atcctgcgat
    28921 cgtttccggg gaagccggcc gtcttcgacg gctgtcacct tcattcccgg gccgagtccc
    28981 cggatgtccc gggactaggc aaggggcggg ccgcccctcc gcccgacttg accggcttcg
    29041 ccttgtcctc tttccgggcg gtcgaaagag gcgtcgccct ggaaatggag gcgttcgccg
    29101 aattcatcaa cgccgacgcc cccgacgccg ggagccgtct cgacctgaat gaggcgggcc
    29161 gcctggccgc cacgtgcgtc acgggctgcc ggctcgccgc tcccgccccc gtcgacgccc
    29221 ttcccgtctt ccaggggtca ttcctctcgc tggccgaaaa aagcctggag ctctcccggc
    29281 gcagggaaaa gaataatccg tgagcgagcg gaaggcggcc gggatttcct tcgacctgga
    29341 taacctctgg tcttatctga aaacccacgg cgatccggga tgggatacat atccctccta
    29401 cctggaccgc ctcctgcccg acgtcctgga tcttttcggc cggcgcggcc tgcgggtttc
    29461 gttcttcatc gtcggccgcg acgccgccct cgaaaagaac cgggcggtcc tgaagctgat
    29521 cgccgaggcc ggccacgagg ccggaaacca ttcgttcgat cacgagccct ggctccatcg
    29581 caaaacgaag ccggagatcg aggcggaaat cggccgggct gaaacagcga tcaccgaggc
    29641 gaccggactc cggccccgcg gtttccgggg gccgggattc agctggagcc cggacttgat
    29701 cgaggtcctg gccgcccggg gctatctcta tgacgcctcc acgtttccga cgttcatcgg
    29761 cccgctcggc cgtctttatt atttcgcccg gtcgagcctg tctgaacgcc agcgggagga
    29821 gcgccggaag ctcttcggcg gcgcgaccga ggggctccgg cccgtccgcc cttatttctg
    29881 gcgcctgcgg ggcgggacaa cgcttttaga gatccccgtc acgaccctgc cgctcctgaa
    29941 aacgccgttc cacttgagct atcttctcta cctcggggcg atttcgccgg ccgcgatgcg
    30001 gacgtacctg cggacggccc tggccgcatg ccggacctcc cggaccgggg tgagctttct
    30061 tctccatccc ctcgattttc tcgcggccgc cgacgccccg ggactcggct ttttcccggc
    30121 catgaagctg gaccggagga agaagcgggc cctggttctt gacgccctcg atattttttc
    30181 cgaatatttt gatattctac cgctgggcgc gtacgccgaa cggtgcttcg acgccccggc
    30241 ggcgctcccg aggaaggccc tccgatgaac gagaccgacc cgcgtccgct ggtttccgtg
    30301 atctgcccgg ctttcaacga ggcggccgtc ctggagcgga acctggaccg gctcgaccgc
    30361 tatctccaaa cgctgaccga ttaccgtttc gaaatcctga tcgtcgacga cggcagccgc
    30421 gacgccaccg gcgatatcgc cgaggctttc gccgccgggc gcggcgacgt ccgcgtcttt
    30481 catcatcaat tcaatttccg tctcggccag gtcctccgga ccgccttcca cggaagccag
    30541 ggggcgatcg tcgtggtcat cgacgccgac ctgagctacg ccccggagca catcggccgc
    30601 ctgatcgaaa agatgaggac gagccgggcg aaaatcgtca tcgcttcgcc ctacgccgag
    30661 ggcggacgga ccgtgggcat tccatttttg cgccggatcc tgagccggac ggccaactgg
    30721 ttcctctgcc ggatggccac ccgcgacttc ttttccgaca agctgaccaa catcaccggg
    30781 atggtccggg cctacgacgg ccccttcatc cggtcgctga gcttgtgggc catggacgtg
    30841 gacatcaatc cggagatcat caacaaggcc aagattctcc gggcccggat cgtggaaata
    30901 ccggccgtgc tcgactggac ggaacagaac gcccgggccg gaggccgccg acggagggtc
    30961 tccaatatcc ggctgatgcg gagcgtcatc cagagcctgg tttccgggtt tcttttccgc
    31021 ccctttctgt tctacatcgt ccccggcttt ctcctcttga tcctttccct ctatccgctg
    31081 ttctggacgc tccggcacac gatccacttt taccgggccc tgtcccccaa tgatctgtcg
    31141 ttcgccttcc ggctgtctga agcgatcggc cgggctttcc aaatggcccc tcactctttc
    31201 atcgtcggcg gcatcgccct gctggtcgcc atccagttga tcagtctcgg cctcctcgcc
    31261 cttcaaaaaa agcgctattt cgccgagctt ttctacctgg ccagcatcac ctaccgcgat
    31321 tgctccaagg gggaaggcga tcgcatccac ctccccagcc ccctgaggaa gaaattttag
    31381 gacgaagcca tgccgcccga gccggcctcc cagcacctga aaacgatcgc cgtcgtgggc
    31441 ggaggcctct ccggaatcgc cgccgccctg gctttggcgc gctcgggccg tttccgggtg
    31501 acgttgttcg aaaaagccgc cggcttgggc ggcctctgcg ccccggtctc catagccgga
    31561 atcgtcgccg accgcttcta ccacgtgatt ttagcatccg accgggccac gatcgcgttt
    31621 ctcgcccatc ttggcttggc cggagagatc gattggcgga cgtcccgggc cggcttttac
    31681 ggccggggac gccttgtccc tttcgcctcg accgctgatt ttctccgttt tcccttcctc
    31741 tcccccggag ccaagctccg cttaggctgg ggaatcctgc ggatcgccct gtccccgccg
    31801 cccggcggag cgaacgagcc ggtcgcccgg gaatggcttg accgccgctt cggccggacg
    31861 gtgacggaaa atttttggga tcctctcctt cggagcaagc tcggcgacgc ccgggaaaag
    31921 acgcccgcct ctttcatgga cgcgaccatc aagcgtctct tcggggcccg ccggggggcc
    31981 ggcggccggg agcggatggg cgccgtccgc ggcggttacg cgccggttct ccgggccgcc
    32041 gaaaaagccc ttcgggattc gggggcccgc ctccggctcg gcgttccggt ccgctcggtc
    32101 gctccggcgg gcggcgccct cgaaatccaa accggggagg gggccgaagc ctttgaccgg
    32161 gtgctgctgg cggttcccct gcccgaagcc ctgcgcctgg ccggagcgcc ggccgaagat
    32221 ccggcctggg cccggttccg ccgcctggag catatgggcc ttgtctgcct cctcctcgcc
    32281 ctccgcaaat cgctctcccc ttattacctc gtcaacctcc tcgaccccga tccgccgttc
    32341 accggcgtca tcgaaagcac gaacgttctc ggccccgaac gcttcggcgg acgtcatctc
    32401 gtctacctgc ccaagtatgt cgcgtccggc gatcccgcgg ccggcctgac ggacgacggc
    32461 gtacgggatc tttttcttcc gtccctgcgg cgcatgtttc cggacctggc cgaagaagac
    32521 atcctcgaag cgcaggttca tcgcgaaccc ctctccctgc ccctcccggc cttcggcccg
    32581 ccgctccgga ccggcggttt tcatagtccc gttccaggct tgtatatcgg cggaacagcc
    32641 ttcatcaaag gggcgacggt caacaacgac gcggcgctta agaccgcgtc cgaagccgtc
    32701 gaaaccattt taggggaata aatcatgggg ttagaaagcg tcttcagcct caaaaagcga
    32761 ggaacgaacg tccgcaccga aatcgccgcc gggatcacga ccttcctgac gatgtgctac
    32821 atcgtcttcg tcaatcccgc cattctagcc gcggccggcg tcccgttcac ggccgcgacc
    32881 acggccacgg cgatcggggc ggccgtcgtc agcatcctga tggggctggt caccaaccgc
    32941 cccctggccc tggcctcggg gatgggcttc aacgccatgc tggccttttc cgtcatcggc
    33001 ttccagcagg cccgtgttcc ctggcaggtg gggatggccg tggtcttggc cgaggggatc
    33061 atcatcctga tcctcgttct ttcggggctc cgcgaagccg tgatgaacgc cattccctta
    33121 aacctaaagc ggggaatcgg ggcaggcatc gggctcttca tcgccgtcct cggactgaac
    33181 cagggcggcc tcatccgacc cgccccgctg accctggtcg aaatcggcga tttcagccag
    33241 aagcacgtct gggtgaccct ggccggactc ctggccatca tctttttctc ggccatgggc
    33301 cgaaaaagcg ccatcctgat cgggctgggg gcggccgcca tggcggcttt gctcttcggc
    33361 ctggtcgagt ggccgtcgtc ggtcatcgct cctctcgatt tctcgacctt tttcgctccc
    33421 ttccatgtcg tcggcggaaa aacggcgctt gttcagatcc tgaccccgac cttgctggcg
    33481 gccgtcttct cgctgatgct gaccgacttc ttcgacacca tgggctcggt cgtcgccatc
    33541 gccgaccagg ccggcttcct ggaaaaggat ggacgggtcc cgggaatcaa gggaatcctg
    33601 gccgtcgatt ccttgggcgc cgcctttggc ggtctcttcg gttcaagttc cattacgacg
    33661 tatatcgagt cggcggccgg ggtggcccaa ggcggccgga gcgggctgat gcccattgtc
    33721 gccggccttt gtttcgtcgc cgcggcgttc ttttcccccg tcatcgggat ggtcggcggc
    33781 ggaatcgcca ttccggccgg ggatcactac gcccaattcg ccaaggccgg ctttactctc
    33841 cccggcgccg atttcctcgt ctatcccatt accgcgggcg ccttgatttt tgtcgggttc
    33901 ttgatgatgc ggatggtccg ggagatcgag tgggaccggt tcgacgaggc cgttccggcg
    33961 ttcctgatcc tgatcggcat ccccctgacc gcgagcatca gtcacggcat cggcttcggg
    34021 ttcatcgtct ttaccctgat caagttcgtt cggggcaaga tccgggacgt ccatccgttg
    34081 atgatcgtcg tggccctggc gttcgcggcg ttttacatct tggaatctat ttagaaatcc
    34141 ggtcgggagc ggggccggag tcctcgatcc tgaagagaat cgccgccggg cattccgcgg
    34201 ccgccgggaa agcggccctg agccgggctt ccaatcccgg cgccgcatcc aagacgtctt
    34261 tttcacagac gaaaaaacgg gctccctttc gggcgtaggc gcggacctca tccggcgaaa
    34321 tcgcctcccg gggaatgttc cagcccttgc ggtccagcca gtagaaaaaa taggacgcgt
    34381 tgtaggcgac cggccggccc gtctcgtccc gggccggtcc gccggtcgcc aggatcaagt
    34441 ctccctccgg aatgacggcg gcaaacggcg cggagcacga gtaaagggaa acgtagtccc
    34501 ggggatggaa ttcgtggacg accaggacgg cctcggcgcc gaagacagcc agcaggccgg
    34561 cggccggaac gatcccccgg aaccggtccc ggcgggacga gagacgatgt ctggccgacc
    34621 gggccgtcac ggccccgacg gtcagggccg ccggggggac ggcgatgacg tgatagtaag
    34681 cggcccaggc gtcgccgacc gtccggagag cggccaggta aaagaggccg accgctccca
    34741 gccaccagag aatgatcccc cgcgcttcct cccgctttgg ggacaaaaga agagccccga
    34801 gaaagagcac cccggccggc gtccagacga aaaggccttc cgtccgggcg gcgccgagga
    34861 gaagcgaaaa cagggttcgc ggcgcccgca aaagatcgag cccgatccaa tgcgtctcgt
    34921 cgctgagtcc gagggaattt ccgaaggaca ggtagagccc gtgggcatgg gcgtaccagg
    34981 ccgcggccgg ggccagggcc aggacgccaa agagccaggt ctccgcccgg aggaaggccc
    35041 tcggcccttt cttccagaga agcaagccgg cgaacaggat cccgatatgg acggccgggg
    35101 ctttgaccag gatcgcggcc gccgtcgcgg cgatcgagat ccaataagac ggccgggcgc
    35161 ctccgtcgag ccagcggaga aaatgaagaa gcccgacgag gaggaaaaga agcatcaatc
    35221 cttcgggttg gagggcgttg gagacgcgga cggccaaggg gctcaaaacg aaaaagagag
    35281 aggccgccgc gtccccggga ggccggagaa ggcgccgggc cagggcgaaa aagaccaaaa
    35341 gcatgagaag cgagacgagc cacgccaagc cgcggccgat ctcctcgtgg aggccgaaaa
    35401 ccttgtaaag cgcggccatg gcccagggaa ggaccgggag ctccatctcg gcgaagcccg
    35461 gaccgtcccc ccgccagtcg atccggggac ggaggatgtt cattccctcc cggtggaaat
    35521 tccgggcgac ggccgcgtaa tcgcattccc gccacgattc ccggacgcgg ccgtcggccg
    35581 gccgaaagac atcgacggcc cggacggccg ctccaagaag aaacagggcc aggaaaagac
    35641 gggcggatga tttcgggccg ggcgggttcc gcggaaacgg accggtggaa ttcacggggc
    35701 cattataccg aaaaagggga acggccgctt cggctgtttt aaagcctctc ccccgtccaa
    35761 ccaattcatt ctcttgacag attcggccgc tccggcctaa actaatccac tcttgattaa
    35821 tcctgcccat cggaacctcg ttcaaaggag ttgaaagatg gtcgaaatcc gttttcacgg
    35881 ccggggcggc caaggcacgg tcgtcgcctc gaaaatcctg gccgacgccc tcgccaagga
    35941 gggcaactac gtccaagcct accccgagtt cggcgtcgag cgccgcggct cgccggtcgt
    36001 cgccttcatc cgcatcgacg gcaagcccat ctacgacaaa agccgaatct acgcgccgga
    36061 ccatgtcatc gtcgtcgatc ccacgctggt cgaggccatc gacgtcaccg acggcctcaa
    36121 ggccggcggc cggatcctga tcaacagcga caagccggcc gagcatttca aattcccgga
    36181 caagttcaag gtcaccacga tcgacgcgac taaaatcgcc gtcgccaaca agctcggctc
    36241 cctggccgcg ccgatcgtca acacggcgat cgtcggggcg gtcatccggg tgttcggctt
    36301 cgcgaagctc gagtccctgc tcggggcgat cgccgaggcg atcgcgatca agcccgaggc
    36361 caaccagaaa gccgccgccg aggcgtacga agcggccggg gcgattgtct gaggaaaacg
    36421 gacatgtcta aaaaaagaga cgccaaaaag atcgtgtacg aatccgcccg ggacttcccc
    36481 caaatgccca tgtcccaagg ctcgatgctc tggaacaaga cgggctcctg gcggaacatc
    36541 cggccggaga tccatcttga aaagtgcacc cagtgcggga tctgctggaa attctgtccc
    36601 gacatgtcga tcgtgatcga gaacgagttt ccggccgtcg actacgatta ttgcaaggga
    36661 tgcgggatct gcgccgcgga atgcccgatg aaatgcatcg tgatggtgga ggaacggaaa
    36721 tgagaaaagt catcatgggc aatcacgccc tgtcctacgg ggcgatgctc tccagaaccc
    36781 aggtcatctc cgcttacccg atcaccccgc agacccaggt cgtcgagctg ctttcggaaa
    36841 tgtgcgccga cggaacgctc caagcgaagt tcatcaaggt cgaatccgag cattcggcca
    36901 tggccgcctg catcggcgcg tccatcgccg gggcccggac gttcaccgcg acatcggccc
    36961 agggcctggc cctgatgcat gaattgctcc attgggcctc gggcggacgg ctgccgatcg
    37021 tcatgggcga catcaaccgg gccatggccc ccggctggtc gatctggacg gatcagaacg
    37081 acagcctcag ccagcgggac accggctgga tgcagttcta cgtctcgagc aaccaggaag
    37141 tcgtcgacac caccatccaa gccttcaaga tcgccgaagc gatcggcatc ccggccatgg
    37201 tcgtcctcga cgccttcgcc ctctcgcata cctacgaagt cgtcgacatt cccgagcagg
    37261 acaaggtcga cgcctacctc cccccctata aaccgtacgc ccggctgacg ccggacgcgc
    37321 cctgcgcctt cgacggcctg accgggccgg aacattactt cgagctccgc tacaagcttc
    37381 aaaaggacat ggaaaaggtc ccggccctgt tcgacgagac gggccgggag tttgagaaga
    37441 tcttcggccg ccggctggag gccgtcgagc cgtacctgtg cgacgacgcc gagatcattc
    37501 tcgtcacctc cggcacggcc ggtttcaccg cccgggtcgc ggtcgatgaa atgcgcaagg
    37561 aagggatcaa gatcggcaac ctcaggatcc ggatgttccg gccgttcccc ttcgacgccg
    37621 tccggcggat cctggccaag gcgtctaaga tcgcggttct ggaccggaac atctcttacg
    37681 gctatcacgg catcttctgc caggaagtca aatcggccct gaacggatcc ggctcctccg
    37741 ccccggtctt cggcttcatc gccgggctgg gcggccgcga catcatggcc gcctccttcc
    37801 gcgagatcgt ggacatcgtc cgccgtcagg acaagcccgc cgaggagatc aactggatcg
    37861 gagtcaaaaa atgagcaaac aaaaaaagat gacgctgact ctcccgacgg aggagctcgt
    37921 cggttcgggc aatctcgcct gccaggggtg cggggccaac ctcgccctcc gatacgtcct
    37981 caaggccttc ggcaagagga cggccatctg cattccggcc tgctgctggg ccgtcatcga
    38041 cgggccgttc ccctattcct gtctgggcat ccccgtttat cactgcgcgt tcgagaccgc
    38101 cgcctcgacg gccaccggcg tcaaggccgg cctggaaatg atcggcgaca ccgagaccca
    38161 ggtcatcgcc tgggccggcg acggcggaac gttcgacatc gggatccagt cgctcagcgc
    38221 cgcggccgag cgcaatgacg acatcctcta cgtctgctac gacaacgagg cctacatgaa
    38281 caccggcatt cagcgctcct cggccactcc gacgggggcc tggacgacga caacgccggt
    38341 caaattcccg aaggaccagc ccaagaagga catcgacgcc attatggccg cccaccggat
    38401 tccttaccag gccacggcct cgatcgccta tccggaggac ctgtacaaga aagccgtcaa
    38461 ggccaagaat atccggggga cgaagttcat ccacgtcctc gccccctgcc cgaccggttg
    38521 gaaaagccgt cccgaggacg ccgtgaagct ggcccggctg gccgtccaga cctcctattt
    38581 cccgctttac gaagtcgagg acggcgagaa atacacgctc aacttcaaga tccagaacaa
    38641 gaaatcgatc gatgagtatc tcaagctcca gggccggttc cgccatctga agccggaagc
    38701 catcgcggcc atccaggccg aagttgaccg caattgggac cgcctcttga aaaaagtgac
    38761 cgcccgctga acggaaagaa gcgatcaaac caggggggcc gttccggccc cccctttttt
    38821 tcgcccggat ttcaagaccg ggataaaccg gcggccgctt tcacggcttt cccgctccgg
    38881 ccggccgggt tctttgggcg ccggcccgga ggcttgattt ttccgccggc cggggataat
    38941 atataatggt tcgattatat aaaggaaatg taatatgaaa accattcttt ccgcggcctt
    39001 gatcgccgga actcttctct tctccgggac cggctgcgca gcccagacgc cctccccagg
    39061 gacgccggcc cccgcttttt ccgcgaccga cctcaacggc gccgtcctgg attcggcggt
    39121 ttacaagggg aaaatcctca tcatcaattt ctgggcgacc tggtgtccgc cgtgccgggc
    39181 ggaaatcccc gatttcgccg ccttctacga ggaaaacaag gacaagggcc tggaaatcat
    39241 cggcttttcc gtcgatgatc ttccgaccgc ccggctccgg gacttcgtcg aaaaattcaa
    39301 aatgacctac cccgtcgccc tcgccgacga gcgcctggtc caggcgttcg acccgggacc
    39361 gtacattccg acgacgttca tcatcgaccg gaaaggattc atccgccaca aggaagtcgg
    39421 aggcatggac aaggccgcct tggccggctg gttcaaacgc ctatcagccg agtgatcaaa
    39481 ggacggccgc ggccccgacg gccggttgca ttcgggagag agagggcatt atactcagga
    39541 atggaatgaa acggcggccc cggaagctct tcctcatcct catcggactg attcccgccg
    39601 ccgtcctccc cttgccgggg caattccatc ccggccaata caccgacgag gcgcccttcc
    39661 ggacctggaa ctcttttccc tatgccggag ccgccgccgc cggccgcggg catacagtct
    39721 tcgcctgggc gacggacgcc tcgtccacgt cggccaaccc ggccctgctc ctcacccttc
    39781 ccaaggcaac cgttgcgctg ggcggctcct tccagtgggc cacggcgatg agatacggcc
    39841 cggtcaattc aggcctgctg atcgcctcct cgcccatcgg gggaagctcg ttcaccggcg
    39901 atcacgccgt cgtttccctc cgggccggcc ggttcgcttt cgccgcgggc gtttttctct
    39961 cggagattta cgaccgcccg ggggccgagg tgtacgacaa cgaaaaccaa gcccgtctct
    40021 ataccatccg cttcacccaa acgggccttc tgcggacctt ccacttggcc gcggccttcc
    40081 agctctcccc ccgcctcggc ctcggggccg gagtgaacgc cgccgcgggc cggttgacga
    40141 tggagttgat ggaggacctc cgctttctgg gcttcaactt caccagcgcg aaatcctcct
    40201 cgctgaaggg actctcgatc aacggcggga ttttctggga catctcgccc tcgcttcggg
    40261 cggccctgac gttccggtcc cccttcaccc tcgaggccgg aaccgaaacg gtcgaccgct
    40321 acgcggcccc ggaggccgga acggagatca tcatccgggg ggaatcagcc gattcgttca
    40381 agcggcccct ggccgtcggg gccggatttt ccatccggca cagcgaccgg ttccggacgg
    40441 cgctcgacgt cgcctggacg cgctggtcct cctacgaagc cgtctggctg ggagatgccc
    40501 ccgtccgcga tttccgggat acggtccgcg tctcggccgg aggagaattc ctctcaacga
    40561 tccggatctt ccggaaaata gttgaggccc ccttccgctt cggcctgatc tacgatcccc
    40621 agccgcccgc ccggccgcgc tcggcgtatt ggggaatcgc ggccggagcc ggactggcga
    40681 tcgacggctt ccgaatcgac ctcggcggct tgatcgggac ggcccaggac gccggcgacc
    40741 gcttgacgtc ggcccggatt tccctttcct gcggatatgc cttttgagac ggccatgaaa
    40801 cgcgcgattc tcctgcttcc ggtcctggcc ggagtcttcg tccttcccgt attcggcgga
    40861 actccggacg ttaaaatcgt cagcgttccg cgatcggcgg ccctggccga ggcggccgtc
    40921 cgcctggacc tggacctcct ggctgaaatc gagggccgcg tcctcatcgc cgcctcgccc
    40981 tcggatctcc ggaaactggc ggaatgcggc cttcagatcg tcgacgaaag cgtcctcttc
    41041 gctccctcgt cgtccccggc gtcttccgtc tattccttct cgcccatcgg cgcataccac
    41101 acctaccggg agctcgccga cgacttagcc gctctccaaa tccagtaccc ggacttggcc
    41161 cgcgtcgtca cgatcgggac cagtcttgaa aaccgcccga tcctcgccct gaaagtgagc
    41221 gacaatcccg gtctcgacga ggacgagccg gccgttctgt ttctcggctg ccatcatgcc
    41281 cgggaatgga tctcggtcga agtcccgttc cttttcggcc gccgtctcct cgaacaatac
    41341 gcctccgatc ccgccgtccg ccgcctcgtc gacgaagcgg aaatctggat cgtgccgatg
    41401 gtcaatcccg acggccatga ctacagcgtc cgggtctacc gctattggcg gaaaaaccgg
    41461 cgggccggcg aaggcggatt gttcggcgtg gacctgaacc ggaattacgg gaaggcctgg
    41521 ggatgcgacg acgagggctc gagccccgac ccggcctcgg acgtctatcg cgggccggcg
    41581 tctttttccg agccggaaac ccgggccgtc cgcgacttga ttctcagccg ccgcttcggc
    41641 gccctgattt ccttccacag ctattcgcaa ctgctcctct atccctgggg atacacgacg
    41701 cttccggccc tccaggaatc cctgctcaaa agcctcggaa cgcggctgac ggacctgatg
    41761 agggcggtca acggacgaga ttatgtctgc gcccaggccg gcgcggccct ctatctcacc
    41821 aatggcgaca cgacggattg ggcgtacgcg gccacgggga tgccggcgta cacgatcgag
    41881 cttccgcccc tggacattct tggcggcgga ttcttcaacc gggaggaaga catcgatccg
    41941 attttccagg aaaacctccc ggccatgctc gccctcgtcg attgggcgat cgcccatccc
    42001 gatcccgagc cggatccggc cgatccgccg cgccggtccg gccggaaaaa gaatccgccc
    42061 ggaccgggaa tcatcagacg atgagcgcct ccctctccga aacctcggcc gtatccttcc
    42121 gggaaggcgt tccctactgg atcttctggt ttttgctcag cctgatcgtc cttctcctct
    42181 ttttcatcct gctgcgggac aaggagctcc ggcgacgctt gagcctgagc ctggccggga
    42241 cgaagcgccg cgttctgcga aaaagccttg agctccgcct tctccgggaa aaacgaaaaa
    42301 aggcctttct ctgccgggag atcggccgga cggtctgggg ggaaaagatc ttcccggatg
    42361 atttccgccc cgcgttcgac cggctggggc ggatcgagaa ggagatcgcc gcccgtcatg
    42421 ccctcctcca ggatctctac gacgagatcc agaaggtgaa aacgggcccc ggggaagacc
    42481 ggaaaaggcg gcggatccgg gagctcgagc ggaaaagcaa ggccgagcgc cgccggctga
    42541 gggaaaaggg aagggaaaag tcgcgggaat acgagcgcct cggttcccgg accgacgaaa
    42601 tgcggatcga gcacgacagc ctcgccgaat ggtacgtccg gatcgacaag gtcaaccggg
    42661 ccatcctggc cgtcctggac cgaatcgaaa aaaccggtta ggaaaggtac aatatccacg
    42721 tcatgtccaa gcccgagctg ctggtccaca tctgctgcgc gcccgacgcc tcctatgccg
    42781 ttcccctgct cgagacggaa cacgcggtga cggggttttt ctacaacccc aatatcgaac
    42841 cgccggaaga atacgaacgg cggctggcgg aaacccggaa gctggaatcg ctgctcggat
    42901 tttccgtcga ggccggccct tatgagccgg agcgctggct ggcgatgacc cgggttttcg
    42961 ccgccgagcc ggaaaagggg cgccgctgcg ccgtctgcta cgccctgcgg ctcgaccgga
    43021 cggcccggcg ggcggcggag cggggatttt ccgccttcgc cacggtcatg agcgtcagcc
    43081 cctggaagga tgcgcgcctg atgaaccgga tcggcctccg cctggcccgg aaacacggcc
    43141 tccgcttcat cgaatcggat ttcaagaaaa aagacggctt ccgccaaagc gtcgagatca
    43201 gccgccggct ggggctttac cgccagaatt actgcggctg ccgatacagc tttcgtcgtc
    43261 cggccgggcc ggcggcgccg gcccgcggag aacaagcccc ttgacgcccc cggccggctc
    43321 cgcctcctcc gccctccgga tcgacgtctt cggcgtcgtc cagggagtcg gcttccggcc
    43381 gttcgtcgtc cggctggccc gccggttcgg actggcgggc tgggtgaaga acgacgcccg
    43441 cggcgtccag atccacgtcg aaggcgggac gcggagcctc cgcgggcgat tcgccgaggc
    43501 cgtggaggcc gaacggccga agctggcccg cgtcgagcgc ctcacccgcc gggccgttcc
    43561 ggcccgggga tacaaggact tcaaagtccg ctcgtccggc cggggcgggg gcttcgtctt
    43621 catctccccg gacatatcca tctgtccggc ctgccgggag gacatccgcg atcccgccaa
    43681 ccggcgcttc gcctatccgt tcacgaactg caccgattgc gggccgcgct acaccatcgt
    43741 tctcgggctg ccatacgacc ggcccaggac gacgatggcc gggtttcccc tctgcccgga
    43801 ctgccggcgc gaataccacg acccgctcga ccgccgctac cacgcccagc cgatcgcctg
    43861 ccccgtctgc ggacccgcgg tggtcctgcg cgatgtccgg aagggaacga tggtccccgg
    43921 cggcatcgac gaagcggccg cccttctccg ccggggaaaa atcctggcca tcaaggggct
    43981 cgggggattc cacctggcct gcgatccgac ctcggccgcg gccgtcgccc gcctccggcg
    44041 ggtcaagaaa cggacgtcca agccgctggc cttgatggtc cgggacctgg ccgcggcccg
    44101 ccggatcgcc cggatcacgg cggccgaggc ggccgccctg gaatccgacc gccggccgat
    44161 cgttctcgtc gggaaaaaaa tcgagctccg cggcctcgcc cccggtttga aggaatacgg
    44221 ggtcatgctc ccttacacgc ccctccacgt gctccttctc gaccggatcg agatgatcgt
    44281 cgcgacaagc tccaacccga aagacgcgcc catcatcaag gacgaatccg aggggttttc
    44341 cgaaatgtgc gattacctcc tgacccatga ccgccccatc cacatgcggg cggacgattc
    44401 ggtcgtccgg gtcgccggga cgtcgccggt gttcctccgg cgggcccggg gatacgtccc
    44461 ggagccgcag cccgcgcccg agggactctc cggccgtccg ccggtcctcg ccttgggggc
    44521 ggagctcaag aacaccatct cggtcttcaa gaacggctac gtcgtcacga gccagttcct
    44581 gggcgacttg gatgaatacc gcaaccggat gtacttcgag gaaaccctcg cccacctgac
    44641 cggcctgttc ggcgtcaagc ccgcctgcgt cgtctccgac cttcaccccg attttcacac
    44701 gacccggatg gccgagcgga ccggccttcc ccatttccgc gtccagcatc accacgccca
    44761 cgtcctggcc gtccttctcg aacacggcct gggaccggga agccgggtcc tgggaatcat
    44821 ctgggacggg tacggatacg gagcggacgg ccgggcctgg ggcggggaat tcctggccgc
    44881 cggctacgag ggattcgagc ggtacgcccg gttcgagccc aaacctctgc cgggcggaga
    44941 cctggccgcc cgcgagccgt ggcgcatggc tctctcttat ctttgggctc ccgggatgaa
    45001 ggcggttccg gcgatcccct cgctgcgggg gatcgaccgg gagcgcctcc gggccgtttg
    45061 gaggatgatc gaggtcggga cgcatgctcc ttcggcatca agctgcggcc ggttgttcga
    45121 cgccgtttcc gctctcgccg gcttggcccc tctccgcaac gaatacgagg ccgaggcggc
    45181 cgtccggctg gaggcggcgg cggcccccgg gcgggcccgg ccgtatgctt tcacgttgac
    45241 ggacggcagc ccgaaaacgc tctccttcgg gccgatgatc gaggaaatcg ccgccggcat
    45301 ccggcgcggc gagtccccct ccgtcatttc cgcccgcttt catgaaactt tggccgcggc
    45361 cgcggtcgaa acagcccggg ccgcccgccg ggacctcgga ctgcggacgg ttgctctcgg
    45421 aggaggggtt ttttgcaacc ggcggcttct cggccgcatc gaggcgcggc tcgccggagc
    45481 gggattcacg gtcctccggc cgcgacgcta ttcgcctaac gacgagtcca tctcggtcgg
    45541 gcaaacggcc tacgctctcg cccggctcaa tcccgtaagt tgaatcccaa ggcttccacg
    45601 gccgatccgc cggttttctc ttcctgggat ccctccggga cgggcggccg acggccggtt
    45661 ttgattttca atccccccgt caccgagaag acgaaattaa ggaccgccgc cgccaggaaa
    45721 gaaacggccc cgagaaagac aaagcctgcg gctccgccca ggagaccgag aaagacggcg
    45781 cccaggagct ttccctgctg ctccgctaat ccggacggta gaaactcgga gaaagagccc
    45841 gtggccccgg cgaaggctcc caggaatatg cccaagacgg ttccaaacag accgccgttg
    45901 agaaaagccg atcccaagcg gatgcttttc gcgatgaata agccctcttc cggatcttcc
    45961 ttcatcagct tgatcgactt gagggacgga accccctcgg gctcctgatt gcagcggggg
    46021 caggtctgga acggcttggt cagcgggccg ccgcacgtcg ggcagatagt cggctcctct
    46081 cccctggttc cgccgcagcg ggcgcagacc tcggtttcga gatcgttttc gaagccgcag
    46141 aagacacaga tcatcttgtc tttttggctc ggcggatcgc ccgcgatcca atccttgccg
    46201 tcgaagtaat accacttgcc ggtttgaaag ccgatggtcc aaaaccggcc tttttcgtcc
    46261 ttgagacgca gctttttgat ctcctcgatg aagctttgct tggagatttc gctgctctgg
    46321 aagcgctttt tcaagctctc aaaagactct tggacgcctt gaaaagtcat ttccattttt
    46381 tcctcgcctc ccgcggcttt tcgatcatgc aaatccttga tttccattgt tttataagtc
    46441 attcataata aataatttag cgtttatgac gaaatcttgt gcaaacggtc gtaactcctt
    46501 ggattctccg ttcttgggtc ccgcagcatc cggttttttt cacacgtttt ccacataatt
    46561 tgtgaaaatt ttcgacccgt ttcaaggcca taacgacctc gccatttgga tcacattata
    46621 atcctgaacg ggagcgggaa tgaaggagag gaggaagacc gctaaaagga cgaacgccag
    46681 gacggtccgg gctgttccca ggggttcatg ctcatcgatg accctgggat gccggagacc
    46741 caggatcaga agaaggaaaa accagatcag ccatccggcc caaacgagga aacccatgac
    46801 gagaagcgcc cccatgacga tgttcgagat cctccggttc cgcgagccga aaacggcgta
    46861 ggcgatatgc cccccgtcga gctgtcccaa cggcagaagg ttgagggccg tgaccaggat
    46921 cccgacccag ccggcgaacg cgaccggatg gaggaccacg tccgttcccg ggccggcgtc
    46981 ccggagaaac aacaagccgg ccaggcggag cagcagcggt tcaccaaaga cgatcgcctc
    47041 ctcgcgggga aaagagggaa cgatccggga catcgccagg ccggcgaaga cggccggcac
    47101 cgctaagacg aaaccggcca gcggcccggc cgcgccgata tcgaacaacc ggcgtttcca
    47161 atggatcggg gaacggatct tgatgaacgc ccccagcgtg ccgatcaagc ttggcgccgg
    47221 aatgaaaaaa gggagggtcg ccgagaggcc gtagcgccgg caggccagat aatggcccag
    47281 ctcgtgggcc aggaggatgg ccatcaggac ggctgcgtag gccgcgctca aaagaatgat
    47341 ccgggggttc cggaaggcgt ccgccggggg ccggaaggcc gggtcggcgg caaaggccgg
    47401 ggcgtaaaga aaattcaggc tccaggacag gccgacgaaa aaagtcgagc cgagcgtcag
    47461 gaggaagagg aggagattga tccagggacg gtcctgggcg aaaaaagaaa agcgcctccg
    47521 ggccggggcc ggagcggacg gcggggacag cggaggaacc ggctcggagg aaagactcat
    47581 gcgcctctta aaaaaaacag gagtgagaac cccactcctg tttcaatcga caatcggccg
    47641 ggaatcagcc gtgaagcttt ttgtacttgg cttcaagggc ctccttcgat tcgacatggt
    47701 cggggtcggg gacgcacgcc gcgaccgggc agacggccgc gcactgcgat tcgggatgac
    47761 tgccgacgca ctcggtgcat ttctcgggat caatgacgta aatcgtgtcg ccggccgcga
    47821 tggcctggtt agggcactca ggctcgcacg cgccgcagtt gatgcattct tcggtgatct
    47881 taaaagccat cgggtttcct cctcggtata atgagatatg aattatagcc cattcgggct
    47941 cgaattcaag gccgccgctc cgggttttcc ctccatccgg acggccttga gggatgcttc
    48001 gtagccgtaa tagctctggg gatattcggt ttggagcttt ttgtacaggg ccacggcttc
    48061 ggcttccttt cccttgagct ccagcgcctc cgcttggcgg aagaggacgg aatccaggag
    48121 ataatccttg ggattctcct cgacgatctt ggccaggatc gccaaggagc cgtcgaggtc
    48181 gcccttggcc agggcgattt cggcctggag gcttttgacc tggtaatact ggaaatcccg
    48241 gccggcgtcc ttgatcgagg aaagagcggc ttccgcctta tcgggacggc cttgttccat
    48301 ccagtatgtc gccaggctga gagaagcgag gcggccggat ttccctttcc cggccagggc
    48361 ttcgagctta gccgcgtttt ccgggacctt ggggagatcg gcccggagag ccagaatttc
    48421 gctgaccgtc cggctccggc tttttgcctg ctcggcccgg atgatccgca ggccggcgaa
    48481 aacgacgccc agggcgagga tcaaaagacc ggccaggacg atctggctgc gccatttccg
    48541 gaaaaaagcc acgactcctt gcatgccgtg gacgagctga tctgtcttca agtgacgccg
    48601 ttcggtccct ttcatggcga tgattccttt cgttggatcg cgatcctcaa agagctcatt
    48661 ttttaccaca tccggccgta aaagaaaagg cggaccctgc ccgtcaggga aacaggcgcc
    48721 gggtcaattc cgccaggcgg gcggcgtaat catccaggct tttcaggtcg cggggaccgg
    48781 gcgcgccgac ggcggccggg ccgtagtgat ctcccttgga atccccttgg atgatcatcc
    48841 cgtgaatcag caaagccttc aggatgtcga tcacggcggt ttccttcccg cccgacggat
    48901 tcccggagga ggcgaaggcg ccgcccacct tgccccgaag cttcccgtga tgcttgacgc
    48961 tttcatccag gagcttttta accggccagg ccatggtccc gtagtacacg ggggatccga
    49021 tgaggatgag atcatacgcc aacaagtcgg agggctcggc ctccccggcc tttttcaaat
    49081 cggccggcag gccggctttc gtcaaggccc cggcgagccg gacggccatc ttttccgtgt
    49141 ttcccgtacg ggaataataa acgaccagcg ctttggccat ggacgcctcc gaagacggta
    49201 tgatagcatg aacgccggcg ccccgctcga ccaggaaaaa aagagggaac atgcgactca
    49261 ccgccgccgt cttgagcctc gtcggcttcg ttcttccttg cgccgggccg ccctacaagg
    49321 atccgcggct ccccgtggag gaaagggtac gggatctcct cgcccggatg acgccccggg
    49381 aaaaattccg ccagctgttc atgattcccg gctcgctcga ttccgggacc gagctcctga
    49441 aagacgggat cttcggcctc caggtcgagg cggcggaatc ggccgtcgaa acggcccgaa
    49501 agatcaatga aatccaacgg tttttccggg accgcacccg gctcgggatc ccgatcattc
    49561 ccttcgaaga agcccttcac gggctggttc atccgggggc cacggctttt ccccaggcga
    49621 tcggcttggc cgcgacgttc gatccggagc tgacgggccg ggtggccgcg gccatcgccc
    49681 gggaagccag gacgcgcggc atccggcagg ttctctcccc cgtcgtgaac atcgccgacg
    49741 acgtccgctg gggacgcgtc gaggaaacct acggcgagga tccctttctc gcggccgaaa
    49801 tgggccgggc cttcgtcgcc gccttcgagc gggagggcgt aatcacaacg ccgaaacacc
    49861 ttctggccaa ttccggagcg ggcggccggg acagttatcc gatccattgg aacgagcgcc
    49921 ttctgcgcga gctctacctc ccgccgtttg aggcttgtct ccggcggggc ggatcgcggt
    49981 cggtcatgac ggcttataat tccctcgacg ggcggccggc ttcggcccag gaccggctgc
    50041 tcaaccggct gctcaagacg gaatggggct ttcagggatt catcgtctcc gacgcggccg
    50101 ccgtcggcgg ggcgacggtt cttcacggca cggcccggga ttattccgac gccgccgccc
    50161 aggccttggc cgccggcctg gacgtgattt ttcaaacccg cctcgaccag gaagatcttt
    50221 ttattccacc gtttctcgac ggaaccatcg accaggccgt gatcgacaaa gccgtcgccc
    50281 gggttctccg ggcgaaattc gaagccggcc tcttcgacga tccgtgcgtc gatccgaaag
    50341 ccgccggcga gtggaacggg catccctcgc accgcgcctt ggccttggaa gccgcccgga
    50401 aatcgatcgt cctgttgaaa aacgaaggcc gcgtcctgcc cctgcggacc gatttcaagt
    50461 cgctcgccgt cctcggaccg gacgcggccg aagcccgact gggaggatac agccggccgg
    50521 ggaacggagt gatctccgtc ctcgacggga tccgggcgaa aatcgggaaa acgaccgaga
    50581 tccgccacgc ccggggctgc ggccgggaga acatcgcgtt cgcggccgtc ccgccggcgg
    50641 tcctctcctg cgagctcgac gggcggaccg aagaagggct ccggggcgaa tatttcgaca
    50701 acaccgctct ccgcggatcc ccggtgctcg tccgggccga tccgcaaatc cagttccagt
    50761 ggacgctcgg ctcgcccgat ccggagaatc tcgaatccgg cgatttctcc gtccgctgga
    50821 gcggccgcct gaccgctccc gaatcggggc gcttccggat cggggtcgac ggagacgacg
    50881 gataccgcct gtttttcgac ggacgcctcc tgatcgacaa ctggatcaag cgcacccggc
    50941 ggacgctcac agccgaagtc gagctcgaac gcggacgggc ctatgacatc cgtctcgaat
    51001 actttgagtc cgagggaggg acggtcttca acctggtttg gaacgtcggg atccccggcg
    51061 gcggcgacct ggaaatggag gaagcggtcc gggcggcggc ctcggccgag gcggccgtca
    51121 tcgtggccgg aatcgaggaa ggcgagttcc gcgaccgggc ccgtttgggc ctccccgggc
    51181 ggcaggaaga gctgatccgg cgagtcgcgg ccgccggccg gccgacgatc gtcgtcctga
    51241 tcggcggaag cgcgatcacg atgagcggct ggctcgacga cgtgcctggg gtcgtcgccg
    51301 cctggtatcc gggcgaagcc gggggcgcgg ccgtggccga cgtgctcttc ggcgaggccg
    51361 acccggccgg gcggctcccg gtgaccttcc cggtgtttga gggacagctg ccgctcgctt
    51421 acaaccacaa gcccaccggc cgcggagacg attacctgga tttgaccggc cggcctcttt
    51481 tccctttcgg cttcgggtcg agctacacgg aattcgccta cgacggcctg gacatcgaca
    51541 aaaacgagat ctcccccggc gggacggccg cggtctcctg ccgggtcacg aataccggct
    51601 cgcgggccgg cgaggaggtc gtccagctct acgtccggga cgttctggcc acggtcgccc
    51661 gtccgatcaa ggaattgaag ggattccggc ggattttcct ccgtccgggc gagacgaaaa
    51721 acgtccgctt cgacgtcggg cccgagacgc tggccatgct cgacgcccgc ctgaaaaaga
    51781 cgatcgaacc cggggatttc cagataatga tcggatcctc cagcgccgac atccggctgc
    51841 gcgggatctt gacggtaaaa taggcgggaa cgcccggcgg cttacagccc gcccgccttg
    51901 aacgtatcgc cctgggtgat gtcgccggtc tggaatccct tggagaacca ccgaacccgc
    51961 tgttcggacg tcccgcgggt gaacgcgacg gggacgacgt atccctgcgt ctgcctcatg
    52021 atccgatcgt cgccgaccgc ggcggccgcg ttcatcgctt cctcgatatc cccttcctcg
    52081 agaaggccgg cggtccggtc ggcgtagtag gcccagacgc cggccaggaa atcagcctgg
    52141 agctcgaggc ggaccatgag acggttgaac tcctcctcct ccagccggtt ccgctgggcc
    52201 atgactttgt ccgtgatccc gagcaggttt tggacatggt ggccgatttc gtgggcgatg
    52261 acgtaggcca gggcgaaatc gcccccggct tcgagacggt cctgcatttg cttgaaaaag
    52321 tcgagatcga tgtagacccg ttcgtccccg ggacaataaa acggtccgga ggcggcgccg
    52381 gcgaagccgc aagccgagga gatccgttcc gaaaaaagaa cgagcttcgg ctcccggtag
    52441 gagcgcccgg cttcctgaaa gagcccgttc cagacgtcct cggtttcagc caggacgacg
    52501 ccgacgaacc ggccttcctg gatttcctcc tcggtgaggg gacgggtttc ccctgccccc
    52561 gtttccatcc ctccctgctc caagatccgg gtcgcgtctt ccggattgcc gccgagaaga
    52621 gcgaacagaa tgaccaggat gatgccgccg atgcccccgc cgacggccac ttgtttgccg
    52681 ccgattctcc gccgatcctc cacgttgccg ctgatcctcc ggtcccgcca cttcatgcgc
    52741 gccttcctta tcccgatttc caggagccga cccggccccg ctctccgcaa ttgataatta
    52801 taccgcaatc cggttcgaaa acgagccgcc gccgcgacga aaaggatcgc cctccgcctt
    52861 ttccgggagg attgacttcc cctccgggcc gtcgtatttt atcctcaaga aacgggaaag
    52921 cccgtagtgt tttttctcct ctgaccggat tgaggcccgg gaaaggatgg ccggagatct
    52981 tgaggcgggg aggctttctt ttccattcca ctcttccgtc gacatcgatc atccgccttc
    53041 attccccttc gctttttttt tcatcgaaaa ggagggaacc atgaaaaaag gagtcgtttg
    53101 gggttttctg ggattggcgt ttttggccgg atcggtttcg gccggcggcc aaggaccggt
    53161 cgccgtctcg cccggagccg aaagcggcga agcggtggtc cggcaagcct gcccgacgtt
    53221 cagctggacg tccgtgccct gggccctgac ctacaaggtc gcggttttcg aggcgcgggg
    53281 gggaggaatc gttcggacat atgaagccat ggccgcccag gccgcgcccg tcctcgtcaa
    53341 ggagatcccc gggcaggcgt tctcctggac gccgtcggcc gatgaagccc tcctcaacgg
    53401 gggacgctat gtctggtacg tcggggcgat ggtcaatgcc gcccagggga gttggtcgga
    53461 aggccgggcg ttcgtcgtcg ccgaggggcc gggctggggc gtggtctcgg agccgccgac
    53521 gggcggggag cgggtgaaag ccgcggaatc cgccctggga agcggaaagg cggatttgac
    53581 agggactgcg aaaggagaat tcaacctggc ggcggatttc cggccgcagg attggatcgg
    53641 cgtcgaaggg acggaaggag cctccaacac gctctacggc ctggaagccg gctttgggat
    53701 cacgaccggg gtgaataatt cctttttcgg ccggcaggcc gggtacgccg acacatccgg
    53761 gtacgccaat acgtccgtcg ggcgggccgc gggaaaattg aataaaaccg gatgggaaaa
    53821 cacgtacatc ggattccagg ccggcaataa aagcacggct gacgaaaacg ttttcgtcgg
    53881 agcccgggcc ggcttggaga atctctccgg atcggacaat tgcttcgtgg ggcatgaagc
    53941 cgggttcaag aacgtgtccg ggcaggggaa ctcgtttttc ggctttcaaa gcggctacgg
    54001 caatgacgga agctggaaca cggtcgtcgg agcctatgcc gggtatgcca tcacccgggg
    54061 aatggacaac gtcttcatcg gcgggagctc gggggaacaa aacagcggca acgacaacgt
    54121 ctttatcggg aaagaaacgg gccgcttgaa caacagcgga tacggaaatg tcttcatcgg
    54181 caattgcgcc ggatacaacg aattgggagc caacaagctg tacattgaaa acaccccgtc
    54241 aaccactccc ctgatctacg gcgacttttc cagcgacaag ctgagattca acggcgccgt
    54301 cgggatcaaa aaaacgccct cctatttgct ggaagtcggg acaaacggag ccttttgcaa
    54361 cggcggagtc tgggtcgacg gctccagccg ggagttcaag gaaaacatcg aaaccctgcc
    54421 ggccggagaa gccgaggccg ctttcgacaa gctcgaaccg gtcaaattcc aataccggga
    54481 aagcgacggt gaaacctaca tcggattcat cgccgaggac gtccccgagc tggtcgcgat
    54541 gaacgaccga aagggtctga acgccatgga cctggtcgcc gtcctgacca aggtcgtcca
    54601 agagcagcgg tcggcccttc gggaacaaca ccgggaaatc ctcgccctgc gggaggatat
    54661 cgcgaagctc aaggaggaca tccgccggga aaaatgaaat gcgcccgctt agtttctaag
    54721 gaggaagatt tttacaaaat gaaagttaaa atcaagtaat acgttgatat atttataaat
    54781 atggccgttt tttcacttgt catcggcgga aatatccatt aacatgcttt cagtagggcg
    54841 ggaagttgac agggaacgca tcatgagccg aaccgttgtc cctgactgtc aagttatttt
    54901 caaaattatt gtttcgaatg ttaaagggtt gatatgggtt aaatcaagcc gatgtcgcac
    54961 gacttgctgg gagacgggtt ttgcatgttt caaaaactaa aagcgttttt tttctttgta
    55021 aaatgtcttg ggtaaagcat aagtattaaa ttgcagggaa ttccgggctt actcggctcg
    55081 gccttaataa ctctatggag gaggtttcga tgattccaaa acgtgtagac gagcgattga
    55141 ttaaaagtgt ccccagattc caacaggttt taaaaacggc gaaagacaga gacgtcaacg
    55201 aaaccgatac cctctccatt attaaagata tctttgcaga aatattcggt tagctagtgt
    55261 atagggatgg ttgcaatttg gattaggagg cgggccatcg taaactctct tgatcgccaa
    55321 atctcgagag gaggattttt actacgatgg cccaaaccca ttataaggca ctctttcaga
    55381 aggcgctatt acagttcatc accgagccgg atcccttttt agctatgctc cagtgggtca
    55441 tgaccgaaat gatgcgaatc gaggcggaag cgaaagttgg cgcggtcaaa gggaagcaca
    55501 cgaatgagcg aacaacgtat ttttccggag ctcgagt
//
LOCUS       DAOOXH010000008        47288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_626_length_47288_cov_8.043211, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000008 DAOOXH010000000
VERSION     DAOOXH010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 47288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47288
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_626_length_47288_cov_8.043211"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PKV64_02135"
     CDS             <1..290
                     /locus_tag="PKV64_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15911.1"
                     /translation="AAAGTSGKDGGEIRASPAAGYRQIFLLLGLLYPPEDVGRALQNY
                     AEGTKWAVDYALELLEAVLPKKMKDAVLPVLEDAPADVKARSARRILKTLG"
     gene            301..1977
                     /locus_tag="PKV64_02140"
     CDS             301..1977
                     /locus_tag="PKV64_02140"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPN15912.1"
                     /translation="MATLYVYPKTAPPFSALLEKEETVLGRALDADVRLPDAYISSRH
                     ARIRRTSRGWTVEDAGSKNGTMVNGRRIVEPVLLAPGDEISVGAVRICFDRPWTAAVE
                     ITDSPNPAVRVSAAVPSRDIITRSTEAGVRRMEPSGPGTRADQLLLVINEVSRALLFH
                     KPLQELLEYVMDLIASHLPMDRGALFLKEGDPEKLVPRAVRIGNPQLKSGPMQISRGL
                     VEMASSRRLSVLTTDAAEDPRFADRQSVIDMGLHSAMCVPLETEKEILGVVCAERVSS
                     RESFTDEDLRLLTLMANLAAVKIENARLVEDGLIRDRMERELRLAAEIQRGLLPKECP
                     VCGDFEIIAESTPSLHVGGDYFDFIPLADRRLGTTVADVAGKGVSASLLMASLRAALQ
                     SEARPGMGPADLAFKLNNFIHRSSAPNVFITFFYGELDPSTGDFRYVNAGHNPPLLLK
                     AGGKELSLEGTGMALGMLPESTYEEGRADLEAGDILVLYTDGISECRSPAGEEFGTGR
                     IVRAVRRLAGRPAGEILGAIFSDLREFTGGADPDDDRTVLIIKRPAERAS"
     gene            2202..3386
                     /locus_tag="PKV64_02145"
     CDS             2202..3386
                     /locus_tag="PKV64_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15913.1"
                     /translation="MKTRIVLIAAAGAALLGVFSLAGADVESSRRNDGPPVSAAPAET
                     VLRADPVFDDLASFLAGFPCVSSPYKEFQETAEYREFADALEKNWAELEMKRLGPVRH
                     WARSEIGEAAAATTTLFYPFGGPDALTAVILFPQAARHLLLGLEFVGRLPVFTADQGG
                     AAARYIQDLRASLDDFFKKSYFITRNMNDELAGDKVDGVLPLLCFFLKRSGNVIASIT
                     RLDITDQGGILEGPFPGEPKKRRRPFGVRLAYFAEGADVLKELSYISCDLSNGAFREN
                     SPLAFYLGTVAFETTFLKSASYLMHYRDFSRIRVLILERSRFVLQDDTGIPFRYFKPG
                     RWTARLYGEYSDPVKDFANVGQPDLKKAFADPARVKSLPFRLGYHWGSDKGLLLYFMR
                     KG"
     gene            3383..4714
                     /locus_tag="PKV64_02150"
     CDS             3383..4714
                     /locus_tag="PKV64_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPN15914.1"
                     /translation="MKTKFSLTTATALVVANMVGSGVFTSLGFQAAGISSGPALLLLW
                     ILGGAIALFGALTYSEAGVLFPRSGGEYHFLTEMFHPALGFLAGWISFLVGFAAPVAA
                     AAMGLGRYLTSALAVPQKTGAGLSSSSLIAVGVVAVLTVLHSLDKSLGARFQNVITIF
                     KILFIVLIIVLGLAMGEPAGLSFAPTSRALRDVLSPAFAVSMFFVTFSYSGWNAAAYV
                     AGEIENPRRNLPRSLIAGTLIVSVLYILLNFVFLRTVPIAELSGKLEVGYVFAGRVFG
                     AAGGRLMGAVIAFLLLSSVSAMIIAGPRISRVIGEDYRLFRFLGKTTAKDIPAAAVVV
                     QGLLSVFYIVTSTFDQVIVFIGFTLNLFTFMTVLGVMLMRRKRPDLPRPYRTWGYPWT
                     PAAFLVIHVYILAYGLIYRPRESLAGLGLTAAGLVVYAIDRKTRRVKPDSV"
     gene            complement(4818..6050)
                     /locus_tag="PKV64_02155"
     CDS             complement(4818..6050)
                     /locus_tag="PKV64_02155"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPN15915.1"
                     /translation="MPAKRSSFDRREFLKKSLLGVAGAGAWGAVPGVLKAGVQASSAP
                     AAAKPGVITRPLGKTGVALPVVSMGVMNSNNENLIQAALDGGIVHLDTAHGYQRGTNE
                     GVIGKVLKGRPRESYFIATKVPGEPQDRRTGNFLAETTAGPFLEKFELSLRRLGVEYV
                     DILYLHNVLTKEAVLFEPLMNALVKIKQSGKARFIGVSTHGNEHEVIRAAIEGKIHDV
                     VLTSYNFRQTNRVALDAAVADAAAGGIGIIAMKTQAGAYWDRGKTRPINMKAALKWAL
                     GNPHITTAIPGMTTFDQLALNLQAMTDIVLTDAEKADLELGGGGEPGLYCQGCQTCLS
                     QCARRLPLPSLMRGYMYAYGYRNLGEAYDLVASLGVDRNPCADCASCPVQCALGIDVR
                     GRATDIARIQDAPADLFG"
     gene            6285..7220
                     /locus_tag="PKV64_02160"
     CDS             6285..7220
                     /locus_tag="PKV64_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HPN15916.1"
                     /translation="MTIDQNPPSIGRRWVTAARPWAFPASTMPVIFGTSLAVVAGGAS
                     LNILLFGGAFAAMVILHAAANMLSDVFDFRHGVDTQATPVSGAIVRGWLTERQVARGA
                     VALFAAGIALGLVLAWRTSWILLAIGAAGVLIGAAYTLLKARALGDAAVFLNFGVLGA
                     LGAWVVQTGRFSWLPVAWSVPIAMLVSGILHANNWRDIATDSGRRIRTCAGILGDRGS
                     IVYYGFLLFGSQLLVIAFAGFPLVLPGLPRLPCPCLITLAVLPEAATLWRRARRRHRP
                     KNPLDFVVLDGATARYNLHFGLLYTAGLWLGRWLG"
     gene            7396..7929
                     /locus_tag="PKV64_02165"
     CDS             7396..7929
                     /locus_tag="PKV64_02165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPN15917.1"
                     /translation="MAGSLVLLVVLGAALDRDFASLLWADVRNESGRKILRGVVSAVF
                     LYAVFWAGNAVSRVLFSFAEGGISEVYGFKDGASTFRIGFLLGLIIGPGEELFWRGFL
                     QRRWAAWFGPGRGFLAATVLYAAVHAGSGNPMLISAAAVCGVFWGFLYLRTGSALLCA
                     VSHTLWDLAVFLVFPFA"
     gene            complement(8119..9087)
                     /locus_tag="PKV64_02170"
     CDS             complement(8119..9087)
                     /locus_tag="PKV64_02170"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPN15918.1"
                     /translation="MPLDRTEALRAEYRARINRVLDYIDLNIRRDLRLDELARTAHFS
                     RFHFHRIFSALTGETLNAYIQRRRAELAAAALLLHPKATITEIALDYGYSGSDVFARA
                     FRERFGMSASEWRAGGGEAWRKKRQTNGKNRQAEGKISQEIFGALDDDEDALISFRRS
                     FMDKSKFKVEVREAPEMTVAYVRHVGPFQGMSQAFEKLMRWAGPRGLLRFPETKTLGI
                     YHDDPDITANDKLRSDACITVPPGTNVGGEIGAMTVPGGLFAVARAEVSPDEFGEAWN
                     ALMRDWLPGSGYEPDDRMCYELYLNDPKTHPEGKFIIEIHEPVRPL"
     gene            9290..11224
                     /locus_tag="PKV64_02175"
     CDS             9290..11224
                     /locus_tag="PKV64_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPN15919.1"
                     /translation="MKRLFFILGILLAIGPAVFSQAPDDIVSVEVVAPAGGFTAGRTV
                     QAALKLTIRPPFHINSDRPADEDLIGTTIVFRSQAGVTYGWPAFPRPVRRKLSFSETP
                     LSVFEGVITVPFDVTVAADFAGKEWTIAGTVSYQACDDQTCLPPRDVPFSDTVGVAFP
                     PAAAPSPSPPADKQAASTAEAPVRAEEPPAAEAADSVSPEAKPESGPPALEPAAGPSG
                     REVPFEGKGLPVIFALVFLGGLGLTLTPCVYPIIPITISYFGGQSEGKKGGVFLHAIL
                     YVLGMAVTYSILGVIAAFTGGLFGAALTYPPVLIGIALIMIALSLSMFGVYEFRMPAF
                     LNTLAGGSQKGYFGTVLMGLTVGIVAAPCIGPFVLGLLTYVGNRGDVLLGFSLFFVLA
                     LGLGVPFLILGVFSGALGRLPRSGAWMIWVRKIFGFILIAMAAYFLKTLFPNTLFYHF
                     VLALIMLVAGLYLGWIEPTKTGGKAFSYVRALTGVVFLVLALLFAVRGVEEYVGGTAS
                     AGAAAGLVSEAIAWRPYSEEALAAASAAGKPVFIDGFADWCIPCKELDRMTFSRPDVV
                     AASRSFVMLKADLTAGGDEKVKAFSQKYGIRGVPTLLFLRPDGTEIEELRGTGFEPAE
                     AFLARMRKALERSGEKAAGR"
     gene            11221..11553
                     /locus_tag="PKV64_02180"
     CDS             11221..11553
                     /locus_tag="PKV64_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HPN15920.1"
                     /translation="MKIEGVPFTVTDWGSVPAVEHKGASGTSFWRTVEAGNLRVRMVA
                     YSPGFASDHWCARGHALTVLDGEITVALKDGREFVLTAGMSFQAEDDEACPHLARSGF
                     GARVLIVD"
     gene            complement(11577..>13363)
                     /locus_tag="PKV64_02185"
     CDS             complement(11577..>13363)
                     /locus_tag="PKV64_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN15921.1"
                     /translation="VTDLQKETLDSIRRNGYRLLKLINNLLDLNKLEAGRMRLRPKTL
                     EFNDYVETLLASVKVLADQKNIRLFFQHPLTEVELTIDPDQFEKVVLNLLSNALKFTP
                     ENGRVTLYVEERPETVLLTVEDTGSGLPSHMLEAVFDRYRQVEGSAARAGTGIGLALA
                     KEVVSLHGGLIRVESEPGEGSRFIVELLKGTRHFSEEVMDRRQSDQPVAFRKRATDIE
                     PEGVRVQDIISNVRDLQLIDLEKVEFPITPPAERRHEYQLLVIDDSPEVLKLMHLLLK
                     DEFDLDFALTGEDGLRILHERGPDLVLSDVMMPGMDGHELCRRIKSDEAVRHTPVLLV
                     TARSASEMLAAGMESGADDYIVKPFNAVELKARIRSHLRMRQVEAELALANRNLKMRT
                     SDLVERQRMLFISMVKSLVSALEAKDFYTRHHSARVTEFSLKIARHMGFGERELDDLE
                     LAGLLHDVGKIGIPENILNKPGPLTGEEYKAIKDHPVFGESILKPVVELTQIAKIVRC
                     HHERYDGKGYPDGLKGLEIPLGARIMTVADSYDAITSKRPYRGEESHHFAVKEIIRCS
                     GSQFDPEVIQHFIEVAKTFSGLGPGGTP"
     assembly_gap    13364..13463
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13464..13918)
                     /locus_tag="PKV64_02190"
     CDS             complement(<13464..13918)
                     /locus_tag="PKV64_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15922.1"
                     /translation="MRNKDKSLFERLGEEYDLRGRTQWVIFFSAILFAAFILLDRLYV
                     PPMFHTFLLIRLAVLAGHGLLLLILRRTRSNRGAEMVAIALTVLDAGGISIMIQMLGG
                     VSTAYVQGLYLVIMGVVVLVPLRFAASASLYALIWLGTAVPSIFRPPASG"
     gene            complement(13920..17459)
                     /locus_tag="PKV64_02195"
     CDS             complement(13920..17459)
                     /locus_tag="PKV64_02195"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPN15923.1"
                     /translation="MPEIRPKDSTTSPRLRVLAVDGRPGRLHSFKKALNGNADLIAAI
                     GPLEAFYWAEKLQTVDCLILRDPASISAEPGAFLRSLLQSFQSTERMVKIVVAGPEEA
                     AALRRSLLISPSDLVLESPVETEALCLEVRKRLSRLAVEKRAVVRIPISEKNPIRVEI
                     EGGGGPAVVRDLSETGMFLQTAAGLGVGARRPFVLHVADGECWKVEGIVVRSGEGEGG
                     VGIAFRPADEEARRKIFSRLAETVSPKDLAELKLRYPELHTSAMVAFSSPDKIRGLLA
                     GARRARTEITALPAHVRQPATLTLEHVDPGRICVLSGKSLNLHFKTGDPVFMSFQSGY
                     ATYNFETTVRRLGENGDFLECFYPRILFYSEKRSLKRESPQDGLRLELVLPPPFSAGI
                     SGPVVDLSDTGASFIADAGGLALLPGTPVGTVRIFDNGRLIREERGEIRHAVRTEEDG
                     SPAFRYGLQFGIGRLSIQAVHPHRRSTDVPPAAAAEPGSGADPGLPDILRELSHRPPA
                     VIRLENERGEEIVGLLNTSYPPDGNPVPVVIIPPAFGKTKETLFGLALTLVENFRRAG
                     RRLAVFRYDGIRCKGESHKDPEAAEPPFEMVDSSLSQGAADLKTVLEWLEMNPTIKAG
                     PVILATFSLSALEARIALRDPAVRRRVHYWIACMGTLEFRDLMNRVNCGLDLLEQHQL
                     GIDLGVIPILGNLVKMRHYAADVVASGVATLDQAREDMRHLNLPVTWIYGKHDNWVKA
                     EFVRDVMSIKAEASREVFSVPLGHNARNSEEALRLFGTVTSLVHRFLHGTMIEAIPPE
                     RKNLEYLRRAEKDRLPGRILKNKHTYWTHYLVGEKGLLGFDTMALSDDYVRLMEDQRK
                     ALAFDPEDRFLDLGGGTGNFIAHILQSGGPLPSRLVLADLVPEALARAFDKLTSLEPS
                     LKNAGRLSVLGLDVELNRLIPIRRFLAGEIGRFEDLAEQIENLPLQSALRIDAAYSPR
                     LHRILRGEEITPETERFLKSTFELAEGRVIRDFNLAARWTAGLAPGHPAFRKLAFPGG
                     RETAFFLPFKEERFDKILMSLVLSYIFNPVETLREVRRLIAPGGRLVLSSMRPDTDAS
                     GLFTRLLEKIEAAPEDALPIPGPKSRILESMRSFLNDAQALVDLEEAGTFDFFDPGKL
                     ELLLGEAGWTQVEILPSFGNPPQGYVVVAKPRN"
     gene            complement(17707..19833)
                     /locus_tag="PKV64_02200"
     CDS             complement(17707..19833)
                     /locus_tag="PKV64_02200"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPN15924.1"
                     /translation="MKKILLFVLILATVIVAAVLARPPQTAAAEVNPFLVEWTTPFET
                     PPFDLIKEAHFLPAFREAMARQKNEVAALTSSIEPPTFANTVEALEHSGALLKKVAYV
                     FFALTNNHTNPELQKIEAELAPLLSKHSDDISLDPKLFERVKAVYEKKEGFGLSPEQA
                     RLLDLTYKHFVRSGAALDEAKKAELRKVNEELAVLNQKFGQNVLAEDNGFTLVLDKKE
                     DLAGLPEGVIAGAAEAAKEQGQEGRWVFSLHKPSCIPFFQYSSRRDLREKLFKAFISR
                     GANGNAADNRAIASKIAALRVIQANLLGYPTHADYVLEVNMAKTPAGVYKLLDQVWTP
                     ALEMAEKEARELEALMNKDGVVGKLEPWDWWYYADQLKKAKYDLDDEALRPYFELKAV
                     LRGAFDTAGKLWGLTFTPRTDIPVYHPDVEVFEVKEADGRHVGILYSDYFPRPSKRGG
                     AWSSAIRMQSVQDGRRVTPVVYNVGNFTKPTADQPSLITFEEALTLFHEFGHALQDLL
                     SDVTYESIGGTNVPRDFVELCSQIMENWAADPEVIKTYAKHYRTGEPIPLDLLDKIEK
                     TGQFNQGFTTVEFESACYLDMAWHTLAEAAPQDAVAFEDALLKKIGMIPEIIVRYRTP
                     YFAHIFSGGYSAGYYSYLWSEVLDKDAFQAFKETSLFDPKTAKSFRENILSKGNTREA
                     MDSYKAFRGREPKVEALLKARGLLKS"
     gene            complement(19875..20507)
                     /locus_tag="PKV64_02205"
     CDS             complement(19875..20507)
                     /locus_tag="PKV64_02205"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPN15925.1"
                     /translation="MNEALKSLKDRLRRPLPGPPAQLRMMPLPPPPGQKTYVEAGGDC
                     LPGAVLALLYPRNGKTHLVFIRRPQNVVHHKGQIAFPGGHVETGETPAEAAVREAMEE
                     IGVVPSTLRLLGALTPLYIPPSGYCIYPFVAETDVPPAFVPQAGEVDEIIEAPLADLL
                     DDHNLRRETWTFETGPRLVPFFAVGPHKIWGATAMVLSEFLEILRPPDGR"
     gene            20655..21617
                     /locus_tag="PKV64_02210"
     CDS             20655..21617
                     /locus_tag="PKV64_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15926.1"
                     /translation="MSFLPLTEKARRLGACLALVLFSAAAGSVSAQSLDIPSKKWGLS
                     FGNSKEFTGLRFNFRDRGVIRITGVNVTLWTPRTLGEEDDSTVTGLSLGLVPGAGRMR
                     GVQLGLLGVAGNRSIAGVSAGLLGVGAGEDISGFNFGGLGVGAGGSVAGINLGGLGVG
                     AGENVLGINIGGLGVGAGKNVTGINIGGLGVGAGGRLAGINIGGLGVGAGERLAGISI
                     SGIGAGAESVAGLAIAGIGVGGRRLSGVFAAGAVIKLVDDGRLRGVAVSPFNQLKGTL
                     TGLSIGIVNYARRIEKGIQLGLVNIVRENKPGLRVLPLFNTDFR"
     gene            complement(21668..24340)
                     /locus_tag="PKV64_02215"
     CDS             complement(21668..24340)
                     /locus_tag="PKV64_02215"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPN15927.1"
                     /translation="MMNKDFKRIFVLAALWPVVFAAAKQRPVPAPEPGQKIVQAFRLE
                     EPVKIDGRLDDSAWKGEGANGFTQNDPQDGQPSTETTEVWVAYDDEALYVAAKCRDSE
                     PDKITGLLGRRDSMVESDWFYFAVDPYYDRRTGYMFCVNPSGSIMDFALNNDIADDSS
                     WDGVWESKAAIDGDGWNVEIRIPFHQIRFSRMEEYVWGVNFRRKIRRKNETASFAWCP
                     KNETAFVSKFARLEGIRGISPGVHVEITPYATGQAQFRPAEEGNPFETGRKASGNAGF
                     DLKAGLKSNLTLDATVNPDFGQVEVDPASVNLTAYETYYEEKRPFFIEGASIFNDFGR
                     GGIYFNANINWPNPKLFYSRRIGREPQGSPSGEGYVSLPDRSTILGAAKLTGKIGGWN
                     VGFINALTSREYADIDLSGLRSREEVEPLSYYGIFRIQKDIDSGRHGIGLLATGVVRD
                     LDNPALEGLLNRNAFSLAVDGWSFLDAKKDWVFGGWIGGTRIGGSSENILRIQNSSIH
                     YYQRPDAAHVEVDPAAVSLSGWGTRVHLAKQNGRFIWLSSLGMLSPGFNPNDAGYQAG
                     TSDVINVSVVPGLNFTEPGKIFQQILVGAGGFANWDFGGHNVGKGGILIAEGLFKNFW
                     YFSAQLFAGAGSLNNRQTRGGPLTWTPGMGNVFLMLSTDGRKKVVLEGSGGITDGGRD
                     GRSWNAGLSLRWKPAANVSFSAGPSYLAETNETQWVDRFADASMTPTFGNRYVFARLE
                     QRVVSAEIRLNWTFTPRLSLQAYLQPFIAVGAYSRFKELARPAKYEYNLYGENSSTVE
                     EAGGGYTIDPDGTGPASPFTFGNPDFNVKSLRGTVVFRWEYLPGSLIYLVWTQNRADY
                     ANSGILNLRRDFSDLLTAPGENIFMVKVSYRWNM"
     gene            24534..25385
                     /locus_tag="PKV64_02220"
     CDS             24534..25385
                     /locus_tag="PKV64_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HPN15928.1"
                     /translation="MKKALLLLTAALVCAGLLPGVSADSPPSTREMNLLCWQEFGELV
                     PARIRTVLLPTGSLEPHGVVPNGTDSLAPEAMARDIAGRVDALIAPTLPYGMTADMKA
                     YPGAVSISKEAYAAFAREILENLADNGFQNVIILNGHGGNTAVLNEVSSLVSNARRVR
                     ILLVNWWTLADDITKDVFKQDGGHAGNNETAYIQAIVPNHIHPERYRPEMAAPLPAPA
                     GAFAAHPVTTSILLYQKGQGYPTFDPAQAKDYFRRVNDRVAELILDAVKKWDAAGVYG
                     RDRDGGR"
     gene            25382..>25506
                     /locus_tag="PKV64_02225"
     CDS             25382..>25506
                     /locus_tag="PKV64_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HPN15929.1"
                     /translation="MSLRFYQVDAFTSEIFQGNPAGVCPLESWLPDGLMQNIAME"
     assembly_gap    25507..25606
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <25607..25942
                     /locus_tag="PKV64_02230"
     CDS             <25607..25942
                     /locus_tag="PKV64_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015329166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HPN15930.1"
                     /translation="FGTQAEIEAMRPDFGKLGRVEARGVIVTARGTEVDFISRFFGPQ
                     VGIDEDPVTGSAHTSLAPYWAEKLGKTEMEAVQLSRRGGRLKVRLRGDRVDISGRARA
                     YLEGRIEME"
     gene            26029..26772
                     /locus_tag="PKV64_02235"
     CDS             26029..26772
                     /locus_tag="PKV64_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020888211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidinotransferase"
                     /protein_id="HPN15931.1"
                     /translation="MVRTPGPDAAAGLTTAGLGKPDLALLLAQHAAYIETLRGLGLEV
                     EVLPASPGFPDAYFVEDAAVVLPEIAVLTRPGAPSRRGETASVESALARHRRLARIDL
                     PGTLEGGDVLWAGGRFFIGLSVRTNEDGALQLGRILESCGYSWTAVPVEKGLHLKSFV
                     NTLGGRTLVVASRFEGREFFRSFDRIVLDPGDDYAANTLWVNGTLLVPRGFPRALEKY
                     AGLGLPLIEVDTSEMRKMDGGLTCLSLRF"
     gene            26777..27952
                     /locus_tag="PKV64_02240"
     CDS             26777..27952
                     /locus_tag="PKV64_02240"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPN15932.1"
                     /translation="MKTPEARSGRRRAGTVLAGMFLALAPPALAAPPLFASSEGRIPL
                     IDLAGEAGRRTIVDRESGQYLGHPTTVLLEDGRTLLAVYPKGHGRGPIVVKRSRDGGA
                     TWNGRLEVPDNWATSLETPTIHRVVEASGKKRLIVWSGLYPARLAVSEDDGETWSPLR
                     PAGDWGGIVVMASVVSLNRPGGYLGLFHDDGRFFRRDSGPSDPAVFTIYKTFSNDGGL
                     TWTEPEAVWSSPALHLCEPGALRSPDGRELAVLLRENSRRKNSHVMFSRDEGASWSTP
                     VELPGSLTGDRHCPKYAPDGRLFVSFRDMAPDSPTRGDWVAWIGAYDDLRGGREGQYR
                     VRLMKNHKDADCAYSGVEVLPDGTIVATAYGHWTENEEPYIVSVRLRLAELDARLGR"
     gene            complement(27980..30202)
                     /locus_tag="PKV64_02245"
     CDS             complement(27980..30202)
                     /locus_tag="PKV64_02245"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HPN15933.1"
                     /translation="MAKQTIIWTETDEAPALATYSLLPIIQGFTKGSGVEVETRDISL
                     AGRIIANFPDDLRDNQRIQDHLAQLGELTARPEANIIKLPNISASIPQLQGAIRELQS
                     KGYALPDYPERPQNEAERVIQARYAKVLGSAVNPVLREGNSDRRAPLSVKNFSKTHPH
                     KLGPWKPDSKACVVHMTAGDFYGHEKSAVNQKERPIRIELTGKDGTVKVLKEDLTALP
                     DEILSATFMSWKALRKFYAEKIEEAKAQGLLLSLHLKATMMKVSDPIMFGHAVSVFYQ
                     KALDKHAAVLKELGVNVNNGLGDVYAKIQVLPEAKRAEIEADLKAVYAERPALAMVDS
                     DRGITNLHVPNDIIVDASMPVVVRESGRMWGPDGTLHETMAMIPDRCYSTIYKEIIEN
                     CKKFGAFDPATMGSVPNVGLMAQKAEEYGSHPTTFEIPFDGTVRVVTPCGRTLLEHEV
                     EAGDIWRMSRAKDIPIRDWVKLAVKRARATGAPAVFWLDRNRAHDAQVIAKVERYLKD
                     HDTTGLDIRILPPVEAMRHSLDRIRKGLDTISVTGNVLRDYLTDLFPILEIGTSAKML
                     SIVPLLGGGGLFETGAGGSAPKHVQQFQKEGYLRWDSLGEFSAFAASLEHLAGVFQNK
                     KAKVLAETLDEAIAKFLENNKSPARKVGQIDNRGSHYYLALYWAQALAAQNKDPELRA
                     RFETIARRLGESEATIAAELLGAQAKPVDMGGYYRPDRKKVETAMRPSPTLNAIIDAL
                     "
     gene            30501..32501
                     /locus_tag="PKV64_02250"
     CDS             30501..32501
                     /locus_tag="PKV64_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN15934.1"
                     /translation="MKRIAKFFFASAGLICLVLPLSAQEKTLDELLALDLSELLDLKV
                     VSALKSPETIARTPATVRVITSEQIRDNGCLTLEDALADLPGFQFRNILGFNSYSFIR
                     GVPGQNNKILLLVDGLQINELNSGGFYGGGQFNLAEVERIEVIYGPASALYGTNALSG
                     IINIVTRDPRAGSRGRVSAGLGTFGARLADARYGYYDKNADFGFTAAAMLKRNEKADL
                     AGAAGDFNWTEAIENFENDAAVQARVHFKDFEAGFLLQDENASRATVEKTAGEDLSIP
                     WSDHGVNWHIRFINVWARYSYEKIKTWSFRNTAYYRNSTVLDDTVSEIELATSDSPGR
                     QFRCYRPNSLIGNETQVQWAPDPRWRFSAGLVLERERLAEGFSVTVSSSADALPAVPL
                     SPAMMTNRLFSAYAQIQGTPAKGLDLFLGLRHDDSSYYGAVNTPRLGVIYNRGKLTAK
                     ALYMRAFRAPKPWDYAAGAGNPGLRPEKIDSFEIAGGWSFSPFLRLDVSVYRNRLTNL
                     LSREDETTNSRWINAGALTTDGGEAGLLFRRGSWRAWAYYTYTASLDETGEQAPEIAA
                     HCATFGAAYAFTPRLTLSLRGCYFGERKNPKIIPSTGSDRIEGAFVARAALSWRLPAG
                     FDIQLVVDNLFDAVYFHPSNLPPSRFRQPPRSFRLAAGYSFQ"
     gene            32501..33085
                     /locus_tag="PKV64_02255"
     CDS             32501..33085
                     /locus_tag="PKV64_02255"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HPN15935.1"
                     /translation="MKTARFRIGWILAAAGAVFLSGPPAVPAGKTLVTDTSASEAAVK
                     AVFLYHFIRYLQWPGEFDREPCPIVVLGDSAIWEPLQEISEKKTNGSVLLLVRRCEEL
                     KDAGRPRILFLSDSSAFLLPEALEKFRGTSVLVVGETEGLARRGAAINFVLRDEHVKF
                     EINLDALKQAGIQAGSQLLRLALLVEGDKAAPGR"
     gene            33082..34968
                     /locus_tag="PKV64_02260"
     CDS             33082..34968
                     /locus_tag="PKV64_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN15936.1"
                     /translation="MKFNDLSLARKIIFLQLFVVFIVVFLLSGFNVLSSARFYRNSLR
                     TKLESMANLLGYNCTSALLFQDGVDAARTLRSLEAEALVTHAWILDAGGRPFAAYRRK
                     ETAAEPPPQRGGDYEETQGRFFVFSRRILQDDEPIGTVLFRYDLSEYRMILFRNALGT
                     GAALLASMLLALLLTARSQRTLSRPIRRLAETIRLVSRTKDLSIRVRGEEARKDEIGV
                     LHRGFNSMLSEIQSRDAERERMIAALRESEEKYRTLVENAEDAIVIIQDERFVYVNPS
                     LGAMARGQAGEFLGRPFLDVIPEEERERLTRYYRDRMAGRETPTMYETALMNAGGGRI
                     PAEVTAARISYQGRPADLVIVRDISERKKAEEEIRKLNEGLERRVEERTRELAAANER
                     LIELDRLKSLFLASMSHELRTPLNSILGFTGLILMGMSGDVSTEQRKQLTMVQSSATH
                     LLALINDILDISKIESGRMDLTIESFPAADVVRETVKSVTPQAEAKGLRVRVDAPDGL
                     FLQSDVRRFKQVLMNLLGNAIKFSLRGDIRVQADVREEYLEVRVADQGIGIRAEDLPK
                     LFVPFQQIDMTMTKAYEGTGLGLYLCKKIVTALRGGMTVSSEPGSGSVFVFRLPLRWK
                     EDPR"
     gene            34965..35324
                     /locus_tag="PKV64_02265"
     CDS             34965..35324
                     /locus_tag="PKV64_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN15937.1"
                     /translation="MKTVLIVEDNENNLYMMRFIIDKLGHAVIEARDGAAGVALAKER
                     RPDLILMDIQLPVMDGYAATRKIRADEALAGIPIIAVTSYAMVGDREKALAAGCTAYV
                     EKPIHPAAFIGVLEKYL"
     gene            35340..36482
                     /locus_tag="PKV64_02270"
     CDS             35340..36482
                     /locus_tag="PKV64_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN15938.1"
                     /translation="MKILIVDDEEKSRYLLARLFSGSGHAVEEAADGKIALEMLEKEA
                     YDLVISDILMPVMDGYALCRELRSREGLRETPFVFLTATYVDEKDERFALQLGADRFY
                     RKPVDPRTFVREIDLLLEEIRDGKQRRLGPETDEKEILTLYNERLINKLEKKIVALEN
                     EIAERKRAEDKARAAIEENEVLLREVHHRVKNNMQLVSSLINLSARGLKDPAALDVLR
                     QIRLRIRSISMIHEKLMKSGDLVRVDFADFLDDMAIHFFQFYKADPDRISLKLEVVPV
                     MLPVGLAVPCALIAGELMSNALKHAFPGGRRGKILVGLKPLESGEFLLRVSDDGVGFP
                     ETADIRKMETLGMTILNALVAQIGGRLEILRDGGSDFRITFARRPF"
     gene            complement(36522..36926)
                     /locus_tag="PKV64_02275"
     CDS             complement(36522..36926)
                     /locus_tag="PKV64_02275"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPN15939.1"
                     /translation="MSKEKSREDKVEKSLAVISEGASMDGVLQSTAVIHLCGSFHGRI
                     IAENELIIAAGGKVRADVRARKAVVSGDFEGEMAVTEEIEITETGRFAGTLFQRAPGL
                     VIARGGRFDGRSVFVEDLDAVAAGWEAGGKKG"
     gene            37272..40280
                     /locus_tag="PKV64_02280"
     CDS             37272..40280
                     /locus_tag="PKV64_02280"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPN15940.1"
                     /translation="MAFGFKGYGLDQGLTSPSITTMVQDLDGFLWVGTEDGLFRLEGK
                     RFRRFGTEDGLPANNIEALSLGRPRGIWATTSKGIVRWDGRRFLRPGGFGFPGFDNRP
                     GLAVPGGAIILSHFEAKQRFFSSTDEKSFSELKGLPWGGGANCAFFDAGRNFLLVSLQ
                     EGLWAWDGKAWKSRVLFDDAEGHKDIRMLLVDRAGRIWIRLTERLYRLESFEAPPVAV
                     TVPEPLSMVNHFFLVEDAFGRVWTNTARNLIWVSESGSGVLGERQGLPPTGAFVLYVD
                     VQGTLWAGGDGVFKLLGDSLWSWATRKEGLPADVVWSICRTRDGRVWAGTGGGLAFGT
                     PEGWRLVPGTGRCQILSLYEDAGGTLWVGHEPGGGRTTGLMSVPPGGTEAWTVPVFGI
                     ETRGPFFTMASDSKNSFWISAAEAGLLRAFPGPAGVRVEKFAVPGWIEEPFITKIDPD
                     GAGGIWVSSDQGAAHWNGRDWAVLPKGTLSDQEMMSVLALGSESAWFALSSGKRLVRV
                     RREGDALRLAELLPASHPLSQSLIFALAADPYGVIWAATARGLMRWEGGRVERYGRNA
                     GFPGEDCAQNALWVEPNGDVWAGLSVGLVHGAMNLRREPQTPPGVVLLESKDGQGRLL
                     DDADPGPTVSWPARTMAFRYALRGSRWTEGAGFQVRLVGMEEAWRNTDVSEALFPGLD
                     AGHYRFEVRAVSTTAETGAPESFSFRILAPWWRRWWSQLGGALILAGAVVLAFRRRTA
                     ILLRRNEFLESLVQARTSELEKANEALREAVLIDPLTGLYNRRYLTLTMPEEEIRLRR
                     MFRSYLQKGESPLNRNEDLVLFLGDLDFFKRINDEFGHAAGDQVIMETARVLRTASRT
                     ADTLVRWGGEEFLLVAKRTDRTKAHLIAEKLCRAVRDHVSVLPGGATTGCKITIGFAA
                     FPILEQNPEAFTWEDALQVADQCLYAVKRAGGDGWAGIHTPGPLDSVEILSRLRMDLA
                     GLVREGFIRVQSSFPEGSIFNGLGAPPKKY"
     gene            40362..40634
                     /locus_tag="PKV64_02285"
     CDS             40362..40634
                     /locus_tag="PKV64_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15941.1"
                     /translation="MSARAVFLGLALIAALANVAALIMIMAALDRRGRKTNMFMARLF
                     TGRYLSAYKETTKKEIGKPGPLYSLWLVAIILALVFAVIAVFLPQE"
     gene            complement(40690..41841)
                     /locus_tag="PKV64_02290"
     CDS             complement(40690..41841)
                     /locus_tag="PKV64_02290"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HPN15942.1"
                     /translation="MRILIATDGFKGSLTSIEAGAAVAAGLREGFARLGVPAAIDVVP
                     TADGGDGTVEAFYGAGGGRKISVTCPGPLGDPVSAEFLLRPGGKTAVIETARSSGLTL
                     VEGRRDILRSDTSGLGRQIEAALEHRPENVLIGLGGSATNDWGAGMAAALGARFLDGE
                     GRILPPRPAALSELRTVETAGLNPRLQAVRFEVLADVDNPLLGPHGAASVFAPQKGAD
                     AETVGRLEETGRRFADAVEAALGRKYRDLPGAGAAGGLGFGTAAFLGAVIRRGVETII
                     EATGFKEKARRADLVVTGEGRLDFQSRRGKTADGIARLARELKKPCVAFCGSIDGPLS
                     DYVPGLFAAAYAIRPEALSLEDAIRRGAHYLETVARRAAREIAALAAGS"
     gene            complement(41853..43166)
                     /gene="larA"
                     /locus_tag="PKV64_02295"
     CDS             complement(41853..43166)
                     /gene="larA"
                     /locus_tag="PKV64_02295"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HPN15943.1"
                     /translation="MNVRALSSREEILLRVPDDADLFVSRYPVPAAPAASLVLDAVQR
                     PLGAPPLREALGSRRPGEVVVVVSDVTRPVPYRDFLAPLLGEIAAAGVPQNDILILIA
                     AGMHRPSTTAERREMFGPDIVDRCRIEDHQAGDGPGLAAAPGRSASGAAIRLNRRFVE
                     AGFRLVTGLVEPHFMAGFSGGRKSVCPGLSSLETIREFHGEKMLSDPRAANAVLEGNP
                     LHEESLSVARAAGVDFTLNVVVDRGRRLVRAFAGALEPAHEAACAFVQTCAVRPVRRP
                     ADVVLTGSGGYPLDATFYQCVKGFVSCLPAVREGGTVIAFGGCSEGLGSPAYAGLLRE
                     YAGRFDDFLADIKRPGVFTVDQWELQMQARALARVGAGGLLFLSDGLPEETMRILTLT
                     GRTASRGRLGAAVQSALDTALGPGKTLAVFPEGPYCVPESIPSGE"
     gene            complement(43180..46434)
                     /locus_tag="PKV64_02300"
     CDS             complement(43180..46434)
                     /locus_tag="PKV64_02300"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPN15944.1"
                     /translation="MADEIPKLYEPSPHERRVTERWLAADAFAAVPDRRDRRYVIMMP
                     LPNVTGALHMGHAMDNVMQDLLIRWRRMAGDNTLWMPGTDHAGIATQAVVEKRLFELE
                     GKTRHDLGRDALVARIWAWKDEYQARIIRQQQRMGCSCDWKRQRFTMDAVCARAVREA
                     FFRLFADGLIYRGDRLVNWDCQLQTAVSDDEIVHEKVQGHFWHIRYPVIEPRAGEPEF
                     VVVATTRPETMLGDTAVAVHPDPAAELERRIAETAVKIAAAPKKDRAELEQTLENLES
                     RRRGVLPLLLRLRDMARDGRRLRLPLLDRPLPLIVDAWADPALGTGCVKITPAHDPND
                     YGVWERHKDEIGIINILNPDGTLNASAGPYAGVDRFDARRRVVADLKARNLLESVEDR
                     EMEVGHSDRSKTPIEPYLSKQWFVRMGDVDGGIVCGAGTPNEFRASGLAQAAIDASRP
                     GYKSPTGRRLTFAPDDDRYGGTYRAWLGEKRDWCISRQLWWGHRIPIWHGTFPGGKIA
                     EVAARLPQAGAEEMRVRIADDAGDSFEPGGRAALDPARNYSILVSLRNEDAETLHAPK
                     LEAAGLVQDPDVLDTWFSSALWPLSTLGWPDPATAVLDPGQRPLTPPEPGLDDCLDYY
                     YPGSCLVTSRDIITLWVARMMIMGLYLRGDVPFTASFIHATIQDGKGERMSKSKGNGI
                     DPEDIIEKYGTDAMRYVLCDMQTGTQDIRLPVQAVSPFTGELVDLAAAKHGRSIFTYI
                     DPRTGKEFDVLGTMPDLPAAKIISERFETGRAFCTKLWNAARFMFQNLGGHPFRPLSE
                     SDLAAEDRWILSRLSKVIRNVDEELQAYNPSAALGAAREFFWGELCDWYLEIIKPRMR
                     DEGAAPTARSVLALAFDHVLRLFHPFVPFITEALWERLNGQCPVRGLAAPLPPSPLLL
                     QARWPVPNAQWEDDALEADFGLAFDLVRAIRDMRARRGVSPALKLPVHVRTTEKDAAV
                     LARLRSFVIHLAHLESLDAGPAVVRPAAAAVQVAGGMEIFVPGLVDPVRERRQLTAKR
                     DKLIEEAGKIEAKLGNEGFVARAPAEVVEKERRKLAECRSQIEALEAGLRELSP"
     gene            complement(47098..>47288)
                     /locus_tag="PKV64_02305"
     CDS             complement(47098..>47288)
                     /locus_tag="PKV64_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HPN15945.1"
                     /translation="VLMKTLNIITISIMVPTLVVSIFSMNVTLPFGLKELPAFWIIMG
                     LAAISMTGFLAFWKYKRW"
BASE COUNT         8026 a        14624 c        15437 g         9001 t
ORIGIN      
        1 cggccgcggc cgggacgagc gggaaggacg gaggggaaat ccgggcgtcg cccgccgccg
       61 ggtaccgcca gatctttctc cttctgggac tcctgtatcc gccggaggac gtgggccggg
      121 cccttcagaa ttatgccgag ggcacgaagt gggccgtgga ttatgccctg gagcttctcg
      181 aggccgtcct cccgaaaaag atgaaggacg cggtcctgcc ggtgctggag gacgcgcccg
      241 ccgacgtgaa agcccggtcg gcccggcgga tcctgaaaac attgggatga ggaggaatcc
      301 atggccacgc tttatgtcta ccccaaaacg gccccgccgt tctccgccct cctcgaaaag
      361 gaagaaaccg ttctgggccg ggccttggac gcggatgttc gtcttccgga cgcctacatc
      421 tcgagccgtc atgcccggat ccggcggaca tcgcgaggct ggacggtgga ggatgcgggc
      481 agcaagaacg ggacgatggt caacggccgc cgcatcgtcg aacccgtcct tctcgcgccg
      541 ggcgatgaga tctccgtcgg ggcggtccgg atttgtttcg accggccgtg gacggcggcc
      601 gtcgaaatca ccgattcgcc caatcccgcc gtccgggtca gcgcggccgt tccttcccgg
      661 gacatcatca cccggagcac ggaagccggc gtcagacgga tggagccctc cgggccggga
      721 acgcgggccg accagcttct tctcgtcatc aacgaggtca gccgggctct tcttttccac
      781 aagcctcttc aggagctctt ggaatatgtc atggatctga tcgcctccca cctccccatg
      841 gaccgggggg ccttgttcct caaggagggg gatccggaga aacttgtccc ccgggccgtc
      901 cggatcggca atccccagtt gaaaagcggg ccgatgcaga tcagccgcgg cctcgtggaa
      961 atggcttctt cccggcggct ttcggtcctg acgaccgacg cggccgagga cccgcggttc
     1021 gccgaccgtc agagcgtcat cgacatgggg ctgcattcgg cgatgtgcgt ccccttggag
     1081 acggagaagg agattttggg cgtcgtctgc gccgagcgcg tttcgagccg ggaatcgttc
     1141 accgacgagg acctccggct gttgacgctg atggccaatc tggccgcggt caagatcgaa
     1201 aacgcccgcc tggtcgagga cggcctgatc cgggaccgga tggagcggga gctgcgcttg
     1261 gccgcggaaa tccagcgggg actcctgccg aaggaatgcc cggtctgcgg cgatttcgag
     1321 atcatcgccg aatccacgcc gtcactccac gtcggcggcg attatttcga tttcatcccg
     1381 ctggccgaca ggcggctggg aacgaccgtg gccgacgtcg ccggcaaggg cgtcagcgct
     1441 tcgctcctca tggcgtccct ccgggcggcc ctccaatcgg aagcccggcc cggaatgggt
     1501 ccggccgatc tcgccttcaa gctcaacaat ttcattcatc gcagctccgc ccccaatgtg
     1561 tttatcacgt ttttctacgg cgagctcgat ccatccacgg gcgatttccg ctacgtcaac
     1621 gccggtcaca atccgccgct tcttttgaaa gcgggcggaa aagagctttc cttggagggg
     1681 acgggcatgg ccttgggcat gctccctgaa agcacctacg aggaaggccg ggcggacttg
     1741 gaggccgggg acatcctcgt tctctacacc gacggcattt ccgaatgccg cagcccggcc
     1801 ggcgaggaat ttggaacggg ccggatcgtc cgggcggtcc gccgcttggc cggccggccg
     1861 gccggggaaa tcctgggagc gatcttttcc gacctgcgcg aattcaccgg cggggccgat
     1921 cccgacgacg accggacggt cttaatcatc aagaggccgg cggagcgggc gtcctgaacg
     1981 cccgctttcc ccgattcgct ttttcccccg gatttcctta tgattacgcc ttgtttacgg
     2041 atgaatcacg agccgagagg acggccgttt cctaaaaacc acgcggcttc ggggaggcgg
     2101 accgccgggc cggccgttga tgaatccgtt cggcttgatg gaaggcggct tccaaggatg
     2161 ttgatgaaga aggaaagctt gtcgatccgc ggggaaaacg gatgaaaacg aggatcgtcc
     2221 tcatcgcggc cgccggggcg gcgcttctcg gcgtcttctc tctggcgggc gcggacgtcg
     2281 agtcttcccg gcggaacgac gggccccccg tttccgccgc gccggcggaa accgtcctcc
     2341 gggccgaccc ggtcttcgac gatctcgcct ctttcctggc cggctttccc tgcgtctcct
     2401 ctccctataa ggaattccag gagaccgcgg agtaccggga gttcgccgac gccctggaaa
     2461 agaattgggc ggagctggaa atgaaacggc tgggtccggt ccggcattgg gcccgatcgg
     2521 agatcggcga ggcggccgcg gccacgacga cgctgtttta tcctttcggc ggccccgatg
     2581 ccttgacggc cgtgattctc tttccccagg ccgcccggca tcttctgctc ggcttggagt
     2641 ttgtcggccg gctgcccgtc tttacggccg atcaaggcgg cgccgccgcc cggtacatcc
     2701 aggatctccg ggcttcgctc gacgattttt tcaagaaaag ctatttcatc acccggaaca
     2761 tgaacgacga gctggccggc gataaggtcg acggcgtcct gccgctttta tgcttcttcc
     2821 tgaaacgcag cggcaacgtc atcgcctcga tcacccggct ggacatcacc gatcagggcg
     2881 gaatcctgga aggccccttt cccggcgagc cgaaaaagcg ccgccggccg ttcggcgtcc
     2941 gactcgcgta tttcgctgag ggagccgacg tcctgaaaga gctgtcttat atttcctgcg
     3001 acttgtccaa cggggcgttc cgcgagaatt ccccgctggc tttctacctt gggacggtgg
     3061 cctttgagac gaccttcctt aaatccgcgt cctatttgat gcattaccgt gatttctccc
     3121 ggatccgcgt tctgatcctg gaacggagcc ggttcgtcct tcaggacgac accggaatcc
     3181 ccttccggta cttcaaaccc ggccgctgga cggcccgtct ttacggcgaa tacagcgatc
     3241 cggtcaagga tttcgccaac gtcggccagc ccgatttgaa gaaagctttc gccgatcccg
     3301 cgcgggtgaa atccctgccc ttccgcctgg gctaccactg ggggagcgac aagggcttgc
     3361 tgctttattt catgagaaaa ggatgaagac gaagttcagc ttgacgacgg ccaccgcctt
     3421 ggtcgtggcc aatatggtgg gctcgggcgt cttcaccagc cttggtttcc aggcggccgg
     3481 gatttcctcg ggcccggccc tcctgcttct ctggatcctg gggggggcga tcgctctgtt
     3541 cggggcgctg acgtattcgg aggcgggcgt cctttttccg cggagcggag gggaatacca
     3601 ttttctgacg gagatgttcc atcccgcctt gggttttctg gccggctgga tttcgttcct
     3661 cgtgggattc gcggcccccg tggccgccgc ggcgatgggg ctgggccgct acctgacgag
     3721 cgccttggcc gtcccccaaa aaacgggagc cggcttgtcc tcttcctcgt tgatcgccgt
     3781 cggcgtcgtc gccgtcctga ccgtcctcca cagcctggac aagagccttg gcgcccggtt
     3841 tcagaacgtc atcacgattt tcaagatcct tttcattgtc ctgatcatcg tcctcggcct
     3901 ggcgatgggc gagccggccg gcctgtcctt cgccccgacg agccgggcgc tccgggacgt
     3961 tctcagcccg gccttcgccg tctcgatgtt cttcgtgacg ttcagctatt ccggctggaa
     4021 cgccgcggct tatgtcgccg gcgagatcga gaatccgcgg cggaacctcc cccggagcct
     4081 gatcgccggg accctgatcg tcagcgtcct ctatatcctt ctgaatttcg tctttctccg
     4141 gaccgtcccg atcgccgagc tgtcgggaaa gctggaagtt ggatacgtgt tcgccggccg
     4201 cgtgttcggc gcggccggcg gccggttgat gggcgcggtc atcgccttcc ttcttctttc
     4261 gagcgtcagc gcgatgatca tcgccgggcc gcggatctcc cgggtcatcg gcgaggatta
     4321 ccgcctgttc cggttcctgg ggaagacgac ggccaaggac atcccggcgg cggccgtcgt
     4381 cgtccagggg ctgttgtccg ttttctacat cgtcacctcg acgttcgacc aagtgatcgt
     4441 tttcatcgga ttcaccctga atctcttcac attcatgacc gtgctggggg tgatgctcat
     4501 gcggcggaag cggccggacc tgccgcgtcc ctaccggacc tggggatacc cctggacgcc
     4561 ggccgccttt ctcgtcatcc acgtttacat cctggcctac gggttgatct accggccgag
     4621 agaatcgctg gccggactcg ggttgacggc cgcaggtctc gtcgtctatg ccatcgatcg
     4681 gaaaacgcgc cgggtcaagc cggattccgt ttgatggagg ttctcccgcc gtgttttttg
     4741 aagaaccgtc ggccggatca ggcgaaaaaa agattccgcg gcgggccgga gggcgcttct
     4801 tcgtaccgcg ccgtcgctca gccgaacaga tcggccgggg cgtcctggat ccgggcgatg
     4861 tccgtcgccc ggccgcgcac gtcgatcccc agcgcgcact gaaccgggca ggatgcgcag
     4921 tcggcgcagg gattccgatc cacgccgagg gaggcgacga ggtcgtaggc ttctccgagg
     4981 ttgcgatagc cgtaggcgta catgtagccc cgcatcaagg aaggaagcgg taagcgccgg
     5041 gcgcattggg aaaggcaggt ctggcatcct tggcaataga gccccggctc ccccccgccg
     5101 ccgagctcca agtcggcttt ttccgcgtcc gtcaagacga tgtcggtcat agcctggagg
     5161 ttgagggcga gctggtcgaa ggtcgtcatg ccgggaatcg ccgtggtgat atggggattg
     5221 cccagggccc atttcaaggc ggccttcatg ttgatcggac gggtttttcc ccggtcccag
     5281 tacgcgccgg cctgggtctt catggcgatg atcccgattc cgccggccgc ggcatcggcc
     5341 acggccgcgt cgagcgcgac ccggttggtc tgccggaaat tgtaggacgt caggacgacg
     5401 tcgtggatct tgccctcgat cgccgcccgg atcacttcgt gttcgttgcc gtgggttgag
     5461 acgccgatga agcgggcctt gccgctttgc ttgatcttga cgagggcgtt catcagaggc
     5521 tcgaacagga cggcctcctt cgtcagaacg ttatggagat agaggatgtc gacgtattcc
     5581 accccgaggc gccggaggct gagttcgaat ttctcgagaa acggcccggc cgtcgtttcg
     5641 gccaagaaat tgcccgtccg gcggtcctgc ggctcgccgg ggaccttggt tgcgatgaaa
     5701 taggactcgc gcgggcggcc cttgaggact ttcccgatca cgccttcgtt cgttccgcgc
     5761 tgatagccgt gggcggtgtc caggtgaacg attcccccgt cgagggcggc ctggatcagg
     5821 ttttcgttgt tggagttcat cacgcccatc gaaaccaccg gaagcgcgac gcccgttttt
     5881 ccgagagggc gggtgatgac gccgggcttg gccgcggccg gggcggagga tgcttgaacg
     5941 ccggctttca gaacgccggg aacggcgccc caagcgccgg ctccggcgac tccgagcagg
     6001 ctttttttca agaattcgcg gcggtcgaaa gatgagcgct tggcgggcat cggcgtcctc
     6061 cttgaaggat gtcgaaaaga tgagcggata ctataacatg ccgccttttt caggtccaga
     6121 atacgaatcg cgccgtctga aatccaatcc cggccggggg ggtgaaagga gatgattcga
     6181 tccggcttcg gtcaggattt ttaatggcaa gacatgatcc tctcgggcaa gatgatgaat
     6241 ggatccaggg ccatccgccg gcgtttttcg ggtataatgc ccccatgacg atcgatcaaa
     6301 atcctccttc gattgggcgc aggtgggtga cggccgcccg gccttgggcg tttcccgctt
     6361 cgacgatgcc ggtgatcttc ggaacgagcc tggccgtcgt cgccggcggg gcgtccttga
     6421 acatcctcct gttcggcggg gcgttcgccg cgatggtgat tctccacgcg gcggccaaca
     6481 tgctgagcga tgtcttcgac ttccggcacg gagtggatac ccaagcgacg ccggtcagcg
     6541 gagcgatcgt ccgcggctgg ctcaccgagc gccaggtcgc ccggggggcc gtcgccctgt
     6601 tcgccgccgg catcgccctg ggcctggtct tggcatggcg gacgagctgg atccttttgg
     6661 cgatcggcgc ggccggggtt ctcatcggag cggcgtacac cttgttgaaa gcccgggctc
     6721 tcggggatgc ggccgtgttt ctcaatttcg gcgtgttggg ggcgctgggc gcctgggtcg
     6781 tccagaccgg gcggttttcc tggctgccgg tcgcctggag cgttccgatc gccatgcttg
     6841 tcagcgggat tctccacgcc aacaactggc gcgacatcgc cacggattcc ggccgccgga
     6901 tccggacctg cgccggaatc ctcggcgacc gcggctcgat cgtctactat ggattcctct
     6961 tgttcgggag tcaactcctg gtcatcgctt tcgccggctt cccgctcgtc cttcccggtc
     7021 tgccccggct tccttgcccg tgcctgatca cgctggccgt cctccccgag gccgcgaccc
     7081 tctggcggcg ggcccggcgc cggcaccggc cgaaaaatcc cttggacttc gttgtcttgg
     7141 atggggcgac ggcccgctat aatcttcact tcggccttct ctacacggcc gggttatggc
     7201 tcggccgttg gctgggatga tccccccaaa tggagcgccg cttggccggc tcgcttgaac
     7261 ggtccgggtg aatcgtcgat gggaagccgg aaagcaaggg cggcggccga ttttgccccg
     7321 gccggagcgg ccgtcctctt ttttgttcct cttttcattt tcaaaggttt cggcccgttg
     7381 gatttctggc aggggatggc cggatccttg gtcctgctcg tcgttctcgg cgcggccctc
     7441 gaccgggatt ttgcgtccct gctttgggcc gatgttcgga acgaatcggg ccgcaagatc
     7501 cttcggggcg tggtttcggc ggtttttctt tatgccgtct tttgggccgg aaacgcggtc
     7561 tcccgggtcc ttttttcctt tgccgaaggc gggatttccg aggtctacgg cttcaaggac
     7621 ggagcttcga cgttcaggat cggctttctc ctgggcctca tcatcggtcc gggggaggag
     7681 ctgttctggc gtggatttct ccagcggcgc tgggcggcct ggtttgggcc gggccggggc
     7741 tttctcgccg cgacggtgct ttatgcggcc gttcatgccg gctcggggaa tccgatgctg
     7801 atttccgcgg ccgcggtctg cggagttttc tggggatttt tatatctgcg gaccggctcg
     7861 gcgcttcttt gcgccgtgag ccacactctc tgggacttgg ccgtcttcct cgttttcccg
     7921 ttcgcctgag cttcccttca atccgggtcg cctcacttca ttccaataaa tcggaaacag
     7981 gttctttatc tcccgatttt cgtcggtgga atccgagagc cagcggacct cgcctcgcgg
     8041 cttttccggc ggatgagaat catggaatcg ccccgccgtc aaccggcggc cccgccgccg
     8101 gttgacggcg acggagcgtc acaacggacg gacgggctcg tggatctcga tgatgaattt
     8161 cccctcggga tgggttttcg ggtcgttgag ataaagctcg tagcacatcc ggtcgtccgg
     8221 ctcatacccg cttcccggca gccagtcgcg catcaaggcg ttccaggcct cgccgaattc
     8281 gtccggggag acctcggccc gggcgacggc gaacagtccg ccggggacgg tcatggcgcc
     8341 gatttctccc ccgacgttcg tcccgggagg gacggtgatg caggcgtcgg agcggagctt
     8401 gtcgtttgcg gtgatgtccg gatcgtcatg ataaatgccc agcgtttttg tctccgggaa
     8461 gcggaggagg ccgcgcggac cggcccatcg catcagcttt tcgaacgcct gggacattcc
     8521 ttggaacgga ccgacatgcc ggacataggc gaccgtcatc tccggcgcct ccctgacctc
     8581 gaccttgaat ttcgacttgt ccatgaagga cctcctgaat gaaatgagag cgtcctcatc
     8641 atcatccagg gcgccgaaaa tctcttggct tatcttgcct tccgcttgac gattcttgcc
     8701 attcgtttgg cgtttcttgc gccaggcctc gcctccgccg gcccgccatt ccgaggcgct
     8761 catcccgaag cgctcccgga acgcccgggc gaaaacgtcc gagcccgagt agccgtagtc
     8821 cagggcgatt tccgtgatgg tcgctttcgg gtgaaggagg agggccgcgg ccgccagctc
     8881 ggccctcctc cgctggatat aagcgttgag cgtctctccc gtcagggcgc tgaaaatccg
     8941 gtgaaaatga aaacgggaga aatgggccgt ccgggcgagc tcgtcgaggc ggaggtcccg
     9001 gcggatgttg aggtcgatgt agtcgaggac ccggttgatg cgggcccggt actcggctcg
     9061 aagcgcttcg gtccggtcaa gcggcatgcg gcatcctttt tcgattcgtc agggaggcga
     9121 aacggcctcc ccgtgctccg gaatggtacc atccctccgg agagcaggac aagcggatcc
     9181 gcctcgctcc gtcccaaaaa tcagcgaagg gagggagggg cgcgccccca tccgccgaac
     9241 ggctgtgcta taattccgcc tgattccgcc gttttcggag agatatcgca tgaagcgtct
     9301 ttttttcatt cttggcattt tattggcgat cggcccggcc gtcttcagtc aagccccgga
     9361 cgatatcgtc tcggtcgaag tcgtcgctcc ggccggaggc ttcaccgccg gccgcaccgt
     9421 ccaagccgcg ctgaaattga ccatccggcc tccttttcac atcaactccg accggcccgc
     9481 ggacgaggac ctcatcggaa cgaccatcgt tttccggtct caggccgggg tgacatacgg
     9541 ctggccggcc tttccccggc cggtcaggag gaaactcagc ttctccgaaa cgccgctttc
     9601 cgttttcgag ggcgtgatca ccgttccgtt cgacgtcacg gtggccgcgg atttcgccgg
     9661 aaaggaatgg acgatcgccg gtacggtgag ctaccaggcc tgcgacgacc agacgtgtct
     9721 tccgccccgg gacgttccgt tttccgatac cgtcggcgtg gccttccccc cggcggcggc
     9781 tccgtccccg tcgccgccgg cggacaaaca ggcggcgtcg acggccgagg ctccggtccg
     9841 agcggaagaa ccgccggcgg ccgaagccgc cgactctgtt tctcctgaag cgaagccgga
     9901 atccggaccg ccggcgcttg aacccgcggc cgggccttcc ggccgggaag tcccgtttga
     9961 agggaaaggg ctcccggtca ttttcgccct tgtcttcctg ggcgggctgg gcctgaccct
    10021 gactccctgc gtctatccca tcatcccgat cacgatcagc tatttcggcg gccaatccga
    10081 ggggaaaaag ggcggcgtct tcctccacgc gatcctgtac gttttaggca tggccgtcac
    10141 gtattcgatt ctcggggtga tcgcggcctt caccggggga ttgttcggcg cggccttgac
    10201 gtatcctccc gtcctgatcg ggatcgccct gatcatgatc gccctgtcgc tcagcatgtt
    10261 cggcgtctac gaattccgca tgcccgcttt tctcaacacg ctggccggag gaagccaaaa
    10321 aggatatttc ggcacggtcc tcatgggctt gaccgtcggg atcgtcgccg ccccctgcat
    10381 cgggccgttc gtcctggggc ttctcacgta cgtcggcaac cggggcgatg tcttgctcgg
    10441 cttctccttg ttcttcgtcc tggccctcgg cctgggcgtc cccttcctta tcctgggggt
    10501 cttttccggc gccctgggcc ggcttccccg ctcgggcgcc tggatgatct gggtccggaa
    10561 gattttcggg ttcatcctca tcgccatggc cgcctatttc ctgaagaccc tgttcccgaa
    10621 cacgcttttc taccatttcg tcctggccct gatcatgctg gtcgccggcc tctatctcgg
    10681 ctggatcgag ccgacgaaga cgggcgggaa ggcgttttcg tacgtccggg ccctgacagg
    10741 cgtcgtcttc ctcgttctcg ccctcctttt cgccgtccgg ggcgtggagg aatacgtcgg
    10801 cgggacggcc tcggccggcg ccgccgccgg cctcgtttcg gaagccatcg cctggcggcc
    10861 gtacagcgag gaagccctcg cggccgcgtc cgcggcggga aagccggtgt tcatcgacgg
    10921 gttcgccgat tggtgcatcc cctgcaagga gctggaccgg atgaccttca gccggccgga
    10981 cgtcgtcgcg gcctcccgga gcttcgtcat gctcaaggcc gacctgaccg ccgggggaga
    11041 cgagaaggtg aaggcttttt ctcagaaata cggcattcgc ggcgtcccca ccctcctctt
    11101 tctccgtccg gacgggacgg aaatcgagga gctgcgggga acgggttttg aacccgccga
    11161 ggcctttctc gcccggatga ggaaagccct ggagcggagc ggggaaaagg cggccggccg
    11221 gtgaaaatcg aaggcgttcc cttcaccgtg accgactggg gctccgtccc ggctgtggag
    11281 cacaagggcg cctcggggac gtcgttctgg cggacggttg aagccggcaa cctccgggtc
    11341 cggatggtgg cttattcccc gggcttcgcc tccgatcact ggtgcgcccg cggccacgct
    11401 ctcaccgtcc tggacgggga aatcacggtc gccctgaagg acggccggga attcgtcctg
    11461 accgcgggca tgagcttcca ggcggaagac gacgaagctt gtccccactt ggcccgctcg
    11521 ggcttcgggg cccgggtcct catcgtcgac tagcacgagc tctccgtttc tccttctcaa
    11581 ggcgttccgc cgggacccag tcctgagaac gtcttggcca cttcgatgaa gtgctggatg
    11641 acttccggat cgaattgcga cccggagcag cggatgatct ccttgacggc gaaatggtgg
    11701 gattcctcgc cgcggtacgg ccgcttcgag gtgatcgcgt cgtaagagtc ggccacggtc
    11761 atgatcctcg cccccaaggg gatttccagg cctttcaggc cgtccggata tcccttgccg
    11821 tcgtaccgtt cgtggtggca ccgaacgatc ttggcgatct gggtcagctc gacgacgggc
    11881 ttcaggatgc tttcgccgaa aacgggatga tccttgatcg ccttgtattc ctcgccggtc
    11941 agcggcccgg gcttgttgag gatgttttcc gggatgccga tcttgcccac gtcgtgaagg
    12001 aggccggcca attcgaggtc gtccagctcg cgttcgccga agcccatgtg gcgggcgatc
    12061 ttcagggaga attccgtaac ccgggccgaa tggtgcctcg tgtagaaatc cttcgcctcg
    12121 agggccgaga cgagcgactt gaccatcgag atgaagagca tccgctgccg ttcgacgagg
    12181 tcgctggtcc gcatcttcag gttgcggttg gccagggcca gctcggcctc aacctgccgc
    12241 atccggaggt gggaccggat ccgggctttg agctcgacgg cgttgaacgg cttgacgatg
    12301 taatcgtcgg ccccggactc catgcccgcg gccagcattt cagaggccga ccgggcggtc
    12361 accagaagga cgggcgtgtg cctgaccgcc tcatcggact tgatcctccg gcagagctca
    12421 tggccgtcca tcccgggcat catgacgtcg ctgaggacga ggtccggtcc gcgctcatga
    12481 agaatccgca ggccgtcctc tccggtcagg gcgaagtcca ggtcgaattc atccttgagc
    12541 agcaagtgca tcagcttgag aacctcgggg ctgtcgtcga tcaccagaag ctggtactcg
    12601 tggcggcgct ccgccggcgg ggtgatcgga aattcgactt tttccaagtc gatgagctgc
    12661 aaatcccgga cattggagat gatgtcctgg acacggacgc cttccggctc gatgtcggtc
    12721 gcccgtttcc ggaaggcgac gggctgatcg gactggcggc ggtccatgac ctcctcagag
    12781 aagtgcctgg ttcccttgag gagctcgacg atgaaccggg agccctcgcc cggctcgctt
    12841 tcgacccgga tcaatccgcc gtggagggag acgacctcct tggccaaggc caggccgatt
    12901 cccgtccccg cccgggcggc cgatccctcc acctgccggt accggtcgaa cacggcctcg
    12961 agcatgtgac tcggaaggcc ggagccggtg tcctccaccg tgaggagaac ggtttccggg
    13021 cgctcctcga cgtacagggt gacccggccg ttttccgggg tgaatttcaa ggcgttggag
    13081 agaaggttga gaacgacctt ctcgaactgg tcgggatcga tcgtcagctc gacttcggtc
    13141 aagggatgct ggaaaaacag gcggatgttc ttttggtcgg ccaggacctt gaccgaggcg
    13201 aggagggttt cgacgtaatc gttgaattcc aaggttttcg gccggagccg catccgcccg
    13261 gcttccagct tgttgagatc caggaggttg ttgatgagct tgagcaggcg gtacccgttg
    13321 cggcggatgg aatccagcgt ttctttctga aggtcggtga cggnnnnnnn nnnnnnnnnn
    13381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13441 nnnnnnnnnn nnnnnnnnnn nnnccggagg ccggcggccg gaaaatcgag gggacggcgg
    13501 tgccgagcca gatcagggcg tacaaggacg ccgaggcggc gaagcgcagg ggaacgagga
    13561 cgacgacgcc catgatgacc aggtataaac cctggacgta ggccgtcgag actccgccca
    13621 gcatctggat catgatcgag atcccgccgg cgtcgaggac cgtcagggcg atcgcgacca
    13681 tctcggcccc gcggttggat cgcgtccgcc tcaggatgag caacagcagg ccatggccgg
    13741 cgagaacggc cagacggatc aagaggaacg tgtgaaacat cggcgggacg tacaagcggt
    13801 caaggaggat gaacgccgcg aagaggatgg cgctgaagaa aatgacccac tgggtgcgcc
    13861 cgcgcaggtc gtattcctcg cccagccgct cgaagaggct cttatccttg ttcctcatgt
    13921 cagttcctcg gtttggcgac gacgacgtat ccctgggggg gattcccgaa ggaaggcaga
    13981 atctcgacct gagtccagcc cgcttctccc aagagaagct ccagcttgcc gggatcgaag
    14041 aaatcgaatg tcccggcttc ctccagatcc acgagagcct gggcatcgtt gagaaacgac
    14101 cgcatcgatt cgaggatccg gcttttcgga ccggggatgg ggagcgcgtc ctccggggcc
    14161 gcttcgatct tttccaaaag ccgggtgaac aagccggacg cgtcggtatc cggccgcatg
    14221 ctcgacagga cgaggcgccc cccgggggcg atgaggcggc ggacttcccg gagggtttcg
    14281 accgggttga aaatatagga gaggacgagg ctcatcagga tcttgtcgaa tcgctcttcc
    14341 ttgaacggca gaaagaaagc ggtttcccgg ccgccgggga aggccagctt gcggaaagcc
    14401 ggatgccccg gggcaagacc ggcggtccag cgggcggcca gattgaagtc gcggatgacg
    14461 cggccttcgg ccagctcgaa ggtgcttttc agaaagcgct ccgtctcggg agtgatttcc
    14521 tcgccccgga ggatccggtg aaggcgggga gagtaggccg cgtcgatccg gagggcggat
    14581 tggaggggca ggttttcgat ctgctcggcc aagtcctcga accgcccgat ttcgccggcg
    14641 aggaacctcc ggatgggtat caatcggttc aattcgacgt cgaggcccag gacggacagc
    14701 cggccggcgt tcttcagcga gggctcgagg gaggtcagct tgtcgaaagc ccgggccagc
    14761 gcctccggga ccaggtcggc cagaaccaga cgcgagggca gggggcctcc gctttggagg
    14821 atgtgggcga tgaaattccc cgtgccgccc cccaggtcga ggaaccggtc ctccggatcg
    14881 aaagccaagg ccttgcgctg atcctccatg agccggacgt aatcgtccga caaggccatc
    14941 gtgtcgaagc cgagaagacc tttttccccc accaagtaat gggtccagta ggtgtgcttg
    15001 tttttcaaaa tccggcccgg gagacggtcc ttctccgccc gcctcagata ttcgagattc
    15061 ttccgctccg gcgggatcgc ctcgatcatc gtcccgtgaa ggaagcgatg gacgagggag
    15121 gtgaccgtcc cgaacagccg cagggcttcc tcggagttcc gggcgttatg tcccagggga
    15181 acggaaaaga cttcccggga agcctcggcc ttgatgctca tgacgtcgcg gacgaactcg
    15241 gccttgaccc aattatcgtg ttttccgtag atccaggtga ccgggaggtt gaggtggcgc
    15301 atgtcttccc gggcttggtc gagggtcgct accccggagg cgaccacgtc ggccgcgtaa
    15361 tgcctcattt tgaccaagtt gccgaggatg ggaatgaccc cgagatcgat gcccagctgg
    15421 tgctgctcga gcaggtcgag cccgcagttg acgcggttca tcaggtcgcg gaattccagc
    15481 gtgcccatgc aggcgatcca ataatggacg cgccgccgca cggcgggatc gcggagggcg
    15541 atccgggcct ccaaggcgga aagcgagaac gtcgccagga tcaccggacc ggccttgatc
    15601 gtcggattca tctccagcca ttccaggacc gttttcaagt ccgccgctcc ctggctgagg
    15661 ctggagtcga ccatttcgaa cggcggctcg gccgcctcgg gatccttgtg gctttcgcct
    15721 ttgcaccgga ttccgtcgta gcggaaaacc gccagccgcc ggccggcccg gcggaaattt
    15781 tcgactaggg tcagggccag gccgaacaat gtttccttcg tcttcccgaa agcgggcggg
    15841 atgatcacga cgggcacggg atttccgtcc ggcgggtacg atgtattcaa gaggccgacg
    15901 atttcttccc cccgctcgtt ttccaggcgg atgacggcgg gaggacgatg agaaagctcg
    15961 cggagaatgt ccggcagccc gggatcggcc ccgcttcccg gctcggcggc cgcggcgggg
    16021 gggacgtcgg tgctgcgccg atgggggtgg accgcctgga tgctcaagcg gccgatgccg
    16081 aactgaagcc catagcggaa ggccggggat ccgtcctctt ccgtccggac ggcgtggcgg
    16141 atttcgcccc gctcttcccg gatcaagcgg ccgttgtcga agatccggac ggtgccgacg
    16201 ggcgtgccgg gaagcagggc caagccgccg gcatcggcga tgaagctggc cccggtatcc
    16261 gagaggtcga cgaccggacc cgagatcccg gccgaaaatg ggggcgggag aaccagttcc
    16321 aggcggagac cgtcttgggg cgattccctc ttcaaggagc gtttttccga gtagaagagg
    16381 atccggggat agaagcactc gaggaaatcg ccgttctcgc cgaggcgccg gaccgttgtt
    16441 tcgaaattat acgtggcata tccggattgg aaggacatga agacgggatc cccggtcttg
    16501 aaatggaggt tgaggctttt tccgctcagg acgcagattc tcccggggtc gacgtgttcg
    16561 agagtgaggg tcgccggctg tcggacatgg gcgggaaggg cggtgatttc cgtccgggcc
    16621 cgccgggcac cggcgagcag cccccggatt ttatccggag aggagaaggc gaccatggcc
    16681 gaggtgtgaa gctccggata gcgcagcttg agctcggcca agtccttggg ggaaacggtt
    16741 tcggccaggc gggagaagat tttccggcgg gcctcctcgt cggccgggcg gaaggcgatg
    16801 ccgacgcctc cctcgccctc tccggagcgg acgacgattc cctcgacttt ccagcattcg
    16861 ccgtcggcga cgtgaaggac gaatggacgc ctcgccccga cgcccaggcc ggccgccgtt
    16921 tggagaaaca tgcctgtttc gctcaggtcg cgcacgacgg ccggtccgcc gccgccctcg
    16981 atttcgacgc ggatgggatt tttctcggaa atgggaatgc ggacaacggc cctcttttcc
    17041 acggccagcc gggacaagcg cttccggacc tccagacaga gagcttccgt ttcgaccggg
    17101 ctctcgagaa cgaggtcgga gggcgagatc aggagcgacc ggcgaagggc cgcggcctcc
    17161 tccggtccgg cgaccacgat cttgaccatc cgttccgtgc tctggaacga ttggagaagg
    17221 gaccggagaa acgcccccgg ctcggcggag atggaagccg ggtcacggag gatcaaacaa
    17281 tcgaccgtct ggagcttctc cgcccagtaa aaagcctcca acggaccgat cgcggcgatc
    17341 agatcggcgt tcccgttcaa cgcttttttg aaggaatgaa ggcggccggg gcggccgtcg
    17401 acggcgagga ctctcagacg gggggatgtc gtagagtctt tcggccggat ttcaggcatc
    17461 acgttttcca gggcggggga attgacgggg agtcaagtga atgaaatcgg atgaatgagc
    17521 ccctatgtcc acaaaaccta gggtccaaca tgttaatata cactattttg tgtggatttc
    17581 tatgttttgt caagaggaga atcgcgacgg gcgtgatttg ctccgtgtca aggagacggg
    17641 cggggccggc gggaaaaaag ggggaaggcc cgaaggcctt cccccggggg aaagcggtcg
    17701 cgcgattcag cttttcaaaa gaccgcgggc tttgagcagg gcttcgactt tcggctcgcg
    17761 cccccggaaa gccttgtagg aatccatcgc ttcccgggtg ttgcccttgg agaggatgtt
    17821 ctcccggaag cttttggccg tcttcggatc aaaaaggctc gtctccttga atgcctggaa
    17881 cgcgtccttg tccaggacct cgctccagag gtagctgtag tatccggccg aatatccgcc
    17941 cgagaagata tgggcaaaat acggggtccg gtaccggacg atgatttcgg ggatcattcc
    18001 gatctttttg agaagggcgt cctcgaaagc gacggcgtcc tggggggcgg cctcggccag
    18061 cgtatgccag gccatgtcga ggtaacaggc ggattcgaac tcgacggtcg tgaagccctg
    18121 gttgaactgc ccggtttttt cgatcttatc cagaagatcc aggggaatcg gctcgcctgt
    18181 ccggtagtgc ttggcatagg tcttgatcac ctcgggatcg gccgcccagt tctccatgat
    18241 ctgggagcag agctcgacga aatcgcgggg gacgttcgtc ccgccgatcg actcgtaggt
    18301 cacgtcggac aagaggtcct ggagggcgtg gccgaattca tggaaaagcg tcagggcttc
    18361 ctcgaaggtg atcagcgacg gctggtcggc ggtcggcttg gtgaaattgc cgacgttgta
    18421 gacgaccggc gtgacgcgcc ggccgtcttg gaccgactgc atccggatcg cgctcgacca
    18481 ggccccgccc cgcttggacg gacgcgggaa gtagtccgaa tagagaatgc cgacgtggcg
    18541 cccgtcggct tccttgacct cgaagacctc gacgtccgga tggtagaccg ggatgtcggt
    18601 ccggggcgtg aaggtcagtc cccagagctt gccggccgtg tcgaaggcgc cccggaggac
    18661 ggccttgagc tcgaaatacg gccgcaaggc ctcgtcgtcg aggtcgtact tggctttttt
    18721 cagctgatcg gcgtagtacc accagtccca gggctcgagc tttccaacga ctccgtcctt
    18781 gttcatcaac gcctcgagct cccgggcttc cttttccgcc atctccaaag ccggcgtcca
    18841 aacctggtcg aggagcttgt agacgccggc gggcgtcttg gccatgttca cctcgaggac
    18901 gtaatcggcg tgggtcgggt agccgagaag gttggcttgg atcacccgca gggcggcgat
    18961 ttttgaagcg atggcccggt tgtcggccgc gttgccgttg gctccccggc tgatgaaggc
    19021 cttgaagagc ttttcccgca ggtcgcggcg ggaggaatat tggaaaaagg gaatgcagga
    19081 cggcttgtgg agggagaaca cccagcggcc ttcctgtccc tgctccttgg cggcctcggc
    19141 cgctccggcg atgacgcctt ccggaagtcc ggccaagtcc tccttcttgt cgagaacgag
    19201 ggtgaagccg ttgtcctcgg ccaggacgtt ctggccgaac ttttggttca gaacggccag
    19261 ctcctcgttg accttgcgga gctcggcttt tttcgcctcg tccagggcgg ctccgctgcg
    19321 gacgaaatgc ttgtaggtca aatcaagcaa ccgggcctgc tcggggctga ggccgaatcc
    19381 ctctttcttc tcatagacgg ctttaacccg ctcgaacagc ttcgggtcga gggagatgtc
    19441 gtcggagtgt ttggagagaa ggggggccag ctcggcctcg attttttgaa gctcggggtt
    19501 ggtgtggttg ttggtcaggg cgaaaaagac gtaggcgact ttcttaagca gcgccccgct
    19561 gtgttccagg gcttcgaccg tgttggcgaa ggtcggcggc tcgatcgagg aggtgagggc
    19621 ggcgacctcg tttttctgcc gggccatcgc ttcccggaac gcgggcagga aatgggcctc
    19681 cttgatcagg tcgaacggcg gggtctcaaa cggcgtcgtc cattcaacca ggaaggggtt
    19741 gacctcggcg gcggccgtct gcggcgggcg ggcgaggacg gcggcgacga tcaccgtggc
    19801 aagaatgaga acgaacagga ggatcttttt catcggattt tttctccttt tctcggcatg
    19861 gccgaagggg gcgttcatcg gccgtcgggc ggccggagga tttccaaaaa ctcagagaga
    19921 accatggccg tcgcccccca gatcttgtgg gggccgacgg cgaagaaggg gacgagccgc
    19981 gggccggtct cgaacgtcca tgtttcccgg cggaggttgt gatcgtcgag aagatcggcg
    20041 agcggcgcct cgatgatttc atcgacttcc ccggcttggg gcacgaaggc cggcgggacg
    20101 tccgtttcgg cgacgaacgg atagatgcag tatccgctgg gggggatgta caacggcgtc
    20161 agggccccaa ggaggcggag ggtggagggg acgacgccga tttcctccat cgcttctcga
    20221 acggccgcct cggccggcgt ctcgccggtt tccacgtggc ctcccgggaa agcgatctgc
    20281 cccttgtgat gaacgacgtt ttgcggcctt cggatgaaaa cgagatgggt ttttccgttc
    20341 cggggataaa gaagggcgag gaccgccccg ggaagacagt cgcccccggc ctcgacatag
    20401 gttttctggc cgggcggcgg ggggaggggc atcatccgaa gctgggccgg agggcccgga
    20461 aggggccgtc ggagccggtc cttcagggat ttcaaagcct cgttcatgaa agagtattat
    20521 acatcagatg ggcactccgt ttcctccaaa cggcggaatc cgcccgatgg aggaacgcgg
    20581 gcctaggaac ctaatcgccg tgaaatacgt cttaatcaat gccttctttc ccaaaataga
    20641 caggagattc caaaatgagc ttccttccct tgactgaaaa ggcccgccgg ctcggcgctt
    20701 gtcttgccct tgttcttttc tcggccgcgg ctggatccgt ttcggcccaa agcctcgata
    20761 ttccatcgaa aaaatggggc ctgagcttcg gcaattccaa ggaattcacc ggtttgcggt
    20821 tcaatttccg cgaccgcggg gtgatccgga tcaccggcgt caatgtcacc ctgtggactc
    20881 cccggacgct cggcgaggaa gatgattcca ccgtgacggg actgtccttg ggattggtcc
    20941 ccggggccgg ccgcatgagg ggagtccagc tcggcctgct cggagtcgcc ggcaatcgga
    21001 gcatcgccgg agtctcggcc ggacttttgg gcgtgggggc cggcgaagac atctccggct
    21061 tcaatttcgg cggcctgggg gtgggcgccg gcggcagcgt cgcggggatc aatctcggcg
    21121 ggctcggcgt gggagcgggc gaaaatgtcc tcgggatcaa catcgggggg ttgggcgtcg
    21181 gagccggcaa gaacgtcacc gggatcaaca tcggagggct gggtgtcgga gccggcggcc
    21241 gcctggccgg gatcaatatc ggcggtctgg gcgtcggcgc cggagaacgc ctggccggaa
    21301 tctccatctc cggaatcggg gccggagcgg aatccgtggc cggcctggcg atcgccggaa
    21361 tcggcgtggg cggccgccgg ctgagcggcg tcttcgccgc cggggccgta atcaagctcg
    21421 tcgatgacgg ccgcctgcgg ggcgtcgccg tcagcccctt caaccagctc aaggggacgc
    21481 tgaccggcct ttccatcgga atcgtgaatt acgcccgccg gatcgagaag ggaatccagc
    21541 tcggactcgt caatatcgtc cgggagaaca agcccggcct gcgggtcctg cctctcttca
    21601 acaccgattt ccgctgaggg ccgttccccc ggccgccgga cgggggaagg cgcgaaaccg
    21661 ggagccccta catgttccag cggtaggaca ccttgaccat gaagatgttt tcgccgggcg
    21721 ccgtgagaag gtcggagaaa tcccgccgga ggttgaggat tccggaattg gcataatccg
    21781 cccggttctg ggtccagacc aggtaaatga gcgagccggg gaggtattcc cagcggaaaa
    21841 cgaccgtgcc cctcagggat ttcacgttga agtcgggatt gccgaaggtg aacggggagg
    21901 ccggccccgt cccatcggga tcgatggtat atccgccgcc ggcttcctcg accgttgagc
    21961 tgttttcgcc gtacagattg tattcatact tggccggccg ggccagctcc ttgaaccggc
    22021 tgtaggctcc gacggcgatg aagggctgga ggtaggcctg gagggacagc cgcggagtga
    22081 acgtccagtt gaggcggatc tcggccgaga cgactctctg ttcgagccgg gcgaagacat
    22141 accggtttcc gaacgtgggc gtcatcgagg cgtcggcgaa gcggtcgacc cactgggttt
    22201 cgttcgtctc ggcgagatat gacggcccgg ccgaaaagct gacgttggcc gccggcttcc
    22261 agcggaggga gagcccggcg ttccacgaac ggccgtcccg gcctccgtcg gtgatgcccc
    22321 cgcttccttc gaggacgact ttcttgcgcc cgtccgtgct gagcatcagg aacacgttcc
    22381 ccatgccggg cgtccaggtc agggggcctc cgcgcgtctg ccggttgttc agagacccgg
    22441 ccccggcgaa gagctgggcg gaaaaatacc agaagttctt gaacaatccc tcggcgatga
    22501 ggattcctcc cttgccgacg ttgtgcccgc cgaaatccca attggcgaat ccgccggcgc
    22561 cgacgaggat ctgctggaaa atcttgccgg gctcggtgaa gttgaggccg ggaacgacgc
    22621 tgacgttgat gacgtcggac gttccggctt ggtagcccgc gtcattggga ttgaatccgg
    22681 gcgaaagcat tcccaaggag gaaagccaga tgaaccggcc gttctgcttg gcgaggtgga
    22741 cgcgggtccc ccagcccgaa agcgagacgg ccgccgggtc gacttcgacg tgggccgcat
    22801 ccggcctttg ataatagtgg atcgaggaat tctggattcg gaggatgttc tcggagctcc
    22861 ctccgatccg cgttcccccg atccagccgc cgaacaccca gtctttttta gcgtcgagga
    22921 acgaccatcc gtccacggcc aggctgaagg cgttccggtt gagaaggcct tcgagggccg
    22981 ggttatcgag gtcccggacg acgcccgtgg ccaagagccc gatcccgtgg cggccgctgt
    23041 cgatgtcctt ctggatccgg aagatgccgt aatatgacaa cggctccact tcttctctgg
    23101 aacggaggcc ggacaggtcg atgtcggcgt attcccgcga ggtcagggcg ttgatgaacc
    23161 cgacgttcca gccgccgatc ttgccggtaa gcttggccgc cccgagaatc gtcgagcggt
    23221 cgggaagtga aacatatcct tctccggaag gagatccctg cggctcccgg ccgatccggc
    23281 ggctgtagaa aagcttcgga ttgggccagt tgatattggc gttgaaataa atcccgcccc
    23341 ggccgaagtc gttgaaaatg gacgcgccct cgatgaaaaa cgggcgtttt tcctcgtaat
    23401 aggtttcata agccgtcagg ttgacggagg ccggatcgac ctcgacctgg ccgaaatccg
    23461 gattcaccgt ggcgtcgagc gtcaggttgc tttttaaacc ggccttgaga tcgaagccgg
    23521 cgttgccgga cgcttttcgg ccggtttcga agggatttcc ctcctcggcc gggcggaact
    23581 gggcctggcc ggtggcgtac ggagtgattt ccacgtgaac gccgggggag atgccgcgga
    23641 tgccctccag gcgagcgaat ttggaaacga acgccgtttc gttcttcggg caccaggcga
    23701 aggacgccgt ttcgtttttc ctccggattt tccggcggaa gttgacgccc cagacgtatt
    23761 cctccatacg ggaaaaccgg atctggtgga aggggatcct tatctccacg ttccagccgt
    23821 cgccgtcgat cgcggctttg gattcccaga cgccgtccca agagctgtcg tcggcgatgt
    23881 cgttattaag ggcgaagtcc atgatcgagc cggaagggtt cacgcagaac atgtatcccg
    23941 tccggcggtc gtaataggga tcgacggcga agtaaaacca atccgattcc accatcgaat
    24001 cccggcggcc gaggaggccc gtgattttat ccggttccga atcccggcac ttggcggcca
    24061 cgtacaaggc ctcatcgtcg taggcgaccc agacttcggt cgtctccgtc gacggttgtc
    24121 cgtcctgcgg atcgttctgg gtgaagccgt tcgctccctc gcccttccag gcgctatcgt
    24181 cgaggcggcc gtcgatcttg accggttcct ccagccggaa ggcttggacg atcttctggc
    24241 ccggctccgg agcgggaacc ggcctttgtt tcgccgcggc gaaaacgacc ggccaaaggg
    24301 cggccagcac gaaaatcctt ttgaagtctt tattcatcat ctccggtctc cttgatgatc
    24361 ttcctctccg gaagcccgct gccggcattc gccagcccgc gccggctttt tcctccggcc
    24421 gggtgtcctt caatgtatac gtcgaaaaag cgcgaaaagt tctcttttgc gccggggctc
    24481 ttcagaaacg gagaaaaatg cattatgatg ggatttctta aggaggttaa accatgaaga
    24541 aagccctcct ccttttgacg gcggcgctag tctgcgccgg cctgctcccc ggcgtgtccg
    24601 cggattcccc gccctccacc cgcgagatga acctgctctg ctggcaggag ttcggcgagc
    24661 tcgtcccggc ccggatccgg accgtgctgc tcccgacggg atccctggaa ccgcacggag
    24721 tcgttcccaa cgggaccgac agccttgccc ccgaagccat ggcccgggat atcgccggac
    24781 gggtcgacgc cctgatcgcc ccgacgcttc cctatggaat gacggccgac atgaaggcgt
    24841 atcccggcgc ggtctcgatt tccaaggagg cctacgccgc cttcgcccgg gagatcctgg
    24901 agaacctggc cgacaacggc tttcagaacg tcatcatcct caacggccac gggggaaaca
    24961 cggccgtttt aaacgaagtc tcgtctctcg tgtccaacgc ccgccgggtc cggatcctgc
    25021 tggtgaactg gtggaccctg gccgacgaca tcaccaagga cgtgttcaaa caggacggcg
    25081 gccacgccgg caacaacgag acggcctata tccaggcgat cgtcccgaac cacattcatc
    25141 ccgagcgcta ccggccggag atggccgcgc cgctgccggc tccggccggg gctttcgccg
    25201 cccacccggt gacgacgtcg atccttcttt atcagaaagg ccagggatac ccgacattcg
    25261 atccggccca ggcgaaggat tatttccgcc gggtcaacga ccgggtggcc gagctgatcc
    25321 tggacgccgt caagaaatgg gacgccgccg gcgtttacgg ccgggaccgg gacggcggcc
    25381 gatgagcctc cgcttctatc aggtcgacgc cttcacttcg gagatattcc aaggcaatcc
    25441 ggccggagtc tgcccgctcg aatcctggct tccggacggg ctcatgcaaa acatcgccat
    25501 ggaaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttcg ggacacaagc
    25621 cgaaatcgaa gcgatgcggc cggatttcgg aaagctcggc cgggtcgagg cccgcggggt
    25681 gatcgtcacg gcccgcggga ccgaggttga tttcatctcc cggtttttcg gccctcaggt
    25741 gggcatcgac gaggatcccg tcaccggctc ggcccacacg agcctggccc cctactgggc
    25801 ggagaagctg ggaaagacgg aaatggaggc ggtccagctt tcccggcgcg gcggccggct
    25861 gaaggtgcgc ctgcgcggcg accgggtgga catctccggc cgggcccggg cctacctcga
    25921 gggccggatc gagatggagt gagccgcggc gaaaaggaag gagaatccgt gaacaagaac
    25981 cccgacccac aacggaaacc ggcggactcg aaacccttcc gccgggcgat cgtccgaacc
    26041 cccggtccgg acgcggccgc cggcttgacc acggccggct tgggaaaacc cgacctggcc
    26101 ctgctcctgg cccagcacgc cgcgtacatc gaaacgctgc gcggcctcgg cctcgaggtc
    26161 gaggtcctgc cggcgtcgcc gggctttccg gacgcgtatt tcgtcgagga cgcggccgtc
    26221 gtccttccgg agatcgccgt gctcacccgg ccgggggcgc cctcccggcg cggcgaaacc
    26281 gcctccgtcg aatcagccct ggcccggcac cgccggctgg cccgaatcga ccttccggga
    26341 acgcttgagg gcggcgatgt cctctgggcc ggcgggcgct ttttcatcgg gctctccgtg
    26401 cggaccaatg aggacggggc cctgcagctg gggcggatcc ttgagtcttg cggatattcc
    26461 tggacggccg ttccggtcga gaaagggctg cacttgaagt cgttcgtcaa caccctgggc
    26521 gggcggaccc tggtcgtcgc ctcccgtttc gagggccggg aatttttccg cagcttcgac
    26581 cggatcgttc tcgaccccgg cgacgactac gccgccaaca ccctctgggt caacggcacc
    26641 ctgctcgttc cccggggatt tccccgggcc ctcgagaaat acgccggttt gggacttccc
    26701 ctcatcgagg tcgacacaag cgagatgcgg aagatggacg gcggcctgac ctgtctttcg
    26761 ctccggttct gagaaggtga aaacgccgga ggcccggtcc ggacggcggc gcgccgggac
    26821 ggttttagcc gggatgttcc tggccctcgc tcccccggct ctcgcggcgc ccccgctttt
    26881 cgcttcatcc gaaggccgga tccctctgat cgacctggcc ggcgaggcgg gccgccgaac
    26941 gatcgtcgac cgtgaatccg ggcaatacct cggccatccg acgaccgttc tcctggaaga
    27001 cggccgaacc ctcctggcgg tttatcccaa gggccacggc cgcgggccca tcgtcgtcaa
    27061 gcggagccgc gacggcggcg cgacctggaa cgggcggctg gaggttccgg acaattgggc
    27121 gacgagcctg gaaacgccca ctatccaccg ggtcgtcgag gcctccggga aaaagcggct
    27181 gattgtctgg tcgggcttgt acccggctcg gctggccgtc tccgaggacg acggggagac
    27241 gtggtcgcct ctgcggccgg cgggcgattg gggcggcatc gtcgtcatgg catccgtcgt
    27301 ttctttgaac cggcccggcg gatacttggg gctttttcat gacgacggcc gctttttccg
    27361 ccgtgattcc ggcccgtccg atccggccgt ctttacgatc tacaagacgt tttccaatga
    27421 cggaggcctg acgtggacgg aacccgaggc cgtctggagc agtccggctc tccatctttg
    27481 cgagcccggg gctctccgtt cgcccgacgg ccgggagctg gccgtcctgc tccgggaaaa
    27541 ttcgcgccga aagaattccc acgtgatgtt ttcccgggac gagggagcct cctggtcaac
    27601 cccggttgag ctcccggggt ccctgacggg agaccgccat tgtccgaaat acgctcccga
    27661 cgggcgcctt ttcgtaagct tccgggacat ggcgcccgac agtccgaccc ggggcgactg
    27721 ggttgcctgg atcggcgcgt acgacgacct cagaggcggc cgggagggac aataccgcgt
    27781 tcgcctgatg aagaatcaca aggacgccga ttgcgcctat tcgggcgtgg aggttctgcc
    27841 ggacggcacg attgtcgcca cggcgtacgg ccactggacg gaaaacgaag agccgtatat
    27901 cgtcagcgtc cggctgagac tcgccgagct ggacgcccgg ctcggtcgtt gagccggcgg
    27961 ccgttttccg cggcggccgt cagagcgcgt cgatgatggc gttcagcgtc gggctcggcc
    28021 gcatggccgt ctcgactttc ttccggtccg gccggtaata cccgcccatg tcgacgggct
    28081 tggcctgtgc cccgaggagt tcggccgcga tcgtggcttc gctttccccc agccgccggg
    28141 cgatcgtctc gaaccgggcc cggagctccg gatccttgtt ctgggcggcc aaggcctggg
    28201 cccagtacaa ggccaggtag tagtggctcc cccggttgtc gatctgtccg accttccggg
    28261 cgggggattt gttgttttcc aggaatttgg cgatggcctc gtcgagagtt tcggccagga
    28321 ccttggcttt cttgttttgg aagacgccgg ccaggtgctc aagcgaggcg gcgaacgccg
    28381 agaattcccc gagcgaatcc caccgcaggt agccttcctt ttggaattgc tggacgtgtt
    28441 tgggagcgga gccgccggcc cccgtttcga acaatccgcc tccgcccagc agggggacga
    28501 tggacagcat cttggcgctg gtcccgatct cgaggatcgg gaagaggtcg gtgagatagt
    28561 cccgaaggac gttgcccgtc accgaaatgg tatcaaggcc cttgcggatc cggtccagcg
    28621 aatggcgcat cgcctcgacc gggggaagaa tccggatgtc caggccggtc gtgtcgtgat
    28681 ccttcaaata gcgctcgacc ttagcgatga cttgagcgtc gtgggcccgg ttgcggtcca
    28741 gccagaagac ggccggggcg ccggtcgccc gagcccgctt gaccgccagc ttgacccagt
    28801 cgcggatcgg aatatccttg gcccgggaca tcctccagat gtcgccggcc tcgacctcgt
    28861 gttctaaaag cgtccggccg cagggtgtga cgacccggac ggtcccgtcg aaggggatct
    28921 cgaaggtcgt cggatgagag ccgtattcct ccgctttctg ggccatgagg ccgacgttgg
    28981 ggacgcttcc catggtcgcc ggatcgaacg ccccgaattt cttgcaattt tcaatgatct
    29041 ccttgtagat cgttgagtag caccggtcgg ggatcatcgc catcgtctcg tggagcgttc
    29101 cgtcggggcc ccacatccgg cccgattcgc ggacgaccac cggcatggag gcgtcgacga
    29161 tgatatcatt gggaacgtgg aggttggtga tgccccggtc cgaatcgacc atggccaggg
    29221 ccggccgctc ggcgtagacg gccttgaggt cggcctcgat ttccgctcgc ttcgcctccg
    29281 ggaggacctg gattttcgcg tacacgtcgc ccaggccgtt gttgacgttc acccccagct
    29341 ccttgagcac ggcggcgtgc ttgtccaggg ctttttgata aaaaaccgag acggcgtggc
    29401 cgaacatgat cggatcggac accttcatca tcgtcgcctt cagatggagc gacaggagga
    29461 gaccctgggc cttggcctcc tcgatttttt cggcgtagaa ttttcgaagc gctttccagc
    29521 tcatgaaggt cgccgaaaga atttcatccg ggagggcggt caaatcctcc ttcaggacct
    29581 tgaccgtccc gtccttgccc gtgagttcga tccggatcgg ccgttccttt tggttgaccg
    29641 cggatttttc gtggccgtag aaatctccgg cggtcatgtg gaccacgcag gctttggaat
    29701 ccggcttcca gggacccagc ttgtggggat gcgtcttgga gaagtttttg accgaaagcg
    29761 gcgccctccg gtcggagttg ccctcgcgga ggacgggatt cacggcgctt cccaggactt
    29821 tggcataacg ggcctggatc actcgttccg cttcgttttg cgggcgttcg ggatagtccg
    29881 ggagggcgta gcccttggac tggagctccc ggatggcgcc ctggagctgg ggaatcgagg
    29941 cgctgatgtt cggcagcttg atgatgttgg cctcgggccg ggccgtcaac tcgcccaatt
    30001 gggccaggtg atcttggatc cgctgattgt cccgcaggtc atcggggaaa ttggcgatga
    30061 tgcgtccggc gagcgagatg tcgcgcgttt cgacttcaac gcccgatccc ttggtgaatc
    30121 cctgaatgat cggaagcagg gaataggtgg ccagagccgg cgcttcgtcc gtctccgtcc
    30181 agatgatcgt ctgcttcgcc atgatccctc cttttccgct ttatgttctg ttccgcccat
    30241 gcggaaccgg gatgacacgg ccaaaagaaa tgtacgatag cggaattgcc gggaggtgtc
    30301 aatcggcggc cggtccgatt gacagcccgg ccgcctgaag atatagtgaa gccatgggat
    30361 ccgacggcgg gcggaaggcg gggccggaga cgcgtcctct ttctcatcgt tccctgtctc
    30421 tgtagaaatt ccagcggatt cgttttccgg ccgccgcgga ctgcctgccg gcgtcggacc
    30481 ccggttcaag gagaccggag ttgaagcgga tcgcgaaatt ttttttcgcc tcggccggac
    30541 tcatttgcct ggttcttccc ctttcggctc aggaaaaaac gttggacgag ttgctggctc
    30601 tcgatctcag cgagctgctc gacctgaaag tcgtttccgc cttgaaaagc ccggaaacga
    30661 tcgcccgaac cccggccacc gtccgggtga tcacatccga gcaaatccgg gacaacggct
    30721 gtctgaccct tgaggatgcc ctggccgacc tccccgggtt tcaattccgg aacatcctgg
    30781 gcttcaactc ttattccttc atccggggag tccccggcca gaacaacaag atcctcctgc
    30841 tcgtggacgg tctccagatc aacgagctga actcgggcgg attctacggc ggaggccagt
    30901 tcaacctggc cgaggtggag cgcatcgagg tcatctacgg cccggcttca gctttgtacg
    30961 gaaccaacgc cctgtccggg atcatcaata tcgtcacccg ggatccgcgg gccggctcaa
    31021 ggggccgcgt ttcggccggc ttgggaacct tcggcgcgcg gctggccgac gcccgttacg
    31081 ggtattacga taaaaacgcg gatttcggat tcacggcggc ggcgatgctc aagcggaacg
    31141 agaaagccga tttggccgga gcggccggcg atttcaactg gaccgaggcc atcgagaatt
    31201 tcgaaaacga cgcggccgtc caagcccgtg ttcatttcaa ggattttgag gccgggtttc
    31261 tcctccagga tgaaaacgcc tcgcgggcga cggtggaaaa gacggccgga gaggatcttt
    31321 ccattccctg gagcgaccac ggcgtcaact ggcacatccg gttcatcaat gtctgggccc
    31381 gctattccta cgagaagata aagacttggt ccttccgcaa cacggcgtat taccggaatt
    31441 caaccgtcct ggacgacacc gtttcggaaa tcgagctggc gacttccgat tccccgggcc
    31501 ggcaattccg ttgctaccgg cccaacagcc tgatcggcaa tgaaacccag gttcaatggg
    31561 cgccggatcc gcgctggcgc ttctccgccg gcctcgtcct ggagcgggaa cggctggccg
    31621 agggattttc cgtcaccgtg agctcatcgg cggacgccct cccggcggtt ccgctttccc
    31681 cggccatgat gaccaaccgc ttgttctccg cgtatgccca gatccaaggg accccggcta
    31741 aaggccttga tctttttctc ggcctccggc acgacgactc cagctattac ggcgcggtga
    31801 acaccccgcg gctgggggtg atttacaacc gggggaaact gacggccaag gccttgtaca
    31861 tgcgggcgtt ccgggcgccg aagccgtggg attacgcggc cggcgcgggg aatcccggcc
    31921 tgcggccgga aaaaatcgat tcgtttgaaa tcgccggcgg ctggtccttt tccccgttct
    31981 tgcggctgga cgtgtccgtt taccgcaacc gcttgacgaa tctcctgagt cgggaggacg
    32041 aaacgacgaa cagccgctgg atcaacgccg gagccctgac gacggacggc ggcgaagccg
    32101 ggctcctctt ccgccgcggc tcctggaggg cctgggccta ctacacgtat accgcttccc
    32161 tggacgaaac gggcgaacag gcgccggaaa tcgctgccca ctgcgccacc ttcggcgcgg
    32221 cttatgcctt caccccgcgc ttgaccctca gcctgcgagg ctgttatttc ggggaacgga
    32281 agaatcccaa gatcatcccc tccacgggga gcgaccggat cgaaggagcg ttcgtcgccc
    32341 gggccgccct ctcctggcgt ctgccggcgg gttttgacat ccaactcgtc gtcgataatc
    32401 ttttcgacgc cgtttatttt catccttcca acctgccgcc cagccgcttc cgtcagccgc
    32461 cgcgctcgtt ccgcctcgcc gcggggtatt cgttccagtg atgaagacgg cgcggttccg
    32521 gatcggatgg attctcgcgg ccgccggagc cgtgtttctc tccggcccgc cggccgtccc
    32581 ggccgggaaa accctcgtca cggacacgtc cgcttcggaa gccgcggtca aggcggtttt
    32641 tctttaccat ttcatccgtt acctccaatg gcccggcgaa ttcgaccgcg agccctgccc
    32701 gatcgtcgtg ctgggagaca gcgcgatttg ggagccgctc caggaaatct cggagaagaa
    32761 aaccaacggc tccgttctcc tcctggtccg gcggtgcgag gagttgaaag acgccggccg
    32821 gccccggatt ctcttcctgt ccgattcctc cgcgttcctc ctccccgaag ccctggaaaa
    32881 gttccggggg acgagcgtcc tcgtcgtcgg ggagaccgag ggcctggccc ggcgcggggc
    32941 ggcgatcaac ttcgttctgc gcgacgaaca cgtcaagttt gagatcaacc tcgatgcgtt
    33001 gaagcaagcg ggcatccagg ccggctccca gcttctgcgt ctggccctcc tggtcgaggg
    33061 ggataaggcg gccccggggc gatgaaattc aatgacctgt ccctggcccg gaaaatcatc
    33121 ttcctgcagc tcttcgtcgt cttcatcgtc gttttcctgc tcagcggatt caacgttctg
    33181 tcctccgccc ggttttaccg caactccctc cggacgaagc ttgaatcgat ggccaacctc
    33241 ctcggctaca actgcacgtc ggcccttctc ttccaggacg gcgtggatgc cgcccgaacg
    33301 ctccgctcgc tggaagccga ggcgctcgtg acccacgcct ggatcctgga tgccggcggc
    33361 cggccgttcg ctgcttaccg gaggaaggag acggcggcgg agccgccgcc gcaacgggga
    33421 ggcgactacg aggaaaccca gggccggttc ttcgtctttt cacggcgcat tctccaggac
    33481 gacgagccga tcgggaccgt ccttttccgg tacgacttgt ccgagtaccg gatgatcctt
    33541 ttccggaacg cgctcgggac cggggccgcc ctgctggcca gcatgctcct ggccttgctc
    33601 ttgacggctc gcagccagag gaccctgtcg cggccgatcc ggcgcctggc ggaaaccatc
    33661 cgcctcgtgt cccggacaaa ggacctctcg atccgcgtcc ggggggagga ggcgcggaaa
    33721 gacgagatcg gcgttctcca ccgcggattc aatagcatgc tgtcggagat ccaaagccgg
    33781 gatgcggaac gggagcggat gattgccgcc ctccgggaaa gcgaggagaa ataccggact
    33841 ctggtcgaaa acgccgagga cgcgatcgtc atcatccagg acgagcgctt tgtctatgtc
    33901 aacccgagcc tgggcgccat ggccagaggt caagccgggg aatttctcgg ccggccgttc
    33961 ctcgacgtga tcccggaaga ggagcgggag cggttgacgc gctactaccg ggaccggatg
    34021 gccgggcggg aaaccccgac gatgtacgaa accgcgttga tgaacgccgg cggcggacgg
    34081 atcccggccg aggtcaccgc cgcccggatc tcctaccagg ggcggccggc cgatctcgtc
    34141 atcgtccgcg acatcagcga gcgcaagaaa gccgaggagg aaatccgcaa gctcaacgaa
    34201 ggcctcgagc gccgggtgga ggaacggacc cgggaactgg ccgcggccaa cgagcggctg
    34261 atcgagctgg accggctgaa aagcctgttc ctggccagca tgagccacga gctgcggacg
    34321 ccgctcaact ccatcctcgg attcaccggc ttgatcctca tgggaatgtc gggcgacgtg
    34381 tcgacggagc agagaaaaca gttgaccatg gtccagagca gcgccaccca cctcctggcg
    34441 ctgatcaacg acatcctgga tatctccaaa atcgaatcgg ggcggatgga tctgacgatc
    34501 gagtcgttcc cggcggccga cgtcgttcgg gaaaccgtga agagcgtcac cccccaagcc
    34561 gaggccaagg gtcttcgggt gagggtcgat gcgccggacg gcttgtttct ccaaagtgac
    34621 gtccgccggt tcaaacaggt gctgatgaac ctgctcggca acgcgatcaa gttcagcctc
    34681 cggggcgaca tccgcgttca agcggacgtc cgggaggaat atctcgaggt ccgtgtcgcc
    34741 gaccaaggga tcggaatccg ggcggaggat ctcccgaaac tgtttgtccc cttccagcag
    34801 atcgacatga cgatgaccaa ggcgtacgag gggacgggct tgggactcta cctatgcaag
    34861 aagatcgtga ccgcgctccg gggggggatg acggtctcca gcgagcccgg cagcggcagc
    34921 gtctttgtct tccgcctgcc tctccgatgg aaggaggatc cgcgatgaag acggttctca
    34981 tcgtcgagga taatgagaat aacctgtaca tgatgcgctt tatcatcgac aagcttggtc
    35041 acgcggtcat cgaagcccgg gacggggcgg ccggcgtggc cctggccaag gagcggcggc
    35101 ccgacctgat cctgatggat atccagctgc cggtgatgga cggctacgcg gccacccgga
    35161 aaatccgggc ggacgaggcc ctggccggca tcccgatcat cgccgtgacg tcctatgcca
    35221 tggtcgggga ccgggaaaaa gcgctggccg cgggatgcac ggcctacgtc gaaaaaccga
    35281 tccatccggc cgcgttcatc ggcgttctgg aaaaatattt ataaatcttc ggagagatga
    35341 tgaagatcct gattgtcgac gatgaagaga aatcccggta tttgctcgcc cgccttttct
    35401 cgggaagcgg acacgccgtg gaggaagccg ccgacgggaa aatcgcgctg gagatgctcg
    35461 aaaaagaagc gtacgacctg gtcatctcgg atatcctgat gccggtgatg gacggctacg
    35521 cgctttgtcg cgagctccgg tccagggagg gcctgaggga aacgccgttc gtttttctga
    35581 cggccacgta tgtcgacgag aaggacgagc ggttcgccct ccagctgggc gccgaccgtt
    35641 tttatcgcaa gcccgttgat ccgcggacat tcgtccggga gatcgacctc ctcctggagg
    35701 agatccggga tggaaaacag cgccggttgg ggccggagac cgatgaaaag gagattctca
    35761 ccctctacaa cgagcgcctg atcaacaagc tcgaaaaaaa aatcgtcgcc ttggaaaatg
    35821 aaatcgccga gcgcaagcgg gccgaggaca aggcccgggc ggcgatcgag gaaaacgagg
    35881 ttctcctccg cgaggtccac catcgggtca agaacaacat gcagctcgtc tccagcctga
    35941 tcaatctctc ggcccggggc cttaaggatc cggcggcgct cgatgtcctc cggcagatcc
    36001 ggctccgcat ccgctcgatc tcgatgatcc acgagaagct gatgaagtcc ggcgacctcg
    36061 tccgggtcga tttcgccgat tttctcgacg acatggccat tcactttttc cagttctaca
    36121 aggccgatcc ggaccggatc agcctgaagc tggaggtcgt gccggtgatg ttgccggtcg
    36181 gcctggccgt tccctgcgcc ctgatcgccg gggagctgat gtccaacgcc ctcaagcacg
    36241 ccttccccgg cgggcggcgg ggaaaaatcc tcgtcggctt gaagcctctg gagagcggcg
    36301 agttccttct ccgggtcagc gacgacggcg tggggtttcc ggaaacggcc gatatccgga
    36361 aaatggaaac gctggggatg acgattctca acgccctggt agcccaaatc ggcggccggc
    36421 tcgagatcct ccgcgacggc ggctcggact tccggatcac tttcgcccgg cggccgttct
    36481 gaccgccgga acgccggacc ggtctttcgc cgggcggcgc ctcagccctt ctttccgccg
    36541 gcttcccagc ccgcggcgac ggcgtccaga tcttcgacga aaacgctccg gccgtcgaaa
    36601 cgcccgcccc gggcgatgac gagaccggga gcccgctgga agagcgttcc ggcgaaccgg
    36661 ccggtttcgg tgatttcgat ctcctcggtc acggccatct cgccctcgaa gtcgcccgaa
    36721 acgacggctt tgcgcgcgcg gacgtcggcc cggaccttgc cgccggcggc gatgatcagc
    36781 tcgttctcgg cgatgatgcg gccgtggaat gagccgcaca agtgaatgac ggccgtcgac
    36841 tgcagaacgc cgtccatcga ggcgccctcg ctgatgacgg ccaggctttt ctcgaccttg
    36901 tcttctctgg atttttcttt ggacacggca tcttctccgg ccggaatctt atgccgggac
    36961 ggccccgaag tcaatccggc cgatcgaccg ggcagagccg gccgcggccg gggggggccg
    37021 cggatctccg ccgaatgatc atccgacgac gggagacctt cggcgacgaa tttgtttgac
    37081 agccgcggac cgaaaaaggt aaattccatc cgaaaacgat gttccaggcg acttcggtcg
    37141 agcacagaat tgctcccggg gcacggcgac cgggggcttc ctttccgttc gcggtgtttt
    37201 ttttcctgct gattttcctg gccctcgttc catccggacc ggccgccgct ccgccgggcg
    37261 cggatccggc gatggccttc ggattcaagg gctacggcct cgaccaaggt ctcacgtctc
    37321 cctcgatcac gaccatggtc caggacctgg acgggttcct gtgggtgggg acggaggacg
    37381 gtctcttccg gctcgaaggg aagcgtttcc gccgcttcgg aaccgaggac ggtcttcccg
    37441 ccaataatat cgaagccttg tccctcggcc gtccccgggg catttgggcg accacttcca
    37501 aagggatcgt ccggtgggac ggccggcgat tcctgcgtcc gggaggtttc ggtttcccgg
    37561 gcttcgacaa ccggccgggt ctcgccgtcc ccggcggagc gatcattctg tctcatttcg
    37621 aggctaagca gcgcttcttc agctccacgg atgaaaagag cttcagcgag ctgaagggct
    37681 tgccctgggg cggcggggcg aattgcgcct ttttcgatgc cggccgcaat ttcctgctgg
    37741 tgtccctcca ggaagggctt tgggcctggg atggaaaggc ttggaaaagc cgcgtccttt
    37801 tcgacgacgc cgaaggccac aaggatattc gaatgcttct cgtcgaccgg gccggccgca
    37861 tctggatccg cctgacggaa cggctttacc gcctggaatc cttcgaggcg cccccggtcg
    37921 cggtgacggt tccggaaccc ctgtctatgg tcaatcattt tttcctggtc gaggacgcgt
    37981 tcggccgcgt ctggaccaat accgcccgga atctgatctg ggtttcggaa tccggatcgg
    38041 gagtgctggg cgagcgccag ggccttcctc ccaccggagc gttcgtcctc tatgtggacg
    38101 tccaaggaac gctctgggcg ggtggagacg gcgttttcaa gcttctggga gattctcttt
    38161 ggagctgggc cacgcggaaa gaaggccttc ccgcggatgt cgtttggtcg atctgccgaa
    38221 cccgggacgg acgcgtctgg gcgggaacgg ggggcggcct cgctttcgga acccccgaag
    38281 gctggcgtct tgtccctgga accggccggt gtcagattct ctctctctac gaggacgccg
    38341 ggggaacgct ctgggtggga cacgagccgg gaggcgggcg aaccaccgga ctgatgagcg
    38401 ttccgccggg cggaaccgaa gcctggaccg ttcccgtctt tgggattgag acgcgagggc
    38461 cgttttttac gatggcctcc gattccaaga attcgttctg gatctcggcc gccgaggccg
    38521 gtctgttgcg ggcctttccc ggtcccgccg gcgtccgcgt cgagaagttc gccgttcccg
    38581 gctggattga ggagccgttc atcactaaga tcgaccccga cggagcggga ggcatctggg
    38641 tttccagcga ccagggtgcc gctcattgga acggccgcga ttgggcggtc ctccccaagg
    38701 gtaccttgtc cgaccaggaa atgatgtcgg ttttggccct cggctccgaa agcgcctggt
    38761 tcgccctctc cagcggcaag cggcttgtcc gcgtgaggag ggaaggggac gctctccgct
    38821 tggccgagct gctgccggcg tctcaccctc tttctcagtc gctgatcttc gccctggccg
    38881 ccgatcctta cggcgtgatc tgggccgcga ccgcccgtgg attgatgcgc tgggaaggag
    38941 gccgggtcga acgatatggg cggaacgcgg gcttccccgg cgaggactgc gcccaaaacg
    39001 ccctctgggt ggagccgaac ggcgacgttt gggccgggtt gagcgtgggc ttggttcatg
    39061 gagccatgaa tctccgccgt gagccgcaga cgcctccggg cgtcgtcctc cttgaaagca
    39121 aggacggtca gggccgcctc ttggacgacg ccgatcccgg ccccacggtc tcctggccgg
    39181 cccgaacgat ggctttccgt tatgctctcc gcggctcgcg atggacggag ggagccgggt
    39241 ttcaagtccg gcttgtcgga atggaggagg cctggcggaa cacggatgtg tccgaggccc
    39301 tgtttcccgg gctggatgcc ggccactacc ggttcgaagt ccgggcggtg tcgacgacgg
    39361 ccgagaccgg cgcgccggaa agcttttctt ttcggatcct ggcgccctgg tggcggcgct
    39421 ggtggtccca attgggcgga gccctgatcc tggcgggagc ggttgttctg gcgttccgcc
    39481 ggcgcacggc gatccttctc cgccggaacg aattcctgga atcgctcgtc caagcccgaa
    39541 cgagcgagct ggagaaagcg aatgaagccc ttcgggaggc cgtcctgatc gatcccctga
    39601 cggggctcta caaccgccgc tacttgacct tgaccatgcc cgaggaggaa atccggctgc
    39661 gccggatgtt ccgaagctat ctccagaaag gcgagagccc cttgaaccgc aacgaggacc
    39721 tggtgctctt cctgggagat ttggacttct tcaagcggat caacgacgaa ttcggtcacg
    39781 ccgcaggcga ccaggtgatc atggaaaccg cccgggtgct tcgaaccgcc agccggacgg
    39841 cggacaccct ggtccgctgg ggcggagagg aatttctcct ggtggccaag cgtacggatc
    39901 gaacgaaggc ccacttgatc gccgagaagc tttgccgggc cgtccgcgac catgtcagcg
    39961 tcctgcccgg cggcgcaacg accggctgca aaatcaccat cggcttcgcg gctttcccca
    40021 tcctcgaaca gaatcccgag gccttcactt gggaagacgc cctgcaggtg gccgaccaat
    40081 gcctttatgc ggtcaagcgg gccggcgggg acgggtgggc cggcatccat acgccgggac
    40141 cgctggattc cgttgaaatc ttgtcgcgcc tgaggatgga cttggccggc ctcgtccggg
    40201 agggcttcat ccgcgtccaa agcagcttcc cggaaggctc gattttcaac gggctcggcg
    40261 ctccgccgaa aaaatactga cggaagcgga aaaacagaaa agaatcgccg gcgcgcggtc
    40321 tgatcccccc ggaggcctta tattagaagg ggaggagaat aatgagcgcg cgcgctgttt
    40381 tcctgggttt ggccctgatc gccgccctcg ctaatgtcgc cgccttgatc atgatcatgg
    40441 cggctctcga ccggcggggg cggaagacga acatgtttat ggctcgtctt ttcacgggca
    40501 ggtacctttc ggcgtacaag gagacgacaa aaaaggaaat cggaaagccg ggtccgctct
    40561 attccctttg gctcgtcgcc atcatcctgg ccctcgtctt tgcggtcatc gccgtctttt
    40621 tgcctcagga ataatcctgg gttggccgat gaacaagcga cggagttacg ggaacggccg
    40681 cggccgaggt caggagccgg cggccagggc ggcgatctcc cgggcggccc ggcgggcgac
    40741 ggtttccagg tagtgcgcgc cgcgccggat cgcgtcctcg agcgagaggg cttcggggcg
    40801 gatggcgtaa gcggcggcga acaacccggg gacataatcc gataacggcc cgtcgatcga
    40861 gccgcaaaaa gcgacgcagg gtttcttcag ctcccgggcc agccgggcga tgccgtcggc
    40921 cgtctttccc cgtctcgatt ggaaatccaa ccgcccttcc cccgtgacga cgagatcggc
    40981 ccggcgggct ttctccttaa atccggtcgc ctcgatgatc gtctcgactc cccggcggat
    41041 caccgctccg aggaacgcgg ccgtcccgaa accgaggcct ccggccgcgc ccgctccggg
    41101 aagatcgcgg tattttcttc ccagggccgc ctcgacggcg tcggcgaacc gccggccggt
    41161 ttcctccagc cggccgacgg tctcagcgtc cgctcctttc tgaggggcga agaccgaggc
    41221 cgccccgtgc ggcccgagaa gaggattgtc gacgtcggcc aggacttcga aacggacggc
    41281 ctggagacgg ggattgagcc cggcggtctc gaccgtccgc agttcggaga gcgccgccgg
    41341 ccggggcggg agaatgcgtc cctcgccgtc gagaaaccgc gctcccaggg cggccgccat
    41401 ccctgccccc cagtcgttcg tcgccgagcc gccgaggccg atgaggacgt tctccggccg
    41461 gtgctccaag gcggcctcga tctggcggcc gaggccggag gtatcggacc gcaggatgtc
    41521 ccgcctcccc tcgaccagcg tcaggccgct ggaacgggcc gtttcgatca cggcggtttt
    41581 gcccccgggc cgcagaagaa attcggccga gacgggatcg cccagcggac cggggcaggt
    41641 cacggaaatt ttccggccgc cgccggcgcc gtaaaacgcc tcgacggtgc cgtcgccgcc
    41701 gtcggccgtg gggacgacat cgatcgcggc cgggacgccg aggcgggcga atccctctcg
    41761 gagacccgcg gccacggccg ctccggcttc gattgaagtc agggaaccct tgaaaccgtc
    41821 ggtcgcgatc agaatgcgca ttttattctt cttcactccc cggacggaat ggattcggga
    41881 acgcagtagg ggccttcggg gaagacggcc agtgttttgc cggggccgag ggcagtgtcc
    41941 aaggcggatt gaacggccgc gcccagccgg ccgcgggaag ccgtccggcc cgtcaacgtc
    42001 aagatcctca tcgtctcctc cggcagtccg tcggagagga aaagcaagcc gccggccccg
    42061 acccgggcca gggcccgggc ctgcatctgc agctcccatt ggtccacggt gaagacgccg
    42121 gggcgcttga tgtcggccag gaaatcgtcg aagcggccgg cgtactccct caacaacccg
    42181 gcgtaagcgg ggcttcccag cccctcggag cagccgccga aggcgatgac cgtcccccct
    42241 tcccggacgg ccggaaggca ggagacgaaa cccttgacgc actggtaaaa cgtcgcgtcg
    42301 aggggatagc cgccgcttcc cgtcaagacg acgtcggctg gacggcggac cggacgcacg
    42361 gcgcaggttt ggacgaaggc gcaggcggct tcgtgggccg gttccagggc gccggcaaac
    42421 gcccggacaa ggcggcggcc gcggtccacg acgacgttga gagtgaaatc gacgccggcg
    42481 gcccgggcga ccgaaagcga ctcctcatgg aggggatttc cttcgagaac ggcgttcgcc
    42541 gcccgcggat cggaaagcat cttttccccg tggaattcgc ggatcgtttc gagcgaggac
    42601 aggcccgggc agacgctttt ccggccgccg gagaaaccgg ccatgaaatg cggctcgaca
    42661 agccccgtca ccagccggaa gccggcttcg acgaaccgcc ggttgagacg gatggccgcg
    42721 ccggaggcgc tccgcccggg cgccgcggcc aggccggggc cgtcgccggc ttggtgatcc
    42781 tcgatccggc agcggtcgac gatgtccggg ccgaacatct cccggcgctc ggcggtcgtg
    42841 ctcgggcgat gcatcccggc ggcgatcagg atgagaatat cattttgggg aactccggcc
    42901 gcggcgattt ccccgagaag gggcgccagg aaatcccggt aggggacggg ccgggtgaca
    42961 tcggaaacga cgacgacgac ctcgccgggc cggcgggagc ccagcgcctc tcgaaggggc
    43021 ggggcgccga gggggcgttg gacggcatcg agaacgaggc tcgccgccgg cgccgccggg
    43081 acgggatagc gggaaacgaa gaggtcggcg tcgtcgggaa cacgaaggag gatttcctcc
    43141 cggctggaca aggcccgcac gttcatcgga gtgtcttttt tacggagaaa gctcccggag
    43201 gccggcctcg agcgcctcga tttgggaacg gcattcggcc agcttccgcc tttccttttc
    43261 gacgacctcg gctggagccc gggcgacgaa gccttcgttg ccgagcttag cctcgatttt
    43321 tccggcctcc tcgatgagct tgtcccgctt ggccgtcagc tgccggcgtt cccggaccgg
    43381 atcgaccagg ccgggaacga aaatctccat gcccccggcg acctggacgg ccgccgccgc
    43441 cggccggacg acagccggac ccgcgtccag ggactcgaga tgagccaaat gaatcacgaa
    43501 ggaacgaagg cgggccagga ccgcggcgtc cttttccgtc gtccggacat gaaccggaag
    43561 cttcagggcg ggtgaaacgc cgcggcgggc gcgcatgtcc cggatcgccc ggacgagatc
    43621 gaacgccagg ccgaaatccg cctcgagcgc gtcgtcctcc cattgcgcgt tcgggacggg
    43681 ccagcgggct tggagaagaa gcggggatgg cgggagcggg gccgcgagtc ctcggaccgg
    43741 gcactgcccg ttcagccgtt cccagagggc ctcggtgatg aatgggacaa agggatggaa
    43801 caggcggagg acatggtcga aggccagggc caggacggac cgggccgtcg gggccgcgcc
    43861 ctcgtccctc atccggggct tgatgatttc caggtaccaa tcgcagagct cgccccagaa
    43921 gaattcccgg gccgccccga gggcggccga gggattgtaa gcctggagct cttcatccac
    43981 attccggatg accttggaaa gacgggaaag gatccagcgg tcctctgcgg ccaagtcgga
    44041 ttcggaaagc ggccggaagg gatgcccgcc gagattctga aacatgaacc gggcggcgtt
    44101 ccacagcttc gtgcaaaagg cccggccggt ctcgaagcgc tcgctgatga tcttggccgc
    44161 cggcaggtcg ggcatggtcc cgagaacgtc gaactctttg ccggtcctgg ggtcgatgta
    44221 cgtgaagatc gagcggccgt gtttggccgc ggccagatcg accagctcgc cggtgaaggg
    44281 cgagacggct tggaccggga ggcggatgtc ctgggttccc gtctgcatgt cgcagaggac
    44341 gtagcgcatc gcgtccgtcc cgtacttctc gatgatgtcc tcgggatcga ttccgttgcc
    44401 cttgctcttg gacatccgct cgcccttgcc gtcctgaatg gtggcgtgga taaagctggc
    44461 ggtgaaggga acgtcgcccc gcaggtaaag ccccatgatc atcatccggg cgacccagag
    44521 ggtgatgatg tcgcggctgg tgacgaggca ggatccggga tagtaataat ccaggcagtc
    44581 atcgaggccg ggctccggcg gcgtcagggg ccgctggccg ggatcgagga cggccgtggc
    44641 cggatcaggc cagccgaggg tcgagagcgg ccaaagcgcc gagctgaacc atgtgtccag
    44701 gacgtcggga tcctgaacca agccggcggc ctcgagcttc ggggcgtgca gagtttcggc
    44761 gtcctcgtta cggagcgaga cgaggatcga gtaattccgg gcgggatcga gggcggcgcg
    44821 cccgccgggc tcgaacgaat cgccggcgtc gtcggcgatc cggacgcgca tttcctcggc
    44881 cccggcctgg ggaagacggg ccgcgacctc ggcgattttc ccgccgggga acgttccgtg
    44941 ccagatcggg atccggtggc cccaccagag ctggcggctg atgcaccagt cgcgcttctc
    45001 gccgagccag gcgcggtagg ttccgccgta gcgatcgtcg tcgggggcga acgtcaggcg
    45061 gcggccggtc ggagacttgt agccgggccg ggaggcgtcg atggccgcct gagccagtcc
    45121 ggaggcccgg aactcgttgg gcgtgcccgc cccgcagacg atgccgccgt ccacgtcgcc
    45181 catccggacg aaccattgct tgctgagata cggctcgatc ggcgtcttgg accggtcgga
    45241 atggccgact tccatctccc ggtcctcgac ggattcgagc aggttccggg ctttcaggtc
    45301 ggcgacgacc cgtcggcggg cgtcgaaacg gtcgactccg gcgtagggcc cggccgaggc
    45361 gttgagcgta ccgtcggggt tgaggatgtt gatgatcccg atttcgtcct tgtgccgctc
    45421 ccagacgccg tagtcgttgg gatcgtgggc cggcgtgatc ttgacgcagc cggtcccgag
    45481 ggccggatcg gcccaggcgt cgacgatgag agggaggggg cggtcgagaa gaggcaggcg
    45541 gaggcggcgc ccgtcgcggg ccatgtcccg aaggcgcaac agcaggggga ggacgccgcg
    45601 gcggcgcgat tcgagattct cgagggtttg ttcaagctcg gcccggtctt ttttcggggc
    45661 ggccgcgatt ttaaccgcgg tttcggcgat tcggcgctcg agctcggcgg ccggatcggg
    45721 atggacggcg acggccgtgt cgccgagcat tgtctcaggg cgggtcgtgg ccacgacgac
    45781 gaattccggt tcgccggccc gcggctcgat gaccgggtag cggatgtgcc agaaatggcc
    45841 ttggactttt tcatggacga tttcgtcgtc cgagacggcc gtctggagct ggcagtccca
    45901 gttgaccagg cggtcgcccc tatagatgag cccgtcggcg aagagccgaa agaaagcttc
    45961 ccggacggcc cgggcgcaga cggcgtccat ggtgaagcgc tgccgcttcc agtcgcagga
    46021 gcagcccatc cgctgctgct ggcggatgat ccgggcctgg tattcgtcct tccaagccca
    46081 gatccgggcc accagggcgt cgcggccgag gtcatggcgg gtctttccct cgagctcgaa
    46141 gaggcgcttt tccacgacag cctgggtcgc gatgccggca tggtccgtcc ccggcatcca
    46201 gagggtgttg tcgccggcca tccggcgcca gcggatgagc aggtcctgca tgacgttgtc
    46261 catggcgtgg cccatgtgca gggccccggt gacgttgggc aggggcatca tgatgacgta
    46321 ccggcggtcg cggcggtcgg ggacggcggc gaaggcgtcg gccgcaagcc agcgctccgt
    46381 cacccgccgc tcgtgcgggg acggttcata caatttcggg atttcatccg ccatggtctt
    46441 gtccttcgga tcgatcgagg ccctattttt accatagacg gggcgcttcc tcaaatttcc
    46501 ccttcttccg ttttgaaacc cccttcgcgg attgatataa catatccatg ctgaaaaggg
    46561 cgcggattgt tcctttgaat gaagcagagg aactggatat ccggtattgg aaagaagccg
    46621 ttccggaaga aaagcgggat acgcttcaag ccctccgtga aatgttcttt ttcataaaaa
    46681 atgagaatcg aagcggactt cagagaattt ttcgtattat taaacaagca taacgtcctc
    46741 tacttgattg taggggggta tgcgtattct ttttatgcgg agccgcgttt tacgaaagat
    46801 ttgaatattt tttctcgaca tcatttccaa ggccgatttg atacgaaaca agacggccgt
    46861 gggacggata caggatctca gcgatatcga caagctccag aaaatctgaa agatccgcct
    46921 ttgatcctcc gtttttttta agagttccgg gcggaaatcc tcctccccct tggccggagc
    46981 gggagggagc cggactccga acgcggaccg gaaacgctcc cgaggccggg tttcctccga
    47041 gggcctttta tccggaagga accgtcctcc cggccgccga ttctccgacg ggggatctca
    47101 ccacctcttg tatttccaga aggccaggaa gccggtcatg gaaatcgcgg ccaggcccat
    47161 gatgatccag aaggccggca gctccttaag tccgaacggc agggtcacgt tcatggaaaa
    47221 aatcgagacg accagcgtcg gcaccatgat cgagatggtg atgatgttca aggtcttcat
    47281 caggacgt
//
LOCUS       DAOOXH010000009        46758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_637_length_46758_cov_8.010792, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000009 DAOOXH010000000
VERSION     DAOOXH010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 46758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46758
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_637_length_46758_cov_8.010792"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PKV64_02310"
     CDS             <1..441
                     /locus_tag="PKV64_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009091350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HPN15946.1"
                     /translation="SIMPGKVNPVIPEVVNQIAFKVIGNDLTVTLGAEAGQLQLNVFE
                     PVIAQSIFESIEMLKNGMDTLRLRCVDGITADEAQCRKSVERSFALVTALVPELGYEA
                     CSALAEEAMATGRSVYAVVSEKGLLPKERLDEIAKPENMVRPRT"
     gene            556..1845
                     /locus_tag="PKV64_02315"
     CDS             556..1845
                     /locus_tag="PKV64_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HPN15947.1"
                     /translation="MDIFDKCKTDGGYFGMFRARNDFFFTRPILDPLPGREMTYQGKK
                     VVQWSINNYLGLAENEELKAIAVESARKYGPGAPMGARMMTGNTQKHLDLEAKFAKFM
                     GKEDAILFNYGYLGVLGTIQALCEKNDTILIDKLAHASMIDAMFMSQAQFRVWKHSDL
                     DSLELHLKRVNRDRKGGILVVTEGVFGMTGDIAPLSDICYLKDKYDARLFVDDAHGFG
                     VMGPGGRGTGELQKCLDKIDINLGTFAKAFAAIGGVAGSTHEVCEWVRFNARTQVFAK
                     SLPMIYVDVLSRALDFVADGEARRARMWEVNAKLKAGLRELGYTVGEVPSPVTPVLVH
                     YKDLHIAMAVLQKMREEGIFLTGVIYPVVPRGIVLFRMIPTASHTDEDIARTLAAYKK
                     VRDELHLDLGAVKDALAQYEIEKKSADKDEDENKTEI"
     gene            1842..2636
                     /locus_tag="PKV64_02320"
     CDS             1842..2636
                     /locus_tag="PKV64_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPN15948.1"
                     /translation="MIQGPVLITGASKGIGRALAAALAARGIPVIGTSRRPETIADPV
                     PGVRYLTLDQEDDASVEACAAAAGPVEALVNNAGQSQIGAVEDVSMETVEELFRMDFF
                     GLIRLTKAVLPGMRERGRGTIINIGSLTGTFPPPYQSAYAAIKLGLEAFTKVLRQEVG
                     RQGIKVSLVVPGYIKTHIEPRTIAPAESVYATDLAAFRLARDKKMGKASPPEAAAAKI
                     IKVMEMKNPGPVYYTGRLVPAMGFLKRLLPEKTAQKMIRGFYHLDG"
     gene            3020..3742
                     /locus_tag="PKV64_02325"
     CDS             3020..3742
                     /locus_tag="PKV64_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPN15949.1"
                     /translation="MGKEMMTRAELAEKTGIEPVEIDLWTKAKILRPVGFAEDKSPFY
                     SGPDIGRVAHVRKLRELGYGLEEIQKIIKKYGWPGSGDDQKKDAGKGKLLTVGLLAEK
                     AGVSPRTIKHWEDVGILDPDLRSEGGFRLYDESYVYLCQLVRDLQLFGYSLEEIKAVS
                     DAVRDFLSLQKDLSVYSREEAGRKLDGLQAAAEALFKKMRPLREGIDRWEDLLKKNKK
                     EIAALKAKNAKRPPAASEKTHA"
     gene            3735..5165
                     /locus_tag="PKV64_02330"
     CDS             3735..5165
                     /locus_tag="PKV64_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPN15950.1"
                     /translation="MPKIALIEPRSPNLHIFSKFPSPRLGTVLLGTIMKQRGWDVELF
                     VEDFRALDFGVIGSADIVGISTITSTAPRAYAIAGRVREMGIPVLFGGPHVTFLPGES
                     LEHGDYVLRGEAEETLPAFLEAWRTEADLSEIPGLSFRRGGEAVHNPRSAPPRNLDAF
                     PYADFDLLKPDRRWRGKKMVLPVQTSRGCPFDCTFCSVTGMFGKAYRFRSTEHILGEL
                     RLHADRNRNLFFYDDNFAAHKPRTRELLEAMIREGFKFRWTTQVRADVARDPDLVRLM
                     KKAGGHTVCIGFESVNPRSLQESHKAQSVAELDRAVRVFRRNRIHIHGMFVFGFDQDD
                     RGTFRETVKWARKSRLTSSQFLILTPLPGSAFYKKVMDEERIAFKDWSLYDAHHAVFR
                     PRRLSLAELQKAQIAGHRRFYSLKEQVRNVLEGRWLAIAINQYARALNRTWQKKNRTF
                     LKALDLLRPRRGSRVWVDYRQDVGLD"
     gene            complement(5190..7187)
                     /gene="feoB"
                     /locus_tag="PKV64_02335"
     CDS             complement(5190..7187)
                     /gene="feoB"
                     /locus_tag="PKV64_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPN15951.1"
                     /translation="MSFSGPAEIKVALAGPPNVGKSTVFNLLTGLHQHVGNWAGKTVE
                     RKEGTFPYGRTSFRVIDLPGTYSLTAASPEELIAREFIIRERPDLVVAVLNAALLEHG
                     LYLVAELLSLRVPVVIGLNMMDVARKEGLAIEPGVLSAALGLPVVPMEASSRGGIKEL
                     VKAIAAAAEGGRIPEPQRPVIREDHRLVLDRTGRLLEGTDLEPYPRDWVALKILEGDE
                     DISRKVAAAVPPGRWEEILALLKRHDDALVAVASGRYAWIERMTRAALVRPQTGPISL
                     THRLDRWLTHPFWGLLAFAAVMAAVFSLTYALGIPLQGFLEKSVIQGLQGLVGGWTAS
                     SPWWIRGALVEGILGGVGTLLTFLPILILFFGFTALLEDVGYLARSAFVMDRFMHAMR
                     LHGKSCLPLIISFGCNVPGVLGTRIIESTRARLLTMFLIPLIPCTGRMAVIAFLAPLF
                     FREQAALVAIGLILLALGLLFATGMIGGRLFFRQDAAPFIMELPLYHVPHPRTICDLV
                     RRRSIEFVRKAVVVILPVSLAVWALSRFPEGRVESSYLAGFSRALEPAGTLMGFDWRL
                     MVALFAGFIAKENSLAALAVLFGAGEGVALGDLIVSAYPPAVGLAFMASQLLFIPCAA
                     TVAAFKQESRSWRLTLFNVAYLFILSAAVGIAVYQASRRIL"
     gene            complement(7184..7441)
                     /locus_tag="PKV64_02340"
     CDS             complement(7184..7441)
                     /locus_tag="PKV64_02340"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPN15952.1"
                     /translation="MTPRAAGPMTLAQLSSGRRAVVAGLDGGRRLLARMVSLGFVPGV
                     EVVMVQNPGRGALIVQIREARVALGRGEAHRILIAPARLPA"
     gene            complement(7438..7875)
                     /locus_tag="PKV64_02345"
     CDS             complement(7438..7875)
                     /locus_tag="PKV64_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPN15953.1"
                     /translation="MNSIPAIKSALKASGRRITSKREVILDLLRKIPGHPDAETLFLE
                     AKKIDPRIGLSTVYRTLALLKKEGFIEEHRLGQSHGHFEASRRAAHVHFSCLACGQVI
                     ECENPLVNRLKTEIKRTLNADLKEFHVYAGGFCRSCRPTGGRP"
     gene            8386..8769
                     /locus_tag="PKV64_02350"
     CDS             8386..8769
                     /locus_tag="PKV64_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HPN15954.1"
                     /translation="MTNDRSLSRRERQIMDVIYELKEATVLQVLERLPDPPGYSAVRA
                     LIRVLERKGHLTHRRDGPRYVYSPLVAKEKARRNALRHVIRTFFDGSTESVMAALLDI
                     SEKNLSEDDYVRLRELIDKARREGR"
     gene            8771..11089
                     /locus_tag="PKV64_02355"
     CDS             8771..11089
                     /locus_tag="PKV64_02355"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HPN15955.1"
                     /translation="MQGITATAALLVEGFLKTTLVLVPALLGAAACRRRPASFRHFLL
                     SFALIGLLFLPLFRLLPGGWKTPLLPARPAAAVSSSAVFSSPPPAALSGASAAVSFLS
                     VRDGSPSSRSGESAAGPSNAPTGGRAVPAAVSGAAGGFFPAHAAVFAWFAGFLFLLLK
                     LAFGLVGAYRLTREGWEPGDRGWRILIARFLAVVPLRRGVRLKSHPEVAVPLTWGWRK
                     PVVLIPVGADAWEEAERSAALFHELSHIKRADFLFLLLVRLSLAVFWWNPLCWLAYAR
                     LRKEQEIACDELVLRAGIKPSIYAAGLLAFRSSADFRGRAAAAGPGLLGGPFFKDRLA
                     AILRQKQTFKEVKMKSRIMLAALVVLAIAFIGTARPAVAGNAASSSGAPVDETLSAAA
                     PAAAQEVKAEKETIKEKEKEKTALKEKEKKKEKEKTSVKTIMVTAGGDKDQPIEITVT
                     GEGQIKKLLLDKPLTLKTGEDGRITFSVEGKEVTVPKGGTISIRGAGGALRFIKEGRI
                     VQLEEGEAADLAGDGDKIVVIEKPGEGGSKHTIVKEIRIKKGEAGRAIEIGKEIKAGE
                     QPVKVIIEEHGKGKPGVVWTTAGEGPAALAVIGERDLLEKVKAIREKAEAVKAKTLEF
                     EELEKSLKNLEAELKDAEDRLKNITVRIDQGEDVRIVREDEAGKKSFVWIEAGDAGKD
                     GEPMKVVCRRDAGTLEITLTGMKGPEGRSVHDRALARIKRELPKEAMLLDSNFDEGGG
                     KMTFKLEVPAETATDKDFVDTIIDIVSEETKK"
     gene            11213..12076
                     /locus_tag="PKV64_02360"
     CDS             11213..12076
                     /locus_tag="PKV64_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPN15956.1"
                     /translation="MDLTKIIRKENLVYSGKSKDIYRIPGGPWKGKYAFVFTDRGTGY
                     LDKAGRPVFDPGYDSVVGEIPGKGAIAGRFATRFFNLTASKRIPSHFIASVRDEVMIV
                     EPAVPLGLPEQAPEFEGSAPLLNLEWTWRNNATGSFWRRYPFVRPCQGLDRVVEAWTK
                     GDSDTLITFESLVAAGVMTRKEVVWTEAFVKRIAAVVTEEMASRGLHLIDGKIELGRL
                     KGGDGRIVLIDEISPDVLRVCKGYAPDGKNGCRKSRTCIRTSQAGNVRKISGKNVLTA
                     AQLEEVFRTAG"
     gene            complement(12073..12747)
                     /locus_tag="PKV64_02365"
     CDS             complement(12073..12747)
                     /locus_tag="PKV64_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15957.1"
                     /translation="MARRALTAVLLLAFVSAASAAGPGRDGPFLLLSGQFLDFNRDLN
                     RNLTLWHFEKAFYVPRLERGGAWSVGYGRKSYNGSWDVSFLRLRRTVALENGSRAATY
                     EALDLNGRSFFLKKLPLRPYFLGGITLPFLRVKGGSFYRGKTLNASYVGAGLQAGAGL
                     HLDLGPSVFLNAAVTYRWIGFLYAFGEGKGRDINDLRTEPMGPEFGRLLRTDVLTLTL
                     GVGFIL"
     gene            12950..13861
                     /locus_tag="PKV64_02370"
     CDS             12950..13861
                     /locus_tag="PKV64_02370"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPN15958.1"
                     /translation="MRQYETDILIIGAGPAGLTAAIYAARSGKKTLVLESERAASRLA
                     LGYELENYPGFAAINSRELLDKFRSQAAGFGAEFATGDVIALSLESSPKFVSTSDAFI
                     EARAVILATGKPFAKERQIPGEERLLGYGVSYCAVCDGPLYKGRDAAAYGHSAEAIED
                     IWILDQTGVRVHWIPGRVKDPAALEEAFLKAEKRGIPVHGGAEIKEIAGEKNVERVVL
                     KSEGGERTLDVEAVFIFREVPTGPLFAKSGLALDHKQCLAVDRFQRTNLPGVFAAGDL
                     TCGGLQVVSAAGEGCVAALQALAYLRK"
     gene            complement(13864..17187)
                     /locus_tag="PKV64_02375"
     CDS             complement(13864..17187)
                     /locus_tag="PKV64_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014798877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPN15959.1"
                     /translation="MLRPFPSLRFRRPRPVHRSATPWAALAVLAASLLGAAPAASQDN
                     PLWLRYPAISPDGQAILFCAKGDIFKIPSSGGTAVPLTVGEAYDYSPVWSHDGRWIAF
                     ASDRSGNFDIYLMPAGGGEARRLTFHSAADIPSGFTADGRRILFASARQDTAANVQFP
                     MTGFPELYSVSVDGGEASLVLTQPAVAAVSNRAGDKILYHDAKGRENVWRKHHTSAVT
                     RDIWVYDLKAKKNIQVTDNEGEDRNPVFDPNGDDFYFLSERGGTFNIYKSSLSRPASA
                     TAVTSFAKNPVRFLTRSDTGVLCFGYDGEIYTQAGDAAPQKLAVRIAQDGRAVLPRVL
                     PVGGSGLTEMRLSPNGKEIALVFRGEIFVVSAEGGPAKRVTDTPEQERMVSFSPDGRT
                     LIYAAERDNNWNVYATSIVRKEEPYFFAATLLKEEPVAATAAEEFQPEFSPDGKEVAY
                     LENRTALKVINLATKQSRLILPGTYNYSYADGDQSYRWSPDGKWFLVQFGVVRLFTPQ
                     IGLVSSDGRGRVINLTRSGFDNVGVRWGLDGTMMYYGSTREGLTNTDGNPMTYDIYGM
                     FFTREAYDRFLLSKAEFALLKETEQKAKDEKEKTEKEKAADAKAKAAGLKKEEPKKEL
                     AFELDGLDRRKVRLSIHSADISDAVLSKDGEKLFYLARFERGYDLWVTEPRTRDTKLL
                     AKLGVQRPSMELSPDGKTLFIGAEGRILKVDPDSGRQEPLAITGEMRLDEAAEKAYMF
                     DHMWRQIKQKFLVEDLYGADWDSFYPIYRKFLPFINNNHDYAEMVSEMLGELNASHTG
                     CYYNPARTPSADATASLGLFLDYDYAGPGLKVAEVLSGGPLDKASLKIRAGHIIEKID
                     GRTLDGTIDHYALLNRKAGQLTLLSVLDPAASSRWEEAVRPVTLEEEAALLYRRWVLA
                     RRAETDRLSGGRIGYVHVRGMNDQSFRTVIDEVFGLSMEKDALIVDTRFNGGGSLHDQ
                     LADFLNGRKVFDIVPRGQLVGYEPYNKWIKPSIVLMGEANYSDAHLFPVEYKIKGIGQ
                     TLGMPVPGTGTFVWWETQIDPTLRFGIPQGGWRTPDGKLCENNQLEPDIRVKNDPDVM
                     SAGRDQQIEAAVRELMKGK"
     gene            17295..18464
                     /gene="iadA"
                     /locus_tag="PKV64_02380"
     CDS             17295..18464
                     /gene="iadA"
                     /locus_tag="PKV64_02380"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HPN15960.1"
                     /translation="MAIIIRGGSVFSPGADGARDILVAGGRIEAIAGPETVVCRGFET
                     TVIEAGGKSVLPGFIDPHVHILGGGGEGGPATRAPEIAVEDIVAGGITTVLGCLGTDS
                     VTRHMSSLLAKARALEIEGISTRCFCGSYEIPVKTVTGSVRGDLVLIDRIIGAGEIAL
                     SDHRSSQPSFEEFARAAAECRVGGMLGGKAGILHVHMGDGARGLEYLRRLVRETEIPV
                     TQVVPTHCNRSRRLLEEAVEYAKSGGRIDVTAGTDPDRESDHDVSVETAVRLLINGGV
                     PLERVTVSSDGNGSLPVFDAAGNLTGLTVASPRALLQTFRSLVGRKILTLEQAARLFS
                     ANPASIYRLEEKGAVAPGRDADLLVFDKELQLTDVIARGRVLMKDGKLLVKGAFR"
     gene            complement(18562..19962)
                     /locus_tag="PKV64_02385"
     CDS             complement(18562..19962)
                     /locus_tag="PKV64_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HPN15961.1"
                     /translation="MKKPRIPHTLILVYAMVVLTVAATWIIPGGEYQRVEKDGRLVPV
                     AGSYQRIESRPQGLGGLFVSPARGFIDAAAIIGILLIVGGAFSVIQKTGAITVVIHNI
                     ACLFGRSRALRVLLIPVTMTVFSLGGAVFGMCEETMPFILIFVPLALSLGYDTIVGVS
                     IPFLGAAAGFAGAFFNPFTVGIAQGIAGLPLYSGMGYRLIIWAGGTAIAVVLVMRYAA
                     RVLKKPEISPVFADDLEKRKKLLGTTPAMEKLPVLLRHKLVLGVFAAGFGVMIFGILK
                     YRWFINEIAGIFLAMGILSGILGRLTGDEFGKAFVDGAKDMVNAALIIGTTRAILIVA
                     TDGRIMDTILAGMASAISNLHPVLSAQLMFLSQCGINFFIHSGTAQAALTIPVMAPLG
                     DLVGITRQTSVFAFQLAEYINPILPTSGVTMGVLGLAGLRYEKWAKWLIPLVIAWVIF
                     GLLTLIPPVLFKYGPA"
     gene            complement(19959..20825)
                     /locus_tag="PKV64_02390"
     CDS             complement(19959..20825)
                     /locus_tag="PKV64_02390"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HPN15962.1"
                     /translation="MKKIVFALVLGLLVSFVLPAADKGSLLIIGGGDRPDGLTEKFVA
                     LSRGFGAGKIVIFTMASGVPEESGPETAAEVKAAGAPDASWFHLTREEALRPESAAIL
                     EGAGGVYFCGGDQSRLTAVLLDTPIHQRLLELYGRGLVVAGTSAGAAAMSEMMITGNE
                     KRTREEDENGRTIEAGNVETVRGFGFITSAVIDQHFVTRRRHNRLMSVVLENPGLLGV
                     GIDESTAVWVRPDGKYEIAGLRQAVVYDARRASGSRTADGLLGAAGMTIHILLPGDVY
                     DPAIGAVQGGRK"
     gene            complement(20944..21390)
                     /locus_tag="PKV64_02395"
     CDS             complement(20944..21390)
                     /locus_tag="PKV64_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPN15963.1"
                     /translation="MSMAEELAARVIWKGKLEFEGRTDFGQPIAIDVPPPEGDDHGAK
                     PMELLLISLASCAGQIIVSLLRKMRQDVRGFSLTAKGLKQAEHPRVFTSIRLDVLVSG
                     PGLDPAAVDKALQLAEDKYCPVFAMLKKSVDIRTEITVSGVSDPPA"
     gene            21617..22690
                     /locus_tag="PKV64_02400"
     CDS             21617..22690
                     /locus_tag="PKV64_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPN15964.1"
                     /translation="MMVRFKDSTADELHAALAPRGISLRLARRLQAAVLRRDAFPRSL
                     PEVSEKTLAWVRENAGLPKLVQKGKAVSARDGFAKYLFQGEGPEPFEAVRIPLLHRAG
                     EEKVVACLSSQVGCGLGCAFCATGRMGLVRNLSAWEMVDQVIRLAADSEHPVRGAVFM
                     GMGEPMLNYEAVVRAARILNEPCGLAISAEAISISTAGIVPGIRRFTADRLPFRLVVS
                     LTSADPFRRRGVMPLERTHPLPELMEALREYHKASGQRVILAWTMISGFNTREEDAIQ
                     LAALVRGLPVRLDLIDVNDAAGRFKPPSPPELAAFRDALRKHLKMPVARRYSGGRDIH
                     AACGMLAGSLPGPSRRESRSPAS"
     gene            23025..25346
                     /locus_tag="PKV64_02405"
     CDS             23025..25346
                     /locus_tag="PKV64_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPN15965.1"
                     /translation="MKKSRRTFVCVFLALTLLLPVFSSAQGTRADYERAGGLPEKWKN
                     LAVNQPDQPEWIEKTHKFWYRKSTAAGYEFVLVDADAATKTAAFDHEKLAGALTEALK
                     DRIDPKNLPFTRIEYVDQMKAIEFESGESRWKFDLAAGTVVRISRVLSRWSDESNWQD
                     VGGRAEPAASAEAKASPDGKWEAFVRNYNVYLRPAGGAPGGGPQTAGFALSLDGMESD
                     YYSFRSITWSPDSRNIAAFRVRRGPHRRIDFIESSPDGQLQPKTFSLGYTKPGDVIDF
                     LRPVLFHVDERKAVDIDNALFANPYDLSELQWREDGRAFTFEYNQRGHQVYRIIEVDA
                     ATGRPRAVISEEPRTFFPYLEISSYGKKFRYDAADGREIIWMSERDGWNHLYLFDGAT
                     GQVKNQITKGEWVVRGVDKVDETARKIWFKASGMNPGQDPYFTHHYSINFDGTGLTAL
                     TDGDLTHQAAFSSDMAFFTDLSSRHDRAPILDVRRTADRGLVLRVEQTDISGLVKAGW
                     KAPEAFRAKGRDGRTDIWGVIYRPLNFSSKKKYPVIEYIYAGPHDSHVPKTFVGFSPL
                     QALAELGFIVTQVDGMGTSNRSKAFHDYCWKNLADAGFPDRILWHKAVAAEYRYYDIT
                     RVGIYGWSAGGQNALAALLFHPEFYKAAFSSCGCHDNRMDKLWWNEQWMGWPIGPEYA
                     ASSNAENAWRLQGKLLLLVGEIDTNVDPSSTMQVADALIKAGKEFELVVLPGVNHTSG
                     GTYGDRKRNDFFVRNLLGLEPPDWNAPGPPANK"
     gene            complement(25349..28648)
                     /locus_tag="PKV64_02410"
     CDS             complement(25349..28648)
                     /locus_tag="PKV64_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPN15966.1"
                     /translation="MKKTAALILSVLFVFSLAGWSRKTEADSRKAVPDKTPLLTSELL
                     SGLAFRNIGPAVMSGRISDIVIHPKRRATWYVAAGSGGVWKTENAGTTWTPVFDGQSS
                     YSIGCLALDPVRPEIVWVGTGENVSGRHVGYGDGVYKSLNGGMTWTNMGLKNSEHIAR
                     ILIDPRDPDVVYAAAEGPLWSPGGERGLFKSVDGGRTWTPSLIISADTGVASAEFEPG
                     DPDILYAAAYQRRRSVAAFMGGGPESGIYKSEDAGKTWRKLSVGLPKGDMGKIGLAVS
                     PLDPRVVYATIEASPEERGFYRSADRGESFEKRNSYLSGGTGPHYYQEIFADPNVFDR
                     VYQMDYWLQVTDDGGRTFRTVPEKNKHGDNHALAFLPGDPDYLLNGSDGGVYETRDGG
                     RTWRFFENLPVTQVYKLALDNDLPFYNVHGGTQDNGSQLGPSRTLNANGIANFDWTIT
                     FGADGYACAIDPVDPDTIYLEWQEGNLLRYDRKSHETIFISPKPEPGEPALRFNWDSP
                     VLISPHSHTRLYYAGQHVWRSDDRGDSWTRISPDLTRNIFRLAQPIMGKTWSVDALWD
                     HGAMSLFSTITTLSESPLAEGLIYAGTDDGLIQVTEDGGKSWRKIDRLPGVPENFFVN
                     EIKASRTDRDTVFAAVDLHKTGDYRPFLLRSDDRGRTWVSISGDLPARTIVWAVAQDP
                     VKKDLLFAGTEFGVFATLDGGKRWLKMAGGVPTISFRDLEIQEREGDLVGASFGRGFF
                     VLDDFSPLRRIDETLLARPAALFPVKKTLSYIPRRPIDSPDKGCLGETFFTAPNPPFG
                     AIFTYYLKDGLKPAAQARRDEEAKFLKEGKPVSFPGWDVLRKEEDEEKPEIILTVAAA
                     DGSVVRRLTGPAGPGLHRIAWDLRYPAVEPTRLESAVRDPWDMEPMGPLVVPGTFSVS
                     LAQKIDGVLIPLAGPETFTVESLTLTSLAEKDKAALLAFQEKAGGLQRAMMGAGEAAD
                     SALRSLAYVRKALLDTPAADPKLAETARAIETGIREALRDLHGDVTLMRRSEARTPSL
                     LDRVSRQLGSTGPLTETVKKDYAVAADAFGAVLEKLRGLIDGDLRRLGEALEAAGAPW
                     TPGRPVPVWKK"
     gene            29002..30543
                     /locus_tag="PKV64_02415"
     CDS             29002..30543
                     /locus_tag="PKV64_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPN15967.1"
                     /translation="MSGHGIFAGDIPRRKFLEMGVKGGLAIAASPILLKDLLAKTGPG
                     DLPAVGPALDKAVLDRVVARALAKGGEFADVYVEHRISRNILMEESKFRSAEFGISQG
                     AGVRVIAGDKTGFAYTDEIGEEAMLRAAEVASFIARGSAAAKPVPIRAGAPPVYITVK
                     LPLSGIADEKRLDVCRRADQAARGHDPRIKMTSISYYDEVRGRTIANSEGLYLVDELP
                     LLFFIVQALSEGNDTRHMGRERLSRHSGFEMFAEVTPERIALTAAREAVAMLEAKDAP
                     AGKLDVVMQNGWGGVLVHEAVGHPLEADNIARKIGAFTGKLGQKVASDVFTMIDDGTL
                     PNFRGTTNWDDEGTPGKRNVLIDKGVLVKYMTDVLSAKQIKMERTGNGRRESFRYLPI
                     PRMTNTFIAAGKDKPADILASTKNGIYVQALSGGSVDPTTGVFNFTCREAYMIEDGRK
                     TTPVKGATLIGNCLDIIQNIDAVGDDLEFGPGICGKGQSAEVTAGQPTVRLRGINVGG
                     SRARA"
     gene            30568..31902
                     /locus_tag="PKV64_02420"
     CDS             30568..31902
                     /locus_tag="PKV64_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPN15968.1"
                     /translation="MDMKILAEKLVKDCLKKGADAAEVYIETGRSLSLEVRNGEVETI
                     QEAAGQGVGFRVFVQGRMALAGSNDFKDSALDDAIGRAIEFARVTTADEFNVLPDDPG
                     QTAVEGLYDPEIARVSTDRKIELLKTLEKLSMKDPRITKSAGAGYGEGENEVFIANSH
                     GLSKTTKGATCAMGISVVAEKGDQKSSGGEYCSRRFWADLKPAEEIAAAAARKAIELL
                     DPRPLKTQKAAVIFDPDVAYAVLGGLLQAVDGERVLQGASFLGAKLNRKIAVETMTLV
                     DDGTRPRGLASVPFDGEGVPTQRRLIVDKGVLRGFLYNTIVAKRAGVKSTGNASRGGF
                     SSIPGIGPHAFYMEAGPHKPEEIVKATKAGLWLKDVTGYGINPVNGNFSGGASGLWIE
                     DGKVAFPVKGVTIAGTAEEMLNGIDMIGDDLDLNRGLTAPTFRIALMQIGGE"
     gene            32285..32899
                     /locus_tag="PKV64_02425"
     CDS             32285..32899
                     /locus_tag="PKV64_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HPN15969.1"
                     /translation="MEIQLQELLDRIKREGQEEAERRAAEILREAEERKKTILAEAER
                     EANEIRTKAKTAAARDEEAGRASLARASRDLVLAFRDKIAAILDAVVRNAVAAAYDEE
                     ILRAALPDVLKSLAAGGAEDLSVLLDPEMLRKVRDRFDARLKEELAKGFTFTPFPGVP
                     AGFRVAEKNGAAYYDFSADAVAELFSKYLNSRLAGIVREAVKDL"
     gene            32903..33487
                     /locus_tag="PKV64_02430"
     CDS             32903..33487
                     /locus_tag="PKV64_02430"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HPN15970.1"
                     /translation="MGRYYYFASTLPALSLGAPPPLSSADFLRRGRLHLRPMDFALLE
                     KTTLQSGPDLPPPAGSGLLDRYYAWERSLRNELVSLRAARLGRSGDRWRRPAGRDDDA
                     ARDARSVFLAVPDASTPLEAEIALESVRWMRIDRLRGLTVFDFDSLLAYRLQLLILER
                     LAVLTAGRGERNYQEIYDAVKEAARTPEQTGETP"
     gene            33484..35253
                     /locus_tag="PKV64_02435"
     CDS             33484..35253
                     /locus_tag="PKV64_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPN15971.1"
                     /translation="MTKTSGTVTGVNGNMVTVFTQGTVTMNEVAYIHVDDTRLKSEVI
                     RVQGGTVQVQVFEITRGIKVGDPVDFTGELLSVELGPGLLGQIYDGLQNPLPQIAEKT
                     GNFLTPGVYLEALPRSRKWAFTPLVQAGGRVERADALGTVPEGAFSHRIMVPFSLAGA
                     WTVAEIKPAGEYAVDEPVARLTDDGGGSVDVTMRFTWPVKRAVDCYVQRLRPVQPLVT
                     KTRLIDTFFPVAKGGTYCIPGPFGAGKTVLQQVTSRHADVDIVIIAACGERAGEVVET
                     LKEFPALIDPRTGRSLMERTIIICNTSSMPVAARDASVYTAVTLAEYYRQMGLDILLL
                     ADSTSRWAQAMREMSGRLEEIPGEEAFPAYLESVIAAFYERAGVVRLKDGATGSITIG
                     GTVSPAGGNFEEPVTQATLKVVGAFHGLSRERSDARKYPAIHPLESWSKYDGIIDGPI
                     IDYGRAFLRQNAEVGQMMKVVGEEGTSLDDYIVYLKGDLLDAVFIQQNSFDAVDAAVD
                     PPRQKDSYRLIMEILAAEIKAKDKEDARRWFSGLRQKFLDLNGAPWQSERYAALSEEI
                     RALLAERRGGDDEKGLRLIEALE"
     gene            35257..36558
                     /locus_tag="PKV64_02440"
     CDS             35257..36558
                     /locus_tag="PKV64_02440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPN15972.1"
                     /translation="MKKVYSRIESITGNVITVRAEGVRYSELASIETRFGSSLAEVIR
                     LDGDLVSLQVFQGGRGVSTGDSLRFLGHSMRVSFSDNLLGRIFDGAGEPRDDGPRLSE
                     NLIPIGGPSVNPSKRIIPREMIRTGIPMIDLFNTLVKSQKLPLFSVSGEPYNPLLARI
                     ALQAEVDVIILGGMGLTHDDYLFFRDALEQGGALAKAILFVHTAADPVVECLMVPDLA
                     LAVAERFALRGKNVLVLLTDMTNFADAMKEIAITQEKVPSNRGYPGDLYTQLAARYEK
                     AVDFADAGSITILAATTMPGDDVTHPVPDNTGYITEGQFYLKNGRIEPFGSLSRLKQL
                     VNAKTRPDHRPIMDGMIKLYASYKETLEKRAMGFLMTSWDRKLLRYGELFEREMMDLS
                     VNIRLENALDKGWQILAACFEPLETGLRTELLEKYWPKKTA"
     gene            36569..37186
                     /locus_tag="PKV64_02445"
     CDS             36569..37186
                     /locus_tag="PKV64_02445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPN15973.1"
                     /translation="MAKVKMTKNELKRQRDALKTYLRYLPTLQMKKQQLQVELRIVEA
                     RRRELRTAREELDREFRTWIAVFGESSALCGPDGRPFLSVAAVRTSRGNVAGVDIPVF
                     EGADFAVAEYDFFTAPLWVDAAVERMKKILLVDLEDDVLAGQAGRLAAELRTTSQRVN
                     LFEKVKIPETLENIRIIRISLGDQQIAQVVRGKIAQRKVIARTAA"
     gene            37183..39009
                     /locus_tag="PKV64_02450"
     CDS             37183..39009
                     /locus_tag="PKV64_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15974.1"
                     /translation="MIARMKKVWLVLLEKEKAGALRELRDLGVVHVECVRPSGGRFER
                     LARTRDEVAAALNIIPAEKRLAPGRLSIDEALALARRILAGEERVREIQDAGAALQRE
                     IDRAAAWGEFDPEAIEGLRASGTDIRLFEVDPKKRATAPAGTDIVVLSADRRRLLAAA
                     VGRRGEPVPPLPPAFVEFVPPLLSAAAMREKIAAGEAEIAEIGKKRVAAGRERDSLQR
                     ALEILEQDLSFETVRESFIGEGPVCHVKGYVPAADLGALSVRAKDHGWAFAAADPEPG
                     DQVPTKVENGRFVRMIEPVFEFLGTVPGYHEFEISFWFLVFFALFFAMIFGDAGYGLL
                     MMGAGLAAALKARRKSRTVPDGVRLLLFLSGALILWGAATGNWFSIPFASLPPALRAI
                     AVKPICIANPDAKANIQVFCFLVGVVQLSLARIKNIRRLFPNLQFLAHLGSLAMIIGM
                     LFFVLNLVVDAKRFPLPAYAVGLVVGGFAANLLFGAYQGNLLKSLLGGLQNFIPLFLG
                     TVGVFADIVSYIRLWAVGLAGSSLGSVINGMGGGLIKPALTIIAGVLLLTFGHALNIA
                     LSVLSVVVHGIRLNMLEFSNHLGMEWSGIKYDPFRVTAKSER"
     gene            39028..39456
                     /locus_tag="PKV64_02455"
     CDS             39028..39456
                     /locus_tag="PKV64_02455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968952.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HPN15975.1"
                     /translation="MDLIGYIGVAAVLGLAAVGSGIGAGIAGQAAIGAWKRSYLANKP
                     ASFLLVVFAGAPLTQTIYGFIVMNALKGAGIDGLFKLGIGVLSGAAIGYSAVAQGQCS
                     AASCDAFGETNKGFAQDLIVVGLCETVAIFVMAFSLAIIG"
     gene            complement(39446..40036)
                     /locus_tag="PKV64_02460"
     CDS             complement(39446..40036)
                     /locus_tag="PKV64_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPN15976.1"
                     /translation="MPGKSAAARSKRLMSAERRQEIPPERVLETIGLRPGQVFIDIGA
                     GPGFFALPAAAAVGPRGRVVGLDVSPSMVEELRKNAARAKAANLQARRISKTSPNVPP
                     GADFYFLANVLHEIDGREAYLRDLRKRMSAASRLVIIDFLRKKTEHGPPLRDRIPLRE
                     LRRLLAATGFKVERVFRPNGEEYGVIAFRTGARLNR"
     gene            complement(40319..42229)
                     /gene="rlmD"
                     /locus_tag="PKV64_02465"
     CDS             complement(40319..42229)
                     /gene="rlmD"
                     /locus_tag="PKV64_02465"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HPN15977.1"
                     /translation="MDVILASRSPRRKEILGRIFPDFRVIPSGVDESALDQSDPVFFV
                     REAAVLKARDVGARFPDSLVIAADTVVCLDREIFGKPETREDAAAMLGRLSGRRHRVL
                     TGVALFRAADDRLVVGYETTHVTFKALAPEEIEAYLDRNAYADKAGSYAVQDVGDAFV
                     ERLEGDYDNVVGFPVKRFKKLLKEFKAPEMELEITGIAFPKSWPVARAGEKDVWVPGA
                     VVGDRVRARILRRNPAVAKITRLLEPSPLRTEAVCPHFGACGGCAFQNLNYSEQISLK
                     EGYLRRVLGKEGPPGAAEVLEPFLPSPSLFGYRNKMEFAFAGAGGDLRLGLRERSLPR
                     TKSRKRTVDLRTCPLFGPAADRLLPAAAAFAEADGRPGFDPVSRKGFFRNLVLREAKG
                     TGEVMVILVTRSGPAPGLREWAGRLAADIPAVRSVWHVENDRDADLVEYGNNTLLVGA
                     AGIEEELSGLSFRIGPDSFFQPNPKGAAVFYEKIAARAVEVSAKKALGLFCGPGAIEM
                     FLSRTAAEVVGVDAEPANIQSAEGNAVRNGLRNVRFIAGLVEKVLESTDLSGTDLVVL
                     DPPRDGLHPAGLTLIPALGAPHLIYMSCNPASFARDIRGLAASGYRPERIFAADFFPH
                     TPHFEVLGFLSR"
     gene            complement(42235..43293)
                     /locus_tag="PKV64_02470"
     CDS             complement(42235..43293)
                     /locus_tag="PKV64_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPN15978.1"
                     /translation="MKKPTSPRRARTAALLLLLGGPILLHSQQAPLTEDERNTIEVVN
                     KTRNSVVYITNIQLVRDFFFGAEEKIPRGSGSGFVWDRRGHIVTNFHVIEDGVEFQVT
                     LPDQQQRSARLVGKDESKDIAVLRLDGDVSELSPVVPGTSRDLVVGQKTIAIGNPFGF
                     DYTVTKGIVSALGRKITGAGGVSIRDMIQTDASINPGNSGGPLLDSAGRLIGMNTLIY
                     SPSGASSGVGFAVPADIIAKVVPELIAHGRVTRPGLGVSFLNDAYARRAGIEGVIILE
                     VPSGTPAYAAGLRGLGRDRFGRRTINDVITAVDRAPVTSYDDLFAILESRKIGETVTL
                     SVDRAGKVRPVRVTLVRD"
     gene            complement(43351..44337)
                     /locus_tag="PKV64_02475"
     CDS             complement(43351..44337)
                     /locus_tag="PKV64_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15979.1"
                     /translation="MTNNLDAYLEDIGHYLAVGPAREEILSEIRSHILEKAEEERARS
                     GEAALERVIAGFGPPRRVAERYLEGREIIAPAYQRHLFRYTSILFGFHFLFTLIGVIF
                     GRSFVLFPVLFVPRMGVIEALLYLPAAFLTDLGFVALILYFVTQSKKDIKLPWPKFGL
                     EADDIKLPRKPGALAATLAGLTFMLALTAAAVYVFTRHGTVFIAGLASSGEIKPLFTA
                     EAGRSLSLIVIALLAAGAVSLFLKLFTRSRWIDVGSDAVSLILFGLILRRPFDGLFAV
                     PISGQALSGIRIGLAAALLFIVLMTAIDLIKNLVMIGRGALVRRPPERGTPA"
     gene            complement(44327..44671)
                     /locus_tag="PKV64_02480"
     CDS             complement(44327..44671)
                     /locus_tag="PKV64_02480"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPN15980.1"
                     /translation="MTETELGSLETEMNRGFLQILVLALLEKDRYGYSMIKHIEGLGY
                     KIEENTLYPLLRRLEKNGWIAGRWTLGEDRPRKFYGITAEGRAMKAGLLAVWKNQNRI
                     LEEIEKENEHDQ"
     gene            45181..45819
                     /locus_tag="PKV64_02485"
     CDS             45181..45819
                     /locus_tag="PKV64_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HPN15981.1"
                     /translation="MLDVIVKLVLAVVLGGVIGLEREFGQKPAGLRTNILICAGSTMM
                     MALSGIILGGAPVGSADSLRIASGVITGMGFIGAGTIIQAHRHVHGLTTASTLWAVAG
                     LGLVIGAGYFLIAAIYTGILTIVLLSFPLVEHLVPHRSIVRYNLRVAGIEAVDELRGL
                     AAATGEKVDEIFFRRETNGAVLVFVITERPSRARTFRDKIAALGEIVEIRID"
     gene            complement(45816..46706)
                     /locus_tag="PKV64_02490"
     CDS             complement(45816..46706)
                     /locus_tag="PKV64_02490"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN15982.1"
                     /translation="MTKRLSSFAVPAALLLLISGAAGQVRLLDVPYVPTKFPVVDEML
                     SMAGVSKNDIVYDLGCGDGRIVIGAAQKYGARGIGIDLDPERILESEANASSAGVADR
                     VRFYTGDLFEADIREATVVSLYLLTSINLRLRPRLLRELRPGSRVVSHNFAMENWKPD
                     ASSEVEVDGVRHDVFLWVIPANASGAWTWTMKIKGKTTTVRADLIQKYQYAKGTVKIG
                     GTEAAIADAKIQGDLVRFRFEAAVAGSPAAFVFEGKLVGQSINGTIRSVVDGETRVWT
                     WKAFRNPTTEKPIDEPPDAG"
BASE COUNT         8612 a        14653 c        15298 g         8195 t
ORIGIN      
        1 tcgatcatgc cgggcaaggt gaacccggtg attcccgagg tcgtcaacca gatcgccttc
       61 aaggtcatcg gcaacgacct gaccgtcacc ctcggggccg aggccggcca gctgcagctg
      121 aatgtcttcg agccggtgat cgcccagagc atcttcgaat ccatcgagat gttgaaaaac
      181 gggatggaca ccctccgtct ccggtgcgtt gacggcatca cggccgacga agcccaatgc
      241 cgcaagtccg tcgagcggag cttcgccctc gtcacggccc tcgtgcccga gctcggctat
      301 gaagcctgct cggccctggc cgaggaagcg atggccacgg ggcggagcgt ttacgccgtc
      361 gtctcggaaa aagggctcct gcccaaggaa cggctggacg agatcgccaa gccggaaaac
      421 atggttcggc cccggacata agggaaaaaa agggaatcgt gtttcttcgg ccccggcgac
      481 tttccggctt atccggggcg tgtttgaaaa tcgctgtgca ttatgattaa ataaggccct
      541 ttacaaggag aacccatgga catttttgac aagtgcaaga cggacggcgg ctattttggg
      601 atgtttcggg cccggaacga ttttttcttc acccgtccca tcctcgatcc cctgcccggg
      661 cgggagatga cttaccaggg gaagaaagtc gttcagtggt cgatcaataa ttatctcggc
      721 ctggccgaaa acgaggaatt gaaagccatc gccgtcgagt cggcccggaa atacggcccc
      781 ggcgccccga tgggcgcccg gatgatgacc ggcaacaccc agaagcacct cgacctcgag
      841 gccaagttcg ccaagttcat gggcaaagag gacgccatcc tcttcaatta cggctacctc
      901 ggcgttctgg gcaccatcca ggccctttgc gaaaaaaacg acacgattct gatcgataag
      961 ctggcccacg cctcgatgat cgacgccatg ttcatgtccc aggcccagtt ccgggtttgg
     1021 aagcacagcg atctcgacag cctcgagctc catctgaagc gggtcaaccg ggatcgcaag
     1081 ggcggcatcc tggttgtcac cgagggcgtc ttcgggatga ccggggacat cgccccgctg
     1141 tccgacatct gctacctcaa ggataagtac gacgcccgcc tgttcgtcga cgacgcccac
     1201 ggcttcggcg tcatgggacc gggggggagg ggcacgggcg agcttcagaa atgcctggac
     1261 aagatcgaca tcaacctcgg cacgttcgcc aaagcgttcg ccgccatcgg cggcgtggcc
     1321 ggctcgaccc acgaggtctg cgagtgggtc cggttcaacg cccggaccca ggtcttcgcc
     1381 aagagcctcc cgatgatcta tgtcgacgtc ctttcccggg ctctcgattt tgtggccgac
     1441 ggagaggccc gccgcgcccg gatgtgggaa gtcaacgcca agctgaaagc cgggctccgg
     1501 gagctcggct atacggtcgg cgaggtgcct tcgccggtca ctccggtcct ggtccattac
     1561 aaggacttgc atatcgccat ggccgtcctc caaaaaatgc gggaggaggg gattttcctc
     1621 accggagtga tctacccggt cgttccgcgg ggcatcgtcc tgttccggat gatcccgacg
     1681 gcctcccata ccgatgagga catcgcccgg acgctggccg cctataagaa ggtgcgcgac
     1741 gagctccatc tcgacttggg cgccgtcaag gacgccctgg cccagtacga aatcgaaaag
     1801 aagtccgcgg acaaggacga ggacgaaaac aaaaccgaaa tatgattcag ggacccgttc
     1861 tcatcaccgg ggcgtccaag ggcatcggcc gggctcttgc cgccgccctg gccgcccggg
     1921 ggatcccggt catcgggacg tcccgccgcc ccgaaaccat cgccgatccc gtccccggcg
     1981 tccggtacct gaccctcgac caggaggacg acgcttccgt ggaggcttgc gcggccgcgg
     2041 ccggtccggt ggaagccctg gtcaataacg ccggccaaag ccagatcgga gccgtcgagg
     2101 acgtttcgat ggagacggtc gaggagcttt tccggatgga tttcttcggc ctcatccggc
     2161 tgacgaaggc cgtcctcccg gggatgaggg aacggggccg cgggacgatc atcaacatcg
     2221 gttcgctgac ggggacgttc cccccgcctt accaatccgc ctatgccgcg atcaagctcg
     2281 gcctggaggc cttcaccaag gtcctccgcc aggaggtcgg ccggcagggg atcaaggtct
     2341 ccctggtcgt tccgggttac atcaaaacac atatcgagcc gagaacgatc gctccggcgg
     2401 aaagcgtcta cgcgaccgat ctggccgcct tccggctggc ccgagacaag aaaatgggca
     2461 aagcctcccc gcccgaggcc gcggccgcca agatcatcaa ggtcatggag atgaaaaatc
     2521 ccgggccggt ctattacacg gggcgccttg ttccggcgat gggcttcctc aagcgcctcc
     2581 ttcctgaaaa gaccgcccag aaaatgatcc gggggtttta tcacctggac ggctgagctt
     2641 cgtcctttcc cggcccgcct tctcgggggg cggatcggag aagccgtcat ttcctcgcgg
     2701 ctttccctcc cgcgcacgaa acgagtcctc ctcgtcgctt gttcgcgttt ttcctgtccg
     2761 cggctttaaa aagagaaccg ttctcgaagc cagaggacgg aaggatcccg cttcccgatc
     2821 agccgcttct ccggatcgtc gggaaaacgg ccgcggcggc gcgtgcggcg gatctcccgc
     2881 cggagaacgg cggcccgggc gttcgctttt caagcgcgga taatacgttg acgtttcctc
     2941 ttgacatttg tcttctgata aaatatacgt gtacgtatag atatggttgg aaacaaacta
     3001 aaataaacgt gagggtatgg tgggcaaaga aatgatgacc agggcggagc tggcggaaaa
     3061 gacgggtatc gagcctgtgg aaatcgatct ttggacgaaa gccaagatcc tccggccggt
     3121 cggcttcgcc gaggacaagt cgccgtttta ttccggcccg gacatcggcc gcgtcgccca
     3181 cgtccgcaag ctccgggagc tcggctacgg cctggaggaa atccagaaga tcatcaagaa
     3241 atacggctgg cccgggagcg gagacgatca gaaaaaagac gccggcaagg gcaagctcct
     3301 gaccgtcggc ctgctggccg aaaaagccgg cgtcagtccc cggaccatca agcactggga
     3361 ggacgtcggc atcctcgatc ccgatctgcg aagcgagggc ggctttcgtc tctatgatga
     3421 aagctatgtc tatctctgcc agcttgtccg cgatctccaa ttgttcggtt acagcctgga
     3481 ggagatcaag gcggtttcgg acgccgtccg ggatttcctg tctctccaga aggacctctc
     3541 cgtctactcc cgcgaggagg ccggccgcaa gctcgatggc cttcaagccg cggccgaagc
     3601 ccttttcaag aaaatgaggc cgctgcggga aggtatcgat cgttgggagg acttgttgaa
     3661 aaagaacaag aaggagatcg ccgccctcaa ggccaagaac gccaagcggc cgccggccgc
     3721 ctcggagaaa acccatgcct aagatcgccc tgatcgagcc ccggtcgccc aacctccata
     3781 tcttttccaa gtttccttct ccccgcctcg ggaccgtcct gttggggacg atcatgaaac
     3841 agcggggctg ggacgtcgag ctgtttgtcg aagatttccg ggcgcttgat ttcggcgtga
     3901 tcggaagcgc cgacatcgtc gggatttcaa cgatcacgtc gaccgccccc cgggcttatg
     3961 ccatcgccgg gcgcgtccgg gagatgggga ttcccgtcct tttcggcggg ccgcatgtca
     4021 cattccttcc gggggaaagc ttggagcacg gcgattacgt cctgcgcggc gaagcggagg
     4081 agacgctccc cgcatttctc gaggcgtgga ggacggaggc ggacttgtcg gaaatcccgg
     4141 ggctttcctt ccggcggggc ggcgaagccg tccataaccc ccggtccgcg cctccccgga
     4201 acctcgacgc ctttccttac gcggatttcg acctgctcaa acccgaccgg cggtggcggg
     4261 ggaaaaagat ggtccttccc gtccagacgt cccggggctg cccgttcgat tgcacgttct
     4321 gttcggtgac cgggatgttc ggcaaagctt accggttccg gtcgaccgaa catatccttg
     4381 gggagctgcg cctccacgcg gaccgaaacc ggaatctctt cttctacgac gacaatttcg
     4441 ccgcccacaa gccgcggacc cgggagctcc tcgaggcgat gatccgggag ggattcaagt
     4501 tccgctggac gacccaggtc cgggccgacg tcgcccgcga tccggatctc gtccggctga
     4561 tgaaaaaagc cggcggccat accgtctgca tcgggttcga atcggtgaat ccccggagcc
     4621 tccaggaatc ccataaggcc caatccgtgg ccgagctcga ccgggccgtc cgggtgttcc
     4681 gtcgcaaccg gattcatatc cacggcatgt tcgtctttgg attcgaccag gacgaccggg
     4741 ggacgttccg ggagaccgtg aagtgggccc ggaaatcgcg cttgacttcg tcgcagttcc
     4801 tgattctcac gccgctgccg ggaagcgcgt tctataagaa agtgatggac gaggaaagga
     4861 tcgccttcaa ggattggtcc ctctacgacg cccatcacgc cgtcttccgg ccgagacggc
     4921 tgtccctggc cgagcttcaa aaagcccaga tcgccggcca tcggcggttt tattccttga
     4981 aagagcaggt tcggaacgtt ctcgagggcc gttggctggc catcgccatc aaccaatacg
     5041 cccgggcgct caaccggacc tggcagaaaa agaaccggac gtttctcaag gctttggatc
     5101 ttctccgccc ccgccgggga agccgggtct gggtggatta ccggcaggac gtcggcctgg
     5161 attaaggaat cgggccggct cgggcgggct tacaaaatcc gccgggaggc ctgatagacg
     5221 gcgatgccga cggccgcgga gaggataaag agataggcga cattgaacaa cgtcaggcgc
     5281 caggagcggc tttcctgctt gaaggcggcc accgtggccg cgcagggaat gaacaggagc
     5341 tgcgaggcca tgaaagccag cccgaccgcc ggaggatagg cggatacgat cagatctcct
     5401 aaagcgaccc cctccccggc cccgaagaga acagccaggg cggcgaggga gttttccttg
     5461 gcgatgaatc cggcgaaaag ggcgaccatc agcctccagt cgaatcccat caaggttccc
     5521 gccggctcca gggctcggct gaatccggcg agatacgagc tttccaccct cccttcgggg
     5581 aaacgggaga gggcccagac agccagcgag acgggcagaa taacgaccac ggccttgcgg
     5641 acgaattcaa tgcttcgccg ccggacgaga tcgcagatcg tccggggatg ggggacgtgg
     5701 taaagcggca gctccatgat gaacggggcc gcgtcctggc ggaaaaacag ccggccgccg
     5761 atcatcccgg tggcgaaaag aaggcccagg gcgagcagaa tgaggccgat ggcgaccagg
     5821 gccgcctgtt cccggaaaaa cagcggagcc agaaaggcga taacggccat tcgcccggta
     5881 caggggatga ggggaatcag gaacatcgtc aggaggcggg cccgggtcga ctcgatgatc
     5941 cgggtgccca gcactcccgg cacattgcat ccgaagctga tgatcagggg aaggcaggat
     6001 ttcccgtgca gacgcatggc gtgcatgaac cggtccatga cgaaagcgga gcgggccagg
     6061 tagccgacat cctctaaaag cgccgtaaag ccgaaaaaga gaataaggat gggaagaaac
     6121 gtcaacagcg tgccgacgcc gccgaggatt ccctcgacaa gggcgccccg aatccaccac
     6181 ggggaggagg cggtccagcc gccgactaac ccttgaagac cttggatgac ggatttttcc
     6241 aggaatccct ggagagggat tccgagcgcg tatgtcaggg aaaaaacggc ggccatgacc
     6301 gcggcaaacg cgagaaggcc ccagaacgga tgggtcagcc agcggtcgag ccggtgggtc
     6361 agcgagatcg gcccggtctg gggccggacg agagcggccc gggtcatccg ctcgatccag
     6421 gcgtatcgtc cggaagcgac ggcgaccagg gcgtcgtcat gccgtttcaa taaggcgagg
     6481 atttcctccc accgcccggg aggcacggcc gcggcgactt tccggctgat gtcctcgtcc
     6541 ccttccagga tcttcaaggc gacccagtcg cggggatagg gttcgaggtc cgttccctcg
     6601 agcaaacgtc cggtccggtc caggaccagg cgatggtcct cccggattac cggtcgctga
     6661 ggctcgggaa tccgcccgcc ctcagcggcc gcggcgatcg ccttgaccag ttccttgatc
     6721 ccgccccttg aggaggcctc catgggaacg accggaaggc cgagggccgc cgacagcacg
     6781 ccgggctcga tcgccaggcc ttccttccgg gccacatcca tcatgttgag accgatgacc
     6841 acgggaaccc gcaggctgag gagttcggcg acaagataga gaccgtgctc cagaagggcg
     6901 gcgttgagga cggcgacgac gaggtcggga cgttcccgga tgatgaattc ccgggcgatc
     6961 agttcctcgg gcgaggccgc ggtcaggctg tacgtgccgg gcaggtcgat cacccggaag
     7021 gaggtccggc cgtatggaaa ggttccttcc ttgcgctcga cggttttgcc cgcccagttc
     7081 ccgacgtgct ggtgcaggcc ggtcaagagg ttgaagacgg tcgacttgcc gacattcggc
     7141 gggccggcca gggcgacttt gatttcggcg ggaccagaga agctcatgcg gggagccgcg
     7201 cgggagcgat aaggatccgg tgcgcctctc cccgtcctaa ggcgacgcgg gcctcacgga
     7261 tctggacgat cagcgctccc cggccgggat tttgaaccat gacgacttcg actcccggaa
     7321 cgaagcccag ggataccatc cgggccagca gccttcggcc gccgtccaat ccggcgacga
     7381 cggcccgccg cccggagctc aattgagcca aggtcatcgg gccggcggcc cgcggcgtca
     7441 tggtcggcct cccgtcggcc ggcagcttcg gcagaaaccg ccggcataaa catggaattc
     7501 cttgaggtcc gcgttcagcg tccgcttgat ttccgtcttc aggcggttga ccaggggatt
     7561 ctcgcactcg atgacctgac cgcaggcgag gcaggagaag tgcacgtggg cggcccggcg
     7621 agacgcctcg aaatgcccgt gagattgtcc cagccgatgc tcctcgatga atccttcttt
     7681 tttcaataag gccagggttc gataaacggt ggacagaccg atgcggggat caattttttt
     7741 cgcttccaaa aacagggttt ccgcgtcggg atggccggga attttcctca acaggtcgag
     7801 gatcacctct cgcttcgagg tgatgcgccg gcccgaggct ttcagggcgc tcttgatggc
     7861 cgggattgaa ttcacgtcag gattctttat taagaatgat tctcattatc aattatatcc
     7921 gaaaaaatcc ccttgtcaag gaaaccggat gtcctcaaca gcctgattcg gggcgggacg
     7981 gcccccctcc ggcgaaatat cccgccgccg ggacggcgtc ctcctcgtga cgattttcct
     8041 cgctcggccg tggacgcaag gagccggcga tatccggctc ccagacgaga gcgaacggcg
     8101 gggtttggag atcctttcag ccgtcactcc aggccggtgg gggcgttgtt caggagaagg
     8161 tcacgagttc cggcccggcg ccccttgccg ggagaaggcc gaaacagccg tctccgaagt
     8221 catcattgat ttattcggag aggcaacgga agagcctgcc tggacgtagg aaggatgtag
     8281 atgttaaaaa attagcattt cctcttgaca ggcgggaata agggtgctaa taatttagca
     8341 tgctaatata ttagcatatc gagccgaaag aacggggagg cccgcatgac gaatgaccga
     8401 agcctgagcc ggcgggaacg ccaaatcatg gacgtcatct acgagctgaa ggaggcgacg
     8461 gttcttcagg tccttgagcg cctgccggat ccccccggtt attcggcggt ccgggcgctg
     8521 atacgagtcc tggagcggaa gggtcacctg actcaccgcc gggacggacc ccgttatgtt
     8581 tattcccctc tcgtggccaa ggaaaaagcc cgtcgaaacg ccctccgcca cgtgataagg
     8641 acgtttttcg acggctccac ggaaagcgtc atggccgccc ttctcgacat ttccgagaag
     8701 aatctgtccg aggacgatta tgttcgtctc cgggagctca tcgacaaagc ccgccgggag
     8761 ggtcgctgaa atgcagggaa tcacggctac ggcggcgctc ctggtcgaag gctttctcaa
     8821 gacaaccctc gtccttgtcc ccgccttgct gggggcggcg gcctgtcgga ggcgcccggc
     8881 ttctttccgc catttccttc taagcttcgc tctcatcggc ttgctgtttt tacccctttt
     8941 ccgtcttctg ccgggaggat ggaagacacc gcttcttccg gctcggccgg ccgcggccgt
     9001 gtcgtcttcc gccgtttttt cttccccgcc gccggcggcg ctttccggcg cttccgcggc
     9061 ggtttccttc ctttccgtcc gtgacggttc gccgtcttcc cgaagcggag aatccgcagc
     9121 cggcccgagc aacgccccga ccggcgggcg ggcggttccc gcggcggtct ccggcgctgc
     9181 tggcggattt tttcccgctc atgcggccgt ttttgcctgg ttcgccggat tcctcttcct
     9241 gctcttgaag ctggcgttcg gcctcgtcgg cgcctatcgt ttaacccggg aaggatggga
     9301 gccgggagac cgaggctggc ggattctgat cgcccgtttt ctggcggtgg ttccgctgag
     9361 gaggggcgtc cgcttgaaaa gtcacccgga agtcgccgtc cccctgacct ggggctggag
     9421 aaagcccgtc gtccttatcc ccgtcggggc cgacgcttgg gaggaagcgg agcgctcggc
     9481 cgccctgttc catgagcttt cccacatcaa acgggcggat ttcctctttc tgctccttgt
     9541 ccggctcagc ctggcggtgt tttggtggaa tccgctctgc tggctggcgt acgcccgtct
     9601 tcgaaaggaa caggaaatcg cctgcgacga gctggtcctc cgcgcgggga tcaagccgtc
     9661 gatctacgcc gccggccttc tggccttccg ctcgtcggcg gatttccgcg gccgggctgc
     9721 cgcggccggg ccaggccttt tgggcggtcc gttcttcaag gaccggctgg cggcgatttt
     9781 gaggcaaaaa caaacgttca aggaggtcaa gatgaaatcg agaatcatgc tggcggcttt
     9841 ggtcgtccta gccatcgcct tcatcgggac ggcccgcccg gccgtcgcgg gcaacgcggc
     9901 gtcatcttcg ggcgctcccg tcgacgaaac gttgtccgcc gcggctccgg ccgccgccca
     9961 ggaggtgaaa gcggagaagg aaaccatcaa ggaaaaggaa aaagagaaga ccgcgctgaa
    10021 agagaaagag aaaaagaaag agaaggagaa gacctctgtc aagacgatca tggtgacggc
    10081 cggcggcgac aaggaccagc cgattgaaat caccgtcacg ggagaaggcc aaattaaaaa
    10141 actgcttctg gacaagcccc tgaccctcaa aaccggcgag gacggccgga tcacgttcag
    10201 cgtggaggga aaagaggtca cggtcccgaa gggcgggacg atcagcatcc ggggcgccgg
    10261 cggcgcgctg cgcttcatca aggaaggccg gatcgtgcag ctcgaagagg gagaggccgc
    10321 cgacttggcc ggggacggcg acaagatcgt ggtgatcgaa aagcccgggg aagggggatc
    10381 gaagcatacg atcgtcaagg aaatccggat caagaaggga gaggccggcc gcgcgatcga
    10441 gatcggcaag gaaatcaaag ccggggagca gcccgtcaag gtgatcatcg aggagcacgg
    10501 aaaaggaaag cccggcgtgg tctggacgac ggccggagag ggtccggcgg ccctggcggt
    10561 gatcggcgag cgggatcttc ttgaaaaggt caaggcgatc cgggagaaag ccgaggcggt
    10621 caaagccaag acgctggaat tcgaggagct cgaaaaatcc ctgaagaacc ttgaagccga
    10681 gcttaaggac gccgaggacc ggctcaagaa catcacggtc cggatcgatc aaggcgagga
    10741 cgtccggatc gtccgggagg acgaggccgg gaagaaatcc ttcgtctgga tcgaggcggg
    10801 cgacgcgggg aaggacggcg aaccgatgaa agtcgtctgc cggcgcgatg cgggaacgct
    10861 cgaaatcacg ctgacgggga tgaaaggacc ggagggccgg tcggtgcatg accgtgccct
    10921 ggcccggata aagagagagc tccccaagga agccatgctt ctcgactcga atttcgatga
    10981 gggcgggggc aaaatgacgt tcaagctcga agtccccgcg gaaaccgcga cggacaagga
    11041 cttcgtcgac acgatcatcg acatcgtttc ggaagaaacg aagaaataga cggctccgga
    11101 caagggcgtc cctcccggac gggcgttcgg tccgatcgga aaggcctttc cgggattgca
    11161 ttcggcctcg gattcgggga tgataggccg agtccggaga aggagggacg ccgtggattt
    11221 aacgaaaatc atccgaaaag aaaaccttgt ctactcgggg aagtccaagg acatctaccg
    11281 gataccggga ggaccctgga agggcaagta tgccttcgtc ttcaccgatc ggggcacggg
    11341 gtacctggac aaggccggac ggccggtctt cgatcccggc tacgactccg tggtcggcga
    11401 aatcccgggg aagggagcca tcgccgggcg gttcgcgacc cgtttcttca acctgacggc
    11461 cagtaagaga atccccagcc acttcatcgc ctcggttcgg gacgaggtca tgatcgtcga
    11521 acccgccgtt cccctcggcc tgccggaaca agccccggaa ttcgagggat ccgccccgct
    11581 tctgaacctg gaatggacct ggcggaacaa cgctaccggg agcttctggc ggagataccc
    11641 gttcgtccgg ccgtgccagg gcctggaccg ggtcgtcgag gcctggacca agggcgattc
    11701 ggacaccctc atcaccttcg agtcgctggt cgcggccgga gtgatgaccc gcaaggaggt
    11761 cgtctggacg gaggccttcg tcaagcggat cgcggcggtc gtcacggagg agatggcgtc
    11821 ccgggggctt catctcatcg acgggaagat cgagctcggc cggttgaagg gcggcgacgg
    11881 gagaatcgtc ctcattgacg agatctcccc ggacgtcctc cgcgtctgca aggggtacgc
    11941 cccggacggg aagaacggct gccggaaaag ccggacatgc atccggacaa gccaggcggg
    12001 gaacgtccgc aagatctcgg ggaaaaacgt cctgaccgcc gcccagctgg aggaggtctt
    12061 ccgaaccgcg ggctaaagga tgaagccgac gcccagggtc agggtcagaa cgtcggtccg
    12121 caggagtcgg ccgaattccg gccccatggg ttcggtccgc aggtcgttaa tgtcccggcc
    12181 ctttccctcg ccgaaggcgt agagaaaccc gatccagcgg tacgtgacgg ccgcgttgag
    12241 aaagacagag ggccccaggt cgaggtgaag acctgcgccg gcctggagcc ccgccccgac
    12301 gtaggaggcg ttcaaggtct tgcctcggta gaacgaaccg cccttgaccc taaggaacgg
    12361 gagggtgatc ccgccgagga aatacggccg gagaggaagc ttcttcagaa aaaaggacct
    12421 cccgttgaga tcgagcgcct cgtaggtcgc ggcgcgggat ccgttttcaa gggcgacggt
    12481 ccggcggaga cggaggaagg aaacgtccca ggacccgttg taggatttcc tcccgtagcc
    12541 gacggaccaa gccccgcccc gttccagccg cggaacgtaa aacgcttttt caaaatgcca
    12601 gagcgtcagg ttccggttga gatcgcggtt gaagtcgaga aattgcccgg agagcaggag
    12661 aaacgggccg tcgcgtcccg ggccggccgc cgaggcggcc gagacgaagg ccaggaggag
    12721 cacggcggtc agcgcgcggc gggccatggg agacaatgta gaccggaggc cggcgttagt
    12781 caacacggcc ggcggatcga cgggaacgtc caggacgatg gatcggcttc gttcctcttg
    12841 gaacctcgac gggcggtttg tctttgcctt cttccgggga aaccgaccat tcccgcggcg
    12901 gttgacctcc gcatcgtctt ttcccctata atgtccgggg agaacaacga tgcgccaata
    12961 tgaaaccgac attctgatca tcggggccgg gccggccggc ctgacggcgg ccatctatgc
    13021 cgcccgctcc ggcaagaaaa cgctggttct ggaaagcgag cgggcggcct cgcggctcgc
    13081 cctgggatac gagctcgaga attatcccgg gttcgccgcg atcaacagcc gcgagcttct
    13141 ggataagttc cggtcgcagg cggccggttt cggggcggaa ttcgcgacgg gcgatgtcat
    13201 cgccctgtcg ctggagtcca gcccgaaatt cgtctcgacg tccgacgcct ttatcgaggc
    13261 ccgggccgtc atcctggcca ccggcaagcc gttcgccaag gagcggcaga tccccggaga
    13321 ggaacggctc ctcggctacg gcgtgagcta ctgcgcggtt tgcgacggcc ctctctataa
    13381 agggcgcgac gcggccgcct acggccattc ggccgaggcg atcgaggata tctggatcct
    13441 ggatcagacg ggcgtccggg ttcattggat ccccgggcgg gtcaaggatc ccgccgccct
    13501 ggaggaagcg ttcctcaagg ccgagaagcg cggcatcccg gtccacggcg gggccgagat
    13561 caaggagatc gccggagaga agaacgtcga acgcgtcgtc ttgaaaagcg agggcggcga
    13621 acggacgctg gacgtcgagg ccgtcttcat ttttcgggaa gtcccgaccg gcccgctctt
    13681 cgccaagtcc ggcctggccc tcgaccataa gcaatgcctg gccgtggacc gcttccagag
    13741 gacgaacctc ccgggcgtct tcgccgccgg cgatctcacc tgcggcggcc ttcaagtcgt
    13801 ctcggccgcc ggcgaaggct gcgtcgccgc cctccaggcc ctggcttatt tgcgcaagta
    13861 gccctatttg cccttcatca gctcccggac ggccgcctcg atctgctggt cgcgcccggc
    13921 cgacatgacg tccggatcgt tcttgacccg gatgtcaggc tccagctggt tgttctcgca
    13981 aagcttcccg tcgggcgtcc gccatccgcc ctgggggatc ccgaagcgga gagtcggatc
    14041 gatctgggtt tcccaccaga cgaacgttcc cgtgcccggc acgggcatgc ccagcgtttg
    14101 cccgatcccc ttgatcttgt attccacggg gaaaaggtgg gcgtccgagt aattggcttc
    14161 gcccatcagg acgatcgacg gcttgatcca cttgttgtac ggctcgtagc cgacgagctg
    14221 gccgcgaggc acgatgtcga agacctttcg gccgttcaag aaatccgcga gctggtcgtg
    14281 gaggctgccg ccgccgttga agcgggtgtc gacgatcagg gcgtccttct ccatgctgag
    14341 gccgaagact tcatcgatga ccgtccggaa gctctgatcg ttcatgcccc ggacatggac
    14401 gtagccgatc cggccgcccg aaagccggtc cgtctcggcc cgccgggcca ggacccagcg
    14461 ccggtagaga agggcggcct cttcctccaa ggtgacgggc ctgacggcct cctcccagcg
    14521 cgaggacgcc gccggatcga gaacggaaag cagggtgagc tggccggcct tccggttgag
    14581 cagggcgtaa tggtcgatcg tgccgtccag ggtccggccg tcgatttttt cgatgatgtg
    14641 gcccgcccgg atcttgaggc tcgccttgtc cagcggcccg ccgctcaaga cctcggcgac
    14701 tttcaggccg gggccggcgt aatcataatc caggaacagg ccgagcgaag ccgtggcgtc
    14761 ggccgacggc gtccgggccg ggttgtaata acagccggta tgggaggcgt tcagctcgcc
    14821 cagcatctcg ctgaccatct cggcgtaatc atggttgttg ttgatgaagg ggaggaattt
    14881 gcggtagatg gggtaaaagg aatcccagtc cgccccgtag agatcctcca ccaggaactt
    14941 ctgcttgatc tgccgccaca tgtggtcgaa catgtaagct ttctcggcgg cctcgtccag
    15001 gcgcatctcg ccggtgatgg ccaagggctc ctgccggccg ctgtccggat cgaccttgag
    15061 gatccgtccc tcggctccga tgaagagcgt cttgccgtcg ggcgacagct ccatcgaggg
    15121 cctctggacg cccagcttgg cgagcagctt ggtgtcgcgc gtccgcggct cggtcaccca
    15181 taaatcataa ccgcgttcga accgggccag atagaagagc ttctcgccgt ccttgctcaa
    15241 gacggcgtcg ctgatgtccg ccgaatgaat ggagaggcgg accttccgcc ggtcgagccc
    15301 gtcgagctcg aacgccagtt ccttcttcgg ttcttccttt ttaaggccgg ccgccttggc
    15361 cttggcgtcc gcggcctttt ctttctccgt tttctccttt tcgtccttgg ctttttgctc
    15421 cgtttccttc agcaaagcga attcggcctt ggagaggagg aaccggtcgt acgcctcccg
    15481 ggtgaaaaac atcccgtaga tatcgtaggt catgggattg ccgtccgtgt ttgtcaagcc
    15541 ctcccgggtc gagccgtagt acatcatcgt cccgtccagg ccccagcgga cgccgacgtt
    15601 gtcgaatccg ctccgggtca ggttgatgac ccggccccgg ccgtcgctcg agacgaggcc
    15661 gatctggggg gtgaagaggc ggacgacgcc gaattggacc agaaaccact tcccgtccgg
    15721 gctccagcgg tagctctggt cgccgtcggc gtaggagtaa ttgtacgttc ccggcagaat
    15781 cagccggctt tgcttggtcg ccaggttgat gaccttcaag gcggtccggt tttccaggta
    15841 agcgacttcc ttgccgtcag gggagaattc gggctgaaac tcctcggccg ccgtggccgc
    15901 gaccggttcc tccttgagga gcgtcgcggc gaagaaatag ggctcctcct tgcggacgat
    15961 cgaggtcgcg tagacgttcc agttgttgtc tcgttcggcg gcgtagatga gtgtccggcc
    16021 gtccgggctg aagctcacca tccgctcctg ctcgggggtg tcggtcacgc gcttggccgg
    16081 cccgccttcg gcgctgacga cgaaaatctc gccccggaag acaagggcga tttctttgcc
    16141 gttgggggat aggcgcatct cggtgagccc gctccccccg acgggcagaa cccgcgggag
    16201 gacggcccgg ccgtcctggg cgatgcggac ggcgagcttt tggggagccg cgtccccggc
    16261 ttgggtgtag atttccccgt cgtagccgaa gcacaggacg ccggtatccg agcgggtcag
    16321 gaaccggacc ggattcttgg cgaacgaagt cacggcggtc gcggaggcgg gacggctcag
    16381 ggagctcttg tagatgttga acgtcccgcc ccgctcgctg agaaagtaga agtcgtcgcc
    16441 gttggggtcg aagacgggat tccggtcctc gccttcatta tccgtcacct ggatgttctt
    16501 cttggccttc aagtcataca cccagatatc gcgcgtcacc gcggacgtgt gatgcttgcg
    16561 ccagacgttc tcacggccct tggcgtcgtg gtagaggatt ttgtccccgg cccggttgga
    16621 gacggccgcg accgccggct gggtcaggac aagggacgct tccccgccgt cgacggacac
    16681 gctgtagagc tcgggaaagc cggtcatcgg gaactggacg ttggctgccg tgtcctggcg
    16741 ggccgaggcg aagaggatcc gccgtccgtc ggccgtgaag ccgctgggga tgtcggccgc
    16801 ggaatggaag gtcaggcgcc gggcttcgcc gccgccggcc ggcatgaggt agatgtcgaa
    16861 attgccggag cggtcggagg caaaggcgat ccaccggccg tcatggctcc agaccgggga
    16921 ataatcgtag gcttcgccca cggtcagggg aacggccgtc ccgccggaag acgggatttt
    16981 gaaaatgtcg cccttggcgc agaaaaggat ggcttggccg tccggcgaga tggccgggta
    17041 ccgcaaccac aagggattgt cctgggaggc ggcgggagcc gcgcccagaa gggaggcggc
    17101 gagcacggcc agggcggccc agggcgtcgc ggaccgatgg acgggacgcg gccggcggaa
    17161 acggagggaa gggaacggac gaagcatggg gattctcctt gatgaaggca tctcattatc
    17221 atacgccatt caataaagaa aagttggcaa cagccgccgg gccgtgttat cttccggggg
    17281 acaaggaggc ggtcatggcc atcatcatcc gcggcggatc ggtcttttcc cccggggcgg
    17341 acggcgcccg cgacattctc gtcgccggcg gccggatcga ggcgatcgcc ggcccggaga
    17401 cggtcgtttg ccggggattc gagacgacgg tgatcgaagc cggcgggaaa agcgtcctgc
    17461 ccgggttcat tgatccgcac gtccatatcc tgggcggcgg aggggagggc ggtcccgcga
    17521 cccgggctcc cgaaatcgcc gttgaggata tcgtcgccgg cggcataacg accgttctcg
    17581 gctgtctggg cacggactcg gtgacccggc atatgagctc gctcctggcc aaggcccggg
    17641 ccttggagat cgaggggatc tcgacgcgat gcttctgcgg ctcctacgag atcccggtca
    17701 agaccgtcac cggcagcgtc cgcggagatc tcgtcctgat cgacaggatc atcggggccg
    17761 gggagatcgc cctgtccgac caccgctcct cccagccctc gttcgaggaa ttcgcccggg
    17821 cggccgccga atgccgggtc ggcgggatgc tgggcgggaa ggccgggatt ctccacgtcc
    17881 acatgggaga cggagcccgc ggcctggaat acctgcggcg gctcgtccgc gaaaccgaga
    17941 ttccggtgac ccaggtcgtc ccgacgcact gcaaccggag ccggcggctt ctggaggagg
    18001 cggtcgaata cgccaagtcc ggggggcgga tcgacgtgac ggccgggacc gatccggacc
    18061 gagaatccga ccatgacgtg agcgtcgaaa cggccgtccg gctgcttata aacggcggcg
    18121 tgccgctgga gcgggtgacc gtcagctcgg acggcaacgg aagcctgccg gttttcgacg
    18181 cagccgggaa tctcaccggt ttgaccgtgg cttcgccgcg ggcgctcctc caaacgttcc
    18241 gttcgcttgt cggccggaag atcctgactc tggagcaggc ggcccggctc ttttccgcca
    18301 acccggcgtc gatttaccgt ctggaggaaa agggggcggt cgccccgggg cgagacgcgg
    18361 acctcctggt tttcgacaag gagctgcaac tgacggacgt gatcgcccgc ggccgggttt
    18421 tgatgaagga cggaaagctc ctggtcaagg gcgcgttccg ctgaatcggc ggttcgggat
    18481 tggaatctgg cgagggagac cagggagctc acgacggccg gagcagcggg gcgggccttg
    18541 cgggaggctt ttttttcgtc ctcaagccgg gccgtacttg aacagcaccg gcggaatcag
    18601 cgtcagcaga ccgaaaatca cccaggcgat caccaggggg atgagccact tggcccattt
    18661 ctcgtagcgc agcccggcca gcccgagaac ccccatcgtc actcccgagg tcggcaggat
    18721 gggattgatg tactcggcca gctggaaggc gaagacgctc gtctgccggg tgatcccgac
    18781 caggtcgccc aggggagcca tgaccggaat ggtcagggcc gcctgggccg tccccgagtg
    18841 gatgaagaaa ttgatcccgc actgggacag gaacatcagc tgggccgaca ggaccgggtg
    18901 aaggttggaa atcgccgagg ccatcccggc caggatcgtg tccatgatcc ggccgtccgt
    18961 ggcgacgatg aggatggccc gcgtcgtccc gatgatcagg gcggcgttga ccatgtcctt
    19021 ggctccgtcg acgaaggcct tgccgaactc gtctcccgtg agacggccca agatcccgga
    19081 gaggatgccc atggccagga atatcccggc gatctcgttg atgaaccacc gatacttgag
    19141 aatgccgaaa atcatgaccc cgaatccggc ggcgaacact ccaaggacca gcttgtgccg
    19201 caagagaacg gggagttttt ccatggccgg cgtcgtcccc aggagctttt ttcttttttc
    19261 gaggtcgtcc gcgaagaccg ggctgatctc gggcttcttg aggacgcggg cggcgtagcg
    19321 catgacgagg acgacggcga tggccgtgcc tcccgcccaa atgatcagcc ggtagcccat
    19381 ccccgaataa agaggcaggc cggcgatccc ctgggcgatg cccacggtga agggattgaa
    19441 gaaggcgccg gcgaagccgg cggccgcccc gagaaacggg atcgagacgc cgacgatggt
    19501 gtcgtagccc agggaaaggg ccagggggac gaagatcagg atgaagggca tggtttcctc
    19561 gcacatgccg aagaccgccc cgccgaggga gaacaccgtc atcgtgaccg gaatcaagag
    19621 aacccggagg gccctgctcc gcccgaagag gcaggcgatg ttgtggatga cgaccgtgat
    19681 cgccccggtt ttctggatga ccgagaacgc cccgccgacg atgaggagga tcccgatgat
    19741 cgccgcggcg tcgatgaatc cccgggcggg agaaacgaag agtccgccca agccctgggg
    19801 ccggctctcg atccgctggt aggaaccggc gacgggaacc aggcgcccgt ctttttccac
    19861 ccgctgatat tcgcctccgg ggatgatcca ggtcgcggcc acggtcagga cgaccatggc
    19921 gtagacgagg atgagcgtgt gggggattct cggttttttc acttccggcc tccctggacc
    19981 gccccgatcg ccggatcgta gacgtcgccg ggaaggagga tatggatggt catccccgcc
    20041 gcgcccagga gcccgtcggc cgtccgggag ccggaagccc ggcgggcgtc gtagacgacg
    20101 gcttggcgga ggccggcgat ttcgtacttg ccgtcgggac ggacccagac ggccgtcgat
    20161 tcgtcgattc cgacgccgag aagcccggga ttctccagga cgacgctcat cagccggttg
    20221 tgccggcggc gggtcacgaa gtgctggtcg atgacggcgc tggtgatgaa tccgaatccg
    20281 cggacggtct cgacattgcc cgcctcgatc gtccgtccgt tctcgtcctc ctcccgggtt
    20341 ctcttttcgt tcccggtgat catcatttcg ctcatcgccg cggccccggc gctggtcccg
    20401 gccacgacga gcccccggcc gtaaagctcg agcagccttt ggtggatcgg cgtgtcgagg
    20461 agaacggccg tcaggcggga ttggtctccg ccgcagaaat aaacgccccc cgctccttcg
    20521 agaatagcag cgctttccgg ccggagcgcc tcctcccggg tcagatggaa ccaggaggcg
    20581 tcgggcgctc cggcggcttt cacctcggcc gccgtttccg ggccggactc ctccggcacg
    20641 ccgctggcca tggtgaagat gacgattttc cccgcgccga atccccgcga cagggccacg
    20701 aatttttcag tcaggccgtc cggccggtcg ccgcccccga tgatcagcag ggaccctttg
    20761 tccgcggccg gaagaacgaa ggaaacaagg agccctaaga caagggcaaa gacgatcttt
    20821 ttcaccggat gcctctctct cggatgcgcg ggtgaccgcg cggccgaaag catcctatct
    20881 tcaggccgga cgaagtgtca agaacggccg ggctcgaaat ccgggccggc cgcgggaggg
    20941 tcgtcaagcc ggcggatcgg agacgccgga cacggtgatc tccgtccgga tgtcgacgct
    21001 ctttttcagc atggcgaaga cggggcaata cttgtcctcg gccagctgaa gggctttgtc
    21061 cacggccgcg ggatcgaggc ccgggccgga cacgaggaca tccagccgga tcgaggtgaa
    21121 gacgcgggga tgctcggctt gcttgaggcc cttggccgtg agggaaaacc cgcggacgtc
    21181 ctggcgcatc ttccggagca gggagacgat gatctgcccg gcgcacgaag ccaagctgat
    21241 cagcagcagc tccatgggct tcgctccgtg gtcgtcgccc tcgggcgggg ggacgtcgat
    21301 ggcgatgggc tggccgaaat ccgttcggcc ctcgaattcc agttttccct tccagatgac
    21361 gcgggcggcc agctcttcag ccatgctcat gtcctttcca aagtgacgct cggcggatcg
    21421 cccgtccatt ataacgattg ggtgccgctt ggctcaactt tcctccggcc ccatcaggcc
    21481 ctggctgtct gacttccggt gaacggcgcc gccggaaaaa tcaatccccc aagccccggg
    21541 aggcgtcccc catgccgtga cccgggacac aggctggcct ggggatcgtt ttttccgtac
    21601 aatccattcc caacccatga tggtccggtt taaagactcc accgcggacg agctgcacgc
    21661 cgccctggcg ccccggggca tttccctgcg ccttgcccga cggcttcaag cggccgtgct
    21721 gaggcgcgac gccttccctc ggagcctgcc ggaagtctcg gaaaaaactc tggcctgggt
    21781 gagggagaac gccggcctgc cgaagctcgt tcaaaaaggc aaagcggttt cagcgcggga
    21841 cggattcgcc aaatatctct tccaaggcga aggtcccgaa cccttcgaag cggtccggat
    21901 cccgctgctt catcgggccg gagaagagaa ggttgtcgcc tgcctgagct cgcaggtggg
    21961 atgcgggctg ggctgcgcct tctgcgcgac agggcggatg ggccttgtcc gcaacctctc
    22021 ggcctgggag atggttgatc aggtcatccg ccttgccgcc gattcggaac atcccgtccg
    22081 cggcgcggtg ttcatgggga tgggcgagcc gatgttgaac tatgaggcgg tggtgcgcgc
    22141 ggcccggatc ctgaacgagc cgtgcgggct ggccatttcg gccgaggcca tcagcatctc
    22201 gacggccggg atcgtgccgg gcatccggag attcacggcc gaccggttgc cgttccggtt
    22261 ggtcgtatcg ctgaccagcg ccgatccgtt ccggcggcgc ggcgtgatgc ccttggagcg
    22321 gacccatccc ctgccggagc ttatggaggc gcttcgggaa taccacaaag cgtccggaca
    22381 gcgcgtgatc ctggcttgga cgatgatctc ggggttcaac acgcgggaag aagatgccat
    22441 ccagctggcc gctctggtcc gggggttgcc ggtcaggctt gatttgatcg atgtgaacga
    22501 cgccgcgggc cgcttcaagc ccccgtcgcc tccggagctt gccgcgttcc gggatgcgct
    22561 gagaaagcat ctgaaaatgc cggtggccag acgctacagc gggggaaggg acatccacgc
    22621 ggcctgcggg atgttggctg gctcgcttcc cggcccttcc aggcgggaaa gccgctcgcc
    22681 tgcttcataa aaatccgttt tcgccgggga gggaatccca agccggccgt cgcttgatca
    22741 agccggcggc atcgagcttc cgccgcttgt cgaacatcgt gaccgccgcg atccgggtcc
    22801 gagaaaaagg gattcaagga cggctttctt gatgacctcg gcgcatcatt gtcgcggctt
    22861 cattcggcac gacggaaaat tcatcaagtt gttccctttc ccgattcccg agccgatcat
    22921 caagcctctt ctctgtggcg ttacatctcg atgtccgtat ggctatcaat ttagatatgt
    22981 gatatatttt atttaacgcg atgatttcat taatcggagg tcccgtgaaa aaatcccgtc
    23041 gaacctttgt ctgcgttttt ctcgccttga cgctccttct tccggttttt tcgtccgccc
    23101 aaggcacccg ggcggattac gaacgagccg gcggcttgcc ggagaaatgg aaaaacctgg
    23161 ccgtcaacca gcccgaccag ccggaatgga tcgaaaagac gcataagttc tggtaccgga
    23221 aatcgaccgc cgctggatat gaattcgtct tggtcgacgc ggacgccgcg acgaaaacgg
    23281 ccgctttcga tcatgaaaaa ctcgccgggg ctttgaccga ggctctcaag gaccggatcg
    23341 atccgaaaaa tcttccgttc acccggattg aatacgtcga tcaaatgaag gcgatcgagt
    23401 tcgaaagcgg ggaatcccga tggaaatttg atctggccgc ggggaccgtc gtcagaatca
    23461 gccgggttct ctcccgatgg agcgacgaaa gcaactggca ggatgtcggc ggacgggccg
    23521 agccggccgc ctcggccgaa gcgaaggcgt ctcccgacgg gaaatgggag gcgttcgtca
    23581 ggaattacaa cgtttacctc cgtccggcgg gcggggctcc ggggggcggt ccgcagaccg
    23641 ccggcttcgc cctcagtctc gacgggatgg agagcgatta ctacagcttc cgatcgatca
    23701 cctggtcgcc ggattcaagg aacatcgccg ctttccgcgt ccggcggggg cctcaccggc
    23761 ggatcgattt catcgaatcc tccccggacg gacagcttca gccgaaaacc ttttcgctcg
    23821 ggtacaccaa gccgggcgac gtgatcgatt ttctccgccc ggtgctcttc catgtggacg
    23881 agcgaaaagc cgtggacatc gacaatgctc tcttcgccaa tccctacgat ctttccgagc
    23941 tccagtggag ggaggacggc cgggccttca cgtttgaata caaccagagg ggccaccaag
    24001 tctaccggat catcgaggtg gacgcggcga ccggccggcc ccgggcggtc atcagcgagg
    24061 aaccccgcac gtttttcccc tacttggaaa tctccagcta cgggaaaaaa ttccggtacg
    24121 acgccgccga cggccgggaa atcatttgga tgtccgagcg cgacggttgg aaccacctgt
    24181 atctcttcga cggagcgacc ggccaagtca agaaccaaat cacgaaaggc gaatgggtcg
    24241 tccgcggcgt ggacaaagtg gatgagacgg cccggaagat ctggttcaag gcgagcggaa
    24301 tgaatccggg ccaagaccct tatttcactc atcattactc gatcaacttc gacgggacgg
    24361 gcttgaccgc cctgacggac ggcgacctga cccaccaggc cgcgttttcc tcggacatgg
    24421 cgtttttcac cgacctctct tcgcgtcatg accgggcccc gatcctggac gtccgccgga
    24481 cggccgaccg cggcctcgtt ctccgcgtcg agcaaaccga catctccggt cttgtcaagg
    24541 ccggctggaa ggcgcctgaa gccttcaggg ccaagggccg cgacggccgg acggatattt
    24601 ggggcgtcat ttaccggccg ttgaatttca gctccaagaa aaaatacccg gtgatcgaat
    24661 acatctacgc cgggccgcac gattcccacg tccccaagac attcgtcggc ttcagtcccc
    24721 tgcaggccct ggccgagctc gggttcatcg tcacccaagt ggacgggatg gggacctcca
    24781 accggtccaa ggcgttccat gattattgct ggaagaactt ggccgacgcc ggctttcccg
    24841 accggattct ctggcacaag gccgtggccg ccgaataccg gtattacgac atcacccgcg
    24901 tcgggatcta cggctggtcg gccgggggac agaacgccct cgccgccctg ctttttcacc
    24961 ccgagttcta caaggcggcc ttttcgtcct gcggctgcca cgacaaccgg atggacaagc
    25021 tctggtggaa cgagcagtgg atgggctggc cgatcggccc cgaatacgcc gcttcgtcca
    25081 acgcggaaaa cgcctggcgg ctccaaggca agctgctcct gttggtcggg gagatcgata
    25141 cgaacgtcga tccgtccagc acgatgcaag tcgccgacgc cctcatcaag gccggcaagg
    25201 aattcgagct ggtcgtcctc cccggcgtga atcataccag cggagggacg tacggagacc
    25261 ggaagcggaa cgatttcttc gtccgcaacc tcctcggcct cgagccgccg gactggaacg
    25321 cgccggggcc gccggcgaat aagtagcctt actttttcca gaccggcacc ggccggccgg
    25381 gcgtccaggg cgccccggcg gcctccaagg cctcgcccag ccggcgcagg tctccgtcga
    25441 tcaggccgcg gagcttttcc aggaccgccc cgaaggcgtc ggccgcgacg gcgtaatcct
    25501 ttttcacggt ttcggtgagg ggcccggtcg agccgagctg ccgcgagacg cggtccagga
    25561 gcgacggcgt ccgggcctcg gaccggcgca tgagggtgac gtcgccgtgg aggtcacgga
    25621 gggcctcccg gatcccggtt tcgatcgccc gggcggtttc cgcgagcttg ggatcggccg
    25681 ccggcgtgtc gagcagcgct ttccggacgt aggccaggct ccgcagggcg ctgtcggcgg
    25741 cttcgccggc tcccatcatc gcccgctgca gcccgcccgc cttttcctgg aaggccagga
    25801 gggcggcttt gtccttttcg gcgagggagg tcagcgtcag cgattcgacg gtgaacgtct
    25861 ccgggccggc gagcggaatc aagacgccgt cgattttttg ggccagggaa acagagaacg
    25921 ttcccgggac aacgagcggg cccatcggct ccatgtccca gggatcgcgg accgcggact
    25981 ccagccgggt cggctcgacg gccggatagc gaaggtccca agcgatccgg tggaggcccg
    26041 gcccggccgg tccggtcagc cgccggacga ccgatccgtc cgcggccgcg acggtcagga
    26101 tgatctcggg cttttcctcg tcctcctcct tgcggagaac gtcccagccg gggaaagaca
    26161 cgggttttcc ttccttcaga aacttggctt cctcgtcgcg ccgggcctga gcggccggct
    26221 tcagcccatc cttaagataa taggtgaaaa tcgcgccgaa cggcggattg ggagcggtga
    26281 agaacgtctc tccgagacag cccttgtccg gtgaatcgat cggcctccgc ggaatgtagg
    26341 agagggtctt tttcaccggg aagagcgcgg ccggccgggc gagaagggtt tcgtcgatcc
    26401 ggcgcagcgg ggagaaatcg tccaggacga aaaagccgcg gccgaaagag gcgccgacga
    26461 gatcgccttc ccgctcctgg atctccaggt cccggaaaga gatcgtcggc acgccgccgg
    26521 ccatcttcag ccagcgcttt ccgccgtcca gggtggcgaa gacgccgaat tccgtcccgg
    26581 cgaacagaag atccttcttg acgggatctt gggccacggc ccagacgatc gtccgggccg
    26641 gaagatcgcc cgaaatcgag acccaggtcc gcccgcggtc gtcgctccgg aggagaaagg
    26701 ggcggtaatc gcccgtcttg tgcaagtcga cggcggcgaa gaccgtgtcc cggtcggtcc
    26761 gggaggcctt gatctcgttg acgaagaaat tctccgggac gccgggcagg cgatcgattt
    26821 tccgccagga ttttcctccg tcctcggtga cctggatcag cccgtcgtcc gtcccggcgt
    26881 aaatcaaacc ctccgccagg ggcgactcgc tgagagtcgt gatcgtcgag aagagggaca
    26941 tcgccccgtg gtcccagagg gcgtcgacgc tccaggtttt gcccatgatg ggctgggcga
    27001 ggcggaagat gttccgggtc agatccgggg aaatccgcgt ccaggagtcg ccccggtcgt
    27061 cgctcctcca gacatgctgg ccggcgtagt agagccgggt atgggaatgc gggctgatga
    27121 ggaccggcga atcccagttg aagcggaggg ccggttcgcc cggctcgggc ttgggggaga
    27181 tgaagatcgt ttcgtggctt ttccggtcgt agcggaggag gttgccttcc tgccattcga
    27241 ggtagatcgt atccggatcg acggggtcga tcgcgcaggc gtagccgtcg gccccgaacg
    27301 tgatcgtcca gtcgaagttg gcgatgccgt tggcgttgag ggtgcgggag ggcccgagct
    27361 ggctgccgtt gtcctgggtt ccgccgtgga cgttgtagaa ggggaggtcg ttgtccaagg
    27421 cgagcttata gacttgggtc acgggcaggt tctcgaagaa ccgccatgtc cgcccgccgt
    27481 cgcgggtctc gtaaacgccg ccgtcgctgc cgttgaggag gtaatcggga tcgccgggga
    27541 ggaaggccaa ggcgtggttg tccccgtgct tatttttctc cggaacggtg cggaacgtcc
    27601 ggccgccgtc gtccgtcacc tggagccagt aatccatctg gtaaacgcgg tcgaagacgt
    27661 tggggtcggc gaagatctcc tggtagtaat gggggcccgt gccgccgctg aggtaagaat
    27721 tacgcttctc aaagctttca ccgcggtcgg ccgagcggta gaaaccccgt tcctcgggcg
    27781 aggcctcgat cgtggcgtag acgacgcgcg gatcgagggg cgagacggcc aggccgattt
    27841 tgcccatgtc gcccttgggc aggccgacgg agagcttgcg ccaggttttt cccgcgtcct
    27901 cgctcttgta gatgcccgat tccggcccgc cgcccatgaa ggcggcgacg gaccggcggc
    27961 gctggtaggc ggcggcgtag aggatgtcgg gatcgcccgg ctcgaattca gccgaggcga
    28021 cgcccgtgtc ggccgagatg atcaacgacg gcgtccaggt ccgcccgccg tcgacggact
    28081 tgaaaaggcc gcgctccccg ccgggcgacc aaagcggacc ctcggcggcc gcgtaaacga
    28141 cgtccggatc gcgcggatcg atcaagatcc gggcgatgtg ctcggaattc ttcaagccca
    28201 tgttcgtcca ggtcatcccc ccgttgaggc tcttgtagac gccgtcgccg tagccgacgt
    28261 ggcggccgct gacgttttcc cctgttccaa cccagacgat ctcgggacgg acgggatcga
    28321 gggcgagaca gccgatggaa taggaggact ggccgtcgaa gaccggcgtc caggtcgtcc
    28381 cggcgttttc ggtcttccag acgcctccgg agccggccgc gacgtaccac gtcgcccggc
    28441 gcttcgggtg gatgacgatg tcgctgatcc ggccggacat gacggccggt ccgatattgc
    28501 ggaaggcgag gccggagagg agttcggagg tgagaagagg cgttttgtcc gggacggctt
    28561 tcctggaatc cgcttcggtt ttccggctcc agccggcgag ggagaaaacg aacaggacgg
    28621 aaaggatgag ggcggcggtc tttttcacgg catcctcctg accgggatga aaagaacgag
    28681 gaattaatct agcacaaatc ctatggagac gcagcccctc caggggcggc gcgtcgtccc
    28741 ctcatggatt ttgaagttga gcgaatgcgc ctcttcgaaa aaaaggcgtt cccggccgcg
    28801 ggtccttgac ttctttccgg cggcgtgctg caatgtcaag tcgcggaagc gctttttcgc
    28861 aagccgataa acaaaccggc cggatggatg tccggcccgg agcactccat tgatgaaacg
    28921 acgcgcggtt tccgacggaa aaaagaaaat cctgttccct tttctttcga tcatatcaat
    28981 ccctcaacaa aggagagcgt catgagcgga catggcattt tcgccggcga tatcccccgg
    29041 cgcaaattcc tggagatggg cgtcaagggc ggcctggcga tcgcggcttc gccgattctt
    29101 ctcaaggatc tcttggccaa gacaggaccg ggggatctcc cggccgtcgg accggccctc
    29161 gataaggccg tgctcgaccg ggtcgtggcc cgggccctgg ccaagggcgg ggagttcgcc
    29221 gacgtctacg tcgagcaccg catttcgcgg aacatcctga tggaagaatc caaattccgg
    29281 agcgccgaat tcggcatcag ccagggcgcc ggcgtgcggg tcatcgccgg cgacaagacg
    29341 ggcttcgcct acacggacga gatcggcgag gaggccatgc tgcgggcggc cgaagtcgcc
    29401 tcgttcatcg cccggggcag cgccgcggcg aagccggtcc cgatccgggc gggcgcgccc
    29461 cccgtttaca tcacggtcaa gcttcccttg tccgggatcg ccgacgagaa gcgtctcgac
    29521 gtctgccggc gggccgacca ggccgcccgc ggccacgatc cgcggatcaa aatgacctcg
    29581 atcagctatt acgacgaggt ccgggggcgg acgatcgcca actcggaggg cttgtatctt
    29641 gtcgacgagc tgccgctcct gttcttcatc gtccaagccc tgagcgaggg caacgacacc
    29701 cggcacatgg gccgcgaacg gctcagccgg cactccggct tcgagatgtt cgccgaggtg
    29761 acgcccgagc ggatcgctct gaccgcggcc cgcgaggccg tcgccatgct cgaggccaag
    29821 gacgcgccgg ccgggaagct ggacgtggtc atgcagaacg gctggggcgg cgttctcgtt
    29881 catgaagccg tcggccatcc cctcgaggcc gacaacatcg cccgcaagat cggcgccttc
    29941 accggcaagc tcggccagaa ggtcgccagc gacgtgttca cgatgatcga cgacggaacc
    30001 ctccccaact tccggggaac gaccaactgg gacgacgagg gcacgcccgg caagcgcaac
    30061 gtcctgatcg acaaaggcgt gctggtcaaa tacatgaccg atgtcctttc ggccaagcag
    30121 atcaagatgg agcggaccgg caacggacgg agggagagct tccgctatct gcccattccc
    30181 cggatgacga acaccttcat cgccgccgga aaagacaagc cggccgacat cctggcctcc
    30241 accaagaacg ggatctacgt ccaggccctc agcggcggaa gcgtcgatcc gacgacgggt
    30301 gtcttcaatt tcacctgccg cgaggcctac atgatcgagg acggccggaa aacgacgccg
    30361 gtcaagggcg cgaccctgat cggcaactgc ctggacatca tccagaacat tgacgccgtc
    30421 ggcgacgacc tcgagttcgg ccccggcatc tgcgggaagg gacagtcggc cgaggtgacg
    30481 gccggccagc cgacggtccg cctccggggc atcaacgtcg gcggcagccg ggcccgggcg
    30541 taaggcggaa acgaaggagc aacgaacatg gacatgaaaa tattggcgga aaagctggtc
    30601 aaggactgcc tcaaaaaagg cgctgacgcg gccgaggtgt acatcgagac gggccggagc
    30661 ctgagcctcg aggtccgcaa cggcgaggtc gagacgatcc aggaagcggc cggacagggc
    30721 gtcggcttcc gggtcttcgt ccagggccgg atggccctgg ccggctccaa tgatttcaag
    30781 gacagcgcgc ttgacgacgc catcggccgg gcgatcgagt tcgcccgggt cacgacggcc
    30841 gacgagttca acgtccttcc cgacgatccg ggccagacgg ccgtcgaggg gctctacgac
    30901 ccggagatcg cccgggtctc cacggaccgc aagatcgagc tcctcaagac gctcgagaag
    30961 ctctcgatga aggatccccg gatcaccaag agcgccgggg cgggctacgg ggagggagag
    31021 aacgaggtgt tcatcgccaa ctcccacggc ttgtccaaga ccaccaaggg agccacctgc
    31081 gccatgggaa tttcggtcgt ggccgaaaag ggcgaccaga aatcctccgg cggcgaatac
    31141 tgctcccggc gcttctgggc cgacctcaag ccggccgagg agatcgcggc cgcggccgcc
    31201 cgcaaggcca tcgagcttct cgatccccgg ccgcttaaaa cccagaaggc ggcggtcatt
    31261 ttcgatcccg acgtcgccta tgccgtcctg ggcggcctcc tccaagccgt cgacggcgag
    31321 cgcgtcctgc agggcgcgag cttcctgggg gccaagctca accggaaaat cgcggtcgag
    31381 accatgactc tcgtcgacga cggcacccgg ccccgcggcc tagcgagcgt gccgttcgac
    31441 ggagaaggcg taccgaccca gcggcggctg atcgtcgaca agggcgtcct caggggcttc
    31501 ttgtacaaca cgatcgtggc caagagggcc ggggtcaaga gcaccgggaa cgcctcccgg
    31561 ggcggattct cgagcatccc gggcatcggt ccgcacgcgt tctatatgga agccggcccg
    31621 cacaagcccg aggagatcgt caaggccacc aaggccggcc tatggctcaa ggacgtcacc
    31681 ggttatggca tcaatccggt caacgggaac ttcagcggcg gcgcctcggg tctgtggatc
    31741 gaagacggca aggtcgcctt cccggtcaag ggcgtgacga tcgccgggac ggccgaggag
    31801 atgctcaacg gcatcgatat gatcggcgac gatctcgacc tcaaccgggg actcaccgcc
    31861 ccgacttttc ggatcgccct gatgcagatc ggcggggaat aagcccggcg gggacgggac
    31921 gctctcccgc gaatgaagat gcggcgagga ggtcccgtcc ccggatcatc ggcggcgctc
    31981 cgtccggcgg ccgcggttga cgtccccttt ctcctcgatc cgccggcgga cgccggccgc
    32041 gttcccgaag gacgtccgtt ggggctttcc gtcatcgccg cggtttgaca gccccgccgt
    32101 ttcggtttat aaattctttc tcaatcgagg agatcatccc gaagggatgg ccgcccgctt
    32161 tggccccgga caggacggat tcccaaacgt ccgggatctc cgagaaaacg cctcccggac
    32221 cgcatgcccg gaaggaccgc caacgccgcg gacgccggaa acgatcgaat cgcgagagga
    32281 cgacatggag attcagcttc aagaattgct cgaccgcatc aagcgcgaag gccaggaaga
    32341 agcggagcgc cgagccgcgg agatcctgcg ggaggccgag gaacggaaaa agacgatctt
    32401 ggccgaggcc gagcgcgaag cgaacgagat ccggacgaag gcgaaaaccg cggccgcccg
    32461 ggatgaggaa gccggccggg cctccctggc ccgggcttcc cgcgacctgg tcctggcctt
    32521 ccgcgataag atcgcggcca tcctggacgc cgtcgtccgg aacgccgtgg ccgccgccta
    32581 tgacgaggag atcctccggg ccgccctgcc ggatgttctg aaaagcttgg ccgccggggg
    32641 cgccgaggac ttgtccgttc tgctcgatcc ggaaatgctc cggaaggtgc gggaccggtt
    32701 cgacgcccgc ctcaaggagg agctggccaa gggcttcacc ttcacgccgt tccccggcgt
    32761 cccggcggga ttccgggtgg ccgagaagaa cggcgcggcg tactacgact tttcggcgga
    32821 cgccgtggcc gagctctttt ccaagtacct gaacagccgc ttggccggca tcgtccgcga
    32881 ggcggtcaag gatttgtagg acatgggccg ctattactat ttcgcctcga ctctgccggc
    32941 cctttccctc ggcgccccgc cgccgttgtc gtcggctgat tttctccggc gcggacgcct
    33001 tcatctccgc ccgatggact tcgccctctt ggaaaagacg acgctgcagt ccggcccgga
    33061 tcttccgccg ccggccggct ccggccttct cgaccggtat tacgcttggg agcgatcgct
    33121 gcgcaacgag ctcgtctctc tccgggccgc ccggctcggg cggagcgggg accggtggcg
    33181 tcgtccggcc ggacgggacg acgacgcggc ccgggacgcc cggagcgtct tcctggctgt
    33241 tccggacgcg tccacgccgc tggaggcgga aatcgccttg gaaagcgtgc ggtggatgag
    33301 gatcgaccgg ctccgcggct tgacggtttt cgatttcgac tccctcctcg cctaccgcct
    33361 ccagctcctg attctcgagc gcctggcggt cttgaccgcc gggcgggggg agcggaatta
    33421 ccaggagatt tacgacgcgg tcaaagaggc ggcgcggaca cccgaacaaa ccggagaaac
    33481 cccatgacga agaccagcgg aacggtgacc ggcgtcaacg gcaacatggt caccgtcttc
    33541 acccaaggaa cggtgaccat gaacgaggtc gcctatattc atgtcgacga cacgcgcttg
    33601 aagagcgaag tcatccgcgt ccagggcggg accgtccagg tccaggtgtt cgaaatcacc
    33661 cggggcatca aggtcggcga ccccgtcgat ttcaccggcg agctgctttc cgtcgagctc
    33721 gggccgggcc ttctcggcca gatctacgac ggcctgcaga accccctgcc ccagatcgct
    33781 gaaaaaaccg gcaacttcct cactcccggc gtctatcttg aggcgctgcc ccgaagccgg
    33841 aaatgggctt tcacccccct cgtccaggcg ggcggccggg tggagcgggc cgacgccctg
    33901 ggaacggtgc ccgaaggggc gttctcccac cggatcatgg tcccgttttc cctggccgga
    33961 gcgtggaccg tggccgagat caagccggcc ggcgagtatg ccgtcgacga gcccgtcgcc
    34021 cgcctgacgg acgacggcgg cggatcggtc gatgtgacca tgcgcttcac ctggccggtc
    34081 aagcgggcgg tcgactgcta tgtccagcgt ctccggcccg tccagccgct ggttacgaaa
    34141 acgcgcctca tcgacacgtt cttccccgtc gccaagggcg ggacgtactg cattcccggc
    34201 cccttcggcg ccggcaaaac cgttctccag caggtgacca gccgccatgc cgacgtggac
    34261 atcgtcatca tcgcggcctg cggcgagagg gcgggggagg tcgtcgagac gctcaaggaa
    34321 ttccccgccc tgatcgatcc gcggaccggc cgctcgctca tggagaggac gatcatcatc
    34381 tgcaacacca gctccatgcc ggtcgcggcc cgcgacgcct cggtctacac cgccgtcacc
    34441 ctggccgaat attaccgcca gatgggcctg gacattctcc tcctagccga ttcaacgagc
    34501 cgctgggccc aggccatgcg ggagatgtcc ggccgcttgg aggaaatccc gggcgaagag
    34561 gccttcccgg cttatctcga atcggtgatc gccgcgtttt acgagcgggc cggcgtcgtc
    34621 cggctgaagg acggcgcgac cggctcgatc accatcgggg ggacggtttc gcccgccggc
    34681 ggcaacttcg aggagccggt gacccaagcg acgctcaagg tcgtcggcgc cttccacggg
    34741 ctttcccgcg agcgttcgga cgcccgcaaa tacccggcga tccatcccct cgaatcctgg
    34801 tcgaaatatg acggcatcat cgacggcccg atcatcgatt acggccgggc cttcctccgc
    34861 cagaacgccg aggtcggcca aatgatgaaa gtcgtcggcg aggaggggac gtcgctggac
    34921 gactatatcg tttatctcaa gggcgatctg ctggacgcgg tcttcatcca gcaaaactcg
    34981 ttcgacgccg tggacgcggc cgtcgatccg ccccgtcaaa aggacagcta ccgcctgatc
    35041 atggagatcc tggcggcgga gatcaaggcc aaggacaagg aggacgcccg gcgctggttc
    35101 tccggcctcc gccagaagtt tctcgacctc aacggcgctc cctggcaaag cgagcggtac
    35161 gccgccttgt cggaggagat ccgggccctc ctcgccgaac gccggggcgg cgacgacgaa
    35221 aaagggctgc ggctgatcga ggcgctggag taggtcatga agaaagtgta ttcccggatc
    35281 gaatcgatca ccggaaacgt catcaccgtc cgggccgagg gcgtccgcta cagcgagctg
    35341 gcctcgatcg agacgcggtt cggctcgtct ttggccgagg tcatccgcct cgacggcgat
    35401 cttgtctcgc tccaggtgtt ccagggcggc cgcggcgtct cgaccggcga ctcgctccgc
    35461 ttcctcggcc attcgatgcg ggtgagcttt tccgacaacc tcttgggccg gattttcgac
    35521 ggggccggcg agccccggga cgacggcccg cgcctttcgg aaaacctcat cccgatcggc
    35581 ggcccctccg tcaatcccag caagcggatc atcccccggg agatgatccg gaccggcatc
    35641 ccgatgatcg acctgttcaa caccctggtt aaaagccaga agctgccgct gttttccgtc
    35701 tcgggcgagc cgtacaaccc gctcctggcc cgcatcgccc tgcaggccga ggtggacgtc
    35761 atcatcctcg gcgggatggg cctgacccac gacgattacc tcttcttccg ggacgccctg
    35821 gagcagggcg gagccctggc caaggccatc ctcttcgtcc acaccgcggc cgacccggtc
    35881 gtcgagtgcc tcatggtccc ggacttggcc ttggcggtcg ccgagcgttt cgccctccgg
    35941 ggcaagaacg tcctcgtcct cctgaccgac atgacgaatt tcgccgacgc gatgaaggaa
    36001 atcgccatca cccaggaaaa ggtgccctcc aaccgcggct atcccggcga cctctacacc
    36061 cagctggccg cccggtacga gaaggccgtc gatttcgccg acgccggctc gatcacgatc
    36121 ctggcggcca cgacgatgcc gggcgacgac gtcacccacc cggtgcccga caacacgggc
    36181 tacatcaccg agggccagtt ctacctcaag aacggccgga tcgaaccctt cgggtcgctg
    36241 tcccggctca agcagctggt caacgcgaag acgcggccgg accaccggcc gatcatggac
    36301 gggatgatca agctgtacgc gtcctacaag gaaacgctgg aaaagcgggc catgggcttc
    36361 ctgatgacct cttgggaccg caagctcctc cgctacggcg agctgttcga acgggagatg
    36421 atggacctct cggtcaacat ccgcctcgag aacgccctgg acaaaggctg gcagatcctg
    36481 gccgcctgtt tcgaaccctt ggaaacaggg ctccggaccg agcttctcga aaaatactgg
    36541 ccgaaaaaaa ccgcctgagg cgcccgcgat ggccaaggtc aagatgacga aaaacgagct
    36601 caagaggcag cgggacgccc tcaagacgta cctccgctac ctgcccactt tgcagatgaa
    36661 gaaacagcag ctgcaggtgg agctccggat cgtcgaagcc cgccggcgcg agctccggac
    36721 ggcccgcgag gagctcgacc gggaattccg gacctggatc gcggtctttg gagagtcatc
    36781 cgccctgtgc ggccccgacg gccggccgtt tctctccgtc gccgccgtcc gcacgtcccg
    36841 gggcaacgtg gccggggtgg acatcccggt cttcgaggga gcggatttcg cggtcgccga
    36901 atatgatttc ttcaccgccc ccctgtgggt ggacgcggcc gtcgagcgga tgaaaaagat
    36961 ccttcttgtc gacctggagg acgacgtcct ggccgggcag gccggccggc ttgcggccga
    37021 gctgcggacg acgtcccagc gggtcaacct gttcgaaaag gtcaagatcc ccgaaacgct
    37081 ggagaatatc cggatcatcc ggatctccct cggcgaccag cagatcgccc aggtcgtgcg
    37141 gggcaagatc gcccagcgga aagtcatcgc caggacggcc gcatgatcgc ccggatgaag
    37201 aaggtttggc tcgttctcct ggaaaaggag aaagccgggg cgctccgcga gctgcgcgat
    37261 ctcggcgtgg tccacgtcga atgcgtccgg ccgtccggcg ggcggttcga gcgcctggcc
    37321 cgaacccggg acgaagtcgc ggccgccctc aacatcattc cggcggagaa acgcctggcg
    37381 ccgggccggc tgtcgataga tgaggcgctc gccctggccc gccggatcct ggccggggag
    37441 gagcgtgtcc gggaaatcca ggacgccggg gccgctctcc aaagggagat cgaccgggcg
    37501 gcggcttggg gcgaattcga cccggaggcg atcgagggct tgcgggcttc ggggacggac
    37561 atccgtttat tcgaggtgga tccgaaaaaa cgggcgaccg ccccggccgg gacggacatc
    37621 gtcgtcctgt cggccgaccg gcgccggctg ctcgcggccg ccgtcggccg gcggggcgag
    37681 cccgttccgc cgctgccgcc ggcgtttgtt gaattcgttc cgccgcttct ttcggccgcg
    37741 gccatgcggg aaaaaatcgc ggccggcgag gcggagatcg cggagatcgg gaaaaagcgg
    37801 gtcgccgcgg gccgggagag agattcgctg caacgggcgc tcgaaatcct cgagcaggac
    37861 ctgagcttcg agacggtacg ggaatccttc atcggcgaag ggcctgtttg ccacgtcaag
    37921 ggatacgtcc cggcggccga cctgggcgcc ttgtccgtcc gggcgaaaga ccacggctgg
    37981 gcgttcgccg ccgccgaccc ggaacccggg gatcaggtcc cgaccaaggt cgagaacggc
    38041 cggttcgtcc ggatgatcga gccggtgttc gaattcctgg ggaccgttcc cggctatcac
    38101 gaattcgaga tctcgttttg gttcctggtg tttttcgccc tgttcttcgc catgattttc
    38161 ggcgacgcgg gctacggact cctgatgatg ggcgccggcc tcgccgccgc gctcaaggcc
    38221 cggcgcaagt cccggaccgt ccccgacggc gtccgccttc tcctctttct ttccggggct
    38281 ctcattctct ggggcgcggc gaccgggaac tggttttcga ttcccttcgc ctcgcttccg
    38341 ccggctctcc gggcgatcgc cgtcaagccg atctgcatcg ccaacccgga cgccaaggcg
    38401 aacatccagg tcttttgctt cctggtgggc gtcgtccagc tgtccctggc ccggatcaag
    38461 aatatccgcc gcttgtttcc gaatcttcag ttcctcgccc acctgggctc gctggcgatg
    38521 atcatcggaa tgctgttttt cgtcctcaat ctcgtcgtcg acgcgaagcg ctttcccctc
    38581 ccggcgtacg ccgtcggact ggtcgtcggc ggattcgccg ccaaccttct cttcggcgcg
    38641 tatcaaggga acctgctcaa atcgcttctc ggggggcttc agaattttat cccccttttc
    38701 ctcggcacgg tgggcgtgtt cgccgacatc gtgtcctata tccggctttg ggccgtcggc
    38761 ctggccggct cgtcgctcgg gtcggtgatc aacggcatgg gcggcggctt gatcaagccc
    38821 gccctcacga tcatcgccgg agtgcttctc ctgacgttcg gccatgccct caatatcgcc
    38881 ctgagcgtgc tgtccgtcgt cgtccacggc atccggctca acatgctcga attctccaac
    38941 catctgggca tggagtggtc ggggatcaaa tacgaccctt tccgggtcac ggccaagtcg
    39001 gagcggtaat ccagtaagga ggcggaaatg gatctcatcg gttatatcgg cgtcgccgcc
    39061 gttttaggcc tggcggcggt cggctcgggc atcggcgcgg gcatcgccgg gcaggcggcc
    39121 atcggcgcct ggaagcgcag ctatctggcc aacaagcccg cgtcctttct gctcgtcgtt
    39181 ttcgccgggg cgcccctcac ccagacgatc tacggcttta tcgtcatgaa cgccctcaag
    39241 ggggcgggca tcgacggctt gttcaagctg gggatcggcg tcctctcggg ggcggcgatc
    39301 ggctattcgg ccgtggccca ggggcaatgc tcggccgcca gctgcgacgc gttcggcgag
    39361 acgaacaagg gattcgccca ggacctgatc gtcgtcgggc tctgcgagac cgtggccatc
    39421 tttgtcatgg ccttttccct ggcgatcatc ggttgaggcg ggcgccggtc cggaaggcga
    39481 tcactccgta ctcctccccg ttcggacgga aaacgcgctc gactttgaag ccggtcgcgg
    39541 ccaggaggcg ccgcagctcc cggaggggga ttcggtcgcg gagcggggga ccgtgctccg
    39601 ttttcttccg gagaaagtcg atgatgacga gacgggaggc ggcggacatg cgcttgcgga
    39661 gatcgcggag ataagcttcc cggccgtcga tctcgtggag gacgttggcc agaaaataaa
    39721 aatccgcccc gggcggaacg ttcgggctcg ttttggatat tcggcgggct tggaggttgg
    39781 ccgctttggc ccgggcggcg tttttccgaa gctcttcgac catcgacggc gaaacgtcca
    39841 gccccacgac ccggcccctc ggtccgacgg ccgcggccgc cggcagggcg aaaaaccccg
    39901 gaccggcgcc gatgtcgatg aagacctggc ccggacggag gccgatcgtc tccagaacgc
    39961 gctcgggggg aatctcctgc cggcgctcgg ccgacatcag ccgtttcgac cgggcggcgg
    40021 cggatttccc gggcatgacc atcctcaaaa acaggagcga tcgggagaca caatacccat
    40081 gagtctaatg acgatttcaa ggaaaaagct aatccgaaaa cagggccatg tcaaataatg
    40141 ttcgccgagc ggagcatcgg agaaaagtcc ttctttcccc cttcggttgc atattcgttg
    40201 attcaatgcg ctcatttggc ctggtgttct ttgagaatga tccttcatat ctcaaacaac
    40261 cgtcgtctcc gggccccggc gaaaataggg tgaatgggga tggcgtctcc ccatgaaatc
    40321 agcgggagag gaatcccagc acctcgaagt gcggggtgtg gggaaaaaag tccgcggcga
    40381 agatgcgctc aggccggtag cccgaagccg ccagcccccg gatgtcccgg gcgaaggagg
    40441 ccgggttgca ggacatgtag atcagatggg gcgctcccag cgccgggatg agagtcaatc
    40501 cggcgggatg aaggccgtcc cggggcggat cgaggacgac gaggtcggtc ccggagaggt
    40561 ccgtgctttc gaggactttt tccaccagcc cggcgatgaa gcggacgttc cgtaaaccat
    40621 tgcggacggc gtttccctcg gcgctctgga tgttggccgg ctcggcgtcg acgccgacga
    40681 cttctgcggc cgtccggctg agaaacatct cgatcgcccc cgggccgcag aaaaggccga
    40741 gcgctttttt cgcggagacc tcgaccgccc gggcggcgat tttttcatag aaaacggccg
    40801 ctcccttcgg attgggctgg aaaaaagaat ccggaccgat ccgaaagctc agaccggaaa
    40861 gttcctcctc gatcccggcc gcccctacga gcagggtgtt gttcccgtat tccacgaggt
    40921 cggcgtcccg gtcgttttcg acgtgccaaa cgctccggac ggccgggatg tccgcggcca
    40981 gccggcccgc ccactcccgg agtccgggcg ccgggccgct tcgggtgacg aggatgacca
    41041 tcacctcgcc cgtgcccttg gcctcccgga ggacgaggtt ccggaagaat cctttccggg
    41101 agaccgggtc gaagcccggc cgtccgtcgg cttcggcgaa cgccgcggcc gccggcagca
    41161 ggcggtcggc cgccggtccg aacaaggggc acgtcctgag gtcgaccgtc ctcttacggg
    41221 acttcgtccg cgggagggat cgttcgcgca gtcccaggcg aaggtccccg ccggctccgg
    41281 caaaggcgaa ttccatcttg ttccgatagc cgaaaaggga cggcgagggc aggaagggct
    41341 caagaacctc cgccgccccg ggcggtccct cctttcccaa cacccggcga agatagcctt
    41401 ccttgagcga gatctgttcg gagtagttca ggttttggaa agcgcagccg ccgcaggctc
    41461 cgaaatgagg gcaaacggcc tccgtccgca ggggcgacgg ctcgaggagg cgggtgattt
    41521 tcgccacggc cgggttgcgg cgcagaattc gcgccctcac ccggtcgccg acgacggccc
    41581 ccggcaccca gacgtccttt tcccccgccc gggcgaccgg ccagcttttc gggaaggcga
    41641 tcccagtgat ttcgagctcc atctccgggg ccttgaattc cttcagcagc tttttaaagc
    41701 gcttcacggg aaatccgacc acgttgtcgt aatcgccctc gaggcgctcg acgaacgcgt
    41761 ccccgacgtc ctggacggcg tagctgccgg ccttgtcggc gtaggcgttc cggtcgagat
    41821 aggcctcgat ctcctccggg gccagggcct taaacgtgac atgcgtcgtc tcgtaaccga
    41881 cgacgagacg gtcgtcggcc gcccggaaga gggcgactcc cgtcaggacg cggtgacgcc
    41941 ggccggacaa gcggccgagc atcgccgcgg cgtcctcccg ggtttccggc ttgccgaaga
    42001 tctcccggtc caggcagacg accgtgtcgg cggcgatgac cagggaatcc gggaagcgcg
    42061 ccccgacgtc gcgggctttg aggacggccg cttcccggac gaagaagacc ggatcgcttt
    42121 ggtcgagggc gctctcgtcc acgccgctgg gaatgacgcg gaagtcgggg aagatccggc
    42181 cgaggatctc cttccggcgg ggcgaccgcg aggccaggat gacgtccacg acgctcaatc
    42241 gcggacgagg gtgacgcgga ccgggcggac tttgccggcc cggtccacgc tcaaggtcac
    42301 ggtttccccg atcttgcggc tttccagaat ggcgaacaag tcgtcatagg acgtgaccgg
    42361 ggcccggtcg acggccgtga tgacgtcgtt gatggtccgg cggccgaagc gatcccggcc
    42421 caggccgcgg agtccggcgg cgtacgccgg ggttccggac gggacttcca ggatgatcac
    42481 cccctcgatg ccggcccgcc gggcataggc gtcgttgagg aaggagacgc ccagtcctgg
    42541 ccgggtgacc cggccgtggg cgatcaattc gggcacgacc ttggcgatga tgtccgccgg
    42601 cacggcgaat ccgacgccgc tggaggcgcc cgagggggaa tagatgagcg tgttcatccc
    42661 gatgagccgg ccggctgaat cgagcagcgg ccctcccgaa ttgccgggat tgatcgaggc
    42721 gtcggtttga atcatgtccc ggatggacac gccgccggcg ccggtgattt tccggcccag
    42781 ggcgctgacg atgcccttgg tcacggtata gtcgaagccg aacggattgc cgatggcgat
    42841 cgtcttctgc ccgacgacga gatcacggga tgtgcccggg acgaccgggg agagctcgga
    42901 cacatcgccg tcgagccgga gcacggcgat gtccttggat tcgtccttgc cgacgagccg
    42961 ggccgagcgc tgctgctggt cgggcagagt cacctggaac tccaccccgt cctcgatcac
    43021 gtggaaattc gtgacgatgt gcccccgccg gtcccagacg aaaccggagc cgctgccgcg
    43081 ggggatcttt tcctcggctc cgaaaaagaa gtcgcggacg agctggatgt tggtgatgta
    43141 cacgaccgaa ttcctcgttt tgttgaccac ttcgatcgtg ttcctttcgt cctcggtcag
    43201 gggagcctgc tgggagtgaa ggaggatggg gccgccgaga agcaggagga gggccgcggt
    43261 ccgcgcccgc cggggggagg ttggtttctt catggtcttc tccattgaag ccggattata
    43321 tcaccggacg gccgcggtga aaaaacaggc tcaggccggc gtccctcgct ccggcggcct
    43381 gcggaccagg gcccctcgtc cgatcatcac caggttcttg atgagatcga tcgccgtcat
    43441 caggacgatg aagaggaggg cggccgccag gccgattctg attcccgaca aggcttgtcc
    43501 gctgatcggg acggcgaaca agccgtcgaa gggccggcgg aggatcaggc cgaacaggat
    43561 cagggagacg gcgtcggaac cgacgtcgat ccagcgggac cgggtgaaga gcttgagaaa
    43621 cagggagacg gccccggccg ccagcagcgc gatcacgatc agcgacaaac tccggccggc
    43681 ttccgccgtg aaaaggggct tgatttcccc cgagctcgcc aacccggcga tgaataccgt
    43741 cccgtgccgg gtgaaaacat agaccgccgc ggccgtcaag gcgagcatga acgtcaagcc
    43801 ggcgagcgtc gcggcgaggg ccccgggctt ccgggggagc ttgatgtcat cggcctcgag
    43861 cccgaatttc ggccagggga gcttgatgtc ctttttactc tgggtgacga agtagaggat
    43921 cagggcgaca aacccgagat cggtcaaaaa cgccgccggg agatacaaca gagcctcgat
    43981 gacgcccatc cggggcacga aaagaacggg aaatagaacg aagctccggc cgaagatcac
    44041 tccgatcagg gtgaaaagaa aatggaagcc gaacaggatg gaggtgtatc ggaacaggtg
    44101 gcgctggtag gccggggcga tgatctcccg gccttccaag taccgctcgg ccacccgtcg
    44161 gggagggccg aacccggcga tgactctttc gagggcggcc tcgccgctcc gggcgcgctc
    44221 ctcctcggct ttttccagga tgtggctccg gatttccgag aggatctcct cgcgggccgg
    44281 gccgacggcc aggtaatggc cgatgtcctc gaggtaggca tcgagattat tggtcatgtt
    44341 cgttctcctt ctcgatttct tcgagaatcc tattttggtt tttccagacg gcgagaaggc
    44401 cggctttcat cgcccgcccc tcggccgtga tgccgtagaa cttgcgcggc cggtcttccc
    44461 ccagcgtcca ccgcccggcg atccagccgt ttttctccag ccggcggagc aggggataca
    44521 gggtattttc ctcgatcttg tatcccagcc cctcgatgtg cttgatcatg gagtagccgt
    44581 agcggtcttt ctcgaggagg gcgaggacga gaatttggag gaatccccgg ttcatttccg
    44641 tttcgagact tccgagttcc gtttccgtca ttgtgatcgc ctcgtttttt tcttttacac
    44701 gctcgaaaac aatatagtgc gttagacaat actatatcaa acacagtatg tcaagcttcc
    44761 cgcgttttct tcacgggaag ggggctggcg gtggcgtttt taacgaccgg agcgaacatc
    44821 atccggatgc tccattcctt gagtcttgtc tgggaaatcg gcgcgaaaaa gaccggcgtc
    44881 agctgttgga gactgccggg cgccggcaaa aagccggggc gggcggcggg gcgaatcttt
    44941 caggacatgg attctccgcc gccaagccgg tcgatcggga aaatccgcga tgaatccggc
    45001 gcgggagcga aggctttcat tatcttgccg ctttcctctg tgcaaatggg acgagagccg
    45061 gcttttttga gatcgattca cgggtttgac agccccgcgg acgtatggca ggatgaatcc
    45121 gccggagccg acgccggcga ttcggcggga gaaattcagt tgttttccga aggagccgtc
    45181 atgctcgacg tcatcgtgaa actcgtcctg gccgtcgttc tgggcggggt gatcggattg
    45241 gaaagggagt tcggccagaa gccggccggc ctacggacca atatcctcat ctgcgccggc
    45301 tccaccatga tgatggccct ttccggcatc atcctcggcg gagcccccgt cgggtctgcc
    45361 gattccctgc ggatcgcctc cggcgtcatc accggcatgg gcttcatcgg ggccgggacg
    45421 atcatccagg cccaccggca cgtccatggt ttgaccacgg cttcgacctt gtgggcggtc
    45481 gccgggctcg gtctggtcat cggggcggga tatttcttaa tcgccgcgat ctacaccgga
    45541 atcctgacga tcgttctcct gagcttcccc ctggtcgaac acctcgtccc gcaccggtcc
    45601 atcgtccgtt acaatctccg ggtggccggg atcgaggcgg tggacgagtt gcgcgggctg
    45661 gccgccgcca cgggcgaaaa agtcgacgaa atcttcttcc gccgggaaac gaacggggcc
    45721 gttctcgtgt tcgtcataac ggaacgcccc tcccgggccc ggaccttcag ggataaaatc
    45781 gccgccctgg gggaaatcgt cgagatccgg atcgattagc ctgcgtccgg aggctcgtcg
    45841 atcggttttt ccgtggtcgg attccggaag gccttccagg tccaaacccg ggtttccccg
    45901 tcgacgaccg aacggatggt cccattgatg gactggccca cgagcttgcc ttcaaagaca
    45961 aaggccgccg gggaaccggc gacggccgcc tcgaatctga accggacgag gtctccctgg
    46021 atcttcgcgt cggcgatcgc cgcctcggtt ccgccgattt tcaccgttcc cttggcatac
    46081 tgatattttt ggatcagatc ggcccggacg gtcgttgttt ttcccttgat cttcatcgtc
    46141 caggtccagg cgccgctggc gttggccggg atcacccaga gaaacacgtc atggcggacg
    46201 ccgtccactt ccacttccga ggaggcgtcc ggcttccagt tttccatggc gaaattgtgc
    46261 gaaacgaccc gggatcccgg ccgcagctcc cggagcaggc gcggccgaag ccggaggttg
    46321 atcgaagtca ggaggtagag ggagacgacc gtcgcttcgc ggatatcggc ttcgaataag
    46381 tcgccggtgt agaacctgac ccgatcggcg acgccggcgc tggaggcgtt ggcttcgctt
    46441 tccagaatgc gttccggatc aagatcgatg ccgatccccc gggcgccgta tttctgggcc
    46501 gccccgatca cgatcctccc gtccccgcat cccaagtcgt agacgatgtc gttcttgctc
    46561 actccggcca tgctcagcat ttcgtcgacg accgggaact tggtcggaac atagggaaca
    46621 tccaggagcc ggacctggcc ggcggcgccg gagatcagaa gaaggagggc ggccggaacg
    46681 gcgaacgagg agagacgttt ggtcatgggg gggtcgatcc ggaaaaaaga tagcacaggg
    46741 cccgggcgaa gacaaggg
//
LOCUS       DAOOXH010000010        42768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_748_length_42768_cov_7.565846, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000010 DAOOXH010000000
VERSION     DAOOXH010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 42768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42768
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_748_length_42768_cov_7.565846"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PKV64_02495"
     CDS             complement(<1..312)
                     /locus_tag="PKV64_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HPN15983.1"
                     /translation="MSFTRLNRSAATLTKNRTEGSISPFSGMCVTCVDGCIGMCEIGK
                     SAYRAAEVLYPQPFGIITAGSEKEYPFDLSHFSIMGTAAGAYGCEADSDKATFEKVST
                     ET"
     gene            complement(342..1025)
                     /locus_tag="PKV64_02500"
     CDS             complement(342..1025)
                     /locus_tag="PKV64_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPN15984.1"
                     /translation="MIPILMNGLEVQAEEHWTVLEAARFYGLEIPTLCWMEGLTPWGG
                     CRLCLVEIGEPPKSRLVSSCTYPVEKGLAVRTDTRRVIEARRVMIELMLSTAPMSKKI
                     QDLASKFGVTQQRFPPRNEECVYCGLCIRMCDEQMDARAIGFQNRGYKRKISTPFDIR
                     SEECRLCGGCLYVCPACSARCGGPNADTDICNACLQIEPTCLEQYDELNCWMYDVGRC
                     GSCVQEKKR"
     gene            complement(1022..4186)
                     /locus_tag="PKV64_02505"
     CDS             complement(1022..4186)
                     /locus_tag="PKV64_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPN15985.1"
                     /translation="MRVVPIKTLAVLEQTIAAHERLRKKDRRPILTVSAGTCGRARGA
                     LKVVDALRTSLRKAKLDKKVRLRVTGCHGYCEAEPNIIVQPHDLFYQKVDPKDAALVV
                     SETIRKKKPVPELFFHHPATGKPAKRERDIPFYAKQKRLVLGDNARIDPTSIDDYFSI
                     GGYSALAKVLRKLGPEDIIAEIKASGLRGRGGAGFPTALKWDLTRKQPGSVKYVICNA
                     DEGDPGAYMDRSLLEGNPHRVLEGIMIGAFAIGATEGVIYVRDEYPLAVKHVTIAIEA
                     LRKRRLLGPDILGAGFSFDFRIVKGAGAFVCGEETAMIASIEGRTGEPRQRPPYPSVR
                     GLNGKPTVINNVETWGNVPIIISGGGAAFAKVGTPGSKGTKIFSLVGKINNTGLVEVP
                     MGLALKEIIYDIGGGIPGGRRFKAVQTGGPSGGCLPAGKLDLPIDYEGLAAAGSIMGS
                     GGMIVMDEKTCMVDLARYFLNFLRDESCGKCVSCREGTQRMWEIVSRIAEGQGREEDI
                     GTLEDLAAACRDASMCGLGQTAANPVLSTLRYFKDEYRQHIRRKKCPAQVCKEIVSSP
                     CQYVCPIGQEASTYIALIASGRYEEALRVIMKDNPLPSVCARVCHHPCELACRAGESG
                     EPISIRGLKRFVADWAGKNGIRYVPPKIEQNREKIAIIGSGPAGLTAGYYLALKGFRP
                     TIFEALSVAGGMMAVGIPDHRLPGPLVQREIADIKNAGVEIRLNKTLGRDITIDGLFK
                     SGYKAVYCATGAGKSLRMGIPGEDAEGVLHSLEYLRQVNMGKTVPIGQRVVVVGGGNS
                     AVDAARVALRDPDCEKVTIFYRRTKAEMPAIAEDVEAAVEEGVDIRFLVTPVKVLMSF
                     GKAVGIECQKMKLGEPDASGRPRPVPVEGSNFVHKLDTLILAISERPDTSYIGEGDEI
                     TRHGENIVIDAETAMTTRAGVFAGGDAVTGPNMVVDAMAAGKRAAEMIAKYVEGKALK
                     PDYKLSRPSLYAAPPALSDEEIQKARRPAMNRLSTTDRRNNFREVELGLTERAAVREA
                     RRCLRCDLETEDGKAALKAMKEKP"
     gene            complement(4192..4656)
                     /gene="nuoE"
                     /locus_tag="PKV64_02510"
     CDS             complement(4192..4656)
                     /gene="nuoE"
                     /locus_tag="PKV64_02510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPN15986.1"
                     /translation="MDVQIDRVNAFIRKHQNQKKALIAILQDVQAEYSFLPQEALRQV
                     ARSVGLPLIDVVGVATFFRAFSLKPRGRHVVTVCSGTACHVRGGPKILEEFERQLGVG
                     PGETTPDRKFTLETVACLGCCAIGPVVVVDGDFHAQTSTRKVAAIIKQYGKK"
     gene            4950..5360
                     /locus_tag="PKV64_02515"
     CDS             4950..5360
                     /locus_tag="PKV64_02515"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HPN15987.1"
                     /translation="MRHEILSDESLEIMREIWARPEASINDVWTALNAARRKKVRRTT
                     VQVQMTRLVRYGWLSRRKSGREYLYAALYGKDETVGDLLSALARRVYGGSFVELVGLL
                     LERGIFSPADRRRINVMWRKIRRGKETGPRLFPA"
     gene            complement(5383..6468)
                     /locus_tag="PKV64_02520"
     CDS             complement(5383..6468)
                     /locus_tag="PKV64_02520"
                     /inference="COORDINATES: protein motif:HMM:NF023327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HPN15988.1"
                     /translation="MSRLEVRYAQCWEDPRLSREALAVGPDDDVLSIASGGENGLAFL
                     LDRPRSVTLIDRNPAQIHLVELKVLAARRLDREELLGFLGARPGPDKDRLYDVLRPGL
                     SGPARAFWDRRRDLIRDGLFHCGRYERFFRAAIRYVVPLIHSRKTLRALLDCPTVGEQ
                     AAFYRDVWTNRRWRTLFRLVFGKRVFGRWGRQRSHYRYVTAADTWRLFYDRAERELSQ
                     RLVRDHPGLAYFFKESAYPRPENLPLWLQAAHFPAIREGLDRLKLRTAGLDEILAATH
                     PGDFSKFNLSNIFEYMAAGEYARALGGILRVSRSGGRLAFWTMFVPRPVPSSLSARFA
                     PVFPDGSPDPAYRTWTLFEDFHAWRLL"
     gene            complement(6465..7595)
                     /locus_tag="PKV64_02525"
     CDS             complement(6465..7595)
                     /locus_tag="PKV64_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15989.1"
                     /translation="MSLRSFRFDPSLTREFIAFGGSLYAGGARRVPPFRREIIRQLSP
                     DFYFYQRAGNDHRRFLAYRAGRVAGRIAAMVNAEIRDGDRLPVGLVGFYECVDDDGVA
                     AELLEAAREWLRTEHGVRRIRGPMNFDFWHGYRFLTKGFDREPYYGEPDNPAYYPEQF
                     ELGGFAPLRAWNSIEINGREKLEALIAPWRKRHELLLERGYRFVPATRLDLRKEWPAL
                     HGLIDRSFDGFPDYTPIPFTEFKALLSGVGFALAADVSGFVYDQAGRAVSFGVIFRDL
                     AGAVRAMNGRITPLGALRFLSRLRKRRRFNFFAAGSLPEEEARGTGVGRAGFFAMIDE
                     SLRRGYSDFIFAQMPKDQPIQAFFRRLKIEPQREYTLYEREA"
     gene            complement(7686..8615)
                     /locus_tag="PKV64_02530"
     CDS             complement(7686..8615)
                     /locus_tag="PKV64_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HPN15990.1"
                     /translation="MSDADRLEYYDRREGRVKSDAVPAARLTHWIHNTRLGRRAGEVL
                     FGGAAASRLYGRFQKSRLSRRRIGPFMRTYGVDPADLAKPAREYASLRDFFVREIDPA
                     RRPFVPGSEACAAPADGRILAYPDLPVDRVFPVKRALFNLRDFLRDDRLAEDFAGGTL
                     VVSRLGMGDYHYVHFPCSGIPAPAISLPGKYRPSGPYSLKRTVPYFRENHRMITLFET
                     ERFGRAAIIEVGAFAVGSIRQVFRPGVPAAKGDKKARFEPGGSTVALLFRKGMIEVDA
                     DLLARTAEGMETYVRLGDSIGRAAAASNPRPAP"
     gene            8768..9091
                     /locus_tag="PKV64_02535"
     CDS             8768..9091
                     /locus_tag="PKV64_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HPN15991.1"
                     /translation="MISRIWHGWTTRDNADQYEKLLKEEIFEGILNRNIRGFKSIQLL
                     RRDLGREIEFVTIMTFDSLDAVREFAGEDYEAAVVPKSARAVLSHFDDRSRHYEIIIE
                     RTGEK"
     gene            9141..11192
                     /locus_tag="PKV64_02540"
     CDS             9141..11192
                     /locus_tag="PKV64_02540"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPN15992.1"
                     /translation="MRVSRKTVFTSGSVALILWLCLAFAFAQAPAKRPLAIDDVLGGL
                     EISDPQVSPDGGWIAYTVAWMDRQADKARSAVWMVDWEGRNNVPMTSGPEGDTAPRWS
                     PDGKYLSFLSTRPADGKTQVWLLDRRGGEARPLTDVKGELSAYEWSPDGKRLVLVMQE
                     GEEIPDEAADSKAKTAKPIVIDRYAFKRDWVGYLTASHRDRLYLFDVGTKTLAALTAK
                     TNHDESDPAWSPDGSRIAFVRGHGEDSDRTGMSDVFIVEARAGAEPRQVATIGAPDRQ
                     RLVWSPDGRTLAFTRGFEPKYYAYNQDRLAVVPVEGGEPRVLTAELDRGVSQAEFTPD
                     GSALTFLVEDDQRTYAAEVPAVGGAVKRVSGGIEVISEIARGGGRTAVAAATDAAATE
                     IYAFENGALRKLTAHNDALFAGLKLGAVEDLSFKSKDGTAIRGLMVKPPDYEPGEKYP
                     TLLWIHGGPNMQDDHSLTFGLYPLQLERQLFAARGYVVLAVNYRGGSGFGAAFTRSIF
                     ADWGAKEVDDLLAGADEAVRMGVADPDRLGIGGWSYGGILTNYTIAADGRFKAAVSGA
                     GSSNQIAMYGVDQYTMQYDNELGPPWRAPEAWAKVSTPFLRADRIHTPTLFMGGEKDF
                     NVPISGSEQMYQALRTLGVPTQLVVYPGQYHLLTKPSYIRDRLERTMAWFDAYLKAPQ
                     K"
     gene            complement(11232..12629)
                     /locus_tag="PKV64_02545"
     CDS             complement(11232..12629)
                     /locus_tag="PKV64_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPN15993.1"
                     /translation="MGRDFLRSGFIARLAASPRFPSAARLFTLAAFLLLIAGGLAVPH
                     VSPGLTGILRNTSLASLVVWSLWWPLVIMSAAVLGRVWCTVCPMEMVASACSRIGLKK
                     RIPRFLASGRGVVFFYILALLGFIRTFWAHRYPERMALFFLFLLGSAAAAGFLFNRRA
                     FCSHLCPVGRILGLYACGAALEWRVRDPGICAACGGKNCLSRPEAGFGPAATCPSGLH
                     PAAIRDNRDCLVCTECLKACPSRNLRLSLRKPLSDFFTGLRLRNTDLFLLVPVSGLVV
                     WELAEEWGPARKALEAVPAAANAALGLSGGAANFVQAVILFLLFPALLLLIPAAAGRM
                     LNKTTLLESARTFGLLLLPVTALGHLVKALIRIASRLPYYAPAFRDPAGLETARAIAA
                     GSLKMDNRLAAAVAPAVSILAVLALAGAVVSIGLIVRKSPVLRAFGKAGRLSHGAFAA
                     LYGLALLAAAVLARF"
     gene            complement(12641..14032)
                     /locus_tag="PKV64_02550"
     CDS             complement(12641..14032)
                     /locus_tag="PKV64_02550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPN15994.1"
                     /translation="METLRFDAVVIGGGEAGLAAAVKAAELGARVGLIESSSQLGGAC
                     VATGTLPSKTFSISTGMFETIKKAKSFGVRIDGPVSLDFQEVVASRNRLMKCDIGVIQ
                     SHLRSRKVTLVSGRAAFAAPDRIRVARADGTEAVIESSRTIVAAGSHPIHLPVLPVDG
                     ATVITTDEIVALASCPPRIVIIGAGIIGCEYAFIFRTFGADVTLIEKTGRALPGQDKD
                     VVAAVEKELKRRGVALRFGTSVSACAAGLEGGRLVTTESGETFAADLVLTCVGRRPST
                     EDLGLEAAGVAVGGRGEIIVDECLETTGTGIFAAGDVLGRRMQSSTAILEGAVAAENA
                     LGGSRTIDERFVPGGIYTQPEIGSVGLTEDEAAAKGIPLIVGFCPYASLVKACSLQTF
                     NPGFIKMLFDPSSHKLIGAHILGPESAEIIHNLVVAMRTGATAEDFVYSIYHHPSLSE
                     GFREAARDALSKI"
     gene            14158..14439
                     /locus_tag="PKV64_02555"
     CDS             14158..14439
                     /locus_tag="PKV64_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15995.1"
                     /translation="MSACRLASLNRRAAQGVLLGLILSLTSALAQLPPASPAEAGLSA
                     ERLERLSLYLQPVVDEQKAAGRGRSGRTAGEARTTRPIGSIPGSRWSAS"
     gene            14721..17984
                     /locus_tag="PKV64_02560"
     CDS             14721..17984
                     /locus_tag="PKV64_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPN15996.1"
                     /translation="MVGWPEMGSEFKLTRASAGSGKTYALSLRFARFLLTDGPPGVPR
                     DLPHILAITFTRNAAREMKERILDWLKEGYRGEGKKAEALRVKLHQEFHERLFSPVEL
                     SRRSEAAVERILAGYTDFQVDTIDSFAAMVFRASAVDLGYGPDFEISLDPGELVDYAF
                     ARFLRRVRPESAEGEVFRRILDYLLVQESEKARFLWDPAPKILERLKAFQKKLTARPG
                     ELVLDDFQEEKAKLGKTIRGTAGRLRGAVEESGLDLSARGHYRGRIMPAIEDGRFADL
                     LDCSFGTLPVKGGSKAKGAAAEALARIEKEWKALEGLVGEYARLHARDFFLPYLRAYQ
                     SVQAEMKAAKLGRGLLFLDDVNRELLHYLERGIVPDVYFRLGDRIYHYLIDEFQDTSP
                     IQWRILKPLIEESLSRGGSLLIVGDAKQAIYGFRDADFRIMRDLEEGADGFASAPVSP
                     DDLEMNYRSEGLILDFVKEVFLGLASGSGGEGEKSGVDYGRLAGLSGLDHFTQSVLPE
                     REGRGYVRYILFDKGGAAAETGEGEEENSGEPGGDSEAPEKSEIQTLIKELLGRGWRR
                     ADLAVLAGRNNEVAAVASWLNEIDIPFVSYSSLDIRRRKIIGEILSFLRFLDSPPDNL
                     AFAEFLLGDLFTKVAGKEFDLSARREFLQSCARGDGGPRYAALRRVRPGLWEEYFEPF
                     FKSVGYLPLYDLVTRMYRQFDVFGLFPGEEAALSKLLEAIKTFEGEGRNDLREFLAFS
                     ADEEDSDENWTIDVPREMDAVKIMSIHKAKGATIPVVICLFYGQSFRGEEFYLHDEED
                     GVHVLKINKKLAESEASLSAAYGEALDREWVDRLNTLYVALTRAQYELYIVGVKGKKG
                     KFPFDLLERAFPSSTEEPVVHADGTRLNRSFREGEPAGPESYLRAKGRDEEAGESGLI
                     REITHRAGFTEPPVNMRTSLKSAGIRRGEWIHRLLAGVELNRAGWDAEVAAALEGLGL
                     SPAERTAAGEAADVLRRAFGKSIAADWFEPRPDRFILREADICDPEGDVFRMDRVVLD
                     PGLVLVIDFKTGGRTEEAEAYREQIRRYVRLLRDVHPGRPVRGFLSFVDRDRIEEVE"
     gene            17981..20923
                     /locus_tag="PKV64_02565"
     CDS             17981..20923
                     /locus_tag="PKV64_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPN15997.1"
                     /translation="MSVGRIRLVPPRESLIEAVAGLLPSGGRDLSRSWIVFPERRPAY
                     YLRKHLAGRIGAGFIPPRIFSVDAFTDFVYREGLGRRNRPLDVLDAVAILLGIQRSAP
                     GRLGGERFLTADHFFPLGVKLFRDLEELIAAGFTGASLKEKGLDQAVQDAISGPGEDL
                     LTDRSRNRLQDLSYFLEKFLAAVEAAGFSTPALRLRAAADGIDRGRFAGADRFIFAGF
                     FSLNAAEEKLIRRMLGWEDFTLLAHQAAGVERLLERLGLEPAAAAGGGPAGGSLPEIT
                     FTRCPDAHGQVFALNSLLAPKLAEPALFNERQVVVLPAAETLFPLSHQTLDDLAPEEY
                     NISLGYPLTRTPVFTFFERLLQVIQSADAEGRIYAPDYLRFVLHPYTKNIQFPGPGRR
                     SDLTRILFHAVEEALTEKKGRAFWALEEIAVEPAVREAVARLSRGVEGAPVPADFLDH
                     LRTIHEALLEPLRTIASVGDFAGKLSRVLDFVYENGTARRHYFFHPYAEAFCARLERL
                     AGSLLRDMVFEEREGYFNLFRKMAAAGSVPFFGTPLRGLQVLGFWETRGIPFEEVYLL
                     DVNEDVLPASRRGDSLLPPGVRRALGLPASRDKEREIEHYLDVLIGGARRVHLFFVEG
                     RDRERSRYVERLLWESRKRDPRKKSETPVGTVRYRAALGPYRPDEIPKTDETVRMLRE
                     LSYSATSLDAYLRCPLRFHYRYVLGLREREEIDEEIEAKDVGLLVHRVLEEEFAPLLG
                     RPLRPEDFPAGRLSSRVEELFRRAFGPNLSGRAYLMLLQTKRHLVDYINRCQVEVILA
                     CRAENKDIVILDLENRIRKASTIRRGAFTLSGRFDRVETRGGELYVLDYKTSADEKKF
                     GINWKTLDPDDRATWPKAVGSLQMPLYTVLSAALYERAEDRIQGRFVMLGKSRIGPGI
                     EVSPFDVPDGRRPVEPSVRAERIAAMGTIIDRLLAEIVDPGVPFKPAAEADRVCLYCD
                     FKGLCNRV"
     gene            complement(20954..22336)
                     /locus_tag="PKV64_02570"
     CDS             complement(20954..22336)
                     /locus_tag="PKV64_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15998.1"
                     /translation="MPRHRRGRVFAFLGVLAVLFFAGGSRPGAAAQLPELKTLEVFSD
                     NDCRITLGTFTVPPNQTGSSFVFSFQTPWQACQGPDRMDWITFGIRGAVSGEPLYECT
                     EYFDKRPALENPGPLDGITAGPGTYIVYLLSGRNSSVRLAYALSGETPPAAAPGEVKP
                     VTAYRDSSCNLFPGGFQVPPGCTASGFRFRLDSPWMQCSGPDRVDEITFTIRGPAGEI
                     YAYREYYDQRPAEERLGSLSGLVLNPGNYVVLLGSGRDTYLTLNYALNRPQPQAAAGP
                     GYKTAVLLAEIDTRFTSWYVKIWGTGNESVYRRGADSQASRTYSWTDISPEVISDTDI
                     TILAPGRIFIDAEFHMEDRPEYDAARVLYSVGSVYPQVWKNNAWQTPPEVYQANWQNY
                     IGYTGTRPIRPSKTFEYTGQMGGPIQILGTGLPVQPGKFRVSVLSAIAEDWKIHYPST
                     GKIYVYFIPD"
     gene            22497..23048
                     /locus_tag="PKV64_02575"
     CDS             22497..23048
                     /locus_tag="PKV64_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN15999.1"
                     /translation="MTKRASMCLSAIGAAAAGLAAVVFYSRGILPLTGVYVPKNEIGY
                     HLIYSAAAVAGAFLLAAALKRKKPVFWIGAGLLLLGIPLFFFAVSRPPFDYAPIRLRI
                     IGRSLIPAAFALLAVFALKGFRAAFGLLFAAFSALGLYHAVLTGKWIARGGPPADIAI
                     ALALAVISFFFAWAGLRALRRPS"
     gene            complement(23058..26627)
                     /locus_tag="PKV64_02580"
     CDS             complement(23058..26627)
                     /locus_tag="PKV64_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPN16000.1"
                     /translation="MFALHVSRRARDVYHFDESLFIFDGRVIFADIRSARLFAHKINA
                     ARPPDKAVRAGDLYALGLLEEYRHAVFQAYRDRVNPHVLRDALRDLSARRLDPETVRG
                     TLAFFLHEFPPPAVYKNRVPAGTYLDAPGFPPRREAALEELLMLRLTNLNPAAASFRE
                     LFDDDSLSRETAYLRILSGLREFFETQPPFGPERRNLIDVLNEPVLASPHSLKGQLEY
                     ILARWSAVLGRPLPRVVGGLDLLREEEKPGGGPGPSRAPGLGADSVEESLALFGGPAG
                     PRPDEPEPENFSPDVDWMSSLVLIAKNASVWLDQLSRKYGRSIRRLDEIPDEELDALS
                     EAGITGLWLIGLWERSPASRRIKQIMGNPEAAASAYALFDYAIAADLGGEPALLNLKE
                     RAGRRGVRLASDMVPNHMGIDSRWVIEHPDWFIGLPEPPFPSYTFNGPDLSWDDRVAI
                     RIEDRYYDRTDAAVVFQRLDKRTGSVRYIYHGNDGTSFPWNDTAQLDFLNPRVREAVL
                     QTILHVARLFPIIRFDAAMTLAKRHFQRLWYPEPGTGGAIPSRAGHGLSRKAFEAAFP
                     AEFWREVVDRTAVEAPGTLLLAEAFWLMEGYFVRTLGMHRVYNSAFMNMLRDEENARY
                     RRVIKDTLEFDPEILKRYVNFMNNPDERTAIDQFGDHDKYFGVCTLLAALPGLPMIGH
                     GQIEGFREKYGMEYRRAYYDETPREDLVARHRREIFPLFRKRALFAGVDLFRLYDFVR
                     PDGEVDENVLVFSNGTDGERALVVYHNRYAETRGWIRRSAAAAGKTVSLAEGLGLPDD
                     SSAWVVFKDAVSGLEYIRGAGELAREGLHLEPAAYSRAVYTGFRLVRDDERRLYARLA
                     AALGGRGTADVERAAVDLRLRPVQAAFRELANAGFFDWLMTKRIFRPGRPLDPEPGRQ
                     AGEKARAFAEAVARTIAEDEPRPRKAIDPEEIAAAARREFETILRLPVLEKRGRASGS
                     RTFQEALRFLKSALDGPAAPGKKPGRRRPGAKPHASRLKAGLDPRTAWISLFCGAVLR
                     TAGRTADKESPAGWIEAWGLDRIIDDAWRETGCAGEGRPNVPAIFQALEEYGGGIDPN
                     RSAAANARRLAGIITGDDRFRRALGLNRWQGIEYFHQEAFDALRWLLAAGAVCRATGS
                     GRGGVTAQAARAISRAFAVSDILRKAERKSGFETAKLRAALGA"
     gene            26737..28464
                     /locus_tag="PKV64_02585"
     CDS             26737..28464
                     /locus_tag="PKV64_02585"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPN16001.1"
                     /translation="MSQKIRSRIAGALAVFAAGAGLVLAGAVPPEPATLVLTNGKIVT
                     LDAAVPEAEALAARGDRIVAVGPAVEIARYIGPETEVVDLGGRLATPGWIDSHLHFMS
                     VGASKLSLDLTTAKSWEEIVGRVEAAVQNAVPGALITGRGWHQEKWNQVPRPNIDGLP
                     FHDALSRVSPDNPVILTHASGHSSLANAKAMELSGVTADTADPPGGRIIKDGEGRPIG
                     AFLETAMDLVQLGTSRARAARTPAEVMTENRKIIELADRECLSKGLTSVGDAGVGWTT
                     VELYKRIIDEGRLGIRLNVMLSENARRIEANAAAWKIEGYGAHHLSVHTIKRLIDGAL
                     GSHGAWLLEPYEDLPSSAGLNTESIEEMKETARVAVENGFQVATHAIGDRANRETLDI
                     YEEAFRAHPGKTDLRWRIEHAQHLHPADVPRFGKLGVVAAMQAVHCPSDGPWVPKRLG
                     AKRAGETSYVWRSLLKSGAVIANGTDAPVEAADPLPSFYAAVTRKMNNGEAFFPAQKM
                     SREEALRSYTVNGAYAAFEEGIKGTLAAGKLADVTVFTRDLLTCPEEEILSAEVAMTI
                     VGGKVLYKK"
     gene            complement(28483..29358)
                     /locus_tag="PKV64_02590"
     CDS             complement(28483..29358)
                     /locus_tag="PKV64_02590"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPN16002.1"
                     /translation="MFKKKLVLMILAAAAIAMFLPSGLTAGADLKIRVTVERANIRQS
                     GFASAPVIGGANKGEIFTVIEKAGDWYLIQLADGTPGFISAAVVEEVAETAPALPEEM
                     QPVERPAAPKPAPAALPDQVVGESGGKNTFSMVLLRAGYFLASDAAYKDVYENGLVFG
                     GEVRVGGQSLAGWVEGNYRSATGKTTYTEEETKMSVLAFEGGALYRFEAGMISPYFGA
                     GLGMYMFKESNEPMGEAKQSKLGFCGLGGVAVGLGSSFILDARLKYSTCSMKPADFDI
                     NVGGLTLGIGLGMKF"
     gene            complement(29369..31378)
                     /locus_tag="PKV64_02595"
     CDS             complement(29369..31378)
                     /locus_tag="PKV64_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPN16003.1"
                     /translation="MRKKTRRAAVHLLAALVLAAWPLAGLAQHEKNQAGIGQLREKAW
                     AEGRVQVIVHFTVPDIENLTADSTRFTGVDETPETALERSLADQVLAKTIEYASWRVL
                     TELQGTDFEFVTGFDYIPYITLRVSPNALAVLENSPNVLDISEDLPRKLIDPPGPGGE
                     GPKGGLSADEIRVDPLLDDTAALVGATAVWAMGYTGAGWYVAVLDTGIRKTHQFFTGK
                     TIIEACRAMGRDGLPGAGDCPNGLAVMNGPGSAVHYPSTYDGFDHGTHVAGIATGNYG
                     TLAGIAKGAGVIAVKIFSKFTAGDCGGSPCVSAWDSDILAGLNYVYSLRSAYKIASVN
                     MSLGGGLYPNFCDSVNSSVKSAIDLLRAAGIATAIATGNSYSCGRISWPACISTSVSV
                     GSSTKSDGDSSFNNWDSVTQRLYAPGSSIYSSTGDSDSSYASWNGTSMATPHVAGAWA
                     LLRQAFPTAGVTTLLTALRTKGKLIYSVCDGYNAGIPRIQIDKALQSLGSFILTIQRN
                     NTWGTTTPAPGAYAYPPNASVSVTAVPQNYVQFNGWTGDATGTTNPVTVVMNRNKTIK
                     AWYQYIYAPTASAKKVLNRSFSQAEYIDILTWQNSADNTGLTIASFKIYTVSGSTYTP
                     LTTVNADQTTYSRRNAGEAATQYAIAAITSAGREGAPALVTAELD"
     gene            31598..32095
                     /locus_tag="PKV64_02600"
     CDS             31598..32095
                     /locus_tag="PKV64_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPN16004.1"
                     /translation="MKTSETTNEDTVRRFRSLLRALERETIRPFDSRNRCCGLTLSQC
                     HALLEIGRRGEISLVDLAGVFGLDASTLSRTIQGLVQAGLVDRRPGERDRRYIAISLS
                     GKGQAAFRDIERRFDERVAAVLERIPAPRREAVVEAAALIAGAVRAENESGGCCPGRK
                     GNASR"
     gene            32082..33230
                     /gene="hgcA"
                     /locus_tag="PKV64_02605"
     CDS             32082..33230
                     /gene="hgcA"
                     /locus_tag="PKV64_02605"
                     /inference="COORDINATES: protein motif:HMM:NF040863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation corrinoid protein HgcA"
                     /protein_id="HPN16005.1"
                     /translation="MPLVKFPSAAGLEKESCCCGPDCCRGTKGGEKAGVFDRSIDPVR
                     FASGRRMTAVGEIPTVAARLGLRDRWGALRVRLGIGRGRYAIAPGLYALGSPDPWSPV
                     FVTANYKLSFDALRTKLPGIDAWILVLDTGGINVWCAAGKGTFGADEVAGRLEASGLK
                     KIVFHRTLILPQLGAPGASAPEIARRTGFRAVFGPVRAADLKAFLDAGMTAAPEMRRV
                     RFSLRDRLILVPMELAGVVRSRFFLAGLGLWLAAMIAGIRGAPLAGAAAAGAIFAGAA
                     ATPVLLPWIPGRMFAAKGAILGVLWASAVCLIGGFPSGGPADLAAAAAVLLFLPALSA
                     FLAMNFTGSSTFTSLSGVVAEMKVAVPVMLTAAGLGLAAAVLAAVIGG"
     gene            33242..33493
                     /gene="hgcB"
                     /locus_tag="PKV64_02610"
     CDS             33242..33493
                     /gene="hgcB"
                     /locus_tag="PKV64_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation ferredoxin HgcB"
                     /protein_id="HPN16006.1"
                     /translation="MPLRHLKNVTTLKYDPRKCAGCGLCAAVCPHGVFEMYGRTAAVT
                     GRDRCMECGACARNCAFGAISVRAGVGCASAVLWSMRRS"
     gene            34259..35035
                     /locus_tag="PKV64_02615"
     CDS             34259..35035
                     /locus_tag="PKV64_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015126930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPN16007.1"
                     /translation="MSFMKNKIVFVTGASSGVGRACARAFAREGASLLLAARRAARLK
                     ELAESLAAEFGAAVLPFALDVRDQAAVARAVGGLPENWKAVDVLVNNAGLSRGLDKFQ
                     DGLLSDWEEMIDTNVKGLLYVSRAVLPGMVTRGRGHVINIGSIAGHEIYPAGNVYCAS
                     KFAVKALSRGLRLDLCGTNLRVSTVDPGMVETEFSEVRFHGDKTRAANVYRGFTPLTA
                     EDVAEAVLFCATRPAHVNISEVIVMPTAQASTMLVHRRES"
     assembly_gap    35138..35237
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(35298..36761)
                     /locus_tag="PKV64_02620"
     CDS             complement(35298..36761)
                     /locus_tag="PKV64_02620"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPN16008.1"
                     /translation="MRFPPRRIAAVIVLAAVSGALSCAVNPVTGKQELMLVSEAKEIE
                     MGKATDESIRKEYGLYQDAALSAYLNTVARRMVPYTHRRALEYHFAVLDTPVENAFAA
                     PGGYIYVTRGLLAMMNSEAELATVVGHELGHVNARHSVREYSKQLLLTGGLVVGSLLS
                     RDVAKVAPFLMVGLQVLFLKFSRDDEYQADSLGVLYSRGAGYSPAQMIPFFRSIQKLE
                     EKAGGGMKLPNFLSTHPLTEKRIEEAEKMLEPQDAERNILRNELLAKMDGLIYGEDPR
                     QGYVEADAFYHPEMRFVFSIPAGWNVQNTPRQVVVASKDEKAGMILTAEETDLSPDLY
                     LGRRIEALENVRIQEVSRTNRMINGLRAARGTYLVSEEAGEGQAPPVTAVDFDCIAKN
                     GMIYTFTSMAAKADYPSFRNTFDRTVRSFANLSDPKRLAVQPKKLAVRRAGTAQTLKE
                     FLAGQGVPSDKWPTIAFLNALALEDKVEKGRLIKIMK"
     gene            37007..38689
                     /locus_tag="PKV64_02625"
     CDS             37007..38689
                     /locus_tag="PKV64_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HPN16009.1"
                     /translation="MFKGHPKGLFVAFFANMGERFGFYTMVAIFVLFMQAKYGMTAAT
                     AGFVYSMFMVCVYFFPLLGGLLADKVLGYGRTVSLGLIVMFLGYALLALPTRMNTGLS
                     LIIAAMAVIAVGTGLFKGNLQALVGSLYDDPKYDALRDRAFTIFYMGINIGAMFAPSA
                     SEIISNWVLKGAGLTYDARIPALANGFLKGTLTEAGAYLDIARTQDAGATLETLGTFS
                     ERYINALSKSYHFGFGVACLSLIVSMLVFWAFRKYYKHADFTEKQKAKSEALKSQVVE
                     LTPAQTKERLVALGLVFFVVIFFWMAFQQSGLTMTFFARDYTQLNVGALSYIWFDMIG
                     LLGIFLALGGLIFLFRKGSGKTARLAGGAAFLVFGALAAWRYAGYSAVNEFQPQRFQH
                     FNPFFIVFMTPVVVGFFSWLNKRNKEPSAPRKIGFGMLITAVSFAVLVIGSLGLPSPK
                     RLGGLVAPAESQVSVYWLISTYFIITIAELFLSPIGISYVSKVAPPKYKGLAQGAWFA
                     ATAAGLYLMGLVGGLWMRVPLWALWLILVAACLLSATFMFSVMKKLERASGQ"
     gene            38711..39187
                     /locus_tag="PKV64_02630"
     CDS             38711..39187
                     /locus_tag="PKV64_02630"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HPN16010.1"
                     /translation="MPISPFFNVVLIAPEIPQNTGNIARTCAAAGARLHLVRPLGFIL
                     SDRRLRRAGADYWSGLDLAVHESLEAFFRETPPERCLFFSQKAPRLFSEMPVEESVFL
                     VFGRESTGLPEEILAACRERSFRVPMRPGARSLNVSNAAAVVLYEALRRRGYSGLI"
     gene            39214..39627
                     /locus_tag="PKV64_02635"
     CDS             39214..39627
                     /locus_tag="PKV64_02635"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HPN16011.1"
                     /translation="MNIVRVPRLVKPGDLNHHGSLYAGRAAEWFVEACYLCAAQAPDL
                     PGTMVCLAIRDLVLSKPVRGGERIQIETRPVKAGRTSLTIYGKITTRSGPARAEGFVT
                     FVCVGKSGRPVAHRLRLDPPRDAEEKEIQRKARNF"
     gene            39773..40309
                     /locus_tag="PKV64_02640"
     CDS             39773..40309
                     /locus_tag="PKV64_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16012.1"
                     /translation="MKRNDFLKAVCGIGLAGRAAALNAGPAGRLKPEEQEKERDRFKE
                     AWLRTLMENMEKTLDPAVRERLMNDCGRECARRGVLMETAGKFQGDVAGFVRNLGPKV
                     GGDLCRVEGNIVHWGYPRCYCELVAAGPERLPDAYCLCSAGWVLEVFEAVAGRPVRVE
                     VVQTIKRGAPDCRFLVRL"
     gene            40416..41222
                     /locus_tag="PKV64_02645"
     CDS             40416..41222
                     /locus_tag="PKV64_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPN16013.1"
                     /translation="MVDWLRGLSPFLQALLATLLTWGATALGASLVFFFKRIKRSVLD
                     GLLGFAAGVMIAASFWSLLAPSIEMARRQGRIPWLPAAAGFLLGGLFLRLIDYFLPHL
                     HAGFEKVEGVKTNWNRTALLVLAITLHNIPEGLAVGVAFGAIGLGGGAATFGAAVALT
                     IGMCLQNFPEGMAVSVPLRRDGLSRSKSFFWGQFSGLMEPLAGVAGVLAVSRAEALLP
                     WALAFAAGAMIYVVVEEVIPESQVGEHTDIATLGTMAGFVLMMILDIGLG"
     gene            41270..>42768
                     /locus_tag="PKV64_02650"
     CDS             41270..>42768
                     /locus_tag="PKV64_02650"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HPN16014.1"
                     /translation="MMRSVVAALPPAEIDGFLAAIELFSDLTPAEREAVAARLKLVSY
                     EAGQALFEENQPRESLFLIYRGAVRLYKKSPLGEEKELARFRRFDFLGEGALMDDYPH
                     ATTARAEEPTLALVFSRTDVTGLLAAETSLAAKILSRTARVISRRMRQATNQVVNAAA
                     QYISGRTRREHDLLGERDVPAEFYYGVQTLRATENFPITGIAISRFPELIVALAQVKM
                     AAARANRDLGLLDAGVAAAIDGACREIIDGRMHTHFVVDMIQGGAGTSTNMNANEVIA
                     NRALELLGRERGDYAHCHPNNHINLSQSTNDVYPTALRLAFMASTCTLVAVLKKLIAS
                     FRAKGLEFKDVIKMGRTQLQDAVPMTLGQEFEAFAATLGEEVERLEQNAALFKEIHLG
                     GTAIGTGICAPADYAGRAVAHLSEISGVPLRLAPDLVEATQDTGAFIMFSSAVKRLAV
                     KLSKISSDLRLLSSGPRAGIHEINLPKRQPGSSIMPGKVNPVIPEVVNQ"
BASE COUNT         7372 a        13454 c        14134 g         7708 t
ORIGIN      
        1 cgtttcggtc gagaccttct cgaacgtcgc cttgtccgaa tcggcctcgc agccgtaagc
       61 cccggcggcc gttcccatga tcgagaagtg ggacaggtcg aacgggtatt ccttctccga
      121 gccggcggtg atgatcccga acggctgggg ataaaggacc tcggccgccc ggtaggcgga
      181 tttcccgatt tcgcacatcc cgatgcagcc gtccacgcac gtgacgcaca tcccgctgaa
      241 ggggctgatc gacccttccg tccggttctt cgtcagcgtc gccgcgctcc gattgagtct
      301 ggtgaatgac atgaccggtg acctccttta gatgatctcc gtcaacgctt cttttcctgg
      361 acgcaggagc cgcatcgtcc gacgtcgtac atccagcaat tcagctcgtc gtattgttcg
      421 aggcaggtgg gctcgatctg caggcaggcg ttgcagatgt cggtgtcggc gttcgggccg
      481 ccgcaccggg cggagcaggc cggacagacg tagaggcagc cgccgcacag ccggcactcc
      541 tccgagcgga tgtcgaacgg cgtcgagatt ttgcgcttgt agccccggtt ctggaaaccg
      601 atggcccggg cgtccatctg ctcgtcgcac atccggatgc agaggccgca gtagacgcat
      661 tcctcgttgc ggggcgggaa gcgctgctgg gtgacgccga acttggaggc caggtcctgg
      721 atcttcttgg acatgggcgc cgtcgagagc atgagctcga tcatcacccg ccgggcttcg
      781 atcacccgcc gggtgtcggt gcggacggcc aggccctttt cgacggggta ggtgcaggac
      841 gagacgagtc gggatttcgg cggttctccg atttcaacca ggcagaggcg gcagccgccc
      901 cacggcgtga gaccctccat ccagcagagg gtcgggatct ccaggccgta gaagcgggcc
      961 gcctccagga cggtccagtg ctcctccgcc tgaacttcca agccgttcat gaggattggg
     1021 atcatggctt ctccttcatc gccttaaggg cggccttgcc gtcctcggtc tccaggtcgc
     1081 agcggaggca gcggcgggct tcccggacgg ccgcccgctc ggtcaagccc agctccactt
     1141 cccggaaatt attcctccgg tccgtggtcg acagccgatt catcgccggg cggcgggctt
     1201 tctgaatctc ctcgtcggac aaggccggag gggcggcgta gagggacggc cgggacagct
     1261 tgtaatcagg cttgagggcc ttgccctcga cgtacttggc gatcatctcg gccgcccgct
     1321 tgcccgcggc catggcgtcg acgaccatgt tcgggccggt cacggcgtcg ccgccggcga
     1381 acaccccggc ccgggtggtc atggccgttt ccgcgtcgat gacgatgttt tctccgtggc
     1441 gggtgatctc gtcgccttcg ccgatgtacg acgtgtcggg ccgttcactg atggccagga
     1501 tcagggtgtc gagcttgtga acgaaattcg agccctcaac cggcaccggc cgcggccggc
     1561 cgctcgcgtc gggctcgccc agcttcatct tttggcattc gatcccgacc gctttcccga
     1621 agctcatcag gaccttgacc ggggtgacga gaaagcggat gtccacccct tcctcgacgg
     1681 ccgcctcgac gtcctcggcg atggccggca tctcggcctt ggtccggcgg tagaagatgg
     1741 ttactttttc gcaatcggga tcgcgcaagg cgacccgggc ggcgtcgacg gccgaatttc
     1801 cgccgccgac gacgaccacc cgttggccga tcgggaccgt tttccccatg ttcacctggc
     1861 gaagatactc cagggaatga agaacgccct cggcgtcctc cccggggatg cccatccgga
     1921 gggatttccc cgccccggtc gcgcagtaaa cggccttgta gccggactta aagagcccgt
     1981 cgatggtgat gtcccggccg agggtcttgt tcaggcggat ctcgacgccg gcgttcttga
     2041 tgtcggcgat ttccctctgg acgagcggcc cggggaggcg atggtcgggg attccgacgg
     2101 ccatcatgcc gccggcgacg gacaaggcct cgaaaatcgt cggccggaat cccttcaggg
     2161 ccaggtaata accggccgtg aggccggccg gccccgagcc gatgatggcg atcttctccc
     2221 ggttctgttc gatcttcggc gggacgtaac ggattccgtt ttttcccgcc cagtcggcga
     2281 cgaagcgctt gaggccgcgg attgagatcg gctcgccgga ctcgccggcc cggcaggcga
     2341 gctcgcaggg gtggtggcag acgcgggcgc agaccgaggg aaggggattg tccttcatga
     2401 tcacccgtaa agcttcttcg taccggccgg aagcgatgag ggcgatgtac gtcgaggctt
     2461 cttggccgat gggacagacg tactggcagg gggaggagac gatttccttg cagacctggg
     2521 ccggacattt cttccgccgg atgtgctggc ggtattcgtc cttgaagtaa cggagggtgg
     2581 acaggaccgg attggcggcc gtctggccca gcccgcacat cgaagcgtcc cggcaggccg
     2641 cggccaggtc ctccagggtg ccgatatcct cctcccggcc ttggccttcg gcgatccggg
     2701 acacgatttc ccacatcctc tgcgttccct cgcggcagga gacgcatttg ccgcaggact
     2761 cgtcgcggag gaagttgaga aaataccggg ccaggtcgac catgcacgtc ttctcgtcca
     2821 tgacgatcat gccgcccgag cccatgatcg acccggccgc ggccaggccc tcgtaatcga
     2881 tcggcaggtc gagcttgccg gccgggaggc agccgcccga agggccgccc gtctggacgg
     2941 ccttgaaccg ccggcccccg gggatgccgc ccccgatgtc gtagatgatt tccttgaggg
     3001 cgaggcccat cgggacttcg accaggccgg tgttgttgat ctttccgacg agggagaaga
     3061 tcttcgtgcc cttgctgccc ggcgtcccga ccttggcgaa ggccgccccg ccgccggaga
     3121 tgatgatcgg cacgttgccc caagtctcga cattgttgat gaccgtgggc ttgccgttga
     3181 gcccccggac cgagggatac ggcgggcgct ggcggggctc gcccgtccgg ccttcgatcg
     3241 aggcgatcat ggccgtttcc tcgccgcaga cgaaagcgcc cgcccccttg acgatccgga
     3301 aatcaaagga gaagccggcg cccaggatgt ccgggccgag gaggcggcgc ttgcggaggg
     3361 cctcgatggc gatggtcaca tgcttgacgg ccaagggata ctcgtcccgg acgtagatga
     3421 cgccctccgt cgccccgatg gcgaacgccc cgatcatgat gccttcgagg acccggtgcg
     3481 gattgccctc caagaggctg cggtccatgt aagccccggg atcgccctcg tcggcgttgc
     3541 agatgacgta tttgacggag ccgggctgtt tgcgggtcag gtcccacttc agggcggtgg
     3601 ggaagccggc cccgccgcgg cccctcaggc cggaggcctt gatctcggcg atgatgtctt
     3661 ccggaccgag cttcctcagg accttggcca gggcggagta accgccgatg gaaaagtaat
     3721 cgtcgatcga ggtgggatcg atccgggcgt tgtcgcccag gaccagccgc ttttgcttgg
     3781 cgtaaaaggg gatgtctctt tcccgtttcg ccggcttccc ggtggcggga tgatggaaga
     3841 acagctccgg gaccggtttt ttcttccgga tggtttcgga gacgacgagg gccgcgtcct
     3901 tcggatcgac cttctggtag aacaggtcgt ggggttggac gatgatgttc ggctcggcct
     3961 cgcagtaccc gtggcagccg gtgacccgga ggcggacttt cttgtcgagc ttggccttcc
     4021 ggagggaagt ccggagagcg tcgacgacct tgagggctcc ccgggcccgg ccgcaggtgc
     4081 cggccgaaac ggtcaggata ggcctccggt cttttttccg aagccgctcg tgggcggcga
     4141 tcgtctgttc caagacagcg agcgttttga tcgggacgac tctcatgagg ctcacttctt
     4201 cccgtactgc ttgatgatgg ccgcgacctt gcgggtcgac gtctgggcat ggaagtcgcc
     4261 gtcgacgacc acgaccggtc cgatcgcgca gcatccgagg caggccacgg tttcgagggt
     4321 gaacttgcgg tcgggcgttg tttcgcccgg accgacgccg agctgccgct cgaattcctc
     4381 caggatcttg ggcccgcccc ggacgtggca ggccgttccc gagcacaccg tcacgacgtg
     4441 gcggccgcgc ggcttcaggc tgaaagcccg gaaaaacgtg gccaccccga cgacgtcgat
     4501 gagcgggaga ccgacggagc gggccacctg ccggagggcc tcctggggca ggaagctgta
     4561 ctccgcctgg acgtcctgga ggatcgcgat cagggccttt ttctggttct ggtgcttccg
     4621 gataaaggcg ttcacgcggt caatttggac atccacgcgt caaacctcct tcggagtttt
     4681 tgctagtcag gaatgaaaat tatgaaaggg agagaaggag cggaaagatc aggcgagggg
     4741 atggtctttc cccggtgaag gggcggtttt ctcgaggccg ggagccgaat tgtcaaagac
     4801 atgactcctc gaaagccgat agagaatgcg tctctcatat cacacgaaaa accggttgtc
     4861 aagagaatat tatgaaaata atagattgaa ataaaaacga caagtgtcgt actatgacaa
     4921 gaggaaacgg ccgcgaaaag gaggatgtca tgagacacga gatcctgtcg gatgaaagcc
     4981 tggaaatcat gcgggaaata tgggcccggc cggaagcctc gatcaacgac gtctggaccg
     5041 ccctcaacgc cgcccggcgg aagaaggtcc gccggaccac cgtccaggtc caaatgaccc
     5101 gcctcgtgcg gtacggctgg ctcagccgga gaaaatccgg ccgggaatac ctctatgccg
     5161 ccctgtacgg caaggacgaa acggtcggcg accttctcag cgcgctggcc cggcgcgtct
     5221 acggcggatc gttcgtcgag ctggtcgggt tgctcctgga gcgggggatt ttcagccccg
     5281 cggaccggcg cagaatcaat gtcatgtggc ggaaaataag acggggaaag gaaaccgggc
     5341 cgcggctttt ccctgcttga aaaacggctt ggactccggg actcagagga ggcgccaggc
     5401 gtggaaatcc tcaaacaagg tccacgtccg gtaggctgga tccgggcttc cgtccgggaa
     5461 gaccggggcg aatcgcgcgg aaagcgatga agggacaggg cgggggacga acatcgtcca
     5521 gaaggcgagg cgtcccccgg accgggaaac gcgaaggatt ccgccgagcg cccttgcgta
     5581 ctccccggcc gccatgtatt caaagatgtt ggagagattg aatttcgaga aatcgccggg
     5641 atgggtcgcg gcgaggattt catccaggcc ggccgtccga agcttcaacc ggtccaatcc
     5701 ctcccggatc gccgggaaat gagcggcttg cagccatagc ggaagattct ccggccgggg
     5761 gtaggcgctc tccttgaaaa aataagcgag gccgggatga tcccggacga gacgttggct
     5821 gagctcccgc tccgcccggt cgtagaaaag ccgccaggtg tcggccgcgg tcacgtagcg
     5881 gtagtgggac cgttgacgac cccaccggcc gaacacgcgt tttccgaaca ccaggcggaa
     5941 cagcgtccgc caccgccgat tggtccagac gtcccggtaa aacgccgcct gctccccgac
     6001 cgtggggcag tcgagcaagg ctcgcagcgt cttgcgggaa tggatcaagg gaacgacata
     6061 acggatcgcc gcccggaaga aacgctcgta ccggccgcag tgaaacaggc cgtcccggat
     6121 caggtcccgt cggcggtccc aaaaagcccg ggccggcccg ctcaagcccg gcctcaggac
     6181 gtcgtataaa cggtccttgt ccggtccggg ccgggcgccg agaaagccca gaagctcttc
     6241 gcggtcgagc cgccgggcgg ccaggacctt cagctcgacc aggtgtatct gggccggatt
     6301 ccggtcgatc aaggtcaccg agcgcggccg atcgagaagg aaagccaggc cgttttctcc
     6361 cccggaagcg atggacagga catcgtcgtc cggtccgacg gcaagcgcct cccggctgag
     6421 acggggatcc tcccagcatt gggcgtaccg gacctccagg cggctcatgc ctcccgctcg
     6481 tacagggtat attctcgctg cggttcgatc ttcagccggc ggaagaacgc ttggatgggc
     6541 tgatccttgg gcatttgggc gaagatgaag tccgaataac cgcggcggag cgactcgtcg
     6601 atcatggcga agaatcccgc tcttcccacc ccggtccccc gcgcttcttc ttccggaagg
     6661 gaaccggccg cgaagaaatt gaagcgtcgg cgcttccgga ggcgggacag gaatcggagc
     6721 gcgccaaggg gcgtgatccg gccgttcatg gcgcggaccg cgccggccag gtcgcggaag
     6781 atgacgccga agctgaccgc ccgcccggct tgatcataaa cgaaacccga aacgtccgcg
     6841 gccagggcga atcccacgcc ggaaagaagc gctttgaact cggtgaacgg aatcggcgtg
     6901 taatcgggaa aaccgtcgaa cgaccggtcg atcaggccgt gcagcgcggg ccattccttc
     6961 cggaggtcca ggcgcgtcgc cgggacgaac cggtaacccc gctcgaggag gagctcatgc
     7021 cttttcctcc acggcgcgat caaggcctcc agcttttccc ggccgttgat ctcgatcgaa
     7081 ttccacgccc gaagcggcgc gaagccgccg agctcgaatt gttccgggta atacgccggg
     7141 ttgtcgggct cgccgtagta cggttcgcgg tcgaatccct tggtcaggaa ccggtagccg
     7201 tgccagaagt cgaaattcat gggtccccgg atccgccgga cgccgtgttc cgtccggagc
     7261 cactcccggg ccgcctccaa aagctcggcc gcgacgccgt cgtcgtccac gcattcatag
     7321 aagccgacga ggccgacggg gagccggtcc ccgtcccgga tctccgcgtt gaccatggcc
     7381 gcgatccggc cggcgacgcg gccggcccga taggcaagga aacggcgatg atcgtttccg
     7441 gcccgttgat aaaaatagaa atcggggctc aactggcgga tgatctcccg gcggaagggc
     7501 gggacgcgcc gggcgccgcc ggcgtaaagc gagccgccga aagcgatgaa ttcccgggtc
     7561 agggacggat cgaaccgaaa cgagcgaagg gacatgattc aggaatcact tccgcccggg
     7621 cggctgatgt ccgcgtgttt gtttctcatg gggaggatga ttttgccaga ttcagccgga
     7681 gcgaatcaag gcgcggggcg aggattcgaa gcggccgcgg cccgcccgat cgaatctccg
     7741 aggcggacat atgtttccat tccttcggcc gtgcgggcga gaaggtcggc gtcgacctcg
     7801 atcatccctt tccggaaaag aagggcgacc gtcgacccgc ccggctcgaa acgcgctttc
     7861 ttgtcaccct tggccgccgg gacgccgggc cggaaaactt ggcggatcga gcccacggcg
     7921 aaggctccga cctcgatgat cgccgcccgg ccgaaacgtt ccgtctcgaa cagggtgatc
     7981 atccggtggt tttcccggaa ataagggacc gtccgtttca gggaataggg tcccgagggc
     8041 cggtattttc cggggagcga aatcgccggg gcgggaatcc cggaacaggg gaaatggacg
     8101 tagtggtaat cccccattcc cagccggctg acgaccagcg ttcccccggc gaagtcctcg
     8161 gccaggcggt cgtcccgaag aaagtcccgg aggttgaaaa gggcccgttt gacgggaaaa
     8221 accctgtcga ccgggagatc cgggtaggcg agaatccttc cgtcggcggg cgcggcgcag
     8281 gcttcggacc cgggaacgaa cggacgacgg gccgggtcga tctcgcggac gaagaaatcg
     8341 cgcaggctcg cgtattcccg ggccggcttg gccagatcgg cgggatcgac gccgtacgtt
     8401 ctcatgaacg ggccgatccg ccgccggctc aggcggcttt tctggaaccg gccgtagaga
     8461 cgtgaggcgg ccgccccccc gaaaaggacc tccccggccc ggcgcccgag ccgcgtgttg
     8521 tggatccagt gggtcagccg ggcggccgga acggcgtccg atttgacccg tccctcccgg
     8581 cggtcgtagt attcaagacg atcggcgtcg ctcatggatg atgatccgcg gctccccatt
     8641 ttactcgaac ggccggtccg gaaaaagtgc gcggtgccga ggggcgattc ggatggtata
     8701 atggcgggcc tggacgcgcg gcgggtgctg gagaaacagc gtttcccgcc ggggaggaac
     8761 acgaacgatg atcagccgga tctggcacgg gtggacgacg cgggataacg ccgatcaata
     8821 cgaaaaactg cttaaggaag agattttcga gggtattctc aaccggaaca tccggggctt
     8881 caaaagcatt cagctgctcc ggcgtgatct tggccgggaa atcgaattcg tcacgatcat
     8941 gacgtttgat tcgctggacg cggtccgcga attcgccggc gaagactacg aagccgccgt
     9001 cgttccgaaa agcgcgagag ccgtcctgtc gcatttcgac gaccgttctc ggcattacga
     9061 aatcatcatt gagcgaaccg gagagaaata gccggcggcc tttgaccgcc cgcgcgaaat
     9121 aatcggagga aggtgcattg atgcgagtgt cccgaaaaac cgtttttacg tccggatccg
     9181 tcgccctgat cctttggctt tgcctggcct tcgccttcgc ccaagctccg gccaagcggc
     9241 cgcttgcgat cgacgatgtc ttgggcggac tggagatttc cgatccgcag gtgtcgccgg
     9301 acgggggatg gatcgcttat accgttgcct ggatggaccg ccaggccgac aaagcgcgaa
     9361 gcgccgtctg gatggtcgac tgggagggcc ggaacaacgt cccgatgacc tcggggccgg
     9421 agggggacac cgcccctcgc tggagcccgg acgggaaata cttgtccttt ctctccaccc
     9481 gtccggccga cggcaagacc caggtctggc tgctcgaccg gcgcggcggc gaggcccgcc
     9541 ccttgacgga cgtcaagggc gagctcagcg cctatgaatg gtcgccggac gggaagcggc
     9601 tcgtgctggt catgcaggaa ggagaagaaa tcccggacga ggcggcggat tcgaaagcca
     9661 agacggcgaa gccgatcgtc atcgaccggt atgccttcaa gcgggactgg gtgggttatc
     9721 tcacggcctc ccaccgggat cgtctctacc tgttcgacgt cgggacgaaa acgctggccg
     9781 ccctgactgc gaaaacaaat catgacgaga gtgatccggc ctggtcccca gacggatcgc
     9841 ggatcgcctt cgtccggggc cacggggagg attcggaccg gaccgggatg agcgacgtct
     9901 tcatcgtcga ggcccgggcc ggagccgagc cgcgccaagt ggcgacgatc ggcgcgccgg
     9961 acaggcagcg gctggtctgg agccccgacg gacgaacgct ggccttcacc cggggattcg
    10021 agccgaagta ctatgcctac aaccaggatc ggttggccgt cgtgccggtc gaaggcggcg
    10081 aaccgcgcgt tttgaccgcc gagctcgacc gcggcgtctc ccaggcggaa ttcactccgg
    10141 acggctcggc cctgaccttc ctcgtggagg acgatcagcg gacatacgcg gctgaagttc
    10201 cggccgtcgg cggagccgtc aagcgggtga gcggggggat cgaagtcatc agcgagatcg
    10261 cccgcggagg cgggcggacc gcggtcgcgg ccgcgaccga cgccgcggct acggaaatct
    10321 acgccttcga aaacggcgcc ctccgcaagc tgaccgccca caacgacgcg ttgttcgccg
    10381 ggctgaagct cggggccgtc gaggacctga gcttcaagag caaggacggc acggccatcc
    10441 gcgggctgat ggtcaagccg ccggattacg agcccgggga aaaatacccg acgctcctct
    10501 ggatccacgg cggcccgaat atgcaggacg accattccct gacgttcggc ctgtatccgc
    10561 tgcagctgga gcggcagctg ttcgccgccc ggggttatgt cgtcttggcg gttaactacc
    10621 gcggcggaag cggttttgga gcggctttca cccggagcat ttttgccgac tggggcgcca
    10681 aggaagtgga cgacttgctg gccggcgcgg acgaagccgt gcggatgggg gtcgccgacc
    10741 cggaccggct cggaatcggc ggctggagct acggcggaat cctgaccaac tacacgatcg
    10801 ccgcggacgg ccgcttcaag gcggccgtca gcggagccgg aagctcgaac caaatcgcga
    10861 tgtacggcgt cgaccagtac acgatgcaat acgacaacga gctcgggccg ccgtggcgcg
    10921 ctccggaagc ctgggcgaag gtgtccaccc cgttcctgcg ggccgaccgg atccacacgc
    10981 ccaccctgtt catggggggg gaaaaggact tcaacgtccc gatcagcggc agcgaacaga
    11041 tgtaccaggc gctgcggacg ctcggcgtcc ccacccaatt ggtcgtctac ccggggcagt
    11101 atcatctgct gaccaagcca agctacatcc gcgaccggct ggagcggacc atggcctggt
    11161 tcgacgcgta tttgaaagcc ccgcaaaaat agagccggcg tcccgaggag cgtccttccc
    11221 ggccgcgacg gtcagaaccg ggctaagacg gcggccgcga gcaaggccaa gccgtagagc
    11281 gccgcgaacg ctccgtggga cagccgcccc gcttttccga acgcccgcag gaccgggctt
    11341 ttccggacga tcaggccgat tgagacgacg gcgccggcca aggcaagcac agccaagatg
    11401 ctcacggcgg gggcgacggc ggcggcgagc cggttgtcca ttttcaggct tccggcggcg
    11461 atcgcccggg cggtttccag gccggcgggg tcgcggaagg ccggggcgta ataaggaaga
    11521 cgcgaagcga tccggatgag ggctttgacc aggtggccga gcgccgtcac gggaaggagg
    11581 aggaggccga acgtccgggc ggactccagg agagtcgttt tgttcaacat ccgtccggcc
    11641 gcggccggga tgagaagcag caaggcgggg aaaagaagaa agagaatcac ggcctggacg
    11701 aagttggccg ccccgcccga aagcccgagg gccgcgttgg ccgcggccgg aacggcctca
    11761 agagccttgc gggccggacc ccattcctcg gccagctccc aaacgaccag cccggagacg
    11821 gggacgagca ggaacaggtc cgtgttgcga agccgcaggc ccgtgaaaaa gtcggacagg
    11881 ggcttgcgca atgacagccg aagattcctc gacgggcagg ccttcaggca ttcggtgcag
    11941 acgaggcaat cgcgattatc ccggatcgcg gccggatgaa gcccgctcgg gcaggttgcc
    12001 gccgggccga agccggcctc cggccgggaa aggcagtttt tccccccgca agccgcgcag
    12061 atcccgggat cccggacgcg ccattccagg gccgccccgc aggcgtataa gcccaggatc
    12121 cggccgacgg ggcaaaggtg cgagcagaac gcccgccggt tgaagagaaa tccagccgcc
    12181 gcggcggagc ccaggagaaa cagaaaaaac agggccatgc gctcgggata gcggtgcgcc
    12241 caaaatgtcc ggatgaatcc caggagggcg aggatataaa agaatacgac gccccggccc
    12301 gaggcgagaa accgcggaat ccgctttttc aagccgatcc gggaacacgc cgaggcgacc
    12361 atttccatgg ggcagaccgt gcaccaaacc cgtcccagga cggccgcgct catgatgacc
    12421 agcggccacc agagcgacca gacgacgagc gaggccaggc tggtgttgcg caggattccg
    12481 gtcaggccgg gggagacgtg agggacggcg agccccccgg cgatgagaag gaggaaagcg
    12541 gcgagggtga aaagacgggc ggcgcttggg aaacggggcg aggccgccag ccgggcgatg
    12601 aaaccgctgc ggagaaaatc ccgacccatg acccgcctcg tcagattttc gacagggcgt
    12661 cgcgggccgc ttcccggaag ccttccgaga ggctcggatg gtgataaatc gagtagacga
    12721 aatcctcggc cgtcgccccg gtccgcatgg ccacgacgag attgtgaatg atctcggccg
    12781 attccgggcc gaggatatgg gcgccgatca gcttgtggga ggacgggtcg aagagcatct
    12841 tgataaagcc cggattgaag gtctggaggc tgcaggcctt gaccagggag gcataggggc
    12901 aaaagccgac gatgagcggg atccccttgg ccgcggcctc gtcctcggtc aggccgaccg
    12961 aaccgatctc gggctgggtg tagatcccgc cggggacgaa gcgctcgtcg atcgtccggg
    13021 agccgcccaa ggcgttttcc gcggccaccg cgccctcgag gatggctgtg gaggattgca
    13081 tcctccggcc gaggacgtcg ccggcggcga agatgcccgt tccggtcgtt tccagacatt
    13141 cgtcgacgat gatttctccc cgcccgccga cggcgacgcc ggccgcctcg agcccgaggt
    13201 cttccgtcga gggacgacgg ccgacgcagg tcaagaccag gtcggcggcg aatgtttctc
    13261 cgctctcggt cgtcacgagg cgtccgccct cgagaccggc ggcgcaggcg gagaccgacg
    13321 tcccgaaacg gagggcgact ccgcggcgct tcaattcctt ttcgacggcc gcgacgacgt
    13381 ccttgtcctg tccgggaagc gcccggccgg ttttctcgat cagggtcacg tccgctccga
    13441 atgtccggaa gatgaaggca tactcgcagc cgatgatccc ggctccgatg atgacgatcc
    13501 ggggcgggca agaggccagg gcgacgatct cgtcggtggt gatgaccgta gcgccgtcga
    13561 cgggcaggac gggcagatga atcggatggg agccggcggc gacgatggtc cgggagcttt
    13621 cgatcacggc ttccgttccg tcggcccggg cgacccggat ccgatcgggg gcggcgaacg
    13681 cggcccggcc ggacacgagc gtgaccttcc gcgaccggag atgcgattgg atgacgccga
    13741 tgtcgcattt catcagccgg ttgcggctgg cgacgacttc ctggaagtcg agggacaccg
    13801 gtccgtcgat ccggacgccg aagcttttgg ctttcttgat ggtttcgaac atcccggtcg
    13861 aaatcgagaa cgtcttgctg ggcagcgtcc cggtggccac gcaggccccg cccagctggg
    13921 agctgctttc gatcaggccg acccgggcgc ccagctcggc cgccttgacc gcggccgcga
    13981 gcccggcttc cccccctccg atcacgaccg cgtcgaagcg caaggtttcc atgcgcctat
    14041 tctactcgaa gccgccggcg cgtccaagcg cggctcggcc cgttgacagc cggagcgggg
    14101 gcttttatag tgggcggagg attgcatcgc ttccggaaaa aagaaggaga cgccgccatg
    14161 tccgcctgcc gcctcgcctc gctgaaccgc cgggccgccc aaggggttct cctcggactg
    14221 atcctttccc tcacctccgc cctggcccaa ttgcccccgg cttccccggc cgaagccggc
    14281 ctctcggccg agcggctgga acgcttgtcc ctttacctgc agccggttgt ggatgaacag
    14341 aaagcggccg gccggggacg atcgggtcgt acggctgggg aggcgcgtac cacacgacct
    14401 attgggtcga tccccgggag cagatggtct gcgtcctgat gacccagctt ctgccggcga
    14461 gcggcagcga cctccacggc aagttccgct ccctggtcta ccaagccgtc atcgaaagcg
    14521 gcgataaata acggacgctt ttttccccgc gccgccggag gcgggtcaag gtctttccgg
    14581 cccggcggat cggcgaagcg ctcaacttga tcttccccgc tcctctcccg gctcaatctt
    14641 cggggaactc gccggcaaga gggggagcgg attcgccgat catagggacc ggatcgagcc
    14701 gttgacgggg aagcggcggc atggtaggtt ggccggagat gggatccgaa ttcaaactga
    14761 cgcgggcttc ggccgggtcg ggaaagacct atgccctgtc cctaaggttt gcccggtttc
    14821 tcctgacgga cggtcctccc ggcgtgcccc gggacctccc tcacattctg gccatcacct
    14881 tcactcggaa cgccgcccgg gagatgaagg aacggatcct cgactggctc aaggaagggt
    14941 atcggggcga gggaaagaaa gccgaagcgc tcagggtcaa gcttcaccag gagtttcacg
    15001 aacgtctctt cagccccgtc gaactgagcc ggcgctcgga ggcggcggtc gagcggatct
    15061 tggccggata caccgatttc caggtggaca ccatcgacag cttcgcggcc atggttttcc
    15121 gggcctcggc cgtcgacctg ggctacggcc ccgatttcga gatctccctc gatcccggcg
    15181 agcttgtcga ctacgccttc gcccggtttc tccgccgggt caggccggaa agcgccgagg
    15241 gcgaggtctt ccgccggatt cttgattatc ttcttgtcca ggaaagcgaa aaagcgcggt
    15301 ttctttggga cccggccccc aagatcctgg aacggctgaa ggcgtttcag aaaaagctga
    15361 cagcccggcc cggcgagctc gtcctcgacg atttccagga ggaaaaagcg aagctcggga
    15421 aaacgatccg ggggacggcc ggccggctgc ggggggccgt ggaagagtcg ggcctggatc
    15481 tctccgcccg cggccactac cgcggccgga tcatgccggc gatcgaggac gggcggttcg
    15541 ccgatctcct tgattgctca ttcggcacgc tcccggttaa gggcgggagc aaggccaagg
    15601 gagccgcggc cgaggccctc gcgaggatcg agaaggaatg gaaggccctc gaggggcttg
    15661 tcggcgaata cgcccgcctg cacgcccgcg atttttttct gccctacctg cgggcttatc
    15721 aatcggttca agcggaaatg aaggcggcca agctcggccg gggccttctc ttcctcgacg
    15781 atgtaaaccg ggagcttctc cattacctcg agcgggggat cgtcccggac gtctatttcc
    15841 ggctgggcga ccgcatctat cattacctga tcgatgagtt ccaggacacg tcgcccattc
    15901 aatggcggat tctcaagccg ctgatcgagg aatcgctgag ccggggggga agcctcttga
    15961 tcgtcggcga cgccaagcag gcgatctacg gcttccgcga cgccgatttc cggatcatgc
    16021 gggacctgga ggagggcgcg gacggcttcg cctcggcccc ggtatccccg gacgatctgg
    16081 agatgaacta ccggagcgag ggccttatcc tggattttgt caaagaggtg ttcctcggct
    16141 tggcttccgg gtccggcggg gaaggggaaa aaagcggcgt cgattacggg cgcttggccg
    16201 gattgtccgg gctcgatcat ttcacccagt ccgttcttcc ggagcgcgag gggcgcggat
    16261 atgtccgtta tatcctgttc gacaaaggcg gggcggccgc cgaaaccggg gaaggggagg
    16321 aggagaactc cggggagccc ggcggcgatt ccgaagcccc cgaaaagagc gagatccaga
    16381 cgctgatcaa ggagctcttg ggccgcggct ggcgacgggc cgacttggcc gtcctggccg
    16441 gccgcaacaa tgaagtcgcg gccgtcgcct cctggctcaa cgagatcgac atccccttcg
    16501 tctcctacag cagcctcgac atccgccgcc ggaagatcat cggcgagatc ctctcctttc
    16561 tgcgcttcct cgactctcct cccgataacc tggctttcgc tgagtttctc ctcggcgatc
    16621 tcttcacgaa ggtcgcgggg aaggaattcg atctttccgc ccggcgcgaa ttcctccaaa
    16681 gctgcgcccg aggcgacggc gggccgcgct atgccgctct ccgccgggtc cggccgggcc
    16741 tttgggagga gtatttcgag ccgttcttca aaagcgttgg ctatctgccg ctttacgacc
    16801 tggtgacacg gatgtaccgg caattcgacg tgttcgggct cttccccggc gaggaagccg
    16861 ccctgtccaa gctcctggag gcgatcaaga ctttcgaggg cgaaggacgg aacgaccttc
    16921 gcgaattcct cgccttcagc gcggacgagg aggattcgga cgaaaattgg acgatcgacg
    16981 tcccccggga aatggacgcg gtcaagatca tgagcatcca taaggccaag ggggccacca
    17041 tcccggtcgt gatctgcctg ttttacggtc agtccttcag gggcgaggaa ttctatctcc
    17101 atgacgagga ggacggcgtt catgtcctca aaatcaataa aaagctggcc gagagcgaag
    17161 cgtcgctgag tgccgcgtac ggggaggcct tggaccggga gtgggtggac cggctgaaca
    17221 cgctttatgt cgccctgacc cgggcccaat atgagttgta catcgtcggg gtcaagggca
    17281 aaaaaggaaa atttcctttc gatctcctgg aacgggcgtt tccttcctcg acggaagagc
    17341 cggtcgtcca cgcggatgga acccgtttga accggagctt ccgggaaggc gaaccggccg
    17401 ggcccgagtc ttatcttcga gccaagggac gggatgagga agccggggaa tccggactca
    17461 ttcgggaaat cacgcaccgg gccggtttca ccgagccgcc ggtgaacatg cggacctcgt
    17521 tgaagtccgc cggcatccgg cgcggcgaat ggatccaccg tctcctggcc ggagtcgagc
    17581 tcaaccgggc gggatgggac gcggaggtcg ccgcggcctt ggaaggcctg ggcctctccc
    17641 cggccgagcg gacggcggcc ggggaagccg cggatgtcct gcggcgggcg ttcgggaaat
    17701 ccatcgccgc cgattggttt gaaccccgcc cggaccgctt catccttcgg gaggccgaca
    17761 tctgcgatcc ggaaggcgac gttttccgga tggaccgcgt cgtcctcgac cccggcttgg
    17821 tcttggtcat cgactttaaa accggcggcc ggacggagga agcggaagcc taccgggagc
    17881 agatccgccg ctatgtccgg ctgctgaggg atgtccatcc cggccgcccc gtccggggtt
    17941 tcctgtcgtt tgtcgaccgg gatcggatcg aggaggtgga atgagcgtcg gacggattcg
    18001 gctggtgccc ccgcgggaga gcctgatcga ggccgtggcc ggccttctgc cttccggcgg
    18061 ccgggatctt tcccggtctt ggatcgtctt cccggaacgc cggccggcgt attacctccg
    18121 caagcatctg gccggccgga tcggagcggg attcatcccc ccgcggatct tctccgtcga
    18181 cgcgttcacc gatttcgttt acagggaggg cctcggacgt cggaaccggc cgctcgacgt
    18241 cctcgacgcg gtcgcgatac tcctgggcat tcagcggagc gccccgggcc gtctcggcgg
    18301 ggagcgtttc ctcacggccg atcatttctt ccccctcggc gtcaagctct ttcgggacct
    18361 cgaggagctg atcgcggccg gcttcaccgg cgcctccttg aaggagaaag ggctcgacca
    18421 ggcggtccag gacgcgatct cgggcccggg ggaagacttg ttgaccgatc ggtcccgcaa
    18481 ccgtctccaa gacctctcgt attttttaga aaaattcctc gcggcggtcg aggccgccgg
    18541 cttttccacc ccggccctgc gcctccgggc ggccgccgac gggatcgacc gcgggcggtt
    18601 cgccggcgcc gatcggttca tcttcgccgg tttcttttcc ctgaacgcgg cggaggaaaa
    18661 gctgatccgg aggatgctcg gctgggagga tttcaccctc ctcgcgcatc aagcggccgg
    18721 agtcgaacgc cttctcgagc gcctcggact agagccggcg gcggccgccg gcggagggcc
    18781 ggcgggcggc tcccttccgg agatcacgtt cacccggtgt cccgatgccc acggccaagt
    18841 gttcgccctc aattccctcc tggccccgaa gctcgccgag ccggccctct tcaacgagcg
    18901 ccaggtcgtc gtcctgccgg cggccgagac gctgttcccc ctctcccatc aaacgctgga
    18961 cgacctcgcc ccggaggaat ataacatttc cctcggctac cccctgaccc ggacgccggt
    19021 cttcacgttt tttgagcggc ttctccaggt catccagtcg gccgacgccg agggccggat
    19081 ctatgcgccg gattacctgc gcttcgtcct ccatccttat acgaaaaaca tccaattccc
    19141 ggggccgggc cgccggtccg acttgacccg gatccttttt cacgccgtcg aggaggccct
    19201 gaccgaaaaa aaagggcggg ccttctgggc gctcgaggaa atcgctgtcg aaccggccgt
    19261 ccgcgaagcc gtggcccgct tgtcccgggg cgtggaaggc gcccccgtcc cggccgattt
    19321 tctcgaccac ctgcggacca ttcacgaggc gctgcttgag cccctgcgga ccatcgcctc
    19381 ggtcggcgat ttcgccggca agctgagccg cgtcctcgac ttcgtgtatg agaacggcac
    19441 ggcccgccgc cattatttct tccaccctta cgccgaggcg ttttgcgccc ggctggagag
    19501 gctggccggc tccctccttc gggacatggt cttcgaggag cgggaaggat atttcaacct
    19561 gttccgcaag atggccgcgg ccggctccgt tccctttttc ggcacgccgc ttcgcggtct
    19621 tcaggtcctt ggattttggg aaacgcgggg gatcccgttc gaggaggtct atctgcttga
    19681 tgtgaacgag gacgtcctgc cggcgtcccg gcgcggcgac tccctgcttc cgcccggcgt
    19741 ccgccgggcg ctcggcctgc ccgcgtcccg cgacaaggaa cgggagatcg agcactatct
    19801 cgacgtcctg atcggcggag cccgccgggt ccacttgttt ttcgtcgagg gccgggaccg
    19861 ggagcgaagc cgctatgtcg agaggctgct ttgggagtcg cggaagcggg atccccgaaa
    19921 aaaatcggaa acgcccgtcg ggaccgtccg gtaccgggcc gccctcggcc cgtaccggcc
    19981 ggacgagatc ccgaaaacag acgaaaccgt ccggatgctc cgggagctgt cttattcagc
    20041 cacctcgctc gacgcgtatc tccgctgccc tctccggttc cactaccgct acgtcctcgg
    20101 cctgagggag cgggaggaga tcgacgagga gatcgaggcc aaggatgtcg ggttgctggt
    20161 ccaccgcgtc ctggaggagg agttcgcgcc gcttctggga cggccgcttc gaccggagga
    20221 tttcccggcc gggcggctca gcagccgggt ggaggaactc ttccgccggg cgtttggccc
    20281 gaatctcagc ggccgggcgt acttgatgct gctgcaaacg aagcgccact tggtggatta
    20341 catcaaccgg tgccaggtcg aggtcatcct cgcctgccgg gccgaaaaca aggacattgt
    20401 gatcctggac ctggaaaacc ggatccgcaa ggcgagcacg atccgccgcg gggcgttcac
    20461 cctgtccggg cgcttcgacc gggtcgagac gcgcggcggg gagctctatg tcctggatta
    20521 caagacgtcc gccgacgaga aaaaattcgg gatcaactgg aagacgctcg atccagacga
    20581 ccgggcgacg tggccgaaag ccgtcggcag ccttcagatg cccctttaca ccgttctgtc
    20641 cgccgccctt tatgaacggg cggaagatcg catccagggc cgtttcgtca tgctcgggaa
    20701 aagccggatc ggacccggaa tcgaagtctc ccccttcgac gtcccggacg gccgccggcc
    20761 ggtcgagccg tccgtccggg cggaacgaat cgcggcgatg gggacgatca tcgatcggtt
    20821 gctggctgaa atcgtcgacc ccggcgtccc tttcaagccg gcggccgagg ccgaccgcgt
    20881 ctgtctctat tgcgacttca agggcctttg caaccgggtc tgatcgccgg ccggttgtcc
    20941 ttggatccga acgtcaatcc gggatgaaat aaacgtagat cttgcccgtc gacgggtagt
    21001 ggatcttcca gtcctcggcg atcgctgaaa gaacggagac ccggaatttc cccggctgga
    21061 cgggcagccc cgtcccgagg atctggatcg gcccccccat ctgcccggtg tattcgaacg
    21121 tcttggacgg ccggatcggc cgggttccgg tgtagccgat gtaattctgc cagttggcct
    21181 ggtagacctc cggcggcgtc tgccaggcat tgttcttcca aacctggggg taaaccgatc
    21241 cgaccgaata aagaacccgc gcggcgtcgt attcgggccg gtcctccatg tggaactcgg
    21301 catcgatgaa gatccggccg ggggcgagga tggtgatgtc ggtgtccgag atgacctccg
    21361 gggagatgtc cgtccaggaa tatgtccggg aagcctggga atcagcgccc cggcggtaaa
    21421 cagattcgtt gccggttccc cagatcttga cgtaccaaga ggtgaagcgg gtgtcgatct
    21481 cagccagaag gaccgcggtc ttgtagcccg ggccggcggc cgcctgcggc tggggacggt
    21541 tcagggcgta gttcagagtc aggtaggtgt cccgccccga tccgagcagg acgacgtagt
    21601 tgcccggatt caagaccagg ccggacagcg agccgagccg ttcctccgcc gggcgctggt
    21661 catagtattc gcggtaggcg tagatctcgc cggccgggcc gcggatcgtg aacgtgattt
    21721 cgtccacgcg gtcggggccg gagcattgca tccaggggga atccaagcgg aatcggaaac
    21781 cggaagcggt gcatcccggc gggacttgaa agccgcccgg aaagagattg caggagctgt
    21841 cccggtaggc cgtcaccggc ttgacttcgc cgggggcggc ggccggaggc gtttctcccg
    21901 agagagcgta ggccagccgg acggaggaat tccgtccgga caataaatag acgatatagg
    21961 ttccgggacc ggcggtgatc ccatcgagcg gccccgggtt ttccagggcc ggccgcttgt
    22021 cgaaatattc cgtgcattca tacaacggct ctcccgagac ggctccgcgg atgccgaagg
    22081 tgatccaatc catgcggtcg gggccttggc aggcctgcca gggcgtctgg aacgagaaca
    22141 cgaagctcga ccccgtctgg ttcgggggaa cggtgaacgt tccgagggtg atccggcagt
    22201 cgttgtcgct gaagacttcc aaggttttca gctcgggcag ctgggcggcc gcccccggcc
    22261 gggatcctcc ggcgaagaac aagacggcga gaacgcccaa gaatgcaaac acccgtccgc
    22321 ggcgatgacg tggcatgatc ttttcctcct tccggcttcc tgcgttgttt ccatttttat
    22381 tcaatgttcc cccccgatgt caagccgcga tttcctcgcg ggcggccgac ggtggattga
    22441 catttccggg ccgttgaccg aataatgaac ccgtcttttc cgggagaggg aaatccatga
    22501 cgaagcgcgc gtcgatgtgc ttgtcggcga tcggtgcggc ggcggccggg ctggccgcgg
    22561 tggtcttcta ttcccggggc atcctgcccc tgaccggcgt ctatgtgccg aaaaacgaaa
    22621 tcggctacca tctcatttac tcggccgcgg ccgtcgccgg ggcgtttctc ctggccgccg
    22681 ccctgaagcg aaaaaaaccg gtgttctgga tcggggcggg acttctcctc ctggggattc
    22741 cgctcttttt cttcgccgtg tcccgcccgc ctttcgacta cgccccgatc cggttgcgga
    22801 tcatcggccg gagcttgatc ccggcggcgt tcgcgctcct ggcggttttc gccctcaagg
    22861 gattccgggc ggcgttcggc ctgctgttcg cggccttttc ggccctgggc ctgtatcacg
    22921 cggttctgac ggggaaatgg atcgcccggg gcggcccgcc ggccgatatc gcgatcgccc
    22981 tggccttggc cgttatttcg ttctttttcg cctgggccgg tctccgggcg ctccggcgcc
    23041 cgtcgtagcg ccggctctca agcgcccaag gcggcgcgaa gcttcgccgt ttcgaatccg
    23101 gatttgcgct ccgccttcct caggatgtcg gagacggcga aagcccggga gatcgcccga
    23161 gccgcttggg cggtgacccc cccccggccg gagcctgtcg cccggcagac ggccccggcc
    23221 gcaaggagcc agcgcaacgc gtcaaaggct tcctgatgga agtactcgat cccctgccag
    23281 cgattgaggc cgagcgcccg ccggaagcgg tcgtcgccgg tgatgatgcc ggccaagcgc
    23341 cgggcgttgg ccgcggcaga tcggttcgga tcgattcctc ctccgtattc ctccaaggcc
    23401 tggaagatcg ccgggacatt cggccggcct tccccggcgc agcccgtttc acgccaggcg
    23461 tcgtcgatga tccggtccaa gccccaggct tcgatccagc cggccggact ttccttgtcc
    23521 gccgtccgcc cggccgtccg cagaaccgcg ccgcaaaaga gcgaaatcca ggccgtccgc
    23581 ggatcgagac cggccttaag ccgcgaggcg tgcggcttgg ctcccggccg cctccggccc
    23641 ggctttttcc cgggggcggc ggggccgtcc agggcggatt tcaagaaacg cagcgcctct
    23701 tggaaggtcc gggagccgga agcccggccg cgtttttcga gaaccggcag ccggaggatc
    23761 gtttcgaatt cgcgccgggc cgcggccgcg atctcctccg ggtcgatcgc tttccggggc
    23821 cgcggttcgt cctcggcgat cgtccgggcg acggcttcgg cgaacgctcg ggctttctcc
    23881 ccggcttgcc gcccgggttc ggggtcgaga ggccgtccgg gtcggaaaat cctcttcgtc
    23941 atcagccaat cgaagaaacc ggcgttggcc agctcccgga aggccgcctg gacggggcgg
    24001 agccggagat cgacggcggc ccgctctaca tcggcggttc cccggcctcc caaggccgcg
    24061 gccaggcggg catagagacg ccgttcatcg tcccggacga gacggaagcc ggtgtagacg
    24121 gcccgcgaat acgcggccgg ctccaggtgc aatccctcgc gggcgagctc gccggctccg
    24181 cggatgtatt caagtcccga gacggcgtcc ttgaagacga cccaggccga ggagtcgtcg
    24241 gggaggccga gaccctcggc cagggagacg gtcttcccgg ccgcggcggc cgacctccgg
    24301 atccagccgc gggtttcggc gtaccggttg tgatacacga caagagcccg ctctccatcc
    24361 gttccattcg agaaaacgag gacgttttcg tcgacctccc cgtcgggccg gacaaaatcg
    24421 tagagccgga agaggtcgac gccggcgaag agagcccgct tgcggaagag ggggaagatc
    24481 tcgcgccgat gccgggcgac gagatcctcg cggggcgtct cgtcgtaata cgcccggcgg
    24541 tattccatcc cgtatttttc cctgaatccc tcgatttgtc cgtggccgat catcggcagg
    24601 ccgggcaggg cggccagcag cgtgcagacg ccgaagtatt tgtcatggtc gccgaattga
    24661 tcgatcgccg tccgttcgtc cggattgttc atgaagttga cgtagcgctt gaggatttcg
    24721 gggtcgaatt ccaaggtgtc cttgatcacc cgccggtacc gggcgttctc ctcatcgcgg
    24781 agcatgttca tgaaggcgct gttgtagacc cggtgcatgc ccagcgtccg gacgaagtac
    24841 ccttccatca gccagaacgc ctcggccaga agaagcgtgc cgggcgcctc gaccgccgtc
    24901 cggtcgacga cttcgcgcca gaactccgcg ggaaaggctg cctcgaacgc cttgcggctc
    24961 agtccatgcc cggcccggga gggaatcgcg ccgcccgtcc cgggctccgg gtaccagagt
    25021 cgctggaagt gccgcttggc cagggtcatg gccgcgtcga agcggatgat ggggaagagg
    25081 cgggcgacat gcagaatcgt ttgaaggacg gcctcccgga cgcggggatt gaggaaatcg
    25141 agctgggcgg tgtcgttcca gggaaagctc gttccgtcgt tgccgtgata gatatagcgg
    25201 acgctccccg tccgtttgtc caggcgctgg aagaccacgg ccgcgtccgt ccggtcgtaa
    25261 taccggtcct cgatgcggat ggccacccgg tcgtcccagg acagatcggg tccgttgaac
    25321 gtataggaag ggaagggggg ctcgggaagg ccgatgaacc agtcgggatg ctcgatgacc
    25381 cagcgagaat cgatgcccat atggttgggg accatgtcgg aggccagccg cacgccccgc
    25441 cggccggccc gttccttgag attgaggagg gccggctcgc cgcccaggtc ggcggcgatc
    25501 gcataatcga acagggcgta ggccgaggcg gccgcctcgg gattgcccat gatctgcttg
    25561 atgcgccggg acgcggggct ccgttcccac aggccgataa gccacaaacc ggtgatgccc
    25621 gcctcggaaa gggcgtcgag ctcctcgtcc gggatctcgt ccagtctccg aatggaacgg
    25681 ccgtatttcc gggacagctg gtcgagccag acggaggcgt ttttggcgat gagaacgagg
    25741 gaggacatcc aatcgacgtc ggggctgaaa ttctccggtt ccggctcgtc cgggcgcgga
    25801 ccggccggtc cgccgaagag agcgagcgat tcctccacgg aatcggctcc aagacccggg
    25861 gcccgggagg gaccgggacc gccgccgggt ttctcctcct cccggagaag atccagcccg
    25921 ccgacgacgc ggggaagcgg acggcccaga acggcgctcc atcgggcgag gatgtactcg
    25981 agctgtccct tgagcgaatg gggcgaggcc aggacgggct cgttgagaac gtcgatgagg
    26041 ttccggcgct ccggcccgaa cggcggctgg gtttcgaaaa actcccggag gcccgagaga
    26101 atgcgcaggt aagctgtttc ccgggacagc gaatcgtcgt cgaagagctc tcgaaaggac
    26161 gcggcggccg ggttgagatt agtcaggcgg agcatcagca gctcttcgag agccgcttcc
    26221 cgccggggag gaaagcccgg cgcgtcgagg taagttcccg ccgggacgcg gttcttgtaa
    26281 acggccggcg ggggaaattc gtggaggaaa aaggccagcg ttccccggac cgtttccgga
    26341 tcgagccgcc gggcggacag gtcacgaagc gcgtcccgca aaacgtgggg attgacccgg
    26401 tcgcggtagg cctggaagac cgcgtgccgg tattcctcga ggagccccag agcgtaaaga
    26461 tcgccggctc gaacggcctt gtccggcggc cgggcggcgt tgattttatg ggcgaaaagc
    26521 cgggccgacc ggatgtcggc gaaaatcacc cgcccgtcga agataaacag cgattcgtcg
    26581 aaatgataga catcccgggc gcgacgggac acgtgaaggg cgaacatgag cgtcgattct
    26641 ccgggcggcc gcctcatcat aacaggtttt cggccgtctt gcatccggcc ggcggttggt
    26701 ttaaaaagga aaggagtttt atggagggat agccgcatgt cgcaaaaaat cagatcccgg
    26761 atcgccggcg cgctcgccgt cttcgccgcc ggggcgggcc tcgttctggc cggggccgtc
    26821 ccgcccgaac cggccaccct tgtcctgact aacggaaaga tcgtcaccct ggacgcggcc
    26881 gtccccgagg cggaagcctt ggccgcgcgg ggcgaccgga tcgtggccgt cggcccggcc
    26941 gtggagatcg cccgatacat cgggccggag accgaggtcg tggatctcgg ggggcgcctg
    27001 gccactcccg gctggatcga ttcccatctt catttcatga gcgtcggcgc atccaagctc
    27061 tcgctcgatt tgaccacggc gaaaagctgg gaggagatcg tcggccgggt cgaggcggcc
    27121 gttcagaacg ccgttcccgg agcgctcatc accggccggg gctggcacca ggaaaaatgg
    27181 aaccaggtcc cccggcccaa tatcgatgga ctgccgttcc acgacgccct gagccgtgtc
    27241 tcgcccgata atcccgttat cctgacccac gccagcggcc attcctccct ggccaacgcc
    27301 aaggcgatgg agctttccgg cgttaccgcg gacacggcgg atccccccgg cgggcggatc
    27361 atcaaggacg gagaagggcg gccgatcggg gcgtttctcg aaacggccat ggatctcgtc
    27421 cagttgggga cgtcccgggc gcgggcggcc cggacgcccg cggaagtgat gaccgaaaac
    27481 cggaagatca tcgagctggc cgaccgcgaa tgcctctcca aggggctgac cagcgtcggc
    27541 gacgccggcg tcggctggac cacggttgag ctgtacaaga ggatcatcga cgaggggcgc
    27601 ctcggcatcc ggctgaacgt catgctgagc gaaaacgccc ggaggatcga ggccaacgcg
    27661 gccgcttgga agatcgaggg ctacggggcc catcacctga gcgttcacac gatcaagcgt
    27721 ttgatcgacg gggctctcgg atcgcacggc gcctggctcc tcgagcctta cgaggatctt
    27781 ccctcctcgg ccggattgaa cacggaatcg attgaggaaa tgaaggaaac cgcccgggtg
    27841 gccgtcgaga acggcttcca ggtcgcgacc cacgccatcg gggaccgggc caaccgggag
    27901 acgctcgaca tctacgagga ggccttccga gcccatccgg gcaaaacgga cctgcgctgg
    27961 aggatcgagc atgcccagca cctccatccg gccgacgtcc cgcggttcgg gaagctgggc
    28021 gtcgtggccg cgatgcaggc cgtccattgc ccctcggacg gcccgtgggt tcccaagcgg
    28081 ctgggggcga agcgggcggg ggaaaccagc tatgtctggc ggtcgcttct gaagtccggg
    28141 gcggtgatcg ccaacgggac ggatgcgccc gtcgaggcgg cggacccgct tccgtctttt
    28201 tacgccgccg tgacccggaa aatgaacaac ggcgaagcct tcttcccggc ccagaaaatg
    28261 agccgggagg aagccctccg gtcgtacacg gtcaatggag cgtacgcggc cttcgaggag
    28321 ggaatcaagg ggacgctcgc ggccggcaag ctggccgacg tgacagtctt cacccgggac
    28381 cttctgactt gtcccgagga ggagatcctt tccgccgagg tcgcgatgac gatcgtcggc
    28441 ggaaaggttc tctataagaa gtaaggggag agcctacggg ccttaaaact tcatccccaa
    28501 gccgattccg agcgtcaggc cgccgacatt gatgtcgaaa tcggccggct tcatgctgca
    28561 ggtgctgtat ttcagccggg cgtccagaat gaagctcgag ccgaggccga cggccacgcc
    28621 gccgaggccg cagaagccga gcttgctctg tttggcctcg cccatcggct cgttgctttc
    28681 cttgaacatg tacatcccca gcccggcgcc gaagtagggg gagatcatcc cggcttcgaa
    28741 ccggtagagg gcgccgccct cgaaggccag gacgctcatc ttggtctctt cctcggtgta
    28801 ggtggttttc cccgtggccg agcggtagtt gccttccacc cagccggcca ggctttgacc
    28861 gccgacgcgg acttctccgc cgaagaccag cccgttctca tacacgtcct tgtaggccgc
    28921 gtcggaggcc aggaaatagc cggcccgcag cagaaccatg gagaacgtgt tcttgcctcc
    28981 cgattcgccg accacctgat ccgggagcgc ggccggggcg ggtttcgggg cggccggccg
    29041 ctcaaccggc tgcatctcct ctggaagcgc cggcgcggtt tcggccactt cctcgacgac
    29101 ggcagcgctg atgaagcccg gcgtcccgtc ggccagctga atgaggtacc agtcgccggc
    29161 tttttcgatg accgtgaaga tctcgccctt attggccccc ccgatgacgg gagccgaggc
    29221 gaagcccgac tgccggatgt tggcccgctc gaccgtgacc cggatcttca gatcggcgcc
    29281 ggcggtcaag ccggagggca gaaacatggc gatcgccgcg gccgcgagaa tcatgaggac
    29341 gagttttttc ttgaacatgg ctgctccttt aatccagctc ggccgtcacc aaggccggcg
    29401 ctccctcgcg gcccgcgctc gtgatcgcgg cgatggcgta ctgggtggcg gcctcgccgg
    29461 cgttgcggcg ggaatacgtt gtctgatcgg cgttgacggt cgtcaacggc gtataggtcg
    29521 atccggaaac cgtatagatt ttgaacgagg cgatggtcag gccggtgttg tcggcgctgt
    29581 tctgccacgt cagaatgtcg atgtactccg cctgggagaa cgaccggttc aagaccttct
    29641 tggctgaggc ggtcggagcg tagatgtatt ggtaccaggc cttgatggtc ttgttccggt
    29701 tcatcaccac ggtcaccggg ttggtcgttc cggtcgcgtc cccggtccag ccgttgaact
    29761 gaacatagtt ttggggaacc gccgtgacgg agacgctggc gttgggggga taagcgtaag
    29821 cccctggggc gggcgtcgtc gtgccccagg tgttgtttct ctggatggtc agaatgaagc
    29881 tgcccagcga ttggagggcc ttgtcgattt ggatccgggg gatcccggcg ttgtatccgt
    29941 cgcagacgct gtagatcagc ttgcccttcg ttcgaagggc ggtcagcagg gtcgtcacgc
    30001 ccgcggtcgg gaacgcctgc cggagcaggg cccaggcgcc cgcgacatgg ggcgtcgcca
    30061 tggatgtgcc gttccaggag gcatagcttg aatccgagtc cccggtggag gaatagatgg
    30121 acgaacccgg ggcgtacagc cgctgggtca ccgaatccca gttgttgaag gaggaatccc
    30181 cgtccgactt ggtcgaggat ccgaccgaga ccgaggtcga aatgcaggcc ggccagctga
    30241 tccgtccgca ggaatagctg ttgccggtgg cgatggccgt ggcgatcccg gccgcccgca
    30301 gcaggtcgat ggccgatttc acggacgagt tgacgctgtc gcagaaattc ggatagaggc
    30361 ccccgcccag gctcatgttg acggaggcga ttttataggc gcttcggagg gaatagacgt
    30421 aattcaatcc ggccaggatg tccgaatccc aagcggaaac gcagggcgag ccgccgcaat
    30481 cgccggccgt gaacttggag aaaatcttca cggcgatgac gccggccccc ttggcgatcc
    30541 cggccagggt gccgtaattg ccggtcgcga tacccgcgac gtgggtcccg tggtcgaatc
    30601 cgtcatacgt gctgggataa tgaacggccg accccggtcc gttcatgacg gccaggccgt
    30661 tgggacaatc gccggcgccc ggcaggccgt cccggcccat cgcccggcag gcctcgatga
    30721 tggttttgcc ggtgaagaac tggtgcgtct tgcggattcc ggtatcgagg acggccacgt
    30781 accaccccgc gcccgtgtag cccatcgccc agacggccgt cgccccgacg agggcggccg
    30841 tgtcgtccag gaggggatcg acccggatct cgtcggccga aagcccgcct ttcgggccct
    30901 ctccgccggg tccgggcgga tcgatcagct tgcgcggaag atcctcggaa atatccagga
    30961 cattcgggga attttccagg acggccaggg cgttgggcga gacccgcagg gtgatgtagg
    31021 ggatgtaatc gaagccggtg acgaattcga aatccgtgcc ctggagctcg gtgaggaccc
    31081 gccaggacgc gtactcgatc gttttcgcca agacctgatc ggccagcgac cgctccaggg
    31141 cggtttccgg cgtttcatcc acgccggtga aacgcgtcga atcggcggtc aagttttcga
    31201 tgtcggggac ggtgaagtgg acgatcactt ggacccgccc ctcggcccag gctttctccc
    31261 gaagctggcc gattccggct tggttttttt cgtgctgggc gagccccgcc agcggccagg
    31321 cggccagcac aagagccgcg agcaggtgca cggcggcccg tcgtgttttt ttcctcatgg
    31381 tttttcctcc tccctaaggg ccggcgatcc cgcttgaccc gcgccggcga agacatcctt
    31441 cacctccgcg ggccgaacgt ttttccggct aatgtctgct gaaaatatat tcttggattt
    31501 ttcccaagtc aagaaaaatc tcgagctccg agaaaaatcg ttcgaaggaa gaaaataatc
    31561 attgattaat gaaacagatg gatattacaa tcaagttgtg aaaaccagcg agacaacgaa
    31621 cgaggatacc gtccggcgct ttcggagcct gctccgcgcc ctggaacgcg agacgatccg
    31681 tcccttcgac agccgcaacc ggtgctgcgg cctgaccttg tcccaatgcc atgcccttct
    31741 ggaaatcggc cggcgggggg agatttcctt ggtcgatctg gccggggtgt tcggcttgga
    31801 cgccagcacg ttgagccgga ccattcaagg attggtccag gccggcctcg tcgaccggcg
    31861 gccgggcgag cgcgatcgcc gctatatcgc catttccctg tccggcaaag ggcaagcggc
    31921 cttccgggac atcgagcgcc gcttcgacga gcgcgtcgcc gccgttttgg agcggatccc
    31981 cgctccccgg cgggaggcgg ttgtcgaggc ggccgccctg atcgccggcg ccgttcgggc
    32041 cgaaaacgaa tcgggcggat gttgtccggg aagaaaagga aatgcctctc gttaaatttc
    32101 cttccgccgc cggcctcgaa aaggagagct gttgctgcgg tcccgattgc tgtcgcggga
    32161 cgaagggcgg ggagaaggcc ggtgtcttcg accggtccat cgaccccgtc cggttcgcgt
    32221 cagggaggcg gatgaccgcg gtcggggaga tccccaccgt cgccgcgcgt ctcggcctcc
    32281 gcgaccgatg gggggccctg cgcgtgcggc tggggatcgg ccggggccgg tatgcgatcg
    32341 cccccgggct ctatgcgctc ggatcccccg atccctggtc tcctgtcttt gtcacagcca
    32401 actataaatt gagctttgac gcattgcgga cgaagcttcc cgggatcgat gcctggatcc
    32461 ttgtgctgga caccgggggc atcaatgtct ggtgcgccgc ggggaagggc acgttcggcg
    32521 cggatgaagt tgccggccgc cttgaagcgt ccgggctcaa aaagatcgtt ttccaccgga
    32581 cgctgattct tccccagctc ggcgcgccgg gcgcctccgc tcccgagatc gcccggcgga
    32641 ccgggttccg ggcggtgttc ggcccggtcc gggcggctga cctgaaagcc tttctggacg
    32701 ccgggatgac cgcggctccg gagatgcgcc gggtcaggtt ttcgctccgg gatagactca
    32761 tcctcgttcc catggagctc gccggcgtcg tccgcagcag attttttctc gccgggctgg
    32821 ggctttggct ggcggcgatg atcgccggaa tccgcggcgc ccccctggct ggagccgccg
    32881 cggccggggc gattttcgcg ggggcggcgg cgactcccgt cctcctgcct tggattcccg
    32941 ggcggatgtt cgcggcgaaa ggcgcgatct tgggggttct ttgggcgtcg gcggtgtgct
    33001 tgatcggagg attcccgtcg ggcggcccgg cggatttggc cgcggccgcg gccgtgctcc
    33061 tgtttttgcc ggcgctttcg gcgttcctgg cgatgaattt caccggttcg tcgacattta
    33121 cgtcgctctc gggagtcgtc gccgagatga aggttgctgt tccggtcatg ctcacggccg
    33181 ccgggctcgg cttggcggcg gccgttttgg cggccgtcat cggaggatga aggggagaac
    33241 gatgcccttg cgtcatttaa aaaatgtcac aaccctaaaa tatgatcccc gcaagtgcgc
    33301 cggctgcggc ttgtgcgcgg ccgtttgtcc ccacggcgtc ttcgagatgt acggccggac
    33361 ggccgcggtg acgggccgcg atcggtgcat ggagtgcggc gcctgtgccc gcaattgcgc
    33421 tttcggggcg atctccgttc gggccggggt gggctgcgcc tccgccgtcc tttggagcat
    33481 gagacggagt tgatcgcccg cggttcgcag gctcgatgac gaatcgcgtg gatcttcttg
    33541 atctgtttga agacgaaacg tcataacccc ctcattctca ttgacaagag agggtcctga
    33601 gcctattttg aaaatggagt ccgcggagcg gacgacgggc tgtttcgacc ggggcggacc
    33661 gagggcctcg gcgcatggga gtcggagcgg cggccggcgt gagcggttgc ggcgatgagg
    33721 cggccgccgc caaggacgta tctgtttgga ggcggccttc ggtccgcccg ctttgagcgg
    33781 cgggcggttt ctcggattgt ttggggtttt ccctttatgg tatgacgatc tcttgatcgt
    33841 cggcatgccg ttttcggcat tccgcaatga aagggagggc cccttttctt tttagccatg
    33901 tccctccttt cctcgtccgc ggaccggtcc tcccgcccat gcgggaggtc gacaaggcgg
    33961 caggaatcta cgacccaaca ggatttccaa cagacgacga cgttctcggc gacacgtcac
    34021 tcggcctcgt cccggtgcct gacgacacct ctccggtcac gttcaagtcc ttccccatca
    34081 tgggaagatc gatgtcccgg ccggagccgc gatgtcaggc aaggaggaat ttccgtcggc
    34141 ccgtatcgac gggaccggcg gccggccgga ttccggccgg cggaagaatg tcctataatg
    34201 ggtccttgaa ttttccccgg gcgagcctcc ccaaggtccg tccggctgga ggtgaatcgt
    34261 gtctttcatg aaaaacaaga tcgtctttgt gaccggggcc agctccggcg tcggccgggc
    34321 ctgcgcccgg gcgttcgccc gcgagggcgc gtcgcttctc ttggccgccc gccgggccgc
    34381 ccgcctgaag gagctggcgg aatccctggc ggccgaattc ggagccgccg tcctcccctt
    34441 cgccctcgac gtccgcgacc aagccgccgt ggcccgggcc gtggggggtc ttccggaaaa
    34501 ttggaaggcc gtcgatgtcc tggtcaacaa cgccggcctg agccgcggcc tggacaagtt
    34561 ccaagacggt cttctttcgg attgggagga gatgatcgac accaacgtca agggcttgct
    34621 ctatgtcagc cgggcggtcc tgcccggcat ggtgacccgc gggcgcggcc atgtcatcaa
    34681 catcggctcg atcgccggcc acgagatcta tcccgccggt aacgtttatt gcgcctccaa
    34741 gttcgcggtc aaggccctga gccggggtct gcgcctggat ctttgcggaa cgaacctccg
    34801 ggtcagcacc gtcgaccctg gaatggtcga aaccgagttc agtgaagtcc ggtttcacgg
    34861 ggacaagacc cgggcggcga acgtctaccg gggattcacg cccctcactg ccgaggatgt
    34921 ggccgaggcc gttcttttct gcgccacccg gccggcccat gtcaatatca gcgaagtcat
    34981 cgtgatgccc accgcccaag catcgaccat gttggttcat cgacgggagt cgtgaaccaa
    35041 catggtcgcc ctccatcgcg acgcgatcag ggcgacgacc ccggttcatc gacgggagtc
    35101 gtgaaccaac atggtcgccc tccatcgcga cgcgatcnnn nnnnnnnnnn nnnnnnnnnn
    35161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35221 nnnnnnnnnn nnnnnnnagg gcgacgaccc cggttcatcg acgggagtcg tgaaccaaca
    35281 tggtcgccct cccttttcta tttcatgatc ttgatcagcc ggcctttttc gaccttgtcc
    35341 tcgagagcca gggcgttaag gaaggcgatt gtcggccact tgtcggacgg cacgccttgt
    35401 ccggccagga attccttcag ggtttgagcc gtccccgccc ggcggacggc gagctttttc
    35461 ggctggacgg ccaaccgctt gggatcggag aggttggcga agctccggac cgtccggtcg
    35521 aacgtgttcc ggaaagaagg ataatcagcc ttggccgcca tgctcgtgaa cgtgtagatc
    35581 atgccgttct tggcgatgca gtcgaaatcg acggccgtga cgggaggcgc ctgtccctcg
    35641 ccggcttcct cgctgaccag ataggttccg cgggcggcgc gcagaccgtt gatcatccgg
    35701 ttcgttcggg agacttcctg gattcggacg ttttcgaggg cttcaatccg ccggccgagg
    35761 tagaggtccg gcgagaggtc cgtttcctcg gccgtgagga tcatgcccgc cttttcgtcc
    35821 ttgctggcga cgacgacctg gcggggcgtg ttttggacgt tccagccggc cgggatggag
    35881 aaaacgaatc tcatttcggg atggtagaag gcgtcagcct cgacgtatcc ctggcgcgga
    35941 tcttcgccgt agatcagccc gtccatcttg gccagcagtt cgttgcggag aatgttccgt
    36001 tccgcgtcct gcggctcgag catcttttcg gcttcctcga tccgcttttc cgtcaagggg
    36061 tgggtcgaga ggaaattcgg cagcttcatc ccgccgccgg ccttctcttc cagcttctgg
    36121 atggaccgga agaaggggat catctgggcc ggggaatatc cagcgccgcg ggaatagagg
    36181 acgcccaggc tgtcggcctg gtattcgtcg tcgcggctga atttcaggaa gagcacctgc
    36241 aggccgacca tcagaaaggg cgcgaccttg gccacgtccc ggctgagaag gctgccgacg
    36301 actaatccgc cggtcagcag gagctgtttg ctgtattccc ggacggaatg ccgggcgttg
    36361 acgtgaccga gctcatggcc gacgacggtc gccagctcgg cctcggaatt catcatggcc
    36421 aaaaggccgc gggttacgta aatataaccg cccggagcgg cgaaggcgtt ctcgaccggc
    36481 gtgtcgagga cggcgaaatg gtactccagc gcgcggcgat gggtgtaggg aaccatccgc
    36541 cgggcgactg tgttcaaata ggcggagagc gcggcgtctt gatagagccc gtactccttc
    36601 cggatgctct cgtcggtggc tttgcccatc tcgatttcct tggcttccga gacgagcatg
    36661 agctcctgct tcccggtgac gggattcacg gcgcaggaga gggccccgga cacggcggcg
    36721 aggacgatga cggcggcgat gcggcgggga gggaatctca ttccagcctc ctattccgat
    36781 ctctgagcga aatctactcg aaacggaagg cgaattcaac ccgtccttcc gcctgggacg
    36841 cgctgtcgcg gaaggggctc gccccgggac gttctttaat tttcgcctcc aaggtgatat
    36901 attaccggcc agatgtgctg cgaagccgct gtcttgcctc ctcggccggg atccctcccg
    36961 gcggacgttc ttgacggcgg atctgtttct ttcaggagaa tcattcatgt ttaaagggca
    37021 tcccaagggc ttgttcgtgg cctttttcgc caacatgggc gagcggttcg gcttttacac
    37081 gatggtcgcg attttcgtcc tgttcatgca ggccaaatac gggatgaccg cggccacggc
    37141 cggcttcgtc tacagcatgt tcatggtctg cgtctatttc ttcccgctgc tcggcgggct
    37201 gctcgcggac aaggtcctcg gctacggccg gaccgtcagc ttgggcttga tcgtcatgtt
    37261 tctcggatac gccctgctgg cgcttcccac gcggatgaac acggggcttt ccctcatcat
    37321 cgcggccatg gccgtcatcg ccgtgggaac cgggcttttc aagggcaatc tccaggccct
    37381 ggtcggaagc ctctacgacg atccgaaata cgacgccctg cgcgaccggg ctttcacgat
    37441 cttctacatg gggatcaata tcggggcgat gttcgccccg agcgcctcgg aaatcataag
    37501 caattgggtc cttaaggggg ccggattgac atatgacgcc cgcatccccg ctctggccaa
    37561 cggctttttg aaaggaacgc tgaccgaggc cggcgcctat cttgatatcg cccggaccca
    37621 ggacgccggc gccaccctgg aaacgctggg gacgttctcc gagcgctaca tcaacgcctt
    37681 gagcaaatcc taccatttcg gattcggggt ggcttgcttg agcctgatcg tgtccatgct
    37741 cgtcttctgg gcgttccgga aatattacaa gcacgccgat ttcaccgaaa aacaaaaggc
    37801 caagtccgag gccctcaagt cccaggtcgt cgagctcacg cccgcccaga ccaaggaacg
    37861 gctcgtggcc ctggggcttg ttttcttcgt cgtcatcttc ttttggatgg cgttccagca
    37921 gagcggcctg acgatgacgt tcttcgcccg ggattacacc cagctgaacg tcggggccct
    37981 gtcctacatc tggttcgaca tgatcggcct gctggggatt ttcctggccc tcggaggcct
    38041 gatctttctg ttccggaaag gctcgggaaa gacagcccgt ctggccggcg gggcggcctt
    38101 cctcgtgttc ggcgccctgg ccgcctggcg gtacgccggt tattccgccg tcaacgagtt
    38161 ccagccccag cgcttccagc atttcaaccc gtttttcatc gttttcatga cgccggtcgt
    38221 cgttggattc ttcagttggc tgaacaagcg gaacaaagaa ccctcggccc cccgtaaaat
    38281 cggctttggg atgctgatca cggccgtctc cttcgccgtt ctcgtcatcg gatcgctcgg
    38341 tctgcccagc cccaaacggc tcggcggcct cgtcgccccg gccgaatccc aagtttccgt
    38401 ctactggctc atctcgacgt atttcatcat cacgatcgcc gagctgttcc tcagcccgat
    38461 cggcatttcc tatgtctcca aggtcgctcc gcccaagtat aagggcttgg cccagggcgc
    38521 ctggttcgcg gccacggccg ccggattgta tctcatgggc ttggtcggcg gtttgtggat
    38581 gcgggttccc ctctgggcgc tctggctgat tttggtcgcc gcctgcctcc tttcggcgac
    38641 gttcatgttc tccgtcatga aaaaactcga acgggcctcg gggcaataag cgcgatcaga
    38701 gggagacacg atacccattt cccccttttt caacgtcgtc cttatcgcgc cggagattcc
    38761 gcagaacacg ggcaatatcg cccggacctg cgcggccgcc ggagcgcgcc tccacctggt
    38821 ccggccgctg ggattcatcc tcagcgaccg ccggctccgg cgggccggcg cggattactg
    38881 gagcggcctc gaccttgccg ttcacgagag cctggaggcg ttcttccggg agacgccccc
    38941 cgaacggtgc ctctttttct cgcagaaggc cccacggcta ttttcggaaa tgccggtcga
    39001 ggaaagcgtc tttctggtct tcggccggga atcgaccggc ctcccggagg agatcctggc
    39061 cgcttgccgg gagcgaagct tccgcgtccc catgcgcccg ggcgcccggt ccctgaacgt
    39121 gtccaacgcc gcggcggtcg tcctgtatga agctctccgg cggcgcggat actccggctt
    39181 gatatgacat cgttctcaag aatgaggtga ctcatgaaca tcgtccgcgt gccccggctg
    39241 gtcaagccgg gggatttgaa tcatcacggc tcgctctatg ccggccgggc ggcggaatgg
    39301 ttcgttgaag cctgttatct ctgcgccgcc caggccccgg atttgccggg aacgatggtc
    39361 tgcctggcga tccgagacct cgttctctcc aagccggtcc ggggcggcga gaggattcaa
    39421 atcgaaaccc ggccggtcaa ggccggccgg accagcctga cgatttacgg aaagatcacg
    39481 acgcggtccg gaccggcccg ggccgaggga ttcgtcacgt ttgtctgcgt cggcaaaagc
    39541 ggccggcccg tcgcccatcg gctgcgcctc gatcccccga gggatgccga ggaaaaggag
    39601 attcagcgga aggcgcggaa tttctgaccg gcgtctcccg cccttccgcc ggcctcgcct
    39661 tgccggggaa aggcgtccgt tttatgatct tcttgatgga cggccccgcg gacgggacgg
    39721 aaacggtttc gatcctgaaa gggaaaacgg cggagccggg agggaggagg ccatgaaacg
    39781 gaacgatttt ctcaaggccg tctgcgggat cggcttggcc gggcgggcgg ccgccctgaa
    39841 cgccgggccg gccggccggc tgaaaccgga agaacaagaa aaggaaaggg atcgcttcaa
    39901 ggaagcttgg ctccggacgc tgatggagaa catggaaaaa accttggatc ccgccgtccg
    39961 ggaacggttg atgaacgatt gcggccggga gtgcgcccgc cgcggcgtcc tgatggaaac
    40021 cgccgggaaa tttcaaggcg acgtcgccgg cttcgtccgg aatctcggcc cgaaagtcgg
    40081 cggggacctt tgccgggtcg agggaaacat cgtccactgg ggctatcccc ggtgctattg
    40141 cgagctggtc gcggccggtc cggagcggct tccggacgcc tactgcctgt gctcggccgg
    40201 ctgggtcctg gaagtcttcg aagccgtcgc cggccggccg gtccgagtgg aggtcgtcca
    40261 gacgattaag cgcggcgcgc cggattgccg gttcctcgtg cgcctctgaa gccgggcggc
    40321 cgcgtttccg tcccggtttt cggatttcca cgacgggagc cggagctttg gatgcgattg
    40381 gcccggaatc ctcgtttttg gtacactggc ccctcatggt cgactggctg cgtggattat
    40441 cgccgtttct ccaggccttg ctggccaccc tcctgacctg gggagccacc gccctgggcg
    40501 cttccctcgt cttttttttt aaaagaatca aacggagcgt cttggacggc ctgcttggat
    40561 tcgccgccgg ggttatgatc gccgcgagct tctggtcgct tctggccccc tcgatcgaga
    40621 tggcccggag acaggggcgc atcccctggc ttccggcggc cgccggattt ctcctcggcg
    40681 ggctttttct acggttgatc gattattttt tgccgcatct gcatgccggc tttgaaaagg
    40741 tcgagggtgt caagacgaac tggaaccgca cggcccttct cgtcttggcc atcacccttc
    40801 acaatatccc cgagggcttg gccgtcgggg tggctttcgg cgcgatcgga ttgggcggcg
    40861 gcgcggccac gttcggagcg gccgtcgcct tgaccatcgg aatgtgcctg cagaacttcc
    40921 cggaaggcat ggccgtctcc gttccgctcc gccgggacgg cctgtcgcgg tcgaaaagct
    40981 ttttctgggg ccagttttcc ggcttgatgg aaccgctggc cggcgtcgcc ggcgtcctgg
    41041 ccgtgtcccg ggccgaagcc cttttgcctt gggcgctggc tttcgccgcc ggggcgatga
    41101 tctatgtcgt tgtcgaggaa gtcatccctg aatcccaagt cggcgaacac accgacatcg
    41161 cgaccttggg aacgatggcc gggtttgtct tgatgatgat cctggatatc ggattgggat
    41221 aaccgcataa ccgggaccat gtcccggtcg aaaaacaagg agtccgatca tgatgcgttc
    41281 cgttgtcgcc gccctccccc cggcggaaat cgatgggttt ctggcagcca tcgagctttt
    41341 ttccgacttg accccggccg agcgggaagc cgtcgcggcc cggttgaagc ttgtctccta
    41401 tgaagccggc caggccctct tcgaggaaaa ccagccccgg gaaagcctct tcctgattta
    41461 ccggggagcg gttcgtcttt acaagaaatc cccgctgggc gaggaaaagg agctggcccg
    41521 gttccgccgg ttcgatttcc tcggtgaggg agccttgatg gacgactacc cgcacgcgac
    41581 caccgcccgg gcggaggagc cgaccctcgc cctcgtcttc tcccggacgg acgtcaccgg
    41641 gctcctggcc gcggagacgt cgctggcggc caagatcctg tcccggacgg cccgggtcat
    41701 ttcccggagg atgcgccagg cgaccaacca agtcgtgaac gcggccgccc agtacatctc
    41761 cggccgcacc cgccgggagc acgatctcct cggcgaacgc gacgttccgg ccgaattcta
    41821 ttacggcgtc cagacgctcc gggcgacgga gaattttccg atcactggaa tcgccatcag
    41881 ccgttttcca gagctgatcg tcgccttggc ccaagtcaag atggccgccg cccgggccaa
    41941 ccgggacctg ggactgctgg atgccggcgt cgcagcggcc attgacggcg cctgccggga
    42001 gatcatcgac ggccggatgc acacccattt cgtcgtggac atgatccagg gcggggccgg
    42061 aacctcaacc aacatgaacg ccaacgaggt catcgctaat agggccctgg agcttctcgg
    42121 ccgggagagg ggggattacg cccattgcca tcccaacaac cacatcaacc tctcccagtc
    42181 gaccaacgac gtctatccca ccgccctgcg tctggccttc atggccagca cctgcacact
    42241 ggtcgccgtg ttgaaaaagc tcatcgcctc cttccgggcc aagggcttgg aattcaagga
    42301 tgtcatcaag atgggccgga cccagctcca ggacgccgtc ccgatgactc tcggccagga
    42361 gttcgaggct tttgccgcca ccctggggga ggaggtcgag cggttggagc agaacgccgc
    42421 tcttttcaag gaaatccacc tgggcgggac ggcgatcggc acggggatct gcgctccggc
    42481 cgattacgcc ggccgggccg tcgcccacct aagcgagatc tccggcgttc cgctccgcct
    42541 cgctccggac ctagtcgagg ccacccagga tacgggagcc tttatcatgt tctcctcggc
    42601 cgtcaagcgc ttggccgtca agctctccaa gatctccagc gacctgcggc ttctcagctc
    42661 cggcccccgg gcagggatcc acgagatcaa cctccccaag aggcaacccg ggtcgtcgat
    42721 catgccgggc aaggtgaacc cggtgattcc cgaggtcgtc aaccagat
//
LOCUS       DAOOXH010000011        41457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_791_length_41457_cov_8.719724, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000011 DAOOXH010000000
VERSION     DAOOXH010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 41457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41457
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_791_length_41457_cov_8.719724"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PKV64_02655"
     CDS             complement(<1..435)
                     /locus_tag="PKV64_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPN16015.1"
                     /translation="MSFDKIAARLDLFRDEMIDLQIKLASIPAISPASGGEGEARKAD
                     FLIAYLRESGITDIQVIRAPDLDAPAGYRPNIVARYPGRNSARTVWIMTHLDVVPPGE
                     ISLWQGDPFKPWVSSGRIYGRGVEDNQQGLVASIFALKAFHDL"
     gene            793..1929
                     /locus_tag="PKV64_02660"
     CDS             793..1929
                     /locus_tag="PKV64_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPN16016.1"
                     /translation="MRIGFDVRPFLRQETGVGVYFKNLLFELAALDRDNEYCLFSASY
                     KDRFPPAKIPPFEKLTFVDKRWPVRAVNAAWDMFGRPRLDRIFGGRLDITHSPTPLRL
                     PASGKAIVTVCDLFFLEEPEKADREARTRFLRKTGRSLRSADGVITISEYSARAIRER
                     FGLDEEKIMVTPLGLNRFFEDPAAPEETAAVRRRFDLPGEFLLFVGALEPRKNVPALI
                     EALALVHERHGRIPLVLAGRSGGDGENVDRAIERRGLGAWVRRTGYLPDVDLRALYRT
                     AAALVFPSSAEGFGLPLLEAMACGLPAAASGVSALPEIGGDAALYFNPEDPEEIARTV
                     IRLLADSPARESLKIRGLRRAELFPWRRTAEATLGFYRRTAGKT"
     gene            1926..3167
                     /locus_tag="PKV64_02665"
     CDS             1926..3167
                     /locus_tag="PKV64_02665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPN16017.1"
                     /translation="MISVLFVSHSAELNGAELWLLETLRRLDRRMFVPALIIPRPGPL
                     GPAAEKAGVSVDVVPMKWWVTEKAKVWRQPAARLLNRRAVARIGRIARERKAGIIFSN
                     SAATFGGALAARRLGIPHVWAIHEILAGDRAFLHSFRGSRALTRFILDRSARVIVNSE
                     STRRAFPPSGKIALVYNGVEIRSAAGERRGIVRAEFGLEEGSRTIGVVGKLYPGKGQK
                     EVLLAFARLAGRYPDLKLIFIGTAADRRYEQDLRRLVRLNDLARRVVFAGFRPALSEV
                     LQNLSVVVVASVVDSFGRAALEGMAAGVPVLAVKAGGLTEIIRPGRNGFLAESRDPEV
                     LAAALAEILDHPERWPAVVENGFRTVREDFSIEKQVQGVTRILLEVCGRTILCPSPPE
                     AGSNGPLFASFPGGGKGDGRG"
     gene            3164..4051
                     /locus_tag="PKV64_02670"
     CDS             3164..4051
                     /locus_tag="PKV64_02670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPN16018.1"
                     /translation="MSTPLVSVILVYFNDRLHLASCLESVEAAGRDVALEAILVDNDS
                     TDGGLEDAAAFPSVRIIRNGDNPGFGAANNRGVRESRGEFLLFLNTDAVLQPGAAAAL
                     LEAVRADERTAAAGPLLFSGGGKVQVSFGGRVDFVGQLVQKFIKNPLTARALRRRPRG
                     AEAGWLSAACLFCRRGAFEAAGGFDESFFLYFEDIDLCFRFRKAGGRLRFVPEARVFH
                     AGGATTGRRPRESRLEYRKSQMLFYRKHASPASLRLLRLSLRIHLGLQRILGRFRGED
                     GRAMRAAYRKLLKPERGIP"
     gene            4048..5190
                     /locus_tag="PKV64_02675"
     CDS             4048..5190
                     /locus_tag="PKV64_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPN16019.1"
                     /translation="MRRLKVLEMIDKPSLGGGQAVLLALARNLNRNRFDVSVCAGGGG
                     PLEEEVRRAGMTFHPVEFRKNPAAGPGRTLIRLLASQGFDLLHTHGGVAGLYGRRAAR
                     RAGTPVVVHTIHGIHYLHYRNPLLRRAAVLLERILSRSTSAVVCVSEADLAQARRHRL
                     APDGRLRLVRNGVPSIEAASDPDRVSRGRALCSELKLRAPVIGTVARLHRQKGVEYFV
                     EAAPDILRRHPEAFFLVVGGGPLESRVRKGIERLGLTGRFILSGERPDAFDLLSAFDI
                     FVLPSLWEGLPLVLIEAAGFGKPIIATDIDGSREIIRDGETGLLVPPADPAALAEAVV
                     RLLEDEALARRLAERARNEIPAAFSLERMVAETERLYLDLAAANGL"
     gene            5202..6320
                     /locus_tag="PKV64_02680"
     CDS             5202..6320
                     /locus_tag="PKV64_02680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN16020.1"
                     /translation="MKNARVALIHDWLTGRRGGEKVLEVLAEIFPAAPIYTLFHFPGS
                     QIPEIEKRVIRTSFLQGMPALRRHYRWYLPLFPLAAESFDLQGFDLVVSSSHCAAKGI
                     IPKPEALHVSYIHSPVRYAWNQYAAYFGPGKLGFPAKYIVPPMIHGLRNWDVTSAARV
                     DRFIANSRAVASRIEKYYRRPAEVVHPPVDTEFFVPPDSGPERKYFLAVTALVPYKRI
                     DLAVAACNLGKFPLKVVGDGPEMRSLRAAAGPTVEFLGSVSGDDLRRLYQEAIAYLLP
                     GEEDFGISAVESQACGTPVIALARGGALETVRPGETGLLFEEPTPAALAAVLDKCRGL
                     NFNKASLCAHARTFSRDGFKAKMETRLAALWREFKEPR"
     gene            6293..7771
                     /locus_tag="PKV64_02685"
     CDS             6293..7771
                     /locus_tag="PKV64_02685"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HPN16021.1"
                     /translation="MAGIQGTKVISRYQARLSALFVASDVLAITATFIYSYAFRFHSY
                     FIPVDPAKGIPTWRSYLFVLPLFLAVHLAIFFLQGFYKSRLRRTKIDDFFSITINAVL
                     TLIVVMAVSGYLNAYSGGTAPLFRVNIVKLSHLFLAVYFIGVIFTISFFRNQIYFFMK
                     RRFARGLNLRSVLVVGAGEMGVAVAQKLFQYKDLGFVVKGFLDNHKPAGKIIQVDGGI
                     PVLGPVSDLGKVIEAQGIQEVYVALELENYPEMLEALQVVNKYPVNLRLIPDLFQLLT
                     LKANVQDLDGFPVISVDEVPLRGARRVLKRLFDIVVSGLALIVLSPLFLIVAILIKMT
                     SRGPVFYSQERFGLDGKRFKIHKFRTMVQNAEKATGPVMCAPDDARITKVGRLLRRYS
                     IDELPQLVNILRGDMSLVGPRPERPEFVKEFTDSIPKYMLRHKMKSGLTGWAQVHGLR
                     QGTSIEKRLEHDFYYMRNWSFTLDLKIIWMTFRRGFIDKSMT"
     gene            complement(7778..8389)
                     /locus_tag="PKV64_02690"
     CDS             complement(7778..8389)
                     /locus_tag="PKV64_02690"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPN16022.1"
                     /translation="MSRTDRPEAEDWVSSALTVPNLLSAFRILLVPLFIWTFLERQTI
                     EPLVIFFIAAISDLFDGFIARHLHQRSRLGTILDPAGDKLLTAASYILLTLPGIGGPN
                     RVPLWLTIIVFARDLMIVGGVLWAYLIMGVKTVLPVFLGKLTTAFQVGTVFLVLLTNH
                     LGKQAGWMLVFYALTAGFTLASWILYYQRGLRLLRERRAAEES"
     gene            complement(8386..9723)
                     /locus_tag="PKV64_02695"
     CDS             complement(8386..9723)
                     /locus_tag="PKV64_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPN16023.1"
                     /translation="MVKKTAGTLAAVLAAAACATISPPAEPTIYLENPPAEISAALSL
                     DDRIAVDKVWNLLRLERADKAEKAVLRLGESHPFYWTAMGYIALLRNDLTAAEADFVG
                     SLTVSPDAVTSQLGLGQVYRRWGRREDALRSYLEVLKRDPENAFARREADELRTAIVE
                     SLILEADAAARTGKTAAAKEAFLKVLEYAPKLQIAHVSLARLFTREKDYPRALFHLGL
                     AAENNPLDRTVLREYAETLYQMNQFSRSLDAFQKLQALDPSDKTVAERLEALKTRLGV
                     VDLPDEYREIPGLEAVTKEDVAALIGAKFNDIWIGVAVRPPVLVDISTSWARTFIVKV
                     AASGIMEIYSNHTFQPKKTMNRAEMAETVVHLVEYLRKKGRTIVVQIPPERINLADVP
                     ADHPYAPAIIAAVSFQLMDIFSDRTFRPDQPVPGAEAIRILDLLAGLAKEPGK"
     gene            complement(9717..10976)
                     /gene="hflX"
                     /locus_tag="PKV64_02700"
     CDS             complement(9717..10976)
                     /gene="hflX"
                     /locus_tag="PKV64_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPN16024.1"
                     /translation="MNRTVLVHLMTSSRDRSEAIESMAELEDLARAAGARIARKVFQT
                     RASPDPRTFIGGGKVEEIKGILRETGADLVLFDQDMKPSQQRSLEQALEARVIDRTQL
                     ILDIFARRARTTEGKLQVELAQLTYLLPRLTGKGKALSRLGGGIGTRGPGETKLETDR
                     RRIEARIARIRREVRSVAGRRAGQRRSRKESLIRTAALVGYTSVGKTTLFNRLAGESA
                     FTSPQLFATLDPLVRRTALPDGLPYFISDTVGFVKRLPPALLSSFKATLEEVLEAGLI
                     LHVIDLSAPNAEAQAASVEKTLAEIGARGIPTIRIYNKIDRLPDRRALLARNEIETES
                     VYLSAVTGEGLPALKSRIRALLFEGLRVFYIRVPHDREDIWASLEKRSLILKRREGAG
                     HLEARVMAEPSRLIDLAAYIVSGEPTW"
     gene            complement(10973..11905)
                     /gene="miaA"
                     /locus_tag="PKV64_02705"
     CDS             complement(10973..11905)
                     /gene="miaA"
                     /locus_tag="PKV64_02705"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPN16025.1"
                     /translation="MSSFERTLVFILGPTGSGKSAFALRLAEERGGEIISCDSMQVYR
                     GFDIGTDKPSAEDRARVPHHLIDVADAGTQFHAADFAALALEAIAAVRERERIPLVVG
                     GTGLYFKALEDGLFPGPGRDEAVRSALDAEAGERGPEVLWERLAAVDPAYAAKTGRRD
                     RIRIVRALEVFTLTGRPISEHFKRTEGLLGDERPVKIGLDRPRADLYRRIEERVDRMF
                     DRGLIEETRRLLAGGVPESAAPFKALGYKHVLRLLRGEIGPDEAAALTKIDTRHYAKR
                     QMTWFRKMPGVRWIQPDETDPASVLPAPGRAERR"
     gene            complement(11972..13075)
                     /locus_tag="PKV64_02710"
     CDS             complement(11972..13075)
                     /locus_tag="PKV64_02710"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HPN16026.1"
                     /translation="MSKERPLEGTLADIPFPRLLHDLWRQERTGRLTVRSEKGERIVF
                     FDKGHLLVAQSGSDPKDFLAALVRKKVLSTEQAARVERTASGQNLTVLKALGELGLLG
                     PIPLWSLAESFFARRMFTLFDREDGEYSFDPESVLAGGHRLGRLQTPDLILEGIRQMH
                     NDAMIARRLPSEEEPVRVSAPFFLPLLKFEPPERYALQILSGAPSLRRFQERSELGAR
                     ESRKILFAFLCLGILAPAEPSAAKKTPAVSPGAEQGRILEALNEKCAFIFKFVSKEIG
                     PVAVTVLNRALDEIRPSLGPLFQKMALQFDGKVEVDAALRLSVEHLPEDVFRTIVRGY
                     DEILTAEVLAVRKTLGPGQETALVRNLEKIGCA"
     gene            complement(13215..13925)
                     /locus_tag="PKV64_02715"
     CDS             complement(13215..13925)
                     /locus_tag="PKV64_02715"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HPN16027.1"
                     /translation="MTFAQRIQGVFFEPARTMADAAARPKPIAAILILILALTAVFSY
                     VAAPYSAKDSAAALKDNVKIQERMGKERFDEMMARMENPKPGGLAFRSLVVSPAFAGV
                     FLAIQALFLLIFGRMFSSEGAFLPVFSVLLHAKLIDGLLGNLVRAALILIRKSVIQTS
                     TSLALLAPGAEITSLTYLLLGQVDFFQLWMFGVLGFGLAAAFKIPLKKGMILSYAFWA
                     AKVAVNIALGLLSRSMLG"
     gene            complement(13927..14628)
                     /locus_tag="PKV64_02720"
     CDS             complement(13927..14628)
                     /locus_tag="PKV64_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN16028.1"
                     /translation="MSASPPLVIKVENLSKTYDLGKTQVKALRSVSFEIQKGEIVAIM
                     GPSGSGKSTLMNLIGCLDTPTSGSYTLNGQLASAMTENELAQVRNRDIGFVFQVFNLL
                     PRATAFHNVELPLIYKGTKKKERETKVRAALAEVEMLDRMDHRPPELSGGERQRVAIA
                     RALVNEPALILADEPTGNLDSRTGGEILKIFHKLHGEGHTIVLVTHDRSVAEQAGRII
                     HLRDGGIEREERKGA"
     gene            complement(14625..15860)
                     /locus_tag="PKV64_02725"
     CDS             complement(14625..15860)
                     /locus_tag="PKV64_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPN16029.1"
                     /translation="MTKKKKAVLIIAAAALIGFVILLNLSTRREKSIRATTEKAGRRD
                     LTSLISASGEIKPKKNINLSAQIPGRIIKIGVQEGQEVKRGDFLLGLDPSQYEANADH
                     DRAFIQSSQAERIKVEAQLAKNRSFYERQKNLYDNELISKEQLEQAKVQWEISEASLK
                     AIDFQIAQAQASLKSSLDNLSKTVFTAPIDGIITSLRVEEGEVAIIGTMNNPGTVLMT
                     IADLSVMEVEVEVDETDVVAVVLGQDAEVRVDAFPNEVFKGKVTEIGSSALQKSGLAS
                     STTQESKDFKVVVTLENPSPGLKPGLSAGADIITSRKEGVLAVPISALVVRDKPEPAG
                     GTSPKSREKTEEGVFVVEHGRARFVPVQKGIMGELEVEIASGLTESQTIISGPYDALR
                     TLKDGDLIKTDVRKEKTER"
     gene            16037..16816
                     /locus_tag="PKV64_02730"
     CDS             16037..16816
                     /locus_tag="PKV64_02730"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPN16030.1"
                     /translation="MTGYTSYLDAARDAAREAGDILRRDYRRAGPVSFKGEIDLVTET
                     DTASQALIHERLAARFPGHDFLGEEGLRKITGAGFRWIIDPLDGTTNFAHRFPWFCVS
                     IALEHDGALVAGIVFNPIMGEMFTAEAGGGAFLNGEPIRVSATGELGRSLVATGFPYD
                     VRESRTNMAAHNRFLLAAQAVRRCGSAALDLCYVACGRFEGFWELKLNPWDTAAGAVI
                     LAEAGGRLTDLRGRPADIYIPELCASNGLIHQAMLDILRET"
     gene            16813..17325
                     /locus_tag="PKV64_02735"
     CDS             16813..17325
                     /locus_tag="PKV64_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16031.1"
                     /translation="MMRALALAFALIRLAGGFPGGSAGDKASLRPGDFVLVKTLTAAG
                     ASGWTDTGLEVRAGEEFWFEASGTICLQTGNEEAYCGPEGIQVRTMQQPLPESNLGCL
                     VGRVLREVVIVEDPKTKEKTVREYGVAFPIGKAGPAVIPADGRLFLGPNENVTGDNDG
                     AFAVRVYRRG"
     gene            complement(17330..18679)
                     /gene="accC"
                     /locus_tag="PKV64_02740"
     CDS             complement(17330..18679)
                     /gene="accC"
                     /locus_tag="PKV64_02740"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HPN16032.1"
                     /translation="MFSKILIANRGEIALRIIRAAKELGIKTVAVFSEADRTSLHTML
                     ADEKFCIGPAKPQDSYLNIPNILSVAQITKADAIHPGYGFLAENARFAEICETEGITF
                     IGPPASIIRLMGDKNRARQEMMRAKVPVIPGSDKVLEDSDEAKKTAQKIGYPVIIKAS
                     AGGGGKGMRICRNANHLEEQFPIAQNEAKNAFGDPSLYLEKYIVGAHHIEIQVIGDKK
                     GNVVAFGERECSVQRKYQKVIEETPSPFIDEKLRRKMMKAVEDAAKYLGYENLGTFEF
                     LVDESRKFYFMETNTRVQVEHPITEMVYGINLVKTQIRTAAGEKVNFPFELKMKGHCI
                     ECRVNAEDPNTFAPSPGTIDFLVLPGGEGIRVDSGAYGGWVIPPYYDSLVAKIIAYAP
                     SRLLAIKKMQTALEMTTIVGIKTNIPLHLMILANSDFINGTYDTQFLEKFLARKNGD"
     gene            complement(18686..19147)
                     /gene="accB"
                     /locus_tag="PKV64_02745"
     CDS             complement(18686..19147)
                     /gene="accB"
                     /locus_tag="PKV64_02745"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013755498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HPN16033.1"
                     /translation="MDFEEINKIIKLLEEKNLSEFELEIEGFKIKVGRNVPVVVNNRP
                     SYVDLAPAAPNSIPFAGPVPEPPAEIKGDAHAVLSPMVGTFYRAPSPSSPPFVEVGDV
                     VKKKQTLCIVEAMKLMNEIESDADGVIKEIYVENGKPVEFGQKLFLIVPQA"
     gene            complement(19303..20559)
                     /gene="nagA"
                     /locus_tag="PKV64_02750"
     CDS             complement(19303..20559)
                     /gene="nagA"
                     /locus_tag="PKV64_02750"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HPN16034.1"
                     /translation="MPQTTKPARKMKTKAVKRGRPPVPYILIRAGVVLTPDKEIRRGA
                     VLIKGRRIVAVGRDKEIAAAVRKAHDQSDRFTMDVVEAPDLIAVPGLIDIHQHGGGGA
                     DYIDGTVEAARTVLETHVRGGTTSVLPTMMTASRPALKKALAAVDALRPRGKRGSLPA
                     TTEAGPDILGIHLEGPFISKKRHGAQPPGAVRRIDEAEIREYLRIFRTPIRLMTLAPE
                     LPGAPAFIKFLVRRGIIASAGHSDASFEEAIRGFDAGISHGTHLFNAMRGFFHREPGL
                     AGALLLNERASVELIADGRHLHPAAVFLVLQAKPADKVVFVTDASRNAGTKEAPLLTA
                     AGGLFGSNLRLLEALLKLMEWSGWSLQDVLPLATSNPARILGLEKRKGTLLPGADADI
                     VLLDYKFQVKDVWCAGRRASVFEKKQ"
     gene            complement(20559..21932)
                     /gene="glmM"
                     /locus_tag="PKV64_02755"
     CDS             complement(20559..21932)
                     /gene="glmM"
                     /locus_tag="PKV64_02755"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPN16035.1"
                     /translation="MKSATTLKISISGARGIIGDSLTPQLAAALAQAFGTYVGGGPVL
                     VGRDARSSGIMLAEAVVSGLLAVGCRPVDVGILPIPSFLYLTKEVRAAGGIAVTASHN
                     PREWNGLKFISRRGLYLTPQQTEEYLDIYHQGAFDFVAAGKFRTIEVLPRAADAHLRR
                     LLNRFDGEAIRRRRIRVVLDCNNGAGAVLGPRFLRELGCDVVPLACDPTGEFVHPSEP
                     TPENIAGVCRAVKTARADIGFVQDADADRLAIIDERGRPLGEEMTLVWAASRVLEKTK
                     GSIVCNMSSTRALDDLAARHGVKIFRTRIGEVNVVEKVLAARPRAVIGGEGNGGVIDP
                     EIHPCRDSFAAMGHVLDALAAGHKPVSALAGALPRYVLLKDRIEGSAERAHRLIRVLR
                     KRYEDKGTVNLLDGLKVDFPDHWIHLRPSNTEPVIRVLAEAKTEIRARRALAALRGEI
                     AAAWRKI"
     gene            complement(21929..23278)
                     /locus_tag="PKV64_02760"
     CDS             complement(21929..23278)
                     /locus_tag="PKV64_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16036.1"
                     /translation="MLKIGLSGVRGVVGPSMPPTMVMDFASAFGAFIDRKPVVVGRDT
                     RIHSPMLRAACVSALLSSGCDVLDLGVCPTPLLQKAVRRRKAGGGVSITAGHNDVSWN
                     ALAFIGPDGSPLNPFQGEEVLDYYHLGRFERAAVDHLGRLIPVPTAETADFYFKELGA
                     FIEAAAVRAARFRVVIDACAGAGAPFLARFADTLGFDLIPLNDEPNGFFPHDPEPRPR
                     NAAQCASVVRAAGAEAGFLLNSDAGRVSLVSETAEALSEEYTFPLVADSVLARCSGPV
                     VTNNSTSRMIEDVARARRCPLIRTRVGPAPIFLALAQEDGILAGEGSGGVAVPAFQPA
                     FDAFLTMGLVLETMAKTGQRLSALVDRLPRYHIVKEKVYCPASRIHGVISEIARMFRG
                     RDVDTGDGIRVEDKQGWVQVRASGTEPMLRVVAEDRSRERAREKVDEILRSLERMIP"
     gene            23403..24725
                     /gene="ffh"
                     /locus_tag="PKV64_02765"
     CDS             23403..24725
                     /gene="ffh"
                     /locus_tag="PKV64_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPN16037.1"
                     /translation="MFDQLSGRLQKAMKYLRGEAKVTEGNMSEALRQIRLAFLEADVN
                     YKVVKDFEARIKVRALDQTVLDSLTPAQQVIKIVRDELASVLGTTDKPLTFASAPPSI
                     FMLVGLQGSGKTTTCGKLAKWAAGKGRSPLLVSFDLKRAAAQDQLRTISGQLHLPFYE
                     MTAAAMAKPRSALADLLQYTRNRGYDVLLVDTAGRLHVDDELMDELRIVKDVLSPVET
                     IYVGDAMTGQDAVKSAKTFADRIGLTSIILTKLDGDARGGAALSIVAVTGIPIKFIGV
                     GEKFDKLEVFHPDRMASRILGMGDILSLIEKAEQETDLKEAEALAKRLRRQEFTFDDF
                     RSQLRQLRKMGSLEGVLGHLPQAGPFKNLAGARMDEARLRHFEAIIDSMTAAERTDPR
                     MINGSRRARIARGSGRPVSEVNQLLRQFQEMRSMMKKSIFQKMMSSLK"
     gene            24781..25092
                     /locus_tag="PKV64_02770"
     CDS             24781..25092
                     /locus_tag="PKV64_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007951696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HPN16038.1"
                     /translation="MYVVGVTIWIKPGFEEKFIEAVRMNHEKTRREPGNLRFDVLRHE
                     DEPGRFFLYEVYRSRDDFAAHQQTAHYLAWKETVADWMSRPREGIKHLSLFPEDRPAA
                     W"
     gene            25102..26280
                     /locus_tag="PKV64_02775"
     CDS             25102..26280
                     /locus_tag="PKV64_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPN16039.1"
                     /translation="MVFEWTAPARIVFGRGAAADIGRRAAEIGRRVLFIRTGALPATG
                     GTVEAARSALAAEGLEFLEAEVRGEPEVEAVDALTARVRPFEPEVILGLGGGSAIDTA
                     KAVAGLLAGGGEVLDYLEVIGRGRPFSRPGVPVIAVPTTAGTGAEATRNAVLASRALG
                     VKASLRGPALVPRLALVDPALTDTLPPAVTASTGLDALAQCLESYLALNARPWTDALA
                     LEGLNRAARSLVPAFRDGSVLEARDDMAVAALLSGICLANAGLGAVHGLAAALGGKFP
                     IPHGVACGRVLPGVLSENIRAMIAGGAGRTVPAKCLRLAEVFLPARRGSEKTRMAALG
                     DRLSAFVEELELPRLSAFGVTEADLPVLAEAAGRSSSIRQNPVALDEKTLIGILRAAL
                     "
     gene            26432..26686
                     /gene="rpsP"
                     /locus_tag="PKV64_02780"
     CDS             26432..26686
                     /gene="rpsP"
                     /locus_tag="PKV64_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009182675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPN16040.1"
                     /translation="MMLSLRLMRFGAKGQPAYRVVVIDSKKPRESKAKAIIGSYNPLT
                     DPAEIKIDLAKAKYWMDRGAKPSETVKSLLAKPQKATPKA"
     gene            26719..26952
                     /locus_tag="PKV64_02785"
     CDS             26719..26952
                     /locus_tag="PKV64_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPN16041.1"
                     /translation="MKELVELIAQALVDQPDKVVVSQIEGEQTTILELKVAPEDLGKV
                     IGKQGRTARAIRIILGAAGMKLKHRYNLEIIEK"
     gene            27055..27468
                     /gene="rimM"
                     /locus_tag="PKV64_02790"
     CDS             27055..27468
                     /gene="rimM"
                     /locus_tag="PKV64_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPN16042.1"
                     /translation="MFVRENDEFREYAVESLTPYKDAHTLKLRGVDSIPDALPLAGRE
                     IFALHEDVAPPAEDEFAVEDLAACRVVTKEGRPVGLVRGVWDTGGTPLLVLDPDGGGN
                     EIYIPLSRSICPVIDIEAKRIVIDPPDGLLDLNEI"
     gene            27458..28195
                     /gene="trmD"
                     /locus_tag="PKV64_02795"
     CDS             27458..28195
                     /gene="trmD"
                     /locus_tag="PKV64_02795"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPN16043.1"
                     /translation="MRFDIITIFPEMFPSVFAGGVIGKAVQRGRLDLRVHNLRDFTTD
                     KHQQVDDRPFGGEEGMVFKPEPVFAAVEAVRGSEDPPVYLLSPAGRRFDDRLAREIAL
                     QPRVVLICGRYEGVDERVALHLATDEISIGDYVVTGGELPAMIIVDAVARHIPGVVGK
                     EGSVRKESFAEGLLDYPQYTRPRDFRGMKAPEVLFSGDHDKIEAWRRRKALEKTRRRR
                     PDLFARLSLSPEDRMLLDEIDNERNEQ"
     gene            28192..28533
                     /gene="rplS"
                     /locus_tag="PKV64_02800"
     CDS             28192..28533
                     /gene="rplS"
                     /locus_tag="PKV64_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPN16044.1"
                     /translation="MSLVQVTEDKVMKKDLPPFAIGDTVKVHVLIKEGEKERVQIFQG
                     DVIAKKGRGMSETFTVRKVSFGIGVERIFPRQSPWIKKVEVVRSGRVRRAKLYYLRGL
                     KGKAAKLREER"
     gene            28542..29168
                     /locus_tag="PKV64_02805"
     CDS             28542..29168
                     /locus_tag="PKV64_02805"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPN16045.1"
                     /translation="MPDFEIERRHLEAGRTLIAGVDEAGRGALCGPVVAAAVILPFSW
                     FAGLPPDWVERLDDSKRLSPTRRERLARRIRQEASVGLGLAGNAEIDVLNILRASQEA
                     MRRAVAALPVPPDMVLVDGLAIRDFPRPQDAVVGGDRRSFSIAAASIVAKVSRDRIMI
                     RAARRFPGYGMERHKGYGTRAHFEALALRGPTPFHRMSFNLQYQPKLF"
     gene            29173..31506
                     /locus_tag="PKV64_02810"
     CDS             29173..31506
                     /locus_tag="PKV64_02810"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN16046.1"
                     /translation="MTIKASDRAKISERAGQFVKAGRIEEAVAEYQKLLDGSGQDVSI
                     GNIIGDLCLQLGQEDRAIELFMNNVETLERRGAYSQALAIAKKVNKIRPAEPDVMIRL
                     GDLYGQLGFSGEAREQYTQAADEYEKRNETKPLIALYEKRARLDRTDLVTRLKLARLY
                     LEERKGDQAQVVLNEAADLLFVRKEYGEAEKILLEALAVADGDYRTLTNLVRVYRELK
                     RPETSMEMLGRIVKKRGPRTDLLSLMALLHSEQGDKDKAAEIYGRILAEDAGNYDIRA
                     KLGIIEIERDRPDEALEIFDPLISHFLQKSDEDKAAGLLGLILMSGRMHLPTLERLAG
                     VFRGVGRTKDLEVVLRVLLAEYRSESRDNDRIRILRELYILLPMDAGIAEEAETLGIR
                     RELHQEAAEVAPEAGRPISEQDQEMLRLNLAKAELYLEQGLVRNARRILENLRLLYPD
                     DPRIQKKIESLRAAPSVIIDKKIPEIVEETARREIDIIGPAVEPGMVVPPVSAPPLDL
                     ELLISDFAQPLPPETPIHPAAETPSSRKKGPVFSVRSGPAASRTKPAPPPPPPAPQAE
                     APDEEAGSKVTSAEIFAGLDIGPFVASAAGSAAASQGYIDITDKIEEEIEALEVEFYK
                     QLKERTSVIEKDLVDIVQEFRRQVDIKVDQRNYESRYNLGLAFFEQNLYEEAIAEFEL
                     AAADPGRTADCWSLIGQCCVKKRNYPEARRRFEGAISLTPEESPERFALAYELASFYE
                     MISENEAALALYREISAWNPKFRNTAKRIKILEKIVS"
     gene            31524..32159
                     /locus_tag="PKV64_02815"
     CDS             31524..32159
                     /locus_tag="PKV64_02815"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPN16047.1"
                     /translation="MRIIDLSHLLAETMPVFPGDAAPRITEASTIDEHGFASKTLRLG
                     THTGTHVDAPAHFLAGGAALDRLPAARFAGPGCLVDVSRLAGPRIEIADLEGDRDRIA
                     ASEFVLLFSDWAKRWGSPAYFSGHPALSEKAARWLAGFKLKGVGVDAPSADVMDAARP
                     AVHRIFLSRGIVIIENLTGLRTLRGRAFLFACLPLNIAGGDGSPVRAVAIL"
     gene            complement(32366..33094)
                     /gene="ddpX"
                     /locus_tag="PKV64_02820"
     CDS             complement(32366..33094)
                     /gene="ddpX"
                     /locus_tag="PKV64_02820"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HPN16048.1"
                     /translation="MSTTSLDRGRMIPIHPGRRAASDADRGPRPLAAAAVVVLLAGAF
                     LSLGCRPGAAPDFAAAPPELVELAVAAPDIILDIKYATTDNFTGKAVYPIARCFLVKE
                     AAQALVQVQADLRTFGYGLKVFDGYRPLSVQKIFWSILPDERFVADPAKGSRHNRGYA
                     VDLTLVDAAGREVLMPTPFDDFTERAARDFMDLPREAIAHRALLELAMTNRGFIPYAP
                     EWWHFDFRGFEDKPVLDVPLDKMN"
     gene            complement(33259..34074)
                     /locus_tag="PKV64_02825"
     CDS             complement(33259..34074)
                     /locus_tag="PKV64_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16049.1"
                     /translation="MKKSVGIFLLAGLTFVFAAAVRAQSQNPAAASASMPASPAEPPE
                     AAPQAAAPPAPALRTELIKLKYADLETITILLRGYQSALGRVMPAGRAEKSIVVTDTP
                     EIVEKMISIVRDIDIKPAEIQYTVQIVQGSDAGEDTLKNDAVIRELRGILKYKSFVLL
                     DGTVIRVIDGETAQAKIGPKGEYEVWLRPKYTKDGTVETIQTEIRLGRQEWNPAVTVG
                     LVKKEEAPPAASRFNELIRTTLLLKAGEKTVVGVSKTDADRGLILILYGKAVK"
     gene            complement(34076..34696)
                     /locus_tag="PKV64_02830"
     CDS             complement(34076..34696)
                     /locus_tag="PKV64_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16050.1"
                     /translation="MNCRSARRRLSDPTGRIFSAGSKKRLEQHLRECASCRTEASAFR
                     AGIEALKHPAVFDDADFTEAEWRVAIRAAVAGGKTAKSDRFARFFRPSLGCVLGVLLV
                     GAAIFFGVRHIPWLIPKVENHPSRAAASAARPRIIKPNAPGTIPAETDSRAPAGGTVF
                     SAGRPSPFPSSAAPPPAGDVPSFTWISEETGLQIVWFINDSLNLEE"
     gene            complement(34693..35259)
                     /locus_tag="PKV64_02835"
     CDS             complement(34693..35259)
                     /locus_tag="PKV64_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPN16051.1"
                     /translation="MKEIELAARVLAGDSTAFEPLVLPYRKTLFGLAYKMTGNREDAL
                     EVAQETLLRAFRHLGKCDFERGFRNWLLQILVNVGRERAGRRRRETAWANSDFLLENA
                     GGGGPGPEEARGRQEIRERVLSALSALSARERDVFILRDLEGKSVRETAAIVRSSAIA
                     VRVHLSRARGKIRDRLAALDPSLQETSR"
     gene            complement(35437..36648)
                     /locus_tag="PKV64_02840"
     CDS             complement(35437..36648)
                     /locus_tag="PKV64_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16052.1"
                     /translation="MRFDRGLRLLIAGSFLLAAPAVFGENRAEQCTAAVISPAASAAG
                     GAVLWKNRDTAVLSNKVVFVRETPYSYAALINAEGASGRQAWAGLNSAGFAVMNTVAY
                     NLPQKSGELQDLEGVIMADALRTCATVEDFERLLRANLGPDLGARANFGVIDGSGRAC
                     LFEVHNHGFEKFEASAAPEKYLVVTNFARSGRAGAGAGYLRFDRAGRLFASLPPGRPI
                     DFQTILTRFSRDCGHALLRNPDPEEWKNIPAEPGLWIHARHTINRPDTSAAVVIVGKN
                     PEDPESSATMWVIPGEPLTAVAVPLWVEAGRSPEPLWKGENAPLWEESARIKTLIHPI
                     DEYDKNEYLLVSRLDNADGTGFLPKLLEAEEAILRETEAFLKTRPEPAEKADFQDRMA
                     ARALAAMKAVK"
     gene            36983..38485
                     /locus_tag="PKV64_02845"
     CDS             36983..38485
                     /locus_tag="PKV64_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HPN16053.1"
                     /translation="MNTESLLIAKGTEEIRLLPRMANRHGLITGATGTGKTVSLQVLA
                     ENFSRIGVPVFMADVKGDLSGVSRPGTETPKIGERLDKLGLRPFEFAGKPVVFWDIFG
                     DQGHPVRTTISDMGPLLLSRLFNLSETQSGVLSLVFKVADDNGLLLLDSKDLRAMLQY
                     CGDHADDFQTEYGRISPASIGAIQRSLLDLETQGGDRLFGEPALNLADFIQTDSQGRG
                     VINILAADRLMLQPKTYATFLLWMLAELYENMPEVGDLEKPKLVFFFDEAHLLFTDAP
                     AVLLQKIEQIVRLIRSKGVGVYFVTQNPLDVPDIALGQLGNRIQHALRAFTPRDQKAV
                     RAAATTFRANPKLDVEKTITELGVGEALVSFLDEKGRPGIVERAFILPPRSQIGPISA
                     DERRRIIGSSVLYGHYERQIDRESAYEHLKEKARQAEEAAARAARSPIPTGYPQGPSY
                     EPPRAPRPVGRPRDTFVESVGKSVARSLATSAGRQIIRGLLGSILGGQRR"
     gene            38583..39602
                     /locus_tag="PKV64_02850"
     CDS             38583..39602
                     /locus_tag="PKV64_02850"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPN16054.1"
                     /translation="MSEVNDGGNGGAGRDIVLLGRSASFARVRELIDRLKDLDTPVFI
                     SGESGTGKEVIAQAIHFRGARAGGRFVAVNGGAIPDNLLESELFGYAKGAFTGALRDK
                     PGLIEEAGGGTFFLDEVGDLGFPLQAKLLRVLQEREIRRIGETRTRPVDVRFISATNK
                     DLEREVSSGRFREDLYYRLRIVTIDVPPLRERPDDLELLLHHFIARYGEKIGRPRVYF
                     TPRVMETLLAYAWPGNVRELQNEVQRCLILCGNEGIVRVEHLSPRLNPGRSPGPAPES
                     FNFFEAKADFVRRFLGQALRRHENNKARTAEEIGLSRQALFKLLKKHRITAPGAAEGN
                     SPGAD"
     gene            39634..39927
                     /locus_tag="PKV64_02855"
     CDS             39634..39927
                     /locus_tag="PKV64_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16055.1"
                     /translation="MSPNEERNGDQAFEIACPHCGTVIWVDGRNRSILKTERAARKKE
                     SLDDLLAREKTKKDGLATKFEATASLQKQKKDRAKDAFAKAFAAAGEEPPAED"
     gene            complement(39935..41341)
                     /gene="nhaC"
                     /locus_tag="PKV64_02860"
     CDS             complement(39935..41341)
                     /gene="nhaC"
                     /locus_tag="PKV64_02860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HPN16056.1"
                     /translation="MTSPAPVKKPSLFLSLLPLILMAVLLGIGYGVYQIKPQVLLVAA
                     AALTGLLGWGLGYSWKEMEKGIVEGIAKAMPAILIMLAVGVMIGAWMAAGTIPMVVYY
                     GLKLISPKFFLVTACFVCSLTAVATGTSWGTIGTVGVAFMGIAMGLGLPLGPAAGAVV
                     CGAYFGDKMSPFSDIPNLAAVTAGADLFDHIKHMMWSAVPGWLLSLGVFFFFGFRSQA
                     QAVSSEVLTKITGTLRTHFTFNVWLLLPLVIVFALAFAKKPTIPGMLLSSLIAVLLAA
                     VFQKAPLADLAASLNTGYPAATGVPEVDRLLSRGGLMSMMETQLVAITAFCFGGIMQK
                     TGMLGVVLDKVMAGARKAGSIVLVTIGSCVVTALVTGSSYLAVILPGELLGPAFRKLR
                     LAPKNLSRIIDECGGIIVPLVPWSMAGVYVAGALGVPVLDYLPWAIGNYAALFILAVF
                     GLTGFTMTREDGGPRDEA"
BASE COUNT         7470 a        12777 c        13492 g         7718 t
ORIGIN      
        1 gagatcgtga aacgccttga gggcgaagat ggaggcgacc aggccctgtt ggttgtcctc
       61 gactccgcgg ccgtagatgc ggcccgagga aacccagggc ttgaatggat cgccctgcca
      121 gagggagatt tcgccgggcg gaacgacatc caggtgggtc atgatccaaa ccgtccgggc
      181 ggagtttcgg ccggggtagc gggcgacgat gttgggccgg tagccggccg gcgcgtcgag
      241 atcaggcgcc cggatgactt ggatatccgt gattcccgac tcccggagat aggcgatgag
      301 aaaatccgcc ttccgggctt cgccctcgcc gccggaggcg ggagaaatgg ccgggatgga
      361 ggcgagcttg atttggagat cgatcatctc gtcacggaaa aggtcgaggc gggcggcgat
      421 tttatcgaaa gacatccgcg tctcctgttt tcttttgagc ggattccttt ataacactcc
      481 cctccgccgg acgcaagcga agccgggcga ggcggacggc caaacgaaaa cgtccttaga
      541 tgggaggcgg cggatccgga gaaccggatc gcccgggcgg ccgcaagatc cggagcctat
      601 cggtttcacg gaagacggcg gaaggagggc cggccttaat gccggccgct cggtggatga
      661 ggcgcggcga tcgccggact gaaagccggg ggggtgcttt taacggccgg cctcaaagtc
      721 tcggctcatc gcgcccggct ccggcccgcc tttgattgac acccgcggag ggatgtcata
      781 catttacgaa aaatgcgcat cggattcgac gtccgtccct ttctccggca ggaaacggga
      841 gtgggcgtct acttcaagaa tctcctcttc gaactggcgg ccctcgaccg ggacaacgag
      901 tactgccttt tctcggcgtc ctacaaggat cgtttcccgc cggccaagat cccgccgttt
      961 gagaagctga cgtttgtcga taagcgctgg cccgtccggg cggtcaacgc ggcttgggac
     1021 atgttcggcc gcccgaggct cgaccggatc ttcggcggcc ggctggatat aacgcattcg
     1081 ccgacgccgc tgcgtctccc ggcgtcggga aaggcgatcg tcacggtctg cgacttgttc
     1141 tttttggaag agccggagaa ggccgaccgg gaagcccgaa cacggtttct ccgcaaaacc
     1201 ggacgttccc tccgttcggc cgacggcgtc atcacgatct ccgagtacag cgcccgggcg
     1261 atccgcgaac gattcggcct ggacgaggaa aaaataatgg tgacgccgct tgggctgaac
     1321 cgcttttttg aggacccggc cgccccggag gaaacggccg cggtccggcg ccggttcgac
     1381 ctccccggtg agtttctgct gttcgtgggt gcgctggagc cgaggaaaaa cgtcccggcc
     1441 ttaatcgagg ccctggccct ggttcatgag aggcatggac gcattccgct ggtcttggcc
     1501 ggccggtccg gcggcgacgg ggaaaacgtc gatcgggcga tcgagcggcg ggggctcggc
     1561 gcgtgggtcc gtcgaacggg gtatctcccg gacgtcgatc tccgggccct ctaccggacc
     1621 gcggccgcgc tcgtttttcc ttcgtcggcc gagggattcg gccttccctt gctcgaagcc
     1681 atggcctgcg gcctgccggc ggccgcctcc ggcgtgtcgg cgcttccgga aatcggcggc
     1741 gacgcggcgc tctactttaa tcccgaggat cccgaggaga tcgcccggac ggtcatccgc
     1801 ctcctggcgg acagtccggc gcgcgaatcc ttgaaaatcc gcggcctccg ccgggcggag
     1861 ctgttcccct ggcgccggac cgctgaagcc actctcggat tttaccggcg gacggcggga
     1921 aagacatgat ctccgtcctg ttcgtttctc attcggccga gctcaacggc gcggagcttt
     1981 ggctcttgga aaccctccgc cgcttggacc ggaggatgtt cgtcccggcc ttgatcatcc
     2041 cccgtcccgg gccgctcggt ccggcggccg aaaaagcggg cgtttccgtc gacgtcgtgc
     2101 cgatgaagtg gtgggtgacg gaaaaggcga aggtctggcg ccagcccgcg gcccggctcc
     2161 tcaaccggcg ggccgtcgcc cggatcggcc ggatcgcccg cgagcggaaa gccgggatca
     2221 tcttttccaa ttcggccgcg acgttcggcg gggccctggc cgcccgccgc ctgggaattc
     2281 cccatgtttg ggcgatccac gaaatcctcg ccggcgaccg cgcgtttctc cactccttcc
     2341 gcggatcccg ggccttgacc cggttcattc tcgatcgctc ggcccgggtg atcgtcaatt
     2401 ccgagtcaac ccggcgggcg tttcccccct ccggcaagat cgcccttgtc tacaacggcg
     2461 tggagatccg ttccgccgcg ggggagcggc gcggaatcgt ccgggccgaa ttcggcctgg
     2521 aggaaggaag ccggacgatc ggcgtcgtcg ggaagctgta tccgggcaag ggtcaaaagg
     2581 aggtccttct ggcgtttgcc cgtctcgccg gccggtatcc cgacctgaag ctgattttca
     2641 tcgggacggc ggccgatcgc cggtacgaac aagatcttcg ccgtctcgtc cgcctgaacg
     2701 atcttgcgcg gcgcgtcgtt tttgccggct ttcgtcccgc tctctcggag gtgctccaaa
     2761 acctgagcgt cgtcgtcgtc gcctcggtcg tcgattcgtt cggccgggcg gccctggaag
     2821 gaatggccgc gggcgtgccg gttctggcgg tcaaggccgg gggcctgacc gaaatcatcc
     2881 ggcccggccg aaacggcttc ctggctgaat cccgggaccc cgaagttctg gccgcggcgc
     2941 tcgccgagat cctcgatcat cccgaacgtt ggccggccgt cgttgaaaac ggcttccgga
     3001 cagtccggga ggatttctcg atcgaaaaac aggtccaagg ggtcacccgg atcctgctgg
     3061 aggtctgcgg gcggacgatt ctttgcccgt ctccgccgga agccggctcg aacggacctc
     3121 ttttcgcttc tttccccggc ggggggaagg gagacggccg cggatgagca ctcccctcgt
     3181 ttccgtcatc ctcgtttatt tcaacgaccg tctccacttg gcttcctgcc tggagtcggt
     3241 cgaggcggcc gggcgggacg tcgccctgga ggcgatcctc gtggacaacg actcgaccga
     3301 cgggggtctc gaggacgccg ccgctttccc ttccgtccgg atcatccgga acggggacaa
     3361 tcccggtttc ggagcggcca acaaccgcgg cgtccgggag tcaagggggg aattcctgct
     3421 ctttttgaac accgatgcgg ttcttcagcc gggggcagcg gcggctctcc tggaggccgt
     3481 gagggcggat gagcggacgg ccgcggccgg gcccctcttg ttctccggcg gcgggaaggt
     3541 ccaggtttcc tttggaggcc gggtggattt cgtcggccag ctcgtccaga aattcatcaa
     3601 gaatcccctg acggcccggg cgcttcggcg ccggccgcgc ggggcggagg ccggctggct
     3661 gagcgcggcc tgcctgtttt gccgacgcgg ggcgtttgaa gcggccggcg gctttgacga
     3721 gagctttttc ctctatttcg aggatatcga tctttgcttc cgcttccgaa aggccggcgg
     3781 ccggctgcgt ttcgtccccg aagcgcgggt cttccacgcg ggcggggcga cgacgggtcg
     3841 acgtccccgc gaatcgcgcc tggagtaccg gaaaagccag atgctctttt atcgaaagca
     3901 cgcttcgccg gcgtccctcc gcctgctgcg gctttccctt cggattcatc tcggccttca
     3961 gagaatcctc gggcgtttcc ggggcgagga tgggagggcc atgcgggcgg cgtaccggaa
     4021 gctgctgaaa ccggaaaggg gaatcccgtg agacgcctga aagtcctgga aatgatcgac
     4081 aagccgtcgc tcggcggcgg acaggccgtt ttactggcct tggcccggaa tctcaataga
     4141 aaccgtttcg atgtctccgt ttgcgccgga ggcggaggtc cgctggaaga ggaggtccgg
     4201 cgggccggga tgacgttcca tccggtggaa ttcagaaaga atccggcggc cggccccggg
     4261 cggacgctca tccggcttct ggcctcccaa ggcttcgacc tgcttcacac ccacggcggc
     4321 gtggccggcc tctacgggcg gagagccgcc cgccgggccg gaactcccgt cgtcgtccac
     4381 acgattcacg gcatccatta ccttcattac cgcaatccgc tgctccgccg ggcggccgtc
     4441 ctcctggagc ggatcttgag ccggtcgacc tcggccgtcg tctgcgtttc cgaggccgac
     4501 ctggcccaag cccgacggca ccgtctcgct ccggacggcc ggcttcgtct ggtccgcaac
     4561 ggcgtcccct ccatcgaggc ggcatccgat ccggaccgcg tctcgagggg aagagctctt
     4621 tgttcggaac tgaaactccg ggcgccggtg atcgggacgg tcgcccggct tcatcgccag
     4681 aagggcgtgg aatatttcgt cgaggcggcg ccggatatcc tccgccggca tccggaggcg
     4741 ttttttctcg ttgtcggcgg cggacctctt gaaagccgcg tccggaaagg gatcgagcgg
     4801 ctcggcctga ccggccgctt catcctgtcg ggggagcggc cggacgcctt cgacctgctg
     4861 tcggcgttcg acatcttcgt ccttccctcg ctctgggagg gcttgcccct ggtcctcatc
     4921 gaggccgccg gcttcggaaa acctatcatc gccaccgata tcgacgggtc gcgggaaatc
     4981 atccgagacg gggagaccgg cctgcttgtt ccgccggccg acccggccgc cctggccgag
     5041 gccgtcgtcc gcctgctgga ggatgaggcc ttggcccggc gattggcgga acgagcccga
     5101 aacgaaatcc cggcggcgtt cagcctggag cggatggtcg ccgagaccga gcgcctctat
     5161 ctggacctgg cggccgcgaa cgggctataa tattcattcc catgaaaaac gcgcgggtcg
     5221 cccttatcca tgactggctc accgggcgcc ggggcgggga aaaagtcctc gaggttcttg
     5281 cggagatttt cccggcggcg ccgatctaca ccctcttcca ttttcccgga agtcaaatcc
     5341 ccgagattga aaaacgcgtc atccggacga gcttcctcca gggaatgccc gctcttcgcc
     5401 ggcattaccg ctggtatctc ccccttttcc ccctcgccgc cgaatcgttc gatctccagg
     5461 gattcgacct ggtcgtctcc agctctcatt gcgcggccaa ggggatcatt ccgaagccgg
     5521 aagccctcca tgtgtcttat atccattctc ccgtccgcta cgcctggaac cagtatgccg
     5581 cctatttcgg tcccggcaag ctcggttttc cggccaaata catcgtcccg cccatgatcc
     5641 acggcttgcg gaactgggac gtgacgtcgg cggcccgggt ggaccggttc atcgccaatt
     5701 cccgggcggt cgcctcccgg atcgagaaat attatcgccg gccggccgag gtcgttcatc
     5761 ctccggtcga cacggaattt ttcgtcccgc ccgattccgg gcccgaacgg aagtatttcc
     5821 tggccgtgac ggctcttgtc ccctacaagc ggatcgacct ggccgtggcc gcctgcaacc
     5881 tgggcaagtt tccgttgaaa gtcgtcgggg acggtccgga gatgcgctcc ctccgggcgg
     5941 cggccggccc gacggtcgag ttcctgggat ccgtgagcgg cgacgatctc cgccggctct
     6001 accaggaggc gatcgcctat ctcctgcccg gcgaggagga tttcggcata agcgcggtcg
     6061 agtcccaggc ttgcggaacg ccggtgatcg ctctggcccg ggggggagcg ttggaaacgg
     6121 tccggccggg cgagaccggc ttgttgttcg aggagccgac ccccgccgcc ttggccgccg
     6181 ttcttgacaa atgccggggg ttgaatttta ataaggcctc cctttgcgct catgcccgga
     6241 ccttttcccg ggacggtttc aaggcgaaga tggaaacccg cctggccgcc ctatggcggg
     6301 aattcaagga accaaggtga tcagccggta ccaggccaga ctgtcggctc ttttcgtggc
     6361 cagtgatgtg ctggccatca cggcgacctt catttattcc tatgccttcc gttttcattc
     6421 ctatttcatc ccggtggatc cggccaaggg gatcccgacc tggcgctctt acttgttcgt
     6481 tctgcccttg ttcctggccg ttcacttggc catttttttt ctccagggtt tctacaaaag
     6541 ccgcctccgg cggaccaaga tcgatgattt cttctccatc acgatcaacg ccgtcctgac
     6601 gctgattgtc gtgatggccg tctccggcta tctgaacgcc tattccggag gcacggcccc
     6661 gcttttccgg gtgaacatcg tcaagctctc ccatctcttt ttggccgttt atttcatcgg
     6721 cgtgatcttc acgatttcgt tcttccgcaa ccaaatctat ttcttcatga agcggcggtt
     6781 cgcccgcggc ctcaacctcc gcagcgtcct ggtcgtcgga gcgggggaaa tgggcgtggc
     6841 cgtcgcgcag aagctgttcc aatacaagga tctcggcttc gtggtcaagg gctttctcga
     6901 caaccacaaa ccggccggga aaatcatcca ggtcgacggg ggaatccccg ttctcgggcc
     6961 ggtctccgac ctcggcaagg tcatcgaagc ccaggggatc caggaagtct acgtcgccct
     7021 cgagctggaa aattacccgg aaatgctcga agccctccaa gtcgtcaaca agtatccggt
     7081 caaccttcgt ctcatccccg acttgttcca gctcctcacc ctgaaagcca acgtccagga
     7141 cctggacggc ttcccggtca tctcggtcga tgaagtcccc ctccgcgggg cccggcgggt
     7201 gctgaagcgg ctgttcgaca tcgttgtttc cggattggcc ttgatcgtcc tgtctccgct
     7261 gttcctcatc gtcgcgatcc tgatcaagat gacgtcccgg gggccggtgt tttacagcca
     7321 ggaacggttc ggcctggacg gcaagcgatt caagatccat aagttccgga cgatggtcca
     7381 aaacgccgaa aaggccacgg gaccggtcat gtgcgcgccc gatgacgcgc ggatcacgaa
     7441 agtcggacgg cttctgcggc ggtacagcat cgacgagctg ccccagctgg tcaacatcct
     7501 ccggggagac atgagcttgg tcggcccgcg cccggaacgg cccgagttcg tcaaggaatt
     7561 caccgacagc attccgaaat acatgctccg ccacaagatg aaatcaggat tgacgggctg
     7621 ggcccaggtc cacggcctcc ggcaggggac gtccatcgag aaacggctcg agcacgactt
     7681 ctattacatg cggaattggt cctttaccct ggacctgaag atcatctgga tgacctttcg
     7741 gcggggattc atcgacaaat cgatgacttg agaacgatca gctttcctcg gcggcgcgac
     7801 gctcccggag aaggcggagg ccgcgttgat aatagagaat ccacgaggcc agggtgaacc
     7861 cggccgtgag ggcatagaac acgagcatcc acccggcttg ttttcccagg tggtttgtca
     7921 gcaggaccag aaagaccgtc cccacttgga aggccgtcgt taatttcccg agaaagaccg
     7981 gcaggacggt tttaactccc atgattaaat acgcccagag aacgccgccg acgatcatca
     8041 gatcgcgggc gaagacgatg atcgtcagcc agagggggac tcggttggga ccgccgatcc
     8101 cggggagagt caggagaata taggaagcgg cggtgaggag cttgtcgccg gccggatcca
     8161 gaattgtgcc caggcggctc ctctggtgga gatgacgggc gatgaatccg tcgaaaaggt
     8221 cggaaatcgc ggcgatgaag aaaatgacca ggggctcgat ggtttgccgc tcgaggaaag
     8281 tccagatgaa cagcggaacc aggaggatcc ggaaggcgct gagaaggttg gggacggtca
     8341 gggccgagga aacccagtct tccgcttccg ggcgatccgt ccggctcatt tcccgggttc
     8401 tttcgccagg ccggccagca ggtccaagat tcggatcgcc tccgcgccgg gcacaggttg
     8461 gtcgggccgg aacgtgcgat cggagaagat atccataagt tggaatgaaa cggccgcaat
     8521 gatcgccggg gcgtagggat ggtcggcggg gacgtcggct agattgatcc gctcgggcgg
     8581 gatttggacg acgatcgtcc ggcctttttt ccgcaggtat tcgaccaggt ggacgaccgt
     8641 ttcggccatc tcggcccggt tcatcgtttt tttcggctgg aacgtgtgat tggaatagat
     8701 ctccatgatc ccggaggcgg cgaccttgac gatgaacgtc cgggcccagg aggtcgaaat
     8761 gtcgacaaga acgggcggcc gaacggctac tccgatccag atgtcattga attttgcccc
     8821 gatcagggcg gccacatcct ccttggtcac ggcctccagg ccggggattt cccggtattc
     8881 gtcggggaga tcgacgactc ccagccgggt tttcagggct tccaggcgtt cggcgaccgt
     8941 tttatcggaa ggatcgagcg cctgaagctt ctggaaggcg tccaggctcc gggaaaattg
     9001 gttcatctgg tagagcgtct cggcgtattc gcggagaacg gttcggtcca ggggattgtt
     9061 ctcggcggcc aggccgaggt gaaacaaagc ccgcgggtag tctttttccc gggtgaacag
     9121 acgggccagg gaaacgtgag cgatctgaag cttcggggcg tattcgagga ccttgaggaa
     9181 ggcctccttg gccgcggccg tctttcccgt gcgggcggcc gcgtcggcct cgaggatcag
     9241 gctttcgacg atcgccgtcc gcagctcgtc ggcctctctc cgggcgaagg cgttctccgg
     9301 atcccgtttc agcacttcga ggtaggatcg cagggcgtcc tccctgcggc cccatcgccg
     9361 gtagacctgt cccaaaccga gctgggacgt gacggcgtcc ggagagaccg taagggagcc
     9421 gacaaaatcg gcctcggccg cggtcaggtc gttgcggagg agggcgatat aacccatcgc
     9481 cgtccagtag aacggatggc tttccccgag gcggagaacc gctttttcgg ccttgtccgc
     9541 ccgctccagc cggaggagat tccagacctt gtcgacggcg atccggtcgt ccagagagag
     9601 agccgcggag atctcggccg gaggattttc caggtaaatg gtcggctcgg cgggcgggct
     9661 tatggtcgcg caagcggcgg cggcgaggac ggccgccagt gttccagccg ttttctttac
     9721 cacgtcggtt ctcctgagac gatataggcc gctagatcaa tgaggcgaga cggctcggcc
     9781 atgacccggg cctcgagatg accggcccct tcccgtcgct tcagaatcag cgagcgtttt
     9841 tcgagtgaag cccaaatatc ctcccggtca tgagggacgc ggatataaaa tacccggagg
     9901 ccttcgaaaa gtaaagcgcg gatgcggctt ttcagggccg gaaggccttc ccccgtcacc
     9961 gcggatagat agacgctttc ggtttcgatc tcgttgcggg cgagaagagc gcgacggtcg
    10021 gggagacggt cgattttatt gtagatccgg atcgtcggaa tgccccgggc cccgatttcg
    10081 gccaaggttt tctccacgga ggcggcctgc gcctcggcgt tcggcgcgga aagatcgatc
    10141 acatgaagga tcaggccggc ttcgaggact tcctcgaggg ttgccttgaa cgaagagagg
    10201 agggccgggg ggagacgctt gacgaaaccg accgtgtcgc tgatgaagta gggcaggccg
    10261 tcggggagcg cggtccggcg gacaagggga tccaaggtgg cgaacaattg gggcgaggtg
    10321 aacgccgatt ctcccgccag gcggttgaac agcgtcgttt tcccgacgct cgtgtatccg
    10381 acgagggcgg ccgtccggat caagctttcc ttccggcttc ggcgctggcc ggcccggcgg
    10441 ccggcgaccg aacggacttc gcggcggatc cgggcgatcc gggcttcgat ccggcggcga
    10501 tcggtttcca gcttcgtttc gcccggcccg cgggttccga tgccgccgcc caagcgcgag
    10561 agagcttttc cttttcccgt caggcgggga agaagatagg tcaactgcgc cagctcgacc
    10621 tggagcttgc cctccgttgt ccgggctctc cgggcgaaga tgtccaggat cagctgggtc
    10681 cggtcgatca cccgagcttc cagggcttgt tccaggcttc gctgctggga gggcttcata
    10741 tcctggtcga agaggaccag gtcggcgccc gtttcccgga ggattccctt gatctcttcg
    10801 acttttcccc cgccgatgaa agtccggggg tcgggcgaag cccgggtttg aaaaaccttc
    10861 cgggcgatcc gggcgcccgc cgcccgggcc aggtcttcca actcggccat cgattcgatc
    10921 gcctccgaac ggtctctgga cgaggtcatc agatggacga gaacggtcct gttcatcgcc
    10981 gctccgcccg tcccggcgcc ggcaaaaccg acgcgggatc ggtttcatcc ggttggatcc
    11041 agcggacgcc gggcattttc cgaaaccagg tcatctgccg cttggcgtaa tggcgggtgt
    11101 cgattttggt gagggcggcc gcttcgtccg ggccgatttc cccccgaagg aggcggagga
    11161 cgtgcttgta gccgagggct ttgaacggcg cggccgattc cggaacgccg ccggccagaa
    11221 gtcggcgcgt ttcctcgatc aaaccgcggt cgaacatccg gtcaacccgc tcctcgatcc
    11281 tccggtagag atcggcccgg ggacggtcca agccgatttt aacggggcgt tcgtccccga
    11341 gaagtccttc ggtccgtttg aagtgctcgg agatcggccg cccggtgagg gtgaagactt
    11401 ccagcgcccg gacgatgcgg atccggtcgc gccggccggt ctttgccgcg taggccggat
    11461 cgacggcggc caggcgctcc cagaggactt ccggcccgcg ctcgccggct tcggcgtcca
    11521 gggctgatcg gacggcctcg tcccgtcccg gacccgggaa aagaccgtcc tcgagggctt
    11581 tgaaatacag gcccgtcccg ccgacgacga gggggatccg ttcccgttcg cggacggccg
    11641 cgatcgcctc caaggcaagg gcggcgaaat cggcggcgtg gaattgcgtc ccagcgtcgg
    11701 ccacgtcgat gaggtgatgg ggcacgcggg ccctgtcctc ggccgagggc ttgtccgtgc
    11761 cgatatcgaa tccgcggtag acctgcatgg aatcgcagct gatgatctcg cccccgcgct
    11821 cttccgccag gcggagggcg aaggcgcttt tcccggaccc cgtcgggccg aggatgaaga
    11881 cgagggttcg ttcaaaggat gacaaagcgg atgatgaaga cggccgtcca tacggtcgtc
    11941 aggagaagaa gggcgaagac ctgccggcgg ctcaagcgca tccgattttc tccagattcc
    12001 ggacgagcgc ggtttcctgg cccggcccca gggttttccg gaccgccaac acctcggctg
    12061 tcaggatttc gtcatagcct cggacgatgg tccggaacac gtcctcgggc aaatgctcga
    12121 cgctcaagcg aagggcggcg tccacttcca cctttccgtc gaactgaagg gccatttttt
    12181 ggaaaagcgg ccccagggaa gggcggatct cgtcgagcgc ccggtttaaa acggtgacgg
    12241 cgaccgggcc gatttccttg gaaacgaatt tgaaaatgaa ggcgcatttt tcattgaggg
    12301 cttcaaggat tcgaccttgt tccgccccgg gcgagacggc cggagttttt ttagccgccg
    12361 agggttcggc cggagccaga atgcccaggc aaaggaaggc gaataggatt ttccggcttt
    12421 cccgggcccc gagctcgctc cgctcctgga acctgcggag ggagggagcg ccgctgagga
    12481 tctggagggc gtagcgctcg ggcggctcga atttcaggag ggggaggaaa aacggggcgg
    12541 aaacgcggac cggctcttcc tcgctcggca ggcggcgggc gatcatcgcg tcgttgtgca
    12601 tctgccggat cccctcgaga atcaggtcgg gggtctggag ccggccgaga cggtggccgc
    12661 ccgcgagcac ggactcggga tcgaaagaat actccccgtc ttcccggtcg aagagggtga
    12721 acatccggcg ggcgaaaaag gattcggcca ggctccaaag ggggatcggg ccgagaagtc
    12781 ccaactcgcc caaagccttg agaacggtga gattctgacc cgaagccgtc cgttcgacgc
    12841 gggccgcttg ttcggtcgac agaacttttt tccggaccaa ggcggctaaa aaatccttgg
    12901 gatcagatcc ggattgggcg acgagaaggt gtcccttgtc gaaaaagacg attcgttccc
    12961 ccttttcgct ccggacggtc agacgcccgg ttcgttcctg tctccagaga tcgtgcaaaa
    13021 ggcggggaaa cgggatatcc gccagcgttc cttccagcgg gcgttctttc gacatgcctt
    13081 taatttatcg tccggcggcg gggctgtcaa cgcagtccaa agagggaagg gaaacgccga
    13141 ttcatcgcgt ttccccggtt ttgctccgcc gggcgcggaa ataaggtgaa ttagcctgtg
    13201 tctccctatc gggctcagcc caacatggac cggctgagga ggccgagggc gatattcacc
    13261 gcgactttgg ccgcccagaa ggcgtaggaa agaatcattc cttttttcaa ggggattttg
    13321 aacgccgcgg cgagaccgaa tcccagcacg ccgaacatcc acagctggaa gaaatcgacc
    13381 tggccgagga ggaggtaggt caaagacgtg atttcagcgc cgggggctag aagggccagg
    13441 ctggtcgatg tttgaatcac cgatttccgg atgaggatca gggcggctcg gacaagattg
    13501 ccgagaagcc cgtcgatcag cttggcgtgg agaagaacgg agaaaacggg aaggaaggcg
    13561 ccctccgacg aaaacatccg gccgaaaatg aggagaaaca gggcttggat cgcgaggaac
    13621 acgccggcga aggccggcga gacgacgagc gagcgaaagg ccagcccgcc cggcttcgga
    13681 ttttccatcc gggccatcat ctcgtcgaac cgctccttgc ccatccgttc ttggatcttg
    13741 acattgtcct tcagggccgc ggccgagtcc ttggcgctgt acggcgccgc cacataagag
    13801 aaaacggccg tcagggcaag gatgagaatc aggatcgcgg cgatcggttt gggccgggcg
    13861 gccgcgtcgg ccatcgtccg ggccggttcg aaaaaaacgc cttgaatccg ctgggcgaac
    13921 gtcatgtcag gctcctttcc gctcctcgcg ctcaattccg ccgtcacgaa gatggatgat
    13981 ccggccggct tgttcggcca cgcttcggtc atgggtgacg aggacgatcg tgtgcccttc
    14041 gccgtgaagc ttgtggaaga ttttcaggat ttcgccgccg gtccgggaat caagattgcc
    14101 ggtcggctca tcggctaaaa tcagggccgg ttcgttgacg agagcccggg cgatcgcgac
    14161 cctctggcgc tctccgcctg aaagctcggg ggggcggtgg tccatccggt cgagcatctc
    14221 gacctcggcc aaggcggccc ggaccttcgt ttcccgttct tttttctttg ttcctttgta
    14281 gatcaggggc agttcgacat tatggaacgc cgtggcccgc ggaagaagat tgaacacctg
    14341 gaaaacgaat ccgatgtccc ggttgcggac ttgggccagc tcgttttcgg tcatggccga
    14401 ggccaactgg ccgttcaatg tgtaggagcc gctcgtcggc gtgtcgaggc agccgatcag
    14461 attcataaga gtcgattttc cggagccgga aggccccatg atggccacga tctccccctt
    14521 ttggatctcg aaggacacgc tccggagcgc cttgacttgg gtcttgccca ggtcataagt
    14581 cttggagaga ttctcgacct tgatcaccag gggaggggaa gcgctcatct ttcggttttt
    14641 tccttccgaa cgtccgtctt gatcaagtcc ccgtccttga gggtgcggag cgcgtcgtac
    14701 ggtccggaga tgatcgtctg gctttcggtc aggccggaag cgatttcgac ctccagctcg
    14761 cccatgattc ctttctggac ggggacgaac cgggcccgtc cgtgctcgac cacgaacacg
    14821 ccttcctccg tcttttcccg ggattttgga gacgtcccgc cggcgggctc gggcttgtcc
    14881 cggacgacga gggccgagat cggcacggcc agcacgccct ctttccggga ggtgatgatg
    14941 tcggcgccgg ccgataagcc gggcttgagg ccgggggagg gattctccag ggtcacgact
    15001 actttgaagt ctttggattc ctgggtggtc gacgaagcca gcccggattt ctgcagcgcc
    15061 gaggagccga tctcggtcac ttttcccttg aagacctcgt tggggaaggc gtcgacccgg
    15121 acttcggcgt cttggcccag gacgaccgcg acgacatcgg tttcgtccac ttccacttcg
    15181 acctccatga ccgaaagatc ggcgatggtc atgaggaccg tcccgggatt gttcatcgtc
    15241 cctatgatgg cgacttcgcc ctcctccacc cggaggctcg tgatgatgcc gtcgatcggg
    15301 gcggtaaaga ccgtcttgga gagattgtcc agggagcttt tcagggaggc ctgggcttgg
    15361 gcgatctgaa aatcgatggc tttcagcgag gcctcggaga tttcccattg aaccttggcc
    15421 tgttccagct gctctttgga gatcagctcg ttgtcgtaaa ggtttttttg gcgttcatag
    15481 aagctccggt tcttggccag ctgggcctcg accttgatcc gctcggcctg ggagctctgg
    15541 atgaaggccc gatcgtggtc ggcgttggct tcatactggg agggatccag tccgaggaga
    15601 aagtcgccgc gcttgacctc ctgcccttcc tgaactccga tcttgatgat tcggccgggg
    15661 atttgggccg acaggttgat gttctttttc ggcttgattt cccccgacgc ggagatgagg
    15721 gatgtcaggt cccggcggcc ggctttttcg gttgtcgccc ggatggattt ttcccttcgg
    15781 gtggagagat tgaggaggat gacgaagccg atgagggccg cggcggcgat gatcaagacg
    15841 gcttttttct tttttgtcat ggattgagcc ttgtccgaat aaaatcaccc tttcatacga
    15901 tggagcggct cgaaagttca gaaaataagc gcggcgggat cgatttccgc cgccgtcccg
    15961 gaggccgctc cgccggccgg cgggaaaagg ggaaacgggt tgaaagacgc gtctccctgg
    16021 tctatgatag ccggacatga ccggttacac gtcctacctg gatgccgccc gggatgccgc
    16081 ccgcgaggcg ggggacatcc tcaggcgcga ttaccgccgg gccggccccg tgtccttcaa
    16141 gggggagatc gatctcgtca cggaaaccga caccgcctcc caggccttga tccatgagcg
    16201 gctggcggcc cgctttcccg gccatgattt cctcggagag gaagggctcc gaaagatcac
    16261 cggggccggc ttccgctgga tcatcgatcc gctcgacggg accacgaatt tcgcccaccg
    16321 gttcccttgg ttctgcgtct ccatcgccct ggagcacgac ggggcgctcg tcgccggaat
    16381 cgtcttcaac ccgatcatgg gcgagatgtt cacagccgag gccggcggag gggctttcct
    16441 taacggcgag cccatccggg tctcggcgac cggggagctc ggccgaagcc tggtggcgac
    16501 cggattccct tacgacgtcc gtgagtcacg gaccaacatg gccgcccata accgttttct
    16561 cctggccgcc caggccgtcc ggcgatgcgg atcggccgcc ttggacctct gctacgtcgc
    16621 gtgcgggcgg tttgagggat tctgggaatt aaagctcaac ccgtgggata cggccgccgg
    16681 ggcggtgatc ctggccgaag ccggcgggcg gctcaccgat ttgcggggcc gcccggcgga
    16741 catctacatc cccgagctct gcgccagcaa cgggctcatc catcaagcga tgctcgacat
    16801 tctccgggaa acatgatgcg cgccttggcc ctcgccttcg ccctgatccg cctggccggc
    16861 gggtttcccg ggggatcggc gggggataaa gcctctctcc ggccgggcga tttcgtcctg
    16921 gtgaagacgc tgacggcggc gggagcgtcc ggctggacgg ataccggcct cgaggtccgg
    16981 gccggggagg aattctggtt tgaggcgtcc ggcacgatct gcctgcagac cggcaacgag
    17041 gaagcctatt gcggtcctga aggcatccag gtgcggacga tgcagcagcc gctgccggaa
    17101 agcaacctcg gttgtcttgt cggccgggtc ctacgggaag tcgtgatcgt cgaggatccg
    17161 aaaacgaagg aaaaaacggt ccgggaatac ggcgtggctt tccccatcgg gaaagccggc
    17221 ccggccgtga tcccggccga cggccgcctc ttcctcggtc ccaatgagaa cgtgaccggc
    17281 gacaacgacg gcgcctttgc cgtccgtgtc taccgccggg gctgaacgat caatcgccgt
    17341 tcttgcgggc caggaatttt tccagaaact gcgtgtcgta cgttccgttg atgaaatccg
    17401 aattggccag gatcatcaag tggaggggga tgttcgtctt gatcccgacg atcgtcgtca
    17461 tttccaaggc ggtctgcatc ttcttgatgg ccaggagccg cgacggcgcg taagcgatga
    17521 tcttggccac gagcgagtcg taatagggcg gaatgaccca tccgccgtag gcccccgaat
    17581 ccacccggat gccttcccct ccggggagga cgaggaaatc aatcgtcccc ggcgacgggg
    17641 cgaacgtgtt cggatcctcg gcgttgaccc ggcattcgat gcagtgaccc ttcatcttga
    17701 gctcgaaggg gaaattgacc ttttcccccg cggccgtccg gatctgggtc ttgaccaggt
    17761 tgatcccgta gaccatttcc gtgatcggat gctcgacttg gacccgggtg ttggtctcca
    17821 tgaaatagaa tttccggctc tcgtcgacca ggaactcaaa ggtccccaag ttttcgtatc
    17881 cgagatactt ggccgcgtcc tcgaccgcct tcatcatttt ccggcggagc ttctcgtcga
    17941 tgaagggcga aggcgtttcc tcgatgactt tctgatactt ccgctgaacg gagcattccc
    18001 gctcgccgaa ggcgacgacg ttgcctttct tgtcgccgat gacctggatc tcgatgtggt
    18061 gggctccgac gatgtacttc tcgagataca gggacggatc cccgaaagcg tttttggcct
    18121 cgttctgagc gatgggaaac tgctcttcga ggtggttggc gttgcggcag atccgcatgc
    18181 ccttcccgcc ccctccggcc gaggccttga taatgacggg atagccgatt ttttgggcgg
    18241 tttttttcgc ctcgtcggaa tcctcgagga ccttgtcgct tcccggaatg accggaacct
    18301 tggcccgcat catctcctgg cgggcccgat tcttgtcgcc cataagacgg atgatcgagg
    18361 ccgggggacc gatgaaggtg atcccctcgg tctcgcagat ctcggcaaac cgggcgtttt
    18421 cggccaggaa tccgtacccg gggtggatgg cgtcggcctt ggtgatttgg gcgacgctga
    18481 ggatgttggg aatattgaga tagctgtcct ggggcttggc cggcccgatg cagaatttct
    18541 cgtcggccag catggtgtgg agcgacgtcc ggtctgcctc ggaaaaaacg gccacggttt
    18601 tgatgcccag ctccttggcc gcgcggatga tgcggagggc gatttccccg cggttggcga
    18661 taaggatttt cgaaaacatc gcgccttacg cctgcgggac gatcaggaac agcttctgac
    18721 cgaattcgac gggcttgccg ttctcgacat agatttcctt gatgacgccg tcggcatcgg
    18781 attcgatctc gttcatgagc ttcatcgctt caacgatgca cagggtttgt ttctttttga
    18841 cgacgtctcc gacctcgaca aacggcgggg aactggggga aggcgcccgg tagaacgtcc
    18901 cgaccatggg ggaaagaact gcatgggcgt ctcccttgat ttcggcggga ggttccggaa
    18961 cggggccggc gaaaggaatg gagttgggag cggcgggagc gaggtcgacg tacgaggggc
    19021 gattattgac aacgaccggg acattccggc cgaccttgat cttgaagcct tcgatttcca
    19081 gctcgaattc ggacagattt ttttcctcga gaagtttgat gattttattg atttcttcaa
    19141 aatctatttt cggagccata tcctcctcct gaaaaaaata agtccatctt ctttattttc
    19201 ttacggttaa aattatcccg aaatattctg gaaaaaatcc tcattacgtt accctacgaa
    19261 ccggggactg tcaagcaatg tgaatcgaca agatatatcg ctttattgtt ttttctcgaa
    19321 gaccgaggcc ctccggccgg cgcaccagac atccttgacc tggaacttgt aatcgagcag
    19381 gacgatatcg gcatcggccc cggggagaag cgttcccttg cgcttctcca agcccaaaat
    19441 ccgggcggga ttggaggtcg ccagaggaag aacatcctga agcgaccagc cgctccattc
    19501 cataagtttc aggagcgcct cgaggagacg gaggttgctg ccgaaaagcc cccccgcggc
    19561 cgtcaaaaga ggcgcttcct tcgttcccgc gttccggctg gcatcggtga cgaaaacgac
    19621 tttatccgcc ggcttcgcct ggagaaccag gaaaaccgcc gccgggtgaa gatgacgtcc
    19681 gtcggcgata agctccacgg aggcccgctc gttcaggagc agagctccgg ccagccccgg
    19741 ctcccggtgg aagaagccgc gcatggcgtt gaagaggtgc gtcccgtggc tgatcccggc
    19801 gtcgaagccg cggatcgctt cctcgaacga agcgtcggaa tgcccggccg aggcgatgat
    19861 cccccggcgg acaagaaact tgatgaacgc gggggcgccg ggaagctcgg gggccagggt
    19921 catgagacga atgggcgtgc gaaaaatccg gaggtactcc cggatttccg cctcgtcgat
    19981 tcttcggacg gctccgggag gctgggcccc gtgccgcttc ttggaaataa acggtccctc
    20041 caagtggatc cccaggatgt ccgggccggc ttccgtcgtc gccggcaggg aaccccgttt
    20101 cccccgcggc cgaagggcgt cgaccgcggc cagggctttt ttcaaggccg ggcgggaggc
    20161 cgtcatcatc gtcggcagga ccgacgtcgt cccgccccgg acgtgggtct cgaggaccgt
    20221 ccgcgcggcc tcgaccgtcc cgtcaatgta gtcggcgccg ccgccgccgt gctggtggat
    20281 gtcgatgagt cccggaacgg cgatgagatc gggggcctcg acgacgtcca tcgtgaaccg
    20341 gtccgattga tcgtgggcct tgcggacggc cgcggcgatt tccttgtccc ggccgacggc
    20401 gacgatccgg cggcccttga tcaggacggc gccccggcgg atttccttgt cgggcgtcag
    20461 gacgacgccg gcccggatta aaatataggg aaccgggggg cggccccttt tcacggcttt
    20521 cgttttcatt ttacgggcag gtttggttgt ttgcggcatc agatttttct ccaagcggcg
    20581 gcgatttccc cgcgcagcgc ggccagggcc cggcgggccc ggatttccgt tttcgcctcg
    20641 gccagcaccc ggatgaccgg ctcggtgttc gagggacgga ggtggatcca atgatccggg
    20701 aaatcgactt tcaatccatc gagcagattg accgttccct tgtcttcgta tcgttttctc
    20761 aacacccgaa tcaggcgatg ggcgcgctcg gccgatccct cgatccgatc cttgaggagc
    20821 acatagcggg gcagggctcc ggccagggcc gaaaccggct tgtggccggc cgccagggcg
    20881 tccagaacat ggcccatcgc cgcgaagctg tctcggcacg gatggatttc cggatcgatg
    20941 actcctccat tcccctcgcc gccgatcacg gcccgcggcc tggcagcgag gaccttttcg
    21001 acgacgttga cctcgccgat ccgggtccgg aagatcttca ctccgtgccg ggcggccaag
    21061 tcgtccaagg cccgggtcga ggacatgttg cagacgatcg agcctttcgt tttttccagg
    21121 acgcggctcg ccgcccagac gagggtcatc tcctcgccca gcgggcggcc gcgctcgtcg
    21181 atgatcgcca gccggtcggc gtcggcgtcc tggacaaagc cgatgtcggc ccgggccgtt
    21241 ttcaccgccc ggcagacgcc ggcgatgttt tcgggggtcg gctcggaagg atggacgaat
    21301 tccccggtgg ggtcgcacgc cagcggaacg acgtcgcagc ccagctcccg aagaaaacgc
    21361 ggccccagga cggctcccgc gccgttgttg cagtccagga cgacgcggat ccggcggcgg
    21421 cggatcgcct ccccgtcgaa ccggttgagg agccggcgga gatgggcgtc ggcggcgcgg
    21481 ggcaagacct caatggtcct gaatttcccg gcggcgacga aatcgaaggc gccctggtga
    21541 tagatgtcga ggtattcctc cgtctgctga ggcgtgaggt acagtccgcg gcggctgatg
    21601 aacttgaggc cgttccattc ccggggattg tggctggcgg tcacggcgat tcctccggcc
    21661 gcccggacct ctttcgtcaa gtacaggaaa gaagggatcg ggaggatgcc gacgtccacc
    21721 ggccggcagc ccacggccag gagaccggac acaacggctt cggccagcat gatcccggag
    21781 gagcgggcgt cgcggccgac gagcaccggc ccgccgccga cgtaggttcc gaacgcctgg
    21841 gccagggccg ccgccagctg gggcgtgaga ctgtcgccga tgattccccg cgctccggag
    21901 atgctgattt tcagagtcgt cgcgcttttc atgggatcat ccgctccagg gaccggagga
    21961 tctcgtcgac tttctccctg gcccgctccc gggaccggtc ctcggcgacg acgcgaagca
    22021 tcggctcggt gccggacgcc cgcacttgga cccagccttg cttgtcttcc acccggatgc
    22081 cgtcccccgt gtcgacgtcg cggccgcgga acatccgggc gatttcgctg atgacgccgt
    22141 ggatgcggga cgccggacag tagaccttct ccttgacgat gtgataccgg gggaggcggt
    22201 cgacgagggc cgacagccgt tggccggtct tggccatcgt ttccaggacg aggcccatcg
    22261 ttaagaaggc gtcgaaggcc ggctggaagg cgggaacggc gacaccgccg ctaccctcgc
    22321 cggcaaggat cccgtcctcc tgggccaagg ccaggaaaat cggagcgggc cccacccggg
    22381 tccggatcag cgggcatcgg cgggcccggg cgacatcctc gatcatccgg gaggtggaat
    22441 tattggtcac gaccggaccg gagcaccggg ccaggacgga atcggcgacc aggggaaacg
    22501 tgtattcctc ggaaagggct tcggctgttt cgctgaccag ggagacgcgc ccggcgtcgc
    22561 tgttgagaag aaagcccgcc tcagccccgg ccgcccggac gaccgaggcg cactgagcgg
    22621 cgttccgcgg ccgcggctcc ggatcgtggg ggaagaatcc gttaggctcg tcgttgagcg
    22681 ggatcaagtc gaaccccagc gtgtcggcga accgggctag aaacggcgcg ccggctccgg
    22741 cgcaggcgtc gatgaccacc cggaagcggg ccgcccggac ggcggccgct tcgatgaaag
    22801 cgccgagctc cttgaaatag aaatcggccg tctcggccgt cgggaccgga atgagccggc
    22861 cgagatggtc cacggccgct cgctcgaatc tgccgagatg gtaataatcg aggacttcct
    22921 ccccttgaaa gggattgaga ggggagccgt ccgggccgat gaaagccaag gcattccagg
    22981 aaacgtcatt gtggccggcc gtgatggaga cgcccccgcc ggccttgcgc ctccggactg
    23041 ctttttgcag caggggggta ggacagacgc cgaggtccag aacgtcgcat ccggaggaga
    23101 gaagggcgga aacgcaggcc gctcggagca tggggctgtg aatccgcgtg tctcggccga
    23161 cgacgaccgg cttgcggtcg atgaaagccc cgaaggccga ggcaaaatcc atgaccatcg
    23221 tcggcggcat ggacgggccg acgactccgc gaaccccgga gaggccgatt ttcagcatga
    23281 gctccctaat tataacaaaa gaaaagggcc gccgcacgaa ttcggagcgg attttcgaca
    23341 ttttccccct gaaaacgcct tcctttgctt ttgcccgcct cttcccctat catgagaatc
    23401 ccatgtttga tcagttgtcc ggccgtcttc aaaaggcgat gaaatacctc cgcggggagg
    23461 cgaaggtgac tgagggcaat atgtccgagg ccctcaggca gatccggctt gcttttctgg
    23521 aagccgacgt caattacaaa gtcgtcaagg atttcgaagc ccggattaag gtccgggccc
    23581 tggatcagac cgtcctggac agcctgactc cggcccagca agtcatcaag atcgtccggg
    23641 acgagctcgc ctccgtcctg ggaacaacgg acaagcccct gacgttcgcc tccgcaccgc
    23701 cctcgatctt catgctcgtc ggcctccagg gaagcggcaa gacgacgacc tgcggcaagc
    23761 tggccaaatg ggcggccggc aagggccgca gtcccctcct cgtttcgttc gacctcaagc
    23821 gagccgcggc ccaggaccag ctccggacca tctccggcca gctccatctg ccgttttacg
    23881 agatgacggc tgcggccatg gccaagcccc ggtcggccct ggccgacctt cttcaatata
    23941 cccgcaaccg cggatacgat gtcttgcttg tcgacacggc cgggcggctt cacgtcgacg
    24001 acgaattgat ggacgagctt cggatcgtca aggatgtcct ttcgcccgtt gaaacgatct
    24061 atgtcgggga cgccatgacc gggcaggacg cggttaagag cgccaagacc ttcgccgacc
    24121 ggatcggcct gacctcgatc attttgacca agctggacgg agacgcccgg ggcggggcgg
    24181 ctctttcgat cgtcgcggtc acgggaattc ccatcaagtt catcggcgtc ggcgagaaat
    24241 tcgacaagct cgaggtcttc catcccgacc ggatggcttc ccgcatcctg ggcatggggg
    24301 atatcctgag cttgatcgaa aaggccgagc aagaaaccga cctcaaggaa gccgaagccc
    24361 tggccaagcg tctccggcgg caggagttca ccttcgatga ttttcggtcc caactccgtc
    24421 agttgaggaa aatgggttcc ctcgaggggg tgctcggcca cctgccccag gccgggccgt
    24481 tcaagaacct ggccggggcc cggatggacg aggcccggct gcggcatttc gaagccatca
    24541 tcgattcgat gacggccgcg gagcggaccg atccgaggat gatcaacggc agccgccggg
    24601 ctcgcatcgc ccggggcagc ggccggccgg tgagtgaagt gaaccagctt ctccgccagt
    24661 tccaagagat gcgctcgatg atgaaaaaat ccatcttcca gaagatgatg tcgtcgttga
    24721 aatgaacggg cggccggttt ccggtcccgc ccgggagcgc tgatcgcaag gaggaatccc
    24781 atgtatgtcg tcggcgtgac gatttggatc aaaccgggat tcgaggaaaa gttcatcgaa
    24841 gccgtccgga tgaatcacga aaagacgcgg cgggaacccg gaaacctgcg cttcgacgtc
    24901 ctccggcatg aggacgagcc cggccgtttt tttctctatg aggtctaccg gagccgggac
    24961 gatttcgccg cccatcagca aaccgcccat tacctggctt ggaaggaaac cgtggccgac
    25021 tggatgtccc ggccgcggga agggatcaag cacttgagtc tctttcccga ggatcgaccc
    25081 gccgcctggt gagatcccgc catggttttt gaatggaccg cccccgcccg gatcgtcttc
    25141 ggccgaggcg cggcggccga tatcggacgc cgggccgcgg agatcggcag gcgggttctg
    25201 tttatccgga ctggagccct tccggcgaca ggcgggacgg tcgaggccgc gcgaagcgcg
    25261 ctggcggccg aagggctgga gtttctcgaa gccgaggtca ggggggagcc ggaggtcgaa
    25321 gccgtcgacg ccttgaccgc ccgggttcgg ccgttcgaac cggaggtcat cctcggcttg
    25381 gggggcggca gcgcgatcga tacggccaaa gccgtggccg ggttgttggc aggcggcggg
    25441 gaagtccttg attatctcga ggtgatcggc cgaggccggc cgttcagccg ccccggcgtt
    25501 ccggtgatcg ccgtgccgac gacggcgggc acgggggccg aggccacgcg caacgccgtc
    25561 ctggccagcc gggcgttggg ggtcaaggcc agcttgcggg ggccggccct cgttccccgc
    25621 ttggccttgg tggatcccgc cttgaccgat acccttcccc cggccgtgac ggcttccacg
    25681 gggcttgatg ccctggccca atgcctcgaa tcctacctgg ccctgaacgc ccggccgtgg
    25741 acggacgccc tggcccttga aggcttgaac cgggccgctc ggtcgctcgt tcccgccttc
    25801 cgagacggga gcgtccttga agcccgcgac gacatggccg tggcggccct cttgagcggg
    25861 atctgcctgg ccaacgcggg cctaggagcc gttcatggac tggccgcggc cctgggcgga
    25921 aaatttccca tcccccatgg agtcgcctgc gggcgggttc ttccaggcgt gctctcggaa
    25981 aacatccggg ccatgatcgc cgggggcgcg ggccggacgg ttccggccaa atgcctccgg
    26041 ctggcggagg tcttcctccc cgcgagacgg ggatccgaaa aaacacggat ggccgccctc
    26101 ggcgaccggc tttccgcctt cgtcgaggag cttgaacttc ctcgcctttc agccttcggc
    26161 gtgaccgagg ccgacctgcc cgtcctggcc gaagccgcgg ggcgctccag ctccatccgg
    26221 caaaatccgg tcgcccttga cgaaaaaacg ctgatcggca tcctgcgggc cgccctgtga
    26281 tctccggact tgccgcgccg ggaatgacgc gctgtttcgg cgctggtttt cgggtcgacg
    26341 ggaaaagaat aaaaggagcg gatcggttgg ttgactttca agcgggtttt ggctattatc
    26401 tacaaaccat tttaaaagaa cggattacac aattatgttg tcactgcgtc ttatgagatt
    26461 tggagccaag ggccagcccg cttaccgggt cgtcgtcatc gactcgaaaa agccccggga
    26521 aagcaaggcg aaggcgatca tcggatccta taatcccctg accgatcccg ccgaaataaa
    26581 aatcgacctc gccaaagcaa aatactggat ggaccgggga gctaaaccct cggaaaccgt
    26641 caaatcgctc ctggccaaac cgcagaaagc gactcccaaa gcctgatcgc cggggcgatt
    26701 ttcccgaagg aggagaacgt gaaggaactc gttgaattga tcgcccaggc tcttgtcgac
    26761 cagccggaca aagtcgtcgt ttcgcagatc gagggcgaac aaaccacgat tctggagctc
    26821 aaggtcgctc ccgaggattt gggcaaggtc atcggcaagc agggccgcac ggcgcgggcc
    26881 atccgcatca tcctcggagc cgccgggatg aagctgaagc accgctacaa cctggaaatc
    26941 atcgaaaaat aataccgtga aacgcctggg ccggattctc cgaacccagg gccgccgggg
    27001 ggaagtgaag gcggtcctgg acgccgctcc ccgcccgggt ggtttttttt tagaatcttt
    27061 gtcagggaaa acgatgaatt ccgcgaatac gcggtcgaat ccctgacgcc ttataaagac
    27121 gcccatacgc tgaagctccg gggcgtcgac tcgattcccg acgctttgcc cctggccggc
    27181 cgggaaatct tcgcccttca tgaggacgtc gcccctccgg ccgaggatga attcgccgtt
    27241 gaagacttgg ccgcgtgccg ggtcgtgacg aaagaaggcc gtcccgtcgg ccttgtccgg
    27301 ggagtctggg acacgggtgg aacaccgttg ctcgtcctgg atccggacgg cggggggaat
    27361 gagatttaca tcccgcttag ccggagcatc tgcccggtca tcgacatcga agccaagcgg
    27421 atcgtcatcg atcctcctga cggccttttg gacttaaatg agatttgata taatcacgat
    27481 cttccccgag atgttcccga gcgtctttgc ggggggcgtc atcggcaagg ccgtccaaag
    27541 gggacggttg gacctgcggg tgcataacct tcgcgatttc acgacggaca agcaccagca
    27601 ggtggatgac cgcccgttcg gcggcgaaga aggcatggtc ttcaagcccg agccggtctt
    27661 cgccgcggtc gaagcggtcc ggggttcgga ggatccgccc gtttatctcc tctcgcccgc
    27721 gggccggagg ttcgacgacc ggctggccag ggaaatcgcc ctccaacctc gggtcgttct
    27781 gatttgcggc cgctacgagg gcgtggacga gcgggtcgcc ctccatttgg ccacggacga
    27841 gatctcgatc ggcgattatg tggtcacggg aggggaattg ccggccatga tcatcgtcga
    27901 cgccgtcgcc cggcacattc cgggcgtcgt ggggaaggaa ggctcggtcc gcaaggaatc
    27961 gttcgccgag ggcttgctgg actatcctca gtatacccgc ccccgggatt ttcgggggat
    28021 gaaggcgccg gaggtgctgt tctccggcga ccatgataaa atcgaagcct ggcggcgccg
    28081 caaggcgctg gagaagacgc ggcggagaag gcccgacctt ttcgcccggc tgtcgctctc
    28141 gcccgaggac cgcatgttgc tcgacgaaat cgacaacgaa aggaacgaac aatgagcctg
    28201 gtacaagtga cggaagacaa ggtgatgaaa aaagatctcc cgcctttcgc tatcggcgac
    28261 acggtcaagg tccacgtcct catcaaggaa ggcgaaaagg aacgcgtcca aattttccag
    28321 ggcgacgtga ttgccaagaa aggccgcggg atgagcgaga cgttcaccgt ccggaaggtt
    28381 tctttcggca tcggggtgga acggattttt ccgcggcagt cgccctggat caagaaagtc
    28441 gaggtcgtcc gcagcggacg ggtccggcgg gcgaagcttt attacctccg cggactgaaa
    28501 ggcaaagcgg ccaagctccg cgaagaacgc tgatccgcgc cttgcccgat ttcgagattg
    28561 aacgccggca tctcgaagcg gggaggacgc tgatcgccgg cgtggatgaa gccggccgcg
    28621 gggctttgtg cggtcccgtt gtggccgcgg ccgtcatcct tccgttctct tggtttgccg
    28681 gcctgccgcc cgattgggtc gaacggctgg atgattccaa gcgcctttcc cccacgcgac
    28741 gcgagcggct cgcccggcgg atccgccaag aggcctccgt cggtctcggc ctggccggaa
    28801 acgccgagat cgatgtcttg aacattctcc gggcgtctca ggaggcgatg cgccgggccg
    28861 tggccgcgct gcccgttccg cccgacatgg tcctggtgga cggcctcgcg atccgggatt
    28921 tcccccggcc tcaggacgcc gtcgtcggcg gggaccggcg gagtttctcg atcgccgcgg
    28981 cttccatcgt ggccaaggtt tcccgggacc ggatcatgat ccgggcggcc cgccgttttc
    29041 ccggctacgg gatggagcgg cacaagggct acggcacgcg cgctcatttt gaagccttgg
    29101 ctctccgcgg tccgacccct tttcaccgga tgtcgtttaa tctacaatac caaccgaagc
    29161 tcttctgaat ccatgactat taaagcttcc gaccgcgcca aaatctccga acgggcgggc
    29221 caatttgtca aggcgggacg aatcgaggaa gcggtcgccg aatatcaaaa gctcctggac
    29281 ggatccggcc aggatgtctc gatcggaaat atcatcggag acttgtgcct ccagctggga
    29341 caggaagacc gggccatcga gcttttcatg aataacgtcg aaacgctgga acggcggggg
    29401 gcgtattcgc aggccctggc catcgccaag aaggtcaaca agatccggcc ggcggaaccg
    29461 gacgtcatga tccggctcgg ggatctctac gggcagctcg gattttcggg cgaggcccgg
    29521 gaacaatata ctcaagcggc ggatgagtat gaaaagcgga atgagaccaa gccgctgatc
    29581 gccctctacg aaaagagggc ccggctcgac cggaccgatc tggtgacccg gctgaagctc
    29641 gcccgcctct atctggagga gcggaaaggg gatcaggccc aggtcgtttt gaacgaggcg
    29701 gccgatctgc ttttcgtccg caaggaatac ggcgaggccg aaaaaatcct cctcgaggcg
    29761 ctggccgtcg ccgacggcga ttaccggacg ttgacgaacc tcgttcgcgt ttaccgcgag
    29821 ctcaagcggc ccgagacatc catggaaatg ctgggccgga tcgtcaagaa gcgagggccc
    29881 cggaccgacc ttctcagcct gatggctctc ctgcacagcg agcaggggga caaggacaag
    29941 gcggccgaaa tctacggccg gatcctggcc gaggatgccg ggaactacga catccgggcg
    30001 aaactcggaa tcattgagat cgagcgcgac cggccggatg aggctctgga gatctttgat
    30061 ccgcttatat cccattttct ccagaaaagc gacgaggata aagccgccgg cctgctcggg
    30121 ctcattctga tgtccggccg gatgcatctt ccgacgctcg agcggctggc cggcgtcttc
    30181 cgcggcgtcg gccggacgaa ggacctggaa gtcgtgcttc gggtccttct ggcggaatac
    30241 cgttccgaga gtcgggacaa tgaccggatc cggattctcc gcgagctcta tatcctcctt
    30301 ccgatggatg ccgggatcgc cgaagaagcg gagactctag ggatccggcg cgagctccat
    30361 caggaggccg ccgaggtggc gcccgaggcc ggccggccga tttcggaaca ggaccaggaa
    30421 atgctccggc tgaatttggc caaggccgag ctttacctgg aacaaggcct cgtccggaac
    30481 gcccggcgga tcctggagaa cctgcggctt ctctaccccg acgatccgcg catccagaag
    30541 aaaatcgaaa gtcttcgggc cgccccgagc gttatcatcg acaagaaaat ccctgagatc
    30601 gtcgaggaaa cggcccgccg ggagatcgac atcatcggcc ccgcggttga accgggaatg
    30661 gttgtccctc cggtttcggc tccgcccctc gacctcgaat tgctcatttc cgatttcgcc
    30721 cagcccttgc ctcccgaaac cccgattcat ccggccgccg aaacgccgtc gagccggaaa
    30781 aagggaccgg ttttttccgt tcggagcggt ccggccgcct cccggacgaa acccgcgcct
    30841 cctccgccgc cccccgcgcc tcaggccgag gcgcccgacg aagaggccgg atccaaggtc
    30901 acgtccgccg aaatattcgc cggcttggat atcggcccgt tcgtcgcttc ggcagccggt
    30961 tccgccgccg cgtcccaagg gtatatcgac atcaccgaca agatcgagga ggaaatcgag
    31021 gctctcgaag tcgagttcta caagcagctt aaggagcgga cctcggtcat cgagaaggac
    31081 ctggtcgata tcgtccagga attccgccgg caggtggata tcaaggtgga tcagcgcaat
    31141 tatgaatccc gctacaatct gggcttggcc tttttcgagc agaacctcta tgaggaagcc
    31201 atcgccgagt tcgaattggc ggccgccgat ccgggccgga cggcggattg ctggagtctg
    31261 atcggacaat gctgcgtcaa aaaaagaaac tatccggaag cccgccgtcg tttcgagggg
    31321 gcgatttcct tgacgccgga ggaatctccg gagcggttcg ccttggcgta cgaactggcc
    31381 tcgttttacg agatgatcag cgaaaacgag gcggccctgg ctctttaccg cgagatttcg
    31441 gcctggaatc cgaagttccg caacacggcc aagcgcatca agatcctcga aaaaatcgtg
    31501 tcctgaccgc ttcctctttc actgtgcgca tcatcgattt aagccatctc ctcgccgaaa
    31561 cgatgccggt ctttcccggc gacgcggccc cgcggatcac ggaagcctcg accatcgatg
    31621 aacacggatt cgcctcgaaa accctccgcc tggggactca caccggaacg cacgtggatg
    31681 cgccggccca ttttctggcc ggcggcgcgg ccctcgaccg gctgccggcc gcccgcttcg
    31741 ccgggcccgg ctgtctcgtg gacgtctccc gccttgccgg gccgcggatc gagatcgccg
    31801 acctggaggg ggaccgggac cggatcgcgg cctcggaatt cgttcttctc ttctccgact
    31861 gggcgaagcg ctgggggagt cccgcgtatt tctccggcca tccggcgctt tcggagaagg
    31921 ccgcccgctg gctggccggc ttcaagctga agggagtcgg ggtggacgcg ccttccgccg
    31981 acgtcatgga tgcggcccgt ccggccgtgc accggatttt tctgtcccgg ggtatcgtga
    32041 tcatcgaaaa cctgaccggt ctccggacgc tccgcggccg ggcgttcctc ttcgcctgcc
    32101 tgcccctgaa catcgccggc ggggacggct cgcccgttcg ggcggtggcg atcctctgat
    32161 cccggccttc aggatacacg gttcgagcga acggttcgcg cggggacctc ttcgccgggg
    32221 agcaaacacg atccttcggc acgcccgacg ggctgtcctc ctcatgaaga cgatgttgag
    32281 gacaagagat agacaggaat ccgaaaagat cttatgaatg ccgccggccg aaaaagggat
    32341 gggaaaaaga gaaagcttct cccggtcaat tcattttatc cagcgggacg tccaggacgg
    32401 gtttgtcctc gaagccgcgg aaatcgaaat gccaccattc cggggcgtag ggaatgaatc
    32461 cccggtttgt catggctagt tcgagaaggg cgcggtgggc gatggcttcc cgcggcaggt
    32521 ccatgaagtc gcgggccgcc cgctcggtga aatcatcgaa cggcgtcggc atgaggacct
    32581 cgcggccggc ggcgtcgacg agggtgagat cgacggcgta gccgcggttg tggcgcgatc
    32641 ccttggccgg atcggccacg aaccgctcgt cggggagaat cgaccagaag attttttgga
    32701 ccgaaagggg acggtagccg tcgaaaacct tgaggccgta gccgaaggtt cgcagatccg
    32761 cttgaacttg gaccagggct tgagcggctt ccttgacgag gaagcaacgg gcgatgggat
    32821 agacggcctt gccggtgaag ttgtcggtcg tcgcgtactt gatgtccagg atgatgtccg
    32881 gggcggcgac ggcaagctcg accagctccg gcggggcggc cgcgaagtcc ggggcggcgc
    32941 cggggcggca cccgagggaa aggaaagctc cggccaggag tactacgacg gcggcggcgg
    33001 cgaggggtcg cggcccgcga tcggcatccg aggcggcgcg cctcccggga tgaatcggaa
    33061 tcatcctgcc gcgatcgagg ctggttgtgc tcatgaaaac gctccttcgg atttttccct
    33121 agagaatcat ttttgtcccc ggaaatcaag gttcaaatga aagaatcctc tctcccgggc
    33181 cgatccccaa agggaaaagg cgccggagga accccgggcg gcgggattcc ctccggcgcc
    33241 ttttcgccgg gccggggctt atttaaccgc tttcccgtaa aggatcagga tcagtccccg
    33301 gtcggcgtcc gtcttggaaa cgccgacgac ggtcttttcc ccggccttca ggagaagggt
    33361 cgtgcggatg agctcgttga agcgcgaggc cgccggcggg gcctcctctt ttttcaccag
    33421 gccgaccgtc accgccgggt tccattcctg cctgcccagg cggatctcgg tctggatggt
    33481 ttcgaccgtt ccgtccttcg tgtatttcgg tctcagccag acttcgtatt cccccttggg
    33541 tccgattttc gcttgagccg tctcgccatc gatcacccgg atgaccgtcc cgtcgaggag
    33601 gacgaagctt ttatatttca ggatgccgcg aagctcccgg atgaccgcat cgttcttcag
    33661 ggtatcttcg cccgcgtccg atccctggac gatctggact gtgtactgga tctcggccgg
    33721 cttgatgtcg atgtcccgaa cgatggatat cattttctcg acgatctccg gcgtgtcggt
    33781 gacgacgatc gatttctccg cgcgcccggc cggcatgacg cggccgaggg cgctttggta
    33841 ccctcgaagg agaatcgtga tcgtttcgag atcggcgtat ttcagcttga tgagctcggt
    33901 ccgaagggcc ggggcgggcg gcgcggcggc ctggggcgcc gcttcaggcg gctcggcggg
    33961 ggaggccggc atggaagcgg acgcggccgc cgggttttgg ctctgggccc ggacggcggc
    34021 ggcgaaaacg aacgtgagcc cggcgagaag aaagattcca acggattttt tcatgtcatt
    34081 cctccaggtt gagagagtca ttgataaacc agacgatctg aagaccggtc tcttccgata
    34141 tccacgtgaa agacgggacg tctcccgccg gcgggggcgc tgcggacgaa ggaaaggggg
    34201 acgggcggcc ggcggaaaag acggttcccc cggccggggc ccgggagtcc gtttccgccg
    34261 gaatggtccc gggcgcgttc ggcttgatga tccgaggccg cgcggcggag gccgcggccc
    34321 gggagggatg gttttccacc ttggggatga gccagggaat atgccggacg ccgaaaaata
    34381 tggcggcgcc gacaagaagg acgcccagaa cgcagccgag ggaggggcgg aagaaccggg
    34441 cgaaacggtc cgatttcgcc gtctttccgc cggcgacggc cgcccggatc gcgaccctcc
    34501 attccgcctc ggtgaaatcc gcgtcgtcga aaacggccgg atgcttcaag gcctcgatcc
    34561 cggcccgaaa ggcggacgcc tcggttcggc aggaggcgca ttcccggagg tgctgttcca
    34621 agcgcttttt cgatccggcg gagaaaatcc gtccggtcgg atcggaaagc cgtctgcggg
    34681 cggaccggca attcatcggg aggtctcctg aagcgaagga tcgagggcgg ccaatcgatc
    34741 gcggatttta ccccgggccc gggagagatg gacgcggacg gcgatcgccg agctccggac
    34801 gatcgcggcc gtttcccgga cgctttttcc ctccaggtcg cggaggatga atacgtcccg
    34861 ctcccgggcg ctcaaggcgg acagggccga gaggactcgt tcccggattt cttgccggcc
    34921 gcgggcttcc tcgggccccg gtccgccgcc gccggcgttc tccagaagga aatcggagtt
    34981 cgcccaagcc gtttcccggc gtcgccggcc ggctcgctcg cgtccgacgt tgaccaggat
    35041 ctggagcagc cagttccgga agccgcgttc gaaatcgcat ttcccgagat ggcggaaggc
    35101 ccggaggagc gtttcctggg cgacctccaa ggcatcttcg cggttgccgg tcatcttata
    35161 ggccagtccg aacagcgtct tccggtaggg aagaacgagc ggctcgaacg ccgtggaatc
    35221 tccggcgagc acccgggcgg ccaattcgat ctccttcacg tccatttcac aaccgagtat
    35281 atcgccggag gtccggaaag gttaagggcg gacactccct cgatttcccc ccctggctgg
    35341 attctccttt cctctcaata agaaacggag gcgcggatcg aaggaggatt tataaaatcg
    35401 gggcggcggc ccaaattcag cggcggtcgg ccggcgtcac ttgaccgctt tcatcgccgc
    35461 cagggcccgg gcggccatcc ggtcctgaaa atcggccttc tcggccggct cgggccgggt
    35521 tttcaggaac gcctcggttt ccctcaggat ggcctcctcc gcctccagca gcttcggcag
    35581 gaagcccgtg ccgtcggcgt tgtcgagccg ggagaccagc agatattcgt ttttatcgta
    35641 ctcgtcgatg ggatggatga gggtcttgat ccgggcggat tcctcccaaa gaggcgcgtt
    35701 ctctcccttc caaaggggct ccgggctccg gccggcctcg acccaaagag gaacggccac
    35761 ggccgtgagg ggttcacccg ggatgaccca catcgtcgcc gaggattccg gatcttccgg
    35821 atttttcccg acgatcacca cggccgctga tgtgtcgggg cggttgatgg tgtggcgggc
    35881 gtgaatccat aagccgggct cggccgggat atttttccac tcttcgggat cgggattccg
    35941 cagcagcgcg tggccgcagt cccggctgaa gcgcgtcagg atcgtttgaa aatcgatcgg
    36001 ccggccgggc ggcagggagg cgaacaaccg gccggcccgg tcgaatcgaa ggtagccggc
    36061 ccccgccccg gcccggccgc tgcgggcgaa gttggtgacc accaggtatt tttccggggc
    36121 cgcggacgcc tcgaacttct caaagccgtg gttgtgaacc tcgaagaggc aggctcggcc
    36181 gcttccgtcg atgaccccga agttggcccg ggcgccgagg tcgggaccga gattggcccg
    36241 gaggagccgc tcgaagtcct cgaccgtggc gcacgtgcgg agggcgtcgg ccatgatgac
    36301 gccctcgagg tcctggagct ccccggattt ttggggcagg ttataagcca cggtgttcat
    36361 gacggcgaaa ccggcggagt tgaggccggc ccaggcttgg cggcccgagg cgccctcggc
    36421 attgatcagg gccgcgtagc tgtagggcgt ctcccggacg aagacgacct tgttggacag
    36481 gacggcggtg tcgcggtttt tccacagcac cgctccgccg gcggcggaag ccgccgggct
    36541 gatcacggcg gccgtgcatt gctcggcccg gttctccccg aagacggccg gggcggccag
    36601 taaaaaagat cctgcgatca agagccgaag tccacggtca aagcgcatgt tgttttcctt
    36661 aattgaaggg gggaccaggc gccggtttgg gggagacgct cgttctcccg ccccggcccg
    36721 ctccctccct cgaggcatgg tatcctcggg cgcaaacggt gtcaagaaac ggaggcctgg
    36781 ttttccgatg aacaggaaaa gcagcccgcc gcggcaaagg gaccgtgacg gctcggccgc
    36841 cggagcggcg cctccatgac gagaagcgcg gagaatgatt cggcttaaat gcggcgcccg
    36901 atttgtcaaa gagcctccag cggcttgaca ggggagggag gcggacgtta taagggtttt
    36961 atcccgccga tgaggagcgc ttatgaatac ggaatccctg ttgatcgcca aaggaaccga
    37021 ggagatccgc cttctgcccc gcatggccaa ccgtcatggg ctgatcaccg gggcgacggg
    37081 cacgggcaag accgtttcgt tgcaggtgct ggcggaaaac ttcagccgga tcggcgtgcc
    37141 ggtcttcatg gccgacgtca agggcgacct cagcggcgtc agccggccgg ggacggaaac
    37201 gcctaagatc ggcgagcggc tggacaagct cggcctgcgg ccgtttgaat tcgcggggaa
    37261 gccggttgtc ttctgggaca tcttcggcga ccagggccac ccggtccgga ccacgatcag
    37321 cgacatgggc ccgcttcttc tcagccggct cttcaacctg agcgagactc agagcggcgt
    37381 cctgtctctt gttttcaagg tcgccgatga caacggcctt ctcctcctcg attccaagga
    37441 tctccgggcg atgctccagt actgcggcga ccacgccgat gacttccaga ccgagtacgg
    37501 ccggatctcg ccggcttcga tcggggcgat ccagcggtcc cttctcgacc ttgagacgca
    37561 gggcggcgac cggctgttcg gcgaaccggc cctcaacctc gccgatttca tccaaaccga
    37621 ttcccagggg cggggcgtga tcaacattct ggcggccgac cgtctcatgc tccagccgaa
    37681 aacctacgcc acgttcctcc tgtggatgct ggccgagctt tatgaaaaca tgcccgaggt
    37741 cggcgacctg gagaagccga agctggtctt tttcttcgac gaagcccacc tcctgttcac
    37801 cgacgcccct gccgtcctcc tccagaaaat cgagcagatc gtccgtctga tccgctcgaa
    37861 gggagtcggc gtttatttcg tcacccagaa tcccttggac gtgcccgaca tcgccctcgg
    37921 ccagcttgga aaccggatcc agcacgccct ccgggcgttc actccccgcg atcagaaggc
    37981 ggtccgagcc gcggccacga cctttcgggc caatcccaag ctggatgtgg aaaaaaccat
    38041 caccgaactg ggggtcggcg aagcgttagt ctcgttcctg gacgagaaag gccggcccgg
    38101 cattgttgag cgggcgttca tcctgcctcc ccggagccag atcgggccga tttcggcgga
    38161 cgaaaggcgc cggatcatcg gaagctcggt gttgtacggc cattacgaac ggcagatcga
    38221 ccgggagtcg gcttacgagc atctgaagga aaaagcccgc caagcggagg aggcggccgc
    38281 ccgggccgcc cgctccccca ttccgaccgg ttatccccaa ggaccctcct atgagccgcc
    38341 ccgggccccg cgtccggtcg gccggccgag ggacaccttt gttgaatccg tcggcaagag
    38401 cgtggcccgg tccctggcca cctcggccgg ccgccagatc atccggggcc tcctcggctc
    38461 gatcttgggc ggccagcgcc ggtaaccgcc ggccggcggt ctctgttccg cgttatttac
    38521 gaccagcgcg gtgagaaaaa accgcggtcc gctccgttat ttgaatgtca acggaacgga
    38581 tcatgagcga agtcaacgac ggcggaaacg ggggagccgg gcgggacatc gtcctcctcg
    38641 ggcggagcgc gtctttcgcc cgcgttcgcg agctgatcga ccggctgaaa gacctggaca
    38701 ctccggtgtt catctcgggg gaaagcggca cggggaagga agtcatcgcc caagcgatcc
    38761 atttccgcgg cgcccgggcc ggcggccgat tcgtcgccgt caacggcggc gcgatccccg
    38821 ataatctcct cgaaagcgag ctcttcggct atgccaaggg cgcctttacc ggcgcgctcc
    38881 gggacaagcc cggcttgatc gaggaagccg gagggggaac gttctttctc gatgaggtcg
    38941 gcgacttggg attcccgctc caggcgaaac tactccgggt tcttcaggag cgcgagatcc
    39001 ggcggatcgg ggagacgcgg acccggccgg tcgatgtccg gttcatcagc gcgaccaaca
    39061 aggatttgga gcgggaagtg agcagcggcc ggttccgcga ggacctttac taccgtctcc
    39121 ggatcgtaac catcgacgtc ccgccgctcc gcgagcggcc cgacgacctg gagctgctcc
    39181 tccaccattt tatcgcccgg tacggcgaaa agatcggccg gcctcgggtt tatttcactc
    39241 cccgggtcat ggaaacgctg ctggcgtacg cgtggcccgg caatgtccgg gagctccaga
    39301 acgaagttca gcgctgcctg atcctgtgcg gcaacgaggg gatcgtgcgg gtcgaacacc
    39361 tgtcgccgcg tctcaatccc ggccggtcgc cgggcccggc ccccgaaagc ttcaacttct
    39421 ttgaggccaa ggccgatttt gtccggcggt ttctcggcca ggctctccgc cggcatgaaa
    39481 acaacaaggc ccggacggcc gaggaaatcg gcctctcccg tcaggctctc tttaagctac
    39541 taaaaaagca ccggattacg gcgcccggcg ccgccgaggg gaactccccc ggggcggatt
    39601 gaaggggaag gggggttctg ataaagtgaa agaatgagcc cgaacgagga acggaacgga
    39661 gaccaggcgt tcgagatcgc ctgtccgcat tgcggaacgg tgatttgggt ggacgggaga
    39721 aaccggagca tcctgaagac cgagcgggcc gcccggaaaa aagaatccct ggacgacctt
    39781 ctcgcccggg agaagaccaa gaaggacggc ctggccacaa aattcgaggc caccgcctcc
    39841 ctccagaaac aaaagaaaga ccgggccaag gacgcgttcg cgaaagcgtt cgccgcggcc
    39901 ggcgaggagc cgccggctga ggattagccg gcggtcaggc ctcgtcccgc gggccgccgt
    39961 cttcccgggt catggtgaac ccggtcaagc cgaagaccgc aagaatgaag agggcggcgt
    40021 aattcccgat cgcccagggc aggtaatcga gcaccgggac gccgagggct ccggcgacat
    40081 acacgccggc catgctccag gggacgagag ggacgatgat ccccccgcat tcgtcgatga
    40141 tccgggaaag gttcttgggc gccagccgca atttccggaa agcgggcccg agcagctctc
    40201 cgggcaggat gaccgccaga taagagctgc ccgtcactaa ggctgtgacg acgcaggatc
    40261 cgatcgtcac caggacgatg ctccccgcct tgcgggcccc ggccatgact ttatccaaga
    40321 ccacgccgag catgccggtt ttctgcatga tcccgccgaa gcagaaggcg gtgatggcca
    40381 ccaactgcgt ctccatcatg ctcatcaggc cgccccggct gaggaggcgg tccacttccg
    40441 gaactccggt ggcggccgga taccccgtgt tcagagacgc ggcgaggtcg gctaagggcg
    40501 ctttctggaa gacggcggcc agaaggacgg cgatcagcga ggataagagc atgccgggga
    40561 tggtcggctt cttggcgaag gccagggcga agacgatgac cagcggcagg agcagccaga
    40621 cgttgaatgt gaaatgggtc cggagcgtgc cggtgatttt cgtcaggacc tccgaggaga
    40681 cggcctgcgc ttgggagcgg aatccgaaaa aaaagaacac gccgaggctg agcaaccagc
    40741 cgggaaccgc cgaccacatc atgtgcttga tgtgatcgaa caggtcggcg ccggcggtca
    40801 cggcggccag gttggggatg tctgaaaagg gggacatctt gtcgccgaag tacgccccgc
    40861 agacgacggc cccggccgcc gggccgaggg gaaggccgag gcccatggcg atccccatga
    40921 aggcgacgcc gaccgtgccg atcgtccccc agctcgtccc cgtcgccacg gccgtgaggc
    40981 tgcagacgaa acaggccgtg acgagaaaga atttcgggga gatcagcttg aggccgtaat
    41041 agacgaccat cgggatcgtc ccggcggcca tccaagcgcc gatcatgact ccgacggcca
    41101 gcatgatgag gatcgccggc atggccttgg cgatcccctc gacgatcccc ttctccatct
    41161 ccttccagga gtagcccaag ccccagccga ggagaccggt caaggccgcg gccgcgacga
    41221 gcaggacctg cggcttgatt tgatacacgc cgtagccgat gccgagcaga acggccatga
    41281 gaatcaaggg aaggagggag aggaacaagg agggcttttt caccggcgcc ggagaggtca
    41341 tgaggggaaa ttatcacgcc gcccgggcat ttacaagaac ccccggccgg atttagaatg
    41401 gcggcggatc cgccatgccg tcccctgaag aagagcttcg cctcctcctt cccgtga
//
LOCUS       DAOOXH010000012        38937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_875_length_38937_cov_7.771617, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000012 DAOOXH010000000
VERSION     DAOOXH010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 38937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38937
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_875_length_38937_cov_7.771617"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PKV64_02865"
     CDS             complement(<1..721)
                     /locus_tag="PKV64_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPN16057.1"
                     /translation="MRLPFRSHVPVAGLVLVLAAAAALFPGCSKVQAPENVVFITLDT
                     FRADHLGVLSEGRAATPVLDGLAARGILYENAWSPIPITLPAHKSIFYGRPPHVLETY
                     NNGQIAASRRDTPSFVQAFRKKGFDTAGFVSLGVLTREYGTAEGFDHYDDRFPDERWY
                     LTAEEVNRRVIPWLEARGGKPFFLWVHYSDPHDPYAPPDMPEDLALRINGRPAASLRL
                     NDYARHAVELDLKAGENVLVFE"
     gene            complement(916..3690)
                     /locus_tag="PKV64_02870"
     CDS             complement(916..3690)
                     /locus_tag="PKV64_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPN16058.1"
                     /translation="MLASKPRLIGGPRLAAAVLALTLALAPALSFGQLAASADIPFHK
                     TVLDNGLTVIVHEDHKTPIVAFNVWYHVGSKNEKPGKTGFAHLFEHLMFNGSENHNED
                     YFPLMEKIGATDLNGTTSEDRTNYFENVPATALEVALWAESDRMGHLLGAVTQAKLDE
                     QRGVVQNEKRQYENEPYALADELIAQACFPKGHPYSWTVIGSMEDLSAASLSDVHEWF
                     KTYYGPSNAVVVIAGDITPDEAIAKVKAYFGDIPPGPPLALPGRNIAKRTGEQRQTAQ
                     DRVPQARIYKIWNIPPWGDRDGLFLDLAASILADGKSSRLYNRLVYTDQIATQVSAYA
                     ETREIAGLFRIEAMVKPGVEPAAVEKAIDEELARFLKDGPTADELIRVKAQTIGGVIR
                     GMERIGGFGGKSDILATSQVFGGTPDAFKNNLAVYDAAGAADIKRAAQAWLSDGVYVL
                     TILPYPEMTVVPSSIDRAKIPAAGTPPDARFPKLERAVLSNGLQVILARRDAIPVVNF
                     NLLFDAGTASDQFSRPGTASLALNMLDEGTAKRTSLQISDDLAKLGATLYTGSSLDHS
                     SVRLSALKANLDPSLEIFADVVRSPSFPEKEFTRLQKQMLAGIQQEKAQPMAMAIRVL
                     PRLLYGEGHAYGTPLTGSGTEASVSAITLADLKAFHETWFKPNHAVLIVVGAAGMDEI
                     KPRLEKLFAGWKPGDVPQKNIGPVAPRTAPEIYLIDKPGSPSSIVFAGQLILPKANPE
                     EIAIETMNDILGGTFTSRVNMNLREDKHWSYGAGTVILDSKGLRPLIGYSAVQADKTM
                     ESIREMAKEFGDLIAARPPTPEELGRTRDGKVLGLAGQWETGAVIGGLIENMIAFGLP
                     DDYYETYGAKVRELSLDQVARAAKTVVQPQSMVWLVVGDRAKVEPALKTLGYGDVKVL
                     DGDGNPVKSQ"
     gene            4033..4641
                     /locus_tag="PKV64_02875"
     CDS             4033..4641
                     /locus_tag="PKV64_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPN16059.1"
                     /translation="MRTIVGEAEIKTEVKRSRFIGRSFIRSTPDEALAAVKTLRASEP
                     GATHHCWAYRLGRDGEQARYHDDGEPQGTAGPPILEVLKKNGVTNVLLVVTRYFGGVK
                     LGAGGLVRAYGDAAKRVLEKSGLKDLRWMVEAEAEVPYPLLGTFESFLAGEDAEVTGK
                     TYGEHVTIRLRLPAAREEALREYYAALTSGQSSLLILGRDFR"
     gene            4867..6543
                     /locus_tag="PKV64_02880"
     CDS             4867..6543
                     /locus_tag="PKV64_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPN16060.1"
                     /translation="MTTILPRSFRPAAAALLTLGFLCLGPIATRLQADPPLLTKAEAT
                     DFKATSRHADVRAFIDELQKRSPLVRIESIGRTAGGFDIPALIVADPPVASPEQLRRD
                     GRAVVYFQANIHAGEVEGKEACLMLARDIAAAAKRPWLEKIVLVIVPNLNADGNEKID
                     PRNRSYQPGPAEGVGLRTNGWNLDLNRDGMKLESPEISAVVRNVYLRWDPLIFVDCHT
                     TNGSFHQEVVTYGWSANPNVDREIRTYMSEAMMPAVEKINEDKHGVLTCGYGYFRDDK
                     DPDKGWTAMEFEPRFSTNYFALRNRFAILIENNVYAEYKDRVWGNYHYLESLLEYASA
                     HVDEMKALAAAADARSVRRGMNPSAGDGFGLEYDYRPLKNPITVYAYETEVIGTEGAW
                     PRRRSTGKLHVRTVPYYADGFATRSVPFPAAYLVPVTEPAVLDLLLRHGLAVERLTAP
                     VEADVETFKISEIKAPDRLYQGHRLNAVKGEYLPGKAAFPEGTLVISTAQPLGMLAAC
                     LLEPESGDGLVAWNYLDKHLSSSWGGGTVGTTVPIYRLLKPAVLPAEAVR"
     gene            complement(6737..9406)
                     /locus_tag="PKV64_02885"
     CDS             complement(6737..9406)
                     /locus_tag="PKV64_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPN16061.1"
                     /translation="MSGEDGPRIIRKIAVVRTEYLSARGIHANLECRVRGGRNVPPAG
                     ALSPEIDCTTVSGERLTIRVRDYDVRFLDRVASSEALGHLDLIVRARRGDIEAAAELR
                     RRTNALYLRIAARFAEPSIVQNDLVIPYTAAGEYPASVISQKGRILFDLSRRGLSTAD
                     FCLLSSAAYHLPPEERRKHLREAVHDLEILTGRKLENPDDPLLIAVRSALPEYFPGFM
                     PTYLNVGLTPAVFKGLPRRYGKEAAGLIRLNNRKTILEALDPEAFAPIEKEIHPSLHP
                     ARTRELTEAVEALIARRNPELLTSAAAQLEFFQEKAYAYYETHLDALRNFMDREIHYP
                     SLILQRMVCTVMDDQSYPGVLYSRHPRRGQGVFVQFARKVFGEDLMTGRLQPEERTFF
                     HREEAREEFPAIYHFWDRLNQLEEIFRGPVIVEFTGVHGTFTTLQVNAAELAGAGMVT
                     AVMDMHRAGRIGAGRVRELIKPYHIRQIESDAIEPKSLEVLPRLAQGMSVLPRAAVSG
                     RLYFSADRVRQAKAERPRENVILAKERFTPQDAVDLPVVSGICSLSPAAIHVVTAAQN
                     LGIPSLLSLESDGVRIDPDKREMINRDGRVVREGDWVTISSRWRRLFLGKAVYAPARL
                     LRFMAGEHVDLTPAERPRFEQLARDYAAYRDLMEKFGEAEFGSLQDLGHALRYGRLRD
                     SAKAGEFINRSFDLNEGRVIDHLFEATLGTHLINLAAFERLTPDRQIRLLRAALALGR
                     ERGIFGYEAGAFVIGGFVRPEWPGSFWRQFEPADVVGLINEWVLHQKYLVLMDAVDER
                     RLRRIREEIINHGLPRVLVTTGFVRGFMSLKLAGIRLPAENEDLDPQTSEVLACLGRP
                     YGDFFDYADERSLGVLRRLCEKESVPLPGPEDR"
     gene            complement(9403..10503)
                     /locus_tag="PKV64_02890"
     CDS             complement(9403..10503)
                     /locus_tag="PKV64_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN16062.1"
                     /translation="MAGKLLFIDDEEIVLRSCRRIFAKEDFQIDTALSGEEGLAKARE
                     GDYDVVVTDLKMPGIGGMEVLRTLRKERPEIIVVIFTGYANVETAREALKNGAFDYIP
                     KPFTPDEIKEVVRNAVKARAGNAEAKMIDLLAIVSHELKSPLATVQTTAETLYRGYFG
                     KLEPAQQKIVETILRNCAYLEDVIRSYIDLSKMEIDDLASFQKEMRIGAEAVAETTAV
                     PEIKDNLRRMPIVVEVKEDPPVLGDSQLLKIVVRNLVNNAIKYGREGTPVQVTLERKG
                     GEAVLTVRNEGVGISPEDIQNRLFKKFERLKQKGTEGVKGSGLGLYICKKIVDKHGGR
                     IWAEGEQGSWIAFHVALPTAAVGGPNQAGTEA"
     gene            complement(10524..12446)
                     /locus_tag="PKV64_02895"
     CDS             complement(10524..12446)
                     /locus_tag="PKV64_02895"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPN16063.1"
                     /translation="MRRRLFPSNIRFKLVVSLLLIVAVTGSLSLVIGVNIIDKNLVRE
                     ATESVRNSLAATTELYNEEVEKRSRIVQYVAKTSEIVQATMTGDRAYLREALGRIKTE
                     FGFDIVNVLNPDGTVLVRANNFDAWGDSVAHYRHIQWVLRNRKPAAGTGILGPENIRN
                     EGRNLAERTPIKAVPTPLSRRHESSIEDRALVMKAASPIMENGRMVGLLYAAVLLNNN
                     DSFIDRFRRLLFKEERNRGREVGSSTIFLEDVRVATTVMDSAGKRAIGTLVSDEVFRN
                     VFEEGRTWIGPAFVVDSWYISGYAPLKDIDGRPVGMLYAGVLKDKYDDVLRTTAVLYL
                     LVIGLTTLIAIVLSIYLVGLWTRPVRRIMAATQDIADGNYHRIETLPKDDDDTRKIAQ
                     GFNVMAAAIEERDRELQAMAERTILKSEKLASLGRLASGIAHEINNPLTGVLTYSSLL
                     LEDLQGTEYAKDLEVIRDETLRCRKIVRGILDFARENVPEKILADINAVLDNALAILE
                     RNVHFQDIEIVREYDPDLPRIPVDVHQFTQVISNLAMNAADAMPGGGRLTLTTSFDRE
                     KRRIILRIADSGTGIPPEHQVRLFEPFFTTKDKGKGTGLGLPVVYGIIRRHNGTIDVK
                     SAVGRGTEFTITLPAD"
     gene            complement(12457..12861)
                     /locus_tag="PKV64_02900"
     CDS             complement(12457..12861)
                     /locus_tag="PKV64_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN16064.1"
                     /translation="MAKKILIVDDDKDLVESLSQVLQSRGYETAAAYSAAEGLKTLLA
                     EKPDLVILDVMMETDTAGFEAAGQIRSARPFSRYRDVRTVPLIILTAIGQVTNSRFSL
                     DPGDSFLPGVNAFLTKPVDLDELVQKIAALLG"
     gene            complement(12871..13410)
                     /gene="nuoB"
                     /locus_tag="PKV64_02905"
     CDS             complement(12871..13410)
                     /gene="nuoB"
                     /locus_tag="PKV64_02905"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HPN16065.1"
                     /translation="MAALFARAAAKAFPKSLWLYHCNSGACNGCDIEILNVLTPYYDV
                     ERFGIKLVGSPRHADVMLLSGAVTRPTLPLVKRAYEAIPAPKLVFGVGSCAAGGGCWF
                     DTYNVTGGGATAVPVDYLIPGCPPRPEAIIYGVALALGLVPQKSAPVELKQVEFPIDA
                     YLRNKAWEERNVIYKLIKD"
     gene            complement(13410..13958)
                     /locus_tag="PKV64_02910"
     CDS             complement(13410..13958)
                     /locus_tag="PKV64_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPN16066.1"
                     /translation="MWLTSKIKQIVLALSPGTVTLKYPFVKKPVPRGFRGQPVWDHTK
                     CIGCGGCANHCPARTILLRDLCQEIRVLLYDGSRCTYCGRCADLCPEKAIVMSPEFEL
                     AAGRREDVSQTIELFMLTCNRCGRCFDMEIKNAIDKLDLRGYRYDSLEARTVLRRTTD
                     RMAPEALAETEAYRRPEKIGGL"
     gene            complement(13975..14877)
                     /locus_tag="PKV64_02915"
     CDS             complement(13975..14877)
                     /locus_tag="PKV64_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPN16067.1"
                     /translation="MSYVIPFLNVILFLLLAPLFDGVLRKLTAKVQSRQGPPVIQPYF
                     DLFKLLGKERLDAAGTWPFRLAPVLAFAATLAVVAVIPFGGRAGVLAGWMDATVVIYL
                     LTLAGASVLFGALAGRNTFGLIGASREMITMIMVEPVLAMTLILGAVKIKSLSLAAAA
                     PAAAGAAFGLSSALMLVVYLLALQAFVARQPFDIAEAEVELLEGPFIEYSGPHLALFK
                     YAIMIKRMFYAWLFVSVFLPFLRTGVYALDILVQLLAMAVVFVLIALVGATNPRLRID
                     QAVRYYAVLIVAALAAVGLGVLGR"
     gene            complement(14874..16541)
                     /locus_tag="PKV64_02920"
     CDS             complement(14874..16541)
                     /locus_tag="PKV64_02920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPN16068.1"
                     /translation="MTPEKIFEVLKAEFGGAVGGSSPAAPGRLFIDIDPSRLRDAANR
                     LVGAGGRYQVGVGTDDRHRNGTLGLIHFFAFDGDHFTVGLRTAAPADHPDFDSITPDI
                     PAAGWSERECRDLLGMNFPGHPKPKKLVTADDWPDGIYPLRREVPYNLIPPAAEDVAY
                     QLDEAPPGTTIVPVGPFHATLHEPAHFAVYVDGETIKHCDYRGFMAHRGIEKLCQTQV
                     SYNEVPFIAERICGICGSVHSVAYCQAVEMAAGLAISRRAEFIRVVCLELERLHSHLL
                     LIGVAGHLIGYDTVFMHAWRIREQVMWLAEKITGNRKTYGMVIVGGVRRDITPEIKTE
                     TLAILKSLEAELVSFEKAIVGDTLIHRRTKGVGHVPKEEAVLWSLLGPTARGRGVDID
                     ARRDHPYAAYDELSFAVPVVPDGDVWGTIVVRLAELFESIKLIRQALERMPDGPVLTE
                     AAEALPALRPGWSMVEAPRGESFHYVLTGEENRPERWRVRAPTYTNLQGIPRMLQDNQ
                     FADFPIILGSIDPCFSCTDRVAVVDRRSGRGRVWSKKEMDRLSRSRS"
     gene            complement(16538..18346)
                     /locus_tag="PKV64_02925"
     CDS             complement(16538..18346)
                     /locus_tag="PKV64_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPN16069.1"
                     /translation="MSTLSTPISPALWAAFGLLLLAALVAPLLSRRRRAAGIVHLVLT
                     AAAAAVFLVLGAGAVFGRGMAAARTVSLGPIAVPILLDGLAGLFLVIVSFLAAMSAIY
                     AMRYADRFPAERRLGGFYAALPLFVLGMAGLVVVNDLSAGFTIAWQVMAVASFFLIRF
                     EKNAASVRAAGKFLVLMEAAWLLVIAGGLAAGGSLGESLQALGQKVQAMDGAGFVIVM
                     TLFVLGFGFLAGVFPLGRIWLPDAQAAAPAPANALLSGAMLKIGVFGLFRVFFCLAPA
                     AAAFNPCFWGPALAAIGVLTLFVGTLQSVKQSDAKRLLAFSSIGQVGYIVLALGVSLW
                     ASTASDPALRALAAVALLGALFHAVNHAAVKGLLFLISGNLHYAAGTKDLNKLGGLIT
                     LMPASAVIAAIASLSISGMPPTSGFASKETIISAALLSGAVSPVLVLFGIIALFTSAV
                     TLACYVKFFGLAFTASGAEETARKQIREVPLPMLLPEIILGILVILQGLAPSLFLGGV
                     SSAAERAAGLAAVPGIGEAAAGHFSALTPLLILGLLVVAILAAALLRRSAGSKTRTVP
                     TWLCGYQQLDGKNRYVDRGMFAALKHFFRWTGGGES"
     gene            complement(18346..19188)
                     /locus_tag="PKV64_02930"
     CDS             complement(18346..19188)
                     /locus_tag="PKV64_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HPN16070.1"
                     /translation="MDKRKNAAAVAVPVPAVLTEIRDEIEDVKTFYFRFEDPELERSF
                     SIRSGQFVMCTVFGAGEFAVSLPWSPEKDRRHLSVRNVGKVTSALHALGVGDKIGLRG
                     PFGNGFPFEEMAGRNLIYVAGGIGLIPLRSSIFHALLHREKFGRIILLYGAKSSKELM
                     YRPLIDEWRRTEGFETYVTIDREEPGWTGEVGFVHTLIEKAKVPVDGTTAFVCGPPVM
                     FNAVIGELSRRGLKDECIISTLERHMKCGIGKCQHCAIGRTLVCTDGPVYTYRQIKTL
                     GETI"
     gene            complement(19192..20250)
                     /locus_tag="PKV64_02935"
     CDS             complement(19192..20250)
                     /locus_tag="PKV64_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPN16071.1"
                     /translation="MYLLAKSDVPRFLSALSARFEMYGPQVHPVSGDVFFDKAADSSS
                     IRLDAPIPANSPKFAVFPHWERILSYAYDRETKDLRIVPEDLVRPKALVGIRSCDLTG
                     LLCFDRFFLGQEFVDEFYRAHRRRMFLVSVTCVTPFAQCFCVCTDSGPAAREGYDVNL
                     TDLGEAYLFEAGSDAGRELAASLSLPPAGPDAGRRRDEVIDRSISRFEAAAVENKAWI
                     SRAVNRLTTGFVKNETWEYIGQQCFECGACSFVCPSCSCFNIEDVNRRDGTTERLRTW
                     DSCSFEGYARMAGEHNPRRPVEDRRNKRFFCKLSFSQSKKYLRPGCVGCGRCVRVCPG
                     DIGMANVVTAIRREITGK"
     gene            complement(20253..22229)
                     /locus_tag="PKV64_02940"
     CDS             complement(20253..22229)
                     /locus_tag="PKV64_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPN16072.1"
                     /translation="MSYQTGICNFCGTGCGHMLQVEEGTIRGVFPSSSHPVGRGRLCG
                     RGWHIHELLNTEERIPRPLVRKGGGLTPVSWDEALDEAAARLGAFSGEEIGFWASPRS
                     SNEDAYALVRLARGVFRSANISVLSDVWYPTTAEVLREGTGRPGALGSIADIRKADFI
                     FVVGTDLTRQNPIIASDVHFAARGGADVVTLSTRLTQMAKLSGTHLQNKPGTKNIVMA
                     AMAKALLEDHPPDSASGAPAGFDGFRKSLEGLESAGLEARTGLPFDKIRDVARRLASA
                     RRAVIIFSTGITGLTRETTALIFNLFLAAGKAGREGCAILPVTGVSNLAGVNDMGAAP
                     DVLPGWRRLPDEAAASSFARSWGKDVNAGRGRSVESLLADASSGLKALVVVDHDPEIE
                     LLAGRIKSLEFVLYLGAYANPFKETAHVVLPTTTYAETDGTYTNTERRIQLNRRKIEP
                     RFEARPPWRIYADLAARRGQAWPYRSAADVQEEITRMIPEYHGAAHPALESRFGGWQW
                     PGGSGPDQSRLRFVPLNGAFSAAAEPAPAEFPFRLMAGRSYFYWHLNNIMKNTFIPRR
                     EYNALLLLYPRGLVEVHPDDAAALGLRDKRPVRVVSARGSMTPLARVSEEVPPGTVYA
                     PYFVGSMIPDFLVPHADAVEYGEDAVIPVRLEKV"
     gene            22498..26733
                     /locus_tag="PKV64_02945"
     CDS             22498..26733
                     /locus_tag="PKV64_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPN16073.1"
                     /translation="MKKSFRALALGPVLLCLAASAASGAVGRPGDFQFGHLTIFNGLS
                     QGSAMAMVQDERGFLWIGTEDGLNRYDGYGFKIYRPTAGPNGLSDSLINTLFLDSKKT
                     LWVGTMRGLNRYDRDRDDFTQFHPHPANPRSLSHDYITALAEDADGILWIGTAGGGLN
                     SFDPASGEFRAFLSRRAEPKTLSHDEILSLCRDAANYLWVGTADGLNRFNPRSGESVR
                     FNHDPGNPHSLSHNRVLALYQDGNGMLWVGTANGLNRFDPETGRFSRYFNSTRDPRSL
                     SDNYVTAVFRDRLKRLWVATQNGLNLYDEAADDFFVLRNDPTDPKSLAFDDVYSLFED
                     RTGVLWVGTRGRGIDKLAAENSRFALYQPRRGNPRSLGSGYVRAIAESPDGGVLYVGL
                     QDRGVDAIDRATGEYTHLRRVEGVDNSLNSELVYALAVDDSGTLWIGTYGGGLNSYDP
                     RTKTFRHYVHDPGDPQSLSYNDVRCLAVDGPDRLWVGTDGGGLCLLDLRARRFIRYRN
                     DPGDPASLSHNQVRSLHLDHAGTLWVGTFGGGLNRFNRETGGFAVWRHDPAKASATLC
                     GDFIPNMAEDSRGRLWIGTTAGLNLFNPADGTFECYTEEKNGLPNNAIYGVLVDEEDS
                     VWVSSNRGLARWNPQTRALKTYDISDGLQSNEFNGGARYRAKSGEMFFGGTSGLSAFF
                     PANLKDNPFRPEVVITNFEVFNKPVPVGGEVNGRILLDKSITETTDIDLRFRDRVITF
                     EFAALHYVAPEKNRYAYIMEGLDTGWQEVQGRRFVSYMNLDPGRYVFRVKASNNDGVW
                     NEEGTAVHLRVIPPFYMIRWIQALAVLLAGLAIYGAVRKRIRNIHRRTIELEEKVQER
                     TAELKKAEGEAREANRAKSEFLANMSHEIRTPMNGIFGMTELALETDLSPDQREYMEA
                     VKASADSLMTIINDILDFSKIEAQKIEMEKIPFHLRDTVHAAVTGVALLAERKSLELA
                     YSIPGTVPDGLIGDPGRFRQILTNLLSNAIKFTNEGEVVVSIDAARRADDQVWLRVQV
                     KDTGIGIPPDKQRLIFEAFSQADTTTTRMFGGTGLGLAISTQLVALMKGKLEVESEIG
                     RGSTFFFTVPFGLHHEEGKAVTPLRLSDLKDLPVLVVDDNATNRRILHEMLSHWGMRP
                     VTVDGAQAALLALQQARLEGRPFRLMLTDANMPEMDGFDLAARIKEEPHYKDMLIMLL
                     SSSGFRGDSSRCRDLGLAAYLTKPVKQSLLLDAVMMSLGDKEAPAGQARLITRHSIAQ
                     NRSRYSVLLAEDNIINQKLAVKILENRGHRVTVVENGEEVLDALEKGSFDLILMDVQM
                     PMMDGFQATREIRRREVATKAHQPIVAMTAHAMVGDREKCLEAGMDGYISKPLKPLDL
                     LRTIDEILARRPREEEEEPVLPVEEDNEEPRPS"
     gene            complement(26801..27433)
                     /locus_tag="PKV64_02950"
     CDS             complement(26801..27433)
                     /locus_tag="PKV64_02950"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HPN16074.1"
                     /translation="MTATASKPAGKRFRSRAFASFGLLWSFLAAAVSGIVMYFRPEGS
                     VAAWSGWSVLGLDKKSWEGVHTLIVVALMAFAVLHVILNWRALWGYLRRRASALSGAK
                     TECLAATLIVVVLTAGAVLRWKPLWRLMDARAAIKQGALAVTVSPPSAEAAGMSLAEL
                     CRSVPVSVEDAMERLARAGWRVDDPSRSLEDLAASFGASPERLYRALAGR"
     gene            complement(27430..27942)
                     /locus_tag="PKV64_02955"
     CDS             complement(27430..27942)
                     /locus_tag="PKV64_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16075.1"
                     /translation="MLGELYSRCPDAEAFRHAVEALGGDFVLDAAEMIELGEAYFRRH
                     PDTSCNRDRESVLAGYALVRLCVTERLIRRLSPAEREFYRGVFQNPGRVGVLSGRFSD
                     DELQAGLAAVEAAMAEIRESIEGIPKGPVKERFIGGISHLCTVLYLIRLHLDKRTPGL
                     DSGQSGGGKS"
     gene            complement(27984..28358)
                     /locus_tag="PKV64_02960"
     CDS             complement(27984..28358)
                     /locus_tag="PKV64_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN16076.1"
                     /translation="MSDPKKVLIIDDDPAICDSVKAVLEAVGFQTEVAYSGKDGVAAF
                     RRSRPDVVLCDMMMEDLDAGAQAAKIMRKERPDVPIFLLSSIGDATAETVGFGHLGFS
                     GIFQKPVNFDLLLAVMGRIAKS"
     gene            28496..31327
                     /locus_tag="PKV64_02965"
     CDS             28496..31327
                     /locus_tag="PKV64_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN16077.1"
                     /translation="MNTGRRISRAERLWGGLLAAALLAGTAAASQTGEIRGKVLDESG
                     LGLPGVEIRAASPALQGARTVFSGRDGSFHFPLLPVGTYSLTFKLAGFTTIVQEAAIV
                     RLGLTTTLTVRMPQAALETEVVVTAETPLIDRSSTDTSTRLGAADLEKIPAQNRTVVD
                     VVKFTPGAAGIRADTRRGTSTEGQPSFRGAGAEGNNWVIDGLAVSGVRMKDSGVNLNY
                     DSLEEIQIISDPFSPEFGSSYGGIINMVTKSGGNRFHGEASLLIENRHLQAARQSQLS
                     VVSEPDAFNNTSAYVNLGGPILKDKLWFFLSNDYQSLTEETKEAAFGYLAVPGGRRTI
                     GRDNIFVKLSFAPHADHGLSLTFLRDQSFPQRGGIGLPEMNEEKRVRDAMFRLNYRGI
                     LSPRTFVEAGLGRVRRDLDTTPVDGDLGPAMYYVKDLARNVHNSYGNVIDDERRFDAQ
                     VKMTTYFETPRFGRHEITAGFEYYDVSSDFEVDFSGKNEDLFPGNGFDVGTKYIFETR
                     KGGVGVPSGFYEYGPFDLINASRGIGLFLKDKVSWDRFTLMAGLRAQTQACLSDTGET
                     LWSWGLGDFLSPRVSFTADLTKDGRNVLKLAWGRFSDMITTMALGFFNSGMVPTYRLY
                     RWAGPESPGEADVHNPDFWAFEIQQSQKFDINPRLKPDFQTRWLVEFDRRIGPSWAVR
                     ARFVRTTADNLLELLMIFDPRTIYRFLYDNFEYKRRDYTGLELEITGSIGRRLFLNAS
                     YARALARGTNPGQSETGRWSEEEGSTYYLGMFGNHIYVPDMPELAALKAWADAALAGL
                     GGRNIGDEGWYGRLPYSVDHDVKLNAVYSAPAGIQVAAAFEFISGYPWEKTGYVPFFG
                     GYYAFPEGRGARTTPAHSFLDVSIEKSFALPGSGIFADAAVAVRLDVFNVFNSQRPVS
                     YVKEDTPLFGDVWGRQQPRQARLSAKIKF"
     gene            complement(31331..33160)
                     /gene="glmS"
                     /locus_tag="PKV64_02970"
     CDS             complement(31331..33160)
                     /gene="glmS"
                     /locus_tag="PKV64_02970"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPN16078.1"
                     /translation="MCGIVGYIGPKDVVPVLLEGLKKLEYRGYDSAGVAVAGGGGIRL
                     CRVRGKVAALEECLGRTPIEGAYGLGHTRWATHGCPSEANAHPHVDCTGGIVVVHNGI
                     IENYLALKTKLKAEGHVFRTETDTEVIAHLVEKHFSGSLEEAVRAALAEMEGAFAVGA
                     IAVKDPGRIVAAKMGPPLVIGLGDGETIVSSDINPILAHTSRVAFLEDREMAVIDASG
                     PRFLDFKGAPVEKKIERISWSPLMVEKQGFKHFMLKEIYEQPQVIRDTLMGRISLDRG
                     EVLLAETGIPAEVFRDVRKAVIIACGTSSHAGLIGKYFLESLAGIPADVEYASEYRYR
                     DFILDKDALVIVISQSGETADTLAALRAAKGRAAATLAVTNAVSSSIARESRGVLYTH
                     AGPEIGVAATKTFTAQLTALALVALGLAQAKGAGTKEDRLAVIAELQRIPHKVERILH
                     RAGAIEDLAQQFMAFSHFLYLGRWVNYPVAMEGALKLKEISYIHAEAYAGGEMKHGPI
                     ALIDEKMPTMALIPRDRVYDKMRSNIAEIKARAGFVIAAADENDAALREHVDAVIPLP
                     ATHPLLTPFLAVVPLQLFAYQIAAKRGADVDQPRNLAKSVTVE"
     gene            complement(33210..34589)
                     /locus_tag="PKV64_02975"
     CDS             complement(33210..34589)
                     /locus_tag="PKV64_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPN16079.1"
                     /translation="MDNLLIGLISAGGEDEGGFKSTTPKALHSLLGMTLLEWAAAAAA
                     GAGAKRLVVAGLDPEAVKTGIPGLSAEVVAAKAKAGNPAAIVQSARKEWAGNAAADVL
                     ILPADLPLIPPAVLRNLVSRHRRKKYAGTMLWAESAEIPPSSARIPILEDRGAIPRRV
                     PTGVFVFQAGALDRVLPRAARTGRKGGSDLTAAFGLLAAVGNPVGVVLAPEPEDVRRI
                     QTRFDLGQAADRLRLRKIRDLAEHGVTILSPATTWIDWNVRVGAETVIYPGAVIEGPT
                     IIGRNSRIHPHARIVRSVLGDNVHVLGSSEIEDATVADDVRIGPFARIRMQTSLRAGS
                     RVGNFVEMKKTDFGPGSKAMHLSYLGDSEVGAAANIGAGTITCNYDGVRKNRTVIGAK
                     AFIGSGSELIAPVRIGRGAYVAAGSVITKDVADDALAVARGRQVEKPGWAAVRRAKLQ
                     AGKNGGSKS"
     gene            complement(34608..35120)
                     /locus_tag="PKV64_02980"
     CDS             complement(34608..35120)
                     /locus_tag="PKV64_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN16080.1"
                     /translation="MKEEKSKKDEYQKALTAYGDAMKEFRKSKWDKAQESFGAFIEKF
                     PAERDLVARARTYQSIAAERFKEPREIPALKTAEDYVSAAVYKMNTGAAEEALKLVEK
                     ALKSDPADARLLYLQADLLCRRGRLDESLEALRQAVAGEKAYRILAQNEVDFAPLWED
                     KRFKAITKTS"
     gene            complement(35372..36220)
                     /gene="lpxC"
                     /locus_tag="PKV64_02985"
     CDS             complement(35372..36220)
                     /gene="lpxC"
                     /locus_tag="PKV64_02985"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HPN16081.1"
                     /translation="MTRSKTLAAPVFFEGVGVHSGRSVRLRLLPSSIPGIIFRRTDLD
                     NAELPLTTGRIDSQNSTTLVGDRFRVRTVEHLLAALWVPGIDSLTIELDADEIPIMDG
                     SAEPFARALQTAGIERLPDFQPFLRIVRPFALADEGAAIDFAPPIEDGGLELSYTIDY
                     PHPAIGGQTLRRPIRWGVFVREIAPARTFGFLKDAESLRSRGLALGASAENTILMDDE
                     SVMNGPLRFPDEFVRHKLLDLIGDLALLGRPLAGRVSAVKAGHRLHFAAVRHLLDHPE
                     LTVLGE"
     gene            36394..37134
                     /gene="larB"
                     /locus_tag="PKV64_02990"
     CDS             36394..37134
                     /gene="larB"
                     /locus_tag="PKV64_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HPN16082.1"
                     /translation="MKTQIEDLLTKVRRGTLTPRKALAALKDYPFQDLGFAKVDHHRE
                     IRKGYPEIVYGLGKTDSQIVQIAAAIVQKGSNLLITRVEASTYAAVKEAVPKAAYNAL
                     GKIIYLRKRPAPPGRGMIAVLTAGTSDIPVAEEAAVTCRMLGNRVQRFYDVGVAGLHR
                     LLSEYGRLKKARVIIVCAGMEGALPSVVAGLFSVPIVAVPTSVGYGASFKGVAALLAM
                     LNSCPGGVGVVNIDNGFGAAYLASLINR"
     gene            37336..>38937
                     /gene="dnaG"
                     /locus_tag="PKV64_02995"
     CDS             37336..>38937
                     /gene="dnaG"
                     /locus_tag="PKV64_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPN16083.1"
                     /translation="MEILDQVRNAANIVEIAGQYTALRQRGKKHVGLCPFHSEKTPSF
                     TVDADKGLYHCFGCGAGGDIFTLVMEKENLDFPEAIAFLAEKYHIPLPEKKRLSPQAL
                     KLEEQLRKVLEGALSFFRKSLRETAEGKKALEYLRGRGLSAAVIEEFALGYAPNSWDA
                     LIKYFKAKGVAPALLEKAGLVVPGRKQGEFYDRFRGRAIFPIFTVTGKAVAFGGRSLF
                     GQDPKYLNSPDTPLYSKGHLLYGLNVTKDAIREAGEFILVEGYTDFLSLYQAGRRNVV
                     ASLGTALTSRQVGLAMRFAPKAVINYDGDPAGRTAAFRAVPLFFEKGVETRVLILPEN
                     LDPDGFLRKNGAAAYDLLYPGAAPGLKFIIDYLSREKRLDVPEVKTRVLRAVMDIVEA
                     VPDAIVRSEYFRLTAEHLGVDESLLRSLSQVKPGEAAARPAETFLPAEKRLLQILMEN
                     ADLRPDIFSELCEEDIAGLKSEPIFAIMLRLFANEQDFSLNEMHKSVEPGLARELSQA
                     LLERREAPSLQEALDCLWALRVAGKE"
BASE COUNT         7081 a        12433 c        12673 g         6750 t
ORIGIN      
        1 cttcaaaaac gaggacattc tcccccgcct tgaggtcgag ctcgacggca tgccgggcat
       61 aatcgttcag gcgaagcgag gccgccggcc ggccgttgat ccggagggcg aggtcctccg
      121 gcatgtccgg aggagcatag ggatcgtggg gatcggaata atgaacccag aggaaaaacg
      181 gcttgccgcc gcgggcctcc agccagggga tgacccggcg gttgacctct tcggccgtca
      241 agtaccagcg ctcatccgga aaccggtcat cataatggtc gaatccctcg gccgttccgt
      301 attcgcgcgt caggactccc aaggagacga atccggccgt gtcaaaaccc ttcttccgga
      361 aggcctggac aaaggaaggg gtgtcgcgcc gggaggcggc gatctggccg ttgttgtacg
      421 tctccaggac gtggggaggg cgcccgtaaa agattgattt gtgagcgggc agcgtgatcg
      481 gaatcgggct ccaggcgttc tcgtagagaa tgccccgggc ggccaagccg tccaggacgg
      541 gcgtggccgc ccggccttcc gaaaggacgc ccaggtgatc ggcccggaac gtgtcgaggg
      601 tgatgaaaac gacattttcg ggcgcctgaa ccttcgaaca tccggggaac agcgccgccg
      661 ccgcggctaa aaccaaaacc aggcccgcga ccgggacgtg acttcgaaac ggaaggcgca
      721 aggacaatcc tttgatgtaa attccctttc attatgtcga aaacggaaaa gagtgtcaaa
      781 agccgcggaa aaatctccgg aagaaggccg tcaagaagag gaaacgatat aagccggggc
      841 gatccccccc ttgaggggga ggatcgcccc tttttccaaa cttcgttcca atccggccga
      901 agccggcgga ctttgttact gtgatttaac cgggttcccg tcgccgtcca ggaccttcac
      961 gtctccatag cccagggtct tgagggccgg ctcgaccttg gccctgtcgc cgacgacgag
     1021 ccagaccatg ctttgaggct gaacgacggt cttggccgcg cgggcgacct ggtcgagcga
     1081 aagctccctg accttcgctc cgtatgtctc gtagtaatcg tcgggcaggc cgaacgcgat
     1141 catgttctcg atcagtccgc cgatgaccgc ccccgtctcc cactggccgg ccaggccgag
     1201 gactttgccg tcccgggtcc gcccgagctc ctcgggcgtc ggaggccggg cggcgatgag
     1261 gtcgccgaac tccttggcca tctcccggat ggactccatg gtcttgtcgg cttggaccgc
     1321 gctgtagccg ataagcggcc ggagaccctt ggaatccagg atcaccgttc cggcgccgta
     1381 ggaccagtgc ttgtcctcgc ggaggttcat gttgacccgg gaggtgaagg tcccgcccag
     1441 gatgtcgttc atcgtctcga tggcgatctc ctcgggattg gccttgggca ggatcagctg
     1501 gccggcgaag acgatcgagg agggcgagcc gggcttgtcg atcaagtaga tttccggcgc
     1561 cgtcctcggg gcgaccggcc cgatgttctt ttgcggcacg tcgcccggct tccagccggc
     1621 gaacagcttt tcgagccggg gcttgatttc atccatgccg gccgcgccga ccacgatcag
     1681 gacggcgtga ttgggtttaa accaggtttc atggaaggcc ttgaggtcgg ccagggtgat
     1741 cgccgagacg gaagcctcgg tccccgagcc ggtgagcggc gttccatagg cgtgtccctc
     1801 gccgtaaagg aggcgcggca ggacgcgaat ggccatggcc atcggctggg ccttttcctg
     1861 ctggattccg gccagcatct gcttctggag ccgggtgaat tccttctccg ggaaggaggg
     1921 cgaacggacg acgtcagcga agatttcaag cgagggatcg aggttggcct tgagggccga
     1981 aagccggacc gaggagtggt ccagggacga accggtgtag agggtcgccc cgagcttggc
     2041 caggtcatcg ctgatctgga gcgacgtccg cttggccgtg ccctcgtcga gcatgttcaa
     2101 ggccagggag gccgtgcccg gccggctgaa ttgatccgag gccgtgccgg cgtcgaaaag
     2161 gaggttgaag ttcaccaccg ggatcgcgtc ccggcgggcc aggatgacct ggaggccgtt
     2221 ggacaagacg gcccgttcga gcttggggaa ccgggcgtcg ggaggcgtcc cggccgccgg
     2281 gatcttcgcc cgatcgatcg aggacgggac gaccgtcatt tcgggatagg gcagaatcgt
     2341 cagaacgtat acgccgtctg agagccaggc ctgggccgcc cgcttgatgt cggccgcgcc
     2401 cgccgcgtca tacacagcca ggttgttctt gaacgcgtcg ggcgttccgc cgaacacctg
     2461 ggaggtcgcc aggatgtcgc tcttgccgcc gaagccgccg atccgctcca tcccccggat
     2521 gacgccgccg atggtctggg ccttgacccg gatcagctcg tcggccgtcg ggccgtcctt
     2581 gaggaagcgg gccagctcct cgtcgatcgc cttttcgacc gcggccggct cgacgccggg
     2641 cttgaccatc gcttcgatcc ggaagaggcc ggcgatttcg cgcgtctcgg cgtaggccga
     2701 gacctgggtg gcgatttggt cggtgtagac gaggcggttg tagagccggg acgatttgcc
     2761 gtcggccagg atcgaagcgg ccaggtccag gaagaggccg tcccggtcgc cccagggcgg
     2821 aatgttccag attttgtaga tccgggcttg ggggacgcgg tcctgggcgg tctgccgctg
     2881 ctcaccggtc cgcttggcga tgttccgtcc gggcagggcg agcggcgggc cgggcgggat
     2941 gtcgccgaaa tacgccttga ccttggcgat cgcctcgtcc ggggtgatgt ccccggcgat
     3001 gaccaccacg gcgttggacg ggccgtagta cgtcttgaac cattcgtgga catcggaaag
     3061 cgaggccgcc gacaaatcct ccatcgagcc gatgaccgtc cagctgtagg gatgcccttt
     3121 ggggaaacaa gcctgggcga tcagttcgtc ggccagggcg tagggctcgt tctcgtactg
     3181 gcgcttctcg ttctggacga cgccgcgctg ctcgtcgagc ttggcttggg tgaccgcgcc
     3241 caggaggtgg cccatccggt ccgattcggc ccaaagggcg acctcgaggg ccgtcgcggg
     3301 gacgttctca aaatagttcg tccggtcctc gctggtcgtc ccgttgaggt cggtcgcccc
     3361 gatcttctcc atcaagggaa aatagtcctc gttgtggttc tccgagccgt tgaacatcaa
     3421 gtgctcgaaa agatgggcga atccggtttt tccgggtttc tcgtttttcg agccgacatg
     3481 gtaccaaacg ttgaaggcca cgatcggggt tttgtgatcc tcgtggacga tgacggtcag
     3541 gccgttgtcc aggaccgtct tgtggaaggg aatgtcggcc gaggcggcca gctgtccgaa
     3601 ggacagggcc ggggccaagg ccagggtcag ggctaagacg gcggccgcca gccgcggtcc
     3661 gccgatcaac cggggttttg acgcgagcat cggttcctcc tcgatatagc aaataggcgt
     3721 gaatattcat tatatacgtt tttacccgct tcgggttatc gcccttcgcc ccggcgcgga
     3781 cgagggagga tccgtcccct cgaggattcc ccggtccgcc gacgactccc gatttccgcc
     3841 gaatccgggc cggatccgcg gattttccgc atcggtcttt tccttttttg ttttaaaatc
     3901 gggaaacggg cgagccggcg ggatcggccg tccgcgggat gtgaaatcgg gagacacgga
     3961 aagacctctt cgcccgaaat caaggagagg gggcttgtgt ctcctgattt cttttgctac
     4021 gttgagaccg gcatgcgaac gatcgtcggc gaggcggaaa tcaagaccga ggtcaagcgg
     4081 tcgcggttca tcggccggag cttcatccgc tcgacgccgg acgaagccct ggccgcggtc
     4141 aaaacgctcc gcgcgtccga gcccggagcc acccaccact gctgggccta ccgcctcggc
     4201 cgggacgggg aacaagcccg ctaccatgac gacggcgagc cccagggcac ggccggcccg
     4261 cccattctcg aggtcctgaa aaagaacggc gtcaccaacg tcctgctcgt cgtcacccgc
     4321 tatttcggcg gcgtcaagct gggggccggc ggcctcgtcc gggcttacgg cgacgcggcc
     4381 aagcgggtgc tggaaaaatc ggggctcaag gatcttcgtt ggatggtcga ggcggaggcg
     4441 gaagtcccct atcccctgct cggcacgttc gaaagcttcc tggccggcga ggacgccgag
     4501 gtcaccggaa agacgtacgg cgagcatgtc acgatccggc ttcgcctgcc cgcggcccgc
     4561 gaggaggcgc tccgggaata ttacgccgcc ctcacttccg gccaatcctc tctcctcatc
     4621 ctcggccggg atttccgctg agccggccgg tcgcgtcgag ggatccgttc ccgcggcctc
     4681 cacccgcccg ggcgcccgga acacctgtcg aagctccttc acccggcgcg ggatcccggg
     4741 aatcggggaa tcttccgccg cgcaccggtg agaacctcgg ggatggggat tcattcccgc
     4801 gtttgacaga cttgaccgga tggcctattt taagccttcg acgaaattct tttaaggata
     4861 gaggccatga ccacgattct cccgcgttcc ttccggccgg cggccgccgc tcttctgacc
     4921 ctcggctttc tctgcctcgg gccgatcgcg acgcgcctcc aagccgatcc gcctctcctg
     4981 accaaggccg aggcgacgga cttcaaggcc acctcgcgcc acgccgacgt ccgggccttc
     5041 atcgacgagc tgcagaaacg cagcccgctc gtccggattg aatccatcgg ccggacggcc
     5101 ggcggcttcg acatccccgc cctcatcgtg gccgacccgc cggtcgcttc gccggaacag
     5161 ctgcgccgcg acggccgggc cgtcgtctac ttccaggcca acatccacgc cggcgaggtc
     5221 gagggcaagg aggcctgcct gatgctggcc cgggacatcg ccgccgcggc gaagcgtccc
     5281 tggctggaaa aaatcgttct ggtcatcgtc cccaacctca acgccgacgg caacgagaaa
     5341 atcgaccccc gcaaccgctc ctaccagccc gggccggccg agggcgtcgg tctccggacc
     5401 aacggctgga acctcgacct caaccgcgac gggatgaagc tggagagccc ggagatctcg
     5461 gccgtcgtcc gcaacgtgta cctgcgctgg gatcccctga tcttcgtcga ctgccacacg
     5521 accaacggct ccttccacca ggaggtggtc acctacggct ggagcgccaa ccccaacgtc
     5581 gaccgcgaga tccgaactta tatgagcgag gccatgatgc cggccgtcga gaaaatcaac
     5641 gaggacaagc acggcgtcct gacgtgcggc tacggctatt tccgcgacga taaggatccg
     5701 gacaagggct ggacggcgat ggaattcgag ccccgcttct cgaccaatta tttcgccctg
     5761 cgcaaccgct tcgccatcct gatcgagaac aacgtctacg ccgaatacaa ggaccgggtc
     5821 tggggcaatt atcattatct cgagtccctc ctcgaatacg cctcggccca tgtcgacgag
     5881 atgaaagccc tggccgcggc ggccgacgcc cggtccgtcc ggcggggaat gaacccgtcc
     5941 gccggcgatg ggttcggctt ggaatacgat taccgtcccc tcaagaatcc catcaccgtc
     6001 tacgcctacg aaaccgaagt catcgggacc gagggcgcct ggccccggcg ccgatccacg
     6061 ggcaagcttc atgtccggac ggttccctat tacgccgacg gcttcgccac ccgctcggtt
     6121 ccgttcccgg ccgcctacct cgtcccggtg accgagcccg ccgtcctcga cctgctgctc
     6181 cgccacggcc tggccgtcga gcggttgacc gccccggtcg aagcggacgt cgagacgttc
     6241 aagatcagcg agatcaaggc gcccgaccgg ctctaccagg gccaccggct gaacgcggtc
     6301 aagggcgagt accttccggg gaaagcggcc ttcccggagg gaaccctcgt catctccacc
     6361 gcccagcctc tcggcatgct ggccgcctgc ctgttggaac cggaaagcgg agacgggctg
     6421 gtcgcttgga actacctcga caagcacctg tcctccagct ggggcggggg gacggtcggg
     6481 acgaccgttc cgatctaccg gctcctgaag ccggcggttc tgcccgcgga agccgtccga
     6541 taggcccgga gcctgtcccc ggccggcgga cggattcccc ggggggagga atccgcggag
     6601 cattcgcgcc gattcaaaat aatccggcgc cggccgcccg ccgcttcaaa gacgacggga
     6661 aggatcggac cggtcgcgac gatgaacacc gccccggaca cctctcgaag cgcgaggacc
     6721 cggcggtctt tctttctcag cggtcctccg gccccgggag cgggacgctc tccttttcgc
     6781 aaagccgccg gaggacgccg agactccgct cgtcggcgta atcgaaaaag tcgccgtacg
     6841 gccggccgag acaggcgagg acctcggacg tttgagggtc gagatcctcg ttttccgccg
     6901 gaagcctgat tccggcaagc ttcagggaca taaagccccg gacgaatccg gtcgtgacca
     6961 ggacccgggg caggccgtgg ttgatgattt cctcccgaat ccgccgcagg cggcgctcgt
     7021 cgaccgcgtc catcaggacc aggtacttct ggtggagaac ccactcgttg atcagcccga
     7081 cgacatccgc cggctcgaac tgccgccaga acgatcccgg ccattccggc cggacgaatc
     7141 ccccgatgac gaaggcgccg gcctcgtacc cgaaaatccc ccgctcccgg ccgagggcga
     7201 gggcagctcg aagcaggcgg atctgcctgt ccggcgtcag ccgctcgaaa gccgccaggt
     7261 tgatgaggtg cgtcccgagg gtcgcctcga acagatgatc gatcacccgg ccctcgttga
     7321 ggtcgaagct gcggttgatg aactccccgg ccttggcgct gtcccggagc ctcccgtaac
     7381 gaagagcgtg gcccaggtcc tggaggctgc cgaactcggc ctctccgaat ttttccatga
     7441 gatcccggta cgccgcgtag tcccgggcca gctgctcgaa ccggggccgc tcggcgggcg
     7501 tcaggtcgac gtgctccccg gccatgaagc gcagcaggcg ggccggggcg tagacggctt
     7561 ttccgaggaa caggcggcgc cagcggctgg aaatcgtgac ccagtcgcct tcccggacga
     7621 cccggccgtc ccggttgatc atctcccgct tgtccggatc aatccgaacg ccgtcgcttt
     7681 cgaggcttaa aagcgaggga atgcccaggt tttgagcggc ggtcacgaca tggatcgccg
     7741 ccgggctcag gctgcaaatc ccgctgacga ccggcagatc caccgcgtcc tggggggtga
     7801 agcgctcctt ggccaggatg acgttttccc gaggccgttc ggccttggcc tggcggacgc
     7861 ggtccgccga gaaatagagc cggcccgaga cggccgcccg gggcaggacc gacatcccct
     7921 gggccagccg gggcaggact tccaaggatt tcggttcgat ggcgtcggac tcgatctgcc
     7981 ggatatggta gggcttgatg agctcccgga ctcggccggc cccgatccgg ccggcccggt
     8041 gcatatccat caccgccgtc accatcccgg ccccggccag ctcggccgcg ttgacctgga
     8101 gagtcgtgaa cgtcccgtgc acgcccgtga actcgacgat gaccggtccc cggaagatct
     8161 cctccagctg attgagacga tcccagaaat gatagatggc cggaaattcc tcccgggctt
     8221 cctcccggtg gaagaacgtc cgctcctcgg gctgaaggcg gccggtcatc aggtcttccc
     8281 cgaacacctt tcgggcgaac tggacgaaga cgccctggcc ccggcggggg tggcggctgt
     8341 agagaacgcc cggataggat tggtcgtcca tgacggtgca gaccatccgc tggaggatca
     8401 gggacgggta atggatctcc cggtccatga aattccggag ggcgtccagg tgggtctcat
     8461 aataggcgta ggccttctcc tggaagaatt cgagctgggc ggccgccgac gtcaggagct
     8521 ccgggttgcg gcgggcgatc aaggcctcga cggcctcggt cagctcccgg gtccgggccg
     8581 gatggagaga cggatgaatc tccttttcga tcggggcgaa ggcctccgga tcgagggcct
     8641 cgaggatcgt tttccggttg ttgagccgga tcaagccggc ggcttccttc ccgtagcggc
     8701 ggggaagccc tttgaatacc gccggagtca ggccgacgtt caggtacgtc ggcatgaatc
     8761 ccgggaagta ttcgggcagg gccgagcgga cggcgatgag gagcgggtcg tccggatttt
     8821 cgagcttccg gccggtcagg atctccaggt catggacggc ttcccggaga tgtttccggc
     8881 gctcctccgg gggtagatga taggccgcgg acgaaagcag gcagaaatcc gcggtcgaca
     8941 ggccgcgccg ggacaggtcg aacaggatcc gccccttctg gctgatgacc gaggccggat
     9001 attcgccggc cgcggtatag gggatcacca ggtcgttttg gacgatggac ggctcggcga
     9061 accgggccgc gatgcggaga taaagagcgt tcgtccggcg ccgcagctcg gcggccgctt
     9121 cgatgtctcc cctccgggcc cggacgatga ggtccaggtg gccgagggcc tcgctggagg
     9181 cgacccggtc caaaaaccgg acgtcgtaat cgcggacgcg gatggtaaga cgctcgcccg
     9241 agaccgtggt gcagtcgatt tccggcgaga gggccccggc cggaggaacg ttccggccgc
     9301 ctcggacgcg gcattcaagg ttggcgtgga tgccccgggc gctgaggtac tcggtccgga
     9361 cgacagcgat cttccggatg atccgcgggc catcctctcc gctcatgcct ccgtcccggc
     9421 ctgattcgga ccgccgacgg cggccgtcgg cagggcgaca tggaaggcga tccaggatcc
     9481 ctgttcgccc tcggcccaga tccggccgcc gtgcttgtcg acgatcttct tgcagatgta
     9541 gaggccaagg cccgagccct tgacgccctc ggtgcccttc tgtttcagcc gctcgaattt
     9601 cttgaagagc cggttctgga tgtcctcggg ggagatcccg acgccctcgt tgcggaccgt
     9661 caagaccgcc tctccgccct tccgctccaa ggtcacctgg acaggcgtcc cttcccggcc
     9721 gtatttgatc gcgttgttga ccaggttgcg gacgacgatc ttgagcagtt gggagtcgcc
     9781 caggacgggc ggatcctctt tcacctcgac gacgatcggc atccggcgga gattgtcctt
     9841 gatctcgggc acggccgtcg tttcggccac ggcctcggcc ccgatccgca tctccttctg
     9901 gaaggaggcc aggtcgtcga tctccatctt ggacaggtcg atgtaggacc ggatgacgtc
     9961 ctccaggtag gcgcaattgc ggaggatggt ttcgacgatt ttttgctggg ccggctcgag
    10021 cttgccgaaa tacccgcgat agagcgtctc ggccgtcgtc tggacggtgg ccagggggct
    10081 tttgagctca tgggagacga tggccaagag gtcgatcatt ttcgcctcgg cgtttccggc
    10141 ccgggctttg acggcgttgc ggacgacctc cttgatctcg tcgggggtga acggcttggg
    10201 aatatagtcg aaggccccgt ttttcaaagc ctcccgggcc gtctccacgt tggcgtagcc
    10261 ggtgaagatg acgacgatga tctccggccg ctccttccgg agggtccgga gaacttccat
    10321 ccccccgata cccggcatct tcaggtcggt gacgaccacg tcgtagtcgc cctcccgggc
    10381 cttggccagg ccttcctcgc cggacagggc cgtgtcgatc tggaaatcct ccttggcgaa
    10441 aatcctccgg cagctccgca ggacaatctc ctcgtcgtcg atgaaaagca gttttcccgc
    10501 cataatccta tcctttgcgc ccgtcaatcg gcgggcagcg tgatcgtgaa ctcggtgccc
    10561 cggccgacgg cgctcttgac gtcgatcgtc ccgttgtggc gccggatgat gccgtaaacg
    10621 acgggcaggc ccaggcccgt tcccttgccc ttatccttcg tcgtgaagaa cggctcgaac
    10681 agccggactt ggtgttccgg ggggattccc gtgccggaat cggcgatccg gaggatgatc
    10741 cggcgcttct cccggtcgaa cgaagtcgtc agcgtcagcc gcccgcctcc cggcatggcg
    10801 tcagccgcgt tcatggccag gttgctgatc acctgcgtga actggtggac atcgacgggg
    10861 atgcggggca ggtccgggtc gtattcccgg acgatctcga tatcctggaa atgaacgttc
    10921 cgctccaaaa tagccagggc attgtccagg acggcgttga tatcggccag gattttctcc
    10981 ggcacattct cccgggcgaa atcgaggatt ccccggacga ttttccggca gcgcagcgtc
    11041 tcgtcgcgga tgacctccag gtccttggcg tattccgtcc cctggagatc ctcgagaagg
    11101 aggctgctgt acgtgagcac gccggtcagg ggattgttga tctcatgggc gatgcctgag
    11161 gccagccgcc cgagcgaggc cagcttctcc gatttgagaa tcgtccgttc ggccatggcc
    11221 tggagctcgc ggtcgcgctc ctcgatggcc gcggccatca cgttgaatcc ctgggcgatt
    11281 ttccgcgtgt cgtcgtcgtc cttcgggagc gtctcgatcc ggtgatagtt gccgtcggcg
    11341 atgtcctggg tggcggccat gatccggcgg accgggcgcg tccagaggcc gaccaagtag
    11401 atcgagagga cgatcgcgat gagggtggtc aggccgatga ccaaaagata aaggacggcg
    11461 gtcgtccgga gaacgtcgtc gtacttgtcc ttgaggacgc cggcatagag catgcccacc
    11521 ggccggccgt cgatatcctt caggggggca taccccgaaa tgtaccagct gtcgacgacg
    11581 aaggccgggc cgatccaggt ccggccctcc tcgaagacgt tccggaagac ctcgtccgag
    11641 accagcgtgc cgatggcccg cttccccgcg ctgtccatga ccgtcgtggc cacccggaca
    11701 tcctcgagga aaatcgtcga ggatccgact tcgcggcccc ggttccgctc ctccttgaag
    11761 aggaggcggc ggaaccggtc gatgaagctg tcgttgttgt tgagcaggac ggccgcgtac
    11821 agaaggccga ccattcggcc gttctccatg atcggggaag cggccttcat gaccagggcc
    11881 cggtcttcga tgctcgactc gtgccggcgc gacagcggcg tcgggacggc cttgatgggg
    11941 gtccgctccg ccaggttgcg gccttcgttg cggatgttct cggggccgag aatgcccgtc
    12001 ccggcggccg gcttccggtt gcggagaacc cattggatgt gccggtaatg ggcgacgctg
    12061 tcgccccagg cgtcgaaatt gttggcccgg acgagaacgg tcccgtcggg attgaggacg
    12121 ttgacgatat cgaagccgaa ctccgtcttg atccggccca aggcctcccg tagataggcc
    12181 cggtcgccgg tcatcgtcgc ttggacgatc tcggaggtct tggccacgta ctggacgatc
    12241 cgggaccgct tctcgacctc ctcgttgtaa agctcggtcg tcgcggccag ggagttgcgg
    12301 accgactcgg tcgcctcgcg gacgaggttc ttgtcgatga tgttgacccc gatgaccagg
    12361 gagagcgagc ccgtcacggc cacgatcagc aggaggctga ccaccagctt gaaccggata
    12421 ttggaaggga acaagcggcg gcgcatggcc ggcggatcac ccgaggaggg ccgcgatttt
    12481 ctggacgagc tcgtcgaggt cgaccggctt ggtcaggaag gcgttgacgc cgggcaggaa
    12541 gctgtctccg ggatcgaggg agaaccggga gttggtgact tggccgatgg ccgtcaggat
    12601 gatgaggggg acggtccgga cgtcgcggta gcgggagaac ggccgcgcgc tccggatctg
    12661 cccggccgcc tcgaagccgg ccgtgtccgt ttccatcatc acgtccagga tgaccaagtc
    12721 cggcttttcg gccagcaggg tcttcagccc ctcggccgcg ctgtaggccg cggccgtctc
    12781 atagcctcgg gattggagga cctgggagag gctctcgacg aggtccttgt cgtcgtcgac
    12841 gatgaggatt ttcttggcca tgatcccccc ttaatccttg atcagcttgt agatgacgtt
    12901 gcgctcctcc caagccttgt tccggaggta cgcgtcgatc gggaattcca cctgtttgag
    12961 ctccacgggg gcggatttct gcgggacgag gcccagggcg agggccacgc cgtagatgat
    13021 cgcctccggg cggggcgggc atccggggat gagataatcg acggggacgg ccgtcgcccc
    13081 gccgccggtg acgttgtacg tgtcgaacca gcagccgccg ccggccgcgc acgacccgac
    13141 cccgaagacg agcttggggg cgggaatcgc ctcatacgcc cgcttgacca ggggaagggt
    13201 cggccgggtc accgccccgg acaggagcat gacgtcggcg tggcggggcg agccgacgag
    13261 cttgatcccg aaccgctcga cgtcgtaata cggcgtgagg acgttgagga tctcgatgtc
    13321 gcagccgttg cacgcgccgc tgttgcagtg atagagccac agggacttgg gaaaggcttt
    13381 cgccgccgcc cgggcaaaga gagccgccat cataaacctc cgatcttttc agggcgccgg
    13441 tacgcctcgg tctcggccag cgcctccggg gccatccggt ccgtcgtcct ccggaggacg
    13501 gtccgggcct cgaggctgtc atagcggtag ccccgaaggt ccagcttgtc gatcgcattc
    13561 ttgatttcca tgtcgaagca ccgcccgcac cggttgcagg tcagcatgaa cagctcgatc
    13621 gtctggctga catcctcccg ccggccggcg gccagctcga actccggcga catcacgatc
    13681 gccttttccg ggcagagatc ggcgcaccgt ccgcagtagg tgcaccgcga tccgtcatag
    13741 agaaggacgc ggatttcctg gcagaggtcc cgcaggagga tcgtccgggc cggacagtgg
    13801 ttggcgcacc cgccgcagcc gatgcacttc gtgtgatccc agaccggctg gccgcggaat
    13861 ccccggggga cgggtttctt gacgaagggg tatttcaggg tcaccgtccc gggactcagg
    13921 gccaggacga tttgcttgat cttgctggtc agccacatga ttcttcctct tctttcaccg
    13981 tccgagcact cctaaaccga cggccgcgag ggcggccacg atcaggacgg cataatagcg
    14041 gacggcctgg tcgatccgga gccggggatt cgtcgccccg accagggcga tgaggacgaa
    14101 gacgacggcc atggccagga gctggacgag gatgtcgagg gcgtagacgc cggtccgcag
    14161 gaacggcagg aacaccgaga cgaacagcca ggcgtaaaac atccgcttga tcattatcgc
    14221 atatttgaaa agggccaggt gagggccgga gtattcgatg aacggacctt ccagcagctc
    14281 cacctcggcc tcggcgatgt cgaacggctg gcgggcgacg aacgcctgca gagcgagcag
    14341 gtagacgacc agcatcaggg cggacgaaag tccgaaggcc gctccggccg ccgccggcgc
    14401 ggccgcggcc aggctcaggc tcttgatttt caccgccccg aggatcaggg tcatggccag
    14461 gaccggctcg accatgatca tcgtgatcat ctcccggctg gccccgatca ggccgaaggt
    14521 gttgcggccg gccagggcgc cgaacaggac cgaggcgccg gcgagggtca ggaggtaaat
    14581 gacgacggtc gcgtccatcc atccggccaa gacgcccgcc cgtccgccga aggggatcac
    14641 ggccacgacc gccagggtcg cggcgaaggc cagaacgggg gccagccgga acggccaggt
    14701 tccggcggcg tccaggcgtt ccttgcccag aagcttgaac aggtcgaaat agggctggat
    14761 gaccggaggg ccctgccgcg actggacctt ggccgtgagc ttccggagaa cgccgtcgaa
    14821 cagcggcgcg agcaggagga aaagaatcac gttgaggaac gggatgacgt agctcatgag
    14881 cgactccggg aaagacggtc catttctttt ttgctccaca cgcgtccgcg tcccgagcgg
    14941 cggtcgacga ccgcgacccg gtcggtgcag gagaagcacg ggtcgatgct cccgaggatg
    15001 atcgggaaat cggcgaactg gttgtcctgg agcatgcggg gaatgccctg gaggttggtg
    15061 tacgtcgggg cccggacccg ccaccgttcc ggccggttct cctcccccgt caggacgtaa
    15121 tgaaaggatt cgccccgcgg cgcctcgacc atcgaccagc cgggccggag ggcgggaagg
    15181 gcttcggccg cctcggtcag gacggggccg tcgggcatcc gctcgagggc ctggcggatg
    15241 agcttgatgg attcgaaaag ctcggccagg cggacgacga tcgtgcccca gacgtcgccg
    15301 tccggaacga ccgggacggc gaacgacagc tcgtcgtaag cggcatacgg atgatcgcgc
    15361 cgggcgtcga tgtcgacgcc gcggccccgg gccgtcggcc ccaaaaggct ccagagaacc
    15421 gcttcctcct tcggaacgtg cccgacgcct ttcgttctcc ggtggatcag cgtatccccg
    15481 acgatcgcct tttcgaagga gaccagctcg gcctcgaggc tcttgaggat ggccagcgtc
    15541 tccgtcttga tctcgggggt gatgtcccgg cgcacgccgc cgacgatcac catgccgtac
    15601 gtcttgcggt tgccggtgat tttctcggcc agccacatga cctgctcccg gatccgccag
    15661 gcgtgcatga agacggtgtc gtaaccgatg agatggccgg ccacgccgat gagcaacagg
    15721 tgggagtgga ggcgctcaag ctccaggcag acgacccgga tgaattccgc ccggcggctg
    15781 atcgccaggc cggcggccat ttccacggcc tggcaatagg cgacggaatg gacggagccg
    15841 cagatgccgc agatccgctc ggcgatgaag ggaacttcgt tgtagctgac ctgcgtctgg
    15901 cacagcttct cgatgccccg gtgggccatg aagccgcggt aatcgcaatg cttgatcgtc
    15961 tcgccgtcga catagacggc gaaatgggcc ggctcgtgga gggtcgcgtg gaacgggccg
    16021 accggaacga tcgtcgtccc gggcggcgcc tcgtcgagct ggtaggcgac gtcctcggcc
    16081 gcgggaggga tgaggttgta cggaacctcc cgccggagag ggtaaatccc gtccggccag
    16141 tcgtcggccg tgacgagctt tttcggcttc gggtggcccg ggaaattcat tcccaaaaga
    16201 tcgcggcatt cccgctccga ccagccggcg gccggaatgt ccggagtgat cgagtcgaag
    16261 tccggatgat cggccggcgc ggccgtccgc agcccgacgg tgaaatggtc gccgtcgaag
    16321 gcgaagaaat ggatcaggcc caacgtcccg ttccgatgac ggtcgtcggt gccgaccccg
    16381 acctggtagc ggccgccggc tccgacgagc cggttggccg cgtcccggag ccgggacggg
    16441 tcgatgtcga taaacaggcg gccgggcgcg gccgggctcg atccgccgac ggcgccgccg
    16501 aattccgcct tgagaacttc gaagattttt tctggagtca tgactcacct ccgccggtcc
    16561 agcggaagaa atgtttcagg gcggcgaaca tcccgcggtc gacgtaccgg ttcttcccgt
    16621 cgagctgctg gtagccgcac agccaggtgg ggacggtccg cgtcttcgat cccgccgagc
    16681 ggcgcaggag cgcggccgcc aggatcgcga cgacaagcag ccccaggata agaaggggcg
    16741 tcagcgcgct gaaatgcccg gcggcggcct cgccgattcc cgggacggcc gccaggccgg
    16801 ccgcccgctc cgcggccgac gacaccccgc ccaggaacaa ggacggggcc aggccctgga
    16861 gaatcaccag gattcccagg atgatttccg gcagcagcat cggaagcggg acctcgcgga
    16921 tttgtttccg ggccgtttcc tccgcccccg aggcggtgaa agccaggccg aaaaacttga
    16981 cgtagcaggc cagggtgacg gcgctggtga acagggcgat gattccgaac aggacgagaa
    17041 cgggggaaac cgcgccggag agaagggcgg ccgagatgat cgtttccttg gaagcgaagc
    17101 cgctggtggg cggcatcccg gaaatcgaca gcgaggcgat cgcggcgatg acggcgctcg
    17161 cgggcatgag cgtgatcagg ccgccgagct tgttcaggtc cttcgtcccg gccgcgtaat
    17221 gcaggttgcc ggaaatcagg aagaggaggc ccttgacggc ggcgtggttg acagcatgga
    17281 agagagcgcc gagaagggcg accgcggcca gggcccggag ggccgggtcg gaggcggtcg
    17341 aggcccagag agacacgccc agggccagga cgatgtagcc gacttggccg atcgagctga
    17401 aggccagcag ccgcttggcg tcgctctgct tgaccgactg gagcgtcccg acaaacaggg
    17461 tcaagacgcc gatcgcggcc agggcggggc cccagaagca gggattgaac gcggccgcgg
    17521 ccggagccag gcagaaaaac acccggaaca ggccgaagac gccgatcttg agcatcgccc
    17581 ccgacaacaa ggcgttggcc ggcgccggag cggccgcctg ggcgtcgggc agccagatcc
    17641 ggccgagggg aaagacgccc gccaggaatc caaagcccag aacgaacagg gtcataacga
    17701 tgacaaagcc ggctccgtcc atcgcctgga ctttttggcc gagagcctgg agggattccc
    17761 ccaagcttcc gccggccgcg aggcctccgg cgatcaccag gagccaggcg gcttccatca
    17821 gaacgagaaa cttgccggcg gcccggacgg aggccgcgtt tttctcgaaa cggatcagga
    17881 aaaaggacgc gacggccatg acctgccagg cgatggtgaa tccggccgag aggtcgttga
    17941 cgacgaccag gccggccatc cccaggacga acaggggcag ggcggcgtaa aatccgccca
    18001 ggcggcgttc ggccggaaaa cggtccgcgt accgcatggc atagatcgcg ctcatggccg
    18061 ccaggaagga cacgatgacg agaaacaagc cggccaggcc gtccaagagg atcggaaccg
    18121 cgatcgggcc gagggaaacg gtccgggccg cggccatgcc gcggccgaaa acagccccgg
    18181 cgccgaggac caggaagacg gccgcggccg cggcggtcag aaccaggtga acgattccgg
    18241 ccgcccgacg ccgccgggac aacagggggg caaccagggc ggcgagaagg agaaggccga
    18301 aggcggccca aagagccgga gaaatcgggg tgctgagcgt ggacatcaga tcgtctcccc
    18361 cagggttttg atttgccgat acgtatagac cgggccgtcg gtgcagacca gggtccggcc
    18421 gatcgcgcaa tgctggcact tgccgatccc gcatttcatg tggcgctcca gcgtcgagat
    18481 gatgcattcg tccttgaggc cgcggcggga cagctccccg atcacggcgt tgaacatgac
    18541 cggagggccg cagacgaagg ccgtcgtgcc gtccaccggc accttggctt tttcaatcag
    18601 ggtatggacg aacccgacct cgcccgtcca gccgggctcc tcccggtcga tcgtgacata
    18661 cgtctcgaat ccctccgtcc tccgccattc gtcgatgagc ggacggtaca tcagctcttt
    18721 cgacgacttg gcgccgtaga gaaggatgat ccggccgaac ttctcccggt ggaggagggc
    18781 atgaaagatc gaggaccgga gcgggatcag gccgatgccg ccggccacat agatgaggtt
    18841 ccggcccgcc atctcctcga acggaaagcc gttgccgaac gggccgcgga ggccgatctt
    18901 gtccccgacg cccagggcgt gaagggcgga ggtgaccttg ccgacgttcc ggacggaaag
    18961 atgacggcgg tccttctccg ggctccaggg cagggaaacg gcgaattcgc cggccccgaa
    19021 cacggtgcac atcacgaact ggcccgaacg gatggaaaag gagcgctcga gctccgggtc
    19081 ctcgaagcgg aaatagaagg ttttgacgtc ctcgatctcg tcccggatct ccgtcaagac
    19141 ggccggaacc ggaacggcga ccgcggcggc gttttttctc ttgtccatgg cttacttccc
    19201 cgtgatctcg cgccggatcg cggtgacgac attggccatc ccgatgtcgc cggggcagac
    19261 ccggacgcag cggccgcagc cgacacagcc cggccggagg tattttttcg attgagaaaa
    19321 cgaaagcttg cagaagaatc tcttgttccg ccggtcctcg acgggccggc gggggttgtg
    19381 ctccccggcc atccgggcgt agccctcgaa cgagcaggaa tcccaggtcc ggagccgctc
    19441 ggtcgtcccg tcccggcggt tgacatcctc gatgttgaag caggaacagg acggacagac
    19501 gaacgagcag gccccgcact cgaagcactg ctggccgatg tattcccagg tctcgttctt
    19561 gacgaaaccg gtcgtcaggc ggttgaccgc ccgggatatc caagccttgt tctcgaccgc
    19621 cgccgcctcg aaccgcgaga tcgagcggtc gatgacctcg tcccgccgcc ggccggcgtc
    19681 cggcccggcc ggaggcaggc tcagggacgc ggccagctcg cggccggcgt cgctcccggc
    19741 ctcgaacagg taagcctcgc cgaggtcggt caggttgacg tcatatccct cgcgggccgc
    19801 cggaccgctg tcggtgcaga cgcagaaaca ctgggcgaag ggggtgacgc acgtgacgga
    19861 gacgagaaac atccgccgcc ggtgggcccg gtagaattca tcgacgaatt cctggccgag
    19921 gaagaagcgg tcgaagcaaa gaagacccgt caggtcgcag gaccggatcc cgacgagggc
    19981 cttcggccgg acgagatcct ccggaacgat ccgaagatcc ttggtttccc ggtcataggc
    20041 ataagaaagg atgcgctccc agtgggggaa gaccgcgaac ttgggagaat tggccgggat
    20101 cggggcgtcc agccggatcg acgaggaatc ggccgcctta tcgaaaaaca cgtccccgga
    20161 aaccggatgg acctgggggc cgtacatctc gaagcgggcg gacagggcgg agagaaagcg
    20221 gggaacgtcg gatttggcca gaagatacat ggtcacacct tttccaggcg gacggggatg
    20281 acggcatcct cgccgtattc gaccgcgtcc gcgtgaggca cgagaaaatc cgggatcatc
    20341 gaacccacga aatagggcgc gtacaccgtc ccgggcggca cttcttccga gacccgggcc
    20401 agcggagtca tggatccgcg ggcggagacg acccggacgg gacgcttgtc ccgaaggccc
    20461 agggcggccg cgtcgtcggg atgaacctcc accaagcccc ggggatagag aagaaggagg
    20521 gcgttgtatt cccgccgggg gatgaaggtg tttttcatga tgttgttcaa gtgccaatag
    20581 aaataagacc ggccggccat caggcggaag gggaattcgg ccggggccgg ctcggcggcg
    20641 gcggaaaagg cgccgttgag ggggacgaag cgaaggcggc tttggtccgg tccggatccg
    20701 cccggccact gccatccgcc gaaccgggat tccagggccg ggtgggccgc cccgtgatat
    20761 tccggaatca tccgggtgat ctcctcctgg acgtcggccg cggaccggta gggccaggcc
    20821 tgtccccgcc gggccgccaa atcggcgtaa atccgccagg gcgggcgggc ctcgaaccgg
    20881 ggctcaatct tgcggcggtt gagctggatt ctccgctccg tgttggtgta cgttccgtcc
    20941 gtctcggcgt aggtcgtcgt cgggaggacg acatgggccg tttccttgaa cgggttggcg
    21001 tacgctccga ggtagagaac gaactcgagc gacttgatcc ggccggccag aagctcgatc
    21061 tcgggatcat ggtcgacgac caccagggcc ttgagtccgg aggaagcgtc ggccaggagg
    21121 ctttcgaccg aacggccgcg cccggcgttg acgtccttcc cccagctccg ggcgaaggac
    21181 gaggccgcgg cctcgtccgg aagccgacgc cagcccggca gaacgtccgg ggcggcgccc
    21241 atgtcgttga ccccggccag gttggaaacg ccggtcacgg gaaggatcgc gcatccctcc
    21301 cggccggcct ttccggcggc caagaacagg ttgaaaatga gggccgtggt ttcccgggtc
    21361 aatccggtga tcccggtcga aaagatgatg acggccctcc gggcggaagc caggcggcgg
    21421 gcgacgtccc ggatcttgtc gaacggaagg ccggtccggg cctcgaggcc ggcggattcg
    21481 agaccctcca gggatttgcg gaatccgtca aagcccgccg gcgctcccga cgccgaatcc
    21541 ggcggatggt cttcgaggag ggccttggcc atggcggcca tgacgatgtt tttcgttccc
    21601 ggcttgttct gcaggtgcgt cccgctgagc ttggccatct gggtgaggcg cgtgctgagc
    21661 gtgacgacgt ccgccccccc gcgggcggcg aaatgaacgt cgctggcgat gatcggattc
    21721 tgccgggtca ggtcggtgcc gacgacgaag atgaagtccg ccttccgaat gtcggcgatc
    21781 gaacccaggg cgcccggccg gccggttcct tcccttaaaa cttcggccgt cgtcggatac
    21841 cagacgtcgg acaagacgct gatgttggcc gagcggaaga ccccgcgggc cagccggacc
    21901 agggcgtagg cgtcctcgtt ggaggatcgg ggcgaagccc agaagccgat ctcctctccc
    21961 gaaaacgccc ccaggcgggc ggcggcttcg tcgagcgcct catcccaaga aacgggagtc
    22021 agccctccgc ctttgcggac aagagggcgg ggaatcctct cctcggtgtt gagaagctcg
    22081 tggatgtgcc agccccggcc gcacagccgg ccccgcccga cgggatggga ggacgagggg
    22141 aacaccccgc ggatcgtccc ttcctccacc tggagcatgt ggccgcatcc tgttccgcag
    22201 aaattgcata tcccggtttg atagctcatg accgctcctc gtcctccctc ctcgggggaa
    22261 ggacgttgat cgatgttccg tccccggcgg tccgggcggc cgaaatcgag tcttcaagac
    22321 acttcggccc gacagaaggt tctaggaatt ggataagagg aaaatcctag gtgccaatct
    22381 agcaaatctc gtgaagaaag taaagggggt gtttgacaga accggccgaa gcggataaag
    22441 tgaattttct aaaggagaaa aaacctcgtc cgatctcatt cccgtccgga gaacgccatg
    22501 aaaaaatcat tccgcgcctt ggcgctcggg cctgttttgc tctgcctcgc cgcttcggcg
    22561 gcgtccggcg ccgtgggccg gcccggtgat tttcagttcg gccacttgac gattttcaac
    22621 ggcttgtccc aaggctcggc catggccatg gtccaggacg aacgggggtt tctctggatc
    22681 gggacggagg atgggttaaa ccgctacgat gggtacggct ttaagatcta tcgtccgacg
    22741 gcggggccga acggtctctc cgacagcctc atcaacactc tattcctgga ctccaagaag
    22801 acgctgtggg tcgggacgat gaggggcctg aaccggtatg accgggaccg cgacgacttc
    22861 acccagtttc atccgcatcc ggccaatccg cggagtctga gccacgacta catcacggcc
    22921 ctcgccgagg acgccgatgg aatcctctgg atcggaacag ccgggggagg attgaattcc
    22981 ttcgatccgg cgtccggcga attccgggcc ttcctcagcc gaagggccga acccaaaact
    23041 ctcagccatg acgaaatcct ctccctctgc cgcgacgccg cgaattactt gtgggtgggc
    23101 acggccgacg gattgaaccg cttcaatccg cgctccggcg aatccgtccg cttcaaccat
    23161 gatccgggca acccccacag cttgagccac aaccgcgtcc tggccctcta ccaggacggc
    23221 aacgggatgc tctgggtggg aacggccaac ggcctcaacc ggttcgatcc ggagaccggg
    23281 cgtttctccc gttatttcaa ttccacccgg gatccccgga gcttgagcga caattacgtg
    23341 acagccgtct tccgcgaccg cctgaaacgg ctctgggtcg cgacccagaa cggcctcaac
    23401 ctgtacgatg aggcggccga cgatttcttc gttctccgca acgatccgac ggatcccaag
    23461 agcctggcct tcgacgacgt ctacagctta ttcgaggacc ggacgggcgt cctttgggtg
    23521 ggaacccgcg gccgcggcat cgacaagctg gccgcggaaa attcccgctt cgccctgtac
    23581 cagccccggc gcggcaatcc ccggagcctg ggatcgggtt acgtccgggc catcgccgaa
    23641 agccccgacg gcggcgttct gtatgtcggg ctccaggacc ggggcgtgga cgccatcgac
    23701 cgggccacgg gcgaatacac ccatctccgc cgcgtcgagg gcgtcgacaa ctcgctcaat
    23761 tccgagctcg tctacgccct cgccgtcgac gattcgggaa ccctttggat cggtacctac
    23821 ggcggcggat tgaattccta tgacccgcgg accaagacgt tccggcatta cgttcacgat
    23881 ccgggcgatc cgcaaagcct cagctataac gacgtccggt gtctggccgt cgacggtccg
    23941 gatcggcttt gggtcgggac ggacggcggc ggcttgtgcc tgctcgacct ccgcgcccgg
    24001 aggtttatcc gctaccggaa cgatcccggc gacccggcaa gcttaagcca caaccaggtc
    24061 cgctcccttc acctcgatca cgccggaacc ctgtgggtcg ggacgttcgg cggcgggttg
    24121 aaccgcttca accgggaaac cggcggcttc gccgtctggc ggcacgaccc ggccaaggcc
    24181 tcggccaccc tctgcggcga tttcatcccc aacatggccg aggattcccg gggacggctg
    24241 tggatcggga cgacggccgg cttgaatctc ttcaatccgg ccgacgggac gttcgagtgc
    24301 tacaccgagg agaagaacgg tcttccgaac aacgcgatct acggcgtcct cgtcgacgag
    24361 gaggactcgg tctgggtttc cagcaaccgg ggcttggccc gatggaaccc ccaaacgagg
    24421 gctctgaaaa catacgacat ctccgacgga ctccaaagca atgagttcaa cggcggagcg
    24481 aggtaccggg caaaatcggg agaaatgttc ttcggcggaa ccagcgggct cagcgccttc
    24541 ttcccggcca acctcaagga caatcctttc cggcccgagg tcgtcatcac caatttcgag
    24601 gtcttcaaca agccggtccc cgtcggcggc gaggtgaacg gccggatcct cctcgataaa
    24661 tcgatcactg aaacgaccga tatcgacctc cggttccgcg accgggtcat caccttcgag
    24721 ttcgccgccc ttcattacgt cgcgcctgaa aagaaccgtt acgcctacat catggagggc
    24781 ctcgacaccg gctggcagga agtccagggc cgccggttcg tgagctatat gaatctcgat
    24841 cccggccgtt acgtcttccg ggtgaaagcc tccaacaacg acggcgtttg gaacgaagag
    24901 gggacggcgg tccatctccg ggtcattccg ccgttctata tgatccgctg gattcaagcg
    24961 ctggccgtcc tgctggcggg tttggcgatc tatggggcgg tccgcaagcg catccggaac
    25021 attcaccgtc ggacgatcga gctggaggag aaagtccagg aacggacggc cgaattgaaa
    25081 aaggccgagg gcgaggcccg cgaggccaac cgggccaaaa gcgagttcct ggccaacatg
    25141 agtcacgaga tccgaacgcc gatgaacggc attttcggca tgaccgagct ggctctcgaa
    25201 accgatctct cccccgacca gcgcgagtac atggaagcgg tcaaggcatc ggccgattcc
    25261 ctcatgacga tcatcaacga cattctcgac ttctcgaaaa tcgaggccca aaagatcgag
    25321 atggagaaaa tccctttcca cctccgggac acggtccacg cggccgtgac cggcgtcgcc
    25381 ctcctggccg aacggaagag cctcgagctg gcctattcga tcccgggcac cgtccccgac
    25441 gggctgatcg gcgatccggg ccggttccgg cagatcctga ccaacctctt gagcaacgcc
    25501 atcaagttca cgaacgaggg cgaagtcgtc gtttccatcg acgcggcccg gcgggccgat
    25561 gatcaagtct ggctccgcgt ccaggtcaag gacaccggca tcggaatccc gcccgacaag
    25621 cagcgcctca ttttcgaggc cttctcccaa gcggacacga ccaccacccg aatgttcggc
    25681 gggaccgggc tgggcttggc gatttccacc cagctcgtcg ccctgatgaa gggaaagctc
    25741 gaggtcgaaa gcgagatcgg ccggggcagc acgttcttct tcaccgttcc tttcggcctt
    25801 catcacgagg aagggaaggc ggtcacgccg ctccgtcttt cggacctgaa ggacctgccc
    25861 gtcctcgtcg tggacgacaa cgccacgaac cggaggatcc tccacgaaat gctgtcccac
    25921 tgggggatga ggccggtgac cgtcgacggc gcccaggccg ccctcctggc gctccagcag
    25981 gcccgcctcg agggacgccc gttccgcttg atgctgaccg acgcgaacat gcccgagatg
    26041 gacggcttcg acctggccgc ccggatcaag gaagagcccc attacaagga catgctcatc
    26101 atgctgctga gctcctcggg attccggggc gattccagcc gctgccggga tctcggcctg
    26161 gccgcatatt taacaaaacc ggtcaagcag tcgcttctcc tcgacgcggt gatgatgtcg
    26221 ctgggcgaca aggaggctcc cgccggccaa gcccggttga tcacccgcca ctccatcgcc
    26281 cagaaccgtt cccgttattc cgtcctcctg gccgaggaca acatcatcaa tcaaaagctg
    26341 gccgtcaaaa tccttgaaaa ccgaggccac cgggtgacgg tcgtcgaaaa cggcgaggag
    26401 gtgctggacg ccctggagaa gggctcgttc gatctcatcc tgatggatgt ccagatgccg
    26461 atgatggacg gcttccaggc cacccgggaa atccggcggc gggaggtcgc cacgaaggcc
    26521 caccagccga tcgtcgccat gaccgcccac gccatggtcg gggaccggga gaaatgcttg
    26581 gaggcgggga tggacggcta tatctccaag cctctcaagc cgctcgatct tctccggacg
    26641 atcgacgaaa ttctggcgcg tcgtccgcgc gaggaagaag aggaaccggt tcttccggtc
    26701 gaggaggaca acgaggagcc gcgtccctcc tgaggatacc gggacgtgat tcccgggccg
    26761 gcggacacca aggaaaagga acggccgccg gccggacgaa tcaccgcccc gccagcgcgc
    26821 ggtaaagacg ctcgggagag gcgccgaagg atgcggccag atcctccaag ctccgggacg
    26881 gatcgtcgac ccgccaaccg gcccgggcca ggcgttccat ggcgtcctcc acggacacgg
    26941 ggacgctccg gcaaagctcg gccagactca tcccggcggc ctcggccgac gggggggaaa
    27001 ccgtgacggc cagggcgccc tgcttgatcg ccgcccgggc gtccatcagc ctccacagcg
    27061 gcttccaccg gaggaccgcc ccggccgtca ggacgacgac gatgagcgtc gcggccaggc
    27121 attcggtttt cgccccggaa agagccgaag ccctccggcg gagatagccc caaagagctc
    27181 tccaattcag gatgacatgc aggacggcga acgccatcaa ggcgacgacg atcagcgtat
    27241 gcaccccttc ccagctcttt ttatcgagac cgagaacgga ccagccgctc caggcggcca
    27301 cgcttccttc cggacggaaa tacatgacga tcccggaaac ggcggccgcc aggaaagacc
    27361 agaggagtcc aaaagaggcg aacgcccggc tgcggaagcg ttttccggct ggtttcgaag
    27421 cggttgcggt catgattttc ctcctccgga ctgtcctgaa tccagcccgg gcgttcgttt
    27481 atcgagatgc aggcggatta aatagagaac ggtgcacaga tggctgatcc ctccgatgaa
    27541 ccgttccttg accggccctt tgggaattcc ttcgatcgat tcccggattt cggccatcgc
    27601 cgcctcgacg gcggccaagc cggcctgaag ctcgtcatcc gaaaagcggc cggagagaac
    27661 cccgacccgg cccggattct gaaagactcc gcgataaaat tcccgctccg ccggggacaa
    27721 ccgccggatc agacgctccg tgacgcagag ccggacgagg gcgtatcccg ccaggacgga
    27781 ttcgcggtcc cggttgcagc ttgtgtcggg atgccgccgg aaataggctt cccccagctc
    27841 gatcatttcc gcggcgtcga ggacgaaatc gccgccgagc gcctcgacgg catgccggaa
    27901 ggcttccgca tcggggcaac gactgtataa ctctccgagc atcgggttag agggaaagcc
    27961 cccgccccgt aaggacgggc tcttcagctc ttggcgatcc gccccatgac ggccaggagc
    28021 aggtcgaaat tgaccggttt ctggaagatg ccgctgaagc cgagatggcc gaatcccacg
    28081 gtttcggccg tcgcgtctcc gatcgagctg aggaggaaga tggggacgtc cggccgctcc
    28141 ttgcgcatga tcttggcggc ttgggccccg gcgtccaggt cctccatcat catgtcgcac
    28201 agcaccacgt ccggacggct gcggcggaag gcggccacgc cgtccttgcc gctgtaggcg
    28261 acctcggttt gaaaaccgac cgcttcgaga accgctttca ccgaatcaca gatcgccgga
    28321 tcgtcgtcga tgatgagcac ttttttcgga tcggacatgg ccacctccga gaaacggacg
    28381 tcgggcttat tctactcgat cgaccggccg aaatccagac cgccgatgga cggaggaaag
    28441 gcggttcagt tacaataccc gcgccggcga cggacattcc tcaaggacgg aactcatgaa
    28501 cacgggaaga aggatttctc gggcggaacg gctctggggc ggtcttctcg ccgcggctct
    28561 cctggccggg acggcggccg cttcccaaac gggcgagatc cggggcaagg tcctcgatga
    28621 aagcggcctc ggccttcccg gcgtggaaat ccgggcggcg agcccggccc tccaaggcgc
    28681 ccggaccgtt ttttcgggac gggacggctc gtttcatttc cccctcctcc ccgtcgggac
    28741 gtattccctg accttcaagc tcgccggctt caccacgatc gtccaggagg cggccatcgt
    28801 ccggctcggc ttgacgacga ccctgaccgt ccggatgccc caggcggcct tggaaacgga
    28861 agtcgtcgtc acggccgaaa ccccgctgat cgaccggtcc tccaccgaca cctccacccg
    28921 gctgggggcg gccgacctgg aaaaaatccc ggcccagaac cggacggtcg tggacgtcgt
    28981 caagttcacg cccggggcgg ccggcatccg ggccgacacg cggcggggca cgtcgaccga
    29041 gggtcagccc agcttccgcg gggcgggggc ggagggcaac aactgggtca tcgacggcct
    29101 ggccgtcagc ggagtccgga tgaaggattc cggggtcaat cttaattacg attcgctcga
    29161 ggagatccag atcatctccg atccgttctc ccccgagttc ggctcgtcct acggcggcat
    29221 catcaacatg gtcacgaaaa gcgggggaaa ccggttccac ggcgaagcca gcctgctgat
    29281 cgaaaacagg catcttcagg cggcccgcca atcccagctt tcggtcgtca gcgagccgga
    29341 cgccttcaac aacacgagcg cttatgtcaa tctcggcggt ccgatcctca aggacaagct
    29401 ttggtttttt ctttccaacg attaccagtc gctgaccgag gagacgaagg aggcggcctt
    29461 cggctacctg gccgttccgg gcggacggag aacgatcggc cgcgacaaca tcttcgtcaa
    29521 gctgagcttc gcccctcacg ccgatcacgg cctttccctg acgttcctcc gggaccagtc
    29581 gtttccccag aggggaggga tcgggcttcc cgagatgaac gaggaaaagc gggtccggga
    29641 cgccatgttc cgcctcaact accggggcat cctcagcccc cggacgtttg tcgaggccgg
    29701 cctgggccgg gtccggcggg acctggacac gacgccggtc gacggcgatc tcgggccggc
    29761 gatgtactac gtcaaggacc tggcccgcaa cgtccataat tcctacggca acgtgatcga
    29821 cgacgagcgg cggttcgacg cccaggtgaa gatgaccacc tactttgaaa cgccgcggtt
    29881 cggccgccac gagatcacgg ccgggttcga gtactacgac gtgtcttcgg atttcgaggt
    29941 ggatttcagc ggaaagaacg aggatctctt ccccggcaac ggcttcgatg tcggcacgaa
    30001 atacatcttc gaaacccgga agggcggcgt cggggttccg tccggcttct acgagtacgg
    30061 gccgttcgat ttaatcaacg cctcgagggg gatcggactt tttctgaagg acaaggtttc
    30121 ctgggaccgc ttcaccctca tggccggcct ccgcgctcaa acccaagcct gcttatccga
    30181 tacgggggag acgctctgga gctggggcct cggtgatttt ctctctccgc gggtgtcctt
    30241 cacggccgat ctgaccaagg acggccggaa cgtcctcaag ctggcctggg ggcggttcag
    30301 cgacatgatc acgacgatgg ccctcggctt cttcaattcc ggaatggttc cgacctatcg
    30361 tctctaccgg tgggccgggc cggagtcgcc cggcgaggcc gacgtccaca atcccgactt
    30421 ctgggccttc gaaatccagc agagccaaaa attcgacatc aatccgcgcc tcaagcccga
    30481 tttccaaacg cgctggcttg tcgaattcga ccgccggatc gggccgtcct gggcggtccg
    30541 ggcccgtttc gtccggacca cggccgacaa cctcctcgag ctcctgatga tcttcgatcc
    30601 gcggacgatc taccgtttcc tctacgacaa cttcgaatac aagcgccggg attacaccgg
    30661 attggagctg gaaatcaccg ggtcgatcgg ccggcgcctg ttcctcaacg cctcctatgc
    30721 ccgtgccctg gcccggggca cgaatcccgg acaatcggag accggccgat ggtccgagga
    30781 ggaaggcagc acgtattacc tggggatgtt cggaaaccat atctacgtcc ccgacatgcc
    30841 cgagctggcc gccctgaagg cctgggccga tgccgccctg gccgggctgg gcggccggaa
    30901 catcggcgat gagggctggt acgggcgtct cccctattcc gtggaccacg acgtcaagct
    30961 caacgccgtt tacagcgccc cggccggcat ccaggtcgcc gcggccttcg aattcatttc
    31021 cggctacccc tgggaaaaaa caggctacgt cccgtttttc ggaggatatt acgccttccc
    31081 ggaaggccgg ggcgcgcgga ccacgcccgc ccattccttc ctggacgttt cgatcgagaa
    31141 atccttcgcc ctgccgggct cggggatctt cgccgacgcg gccgtggccg tccgcctgga
    31201 cgtcttcaac gtcttcaaca gccagcggcc cgtttcctac gtcaaggagg atacgccgct
    31261 gttcggcgac gtttggggac gccagcagcc gcgccaagcg cgcttgtcgg cgaaaatcaa
    31321 gttttaagct ttattcgacg gtcacggact tggccaggtt ccggggctgg tcgacgtccg
    31381 ctccccgctt ggccgcgatc tggtaagcga acagttgaag gggaacgacc gccaggaacg
    31441 gggtgaggag agggtgggtc gccggcaagg ggatcaccgc gtccacgtgc tcccggaggg
    31501 ccgcgtcgtt ttcatcggcc gccgcgatga caaagcccgc ccgggccttg atctcggcga
    31561 tgttgctccg cattttgtcg tagacccggt cgcgggggat caaagccatc gtcggcatct
    31621 tctcgtcgat caaggcgatc ggtccatgct tcatctcccc gccggcgtag gcttcggcat
    31681 gaatgtagga gatttccttg agcttgaggg cgccttccat ggccacgggg taattcaccc
    31741 agcgcccgag atagagaaaa tgggaaaaag ccatgaattg ctgggccagg tcttcgatcg
    31801 ccccggcccg atggaggatc cgctcgacct tgtgggggat gcgctggagc tccgcgatca
    31861 ccgcgagccg gtcctctttc gttcccgccc ccttcgcctg cgccaggccg agggcgacca
    31921 gggccagggc ggtcagctgg gcggtgaacg tcttggtcgc ggccaccccg atttccgggc
    31981 cggcgtgggt atacagaacg ccccgggatt cccgggcgat cgatgagctg acggcgttgg
    32041 tgacggccag agtcgcggcc gccctccctt tcgccgcccg gagggcggcc agggtatcgg
    32101 cggtttcgcc cgactgggaa atgacgatga ccagggcatc cttgtcgaga atgaaatccc
    32161 ggtatcggta ttccgaggcg tattcgacgt cggccgggat gccggccaga gattccagga
    32221 agtatttccc gatcaagccg gcgtgggagg atgttccaca ggcgatgatg accgccttcc
    32281 ggacatcccg gaaaacctcg gccgggatcc cggtctcggc cagcaggacc tcgccccggt
    32341 cgagggaaat ccggcccatg agggtgtccc ggataacctg gggctgctcg tagatctcct
    32401 tgagcatgaa atgcttgaaa ccctgctttt cgaccatgag gggactccag gagatcctct
    32461 cgattttctt ttcaaccggc gcgcccttga aatccaggaa acgggggccg gaggcatcga
    32521 tgacggccat ctcccggtcc tcgaggaagg ccacccggga ggtatgggcc aggatcggat
    32581 tgatgtccga ggagacgatc gtctcgccgt ctcccaagcc gatgaccagc ggggggccca
    32641 tcttcgccgc gacgatccgg ccgggatcct tgaccgcgat ggctccgacg gcgaaggcgc
    32701 cctccatctc ggccagggcc gcccggacgg cctcctccaa cgatccggag aaatgcttct
    32761 cgaccaggtg ggcgatgact tcggtgtcgg tctccgtccg gaagacatga ccctcggcct
    32821 tcagctttgt cttcagggcg agataattct cgatgatgcc gttgtggacg acgacgatcc
    32881 cgcccgtgca gtcgacgtgg ggatgagcgt tggcttcgct tgggcaccca tgggtcgccc
    32941 accgggtatg accgaggccg taggcgccct cgatcggcgt gcggcccagg cactcctcca
    33001 gagcggcgac cttgccccgg acccggcaaa ggcggatgcc tccaccgccg gcgacggcca
    33061 ctccggccga gtcgtatccg cggtattcaa gctttttcaa gccctcgagc aaaacgggga
    33121 cgacgtcttt cgggccgata tacccgacga ttccgcacat gcgaaccccc ttcctcgggg
    33181 cggatcatcc cccgcgcggg acggccgcgc taggacttcg atccgccgtt tttccccgcc
    33241 tgaagcttcg ccctccggac cgccgcccat ccgggcttct cgacctgccg gccccgggcg
    33301 acggccaggg cgtcgtcggc gacgtccttg gtgatgaccg agccggccgc gacgtaggcg
    33361 ccccgcccga tgcggacggg agcgatgagc tcgctgcccg agccgatgaa agccttggcc
    33421 ccgatgacgg tccgattctt gcggacgccg tcgtaattgc aggtgatcgt cccggcgccg
    33481 atgttggccg ccgcgccgac ttcgctgtcg ccgagatagg acaaatgcat ggccttggag
    33541 cccggaccga aatccgtctt tttcatctcg acgaaattgc cgacccggga tccggctcga
    33601 agcgaggtct gcatccggat ccgggcgaac gggccgattc ggacgtcgtc ggcgacggtc
    33661 gcatcctcaa tctcgctgga gccgaggaca tggacattgt cgccgaggac ggatcggacg
    33721 atgcgggcat ggggatggat ccggctgttg cgtccgatga tcgtcggccc ctcgatgacc
    33781 gccccgggat aaatcaccgt ctcggccccg acgcggacgt tccaatcgat ccaggtggtc
    33841 gccgggctca aaatcgtcac tccgtgttcc gccaggtccc ggatcttgcg caggcggagc
    33901 cggtcggccg cttgtccaag gtcgaaccgc gtctggatcc gacggacatc ctcgggctcg
    33961 ggagcaagaa cgacgccgac cggatttccg acggcggcca gaaggccgaa ggcggcggtc
    34021 aggtcggatc cacctttccg gcccgtccgg gcggcccggg gcagaacccg gtcgagcgcg
    34081 ccggcctgga agacaaacac gcctgtcggg acgcggcgcg gaatcgcccc ccggtcctct
    34141 agaatcggaa tccgcgcgct tgagggcgga atttcggcgg actcggccca taacatcgtg
    34201 ccggcgtatt tcttccggcg gtgccgggaa acgagattcc ggagaacggc gggggggatc
    34261 agggggaggt cggcggggag aatcaagacg tcggccgcgg cgtttccggc ccattccttc
    34321 cgggccgatt gcacgatcgc ggccggattg ccggccttcg ccttggccgc gacgacctcg
    34381 gcggacagtc cgggaatccc ggtcttgacg gcttcgggat cgagtccggc gacgaccaac
    34441 cgcttggccc cggccccggc ggcggccgcg gccgcccatt cgagaagggt cattcccagg
    34501 agggaatgaa gcgctttcgg agtcgtggac ttgaatccgc cttcgtcttc ccctcccgct
    34561 gaaatcaacc cgatcaggag gttatccatg aatctttggg gcctgtctca agacgtcttg
    34621 gtgatggcct tgaaccgttt gtcttcccag aggggagcga agtcgacttc gttttgggcc
    34681 aggatccggt aggctttttc gccggccacg gcctggcgaa gggcctcgag ggattcgtcg
    34741 agccggccgc gccggcacag gagatcggcc tgaaggtaga ggaggcgggc gtcggccgga
    34801 tcggacttca gggctttttc gacaagcttc aaggcctcct cggcggcccc ggtattcatt
    34861 ttatagaccg cggcgctgac gtaatcctcg gcggtcttga gcgctggaat ttcccggggt
    34921 tccttgaagc gctcggcggc gatcgattga taggtccgcg cccgggcgac taggtcgcgt
    34981 tcagccggga atttctcgat gaatgccccg aatgattcct gggctttgtc ccacttcgat
    35041 ttacggaact ccttcatggc gtcgccgtag gcggtcaaag ctttttggta ttcgtccttt
    35101 ttactttttt cttctttcaa agggttctct ccggatccaa gatggcgtgt attttatcca
    35161 agatcaagcg gaaagacaag agatcccccg ctcttcatga tgggattcgg ccgcgagaag
    35221 acgggaaaag ccgttcgttt tcgttacatc gcggcggagg aaaactcgct ttgcggccga
    35281 cgcccgccgc ctcgccggcg tcgagacgga agacaattcc ggaaaacgtc cggcgtttga
    35341 ttattttcgg acagcggggt ttcggcatcc gctattcccc caagaccgtc aattccggat
    35401 gatcgagaag atgcctgacg gcggcgaaat ggaggcggtg gccggccttg acggcgctca
    35461 cccgtccggc gagcggacgg ccgagcaagg cgaggtcccc gatcaggtcc aggagcttgt
    35521 ggcggacgaa ttcgtcggga aaccgcagcg gtccgttcat caccgactcg tcgtccatga
    35581 ggatcgtatt ttcggccgag gcgcccaggg ccaggccccg ggaccgcagg ctctcggcgt
    35641 ccttgaggaa gccgaacgtc cgggccgggg cgatttcccg gacgaacacc ccccagcgga
    35701 tcggccgccg caacgtctgc ccgccgatgg ccgggtgagg gtaatcgatg gtgtaggaca
    35761 gctcgagccc gccgtcctcg atcggagggg caaagtcgat cgccgcgccc tcgtcggcga
    35821 gggcaaacgg ccggacgatc ctcaggaagg gctggaaatc gggcagcctc tcgatcccgg
    35881 ccgtctggag agcccgggcg aacggctcgg cgctgccgtc catgatcggg atctcatccg
    35941 cgtcgagctc gatcgtcagg ctgtcgatgc cgggaaccca cagggcggcc aggagatgct
    36001 cgacggtccg gacgcggaaa cggtcgccga ccaaagtggt gctgttctgg gaatcgattc
    36061 ggccggtggt caggggcagc tcggcgttat ccaaatcggt ccggcggaag atgattcccg
    36121 ggatcgacga cggcagaagg cggaggcgga cggatcggcc ggaatggaca ccgacgcctt
    36181 caaaaaagac aggggcagcc agtgttttgg aacgggtcat tatttctctc gtaaggacat
    36241 tctatcatgg atttcccgag agggagagac cagggcccgt tctcctcgct tcttttcgcc
    36301 ggtttcgtcg ttttccgccc gaaggacgac gggaaatcgg agtcttgccg ggacaagtat
    36361 tctgtctccc tgttttttgc taaaatccgg agtatgaaaa cccagatcga agatctcctg
    36421 acgaaggtcc gccggggaac cctgaccccg aggaaagccc tggccgcgct gaaggattat
    36481 ccgtttcagg acctgggctt cgccaaggtg gatcaccacc gggaaatccg taaaggatac
    36541 cccgaaatcg tctacggcct gggaaaaacg gacagccaga tcgtccaaat cgccgcagcc
    36601 atcgtccaaa aaggcagcaa ccttctgatc acccgggtgg aggcatccac ttacgccgcg
    36661 gtcaaggaag ccgtccccaa ggccgcttac aatgccctgg gaaaaatcat ttatctgagg
    36721 aagcgcccgg ctcctcccgg ccggggaatg atcgccgtct tgacggccgg gacgtcggat
    36781 attccggtcg ccgaggaggc cgcggtcacc tgccgcatgc tcggcaaccg ggtgcagcgg
    36841 ttttacgacg tcggcgtggc cggcctgcac cgccttctga gcgaatacgg ccggctaaag
    36901 aaagcccggg tcatcatcgt ctgcgcgggc atggagggcg ccctccccag cgtggtcgcc
    36961 ggcctgttct ccgtccccat cgtcgccgtt ccgacgagcg tcggatacgg ggccagcttc
    37021 aagggcgtgg ccgcccttct ggccatgctt aattcctgtc cggggggcgt cggcgtggtc
    37081 aacatagaca acggattcgg ggcggcctac ttggccagcc tgatcaatcg ctagaatgaa
    37141 ggagacgcaa gatccatttc actcaatttc tccttgccga tccagccgcg cgacacgcgg
    37201 cggcggcttt tgaaatcagg gaaatgggtc ttgtgtctcc tttttattat ctcgcttgac
    37261 aatccgtagg ccggacctta tatttacaaa aaattcgcga aattattctt tcattgaagg
    37321 cggccgtctt ttaaaatgga gattctcgac caggtccgga acgcggccaa tatcgttgaa
    37381 atcgccggcc agtacacggc cctgagacaa cgcggcaaaa aacacgtcgg tctctgcccc
    37441 tttcactccg aaaaaacgcc gtccttcacc gtcgacgccg acaagggtct ttaccactgt
    37501 ttcggctgcg gggccggcgg ggacatcttc accctggtga tggagaagga gaatctcgac
    37561 ttcccggaag ccatcgcctt cctggccgag aaataccaca tccccctccc cgaaaagaaa
    37621 cgtctctcgc cccaagccct caagctcgag gaacaactcc ggaaggtcct ggagggagcc
    37681 ctatccttct tccggaaaag cctccgggaa acagccgagg ggaaaaaagc cctggaatac
    37741 ctcaggggcc gggggctgtc cgccgctgtc atcgaggaat tcgccttggg atacgccccc
    37801 aattcctggg acgccctgat caaatacttc aaggccaaag gcgtcgcccc ggccctgctg
    37861 gagaaagccg gcctggtcgt tcccgggcgg aagcagggcg aattctacga ccggttccgg
    37921 ggacgggcga tcttcccgat tttcaccgtc accgggaaag ccgtcgcttt cggcgggcgg
    37981 tccctattcg gccaggatcc gaaatatctc aattcgcccg atacgccctt atattcaaaa
    38041 gggcacctgc tttacggact gaacgtcacg aaggacgcca tccgggaggc cggggagttt
    38101 atcctggtcg agggatacac cgattttctc agcctttacc aagccggacg ccggaacgtg
    38161 gtcgcttccc tggggacggc cctcacctcc cgccaggtcg ggttggcgat gcgtttcgcc
    38221 ccgaaagccg tcattaatta cgacggcgac ccggccggac ggacggccgc tttccgagcg
    38281 gtcccgctgt tcttcgaaaa gggcgtggaa acccgcgttc tcatcctccc ggagaatctc
    38341 gatcccgatg gcttcctccg gaagaacgga gcggccgcct acgaccttct ttaccccggg
    38401 gccgccccgg gcctgaaatt catcatcgat tacctctccc gggagaaacg cctggatgtc
    38461 cccgaagtca agacgcgggt cctccgggcg gtgatggaca tcgtcgaagc cgtcccggac
    38521 gcgatcgtcc ggtcggaata cttccgcctt acggctgaac atctcggagt ggatgagtcc
    38581 cttcttcgtt ccttgtccca ggtaaagccg ggcgaggcgg ccgcccggcc ggcggagaca
    38641 ttccttcccg ccgagaagcg gcttctccaa atcttgatgg aaaacgccga tctccggcct
    38701 gatatcttct ccgagctctg cgaggaagac atcgccggct tgaaaagcga gccgattttt
    38761 gccataatgt tgcgcttatt cgcaaatgaa caggattttt ccctgaacga gatgcataag
    38821 agcgtcgagc cggggctcgc ccgggagttg tcccaggctc tcctcgaaag gcgcgaagcg
    38881 ccgagcctcc aggaagcgct cgattgcctc tgggccctcc gagtcgccgg caaggag
//
LOCUS       DAOOXH010000013        37829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_917_length_37829_cov_8.885159, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000013 DAOOXH010000000
VERSION     DAOOXH010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 37829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37829
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_917_length_37829_cov_8.885159"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PKV64_03000"
     CDS             complement(<1..358)
                     /locus_tag="PKV64_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPN16084.1"
                     /translation="MTMEKSKKEGEAALDVLRHSAAHLLAHAVLNLFPGTQAGIGPAV
                     ENGFYYDFLRDEPFSSDDLDAIEAKMREIAASDTPIVREMLPKEEAVRLFEDRGQTLK
                     VELIKEKGGDVVSLYRQ"
     gene            complement(530..604)
                     /locus_tag="PKV64_03005"
     tRNA            complement(530..604)
                     /locus_tag="PKV64_03005"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(570..572),aa:Val,seq:gac)
     gene            743..1261
                     /locus_tag="PKV64_03010"
     CDS             743..1261
                     /locus_tag="PKV64_03010"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPN16085.1"
                     /translation="MPPSTPLGYNPGMIGILSDSHDNLDALEKAIRFFQDAGCALLIH
                     AGDFVAPFSARVLNRSGCGLKAVFGNCDGEKEGLKAIIGSFGTIRRAPYCFFYEGKKI
                     ALTHLDAPAGGLAARREYDLVVYGHTHRPEIRTAGSTLLVNPGETGGQVTGRSTVALY
                     DPATGRASIHTL"
     gene            1702..3006
                     /locus_tag="PKV64_03015"
     CDS             1702..3006
                     /locus_tag="PKV64_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HPN16086.1"
                     /translation="MKNVIIMGAAGRDFHNFNTYFRDNPNFKVVAFTATQIPDIAGRK
                     YPAVLAGKMYPDGIPIFPEEELTDLIRKNNVTKVYFSYSDTSHEYVMHRTSQVQAAGA
                     SFILLGPVDTMVKSVKPVISICAVRTGCGKSQTTRKVGAVLIAKGRKVAAIRHPMPYG
                     DLAVQKVQRFAAYEDLDKHHCTIEEREEYEPHIKAGIIVYAGVDYEAILREAEKEADV
                     ILWDGGNNDFPFYKSDLEIVVVDPHRAGHELLYYPGETNFRRAQVIVINKMDTAPKEG
                     VDKILENIKAYNPGAIVIKADSPTHVENGEQIRGKRVLVVEDGPTVTHGGMPFGAGVV
                     TAEKYGAGELVDPRPFAVESIAATYAKYPHMVKILPAMGYGEKQVADLAATIDKVDCD
                     LIISATPIDLNRLIKTRKPMLPVGYDLQEIGSPTIEDVLQKF"
     gene            3025..4002
                     /gene="arcC"
                     /locus_tag="PKV64_03020"
     CDS             3025..4002
                     /gene="arcC"
                     /locus_tag="PKV64_03020"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019849526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPN16087.1"
                     /translation="MSAVKRRLALIAFGGNALLSEKQKGTQEEQIANAEDAARLMAAV
                     VRKGYELIIVHGNGPQVGNLLIQQEAAADHIPPYSLDLCDAMTEGSMGYMLERALVNE
                     FRRHSIDKEATTVVTMVLVDKNDPAFAKPTKPVGPFYTAERAQGLKKEKGWTMVEDAG
                     RGWRKVVASPKPIDIVPKWIINDLVQAGRIVIAAGGGGIPVILNGNGLYEGIEAVIDK
                     DYASSLIAREIGVDLFIILTAIDRVCLDFGKPGQRDVAVLSVSEARRFLSEGQFPPGS
                     MGPKIRAAIEYIEAGGKEVLITDAAHLKAALLGRSGTRIVRDDHKTEGA"
     gene            4004..4921
                     /gene="argF"
                     /locus_tag="PKV64_03025"
     CDS             4004..4921
                     /gene="argF"
                     /locus_tag="PKV64_03025"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPN16088.1"
                     /translation="MKKDFISIHDLTRYEFFELMDQTKKIKDDPDEYRKKLKNQVLAM
                     IFEKPSLRTRMTFEVGMLELGGKAIYLSPSEIQIGKRETVEDVAHNLERWVDGIMIRT
                     FGHDNVIRLAQAASVPVINALTDLLHPCQAMADFFTLKEHKGNISKIKLAWVGDGNNV
                     CHSLMFAAAKAGCKMSAAVPKGYEPNPEIVRLALEDGKGSDFELIVTNDPLEAVAGAD
                     AIYADVWTSMGQEAEKEERLKLFASFQVNEALMSKAKDDCVFMHCLPAHRGEEVTAEV
                     FDGPRSIVFDEAENRLHAQKAIMLALMGK"
     gene            4934..7078
                     /locus_tag="PKV64_03030"
     CDS             4934..7078
                     /locus_tag="PKV64_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16089.1"
                     /translation="MEKDNALVQVTPDDISFIEKELAGSRKPTTTKDLAGKLAYQKTA
                     GERTRAVKLYDPACSYEVGDTICKEYDEDLIVGAKVHEHFQGAVVLTVIRKFFYKDFD
                     CEMLEVDYTGGGTFRKHIDYMKKSKIQVMLPANEGGTNRPPRIMPQAEDPRLTELPMT
                     ERDLKTLERLLRAALAKSPAFFAWGDRWQLTAAQPSLPEETIKEIEAHLAETKASAAT
                     AELVSTFFRMEPSHDLFDLHCLALNRLLETKYRRDFVLVQPVDWGRWMLKSVLSAMPE
                     GLPLAAPMAALPPFENEETIAVTPFHEFPLKVYLGWREILSGGVKIPKGFNKELSQAR
                     EYVFTDADEKKSYTVHYFPQQGYFLGLREYFETTAIPQGTSMTLEKTGPIQFNFWLKK
                     SKKKIQVARMAYETKGDTFAEAGEMATLAMPNKIIYLEREHLERLTALIPQRTGLDLK
                     GLLILVFKQFSLQSANYSLHYLRAYHLVDILRRTTQEDVEFTLLNSPEFEKSDKKKGI
                     FYYHETPSETPVEEAEGEEAAAEEIISEEAGELTSDDFLEHEFTILEQAPPPAPPAAA
                     RKEEEAAAMAEAEAELEAFEAEKKVKIEKEAAPPPKKEKEKKKIGLESDRRPRSRKSE
                     RRVKEEELVELESVREALFAEKGAESEGLELPADKTEFEQPEVPVETAPPAGPGPLGA
                     FGGGLFAEKLKVALKKKRGEKAEEPDKPDETS"
     gene            7125..8477
                     /locus_tag="PKV64_03035"
     CDS             7125..8477
                     /locus_tag="PKV64_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16090.1"
                     /translation="MIGRFTVAAPLAFFLVSVPSSLLGREETSRPAGRFRAAFGLDSL
                     ERKYYQPEFRLSAPLGGPGGNGLFLDLSYLQKINGDMEGPIDFWVRAGWAAPLSAGVE
                     AEASLNHFCRHQTSLFSDYILNLNEIVGRLRLRHGGFRAGFGLGTYLGGTPGYDGLAV
                     FEAAGTGLLFPEISWAAEVKWVNFSEILYEAELAVALSPGAELVLAGLKTYRLPAATH
                     VGVRFGADGGGSRFLDAFDVTAGVYPFYDAYKVMIDGTFRLPLLRRESRRFFIDTAFS
                     SPLLTGSGFWGQFWPDRMMYSVTAEYERPVGRAFASVYGRYIADMPVDKAVRFRAGAS
                     TGLAIRNQPDFNRLDGPIRFEARAGFDFKFAYDFGMKIGLNTTGPADLKAGLDVLFEA
                     NDARRTVEARVFLDFGRDVSVRPFVGVRRVVPLAGDSASDGTFPRIVAAGISFYTWFD
                     "
     gene            complement(8474..9304)
                     /locus_tag="PKV64_03040"
     CDS             complement(8474..9304)
                     /locus_tag="PKV64_03040"
                     /inference="COORDINATES: protein motif:HMM:NF020672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HPN16091.1"
                     /translation="MRASHPPIPKRLPAGDGPKIIALAKAYGLQGLRQKDLAGVFPPD
                     FQGLTDLAKALESEGRIRILSFAPLHLVAREAIDYFEEKFVAYLEKFHSSHIKERGVA
                     LSKIQERFEIPRQILLLCLKTLVHEGKLREDHGFYALADFVRELPEREERILQKIEEE
                     CFAGRPRSVSPAEVCERMNLSPETRNQLLDILVERKRIIAGKDGYYVHRPWLEDLIEK
                     LRAKGPRDLPVAEFKEMTGLSRKYAIPLLELLDEMGVTRRKGAGREILEEPSSPSSRS
                     "
     gene            complement(9450..11570)
                     /gene="rnr"
                     /locus_tag="PKV64_03045"
     CDS             complement(9450..11570)
                     /gene="rnr"
                     /locus_tag="PKV64_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HPN16092.1"
                     /translation="MAKAQSDEARILNLLRRNPQGLKLRDIFKELRVDRKSRAKVEDR
                     LQSLVHRKLARRARDRWLLPPASDLRKGTFETFGRGFGFVVSEKGEDVFVPSRFSSGA
                     VHGDQVEVIVNASGRSGRLEGRVVRIVKKVKKRLLGIFGERFGRPFFVPFETPSAEEI
                     PIVSRGSFFPEAGEIVSVERADMKIVEVLGRPDDPGVDAQVIIRKYGLADAFSPAAEA
                     EAEQTAAVSAAETIAGRADFRSWKTFTIDGEAAQDFDDAVSISRRPSGGFRLGVHIAD
                     VSHYVRPGTALEAEAFARATSVYFPGLTLPMLPARLSNDLCSLRPRLERLAVSVVMDF
                     NAEGERIGTSFHPSVIRTVERMTYDSVFKIMENDPAEREARASIVPDILDMRTLARAI
                     RKRRLAGGSLDFDLVEPELVYQEGKVASVAALARNEAHELIEEFMVAANVAVAEYLRL
                     RGIPSLHRVHEAPAEEDLEKLRATLAFFHILLPESKRITSRDLQSAVRAAEGTRLEKF
                     VSVQILRAMRLAEYSSERSGHFGLGLKDYTHFTSPIRRYPDLVVHRLLKDDLAGRVPR
                     PAGLEAAAVHSSEQERKADAAEKDLVEWRIFRFLKERLGDVVSGLIVDFSRGGLIVEL
                     DEYFVSGLLAYDDLGGDYFAKRSRGALTGKRTGRQYELGQGLSVQIAAVDPVNRRMSL
                     VLPAEQEPAGLERKTSRGRKKRGH"
     gene            complement(11631..12287)
                     /locus_tag="PKV64_03050"
     CDS             complement(11631..12287)
                     /locus_tag="PKV64_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16093.1"
                     /translation="MSDEPSAAGFRERYFRGIADRFIALRGAPFSLSPADICLIEAWE
                     SAGIPLETVLEGLEAAFALRPGRGRAPGKIRTLSFCRAAVERALERSRERDVGRRSPE
                     LETRSTEKIEKARAAVDEFLGRRPAGFAALLPEFEKARSLLKAKRPDDEALERLDGKI
                     DAWIRDSAGPARLGDAEAAVDRDHPGLRGPEKESAARIRLVRNLREKHRIPYVSLYYY
                     "
     gene            complement(12304..13086)
                     /locus_tag="PKV64_03055"
     CDS             complement(12304..13086)
                     /locus_tag="PKV64_03055"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPN16094.1"
                     /translation="MLLACDIGNTSVAFGVFESRKLVREWRLRSDRDQTSDEYGVILL
                     NLLRMAGIDPAAVKAAIISCVVPPLTPVFQKLCGSLFGVRPFVVGPGLRTGMPILYDA
                     PAEVGADRISAAVAAYEKYGGPCIVVDFGTATTFDAVSAKGEYLGGAIAPGILISAEA
                     LYLKTARLPRIEIRKPDRIIGKTTVGSMQSGLYYGYIGLTQTIIDGLRKELGEKTKVV
                     ATGGFGEEIAAEVPAIDAGEPNLVLEGLRIIFERQKDRPSEP"
     gene            complement(13150..13923)
                     /locus_tag="PKV64_03060"
     CDS             complement(13150..13923)
                     /locus_tag="PKV64_03060"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPN16095.1"
                     /translation="MNDSVCFLDTCVSTNDEARKRAREGAPAGTVVVAREQTGGRGTK
                     GRSWFSPRGAGLYVSVVLRPGTDAALSLLPLAAGLAARRSVERTAGIPAKVRWPNDIV
                     WQGRKLGGILCESGLSGFRSRYAIVGLGLNLSQDESDFPEEWRARAGSLRLAAGRIID
                     REDVLAAFLEELEGAVAPLFEKRPEDVVDAFLLHAEAGPGEAIRLRTASGDVAGLFEG
                     LDIDGAIRVRTAGGTRRFVSAEIVSFGLDRAPSGESFGV"
     gene            complement(13934..14569)
                     /locus_tag="PKV64_03065"
     CDS             complement(13934..14569)
                     /locus_tag="PKV64_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN16096.1"
                     /translation="MSMKILLADASPVVRKAVETALPGAEFEVRTAPDGLAAIRILPE
                     FFPDAVLVALSLPGMDGYDVAAFLCSQPVYRTTALFLLPGAFEAVDVAKLSVIRHDGL
                     IRKPFDGETLLSRIRTAIDRKKELPSLPEDPFLEPQSLPETASVASGPVTPDSGNLPE
                     WTDDVERKIRDVIRQEILRNQAEMEERARDIVSAEFKKVLVEELKAVDGKR"
     gene            complement(14571..18050)
                     /gene="dnaE"
                     /locus_tag="PKV64_03070"
     CDS             complement(14571..18050)
                     /gene="dnaE"
                     /locus_tag="PKV64_03070"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPN16097.1"
                     /translation="MAPGFIHLHNHSEYSILDGAVRLDALVQAAVDHKMPAVALTDHG
                     NLFGAVKFQRIAKDKGVKPIFGCEAYIAPRSRRDRELRDGEPAHYHLLLLVENETGYK
                     NLCVLLSKAYLEGFYYRPRIDKELLAAHSKGLIGFSACLRGEIAYLFGQGREEAALRA
                     AESYAEIFGPGNFYIELMDHGLEPQREVNPKLVGLARRLGLPLVATNDIHFLGKADAA
                     SHDVLLCIQTNKKISDTDRIKFGSAEFYFKPPAEMERLFGEIPEALDNTFHVAARCDF
                     EFPKGTYFLPRFEPPEGKSLRDYFEEVVWAGFSERADKLRDLRAKGKLVHDLDAYKDR
                     LEREIQLVKQMQFEGYFLIVWDLLRTARSRNIPVGPGRGSAAGSLLAYCLQITDIDPL
                     EYDLLFERFLNPERISLPDIDMDFCARRRAEMISYVTEKYGQENVSQIITFGTMAARA
                     AIRDVGRVLEVPLSEVDKVAKMVPLGPDVKIASAVQDAGPLQEYVERNPRIKALLDIA
                     EKVEGQVRHPSIHAAGLVITPRPLTEFLPLYQSVKGEVTTQFAMSEIEAIGLLKMDLL
                     GLRNLTVIRDAQNLIAKDFGTAPDVDALPLDDGPTFDLFKAGNTDGVFQFESQGMKAL
                     LRSYKPEEFRDLIALNALYRPGPLKSGMANDFIERKFHPEKIVYDIPELKPILEETRG
                     LIIYQEQVMRIAVELAGFSLSKADLLRKAMGKKKAEIMTAQKQNFLDGAKRKGAISAS
                     KAAKIFDQIAKFAEYGFNKSHSAAYAALAYQTAYLKAHHPTHFMAALMTSEAERGATS
                     QIVKYVAECQVLGLEVRPPDINKSDFDFIVEDRAVRFGLAAVKNVGETAARALIAVRR
                     ERGPFQTIFDVVGHADGRIINRKVLESLIKAGAFDSFGLPRARSFRLIDNLIDYNREM
                     QKIRESTQSLLFGRDALAPPEIPAEIAAISEWDEMLKWSYEKEALGFYISGHPLSHYR
                     RILPRLTSHSTASFEEEADIPEEIRVAGVIADFQMKKTKKDERMAVFQLEDLTGRVEV
                     VVYAEPYKRYYHVLQEGSLVWIKGHYQLDGETHKIYLSHILPLEEAAQKLAKKFVVRL
                     PARPLNEAVLQDMREVLEKSPGECPAVFELETPSGHRVVLQSPELKGVTPTDDLLTSL
                     ETLLGPDSIRVDY"
     gene            complement(18055..19587)
                     /gene="guaA"
                     /locus_tag="PKV64_03075"
     CDS             complement(18055..19587)
                     /gene="guaA"
                     /locus_tag="PKV64_03075"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPN16098.1"
                     /translation="MNKILVIDFGSQYTQLIARKVRELGVYSEIWPPTAPVEAIRAAR
                     PQGLILSGGPQSVYGKGAPTISPEIFTLGVPILGICYGLQLMAHLLGGRVVPSPRREY
                     GFAALRLVRRSGLLSGLKDGEQVWMSHGDKVEKLPPGFRVSGRTGNTRAGVIEDPRRD
                     FYGVQFHPEVIHTKEGRKLLANFLFKIAGLIPDWNMGSFIERKTEEIRKTVGRGRVLC
                     GLSGGVDSLVTALLVHRAVGKRLTCVFVDNGLLREGQYAGLMEMFRVKLDLDVVGVDA
                     AAAFVKALGGVSDPERKRKIIGGLFIKVFREEARRLGGAKFLAQGTIYPDVIESRPVQ
                     GPSAVIKSHHNVGGLPKSHGFVLVEPLRELFKDEVRAVAVELGADRAFVAQHPFPGPG
                     LAVRIVGPVNEESLRIVREADDIVVREVRAAGIYDRLWQAFAVLLPVRSVGVMGDRRT
                     YQRVVVIRLVQSVDGMTADWFPASPGLLARISNRIVNEVAGVNRVVFDVTSKPPGTIE
                     WE"
     gene            19730..20932
                     /locus_tag="PKV64_03080"
     CDS             19730..20932
                     /locus_tag="PKV64_03080"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPN16099.1"
                     /translation="MSFEELSRELAVQTDTKIVLFVIDGLGGLPRNGRTELETAAAPN
                     LDRLAARSSCGLTDPVLMGVTPGSGPAHLALFGYDPLRHRLGRGILEALGSDVEVREG
                     DLVARGNFATLKDGLIADRRAGRIPTSECERICAKINAARAGMSNPEITLFAGKEHRF
                     VARFRAPGLSDALTDADPQKDGKPRVPASALDAASEKAASIVNTFLDEILGILKDEPA
                     ANAALLRGFSVQPTIPTMADLFKLRPAAIANYPMYKGLARLIGMHVYKVGPEPEDLFS
                     ALEARWKDHDFFYVHFKKTDAAGEDGNFEAKVRAIEEADRFLPRIEALKPDVLVVTAD
                     HSTPALMRGHSFHPNPFLLAAPTALPDDVVAFSERACAKGILGRFPSLRAMPLILGHA
                     GKLKKYGA"
     gene            21027..22229
                     /locus_tag="PKV64_03085"
     CDS             21027..22229
                     /locus_tag="PKV64_03085"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPN16100.1"
                     /translation="MSGRRGRLWTIFPVLILSLAAAPAEKTDVPVLSEVYNVRSYAHP
                     NYTRIVLDVGAPREYVWNETLTPPEITVDVIRARLNPIVPETVTPAGAGYIRRLRLAA
                     KNPTTVRLTIEADWDRVRRYDVFNLFDPFRIVIDIYPKDPAAGPAAAAPPAEKIPKPA
                     QPAGGGYSLARQLGLGVRTIIIDPGHGGSDPGCLDPAGPKEKDLTLDIALKLKAILEA
                     ESDLSAVLTRETDIFVPLEARTIVANQRKADLFISIHVNAYRLKARQGLETFFLNFSP
                     DPAVNELAAKENATTTKTIGEMDKIVKKVVQNSRIIESRELARTVQSALASHLSRHYA
                     GVKDLGAKGGPFWVLLGSEMPAILIEAGHLSNPEEIKRLQSAVYRQNIARGIFEGIKA
                     YIRSLGKG"
     gene            22234..23130
                     /locus_tag="PKV64_03090"
     CDS             22234..23130
                     /locus_tag="PKV64_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPN16101.1"
                     /translation="MKRRTFLKSAVWGGAALSAVPGLIGRPAAAPGPPDLAVIQGNSP
                     ADIARAAVEALGGMKRFVSRGDVVLIKPNIGWDRTPEMAACTNPDIVRTLVELSFAAG
                     AKKVAVMDNTTNQAQRCYVRSGIQAAAREAGAEVPFINPRRIKKMTIGGAWLKEWDVI
                     QDFVECDALINVPIAKHHNLCRVTLGMKNWLGAIGDPRNRLHQRIDEASADLAAFFKP
                     KLTVLDAWRILIRNGPQGGRPSDTKLLKTAVAGTDAAAVEAMGAVLFDIVPAELPLLR
                     FAVERNIGRIDLEKMNIEKRTL"
     gene            23111..24688
                     /locus_tag="PKV64_03095"
     CDS             23111..24688
                     /locus_tag="PKV64_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPN16102.1"
                     /translation="MKKERSKKFRRLQIARIAVQTAFLGLFFLLLFGAHFTGEDYIGR
                     VEAFFHFDPLLAAATILASRVFLVYFAWAALTVLVTVLFGRIVCGWICPLGTVHQLFS
                     WIFKKARFLRPKKPASGGLWIKYAVLAFVLVAALFGLNAAGYLDPLSFLTRASAAGLI
                     PAFGLGADALLGAAYAVRLEAFGDTLAQALSTLTLNTTFRQGFLLGLIFLGAVLLNKI
                     RERFWCRYVCPLGALLGLFSRYNLFKLKIDESKCTQCGLCSIHCQTQAAPFPAGGWKS
                     SECVYCETCAAICPERAITFPVRLGAEKPPAVALTRRRLLATSFLAFFSVPFFRITSA
                     RTRADEHLIRPPGSLPEPAFLRTCIKCGECLKACPTNGLQPALTEAGPEGLWTPVLVP
                     KIGYCEYYCSLCTQVCPTGAIKELTVPEKTTVKIGTAWIDKNRCLPYFLGRPCIVCEE
                     HCPVSPKAIKFILVETKRPDGTTAVEKAPIIDPELCIGCGVCENKCPVADRPAIFVTS
                     VGERRSKENQLLLNVGE"
     gene            24824..27427
                     /locus_tag="PKV64_03100"
     CDS             24824..27427
                     /locus_tag="PKV64_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPN16103.1"
                     /translation="MKQRTAAFILTLALFVPSIAAPGPRPAPAAETAAVTSPETFFGF
                     RLGADRRMAPWDKIVAYYRLLARESDKIKVVDLGPSTMNQPFLLVVISSSRNLGRLEE
                     LRKINLRLSDPRDLDETAAEKLIAEGKAVICQSMSLHATEIGGTQMAPELAYDLVSRA
                     DEETARILDNVVFLLVPSFNPDGAVMVYDWYQKTLGTEYEGSDLPWLYHKYAGHDNNR
                     DAFQTNLVESRYMARLLFRDWIPQAYVDHHHMGSYGPRIYVPPYAEPIRPLADPLIWR
                     EHSWYGAHIAYKEEEAGLSGVINAAQFSGWGHFGFHWITPFHNIAGMLTESADARLAS
                     PLYIHPHQLKGDDRGFPAYEAQTTFPNPWPGGWWRLRDIIDRQKISAWAVLDLAARNK
                     ETVLRNAFLKARRQTERGAQGKPAAYIIPAVQHDPLTAVKLVNKLLDQGIEIRRAAEA
                     FRAADGKPYAAGSFLVPLAQPKMGLIRYLLGRTLYPDNSFTRRPDGTPESPYDMATDT
                     MAEFMGVRVEALDEAVTGNFETLTGALAPAGRAAESAAGHSLDGRLNDAFAAAGRLLE
                     RGIRLRRAEESAGGLRPGDFLVPAGTSSALLSEIARTTGVDFRALEPGLDRTGREVKR
                     QRLGVYQRYWGGNMDEGWTRLLLEEFAIPYTTLRDAEINKGGLNAAFDVILLPDDAPA
                     LITGEGLDHRPGLLESTPPEYRSGIRAEGFEALKSFVAKGGTLVAFGRACGLAVEKFG
                     LPVRNVLAGINRKDFWCPGSTLRVTYDPKNPLAYGQPDEGLVVFQMGNPAFEILPTNH
                     NERAEIAAAFASRDLLQSGWLLGESRLAGRAAVVSVRHGEGRVVLFGIRPQHRAQTHG
                     TFKLFFNALIR"
     gene            complement(27461..27841)
                     /locus_tag="PKV64_03105"
                     /pseudo
     CDS             complement(27461..27841)
                     /locus_tag="PKV64_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522549.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(28129..29103)
                     /locus_tag="PKV64_03110"
     CDS             complement(28129..29103)
                     /locus_tag="PKV64_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPN16104.1"
                     /translation="MILCFIEIRDGKIKKSSLETLVAGRRAAEALGFPAEAVLVGSAP
                     EGAAAEIGAAGAAKIHHLQNPALTDYSSQGFALAVEGLVRETAARAVFFPATAMGRDL
                     APRLAARLGVPLASDCTAVSVSGGRLVFTRPIFAGKALLTLALPAGPAIATLRPNVFP
                     LDAEPGPEAEVVPHAVAIPEGAVAGKVAEILSESGAAIDVAEADIVVAGGRAMKGPEG
                     FARLSDLAAVLPRAAVGASRTAVDAGWIGHSHQVGQTGKTVSPNLYFAFGISGAIQHL
                     AGMSSSKVVVAVNKDPEAPIFKVADYGIVADLFQVVPALIAEIKALGS"
     gene            complement(29115..29897)
                     /locus_tag="PKV64_03115"
     CDS             complement(29115..29897)
                     /locus_tag="PKV64_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPN16105.1"
                     /translation="MNILVFIKRVPDTESRIRIDREAKSIVEEGLNFVLSPYDEYALE
                     EAIRLREAKGGKVTAVSFGPDEALSTLRKALAMGADEAVLIKDDLREAYDPLRIARVL
                     AAFVRARQPDAGLILFGRMSVGSDNAAVPPMVAEMLGLPQATVVTKLEISGQEAAACR
                     EIEGALEKVAFRLPAVVSAQKGLNEPRYETLKGIMAAKKKTVPVVTLAELGLSGEEAF
                     PRVEILSLELPAAKQAGKILPGTAEEAARELVRLLRNEAKAL"
     gene            complement(29977..31254)
                     /gene="fabF"
                     /locus_tag="PKV64_03120"
     CDS             complement(29977..31254)
                     /gene="fabF"
                     /locus_tag="PKV64_03120"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPN16106.1"
                     /translation="MPNSRETRDHRHDLRRVVITGVGLVTPLGTGVSDVWENVISGSS
                     GIGPITRFDASRHATRIAAEVKGFDPLRFVDRKEIRKMDPFIQYAIGAAVLAAEDAGI
                     PPDLLAGERCGVYVGSGIGGIGTIEEYHKILLEKGPDRISPFFLIATIINEASGRISI
                     RFKSRGPNSASVTACASGAHAVGDAFRIIQHGLADRMIAGGAEAPITPLSVAGFCAMK
                     ALSERNDDPGRASRPFDKTRDGFVIGEGAGICLLEELGAAVRRGARIYAEVVGYGMTG
                     DAYHPAAPAEDGDGAVRAMQAALDDAGVPPADVQYINAHGTSTPLNDRIETAAIKKVF
                     GSQAVRLAVSSTKSMTGHALGAAGGIETAITALAIRNQTMPPTANYEVPDPDCDLDYV
                     PGRARSAEIVYALSNSFGFGGTNATLLLRRCDG"
     gene            complement(31244..31486)
                     /gene="acpP"
                     /locus_tag="PKV64_03125"
     CDS             complement(31244..31486)
                     /gene="acpP"
                     /locus_tag="PKV64_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPN16107.1"
                     /translation="MDRDELLKKMKTIVADKLSISEDQVTENASFIEDLGADSLDTVE
                     LVMALEDEFGLDIPDEEAEKLTTVGKAMEYILGHAK"
     gene            complement(31516..32256)
                     /gene="fabG"
                     /locus_tag="PKV64_03130"
     CDS             complement(31516..32256)
                     /gene="fabG"
                     /locus_tag="PKV64_03130"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPN16108.1"
                     /translation="MIFKDKVTVVTGASQGIGETIARDFAAEGAVVVLVDIQKEKLEA
                     VAASIKAAGGRASVREANVAAFAQAEAAAASVVQEFGKIDHLINNAGITRDNLLLRMR
                     EEEWDAVVAVNLKGIFNFTKAVLRSMLSARSGRIVSLSSVAGAMGNPGQTNYAATKAG
                     VVGFTKSLAREVGSRGVTVNAVAPGYISTPMTLGLPEAVKQSFLALIPLQRFGEPADV
                     AKAVRFLCSDDAAYITGQVIHVNGGLYM"
     gene            complement(32256..33266)
                     /gene="plsX"
                     /locus_tag="PKV64_03135"
     CDS             complement(32256..33266)
                     /gene="plsX"
                     /locus_tag="PKV64_03135"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPN16109.1"
                     /translation="MKIAVDAMGGDLGLRITIPGAVQAAKENDLDVVFVGAKEQISPE
                     VARWGGKPSDFPIIDAPESVGMGDGMFALRRKKRSSIRIGIQLVRDGQADAFVSMGNT
                     AAVVAMARQILGPLPGVNKPALSLLIPTLKGHTLLLDVGANANCQTEDLAQFAVMGKV
                     FMEQVMGRPNPSVALMSIGEEEGKGNELTRQTFDRLSVSGLNFIGNIEGKDLYAGKAD
                     VIVSDGFTGNVALKVSEGVIETLFHMARREITKNLFAKIGLLLMKGHLKRIYKRVDYS
                     EYGGAQLLGLNGMCIVGHGRSNSNAVKNAVRLAREFVVKRVQDRIREELARTVAPGEV
                     KA"
     gene            complement(33284..33466)
                     /gene="rpmF"
                     /locus_tag="PKV64_03140"
     CDS             complement(33284..33466)
                     /gene="rpmF"
                     /locus_tag="PKV64_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPN16110.1"
                     /translation="MPNPKRRHSHARKGRRRAHDFMTVPSFSSCPNCGTPKMPHHACP
                     ECGYYRGRPVIKAAEK"
     gene            complement(33467..33988)
                     /locus_tag="PKV64_03145"
     CDS             complement(33467..33988)
                     /locus_tag="PKV64_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPN16111.1"
                     /translation="MIIDIDKLPKDGLDISRNFDFPSVDLVEEDAVFLQPVQAELSVK
                     RMEEEVFIKGRVKTVLRFTCGRCLAPFEFPVDSRFDLVFLPEEMQELKEEFDDEDADR
                     LFYRSRRIDLREVILEQLNLTFPARPLCSPGCEGLCAVCGKIRREGACGCQVKETDVR
                     LEPLKILRKDKGK"
     gene            34179..34442
                     /locus_tag="PKV64_03150"
     CDS             34179..34442
                     /locus_tag="PKV64_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16112.1"
                     /translation="MNGLSTTILYSGISFLVQTQEGGPQTHSLETLIYRRGQLVYSRK
                     SSCTALLDDPERAERLTHLIRDQHQAVLDDIAGGRLDIPLLKS"
     gene            34445..37132
                     /gene="polA"
                     /locus_tag="PKV64_03155"
     CDS             34445..37132
                     /gene="polA"
                     /locus_tag="PKV64_03155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPN16113.1"
                     /translation="MPGKILYLIDGNSLLYRSYYAVQRLSTAAGFPTNAIYGFLITLR
                     KILDEAAPGHLGVVFDAPGEKIRREVYQDYKAHRKPMPDDLAAQLPKLKEVLQACRIP
                     LLESPRHEADDVLGCLALQAAAAGFETVIVTTDKDLFQLVDSKIKVFNPAKEKFFDEK
                     TVGKEFGVPPNRVVDVLSLWGDPSDNIPGVPGIGEKTAKALITEHGSLDAILEHPERI
                     KNLRLREAVKNNRDKIEISRKLATIECALDVPFDPVAFQVEEPDKPALARLYSELEFT
                     SLAAGISAGAPLEKKKTRVYRTILAVLDLRRLTGRIREAGFVALDTETDHISPTRARL
                     VGMSFSLEPSEAFYLPLRHDYLGAPAQIPLGQALDILRPVLEDPKIRKIGHNIKYDLI
                     VLRREGLDLQGLDDDTMILSYLLEPNWGRHNLDRLAAAYLQETAIPYQAVVGKGKNEI
                     TMNRAPVESAAPYACQDADFALSIARILRPRLEQEGLDSVYRDLERPLIRILADMETA
                     GVRVDEAALRSVSAELEASLRLLEKQIYEHAGLEFNINSPAQLSSVLFQKLLLPAPKK
                     ARGSGAVSTAIDVLDGLRDLHPVVPLVIEYRQTAKLKSTYADALPALIDPADGRIHTS
                     YNQAVTSTGRLSSSDPNLQNIPARGPIGKRFRAAFIPEKGRLLLAADYSQIELRVLAH
                     LSEDPALIDIFRRDRDIHAETADRVFGRNAGGTDEERRRRAKIINFSLIYGASAFSLA
                     RELETSTREAQEYIDKYYERFPGVKAYLEGLVASAASRGYAETLFGRRRPVPELKQSD
                     RTARDAGRRIALNMPIQGTAADIMKKAMIAAGAELGRRALAARMILQVHDELVFEAPE
                     KERSILDGLVRNVMESACPGFRVPLKVSLGWGASWAEAK"
     gene            37179..37409
                     /locus_tag="PKV64_03160"
     CDS             37179..37409
                     /locus_tag="PKV64_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16114.1"
                     /translation="MSREQTEAPGKAGPAGRFLFISEILNRPVVSAAGETVGRLHDPK
                     VRLNEPYPPIVGLAVRKRRLSPHPRFHAHPDQ"
     gene            37532..>37829
                     /locus_tag="PKV64_03165"
     CDS             37532..>37829
                     /locus_tag="PKV64_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN16115.1"
                     /translation="MTEAAGRPLILIVEDEIIIAMEIQVRLESAGFAVCGLASSGEKA
                     IALAREKNPDLVLMDITLKGPLDGLETAGRIQAEKDIPVIFLSATDDEAVLRRIR"
BASE COUNT         7120 a        12341 c        11558 g         6810 t
ORIGIN      
        1 cctggcggta cagggagacg acgtctccgc ctttttcctt gatcagctcc accttgagcg
       61 tctgccctcg gtcttcgaac aggcggaccg cttcctcctt cggcagcatc tcccggacga
      121 tcggtgtgtc ggacgcggcg atctcccgca ttttggcctc gatcgcgtcc aggtcgtcgg
      181 aggaaaacgg ctcgtcccgg agaaaatcat aataaaaacc gttctccacg gccggcccga
      241 tgccggcctg cgtccccgga aacaggttca ggacggcatg ggcgagcagg tgggcggcgc
      301 tgtggcgcag aacgtccagc gccgcttctc cttctttttt cgatttttcc atagtcattt
      361 caaacgtccc gttcctgaag acaaaagtcc cgcttcggac gtttcgccgg ataacgaacc
      421 cctcttctat ggagaacggc cggcccggcc atattacgcg acggccggcg tcgtaaacgt
      481 cgaaaacgtc ttctgtttca tcggattccg tacatcgctc cggcaaaggt ggtaggcgcg
      541 gaggggattg aacccacggc ctcttccgtg tcagggaagc gctctcccac tgagctacgc
      601 gcctatatcc gtcaccaaag cccaattata ttaatgaaaa ccaaagtcaa aatcaaccgg
      661 aaataagaga atagagcccg gggggcgaaa gagaccgggg aagcggccgg caggagaatc
      721 ccggccgccg cggcgattca cattgcctcc ttcgactccg ctcggctata atccgggcat
      781 gatcgggatt ctatcggatt cccatgacaa cttggacgcc ttggagaaag cgatccgctt
      841 ctttcaagac gccggctgcg ccctgctcat ccacgccggc gacttcgtcg cccctttttc
      901 cgcccgggtc ctgaaccggt ccgggtgcgg cctcaaggcg gttttcggca actgcgacgg
      961 tgaaaaagaa gggttgaagg cgataatcgg atcctttggg acgatccgaa gagcccccta
     1021 ttgttttttt tatgaaggga aaaagatcgc cttgactcat ctggacgccc cggccggggg
     1081 actggccgcc cgccgggaat acgacctagt cgtctatggt catacccacc ggccggaaat
     1141 ccggacggcc ggatcgacgc tgctcgtcaa ccccggagag accggaggtc aagtgaccgg
     1201 tcgaagcacg gtcgccttat acgatccggc gacgggccgg gcttcgatac acaccctgta
     1261 ggaggcggac atgtctcact ccgtttccaa ccggcaaaag cccgtctggc ggcagattcg
     1321 caagcccatc ccgcccccgg gacgccccca gacgacgaga aaaggcaaaa aagcatacaa
     1381 ccgcaaggat aaatcctgga agacggatat tctttaaaat aaaccaagac cagggctgtt
     1441 ttcatgaaaa agggggcgct ccgccgtaaa aggaatcgaa tcgggcggcc gccggcttca
     1501 cggcccgggc aaccaaggca tcatgcccgg cgcggctcca gccaacttca aggaaaatcg
     1561 gccggttcca agcccggccg agcagatttt agtgaaagcc cccttccggc ggcaagttgc
     1621 gtgaccggag cggctgttgt atactatctc cttatttttt taaaacttaa cgttcctatt
     1681 ttttgtaaag gagtagtttc tatgaaaaac gtcattatca tgggcgcggc cggccgcgat
     1741 tttcacaact tcaacacgta tttccgagac aacccgaact tcaaggtcgt cgctttcacc
     1801 gccactcaaa tcccggacat cgccgggcgg aagtacccgg ctgttttggc cgggaaaatg
     1861 tatcccgacg gcattccgat tttccccgag gaagagctca cggatctgat ccgcaaaaat
     1921 aacgtcacca aggtttattt ctcttatagc gacacgtccc acgaatacgt catgcaccgg
     1981 acttcccagg tccaggccgc cggggcgtct ttcatcctcc tcggccccgt cgatacgatg
     2041 gtcaagagcg tcaagcccgt catctcgatc tgcgccgttc ggacgggctg cggcaaaagc
     2101 cagacgaccc gcaaggtcgg cgccgtcctg atcgccaagg gccgcaaggt ggccgctatt
     2161 cggcacccga tgccctacgg cgacctggcc gtgcagaagg tccagcggtt cgccgcctac
     2221 gaggacctgg acaagcacca ctgcacgatc gaggagcgcg aggagtacga gccccacatc
     2281 aaggccggga tcatcgttta cgccggcgtt gattacgagg ccatcctccg ggaagcggaa
     2341 aaagaagccg acgtcattct ctgggacggc ggcaacaacg acttcccctt ctataaatcg
     2401 gatctcgaga tcgtcgtcgt cgaccctcac cgggccggcc atgagctcct ctattatccg
     2461 ggcgaaacca acttccgccg ggcccaggtc atcgtcatca acaagatgga cacggccccg
     2521 aaagagggcg tggacaagat cctagagaac atcaaggcgt ataatcccgg cgcgatcgtg
     2581 atcaaggccg actcccccac tcatgtggaa aacggcgaac agatccgggg caagcgcgtg
     2641 ctcgtcgtcg aggacggccc gaccgtcacc catggcggca tgccgttcgg cgccggcgtc
     2701 gtcacggccg agaaatacgg cgcgggcgag ctggtcgatc cccggccgtt cgcggtcgaa
     2761 tcgatcgccg cgacctacgc caagtatccc cacatggtca agatcctgcc ggcgatgggc
     2821 tacggcgaaa agcaggtcgc cgatttggcc gcgacgatcg acaaggtcga ttgcgacctg
     2881 atcatcagcg ccacgccgat cgacctcaac cggctgatca agacccgcaa gccgatgctt
     2941 ccggtcggtt acgatctgca ggaaatcggc tctccgacga tcgaagacgt ccttcagaaa
     3001 ttctaaaccg gaggccccag tcttatgtcc gccgttaaac gccgcctcgc cctgatcgcc
     3061 ttcggaggga acgcccttct ttccgaaaag cagaagggca cgcaggagga acagattgcg
     3121 aacgccgagg acgccgcccg gctgatggcg gccgtcgtcc gcaagggcta cgagctgatc
     3181 atcgttcatg gcaacggccc tcaggtcggc aacctgctca tccagcagga ggcggcggcg
     3241 gaccacatcc ccccctattc cctcgatctc tgtgacgcca tgaccgaagg cagcatgggc
     3301 tacatgctgg aacgggccct ggtcaacgag ttccggcgcc attcgatcga taaggaagcc
     3361 acgaccgtgg tgacgatggt cttggtcgac aagaacgacc cggccttcgc caagccgacc
     3421 aaaccggtcg ggccgttcta cacggccgaa cgggcccagg gattgaagaa ggaaaagggc
     3481 tggacgatgg tcgaggacgc cggccgcggc tggcgcaagg tcgtggcttc gcccaagccg
     3541 atcgacatcg tccccaagtg gattatcaac gacctcgtcc aggcgggccg gatcgtcatc
     3601 gccgccggag gcggaggcat ccccgtcatc ctcaacggca acggcctgta cgagggcatc
     3661 gaggcggtta tcgataagga ttacgcctcg agcctcatcg cccgggaaat cggggtcgac
     3721 ctgttcatca tcctgacggc catcgaccgc gtctgcctcg acttcggcaa gccggggcag
     3781 cgcgatgtgg cggttttgtc cgtctcggaa gcccgccggt ttttgtccga aggccagttt
     3841 cccccgggct caatggggcc gaaaatccgc gcggccatcg aatatatcga ggcggggggc
     3901 aaggaagtcc tcatcaccga cgccgcccac ctgaaggcgg ctttgctcgg ccggtccgga
     3961 acccggatcg tcagagacga ccacaaaaca gaaggagcct gatatgaaga aagatttcat
     4021 ctccatccac gacctcaccc ggtacgagtt cttcgaactg atggaccaga ctaaaaaaat
     4081 caaagacgat ccggacgaat accgaaagaa gctgaaaaac caggttctgg cgatgatctt
     4141 cgagaaaccg tcgctgcgga cccggatgac cttcgaagtc ggaatgctcg aactgggagg
     4201 aaaagccatc tacctcagcc ccagcgagat ccagatcggc aagcgcgaga ccgtcgagga
     4261 tgtcgcccac aacctcgagc gctgggtcga cgggatcatg atccggacgt tcggccacga
     4321 caatgtcatc cgcctggccc aggccgcctc cgtgcccgtc atcaacgctc tgaccgacct
     4381 cctccatccc tgccaagcca tggccgattt cttcaccctg aaggaacaca agggcaacat
     4441 ctccaagatc aagctcgcct gggtcggcga cggcaacaac gtctgccaca gcctgatgtt
     4501 cgccgcggct aaggccggct gcaaaatgag cgcggccgtt cccaagggct atgagccgaa
     4561 tcccgagatc gtccggctgg ccctcgagga cggaaaggga tcggatttcg agctgatcgt
     4621 gaccaacgat cccctcgagg ccgtggccgg agccgacgcc atctacgccg acgtctggac
     4681 gtcgatgggc caggaagccg agaaagagga gcgcttgaag ctcttcgcct ccttccaagt
     4741 caacgaagcc ctgatgtcca aggccaagga cgactgcgtc ttcatgcatt gcctcccggc
     4801 ccatcgcggg gaggaagtca cggctgaagt cttcgacggg ccccggtcca tcgtctttga
     4861 tgaggccgaa aaccgccttc acgcccagaa ggcgatcatg ctggccttga tgggcaaata
     4921 aacgagaacc tatatggaaa aggacaacgc tctcgtccag gtgaccccgg acgacatctc
     4981 cttcatcgag aaggagctgg ccggctcgcg gaaaccgacg acgaccaaag atctcgccgg
     5041 caagctggct taccagaaaa ccgccggcga gaggacccgg gccgtcaagc tctacgatcc
     5101 ggcctgctcc tacgaggtcg gggatacgat ctgcaaggaa tatgacgagg atctgatcgt
     5161 cggggcgaag gtccacgaac actttcaggg ggccgttgtc ctgaccgtca tccgcaagtt
     5221 cttttacaag gacttcgact gcgagatgct cgaggtcgat tacaccggag ggggcacctt
     5281 ccgcaagcac atcgactaca tgaaaaagtc caagatccaa gtgatgctcc cggccaacga
     5341 gggcgggaca aaccgcccgc cccggatcat gccccaggcc gaggatcctc ggctgaccga
     5401 gctgccgatg accgagaggg acctcaaaac cctggaacgg ctgctgcggg cggccctggc
     5461 caagtctccg gcttttttcg cctggggcga ccggtggcag ctgacggccg cccagccctc
     5521 cctgccggaa gaaacgatca aggagatcga agcccacctg gccgagacca aggcttcggc
     5581 cgcgacggcc gagcttgtct cgacgttttt caggatggag ccgtcccacg atctcttcga
     5641 cctccattgc ctggccctca accgcctcct ggaaacaaaa taccggaggg atttcgtcct
     5701 ggttcagccg gtcgactggg gccggtggat gctcaagagc gttctttcgg ccatgccgga
     5761 gggcctgcct ctagcggctc ccatggccgc ccttccgccg tttgaaaacg aggaaacgat
     5821 cgccgtcact cccttccacg agtttccgct gaaagtctac ctcggctggc gggaaatcct
     5881 ctccggcggc gtcaagatcc ccaagggatt caacaaagag ctgtcccaag cccgggaata
     5941 tgttttcacc gacgccgacg aaaagaaaag ctacaccgtc cattacttcc cccagcaggg
     6001 gtatttcctg gggctgcgcg aatatttcga aacaacggcg atcccccagg gcacaagcat
     6061 gaccctggaa aaaaccggtc cgatccagtt caacttctgg ctgaaaaagt cgaagaagaa
     6121 gatccaagtc gcccgcatgg cctatgaaac caaaggcgac accttcgccg aagcgggcga
     6181 aatggccacc ctggccatgc cgaacaagat catctatctc gaacgcgaac acctcgagcg
     6241 gctgacggcc ctgatcccgc agcggacggg gttggacctc aagggtctcc tcatcctggt
     6301 tttcaagcag ttcagcctcc aatcggcgaa ttattccctc cattacctcc gggcctatca
     6361 tcttgtcgac attctccggc ggaccaccca ggaggacgtg gaattcaccc ttctcaacag
     6421 tccggaattt gaaaaatcag acaagaagaa ggggattttc tactaccatg aaaccccttc
     6481 ggagacgcct gtcgaggaag cggagggaga agaagccgcg gccgaggaaa tcatttccga
     6541 agaggccggc gagctgacct cggatgactt cctggaacac gagttcacga ttctcgagca
     6601 agccccgccg ccggcgccgc cggccgcggc tcggaaagag gaagaagccg cagcgatggc
     6661 cgaagcggag gccgagctcg aggcgttcga ggcggaaaag aaagtcaaaa tcgagaagga
     6721 agcggccccc cctcctaaaa aagaaaaaga gaagaagaaa atcggcctcg aaagcgaccg
     6781 ccggccgaga tcccgcaaaa gcgaacggcg ggtcaaggaa gaggagctcg tggagctcga
     6841 gtccgtgcgc gaggcgttgt tcgccgaaaa aggcgcggaa agcgaggggc tggagcttcc
     6901 cgccgataag acggaattcg aacagcccga agtcccggtt gaaacggctc cgccggccgg
     6961 gcccggcccg cttggcgcgt tcggcggcgg cctgttcgcc gagaagctca aggtcgctct
     7021 gaagaagaaa agaggggaaa aggccgaaga gccggacaaa ccggacgaaa cgtcctgaac
     7081 cgcttcccgt ttcgagtttc atgggcaggc agagaaaggc cggcgtgatc ggccgattca
     7141 cggtcgccgc gcctctcgct ttttttctcg ttagcgttcc gagcagcctt ctcggccggg
     7201 aagaaacgtc tcggccggcc ggccggttcc gggccgcgtt cggcctggat tccctggaac
     7261 gcaaatatta ccagccggag ttccgcttgt ccgcgccgct cggcggtccc ggcgggaacg
     7321 ggctttttct cgacctgagc tacctccaaa aaatcaacgg cgacatggaa ggcccgatcg
     7381 acttctgggt tcgggccggc tgggccgccc ccctgtcggc cggagtcgag gccgaggcga
     7441 gcttgaatca tttctgccgg caccagacgt cgctgttcag cgattatatt ctgaacctca
     7501 acgagattgt cgggcgcctc cggcttcgac acggcggatt ccgggccggc ttcggcttgg
     7561 ggacgtatct cggaggaacc cctggctatg acggcttggc ggttttcgag gccgccggga
     7621 cgggactcct tttcccggag atttcctggg cggccgaggt caaatgggtg aatttctccg
     7681 agatcctcta cgaggcggag ctggccgtcg ccctttcccc aggcgcggag ctggtgctgg
     7741 ccggcttaaa gacctatcgg ctcccggcgg cgactcacgt cggcgttcgt ttcggagccg
     7801 acggcggagg ctcccggttt ctcgacgcct ttgacgtaac ggccggagtc tatccctttt
     7861 acgacgccta caaggtgatg atcgacggga ccttccgcct gccgcttctc cgccgggaat
     7921 cccggcgctt tttcattgac accgccttca gttcgcccct tctgaccggt tccggtttct
     7981 ggggccaatt ctggccggac cggatgatgt actccgtcac ggccgaatac gagcggccgg
     8041 tcgggagggc gttcgcctcc gtctacgggc gttacatcgc cgatatgccg gtggataaag
     8101 ccgttcgatt ccgggccggc gcttccaccg gtctggccat ccgaaaccag cccgatttca
     8161 accgccttga cggtccgatc cgcttcgagg cccgggcggg cttcgatttc aagttcgcct
     8221 atgacttcgg gatgaagatc ggcctcaaca cgaccggccc ggcggacctg aaagccggcc
     8281 tcgatgtcct cttcgaggcc aacgacgccc gccgcaccgt tgaagcccgg gttttcctcg
     8341 atttcggccg ggacgtgtcc gtccggccgt ttgtcggcgt gcggcgcgtc gtccccctgg
     8401 ccggagactc cgcttccgac ggaacgttcc cgcggatcgt ggccgcgggg atttctttct
     8461 acacctggtt tgactaggac ctcgaagacg gcgacgaggg ctcttccaga atctcgcggc
     8521 cggcgccctt tcttcgggtg acgcccattt cgtcaagcag ctccaagagg ggaatcgcgt
     8581 atttccggct gaggcccgtc atctccttga attccgcaac cggcaggtcc cgcggtcctt
     8641 tggcccgaag cttttcgatc agatcctcca gccacggccg gtggacgtaa tacccgtctt
     8701 ttccggcgat gatccgtttc cgctcgacca gaatgtccaa caactggttc cgggtttcag
     8761 gcgaaagatt catccgttcg cagacttcag ccggggaaac cgagcgggga cggccggcga
     8821 agcattcctc ttcgatcttt tggaggatgc gttcttcgcg ctcaggcagc tcccggacga
     8881 aatcggccag ggcgtagaat ccgtggtcct cccggagctt gccctcatgg acgagggttt
     8941 tcaggcacag aagcaaaatc tggcgcggga tttcgaagcg ctcctggatt ttggaaaggg
     9001 cgacgccgcg ctccttgatg tgcgaggaat ggaatttctc gagataggcg acgaatttct
     9061 cctcgaaata atcgatcgcc tcccgggcga caagatggag aggggcgaag gagaggatgc
     9121 ggatccggcc ttcggactcc agcgccttgg ccagatcggt caatccctgg aaatccgggg
     9181 ggaaaacgcc ggctaaatcc ttttgccgga gcccctgcaa tccataagcc ttggccaggg
     9241 cgatgatctt ggggccgtcg ccggccggaa gacgcttggg aatcggaggg tgggaggcgc
     9301 gcatggtgac tgttctcgaa accgcgtttc attgtaaggg aaacgagaaa gaaagaaaag
     9361 aaacaggcct ccccgggagt ccgtccgttt cagcctcgac gaacgccctt gatggctgaa
     9421 gaggaaatcc tttcaatgaa agacggaatt caatgtccgc gcttttttcg gccgcggctt
     9481 gtttttcgtt cgaggccggc tggctcttgc tccgccggca acaccaggct catccgccgg
     9541 ttgacgggat cgacggcggc gatctggacg gaaaggccct ggccgagctc gtattgccgg
     9601 ccggtgcgct tgccggtcag cgcgccgcgg gagcgcttgg cgaaataatc cccgcccagg
     9661 tcgtcgtagg cgagcaaccc ggaaacgaaa tactcgtcaa gctcgacgat cagcccgccg
     9721 cgggaaaagt cgacgatcag accggagacg acgtcgccga ggcgttcctt gagaaaccgg
     9781 aagatccgcc attcgacgag gtccttctcg gccgcgtcgg ccttgcgttc ctgttcggac
     9841 gaatgaacgg cggccgcctc gagcccggcc gggcgaggga cgcgtccggc caggtcgtcc
     9901 ttcagaagcc ggtggacgac gagatccggg tagcggcgga tcggggaggt gaaatgggtg
     9961 taatccttga gcccgaggcc gaagtgcccg gatcgttccg aggaatattc ggccagccgc
    10021 atcgcccgga ggatctggac gctgacgaac ttctcgagcc gcgttccctc ggccgcccgg
    10081 acggcggatt gtagatcccg ggaagtgatc ctcttggatt cggggagcag gatgtggaag
    10141 aaggccagcg ttgcccggag cttctcgagg tcctcctcgg ccggggcctc gtggacgcgg
    10201 tggagggagg ggatcccgcg gaggcggaga tattcggcca cggcgacgtt ggccgcgacc
    10261 atgaattcct cgatgagctc atgggcttcg ttccgggcca aagccgcgac cgaggcgact
    10321 ttcccttcct ggtagacaag ctccggctcg accaggtcga agtccaggct cccccccgcc
    10381 agccgccgct tccggatcgc ccgggccagc gttcgcatgt ccaggatgtc ggggacgatc
    10441 gaggcgcggg cctcccgctc ggccggatcg ttttccatga tcttgaagac cgaatcgtac
    10501 gtcatccgtt cgaccgtccg gatgaccgag ggatggaagg acgtcccgat ccgctccccc
    10561 tccgcattga aatccatgac gacggaaacg gccaagcgct cgaggcgcgg ccgaagcgag
    10621 cagagatcgt tggagagccg ggccgggagc atcggcaggg tcaggccggg aaagtaaacg
    10681 cttgtcgccc gggcgaaggc ctcggcctcc agggccgtcc ccggccggac gtaatgcgac
    10741 acgtcggcga tgtgaacccc gagccggaag ccgccggacg gcctccggga aatggagacg
    10801 gcgtcgtcga agtcctgggc ggcctcgccg tcgatcgtga acgttttcca tgagcggaaa
    10861 tcggcccggc cggcgatcgt ctcggccgcg gagacggcgg cggtttgctc cgcttccgcc
    10921 tcggccgccg gggaaaaagc atccgccagt ccgtactttc ggatgatgac ttgggcgtcg
    10981 acgccgggat cgtccggccg gccgagcacc tcgacgattt tcatgtccgc ccgctcgacg
    11041 gaaacgatct ccccggcttc cgggaaaaac gatccccggg aaacgatcgg gatttcctcg
    11101 gccgacgggg tttcgaacgg gacgaaaaac gggcggccga accgctcacc gaagatcccc
    11161 aggagccgct ttttgacttt cttgacgatc cggacgaccc ggccttccag ccgcccgctc
    11221 cggccggacg cgttcacgat cacctcgacc tggtcgccgt ggaccgcccc ggaggaaaac
    11281 cgggatggaa caaagacatc ctcccccttt tcggagacga cgaagccgaa gccgcggccg
    11341 aaggtttcaa acgttccttt ccgcaggtcg gaggccggcg gcaggagcca gcgatccctc
    11401 gcccgacggg ccagcttgcg atgaacgaga ctctgaaggc ggtcctcgac cttggcccgg
    11461 gatttgcgat cgacccgcag ctccttgaag atatcccgga gcttcaggcc ctggggattc
    11521 cggcggagaa ggttgagaat ccgggcttca tcgctttgcg ctttggccat ggggggcgat
    11581 cctttcaccg gcggaggccg ccggccccgg ccgggccggg ccgcatccgc tcaataataa
    11641 tagagcgaca catagggaat ccggtgtttc tcccggagat ttcggaccag ccggatgcgg
    11701 gcggcggatt ccttctccgg gccgcgaaga ccggggtggt cgcggtcgac ggccgcttcg
    11761 gcgtcgccga gacgggcggg cccggccgag tcccggatcc aggcgtcgat cttcccgtcc
    11821 agccgctcca gggcctcgtc gtccggacgc ttcgccttca agagcgaccg agccttctcg
    11881 aattcgggca ggagcgcggc aaatccggcc ggccgccggc cgaggaattc atccacggcc
    11941 gcccgggctt tttcgatttt ctccgttgat ctcgtttcca actcgggact ccgtcgtccc
    12001 acgtcccgct cgcggctccg ctcgagggcc cgctcgacgg ccgcccggca gaaggacaac
    12061 gtccggatct tccccggcgc acggccccgg ccggggcgga gggcaaacgc cgcctccagg
    12121 ccctccaaga ccgtttcgag ggggatcccg gcggattccc aggcttcgat caagcagatg
    12181 tcggccgggg aaagcgaaaa cggcgctccc cgcagggcga tgaaacggtc ggcgatgccg
    12241 cggaaatacc gctcccggaa tccggctgcg ctcggctcgt cgctcatccc tcgatcacga
    12301 ccttcacggc tcggacggcc ggtccttctg ccgttcgaag atgatccgaa gcccttccag
    12361 gacgagattc ggctcgccgg cgtcgatggc cggaacttca gccgcgattt cttcgccgaa
    12421 tccgccggtg gccacgactt ttgttttctc cccgagctct ttccgcagcc cgtcgatgat
    12481 agtctgcgtc aggccgatgt agccgtaata aaggcccgac tgcatgctgc ccacggtcgt
    12541 tttcccgatg atccggtccg gcttgcggat ttcaatccgg ggaagccggg ccgtcttgag
    12601 atagagggct tcggccgaga tcaggattcc gggagcgatc gccccgccca ggtattcgcc
    12661 cttggccgag acggcgtcga atgttgtagc cgtgccgaaa tcgacgacga tgcacggacc
    12721 gccgtatttc tcgtaggcgg cgacggccgc cgagatgcgg tccgcgccga cctcggccgg
    12781 ggcatcgtag aggatgggca tgcccgtgcg cagccccggc ccgacaacga acggcctgac
    12841 gccgaacaac gacccgcaga gcttctggaa aaccggggtc aggggcggca caacgcacga
    12901 aatgatcgcc gccttgacgg cggccggatc gatcccggcc atgcgaagga gattgaggag
    12961 gatgacgccg tattcgtcgc tggtttgatc ccgatccgac cggagacgcc attcccggac
    13021 gagcttccgg ctttcgaaaa cgccgaaagc caccgaggtg ttgccaatat cgcaggccaa
    13081 tagcattgtt tttctcctcg cccggatccg ggggttccgg acctgatttg aaggcggtcc
    13141 tttttccgat tatactccaa atgactcgcc ggaaggggcc cggtcgaggc cgaacgaaac
    13201 gatttcggcc gagacaaacc gcctcgtccc cccggccgtt ctcacccgga tggccccgtc
    13261 gatatccagc ccctcgaaca atccggcaac gtcccccgac gccgttcgaa gccggatcgc
    13321 ttcccccggc ccggcttcgg cgtggagaag aaaggcgtcc accacgtcct ccggccgctt
    13381 ttcgaagaga ggcgcgaccg ctccctcgag ctcctccagg aacgcggcga ggacgtcttc
    13441 ccggtcgatg attctccccg cggccaggcg gagggatccg gcccgggccc gccattcctc
    13501 cgggaaatcg ctttcgtctt gactgaggtt aaggccgagg ccgacgatcg cgtagcgcga
    13561 acgaaatccg gacaatccgc tttcgcagag gattccgccg agcttcctcc cttgccagac
    13621 gatgtcgttc ggccagcgca ctttcgccgg aatccccgcc gtccgctcca cggaccggcg
    13681 ggcggccagg ccggcggcca ggggcagaag cgaaagcgcg gcgtccgtcc cgggacgcag
    13741 gacgacggag acatacaatc ccgccccccg gggggaaaac caggagcggc ccttggtccc
    13801 ccgtcctccg gtctgttccc gggcgacgac gaccgtcccg gccggagcgc cctcgcgagc
    13861 gcgtttccgg gcctcatcat tcgtcgaaac acacgtgtcc aggaagcaga ccgagtcatt
    13921 catggggatg cgttcaccgc ttgccgtcga cggccttgag ctcctcgacc aggaccttct
    13981 tgaactcggc cgagacgatg tcccgggccc gttcctccat ttcggcctgg ttgcgaagaa
    14041 tttcctgccg gatcacgtcc cggattttcc gctcgacgtc gtcggtccac tcaggcaggt
    14101 tccccgagtc cggagtgacc gggccggatg cgaccgaggc ggtctcggga agactctgcg
    14161 gctcgagaaa gggatcctcg ggaagcgagg gcagctcttt tttgcgatcg atcgccgtcc
    14221 ggatccggct caaaagggtt tccccgtcaa acggcttccg gatcaagccg tcgtgccgga
    14281 tgacggacag cttggccacg tcgacggcct cgaaggcgcc cggcagaagg aaaagggccg
    14341 tcgtccggta gaccggttgg gaacagagaa aggcggccac atcgtatccg tccatgccgg
    14401 ggagggaaag agcgacaagg acggcgtcgg ggaagaattc gggaaggatc cggatcgcgg
    14461 ccaggccgtc gggcgcggtc cggacttcaa attccgctcc gggcagggcc gtttccacgg
    14521 ccttgcggac cacgggggaa gcgtcggcca ggaggatttt catgctcatt tcaataatca
    14581 acccggatcg aatccgggcc caggagcgtt tcaagagaag tcagcaggtc gtccgtgggc
    14641 gtcacgcctt tcagctcggg gctttggagg accacccggt gacccgacgg cgtttccaat
    14701 tcgaacacgg ccgggcattc gccgggcgat ttctccagca cttcccgcat gtcttggaga
    14761 acggcctcgt tcagggggcg ggcgggcagc cggacgacga acttcttggc cagcttctgg
    14821 gcggcctcct ccaggggcag gatgtgggac aggtaaatct tgtgggtttc gccgtcgagc
    14881 tggtaatggc ccttgatcca gacgaggctt ccctcctgga gaacatggta atagcgcttg
    14941 tagggttcgg cgtagacgac gacctccacc cgcccggtca agtcctccag ctggaagacg
    15001 gccatccgtt cgtccttctt cgttttcttc atctggaaat cggcgatcac gccggccacc
    15061 cggatctcct cggggatatc ggcttcctct tcgaaagacg ccgtcgagtg ggacgtcaga
    15121 cgggggagga tgcgccggta atgggaaagg ggatggccgc tgatgtagaa gcccaaggct
    15181 tccttttcgt aggaccactt gagcatctcg tcccactccg aaatcgcggc gatttcggcc
    15241 ggaatctccg gaggggcgag cgcgtctctt ccgaacaaca gggattgggt tgattcccgg
    15301 attttctgca tctcccggtt gtagtcgatc aggttgtcga tcaggcggaa gcttcgggcc
    15361 cgggggagtc cgaacgaatc gaaagcgccc gccttgatca ggctctccag gaccttccgg
    15421 ttgatgatcc gcccgtcggc gtggccgacg acgtcgaaaa tcgtctggaa cgggccgcgc
    15481 tccctccgaa cggcgatcag cgcccgggcg gccgtctcgc cgacgttctt gaccgcggcc
    15541 aggccgaagc ggacggcccg gtcctcgacg atgaaatcga aatcgctctt gttgatgtcc
    15601 ggcggccgga cctcgaggcc gaggacctgg cactcggcga cgtacttgac gatctgggag
    15661 gtcgccccgc gctccgcctc ggacgtcatc agcgcggcca tgaaatgcgt cgggtgatgc
    15721 gccttgagat aggccgtttg ataggccagg gcggcatagg cggcactgtg cgatttgttg
    15781 aatccgtatt cggcgaactt ggcgatctgg tcaaagattt tcgcggcttt cgaggccgaa
    15841 atggcgccct tccgtttcgc cccgtccagg aaattctgct tctgggcggt catgatctcg
    15901 gccttcttct tgcccatggc cttgcgcaga agatcggcct tgctcaaaga aaaaccggcc
    15961 agctcgaccg cgatccgcat cacctgctcc tggtagatga tcaggccgcg ggtttcctcg
    16021 agaatcggct tgagctcggg aatgtcatag acaatcttct cgggatggaa tttccgctcg
    16081 atgaagtcat tggccatccc gctcttcagg ggccccggcc ggtagagggc attcagggcg
    16141 atcaagtcgc ggaactcctc cggcttgtag ctccgcagga gcgctttcat cccttgggat
    16201 tcgaactgga agaccccgtc ggtgttgccg gccttgaaaa gatcgaatgt cggcccgtca
    16261 tccaggggca gggcgtccac gtccggggcc gtcccgaagt ccttggcgat caggttctgg
    16321 gcgtcgcgga taacggtcag gttgcggagg cccaggagat ccattttgag gaggccgatc
    16381 gcctcgattt cgctcatggc gaactgggtc gtcacctcgc ccttgaccga ctggtacaag
    16441 ggaagaaatt cagtcagcgg ccgcggggtg atgaccaggc cggcggcgtg aatcgacggg
    16501 tggcggacct ggccctcgac cttctcggcg atgtccagaa gggctttgat ccgaggattc
    16561 ctctcgacgt attcctggag cgggcccgcg tcctggacgg ccgaggcgat cttgacgtcc
    16621 ggaccgagag gaaccatctt ggccacttta tcgacttcgc tcagggggac ttccaggacg
    16681 cgtcccacgt ctcggatggc cgcccgggcg gccatcgtcc cgaaggtgat gatctggctg
    16741 acgttctcct gcccgtattt ctcggtgaca taggaaatca tttccgcccg gcggcgggcg
    16801 cagaagtcca tatcgatgtc cggcaggctg attcgttcag gattgagaaa ccgctcgaaa
    16861 agaagatcgt actcgagagg gtcgatgtcg gtgatctgaa ggcagtaggc gagcagcgag
    16921 ccggcggccg agccgcgccc cggaccgacc ggaatgttcc gggaccgggc cgtccggaga
    16981 aggtcccaga cgatgaggaa atatccttcg aactgcatct gcttgaccag ctggatttcc
    17041 ctctccagac gatccttgta ggcgtcaagg tcatggacaa gcttgccctt ggcccgcaga
    17101 tcgcggagtt tgtcggcccg ctcggaaaat cccgcccaca cgacttcctc aaagtaatcc
    17161 cggagcgact tcccttccgg cggctcgaac cgaggcagga aatacgttcc cttgggaaat
    17221 tcgaaatcgc accgggcggc gacgtgaaaa gtgttgtcga gggcctcggg gatttcgccg
    17281 aacagccgct ccatctccgc cggcggcttg aaatagaact ccgccgagcc gaacttgatc
    17341 cggtcggtat cgctgatctt cttattcgtc tggatgcaaa gaaggacgtc gtggctggcc
    17401 gcgtcggctt tgccgaggaa atggatgtcg ttggtggcga caagcggcag gccgagccgg
    17461 cgggccaggc cgacgagctt gggattgacc tcccgctgcg gctcgaggcc gtgatccatg
    17521 agctcgatat agaaattccc cggcccgaag atctcggcat agctttcggc cgcccgaaga
    17581 gccgcctcct cccggccttg accgaagaga taggcgatct cgccgcgcag gcaggcggaa
    17641 aacccgatca gtcccttgct gtgggcggcc aggagctcct tgtcgatccg gggacggtaa
    17701 tagaatccct ccaagtaggc cttggacaac aggacgcaga ggttcttgta gccggtctcg
    17761 ttctcgacca ggagcaagag atgatagtgg gccggctcgc cgtcgcggag ctcgcggtca
    17821 cggcggctcc gcggggcgat ataggcctcg cagccgaaaa tcggcttgac gcctttgtct
    17881 ttggcgattc tctggaactt gaccgctccg aagaggttgc cgtgatcggt cagggccacg
    17941 gccggcatct tgtggtcgac ggcggcctgg accaaggcgt ccaggcggac ggcgccgtcc
    18001 aggatgctgt attccgagtg gttgtgaaga tggatgaagc cgggggccat ggattcattc
    18061 ccattcgatc gtgccgggcg gcttgctggt gacgtcgaag accacccggt tgactccggc
    18121 cacctcgttg acgatccggt tcgagatccg ggccagaagg ccgggggacg ccgggaacca
    18181 gtcggccgtc atcccgtcca cgctttggac cagccggatg acgacgaccc gctgataagt
    18241 ccgccgatca cccatgactc cgacgcttcg gacgggcagc agcacggcga aggcctgcca
    18301 caggcggtcg tagatcccgg ccgcccggac ttccctaacg acgatgtcgt cggcctcgcg
    18361 gacgatccgc aaggattcct cgttgaccgg gccgacgatc cggacggcca ggccggggcc
    18421 cgggaacgga tgctgggcga cgaaggcccg gtcggctccc agctccacgg ccacggcccg
    18481 gacttcgtcc ttgaaaagct cccggagagg ctcgaccagg acgaagccgt gcgatttcgg
    18541 caggccgccg acgttgtgat gggacttgat gacggccgag ggcccctgga cgggacggct
    18601 ctcgatcacg tcgggataaa tcgtcccctg ggccaagaac ttcgccccgc ccaggcggcg
    18661 ggcctcctcc cggaagacct tgatgaacaa gccgccgatg atcttccttt tccgctccgg
    18721 gtcggagacg ccgcccagtg ctttgacgaa ggccgcggcc gcgtccacgc cgacgacgtc
    18781 caagtcgagc ttgacccgga acatctccat gaggccggcg tactggccct cccggagcag
    18841 gccgttgtcg acgaagacgc aagtcaggcg ttttccgacg gcccggtgaa ccaggagggc
    18901 cgtcaccagc gaatccacgc cgcccgaaag cccgcagaga acgcggcccc ggccgaccgt
    18961 cttccggatc tcctcggtct tgcgctcgat aaacgatccc atattccagt cggggatcag
    19021 gccggcgatc ttgaagagga aattagctag aagcttccgg ccttccttgg tgtggatgac
    19081 ctcgggatgg aactggacgc cgtagaaatc gcgccgcggg tcttcgatca ctccggcccg
    19141 cgtgttcccc gtccggccgg agacgcggaa gcccggcggg agcttttcga ccttgtcgcc
    19201 gtggctcatc caaacctgtt cgccgtcttt gaggccggac agcagcccgc tccggcggac
    19261 gagacggaga gcggcgaagc cgtattcacg ccggggagac gggacgaccc ggccgcccag
    19321 aagatgggcc atgagctgca gtccgtaaca gatgccgagg ataggaacgc cgagggtgaa
    19381 gatctccggg gagatcgtcg gagccccttt cccataaacg ctttgcggtc cgcccgaaag
    19441 gataagcccc tgcggccggg cggcccggat cgcctcgacc ggggccgtcg gaggccagat
    19501 ttcggagtac acgcccagct cccgcacctt gcgggcgatg agctgggtat actgcgaccc
    19561 gaaatcaatc accaggattt tattcacgaa gcgattgtat cacccgcggc ccggggcctc
    19621 aagggcgaaa gcggcgggtt cgcggcatgt ccgagaacgg agtccgcgcg cccctcccgg
    19681 ggaacgcggt atgctataat atcgtttcca tttcattcgg agagtcgcca tgagctttga
    19741 agaactgtcc cgcgagctcg cggttcaaac cgacacgaag atcgttctct tcgtcatcga
    19801 cggcctgggc gggctgccgc gaaacggacg gaccgaactg gaaacggcgg ccgcgccgaa
    19861 cctcgaccgc ctggccgccc ggtccagctg cgggctcacc gatccggtcc tcatgggcgt
    19921 gacgccgggc agcggcccgg cccacttggc ccttttcggc tacgatcccc tccgccaccg
    19981 gctcggccgg ggaatcctcg aggccctggg ctcggatgtc gaggtccggg aaggcgacct
    20041 cgtcgcccgg ggaaacttcg ccactctcaa ggacggcttg atcgccgacc ggcgggccgg
    20101 acggattccg acgagcgagt gcgaacggat ctgcgccaaa atcaacgccg cccgggccgg
    20161 aatgtccaat cctgaaatca ctctcttcgc cggcaaggaa caccgcttcg tcgcccggtt
    20221 ccgggctccc gggctctccg acgccttgac cgacgccgat ccgcagaaag acggcaagcc
    20281 ccgcgtcccg gcgtccgcgc tggacgcggc ctcggagaaa gcggcttcca tcgtcaacac
    20341 tttccttgac gagattctag ggatcctcaa ggacgagccg gcagccaacg ccgccctgct
    20401 tcgcggcttc tcggttcagc cgaccatccc gaccatggcc gacctgttca aactccggcc
    20461 ggcggcgatc gccaactatc cgatgtacaa gggtttggcc cggctgatcg gaatgcatgt
    20521 ttataaagtc ggccccgagc cggaggacct cttctccgct ctcgaggccc gctggaaaga
    20581 ccacgacttt ttctacgttc actttaaaaa aacggacgcg gccggggaag acgggaactt
    20641 cgaagccaag gtccgggcga tcgaggaggc cgaccgcttc cttccccgga tcgaagccct
    20701 caaacccgac gttctcgtcg tcaccgccga ccactcgacc ccggccttga tgcgcgggca
    20761 ttccttccac ccgaatccgt tcctcctggc cgccccgacc gccttgcccg acgatgtcgt
    20821 cgccttctca gagcgggcct gcgccaaagg catcctcggc cgctttccct cgcttcgggc
    20881 catgccgctg attctcggcc atgccgggaa gttgaaaaaa tacggcgcct gattcggagg
    20941 ccttccccgg acgagccggc ggtccttccg ccggcccgtc tttttcggca aatcaaccgt
    21001 gttataatat ttctcgaaca aaacggatga gtggacgaag aggccgtctc tggacgattt
    21061 tcccggtcct gattctctcc ttagccgccg ccccggccga aaaaacggac gttcccgtcc
    21121 tctccgaagt ctataacgtc cggtcctacg cccatcccaa ctacacccgc atcgtcctgg
    21181 acgtgggcgc tccgcgggaa tacgtctgga acgagaccct cacgccgccg gagatcaccg
    21241 tcgacgtcat ccgggcccgg cttaatccca tcgttcccga aaccgtcacc ccggccggcg
    21301 ccggctacat ccggcgtctc cgtctcgcgg caaagaatcc gacgaccgtg cgcctgacga
    21361 ttgaggccga ctgggaccgc gtccgccggt atgacgtctt caacctcttt gatccgttcc
    21421 ggatcgtcat cgacatttat ccgaaagacc ccgcggccgg cccggcggcg gccgccccgc
    21481 cggctgaaaa aatcccgaag cccgcccagc cggcgggggg cggatacagc ctcgcccgcc
    21541 agctcgggct gggcgtgcgg acgatcatca tcgatcccgg gcacggcgga tcggatccgg
    21601 gctgtctcga tccggccggt ccgaaggaaa aggacctgac cctcgacatc gccctcaagc
    21661 tcaaagcgat cctcgaagcc gagtccgatc tgtccgccgt cctgacgcgg gaaaccgaca
    21721 tcttcgtccc cttggaagcg cggaccatcg tcgccaatca acggaaagcc gacctgttca
    21781 tctccatcca cgtgaacgcc taccggctga aagcgcggca ggggctggag acgttcttcc
    21841 tgaatttcag ccccgatccc gcggtcaacg agctggcagc caaggaaaac gcgacgacga
    21901 caaaaaccat cggcgagatg gacaaaatcg tcaagaaagt cgtccaaaac agccggatca
    21961 tcgaatcccg ggagctggcc cggacggtcc aatccgccct ggcttcccat ctttcccgtc
    22021 actacgccgg cgtcaaggac ctgggcgcca aaggcggccc gttctgggtg cttctgggaa
    22081 gcgagatgcc ggcgatcctg atcgaagccg gacatctttc caatcccgag gagatcaagc
    22141 ggctccagag cgcggtgtac cgccagaata tcgcccgggg aattttcgag gggatcaaag
    22201 cctacatccg ttccctcggt aaaggataaa cccatgaaac gaagaacgtt tctcaagagc
    22261 gccgtctggg gcggcgcggc tctcagcgcc gtccccggcc tgatcggccg gccggccgcg
    22321 gcccccggcc cgcccgacct ggccgtcatc cagggaaatt cgccggcgga catcgcccgg
    22381 gcggccgtcg aggccctcgg cgggatgaag cggtttgtct cccgggggga cgtcgttctc
    22441 atcaagccga acatcggctg ggaccgcacc ccggagatgg ccgcctgcac caacccggac
    22501 atcgtccgga ccctggtcga gctcagcttc gccgccgggg cgaaaaaggt cgcggtgatg
    22561 gacaacacca ccaatcaagc ccagcgctgc tatgtccgct cgggcatcca ggcggcggcc
    22621 agggaagccg gggccgaggt gcccttcatc aacccgaggc ggatcaagaa aatgaccatc
    22681 ggcggcgcct ggctcaagga atgggacgtg atccaggact tcgtcgaatg cgacgccctc
    22741 atcaacgttc cgatcgccaa gcaccacaac ctctgccggg tgacgctggg gatgaagaac
    22801 tggctggggg cgatcggcga cccgcgcaac cgcctccacc agcgcatcga cgaggcctcg
    22861 gccgatttgg ccgccttctt caagcccaag ctgaccgttc tcgacgcctg gcggatcctc
    22921 atccgcaacg gcccccaggg cggccggccg tcggacacca agctcttgaa gacggccgtg
    22981 gccggaacgg acgcggccgc ggtcgaagcc atgggagccg ttcttttcga tatcgtcccc
    23041 gccgagctcc ctctcctgcg cttcgccgtt gaaagaaaca tcggccggat cgatctggag
    23101 aagatgaaca ttgaaaaaag aacgctctaa gaaattccgc cgcctccaga tcgcccgaat
    23161 cgccgtccag acggcgttcc tcgggctgtt tttcctcctc ctcttcggcg cgcatttcac
    23221 cggagaggat tacatcggcc gggtcgaggc tttttttcat ttcgaccctc tcctggccgc
    23281 ggccacgatc ctcgcctcac gcgtcttcct cgtttatttc gcctgggccg cccttacggt
    23341 cctcgtcacc gtcctgttcg gcaggatcgt ctgcggctgg atctgccccc tggggaccgt
    23401 ccaccagctc ttttcctgga ttttcaagaa agcgcggttc ctccggccga aaaagccggc
    23461 ctcgggcggg ttatggatca aatacgccgt ccttgccttc gtcctcgtcg ccgccctctt
    23521 cggcctcaac gccgcggggt acctggaccc gctgtcgttc ctgacgcgcg cctcggccgc
    23581 cggccttatc ccggccttcg gtctaggagc ggacgccctt ctcggggccg cctacgcggt
    23641 caggctcgag gccttcggcg acacgctggc ccaggctctc tcgactctga ccctgaacac
    23701 gaccttccgg cagggatttc tcctcggctt gatttttctc ggagccgttc ttctgaacaa
    23761 aatccgggag cgcttttggt gccggtatgt ctgccccctg ggggcgctcc tgggcctctt
    23821 cagccgatac aacctgttca agctgaaaat cgacgaatcg aagtgcaccc aatgcggcct
    23881 ctgctcgatc cactgccaaa cccaggccgc tcccttcccg gccgggggat ggaaaagctc
    23941 ggagtgcgtg tattgcgaga cctgcgcggc gatctgtccg gagcgggcga tcacgttccc
    24001 ggtccgcctc ggcgcggaga agccgccggc cgtcgccttg acccgccggc gtctgctggc
    24061 gacatcgttt ttggctttct tcagcgtccc gtttttccgg atcacgtccg cccggactcg
    24121 ggccgatgaa catctcatcc ggccgcccgg atcgctgccc gagccggcct tcctccggac
    24181 ctgcatcaag tgcggcgagt gtctgaaagc ctgcccgacc aacggtctcc agcccgccct
    24241 gaccgaagcc gggcccgagg gcctgtggac gccggtgctg gtcccgaaaa tcggctattg
    24301 cgagtactat tgttcgctct gcacccaggt ctgtccgacc ggggcgatca aggaactgac
    24361 cgtcccggaa aaaacgacgg tcaaaatcgg gacggcctgg atcgacaaga accgctgcct
    24421 cccctatttc ctcggacggc cctgcatcgt ctgcgaggag cattgccccg tctctcccaa
    24481 ggcgatcaag ttcatcctcg tcgagacgaa acggcccgac ggaacgacgg ccgtggaaaa
    24541 agctccgatc atcgatcccg agctgtgcat cggctgcggc gtttgcgaga acaagtgccc
    24601 ggtggccgac cggccggcga ttttcgtcac gagcgtcggg gaacgccggt cgaaggaaaa
    24661 tcagctgctc ttgaacgtcg gcgagtaagc ggatccggac cggccgcggc cgacgacgat
    24721 tgaagtcccg gcccatctgt actagattgg gagaccgccg ccggggaaac cctcgagaca
    24781 tcccggcggc gcgccaaacc gcgctcacgg aggatacgcc gccatgaaac aaagaaccgc
    24841 cgccttcatc ttgacgcttg cgcttttcgt tccgtccatc gccgcgcccg gcccccggcc
    24901 cgccccggcc gccgaaacgg cggccgtcac ctcgccggaa acgtttttcg gattccggct
    24961 cggagccgac cggcggatgg cgccctggga caagatcgtc gcctactacc gcctcctcgc
    25021 ccgggaaagc gataagatca aggtcgtcga cctggggccg tcgacgatga accagccctt
    25081 ccttctggtc gtcatttcct cctcccgcaa tctcggccgc ctggaagagc tccgaaaaat
    25141 caacctccgc ctcagcgatc ctcgcgattt ggacgagacg gccgccgaaa agctgatcgc
    25201 cgaggggaag gccgtgatct gccagtccat gagtctccac gccaccgaaa tcggaggcac
    25261 ccagatggcc ccggagctgg cctatgatct cgtttcgcgg gccgacgagg agaccgcgcg
    25321 tatcttggac aacgtcgtct ttctcctggt tccgtccttc aatcccgacg gggcggtcat
    25381 ggtttacgat tggtaccaaa agaccctggg aacggaatac gagggatccg acctgccgtg
    25441 gctctaccac aaatacgccg gccacgacaa caaccgggac gcgtttcaga ccaacctggt
    25501 cgaatcccgc tacatggccc gcctcctgtt ccgcgattgg attccccaag cctatgtcga
    25561 ccaccaccac atgggcagct acggaccgcg gatctacgtg ccgccctacg ccgagcccat
    25621 ccgcccgctg gccgaccccc tgatctggcg ggagcacagc tggtacgggg cgcatatcgc
    25681 gtacaaggag gaagaagccg gattgtccgg cgtcatcaac gcggctcaat tctccggctg
    25741 gggtcatttc ggattccact ggatcacccc ctttcacaac atcgccggga tgctgaccga
    25801 atcggccgac gccaggctgg cctcgccgct ctatatccac ccccaccagc ttaagggcga
    25861 cgaccgcgga ttccccgctt acgaagccca gaccacgttc cccaatccct ggccgggcgg
    25921 ctggtggagg cttcgggaca tcatcgaccg ccagaaaatc tcggcttggg cggtgctcga
    25981 cctggccgcc cggaacaagg agaccgtcct gcggaacgcc tttctcaagg cccggagaca
    26041 gaccgagcgc ggcgcccagg gaaagcccgc cgcctacatc atcccggccg tccaacacga
    26101 tccgctgacg gctgtcaagc tggtcaacaa gctgctcgac cagggcatcg aaatccgccg
    26161 ggcggccgag gcgttcagag ccgccgacgg aaagccttat gccgccggat ccttcctggt
    26221 tcccctcgct cagcccaaaa tgggcctgat ccgctatctc ctcggccgga cgctctatcc
    26281 cgacaattcc ttcacccggc ggccggacgg aaccccggaa tccccttacg acatggccac
    26341 cgacacgatg gccgaattca tgggcgtccg ggtggaggct ctcgacgagg ccgtcaccgg
    26401 gaactttgaa acgctgaccg gggcgctcgc gccggccgga cgcgcagccg aaagcgccgc
    26461 cggccattcc ctcgacgggc gcctcaacga cgccttcgcg gcggccggac ggctgcttga
    26521 gcggggcatc cggctgcgtc gggccgaaga aagcgccggc ggccttcgtc ccggcgactt
    26581 ccttgttccg gccggaacgt caagcgccct gctttccgaa atcgcccgaa cgacgggcgt
    26641 cgacttccgg gccctcgagc cgggcctcga tcgaaccggt cgcgaggtca agcggcagcg
    26701 cctcggcgtg taccagcgct actggggcgg caacatggac gagggctgga cgcgcctcct
    26761 tctggaagag ttcgcgatcc cgtacacgac cctgagggac gcggagatca ataagggcgg
    26821 actgaacgcc gccttcgacg tcattctcct tccggacgac gcgccggctc tcatcaccgg
    26881 ggaaggattg gatcacaggc ccggactcct ggaatccacg cctccggaat accgcagcgg
    26941 aatcagggcc gaaggtttcg aagccttgaa gtcctttgtg gccaagggcg gcacgctggt
    27001 cgccttcggc cgcgcctgcg gcctggccgt ggagaaattc ggcctcccgg tccgcaacgt
    27061 cctggccgga atcaaccgca aggatttctg gtgcccggga tcgaccctgc gggtgacgta
    27121 cgatccaaag aatcccttgg cctacggtca gccggacgag gggctggtcg tgtttcagat
    27181 gggaaatccg gctttcgaga tccttccaac aaatcacaac gagcgggctg agatcgcggc
    27241 ggcctttgct tctcgcgatc ttctccaaag cggctggctt ttgggggaat cccgcctggc
    27301 cgggcgggcg gccgtcgttt cggtccggca cggcgagggc cgggtcgtcc ttttcggcat
    27361 ccggccccag caccgggccc agacgcacgg caccttcaag ctgttcttca acgccctgat
    27421 ccgctgacgg cggcggccgg cgggatcccg ccggaaatga tcaggagccg agggctttga
    27481 tttccgcgat caaggcgggc acgacctgaa acaggtcggc cacgattccg taatcggcca
    27541 ccttgaagat cggggcttcg ggatccttgt tgaccgcgac gaccaccttg gacgaggaca
    27601 tgccggccaa gtgctggatc gctccgctga tcccgaaggc aaaataaaga ttggggctga
    27661 cggttttgcc cgtttggccg acttggtggg aatggccgat ccagccggcg tcgacggccg
    27721 tccgggacgc cccgacggcc gcccgcggaa ggaccgccgc cagatcggaa agccgggcga
    27781 atccttccgg gcctttcatc gcccggccgc cggcgacgac gatatcggcc tcggccacat
    27841 cgatcgccgc cccggactcg ctcaagatct ccgcgacttt tccggcgacg gccccctccg
    27901 ggatcgcgac cgcgtggggg acaacctcgg cctcggggcc gggttccgcg tccagcggaa
    27961 aaacgttggg ccggagggtg gcgatcgccg gcccggcacg gcgagggccg ggtcgtcctt
    28021 ttcggcatcc ggccccagca ccgggcccag acgcacggca ccttcaagct gttcttcaac
    28081 gccctgatcc gctgacggcg gcggccggcg ggatcccgcc ggaaatgatc aggagccgag
    28141 ggctttgatt tccgcgatca aggcgggcac gacctgaaac aggtcggcca cgattccgta
    28201 atcggccacc ttgaagatcg gggcttcggg atccttgttg accgcgacga ccaccttgga
    28261 cgaggacatg ccggccaagt gctggatcgc tccgctgatc ccgaaggcaa aataaagatt
    28321 ggggctgacg gttttgcccg tttggccgac ttggtgggaa tggccgatcc agccggcgtc
    28381 gacggccgtc cgggacgccc cgacggccgc ccgcggaagg accgccgcca gatcggaaag
    28441 ccgggcgaat ccttccgggc ctttcatcgc ccggccgccg gcgacgacga tatcggcctc
    28501 ggccacatcg atcgccgccc cggactcgct caagatctcc gcgacttttc cggcgacggc
    28561 cccctccggg atcgcgaccg cgtgggggac aacctcggcc tcggggccgg gttccgcgtc
    28621 cagcggaaaa acgttgggcc ggagggtggc gatcgccggc ccggccggaa gggcgagggt
    28681 caggagggct ttgccggcaa aaatcggccg ggtgaagacc aggcggccgc cggaaacgga
    28741 aacggccgtg caatcggaag ccaggggaac gcccaggcgg gcggcaagcc ggggagcaag
    28801 atcccggccc atcgccgtgg ccggaaagaa gacggcccgg gccgctgttt cccggacgag
    28861 cccctcgacg gccagggcga atccctggct cgaataatcg gttaaggccg gattttggag
    28921 atgatggatc ttcgccgctc cggccgcgcc gatttcggcg gccgcccctt cgggagcgct
    28981 cccgacgagg accgcttccg ccggaaaacc cagggcttcg gccgccctcc gccccgcgac
    29041 gagcgtctcc agcgagcttt tcttgatttt tccgtcccgg atttcaataa agcaaagaat
    29101 catcatcctt ctcctcacag ggccttggct tcgttccgca gaagccggac cagttcccgg
    29161 gccgcttcct cggccgtacc gggcaggatt tttccggctt gtttggcggc cgggagctca
    29221 aggctcagga tctccacgcg ggggaaggcc tcctccccgg acaagccgag ctcggcgagc
    29281 gtcacgacgg gaacggtttt tttcttggcg gccatgatcc ccttgagcgt ttcatagcgg
    29341 ggctcgttca gccccttctg ggccgagacg acggccggga gacggaaggc gactttttcg
    29401 agggcgccct cgatttccct gcaggctgcg gcttcttggc cgctgatttc gagcttggtg
    29461 acaacggtgg cttggggaag cccgagcatc tcggcgacca tcggcggaac cgcggcgttg
    29521 tcggatccga ccgacatccg gccgaagaga atcaggccgg cgtcgggctg acgggcgcgg
    29581 acgaaggcgg ccaggacgcg ggcgatccgc aggggatcgt acgcctcccg gaggtcgtcc
    29641 ttgatcagca cggcctcgtc ggcccccatg gccagggctt tgcggagagt cgacaaggct
    29701 tcgtcggggc cgaaagaaac ggccgtgact ttgcccccct tggcttcccg aagccggatg
    29761 gcttcctcaa gggcgtactc atcataaggg ctcaggacga aattcaagcc ttcctcgacg
    29821 atggatttcg cctcccggtc gattcggatc cgggattcgg tatccggcac acgcttgatg
    29881 aagacaagga tattcatcgg tttctcctca cattcattcc ccgggcttgc cggctcgcag
    29941 cgagccccgg gttttcgccg gcgtctttct cccccctcag ccgtcgcacc ggcgaaggag
    30001 aagcgtggcg ttcgttcccc cgaatccgaa ggaattcgaa agagcgtaaa cgatttcagc
    30061 agaccgggcc cggcccggaa catagtccag gtcgcagtcc ggatcgggga cttcgtaatt
    30121 cgccgtgggc ggcatcgtct ggttcctgat cgctaaagcc gtgatggccg tctcgatccc
    30181 gccggccgcc ccgagggcat ggccggtcat ggacttggtc gagctgacgg ccaggcggac
    30241 ggcctgcgat ccgaagacct tcttgatcgc cgccgtctcg atccggtcgt tgagcggcgt
    30301 ggacgtgccg tgggcattga tgtattggac atcggccggg ggaacgccgg cgtcatccag
    30361 ggcggcttgc atcgcccgca cggccccgtc tccgtcctcg gccggggcgg ccggatgata
    30421 ggcatcgccc gtcattccat aaccgacgac ctcggcgtaa atccgggctc cgcggcggac
    30481 ggccgcgccg agttcctcca ggagacagat gcccgcgccc tcgccgataa cgaatccgtc
    30541 ccgcgttttg tcaaaaggcc gcgacgcccg gccgggatcg tcgttccgct ccgaaagggc
    30601 cttcatcgcg cagaatccgg ccacgctcag cggcgtgatc ggggcctcgg cccctccggc
    30661 gatcatccgg tcggccaggc cgtgctggat gatccggaag gcgtcgccca cggcgtgggc
    30721 gcccgaggcg caggccgtga cggaggccga gttcggcccc cgcgacttga agcggatgga
    30781 gatccggccg ctcgcctcgt tgatgatcgt ggcgatgaga aagaagggcg atatccggtc
    30841 gggtcctttt tccaggagga tcttgtgata ctcctcgatc gtcccgatgc cgccgatgcc
    30901 ggaaccgaca taaacgccgc accgttcgcc ggctaaaagg tccggcggaa tcccggcgtc
    30961 ctcggcggcc agaacggccg ccccgatcgc gtattggatg aacggatcca tcttccggat
    31021 ttctttccgg tcgacaaacc gcaggggatc gaatcccttg acctcggccg cgatccgcgt
    31081 ggcgtgccgg gaagcatcga aacgggtgat cgggccgatg ccgctcgatc cggagatcac
    31141 gttctcccag acgtcggaga cacctgtgcc caggggcgtg accagcccca ctcccgtgat
    31201 gacgacgcgt ctcagatcgt ggcgatggtc tcgggtttcc cggctatttg gcatggccga
    31261 gaatgtattc catggccttg ccgaccgtcg tcagcttttc ggcttcttcg tcgggaatat
    31321 ccaggccgaa ttcatcttcc agggccatca cgagctccac cgtgtccagg gaatccgcgc
    31381 ccaaatcctc gatgaaggag gcgttctccg tgacctggtc ctcgctgatg ctcagcttgt
    31441 cggccacgat cgtcttcatc tttttcagca attcgtctct gtccataatg gacctccttg
    31501 tgaaaatttg gatcgtcaca tataaaggcc gccgttgacg tggatcacct ggccggtgat
    31561 ataggccgcg tcgtcggaac acaggaaccg gaccgccttg gccacgtccg cgggttcgcc
    31621 gaaccgttga agagggatga gagcgagaaa ggactgcttg accgcctccg gaagacccaa
    31681 ggtcatgggg gtcgaaatat agccgggggc gaccgcgttg accgtgacgc cgcgtgaacc
    31741 gacttcccgg gccagcgact tggtgaatcc cacgacgccg gccttggtcg cggcgtagtt
    31801 ggtctgcccg ggattgccca tcgcgccggc gaccgaggac aagctgacga tccggcccga
    31861 gcgggccgaa agcatgctcc gaagcacggc cttggtgaaa ttaaaaatgc ccttgaggtt
    31921 gacggccacg accgcgtccc attcctcctc cctcatccgg agaagaaggt tgtcgcgggt
    31981 gatgccggcg ttattgatca gatggtcgat ttttccgaac tcctgaacga ccgaggcggc
    32041 ggccgcctcg gcttgggcga acgcggcgac gttggcttcc cggacggacg cccggccgcc
    32101 ggcggccttg atggaagcgg cgacggcctc cagcttttcc ttttggatgt cgacgaggac
    32161 gacgacggcg ccctcggcgg cgaagtcccg ggcgatcgtc tcgccgattc cctgcgaagc
    32221 tcccgtgacg accgtgacct tgtctttgaa aatcatcagg ctttaacctc ccctggggcg
    32281 accgtccggg cgagttcctc ccggattctg tcctgaaccc gcttgacgac gaattcccgg
    32341 gccagccgga cggcgttttt aacggcgttg gaattgctcc ggccgtggcc gacgatgcac
    32401 atcccattca ggcccagaag ctgggctcct ccgtattcgc tgtaatccac tcttttataa
    32461 atccgcttga gatggccttt catgagcagc aggccgatct tggcgaacaa attcttcgtg
    32521 atttcgcgcc gggccatgtg gaaaagcgtc tcaatgacgc cctcgctgac cttcaaggcg
    32581 acgttgccgg tgaatccgtc gctgacgatc acgtcggctt ttcccgcgta caggtccttg
    32641 ccctcgatat tgccgatgaa gttcaggccg gagaccgaaa gacggtcgaa ggtctgccgg
    32701 gtcagctcgt tgcccttgcc ctcttcttcc ccgatgctca tcagggcgac cgagggattc
    32761 gggcggccca tcacctgctc catgaaaacc ttgcccatga ccgcaaactg ggcgaggtcc
    32821 tcggtctggc aattggcgtt ggccccgacg tcgaggagaa gggtgtgtcc tttcagggtg
    32881 gggattaaaa gggagagcgc cggtttgttg actcccggca gggggccgag aatttggcgg
    32941 gccatggcga cgacggcggc cgtgttgccc atcgaaacga aggcgtcggc ctgtccgtcc
    33001 cggacaagct ggattccgat ccggatggag gagcgctttt tccgccggag ggcgaacatg
    33061 ccgtctccca tgccgacgga ctcgggggcg tcgatgatgg ggaaatccga aggttttccc
    33121 ccccagcggg cgacttccgg gctgatctgt tccttcgctc cgacgaaaac gacgtccagg
    33181 tcattttcct tggcggcctg gaccgccccc gggatcgtga tccggagacc taaatccccc
    33241 cccatcgcgt caacggcgat tttcatccga cgagaatcca gaattatttt tcggcggctt
    33301 tgatcaccgg tcgaccgcgg taataaccgc attccggaca agcatgatgc ggcatcttcg
    33361 gcgttccgca attgggacaa gaggagaacg acggcacggt catgaaatca tgcgcccggc
    33421 ggcggccctt gcgggcatgc gagtgtctgc gtttgggatt cggcatttat tttcccttat
    33481 ctttcctaag aatttttagc ggctcgagac ggacatccgt ttccttgacc tggcagccgc
    33541 aggctccctc gcgccggatt ttgccgcaca cggcgcacaa gccctcgcag ccgggagagc
    33601 acaacggccg ggccggaaac gtcaggttca attgttcgag gatcacctcc cggaggtcga
    33661 tccgccggct ccggtagaac agccggtcgg catcctcgtc gtcgaattct tccttcagct
    33721 cctgcatctc ctcggggaga aaaaccaagt cgaagcggga atcgacggga aactcaaaag
    33781 gcgcgaggca ccgtccgcag gtgaagcgca ggacggtctt cacgcggccc ttgataaaga
    33841 cctcctcttc catccgcttg accgagagct cggcttgaac gggttggagg aagacagcgt
    33901 cttcctcgac caggtccacg ctcggaaaat cgaaattccg gctgatgtcc agtccgtctt
    33961 tcggtaactt atctatatca ataatcatag gttacacccg caaaaggtcc gcttattata
    34021 ccatgtgact tccctttgtc aaccaaccgc cggccggcgg cccgagggcc ggagcgtccc
    34081 cggcgccggc ggcggccggg ccgccccggc cgccgccggg ttccggcgta ttttcttctt
    34141 gattaatctc tccaatcgga ttatttataa gacatccgat gaacggccta tcgacgacca
    34201 ttctttattc cgggatttcc ttcctggtcc aaacccagga gggcggaccg caaacccatt
    34261 ccctggaaac gctgatttac cggcgcggcc agctcgtcta ttcgcgcaag tcctcctgca
    34321 cggcgctcct tgacgatccc gagcgggccg aacgcttgac gcatctcatc cgtgatcagc
    34381 atcaggccgt tctcgatgac atcgccgggg gacggctcga cattcctctg ctcaaatcct
    34441 gacgatgccc ggaaaaatcc tctatctcat cgacgggaat tccctgctct accgatctta
    34501 ttacgccgtt cagcggctct cgacggccgc cggattcccg acgaacgcga tctacggttt
    34561 tctgatcacc ctgcgcaaga ttttggacga ggccgccccc ggtcatctcg gcgtcgtttt
    34621 cgacgctccc ggggaaaaaa tccgccggga agtttaccag gattacaaag cccaccgaaa
    34681 gcccatgccc gacgacctgg ccgcccagct tcctaagctg aaagaagttc ttcaagcctg
    34741 ccggatcccc ctgttggaga gcccccgcca cgaggccgac gacgttctcg gctgcctggc
    34801 gctccaggcg gccgcggcgg gatttgaaac cgtgatcgtc acgacggaca aagacctttt
    34861 ccagttggtt gactccaaga tcaaggtttt caacccggcc aaggagaaat ttttcgacga
    34921 aaagaccgtg ggcaaggagt tcggcgttcc ccccaaccgc gtggtcgacg tcctttccct
    34981 ctggggggat ccgtccgaca acattcccgg agttcccgga atcggcgaaa aaacagccaa
    35041 ggccctgatc accgagcacg gaagcctgga cgcgatcctc gaacatcccg agcggatcaa
    35101 gaatttacgg ctccgggaag cggtgaaaaa caaccgggac aagatcgaaa tcagccggaa
    35161 gctggccacg atcgaatgcg ccttggacgt ccccttcgat ccggtcgcct ttcaggtcga
    35221 ggagccggac aagccggccc tggcccggct gtattccgag ctggaattta cgagcctggc
    35281 cgccgggatc tccgccggcg ccccgctcga gaagaaaaaa acccgcgttt accggacgat
    35341 tctcgccgtt ttggacctca ggcgcctgac cggccggatc cgcgaggcgg gtttcgtcgc
    35401 cctcgacacc gaaaccgacc atatctcccc gacccgggcc cggcttgtcg ggatgtcctt
    35461 ctcccttgag ccgagcgagg ccttttatct tcccctgcgg catgattacc tcggagcccc
    35521 ggctcagatt cccctcggcc aagccctcga catcctccga ccggtcctgg aggatccgaa
    35581 gatccgaaaa atcggccata acatcaagta cgacctgatc gttctccgcc gggaaggcct
    35641 cgacctccag ggcttggacg acgacaccat gatcctgtca tatcttctgg aaccgaattg
    35701 gggccgccac aacctcgacc gcctggccgc ggcttatctc caggagacgg ccattcccta
    35761 ccaagccgtc gtcggcaagg gcaagaacga gatcacgatg aaccgcgcgc cggtcgaatc
    35821 ggccgccccg tatgcctgcc aggacgccga cttcgccctg tccatcgccc ggatcctccg
    35881 gccgcgcttg gaacaggagg gactcgacag cgtctaccgc gacctcgagc gtccgttgat
    35941 ccggatcctg gcggacatgg aaacggccgg cgtccgggtc gacgaggcgg ccctccgcag
    36001 cgtttcggcc gagctcgaag ccagcctccg cctccttgaa aaacagatct acgagcacgc
    36061 cgggctcgag ttcaacatca attcccccgc ccagctgtcg tccgttctct ttcagaagct
    36121 tcttctcccg gccccgaaaa aagcccgcgg atcgggcgcc gtctcgaccg ccatcgacgt
    36181 cctcgacggt ctccgcgatc tccaccccgt cgtccccctc gtcatcgaat accgccagac
    36241 ggccaagctc aagtccacct acgccgacgc cctgcccgcc ttgatcgatc cggccgacgg
    36301 ccgcattcac acctcctaca accaggccgt gacctcgacc ggccgtctct ccagctccga
    36361 cccgaatctg caaaacatcc cggcccgtgg accgatcggc aagcgcttcc gcgcggcttt
    36421 tattcctgaa aagggccgcc ttcttctggc cgcggattat tcccagatcg agctccgggt
    36481 gctggcccat ctctccgagg atcccgccct cattgacatt ttccgcaggg accgggacat
    36541 tcatgccgag acggccgacc gggtcttcgg ccgaaacgcc ggcgggacgg atgaggaacg
    36601 gcgacgccgg gcgaaaatca tcaatttcag cctgatctac ggcgcctcgg ccttttccct
    36661 ggcccgggag cttgaaacgt cgacccggga agcccaagaa tacatcgaca agtactacga
    36721 acggtttccc ggggtcaaag cctatctcga aggactcgtc gcctcggccg cctcccgggg
    36781 atacgccgaa acgctgttcg gccggcggcg tccggttccc gagctcaagc agtcggaccg
    36841 gacggcccgg gatgccgggc ggcggatcgc ccttaacatg cccattcagg gaacggcggc
    36901 cgatatcatg aagaaagcca tgatcgcggc gggcgcggaa ctcgggcggc gagcgctcgc
    36961 cgcccggatg attctccagg ttcacgacga gctcgtcttc gaagcgccgg aaaaagaacg
    37021 ttccatcctg gacggcctgg tccgaaacgt catggagtcg gcctgccccg gcttccgcgt
    37081 gcccttgaag gtttcgctcg gctggggggc cagctgggcc gaggcgaaat aatcctccgt
    37141 ccgcgagaac gcctcgttcc aggaagggac atgtccgcat gagccgcgaa caaacagagg
    37201 cgcccgggaa agccggcccg gccggccgtt ttctattcat ctcggaaatc ctgaaccggc
    37261 cggtcgtctc ggccgccgga gagacggtcg ggaggctgca tgatcccaag gtccgcctga
    37321 acgagccgta tccgccgatc gtcggcttgg ccgtccggaa acgccgtctt tcacctcatc
    37381 ctcgttttca tgctcatcct gaccaatgac aaaaagctca tgggtgaata tgtcaacaaa
    37441 aaaggataca atatcgtggc ttggagcacg atcgtgatcc tcgtcgcctt gacgacggtc
    37501 tacctcgggc tccttctcgg aatccgatga catgaccgaa gccgccggcc gccccctcat
    37561 cctcatcgtc gaggatgaaa tcatcatcgc catggaaatc caggtccgat tggaaagcgc
    37621 cggcttcgcc gtatgcggtc tcgcttcgtc cggggagaag gccatcgccc tcgcccggga
    37681 aaaaaatccc gacctcgtcc tgatggacat caccttgaaa ggcccgctcg acggcctgga
    37741 aacggcgggg cggatccaag ccgaaaagga cattccggtg atttttctct cggccacgga
    37801 cgacgaggcc gtgctgcgcc ggatccggg
//
LOCUS       DAOOXH010000014        37378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_932_length_37378_cov_8.948021, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000014 DAOOXH010000000
VERSION     DAOOXH010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 37378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37378
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_932_length_37378_cov_8.948021"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="PKV64_03170"
     CDS             complement(<1..1035)
                     /locus_tag="PKV64_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPN16116.1"
                     /translation="MNTILIVDDERSILELLSVILKKEGYAVLVNPGTPSIYEDIEQK
                     EFDLVISDIKMPQVDGMEVLRRVKAEKPTVPVIMITAYASVKQAVEALRLGAMDYIMK
                     PFDIEEIKVLVANGLEHRRLLEENKILKQALREETSFENMIGKSRPMQEVFTLIEKVA
                     ATDSTVLITGESGTGKELAARAIHANSRRRDRAFVSINCAALPENLLESELFGHVKGS
                     FTGAISDKKGMFETAHAGTIFLDEVGETSPWTQVKLFRVLQERMIRRVGGNEEIPVNV
                     RIIAATNQNLRKRIEEGKFREELFYRLNVISVDMPPLRRRPDDIPLLIQHFLRKHCEK
                     MGRRIKRLTPE"
     gene            complement(1032..2324)
                     /locus_tag="PKV64_03175"
     CDS             complement(1032..2324)
                     /locus_tag="PKV64_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN16117.1"
                     /translation="MRTLKSNVRWLLIWRLVVITSLFVAAVVIQLATASFLPLSPFYF
                     LIVTEYATGLVFFLLLLFNTRYRAQAYAQILIDLAVITGLVYISGGVGGQLYFLYVFA
                     IIAAGLVLGGRAALMTAAFSAIVFGAVADSLAYGWILYFRPDQGAEIEIGQVLYTVFM
                     AWGLFGVIAVLIAKLSSSLQKTRAELDAAQQEINIRERDAAAGRASAFIAHEIRNPLA
                     AISGAVQVLQNDLDLGGEQARLMDIVVKESHRVSQSIEQFLCLASPGKQTYSVFRLAD
                     PLVETLTMLRMSGEMSDRIAVGGNYEASPFLYFGSPGQFKQVFWNLLGNALKAMPDGG
                     GLSIDFFRPDKNVLAIRIVDTGRGMTAAEKARMFEPFYTRFEGGRGLGMSVVRQIIES
                     YAGKIQVQSEPNVGTDIKIILPYREFGAKGNSGAADCR"
     gene            complement(2332..3537)
                     /locus_tag="PKV64_03180"
     CDS             complement(2332..3537)
                     /locus_tag="PKV64_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPN16118.1"
                     /translation="MPVYIWKGTNKFGDGVEGERVAANAEELARLLQREQIKVEKISP
                     VRGKFNLPFLKGEKVKQKDLAIFARQMAVLIDAELPLIQGLNIMAEQTRNAYFKRVIT
                     QVRAEVEAGSSLDQALRKFPKVFDDLFCNLIASGEQSGTLDTMLQRLADYIENSVKLK
                     AKVKQAMTYPVAIFAFAIIVAVFMLWKVIPTFAQIFVDLGAQLPGLTAFIMNLSEFVQ
                     KYILFLVLGTVGALFLFRYYRKTQGGRWTTDGLLLRLPLFGSLLTKVAISRITRTLST
                     LLGGGVSMLESIKITATTAGNVILEKSVMDIRKQVTEGRSLTDSIKSTGRFPFMMTQM
                     VSVGEATGTLDKMLTKLADFYDSEVDNTVGTLLSVLEPFMMVFIGGLVGTLIISMYLP
                     IFSLMGQIQ"
     gene            complement(3553..4650)
                     /locus_tag="PKV64_03185"
     CDS             complement(3553..4650)
                     /locus_tag="PKV64_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPN16119.1"
                     /translation="MPLSMHELLKMAVDADASDLHLTTSVPPRLRVHGELKSISHPPL
                     TATETKQLVYSILTDKQKKLFEEKMELDFSFGVKDLGRFRANVFMQKGSVAAAIRRFL
                     PSMWSFQRLGIPSRIAQLCFLPRGLILITGPTGSGKSTTLACMIDHINTERAVHIITI
                     EDPIEYFFTPKKALINQRELLSDTLSYSNALRSVLREDPDVVLVGEMRDQETVEATLR
                     VAETGHLTFSTLHTNSAPETISRIIDIFPPQYQAQARVMLSMSLEGVMCQSLLPRADG
                     TGRVLAMEILIPNPAVRNLIRENKIHQIYSQMQLGQEKFGMLTFNQNLCDLHCRKVIT
                     LETAMSATSNPEELTDLIQRRAAGVGVAVKR"
     gene            complement(4661..6394)
                     /gene="pilB"
                     /locus_tag="PKV64_03190"
     CDS             complement(4661..6394)
                     /gene="pilB"
                     /locus_tag="PKV64_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HPN16120.1"
                     /translation="MASTIGDVLLKEKILTAEQLKTAQDFQKKHDILLSSAIVSLNYV
                     SGEEMAQGLSRQLGYPYIDLDQFEVYPDVISLITADTAKKSLIMPIHRIRSFLTLAMV
                     DPTDMDVIEDIRFRTGLSIQPVIASESSILNAINKYYGAGTALRVKKNIEEIELADDS
                     KISAIDQLEEIKDEPDEEEIEEVPDEAPIITLVNNIFVEAIKKGASDVHFEPYEKSFR
                     VRYRIDGILYETMNLPLKFKNPLTSRVKIMSNMDIAEKRLPQDGRIKMRAKLDKAVKK
                     EVDMRISCLPTIFGEKIVARILDRDNLNIDLNKLGFESESLAVFQRAIARPWGIILVS
                     GPTGSGKTNTLYSAISTLNGMEKNIMTAEDPVEFYIPGINQVNIRDEINLNFAAALRA
                     FLRQDPDIMLIGEMRDQETVDVAIKAALTGHLVFSTIHTNDSAATIHRLMNMNIEPFL
                     IADSLALVVAQRLVRRVCKKCAAPTKLPVKSLVEMGFTSEEAEKAAVMQGRGCDICHN
                     SGYKGRTALFEVMELNPTLREMILLRAQSKEIKQKAVEAGMITMRRSGLIKIMAGITT
                     PEEVLRETVRD"
     gene            complement(6603..8300)
                     /locus_tag="PKV64_03195"
     CDS             complement(6603..8300)
                     /locus_tag="PKV64_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HPN16121.1"
                     /translation="MNQGLSLPGWISFICGWGVILGLSVYCMSRVLFTKKKAVVETGP
                     GGRPDRWATRIGLVLAMAGNAIGLGNFLRFPAKAASNGGGAFLIPYFCALILMGVPMM
                     WVEWTIGRHGGAHGHGSGPGMFQVMWKHPFSKYIGAFGIFIPFAVAVYYLFIMAWCLG
                     YAWFSATGRYFGLVNQEAMSSFLKGFQGVESNRYFSSLIPALLFLAVCLAMNYFFLSR
                     GISKGIEVLARHGMPLLFLFGVILVIRVLTLGTPDPAHPDWNIANGMGFMWNPDFSRL
                     TQGSVWLVAAGQIFFTLSLGQGMILTYASYVRENQDITLNGLATSTVNEFAEVVLGGT
                     IAIPVAVAFFGLAQTEVIARGGAFDLGFVSLPLIFQKIPFGQLFGAMWFLLLFIAGIT
                     TSVAFLQPLVAFLTEEFHISRKKAVNRSFLFLIGAVLFVVFFFRFGVLDEFDFWISTF
                     GIVVFGALEIIVFSWIFGLKNGWEEMHKGADFKIPGVFKFILKYITPAYMLVILGVWT
                     WQDAVGKFLMKGEPAERRPYLWAARLLMALIILSICLLVRKAWRKKSPVAPSFPRGD"
     gene            8807..9985
                     /locus_tag="PKV64_03200"
     CDS             8807..9985
                     /locus_tag="PKV64_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPN16122.1"
                     /translation="MEKTSPYHNLVRFLKEKGLYPHEKAYDQPDGAEVVFRGKSILSL
                     ASVDYFNFAQNPEIKKTACEAIRKYGAGGFSSRLSMGNLSIHMELEGALAEFMGKESV
                     LIFNSGYLANLALITSLLPRNATIFLDQKCHLSIHHACLMSQKKYFRFSNNDMSDLER
                     LLQKHRDEPEKWIVTLGVFSSNGLLAKLDEIAVLARKYKARIFIDDAHGIGVYGDRLR
                     GAADHFGVLDEMDLIMCPFQMAFGNIGAFVVGKRYLLQAAHTDAWPYMFTYNIPPVSA
                     ASLLKALEILGREGREYQKKLWENVAYLRRALTEIGYKILNPNGHIIPILIGDDVKVC
                     AFAQRLFHQGVWVQPFYYPAVPKGQATVRVTCTAGHTRSELDHAIRAFADASLWLANS
                     "
     gene            9991..11940
                     /locus_tag="PKV64_03205"
     CDS             9991..11940
                     /locus_tag="PKV64_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPN16123.1"
                     /translation="MPLTAFLSALLCLLTGVFVYVKGKNNPVNRYFLVFNILIFFWNS
                     GDFVIPLMGPRISAGLIYDRLSQIGGILIAPWFILFYLTLTGLSFREIVRTKIFRVFV
                     AVAVLLLFLAPTPLLIERIQVEPFKEFPGKLLYVEITAVLASIFFGLRHLFIARKTVN
                     LAQRVRLTYFGVAVLMGILSAVAWFVIITFVPGLSTKFIYVFEVTYVLLTAYAILRHH
                     LMNIDVIVKRTALYTILSTFVIGVYIAIILIFEVVFRSLTGYSSLAEKIIAAFIIALT
                     FQPVRTLIEKTIDRIFARGKVDYQDALAKFTRSLGSVLDLRELVDLLVSISGILRAKN
                     LAVMLRDEQRGRFKIVASTGLDSPLHKIGFDDFNDIVRVLNTTNKIITTDDIKRLRSE
                     NVYKNLPGEVSALRAEVILPIFVKGELDGILFLGEKRSEERYDRNDFSVLATLADEAG
                     IAMENAKLYGSVKRTYFETVQALSLAIEANDEYTRGHSDRVTKIAVKIAKRIRLTRER
                     IDTLKFAGILHDIGKIGVIKEVLHKPTKLSDLEFALIKNHPVVGEEIIKPVAFLDKIR
                     PVIHHHHERFDGSGYPDGLRGKDIPFLSRIITVADAYDAMTSHRPYRSALSHETAVAE
                     ITKYAGTQFDPEIVQAFLEIAGTLI"
     gene            12092..13243
                     /locus_tag="PKV64_03210"
     CDS             12092..13243
                     /locus_tag="PKV64_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16124.1"
                     /translation="MNIGAFGRVIAGVLLLAAAAGAYLRPKIGCDASSIHCHTNPSAG
                     PQATIIRIFNNGGATLSWRISGGAPWLKISPLSGVNDGTVTVTVDPAGLEPGRYESSF
                     MIKAKFAVNSPLKIKVILDVATDRLLAGFRASRYGVDPFPDPAYWASVGAGMASRFPG
                     AGMGCVWIVGVFLDNGDCGLNFPSPGGDYPSIHFLDEDQNEAALSFFDALDLSVWLQV
                     EPGRASVPKLIDLVLRRYGHHPCVKGVGVDVEWYKTDSAHPEGRRVNDLSALLWSLRV
                     RSYNPAYTLFLKHWLIDKMPPRFRLGLWFINDSQQFGSLGQMASEFKAWGEHFWPSSV
                     GFQFGYEADKPWWSLLADPPRNIGDAIRAVVPNAFGLFWVDFTIQDIFP"
     gene            13260..14474
                     /locus_tag="PKV64_03215"
     CDS             13260..14474
                     /locus_tag="PKV64_03215"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPN16125.1"
                     /translation="MKAFFKKKRVLVTGGTGHLGSALIHHLVQNLAMDPAAIRVFYLQ
                     GSTTRSLRDIPGLDYAPGNILNPDDVERACAGIDYVFHAAASTSFDPRSKRLQWMINV
                     EGTRNLLESVRRSPTIKKTSFTSTVNVLGVPNPAGSAGNFENSDPYTNRPRLHAFRSR
                     EETLSFIDMVRERRSSPWEKRIGIGYFDSKLAAQELVNRYVRDHGLNVVSVLPGTCFG
                     PYDVLGGNGIYLLRIYHGALPGILKGGFSATHVMDVVEGHLLCLVSAAAGSAYIINGP
                     RKDDLHFTDVARTIAEVLGRRYPEKKIKRPSMIVPSGPAWAAARASELVAAVRRRPCL
                     LSRAMVKAGSQPLFYTHENAERDLGYRPKRTFRQAVEEMAAYFEKEGLFSALTPRPFP
                     AGPPETGEFPGF"
     gene            complement(14670..14743)
                     /locus_tag="PKV64_03220"
     tRNA            complement(14670..14743)
                     /locus_tag="PKV64_03220"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14707..14709),aa:Arg,seq:tct)
     gene            complement(14779..14853)
                     /locus_tag="PKV64_03225"
     tRNA            complement(14779..14853)
                     /locus_tag="PKV64_03225"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14816..14818),aa:Pro,seq:tgg)
     gene            complement(15175..16956)
                     /gene="mutL"
                     /locus_tag="PKV64_03230"
     CDS             complement(15175..16956)
                     /gene="mutL"
                     /locus_tag="PKV64_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPN16126.1"
                     /translation="MTRIAVLPDALARKIAAGEVIERPVSVVKELVENALDAGASAVA
                     VDLAAGGKSLLRVRDDGCGMSREDAALALVRHATSKISKEDDLFAIATLGFRGEALAS
                     IAAVSRLTLRTSEGGDEPGTLVESEPGGAFQVRDSAFPRGTEVEVRDLFANLPARKKF
                     LRSDSSELGLIVKMLTNIALARPGLRLIVRHGARTVLDCPPAAGLRERLFQLFGKDAL
                     DKMMEADLADGPVRVSGYVSRPPYGRPNRGRQAFFVNRRPVRDKILSAALQQAFSGLL
                     EKGLFPEAFLFVDIPPGEVDVNVHPAKTEVRFRDAQLFFPLLRRVVERARTRVLGVRD
                     LSEILADGAGVPPGGRPAPAGTENGEGAGPEREEPECSGVGEGGRTYIRGTDFADFPA
                     AGVLPRAAESPQVLGQLGSAYILAEEAGDLLIIDQHNAHERVIYDRYVEIDRERKWPV
                     KMSLLPLVFELSPVQEIGLEEAGPMLRSSGFRVEPMGGRSYALREYPDVFRPEEALSV
                     VLAAADESKKGAPAPAKDRLLAAMACKSAVKAGEYLPREKMEFLVRELFRSANPGVCP
                     HGRPIVVRIPRSQIERGLRRPVSDPAS"
     gene            complement(16953..18224)
                     /gene="mtaB"
                     /locus_tag="PKV64_03235"
     CDS             complement(16953..18224)
                     /gene="mtaB"
                     /locus_tag="PKV64_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HPN16127.1"
                     /translation="MTSFFIHPFGCRVNQAEAFDWAAALQKKGWRLAEEDSQGEIVII
                     NTCTLTARADRDGRRYIRKIAQENPRARLVVTGCLAERDPEGVKALPGVWRVVANADK
                     DALAGQCAAGESPETAVRAEEPVSYRVRALVKAQDGCDMACAFCVIPSVRGRSRSVPV
                     LDLWTRVEEFVSAGYAEIVLAGIHLCSYGRDLRPPSSLLELLSGIEELPGDFRLRLSS
                     LDPRLLSRPLLDFLTGSRRIAPHFHLSLQHGADRILRLMGRESTNASYRELCAELERR
                     SPEANLGADIIVGFPGETERDYEETVRLLEESPLAYAHVFSYSARPGTKAAERPAVDR
                     ATVKSRADRLRRLSGRKNLAYRRRLIGRTLPGVVIQSKPGGLEVLTSNYVDVLVSGAA
                     APLGSPVHVRIAAAGERDTTGELRGRDGFRS"
     gene            complement(18221..18715)
                     /gene="purE"
                     /locus_tag="PKV64_03240"
     CDS             complement(18221..18715)
                     /gene="purE"
                     /locus_tag="PKV64_03240"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPN16128.1"
                     /translation="MMDVVILIGSASDLDVAGEALEILKQFGVTHVLEVTSAHRSPER
                     TIELIRRFEDGGAEVFIAVAGKAAHLAGIVAAHTIRPVIGVPVESGGLGGLDALLSTV
                     QMPKGIPVATMGTGKSGAANAALLAVQILALKSPDLRTRLAAHKAGMAVQVEADSKKA
                     RQSL"
     gene            complement(18742..20016)
                     /gene="purD"
                     /locus_tag="PKV64_03245"
     CDS             complement(18742..20016)
                     /gene="purD"
                     /locus_tag="PKV64_03245"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPN16129.1"
                     /translation="MKALVVGSGGREHALAWKIAKSPKISKVFCAPGNGGTRLVAENV
                     PISETDIPALADFAVKEEIDLTVVGPEIPLSLGIVDEFEKRGLRIFGPSKKAVELEAS
                     KVFAKQFMDRHRIPTGKFRIADSPESARAVLAGGEFGFPVVLKADGLAAGKGVLVCRN
                     EEEAEAGVKEIMVEKRFGGAGGRILIEEFLRGREVSFIVIADGAKAIPLVTTMDHKTV
                     FDGGKGPNTGGMGSISPSPFLDRKMYARIMSTIILPTVTRMLEEGRKYKGALYAGLML
                     TADGPKVLEYNCRFGDPETQPQMLRLESDFVDLLMASIAEDILADEVTWNRRAAGCVV
                     LASGGYPGSYEKGKTIQGLEDADRLGAVVFHAGTKFEKGRYRTEGGRVLGVCAAEPRL
                     SETMNRIYRAVEKISFEGMHYRRDIGAFESAP"
     gene            complement(20044..21483)
                     /locus_tag="PKV64_03250"
     CDS             complement(20044..21483)
                     /locus_tag="PKV64_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16130.1"
                     /translation="MTMPLSEKTAKLVDRIERNSLWRQRECLNLIPSESTPSLLVKIC
                     EMADPSGRYAEHRTMKGEEVYFYQGIDFIKDVEIEARRELAAFFGCVDVELRPVSGQM
                     ANEIVFKGVTKFLNRGRAEGQVSRRMRLVMNNDLGKGGHLSSQPMGALYNWIEEDPAT
                     GKENVVNFPVRKDNPYKIDLEALAALLEVKKPELIVFGKSMFLHPEPVAFTAGIVKDW
                     PKRPVIMYDMAHVLGLYGAFQEPFQDGADIVTGSTHKTFFGPQRGLVAGNIAPSSPLK
                     NLWTDIRGRAFPGSTSNHHLATLLGLLMASYEMNAFKKELQTAVRANAKSFAASLKAQ
                     GIPVEGDPADGYTDTHQVIIRVSSFGPGMEIARRLEDNNIVTNYQALPDDETFLVSSG
                     IRMGVQEMTRFGMTGKDFDVVAGLIADCVIRNRRVKDDVAAFRRKFSEMKYCLPAAEA
                     VPLAARVFAAAMPASNIGRAFAEAVGRVS"
     gene            complement(21521..21973)
                     /gene="rpiB"
                     /locus_tag="PKV64_03255"
     CDS             complement(21521..21973)
                     /gene="rpiB"
                     /locus_tag="PKV64_03255"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPN16131.1"
                     /translation="MNIALASDHAGFEMKEMIARFLTGRGVDFADLGSRAGESVDYVD
                     YAAAAAEAVLAGTCDRAILVCGTGIGMAMAANKFPGIRATVCWTPATAEMARRHNNSN
                     CLTLGGRILDNQTALEIVRVWLETPFDGGRHERRLAKIAEIEKKSCGC"
     gene            complement(21970..23532)
                     /locus_tag="PKV64_03260"
     CDS             complement(21970..23532)
                     /locus_tag="PKV64_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPN16132.1"
                     /translation="MKNKTLFLLLGAGILFGAAVRPGDPWVEGREKVGAMAGLIQAEY
                     FRTAEADKMARAAVRGMLETLDPHSYLLDPDNLARMSEEQRGAYFGLGIQIQKHMDKL
                     VVIAPIEGTPAWRMGLQAGDTITHIEGESTVLISANDAVSKLRGPKGTKVAITVAREG
                     LEKPFDLTITREEIPLYSVPYAFMLDEATGYVFIRYFAENTTAELEAKLETLRNAGMK
                     NLVLDLRGNVGGPLFQAIGVADEFLARGESIVSIRGRNPDFDRQYPATRNGQYEAVPL
                     VVLISQGSASASEIVAGAVMDHDRGLILGEDSWGKSLVQKMFPLSEDTAMALTIARYY
                     TPSGRSLQRDYSHLDDYFLDTKVLAESDREVKYTDKGRKVLGQGGITPDLTVVFNLLP
                     YTAELRIRGAFFAYARKFTSRQTPLSKKFVFPADGNAARPAPGMIQLSRVFLADAAVL
                     EDFRAFLIENKMPADETKFKEAAVEVKRELEREIASLLWGGEEGWRAFEKNDPVILQA
                     LEVMPEAARMIQ"
     gene            23649..23921
                     /gene="tatA"
                     /locus_tag="PKV64_03265"
     CDS             23649..23921
                     /gene="tatA"
                     /locus_tag="PKV64_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPN16133.1"
                     /translation="MFGNIGLPELIVIMGLALLIFGPRKLPEIGRTIGKAVREFKKST
                     EDIKEKFEEQIRVEEFKSLQADLKKDLREADLRKDLSGNGEDSKSS"
     gene            23896..24684
                     /gene="tatC"
                     /locus_tag="PKV64_03270"
     CDS             23896..24684
                     /gene="tatC"
                     /locus_tag="PKV64_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPN16134.1"
                     /translation="MGKTRKAPDEMTFLEHLEDLRTRLFRSVLSLFIAVFPAYIFSKD
                     IFHFLSRPLTRFLPEGEQLAFRTLTEPFMLYIKVSFLAALFAVSPYIFYQLWKFVAPG
                     LYQKEKKYVFPFVLFTSVFFIGGAAFAYYIAYPFACRFFLQLGSDFQAVITVNDFFNL
                     TIKMLVGIGLVFELPILILFLSKMGLITSRWMVRKFKYAVLVIFVIAAIITPTPDMIS
                     QSILAVPMLALYSLGIAVAFFFGKERKDRRERRAKKKAAAAEAS"
     gene            24681..25223
                     /locus_tag="PKV64_03275"
     CDS             24681..25223
                     /locus_tag="PKV64_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPN16135.1"
                     /translation="MSSLRPRLIGLTGPNGAGKGEAAAFFAARGYAHRSLSDIIREEL
                     AAAGLPADRDHLIAKGNELRRKGGPDILARRLLKLVTGPTVIDSIRNPAEVERLRKED
                     GFILLAVDAPAPLRFERARRRGRDESAATLEEFLRKEAEERSSNPAAQQIHRCFEMAD
                     AVVSNDGTLEELHSRLEAFL"
     gene            25220..25726
                     /locus_tag="PKV64_03280"
     CDS             25220..25726
                     /locus_tag="PKV64_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HPN16136.1"
                     /translation="MNTPRISKDEYYLGIAREVARRSTCWRRSIGALIVRDDQIVSTG
                     YAGAPRKTRDSIEHGFCLRDKLQIPHGQRYELCRSVHAEQNAIINAARAGVSLLRGDM
                     YIFGSVPDGRPVDAFPCFICKKMIINAGLVRIVCSTAAGGSTAFLVDDWARAWREGDI
                     LDDTHQYG"
     gene            25704..27869
                     /locus_tag="PKV64_03285"
     CDS             25704..27869
                     /locus_tag="PKV64_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16137.1"
                     /translation="MTRINTAERKSPWPRTAAFLLAAALLAPAAGPGEENLLKLLGIA
                     VSAESIDYVVQKTPFQLHTLLTEQRHPKTWMLGRRIAADTAEGLRMLFGVDEDIALKL
                     DGLVRDPEQLGRVERLTEAFAAAMLGRRKIYMYGCGATGRLAKQVESAFWRPFWGGLK
                     KNEPGLWAKCSARLDPAVEDELIGEMTGADRALISSLEGFEDLQLIGRLQLHDRGIKP
                     GDVVVCVTEGGETSSVIGTIRAALEDRKARPGYDPAESRKALFFVYNNPDDLLRPFER
                     SRIVLEDPGITKINLTTGPQAITGSTRMQATTIETFVVGSALETAVERVLRTMLSKKE
                     MGRLGFSRETSLEDRLKAFAPLLPEIKRKAGEIARLTDLEARAYAGGRMSTYFAREGL
                     ITVFIDSTERSPTFRLAPLDTVREPRRKSWIQVWTAAEARAQAWEAFLGRPFRGLDPL
                     FYRKPFENEIDDVYLRDKALQSLARAGDDQAALYDFSFGGTNVQKRAPQKGDVAILAL
                     VGDEIGESRNPRSTFFRFAELAAEKGAALGVLSICDLNKEKKAAGGVFPSPRIDVEFA
                     VSRENDPMGIRQQIALKMILNAHSTAVMAKLGKVVGNTMTNVSPSNLKLIGRATYLVQ
                     LHANDILGKPEWVKAFGARPPVSYAEANAVLFDSIAFLKDRPQGAAISPEVALSIVRI
                     LESLRRKIGITAEEALAIVRKTSLQDYLDQAPRPPAPGE"
     gene            complement(27893..29029)
                     /locus_tag="PKV64_03290"
     CDS             complement(27893..29029)
                     /locus_tag="PKV64_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPN16138.1"
                     /translation="MDDWSQIARGSLRTPDQVACAFDLDVAEVRRISRHFKTLITPYY
                     AGLIKTKGDPIYRQVVPDPAELVEHSGVSDPLAEDHDSPVPSVVHRYPDRLLFLVSHR
                     CASYCRFCTRKRKVGDPKKIHPKAIEEGLEYIRTHEEIRDVIVSGGDPLMLSDARLEY
                     ILTRLRAIPHVEILRIGTRVPCFLPQRVTPKLAAMLRKFHPLFVNVHFNHPDELTPEA
                     NQALGLLADAGIPLGCQTVLLKGVNDDPLVMRRLMQKLLMARVRPYYIYQADYVSGTD
                     HLRTTVQKGIEVMEALRGWTSGLAVPYYCIDAPGGGGKIPLLPKYIQSISDEQVVMRN
                     FEGETFVYPQPKSDRPAPEKKAPRKERRPSISPCYDLTGLPPIG"
     gene            complement(29043..29516)
                     /locus_tag="PKV64_03295"
     CDS             complement(29043..29516)
                     /locus_tag="PKV64_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN16139.1"
                     /translation="MIRPMTPADRPAVLALIEATGFFRPDEIAVAEELIDITLGRPEQ
                     EDYAIVVVTDETGAVAGYMTYGPTPLTRGTYDLYWMAVDPKAQGRGYGRILVEWLEDH
                     VRRAGGRLIVIETSSSEKYEPTRRFYLGLNYTETARIPDFYGPGDGRVIYTKRVS"
     gene            complement(29513..30535)
                     /locus_tag="PKV64_03300"
     CDS             complement(29513..30535)
                     /locus_tag="PKV64_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPN16140.1"
                     /translation="MSAKSGPNLLVGIAFNAYDPLTANKVDRPSEESVEQTAKEVLTA
                     VTELGYAAFIIPLQKSFMNFCQRLKTLNADVLINLCEAFLGRPQLEANVAAALELLGV
                     PFTGNDSRTLALCLDKYKTKAVLKSFGLPTPPAVLAESPDQKINLPFPLIVKPNNEDA
                     SLGIHPESVVRDEDGLRRQLGHIQDVFGEPALVEAYIEGREFNVAVRDAEKTEALPVS
                     EIEFEGMPENQPNICSYAAKWHPDDVLFQSTPPRCPASIDDGLRTLLQEIAVKAFSAC
                     ECRDYARVDYRVDKKGRPFILEVNPNPDISLNAGYARALGAAGVEYKNFWKQMIDKAL
                     LRGEKR"
     gene            complement(30532..31623)
                     /locus_tag="PKV64_03305"
     CDS             complement(30532..31623)
                     /locus_tag="PKV64_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPN16141.1"
                     /translation="MKVALLFSSKKGMAASLRQSVAEATAVLPVVEDEGEEPPPSDTF
                     AECDSDETISAVAAALGRRYEVIPIESDEDAYARLRNTRPDLVFNIAERLTGPNREGH
                     IPTLCEMLDLPYTGSDPLTLSLCLDKSRAKEILSYYGVPNPEFRVVEPGEAVPDGIKL
                     PAIVKPLYEGSSKGIKNNSYVATAEELAARVREVTGLYRQPVIIERFLTGREFTVGVL
                     GNVPNLEILPIVEIDHSLLPPEAHPIYSYEAKWIWDTPEKPLAIFKCPARISSGLEKE
                     IRTIVADTCRILRIRDWARVDVRLDEKGRPHILEVNPLPGILPNPEDNSCLPKAARTA
                     GYSYDELILRVVDEARRRWGLVSSRKGRS"
     gene            complement(31829..32620)
                     /locus_tag="PKV64_03310"
     CDS             complement(31829..32620)
                     /locus_tag="PKV64_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16142.1"
                     /translation="MDERDFPSKKRTLVLRDDQADFYAILDEARGRRRRGGRLHLIDA
                     GRLTVLELERICLSGADLFTSDKAGRAPADLAVLSAAAGKSGGEIAHFQHGPLLAEGE
                     GVVLSLRALRDAAGGGVSLYLSNKTEKRRTEDLAVLAEDARRGNRRLGYYHHGAPAEA
                     LEELARRGAWIHVSAAGAGADLPALLLLRDIAAKAAAAGAGLVIHLERLIPEPDLEDL
                     HKAGAFLLFHVPPSDYRSPYRALRAKASRRVLDPRAYYLYSEFMR"
     gene            32939..33640
                     /gene="queC"
                     /locus_tag="PKV64_03315"
     CDS             32939..33640
                     /gene="queC"
                     /locus_tag="PKV64_03315"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HPN16143.1"
                     /translation="MRKKTLVLFSGGLDSTTALHWALRRGGKVSALTFDYGQRHRVEV
                     RAARTIARRLGVPHKILKIDLEQIGGSALTEPSIAVPRHKRLPRLRTTAPPVTYVPFR
                     NGIFLALAAAWAEAQGINEIVCGFNVIDSPAYPDTRPVFVRAMERAVRAGTAAAAAGR
                     PVRIRAPFAGMKKSEIIRTGLALGADYSLSITCYSGKERPCGSCSACLLRARAFKEAG
                     CSDPLVLRTGKGELP"
     gene            33637..33984
                     /locus_tag="PKV64_03320"
     CDS             33637..33984
                     /locus_tag="PKV64_03320"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPN16144.1"
                     /translation="MSWTLKVRDRFSAAHFLREYQGKCEKIHGHTFQVEVEVDVSELD
                     AAGLGIDFAVLKAGLAEILPDHTLLNDVYPFNPSAENLARRFYEDLKPRFPVRAVTVW
                     ESEDARATYAEDR"
     gene            33953..34582
                     /locus_tag="PKV64_03325"
     CDS             33953..34582
                     /locus_tag="PKV64_03325"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HPN16145.1"
                     /translation="MPGRPTLKIAEIFAGIQGEGLRMGRPAVFVRLAGCNLRCSFCDT
                     KSAWRGGRLMTAAAVAAAVKKARRPWSGEWICLTGGEPFTQDFGPLAERLRAEGFRIQ
                     VETNGTIFKDIPLDWFTVSPKPPDYDAAPEFHARAREVKLVVSRELTLRAVGRVRAAF
                     PASVPVFLQPESNRPESRDRAIRLLRRASARGLGDVRLGVQLHRVFGLR"
     gene            complement(34552..35604)
                     /locus_tag="PKV64_03330"
     CDS             complement(34552..35604)
                     /locus_tag="PKV64_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPN16146.1"
                     /translation="MNALPPKNPSFGWSDVFMLLVTLIWGLNFSIIKISLRELSPAGF
                     NGLRLLLTAAVLVGLMAASGESFRVGRSEFWKLAALGIMSHALYQILFIGGLSRTTAS
                     NSSFILSFSPAIVALIGGLLRIEKVRWGAALGIVISAAGLYLVIAGGEGGIRLLSAGF
                     QGDVMIFAGTILWSLNTVFSKEFLERLSPLKFTAITVGLGALAYFPFCAGAISRISWR
                     SVSPAAWAGLGFSAVFSLVVGYLVWYVSVRRVGNIRTAVYSNLTPVFTALFALLILGE
                     ALRPRQIGGAAVILLGVFLARTEYGFFLGGRNGIRPAPVSRGSVAAAAAPDPPENAGR
                     PADGPAAQRRPKTRWS"
     gene            complement(35656..36072)
                     /gene="queF"
                     /locus_tag="PKV64_03335"
     CDS             complement(35656..36072)
                     /gene="queF"
                     /locus_tag="PKV64_03335"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HPN16147.1"
                     /translation="MSGYGKKEATAGLKTRLPSIDVFPNQFRDYEITIMVPEFTSVCP
                     KTGLPDFGTITITYVPRDSCLELKSLKEYFLAYRDLGIFYENAVNRILEDIVAAARPV
                     RAAVKGSFNPRGGLLSVIEAAYPRAQKDAGRAKKRP"
     gene            36137..37084
                     /locus_tag="PKV64_03340"
     CDS             36137..37084
                     /locus_tag="PKV64_03340"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPN16148.1"
                     /translation="MASPSFLYGPVPSRRLGYSLGVDLLPFKTCSMDCVYCQLGGGAR
                     TTVRRRAFIPVETVLAGIENVLRRGGRVDAITFSGSGEPTLHPGLGRLIRAIKRKTAV
                     PVVLLTNGSLFTRPSVRKDAAAADIVVPSLDAATEDAFRKINRPHPGLSAAAIIEGLA
                     AFRRHFHGRLWLEVLFVKGINDRPSDLRALKKAIARIRPDRVHLNTIVRPPAESWARP
                     LGRRDLEKIRAFLGETAEIIADFKKPGRRTRTKDLPSRILDFVRRRPETAERIAASLG
                     ASPEAVERAGRRLEKDGRLRLVRLNGRVFFEPGAAAGKK"
BASE COUNT         7123 a        11811 c        11343 g         7101 t
ORIGIN      
        1 ttcgggggtc agccgcttga tccgccggcc catcttctcg caatgcttac ggaggaaatg
       61 ctggatcagg agggggatgt catcgggtct ccggcggagg ggcggcatgt cgacggagat
      121 gacgttcagg cggtagaaga gctcctcgcg gaatttcccc tcttcgatcc gcttgcggag
      181 attctggttc gtcgcggcga tgatccggac gttcaccggg atttcctcgt ttcccccgac
      241 ccggcggatc atccgctcct ggaggacgcg gaaaagcttg acctgggtcc agggcgacgt
      301 ctccccgact tcgtcgagga aaatcgtccc ggcatgggcc gtttcaaaca tccccttctt
      361 gtccgagatc gcccccgtga aggatccttt cacgtgcccg aaaagctcgc tttccaggag
      421 attttccggc agggccgcgc agttgatcga aacaaaggcc cgatcgcgcc gccggctgtt
      481 ggcgtggatc gcccgggcgg ccagctcctt ccctgtcccg ctctccccgg tgatcaagac
      541 cgtggaatcc gtggccgcca ctttttcgat cagggtgaag acctcctgca tcggccggct
      601 cttgccgatc atgttctcga agctcgtctc ctcccggagc gcctgcttga gaatcttgtt
      661 ctcctcgagg agccggcggt gctcgaggcc gttggccacg aggaccttga tctcctcgat
      721 gtcgaagggc ttcatgatgt aatccatggc gcccagccgc agggcttcga cggcttgctt
      781 gacgctggcg taggccgtga tcatgatgac cggaacggtg ggcttttccg ccttgacccg
      841 ccggagaacc tccattccat cgacctgcgg catcttgatg tcgctgatca ccaagtcgaa
      901 ttccttctgc tcgatgtcct cgtaaatgct gggcgttccg ggattgacga ggacggcgta
      961 tccttccttc ttgaggatga cgctcagcag ctccaggatc gagcgttcgt cgtcgacgat
     1021 caggatcgtg ttcatcggca atccgcggcc ccggagttgc ctttcgcgcc gaattcacgg
     1081 tagggcagaa tgatcttgat gtccgttccg acgttgggct cggattggac ctggatcttg
     1141 ccggcgtagc tctcgatgat ctgccggacg acggacatgc ccaggccgcg gccgccctcg
     1201 aaccgcgtgt aaaacggctc gaacatgcgg gctttttccg cggccgtcat gccccgcccc
     1261 gtatcgacga tccggatggc caggacgttt ttgtccggac ggaaaaaatc gatcgacagc
     1321 ccccccccgt cgggcatcgc cttgagggcg tttccgagga gattccagaa aacctgcttg
     1381 aactgcccgg gactgccgaa atacaagaac ggcgaggctt cgtaattccc cccgacggcg
     1441 atccggtcgg acatttctcc gctcatccgg agcatcgtca gggtctcgac caacggatcg
     1501 gccagccgga acaccgagta cgtctgcttt cccgggctgg ccaggcagag aaactgctcg
     1561 atggactggg agacgcggtg cgattccttg accacgatat ccatcaagcg ggcttgttcc
     1621 ccgccgagat ccaagtcgtt ctgcaaaacc tggaccgccc cggaaatcgc cgccagcgga
     1681 ttccggatct cgtgggcgat gaacgccgaa gcccgcccgg ccgccgcgtc ccgctcccgg
     1741 atgttgattt cctgctgggc cgcgtccagc tcggcccgcg ttttttggag gctcgaggac
     1801 agtttggcga tcaagacggc gatgacgccg aacagccccc aggccatgaa caccgtgtag
     1861 agcacctggc cgatctcgat ttccgctccc tggtcgggcc ggaaatagag aatccagccg
     1921 tacgccaggc tgtcggcaac ggccccgaaa acgatcgctg aaaaggccgc ggtcatcaag
     1981 gccgcccggc ctcccaggac caagccggcg gcgatgatcg cgaagacata gagaaaataa
     2041 agctggccgc cgacgccccc cgagatatag accagtccgg tgatgaccgc gaggtcgatc
     2101 aggatctggg cgtaggcttg ggcccggtag cgcgtgttga acagaagcag caggaaaaac
     2161 acgagacccg tggcgtactc ggtcacgatc aggaaataga acggggacag cggcaaaaaa
     2221 gaggccgtgg ccagctgaat cacgaccgcc gcgacgaaca gcgacgtgat cacgacgagc
     2281 cgccagatga gaagccagcg gacgttggac ttgagggtcc tcatcgccgg attattgaat
     2341 ctggcccatc aggctgaaga tcggcagata catcgagatg atcaacgtcc cgacgaggcc
     2401 gccgatgaag accatcatga acggctcgag gacggagaga agcgttccga ccgtgttgtc
     2461 gacttcgctg tcgtagaagt cggccagctt ggtgagcatc ttgtccaggg tgcccgtcgc
     2521 ttcgccgacg ctcaccatct gggtcatcat gaacgggaac cggccggtgc ttttgatcga
     2581 atcggtcaga cttcggccct cggtcacctg ttttcggatg tccatgacgc tcttttccag
     2641 gatgacgttg ccggccgtgg tcgccgtgat cttgatgctc tcgagcatcg acacgccgcc
     2701 gcccaggaga gtgctcagcg tccgggtgat ccgggagatc gcgactttcg tcaacagcga
     2761 tccgaacagg ggcaagcgaa ggagaaggcc gtcggtcgtc caccgccccc cctgggtttt
     2821 ccggtagtag cggaagagaa agagggctcc gaccgtcccc aggaccagga agaggatgta
     2881 cttctggacg aattcggaga ggttcatgat gaacgccgtc agccccggca gctgggcgcc
     2941 caggtcgacg aatatttggg cgaacgtcgg gatgaccttc cacaacatga aaaccgcgac
     3001 gataatggca aaggcgaaaa tcgccacggg ataggtcatc gcctgcttga ccttagcctt
     3061 cagcttgacc gagttttcga tgtaatcggc cagccgctga agcatcgtgt ccaaggttcc
     3121 gctctgctcg cccgaggcga tcaggttaca gaagagatcg tcgaagactt tcggaaactt
     3181 gcgaagagcc tggtccaggc tcgaccctgc ctcgacttcc gcccggacct gggtgatgac
     3241 ccgcttgaaa taggcattgc gggtctgttc ggccatgatg ttcaagccct ggatcagggg
     3301 aagctcggcg tcgatcagga cggccatttg gcgggcgaaa atcgccaggt ccttctgctt
     3361 gactttctcc cctttcaaga acggcaggtt gaatttcccc cggaccgggg agatcttctc
     3421 gaccttgatc tgttcccgtt ggagaaggcg ggccaactcc tcggcgttgg cggccacccg
     3481 ctccccctcc actccgtccc cgaacttgtt ggtgcctttc caaatataaa ccggcatcgt
     3541 ttccctccgc ggtcagcgtt tgaccgccac tcccacgccc gccgcccgcc gctggatcag
     3601 gtcggtcagc tcctcgggat tcgacgtggc ggacatggcc gtctcgagcg tgatgacttt
     3661 ccggcaatgg agatcgcaga ggttctggtt gaacgtcagc atgccgaact tttcctggcc
     3721 aagctgcatc tgggaataga tctgatggat cttgttttcc cggatcaggt tgcgaacggc
     3781 cggattcgga atgaggatct ccatcgccag gacccgccct gttccatccg cccggggcag
     3841 gagcgactgg cacatgacgc cttccaggga catcgagagc atgacccgcg cctgggcttg
     3901 gtactggggg gggaagatgt cgatgatccg ggagatcgtc tccggggccg agttcgtgtg
     3961 cagagtggaa aaagtcaagt ggcccgtctc ggcgacccgg agagtggcct cgacggtttc
     4021 ctgatcgcgc atctcgccga ccagaaccac gtcgggatcc tcccgaagga ccgagcgaag
     4081 ggcgttggag taggagaggg tgtcggacaa aagctcgcgc tgattgatga gcgccttttt
     4141 cggggtgaag aagtattcga tcgggtcctc gatggtgatg atgtggaccg cccgctcggt
     4201 gttgatgtgg tcgatcatgc aagccagggt cgtcgacttg ccgctcccgg tcgggccggt
     4261 aatcaaaatc agtccgcggg gcagaaagca gagctgggcg atgcgggaag ggatgccgag
     4321 ccgctgaaaa ctccacatcg acgggaggaa gcgccggatg gccgcggcca cgctgccctt
     4381 ctgcatgaac acattcgccc gaaaccggcc cagatccttg acgccgaagg agaaatcgag
     4441 ttccattttc tcctcgaaca gctttttttg cttgtccgtc aggatgctgt agacgagctg
     4501 cttcgtctcc gtggcggtga ggggcggatg ggagatggac ttcaattcgc cgtggacccg
     4561 gagacgggga gggaccgaag tcgtcaggtg caggtcgctg gcgtcggcat cgaccgccat
     4621 cttgagaagc tcatgcatcg aaagcggcat cgaatggctc ctaatctcgc acggtttcgc
     4681 gaaggacttc ttccggcgtg gtgatgccgg ccatgatctt tatcaatccg ctcctccgca
     4741 tcgtgatcat gccggcctcc acagcttttt gcttgatctc cttggattgg gctcgaagaa
     4801 ggatcatttc ccggagggtc ggattgagtt ccatgacttc gaacagagcg gtccggccct
     4861 tgtagcccga attatggcag atatcgcacc cgcggccctg catgaccgcc gccttttcgg
     4921 cttcctcgga ggtgaagccc atctcgacca gcgatttgac cggcagcttc gtcggagcgg
     4981 cgcatttctt gcagacgcgg cggaccaagc gctgggcgac gacgagagcg agcgagtcgg
     5041 cgatgaggaa cggctcgata ttcatgttca tcagccggtg aatcgtggcc gccgagtcgt
     5101 tcgtatgaat ggtggaaaag acgaggtggc cggtcagggc ggccttgatg gccacgtcga
     5161 cggtttcctg gtcgcgcatc tcgccgatca gcatgatgtc cggatcctgg cggaggaagg
     5221 cccggagggc ggcggcgaaa ttcaggttga tctcgtcccg gatgttgacc tggttgatgc
     5281 cggggatgta aaactcgacc ggatcctcgg ccgtcatgat gttcttttcc atcccgttga
     5341 gcgtcgagat ggccgaatag agggtgttgg ttttcccgct tccggtgggg cccgagacca
     5401 ggatgatgcc ccacggccgg gcgatggccc gctggaagac ggccagcgac tcggactcga
     5461 agccgagctt gttcaggtcg atgttcaagt tgtcccggtc gaggattcgg gcgacgattt
     5521 tttccccgaa aatcgtcggc aggcaggaaa tccgcatgtc gacttctttc ttcaccgcct
     5581 tgtccagctt ggcccgcatc ttgatccggc cgtcctgggg caggcgcttt tcggcgatgt
     5641 ccatgttgga catgatcttg acccgggagg tgagcgggtt cttgaacttc aagggcaggt
     5701 tcatggtttc atagagaatg ccgtcgatcc ggtaccggac gcggaaagac ttctcataag
     5761 gctcgaagtg aacgtcgctg gcgcctttct tgatggcttc gacgaagatg ttgttgacca
     5821 gggtgatgat cggcgcctcg tccggaacct cctcgatctc ctcctcgtcg ggctcgtcct
     5881 tgatctcctc gagctgatcg atggcgctga ttttcgaatc gtccgccagc tcgatttcct
     5941 cgatgttctt cttcacccgg agggccgtgc ccgcgccgta gtacttgttg atggcgttga
     6001 ggatgctgct ttcggaagcg atgaccggct ggatgctcag ccccgtccgg aaacggatgt
     6061 cctcgatgac gtccatgtcg gtcggatcga ccatggccag ggtcaggaat gatcgaatcc
     6121 ggtggatcgg catgatcagc gatttcttgg ccgtatcggc cgtgatcaag ctgatgacat
     6181 cgggatagac ctcgaactgg tccaggtcga tgtagggata tccgagctgc cggctcaatc
     6241 cctgggccat ttcctcgccg ctgacgtaat tgagagagac gatggccgag ctcaggagaa
     6301 tgtcgtgttt tttctggaaa tcctgggcgg ttttcagctg ttcagccgtc aggatctttt
     6361 ccttcagaag aacgtcgccg attgtcgagg ccattggcaa cctcagtgta tcatgtttat
     6421 ccgtattgac aaataatgtc aatcgccggg ggcgcggccc tcccttttat agaaagacgc
     6481 ttttcacccc ccaagattat cgtctgttta cccgttgcag gtcaatcgtt cagccggcgg
     6541 gagaaaaagc cggatccccg ccccgctttg cccaatccgg ccgaccctct ccggccggtt
     6601 cgtcaatccc cccgggggaa ggagggcgcg acggggcttt ttttcctcca ggccttgcgg
     6661 accaacaggc agatcgaaag aatgatcaag gccatgagaa gacgggcggc ccaaagatag
     6721 gggcgccgct cggccggttc tcccttcatc aggaatttgc cgacggcgtc ctgccaggtc
     6781 caaacgccga ggataaccag catgtaagcg ggggtgatgt acttcaggat gaatttgaag
     6841 acgcccggga ttttgaaatc cgcgcccttg tgcatctcct cccagccgtt cttgaggccg
     6901 aagatccagg agaacacgat gatctcgagc gctccgaaca cgacgatgcc gaacgtgctg
     6961 atccagaagt cgaactcatc caggacgccg aaccggaaga aaaagactac gaaaaggacg
     7021 gccccgatca ggaacagaaa gctgcggttg accgccttct tccgggaaat atggaattcc
     7081 tcggtcaaga aggccaccag cggttggaga aaagccaccg atgtcgtgat gccggcgatg
     7141 aaaagaagga ggaaccacat cgccccgaac aactggccga acgggatctt ctggaagatg
     7201 aggggaagag agacgaatcc caggtcgaag gccccgcccc gggcgataac ctcggtttgg
     7261 gccaggccga aaaaggcgac ggccacgggg atggcgatcg tcccccccag gacgacctcg
     7321 gcgaactcgt tgaccgtcga cgtggccaag ccgttgaggg tgatgtcctg gttttcccgg
     7381 acataggagg catacgtcag gatcatcccc tggcccaggc tcagggtgaa aaaaatctgg
     7441 ccggccgcga ccagccagac cgagccctgg gtcaagcggc tgaaatccgg attccacata
     7501 aaacccatgc cgttggcgat gttccaatcg ggatgggccg gatcgggagt gccgagcgtc
     7561 aggacgcgga tgacgagaat gacgccgaag aggaaaagca ggggcatgcc gtggcgggcc
     7621 aggacctcga ttcccttgga gatgccccgg ctgagaaaga agtaattcat cgccaagcag
     7681 acggccagaa agagtaaggc ggggatgagc gaggaaaaat accggttcga ctcaacgccc
     7741 tgaaatcctt ttagaaagga ggacatcgcc tcttgattga ccagcccgaa atatcgtccc
     7801 gtcgccgaaa accaagcata gcccaaacac caagccatga tgaacaagta ataaacggcc
     7861 accgcgaacg ggatgaaaat gccgaacgcc ccgatgtact tggagaacgg atgcttccac
     7921 atcacctgga acatccccgg gcccgagccg tggccgtggg ctccgccgtg ccggccgatc
     7981 gtccactcga cccacatcat cggaacgccc atcaggatga gggcgcagaa gtacggaatc
     8041 aggaaagccc cgccgccgtt ggaggcggcc ttggccggaa agcggagaaa attgcccaag
     8101 ccgatggcgt ttccagccat ggccagaacc agtccgatgc gggtggccca gcgatcgggc
     8161 cgcccgcccg gccccgtttc cacgaccgct ttcttcttgg tgaaaagaac acgggacatg
     8221 caataaacgc ttaagccgag gatgacgccc cagccgcaaa tgaaggagat ccagcccggg
     8281 agagagaggc cttgattcat gtcgccctcc ggtccaacgg cgtttcggcg gacatcataa
     8341 tcccaatggg gaaaacgtat accaaagcgc cgggccgttg tcaaagcggc cggggaaaac
     8401 cgcgcctctt tatttttcct ttacttatct aattatttcc tttacaatcc ggacgaatta
     8461 ggcgtttttg ggtcgagatt taaagacggc ttttcgacgg aaaaggtcaa tttgaggacc
     8521 ctgaagaaaa cgattgctct ccgcccgtcc atcgatcccc gctttccgtt ccctcgctca
     8581 aacaagaaca ccgaaccggc cgtcctcggg ctgtttattc tttctcacag gagcgccttg
     8641 aatgtctgaa aaaacaccgc cccccgatcc gatacgcccc cgaagccctc tcccgaaaga
     8701 caatcgaaca ggaaacgacc ggcgcggaag cgaccgccgc cgatctcaac accaggccgt
     8761 tgaatcggag cgccggatcg gcgaccggcg gaagcacgac cggcgcgtgg aaaaaacgtc
     8821 tccctatcac aacttggtcc ggttcttgaa ggaaaaggga ttatatcccc acgaaaaagc
     8881 ctacgaccag ccggacgggg ccgaggtcgt ttttcggggg aaaagcatcc tcagcctggc
     8941 ctcggtcgat tatttcaatt tcgcccagaa cccggaaatc aaaaagaccg cctgcgaggc
     9001 gatccgcaaa tacggcgccg gtggattttc ctcccgcctc tccatgggaa acttgagcat
     9061 ccacatggaa ctggaagggg cgttggccga attcatgggc aaggaaagcg tcctgatctt
     9121 caactccggc tatctggcca acttggccct catcacctcg cttctccccc ggaacgcgac
     9181 gattttcctc gaccagaaat gccatttgag catccaccac gcctgcctca tgtcgcagaa
     9241 aaaatatttc cggttttcca acaacgacat gagcgatctg gagcggctgc ttcagaaaca
     9301 ccgggacgag ccggaaaagt ggatcgtcac tctgggcgtt ttcagctcca acggtctttt
     9361 ggcgaagctc gacgaaatcg ccgtcctggc ccggaagtac aaagcccgca ttttcatcga
     9421 cgacgcccac gggatcggcg tgtatggcga ccggctgcgc ggcgcggccg atcatttcgg
     9481 cgtgctggac gagatggact tgatcatgtg cccgttccag atggccttcg ggaacatcgg
     9541 ggcgttcgtc gtcggcaagc gctatctgct ccaggccgcc cacaccgacg cgtggcccta
     9601 catgttcacg tacaacatcc cgccggtcag cgcggccagc ctgctgaagg cgctcgagat
     9661 cctcggccgc gaaggccggg agtatcagaa aaagctctgg gaaaatgtcg cttacctccg
     9721 ccgggctttg acggagatcg ggtacaagat cctcaatccg aacggacaca tcatcccgat
     9781 tttgatcgga gacgacgtga aagtctgcgc cttcgcccag cggctgttcc atcaaggcgt
     9841 ttgggttcag ccgttttatt atccggcggt ccccaagggc caggccaccg ttcgggtcac
     9901 ctgcacggcc ggccacaccc ggtcggagct ggatcacgcg atccgcgcct tcgccgatgc
     9961 ctctctctgg ctcgcgaact cctgaactcc atgccgctca ccgcttttct ctccgcgttg
    10021 ctatgtctct tgaccggcgt tttcgtctat gtcaagggga aaaacaatcc ggtcaaccgg
    10081 tatttccttg ttttcaacat tctgattttt ttctggaaca gcggcgactt cgtcattccc
    10141 ctgatgggcc cgcggatatc cgccggattg atctacgacc gcctctccca gatcgggggg
    10201 atcctgatcg ccccctggtt cattcttttc tatttgacgt tgacgggcct gagcttcagg
    10261 gaaatcgtcc ggacgaagat cttccgcgtt ttcgtcgccg tcgccgtcct ccttcttttt
    10321 ctggcgccga cgccgctctt gatcgaaaga atccaggtgg agccgttcaa agaatttccg
    10381 ggcaaactcc tttacgtgga aatcacggcc gtgttggcga gtatcttttt cggcttgagg
    10441 cacttgttca tcgcccggaa aaccgtcaac ctggcccaac gagtccgtct gacgtatttc
    10501 ggcgtcgccg tgcttatggg gattctctcg gcggtcgctt ggtttgtcat catcacgttc
    10561 gtccccggct tgtccacgaa attcatctac gtgtttgaag tcacctacgt tctgttgacc
    10621 gcctacgcga tcctccggca ccacttgatg aacatcgacg tcattgtcaa gcggaccgcc
    10681 ctgtatacga ttctcagcac gttcgtcatc ggcgtctaca tcgccatcat cctgattttc
    10741 gaggtcgtgt tccggtcgct taccggatac tcctccctgg cggaaaaaat catcgccgcc
    10801 ttcatcatcg ctctcacttt tcaacccgtg cggacgctca tcgaaaaaac aatcgaccgg
    10861 atttttgccc ggggaaaggt cgattaccag gacgccctgg ccaaattcac ccgctccctc
    10921 ggttccgtcc tcgatctccg ggagctggtc gatctgctcg tctccatctc gggcatcctg
    10981 agggccaaga acctggccgt catgctccgg gacgaacagc gcggacggtt caagatcgtc
    11041 gcctccaccg gattggactc tccccttcat aagatcggat tcgatgattt caacgacatc
    11101 gtccgggtgc tgaacacaac caataaaatc atcacgaccg acgacatcaa gcggctacgg
    11161 tcggaaaacg tctacaagaa cctcccgggc gaagtgtccg ccctccgggc cgaggtcatc
    11221 cttccgatct tcgtcaaagg ggaattggac ggcatcctgt tcctggggga aaaacggtcc
    11281 gaggagcggt acgaccgaaa cgatttcagc gtgctggcca ccttggccga cgaagccggc
    11341 atcgccatgg agaacgcgaa gctttacggc agcgtgaaac ggacatactt cgaaaccgtc
    11401 caggccctgt ccctggcgat cgaggccaac gatgaataca cccggggcca ttccgaccgg
    11461 gtcacgaaaa tcgccgtcaa aatcgccaag cgcatccggc tgacccggga acggatcgac
    11521 accttgaagt tcgccgggat ccttcacgac atcgggaaaa tcggcgtcat caaggaagtc
    11581 ctgcacaagc ccacgaagct gagcgatctc gaattcgccc tgatcaaaaa ccacccggtc
    11641 gtgggcgagg aaatcatcaa gccggtggct ttcctggaca agatccggcc ggtcatccac
    11701 catcatcacg agcgattcga cggaagcgga tatccggacg gcctccgggg gaaagacatt
    11761 cccttccttt cccggatcat caccgtggcc gacgcctacg acgccatgac cagccaccgg
    11821 ccttatcgca gcgccttgag ccacgagacc gccgtcgcgg aaattacaaa atacgccggg
    11881 acgcagtttg atccggagat cgtccaggcc ttcctggaaa tcgccgggac gttgatttga
    11941 tccgtcaggc cggcgggccg gccgcggcct ttctctcgcg agagacgccg agcttcggcc
    12001 ttgccgcgca accgatcaga acggcgtctc attcgattga cctcccccgc gacattggct
    12061 atcatgaaac cgcctccggg agaagggaac catgaacatc ggggctttcg ggcgtgtgat
    12121 cgccggtgtg ttgctgttgg cggcggccgc cggcgcttat cttcgtccga agatcggatg
    12181 cgatgcgagc tcgattcatt gccacacgaa tccctcggcc gggccccagg ccacgattat
    12241 ccgcatcttc aacaacggcg gcgcgacgct gagctggcgg atctccggcg gcgcgccctg
    12301 gctcaagatc agtccccttt caggcgtgaa tgacggcacc gtgaccgtca ccgtcgatcc
    12361 cgccggcctt gagccgggga gatacgaaag ctcgttcatg atcaaggcca agttcgcggt
    12421 caactcgcct ctgaagatca aagtgatcct cgacgtcgcg accgaccgtc tcctggccgg
    12481 attccgggcc tcgcgctacg gggtcgatcc cttcccggac cccgcgtatt gggcgtccgt
    12541 cggcgcggga atggcttccc gctttcccgg cgccggcatg ggatgcgtct ggatcgtcgg
    12601 cgtcttcctc gacaacggcg attgcgggtt gaactttcct tctccgggcg gcgattatcc
    12661 ctctattcat ttcctcgacg aagaccagaa cgaagccgcc ttgagcttct ttgatgccct
    12721 ggatctctcg gtttggctcc aggtcgagcc cggccgggcc tcggtcccga agctcatcga
    12781 cctcgttctc cgccgctacg gccatcatcc ctgcgtcaag ggcgtcggcg tggacgtcga
    12841 atggtacaaa acggattcgg cccaccccga gggcaggcgc gtgaacgacc tgagcgcctt
    12901 gctctggagt ctccgggtcc gatcctataa cccggcctat acccttttcc tgaagcactg
    12961 gcttatcgac aaaatgccgc cccgctttcg gctcgggctc tggttcatca atgacagcca
    13021 gcaattcggc tcattgggcc agatggcgag tgaattcaag gcctggggcg agcatttctg
    13081 gccgagctcg gttggattcc agttcggata cgaggccgat aagccttggt ggagcttgct
    13141 ggccgacccg ccccggaata tcggggatgc catccgggcg gtcgtgccca acgctttcgg
    13201 tcttttctgg gttgatttta cgatccagga catctttccc tgacgaagca agctccgcga
    13261 tgaaggcatt cttcaaaaaa aaacgcgttc tcgtcacggg ggggacgggt catctcggaa
    13321 gcgccttgat ccatcacctt gtccaaaacc tggccatgga cccggccgcc atccgcgtat
    13381 tctatctcca aggttcgacc acccggtcgc tccgcgacat ccccggcttg gattacgcgc
    13441 cggggaacat cctcaacccg gacgacgtcg agcgggcctg cgcggggatc gattatgtct
    13501 ttcatgcggc cgcctcgacc tcgtttgatc cgcggagcaa gaggcttcaa tggatgatca
    13561 acgtagaggg gacacgcaac cttttggagt ccgtccggcg atcgccgacc atcaagaaaa
    13621 cgtcctttac gagcaccgtc aacgtcctcg gcgttccgaa cccggccgga tccgccggca
    13681 acttcgagaa ttccgatccc tacaccaacc ggccgaggct gcacgctttt cgttcccggg
    13741 aggagactct gtcgtttatc gacatggtcc gcgagcgccg gtcatcacct tgggaaaaga
    13801 ggatcggaat cggctacttc gattccaagc tggccgccca ggagctcgtg aaccgctacg
    13861 tccgtgacca cggcctcaac gtcgtctcgg ttcttccggg gacgtgcttc ggcccttacg
    13921 acgttctcgg cggcaacggg atttacctcc tccggatcta ccacggcgcc ttgccgggga
    13981 tcctcaaggg cggcttttcc gcgactcacg tgatggatgt cgtcgaaggc catctcctgt
    14041 gcctggtctc ggcggccgcc ggcagcgctt acatcatcaa cggcccgagg aaggacgatc
    14101 tccattttac ggacgtcgcc cggaccatcg ccgaggttct cggccggcgg tatcccgaga
    14161 aaaaaatcaa gcggccctcg atgatcgttc cctcggggcc ggcctgggcg gcggcccgcg
    14221 cttccgagct cgtcgccgcg gtccggagaa ggccctgcct cctcagccgg gccatggtta
    14281 aggccggctc ccagcctctt ttctacacgc atgaaaacgc cgaacgcgat cttggatatc
    14341 gtcccaaacg caccttccgg caggccgtcg aggaaatggc cgcttacttc gaaaaggaag
    14401 gattgttctc cgccttaacg ccccgacctt tcccggccgg gccgcccgag acgggggaat
    14461 tcccggggtt ctgaatccac cgtaaccaag gttcgtccgg cgtccgtgcc tcagagagtt
    14521 ttaacgccgg tccgcggcgc gggacgtcga aacgggaggc ctactcgatc gatccttggc
    14581 gcggagcggg cgcgaaacgg cctcttatcg cggccgttgc ggcgctccgc gaatccaaaa
    14641 aaccggcgga atttccgcgg aataaaaagc gcgcctggag agactcgaac tcccgacccg
    14701 ctgcttagaa ggcagctgct ctatccacct gagctacagg cgcgccggac cggcgcttga
    14761 ggccattccg aaaaaaaatc ggggaggacg gattcgaacc gtcgacctct tgctcccaag
    14821 gcaagcgcgc taaccaggct gcgccactcc ccgcgcgaag gaagacgcgg ccctttttcc
    14881 gggacggggc gcgtgtcccc gatttttata gcagatagcc cgggcatcgt caaaccgggg
    14941 cccttgatga aaagacgcgc ttccggggaa agaaaacggg ttcggatcgg gagtctccgc
    15001 ccggcgtttc gcctgctttc ttcacaagcc gggatgcctg cgtctccctc aagcccttcg
    15061 caacgaagca gaggcggttc catcggcttg cccgaaaggt aaatcatcct attttccttt
    15121 taataaaata aaagcgagaa agaagaatct taaaagattt ataaatcgtg cgggttagga
    15181 ggcgggatcc gagaccggcc gtcgaaggcc gcgctcgatc tgggagcggg ggatgcggac
    15241 gacgatgggt cggccgtggg ggcagacgcc gggattggcc gaccggaaaa gctctcgaac
    15301 gaggaattcc attttttcgc gcggcaggta ttcccccgct ttgaccgccg acttgcaggc
    15361 catcgccgcc aggagccggt ccttggccgg cgcgggcgcc cctttcttgg attcgtcggc
    15421 ggcggccagc acaacggaca aggcttcctc cggccggaaa acatcgggat actcgcggag
    15481 ggcgtaactc cgcccgccca tcggttcgac ccggaagccc gaggagcgaa gcatcggccc
    15541 ggcttcctcc aggccgattt cctgaaccgg ggaaagctcg aaaacgaggg gaaggaggct
    15601 cattttaacc ggccatttcc tttcccggtc gatttcaacg taacggtcat agatcaccct
    15661 ttcgtgggcg ttgtgctggt cgatgatgag aagatcgccg gcctcctcgg ccaaaatgta
    15721 ggccgatcca agctgtccca ggacttgggg ggattccgcc gcccgcggaa ggactccggc
    15781 ggcgggaaaa tccgcgaaat ccgttccccg gatgtatgtc cggcccccct cgccgacgcc
    15841 ggaacattcg ggctcctccc tttccggccc ggcgccctct ccattctccg ttccggcggg
    15901 ggccggcctt ccgcccggcg ggactccggc cccgtccgcc aggatctcgg aaagatccct
    15961 cacccccaga acccgggtcc gggccctctc gaccaccctc cgaagcaggg ggaaaaagag
    16021 ctgggcgtcc ctgaaccgga cctcggtttt ggccggatgg acattgacgt cgacttcgcc
    16081 cggaggaatg tcgacgaaga gaaacgcctc cgggaatagc cctttttcca gaagtccgga
    16141 gaaggcctgt tggagagcgg ccgaaaggat cttgtcccgc accggccggc ggttgacgaa
    16201 aaaagcctgg cggccgcggt tcgggcgtcc gtacggcgga cgagagacgt aacctgaaac
    16261 tcgaaccgga ccgtcggcca ggtccgcctc catcatctta tcgagagcgt ccttgccgaa
    16321 gagctgaaaa agccgctccc ggaggccggc cgccggcggg caatccagga cggtccgggc
    16381 gccatgacgg acaatcaggc gcagccccgg acgggccaaa gcgatgttcg taagcatctt
    16441 gacgatcaac ccaagctcgg aggaatcgct cctcaggaat tttttccggg cgggaaggtt
    16501 ggcaaagaga tccctgacct cgacttccgt cccccgcggg aaagccgaat cccggacctg
    16561 gaaggcgccg ccgggctcgc tctcgacaag ggtgccgggc tcgtcgcctc cctcggaggt
    16621 gcggagagtc agccgggaga cggcggcgat ggaagccaga gcttctcccc ggaacccgag
    16681 ggtagcgatg gcgaacagat cgtcttcctt ggaaatcttg ctcgtggcgt ggcggacgag
    16741 agccaaggcc gcgtcctccc ggctcatccc gcagccgtcg tctcggacgc ggagaagcga
    16801 tttccccccg gccgcgaggt cgacggccac ggccgaagcc cccgcgtcca gggcgttttc
    16861 gacaagctcc ttgaccacgg acaccggccg ttcgatcacc tcgccggcgg cgattttccg
    16921 ggcgagggca tccggcagga cggcgatgcg ggtcatgagc ggaagccgtc ccggcctcgg
    16981 agttctccgg tcgtgtcccg ctcgccggcg gcggcgatgc ggacgtggac cggcgatccc
    17041 aagggcgcgg ccgccccgga gacaagcacg tccacgtagt tggacgtcag gacctccaat
    17101 ccgcccggtt tcgactggat gacgacgccg ggaagcgtcc gtccgatcag ccggcgccga
    17161 taggcgaggt tttttcttcc ggacaggcgg cgcagccggt cggcccggga tttgaccgtc
    17221 gcccgatcca cggccggccg ttccgcggct ttggtcccgg ggcgggcgga ataggagaaa
    17281 acgtgggcat aagccaaagg ggattcctcg agcagccgga ccgtctcctc gtagtcgcgt
    17341 tcggtctcgc ccggaaaacc gacgatgata tccgccccga ggttcgcttc cggggatcgc
    17401 cgctcgagct cggcgcaaag ttcgcgatag gacgcgttcg tgctctcgcg tcccatcaac
    17461 cgcagaatcc ggtccgcccc gtgctggagg gacaggtgga agtgcggcgc gatccgccgc
    17521 gatccggtca ggaaatccag gagggggcgg gaaaggagcc ggggatcaag cgagctcagg
    17581 cgaaggcgga aatcccccgg aagctcctcg attccggaaa gaagctcaag gagagaggac
    17641 ggcggccgga ggtcccgccc ataagaacaa aggtgaattc cggccaggac gatttccgca
    17701 tacccggcgg agacaaactc ctcgacgcgc gtccaaaggt ccaggaccgg gacgctgcgg
    17761 ctccgccccc gcacggaggg aatgacgcag aacgcgcaag ccatatcgca gccgtcctgg
    17821 gctttgacca gcgcccggac gcgataagaa accggctctt cggcccggac cgccgtctcc
    17881 ggcgactccc cggcggcgca ctgtccggca agcgcatcct tgtccgcgtt ggcgacgact
    17941 cgccaaacgc cgggcagagc tttcacgccc tccggatcgc gctcggccag gcagccggtg
    18001 acgacgagac gcgcccgggg attctcctga gcgattttgc ggatgtaccg gcgcccgtcc
    18061 cggtcggccc gggcggtcag ggtgcaggtg ttgatgataa cgatctcgcc ctgcgaatcc
    18121 tcttcggcca gccgccagcc tttcttctgg agcgccgccg cccagtcgaa cgcctcggcc
    18181 tgattgacgc ggcagccgaa cggatggatg aaaaacgagg tcatagactc tggcgggctt
    18241 ttttcgaatc ggcttcgacc tggacggcca ttccggcttt gtgggcggcg agccgagttc
    18301 ggaggtccgg gctcttcagg gcgagaatct ggacggccag cagggccgcg ttggccgcgc
    18361 ccgattttcc cgttcccatc gtcgccacgg gaatgccctt gggcatttgg accgtggaga
    18421 gaagcgcgtc gagcccgcca agccccccgc tttcgaccgg aacgccgatg accggacgga
    18481 tcgtatgggc ggcgacgatc ccggccaagt gggcggcctt tccggcgacg gcgataaaaa
    18541 cctcggcccc gccgtcctcg aagcggcgga tgagctcgat cgtcctctcc ggcgaacggt
    18601 gggcggacgt gacctccaga acatgcgtca cgccgaattg cttcaggatc tcgagcgctt
    18661 ccccggcgac gtccaggtcc gacgcggagc cgatcaagat cacgacatcc atgattctcc
    18721 tcctctcaag ccccggccgg attacggggc ggattcgaac gccccgatgt cgcgccggta
    18781 gtgcatcccc tcgaacgaaa tcttttcgac ggcccggtaa atccggttca tcgtttccga
    18841 cagacgcggt tcggcggcgc agacgcccag cacccggccg ccttcggtcc gataccggcc
    18901 tttttcgaac ttcgtgccgg catggaaaac gaccgctccc aggcgatcgg cgtcctccag
    18961 cccctggatg gtcttgcctt tctcatagga tccgggatac cctcccgaag ccaggacgac
    19021 gcagcccgcg gcccggcggt tccaggtcac ttcgtcggcc aggatatcct cggcgatgga
    19081 agccataagg agatcgacga aatcgctttc gagacggagc atctggggct gcgtttccgg
    19141 gtcgccgaac cggcaattgt attccaagac cttgggtccg tcggccgtca gcatcaggcc
    19201 ggcgtagaga gcgcccttgt atttccgccc ttcctcgagc atccgggtga ccgtcgggag
    19261 gatgatcgtc gacatgattc gggcgtacat cttgcggtcc aagaagggcg agggggaaat
    19321 cgaccccatc cctcccgtgt tcggcccctt tccgccgtca aagacggttt tgtggtccat
    19381 cgtcgtcacc aggggaatag ctttggcccc gtcggcgatc acgatgaaag aaacctcgcg
    19441 gccccggaga aactcctcga tcaggatccg ccctccggcg ccgccgaacc gcttctcgac
    19501 catgatctcc ttcaccccgg cctcggcttc ttcctcattg cggcagacca ggacaccctt
    19561 gccggcggcc aggccgtcgg ctttgagaac gaccggaaag ccgaattccc ccccggccag
    19621 cacggcccgg gccgattccg gggaatcggc gatccggaac ttccccgtcg ggatgcggtg
    19681 ccggtccatg aactgcttgg cgaagacctt gctggcctcc agctcgaccg ccttcttgga
    19741 cgggccgaaa atgcggaggc cgcgcttctc gaattcatcg acgatcccca gcgagagggg
    19801 gatttccggg ccgacgaccg tcaggtcgat ctcctccttg accgcgaaat cggccagcgc
    19861 cgggatatcg gtttccgaga tgggaacatt ttcggccacc agccgggttc cgccgttgcc
    19921 cggagcgcag aagactttcg agatcttcgg gctcttggcg attttccaag ccagcgcgtg
    19981 ttcccggccg cccgacccga ccaccaaggc tttcatcgtt ccgctccttc ccgaagggac
    20041 ggcttaagag acgcgcccga cggcctcggc gaacgcccgg ccgatgtttg aggccggcat
    20101 cgccgcggcg aagacgcggg cggccaaggg cacggcttcc gcggccggca gacagtactt
    20161 catctcggag aacttccggc ggaacgccgc gacgtcgtcc ttgacccggc ggttgcggat
    20221 gacgcagtcg gcgatcaagc cggcgacgac gtcgaaatcc ttccccgtca ttccgaaccg
    20281 ggtcatttcc tggaccccca tccggatgcc gctcgaaacg aggaacgtct cgtcgtcggg
    20341 gagggcctgg tagttcgtca cgatgttgtt gtcctccagc cggcgggcga tctccatgcc
    20401 gggaccgaag gagctgaccc ggatgatcac ttggtgggtg tccgtgtatc cgtcggccgg
    20461 gtcgccctcg accggaatgc cctgggcctt gagcgaggcg gcgaatgatt tggcattggc
    20521 ccggaccgcg gtctgaagct cctttttgaa ggcgttcatc tcataagaag ccatcaggag
    20581 acccagcagg gtggccaggt ggtgattcga agtcgagccg gggaaagccc ggccgcggat
    20641 gtcggtccag agatttttca gcggcgagga cggagcgatg tttccggcca cgagcccgcg
    20701 ctggggaccg aagaaggtct tgtgggtgct cccggtcacg atgtcggcgc cgtcctgaaa
    20761 cggctcctga aaggccccgt aaaggcccag gacgtgggcc atgtcgtaca tgatcaccgg
    20821 gcgcttcggc cagtccttga cgatcccggc ggtgaaggcg accggctcgg gatgaaggaa
    20881 catgctcttg ccgaagacga tcagctcggg cttcttgacc tcgagaagcg cggccagcgc
    20941 ctcaaggtcg atcttgtagg ggttgtcttt ccggacggga aagttgacca cgttctcctt
    21001 gcccgtagcc ggatcttcct cgatccaatt ataaagcgcg cccatcggct gggagctgag
    21061 atggcctccc ttgcccaggt cgttgttcat caccaagcgc atccggcggg agacttggcc
    21121 ctcggcccgg ccccggttca ggaacttcgt gacgcccttg aagacgatct cgttggccat
    21181 ctgcccgctg acgggccgga gctcgacgtc gacgcagccg aaaaaggcgg ctagctcccg
    21241 gcgggcttca atttcaacgt ccttgatgaa atcgatcccc tggtaaaaat agacttcctc
    21301 gcccttcatc gtccggtgct cggcgtatcg gcccgacgga tcggccatct cgcatatttt
    21361 cacgagcagc gagggcgtcg attccgaggg aatgaggttg aggcattccc gttggcgcca
    21421 gaggctgttg cgttcgatgc ggtccacgag ctttgcggtt ttttccgaca gcggcatagt
    21481 catcacgtcc tcctcaatca cattactcaa cggcggcctt tcagcaaccg caggatttct
    21541 tttcaatttc ggcgatcttg gccagccggc gttcgtgccg gcctccgtca aaaggcgtct
    21601 cgagccaaac ccggacgatt tccaaggcgg tctggttgtc caaaatccgt ccacccaggg
    21661 tcaggcagtt ggagttgttg tgccggcggg ccatttcggc cgtcgccggc gtccagcaga
    21721 cggtcgcccg gatgcccgga aacttgttgg ccgccatggc catgccgatc cccgttccgc
    21781 aaaccaggat cgcccggtcg caggttccgg ccaggacggc ctcggcggcc gcggcggcgt
    21841 aatcgacgta gtccaccgat tctcccgccc gcgacccgag atccgcgaaa tccacgcccc
    21901 gcccggtcaa aaaccgggcg atcatttcct tcatttcgaa accggcatgg tcggaagcca
    21961 gggcgatgtt cattggatca tcctcgccgc ctcgggcatg acctcgagcg cttgcagaat
    22021 caccgggtcg ttcttctcga acgcccgcca accctcctcg cccccccaaa gcagggaagc
    22081 gatttcccgc tcgagctcgc gcttgacctc gaccgcggct tccttgaatt ttgtttcgtc
    22141 ggccggcatc ttgttctcaa tcaaaaaagc ccggaaatcc tccagaacgg ccgcatcggc
    22201 aagaaagact cgactgagct gaatcatccc cggggccggc cgggcggcgt ttccatccgc
    22261 tggaaagaca aatttcttgg acaggggcgt ctggcgggac gtgaattttc gggcataagc
    22321 gaaaaacgcg ccgcggatgc ggagctcggc ggtatacggc agaaggttga agacgaccgt
    22381 gaggtccggg gtgatgcccc cctgccccag gaccttgcgg cccttgtccg tgtatttcac
    22441 ttcccggtcc gattcggcga ggaccttggt gtcgaggaaa taatcgtcca ggtgagagta
    22501 gtctcgctgg agggagcggc cgctcggcgt gtaatagcgg gcgatggtca aggccatggc
    22561 cgtatcctcg gagaggggga acatcttttg gaccaggctc ttgccccagg aatcctcgcc
    22621 caggataaga ccccggtcgt gatccatcac cgccccggcg acgatctccg aggcgctggc
    22681 gctcccttgg ctgatgagaa cgaccagggg aacggcttca tactggccgt tgcgggtcgc
    22741 cggatattga cggtcgaaat cgggattccg tccccggatc gagacgatcg actcgccccg
    22801 ggcgagaaat tcgtcggcga cgccgatcgc ctggaacaag ggtccgccga cgttgccccg
    22861 caggtcgagg accaggtttt tcatccccgc gtttctcaac gtctcaagct tcgcctccag
    22921 ctcggcggtc gtgttctcgg cgaaatagcg gataaaaacg taccccgtgg cctcgtcgag
    22981 cataaaggcg taggggaccg aatacagggg gatctcctcc cgggtgatcg tcagatcgaa
    23041 cggcttttcc agcccctcgc gggccaccgt gatcgcgacc ttggtcccct tggggccgcg
    23101 gagcttggag accgcgtcgt tggccgagat caagaccgtg ctttcgccct cgatatgggt
    23161 gatggtgtcg ccggcttgga ggcccatcct ccaggcgggc gtgccctcga tcggagcgat
    23221 cacgacgagc ttgtccatgt gcttctgaat ctggattccc aggccgaaat aggccccccg
    23281 ctgctcctcg gacatccggg ccaggttgtc cggatcaagc agatacgaat ggggatcgag
    23341 cgtctcgagc atcccccgga cggccgcccg ggccatcttg tccgcctccg ccgttcggaa
    23401 atattcggct tgaatcaggc cggccatggc cccgaccttc tcccgccctt ccacccaggg
    23461 atcccccgga cgaacggcgg cgccgaacag aatgccggcc cccaggagaa ggaataacgt
    23521 cttgtttttc atgaatttcc gacggcaaat cttatcacgc cgggcgagcc taagtaaagg
    23581 cgcggttctt gacttcccct gattcccggt ctatattttg aaggctttcc cgaaaatcag
    23641 aaacgcccat gttcggaaat atcggcttgc cggaactgat cgtcatcatg gggctcgcct
    23701 tgctcatttt cggcccccgc aagctcccgg aaatcggccg gaccatcggc aaggccgtcc
    23761 gggaattcaa aaaatccact gaagacatca aggaaaaatt cgaagagcag atccgcgtcg
    23821 aggaattcaa atccctccaa gccgacctca aaaaggacct gcgcgaagcc gatctcagga
    23881 aggatctttc ggggaatggg gaagactcga aaagctcctg acgagatgac cttcctcgag
    23941 catctcgaag acctccggac ccgtctcttc cgctcggtgc tttccctctt catcgccgtc
    24001 ttcccggctt acatcttcag caaggacatt ttccattttc tctcccgccc cctgacccgg
    24061 tttctgcccg agggcgaaca gctcgccttc cggacgctga ccgagccctt catgctctac
    24121 atcaaggtct cgttcctggc ggccttgttc gccgtctcgc cgtacatctt ctaccagctc
    24181 tggaaattcg tcgctcccgg cctctaccaa aaggagaaga aatacgtctt tcccttcgtc
    24241 ctcttcactt ccgtcttctt catcggcggg gcggcgttcg cttattacat cgcgtacccg
    24301 ttcgcctgcc ggttcttcct ccagctcggc tcggatttcc aagcggtgat cacggtcaac
    24361 gacttcttca acctgaccat caagatgctc gtcggcatcg gcctcgtctt cgagctgccg
    24421 atcctcatcc tgttcttatc gaaaatggga ttgatcacct cgcgatggat ggtccgcaag
    24481 ttcaagtacg cggtcctggt catcttcgtc atcgccgcga tcatcacccc gacgcccgac
    24541 atgatcagcc agagcatcct ggccgtcccg atgctggcgc tttacagcct gggcatcgcc
    24601 gtcgcctttt tcttcgggaa ggagcggaag gaccggaggg agcggcgggc gaagaaaaaa
    24661 gcagccgccg cggaagcctc atgagctccc tccggccccg gctcatcggc ctgaccggac
    24721 ccaacggggc gggcaaaggc gaggccgcgg cttttttcgc cgcccgcggc tatgcccacc
    24781 ggagcctctc cgacattatc cgggaagagc tggccgcggc cggccttccg gccgaccggg
    24841 accatctgat cgccaaggga aacgagctcc gccggaaagg cggccccgac atcctggccc
    24901 gccgcctgct taagctcgtg accgggccga cggtcatcga cagcatccgc aacccggccg
    24961 aggtcgagcg cctccggaag gaagacggat tcatcctcct ggccgtcgac gcccccgccc
    25021 ccctccggtt cgagcgcgcc cgccggcgcg gccgggacga atcggccgcc accctggagg
    25081 agttcctccg caaggaagcg gaggagcgga gctcgaaccc ggccgcccag cagatccacc
    25141 gttgttttga aatggccgac gccgtcgtga gcaacgacgg gacgctcgag gaattgcaca
    25201 gccggctgga ggctttccta tgaatacgcc ccgcatctcc aaggatgagt attacctcgg
    25261 gatcgcccgc gaagtcgccc ggcggagcac ctgctggcgc cgatccatcg gcgccctgat
    25321 cgtccgcgac gatcagatcg tctcgacggg atacgccggc gcgccccgaa aaacgcggga
    25381 cagcatcgag cacgggttct gccttcggga caagctccaa atcccccacg gccagcgcta
    25441 cgagctctgc cggagcgttc acgccgagca gaacgcgatc atcaacgccg cccgggcggg
    25501 cgtcagcctg cttcgcggcg acatgtatat tttcgggagc gtcccggacg gccggccggt
    25561 cgacgctttt ccttgtttca tctgcaaaaa aatgatcatc aacgccggcc tggtccgaat
    25621 cgtttgttcc acggccgccg gcggctccac ggcctttctc gtcgacgatt gggcccgagc
    25681 ctggcgggag ggagacatcc tcgatgacac gcatcaatac ggctgagcgg aaatcccctt
    25741 ggccgcggac agccgccttt ctcctggcgg ccgcccttct ggccccggcg gccggccccg
    25801 gcgaagagaa tcttttaaag ctcctgggca ttgccgtctc cgccgaatcc atcgactatg
    25861 tcgttcaaaa aacgcccttc cagcttcaca ccctcctgac cgagcagcgc catcccaaaa
    25921 cctggatgct cggccgccgc atcgccgccg atacggccga ggggctgcgg atgctgttcg
    25981 gagtggacga ggacatcgcc ctcaagctcg acggcctcgt ccgcgatccg gaacagctcg
    26041 gccgggtcga gcgccttacg gaggcgttcg ccgcggcgat gctcggccgg cggaagatct
    26101 acatgtacgg ctgcggggcc accggacggc tggccaagca agtcgagagc gccttctggc
    26161 ggccgttctg ggggggcctt aagaagaacg aaccgggcct ctgggcgaaa tgttccgccc
    26221 gtctcgatcc ggccgttgaa gacgaattga tcggcgagat gaccggggcc gaccgggctt
    26281 tgatcagctc gctcgaggga ttcgaggatc ttcagctcat cggccgcctc cagcttcacg
    26341 accgcgggat taagccgggg gacgtcgtcg tctgcgtcac agagggaggg gaaacgtcat
    26401 cggtgatcgg aacgatccgg gcggccctgg aggaccggaa ggcccgtccc ggatatgatc
    26461 ccgccgaatc gcgaaaagct ctgttcttcg tttacaacaa cccggacgac cttctccggc
    26521 cgttcgagcg aagccggatc gtcttggagg atccgggcat caccaagatc aacctgacga
    26581 ccggccccca ggcgatcacc gggtcgacgc ggatgcaggc gacgacgatc gagacgttcg
    26641 tcgtcggaag cgccctggag acggccgtcg agcgggtgtt gcggacgatg ctgtcgaaaa
    26701 aggaaatggg ccggttgggc ttctcccggg aaacaagcct cgaggatcgg ctcaaggcct
    26761 tcgccccgct tcttccggaa atcaagcgca aggccggaga gatcgcccgc ttgacggacc
    26821 tcgaagcccg ggcttacgcc ggcgggcgca tgtcgacgta tttcgcccgg gagggtttga
    26881 tcaccgtctt catcgacagc accgagcgca gcccgacctt ccggctggcc ccgctggaca
    26941 cggtccgcga accgcggagg aaaagctgga tccaggtctg gacggcggcc gaggctcgcg
    27001 ctcaagcctg ggaggctttt ctcggacggc cctttcgcgg gctcgatccg ctgttctacc
    27061 gcaaaccgtt cgaaaacgaa atcgacgacg tctacctcag ggacaaggcc cttcaaagcc
    27121 tcgcccgggc gggagacgat caggccgccc tctacgattt ttccttcgga ggaacaaacg
    27181 tccaaaagcg agccccccaa aaaggggacg tggcgatcct ggctctcgtc ggggacgaga
    27241 tcggggaaag ccggaacccg cgatccacgt ttttccggtt cgccgagctc gcggcggaaa
    27301 agggcgcggc cctgggcgtc ctttccatct gcgatctaaa taaggagaag aaagcggccg
    27361 gaggcgtttt tccaagtccc cggatcgacg tcgaattcgc cgtgtcccgg gaaaacgatc
    27421 cgatggggat ccgtcagcaa atcgccttga agatgatcct caacgcccac tctacggccg
    27481 tcatggccaa gcttggaaag gtcgtgggaa acacgatgac caacgtcagc ccctcgaatc
    27541 tcaagctgat cgggcgggcg acgtatctcg ttcagcttca cgccaacgac atcctgggca
    27601 aaccggaatg ggtgaaggcg ttcggcgccc ggcccccggt ctcctacgcc gaggccaacg
    27661 ccgtgctttt cgattccatc gccttcctga aggaccggcc ccagggcgcg gccatctctc
    27721 ccgaagtcgc cctctccatc gtccgcatcc tggaatcgct ccggcggaaa atcgggataa
    27781 cggccgagga agccctggcg atcgtccgga aaacgagcct gcaggattac ttggaccaag
    27841 ccccccggcc gccggcgccg ggggaatgac cggagtcttc gaccgccgcg gctcacccga
    27901 tcggcgggag gccggtcaaa tcatagcagg ggctgatcga cggccgccgc tccttgcggg
    27961 gggctttttt ctcgggagcg ggccgatcgg atttcggctg cgggtaaacg aacgtctccc
    28021 cctcgaaatt ccgcatgacc acctgttcgt cggagatcga ttggatgtat ttcgggagaa
    28081 gcgggatctt tccgccgcct ccgggcgcat cgatgcagta atacggaacg gccaggcccg
    28141 aggtccagcc gcggagggct tccatgacct cgatcccctt ctggaccgtc gtccggagat
    28201 gatccgtccc gctgacatag tcggcctgat agatgtagta aggccggacc cgggccatga
    28261 ggagcttctg catcagccgc cgcatgacca gcgggtcgtc gttgactccc ttcaagagaa
    28321 ccgtctggca gccgagcggg atcccggcgt cggccaggag gccgagggcc tggttggcct
    28381 cgggggtcag ctcgtccggg tggttgaagt ggacattgac gaagagcggg tgaaacttgc
    28441 ggagcatcgc cgccagtttc ggagtcaccc gctgggggag gaagcaggga acgcgcgttc
    28501 cgatccggag gatttccacg tgaggaatgg cccgcagcct ggtcaggatg tattccaggc
    28561 gggcgtcgct gagcatcagc gggtcgccgc cggaaacgat cacgtcccgg atttcctcgt
    28621 gggttcggat atactcgaga ccttcctcga tggccttcgg atggatcttc ttcggatcgc
    28681 cgaccttgcg cttgcgcgtg cagaaccggc agtaggaggc gcaccgatgg gaaaccagga
    28741 acaacaggcg gtcgggatac cggtggacga cgctggggac cggggaatcg tggtcctcgg
    28801 ccaggggatc ggaaaccccg ctgtgctcga ccagctcggc cgggtccgga acgacctgcc
    28861 ggtagatcgg atcgcctttt gttttgatca agccggcata gtacggagtg atcaacgttt
    28921 tgaaatgtcg ggaaatccgg cggacttcgg ccacgtccaa gtcaaaggcg caggccactt
    28981 ggtccggcgt ccggaggctc ccccttgcga tttgagacca atcgtccatg gtcctttctc
    29041 ctctagctca ctcgcttcgt atagatcacg cgcccgtccc cgggtccgta aaaatcaggg
    29101 atccgggcgg tttcggtgta attcaacccc aggtaaaaac gcctcgtcgg ctcgtatttt
    29161 tcgctggagg acgtttcgat tacgatcagc cgcccgccgg cccggcggac gtggtcctcg
    29221 agccattcga cgagaatccg gccgtatccg cggccctggg ccttgggatc gacggccatc
    29281 cagtacaggt cgtacgtccc ccgggtgagc ggggtggggc cgtaggtcat atatccggcg
    29341 accgcgcccg tctcgtccgt cacgacgacg atggcatagt cctcctgctc cggccggccg
    29401 agagtgatgt cgatcagctc ctcggcgacc gcgatttcgt ccggtcggaa aaatcccgtc
    29461 gcctcgatca gggccaggac ggccgggcgg tcggcggggg tcatgggccg gatcatcgct
    29521 tttctccccg aagcagagct ttatcgatca tctgcttcca aaaattctta tattcgacgc
    29581 cggccgcgcc caaagcccgg gcgtacccgg cgttcaagct gatgtcagga ttgggattga
    29641 cctccaggat gaacggccgt ccttttttat ccacccggta atccacccgg gcataatccc
    29701 ggcactcgca ggcggaaaag gccttaaccg cgatttcctg gaggagcgtc cggagcccgt
    29761 cgtcgatcga ggccgggcag cgcggcggcg tcgactgaaa caggacgtcg tccggatgcc
    29821 acttggccgc gtaactgcag atattcggct gattctcggg catgccttca aactcgatct
    29881 cgctgaccgg cagggcctcg gtcttctccg cgtccctcac cgcgacgttg aactcccgcc
    29941 cttcgatata cgcctcgacg agggccggtt ccccgaagac gtcctggata tgcccaagct
    30001 gccgacggag gccgtcctca tcccgaacga cggattccgg gtggatgccg aggctggcgt
    30061 cctcgttgtt gggcttgacg atcaggggga aaggaaggtt gattttctga tccggggatt
    30121 cggccagcac ggccggcggc gttgggagcc cgaacgattt cagaaccgcc ttggttttgt
    30181 atttatcgag acacagggcc agagtccgag aatcgttccc ggtgaaggga acgcccagga
    30241 gctcgagggc cgcggccacg ttcgcttcca gctgcggccg gcccaggaac gcctcgcaga
    30301 gattgatgag gacgtcggcg ttgagggtct tcagccgctg gcagaagttc atgaaacttt
    30361 tctgcagggg aatgatgaac gcggcgtagc ccagctcggt gacggccgtc aagacttcct
    30421 tggccgtttg ctcgacggat tcctccgacg gccggtcgac cttgttcgcc gtgagcgggt
    30481 cataggcgtt gaaggcgatg cccacgagaa ggttcgggcc ggacttggcg ctcatgaccg
    30541 gcctttccgg gaagaaacca aaccccagcg gcggcgggct tcgtccacga cccggagaat
    30601 gagctcgtcg tacgaatacc cggccgtccg ggcggctttg ggcaggcagg aattatcctc
    30661 gggattcgga aggattcccg gaaggggatt gacttcgaga atatgagggc gccccttctc
    30721 gtccaggcgg acatcgaccc gggcccaatc gcggatccgg aggatccggc aggtgtcggc
    30781 gacgatcgtc cggatctcct tctccaagcc ggaagaaatc cgggcgggac acttgaaaat
    30841 cgccaggggc ttttcgggag tgtcccagat ccatttcgct tcgtaggaat agatcggatg
    30901 ggcttcgggg ggaagaaggg aatgatcgat ctcgacgatc ggcaggatct cgaggttggg
    30961 gacgttgccc aacacgccca cggtgaactc ccggccggtg agaaaccgct cgatgatgac
    31021 gggctggcgg taaaggccgg tcacttcccg gacgcgggcc gccagttcct ccgccgtggc
    31081 gacataagaa ttgtttttga tccccttgct cgatccctcg tagagaggct tgacgatcgc
    31141 cggcagcttg atcccgtccg gaacggcctc gccgggctcg acgacccgga attcgggatt
    31201 cggcacgccg taatacgaga ggatttcctt ggcccgcgat ttgtccaggc acaggctcag
    31261 cgtcagcgga tccgatcccg tgtagggcag gtccagcatt tcgcacagcg tcggaatgtg
    31321 gccttcccgg ttggggccgg tcaaccgctc ggcgatgttg aagacaagat ccggccgggt
    31381 gttccgaagc cgcgcgtagg cgtcttcgtc ggactcgatc gggatcactt catatcgccg
    31441 gcccagggcg gcggccacgg cggaaatggt ttcgtccgag tcgcactccg caaacgtatc
    31501 cgacggtgga ggctcctccc cctcgtcctc gacgaccggg agaaccgccg tcgcttcggc
    31561 gacgctttga cgcagagagg cggccattcc tttttttgaa ctgaagagca aagccacttt
    31621 catcttcgct cttcggtccc tcgcctcaag ttgaaataga tcccctgctt cgtaatcatc
    31681 tgctgccgct tttcaattca tcgttcttca aaagtagtta tagaacattt aagggaaatg
    31741 tcaatagggt ttgtcaagga aaatacaaat tattcgttta gaccccgcgc cgccccccat
    31801 tagtagtgct gtctagaagc cggacatgtc agcgcatgaa ctcgctgtaa agatagtaag
    31861 cccggggatc gagcacgcgg cgggacgcct tcgcccggag cgcccggtag ggggaccggt
    31921 aatcgctcgg cggaacatgg aaaaggagaa aagctccggc cttgtggaga tcctcgaggt
    31981 cgggctccgg aatcaatctt tccagatgaa tcaccaatcc cgccccggcc gcggcggcct
    32041 tcgccgcaat gtcgcgcagc aggagcagag ccggaagatc cgcgccggct ccggcggccg
    32101 acacatgaat ccaggcccct cgccgggcca gctcttccaa agcctcggcc ggagccccat
    32161 gatggtagta tcccagccgc cggtttcccc tccgcgcgtc ctcggccaga acggccaagt
    32221 cctccgtccg ccgtttttcc gttttattcg aaagataaag agaaacgccc ccgcccgccg
    32281 cgtcgcggag agccctgagg gagaggacga cgccctctcc ttccgcaaga agagggccat
    32341 gctgaaaatg agcgatctct ccgccgcttt tcccggcggc ggcgctcagg acggcgaggt
    32401 cggccggcgc gcggccggcc ttatcggagg tgaaaagatc cgcgcccgaa agacagatcc
    32461 gctccaattc aaggacggtg aggcgcccgg catcaatcag atggaggcgc ccgccccgcc
    32521 ggcgccgtcc ccgggcttcg tccaggattg cataaaaatc cgcctggtca tcccggagga
    32581 caagggtccg cttcttcgat gggaaatcgc gctcgtccat gatcgatccg ccggatgcga
    32641 agtgtacctt cgcccccgga ccgtgtcaag aaaacgaatc gcgccgcctt aaatcacaaa
    32701 aatcttgccc cggcgcatcg tcccttaccg tttttaatct ttagacgatc ctcggtttcg
    32761 gtaaggtttt gatatggcaa gagaataccc tcttgggcaa ggcttttata ggcttgaccg
    32821 gaaaactcgg aaccggccct tcgatttcag cggccgccct cccctcgagg acgatgagtt
    32881 cccgtcgctc gtttgaaccc gcttccttat tcttatatag taatcgcggg gcaggcggat
    32941 gcgcaaaaaa accctggttc ttttctccgg agggctggat tccacgaccg ccctccattg
    33001 ggccctccgc cggggcggca aggtctcggc cctgaccttc gactacgggc agcgccaccg
    33061 ggtggaagtc cgggccgccc ggacgatcgc ccggcggctg ggcgtccccc acaagatcct
    33121 gaaaatcgac ctggagcaga tcggcggttc cgccctgacc gagccatcca tcgccgttcc
    33181 gcgccacaag cggctcccgc gcctccggac gaccgcaccg ccggtcacgt acgtcccctt
    33241 tcgcaacggg atcttcctgg ccttggccgc ggcttgggcc gaagcgcaag gaatcaacga
    33301 gatcgtttgc ggcttcaacg tcatcgattc gccggcctac cccgacaccc gccccgtttt
    33361 cgtccgggcc atggaacggg ccgtccgagc ggggacagcg gcggccgccg ccggccggcc
    33421 ggtccggatc cgggcgccgt ttgccgggat gaagaagtcg gagatcatcc ggacgggctt
    33481 ggccttgggg gcggattatt ccctgtcgat cacctgctat tcagggaagg aacggccgtg
    33541 cggctcgtgt tccgcctgcc tcctgagagc tcgggctttc aaggaagcag gctgttccga
    33601 tccgctcgtc ctgcggacag gcaaaggaga acttccatga gctggacgtt gaaagtccgg
    33661 gaccgtttct cggccgccca ctttctgcgc gaataccagg gcaaatgcga aaaaatccac
    33721 gggcatactt ttcaagtgga agtcgaggtc gacgtttccg agctcgacgc cgccggcctg
    33781 gggatcgact tcgccgtctt gaaggccgga ttggccgaaa tcctacctga ccacaccctc
    33841 ctcaacgacg tctatccctt caatccctcg gcggaaaacc tggcccgtcg tttctatgaa
    33901 gacctcaagc ctcgttttcc ggtccgggcg gtgacggtct gggagtccga ggatgcccgg
    33961 gcgacctacg ctgaagatcg ctgagatctt cgccggaatc cagggcgaag ggctccggat
    34021 gggaaggccg gcggtgttcg tccggctggc cggatgcaac ctccgctgct cgttttgcga
    34081 cacgaaaagc gcttggagag gcgggcggct catgacggcc gcggccgtcg ccgccgccgt
    34141 gaaaaaagcg cgaagaccgt ggtccggcga gtggatctgc ctgaccggag gggagccgtt
    34201 cacccaggac ttcggaccct tggccgaacg cctgcgggcc gagggattcc gcatccaggt
    34261 cgagacaaac gggacgatct tcaaagatat ccctctcgat tggttcacgg tctcccccaa
    34321 gccgccggat tacgatgccg cccccgaatt ccacgcccgg gcccgcgaag tcaaacttgt
    34381 cgtctcccgg gaactgacgc ttcgcgcggt cggccgcgtc cgggccgcct ttcccgcctc
    34441 cgttccggtt tttctccagc ccgagtcgaa ccgtccggaa agccgcgacc gggcgatccg
    34501 cctcctccgg cgggcatcgg cccgagggct cggggacgtc cggttgggcg ttcagctcca
    34561 ccgcgttttc ggtctgcgct gagccgcggg tccatccgcc ggccgcccgg cgttttcggg
    34621 cggatcggga gcggccgccg ccgcaacgct tcccctcgag accggggcgg gccggatccc
    34681 gtttcgcccc ccgagaaaga agccgtactc cgtccgggcg agaaaaacac cgaggagaat
    34741 gaccgccgcc ccgccgatct ggcgcggccg gagcgcttcc cccaggatga gcagggcgaa
    34801 caaggccgtg aagaccggcg tgagattgct gtaaacggcc gtccggatgt ttccgacccg
    34861 ccggaccgaa acgtaccaaa ccaagtagcc gacgaccaag ctgaagacgg ccgagaaccc
    34921 gaggccggcc caggcggccg gcgaaacgct ccgccaagaa atccgcgaaa tcgcgccggc
    34981 acagaacggg aagtaggcca gggcgccgag gccgacggtg atcgccgtga acttcaaggg
    35041 cgacaggcgc tcgaggaact cctttgaaaa aaccgtgttc aaggaccaaa gaatcgttcc
    35101 ggcgaaaatc atcacgtcgc cctggaagcc cgcgctcaat agccggattc ccccttcccc
    35161 ccccgcgatc acaagataga ggccggccgc ggagatgacg atgcccaagg ccgcccccca
    35221 gcggactttt tcgattcgga ggaggccgcc gatcaaggcc acgatcgccg gcgaaaacga
    35281 cagaatgaac gaggagttgg aggccgtcgt ccggctcagc ccgccgatga acaggatctg
    35341 gtaaagggcg tggctcatga tcccgagcgc ggccagcttc caaaactcgc ttcggccgac
    35401 gcggaagctc tcgccggagg cggccatcaa gccgacgagg acggcggccg tcaggaggag
    35461 gcgcaagccg ttgaatccgg ccggcgaaag ctcccggagg gagatcttga tgatggaaaa
    35521 attcagcccc cagatcaggg tgaccaggag catgaacacg tcgctccagc cgaacgacgg
    35581 attcttcggg ggaagggcgt tcatgggcat ccgggaagtc ggcgggaggc gttcaaacgg
    35641 ccgcgagtcc cgccgtcagg gacgtttctt cgccctcccg gcgtcttttt gagcccgcgg
    35701 ataggccgct tcgatgacgg agaggaggcc gcccctgggg ttgaaggagc cctttaccgc
    35761 ggcccggacc ggacgggcgg ccgcgacgat gtcctccagg atccggttga cggcgttctc
    35821 gtagaaaatg ccgagatcgc gatacgccag gaaatattct ttcaacgatt tgagctccag
    35881 gcacgagtcc cgggggacat aggtgatcgt gatcgtcccg aaatccggca gccccgtctt
    35941 gggacagacc gaggtgaatt ccggaaccat gatcgtgatt tcatagtccc ggaattgatt
    36001 gggaaaaacg tcgatggacg gaagccgggt tttgaggccg gccgtcgctt cctttttacc
    36061 gtatccgctc atctggaacc gtcccggaga acgtgttagg atatgatatc cgatatcccc
    36121 cgggatgaca atcgacatgg cttcaccgtc ttttctctac ggcccggttc cgtcccgccg
    36181 gctcgggtat tccctcggcg tggatctttt gccgtttaaa acgtgctcga tggactgcgt
    36241 ctattgtcag ctcggcggag gggcccgaac gaccgtccgg cgccgggctt tcattccagt
    36301 cgaaaccgtc ctggccggga tcgagaacgt tctccggcgc ggaggccggg tcgacgccat
    36361 cacgttttcc ggatcgggcg agccgaccct ccatccgggg ctgggacgcc tcatccgggc
    36421 catcaagcgg aagaccgcgg ttccggtggt cctcctgacc aacggctccc tgttcacccg
    36481 cccttcggtc cgaaaggacg cggccgccgc cgacatcgtc gtcccctcgc tcgacgcggc
    36541 gacggaagac gccttccgga aaatcaaccg cccccatccg ggtctgtcgg cggccgcgat
    36601 catcgaaggc ctggccgcgt tccggcggca tttccacggc cgcctctggc tggaggttct
    36661 tttcgtcaaa gggatcaacg accgtccgtc cgatctccgg gcgctcaaaa aagccatcgc
    36721 ccggatccgg cccgaccgcg tccatctcaa cacgatcgtc cggcctccgg ccgagtcttg
    36781 ggcccggccc ctcggccgcc gggatctcga aaaaatccgc gccttcctcg gagaaacggc
    36841 ggaaatcatc gccgatttca aaaaaccggg gcggcggacc cgaacgaagg atctcccttc
    36901 ccggattctt gatttcgtcc ggcgacggcc cgagacggcc gaaaggatcg ccgcctccct
    36961 gggcgcttcg ccggaagccg tcgagcgcgc cggccggcga ctcgagaaag acggccgcct
    37021 ccggctcgtc cgcctcaacg gccgcgtttt tttcgagccg ggagccgccg ccgggaaaaa
    37081 atagggccgg ccgcctccgt cgctgatgga tcggcccttt aaatccctcc tcccgatagg
    37141 ggaaaaggat ccgcttggct tgacgacgac aaaccctcat gatcggagcc gtcgccttaa
    37201 gcttcgggct cccctttagg atctgttcaa acccttccat ccgctggact tcgggccggc
    37261 tcaagatgat tgtctcctcc atgtccgcct tcatcccgaa gcggacattt ttgctttgcc
    37321 cttaaagggg acattttcgc tcggcagtta gggggacata ttgactcggc ttaaacag
//
LOCUS       DAOOXH010000015        36064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_981_length_36064_cov_7.865450, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000015 DAOOXH010000000
VERSION     DAOOXH010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 36064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36064
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_981_length_36064_cov_7.865450"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="PKV64_03345"
     CDS             complement(<1..107)
                     /locus_tag="PKV64_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPN16149.1"
                     /translation="MKISAVVITYNEEANIEAALRSLEGVVSEIVVVDS"
     gene            217..1656
                     /locus_tag="PKV64_03350"
     CDS             217..1656
                     /locus_tag="PKV64_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPN16150.1"
                     /translation="MTAYERLLAKAFDPEEFRREGKKIVDLLADFLAASEPEGRNRVF
                     AWEDPESMFASWAGRFAAKPSRPFADLLPEILDRSNNLLHPRYVGHQCTTALPLAALG
                     GLAGQLLNNGTAIYEMGPVNAAMERHLIRWMAGLAGFGPGADGVFTSGGSGGNLTALL
                     AARQAAAEGDIWRDGAAGAAPLAILASSQCHYSVRRSAAIMGLGEEAVVPVDVDEKFH
                     MTASGFKTALAEAGKRGRRPFALVANACSTATGSYDDLEQAADWAASAGLWLHVDGAH
                     GAGALLSPKYRSLLRGLERADSFIWDAHKSLLMPALVTAVVFRDGGRSFEAFSQNASY
                     LFEKTATEEWYNFAHRTLECTKTMMGMRLFISLAAYGTDFFAGYVTAAWDLAREFARR
                     LRTAGDFETASEPESNIVCFRHLKKGEPDLDGLQRRIRRILLEQGRFYVVQTQLDGRT
                     WLRCTLINPRTTAGDLDALMEEIRSAGRS"
     gene            1781..2551
                     /locus_tag="PKV64_03355"
     CDS             1781..2551
                     /locus_tag="PKV64_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018283269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPN16151.1"
                     /translation="MAGSDESVFLSVVVPAYNEDARLGPTLRTIRAHLESRINAAGTG
                     NGPTEIIVVDDGSTDRTGAVAAEALRGRPRDRVLRRGKNRGKGFSVKEGMLAAAGRLI
                     LFTDADLSTPITELEKLLAGIRDGHDIVIGSRALPGAEIRRRQRVLRERMGKTFNLFV
                     RGWVLKGFPDTQCGFKLFRREAARDIFSRVRLDGFSFDVEVLFLARRLGYSIGQVPVV
                     WINSPESKVRIVASSVSMLRDLFRIRRLHRNAGFRRSS"
     gene            complement(2593..3897)
                     /locus_tag="PKV64_03360"
     CDS             complement(2593..3897)
                     /locus_tag="PKV64_03360"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPN16152.1"
                     /translation="MRFDELSLDPRLLRAIADRGYTETTDVQTRTLAETLAGRDAAVQ
                     SQTGTGKTAAFLVTIFDRLLRDGSRKSGKTLIITPTRELAVQIEGETDLLNRRLGFTI
                     GCFYGGVGYEQQEAVLRKGADILIGTPGRLLDLEEKGTLRLGDVRILVIDEADRLFDM
                     GFLPDIKRIVRKLPPAGRRQSLFFSATLNRLARRTAVEFLNDPVFIELTPERITVEAI
                     SQELYRVGSHLKANLLLGLIKAEAPKNALIFTNTRHAAERLAKKLEKNGYRGRFLTGD
                     LPQNKRLRIIDDFSAGRFPFLVATDVAARGLHIEGLEMVVNYDLPEDIESYVHRIGRT
                     GRAGKEGKAVSLACEKYGDKLSEIEAYIGMRIPIKTATPDLFVSDRGFEAELRAREER
                     KRSRGFGRGRGFAGDRRSARQDDFGSRPGPSGPELRPSPAVK"
     gene            4101..4880
                     /locus_tag="PKV64_03365"
     CDS             4101..4880
                     /locus_tag="PKV64_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16153.1"
                     /translation="MPPGKSFPLLLGAALTAAFLAASPPAAASVEIRIGGGGWTLAPF
                     HPLIESRSKEAVQAEFNAFLEAALPQGMLSPVRADIDLASSGGSLFAELWLPLGRGRF
                     ALGVRGDVFRFRIPFTAAASETIQLIGFPLAELNGTAAGTVRLEGLGLSLLGRWTVVS
                     AGSFEVGLRAGISVLPFRGRLSADGILQARTILGNFTYAGGLEGTMAEIREGGADVPS
                     LVLAPAAGLETAFRLDRRWRIFAALTVSHGTFYSAGLSGSF"
     gene            complement(4937..5308)
                     /locus_tag="PKV64_03370"
     CDS             complement(4937..5308)
                     /locus_tag="PKV64_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN16154.1"
                     /translation="MKREPAVLLAEDEALIAMDLSARLKNAGCGRVQVVATGEEALAW
                     AGRERFDVIIMDNHLAGSMDGVETAARLRAEGAVPFVFITGYPKEEICGGRTLSLAPF
                     ICLEKPVDFNQLLAAIEALLS"
     gene            complement(5305..6882)
                     /locus_tag="PKV64_03375"
     CDS             complement(5305..6882)
                     /locus_tag="PKV64_03375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HPN16155.1"
                     /translation="MKKGNRGGAPSLKERKSASLKPLRADRRSERKTVPLRNAGASAP
                     DGECGKKFSVLFKSLTQGVVYRDARDAVIDANPAALKMLGCARKELLSPDPSRPGWIL
                     TGEDGLPLRPADHPSKVALRTGRPVRGVRVRVIQSRSGNVRRLIVDAVPEFRGGGKTP
                     FRVLVILQDVTERKRLEIKLREDEEKYRTLFGTSDEAILLMIDEMFIDCNDRSVRLYG
                     CSSKEDLIGHSPWEFSPPAQPDGRDSREKARAYIAAAVAGAPQRFYWRHRRKDGGLIE
                     TEVCLNGLVLNNRIHVQAIVQDIGERKRAEEEIQRSLREKETLLREIYHRTKNNMNVI
                     GAMLSLKSRAIDDERIAAVFRDIEDKIRAMALVHRKLYESKDLHRIDLKDYVSDLAAQ
                     LAESHPEAAAAVEVKLDLEELPVPIDVAIPCGMILNELFSNVYKHAFPGSGRGEIRIR
                     LARSTDGKTIELDFADNGRGVPPGFDLLAQATLGLQTLVMLVEHQLRGAVRFETEKGV
                     TCLIRFPSKAFFAEEGA"
     gene            complement(6914..8455)
                     /locus_tag="PKV64_03380"
     CDS             complement(6914..8455)
                     /locus_tag="PKV64_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPN16156.1"
                     /translation="MNIRRIILALLTIGAAFGPLAAEDGAVWPLTLDEAIARALKNNL
                     DIAVESYNPELAEGQLFKARETFLPQFDLSFGDDHRESPSYWWLQGEGTSIDKYRNYS
                     AGIAQMIPTGGRFSLSFQGYRSETNQSFQLINPRFGTTLAVSFSQPLLKDFGLKIARR
                     QILEAENSLEIADVQLRGAMMETIYLVQEAYWNFVYAVDSLQVKRQSLELGRDLLAKN
                     KKEVEFGQLAPIEILNAEAAVAQREADLIQAEAMVTRSREVLKTILNLPAEAPGTILE
                     PVDRPVAQGVSLSLDEALKQAAANRTDLQALRKTIDNKSLSLEFARNQMLPALNFEFS
                     YMSPGISGDRIKYLDDNPMTGIIIGKEPGSAGDATKDAARFRYSNWSVGLTLSFPLGH
                     ALTRADAAVARIDLAQSEARLKSLERQVALDVADAVRAVETDAKRIEAYRLARELAEK
                     SLEAEQKKLAVGMSTNYFVLDFQDRLANARSLELKAKIDYILSVERLEKATGMSLVRR
                     GVRLP"
     gene            complement(8564..10150)
                     /locus_tag="PKV64_03385"
     CDS             complement(8564..10150)
                     /locus_tag="PKV64_03385"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPN16157.1"
                     /translation="MNDRAASIPDPPGTIFLELTSACNMHCAFCPSDVLRRPKEHLSE
                     ARLKSFLGQLHDLGLRPPVLLNILGEPLLNKKVYPLLDDLEAAGHPVTLITNMTLLGD
                     GALRRELLRHGNLTLALSFQTATPRSYALRGYSRLPFRAYERILWKVIEDKFRLNSGA
                     RLEMHVASNYVMSRDATIQADGGAGLWANFPDEKAEGRWIARTLRRLERLAKRMKRRY
                     PDSFASARETAAVKYRDHVGTKIAVDRAGLPPGFHRLKDEVFWGYMALPDVFLVFKSL
                     ELWTRDRTFLQTAFPGRFTYVEERLEPRPCPMTGNLGLLAGGNFVLCCLDYEGEMDLG
                     HIDRTPVADVLRAERRAAVRRDAMAEALCRRCRGNLFVFETTLLPDRAEQTVDKFGRG
                     FWPREDGLHGVGGRWTDGKGWAYVYARLPARRIRLAFRSDFDDDAPLRLAVSAYDRDA
                     GAFPAVSASWPLAGRKGRTAVFEAGFDFLSDRLYRIEIESPSFVPDETLGNSDTRRLG
                     LAVYSISIGTTPAPPPEPGR"
     gene            10237..12621
                     /locus_tag="PKV64_03390"
     CDS             10237..12621
                     /locus_tag="PKV64_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16158.1"
                     /translation="MKILRRILRPIVFFVLSAGIALILRLNVQAPDFTIRNHAFRPEA
                     GPLLVSAILIFILLAGLWALISQIWTKNSRLSYKEALALDFPTYLPALFFLLAPLALV
                     HYLTRDDLRERLGLLAAGVLLAVLYLKAVRVAQADRQGKSNWGAALDRFLAWPTRRRL
                     ILLSVLALLLINGGALLVSGRGVSFGGDEPHYLLITHSLLKDGDLDLANNYKDRDFEA
                     YMPEGVAMRSHTVPGKKPESRYSFHSPGISVLLLPFYALGLALGKSALVFLVRFALSL
                     VGVFFGIQVYLYARQAWARERLAFALWALVTLSAPVFFYAFHIYPELVVAAAGMYVFR
                     RLRFKPARRAGDCVLIGILIVSFVWFHALKYAFIQAPLFLYAILRIWKGNDPADRGRR
                     LAALILPAGVFAAAYFAVQHALYGSLNPTSVSWQGAMDGKQTLSFLKELFTGIPFRFR
                     WETLAGYFLDQRDGLLLYSPLYLFAFLGCVSLFRKKAGDAGWLLLIALPYVLASAFLT
                     QRSGYAPQARPLVAVIWVPAIFLGAFIAEGRKHAFRYLFNGAAGLTLLMTWLLCRHPY
                     ALYQETTMGVTERAGSLFITLSNLHVYLPRLLPSFIKVKETGWTPNAVWIGALVLFVV
                     LFALRRPSDKRLPFPGHAAAALVLLALFFVQYVYYPRPVLMSPRPIVHASGEPWTFYS
                     LSRVAIMNEPGRFDILQDNRDYDFYFTSNRPLEKLGVEFGSPHGDYALRLNIADAAIF
                     SVTTRREVMTRTVESPPAYPWKGRFLYRVSIRLERKSDVRTAVTPYLFALSPAR"
     gene            complement(12643..13419)
                     /locus_tag="PKV64_03395"
     CDS             complement(12643..13419)
                     /locus_tag="PKV64_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPN16159.1"
                     /translation="MKTAKRGKRVVVVMPAYNAEKTLIRTLDDIDRSWVDDIILVDDG
                     SRDGTVDLARRLGLRVFVHEKNTGYGGNQKTCYTEAMKLGGEIMVMIHPDHQYDPRVI
                     PQLVQPLLDDACDAVFGSRMLGGRPLEGGMPKWKYLSNIFLTAVENIAFYMYLTEYHS
                     GLRAYSRRYLEAVDFRANSDGFVFDSEIIAQGVLAGMRIREIPIETRYFPEASQVGPW
                     GSIHYGLSILRMLVKFKLHKKGWIRYPMFELPGPAPKPGA"
     gene            complement(13518..15503)
                     /gene="ftsH"
                     /locus_tag="PKV64_03400"
     CDS             complement(13518..15503)
                     /gene="ftsH"
                     /locus_tag="PKV64_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPN16160.1"
                     /translation="MAANDEKKEILQARNGKNKEDPGLKKNDPLFKPLDRKQFKFSIW
                     YFLFTLVALFLINTVLLRPKYESIDYSDFKARIKSGEIVRVEISEKYVTGFTRKPSPE
                     TAPGKPEPGEPAPAAASITYRAVLMADADLVSLMDEKGVDYYAVAPENRSWLSALLSW
                     VFPIVLMVFFWNYLSKRIGGAGQGVMSFGANKAKLVAEGDTGVTFKDVAGVDEAKAEL
                     VEVVDFLKNPRKYEAIGGRIPKGVLLVGPPGTGKTLLAKAVAGESQVPFFRISGAEFV
                     EMFVGVGAARVRDLFKQAREKAPCLVFIDELDAIGKSRGITLGGNDEREQTLNQLLVE
                     MDGFDSRSGVIILSATNRPEILDPALLRPGRFDRQVLVDRPDLKGRVDILKVHIRNVK
                     LDPSVDLDKIARGTPGFTGADLANIVNEAALLAVRAGRASVLQRDFDEAIERIVAGLE
                     KKNRLLNPKERSIVAHHETGHALVAAYAKGADPVHKVSIIPRGIGALGYTIQLPTEDR
                     FLLTEDELLGKIDVLLGGRASEQVFFGEISTGASNDIARATDIARKMISDYGMSEKFT
                     NVVLPSQRSPIFLPGEPIAGQREYSEFTQQYIDEEITRIIKQRYDKVVAFIEEKREVA
                     ARVAARLLEVETIGAEEFKSLIGAPAGPPEVRPDGGG"
     gene            complement(15636..16532)
                     /locus_tag="PKV64_03405"
     CDS             complement(15636..16532)
                     /locus_tag="PKV64_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16161.1"
                     /translation="MRKMTAAAVAAVIALAASAVAEDLKESLAPKLQRIIGLQAEVGP
                     LHPVLQNLYPVAVVDGDRFLIFDLDESKTGYRFLKEAPVPMPMPKGVRAAFPLEALDN
                     RSACVVSPEVFDSARGYVTIFHEFVHCRQWETVEPALKGRLSIYRQAMEAGDYMWELS
                     YPFPYENAEFAEDYPAALDRMDRGDGPEALRIRARLKDLLGPENYEYLCWQEWKEGFA
                     RYLENAILRHLNMEENHGGLALPLTRVAFYEGGSRFIAFLEGRENGLTLNLETLFERI
                     ADPSGEPAGNSIRPTGPSPTTH"
     gene            16822..19248
                     /locus_tag="PKV64_03410"
     CDS             16822..19248
                     /locus_tag="PKV64_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16162.1"
                     /translation="MDSFVFLTPPDKSIITAFMTAVPESAVSRPVLWASAAGLAAYGT
                     AWFRAAGATVFDHGFRPVGITAALFLGLAAAALILLSRAARSWQDEAFGFGGPLWPPA
                     VLLAAPFLTIDYFDRDDLQRRLLLLGLLAAAAIVYLGVAGPASRLESSRPRATRLLDG
                     FLRLPVKRRLLVLFLTAFLVYNACALVLVSRGMTFSGDEPNYLLTTHSLLADRDINLE
                     NNYRNRDYFHFYDEKENPNLRMNPYARAGVKGRGYVYPINLPGISFLMVPFYAAGQAV
                     HGFARTFLLKGSLAVWAALLGLQIYLLARKLWKREGLALGLWALVSFTPPVLFYAVHL
                     YPEIPIALFSVYIYRMTRSGRPMSALRLALLGALLGSFFWFGLKYNMIFWPLLIVAVL
                     SLRPAVRPRSRILWFVIPALAGLALFYFAVWNMYGTFSPFAVYEGVLEPGQAREIARS
                     FLDLPLSARIETLLDYFLDQRDGLLPYAPFWAFFFLGLVEMARRGRKTRAELFGLLFI
                     AGPYLLNYAFFTHRQGFCPQGRVLAPVSWVAAVALGCFLERRGNRFFTWLFGLGVAAT
                     AAMSLILLGHPDYLYQPTTHDFTARAGDLFVHLGNVRLFLPPFLPSFVKVDNSAYLPN
                     YVWTGLLLLFIAAYAVFAKGGGKPLPRLFPPAAAAVLLAGGLGLWVLFPRVPLHPSRT
                     VVYSTGGTLGFYLKPMGRGVVAKKEGEMYLHFEKSYRFVFASREKLGKIRLAYGSRKG
                     EHEIRMTCFDTPLLEDVTAEEIKETVFAPRAEYRLRDLYLYEIGLTLKKHSGENLLVD
                     PYLLKITPLR"
     gene            complement(19293..20321)
                     /gene="tsaD"
                     /locus_tag="PKV64_03415"
     CDS             complement(19293..20321)
                     /gene="tsaD"
                     /locus_tag="PKV64_03415"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPN16163.1"
                     /translation="MTGKAMRHLLAIESSCDETSASVFGEDGTILSNIVLSQDEVHAP
                     YGGVVPELASRQHLKSITPVVRESLTAAGLPLEAMDFFAVTQGPGLIGSLLVGLSFAK
                     GLAYYHRKPVLGIDHVQAHIESAFLDRPDLPFPVLALIVSGGHTALFLMPRKLRARLL
                     GRTRDDAAGEALDKVAKFLELGYPGGPLIQKLAENGDPGRFPFALPRMSDRSLDFSFS
                     GLKTAAVRIIRESGISREAAALPDFLASFENAVARTLVRNVERAVESFVPRSLVLCGG
                     VARNRRLRAAFERFASGRGLPFGIPSPKLCTDNAAMVGALAWAKIRASRKKIPLRLDL
                     DAYPRSPR"
     gene            complement(20318..21349)
                     /gene="mtnA"
                     /locus_tag="PKV64_03420"
     CDS             complement(20318..21349)
                     /gene="mtnA"
                     /locus_tag="PKV64_03420"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPN16164.1"
                     /translation="MRPTIEWAGDRIIMIDQRKLPLEETYVECRTYEEAAEAIETMVI
                     RGAPAIGVAAAMGVAIGMRAVKKGEDRDAAFARVHDRIHRTRPTARNLFWALERMKSV
                     FEAHRGEPLASLQAKLAAEAVEIDREDEELCRRIGTHGAPLIKDGEAVLTHCNAGGLA
                     TAGYGTAVGVIRAAWEAGRKFTVFADETRPFLQGARLTAWELERIGVPYRVITDNMAG
                     WLMHRGEISAAVVGADRIVRNGDAANKIGTYSVAVLCRENNLPFYVAAPLSTFDFSLA
                     DGSLIPIEERPAAEVREINGRLIVPPTARVRNPAFDVTPARYIAGIVTENGVARPPYE
                     ASLEALRRK"
     gene            complement(21418..22197)
                     /locus_tag="PKV64_03425"
     CDS             complement(21418..22197)
                     /locus_tag="PKV64_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPN16165.1"
                     /translation="MREKAPAPVPVVREIEAKSILNASKIYDYCLNPYTGCQVACVYC
                     YAALFMRRYSGHVEPWGGFVDVKTNAPLLLERQIAKAKRGTIWIASVCDPYQPLEKKF
                     GLTRRLLEILGGRGFPVVIQTKSALLRRDLDIIRRIKDVEAGFSVATEDEAAARLFEP
                     RAAPVRERIEILREFKAAGVRTYAFAGPLLPGNPETLASLLAPAVEHVFIDRMNYQPA
                     VRAFYARHGLLEYLSDAFFRRQGGALARELRARGVRVKTVF"
     gene            complement(22276..24066)
                     /gene="lepA"
                     /locus_tag="PKV64_03430"
     CDS             complement(22276..24066)
                     /gene="lepA"
                     /locus_tag="PKV64_03430"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPN16166.1"
                     /translation="MNSIRNFSIIAHVDHGKSTLADRFLEVTGAVAARHLRAQTLDTM
                     ELERERGITIKARTARLEYTAADGRPYVLNLIDTPGHVDFSYEVSRSLAACEGALLVI
                     DASQGVEAQTLANTYLAVQNDLHLIPVINKIDLVSADPESALDQLEHIVGLSRDEALL
                     TSAKTGKNVKEILEAVVARIPPPKGDPDAPLKALLFDSWYDNYRGVVVLVRVVDGTIR
                     TGDAIMLMGLQADYEVEEIGYLTPDPVKREALSPGEVGYLIAGIKTMSHARIGDTITH
                     THRPASAALSGFKEAKPTVFCGLFPAAETRVEDLREALEKLRLNDASFFYEPESSQAL
                     GQGYRCGFLGLLHREIVQERLEREFGLTLLTTAPSVSYRVKVRSGETLDIRTPSQLPR
                     PQDIVSISEPVIEAMILTPDRYLGAILALLEGRRGEQKKLEFISPHRVLLQYLLPLNE
                     VVFDFFNQLKSASQGYASLDYELAGWREGPLVKLDILINREQVDELSVIVHEDKAYTF
                     GRALVERMRKAIPRQQYEVALQAALGSRVIARETIKAFRKDVLAKLYGGDYSRKMKVL
                     EKQRAGKKRMRTVGRVNIPQEAFLAILEVK"
     gene            complement(24210..25460)
                     /locus_tag="PKV64_03435"
     CDS             complement(24210..25460)
                     /locus_tag="PKV64_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPN16167.1"
                     /translation="MIPRREFLKIGGLTLSLPAFVRGLFASPAGAGDEVGNLTAGVRP
                     LDPADYEERRERAARRMADGGLDAVFLSGGANLAYFTSVSWWTSERTFGAMLNKKGAP
                     IWVCPAFELDRAKETIPSGQEIRTWKEHESPYALIGGILKDLGGRRLGLSPNTAAFQV
                     YGLNRDVSWIEPADAASVTEGCRGTKSAKEIAYMDLAARITKLAYRKAFGRLSEGMTP
                     RDLAGLIGEAHAAMGATGGGGPQFGPGTSFPHGSRAPRTLTDGMAVMVDGGCSVEGYR
                     SDVTRTIVFGKPSDKMIRIWDIVKKAQTAAFRACRPGAACEDVDAAARKVIEDAGFGP
                     DYAYFAHRLGHGIGLEGHEYPYLVRGNKLRIEPGMTFSNEPGIYIAGEFGIRHEDSMV
                     ATEDGARCLGGMEALAIDRPFADE"
     gene            25628..25828
                     /gene="tatA"
                     /locus_tag="PKV64_03440"
     CDS             25628..25828
                     /gene="tatA"
                     /locus_tag="PKV64_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPN16168.1"
                     /translation="MFLFGTVGPQELLIVLLIVIIIFGAKKLPDLGKSLGESIRNFKS
                     SVSGKGKEAGKDESKDKIPPAD"
     gene            25841..26971
                     /locus_tag="PKV64_03445"
     CDS             25841..26971
                     /locus_tag="PKV64_03445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN16169.1"
                     /translation="MLRTIEMLAARRRAAEDDFRRTLAELRRISESLDPLVRRLSAPA
                     ESSGEIRSAVAEAAAALKALAEAAARQAEALDVLSDVRDKLWDAEGNNHVGMIFKSME
                     WRVDRLAIAAEDAAVLLKTFASLQDELHRLLAAVQDKNLSTPALIAPLIEPLEEGRYL
                     RFENRFRGAEEEIRKQQIAYLDDFPPGGRILDLGCGRGEFLELLTARGFQAEGVDLNV
                     EMIALCRDKGLSVEKGDLLECLAARPDASLDGIFSSQVIEHLPPASLRRLVELSYAKL
                     RPEGRILLETVNPLSVFALVHIYFLDFTHRTPVHPQALQFLLEAAGFSSAAVRFGLGL
                     EAEKLGHIPPADEAAAVFNRNVDRLNALLFAPPQYSVLGRKT"
     gene            27163..30219
                     /locus_tag="PKV64_03450"
     CDS             27163..30219
                     /locus_tag="PKV64_03450"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPN16170.1"
                     /translation="MTKRIFATGMLFLALGAAAAAAGPTVQETGTMFGRVTDLDGNPL
                     PGASVSLMGPSLMGMNSSVTSATGQFFFPLLAPGLYEIRVEMPGFKMQIQRGLRVQSG
                     RTMQIHALLEETAVDEEVTVPPGDQRADTRNVKSSSALGSDTLARLPLPRDHFLPWTL
                     VPGAVADVARDRRLVAVEGAAPRSQIVLWEGAMLNDPVSGLPLIHPVDDAVEEIEYAT
                     TGLPASLPATDGAYLQIVGKDGGNDFHGGLSYYSTGAALAQDLDASSAGSLRPATPDR
                     YESYRDLSFHLSGSFMDDRAWMFLAGRRLTSTVSNPYSPETRMAALGFTDSPAFDLAR
                     KEWSGYAKIHVRPTNEVSYSGLLLFNNFTEPYDTVSVSAGTSSDRVPERSPENVLVTT
                     HNIRFSIRQNTAAEFQAHYIHHGFTLASRGSTGAAAVYDAVQDVWWGAPAREGTEKSE
                     AFGVSAALTHFKEDVFGADHDLKIGFSYDQAETHNDWYRANPFNTYWFDYKTKNPYYY
                     DGLSLGRLEVLPAPAAAASWDVSEMTRKLGAYVQDTLTRKRLALNVGIRADFQILSLP
                     LQGRNLTSPAYAPAFFNTALDLKDFMKAIDGLIDEAGVTSPLSSITTLERTSVSFFTL
                     SPRAGLIVDLLGNGRAALKFSFARSHEPLWISGYDEDQFLEPQTLTLIWHDFNSNRLM
                     DMPGTDDYTLLSWRLQNLDSVYYDGVKAPRTDEFTAGLEYGPASNLRLGLKLTYRKTT
                     RLVENIDSVNGSDPLATDAIGRIWLPMTVTDPGADALFGTADDASLTVYGLRADRPAP
                     VWVAANPEDAYRKYLGATLTLEKRMADNWQLQGSFTLSSLRGTADYALPGRINRTFLF
                     NDPNALINTEGPLAFDRPVQFRLSGTYLFPFGLTFSAFFQYYSGAPWARTLTVYFPAG
                     YMGYGTREPSVTVYAEPWGTNRAPGVACLDLHLEKRFTLKKGASLTLMVDVFNTTGRN
                     TQTISQDRAGILDERKTPARYTVTQDERMVNLYGVRQFRVGIKFGI"
     gene            complement(30226..31239)
                     /locus_tag="PKV64_03455"
     CDS             complement(30226..31239)
                     /locus_tag="PKV64_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPN16171.1"
                     /translation="MKKVPVLPAIAAWAALALTVLGSAGCALYQMERELPEKYAEFLS
                     EVRYLITNAERKTFLSTPDSGKDAFIEDFWARRDPDPDTSDNEVRSEYYARIAQANEM
                     FRRDAVKGWLTDRGRIYVLYGPPDEKRVPMLAETSGRNCLEIWYYKGFPIQFLDEMCT
                     GNFRQVTFDLAPLKELSLNQPPAPLGPDGSRPGEPGRRPAQPVSRTRGARAVLDFSAE
                     LRSTVRRADRMEGMLRLEIPFRLIWFKSEGGRFLTTFTVFVRVRDEEKKTVWEKTFSA
                     EANYDETEIHKRDGDVHVIEIPIYVIQEDALARLAAGTPTVEVKLTNQTGSEDVEKSV
                     EWK"
     gene            complement(31310..31999)
                     /locus_tag="PKV64_03460"
     CDS             complement(31310..31999)
                     /locus_tag="PKV64_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPN16172.1"
                     /translation="MKTKPLLNLKSLKEQVYEFLREAMRRGEILPGSVIDMEETSLRL
                     GVSKTPLRDALLQLEMEGFVTILPRRKVVVNVLRPEDIKNYYEIIGALESTALRQAFA
                     LLGGPEFEEMTRLNAEMRDAIGRDDFDLYYEKNLAFHDVFLSRCGNASLLKVVNNLKR
                     RLYDFPRQQGFVKEWEVASIGEHEAFLELVKAGKKDEAAAHIRDVHWSYGYQEKHIAK
                     YYRRASEICKG"
     gene            complement(32074..34170)
                     /locus_tag="PKV64_03465"
     CDS             complement(32074..34170)
                     /locus_tag="PKV64_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPN16173.1"
                     /translation="MKRRSAAVGPAILMLAACLFLGAAGPARPSGTDLSPVHRKWLED
                     DVDGILSSRERDVFLRLDSDRERDLFIAAFWKRRDPVPATPENEFKAEHERRLAYADA
                     HFGRTAARPGRKTGRGRMYVLLGEPDDIVRYSGKDGIREAEVWFYRNEENARTAGLPP
                     SFNLVFYQEGSVGDFKIYSPFQDGPAALLSDFRGDPGDYEKARRALSALDAGLAAVSV
                     SLIPGEASGFPGRPSPSSDILLQKIAASGAGRIDDEYAAKFLEYKDIIEVEYSANYLD
                     SRGTMIVRREPSGTAFVHYAIEPARLSLAAAGREYAATLRLNGAVSLEDGRIIYAFEK
                     IVSVRMDEERKNTLSRVPFMIQDLFPLIPGTFKVSLLLKNEASKEFCAFERTVVVPES
                     GSGPGLTAPLLGHRVRPSGPEPSALKPFLFGSTQVYFQSGPTLVRSDNVVLAFQVLGL
                     DGPARSRARLRFSITRDDVVVEEWERDLSGVPDVVESIPASGLNPAFYTARVRLVIDG
                     REAAAGGGDFAVTPLEAVPRPWIHAKRMPADGDPAYDGLLGRQLEAAGRAAEARPFLE
                     KAVAGRTAGPEIALALARVYDRAGEYAAAAFILDPYLAAGAEPVYDVWILAAETRLRG
                     GDFGGAVGVLDGAMTRFGIHPRLLNLIGDGYLGLNRRAEARAAWERSLALNPGQPELR
                     KKLEALAASPAGGGAH"
     gene            34297..35022
                     /locus_tag="PKV64_03470"
     CDS             34297..35022
                     /locus_tag="PKV64_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN16174.1"
                     /translation="MIELENVSKSYNNGRVKAVDDLTLTVRPGEIFGFLGPNGAGKTT
                     TIKLIVGLLRPDAGTIRIDGIDIRKDPIAAKSVTTYVPDTPAVYERLTGLEYLNFIGD
                     VYGVPKAERLRRIEHWLEIFDLSAAVANPIQSFSHGMKQKIVLIAALLPAPRVMVLDE
                     PMVGLDARSAHHMKEMMRTHCAGGGTLFFSTHIMEVAEKLCDRIGIIDRGRLIAHGTL
                     EELRAIDREGQSLEKIFLELTAE"
     gene            35019..>36064
                     /locus_tag="PKV64_03475"
     CDS             35019..>36064
                     /locus_tag="PKV64_03475"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16175.1"
                     /translation="MKKFFRLVRVGLRVNFGLSLLRPKALLENKRDFWLIVLILVGIA
                     GLSPVLLYYLKGIRFLYGLLAPLGQQAALLTLAILAGQFLILVFGFYYVLSAFYFSRD
                     LDLLVPLPVTPAQVLLSKFSVVVVNEYLTASLLVVPVLAAYGFHSRAGLRYWAGAALV
                     YLFLPVIPLAVVSLLVVGMMRFVNVGRKKDALMIAGSLVLMAAALGLQFWLRSLPASG
                     PGRQALVAFFSEGGIIRKIGASFPPSVWATRALVPEAGASGLGSFLLYAGVSVGLFAA
                     LFLAARALFYRGLLGLGEISARKRRLSREALSARVSSGRRPVRAIFLRELRIMNRTPI
                     FLLNGVLSVVLIPV"
BASE COUNT         6062 a        11808 c        11260 g         6934 t
ORIGIN      
        1 tcgctgtcga cgacgacgat ctcggagacg acgccctcca ggctccggag agcggcctcg
       61 atgttcgcct cttcattata agtgatcacc accgccgaga ttttcacgcc ggaatcttaa
      121 cacggcccgc cgcggcggtt caagaacgcg cggagcggcg gcgcccgcga ttctggtata
      181 gtatcccctc tcgaccgctc gataaggacc gttgtcatga ccgcctacga acgcctgctg
      241 gccaaagcct tcgatcccga ggaattccgc cgggaaggga agaaaatcgt cgatcttctg
      301 gccgatttcc tggccgcctc ggagccggaa ggccggaacc gcgtgttcgc ctgggaggat
      361 ccggaatcca tgttcgcctc ctgggcgggg cggttcgccg cgaaaccgtc gaggccgttc
      421 gccgatcttc ttccggaaat cctcgatcgc tccaacaacc ttctccatcc ccgctacgtc
      481 ggccatcagt gcaccacggc cttgcctttg gccgccctcg gcgggctggc cggccagtta
      541 ctcaacaacg gcacggccat ctacgaaatg ggcccggtca acgcggccat ggaacggcat
      601 ctcatccgct ggatggccgg attggccgga ttcgggcccg gcgcggacgg cgtcttcacg
      661 agcggcggat cgggcggcaa cctgaccgcg ctgctggccg cccggcaggc cgcggccgaa
      721 ggcgatatct ggagggacgg cgcggcgggc gcggctcccc tggcgattct agcttcctcc
      781 caatgccatt actccgtccg gcggtcggcc gcgatcatgg ggctgggcga ggaggcggtc
      841 gttcccgtcg atgtcgatga aaaattccac atgaccgcct ccggtttcaa gacggccctg
      901 gccgaggccg ggaagcgcgg ccggagaccg ttcgccctcg tcgccaacgc ctgctcgacg
      961 gccaccggca gctatgacga cctcgaacag gcggccgatt gggcggcctc cgccgggctt
     1021 tggcttcacg tcgacggggc ccacggggcc ggagccctgc tgtcgcccaa gtaccgctcc
     1081 cttctccgcg gcttggaacg agcggattcg tttatttggg acgcccacaa gagcctcctg
     1141 atgccggccc tggtcaccgc ggtcgttttc cgcgacggcg gacggtcctt cgaggcgttt
     1201 tcccagaacg cctcctatct cttcgaaaaa accgcgaccg aggaatggta caacttcgcc
     1261 caccggacgc tggaatgcac caagacgatg atggggatgc gcctgttcat ctccctggcc
     1321 gcctatggca cggatttttt cgccggatac gtcacggccg cctgggactt ggcccgggag
     1381 ttcgcccggc ggctccggac cgccggcgat ttcgagacgg cgtccgagcc cgaaagcaac
     1441 atcgtctgtt tccggcacct gaagaaaggc gaaccggatt tggacggcct ccagcggcgg
     1501 atccggagga ttctcctgga gcaggggcgg ttctacgttg tccaaaccca gctcgacggc
     1561 cggacctggc tgcgctgcac gctcattaat ccgcggacga cggccggcga cctggacgcg
     1621 ctgatggaag agatccggtc ggccgggcgt tcctgaaact ggatccctct ccctccggaa
     1681 aacggggacg gaagcaccgg tcgcgccgga ccttgaatag cggcggcggc gaaaggacga
     1741 gcggttttct cccgcgcgga tatgataaga tattcccgcc atggcgggtt cggatgaatc
     1801 ggtctttctt tccgtggtcg tcccggccta taacgaggat gcgcgcctcg ggccgaccct
     1861 aaggacgatc cgggctcacc ttgaatccag gatcaacgcc gccgggacgg ggaacggacc
     1921 gacggaaatc atcgtcgtcg acgacggctc gaccgaccgg acgggcgccg tcgccgccga
     1981 agccctccgc ggccggcccc gcgaccgggt tctccgccga gggaaaaacc gcggcaaggg
     2041 tttctcggtc aaggaaggga tgctcgcggc cgccgggcgc ttgatcctgt tcaccgacgc
     2101 cgatctctcc accccgatca ccgaactcga aaagctcctg gccgggatca gggacggtca
     2161 cgacatcgtc atcggctccc gagccctgcc cggggcggag atccgccgtc gtcagcgtgt
     2221 tctccgcgag aggatgggga aaacgttcaa ccttttcgtc cgcggatggg ttctcaaggg
     2281 attcccggac acccagtgcg gtttcaagct cttccggcgg gaggccgccc gagatatttt
     2341 ttcccgcgtc cgcctggacg gcttcagctt cgacgtcgaa gttctcttcc tcgcccgccg
     2401 gctcggctat tccatcgggc aggtcccggt cgtttggatc aactcgcccg aaagcaaggt
     2461 ccggatcgtc gcctcgtccg tttccatgct ccgggatctt ttccggatcc ggcgtcttca
     2521 ccggaacgcc gggttccggc gatcaagctg aatacgcagg gaaggccggc cggcggccgc
     2581 cgggagagaa ccttatttca ccgcgggcga ggggcggagt tccggccccg acggcccggg
     2641 gcgggagccg aaatcgtcct ggcgggccga gcggcggtcg ccagcgaagc cgcgcccccg
     2701 tccgaatccg cgggagcgct tccgctcctc ccgggccctg agctcggcct cgaagccccg
     2761 atcggaaaca aagagatccg gcgtcgccgt tttgatcgga atccgcatcc cgatgtaggc
     2821 ctcgatttcg gaaagcttgt cgccgtactt ctcgcaggcc agggaaaccg ccttgccttc
     2881 cttgcccgcc cggccggtcc ggccgatgcg atggacatag ctttcgatgt cctcgggcag
     2941 gtcatagttg acgaccatct ccaggccctc gatgtgaagg ccccgggcgg ccacgtccgt
     3001 cgcgacgaga aacgggaacc ggccggcgga gaagtcgtcg atgatcctca gccgtttgtt
     3061 ctggggcagg tcccccgtca gaaaacggcc tcgataaccg ttcttttcga gctttttggc
     3121 cagccgctcg gcggcgtggc gggtgttggt gaagatcagg gcgtttttcg gcgcttcggc
     3181 cttgatgagc cccaggagaa ggttggcctt gagatggctg ccgacccgat aaagctcctg
     3241 ggagatcgcc tcgactgtga tccgctcggg cgtcagctcg atgaacacgg gatcgttgag
     3301 aaattccacc gcggtccgcc gggccagccg gttcagggtg gcgctgaaga aaaggctctg
     3361 ccgccggccg gccggcggga gcttccggac gatcctcttg atgtcgggaa gaaatcccat
     3421 gtcgaacagg cggtcggcct cgtcgatcac caggatacgg acgtcgccca ggcgcagcgt
     3481 tcctttctcc tcgaggtcga gaaggcggcc cggcgtgccg atcaggatgt cggccccctt
     3541 gcggaggacg gcctcctgct gctcataacc gacgccgccg taaaaacagc cgatggtgaa
     3601 accgaggcgc cggttcaaca gatcggtttc gccctcgatc tggacggcca gctcccgggt
     3661 cggggtgatg atcaaagttt tcccgctttt ccgggatccg tcccgcagca ggcggtcgaa
     3721 aatcgtcacc aggaaggccg cggtctttcc cgtgcccgtt tgggactgga cggccgcgtc
     3781 acggccggcg agggtctcgg ccagcgtccg ggtctggacg tcggttgtct cggtataccc
     3841 cctgtccgcg atcgcgcgga ggagccgggg atccaagctc agttcgtcaa aacgcattaa
     3901 aaaatccttg agaattgaat ttcaacataa ggggggaagt caagcggcgt atctcggaag
     3961 ttccttgaat cgactcgttt gcactaatac cggccagatg ttaaggagga aatatacatg
     4021 aaaacccccg ccctggcaag agaaaattca gaaggaaaaa tattgggatt cggagaggcg
     4081 ccttgtgcta aattagaatc atgccccccg gaaaaagctt tcccctcctc cttggagccg
     4141 ccttaacggc agcctttctg gccgcttctc ctccggccgc cgcctccgtg gagatccgga
     4201 tcggcggcgg cggctggacg ctcgcgccct tccatccctt gatcgaatcc cgcagcaagg
     4261 aggctgtcca agccgaattc aacgctttcc tcgaggccgc gcttccccaa ggaatgcttt
     4321 cgcccgtccg ggcggacatc gacctggcct cctcgggcgg ctcgctgttc gcggagcttt
     4381 ggcttccgct cggccggggc cggttcgccc tcggcgtccg gggcgatgtt ttccgcttcc
     4441 ggattccttt caccgccgcg gcctcggaaa cgattcagct tatcggattt cccctcgcgg
     4501 agctgaacgg gacggcggcc gggacggtcc ggctcgaggg tttgggactg tccctcctgg
     4561 ggcggtggac ggttgtatcg gccggatctt ttgaagtcgg gctgcgggcc gggatctcgg
     4621 ttcttccctt ccggggacgc ctctcggccg acgggattct ccaggcccgg accatcctgg
     4681 gaaatttcac gtacgccggc ggcctggagg gaacgatggc cgaaatccgg gaaggagggg
     4741 ccgacgtgcc gtccctggtg ctcgccccgg ccgccggcct cgaaacggcc ttccgccttg
     4801 accggcgctg gaggatcttc gccgccctca ccgtttccca cggcacgttt tattcagcgg
     4861 gtctttcggg ttctttctga cttcggctta tgcccggccg tttcgggccg caaaccaaag
     4921 accgggagga tccggctcag gacaaaagag cttcgatcgc ggccaaaagc tgattgaagt
     4981 cgaccggctt ttccaggcag atgaacgggg cgagagaaag cgtccgcccg ccgcatattt
     5041 cttcctttgg ataaccggtg atgaacacga aggggacggc cccttccgcc cggaggcggg
     5101 cggcggtctc gaccccgtcc atggatccgg ccaagtgatt gtccatgatg atgacatcga
     5161 agcgctctcg tcccgcccag gcgagagctt cctcccccgt ggccacgacc tggacgcggc
     5221 cgcagccggc gtttttcaag cgggcgctca gatccatggc gatgagggcc tcgtcctcgg
     5281 ctaaaagaac ggccggctcg cgcttcatgc cccctcctcc gcgaaaaagg ctttggaggg
     5341 aaagcggatc aggcaagtca cgcctttttc cgtctcgaac cggaccgcgc cccggagttg
     5401 atgctcgacc agcatgacca gagtctgtaa cccgagcgtc gcttgggcta aaaggtcgaa
     5461 tcccggaggc acgccgcggc cgttgtcggc gaaatcgagc tcgatggttt ttccgtcggt
     5521 cgaccgggcc aggcggatcc ggatttcgcc ccggccggag ccggggaacg catgcttata
     5581 gacgttggaa aacagctcgt tgaggatcat cccgcagggg atggcgacgt cgatcgggac
     5641 cggcagctcc tccagatcca gcttcacctc gacggccgcg gccgcctcgg ggtggctttc
     5701 cgccagctgg gcggcgaggt cggaaacgta gtccttcaga tcgatgcggt ggagatcttt
     5761 cgactcgtat agcttccggt ggaccagggc catcgcccgg atcttgtcct cgatgtcccg
     5821 aaaaacggcc gcgatccttt cgtcgtcgat cgcccgggac ttcagcgaca gcatggcccc
     5881 gatgacattc atgttgttct tggtccggtg atagatttcc cggaggaggg tttccttctc
     5941 ccgcagggag cgctggattt cctcctcggc ccttttgcgc tcgccgatgt cctgaacgat
     6001 cgcctggacg tggatccggt tattcaggac aaggccgttc aagcagacct cggtctcgat
     6061 cagcccgccg tcttttcttc ggtgccgcca gtaaaaccgt tgcggcgcgc cggcgaccgc
     6121 cgcggcgata taggcccggg ccttttcgcg ggaatcgcgc ccgtcaggtt gcgcgggagg
     6181 agagaattcc cagggcgaat ggccgatcag gtcctccttg gaggaacacc cgtagagcct
     6241 gaccgatcga tcgttgcagt cgatgaacat ctcatcgatc atgaggagaa tggcctcgtc
     6301 cgaggttccg aaaagcgtcc ggtatttctc ctcgtcctcc cggagcttga tttccagccg
     6361 cttccgctcg gtgacgtcct gaaggatcac cagaacccgg aacggcgttt tcccgccccc
     6421 gcggaactca ggaacggcgt cgacgatgag gcggcggacg ttcccggacc gggactggat
     6481 cactcgaacc cggacgcctc ggacgggccg gccggttcgg agggcgacct tcgagggatg
     6541 atcggcgggg cggagcggaa ggccgtcctc tccggtcaag atccagcctg gacgggacgg
     6601 atcaggggaa aggagttcct tccgggcgca ccccagcatt ttcaaggccg cgggattggc
     6661 gtcgatgacg gcgtcgcggg cgtcccggta cacgacgcct tgggtcaagg atttgaaaag
     6721 aacgctgaat tttttcccgc attccccgtc cggcgccgag gcccctgcat tcctcagggg
     6781 aaccgtcttc ctctccgaac ggcgatccgc ccggaggggc ttcaaagagg cgctttttct
     6841 ctcctttaag gacggagcgc cgccccggtt cccttttttc acgccctcag ggtaatcagg
     6901 ctccccgcga aagtcaaggc agacggaccc cgcgccggac gaggctcatc cccgtggcct
     6961 tctccaggcg ttcgacggac aggatgtagt cgatcttggc cttgagctcc aaggaccggg
     7021 cattagccag gcggtcctgg aaatccagga cgaagtagtt cgtcgacatc cctacggcca
     7081 gttttttctg ctccgcctcg aggctttttt ccgccagctc ccgagccaga cgataggcct
     7141 cgatccgctt ggcgtcggtc tcgaccgctc ggacggcgtc ggcgacgtca agggcgacct
     7201 gccgctcgag gctcttcagc ctggcttcgc tctgggcgag gtcgatccgg gcaacggccg
     7261 cgtcggccct tgtcaaagca tggcccaggg gaaaggagag cgtcaagccg acgctccaat
     7321 tgctgtaccg gaaccgggcc gcatccttcg tcgcgtctcc cgcgcttccc ggctccttcc
     7381 cgatgatgat tccggtcatc gggttgtcgt cgaggtactt gatccggtcc cccgaaatcc
     7441 ccgggctcat gtaagagaat tcgaaattca aggcgggcag catctggttc cgggcgaatt
     7501 ccaagctgag ggatttattg tcgatggtct tgcggagggc ttggagatcg gtccggttcg
     7561 cggcggcttg tttcaacgcc tcgtccaggc tcaaggagac cccttgcgcg accgggcggt
     7621 cgacgggctc gaggatcgtc cccggagctt cggccggaag attcaggatc gttttcagca
     7681 cctcgcggga gcgggtgacc atcgcttcgg cctggatcaa gtcggcttcc ctctgggcga
     7741 cggccgcctc ggcgttgagg atctcgatcg gggccagctg gccgaattcg acctccttct
     7801 tgtttttggc caggaggtcg cggcccagct cgagcgactg ccgcttgacc tggaggctgt
     7861 cgacggcgta aacgaaattc caatacgctt cctggacgag gtaaatggtc tccatcatcg
     7921 ccccgcggag ctggacatcg gcgatctcga ggctgttttc agcctccagg atctggcggc
     7981 gggcgatctt gagcccgaaa tccttgagaa gcggctgact gaaagacaca gccagcgtcg
     8041 tcccgaagcg ggggttgatc agctgaaagg attgattcgt ctccgaccga tatccttgga
     8101 aggagagcga aaagcggccg ccggtcggga tcatctgagc gatccccgcg gaatagttcc
     8161 ggtatttatc gatgctcgtc ccttcgccct gcagccacca gtacgagggg ctttcccggt
     8221 gatcgtcgcc gaagctgaga tcgaattgag gcaggaaggt ctcccgggcc ttgaaaagct
     8281 gtccttccgc cagctcggga ttgtaggatt cgacggcgat gtcgaggttg tttttaagcg
     8341 cccgggcgat cgcttcgtcc agggtcagcg gccaaacggc tccgtcttca gcggccagcg
     8401 gcccaaaagc cgcgccgatc gttaacagag cgagaatgat ccggcggatg ttcacggcaa
     8461 acctccctga gatccgggtc gcgccgtttc cgtttcctcc ggaaagcgag gaaaggcggc
     8521 ggtccggccg tctcggattc atacggaaaa actgaggcgg ggttcagcgg ccgggctccg
     8581 gcggaggcgc cggcgtcgtc ccgatcgaaa tcgaatagac cgccaggccg aggcggcggg
     8641 tgtcgctgtt tccgagcgtc tcgtccggga cgaaggaggg cgattcgatt tcgatccggt
     8701 aaaggcggtc gctgagaaaa tcgaagcccg cttcgaagac ggccgtccgc cccttccgcc
     8761 cggccagggg ccaagacgcg gagacggcgg ggaaggcgcc ggcatcccgg tcatacgccg
     8821 acaccgccag ccggagaggc gcatcgtcat cgaaatcgga ccggaatgcg aggcggatcc
     8881 tccgggcggg gaggcgggcg tagacatacg cccaaccctt tccgtcggtc cagcggccgc
     8941 ctacgccgtg caatccgtct tcgcggggcc agaagccgcg gccgaacttg tcgaccgttt
     9001 gttccgcccg gtccggaagg agggtcgttt cgaagacgaa aagattgccc cggcaccgac
     9061 ggcagagcgc ctcggccatc gcatcgcggc ggacggccgc ccgccgttcg gctcggagaa
     9121 catcggccac cggcgttcgg tcgatgtgac ccaagtccat ttccccttca taatccaggc
     9181 agcaaaggac gaaatttcct ccggccaaca ggcccaggtt gccggtcatg gggcaaggcc
     9241 gcggctcgag gcgctcctcc acgtaggtga accgccccgg gaaagcggtc tgaaggaacg
     9301 tccgatcccg ggtccagagc tccagggatt tgaacacgag gaagacgtcg ggcaaggcca
     9361 tgtagcccca gaagacctcg tccttcagcc ggtggaaacc ggggggcagg ccggcccggt
     9421 ccacggcgat tttcgtcccg acatgatccc ggtatttcac cgcggccgtc tcccgggcgg
     9481 aagcgaagga atcgggatat cttcttttca tccgcttggc caggcgttcc agccgccgga
     9541 gcgtccgggc gatccaccgc ccctcggctt tttcatccgg gaaattggcc cagagcccgg
     9601 ctccgccgtc ggcctggatg gtcgcgtcgc ggctcatgac gtaattcgag gcgacatgca
     9661 tttcgagccg ggcgccgctg ttcagccgga acttgtcctc gatgaccttc cagagaatcc
     9721 gctcgtaagc ccgaaacggc aggcggctgt atccccgcag ggcatacgag cggggcgtgg
     9781 ccgtctgaaa gctcaaggcc aaggtcaggt tgccgtggcg aagcagctcc cggcgaaggg
     9841 cgccgtcgcc caggagggtc atgttcgtga tgagggtgac cggatgcccg gccgcctcca
     9901 agtcgtccag gagcgggtaa accttcttgt tgaggagcgg ctcgcccaaa atgttgagaa
     9961 ggacgggggg acggaggccg aggtcatgga gctggccgag gaaactcttc aggcgagctt
    10021 cggacaagtg ctccttcggc cggcggagga catccgaagg gcagaacgcg caatgcatgt
    10081 tgcaggcgct tgtcagctcg aggaaaatcg tccccggcgg atcggggatc gaggcggccc
    10141 ggtcgttcat ggcggactca ttatagacta aaatgccgtc gccggcttcg gggcgcgttc
    10201 ttgacttgtc cgtcggtccg acatacagtc ttggcgatga aaatcctccg ccgcatcctc
    10261 cggcctatcg tctttttcgt cctgtcggcg gggatcgcgc tcattctccg gctgaacgtc
    10321 caggccccgg atttcaccat ccgcaaccac gccttccggc ccgaagccgg tcctctcctc
    10381 gtgtccgcca tcctgatctt catccttctc gccgggctct gggccttgat cagccagatc
    10441 tggacgaaaa acagccggct gagctacaag gaagcgctgg ccctcgattt tccgacctat
    10501 ctgccggcgc tgttcttcct cctggctccc ctggccttgg tgcattacct gacccgggac
    10561 gatctccggg aaaggcttgg acttctggcc gcgggcgtcc tcttggccgt cctctatctc
    10621 aaggctgtcc gcgtcgccca ggccgaccgc caagggaaat cgaattgggg ggccgccctc
    10681 gaccgttttc tcgcctggcc cacccggcgc cgcctgatcc tcttgtccgt tctcgccctg
    10741 ctcctgatca acggaggcgc gctcctcgtg agcggaaggg gcgtgtcctt cggcggcgac
    10801 gagccccatt accttctgat cacccacagc ttgctcaagg acggggatct ggacctggcg
    10861 aacaattaca aggaccggga cttcgaggcc tacatgcccg agggcgtcgc gatgcgctcg
    10921 cacacggtgc ccgggaaaaa gccggaaagc cgctattcct ttcattcgcc cgggatttcg
    10981 gttcttcttc ttccgtttta cgccctcgga ttggccctgg gcaaaagcgc cctggtcttt
    11041 ctcgtccgtt tcgccctgag tctcgtcgga gtctttttcg gaatccaggt ttatctctat
    11101 gcccggcagg cctgggcccg ggaacgcctg gcgttcgccc tctgggccct ggtcaccctc
    11161 tcggccccgg tttttttcta tgccttccac atctatccgg agctggtcgt cgccgcggcc
    11221 ggaatgtatg tgttccgccg gctgcgcttc aaaccggccc gccgggccgg agactgcgtc
    11281 ctgatcggga tcctgatcgt ctcgttcgtc tggtttcacg ccctgaagta cgccttcatc
    11341 caggcgcccc tcttcctcta cgcgatcctc cggatctgga aaggaaatga tccggccgat
    11401 cggggacgcc gcctggccgc cctgatcctc ccggccggcg tcttcgcggc cgcctatttc
    11461 gccgtccagc acgccctcta cggctcgctc aacccgacgt ccgtctcctg gcagggggcc
    11521 atggacggga agcagacgct gagcttcctc aaggagctgt tcaccggcat cccgttccgt
    11581 ttccgctggg agaccctggc cggatatttc ctcgaccagc gcgacggcct cctgctttat
    11641 tcgcctctct atctcttcgc ttttctcggc tgcgtctccc tcttccggaa aaaagccggc
    11701 gacgccggct ggctgctcct catcgccctg ccctacgtgc tcgcctccgc gttcctgacg
    11761 cagcggtcgg gatacgcgcc gcaggcccgg ccgctggtgg ccgtgatctg ggttccggcg
    11821 atcttcctcg gcgcctttat cgccgagggc cggaagcacg cgttccgtta tctcttcaac
    11881 ggggcggccg ggctgacgct cttgatgacc tggcttctct gccgccaccc gtacgccctc
    11941 taccaggaaa cgaccatggg cgtgaccgag cgggccggaa gcttgttcat caccctgagc
    12001 aatctccacg tctatcttcc ccggctcctg ccctccttca tcaaggtcaa ggaaaccggg
    12061 tggacgccga acgccgtttg gatcggcgcc ctggtcctct tcgttgtcct tttcgccctc
    12121 cgccgcccgt ccgacaagcg cctccccttc ccgggacacg cggctgcggc cctggtcctg
    12181 ctggccctat tcttcgttca gtacgtttat tatccccggc ccgtcctgat gtcgccccgg
    12241 ccgatcgtcc acgcttccgg cgagccctgg acgttctatt ccctctcccg agtggcgatc
    12301 atgaacgagc ccgggcggtt cgacattctc caagacaacc gggattatga tttctacttc
    12361 acctcgaacc ggcctctgga aaagctcggc gtggaattcg gatcgcccca cggcgactac
    12421 gccctgcgac tgaacatcgc cgacgcggcg atcttttccg tgacgacgcg ccgggaagtg
    12481 atgacccgaa cggtcgagtc cccccccgcc tatccctgga aaggccggtt cctctaccgc
    12541 gtttcgatcc gcctggaaag aaaatcggat gtccggacgg ccgtcacgcc ctacctcttc
    12601 gctttgagcc cggcccgctg attccagccg cccggcggag gatcacgccc cgggcttcgg
    12661 ggcgggcccc ggcaactcga acatcgggta ccggatccag ccttttttgt gaagcttgaa
    12721 cttgacgagc atcctcaaga tggaaaggcc gtagtggatg ctcccccagg gcccgacctg
    12781 ggaggcctcg gggaaatagc gcgtctcgat gggaatctcc cggatccgca tcccggccag
    12841 aacgccctgg gcgatgattt cggaatcgaa gacgaagccg tccgagttcg cccggaagtc
    12901 gaccgcctcg agataccggc gggaatacgc ccgcaagccc gagtggtatt cggtcaggta
    12961 catgtaaaag gcgatgttct cgacggcggt caggaagatg ttggagaggt acttccattt
    13021 cggcatgccg ccctcgagcg gccggccgcc aagcatccgc gaaccgaaga cggcgtcgca
    13081 ggcgtcgtcg agcagcggct ggacgagctg ggggatgacc ctcggatcgt attgatgatc
    13141 cggatggatc atgaccatga tctcgccccc gagcttcatg gcctcggtgt agcaggtttt
    13201 ctggtttccg ccgtatcccg tgtttttttc atgaacgaat acccggagcc ccaggcggcg
    13261 ggccaggtcg accgtgccgt cgcggctgcc gtcgtcgacg aggatgatgt cgtccaccca
    13321 ggaccggtcg atgtcgtcaa gcgtccggat caacgtcttt tcggcgttgt acgccggcat
    13381 gacgacgacg actcgctttc ctcgtttcgc ggttttcatg acgccttccg gtccggaagt
    13441 cgtcctctta tatcattttt ccgcgtgttt gtcttgaagc ggccgctcgt ccgccccgga
    13501 ttcgagggga cccgtcctca tcccccgccg tccggccgga cctccggcgg accggccggg
    13561 gccccgatca acgacttgaa ttcctccgcc ccgatcgttt cgacttccag gaggcgggcg
    13621 gcaacccggg ccgcaacctc ccgcttttcc tcgatgaaag cgaccacctt gtcgtacctc
    13681 tgcttgatga tccgggtgat ctcctcgtcg atatactgct gggtgaattc ggagtactcc
    13741 ctttggccgg cgatcggctc cccgggcagg aagatcgggc ttctctggga cggcaggacg
    13801 acgttggtga atttttcgct catgccgtag tcgctgatca tcttgcgggc gatgtcggtg
    13861 gcccgggcga tatcgttaga agcccccgtc gagatctcgc cgaagaacac ctgctcgctg
    13921 gcccgtccgc cgaggagaac gtcgatcttc cccagaagct cgtcctccgt caggagaaaa
    13981 cggtcctcgg tcggcagctg aatcgtgtat cccaaagccc cgatcccccg gggaatgatc
    14041 gagaccttgt gaacgggatc ggcgcccttg gcgtaggcgg ccaccagggc atgcccggtt
    14101 tcgtgatggg cgacgatcga ccgctccttg ggattgagca ggcggttctt tttttccagc
    14161 ccggcgacga tgcgctcgat ggcctcgtcg aaatcccgct gcaggacgct ggcccgcccc
    14221 gcccggacgg ccaagagggc cgcctcgttg acgatgttgg ccagatccgc cccggtgaag
    14281 cccggggttc cccgggcgat tttgtccagg tcgacggacg gatcgagctt cacgttccgg
    14341 atgtggactt tcaggatgtc cacccggccc ttcaggtcgg gccggtcgac cagcacctgg
    14401 cggtcgaagc gcccggggcg gaggagggcg ggatcgagga tttcgggccg gttggtcgcc
    14461 gagagaataa tgacgccgga ccgggagtcg aacccgtcca tttcgacaag gagctggttc
    14521 agggtctgct cgcgctcgtc gtttccgccg agagtgatcc cgcggctctt gccgatggcg
    14581 tccagctcgt cgataaagac gagacagggc gctttctccc gggcctgctt gaacaggtcg
    14641 cggacccggg ccgccccgac gccgacaaac atctcgacga attcggcccc gctgatccgg
    14701 aagaacggaa cctgggattc ccccgcgacg gccttggcca agagggtttt tcccgtcccg
    14761 ggcggaccga ccagcaggac gcccttgggg atgcggcctc cgatcgcctc gtacttccgc
    14821 ggattcttga ggaaatcgac gacctcgacc agctcggcct tggcttcgtc cacgccggcg
    14881 acgtccttga aggtgacgcc cgtgtctcct tcggccacca gcttggcctt gttcgccccg
    14941 aaggacatca cgccctggcc cgcgccgccg attctcttgc tgagataatt ccagaagaaa
    15001 accatcaaga cgatggggaa cacccaggag aggagggcgc tcagccagga tctgttctcc
    15061 ggggcgaccg cgtaataatc gacgcctttt tcatccatga gagaaacgag atccgcatcg
    15121 gccatcagga cggcccggta ggttatcgaa gccgcagccg gggccggctc gccgggctcg
    15181 ggtttcccgg gagccgtctc cggggacggc ttccgggtga atcccgtcac gtatttttcg
    15241 ctgatttcga cccggacgat ctcccccgat ttgatgcgag ccttgaaatc gctgtaatcg
    15301 atggattcgt acttcggccg caggagcacg gtgttgatca ggaaaagggc aaccagggtg
    15361 aacaggaaat accagatgga aaatttaaac tgcttgcggt ccaggggttt gaagagggga
    15421 tcgttcttct tgagccccgg atcttctttg ttctttccgt tgcgggcctg cagaatttcc
    15481 ttcttctcgt cgttggcggc catgccgtcc tcctcccttt cttgcgggtc gagcccgccg
    15541 ccggagcttc cgccaggcgc ggggaagcgt cccggtttct ccgtctcccc ggcgccgggc
    15601 cgggaatcca tgatactccg gagggggagg cgacttcaat gcgtcgtcgg cgaaggcccg
    15661 gtcggccgga tggaatttcc cgcgggctcg cccgacgggt ccgcgatccg ctcgaaaagc
    15721 gtctccaaat tcagcgtcag cccgttctcc cgcccctcaa gaaaggcgat gaagcgggat
    15781 cccccctcgt aaaaagccac ccgggtcaag gggagagcca agccgccgtg gttctcctcc
    15841 atgttcagat gccggagtat cgcattctcc aagtaccggg cgaagccttc tttccactcc
    15901 tgccagcaaa ggtactcgta gttttcgggt ccgaggaggt ccttcagccg agcccggatc
    15961 cggagagcct ccgggccgtc gccccggtcc atccggtcca aggcggcggg atagtcttcg
    16021 gcgaactccg cgttttcgta ggggaacggg tagctcagct cccacatata atcgcccgcc
    16081 tccatggcct ggcgatagat ggaaagccgt cccttcaagg ccggctcgac ggtctcccac
    16141 tggcggcaat ggacgaactc gtggaagatc gtcacatacc cgcgggcgga atcgaagacc
    16201 tccgggctta ccacgcaggc gctccggttg tcgagggctt caaggggaaa cgccgcccgg
    16261 acgcctttcg gcatgggcat ggggacgggc gcctccttga ggaagcgata tccggttttc
    16321 gattcatcca gatcaaagat caggaaacgg tcgccgtcga cgacggccac gggataaagg
    16381 ttttggagaa cggggtgaag gggaccgacc tccgcttgaa gcccgatgat ccgttgaagc
    16441 ttgggcgcca ggctttcctt caaatcttcg gcgacggccg aagcggccaa ggcgatcacg
    16501 gccgcgacgg ccgcggcggt catttttctc atttcttcac tccttggggc gcgtccggat
    16561 cgctccgtcg gcgatattca tccggcccgt ctatgatccg gcgcgcgacg attcccgtta
    16621 acgccgctca ttttgatcca atcccggaac aaccgcaacc cgcggccggt ttcggcgacg
    16681 aggcgttcct taatgtcaac gatagacagc cgggaaaagt tctctttcgt ccgcggacgc
    16741 cggccggccg caaaaaggga acggccggaa gcctcgaaag caggccttcc gtcccccggc
    16801 ctcgggcgcg gttccggctt gatggattcc ttcgtcttct tgacaccgcc ggacaagtcg
    16861 attattactg ccttcatgac ggccgtcccg gaatccgcgg tttcccgccc ggttctatgg
    16921 gcctcggccg cggggctggc cgcttatgga acggcctggt tccgggcggc cggggcgacg
    16981 gtattcgatc acggattccg gccggtagga atcaccgcgg ccttgttcct ggggctcgcg
    17041 gccgcggccc tcattctcct ctcccgggcc gcccggtctt ggcaggacga ggcgttcgga
    17101 ttcggagggc cgttatggcc cccggccgtt cttctcgccg ctcccttcct gaccatcgat
    17161 tatttcgacc gggacgacct tcagaggcgg ctccttcttc tcggtctgct cgcggccgcg
    17221 gcgatcgtct acctgggcgt cgccggaccc gcttcccgcc tggaatcgtc gcggccgcgg
    17281 gcgacccgtc tccttgacgg atttctccgc ctgccggtca agcgccggct tcttgtcctt
    17341 ttcctgaccg cttttctcgt ctacaacgcc tgcgccctcg tcctcgtttc gcgggggatg
    17401 accttttcgg gcgacgagcc gaactacctt ctgacgaccc acagcctctt ggccgaccgg
    17461 gacatcaatc tcgaaaacaa ctaccgcaac cgcgattact tccatttcta cgacgagaag
    17521 gagaatccga atctgcggat gaatccctac gcccgggcgg gggtcaaagg ccgcggatat
    17581 gtctacccga tcaacctgcc cggcatctcc ttcctgatgg tccccttcta cgccgccggg
    17641 caggccgttc acggcttcgc ccgaaccttt ctcctcaagg gaagcctggc ggtctgggcg
    17701 gcgcttctcg gcctccagat ctacctcctc gccaggaagc tctggaagcg ggaagggctg
    17761 gccctgggtc tttgggcgct cgtttccttc accccgcccg ttcttttcta cgccgtccat
    17821 ctctaccccg agatcccgat cgccctgttt tcggtctata tctaccggat gacccggtct
    17881 gggcggccga tgtccgccct ccggctggcc cttctcggcg ctctcctcgg atcatttttc
    17941 tggttcggcc tgaagtacaa catgattttt tggccgcttc tcatcgtggc cgtcctctcc
    18001 ctccggccgg cggttcgtcc ccggagccgg attttgtggt tcgtgatccc ggccctggcc
    18061 ggcctggccc tcttctattt cgccgtctgg aacatgtacg gaacgttctc tccgttcgcc
    18121 gtttacgagg gcgtcctcga gccggggcaa gcccgggaga tcgcccgttc cttcctcgat
    18181 ctgccgttat cggcccggat cgagaccttg ctcgattatt ttttggacca gcgcgacggg
    18241 ctcctgccgt acgcgccgtt ctgggcgttc ttcttcctgg gcttggtcga gatggcccgg
    18301 cgcggccgga aaacgcgggc cgagcttttc ggcctgctgt ttatcgccgg gccgtacctt
    18361 ctcaattacg cctttttcac tcatcggcag ggattctgtc cgcaaggccg ggttctcgcc
    18421 ccggtttcct gggtcgcggc cgtggccctc ggctgttttt tggagcgccg gggaaatcgt
    18481 ttcttcacct ggttgttcgg tctcggggtc gcggccacgg ccgccatgtc cctgatcctc
    18541 ctcggtcatc cggattatct ttaccagccg acgactcacg acttcacggc ccgggccggc
    18601 gaccttttcg tccacctggg caatgtccgc ttgttcctgc cgcctttcct gccttccttt
    18661 gttaaagtcg acaattcggc ctatctcccc aattacgtct ggacggggct gctcctcctg
    18721 ttcatcgccg cctacgcggt cttcgccaag ggcggcggaa agccgctgcc ccggctcttt
    18781 cccccggccg cggcggccgt cctgctcgcc ggcgggctcg gactctgggt tcttttcccg
    18841 cgcgtccccc ttcatccgtc ccggacggtg gtctattcca ccggcggaac cctgggtttc
    18901 tacctgaagc cgatgggacg gggcgtcgtt gccaagaagg aaggcgagat gtacctccat
    18961 tttgaaaagt cctaccggtt cgtcttcgcc tcccgggaga agctcgggaa gatccgcctg
    19021 gcctacggct cgaggaaagg cgaacacgaa atccggatga cgtgtttcga cactcccctg
    19081 ctggaagacg tcacggccga ggaaatcaag gaaaccgtct tcgccccgcg ggcggaatac
    19141 cgcctccggg atctttacct ttacgagatc ggcctgacgc tgaaaaaaca cagcggggaa
    19201 aaccttctcg ttgatcccta tcttctcaaa atcacccctc tccggtgacg aggcaacgcc
    19261 cgaggaaagc cggggatgaa aactccccgc cctcaacgcg gcgagcgggg ataagcgtca
    19321 agatccaggc ggagggggat ctttttcctt gacgctcgta ttttcgccca ggccaaggcg
    19381 ccgaccatcg ccgcgttgtc cgtgcaaagc ttgggcgagg ggatgccgaa gggcaatccg
    19441 cgcccggagg cgaaccgctc aaaagcggcc cggaggcggc ggttgcgggc gacgccgccg
    19501 caaagaacga gggatcgcgg gacgaagctt tcgacggccc gctcgacgtt gcggacgagg
    19561 gtccgggcca cggcgttctc gaagctggcc aggaaatcgg gcagggcggc cgcctcccgg
    19621 ctgattccgc tttcccggat gatccggacg gcggccgttt tcaatccgct gaagctgaaa
    19681 tccaggctcc ggtcgctcat ccgcggcagg gcgaacggga agcggccggg atcgccgttt
    19741 tccgccagct tttgaatgag aggaccgccc gggtatccca gctccaggaa cttggcgacc
    19801 ttgtccaggg cctcgccggc cgcgtcgtcc cgggtccggc cgagaagacg ggcgcggagc
    19861 ttccggggca tcaggaaaag cgccgtgtgg ccgcccgaga cgatcaaggc gaggacgggg
    19921 aagggaagat ccggacggtc gaggaaggcg ctttcgatat gggcctggac gtgatcgatc
    19981 cccaggacgg gcttccggtg gtaataggcc aggcccttgg cgaaggaaag cccgacgagc
    20041 agcgagccga tcaggcccgg tccctgggtg acggcgaaga aatccatagc ctcgagcggc
    20101 aagccggcgg ccgtcaggct ctcccggacg acaggggtga tggatttcag atgttgacgg
    20161 gaggccagtt ccggaacgac gcctccgtac ggggcgtgaa cctcatcctg cgaaaggacg
    20221 atgttgctga ggatcgtccc gtcctccccg aaaacggagg cagaggtctc gtcgcacgag
    20281 gattcgatcg ccaggagatg ccgcatcgcc tttccggtca tttccggcgg agggcttcga
    20341 ggcttgcttc atagggcggg cgggccacgc cgttctcggt gacgatcccg gcgatgtagc
    20401 gggcgggagt gacgtcgaac gcgggattgc ggacccgggc cgtaggcggg acgatgagcc
    20461 gcccgttgat ctcccggacc tcggccgccg gccgctcctc gatggggatg aggctcccgt
    20521 cggccagcga aaaatcgaac gtgctgagcg gggcggcgac ataaaacgga aggttgttct
    20581 cccggcagag gacggccacg gagtatgtcc cgatcttgtt ggccgcgtcg ccgttgcgga
    20641 cgatgcggtc ggctccgacg acagccgccg agatttcccc tcgatgcatg agccagccgg
    20701 ccatgttgtc ggtgatgacc cggtagggga cgccgatccg ctccagctcc caggccgtca
    20761 ggcgcgcgcc ctggagaaac ggccgcgtct cgtcggcaaa gacggtgaat ttccggccgg
    20821 cttcccaggc cgcccggatg accccgacgg cggtgccgta gccggccgtc gccaggccgc
    20881 cggcgttgca gtgggtcagg accgcctcgc cgtccttgat cagcggggcg ccgtgggttc
    20941 cgatccggcg gcacagctcc tcgtcctcgc ggtcgatctc gaccgcttcg gctgcaagct
    21001 tcgcctggag cgaagccagc ggctcgccgc gatgggcttc gaagacggat ttcatccgct
    21061 ccagggccca gaaaagattg cgggcggtcg ggcgggtccg gtgaatccgg tcgtggacgc
    21121 gggcgaaggc cgcgtcccgg tcctcccctt tcttgaccgc ccgcatcccg atggcgaccc
    21181 ccatggccgc ggccactccg atggccggag cgccccggat gaccatggtt tcgatggcct
    21241 cggccgcctc ctcataagtg cggcattcga cgtaggtttc ctccagcggg agcttccgct
    21301 ggtcgatcat gatgatcctg tcgccggccc actcgatcgt cggacgcatg agtactcctt
    21361 gggaacgccc cccagtatag cgttttcctc cccccgggga aaccggaggg cggttggtca
    21421 gaacacggtc ttgacccgaa ccccgcgggc ccggagctcc cgggccaggg cccctccctg
    21481 gcggcggaaa aacgcgtccg ataaatattc gagcaggcca tgacgggcat agaaggcccg
    21541 gacggccggc tggtagttca tccggtcgat gaacacgtgc tcgacggccg gggccaggag
    21601 agaggccagc gtctcgggat ttccgggcag aagcggcccg gcgaaggcgt aggtccggac
    21661 gccggcggct ttgaattccc gcaggatctc gatccgctcg cggaccgggg cggcccgggg
    21721 ctcgaacagc cgggccgcgg cctcgtcctc ggtggcgacg gagaacccgg cttcgacgtc
    21781 cttgatccgc cggatgatgt cgaggtcgcg gcggaggagg gctgacttgg tttgaatcac
    21841 gacgggaaaa cccctcccgc cgaggatttc caggaggcgc cgggtcaagc cgaatttctt
    21901 ctcgagcggc tggtacggat cgcagaccga ggcgatccag atcgtccccc gtttggcctt
    21961 ggcgatttgc cgctccagaa gaagaggggc gttggtcttg acgtcgacga accctcccca
    22021 cggctcgacg tgcccgctgt accgccgcat gaacaaggcg gcgtaacagt agacgcaggc
    22081 gacctggcag ccggtgtagg gattgaggca gtagtcgtag atcttcgagg cgttcaggat
    22141 cgacttggct tcgatttcgc ggacgacggg aaccggagcg ggcgcctttt ctcgcatgtt
    22201 ctcccgctcc agcataacga ttctctctcc gccgcggcca tccttgatcc gcggccgggc
    22261 ggctgaccgc cggccctact tgacttccag gatggccagg aaggcttcct ggggaatgtt
    22321 cacccggccg acggtccgca tccgcttctt gccggccctc tgcttttcca ggaccttcat
    22381 cttccgcgaa tagtcgccgc cgtaaagctt ggccaggacg tccttgcgga aggccttgat
    22441 cgtctcccgg gcgatgaccc ggctgcccag ggcggcttgc agggcgacct cgtactgctg
    22501 gcgcgggatc gccttgcgca tccgctcgac cagggcccgg ccgaaggtgt acgctttgtc
    22561 ctcgtggacg atcaccgaaa gctcgtcgac ctgttcgcgg ttgatcagga tgtcgagctt
    22621 gaccaagggg ccctcccgcc agccggccag ctcgtaatcg agggaggcat atccctggga
    22681 ggccgatttg agctggttga agaaatcgaa gacgacctcg ttgagcggga gaaggtattg
    22741 gaggaggacg cgatggggac tgatgaattc gagcttcttc tgctcgcccc gccgcccctc
    22801 gagaagggcc aggatcgccc caaggtatcg gtcgggcgtg aggatcatgg cctcgatgac
    22861 cggttcggaa atggagacga tgtcctgggg ccggggcagc tgggacggcg tccggatgtc
    22921 gagggtttcg ccggaccgga ccttcacccg gtagctgacg ctgggagcgg tcgtcaggag
    22981 ggtcaggccg aactcccgtt ccaggcgttc ctggacgatc tcccgatgga gaagcccgag
    23041 gaatccgcac cggtaaccct ggcccagggc ttgcgaggac tccggctcgt aaaaaaacga
    23101 ggcatcgttg aggcgcagct tctcgagggc ctcgcggagg tcctcgacgc gggtctcggc
    23161 cgcgggaaag agcccgcaaa aaaccgtcgg cttcgcttcc ttgaatccgg acagggcggc
    23221 tgaggccggg cggtgcgtgt gggtgatcgt gtcgccgatc cgggcgtggc tcatcgtctt
    23281 gatgccggcg atcagatacc ccacttcgcc cggcgacagg gcctcccgct tcaccggatc
    23341 cggcgtgaga taaccgattt cctcaacttc gtaatcggct tgcaggccca tcagcatgat
    23401 cgcgtcgccg gtccggatcg ttccgtcgac gacccggacg aggacgacga cgccccggta
    23461 gttgtcgtac caggaatcaa acagcagggc cttgagcgga gcgtccggat cccctttcgg
    23521 cggaggaatc cgggcgacga ccgcttcgag gatctccttc acatttttgc cggtcttggc
    23581 gctggtgaga agagcttcgt cccggctcaa gccgacgata tgctcgagct ggtcgagggc
    23641 ggactccggg tcggccgaga cgaggtcgat tttattgatg accgggatca agtgaaggtc
    23701 gttttgcacg gccaggtagg tattcgccag cgtttgggct tccacgccct gggaggcgtc
    23761 gatgacgaga agggcgccct cgcaggcggc caggctgcga gagacctcat aggaaaaatc
    23821 gacgtgaccc ggggtgtcga tgaggttcaa gacgtacggc cggccgtcgg cggccgtgta
    23881 ttccagccgg gcggtccggg ccttgatggt gatcccccgc tcccgctcca gctccatggt
    23941 gtcgagcgtc tgggctcgca gatggcgggc ggcgaccgcc ccggtcactt cgagaaagcg
    24001 gtcggccagg gtggatttgc cgtggtcgac gtgggcgatg atggagaagt tgcgtatgga
    24061 gttcatggga aacattcatc ttcgcaaaaa aacggcgtta ggtcaatgag accggggatt
    24121 ccaagccgcc gggccttatg ttccgtcggg ggggaaggaa cccggagatt ccggctgttc
    24181 cggacgcggg ctcggccgcc ggaagcccct tattcgtccg cgaacggccg gtcgatcgcc
    24241 aaggcttcca tcccgcccag gcagcgggcg ccgtcctccg tggcgaccat gctgtcctcg
    24301 tgccggatgc cgaattcccc ggcgatatag attccgggct cgttgctgaa cgtcatcccc
    24361 ggctcgatcc ggagtttatt tccccggacc aggtagggat actcgtggcc ttccaggccg
    24421 atgccgtggc cgaggcggtg ggcgaaatac gcgtagtccg ggccgaaccc ggcgtcctcg
    24481 atgaccttgc gggcggccgc gtcgacgtcc tcgcaggcgg cccccggccg gcaagcccgg
    24541 aaggccgcgg tttgagcctt tttcacgatg tcccagatcc ggatcatttt atccgagggt
    24601 ttgccgaaga cgatcgtccg ggtgacatcg gagcgatatc cttccacgct gcagcctccg
    24661 tcgaccatga cggccatccc gtcggtcagt gtcctcggcg cccgcgagcc gtggggaaag
    24721 gacgtccccg ggccgaattg ggggccgccg cctccggtcg ctcccatcgc ggcgtgggct
    24781 tctccgatca gacccgccag gtcgcgggga gtcatgccct ccgacaaccg gccgaaggct
    24841 ttccggtagg cgagcttggt gatccgggcg gccagatcca tataagcgat ttccttggcc
    24901 gatttggttc cccggcagcc ctccgtcacc gatgccgcgt cggccggctc gatccaggaa
    24961 acgtctcgat tcaagccgta cacctggaag gcggcggtgt tcggagagag gccgaggcgc
    25021 cggccgccga ggtccttcag aattcctccg atcaaggcgt acgggctctc atgttccttc
    25081 caggtccgga tctcttgtcc ggagggaatc gtttccttgg cccggtccaa ttcgaaggcc
    25141 gggcaaaccc agatcggggc gcctttttta ttcagcatcg ctccgaaggt ccgctcgctc
    25201 gtccaccagc tcaccgaggt gaaataagcc aggttcgccc ccccggacaa gaaaacggca
    25261 tccagcccgc cgtcagccat ccgccgggcg gcgcgttccc gccgctcttc atagtccgcc
    25321 gggtcgagcg gccggacccc ggcggtaaga ttcccgacct cgtcgccggc ccccgccggg
    25381 gaggcgaaca atccgcggac gaaggccggg agggagagag tcaagccgcc gatctttaaa
    25441 aattctcgcc ggggaatcat cgggatctcc tggggaatgg aatgagcgtc cggatcatga
    25501 cggcgtatcg tctcaccggg agggatcgat gtcaaacgcg cgaccccgcc gggacggacg
    25561 aagacgcgtt tcattgactt ttcgagctct ctcgagtact ttcatagcgt cattcaagga
    25621 ggccggcatg tttctgttcg gcacggtcgg accgcaagag ttgctgatcg tcctgttgat
    25681 cgtcatcatc atcttcgggg cgaaaaagct gcccgatctc ggcaaatcgc tcggcgaaag
    25741 catccggaat ttcaagagct ccgtctcggg aaagggaaaa gaagccggga aggacgagtc
    25801 gaaagacaaa atcccgccgg ccgactgatc cgccttcccg atgctccgga cgatcgagat
    25861 gctggccgcg cggcggcgcg cggccgagga tgatttccgg cggacgctcg ccgagctccg
    25921 ccgcatatcc gaaagcctgg atccgttggt ccggcggctt tccgccccgg ccgaatcgtc
    25981 cggagagatc cggtccgccg tggccgaagc cgccgccgcc ttgaaagccc tggccgaggc
    26041 ggccgcccgg caggccgagg ccctggacgt cctttccgac gtccgcgaca agctgtggga
    26101 cgccgagggc aacaaccacg tcgggatgat tttcaagagc atggagtggc gggtcgaccg
    26161 cctcgcgatc gccgccgagg acgccgccgt ccttcttaaa acattcgcct ccctccagga
    26221 cgagctccat cggctgctgg ccgccgtcca agacaagaat ctgtccacgc ccgccttgat
    26281 cgccccgctg atcgaaccgc tcgaggaggg gcgctacctg cggttcgaaa accgcttccg
    26341 cggggccgag gaggaaatcc ggaagcagca gatcgcctat ctcgacgact ttccgccggg
    26401 cgggcggatt ctcgacctcg ggtgcgggcg gggggagttc ctcgagcttt tgaccgcccg
    26461 gggcttccaa gccgagggcg tcgacctcaa cgtcgagatg atcgcgctct gccgggacaa
    26521 gggcttgtcc gtcgagaaag gcgatcttct cgaatgcctg gccgcccggc ccgacgcctc
    26581 cctcgacggg atcttctcct cccaggtcat cgaacacctg ccgccggcct cgcttcgccg
    26641 gctggtcgag ctgtcctatg ccaagctccg cccggaggga cggattctcc tggaaaccgt
    26701 caaccccctg tccgtcttcg cccttgtcca catctatttc ctcgatttca cccaccggac
    26761 cccggtccac ccccaagccc ttcagttcct tctggaagcc gccggctttt cttccgcggc
    26821 ggtccggttc ggtctcgggc tggaggcgga aaagctcggc catattcccc ccgcggacga
    26881 ggccgcggcc gtcttcaatc gcaacgtcga tcgcctcaac gctctgttgt tcgctccgcc
    26941 gcagtattcc gtcctcggcc gcaagaccta agaccggcgg tcatccgggc ggaagcggtc
    27001 ttatttcaaa acacggcctc tttttcgcaa aagaaagcat caaaaacgcc cgttctctcg
    27061 tatcagagat ggcggaccgg agcgccggat ccgccggatt tgcattcaaa gaggatgttc
    27121 tttaaaattg ggctcttgca gaatcacgaa aggcgagatc cgatgacaaa acgaatcttt
    27181 gcgacaggaa tgctcttcct cgccctgggg gcggcggccg cggcggccgg tcccacggta
    27241 caggaaaccg ggacgatgtt cggccgggtc accgacctgg acggcaatcc gcttccggga
    27301 gcttccgtgt ccctcatggg cccgtctctg atgggcatga acagcagcgt cacctcggcc
    27361 accggccagt tcttctttcc tcttctcgcc ccgggcctgt acgaaatccg ggtcgagatg
    27421 cccggcttta agatgcagat ccagaggggg cttcgagtcc agtccggacg gacgatgcag
    27481 atccatgccc tcctggagga aacggccgtg gacgaggaag tcaccgtccc gccgggcgat
    27541 cagcgcgcgg acacgaggaa cgtcaaaagc tcctcagccc tgggctcgga caccctggcc
    27601 cggcttcccc tgccccgcga tcatttcctt ccgtggacct tggtccccgg ggcggtggcc
    27661 gatgtcgccc gggaccgccg tctcgtcgcc gttgagggcg ccgcgccccg gagccagatc
    27721 gtcctctggg aaggcgcgat gttgaacgac cccgtttcgg gactgccact gatccacccg
    27781 gtcgacgacg ccgtcgagga aatcgaatac gcgacgacgg gacttccggc ctcccttccg
    27841 gcgacggacg gggcctacct ccagatcgtc ggcaaagacg gcggcaacga ctttcacggc
    27901 gggctgtcct attattccac gggagcggcc ctggcccaag atctcgacgc gtcctccgcc
    27961 ggctcgctcc ggccggccac gcccgaccga tacgaaagct accgggatct gtccttccat
    28021 ctcagcggat cgttcatgga cgaccgggcc tggatgtttc tggccggccg aaggctgacc
    28081 tcgacggtct ccaatccgta ctctccggaa acgcggatgg ccgctcttgg attcaccgac
    28141 agcccggcct tcgacctggc ccgcaaggaa tggagcgggt acgccaagat ccatgtccgg
    28201 ccgacgaacg aggtctccta ttccggactc cttctcttca acaacttcac cgaaccctac
    28261 gacacggtct cggtttccgc cgggacttcc tccgaccgcg ttcccgagcg gagccccgag
    28321 aacgtcctcg tcaccaccca caatatccgt ttttccatcc gccagaacac ggcggctgag
    28381 ttccaagccc attacattca ccacggcttc accttggcct cccggggatc gaccggagcg
    28441 gcggccgttt atgacgcggt ccaggatgtg tggtggggcg ccccggcccg cgagggaacg
    28501 gaaaaaagcg aggcgttcgg cgtttccgcc gccttgacgc atttcaagga agacgtcttc
    28561 ggggcggatc acgacctgaa aatcggattt tcctacgatc aagccgagac gcataacgac
    28621 tggtatcggg ccaatccgtt caatacgtat tggttcgatt acaagacgaa aaacccttat
    28681 tattacgacg gcctgtcgct cggccgcctg gaagttcttc cggcgccggc ggccgccgcg
    28741 tcctgggacg tctcggagat gacccgcaag ctcggagctt atgtccagga cacgctgacc
    28801 cggaaacgcc tggccctcaa cgtcgggatc cgggctgact ttcagattct ctcccttccc
    28861 cttcaagggc ggaacctgac gagcccggcc tatgctcctg cgtttttcaa caccgccctg
    28921 gatttgaagg atttcatgaa ggcgatcgac ggcctcatcg acgaggccgg ggtgacatct
    28981 ccgctctcct ccatcaccac cctggagcgc acctcggttt catttttcac cctctccccg
    29041 cgggccggtc tcatcgtcga cctgctcggg aacggccggg ccgccctgaa attctctttc
    29101 gcccgctctc atgagccctt gtggatctcg ggctacgacg aggaccagtt cctcgagccg
    29161 cagacgctca ccttgatctg gcacgatttc aactccaacc ggctgatgga tatgccgggc
    29221 acggatgact acacccttct gtcctggcgc ctgcagaatc tcgattccgt ttattacgac
    29281 ggcgtcaagg ctccccgaac cgacgaattc acggccgggc tggagtacgg tcccgcctcg
    29341 aacctgcggt tggggctcaa gctcacctat cgtaaaacga cccgcctcgt cgaaaacatc
    29401 gattcggtca acggatccga tccgctggcg acggatgcga tcgggcggat ctggctgccg
    29461 atgacggtca ccgatccggg cgccgacgcc ctattcggca cggccgacga cgcctccctc
    29521 accgtttacg gcctccgggc cgaccggccg gctcccgtct gggtcgcggc caatcccgag
    29581 gacgcctacc ggaaatacct gggggcgacg ctgacgctgg aaaagaggat ggccgacaac
    29641 tggcagctgc agggatcctt caccctcagt tcgcttcggg gaacggccga ttacgccctt
    29701 cccggccgga tcaaccggac gtttctattc aacgatccca acgccctgat caacaccgag
    29761 gggccgctgg cctttgaccg gcccgtccag ttccggctct cgggaaccta cctgttcccc
    29821 ttcggcctta ccttcagcgc cttctttcag tactattccg gcgccccgtg ggcccggacc
    29881 ctgacggtgt atttccccgc cggttacatg ggttatggaa cgcgggaacc ctcggtcacg
    29941 gtgtacgccg agccctgggg gacaaaccgg gcgccgggcg tcgcctgcct cgatctccac
    30001 ctggagaaac gattcaccct gaaaaagggc gcctcgctga ccctgatggt ggacgtcttc
    30061 aacacgaccg gccggaacac gcagacgatt tcccaggacc gagccggcat cctcgacgaa
    30121 cgcaagacgc cggcgcgtta cacggtcacg caggacgagc ggatggtgaa tctttacgga
    30181 gtccgccagt tccgggtggg gatcaagttc gggatttaaa gccggtcatt tccactcgac
    30241 ggatttctcg acgtcctctg atcccgtttg gttggtcagc ttcacctcga ccgtcggcgt
    30301 cccggcggcg agacgggcca gggcgtcctc ctggatgacg tagatcggga tctcgatgac
    30361 atgaacatcc ccgtcccgct tgtggatttc cgtttcgtcg taattcgcct cggccgagaa
    30421 ggttttctcc caaaccgtct ttttctcctc atcccggacc cggacgaaaa ccgtaaaggt
    30481 cgtgaggaat cttcccccct cggatttgaa ccagatcagc cggaagggga tttcgagccg
    30541 gagcatgcct tccatccggt ccgcccggcg gaccgtggac cggagctccg cggaaaaatc
    30601 gaggaccgcc cgggctccgc gggtacggct gaccggctgg gccggacgtc ttcccggttc
    30661 ccccggccgg ctgccgtccg gcccgagcgg cgcgggcggc tggttgaggc ttaattcctt
    30721 gagcggcgcc aggtcgaacg tcacttgacg gaaattcccc gtgcacattt cgtcgagaaa
    30781 ctggatcggg aatcccttgt agtaccagat ttccaggcag tttctccccg aggtctcggc
    30841 aagcatcgga accctcttct catcgggagg gccgtagagg acataaatcc ggccccggtc
    30901 cgtcagccag cccttgacgg cgtccctccg gaacatttca ttggcctggg cgatccgggc
    30961 gtaatactcg gaccggactt cgttgtcgga cgtatcggga tccggatcgc gccgggccca
    31021 gaaatcctcg atgaacgcgt ccttcccgga atcgggcgtg gacaggaacg ttttcctctc
    31081 ggcattagtg atcaagtagc ggacttcgct gagaaactcg gcgtatttct ccggcagctc
    31141 ccgctccatc tggtaaagcg cgcagccggc cgatccgagg acggtcaggg ccagggcggc
    31201 ccacgcggcg atcgccggga ggacggggac ttttttcatg atcgactcct gtccggaatc
    31261 ttattttgaa cgtccgggaa atgcaaatcg ttccaccaac gggcgggatt cagcctttgc
    31321 agatctccga cgcccggcga tagtatttgg cgatatgctt ctcctggtag ccgtaggacc
    31381 agtggacgtc ccggatgtgg gcggccgcct cgtctttttt cccggctttc accagctcga
    31441 gaaaggcttc gtgctcgccg atcgaggcca cttcccattc cttgacaaat ccctgctggc
    31501 ggggaaaatc gtacagccgc cgcttcaggt tgttgacgac cttcagaagg ctggcgttgc
    31561 cgcaccggga gagaaagaca tcgtgaaagg cgaggttctt ctcataatag aggtcgaagt
    31621 cgtcccgccc gatcgcgtcc ctcatctccg cgttgagacg agtcatctcc tcaaactcgg
    31681 gccccccgag gagagcaaac gcctggcgga gggccgtgct ctccagcgcc ccgatgatct
    31741 cgtagtaatt cttgatgtcc tccgggcgga ggacgttgac cacgactttc cgccgcggca
    31801 aaatggtcac aaatccctcc atctccagct gaagaagagc gtcgcggaga ggggttttgc
    31861 ttacgcccag gcggagggag gtctcctcca tatcgatgac cgagccggga aggatttcgc
    31921 cccggcgcat cgcctcccgc aaaaactcat agacctgttc cttcaacgat ttgaggttga
    31981 gcaacggttt cgtcttcacg cggtctttcc ctcaggcagg atatttttcg cgcctccggc
    32041 gccgctcaac tataggactc cggctgtttg aagtcaatgg gctccgcctc cggccgggga
    32101 ggcggcgagc gcctccagct ttttccgaag ctccggctgg ccgggattca gggccagcga
    32161 ccgctcccaa gcggcccggg cttcggctcg ccggttcagg ccgagataac cgtctccgat
    32221 gaggttcaga agccggggat gaatcccgaa ccgcgtcatc gccccgtcca ggacgccgac
    32281 cgcgcctccg aaatccccgc cccgcagccg cgtctcggcg gccaggatcc agacgtcgta
    32341 caccggctcc gcccccgccg caagataggg atcgaggatg aaagcggcgg ccgcgtattc
    32401 gccggcccga tcataaaccc gggcgagggc caaggcgatt tccggcccgg ccgtcctccc
    32461 ggccacggct ttttccaaga acggccgcgc ctcggccgcc cggccggcgg cctcgagctg
    32521 ccgccccagc agcccgtcgt acgccggatc gccgtcggcc ggcatccgct tggcgtgaat
    32581 ccacggccgg gggaccgctt ccagcggcgt gacggcgaaa tccccgcccc cggcggccgc
    32641 ttcccgccca tcgatgacaa gccggacgcg ggcggtgtag aaagccgggt tcaatccgga
    32701 cgccgggatc gattcaacga cgtccggaac gccggaaaga tcccgctccc attcctcaac
    32761 cacaacgtcg tcccgggtga tggaaaaccg aaggcgggcc cggcttcggg ccgggccgtc
    32821 gaggccgaga acttggaagg ccaggaccac gttgtccgaa cggacgaggg tcgggccgga
    32881 ctgaaaataa acctgggtcg agccgaacaa gaacggtttg agggctgagg gttccggccc
    32941 ggacggccgg accctatgcc cgagaagcgg cgcggtgagg ccgggcccgg agccggattc
    33001 ggggacgacg accgtccgct cgaacgcgca aaattccttg gacgcctcgt tcttgagcag
    33061 cagggaaact ttgaacgttc ccgggatcaa ggggaagaga tcctggatca tgaacggcac
    33121 gcggctcagc gtgtttttcc gctcttcgtc catccggacg gaaacgattt tttcgaaggc
    33181 ataaatgatc cggccgtcct ccagcgaaac ggcgccgttg aggcggagcg tcgcggcgta
    33241 ttcccggccg gcggcggcga gggaaagacg ggccggctcg atggcgtaat ggacgaacgc
    33301 ggttccggaa ggctctcgcc ggacgatcat cgtcccccgg ctgtccaagt aattggcgct
    33361 gtattcgacc tcgatgatgt ccttgtattc aaggaacttg gccgcgtact cgtcgtcgat
    33421 ccggccggcg ccggaagccg cgattttttg gagaagaatg tcggaggaag gagacgggcg
    33481 ccccggaaaa ccggacgcct cgccgggaat cagggaaacg gaaaccgcgg ccaagcccgc
    33541 gtcgagagcg gacaaggccc ggcgggcctt ctcataatcc ccgggatccc cccgaaaatc
    33601 ggagaggagg gccgccggtc cgtcctggaa cgggctgtag atcttgaagt cgccgacgga
    33661 cccttcttga taaaaaacca gattgaacga cggcggcaag ccggccgttc gcgcgttctc
    33721 ctcgttccgg tagaaccaga cttcggcctc gcggattccg tccttgccgg aataccggac
    33781 gatgtcgtcc ggctcgccga ggaggacgta catgcgcccg cggccggttt tccggccggg
    33841 ccgggccgcc gtgcggccga aatgcgcgtc ggcgtaagcg agccggcgct catgctccgc
    33901 cttgaattcg ttttccgggg tcgccggaac gggatcgcgg cgcttccaga aggcggcgat
    33961 gaaaagatcg cgctcgcggt cggagtcgag ccggaggaaa acgtcccgct cgcgggagga
    34021 aaggatgccg tcgacgtcgt cctcgagcca tttccggtgg acgggggaca gatctgttcc
    34081 agacgggcgg gcgggcccgg ccgcgccgag aaaaagacag gccgccagca tcaagatcgc
    34141 cgggcctacg gccgcggagc gtcgcttcat gtttttctcg atccggcttt cattttacaa
    34201 caatcacggg gaggcttcac gccggccgtc tccgaatggg atcgaggggt tgagcgaaac
    34261 cttccctttt tctatcatac ccggaggagg gatctcatga tcgagctgga aaacgtttcg
    34321 aaaagctaca acaacggccg ggtgaaggcc gtcgacgacc tcaccctgac cgtgcggccg
    34381 ggggagatct tcggcttcct cgggccgaac ggagccggca agaccacgac catcaagctg
    34441 atcgtcggcc tcctccggcc agacgccgga accatccgga tcgacgggat cgacatccgg
    34501 aaggatccga tcgcggccaa atccgtgacg acgtacgtcc cggacacgcc ggccgtttac
    34561 gagcgcctga cgggcctgga gtacctgaac ttcatcggcg acgtctacgg cgtgccgaaa
    34621 gccgaacgcc tccggcgcat cgagcactgg cttgagattt tcgacctctc cgcggccgtc
    34681 gccaatccaa tccaaagctt ctcccacgga atgaagcaga agatcgtcct gatcgccgcc
    34741 cttctgccgg cgccgcgggt gatggtcctg gacgagccga tggtcggttt ggacgctcgc
    34801 tcggcccacc acatgaagga gatgatgcgg acccattgcg ccggcggcgg cacgctcttt
    34861 ttctcgactc acatcatgga agtcgccgag aagctctgcg accggatcgg catcatcgat
    34921 cgcggacggc tgatcgccca tgggacgctc gaggagcttc gggcgatcga ccgagaaggc
    34981 cagtccctgg aaaagatttt tctggagctc accgcggaat gaaaaaattt ttccgtctcg
    35041 tccgggtcgg cctccgggtc aatttcggcc tgtccctcct ccggccgaaa gcccttctcg
    35101 aaaacaaacg cgacttctgg ctgatcgttc tgatcctcgt ggggatcgcc gggttgagcc
    35161 ccgttctgct ttattatctc aagggcatcc gcttcctcta cggcctcctc gctcccctcg
    35221 gccagcaggc ggccctcctc acgcttgcga tcctcgcggg ccaattcctg atcctcgtct
    35281 tcggcttcta ctacgtcctc tcggcgtttt atttctcccg ggacttggat ctccttgtcc
    35341 ccctgcccgt caccccggcc caagtcctcc tttccaagtt ctccgtcgtc gtcgtgaacg
    35401 aatacttgac ggcctcgctc ctcgtcgtcc ccgtgctggc cgcctacggc tttcattccc
    35461 gggccggcct ccgctattgg gccggcgcgg ctctcgtcta ccttttcctc cccgtcattc
    35521 ccctggccgt cgtttccctc ctcgtcgtcg ggatgatgcg tttcgtcaat gtcgggagga
    35581 aaaaagacgc cctgatgatc gccggaagct tggtcctgat ggccgcggcc ctcggcctgc
    35641 agttctggct gcggagcctg cccgcctccg gcccgggccg gcaggcgctg gtcgcctttt
    35701 tctccgaggg ggggatcatc cgcaaaatcg gagcgtcgtt tccgccgagc gtctgggcga
    35761 cgcgggcgct tgtcccggaa gccggagcat ccgggctcgg ctcattcctg ctttatgcgg
    35821 gagtctcggt cgggctgttc gcggccctct tcctcgccgc ccgggcgctt ttctaccggg
    35881 gactcctcgg cctcggcgag atctccgccc gaaaacggcg cctctcgcgg gaagctcttt
    35941 cggcccgggt gtcctcgggc cgccggccgg tgcgggcgat cttcctccgg gagttgcgga
    36001 tcatgaaccg gacgccgatt ttccttctca acggcgttct ctcggtcgtc ctcatcccgg
    36061 tcat
//
LOCUS       DAOOXH010000016        33350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_1107_length_33350_cov_7.458627, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000016 DAOOXH010000000
VERSION     DAOOXH010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 33350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33350
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1107_length_33350_cov_7.458627"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PKV64_03480"
     CDS             complement(<1..919)
                     /locus_tag="PKV64_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16176.1"
                     /translation="MNPEKESADKAARKQYFTKVKEDFPDVLAFGEQKFHKRMSMRYG
                     ENPGYPAAFYAEENAFGANMATLEVLQEGSKGLGYINVGDMDLGQRLARKLTAVFPGQ
                     AAVVLIKHEIPSGVALGVDALETFEKAWNADPLSNFGSVDVFNVPVTPAVARLLVESP
                     RNIEVVYAPGYVENSLEILASRKPLRVVRMGGPIDGPAGDNGLELKRVAGGLLVQKRF
                     DSRIVSPADVDVVSRRKPSAEEIQAALLAWNVAAFTRSNAVVIGTKDRIHGIGSGQRS
                     RIDSTEDAIRISRRGYGPAGCVLASDAFMP"
     gene            complement(916..1653)
                     /locus_tag="PKV64_03485"
     CDS             complement(916..1653)
                     /locus_tag="PKV64_03485"
                     /inference="COORDINATES: protein motif:HMM:NF019443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HPN16177.1"
                     /translation="MTTHPLEALRRMEYPGRVVVLGRDPAGLHDVIVYAVTGRSPSSQ
                     ARRLEREGNAVVTKPTDPEILKTGQPDLLVYPAVLFGGRPAVSNGRQTDDVARTEADH
                     PVAALAAALRNWSYEPDPPIYTPRISGLLWPDGRAALSLIKRGFDGSARRLFFEFSLH
                     PGRAEMIATYAGPNRNPLPVFEGEPLSLTLVETTAKATAGVFYEALRPEGRSDDFRVA
                     AACLFLSRNEPGKPEIALINRHERTGA"
     gene            1785..2549
                     /locus_tag="PKV64_03490"
     CDS             1785..2549
                     /locus_tag="PKV64_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPN16178.1"
                     /translation="MTKNCVIPTIAFKNDVFLHSPEARSLRILSEYLEPDMRFDRNKI
                     NSTVLFLGSARVHADDASAPLHPYYRDAEELAFRLARWAIPLRPTGKNFVVCTGGGPG
                     IMEAANRGAARAGGKSIGMNISLPHEQSPNPYIPEDLCFLFHYFFMRKFWLVSRARAV
                     VAFPGGYGTLDELFETLTLIQTEKIAREEVVVLLYGEEFWKEVVHFDALVKYKAIAPS
                     DMKIFHVASTPEKAFAFLKKKLLRFCSVYGPPRFQG"
     gene            complement(2522..2908)
                     /locus_tag="PKV64_03495"
     CDS             complement(2522..2908)
                     /locus_tag="PKV64_03495"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPN16179.1"
                     /translation="MRKIPAFLPAALYYGLIYVLSDRPIRGIPRLFPLQDKIFHFILY
                     AVFGLLLIQGLNRVRVEKAVRFAALIALGIAAAGLDEFHQSFVPGRTADWADAAADIL
                     GVLAAWGGVRLYLRFRPSYPWKRGGP"
     gene            3128..3787
                     /locus_tag="PKV64_03500"
     CDS             3128..3787
                     /locus_tag="PKV64_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HPN16180.1"
                     /translation="MKRAVSDRRPGPGVPRSAAVLLLSLLLSAPACAGGRMIAGAPGQ
                     TGIASWYGPDFHGRLTSNREVYDMYDMTAAHPTLPFGTRVMVTNLDNGRAAEVRINDR
                     GPFVKERIIDLSYAAARMLGMIGPGTAPVRLDILNRKDWPPTVGMVIQVGSFTNEQNA
                     RRLQAELGRRFGETFISTVVCSGRVFYRVRIKVDDRSGADLLAGRLSASGYPVLICEA
                     D"
     gene            3905..4597
                     /locus_tag="PKV64_03505"
     CDS             3905..4597
                     /locus_tag="PKV64_03505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HPN16181.1"
                     /translation="MTDIRGIFAEVAPTYERANRVLTLGLDRRWRRAAARTAAARGGR
                     LWLDVCSGTGDMAEELAGRIPAGGRVIALDFSGPMMAGLKSKPRFARRGAAVRAEASF
                     LPFPAGTFDLVTVSFAVRNLNRGRESLRRTFAELRRVLKPSGRLVLVETSRPPVLPVR
                     WLYFFLARALIPRIGEKISGSKAGYAYLGATIPRFYGAPELAVILDQAGFARVRFRRL
                     CLGAAAVHVASG"
     gene            complement(4594..5889)
                     /locus_tag="PKV64_03510"
     CDS             complement(4594..5889)
                     /locus_tag="PKV64_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16182.1"
                     /translation="MRLIHKTFFLAAAVAVLFPHVFPETVFSQEIPVKPDAGRPEKLT
                     VFLSGPEAAAYASEIPFAAVAAGPGSARVVVDIVRSGDAQGEIIVLEFSGREDFAGRT
                     DKLVYRPRPGETAETTRKETARLLQLGLLRFASHTPAAGRLDVLFEDAVKPTAVDDPW
                     NFWVFSLSANGFLNGQKSSRFQSWFGALAANRVTPEWKIRASIDFSRDKNSYDYEGYL
                     YENVSTSKNASGLVVRSLNDHWSLGLSVTAGSSTFNNYDFRLAVRPAVEFDLFPYSEA
                     TKKQLCLLYYAGPEFVRYREETIFDKRKETLWKQTLSIALGLKRPWGSMSAVLTGSHY
                     FHDLGKNRLRFDADVSWRIVSGLSLTFDFGGARIRDLLALPKGGASLEEILLRRRQLA
                     TGYDYYLSAGLSFTFGSVNSNLVNPRFGDSGGTSISISF"
     gene            complement(5901..7205)
                     /locus_tag="PKV64_03515"
     CDS             complement(5901..7205)
                     /locus_tag="PKV64_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16183.1"
                     /translation="MKKIPKPAVFVLMGCLFAALPGAAQAPPDDLEALKKTAPRIFID
                     CGSCDLEYIKDEIPFVNYVRDRKEADVHVLITTQTTASEGKEYTLTFMGRDGFSGIDD
                     SIRYYSKKDDTEDDVRKGLVRTLKQGLAAYVVRTPIASRLQVRYSPENGPAAGPDRWN
                     HWLFNISVDGYLEGEESASKGSIGLNATASRVTEEWKLRLGFSSSFRRDSFVYQGTKI
                     ESSQESYGASGLLVKSLGPHWSAGLALDADSSSYGNIAFRLVPAPAIEYNLFSYAESS
                     RRQLRFLYRLRFENVRYREPTIYDKTRDRLWGESLSVTLDLKEKWGFISSSLSGAHYF
                     HDFKKNNLTLNAFLQLNLVKGLSAYLFASGSRVHDQLALARGTATLEEILLERRELAT
                     SYSYFAIFGLSFTFGSAYTNVVNPRFGQLGGGDSGGVTIRVN"
     gene            complement(7378..9744)
                     /locus_tag="PKV64_03520"
     CDS             complement(7378..9744)
                     /locus_tag="PKV64_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HPN16184.1"
                     /translation="MIRAAVLLAGIGAAVLAFRTPAAPEIAHPWKVAYIGSLDAAGWC
                     GLVLAPHKDAAFAFRFQVEKDGRWIDGDDFFWLASEIGAQSPDGLYARIGFDLALPFL
                     PDRKEDTPILIKPSRRADLLVLEWSRQDEGAVVGRLMFPKNVPVRLVPYFPWHFQGRC
                     RISTDAELVAESHEGKPSTWVVWTDPPGAPAGEAEGRGEVLSFPAGGKRAVYFAAGVG
                     DGVENVRRRLYRYKNAKTIDVLIEEERGRYEKKRVAVEGLYGGAAEAVTNNLHWMMLY
                     QAGGHRLYASAGRQDASGRSPEDSDPWRISAGDSLFNALELSLESSRYAADAVRAALE
                     TQYPDGNMPQWRSRFGGTPDRSQPPVGSFCLLKLFGKTGDRELLEFAYPHLKKWHAFW
                     LEPGPGGKPRRDGNGDGLLEWGSDRKLVSAEAAPAEIRAKRESGQDDLPNWDDVPVSP
                     ASGTFMLNAVDLNSLYALDAWCLSQIADILDDRAGHEAYLKEYESVRKLINDHLWNSR
                     EGFYFDRHWDGRFSRRKAASNFFPLLARIPDESRAQIIRKHLLNPKEFWGDFVAPTIS
                     RDDPAFNDPDNPSRQHWRGTIGPAINYLIYQGLKAYGFDAEASEFARKGFVMFFNAWK
                     NYQLSPENFHPLSGEAAGRRHHGFGPLLALLAVEEYLDFTPWEGFRFGLISPEEEGLI
                     SRVAIQGRHYDVSVSRKEIILQEEGRPIITIDGGAVVRHFLYAENEVSFEIQSMAGRR
                     LRIEFLRKGKYQLLIDNIPKRVFTGRRIKFDVPDGSHAVLLQLLEALD"
     gene            complement(10113..10739)
                     /locus_tag="PKV64_03525"
     CDS             complement(10113..10739)
                     /locus_tag="PKV64_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16185.1"
                     /translation="MKQRWILPAAFGLAWILGGIPAEAQSPRPTFALQAGGAGHEENN
                     LHVGLASGFGFAFPLTLRLAATVEIDHWSTESRNSFGKLRQGRLAVTPVLVGLRYEFR
                     GNGYFVPYVAAGAGYVGTRFRIGSRVLSPPDTTVRQNVRSGGAGFLGLGASWRLSPLW
                     EFFSEIDYLIRTAPGRTITRHPGGALSQADLWVNLHVVYWKFGLRFLF"
     gene            10843..11973
                     /locus_tag="PKV64_03530"
     CDS             10843..11973
                     /locus_tag="PKV64_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPN16186.1"
                     /translation="MNLPIYFRSRQGEMAAFLKELVLAESPTGDKKAVDACSSLVLSR
                     CARIGVKHTRYPLRDRGDFHLLEYPSKADDRLEGRLLLLTHIDTVWPVGRLARMPFYL
                     QGGKVFGPGVLDMKAGLVMSYFALRALHELNLKPRRRIALFINSSEEARCPEADEVIR
                     TQAKASDRVLCLEPALPGGALKMQRKGRCVVRLDVSGVAAHGGNPAKGVSAIDELMTA
                     LRKIQMLRSADISVNVGLIGGGEKANVVAEAAFAVCDIRFWTNDQKARILRFVKALGS
                     GSRRAKIRVTVEGTTPPMEKTRISETLFREAQAVASEMGVRLSAGKSSGGSDGSIAAG
                     AGRAVLDGLGPDGDGIHAAHEHLLLPSLIERTALLTAFLQKL"
     gene            12112..13257
                     /locus_tag="PKV64_03535"
     CDS             12112..13257
                     /locus_tag="PKV64_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HPN16187.1"
                     /translation="MIKKYYLLSPGPTPVPSSVLSAAAEPIIHHRTAEFSKIFMEVTE
                     GLKYVFGTKEDVYVLTSSGTGAMEAAVANLCSPGDQVLTINAGKFGERWGNICKAYGV
                     INKDIVLKWGTVLPKEALEAELKANPAVKAVFATLSETSAGQIFDIQGFGEVVAKTPA
                     VLVVDGISGLGATPCPMDEWNVDLLLTGAQKSFMIPPGLAYLACSPKAWKLVETAKCQ
                     KFYFDLKKARKNLEKQTTPWTPGVTLIIQQKKALEIIREMGLEKLYVHHRVLGDATRA
                     GIKALGLELLAERPGNILTAVKTPAGIDGVKLVKTMQNKYMAYIAGAQDPHKGEFFRI
                     AHLGYMGGFDIITALTALEMTLTDLGYPVVAGASIKAAEPILRENWA"
     gene            13254..14153
                     /locus_tag="PKV64_03540"
     CDS             13254..14153
                     /locus_tag="PKV64_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPN16188.1"
                     /translation="MKKILIADALDKESMELLKAVPGFDVVVKTGLDEAGLIQTIPGF
                     NAVVVRSASKITRPVIEASSGLQIVVRAGIGLDNVDSAAAKEKNIKVANTPSATTITV
                     AEYAFCLMLGAVRQVGRANVTMKQHKWEKKTLHGTELYGKTLGLIGAGRIGLALAERA
                     IAFGMRVLAYDVIPIQTKLPVAQVPLDELLAQADVFSLHVPKTKQPILGPAEFAKMKD
                     GVVIVNAARGGVIDETALLTALQSGKVRAAALDVFAKEPPEDWTLIDHPNVTACPHIG
                     AQAEEGQKRAGLEVIRLLKETLA"
     gene            14317..15474
                     /locus_tag="PKV64_03545"
     CDS             14317..15474
                     /locus_tag="PKV64_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPN16189.1"
                     /translation="MVYLDNNATTPLDPAVIEKMAWFMGGHFGNPSSLYPIGREVKNL
                     INEARAHVAAAVGAAKASIYFTGSGTESDNQAILGVIDALPDKREFVTTTIEHPAVSQ
                     TAAYLEGKGYKVTYVPVDATGTIDLDALRSAVTPQTALVSVMHANNEIGTIQPIEDVV
                     RIAREQGALVHTDAVQSFGKIPFRADDLGVDLLTVSAHKIYGPKGAGALYVRKGTPLR
                     PFIHGGHQERGLRAGTENTIGIIGFGEAARILPLHFEADSARLRRLAGRLKDGLEAGV
                     PKIHFNGHPVNRIPTTLSYAFLGLESEAILLSLAAQGIYVSTGSACSEESEDVSPVLR
                     AIGLRPEFARSTVRMSLGRFNTDDDVTEILRVLPEVVDKLRKISPWDPEEF"
     gene            15492..16376
                     /locus_tag="PKV64_03550"
     CDS             15492..16376
                     /locus_tag="PKV64_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPN16190.1"
                     /translation="MSASPLFNITGRPILLDGALGTELMKVLPAPGLCPELANLEHPE
                     AVRSVHRAYFEAGADAVTSNSLGANPLKLAAAGLSGRARELAAAAVRIAVSVRPAGKF
                     VAGDIGPTGKFLKPQGPGTEMEFEDGFAVQAEGLAEGGADFLLIETMYDLREALCAVR
                     GARRGAPGLPVLATMTFNRTKRGFFTLMGDGLEKCARAFEAEDLPAFGANCTLPSGEM
                     ADLMAAWKPLTDTPLIAQANAGKPEIVDGRVVYSQTEDEYLAEIPRLIENGARIIGGC
                     CGTTPSFIRRMAVLIGAV"
     gene            complement(16360..16899)
                     /locus_tag="PKV64_03555"
     CDS             complement(16360..16899)
                     /locus_tag="PKV64_03555"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HPN16191.1"
                     /translation="MAEIRPFAPVKLVCGIITGREERAAEAERRLGERFGPVEARGPR
                     YPFAGTAYYQEEMGPGLSRGFISFRDLIAPERLPDIKIETNALEEELRKHFATPGRAV
                     NLDPGILTAAALFMATTKEFSHRPPLRNGIYAHLELLFVRNGVKRLEWTYPDLRTEDH
                     IPFFLDVRKTYLETLKPPR"
     gene            complement(16892..17140)
                     /locus_tag="PKV64_03560"
     CDS             complement(16892..17140)
                     /locus_tag="PKV64_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16192.1"
                     /translation="MTELISTEANIARMRPFSMPAGETPAARPRENGRKPPWLNFPVN
                     AIGGIGLQDTKNGGQTEARARGGRLETPVDFAYRKGDG"
     gene            17186..17800
                     /locus_tag="PKV64_03565"
     CDS             17186..17800
                     /locus_tag="PKV64_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPN16193.1"
                     /translation="MSQASKWNETLETIAARRSIRRFDGREVPRETLEIILNAANRAP
                     SAHNRQSWRFLVVRGEKKEELARLAASRAAGFPRPASVLLRMAARSISGAPVVVAVAN
                     TGELIAHGWSLFQGKDESTRDFFRIMEIQSSAAAVENLLLAASSLGLAAVWLGILYLI
                     KDDVRRLLGEPRGEFVAVIPVGRPAVETPSPAKRPLAAVVRFLE"
     gene            17866..19158
                     /gene="asnS"
                     /locus_tag="PKV64_03570"
     CDS             17866..19158
                     /gene="asnS"
                     /locus_tag="PKV64_03570"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPN16194.1"
                     /translation="MKWVYIEDLASARDQDVEIRGWVYNTRSSGRIRFLLVRDGTGIV
                     QATIFSREENDPLFEKFDALTQESSVIVRGRVREDKRAPGGVEIGVSGIEIIQIAKDY
                     PITPKEHSTPFLMEHRHLWLRSRKQHAVLQVRAEVIRAIRGFFDDRGFRLMDTPILTP
                     SACEGTTTLFETKYFDQSAYLSQSGQLYNEATAAAFGKVYCFGPAFRAEKSKTRRHLI
                     EFWMVEPEVAFATLEDTIALGQDLVLFILERVLTRRRADLAELERDAAPLEAIAAPFP
                     RLTYDEVFPRLQEKGSEIKYGDDFGAPEETILSDLYAAPVCITHFPARIKAFYMQPDE
                     QRPDLALGVDFFAPEGYGEIIGGGQRIHDPDLLAERIREHGLPPEPYQWYIDLRKYGT
                     FPHSGFGLGVERVVAWLCGIKHIRETIPFPRLLYRIYP"
     gene            19179..20480
                     /gene="rimO"
                     /locus_tag="PKV64_03575"
     CDS             19179..20480
                     /gene="rimO"
                     /locus_tag="PKV64_03575"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPN16195.1"
                     /translation="MVTFGCAKNLVDSEVMAGHLRSAGYVLVPDTARADVIILNTCGF
                     IRPAREEAEKGIRRALKIKASRPGVRLVVTGCAVENDRPGLLERHPAVDAWLGVRDYD
                     RIVAAAENRPFRSRARTFLYDHRSPRLLSTPPGWAYLKVSEGCSHQCAFCSIPSIKGP
                     YRSRTVSSIIAEARFLEARGAREIVLVSQDTTAFGRDRGRADGLALLLAELLKGTAVP
                     WIRFLYGYPEEITDALLEVMADSRICPYLDIPFQHASPAVLRRMKRASSAARILRLLE
                     KIRKRLPEAAIRTSLIVGFPGEGPDEFKRLQEFVREARIDHLGVFTYCREEGTAAWAL
                     GDPVPEAEKERRRGILMEAQAAYSAENLRKFRGRTLDVLIEGPRPGRPGGWIGRTKFQ
                     APEEDGVVFVRAEGARTWKSPLGRVKIISSGTYDLFGNIAS"
     gene            20477..20926
                     /locus_tag="PKV64_03580"
     CDS             20477..20926
                     /locus_tag="PKV64_03580"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HPN16196.1"
                     /translation="MKSVVRLASTFFGAGYFPVAPGTFASLEAALIYGFAARRLAPLP
                     YLLLLAAVFLVGTAAADSFARTLNEKDPRPVVIDEIVGQGAALFLCPAGWPWIAGGFF
                     LFRVFDVLKPWPIRRLERLPGGWGIMADDVLAGVFTALVLQGIRGIL"
     gene            20923..22173
                     /locus_tag="PKV64_03585"
     CDS             20923..22173
                     /locus_tag="PKV64_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HPN16197.1"
                     /translation="MKRVVIVAVGSEMLTPFFQDTDSLFLTRRLNDFGLDVEEKIIVG
                     DESDSLKKAIREGRDRADLLFVTGGLGPTDDDRTREAVAAVLNKPLDVDGRVLAAIEE
                     RFFRRGKTMPPANRKQAEVIRGADVLANPAGTAPGLWIEEEEKIIVLLPGPPRELESV
                     FEAHVAPRISGRSRAHLARKVLKAAGLTESEVETMIQGLYPRDPGRRLTILAFPGQIE
                     LHVSAFSDVSAEEAERTAESLASALRERLGPAVFSDDGSDLEAVVGRLLAERKETIAV
                     AESCTGGLVCRRLTRIAGSSAYFLEGLVTYSNQAKIGRLGVPARMIEEHGAVSGETAR
                     AMAEGVRRVSGADYGLAVTGIAGPSGAAAGKPVGLVFCAASGPGGTRVERFLFLGDRS
                     HVQAQAAQGILDILRRRLEEKPAR"
     gene            22170..22733
                     /gene="thpR"
                     /locus_tag="PKV64_03590"
     CDS             22170..22733
                     /gene="thpR"
                     /locus_tag="PKV64_03590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPN16198.1"
                     /translation="MRTFIAVDPPEEVKARIHEFVRPFRQSFSRGIAWSSSRDLHVTL
                     KFLGEIEESRIPLVKEAVRSAAAAAAAFPVAVAGTGSFPPFSRHPRVLWIGLEESRAL
                     EDLQACLENGLKACGFPRDDRPFHPHLTAARIKSGGIPPPLIGRWAEAEGKPFGRMEV
                     REIVVYRSLLHPGGAEHQPVFSETLRP"
     gene            22730..23326
                     /gene="plsY"
                     /locus_tag="PKV64_03595"
     CDS             22730..23326
                     /gene="plsY"
                     /locus_tag="PKV64_03595"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPN16199.1"
                     /translation="MKWMLLPLSYLLGAFPTGYLFFKWASRQDIRKFGSGATGATNVL
                     RLKGWRLAVPVVVIDVIKGALPAFAAWRITQDPGFAAWCASAAVVGHCFPVFIGFKGG
                     KGVATAAGAMLFLAPVPALLCVGVFVLVVAATRFISLGSMSAAALFPVFGWLFQRPVP
                     LILGSLPFIALILFQHRANIVRLVKGTERKIGEKASLT"
     gene            23323..24348
                     /locus_tag="PKV64_03600"
     CDS             23323..24348
                     /locus_tag="PKV64_03600"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPN16200.1"
                     /translation="MNVACVVGGGSWGTAFSYHLAEAGFRVRLWIREPEICEDAVRTR
                     ENRVFLPGRVFPESASFHNDLKDAAAGADTVFIAVPSIHFRKVYTRLAEFLRPDAGVI
                     SLTKGIEKRSLLRMSEIIASVSLPAGRIRIGVLSGPSFAQEAAGGHPTGLVMASTDAE
                     LARCIQTCASNEYWRIYTSRDLVGVEIGGALKNIIAIAAGLSDGMNFGFNSRAALMTR
                     GLVEISRLGTKLGAKPETFSGLAGMGDLVLTCTGPLSRNRHVGLELGQGRRLADILAG
                     MKGLAEGIPTTVSARQLARRENVDLPITEQVYQVLYRRKDPRRAMTDLMTRTLKDENE
                     SSGSEKP"
     gene            24345..25109
                     /locus_tag="PKV64_03605"
     CDS             24345..25109
                     /locus_tag="PKV64_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN16201.1"
                     /translation="MSRPLRRHAILFLAVALTVPACMSSAKRLALEREKDPQYQFDKA
                     ALCLQYNMVDEAFKYIDQSLALNPRFFPALNLRGLALMMKGRIPESIQALQACLDINP
                     LFSEGWNNLATAYDQSGRKRDAVASWSKAYEIDQNYNAAYNLAKNDYENDHFDAALDW
                     IRKAIAKYPKSVLAFNLEGLIYDAREDYNDAINSFQQAIRLSPTELNVQYNLAVTYYK
                     KKDYGKARELLEKILLQAKDDLRIRAEDLLKRLPRL"
     gene            complement(25119..26285)
                     /gene="metK"
                     /locus_tag="PKV64_03610"
     CDS             complement(25119..26285)
                     /gene="metK"
                     /locus_tag="PKV64_03610"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPN16202.1"
                     /translation="MADHSRYLFTSESVTPGHPDKICDQVSDAIVDDILAQDPNSRVA
                     CETLATTGLVLLAGEITTSAYCDFQKIVRDTVRDIGYTHSHFGFDYETCCVLSSIHEQ
                     SPDIAMGVNESEGHEQGAGDQGLMFGYACRETDVLMPLPIMLAHRLARRLTDVRKGAI
                     LPYLRPDGKTQVTVEYEKGTPKRIEAVVIAAQHDGDVRMADLREDIRAQVIEAVIPAE
                     MIDQKTKFFINGTGRFEQGGPIADSGLTGRKIIVDTYGGFSSHGGGCFSGKDPSKVDR
                     SGSYMARYVAKNLVAAGLADKLEVQLAYCIGIAEPVSLMVDTFGTGKVAEDRIREIIR
                     AHFPLTPKGMIAHLDLKKPRYKKSAVYGHFGRTEPEFTWEKTDKAEAVRRDAGL"
     gene            26385..27215
                     /locus_tag="PKV64_03615"
     CDS             26385..27215
                     /locus_tag="PKV64_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HPN16203.1"
                     /translation="MKHPPYGCPEEFLVHYDCFRTSRPPRLDGRLDDPVWRRAVRSPK
                     FVDLIDGSPAPLETRMAALWDDEAFYVAYWIEEPDVRATLTERDSFLWKENDAELFIA
                     GPDAYYEFQVNARGTIYEVFYVWQDAYRRAGFDRRPEFDLASRNVDVLGGYQDASRYG
                     RHPRGKRWAFMDWDFPGLRWAVNVDGTLNDSRDVDRGWTLEAAFPWEGFALLAGGRPL
                     PPRDGDVWRMDFSRFEPPPGGGSALIPDPGWALNRHGVYDSHVPEAFSCLHFRRRLID
                     "
     gene            27244..28599
                     /locus_tag="PKV64_03620"
     CDS             27244..28599
                     /locus_tag="PKV64_03620"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPN16204.1"
                     /translation="MISRSLGGLRIAAAAAALAVVLAVPSAAQTPFFSGVTHYTLKNG
                     LTVILHDHPDLPIVTVAVGYNAGSIHEKPGKTGVSYLLEKLMMTAGSANVSPLEHISS
                     INRVGGTLNANALEDQTLYYQTIPSNHLALALWLESDRMRTLDLSRDKFEKARLDLLE
                     ELRNRKASEPYLNSLQMFDELLFSDFSFGHSLLGEEADVRRLTLDDVRSFYDSLYTPS
                     NAVLCISGQFNPGRVRELVARYFESLPAAKPPAGGFEAPSYLKAPFSRTIEDPLASAP
                     AVFLGFRLSTPGSSDAPILTLLDFLLLRGRTSRLNRRLLNREAKIAFQLTGGIDQRFD
                     RSAFKIFIVASQPQIQGCFDAVFSELEKIKRSFPSEAEIGRAKMLYRQDFLNRTATSL
                     DRALFLIQSYWSLRAAGRTMDVLEWETERVLRVTSAQIVAIVNRYFTLDNCIILNIRM
                     K"
     gene            28600..30048
                     /locus_tag="PKV64_03625"
     CDS             28600..30048
                     /locus_tag="PKV64_03625"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPN16205.1"
                     /translation="MKRKRGIRIVLSLAAGLGLLTGPAAAQERFRQRLPAPDPRPEFR
                     LPRLESSTLSNGLSMTVVYRENQPITSLHMVFFCGERASPDKTPGLATLAAGIFGQGT
                     QVRPASEIEERIESLGASFAVAADRDVIVLGFHFPEEFLDQALDLVAEMILQPRIGDR
                     EIKIVQSMVSAEISASERDPSFLAHRFLGGLLFQGHPYGKFAFGRGDIKAWTSRDLVA
                     FWNRYIRPNNAQIILTGSVNINTASRKISHALNTWAARAVEAEPLPPLRAPQRARICF
                     LDLPQAGQSVIWAGTVLPPLVPGERFALMAIGQLLGGTINSRLFLNLRESKGYAYETS
                     SGPEFYRGGGAFSVRAVVRPEVVAASVREIQGEIQRLTREPVTLDELEEAKFSMMGGF
                     PLRLDRYDQFSGYVAAMRAAGAGEELWSRFMANAMEVDAERALQAAQKYLSQPFIVVI
                     AGSRAACRESLADFDEVEIYDARGQWIETIRK"
     gene            30061..31737
                     /gene="ftcD"
                     /locus_tag="PKV64_03630"
     CDS             30061..31737
                     /gene="ftcD"
                     /locus_tag="PKV64_03630"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPN16206.1"
                     /translation="MKLVECVPNFSEGRDKTKIAAIAGEIETVPGVKLLDVDPGESTN
                     RTVVTFIGPPDAAAEAAFRAIRKAAEVIDMRRHKGAHSRLGATDVCPFVPLSGVTMEE
                     CVALARALGVRVAAELGIPVYFYEEAAQCHERRNLAAVRQGEYEGLAEKLKDPAWAPD
                     CGEAVFNPSAGATIIGAREFLIAYNIDLNTRDRKLASEIALNIRESGRAKRDKHGDIV
                     RDESGAAVKVPGRFSHVKAVGWYIEDYQRAQISINFTNYKVTPIHVVFDDVRAQAAAM
                     GLRVTGSELVGLIPKDALMQAGRYYLERQGKSSGVPESELLRIAVLSLGLSDVVPFDP
                     AKKIIECQVEESSARLAGLSLQAFADELSMDSPAPGGGSVAAYCAALAAALSAMVANL
                     TVGKKGYEKSWSEMNRLASEAQELKEAFLRYVDLDTAAFNKVMAAHRMPRATEEQARF
                     QEAALEDATREATQIPFEVLRKCDLLVDLAERAVKKGNRNSLSDAGVAALAARAAAEG
                     AWYNVRINLPGLKDEAFRRSTAAEAGEIRRRVRLRAEAVHKRIEKGLEEV"
     gene            31749..32483
                     /locus_tag="PKV64_03635"
     CDS             31749..32483
                     /locus_tag="PKV64_03635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPN16207.1"
                     /translation="MSPKIKLYTSGLKNRLWAVIVLVGAYLVFIALGDLIPQYWDWIG
                     FRAFERSILTLGFVVILVICLSGLALSDKLSSLAIRDGLTGFYNAMYVKARLQEEIDR
                     SIRYKYPLSLLMINPDDFKALNDEFGQGAADQLLKSFSAKLGEIIRASDIVGRIGGDN
                     FLVILPQTNILDAAAVAERIRMETALSDFRLVTRREKVSHFTISVGVYGTPLHKQTAN
                     EVIGLADAAVYRAKKEGKNRVVIYIK"
     gene            complement(32779..>33350)
                     /locus_tag="PKV64_03640"
     CDS             complement(32779..>33350)
                     /locus_tag="PKV64_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPN16208.1"
                     /translation="MKLREAAGIHGDTAIAVVSSLGLAVGVILASVSGRFNASLLGYL
                     FGDILAIEPAEVIFSAALAAGVLAVTAIFYRRLMFMTFDRDAAQAAGVPVRRLDALIT
                     ILTALTIVLGMKIVGILLVSALLVIPAAAGLRSASRFRGAVLGAGLIAGLSIILGLAS
                     AYILDWPASGTIVIAAFLMFGITFVFRKK"
BASE COUNT         6130 a        10614 c        10430 g         6176 t
ORIGIN      
        1 agggcataaa agcgtccgag gccaggacgc atcccgcggg accgtagccg cgacgggaga
       61 tgcggatggc gtcctcggtc gagtcgatgc ggctgcgctg gccggagccg atgccgtgga
      121 tgcggtcttt cgtcccgatg acaacggcat tggagcgggt gaaagccgcg acgttccagg
      181 ccaggagggc ggcctggatt tcctcggcgg acggtttccg gcgggaaacg acgtcgacat
      241 ccgccggcga gacgatccgg gaatcgaagc gcttttggac gagaaggccg ccggcgacgc
      301 gcttcagctc gagtccgttg tccccggccg gtccgtcgat cggtccgccc atccggacga
      361 cccggagcgg cttacgggag gccaggattt ccaaggaatt ttcgacgtaa ccgggggcat
      421 aaacgacctc gatgttccgc gggctttcga ccaggagtcg ggcgacggcc ggagtgacgg
      481 ggacgttgaa gacgtcgacc gagccgaagt tggacagcgg gtccgcgttc caggcttttt
      541 cgaatgtctc gagggcgtcg acgccgaggg cgacgccgct cgggatctcg tgcttgatca
      601 ggacgacggc cgcctggccg gggaacacgg ccgtcagctt gcgggccagc cgctggccga
      661 gatccatgtc gccgacgttg atgtacccca ggcccttgct gccttcctgg aggacttcga
      721 gcgtggccat gttggccccg aaggcgtttt cctcggcgta gaacgccgcc gggtaaccgg
      781 ggttttcccc gtagcgcatg gacatccgct tgtgaaattt ctgttcgccg aaggcaagaa
      841 cgtccgggaa atcctccttg accttggtga agtactgttt ccgggcggct ttgtcggccg
      901 attctttttc agggttcatg cgcctgtcct ttcatgacgg ttgatgagag cgatctccgg
      961 cttccccggc tcgttccggg agaggaagag gcaggccgcg gcgacccgga aatcatcgga
     1021 ccggccctcg ggccgaaggg cttcgtagaa gaccccggcg gtcgccttgg ccgtggtttc
     1081 gacgagagtc agggacagcg gctccccctc gaacacggga agagggttcc ggttcgggcc
     1141 ggcgtaggtg gcgatcatct cggcccgtcc ggggtggagg gaaaattcga aaaacagccg
     1201 ccgggccgac ccgtcgaacc cccgcttgat gaggctgagg gccgctcgtc cgtcgggcca
     1261 gagaagaccg ctgatgcgcg gcgtgtaaat cggggggtcc ggctcataag accaattccg
     1321 cagggccgcg gccagggcgg ccaccgggtg atccgcctcg gttcgggcca cgtcgtcggt
     1381 ctgccggccg ttgctgaccg ccggccgtcc gccgaataga acggccggat agaccagcaa
     1441 gtccggctgt cccgtcttca ggatctcggg atccgtcggc ttcgtcacaa ccgcgtttcc
     1501 ctcccgttcc aggcgccggg cctggcttga gggggagcgt ccggtgaccg catagacgat
     1561 gacatcatga aggccggcgg gatcgcggcc gaggacgacg acgcgtcccg ggtattccat
     1621 gcggcggagg gcttcgagag gatgggttgt catattcgct ctttctgtcg aggagtcggc
     1681 ctgtccggac aaggaaaata tcataccggc ccgggcgaag tcaaaagccg tccggctata
     1741 caaaaaaagg gcattgggct aaaattcggc ctggagaaat gttcatgaca aaaaactgcg
     1801 taatccctac gatcgccttt aaaaacgacg ttttccttca ctcccccgaa gcccggtccc
     1861 tccggattct gagcgagtac ctggagccgg acatgcggtt cgaccggaac aagatcaaca
     1921 gcacggtcct attcctcggt tcggcccggg ttcacgccga cgacgcctcg gctcctcttc
     1981 atccttatta ccgcgacgct gaggagctgg ccttccggct cgcccgctgg gcgatccccc
     2041 tgcggccgac ggggaaaaat tttgtcgtct gcacgggagg cggcccggga atcatggagg
     2101 cggccaaccg cggagcggcc cgggcggggg gaaagtcgat tggcatgaac atctctcttc
     2161 cccacgaaca gtcgcccaat ccctacattc cggaggatct ctgcttcctt ttccattatt
     2221 ttttcatgag aaaattctgg ctcgtcagcc gggcccgggc ggttgtcgcc tttcccggcg
     2281 ggtacgggac gctggacgag ctgttcgaga cgctgacctt gatccaaacg gaaaaaatcg
     2341 cccgcgagga agtcgtggtg ctgctttacg gggaagagtt ctggaaggaa gtcgtccatt
     2401 tcgatgccct tgtgaaatac aaggcgatcg ccccgtccga catgaaaatc ttccatgtcg
     2461 cctcgactcc ggaaaaagcc ttcgcctttc tcaagaagaa gcttctccgg ttctgcagcg
     2521 tctacggccc gccccgtttc cagggataag acggccggaa acggagatac agccgaacgc
     2581 ccccccaagc cgccaggacg cccaggatgt cggcggccgc gtccgcccaa tccgccgttc
     2641 gaccgggcac aaaggattga tggaattcat ccagcccggc ggccgcgatt cccagggcga
     2701 taagagcggc gaaccgaacc gctttttcga ctcggacgcg gttgagtccc tggatcagaa
     2761 gaagcccgaa aacggcgtac aggatgaaat gaaagatctt gtcctgcaag gggaaaagcc
     2821 ggggaattcc gcggatcggc cggtccgaaa gaacatagat caagccgtaa tagagggcgg
     2881 ccgggaggaa ggcggggatt ttccgcatgc ccggctacga tagtcttccc tccgcggtct
     2941 tgtcaagaaa agagcctccc ggggaaattc ttccgccgcg cctccgtcgt ccgctccccc
     3001 aagagggaga gctcccttgg aggaaaccgg atcaccgccg ggggtgaggt cccgaccggg
     3061 aaaatcaacc ccccgttcac ccgcggagac gattcccccc gccttgagcc ttggatatga
     3121 tcagggcatg aaacgcgccg tttccgaccg ccggccgggc ccgggagttc cgcggagcgc
     3181 ggccgttctc ttgctctccc tcctcctgtc cgccccggcc tgcgccggcg ggaggatgat
     3241 cgccggcgcc ccgggacaaa cgggcatagc ctcctggtac gggcccgatt ttcacggccg
     3301 gctgacctcc aaccgggagg tctacgatat gtacgacatg accgcggccc accccaccct
     3361 tcccttcgga acacgggtga tggtcaccaa cctggacaac ggccgggcgg ccgaagtccg
     3421 gatcaacgac cgcggaccgt tcgtcaagga gcggatcatc gacctgtctt acgccgccgc
     3481 ccggatgctc ggcatgatcg gccccggcac cgcaccggtc cgcctggaca ttctcaaccg
     3541 gaaggactgg ccgccgaccg tcggaatggt catccaggtc ggctccttca cgaacgagca
     3601 aaacgcccgg cggctgcagg ccgagctggg ccgccggttc ggcgagacgt tcatttcgac
     3661 ggtcgtctgc tcgggccgcg tgttctaccg ggtccggatc aaagtcgacg atcggtctgg
     3721 cgcggatctt ttagccggcc ggctttcggc ttccggctat cccgttctta tctgcgaagc
     3781 ggattgatcc gctttttcgt ctcctgctcc ggaaaatccc cgccgggttt atcgcccggc
     3841 cgcccgtttt ttccgccggc gatttgagat tccggatgaa aacggctatc ctgccggaaa
     3901 cgccatgacc gacatccgcg gaattttcgc tgaagtcgcc ccgacctatg aacgggccaa
     3961 ccgcgtcctg accttggggc tggaccggcg ctggcgccgg gccgccgccc ggaccgccgc
     4021 cgcgcggggc ggccggctct ggcttgacgt ctgctcggga accggcgaca tggccgagga
     4081 gcttgccggg aggatcccgg ccggcggccg ggtcatcgcc ctcgatttca gcgggccgat
     4141 gatggccggc ctgaaatcca agccgcggtt cgcccggcgc ggggcggccg tccgggccga
     4201 ggcgtccttt ctccccttcc ccgccgggac gttcgacctg gtcaccgtgt cgttcgccgt
     4261 ccgcaatctc aaccgcggcc gggagtccct gcggcggacg ttcgccgagc tccgccgggt
     4321 cttaaaaccg tcgggccgcc tcgtcctggt ggagacgagc cggccgcccg ttcttcccgt
     4381 ccggtggcta tactttttcc tggcgcgcgc cctgatcccg aggatcgggg aaaaaatctc
     4441 cggatcgaag gccgggtacg cttacttggg agcgacgatt ccgcgctttt acggagcccc
     4501 ggagctggcc gtgatcctcg accaagcggg cttcgcccgc gttcgtttcc ggcggctgtg
     4561 cctcggcgcg gccgccgtgc acgtcgcctc cggctagaag gaaatcgaga tggacgttcc
     4621 gccgctgtcg ccgaagcggg gattcaccaa gttgctgttg accgagccga aggtgaagct
     4681 cagcccggcc gaaaggtagt aatcgtatcc cgtcgccagc tgccgccggc ggaggaggat
     4741 ttcctccagc gaagcccctc ccttgggcaa ggccaggaga tcgcggatgc gggctccgcc
     4801 gaaatcgaac gtcaggctga gtcccgaaac gatccgccag gaaacatcgg catcgaaccg
     4861 caggcggttc ttcccaaggt cgtgaaagta atgcgagccg gtgaggacgg cgctcatcga
     4921 gccccagggg cgcttgagtc ccaaggcgat cgacagggtt tgcttccaga gcgtttcctt
     4981 ccgcttgtcg aagatcgtct cctcccggta gcggacgaat tccgggccgg cgtaataaag
     5041 gaggcagagc tgttttttgg tcgcctcgct gtacgggaac aggtcgaatt caacggccgg
     5101 ccggacggcc aggcggaaat cgtaattatt gaaggtcgac gatccggccg tcacggacaa
     5161 tcccaaggac cagtggtcgt tcagactccg gacgaccagc ccggaggcgt tcttggaggt
     5221 ggaaacgttc tcgtagaggt atccctcgta atcgtagctg tttttatcgc ggctgaaatc
     5281 gatcgaggcc cggatcttcc actcgggcgt aacccggttg gcggccaggg cgccgaacca
     5341 ggattgaaag cgggaggact tttggccgtt gagaaaccca ttggcgctca agctgaagac
     5401 ccaaaagttc cagggatcgt ccaccgccgt cggcttgacc gcgtcctcga agagaacgtc
     5461 gagacggccg gcggccggcg tatgggaagc aaagcgaagc aatcccagct gtaaaagccg
     5521 ggcggtttcc ttccgggtcg tttcagccgt ttcgccgggc cgggggcggt agacgagctt
     5581 gtccgtccgc ccggcgaaat cctcccggcc ggaaaattcc aaaacgatga tttctccttg
     5641 ggcgtcgccg gatcggacga tgtccaccac gacccgggcg gatcccgggc cggccgcgac
     5701 cgcggcgaaa gggatttccg aggcatacgc ggcggcctcc gggccggata gaaagacggt
     5761 cagcttctcc ggacgtcccg cgtccggctt caccgggatt tcctgcgaaa aaacagtctc
     5821 cgggaaaacg tgcggaaaaa gcacggcgac cgcggccgcg aggaaaaacg ttttatgaat
     5881 caggcgcatg gtccggttcc tcaattaacg cggatcgtta ctccgccgct gtccccgccg
     5941 ccgagctggc cgaagcgggg attgacgacg ttggtataag ccgagccgaa ggtgaagctg
     6001 aggccgaaga tcgcgaaata gctgtaggag gtggccagct cgcgccgctc aagaaggatt
     6061 tcctcgaggg tggccgttcc ccgggccaga gccagctggt cgtggacgcg ggatccgctg
     6121 gcgaaaagat aggcgctcaa gcctttgacc aagttgagct ggagaaaagc gttcaaggtc
     6181 agattgttct tcttgaaatc gtgaaaataa tgggccccgc tgagggagct ggaaatgaag
     6241 ccccactttt ccttgaggtc gagggtgacg gataacgatt ctccccaaag gcggtcccgg
     6301 gtcttgtcgt agatcgtcgg ttcccggtag cggacattct cgaaccggag gcggtaaaga
     6361 aaacgaagct ggcggcgcga ggattcggcg taggaaaaca ggttgtattc gatcgccggg
     6421 gccggaacga ggcggaaggc gatgtttcca tagctggagg aatcggcgtc cagggcgagc
     6481 ccggccgacc agtgcgggcc caggcttttc accagcagcc cgctggcgcc gtaggactcc
     6541 tgggagctct cgattttcgt tccttggtaa acgaaagagt cgcggcggaa gctggaggaa
     6601 aagccgagac gcagcttcca ctcctcggtc acgcggctgg cggtggcatt gaggccgatg
     6661 gagcctttgg acgcggattc ctccccctcg aggtatccgt cgacggaaat gttgaaaagc
     6721 cagtggttcc agcggtccgg cccggcggcc gggccgttct ccggcgaata acggacctgg
     6781 agacgcgagg cgatgggcgt ccggacgaca taagcggcca ggccttgctt aagcgtccgg
     6841 accagcccct tccggacgtc gtcctcggta tcgtcttttt tggagtaata gcggatcgaa
     6901 tcgtcgatcc cggaaaaacc gtcccggccc atgaacgtca aggtatattc cttgccttcg
     6961 gaagccgttg tttgggttgt gatcaagaca tggacgtcgg cttccttccg gtcccggaca
     7021 taattgacga aagggatttc gtccttgatg tactcgaggt cgcagcttcc gcaatcgatg
     7081 aagatccggg gggcggtttt tttcaacgct tccaagtcat ccggaggcgc ctgggcggcc
     7141 cctggaagtg cggcgaacag gcatcccatc aagacgaaaa cggccggctt cgggattttt
     7201 ttcatgcgtc aatccctttc cttttccgtg tcgcttttcc agatgcgaga tttatgccgg
     7261 aatccttatc caagcttttt caacgggatc aattcttttt acgtgagaat cccttgaaaa
     7321 gttacaaaaa agtgtaaaat agattatctt cgccgcgggg gcgtccgttt ccgcgcctca
     7381 atccaaggcc tctaaaagct ggaggagaac cgcgtgggag ccgtcgggca catcgaattt
     7441 gatccggcgg ccggtaaaaa cgcgcttggg aatgttgtcg atgagcagct ggtacttgcc
     7501 cttgcgaagg aattcgatcc ggaggcggcg gccggccatc gattggattt cgaatgagac
     7561 ttcgttctcc gcgtagagga aatggcggac caccgctccc ccgtcgatcg taatgatcgg
     7621 acggccttct tcctggagga tgatttcttt tcgggaaacg ctcacgtcgt aatggcggcc
     7681 ttggatcgcg acccgggaga tcaagccttc ctcctcgggg ctgatcaggc cgaagcggaa
     7741 tccctcccat ggagtgaagt ccaggtattc ctcgacggcc aggagagcca ggagagggcc
     7801 gaagccgtga tggcgccgcc cggcggcttc cccgctcaac ggatggaagt tttccgggga
     7861 taattgataa ttcttccagg cgttgaaaaa catgacgaag cctttgcggg cgaattccga
     7921 ggcttcggcg tcgaagccat aagccttgag gccttgatag atcaaataat tgatcgccgg
     7981 cccgatcgtc ccccgccaat gctgccggga agggttatcc ggatcgttga aagccgggtc
     8041 atcgcgggag atcgtcgggg cgacgaaatc gccccagaat tcctttggat tgagaaggtg
     8101 cttgcggatg atttgggccc gcgactcgtc cgggatccgg gccagcaagg gaaagaaatt
     8161 cgaggcggcc ttgcgccgcg aaaaccgtcc gtcccagtgc cggtcgaaat aaaatccttc
     8221 ccggctgttc cataaatggt cgttgatcag ctttcggacg gattcgtact ccttgagata
     8281 ggcctcgtgc ccggcccggt cgtccaggat gtcggcgatt tgggacagac accaggcgtc
     8341 caaggcgtac aaactgttga ggtcgacggc attgagcatg aacgttcccg aagccggact
     8401 caccggcaca tcatcccagt tcgggaggtc gtcctggccg gattcccgct tggcccggat
     8461 ttcggccgga gcggcctccg cggaaacgag cttccggtcc gatccccatt ccagcaatcc
     8521 gtccccgttg ccgtcgcggc gcggcttccc ccccggtccc ggctcgagcc aaaaggcgtg
     8581 ccattttttg aggtgcggat aggcgaattc caggagctcc cggtcgcccg ttttgccgaa
     8641 cagcttgaga aggcaaaagg atccgacggg cggctgggac cggtcggggg ttccgccgaa
     8701 tcggctccgc cactggggca tgtttccgtc ggggtattgg gtctcgaggg ccgcccggac
     8761 ggcatcggcg gcgtaacggc tgctttcgag cgagagttcc agggcgttga acagggaatc
     8821 cccggcggaa atcctccagg gatctgaatc ctccggggac cggccggagg cgtcttgccg
     8881 gccggccgag gcgtaaaggc ggtggccgcc ggcttggtaa agcatcatcc agtggaggtt
     8941 gttcgtgacg gcctcggccg ctccgccgta caagccctcc acggccaccc gcttcttctc
     9001 atagcggccc cgctcctcct cgattaaaac atcgatggtt ttggcgttct tataccggta
     9061 caggcgccgc cggacgtttt ccactccgtc cccgacgccg gcggcgaaat aaacggcccg
     9121 tttcccgccg gcgggaaaac tcagaacctc tcccctccct tccgcctcgc cggccggagc
     9181 cccgggcgga tcggtccaaa cgacccacgt cgagggcttc ccctcgtgag actcggccac
     9241 gagctccgcg tccgttgaga tccggcatcg cccctggaaa tgccagggaa aatacgggac
     9301 gaggcggacc gggacgtttt tcgggaacat cagacgcccg acgaccgcgc cctcgtcttg
     9361 ccgggaccat tccaggacaa gcagatcggc ccgccgggaa ggcttgatca aaatcggagt
     9421 gtcttccttc cggtccggca ggaacggaag tgcgagatcg aagccgatcc gggcatatag
     9481 cccgtcgggg gattgagccc cgatttccga ggccagccag aaaaaatcat ctccgtcgat
     9541 ccaccggccg tccttctcga cttggaagcg gaaagcgaac gcggcgtcct tgtggggagc
     9601 cagaacaaga ccgcaccatc cggccgcgtc gagggagccg atgtaagcga ctttccaagg
     9661 atgggcgatt tcaggcgcgg ccggcgttcg aaaagccaga acggccgccc cgattccggc
     9721 cagaagaacc gcggcgcgaa tcaacgcgcg cgccggcgga tcgtttttca tcgattcaaa
     9781 tgtagggcaa agccggcgaa aaatcaacgg ccggggccgt ttccggcgag cggcggcggg
     9841 cgctttttct tcatcgacgg gaagaccgct ccgaatcccg gatcaacgac ggttttcgag
     9901 ggggcgcgcc cccggctccc gcggccgtga aaggattcgg cgcgacagcc gggccgcggc
     9961 cggtttggcg tgccgaaaag gccgacggaa aacgatcccg gcggccgagg gataacgccg
    10021 gatccgtgat cttcacccgc cggcccgaac gcatcggccg acggtccctc tccacacggg
    10081 tttcgacgaa ccgcaggaac cggagcttcc gattaaaaca ggaagcggag gccgaatttc
    10141 cagtagacga cgtggagatt aacccagaga tcggcttggg acagggctcc gcccggatga
    10201 cgggtgatgg tccggccggg ggccgtccgg atcagatagt cgatctcgct gaaaaattcc
    10261 caaagaggcg ataagcgcca ggaggcccca agccccagga agcccgcgcc cccgctgcgg
    10321 acattctgcc gaaccgtggt atccggagga gacaggacgc gcgatccgat ccggaaacgg
    10381 gttccgacgt atccggcccc ggcggcgacg tagggaacaa aatagccgtt tccgcggaat
    10441 tcataccgga gaccgaccag cacgggagtg accgcaaggc ggccctggcg cagcttgccg
    10501 aatgaattcc gggattccgt cgaccagtgg tcgatctcca cggtcgcggc gaggcgcaga
    10561 gtcaggggga aggcgaaccc gaatccggag gccaggccga cgtgaaggtt gttctcctcg
    10621 tgcccggctc ccccggcctg cagggcgaaa gtcggccgcg gggattgggc ctccgccggg
    10681 attccaccca gaatccaggc gagtccgaaa gccgccggga gaatccatcg ctgtttcatg
    10741 agacggcatt gtcgttcatg tcgacggccc tgtcaatgcg ccggccggcc ggatgaatcc
    10801 ggccgcttct tatgataaag tacggaaggt ttcggaggcg tcatgaatct tccgatatat
    10861 tttcgttcgc ggcagggcga gatggccgcc tttctcaagg agcttgtctt ggccgaatcc
    10921 cccaccggag acaagaaggc ggtggacgcc tgcagctccc ttgtcctctc ccggtgcgcc
    10981 cggatcggcg tcaagcacac ccgctatccg ctgcgcgacc ggggagattt ccacctgctc
    11041 gaatacccgt caaaagccga tgatcgcctg gaaggacgcc ttctcctttt aacccatatc
    11101 gatacggttt ggccggtcgg ccgcctggcc cggatgcctt tttatctcca aggcggaaaa
    11161 gtcttcggac cgggcgtcct cgacatgaaa gccgggctcg tcatgtctta tttcgccctt
    11221 cgggcgcttc atgagctcaa cctgaagcct cgccgccgga tcgccctctt catcaattcg
    11281 tccgaggaag cccggtgtcc cgaggccgac gaggtgatcc gaacccaggc gaaagcgtcc
    11341 gaccgagttc tctgcctgga accggccctg ccgggaggag cgctgaaaat gcagcggaag
    11401 ggacggtgcg tcgtccggct ggacgtcagc ggcgtggccg cccacggcgg aaatccggcc
    11461 aagggcgtat cggcgatcga tgagctgatg accgccctcc ggaaaatcca gatgctccgt
    11521 tcggccgata tctcggtcaa cgtcggcctg atcggcggag gggaaaaggc caatgtcgtc
    11581 gccgaggcgg ccttcgccgt ttgcgatatc cgcttctgga cgaacgacca gaaagcccgg
    11641 atcctccggt tcgtcaaagc cctcggatca ggctcccgcc gggcgaaaat ccgggtgacc
    11701 gtcgagggga cgacgccgcc gatggaaaaa acgaggatct cggaaaccct gttccgggaa
    11761 gcccaggcgg tcgcctccga aatgggcgtc cgcctgtccg cggggaaatc gagcggtggt
    11821 tcggacggat cgatcgccgc cggagccggc cgggccgttc ttgacggcct cggcccggac
    11881 ggggacggca tccacgcggc ccacgagcat ctccttcttc cttccttaat cgagcggacc
    11941 gctctgctga ccgcttttct gcagaaattg tgaccgaccg gacagtcccc cgggcgagtc
    12001 ccggcggcgg ccgattgaca ggcgtgaaat ctttgtgtta gcgtaccttc taagattatg
    12061 gaaagagccg tttttcacgg ataatcacca ttatttctca aggaggagcc aatgatcaaa
    12121 aagtactatc ttctctcgcc gggcccgact cccgttccat cgagcgtgct ttcggccgcc
    12181 gccgagccga tcatccatca ccgaacggcg gaattttcca agattttcat ggaggtgacc
    12241 gaaggcttga aatacgtctt cggaaccaag gaagacgttt acgtcctgac gtcctccggg
    12301 acgggggcga tggaagcggc cgtcgccaac ctgtgctcgc cgggcgacca agtcctgacg
    12361 atcaacgccg gaaaattcgg cgagcgctgg ggcaacatct gcaaggcgta cggggtgatc
    12421 aataaggaca tcgttctcaa gtggggcacg gttctgccga aggaagccct tgaagccgag
    12481 ctcaaggcca acccggccgt caaggccgtc ttcgccaccc tgtccgagac gtcggccggc
    12541 cagatcttcg acatccaggg attcggggaa gtcgtggcca agacgccggc cgttctcgtc
    12601 gtcgacggca tttccggcct cggcgcgacg ccctgcccga tggacgaatg gaacgtcgac
    12661 cttcttctaa ccggggccca gaaatccttc atgattcccc ccggcctggc ttacctcgcc
    12721 tgcagcccga aggcctggaa gctcgtcgaa acggccaaat gccagaagtt ttacttcgac
    12781 ctgaagaaag cgcgtaaaaa cctcgaaaag caaacgaccc cttggacgcc cggcgtcacc
    12841 ctcatcatcc agcagaaaaa agccctggag atcatccggg agatgggcct cgagaagctg
    12901 tacgtccatc accgcgtcct cggcgacgcc acccgggccg ggatcaaagc cctcggcctc
    12961 gagcttttgg ccgagcggcc gggcaatatc ctgaccgcgg tcaagacgcc ggccgggatc
    13021 gacggggtca agctcgtcaa aaccatgcag aacaagtata tggcgtatat cgccggcgcc
    13081 caggatccgc acaagggtga attcttccgg atcgcccacc tcggatacat gggcgggttc
    13141 gacatcatca cggccctgac cgccctcgag atgaccctga ccgacttggg ctatcccgtc
    13201 gtcgccggcg cgtccatcaa ggcggcggag ccgattctaa gggagaattg ggcatgaaaa
    13261 aaatcctgat cgccgacgct ctcgacaagg aatccatgga gctgctcaag gccgtccccg
    13321 gttttgacgt cgtcgtcaag accggattgg acgaggccgg cctcatccag acgattcccg
    13381 gattcaacgc cgtcgtcgtc cgcagcgcga gcaagatcac ccggccggtg atcgaagcct
    13441 cctccggact gcagatcgtc gtccgggccg gcatcggctt ggacaacgtc gactcggccg
    13501 cggccaagga aaaaaacatc aaggtcgcca acaccccttc ggccaccacc atcaccgtgg
    13561 ccgagtacgc cttctgcctc atgctgggag cggtccgcca ggtcggccgg gccaacgtga
    13621 ccatgaagca gcacaagtgg gagaaaaaga cccttcacgg cacggagctc tatggaaaga
    13681 ccctcggcct gatcggggcc ggccggatcg gcctggcctt ggccgagcgg gccatcgcct
    13741 tcggaatgcg ggtcctggcg tatgacgtca tccccattca gacgaaactc cccgtcgccc
    13801 aagtcccgct ggacgagctg ctcgcccaag ccgatgtctt ctccctccac gttcccaaaa
    13861 cgaagcaacc gatactgggc ccggccgaat tcgccaagat gaaggacggg gtcgtgatcg
    13921 tcaacgccgc ccgaggcggc gtcatcgatg agaccgcgct cttgaccgct ctgcagagcg
    13981 gcaaggtccg ggccgcggct ctcgacgttt tcgccaagga accgccggag gactggacgc
    14041 tgatcgacca tccgaacgtc acggcctgcc cccacatcgg ggcccaggcc gaggaaggcc
    14101 agaaacgggc cggcctcgag gtcatccgcc tcctcaagga aaccttggcc tgatcccggc
    14161 ctgaggcttc agttttatca cgggcggcgt ctctctttcc ggagggcgcc gcccgttttt
    14221 tttctccttc ttcaatccgc ataatcgata agaatagtat ttttacgccg attcggctaa
    14281 aatattcccc gaccgtcaga caagcgagtg agaagcatgg tctacctgga caacaacgcg
    14341 acgacgcccc tcgacccggc cgtgatcgaa aaaatggcct ggttcatggg cgggcatttc
    14401 ggcaatccgt cctccctcta tcccatcgga cgcgaggtca agaacctgat caacgaggcc
    14461 cgggcccacg tggccgcggc cgtcggagcc gccaaggcgt ccatctattt caccggctcg
    14521 gggaccgaat ccgacaacca ggcgatcttg ggcgtcatcg acgccctgcc cgacaagcgg
    14581 gaattcgtca ccacgaccat cgagcatccg gccgtatccc aaaccgcggc gtacttggaa
    14641 ggcaagggat acaaggtgac ctatgtcccg gtcgacgcga cgggaacgat cgacctggac
    14701 gccctccgct cggccgtcac tccccagacc gccctggtct cggtcatgca cgccaacaac
    14761 gagatcggga cgatccagcc gatcgaggat gtcgtccgga tcgcccgcga gcaaggcgcc
    14821 ctcgtccaca cggatgccgt ccagtccttc ggtaagattc ccttccgggc ggacgacctc
    14881 ggcgtcgatc ttctgaccgt ctcggcccat aagatctacg ggccgaaggg agcgggggcg
    14941 ctctatgtcc gaaaaggaac gcccctgcgc ccgttcatcc acggcggaca ccaggagcgc
    15001 ggcctgcggg ccggtacgga gaacacgatc gggatcatcg gcttcgggga ggccgcccgg
    15061 attctcccgc ttcatttcga agccgactcg gcccgcctgc gccggctggc cggccggctg
    15121 aaagacggcc tggaagccgg cgtgccgaaa atccatttca acggccatcc ggtcaatcgg
    15181 atcccgacca ccctgagcta tgcttttctc ggcttggaat ccgaggccat ccttctttcc
    15241 ctggcggccc aggggattta cgtctccacg ggctccgcct gcagcgagga atccgaggac
    15301 gtttcccccg ttctccgggc gatcggcctc cggcccgaat tcgcccggtc gaccgtccgg
    15361 atgtccctcg gccggttcaa caccgacgac gacgtcaccg agatcctccg ggttctcccc
    15421 gaggtcgtag acaagctccg gaagatctct ccttgggacc cggaggaatt ttaaaaccgc
    15481 gaggatctct gatgagcgcc tctccccttt tcaacatcac cggccggccc attctcctgg
    15541 acggggcctt gggaacggag ctgatgaagg ttcttcccgc gcccggcctt tgtcccgagc
    15601 tcgccaacct cgagcatccc gaggccgtcc gctccgttca ccgggcgtac ttcgaagccg
    15661 gggccgatgc cgtcacgtcc aactccctgg gggccaaccc gttgaagctc gccgccgccg
    15721 gattatccgg ccgggcgcgg gagctggccg cggccgctgt ccggatcgcg gtctccgtcc
    15781 gccccgcggg gaaattcgtg gccggcgaca tcggtccgac aggaaaattc ctcaagcccc
    15841 agggaccggg caccgaaatg gaattcgagg acggattcgc cgtccaagcg gaagggctgg
    15901 ccgagggcgg ggcggacttc cttctgatcg agaccatgta cgacctgcgc gaggccctgt
    15961 gcgcggtccg cggagcccgg cggggcgctc ccgggcttcc ggtcctggcg acgatgacct
    16021 tcaaccggac caaacgcgga tttttcaccc tgatgggtga cgggctggag aaatgcgccc
    16081 gggcgttcga ggccgaggat ttgccggctt tcggagcgaa ctgcaccctg ccgagcggcg
    16141 aaatggccga cctcatggcc gcctggaaac ccctgacgga cacgccgctc atcgcccagg
    16201 ccaacgccgg caagccggaa atcgtcgacg ggcgggttgt ctattctcaa accgaggacg
    16261 agtatctggc cgagatcccc cggctcatcg aaaacggggc ccggatcatc gggggatgct
    16321 gcggaacgac gccgtccttc atccgccgca tggccgtcct catcggggcg gtttgagggt
    16381 ctccaggtac gttttccgga catccaagaa aaaggggatg tgatcctcgg tccgcaggtc
    16441 gggatacgtc cattcgaggc gcttgacccc gttcctgacg aataagagct cgaggtgggc
    16501 gtaaatcccg ttccggagag ggggccgatg ggaaaattcc ttcgtcgtgg ccatgaagag
    16561 agccgcggcc gtcaggatgc cgggatcgag gttgaccgct cttcccggcg tcgcgaagtg
    16621 ctttctcagc tcttcttcca aggcattggt ttcgattttg atatccggca gccgttccgg
    16681 agcgatcaag tcccggaacg agataaaacc ccggctcagg cccggtccca tctcctcttg
    16741 ataatacgcc gtaccggcga agggatagcg aggcccgcgg gcctcgaccg gcccgaatcg
    16801 ttcgcccagg cgacgttcgg cctcggccgc gcgctcctcc cggccggtga tgatcccgca
    16861 gacaagcttg accggggcga agggacggat ctcagccatc tcccttacga taggcgaaat
    16921 cgactggagt ttcaagccgc cctcccctcg ctcgggcttc agtttgcccg ccgtttttag
    16981 tatcctgtag gccgattccg ccgatcgcat tgacagggaa attcaaccaa ggaggtttcc
    17041 gtccattttc gcggggccgg gccgccgggg tttcgccggc gggcatcgag aaaggcctca
    17101 tccgggcgat attcgcttcg gtcgatatca attcagtcat cggctcctcc tcaagcgggg
    17161 caagggagcc gcaaaggaaa acacgatgtc tcaggcttcg aaatggaatg aaacgctgga
    17221 aacgatcgcc gcccgccgga gcatccggcg ctttgacggc cgggaggtcc cccgggagac
    17281 gctggaaatc attctcaatg cggctaatcg cgctccctcg gcccataacc gccaatcctg
    17341 gcggttcctc gtcgtccggg gggagaagaa ggaagagctg gcccgcctgg ccgcctcccg
    17401 ggcggccggt tttccccggc cggcatccgt cctgctccgg atggccgccc gttcgatctc
    17461 cggcgcgccc gtcgtcgtcg cggtggccaa cacgggagag ctgatcgccc acggctggag
    17521 cctttttcaa gggaaagacg agtcgacccg ggactttttc cggatcatgg agatccagag
    17581 ctcggccgcg gccgtcgaaa atcttctgct ggccgcatcc tccctcggcc tggccgcggt
    17641 ctggctcggg atcctgtacc tgatcaagga cgacgtccgc cgccttctcg gcgagccgcg
    17701 cggcgaattc gtggctgtga tcccggtggg gcggcctgcg gtcgagacgc cgagcccggc
    17761 caagcgtccc ctggccgcgg tcgtccgctt cttggagtga gccgcgtttg attttgaggg
    17821 gtcgatctcc tataatgaaa cgaattttcc aaggaatttt ccgccatgaa atgggtttac
    17881 atcgaagacc tcgcctccgc ccgcgaccag gacgttgaaa tccgcggctg ggtgtacaac
    17941 acccggtcga gcggccggat ccggtttctc ctcgtccgcg atggaacggg gatcgtccag
    18001 gccacgatct tcagccggga ggagaacgat ccgttgtttg aaaagttcga cgccctaacg
    18061 caggaatcgt cggtgatcgt ccgcgggcgc gtccgcgagg acaagcgggc tcccggcggc
    18121 gtcgagatcg gggtttccgg aatcgaaatt attcaaatcg ccaaggatta tccgatcacc
    18181 cccaaggagc actccacgcc gttcttgatg gagcaccgcc atctctggct ccgctcgcgc
    18241 aagcagcacg ccgtgctcca ggtccgagcc gaagtcatcc gggcgatccg cggcttcttc
    18301 gacgaccggg gcttccgcct gatggatacg ccgatcctga cgcccagcgc ctgcgagggg
    18361 acgacgaccc tgttcgagac gaaatatttc gaccaatcgg cctacctcag tcagagcggc
    18421 cagctctata acgaagccac ggccgccgct ttcggcaagg tctactgctt cgggccggcc
    18481 ttccgggcgg aaaaatccaa gacccgccgc cacctgatcg agttctggat ggtcgagccc
    18541 gaagtggcct tcgccaccct ggaggacacg atcgccctcg gccaggatct ggtcctgttc
    18601 atcctcgagc gggtcctgac gcggcggcgg gccgacctgg ccgagttgga gcgggatgcc
    18661 gccccgctcg aggcgatcgc cgccccgttc ccccgcctga cgtacgatga ggtgtttccc
    18721 cgtctccagg agaagggatc ggaaatcaag tacggcgacg acttcggcgc gcccgaagag
    18781 accatcctct cggatctcta tgccgccccg gtctgcatca ctcattttcc tgcccggatc
    18841 aaggccttct acatgcagcc cgatgaacag cgtcccgacc tggccttggg cgtggatttc
    18901 ttcgccccgg agggatacgg cgagattatc gggggcggcc agcgcatcca tgacccggac
    18961 cttctcgctg agcgcatccg cgagcacggc ctgcctcccg aaccctacca gtggtacatc
    19021 gacttgagaa aatacgggac gttcccccat tccggtttcg gcctcggcgt ggagcgggtc
    19081 gtggcctggc tttgcgggat taagcatatc cgcgagacca ttcccttccc ccggcttctc
    19141 tacaggatct acccctgagc aaatcccccc gggcggcgat cgtcaccttc ggctgcgcga
    19201 aaaacctggt cgacagcgag gtcatggccg ggcatcttcg aagcgccggc tatgttcttg
    19261 ttccggacac ggcccgggcc gacgttatca tcctaaacac ctgcggcttc atccgcccgg
    19321 cccgtgagga agccgagaaa ggaatccggc gggctttgaa gattaaggcg tcccggccgg
    19381 gcgtccgtct cgtcgtcacg ggttgcgccg tggaaaacga ccggccgggg cttctcgagc
    19441 gccaccccgc cgtcgatgct tggctgggcg tgcgggatta cgaccgcatc gtcgcggccg
    19501 cggaaaaccg gccgttccgg tcccgcgccc ggacgtttct ctatgatcac cggtcccccc
    19561 gcctgctttc gactcccccg ggatgggcgt acctcaaggt ttcggagggg tgttcccatc
    19621 agtgcgcgtt ctgctccatt ccctccatca agggaccgta ccggtcccgg accgtttcct
    19681 cgataatcgc cgaagcccgg ttcttggaag cgcggggagc gcgggaaatc gtcctcgtct
    19741 cccaggacac cacggctttc ggccgggacc gcggccgcgc cgacggattg gccctccttc
    19801 tggcggagct tctgaaagga acggctgtcc cctggatccg ctttctctac ggctatcccg
    19861 aggagatcac cgacgccctt ctcgaagtga tggccgattc ccgcatctgt ccctatctgg
    19921 acattccctt tcagcatgcc tcgcccgccg ttctccgccg gatgaagcga gcctcgtccg
    19981 ccgcgcgtat cctgcgtctc ctcgaaaaga tcaggaaacg cctgcccgaa gccgccattc
    20041 ggacctcgtt gatcgtcggc tttcccggag aagggccgga cgagttcaag cggctccaag
    20101 agttcgtccg ggaggcgcgg atcgaccatc tcggcgtctt cacgtactgc cgggaggaag
    20161 gaacggccgc ttgggctctc ggcgatcccg ttcccgaagc cgagaaggag cggcgccggg
    20221 gcatcctcat ggaagcccag gccgcctatt cagccgaaaa tctgaggaag ttccggggcc
    20281 ggacgctcga tgtcttgatc gagggaccgc ggccggggcg cccggggggg tggatcggcc
    20341 ggaccaagtt ccaggccccg gaagaagacg gagtcgtctt tgtccgggcc gagggcgctc
    20401 gaacatggaa aagcccgctg ggccgtgtta aaatcatttc atccggaaca tacgatcttt
    20461 tcggaaatat cgcgtcatga aatcagtcgt ccgcctggcc tccacgtttt tcggagcggg
    20521 gtattttccc gtggccccgg ggacgttcgc cagcctggaa gccgccttga tctacggatt
    20581 cgccgcccgg cgtcttgctc cccttcccta tctgttgctg ctggcggccg tttttcttgt
    20641 cgggaccgcg gccgccgatt cgttcgcccg gaccttgaac gagaaggatc cgcgtccggt
    20701 ggtgatcgac gagatcgtcg gccaaggcgc ggcgcttttc ctttgcccgg ccggctggcc
    20761 gtggatcgcc gggggatttt ttcttttccg ggtgttcgac gttttgaagc cgtggccgat
    20821 ccgccgcctc gagcgcctgc ccggcggctg gggcatcatg gccgacgacg tcctggccgg
    20881 cgtcttcacc gccctggttc tccaagggat ccgggggatc ctgtgaagcg tgtcgtgatc
    20941 gtggccgtcg ggtcggagat gctgacgccg tttttccagg acaccgattc gcttttcctg
    21001 acccgtcggc tgaatgattt cgggctcgac gtcgaggaga agatcatcgt cggggacgaa
    21061 agcgacagcc tcaagaaagc gatccgggaa ggacgagacc gggccgatct gctcttcgtg
    21121 accggcggac ttgggccgac cgacgacgac cggacccgcg aggccgtggc cgcggtcctg
    21181 aacaaaccgc tggatgtcga cggccgcgtc ctggcggcga tcgaggagcg ttttttccgg
    21241 cgggggaaga cgatgcctcc ggccaaccgg aaacaggccg aggtcatccg cggggccgac
    21301 gtcctggcca atccggccgg caccgccccc ggcctctgga tcgaggagga ggaaaaaatc
    21361 atcgtcctcc ttcccggccc tccgcgcgag cttgaatcgg tttttgaagc ccatgtcgcc
    21421 ccccggatct ccggccggag ccgggcccac ttggcccgga aagtgcttaa ggcggccggt
    21481 ttgaccgaat ccgaggtcga gacgatgatc caggggcttt atccccgcga tccgggccgg
    21541 cgcttgacga tcctcgcctt tcccggacag atcgagctgc atgtctccgc gttttcggat
    21601 gtctcggccg aagaggccga gcggacggcc gagtccctcg cctccgccct ccgggagcgc
    21661 ctgggcccgg ccgttttttc agatgacggc tccgatctcg aagccgtcgt cggccgtctt
    21721 ctcgcggaac ggaaggaaac catcgccgtg gccgaatcgt gcacgggcgg gcttgtctgc
    21781 cggcggttga cccggatcgc gggcagctcg gcgtatttct tggaagggtt ggtcacgtat
    21841 tccaaccagg ccaagatcgg ccggctcggc gtccccgccc ggatgatcga agaacacggg
    21901 gcggtgagcg gggaaaccgc ccgggccatg gccgaaggcg tccggagggt ttccggggcg
    21961 gattacggct tggccgtcac cggcatcgcc ggcccgtccg gagcggccgc gggcaagccg
    22021 gtcggcctgg tgttctgcgc ggcctccggc cccggcggaa cacgcgtgga acgattcctg
    22081 ttccttggag accggagcca tgtccaagcc caggccgccc aagggattct cgacatcctc
    22141 cgccgccggc tcgaggagaa gcccgctcga tgagaacgtt catcgccgtc gatcctcccg
    22201 aggaagtcaa ggcccggatc catgaatttg tccggccgtt ccggcaatcc ttttcccgcg
    22261 ggatcgcctg gtcgagctcc cgcgatctcc acgtcacctt gaaattcctc ggggagatcg
    22321 aggaatcccg gattccgcta gtcaaggaag ccgtccgcag cgccgccgcg gcggccgccg
    22381 ccttccccgt ggccgtcgcc gggaccggct cttttccgcc tttttcccgt catccgcgcg
    22441 tcctctggat cggcctggag gaaagccgcg ccctggagga tctccaagct tgcttggaaa
    22501 acggcttgaa agcctgcgga ttcccccggg acgaccggcc gttccatccc cacctgaccg
    22561 ccgcccggat caagtcgggc gggattccgc cgccgctgat cggccggtgg gccgaggcgg
    22621 agggaaagcc gttcggccgc atggaggtcc gggagatcgt cgtttatcgc agcctgctcc
    22681 atcccggagg ggctgagcat caaccggtgt tttcggagac gctccggcca tgaagtggat
    22741 gctgcttccg ttgtcgtatc tgctgggagc ctttccgacc ggatacttgt tcttcaaatg
    22801 ggcatcgcgc caggacatcc ggaaattcgg tagcggggcg accggagcga ccaacgttct
    22861 tcgcttgaaa ggctggcggc tggccgtccc cgttgtcgtg atcgacgtga tcaagggcgc
    22921 gcttccggcc ttcgccgctt ggcggatcac ccaagatccg ggattcgccg cctggtgcgc
    22981 ctcggccgcc gtcgtcggcc attgttttcc cgttttcatc ggattcaagg gcggaaaagg
    23041 cgtcgccacg gccgccggag cgatgttgtt tctggctccg gtcccggctc tcctctgcgt
    23101 gggcgtgttc gtcctcgtcg tcgccgccac gaggttcatc tccctgggct cgatgtcggc
    23161 ggccgcgctc tttcccgttt tcggatggct tttccaacgg ccggtcccct tgattctggg
    23221 cagccttccc ttcatcgcct tgatcctgtt ccagcatcgc gccaatatcg tccgcctggt
    23281 caaggggacc gaacggaaaa tcggagaaaa ggcttctctc acatgaacgt cgcctgtgtc
    23341 gtcggaggag gcagctgggg aaccgcgttc tcttatcatc tggccgaggc cggcttccgg
    23401 gtccgcttat ggatccgaga gcccgaaatc tgcgaggatg cggtccggac gagagaaaac
    23461 cgggtgtttc tgccgggccg cgtcttcccg gaaagcgcct cgtttcacaa cgatttgaaa
    23521 gacgcggccg ccggcgccga cacggttttc atcgccgtcc cctccatcca ttttcggaag
    23581 gtctacacgc ggctggccga gttcctccgc cccgacgcgg gcgtgatcag cctgactaag
    23641 ggaatcgaga agcgaagcct tctccggatg agcgagatca tcgcctccgt ctcccttccg
    23701 gccggccgga tccgaatcgg cgttttgtcc ggcccgagct tcgctcagga agcggccggg
    23761 ggacatccga cggggctggt gatggcctcg accgacgccg agctggcccg ctgcatccaa
    23821 acatgcgcct cgaacgaata ctggaggatc tacacgagcc gggatcttgt gggcgtggag
    23881 atcggcgggg ccctgaaaaa catcatcgcc atcgccgccg ggctgtcgga cgggatgaat
    23941 ttcggattca actcccgggc cgccttgatg acccgcggct tggtcgaaat ctcgcgcctc
    24001 gggacgaagc tcggagcgaa gccggaaacg ttctcggggc tcgccggcat gggcgatctc
    24061 gtcctgacct gcaccgggcc tctcagccgc aaccggcacg tcggcttgga gctcggccaa
    24121 ggccgccgcc tggccgatat cctggctgga atgaaaggcc tggctgaggg catcccgacg
    24181 acggtctcgg cccgccaact ggcccgtcgg gaaaacgtcg acctgccgat caccgagcaa
    24241 gtgtaccagg tcctctaccg ccggaaggat ccccggcggg cgatgaccga ccttatgaca
    24301 cggactctca aggacgaaaa cgagagctcc ggatcggaga agccatgagt cgtcccctgc
    24361 gccgtcacgc gattcttttc ctggccgtcg ccttgaccgt cccggcctgc atgtcctcgg
    24421 ccaagaggct ggccttggaa agggaaaagg acccccagta tcaattcgat aaggcggctc
    24481 tctgcctcca gtacaacatg gtggacgagg cgttcaagta catcgatcaa tccctggccc
    24541 tcaatccccg ctttttcccc gccttgaacc tgcggggctt ggccctgatg atgaagggaa
    24601 ggatcccgga atcgatccag gccctccagg cctgcctcga catcaacccg ttgttttccg
    24661 agggatggaa caacctggcg accgcctatg atcaaagcgg acgaaaacga gacgccgtcg
    24721 cgtcctggtc caaggcttac gagatcgatc agaactacaa cgcggcctac aacctggcca
    24781 agaacgatta cgaaaacgac cacttcgacg ccgcccttga ttggatccgc aaggcgatcg
    24841 ccaaataccc caagtccgtc ctggctttca acctcgaggg gctgatctat gacgcccggg
    24901 aggactataa cgacgcgatc aacagcttcc agcaggcgat ccgtctctcc cccaccgagc
    24961 tgaacgtcca gtacaacctg gccgtgacgt attacaagaa aaaggattac ggcaaagccc
    25021 gcgagctctt ggaaaagatc ctgctccagg ccaaggatga tcttcggatc cgggcggaag
    25081 acctcctcaa gcggcttccc cgcctgtaat ccggcggatc acaggccggc atcgcggcgg
    25141 acagcctcgg ccttgtcggt tttctcccaa gtgaactcgg gctccgtccg gccgaaatga
    25201 ccgtaaaccg cggatttttt gtaccggggc tttttcagat cgagatgggc gatcatcccc
    25261 ttgggggtga gggggaaatg cgcccggatg atctcccgga tgcggtcctc ggcaaccttg
    25321 cccgtgccga acgtgtcgac catcagggac acgggttcgg cgatgccgat gcagtaagcg
    25381 agctgcactt cgagcttgtc ggccaaaccc gcggccacga ggttcttggc cacgtagcgg
    25441 gccatgtagg acccggagcg gtcgaccttg gagggatcct ttccggagaa acatccgccg
    25501 ccgtggctgc tgaatccgcc ataggtgtcg acgatgattt tccgtccggt caaaccggag
    25561 tcggcgatgg gtccgccctg ctcgaaccgg cccgtcccgt tgatgaagaa tttcgtcttc
    25621 tgatcgatca tttcagcggg gatgaccgcc tcgatgacct gggcccggat atcctcgcgc
    25681 aagtcggcca tcctgacatc gccgtcgtgc tgggcggcga tgaccacggc ctcgatcctt
    25741 ttcggagtcc ctttttcata ctcgacggtg acctgggttt tgccgtcggg ccggaggtag
    25801 ggaagaatcg cgccttttcg gacgtcggtc aggcggcggg ccaggcgatg agccagcatg
    25861 atcggcaggg gcatgagaac gtcggtctcg cggcaggcat acccgaacat caagccctgg
    25921 tcgccggcgc cctgctcgtg accttcgctc tcgttgacgc ccatggcgat gtccggggac
    25981 tgttcgtgga tcgagctcag gacgcagcag gtctcgtaat cgaacccgaa atgggagtgc
    26041 gtgtacccga tgtcccggac cgtgtcccgg acgatcttct ggaagtcgca ataagcactg
    26101 gtcgtgatct ctccggccag caagacgagt ccggtcgtgg cgagggtttc gcaggcgact
    26161 cggctgttcg gatcctgggc gaggatgtcg tcgacgatcg cgtcggaaac ctgatcgcag
    26221 atcttatccg ggtggccggg cgtcacggat tccgaggtga acaggtatcg gctatgatcg
    26281 gccatgaaaa tcctcctccg gtttcgacgc tcaagatcgt caagattagc atacgccgac
    26341 tcccggttgc aatcgcgggt tgattggttt aattctatag cgtcatgaaa cacccccctt
    26401 acggatgccc cgaggaattt ctcgtccatt acgattgttt ccgaacgtct cgcccgcccc
    26461 ggcttgacgg gcggctcgac gatccggtct ggcgccgggc ggtccggtcg ccgaagttcg
    26521 tcgatctcat cgacgggagc ccggccccgc tcgaaacgcg gatggccgcc ttgtgggacg
    26581 acgaggcgtt ctacgtcgct tattggatcg aagagccgga tgtccgggcg actttgaccg
    26641 agcgggattc gtttctctgg aaggaaaacg acgccgagct tttcatcgcc gggcccgatg
    26701 cgtattacga gttccaggtc aacgcccgcg ggacgatcta cgaggttttt tacgtctggc
    26761 aggatgccta ccgccgagcg ggattcgacc ggcggcccga gttcgacttg gcctcccgga
    26821 acgtcgacgt tctgggcggt taccaggacg cctcccgcta tggacggcat ccgcgcggca
    26881 agcggtgggc tttcatggat tgggattttc ccgggcttcg ttgggccgtg aacgtcgacg
    26941 gaacgctcaa cgactcgcgg gacgtcgacc gcggctggac gcttgaagcg gcttttccgt
    27001 gggagggctt cgccctcctg gccggaggcc ggccgcttcc tcctcgcgat ggagacgtct
    27061 ggcggatgga tttttcccgc ttcgagcctc ccccgggagg cggatccgcg ctgatccccg
    27121 atcccggctg ggcgctcaac cgccacggcg tctacgattc ccacgtgccg gaggcttttt
    27181 cttgcctcca tttccgccgc cggctgatag attgaaccgg ccgattgtga tattttaggg
    27241 ctcatgatat cgcggagctt gggagggctg aggatcgccg ccgcggcggc ggccctggcc
    27301 gtcgtcctgg cggttccctc ggccgctcag acgccttttt tcagcggcgt cacccattac
    27361 accctgaaaa acggcttgac cgtcattttg catgatcatc ccgatcttcc catcgtcacc
    27421 gtggccgtcg gctacaacgc cggatcgatc catgagaagc ccggcaagac cggcgtgtcc
    27481 taccttcttg aaaagctcat gatgacggcc gggtcggcca atgtctcgcc gctggagcac
    27541 atctcctcca tcaaccgggt cggcggaacg ctcaacgcca acgccctcga ggaccagacg
    27601 ctgtactacc agacgattcc ctcgaaccat ctggccttgg cgctttggct cgaatccgac
    27661 cggatgcgga ccctcgacct ctcccgggac aagttcgaga aagcccgctt ggacctgctc
    27721 gaggagctgc ggaaccggaa agcctccgag ccgtatctca acagcctcca aatgttcgac
    27781 gagctcctct tttccgattt ctccttcggc cattccctcc tcggcgagga ggcggatgtc
    27841 cgccgtctca ccctggacga cgtccggtct ttttacgact ccctgtacac tccctcgaac
    27901 gccgtcctct gcatctccgg ccagttcaat ccgggccgcg tccgggagct ggtcgcccgg
    27961 tatttcgaat cccttccggc ggccaaaccg ccggccggag gcttcgaagc cccctcctat
    28021 cttaaagccc cattctcccg gaccatcgag gatcccctgg cttccgcccc ggcggtcttc
    28081 ctcggattcc gcctctcaac tccgggatcg tccgacgccc ccatcctgac gctccttgat
    28141 tttctccttc tccgcggccg gacctcgcgg ctcaaccgcc gcctcttgaa ccgggaggcc
    28201 aagatcgcct tccagctgac cggcggaatc gaccagcgct tcgaccggtc cgccttcaag
    28261 atcttcatcg tcgccagcca gccccaaatc caggggtgtt tcgacgcggt cttttccgag
    28321 ctggaaaaaa tcaagcggtc tttcccgagc gaggccgaaa tcggccgggc caagatgctc
    28381 tatcgccagg attttctcaa ccgcacggcg acgtcgctgg accgggcgct ctttctcatt
    28441 caatcgtact ggtcccttcg ggcggccgga cggacgatgg acgtcctgga atgggaaacc
    28501 gagcgggtcc tgcgggtcac atccgcgcag atcgtggcga tcgtgaaccg atatttcacc
    28561 ctcgacaact gcatcattct caatatccgg atgaaataaa tgaagaggaa gcgagggatt
    28621 cggatcgttc tttcgctcgc cgccggactc ggtctcttga ccgggccggc cgccgcccag
    28681 gaacggttcc ggcagcggct gccggcgccg gatccccggc ccgaattccg gctcccccgg
    28741 ctcgaatcga gcaccctgtc caacggtctt tcgatgaccg tggtctaccg cgagaatcag
    28801 ccgatcacca gtctccacat ggtcttcttc tgcggagaga gggcctcccc ggataaaact
    28861 cccggcttgg cgaccctggc cgccggtatt ttcggtcaag gaacacaagt ccggccggct
    28921 tcggaaatcg aggaacggat cgaaagcctg ggcgcctcct tcgccgtcgc ggccgaccgg
    28981 gacgtcatcg tcctcggctt tcatttcccg gaggaatttc tcgaccaagc cctggatctc
    29041 gtcgccgaga tgattctcca gcctcgcatc ggcgaccgtg agatcaagat cgtccagtcg
    29101 atggtcagcg ccgagatttc cgcctccgag cgcgatccgt cgttcttggc ccatcgcttc
    29161 ctgggcggac tcctgttcca aggacatccc tacgggaaat tcgccttcgg gcggggggat
    29221 atcaaggctt ggacgtcccg cgatctcgtc gccttttgga atcggtatat ccgtccgaac
    29281 aacgcccaaa tcatcctgac gggcagcgtc aacatcaaca cggcctcgcg caagatctcc
    29341 cacgccctca acacgtgggc cgcccgggcg gtcgaagccg agccgctgcc gccgctccgg
    29401 gccccgcagc gcgcccggat ctgcttcctt gatcttcccc aggccgggca aagcgtgatc
    29461 tgggccggga cggtccttcc accgctcgtt ccgggggaac ggttcgccct tatggccatc
    29521 ggccaactcc tcggcggaac catcaattcg cgcctgttcc tgaatctgcg cgagtccaag
    29581 ggatacgcct atgagacgtc cagcgggccg gagttttacc ggggcggcgg cgcgttttcc
    29641 gtccgggcgg tcgtccggcc cgaggtggtc gccgcctcgg tccgggagat ccaaggcgaa
    29701 atccagcggc tgacccgtga accggtcacc ctggatgagc tcgaggaagc caaattcagc
    29761 atgatgggag gattccccct gcgcctcgac cggtacgatc aattttcggg ctacgtcgcg
    29821 gcgatgcggg cggccggagc cggcgaggag ctttggagcc gattcatggc caacgccatg
    29881 gaggttgacg ccgaaagggc gctccaggca gcccaaaaat atttgagtca gccgttcatc
    29941 gtcgtcatcg ccggatcgcg ggcggcctgc cgggaaagcc tggccgactt cgacgaagtc
    30001 gagatctatg acgcccgggg acagtggata gagacgatcc ggaagtaagg agaacatgag
    30061 atgaagcttg tggaatgcgt cccgaatttc agcgaagggc gggacaagac gaaaatcgcg
    30121 gccatcgccg gcgagatcga aacagtcccc ggcgtcaaac ttctcgatgt ggatccggga
    30181 gagtcgacca accggacggt cgtgacgttc atcgggccgc cggacgcggc cgccgaggcc
    30241 gctttccggg cgatccggaa agccgccgag gtcatcgaca tgcgtaggca caagggcgct
    30301 cacagccgcc ttggagcgac ggacgtctgt cccttcgtcc ccctttccgg ggtgacgatg
    30361 gaggaatgcg tcgccttagc ccgagccctc ggcgtccgcg tcgccgcgga gctgggcatt
    30421 cccgtctact tctatgagga agcggcccaa tgccacgagc ggcgcaacct cgcggccgtc
    30481 cgccagggcg aatatgaagg actggctgag aagctgaagg atccggcctg ggcccccgat
    30541 tgcggcgaag cggtttttaa tccatcggcc ggggcgacga tcatcggggc ccgggaattc
    30601 ctgatcgcct acaatatcga tctcaacacc cgcgaccgga agctggcctc agagatcgcc
    30661 ttgaatatcc gggaaagcgg ccgggccaag cgggacaaac acggcgacat cgtccgggat
    30721 gaaagcggag ccgcggtcaa ggttcccggc cggttttccc acgtcaaggc cgtgggctgg
    30781 tacatcgagg attaccagcg ggcccagata tcgattaact tcacaaatta caaggtcacg
    30841 ccgatccatg tcgtgttcga cgacgtccgg gcccaagccg cggccatggg gctccgggtc
    30901 acgggaagcg agctggtcgg cttgattccg aaggacgcgc ttatgcaagc cggccggtac
    30961 tatctcgagc gccaaggcaa gagctcgggc gtgccggaaa gcgagttgct tcggatcgcc
    31021 gtcctctccc tcggcctgag cgacgtcgtc cccttcgacc cggccaaaaa aatcatcgaa
    31081 tgccaggtcg aagagtcgag cgcgaggctg gccggcctca gccttcaggc tttcgccgac
    31141 gagctctcga tggactcccc cgccccgggc ggagggagcg tcgccgccta ttgcgccgct
    31201 ttggcggcgg cgctttcggc catggtcgcc aacttgaccg tcggcaagaa aggatatgag
    31261 aaatcctgga gcgaaatgaa ccggctggcg tccgaggccc aggagctgaa agaagccttt
    31321 ctgcgatatg tcgacttgga tacggccgcc ttcaacaagg tcatggccgc tcaccggatg
    31381 ccccgggcga ccgaggaaca ggcccgcttc caggaagccg ccctcgaaga cgccacgcgc
    31441 gaggccaccc aaattccctt cgaggtcctg cggaagtgcg atttacttgt ggatctggct
    31501 gaacgggcgg tgaaaaaagg caaccgcaat tccttgagcg acgccggcgt ggccgccctg
    31561 gccgcccggg ccgcggccga gggcgcttgg tacaatgtcc gcatcaatct gcccggattg
    31621 aaggacgagg ctttccggag gtcgacggcc gccgaagccg gggaaatccg ccggcgggtc
    31681 cgtctccggg ccgaggcggt tcataaaaga attgaaaaag ggctggaaga agtgtaaaaa
    31741 gagaatctat gagcccgaaa atcaagcttt atacttcggg acttaaaaat cggctctggg
    31801 ccgtcatcgt cctggtggga gcttatctcg tttttatcgc cctgggcgat ctcatccccc
    31861 aatactggga ttggatcgga ttccgggctt tcgagcgttc gatcctgacc ctgggattcg
    31921 ttgtcatcct ggtgatctgc ctgagcggcc tggctctcag cgacaagctc tcgagcttgg
    31981 ccattcggga cggcctgacc ggcttttaca acgcgatgta cgtgaaagcc cggttgcagg
    32041 aagaaatcga ccggtccatc cgctacaagt atcccctgag cctgttgatg atcaacccgg
    32101 acgatttcaa ggccttgaac gacgaattcg gccagggcgc ggctgatcag cttctcaagt
    32161 cgttctcggc gaagcttggg gaaatcatcc gggcctcgga catcgtcggg cggatcggcg
    32221 gagacaattt cctggtgatt cttccccaga cgaacattct ggacgcggcc gcggtggccg
    32281 agaggatccg catggagacc gccctgtcgg attttcggct tgtgacccgc cgggaaaagg
    32341 tctcccattt cacgatttcc gtcggcgtct atggaacgcc cttgcataag cagacggcga
    32401 acgaggtgat tggattggcc gacgccgccg tctatcgggc gaaaaaagaa gggaaaaacc
    32461 gggttgtcat ttatatcaag tgaaagcggg gctgtcgcgg agcccggcgg cgccgacaat
    32521 cgctgattcc gatcgcgcga ggcgcgcggt tcgtcatggg ctcggccgac gccgccgttg
    32581 gcttcgtata aaatccgtca tggcaagggc gtggcggcgc cgacaatcgc tgattccgat
    32641 cgcgcgaagc gcgcggttcg tcatgggctc ggccgacgcc gccgtctatc gggcgaaaaa
    32701 agaagggaaa aaccgggttg tcatttatat caagtgaaag cggggctgtc gcggagcccg
    32761 gcggcgccga cctctatcct attttttacg gaagacgaac gttatcccga acataaggaa
    32821 ggcggcgatc acgatcgtcc ctgaagccgg ccaatccaag atgtaggcgg atgccagccc
    32881 gagaatgatc gacaggccgg cgataagacc ggcgccgaga accgctcccc ggaagcggga
    32941 ggcgctccgc agcccggccg cggccggaat caccaacaaa gccgagacaa gaaggatgcc
    33001 gacgatcttc atccccagga cgatcgtcaa agcggtcagg atggtgatca gggcgtccag
    33061 ccggcggacg ggcacgccgg cggcttgggc ggcatcgcgg tcgaaagtca tgaacatgag
    33121 tcggcggtaa aaaatcgccg ttacggccag gacgcccgcg gccagggcgg ccgaaaaaat
    33181 gacttcggcg ggttcaatgg ccaggatatc gccgaaaagg tagccgagaa gggaggcgtt
    33241 gaaccggccg gagacggagg ccagaataac gccgacggcc aagccgaggc tgctgaccac
    33301 ggcgatggcc gtgtcgccgt ggatgccggc cgcctctcgg agcttcatga
//
LOCUS       DAOOXH010000017        32592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_1144_length_32592_cov_7.963734, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000017 DAOOXH010000000
VERSION     DAOOXH010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 32592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32592
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1144_length_32592_cov_7.963734"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..572
                     /locus_tag="PKV64_03645"
     CDS             87..572
                     /locus_tag="PKV64_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HPN16209.1"
                     /translation="MKSETIRSRVRVGVIGGARAKAGPLAQARDVGRLIAGRGAILVC
                     GGLSGVMEAAARGAREAGGLTVGILPGTQPAEANPHIDFALATGLGYSRNALVAMNAD
                     VLIAIDGEYGTLSEIAYGRIHGKPVIGLGSWEIEGVVAASSPEEAVRLAFEALAARSR
                     P"
     gene            569..1297
                     /gene="truA"
                     /locus_tag="PKV64_03650"
     CDS             569..1297
                     /gene="truA"
                     /locus_tag="PKV64_03650"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPN16210.1"
                     /translation="MTNYKIILCYDGTDYLGWQRQPKGRTIQGEVEEALARLAGTRVN
                     IIGAGRTDAGVHALGQAASFKADLKLDDAALFKALNAVLPPDIRILSLERVPAGFHAR
                     RDARSKTYQYRIVTAKAISPFLLRYALHWPHPLNVARMKAAAALFAREADFSGFSSNR
                     ELYPVRRVVRSELRKRGEELLFTIEANGFLRYMIRTIVGTMLEAGRGRVEAGDIENVF
                     RTKARTLANPTAPAKGLCLMQVFY"
     gene            1329..2594
                     /locus_tag="PKV64_03655"
                     /pseudo
     CDS             1329..2594
                     /locus_tag="PKV64_03655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3269..4009)
                     /gene="tsaB"
                     /locus_tag="PKV64_03660"
     CDS             complement(3269..4009)
                     /gene="tsaB"
                     /locus_tag="PKV64_03660"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPN16211.1"
                     /translation="MTASQANRRPESRGNEPPGPKRLLAVDTTTGCGSAALIEDGRVR
                     AEIGVDSFGTHSARLPASIDVLLKSLHWTPADLDGYAVTPGPGSFTGIRIGLSLIQAF
                     AFASGKPIAPVSSLRALAWKLRGDGLPAVCPLHDAKKGEVYAALYLCEKAKMKALVPE
                     GAYAPEAFLKRLPRGRKILFIGGGFPILEAAMSGAEKKRAVLSPRTRFIAPEVGLLGA
                     EIIAAGKGIGPDDLEPLYLRRSQAEEGR"
     gene            4129..4779
                     /locus_tag="PKV64_03665"
     CDS             4129..4779
                     /locus_tag="PKV64_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16212.1"
                     /translation="MRKGFFTAVVAILLLAAGSCGVPKPKLVAREVYTHPEINFGDYK
                     DVAVYVDLANLPWENRNPFFISAIENALVERGFHILGFEGFVDFTRRHQIRADEAMSP
                     RALDLIKKETGKSAVVRFTLNVFVLHAKEADFLRIVTPGVPGFRWGGVTPIDLMLRDR
                     HEWLIDISLAGEMHDTEKALKIWSCSMSGNQTKYEGFIKDFLSQAAGICLDTIPSR"
     gene            complement(4840..5373)
                     /locus_tag="PKV64_03670"
     CDS             complement(4840..5373)
                     /locus_tag="PKV64_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HPN16213.1"
                     /translation="MRETVLRGRVWVLAGADGRLIEDIDTDQIFHNAHLHITEISQMG
                     AFALGNLEGWKDFPEKARPGDILIAGRNFGAGSSRQQAVDCFVALGVAAIAAPSYSAI
                     YFRNAVNTGFPVLRSDGLERAAAEGRIRSGDEVELDLAAGRGRDLTRDGAFPLEPMSA
                     VQREIYEAGSLFRLKKD"
     gene            complement(5375..6625)
                     /locus_tag="PKV64_03675"
     CDS             complement(5375..6625)
                     /locus_tag="PKV64_03675"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HPN16214.1"
                     /translation="MAKTVIEKIVQAHSRDEAAAGRIVWMDLDVRSARDFGGPNVVKH
                     FEREYGDEPLDDPARTAFTFDLCAPACTLKYADNQQACRDFARKRGIPVYDVDRGIGS
                     HVLMEEGLARPGGTAVGTDSHMNILGAIGCFGQGMGDVDITFAFRTGRTWFEVPESVR
                     VVLKGMPSPRATAKDLTLHLCRILGTSKIALRSAEFYGPAAAGLDLAGRITLCSMITE
                     MAGIIGFVPGPVGRPGKTAAAFPGPGTAAPEADPGARYAAEIEVDVDGLEPQVALPPS
                     PNTVKPVSEVRDARIDSGFIGSCTNGRIEDMEAAAAVLRGRKVRPGVMLKIVPATRRV
                     YEEMLSTGILKILFDSGAIVLNPGCGGCAEGHAGLTGKGEVEISTGNRNFPGKQGKGP
                     VYLASPETVAASCVKGEIAAPGEL"
     gene            complement(6645..7760)
                     /locus_tag="PKV64_03680"
     CDS             complement(6645..7760)
                     /locus_tag="PKV64_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HPN16215.1"
                     /translation="MAKYRIAILPGDGVGKDVIEATMPILKKLEVDAEYIYGDIGWEF
                     WRREGNALPDRTKKLLKETDACLFGAITSKPKKDADAELDPSLRGKGLSYFSPIVRLR
                     QEFDLFTNLRPCKAYPGNPLNYKDKIDLVIFRENTEGMYAGVEFRPLPDAVRTALATH
                     PKFKPFAAVPADEIAVSTRIMTQNGCRRIVKAAFDYARAKNRKSVTLVEKPNVLRETG
                     GLMLETAREVAKDYPDIVFKDANVDAMCMWLLKNPYDYEVLVAENLFGDIISDLAAQL
                     VGGLGFACSGNIGSSYAVFEPTHGSAPKYAGMYKVNPIATFLAAELMLEWLGETAKAE
                     ALDGAVAEVIKESRVRTYDMGGKSTSLEMGRAVADKL"
     gene            7837..8820
                     /locus_tag="PKV64_03685"
     CDS             7837..8820
                     /locus_tag="PKV64_03685"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPN16216.1"
                     /translation="MPYSRLILGYCQRLLAAGAFSFVGGEDIPRIIFFAQGRRTPWDG
                     LPDAFLDLDVPPEDDVSFFPLFPWASRRRRSLKEGGEKRVTTVRSNMIPLKDDNPVRI
                     TPLATGALITANVLVFFYQLSLGPQVQRLVYQYGSIPAVILGHGSLPAGLDAIPPVFS
                     LFTSMFLHGGWLHLIGNMLYLWIFGDNIEAAMGRVRYLVFYLACGALASASHIFSNPT
                     SMIPSIGASGAISGVLGAYLMIYPRARVLVLVPLGFFSRILSIPAGFVLGFWFVLQLL
                     EGAMSGAKQGGGVAFWAHVGGFVAGMLLVGLFKKRDVRFFNPPQPRPRNAE"
     gene            complement(9190..9678)
                     /locus_tag="PKV64_03690"
     CDS             complement(9190..9678)
                     /locus_tag="PKV64_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HPN16217.1"
                     /translation="MSTGRVWKYGDDINTDVIFPGRYTYALMSPEEMAKHAMEDLDPE
                     FSKSVQPGDVIVAGKNFGCGSSREQAAACLRAAGVKAVVAKSFARIYFRNAINLGLAV
                     LQCDEAAEVLMTGDPVEIDFAAGTIQSGGKTLRFFPLPESVIGIVQAGGLIESTKKKL
                     GL"
     gene            complement(9675..10928)
                     /locus_tag="PKV64_03695"
     CDS             complement(9675..10928)
                     /locus_tag="PKV64_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HPN16218.1"
                     /translation="MAQTFSEKVLSRKAGRPVRAGEVVTVSPDYCLSHDNSAAIIGEF
                     KKIGVPRVKAPDKIIIILDHIVPAADEKYAQNHKTIREFVREQGLPHFYDIQNGVCHQ
                     VLPESGFALPGFLIMGSDSHTTTYGAFGAFSAGIGRTEAASIWATDEIWLRVPETLRI
                     EFSGRFPENVCAKDAILKLIGDQGADRANYEAVEFAGPAAGEFSLSSRMTLANMAAEM
                     GAKNGYFEPDALTVAWLKARTFREFEVVVSDPGAAYEAVLPFDLSTLEPQVACPHTVD
                     NVKPVRDVAGTPVQQVLIGTCTNGRVEDLAIAARIVKGRKVHPGTRTLVLPASRAVYL
                     EALRNGSIETLSEAGCVILNPGCGPCLGAHQGLLAAGEAVLSTSNRNFRGRMGSRDSA
                     IYLASPATAAASALEGKITDPRIRS"
     gene            complement(10942..11481)
                     /locus_tag="PKV64_03700"
     CDS             complement(10942..11481)
                     /locus_tag="PKV64_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPN16219.1"
                     /translation="MSVSREKILELLPDIGQIADPGLRELTLKIWFEAVKKGGWSEPD
                     LARMPFTLLLKTVSVSLVEHTRNVTRTAVGIGDVLGKEYAGKVRVDRDALLAGALLHD
                     VGKLFEYTLENGAFVKSRAGDLLRHPISGAAFAYQFGLPEEIVHIIAAHSKEGDGGRR
                     TIEAIIVNHADFVNFDVFK"
     gene            complement(11478..12263)
                     /locus_tag="PKV64_03705"
     CDS             complement(11478..12263)
                     /locus_tag="PKV64_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HPN16220.1"
                     /translation="MSDNQEKWPTAITEVKPNRIMARGYLIDEMMGKISFAQAVCLIL
                     TGELPAPAVGKLLDAIFVSSIDHGASPPSALAARTVASTGAELNAAVAAGILAINRFH
                     GGAIEEGQRMFLAIAQKAEASGGDEAAAVKAVLDEMKARGKRASGFGHRLHTKDPRTA
                     KLFGLAEELGLAGRHVRLARLTEEALAAQLGKPLPINVDGAIAALLCDLGIPPAIGNA
                     FFMIARVPGLVAHVHEEKTRMKPMRKIDPAGYVYDGPPERKIP"
     gene            complement(12298..13200)
                     /locus_tag="PKV64_03710"
     CDS             complement(12298..13200)
                     /locus_tag="PKV64_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPN16221.1"
                     /translation="MAIFVDEKSKVLIQGITGREGIARTRLMKDYGTNVLAGVTPGRG
                     GQDVYGVPVYDAVEEAVENVGPIDASVIFVPAAAVKNAALEALAAGVKLLVIVPDRVP
                     IYDVLDIAAAAKDAGARFVGPNTLGLLSPGKAVLGMIGGRAERAREWFQPGPVGVSSR
                     SGGMTSAVAYYLSRAGLGLSTIVHVGGDSIVGSSHPEVMELFERDPETKAVAMFGEIG
                     TTQEERVADLIEHKKFTKPLVAFIGGKAAKSGTRFSHAGAIVDGNRGSYESKVRRLRE
                     VGVHVVEAVADIPGRVAGLLAGKK"
     gene            complement(13216..14778)
                     /locus_tag="PKV64_03715"
     CDS             complement(13216..14778)
                     /locus_tag="PKV64_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPN16222.1"
                     /translation="MARLYEYQSKILLREGGLPVPEGAVASSPAEARAIAERLGRPVV
                     LKIQVWVTGRAGLGGIQFADTPAEAETKAAALLGMKVKNYVVERVLVEEKLAIEAEYF
                     AGIVMDDEAKAPLLVFSSVGGTGIEEIARRRPDRVVRRLIDVRTGLRGFEALNVVRKS
                     GISGPALVPLSDLLTKLAAIARNADARSVEINPLVRTVDGRILAADCHLVVDDYAVYR
                     HPELGIEIAREFDRPPTELERIAYKVEEKDHRGTFYFLQMADQSEPDDLLIGFHGAGG
                     GGSMMSMDAVLARGFKIADYCDTSGNPPASKVYRAAKVILSQPGLKGYFSSGSGVASQ
                     EQYNSARGLVKAFAEERLAIPAVLRLGGNCEEEAIRILSAYLQDLPARVEGYGRDDPP
                     EKCAARLAELIAENRGAVHEIRPMEEPRLPDCAYTFATLTGRLSIDHSRCLHCASKGC
                     LEACGRKILEADAEGLPVLAIPEAEAKKGKCTECLACEIFCRFHEQQAIHIELPIPGL
                     KEYRDRLRREMK"
     gene            complement(14851..16242)
                     /locus_tag="PKV64_03720"
     CDS             complement(14851..16242)
                     /locus_tag="PKV64_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPN16223.1"
                     /translation="MNKSIVLIVEDDPLQRRLIRGNLESEGFVVTDAADQASAVAEMR
                     RLPAEIALVDYKLGRETGLDVIRLLLKENPALAPIMITAFASVETAVEAIKAGAYDYI
                     VKPIDFKKLLLIIDRALERHRLRAEVQSLRETLVDKFSARNFVSASPAMEAVSRLMSK
                     ASQSDANVLITGETGTGKDLVARSIHFASRRAKGPFLAVNIPSLPESLIESELFGAEK
                     GAFTGAGERKTGKFEAAGGGTLLLDEIGDLPLHLQVKLLRFLQDREFSRLGSTAVLRA
                     DVRVLAATNRDLEKAVSEGAFRADLFYRLDVIRIALPPLRERREDIPPLVDHFLRKYA
                     LREKKAVHGISEEALDMLVRHPYPGNVRELENAVERAVVFCENDMILSEDLPRSLTVR
                     SEADSPFPGKAVSLEEKVKRLEIREIREALEASGGVKSAAARKLGITERMLSYKVKIY
                     GLKPKSPEAGNPV"
     gene            complement(16239..17486)
                     /locus_tag="PKV64_03725"
     CDS             complement(16239..17486)
                     /locus_tag="PKV64_03725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPN16224.1"
                     /translation="MRRREFWAFFILPAAGIIVLFFLQSALSREFLKRKTEALVREQL
                     TASARILAAEFEERLKEGVLPQTALDRYSGETDIYFLALVDESGNVLGWSSQFEGYLP
                     IAGRPASDPESWAIDSPAGTILNVVRTFPAKEDRIYSLYLGYSLSRLDEMLAYSRRSA
                     LLLFGALGAVGLLLFSGIYRMHRASLARTEEAAGERQEKERFRELSGFTAGIAHEIKN
                     PLNSLALLCEMMEKNAPDVLAGDAALGRIEVQKISRILDRFSAVLRPLDLRLETIEAD
                     AVIGDAVEAASPAAVSKGIPIRREPGSGPRLRADRGLLAQALFNIVRNGLEASADGEI
                     VIRAEKNKAGVLISVEDSGPGIPAADRERIFEPFFSTKADGLGVGLFLARKIVEAHGG
                     RISAAERRSGGSVFRIELPGEKG"
     gene            complement(17488..17955)
                     /locus_tag="PKV64_03730"
     CDS             complement(17488..17955)
                     /locus_tag="PKV64_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16225.1"
                     /translation="MGLRLGRKVNAGSVVFAVAALLASVQASPPQGRPRMSAHYYDVK
                     TEIRIEGVVQSIRFEPRYQGAAPFLILVLDEPKTSRRFIVEIGPAWFFEHDVHQGERM
                     NVLGSLAPAGDAEAVLIAREVQLQGEIVRVRDARGFPNWRGGSRQAGKRKRGR"
     gene            complement(17928..19139)
                     /locus_tag="PKV64_03735"
     CDS             complement(17928..19139)
                     /locus_tag="PKV64_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16226.1"
                     /translation="MKRFFIFGAAAAVFAGSSSAASLSVSLVQNWTDNLFQTRYAAAD
                     AMTAAGIAWEKSFSEFGLFAGLDYSALRENSGMSLASLSGGADYLQALGDKTALYLSV
                     AGNASLFQAEYSDFNYEGVRIAASLKSYLTPSSIFKAAAVSEFRSYRYAPFDFLSQGL
                     SVSLDKYFSSRTTLQAELGWGYKYFFHPQTIAPEADAAGLVPPEGGGGFGYVGYGNGG
                     YRHGGYGGGNRGGGEYAPVAEAAAGKGIQVASFQARAAQGLGERVGLRLGGIIQWNLS
                     GENPFSTVEEFMMIENPTSDAFSWEGLGWNAQLSALLPWNVSARLGYNTTEMTFPGIE
                     SRDLEGNPLGFTREDKRSRWEISAEKDFGRWSLKLMYAQIRNASNDPLFDWGGGFLSG
                     GLQWGFDWGAK"
     gene            19899..23018
                     /locus_tag="PKV64_03740"
     CDS             19899..23018
                     /locus_tag="PKV64_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPN16227.1"
                     /translation="MSALPPGRICPKCQASNFASDVHCFNCGANLEDETTLSLPDKPD
                     LPAATDPLDFPPGARFAGRYLIVEEIGGGATGRVFKARDTKLDQTVALKMIRPERAAS
                     PEALGFFKKETSLARSLSQENIIRVYDLGESEGVTYLTMECVAGQSLDQLLRASGPLT
                     ETAAVSIARQVCRALAAAHKKGIVHRDLKPQNILLDADGRVRVADFGLARSFELPSGK
                     APGRIVGTPAYMPPEQAAGSEADGRSDIYSLGVILYEMLTGRRPFAAGSAAELLEKHL
                     HEKPKPLRTWNPGLSHRLEQTVLQCLEKDPARRPPTAGRLLQSLEERGKPAPAARRAA
                     RLVLPLGAAALLLAALGLFLVVTRKPARPPAPGTFPSVAVMYFENNTGDPSLDYLRKG
                     LCYQVIQSLLQSARLRVITTDRMFAILRDKGLLKNETYSTDDLKHVAERAQARYILQG
                     SFAKIGRVYKINSFLLDAETWETAGSTQAEVEDLSQLNAAVERMTPAVKASLHSPGGP
                     AAASSSGGLDRITTRSPEALKNFIEGETLFFEGKFAESAEAYRNAIGLDGDFALAYRG
                     LAAASLYQSKRAEAKANLEKALALAAAGRASPRDRHLIEGLYDAVFRSSFPDEAVHQY
                     RQVLSISPDDEEGLRHLGSLYRNLEEWSKARDCWERLLALDKFNPFLYINLSTIYLAE
                     GLPEKAVDILPEDPALLPPALRRQQVGLIDLCRGRYEDALAAVNQALEQESGQTTALL
                     LRGNIRQLQGDAAAAREDYLRIIRDADAASQALGLRFLAALCLQEGRFDEFRSRVAQG
                     LDISRRGAMQDEEDSFLLMEAAGFQLRDNLAAAEESARRAVRLAEELNDVIVRAEGLG
                     LLGHVLLARGNAAGAGETAERLRLLTETSGLRHLRKHYFRLKGAIARQAGDYASAADL
                     GRMAVAGLPFPYSLNDDHAVYYESLALTAETAGDTDRAIGIRRDIGALTSGRLLFGDI
                     YGRNYYRLGKLYLRKNLKAEAADAFDTFLRLWARADAGRPEIADAREELAALKIPGPR
                     PASKTVPAP"
     gene            complement(22969..24042)
                     /locus_tag="PKV64_03745"
     CDS             complement(22969..24042)
                     /locus_tag="PKV64_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPN16228.1"
                     /translation="MLAGLSLTLTERCNWRCRYCPRVRGTNTLEAGTLDQACAALFPY
                     LSSSPLIQFCGGEPLLAFHLIRRAIEQLEKRSRDSRTLPRYSLTTNGSLLRPEILSFL
                     DAHRFALMLSFDGPSQDQERRKGSYRRLTALLDKLPAYPGIRPATNSVFGPRTVGTLA
                     DTIAFLVDRRVPEILVSFDLTVPWPTAALNRLSDGLRAAADALLDYARTGKDVPLLNL
                     RRPPAPGIFVCPAGRDRLALDPEGRLWGCYRFVDFHRAHPRPDVLRRFCFGTVADFVR
                     AGGNPDPRIQAAYSGGRMDLAQTRERPCAGCAWLDRCAFCPPAAAMKSGILGLVPVAH
                     CRISRIVITERERFWRRAAVPES"
     gene            complement(24146..24403)
                     /locus_tag="PKV64_03750"
     CDS             complement(24146..24403)
                     /locus_tag="PKV64_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16229.1"
                     /translation="MQRKRVSKKLSSAPPTLFQMTAYLKEYLGGLRPGRGASEAEKKR
                     YQAAVSCLKEMETVFTKVEGMFERTAARTCPLDRPSISFGG"
     gene            complement(24583..28452)
                     /gene="hrpA"
                     /locus_tag="PKV64_03755"
     CDS             complement(24583..28452)
                     /gene="hrpA"
                     /locus_tag="PKV64_03755"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HPN16230.1"
                     /translation="MTDEAGAPPVRLKWRYPPELPISRRRGDLVQAIRKNRVVIISGE
                     TGCGKSTQIPKMCLEAGRGSRGRIAVTQPRRIAAVTIARRIAEEMGEPLGRTVGYKIR
                     FEEQTSPETRIKVVTDGMLLAETQADGRLREYDTIIIDEAHERSLNIDFLLGILKTLL
                     AKRSDLKVVITSATLDLEKFAAAFDGAPVLEVGGRMYPVRVEYRTPAPDEKEDVDYVD
                     AAAAAVDDLLRREPPGDVLVFMPTEQDILETCEILEGKRYPGFTILPLFARLPGARQG
                     RVYSVAGPKIVVATNVAETSLTIPGIKYVVDTGLARISQYLPGAGINSLPIRPVSRAS
                     ADQRKGRCGRVSDGLCIRLFEEKDYLDRPRFTPPEILRSNLAEVILRMMDLNLGHPAK
                     FPFVDKPTARMIEDGFETLVELGAVRKRGADYHLTDRGRRMARIPLDPRLSRMLIEAA
                     AENCLREIAVIASALSIRDPRERPPAEAARADASHAPFRDPDSDFLTLLNIWDRYHGD
                     WEGLRSSARKRRFCREHFLSFPRMREWTYIHDQILEILREQKFPAVRTKAGSMSRPLY
                     GAIHRAAASGLLSRVAVLKEGKMYTAARGREAMLFPGSSLFGKPPAWIVAAEVVQTGR
                     LYVRTAARIEPGWLESLAGDLCRRAHENPRWDKNRGDVRVEERVTLYGLEIAVHRDVP
                     YGPLDPEAASKIFIREALCAGRVKNPPAFLRRNLQLRDRFEALEEKMRRRDILVPNEK
                     AAEFYARRLPGVYDLKGLSRCLEKQGGDAFLVMAEDDLVQFKPAVSEIALYPDDLSLG
                     GRKIRVQYRFLPGTEEDGATIRIKADLLPRIEIGALEWGIPGYFREKIHALVKGLPQR
                     YRKALVPAAERADTIVREMPRTPGPLSATLSAFVRRRFRADVPADVWESVDVPKHLKI
                     RLVVLDAAGKEAFSGRDYGKLLGLRPKAPVADTASSSWKNAASEWERPAVSDWEWDAI
                     PAEIRLDGGPAAYPALVPGPKDAGLRLFKTAGEAAAVHPRGVEALLSKKFARDLKFME
                     RIMVLPEEHQRAALYFGGKAALEKSLLEGLRVRVFRKEIRTRLEYQEFADRLVKTLFA
                     EGQVLLDAAKRALEAYQAVRADLYTIGMTHQGNKAVEGVLETVRAELDALMPRDFAEV
                     YPAGRLARLPAYLEALRLRTERAKNSPEKDRKKAAEIVPFTEALAGLKKRLGAEGPAE
                     LREELETVRWMIEEYKIAVFAPEVGTVGSISAKRLNEILRDIRGKIEAAVPPPAKPGR
                     PAPGTKPRPAPPAKNKGRSDPRSFRGN"
     gene            complement(28624..29214)
                     /locus_tag="PKV64_03760"
     CDS             complement(28624..29214)
                     /locus_tag="PKV64_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPN16231.1"
                     /translation="MKNVSVFCGSSPGAEPAFASAARDLGREFLRRGLGLVYGGGRVG
                     LMYEIARTIHEGGGRVVGVIPRDMVERELAYAEAGALRIVETMHERKALMAGLSDGFI
                     ALPGGLGTMEEFFEVVTWAQLGIHRKPCGFLNVAGYFDPILGFLVRAEALKFLQPEHR
                     AMILSDPDPARLLDLFETYRPPEADMARWALDMSKT"
     gene            complement(29216..29764)
                     /locus_tag="PKV64_03765"
     CDS             complement(29216..29764)
                     /locus_tag="PKV64_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPN16232.1"
                     /translation="MLWKLFTVFSRVALFSWGGGPASLALMQREATSAGWVTSGEFAD
                     AVALGNALPGPIAPQVSAFIGYKMAGFPGAVMAVTGTVLPATLLMLAMVIAFFKFKDS
                     PSVVSMLRAVRPVVIGFLLWTAYDMAVSVFGLKNAAVGPVLAAHWDKILIAAAAFALL
                     ALTKTNPVWLVVGAAALGLAVY"
     gene            30024..31574
                     /locus_tag="PKV64_03770"
     CDS             30024..31574
                     /locus_tag="PKV64_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011791701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HPN16233.1"
                     /translation="MTGLSVLIALLPVILILVLLIGRKTAADTAGLAGWVAAAAAAGL
                     YFRTPLSIILKSSLGGVLASLPITIMVGTSIYQITVMLETGAISRVVALIKTLAPRDK
                     IIQIMIINVGFGTLLAAMGATPVSILPPIMLGLGYSSFVAIALPALGYDALCTYALLG
                     VPVVVFANFVGLPVEQVGGYFARFMPVITSCIALGMLWIAGGLKTLLKGVFPAVLAGV
                     TAGMIAIGMNQARMVTLTGVAAGLGVVLVMFLYLLIARKPIRDRSVLTDADRASEQRM
                     SLGRAVSPWIILTVFAVLINTPSLPFFRLVFDRWAMPVEIIPGAPEKLRLLWQAYFWI
                     LVSTLLALPFLKPSKAQLSACGRKWLKRAPRPMLASAVFFAIAYIINHSGKGPDWSLA
                     EPGHNMVYVLAAASAQAFDRLYPAVASFLGLLGGFLSGSETSAIAMLTKLHLSTAEKI
                     GAAGVVIAAASGIGGGLASVISPAKLQNAAASIDRIGEEGRVLRTTFVISLFITAVCA
                     AMSLLWAF"
     gene            31664..>32592
                     /locus_tag="PKV64_03775"
     CDS             31664..>32592
                     /locus_tag="PKV64_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16234.1"
                     /translation="MFRKPAAVFIVFLLASLLPPLAAGEADEKESGTPRWIFSFAERI
                     RQESSDNVASLDESKTDSSSYMRMRTNLAVSWKPAETLAFGLRLANEIRYYLSPKTDP
                     KLGINFGLNEVVIDQLYARWDRPAGLPLALTVGRQDIQMGEGFVLMDGGPGDGSRTAY
                     FNGARADVTLASGQTLTAFYVNQPRTDRFLPRLNDVGQIMVEQDEESYGLYHTGGFGK
                     TKFEAYLFRLNRERRGLFPGAGFQTAGGRVQAPLAGRLSLTAEGALQFGRREDDRKTG
                     FGGYARLDYATGAAGALPATLTLGGIWLSGDDPA"
BASE COUNT         5687 a        10637 c        10341 g         5927 t
ORIGIN      
        1 tgcctctttt ctccgccggc ccccgcgggg aaaagaggca aattgaatct ccccggccga
       61 tgtgttaatt aaaagagcga aacatcatga aatccgagac catccgctcc cgcgtccgcg
      121 tcggcgtcat cggcggcgcc cgggccaagg ccggccccct ggcccaagcc cgcgacgtcg
      181 gccggctgat cgccggccgg ggggcgatcc tcgtttgcgg cggactgtcc ggggtcatgg
      241 aagcggccgc gcggggggcc cgcgaagccg gcggactgac ggtcgggatc ctgccgggaa
      301 cccagccggc cgaggccaac ccccatatcg atttcgcctt ggcgacgggc ctgggctaca
      361 gccgcaacgc cctggtggcc atgaacgccg acgtcttaat cgccatcgac ggcgaatacg
      421 ggaccctgag cgagatcgcc tacggccgga tccacgggaa acccgtcatc ggcctcggca
      481 gctgggagat cgagggcgtc gtcgcggcct cgtcgcccga ggaggccgtc cgcctggcct
      541 tcgaagccct tgccgcccgg tcccgtccat gaccaattac aaaatcatcc tgtgctacga
      601 cggaacggat tatctcggct ggcagcgcca gcccaaaggc cggacgatcc agggcgaggt
      661 cgaggaggcg ctcgcccgcc tggccggaac aagggtgaac atcatcgggg ccggacggac
      721 ggacgcgggc gtccatgccc tcggccaggc ggcctcgttc aaggccgatc tcaagctcga
      781 cgacgcggcg ctgttcaaag ccctcaacgc cgttctcccc cccgacatca ggatcctctc
      841 gctggagcgg gttccggccg gcttccacgc ccggcgcgac gcccggagca aaacgtacca
      901 gtaccggatc gtcacggcca aggcgatcag cccgtttctc ctccgctacg cccttcattg
      961 gccgcatccg ctcaacgtcg cgaggatgaa ggccgcggcc gccctgtttg cccgggaggc
     1021 tgatttctcc ggtttttcct ccaaccggga gctctatccg gtccgccgcg tcgtccgctc
     1081 ggaactccgc aaacggggcg aggagctgct cttcacgatc gaggcgaacg ggttcctccg
     1141 ctacatgatc cggacgatcg tcgggacgat gcttgaagcc gggcgcggcc gggtggaagc
     1201 cggcgacatc gaaaacgtct tccggacgaa agcgcggacc ctggccaacc ccaccgcccc
     1261 ggccaagggt ctctgcctga tgcaggtttt ttactaaggg ccggccgcga ggaaagcgcc
     1321 gtcatcgcat gagtaaagcc gatctccaag ccgaacaaag cggcggattc gaaaaccgcc
     1381 ccttcggaca gggacggcgg cggccgtctt tccgccgtct tccccgcaac gtctgggccg
     1441 tgggcttcac cagcttcttc atggatgttt cgagcgaaat ggtcctcaac atcgtgcccc
     1501 tcttcctggc caacgtcctg ggcgtgaaga cggccgtcat cgggctgatc gaggggatcg
     1561 ccgaagccac ggccagcctc ctcaagctgt tttccggatg gctgtcggac aagctggggg
     1621 cccgcaaggg gctggcggtt ctcgggtacg gcctgtcggc cgtcgccaag ccctttttct
     1681 atttcgcccg cggctggggc ttggtcgcgg gagtccgctg ggccgaccgg gcgggaaagg
     1741 ggattcgggc ggcgccccgc gacgcgctgg tggcggactc ggtgaccccg gaaatccggg
     1801 gattcgcctt cggcttccac cgggccatgg acaccgccgg ggccttgatc gggatcctca
     1861 tcgccctcct ggtcgtcgga ctcacccaga gaaactccct gaccttagcc cggtccacgt
     1921 tccagtccat cgttcttctg agcttgattc cggcgttcct ggcggtgctg tccctggccg
     1981 tcggagcgaa ggacgtggcc gccagggacc gccggacggt tccggcgatc tccctgcgcc
     2041 gcatggggaa gccgttttcc atctacctgg cgatcatcag ccttttcacc ctggggaatt
     2101 cctccgatgc gttcctggtt ctccgggctc agaatctggg catgtcggtg accggcatcc
     2161 tgatcatcct ggcgatattc aacctggttt acgccctgat ctccgcgccc gcggggattt
     2221 tctccgaccg cgtcggccgg cggcggttga tcatcggcgg ctggctggtc tatgcggccg
     2281 tctatttcgg cttcgccctc gcccggagag cctggctgat ctggcttctc tacgccgcgt
     2341 acggattgta ttacggcctg gcctacggga ccgccacggc catggtcgcc gacttggtcc
     2401 cggttgatct ccgcggcacg gccttcggga catatcacgc cttgacgggc ctgctggcgt
     2461 ttccctcctc cttgatcgcc ggcatccttt ggcagggcgt cggccggtgg aagggattcg
     2521 gggcgccggc gcccttctat ttcggcggcg cgctggccct cctggccgcg ttcctcatgg
     2581 ccgtctggat gcccaagacc ccggcgagcc tcttcacgag attaaacggc ggctttgatc
     2641 gcagccgccc ggagccatgc cggggccggg acggctgcgc cggcggcgag ggttcgcgct
     2701 cgcgggctct catctcaaaa aaccggggcg cattcgcgga gcggggaccg gccgacagcc
     2761 ggggaatcgg ttttctaggg catgaccggc ccgccggcgc cgaacatcaa gctcatgacc
     2821 accgcatcct cgacgggatt cgtgtaatat tcctttcgga ggccgatggg catgaagccg
     2881 agcttgtcgt aaagggcccg ggcgccggta ttgccggccc ggacttcgag agtgacgaac
     2941 tgaattccat cgtttttcag ggcgtcgaga accttgcgga ggatgttttc ggcgatccgg
     3001 cgcctccggt aatccggatg aacggcgatg ttgttgatct gggcctcgtc gcggatgacc
     3061 cagtagatga tatacccggc gatccggtcc gcttcctcgt ccacggcgac cacgggatgg
     3121 gacaggccga gattctggat ttccccctcg aacgtcgcga gcggccaagg attggggaac
     3181 gaccggcgtt cgatcgccag aacctcgggc aagtcgcggc gcgtcatccg gcgcaagcgg
     3241 atgttcctcg cgccggcgaa aaccgccatc agcgcccttc ctcggcctgc gaccggcgca
     3301 ggtacagcgg ctcgagatcg tccgggccga tgccttttcc cgcggcgatg atttcagccc
     3361 cgaggagccc gacttcggga gcgatgaacc gcgtccgggg cgaaagaacg gcccgctttt
     3421 tctccgcccc gctcatggcc gcctccagga tcgggaatcc gccgccgatg aagaggattt
     3481 tccgcccgcg gggaagccgc ttcaggaacg cctccggggc gtaggcgccc tccggaacga
     3541 gggctttcat tttcgctttt tcacaaaggt acagggccgc gtagacctcg cccttcttgg
     3601 cgtcatgaag agggcagacg gccgggaggc cgtcgccgcg gagcttccag gccagggccc
     3661 ggagggagga aaccggggcg atgggcttgc ccgaggcgaa ggcgaaggcc tgaatcaggc
     3721 tgaggccgat gcggatgccc gtgaacgagc ccggtcccgg tgtgaccgca tagccgtcca
     3781 agtcggccgg cgtccaatgg agggatttga gcaggacgtc gatggaagcc ggaagacggg
     3841 ccgaatgcgt cccgaaggaa tcgaccccga tctcggcccg gacccggccg tcctcgatga
     3901 gggcggccga tccgcatccc gtggtcgtgt cgaccgccag gagccgcttc ggcccgggcg
     3961 gttcgtttcc gcgggattcc ggccgccggt ttgcttggct tgccgtcatg agggcatcat
     4021 acaacaccgg aattccgaga gacaagcttc gtccggccgg ggttgctatt tttctttatt
     4081 cttttcataa tgtctttatc aaaggaatca gcgagaaagg gggaagtcat gagaaaaggt
     4141 tttttcaccg ccgtcgtcgc gatcctgttg ctggccgccg gatcctgcgg cgtcccgaaa
     4201 ccgaaactgg tcgctcgaga ggtctatact catccagaga tcaacttcgg ggattataaa
     4261 gatgtcgctg tctacgtcga cctcgcgaac ctgccctggg aaaaccggaa tccttttttc
     4321 atttcagcca tcgagaacgc cctcgtcgag cggggtttcc atatcctcgg attcgaggga
     4381 ttcgtcgatt tcacccgccg gcatcaaatt cgggccgacg aggctatgag ccctcgcgcc
     4441 ttggatctga tcaagaagga gacgggcaaa tcggccgtcg tccggttcac actgaacgtt
     4501 ttcgttctcc acgcgaagga ggccgatttc cttcgaatcg tgacgccggg agtgccggga
     4561 ttcagatggg gcggcgtaac gccgatcgat ctcatgctcc gcgaccgcca cgaatggttg
     4621 atcgatatct ccctggccgg cgagatgcat gacacggaga aagccttgaa gatttggtcc
     4681 tgctcgatgt ccggaaacca aaccaagtat gaaggtttta tcaaggattt cctctcgcaa
     4741 gcggcgggga tttgcctgga tacgattccc tcccgctgac agcggattag acattctttt
     4801 tcccgatgaa aagagccatc gggcccgcgg aaaacgaagt caatcctttt tcaggcggaa
     4861 caagctcccg gcttcgtaga tctcccgctg gacggcgctc atcggctcga gcgggaatgc
     4921 cccgtcccgg gtgaggtcgc gcccccgccc ggcggcgaga tcgagctcga cctcgtctcc
     4981 cgaccgaatc cggccctcgg ccgccgcccg ctccaggccg tcgctccgga ggacggggaa
     5041 tcccgtgttg acggcgttgc ggaaatagat cgccgaatac gaaggggcgg cgatcgccgc
     5101 cacgccgagg gcgacgaagc aatccaccgc ctgctggcgc gaggagccgg ccccgaaatt
     5161 ccgcccggcg atgaggatat ccccgggccg ggctttttcg gggaaatcct tccagccctc
     5221 caggttgccc aaagcgaacg cgcccatctg ggaaatctcg gtgatgtgga gatgagcgtt
     5281 gtggaagatc tggtcggtgt cgatgtcctc gatcagccgg ccgtcggccc cggccagcac
     5341 ccagacgcgg ccgcgaagaa cggtctctct catgtcacag ctccccgggc gcggcgatct
     5401 cgcccttgac gcaggaggcg gccaccgtct cgggcgaagc caggtagacc gggcccttcc
     5461 cctgcttgcc ggggaaattg cggttcccgg tgctgatttc gacctcgcct tttcccgtca
     5521 gcccggcgtg gccctcggcg catcccccgc accccgggtt gagaacgatg gccccgctgt
     5581 cgaagaggat tttcaggatt ccggtcgaaa gcatctcctc gtacacccgg cgggtggccg
     5641 ggacgatttt aagcataacc cccggccgga ctttccggcc gcggaggacg gcggccgccg
     5701 cctccatgtc ctcgatccgc ccgttggtgc acgacccgat aaagccggag tcgatccgcg
     5761 cgtcgcggac ctcggacacg ggcttcaccg tgttgggcga aggcggaagg gcgacctggg
     5821 gctcgaggcc gtcgacgtcc acttcgatct cggcggcgta tcgggcgccc gggtcggcct
     5881 cgggcgcggc cgtcccgggt cccgggaagg cggccgcggt tttcccgggg cggccgacgg
     5941 gtcccgggac aaagccgatg atgccggcca tctcggtgat catgctgcag agggtgatgc
     6001 ggccggccag gtcgagtccg gcggccgccg gcccgtagaa ctcggccgag cggagggcga
     6061 ttttcgaggt tccgagaatc cggcaaagat ggagggtcaa gtccttggcc gtcgcccgcg
     6121 gcgagggcat ccccttgaga acgacccgga cgctttccgg gacttcgaac cacgtccgtc
     6181 ccgtccggaa ggcgaaggtg atgtcgacgt cgcccatccc ctggccgaaa cagccgatcg
     6241 ccccgaggat gttcatgtgg ctgtccgtgc ccacggcggt cccgccgggg cgggccagcc
     6301 cttcctccat caggacatgg gagccgatgc cccggtcgac gtcgtagacc gggatgccgc
     6361 gcttgcgggc gaaatcgcgg caggcctgct ggttgtcggc atacttcagg gtgcaggccg
     6421 gggcgcagag atcgaaggtg aaggcggttc gggccggatc gtcgagcggc tcgtcgccgt
     6481 attcgcgttc aaaatgcttg acgacgttgg gcccgccgaa atcgcgggcc gaccggacgt
     6541 ccaggtccat ccagacgatc cgcccggcgg cggcttcgtc ccgggaatga gcctggacga
     6601 tcttttcgat gacggtcttg gccacggcgc ctccctttct ccccttacag cttgtcggcg
     6661 acggcccggc ccatttccag cgatgtggac ttgccgccca tgtcgtaggt ccggacccgg
     6721 ctttccttga tcacctcggc caccgccccg tccagggctt cggccttggc cgtctccccc
     6781 agccactcca gcatcagctc ggccgccagg aacgtggcga tcgggttgac cttgtacatc
     6841 ccggcgtatt tgggggccga gccgtgagtc ggctcgaaca cggcgtacga gcttccgatg
     6901 ttgccgctgc aggcgaagcc cagtccgccg acaagctggg cggccaggtc ggagatgatg
     6961 tcgccgaaca ggttttcggc cacgagaacc tcgtagtcat agggattctt caggagccac
     7021 atgcacatgg cgtcgacgtt ggcgtccttg aagacgatgt cgggatagtc cttggccacc
     7081 tcgcgggccg tctcgagcat caggccgccg gtctcgcgca ggacgttggg cttttcaacc
     7141 agggtcacgg acttgcggtt tttcgcccgg gcgtagtcga aagccgcctt gacgatccgc
     7201 cggcagccgt tctgcgtcat gattcgggtg gacacggcga tctcgtcggc cgggaccgcg
     7261 gcgaacggct tgaatttcgg gtgagtcgcc agggccgtcc ggacggcgtc cggaaggggc
     7321 cggaactcga ccccggcgta catgccctcg gtgttctccc ggaagatgac caggtcgatc
     7381 ttatccttgt aattaagcgg gtttccgggg taggccttgc acggccggag gttggtgaac
     7441 aagtcgaatt cctggcgaag gcggacgatg gggctgaaat acgacaagcc cttgccccgg
     7501 agcgagggat cgagctcggc gtcggcgtcc tttttcggct tggacgtgat ggccccgaaa
     7561 aggcaggcgt cggtttcctt gagaagcttc ttcgtccggt cggggagggc gtttccttcc
     7621 cggcgccaga attcccagcc gatgtccccg taaatgtatt ccgcgtcgac ttcgagcttt
     7681 ttcaaaatgg gcatcgtcgc ctcgatcacg tccttgccca ccccgtcgcc ggggagaatc
     7741 gcgattcgat acttcgccat gggatactcc tcacatggat tgcgcgccgg agggggcgcg
     7801 gagggcaatt ttaccacaga acgccggccg cggcgaatgc cgtattcccg cctgattttg
     7861 ggatattgtc aaagattgct ggcggccggc gctttctcct tcgtcggcgg cgaggacatc
     7921 ccgcggatca tctttttcgc tcagggccgg aggacgccct gggacggctt gccggacgcg
     7981 tttcttgatc ttgatgtccc cccggaagat gatgtatctt tctttccgct cttcccttgg
     8041 gcatcgcggc gtcgtcgatc cctgaaagaa ggaggggaaa agcgtgttac aaccgtcaga
     8101 tcgaacatga tcccgctcaa agacgataat ccggtccgga tcacgccgct ggcgacagga
     8161 gcgctgatta cggccaatgt gctggtgttt ttctatcaat tatcgctcgg gccgcaagtg
     8221 cagcgcctgg tttaccagta cggcagcatc cccgccgtga ttctggggca cggaagcctg
     8281 cccgcgggct tagacgccat cccccctgtg ttcagcctgt tcacgagcat gttcctgcac
     8341 ggaggctggc tgcatctgat cggcaacatg ctgtatctgt ggattttcgg cgacaacatc
     8401 gaagcggcga tgggccgcgt ccgatacctc gttttttatc tggcgtgcgg ggcgctggct
     8461 tccgccagcc acatcttttc aaacccgacc tcaatgattc cctcgatcgg ggccagcggc
     8521 gcgatttcgg gcgtgctcgg cgcgtatctg atgatctatc caagggcgcg ggtgctggtc
     8581 ctcgtgccgc tcggcttttt cagccgcatc ctttccatac ccgctggttt cgtccttggg
     8641 ttctggtttg ttctgcagtt gctcgagggc gccatgtccg gcgcgaagca gggaggcgga
     8701 gtggcttttt gggcccatgt cggcggcttt gtcgccggga tgctcctggt cggcctgttc
     8761 aagaaacgag acgtgcgctt cttcaatccc ccccagcccc ggccgcgcaa cgccgagtaa
     8821 aatcccccgt ccggaagttt tccgccgagc gatcaagatc tccgtcaggc cccgaagaaa
     8881 gagtctcccg cctttgaggg cggagctccg gccggtcgat tctccggcgt ccatcagcgg
     8941 tcttgacggc ccccggagga ttacggaatc ctcggggacg acgggctcgg gacgatcgcc
     9001 ccaaaaccgg tgaaggccgc gcgggccgat ttgaccgtcg acgggacggc tcgaccgagt
     9061 gttcaagccc ggcgcgggtc cgagggaagc gcaacccgcc ttaattcaat ggagctcccg
     9121 ataaaaatcg aatgcatttt gggctaaggc gcgggtctct ggtcgacaag gctgaaggct
     9181 gaggctcgct cagagcccga gttttttctt cgtggactcg atcagccccc ccgcctggac
     9241 gatcccgatc accgattcgg gcagggggaa gaaccgaagc gtcttgccgc cgctttgaat
     9301 cgtcccggcg gcgaaatcga tctcgacggg atcgcccgtc atgaggacct cggccgcctc
     9361 atcgcattgg aggacggcca agccgagatt gatggcgttg cggaaataga tccgggcgaa
     9421 ggacttggcc acgacggcct tcaccccggc cgcccgcagg caggcggccg cctgttcccg
     9481 ggagcttccg cagccgaaat tcttcccggc cacgatcacg tcgcccggct ggaccgactt
     9541 ggagaattcc ggatccaggt cctccatcgc atgcttggcc atctcctccg gggacatcaa
     9601 cgcataggtg tagcgccccg ggaaaatgac gtcggtgttg atgtcgtcgc cgtatttcca
     9661 gacgcgtccg gtgctcatga gcgtatcctc ggatcggtga tttttccctc cagggcggag
     9721 gccgcggccg tcgccggcga agccaggtag atggccgagt cgcggcttcc cattcggccg
     9781 cggaaattgc ggttggaggt cgaaaggacg gcctcgcccg ccgccaggag cccctggtgg
     9841 gcgccgaggc aggggccgca gccgggattg aggatgacgc agccggcctc gctcagcgtc
     9901 tcgatcgagc cgttgcgaag agcctcgaga tagaccgccc gggaggccgg aaggaccagc
     9961 gtccgggttc cgggatggac cttccgccct ttcacgatcc gggcggcgat cgccaggtcc
    10021 tcgacccggc cgttcgtgca ggttccgatg agaacctgct ggacgggcgt gccggcgaca
    10081 tcgcggacgg gcttgacgtt gtcgacggtg tggggacagg cgacctgggg ctcgagcgtt
    10141 gaaagatcaa acggcaggac ggcctcgtaa gccgccccgg ggtcggagac gacgacctcg
    10201 aattcccgga aggtccgggc cttgagccag gcgacggtca gggcgtccgg ctcgaagtat
    10261 ccgttcttgg cccccatctc cgcggccatg ttggccaggg tcatccgcga ggacaggctg
    10321 aattccccgg ccgccgggcc ggcgaattcg accgcttcgt aattggcccg gtcggccccc
    10381 tggtcgccga tgagcttgag gatcgcgtcc ttggcgcaga cgttctcggg gaaccggccg
    10441 ctgaactcga tccggagggt ttcgggcacc cgcagccaga tctcgtcggt ggcccagatg
    10501 gaggcggctt cggtccggcc gatcccggcg gaaaaagccc cgaaagcccc gtatgtcgtc
    10561 gtgtgggaat cgctccccat gatcaggaag cccgggaggg cgaagcccga ttcaggcagg
    10621 acttggtggc agacgccgtt ctggatgtca tagaaatggg gcaggccctg ctcccggacg
    10681 aattcgcgga tggttttgtg gttctgggcg tatttttcgt cggccgccgg gacgatgtga
    10741 tcgaggatga tgatgatctt gtccggagcc ttgacccgcg gcacgccgat tttcttgaat
    10801 tcgccgatga tggccgccga gttgtcgtgg gacaggcagt agtcggggga gacggtgacc
    10861 acttcccccg cccggacggg ccggccggcc ttccggctca ggactttctc gctgaacgtc
    10921 tgggccatgg cggaatccct ctcacttgaa gacgtcgaaa ttgacgaaat cggcgtggtt
    10981 gacgatgatc gcctcgatcg tccgccgccc gccgtctcct tccttggagt gggcggcgat
    11041 gatgtggacg atttcctccg ggaggccgaa ctggtaggcg aaggccgccc cggaaatcgg
    11101 gtgtcggagg aggtcgccgg cccggctctt gacgaaggct ccgttctcga gggtgtactc
    11161 gaaaagcttg ccgacgtcgt ggagaagggc cccggccagg agagcgtcgc ggtccacccg
    11221 gacctttccg gcatattcct tgccgaggac gtcgccgatg ccgaccgccg tccgggtcac
    11281 gttgcgggtg tgctcgacga ggctcaccga aaccgtcttg agaaggaggg tgaaaggcat
    11341 ccgggccagg tcgggctccg accatccgcc cttcttgacc gcttcaaacc agatcttgag
    11401 ggtgagctcg cggaggccgg gatcggcgat ctgcccgata tcgggcaaaa gctcgaggat
    11461 tttttcgcgg gaaacgctca tgggatcttc ctttccggag ggccgtcgta aacatagccc
    11521 gccgggtcga tcttccgcat gggcttcatc cgggtttttt cttcgtggac gtgggccacg
    11581 aggccgggca cccgggcgat cataaaaaag gcgttcccga tcgccggcgg aatgcccaaa
    11641 tcgcagagaa gagcggcgat cgctccgtcc acgttgatcg gcaggggctt gcccagctgg
    11701 gccgccaaag cttcctcggt caagcgggcg agacggacgt gccggccggc caggccgagc
    11761 tcctcggcca atccgaacag cttcgccgtc cgcggatcct tcgtgtgcag ccggtggccg
    11821 aaaccggagg cccgcttgcc cctggccttc atctcgtcca gaacggcttt gaccgcggcg
    11881 gcttcatcgc ctccggaggc ctcggccttc tgggcgatgg ccaggaacat ccgctgtcct
    11941 tcctcaatcg ctcctccatg gaaacggttg atggccagga ttccggccgc gacggcggcg
    12001 ttgagctccg ccccggtcga ggccacggtc cgggcggcca gagccgaggg cggggaggcc
    12061 ccgtgatcga tggacgagac aaagatcgca tccagcagct tcccgacggc gggcgcgggc
    12121 agctcccccg taagaatcag gcagacggcc tgggcgaacg agatctttcc catcatctcg
    12181 tcgatgagat atccccgggc catgatccgg ttgggtttga cttcggtgat ggccgtcggc
    12241 catttttctt ggttatcaga catgtcgctt ctcctttgcg gcccggcgtt ccggccgcta
    12301 tttctttccg gccaggagcc cggcgacccg gccggggatg tcggccacgg cctcgaccac
    12361 atggacgccg acttcccgca gccgcctgac cttgctttca taggagccgc ggttgccgtc
    12421 gacgatggct ccggcgtggg aaaaccgcgt ccccgacttg gccgccttgc ctccgatgaa
    12481 ggccaccagc ggcttggtga attttttatg ctcgatcaaa tcggcgaccc gctcctcctg
    12541 ggtcgtcccg atttcgccga acatcgccac cgctttcgtt tccgggtccc gctcgaacag
    12601 ctccatgacc tcggggtggg acgagccgac gatcgagtcc cctccgacgt gaacgatcgt
    12661 gctcaggccg agcccggccc ggctgagata gtaggcgacg gccgacgtca tccctccgct
    12721 tcgggagctg acgccgaccg gtccgggctg gaaccattcc ctggcccgct cggcccggcc
    12781 gccgatcatt cccaggaccg cctttcccgg gctcaggagg cccagggtgt tgggaccgac
    12841 gaatctcgcc ccggcgtcct tggccgcggc ggcgatatcc aggacatcgt agatcggaac
    12901 gcggtcgggg acgatcacca gcagcttcac cccggccgcc aaggcttcca gggcggcgtt
    12961 cttcacggcc gccgccggga cgaagatgac gctggcgtcg atcggcccga cgttctcgac
    13021 ggcttcctcg acggcgtcgt aaacggggac gccgtagacg tcctggccgc cgcggcccgg
    13081 cgtgactccg gccaggacgt tggtgccgta atccttcatc agccgggtcc gggcgatgcc
    13141 ctcccggccg gtgatgccct gaatcagaac tttgcttttt tcgtcgacaa agatggccat
    13201 gaccctatcc gcgcctcatt tcatttcccg ccggagccgg tcccggtatt ccttcagccc
    13261 cgggatcggc agctcgatgt ggatcgcctg ctgttcatga aagcggcaga agatctcgca
    13321 agccaggcat tccgtgcact tgcctttctt cgcctcggcc tcgggaatcg ccagaacggg
    13381 caggccctcg gcgtcggctt caaggatctt ccgcccgcac gcttccaggc agcccttgga
    13441 cgcgcaatgg aggcaccggg aatgatcgat ggacagccgc ccggtgaggg tggcgaacgt
    13501 gtaggcgcag tccggaagcc gaggctcctc catcggccgg atctcgtgga cggcgccgcg
    13561 gttttcggcg atcagctccg ccaaccgggc cgcgcatttc tcgggcggat cgtcgcggcc
    13621 gtatccctcg acccgggccg gcagatcctg aagataggcg ctcaggatcc ggatcgcttc
    13681 ctcctcgcaa ttccccccga gacggaggac ggccggtatc gccaggcgct cctcggcgaa
    13741 cgccttcacc agcccgcggg ccgagttgta ttgttcctgg gaggccacgc ccgagccgga
    13801 ggaaaaatag cccttgagcc cgggctggct gagaatgacc ttggccgccc ggtagacctt
    13861 gctggccgga ggatttccgc tggtgtcgca ataatcggcg attttgaagc cccgggcgag
    13921 cacggcgtcc atggacatca tcgatccgcc gccgccggcc ccgtgaaacc cgatcagcaa
    13981 atcgtccggc tcggattgat cggccatctg gagaaagtaa aacgtccccc ggtggtcctt
    14041 ttcctcgact ttgtaggcga tccgctcgag ctcggtcggc ggacggtcga actcgcgggc
    14101 gatttcaatg cccagctcgg gatgacggta gacggcgtaa tcgtccacga cgagatggca
    14161 gtcggccgct aaaatccggc cgtcgaccgt ccggaccagg ggattgatct cgaccgagcg
    14221 ggcgtcggcg ttccgggcga tcgcggccag cttggtcagc aaatcagaca aggggacaag
    14281 ggcagggccg ctgatcccgg attttcggac gacgttcaga gcctcaaacc cgcgcaggcc
    14341 ggtgcggacg tcgatcagcc ggcggacgac ccggtccggg cgtcggcggg cgatctcctc
    14401 gatccccgtc ccgccgacgg agctgaaaac gaggagcggg gccttggctt cgtcgtccat
    14461 gacgatcccg gcgaagtact ccgcctcgat ggccagcttc tcctcgacca aaacgcgctc
    14521 gacgacgtaa ttcttgacct tcatcccgag aagggcggcg gccttggtct cggcttcggc
    14581 cggagtgtcg gcgaactgga tgccgccgag accggcccgg ccggtcaccc agacctggat
    14641 cttcaggacg accgggcggc cgagacgctc ggcgatcgcc cgggcttcgg ccggggagga
    14701 ggcgacggcg ccttcgggga ccggaaggcc gccttcgcgg agaagaattt tgctctggta
    14761 ttcatacaaa cgcgccaagg gaatctctcc acgaaagaag gtagagggca ttataacgga
    14821 aacgccgccc ggtcgccagc ctaaggggag tcagacggga tttccggcct ccggggattt
    14881 gggcttcagc ccgtagattt tgactttata ggaaagcatt ctctccgtaa tgccgagctt
    14941 ccgggcggcc gcgcttttaa ccccccccga agcctccagg gcttcccgaa tttcccggat
    15001 ttcgaggcgt ttgaccttct cctcgagaga aaccgccttc cccgggaacg gggaatcggc
    15061 ttcggaacgg acggtcaagg accgcgggag atcctcgctt agaatcatgt cattttcaca
    15121 gaagacgacg gctctttcga cggcgttttc cagctctcgg acatttcccg gatacggatg
    15181 acggaccagc atgtctaagg cttcctctga aatcccgtgg acggccttct tctcgcggag
    15241 cgcgtatttc ctgagaaaat gatcgaccag cggcggaata tcctcccggc gctcgcgaag
    15301 cggcggaagg gcgatccgga tgacatccaa ccggtagaac aagtcggctc ggaaagcccc
    15361 ctcgctcacg gccttctcca ggtcccgatt cgtcgccgcg agaacgcgga catcagcgcg
    15421 gaggactgcg gtcgagccca ggcgggaaaa ttcgcggtcc tggagaaagc gcaggagctt
    15481 gacttgaaga tggagcggaa ggtcgccgat ctcatccagg agaagcgttc cgcctccggc
    15541 cgcctcgaat tttcccgtct tccgctcgcc ggccccggta aacgctcctt tctccgctcc
    15601 gaaaagctcg ctttcaatca gcgattcggg gagagagggg atgttgaccg ccagaaaggg
    15661 acccttcgcc cgcctcgagg cgaaatgaat cgaccgggcg acgagatcct ttcccgtgcc
    15721 ggtctccccg gtgatgagga cattggcatc gctttgagac gctttcgaca tgagccgcga
    15781 cacggcctcc atggccggcg aggcggaaac gaaattgcgc gcgctgaatt tgtcgaccag
    15841 ggtttcccgc aggctttgca cctcggcccg aaggcgatgc cgctccaagg cccggtcgat
    15901 gatcagaagc agcttcttaa aatcgatcgg cttgacgata tagtcataag ccccggcctt
    15961 gatcgcctcg accgcggttt cgacgctggc gaacgccgtg atcatgatcg gggccagggc
    16021 gggattctcc ttaaggagaa gacggatgac gtcaaggccc gtctcccggc cgagcttgta
    16081 atcgaccaag gcgatctccg ccgggagacg ccgcatttcc gccacggccg acgcttgatc
    16141 cgccgcgtcc gtcacgacga agccttcgct ctccaaattc ccccggatga gcctccgctg
    16201 gagaggatca tcctcgacga tcaggacgat cgatttattc atcctttttc ccccgggagc
    16261 tcgatccgga agacggatcc ccccgatctt cgttcggcgg ccgaaatccg cccgccgtgg
    16321 gcttccacga tcttgcgggc cagaaagagg ccgactccca atccatcggc tttcgttgag
    16381 aaaaacggct caaagatgcg ttcccggtcc gccgccggga tgcccggccc ggaatcctcg
    16441 acggaaatca aaacaccggc cttgttcttt tcggctcgga tgacgatctc cccgtccgcg
    16501 gaagcttcga gcccgttgcg gacgatattg aacagggcct gggcgagcaa gccacgatcc
    16561 gcccggaggc gcggcccgga gcccggctcc cgccggatcg gaatgccctt ggaaaccgcg
    16621 gcgggggaag ccgcctcgac cgcgtctccg atgacggcgt ccgcctcgat cgtttccagc
    16681 cggagatcca acggccgaag cacggccgag aaccggtcga ggatgcggga gattttttgg
    16741 acctcgatcc ggccgagggc cgcgtcgccg gccaggacgt ccggggcgtt cttttccatc
    16801 atctcgcaga gaagagcgag gctgttcagc ggattcttaa tctcatgggc gatcccggcg
    16861 gtgaatccgg acagctcgcg gaatctttct ttttcctggc gttcgcccgc ggcttcctcg
    16921 gtccgggcca aggaagcccg atgcatccga taaatcccgc tgaagagaag aaggcccacg
    16981 gcgcccaggg cgccgaaaag aaggagggcg cttcgccgtg aataggccag catttcatcc
    17041 aaccgcgaca gggaatatcc aagatagaga gaatagatcc ggtcttcctt cgccgggaat
    17101 gtcctcacga cgttgagaat cgtcccggcg ggggaatcga tggcccagct ttccggatcc
    17161 gaggcgggcc ggccggcgat cggaagatat ccctcgaatt gagaggacca gcccagcaca
    17221 ttcccgctct cgtcgacaag ggctaagaaa tagatgtccg tctccccgga gtaacggtcg
    17281 agggcggttt gaggaagaac cccctctttg agccgctcct caaattcagc ggccaggatg
    17341 cgggcggaag cggtcaactg ctccctgacc agggcctcgg tttttcgctt gaggaattcc
    17401 cggctcaggg cggactgaag gaaaaagaga acgatgattc cggccgcggg aaggatgaaa
    17461 aaagcccaaa actcccgtcg gcgcaattca acggccccgc ttccgtttcc ccgcctgccg
    17521 agagcctccc ctccaattcg ggaatccccg ggcatcccgg acccggacga tctccccctg
    17581 gagttggact tccctggcga tcaaaaccgc ctctgcgtct cccgccggag ccaacgatcc
    17641 gaggacgttc atccgctcgc cctgatggac gtcatgttcg aaaaaccagg ccgggccgat
    17701 ttcgacgatg aaacggcggc tcgtcttggg ctcgtccaaa accaggatga gaaagggagc
    17761 cgctccctga taacgaggct cgaatcggat ggattgaacg acgccctcga tccggatttc
    17821 cgtctttacg tcataataat gagcggacat ccgcggccgg ccttgcggcg gggaagcctg
    17881 gacggacgcc aaaagcgcgg cgacggcgaa aacgacggat cccgcgttta ctttgcgccc
    17941 caatcgaagc cccattgcaa tcctcccgaa aggaagcccc cgccccagtc aaaaagcgga
    18001 tcgttggacg cgtttcgaat ttgagcgtac atcaacttca acgaccaacg gccgaaatcc
    18061 ttttcggccg aaatctccca acgggagcgt ttgtcttccc gggtgaagcc gaggggattc
    18121 ccctccaggt cccggctctc gatcccggga aaagtcatct ccgtcgtatt atagcccagg
    18181 cgggcgctga cgttccaggg aagaagagcc gacaactggg cattccagcc caagccctcc
    18241 caggaaaagg catcggaggt cgggttttcg atcatcatga attcctcgac cgtggaaaag
    18301 ggattttccc cggatagatt ccattgaatg atccctccca ggcgcagccc caccctctcc
    18361 cccaatcctt gggcggcccg ggcttgaaac gaggcgactt gaatcccttt ccccgccgcg
    18421 gcttcggcga ccggagcgta ttccccgccc cctcggtttc cgccgccgta tcctccatgc
    18481 cgatatcctc cgtttccgta cccgacgtat ccgaaaccgc cgcccccctc cggtggaacg
    18541 aggccggccg catcggcttc cggggcgatc gtctgcggat ggaaaaagta tttatatccc
    18601 cagccgagct cggcttggag cgtcgtccgc gaggagaagt acttatccag cgaaacggac
    18661 aagccctggc tgagaaaatc gaacggcgca tagcgatacg accggaattc cgaaaccgcc
    18721 gccgccttga aaatggaaga gggcgtcaaa tagcttttca aagaagccgc gatgcggacg
    18781 ccttcgtagt tgaagtcgga atattcggcc tggaatagcg aggcgtttcc cgcgaccgag
    18841 agatacaggg ccgttttatc tccgagcgcc tgaaggtaat ccgcgcctcc cgacaaagac
    18901 gccaggctca ttcccgaatt ctctcggaga gcgctgtaat cgagaccggc gaaaaggccg
    18961 aactccgaga aggatttctc ccaggcaatg ccggccgcgg tcatcgcgtc cgcggcggcg
    19021 taccgggttt ggaagaggtt gtccgtccag ttttgaacga gagacacgga aagcgaagcg
    19081 gcggacgatg accccgcgaa aacggcggcc gcggcgccga agataaaaaa tcgtttcatg
    19141 atcagccccg gtcgttatga ttataaggga aaaagggcct tccggccctt tttccccgtc
    19201 attgtctgga acggccggct cgagattccc gccttatcca cggccccggc ggacggatcg
    19261 gccgtgagga cccgtgccgt cgcaaaagcc cggtcccgtt cctctgccgc ggaacgccgc
    19321 tttattccaa gagtttgcga ttccctcggc ccggacaccc ccccgccgat tcccgttctt
    19381 gtagcccgtc ccatccttgg gaggcgccca atccgggtcc cggccgttgg ggatgccgtc
    19441 attgtcatgg tcccggaaac ggtcattgat cccgtcgcaa ttttgatcca tgaacttgtg
    19501 ttggaaccgc cactggaaat gattccgggc attgatttcc tttccaggcg aattcggggc
    19561 tgtcgcggcc agggctccgg cgccgagaac gccgaggaca aggatcgatc cgatgataaa
    19621 accgtttttg agtctcatgg tttaacctcc ttcatgagtt caagcctcat gtatgcggga
    19681 ggcatgccaa attatatatc attgataatc aataagataa ttataatata aagacagagg
    19741 ggccgacatt ttcgtcgatg aaacgacgga attgtcggaa tccggggatt ccgattcgat
    19801 aactccgctc ccatcggcgc ggtattctcc ttccccgaaa ttgacttcat gattccgtct
    19861 ttctatattg ttaataacaa tcggaaaagg gccagcggat gagcgccttg ccgccgggca
    19921 ggatttgtcc caaatgccag gcgtccaatt tcgcgtccga tgtccattgc ttcaactgcg
    19981 gggcgaatct cgaggacgaa acgacgcttt ccctgcccga taagccggac ctccccgcag
    20041 cgaccgatcc gctcgatttt cccccgggag cgcgtttcgc cggccgctat ctcattgtcg
    20101 aggaaatcgg cggcggagcc acgggccggg tcttcaaagc ccgggacaca aagctggatc
    20161 agaccgtcgc cctcaagatg atccggccgg agcgcgccgc aagtcccgag gccctcggct
    20221 tctttaaaaa ggaaacctcg ctggcccggt cgctctccca ggaaaacatc atccgcgtct
    20281 acgacctggg cgaaagcgaa ggcgtgacct acctcacgat ggaatgcgtc gccgggcaga
    20341 gcctggatca gctcctgcgc gcctccggcc ccctgaccga gacggcggcc gtctccatcg
    20401 cccgccaagt ttgccgggcc ctggccgccg cccacaagaa aggaatcgtc caccgggatc
    20461 tcaagccgca aaacatcctg ctcgacgccg acggccgggt ccgggtggcc gatttcgggc
    20521 tggcccggtc gttcgagctc ccgtcgggga aagccccggg ccggatcgtc ggcaccccgg
    20581 cctacatgcc gcccgagcag gccgcgggga gcgaggctga cggccgttcc gatatttatt
    20641 ccctgggcgt catcctctac gaaatgctga ccggacgaag gcctttcgcc gccggaagcg
    20701 ccgccgagct tctcgaaaaa cacctccatg aaaaaccgaa gccgctccgg acctggaatc
    20761 ccggcctctc ccaccgcctg gaacagacgg tcctccaatg tcttgaaaaa gatccggccc
    20821 ggcgcccgcc gacggccgga cgccttctcc agtccttgga ggaacgggga aaaccggccc
    20881 ccgccgcccg gcgggccgcc cgcttggttc tccccctcgg ggcggccgcc cttctcctgg
    20941 ccgccctcgg cctgtttctc gtcgtcaccc ggaaaccggc ccggccgccg gcccccggaa
    21001 cgttcccctc cgtggcggtc atgtatttcg agaacaacac gggcgatccg agcctcgatt
    21061 acctccgcaa gggattatgc tatcaagtca tccaatccct tctccaatcc gcccggctgc
    21121 gggtcattac gaccgaccgg atgttcgcca tcctccggga caagggcctt ctcaaaaacg
    21181 agacgtattc caccgacgac ctcaagcacg tggccgaacg ggcccaggcc cgctacatcc
    21241 tccagggcag cttcgcgaaa atcggccggg tatacaagat caactctttt cttttggacg
    21301 cggaaacctg ggagacggcc ggttcgaccc aggccgaagt ggaggacttg agccagctca
    21361 acgccgccgt cgagcggatg acccccgcgg tcaaggcgtc cctccattcc cccggcggcc
    21421 cggcggcggc ctcgtccagc ggggggctcg acaggatcac cacccggtcc cccgaggcgc
    21481 tcaagaattt catcgagggc gagacgctgt ttttcgaggg gaaattcgcg gaaagcgccg
    21541 aggcttaccg gaacgcgatc gggctcgacg gagacttcgc cctggcttac cgcggcctgg
    21601 ccgcggcctc cctgtaccaa agcaagcggg ctgaagccaa agccaacctg gaaaaagccc
    21661 tggccctggc cgcggccggc cgggcttcgc cgcgcgaccg gcatctgatc gaagggcttt
    21721 acgacgcggt gttcagatcg tcgtttcccg acgaagccgt ccatcagtac cggcaagtcc
    21781 tttcgatctc ccccgacgac gaggaggggc tccgccacct gggttcgctt taccggaatc
    21841 tcgaggaatg gagcaaggcc cgggactgct gggagcggct cctggccctg gacaaattca
    21901 atccctttct ctatatcaac ctctcgacca tttacttggc cgagggattg cccgaaaaag
    21961 ccgtcgacat tcttcccgaa gatcccgcct tgctgcctcc ggccctgcgc cgccagcagg
    22021 tcggcctcat cgatctctgc cggggccggt acgaagacgc cctggccgcg gtcaaccaag
    22081 ctttggagca ggaaagcgga caaaccacgg cacttcttct ccgaggaaac atccgccagc
    22141 ttcagggtga cgcggccgcg gcccgagagg attatcttcg gatcatccgc gacgccgacg
    22201 ccgcctctca agccctcggg ctgaggtttt tagccgctct ttgtcttcaa gaaggccgct
    22261 tcgacgaatt ccggagccgg gtcgcccagg gactcgacat ctcccgccga ggggcgatgc
    22321 aggacgagga agattccttt ctcctgatgg aagcggccgg ttttcaactt cgggacaatc
    22381 ttgccgcggc cgaggaaagc gcccgccggg ccgtccgcct ggccgaggag ctgaacgacg
    22441 tgatcgtccg ggccgaaggt ctcggccttc tggggcatgt cctgctcgct cggggcaatg
    22501 ccgccggggc cggggaaacg gccgagcgtc tccgcctcct caccgaaaca tccggccttc
    22561 gccatctccg gaagcattat ttcaggctga agggagcgat cgcccggcag gcgggagact
    22621 acgcgtccgc cgccgacctg ggccggatgg ccgtcgccgg attgccgttc ccctattccc
    22681 tgaacgatga ccacgccgta tactacgagt ctttggccct caccgccgag acggcgggag
    22741 atacggacag ggcgatcggg atccgccggg acatcggcgc cctgacctcg ggacgtcttc
    22801 tcttcgggga catctacggc cggaactact accgcctggg aaaactctat ctccggaaaa
    22861 atctcaaggc cgaagccgcc gacgcgttcg atacgtttct ccgcctgtgg gcccgggccg
    22921 acgccggccg gcccgaaatc gccgacgccc gggaagagct ggccgccctc aagattccgg
    22981 gaccgcggcc cgcctccaaa accgttcccg ctccgtgatc acgatccggc tgatccggca
    23041 gtgggcgacg gggaccaggc cgaggatccc gctcttcatg gccgcggccg gcgggcagaa
    23101 ggcgcagcga tcgagccaag cgcagccggc gcacggccgt tcccgcgttt gggcgaggtc
    23161 catccgcccg ccgctgtaag cggcctggat ccgcggatca ggattcccgc cggcccggac
    23221 gaaatccgcg accgtcccga aacagaaacg gcggagcacg tccgggcggg gatgggcccg
    23281 gtggaaatcg acgaaccggt agcagcccca cagccgcccc tcggggtcca gggcgaggcg
    23341 atcccgtccc gccggacaga cgaatatccc gggagccggc ggccggcgga gattcaacaa
    23401 cggcacgtcc ttcccggtcc gggcatagtc caaaagagca tccgcggcgg cgcgaagacc
    23461 gtcggaaagc cggttgagag cggccgtcgg ccaggggacc gtgagatcga acgacacgag
    23521 gatctccggg acgcgccggt cgacgaggaa ggcgatcgta tccgccagcg tgcccaccgt
    23581 ccggggacca aagacgctgt tggtggccgg ccggatgccg gggtaggcgg ggagcttgtc
    23641 taagagggcg gtcagcctcc gataactccc tttccgcctt tcttggtcct gggacggacc
    23701 gtcgaagctc agcatcagag cgaagcgatg ggcgtcgagg aaagagagga tttccggcct
    23761 gagaagggag ccgttcgtcg tcagggaata gcgcgggagc gtccgggaat cccggctcct
    23821 cttttccagc tgctcaatcg cccgccggat caagtgaaaa gccaggagcg gctccccgcc
    23881 gcaaaactgg atcaggggag acgaggagag ataaggaaag agggccgcgc aggcctggtc
    23941 caaggtcccg gcttcgaggg tattcgtccc ccgaacccgg gggcaatagc ggcaccgcca
    24001 gttgcagcgc tccgtcaggg tcagggaaag gccggccagc actccccttc ccggcgggct
    24061 cttccgctcc ggccgaacgc gagccgcggc cggccgggac tcaaagcggc gggccgggtc
    24121 gattccgccg gaaaacggaa gaaggctagc cgccaaagga gatcgacggc cgatcgagcg
    24181 gacaggtgcg ggccgctgtt cgctcgaaca ttccctccac tttcgtgaaa accgtctcca
    24241 tctccttgag gcaagagacg gccgcttgat agcgtttctt ttcggcttca gaagctcccc
    24301 gtccgggccg gaggcccccc aggtattcct tcaggtaagc ggtcatctgg aagagggtgg
    24361 ggggggccga ggatagtttt ttcgacactc ttttcctttg catggcattc ctcctgtttg
    24421 gcttcaatat gcgccgaaga aaaagcgttg tcaacggccg aggagacccc cgaacacgag
    24481 gcgggaaagg acggacccgg cctcgtttcc cgcgccgcgg gggcgcggga aggatatgag
    24541 cggaaggaca tgcggcggtc ttgaaaaaga agcgggcgct tctcaattcc cgcggaagga
    24601 gcggggatcc gagcgcccct tgtttttcgc cggcggcgcg ggacgcggct tggtcccggg
    24661 cgcggggcgt cccggcttcg ccggcggggg aacggcggct tcgattttcc ctcggatgtc
    24721 ccgcaagatc tcgttcaggc gcttggccga aatggagccg acggtcccga cctcgggagc
    24781 gaaaaccgcg atcttgtatt cctcgatcat ccaccggacg gtctccagct cctcccgaag
    24841 ctcggccggg ccctcggccc caagccgttt tttcagtccg gccagcgcct cggtgaaggg
    24901 aacgatttcg gccgcttttt tccgatcctt ttccgggctg ttcttagccc gctcggtccg
    24961 aagccggaga gcctcaaggt aggccggaag acgggccagc cggccggcgg ggtagacttc
    25021 ggcgaagtct cggggcatca gggcgtccaa ctcggcccga accgtttcca aaaccccttc
    25081 caccgctttg tttccctggt gggtcatgcc gatcgtgtaa agatcggccc ggacggcttg
    25141 gtatgcctcg agcgcccgct tggccgcgtc caaaagaacc tgtccctcgg cgaacagcgt
    25201 tttcaccagc cggtcggcga actcctggta ttcaagccgc gttcggatct ccttgcggaa
    25261 cacgcggacg cggagcccct cgagaaggga cttttccaag gccgctttcc cgccgaaata
    25321 cagggccgcc cgctggtgct cctcgggcaa gaccatgatc cgctccatga acttgaggtc
    25381 ccgggcgaat ttttttgaga gaagggcttc gacgccgcgc ggatggacgg ccgccgcctc
    25441 cccggcggtt ttgaaaagcc ggaggcccgc gtctttcggt ccggggacaa gcgccggata
    25501 ggccgccggc ccgccgtcga gccggatttc cgcgggaatc gcgtcccatt cccagtccga
    25561 gacggccggc cgctcccatt cgctcgcggc gttcttccag gaggaggacg cggtgtcggc
    25621 cacgggcgct ttcggccgca agccgaggag cttgccgtaa tcgcgtcccg agaacgcttc
    25681 ctttccggcc gcgtccagga cgaccagccg gatcttgaga tgcttcggca cgtcgacgga
    25741 ttcccaaacg tccgccggaa cgtcggcccg gaaccgtctc cggacaaagg cggagagagt
    25801 cgcggacaac gggcccggcg tccggggcat ctcccggacg atcgtgtcgg cccgttcggc
    25861 cgccgggacg agggctttcc ggtagcgctg gggaagcccc ttgacgaggg cgtggatttt
    25921 ttcccggaaa tatcccggga tcccccattc gagggcgccg atctcgatcc gggggagaag
    25981 atccgccttg atccggatcg tcgctccgtc ctcctccgtt ccggggagga agcggtactg
    26041 aacccggatt ttccgcccgc ccaggcttag atcgtcggga tacagggcga tttcagaaac
    26101 cgccggcttg aactggacga gatcgtcctc ggccatgaca agaaacgcgt caccgccctg
    26161 tttctccagg catcggctga ggcccttgag gtcgtaaacg ccgggcaagc gccgggcgta
    26221 aaattccgcg gctttctcat tcgggacgag gatgtcccgc cggcgcattt tttcctcgag
    26281 cgcctcgaag cggtcccgga gctggaggtt gcggcgaaga aacgccggag gattctttac
    26341 ccggcccgcg cacaaggcct cgcggatgaa gatcttcgag gcggcctcgg gatcgagggg
    26401 gccgtaggga acgtcccgat ggacggcgat ctccaggccg tagagggtca ctcgttcctc
    26461 cacccggaca tcccccctgt ttttatccca ccggggattc tcgtgcgccc tccggcagag
    26521 atctccggcg agcgattcga gccagccggg ttcgatgcgg gcggccgtcc ggacatacag
    26581 ccggccggtt tggacgacct cggcggcgac gatccaggcc ggcggtttcc caaacagcga
    26641 ggaaccgggg aagagcatcg cttcccggcc gcgggcggcc gtgtacattt tcccttcctt
    26701 gaggacggcc acccgggaca acaggccgga ggccgccgcc cggtggatcg ccccgtacag
    26761 gggccgggac atggagccgg ctttggtccg gacggcggga aatttctgct cccggagaat
    26821 ctcgaggatc tggtcgtgga tgtatgtcca ttcccgcatc cgcgggaagg aaaggaaatg
    26881 ctcccggcag aaccgccgct tccgggccga ggaccggagg ccttcccaat ctccatgata
    26941 gcggtcccaa atgttcaaga gcgtcaagaa atcggaatcg ggatcgcgga agggcgcgtg
    27001 ggaagcatcg gcccgggcgg cctcggcggg cggccgctcc cggggatcgc ggatgctgag
    27061 ggcggaggcg atgaccgcaa tctcgcggag acagttctcc gcggccgcct cgatcagcat
    27121 ccgggagagg cgcggatcga gcggaatccg ggccatcctc cggccgcggt cggtcaggtg
    27181 gtaatcggcg cccctcttcc ggaccgcgcc cagctcgacc agggtctcga agccgtcctc
    27241 gatcatccgg gccgtcggct tgtcgacgaa cgggaacttc gccggatggc cgaggttgag
    27301 atccatcatc cggaggatga cctcggccag gttcgaacgg aggatctcgg gcggagtgaa
    27361 ccgcggccgg tccaagtaat ccttttcctc gaaaagccgg atgcacaatc cgtcgctcac
    27421 ccggccgcag cggcccttcc gctggtcggc gctggcccgg gagaccggcc ggatcggaag
    27481 gctgttgatg cccgccccgg ggaggtactg ggagatccgg gccaggcccg tgtcgacgac
    27541 gtacttgatg ccggggatcg tcagggacgt ttcggccacg ttggtggcca cgacgatctt
    27601 cggtccggcg acggaataaa cccgtccctg ccgggccccc ggcaggcggg cgaacaaggg
    27661 cagaatcgtg aatccgggat agcgcttgcc ctcgaggatc tcgcaggttt ccaggatgtc
    27721 ctgctccgtc ggcataaaaa caaggacgtc gccgggcggc tcgcgccgca ggaggtcgtc
    27781 gacggcggcc gcggccgcgt cgacgtaatc gacgtcctcc ttttcgtccg gcgccggcgt
    27841 ccggtattcg acgcggacgg gatacatccg gccgccgacc tcgagcacgg gcgcgccgtc
    27901 gaaggcggcc gcgaattttt cgagatcgag ggtggccgag gtgatcacca ccttaagatc
    27961 ggaacgcttg gccaggaggg tcttgagaat ccccaggagg aagtcgatgt tcaggctccg
    28021 ctcgtgggcc tcgtcgatga tgatcgtgtc gtattcacgg aggcggccgt cggcctgggt
    28081 ttcagccagc agcattccgt cggtcacgac cttgatccgc gtttcgggcg aggtttgttc
    28141 ctcgaagcgg atcttgtagc cgacggtccg gccgagcggt tcccccatct cctcggcgat
    28201 ccgccgggcg atcgtcacgg ctgcgatccg gcggggctgg gtaacggcga tccggccgcg
    28261 cgacccgcgg ccggcttcca ggcacatctt cgggatctgg gtgctcttcc cgcagccggt
    28321 ctcgccggaa atgatgacga cccggttctt gcggattgct tggaccaggt cgccgcggcg
    28381 gcgggaaatc ggaagctcgg ggggatacct ccacttcagc cggaccggcg gtgcgccggc
    28441 ttcatccgtc atggcccgcc tcccggccgt ggatcgttca tgggccttca ttctggcacg
    28501 cggccgcggg ggagtcaatt acaatgagga gacataagac ctattgattt cattgcgcgt
    28561 cccccggcgg cagaggattg gatctcggga aatcaggtta attggtattg tgtcccctct
    28621 ttatcatgtc ttgctcatgt cgagggccca gcgggccata tcggcctcgg gcggccggta
    28681 ggtttcgaaa agatcgagca ggcgggccgg gtccggatcg gagaggatca tcgcccggtg
    28741 ctcgggctgg aggaacttca acgcctccgc ccggaccagg aatcccagga tcggatcaaa
    28801 atagccggcg acgttcagga aaccgcaggg ctttcggtgg atgccgagct gggcccaagt
    28861 cacgacttcg aagaactcct ccatcgtccc gaggccgccg ggcagggcga tgaatccgtc
    28921 ggacaatccg gccatcaggg ccttgcgctc gtgcatcgtc tcgacgatcc ggagggcgcc
    28981 ggcctcggcg taggcgagct cccgctcgac catgtcccgg gggatgacgc cgacgacccg
    29041 gccgccgccc tcatgaatcg tccgggcgat ctcgtacatc agacccaccc ggccgccgcc
    29101 gtaaacaaga cccaagccgc gacggaggaa ttcccgcccc aggtctcggg cggcggaggc
    29161 gaaggccggt tccgctcccg ggctcgaacc gcagaaaacg gacacgtttt tcatctcaat
    29221 acaccgccaa gccgagggcc gccgctccaa ccacgagcca aacgggattg gtctttgtca
    29281 acgcgaggag ggcgaaagcc gccgcggcga tgaggatttt atcccagtga gccgccagga
    29341 ccggtccgac agccgcgttt ttcaacccga acacgctcac ggccatgtcg taagccgtcc
    29401 agagaaggaa gccgatgacg accggacgga ccgcgcggag catggaaacg acggagggag
    29461 aatccttgaa cttaaaaaag gcgatgacca tcgccagcat caggagggtc gccggaagga
    29521 ccgtcccggt cacggccatg accgcgcccg gaaagccggc catcttgtat ccgatgaagg
    29581 ccgaaacctg cggggcgatc gggcctggaa gagcgttgcc cagggcgacc gcgtcggcga
    29641 actccccgga cgtcacccaa ccggcggacg tcgcctccct ctgcatgaga gccagcgagg
    29701 ccggtccgcc tccccaggaa aacagggcga cccgggaaaa gacggtgaaa agcttccata
    29761 gcattgtcct cgactcctcc gcggggcgcc ctctgaaggc cgccccgccc cgccgcatca
    29821 tcgtaaggtg tccagcggcc tcttgtcaaa accggctgcg agaaacgacc tcccgtttcg
    29881 acgcgacgcc ccgcgatctc ctctttttgg ggcgaggatt cttcccacgg cggaggaaaa
    29941 gttctttaga aagcttccgc ctggctcaaa tccgggtccc tgtgttaaga tgcgcgggga
    30001 atttaagccc gcaggagtct tgcatgaccg gcctttccgt cctgatcgcc cttcttccgg
    30061 tgatcctcat cctcgttctt ctcatcgggc gcaagaccgc cgccgacacc gccgggctgg
    30121 cgggctgggt cgcggccgcg gccgcggccg gattatattt ccgaacgccg ctctcgatca
    30181 tcctcaagtc cagcctcggc ggcgtgctcg cctcgcttcc gatcacgatc atggtcggga
    30241 cgtcgattta ccagatcacc gtcatgctcg agaccggggc catctcccgg gtcgtcgccc
    30301 tgatcaagac cctggccccc cgggacaaga tcatccagat catgatcatc aacgtcggct
    30361 tcggaacgct tttggcggcc atgggggcga cgccggtttc gattcttccc ccgatcatgc
    30421 tcggcctggg ctattcctcg ttcgtggcca tcgcccttcc cgccctcgga tacgacgcct
    30481 tgtgcacata cgcccttctc ggcgtgccgg tcgtcgtctt cgccaatttc gtcggccttc
    30541 cggtggagca ggtcggcggc tatttcgccc gcttcatgcc cgtcatcacg tcctgcatcg
    30601 ccctggggat gctctggatc gccggcggcc tcaagacgct cctcaagggc gtctttcccg
    30661 ccgtcctggc cggcgtaacg gccggaatga tcgccatcgg catgaaccaa gcccgcatgg
    30721 tcaccctgac cggggtggcc gccggcctcg gcgtcgtcct ggtcatgttc ctctatctcc
    30781 ttattgcccg gaaaccgatc cgggaccgga gtgtgctgac ggacgccgac cgggcctccg
    30841 aacaacggat gtccctcggc cgggccgttt cgccttggat catcctgacc gttttcgccg
    30901 tcctgatcaa cacgccgtcg ctgccctttt tccgcctggt cttcgaccgc tgggccatgc
    30961 ccgtggagat cattcccggc gcccccgaaa agctgcggct cctctggcag gcgtatttct
    31021 ggattcttgt cagcacgctc ctggccctgc cgttcctcaa gccctccaag gcgcagcttt
    31081 ccgcttgcgg gagaaaatgg ctgaagcggg ccccccggcc gatgctggcc tcggccgtct
    31141 ttttcgccat cgcctacatc atcaatcatt cgggaaaagg gccggactgg tcgctggccg
    31201 agccggggca taacatggtc tacgtcctgg ccgccgcctc cgcccaggcc ttcgaccggc
    31261 tctatccggc cgtggcctcg ttcctcgggc tgctcggagg cttcctcagc ggatcggaga
    31321 cctcggccat cgccatgctg accaagcttc atctctcgac cgctgaaaag atcggggccg
    31381 ccggcgtcgt catcgccgcg gccagcggga tcggcggcgg gctggccagc gtcatctccc
    31441 cggccaagct ccagaacgcg gccgccagca tcgaccgcat cggcgaagag ggccgggtcc
    31501 tccggacgac gtttgtgatc tccctcttca tcacagccgt ctgcgccgcg atgagcctcc
    31561 tgtgggcgtt ttaagggatc cggttgttga aatcgggccg gacggcgggg ttcgagggaa
    31621 gctcgcgttg aacgcccggg ggacctgtga tacaaaaagg cccatgttcc gcaagcccgc
    31681 cgccgtcttc attgtctttt tattagcctc cctcctccct cccctcgccg ccggggaagc
    31741 ggacgaaaaa gagtccggga cgccgcggtg gatcttctcc ttcgccgagc ggatccgcca
    31801 ggaaagctcc gacaacgtcg cttccctgga tgaatccaaa accgattcct cctcctatat
    31861 gaggatgagg acgaatctgg cggtctcatg gaagccggcg gaaacgctcg ccttcgggct
    31921 gcgcctggcc aacgagataa gatattacct gtcccccaag accgacccga agctggggat
    31981 caatttcggc ctgaatgaag tcgtcatcga tcagctctac gcccgctggg accgcccggc
    32041 cgggctgccc ctcgccctga ccgtcggccg ccaggacatc cagatgggcg agggattcgt
    32101 cctgatggac ggcggcccgg gcgacggctc ccggacggcg tatttcaacg gggcgcgggc
    32161 ggatgtgacc ctcgcctccg gccaaacgct caccgcgttc tacgtcaacc agccgcgcac
    32221 cgatcgattt cttccccgcc tcaacgacgt cggccagatc atggtcgaac aggacgagga
    32281 aagctacggg ctctatcaca ccggcggctt cggaaaaacg aaattcgagg cctatttgtt
    32341 ccggctcaac cgggaacgcc gcggcctgtt ccccggcgcg ggcttccaga cggcgggagg
    32401 gcgcgtccaa gcccccttgg ccggccgtct ttccctgacg gccgagggcg cgctccaatt
    32461 cggccgccgg gaagacgatc ggaaaaccgg cttcggggga tacgcgcgtt tagattacgc
    32521 gaccggcgcg gccggggcgc ttccggcgac gctcaccttg ggcgggattt ggctcagcgg
    32581 cgacgatccg gc
//
LOCUS       DAOOXH010000018        31685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_1204_length_31685_cov_7.521467, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000018 DAOOXH010000000
VERSION     DAOOXH010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 31685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31685
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1204_length_31685_cov_7.521467"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PKV64_03780"
     CDS             <1..507
                     /locus_tag="PKV64_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007462595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPN16235.1"
                     /translation="ELGVADQVKFIHGDATGYVSEEKAGVAACVGATWIGGGVAGTIE
                     LLARSLRPGGIILIGEPYWRKRPPTEDVAKGCLAGSISDFLPLPELLASFGRLGYDVV
                     EMVLADQDSWDRYEAAKWLTMRRWLEANPDDELANDVRAQLTSEPGRYAAYTREYLGW
                     GVFALMAR"
     gene            complement(573..917)
                     /locus_tag="PKV64_03785"
     CDS             complement(573..917)
                     /locus_tag="PKV64_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16236.1"
                     /translation="MAENDVKKPKRELDPENSGRILSASAVLIGVCLTAIGLIRITIS
                     LQRKNTIADDLLALDAVLFLASFFLSYWALRYRDVPRPRKLERAADIVFMIGLVFLVA
                     IGVYIVYMISAP"
     gene            complement(929..1609)
                     /locus_tag="PKV64_03790"
     CDS             complement(929..1609)
                     /locus_tag="PKV64_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPN16237.1"
                     /translation="MSREKKQESIPTSGGDLRITFLGHASLVFDWKDILVYADPVSTE
                     ADFAALPKADVVLVTHGHFDHLDPKAVALLSKPGTEIFCDPESRPRLSRARALKNGEA
                     ASSRGLGIQAVPAYNITGMRSEGVPFHPRGTGNGYILTFGDVRVYVAGDTENTPEMKA
                     LKNIDVAFLPMMRPYTMSPDMIADAVRAMRPKILYPYHTKDADAEPLRRLLRDVPEIE
                     VRFRYGGN"
     gene            1729..3459
                     /locus_tag="PKV64_03795"
     CDS             1729..3459
                     /locus_tag="PKV64_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN16238.1"
                     /translation="MLKRSLFLKIFGGFGLMILLMSVLTTFISFGIIRSHYHRRLATE
                     LEHVGRAMTLDILRLMEGPPDALESFLVDEGRKIESRVTVIAPGGAVLGDSERNPAEM
                     ESHRYRPEVAEALEGKTSRADRFSYTVEDRMIYVAIPLKDDSGGVRAVLRLSVYARGV
                     DALLADLRGGIIRAVLIVTALALLAALFFTLSLTRPLQALVRASEKVAEGDFGAKVRV
                     GGGGEFRHLAGVFNAMTDRLEAQFEDLERRKEELANIIASIREGLAVIGRDGRILFSN
                     EAFHSLFPKAAGGNRGYLETVRSAGLQSLIEGSRREKAGRTVDVKIGDRSFLAAAGYL
                     PAQDGIVLLLTDMTERRRVDEMKKDFIVNASHELRTPLAAISGALELLLDGKAINDPA
                     ALDILSRHVVRMRAIVDDLLKLGELESPETRLDLKAVDPAALARRVVELYADRAAEKG
                     LFLKIEAAPDLPPVRADEVLLEQLLINLVDNAVKYTDRGGVTISLSPGKGQMTFQVSD
                     TGPGIAAEHLPRIFERFYLVDPARSRKLGGTGLGLSIAKHIVLLHGGAIEARSEAGAG
                     TAFSVRLPLA"
     gene            3524..5221
                     /locus_tag="PKV64_03800"
     CDS             3524..5221
                     /locus_tag="PKV64_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPN16239.1"
                     /translation="MLSFAERLTLDDIRRGILGRDLAFPTPFGPRHLFYADYTASGRL
                     LDFVEKKLLEISRSYANTHTEDDYTGKTMTRRFKEAERRIKASVNAGPRDKIFPTGSG
                     STGALKKLQEMLGVYLPPVTRERIEALCRTAAARGVDLGPEVKKITPVVFIGPYEHHT
                     NELMWREAFAEVVVVGLDEAGGLDLRDLERKLDDPAFAGRIRFGSFSAGSNITGVRTP
                     VYDVARLCRCRGVPVFFDFAAIAPYVEIDMNRDEESAFDAVFFSPHKFLGGPGSCGIL
                     IMKDRLYRPDLPPTTAGGGTVVYVGPKSHDYAVDIETRETAGTPPILQTIKAALAVEV
                     KEAAGAARIERIERGHLARFMRGLGRIPGIRTVGPGGVENRTPIVSFNISHRDRILHP
                     KFVTKLLNDLFGIQSRAGCSCAGPYGHRLLGIGDNDSIRYRDAILCGREGLKPGWVRI
                     NLHWVFEREDVDFLLRAIDFTARNGEQFLRLYGFREETGEWIHVDESGRAGSPGDSAP
                     PAGKKIPAARIPALRESYFRDAEAAAAGLRRLGPPPWKPDGPEGSLRWFYEADIGSD"
     gene            5284..6672
                     /locus_tag="PKV64_03805"
     CDS             5284..6672
                     /locus_tag="PKV64_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPN16240.1"
                     /translation="MVPAGKSRPERPSGSPTDAVLESISDGVFTVDREWRITSFNRAA
                     EEITGIPRKEALGRFCSDVFRSSMCEADCALRRTMKTGKPIIGRSGFIIKADGRRIPI
                     SVSTAVFRDRTGRTAGGAETFRDLSEVEALRRELDGKFRIGEMASHSPLMQRIFDVLP
                     AIAASPSTVLILGETGTGKELAARTIHELSPRRNGPFVAVNCGALPDTLLESEIFGYK
                     AGAFTGAVKDKPGRLALAEGGTLFLDEIGEVSPAFQVRLLRVLQERTYEPLGATRPKS
                     ADIRVLAASNKDLAALVKEGRFREDLFYRVNVVRLELPPLRQRKEDIPLLAAQFIERF
                     NRLQGKSVKGVDGEALSLLMARDWPGNIRELENVIERAFIVCGDGPIGVAHLPEDLIP
                     DAGRSDVRASHDALDAQAIRAAIENAGGNREAAARDLGIHKTTLFRRIKRLGLSFPGP
                     DGRSRRRPPDRS"
     gene            6709..6993
                     /locus_tag="PKV64_03810"
     CDS             6709..6993
                     /locus_tag="PKV64_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16241.1"
                     /translation="MRVSARVQRSLRIGVLLAFTILSLGVHFFHTEKGIYEPAGCPAC
                     HFLVFSQSVGPVLVFLLPLLVLLGAVCLEISIRLNQTGIRLYLSRSPPSV"
     gene            7057..8325
                     /locus_tag="PKV64_03815"
     CDS             7057..8325
                     /locus_tag="PKV64_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPN16242.1"
                     /translation="MARLRGLFPIILIIGLGAVSGFSGPNDLPDGLTISQCIEISLSQ
                     NPLWLSSEHDVRASLARIERAKARPRPNLDFDSDLQPGFLDFGGSGESYLGVSQILEF
                     PGRRGLRTRIARLESEEAAADRCVLRAEIVFQIKETFFGVLLAEEKRNYAARNLALAR
                     DFLEKAKVKFEAGDAARVEYVRAGVEESKAATALAVAENRILLEKARLNFLLGRPKSE
                     PLNLRGSLIRARVPFDVVSLVERAQAVRPEMAAMNARLAKAGAAKKEAGLSYLPDFSL
                     GLSRHRIAGEKTTWDFTLSVPVPLFFWQPLRGEIAERQAELLSLEQKARQMRNSISLE
                     VEQAGFQARAAEGQIALFQDKILVQAREVHDLFLYSFQEGEITGIELIEARRTLIEAL
                     ESYADALFVYNTALASLEKSVGAPIEGVEN"
     gene            8322..9596
                     /locus_tag="PKV64_03820"
     CDS             8322..9596
                     /locus_tag="PKV64_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPN16243.1"
                     /translation="MNKNIPAVVCLFLLILGSSACSRPAEDAGRGRKAPGAEIPGRGF
                     GPGRGADVRGVRHGLERGGGAARLRAAAESIRLTEAETGAVEIETEPVSFRPMVDKLQ
                     ATGKIQAHQFGKAIISYPFPARISQIHVRPGDWVNPGQSLVTLQSEAVGETKAEYFKA
                     LADLDLAVNNLEREKRLFERGAGAGKNLQAAEAEWKVAEAARNAAEKKLHILGFTEEQ
                     VRAAGAVHEINPLITLFAPIAGNVVDNNAVLGGMVDQATEILTILDPRRLCVDADIYE
                     RDIARVRLGQRVDVSVPAYPDLVFKGTLKYVGDVLKEETRTVTVRTEVENEGRRLKPG
                     MFATLDIVLEENSRALTVPVAAVLDDGLEEIVFIRKDGEFLRRVVRTRSKVDGYLEVT
                     DGLSEGEDVVVKGNFQLKSKLYDEILKAAGIH"
     gene            9637..12741
                     /locus_tag="PKV64_03825"
     CDS             9637..12741
                     /locus_tag="PKV64_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HPN16244.1"
                     /translation="MINKLIEFALNHRLLTVFLIGLVCLWGIVVYFRMPKDIYPELNA
                     PLVKIITENPGMASEDVERLITFPLESLLSGAPGVTRVRSESTTGDSVVTVEFEWGED
                     IYKARQIVDSKLELITGRLPQGTRTPTLGPVSSRMGEVFEFAVIGEPGTDPMELRSTA
                     DWTIRYQLQGVPGVAFVINLGGFVKQFQVFLKPDMLKNYGITVADVKAAIESSNRNFS
                     GGSIVKGSQEILVKGLGQIETLEHIRNTVLSSVRNVPVFVKDVADVRIGGKFRREAAG
                     HDGEEAVYVTVEKQYGGDTLTAIANIKGALGRISRDLPKYIGIKPFYDQSVLILKSIQ
                     HVEITILEGVLLIILVMIFFMWNLRSALLASLTIPFSILIALVIMDLFGIKLTVMSIG
                     GLAIGIGKMANGSIIMIENIFRLLKERAGRAPLRDIVAEAAKDVGKYLFSASLVIILV
                     FLPLLTLQGLEGAMFRPTAFAVAAALFAAMLLNITLQPVLAVVFLSRGKIKDAKDPVT
                     DFLAAKYKLVMEKALSKRTIVLVLFFVLIAGAAVGFLFLGKEFVPPLDEGAIMASTVM
                     LPETSLEESVKMGRRIERIFLSFPEVVSVSRTTGTAEASEHLHPVNHSHYNIELVPRE
                     KRKRGFDELAAAMRDELEKLPGVAFIFEQPIANKLAEMMTGTEGQLAIKLFGPDLAVL
                     NAKIEEIRNVLADVPGAADLQVEQTTGVPQLVIRLDREKLARFGIPVADVAGLIETAL
                     NGIEVTDVYEADRVTPVLIRLSEEFRKDEDAVRNLLIDAPGGLRIPLSELAELRRDEG
                     PQTIFRENLMRRKIIMGNVEGRDIGGFVKEARDKIEDGVDLPPGYYVTFGGQFESQQR
                     ALRHLAFLMMLVILIIFVILFSSFGSLRQALLVIMNVPSALIGGIAGLLIAGQTLNVS
                     STIGLIALFGICAQNDIILVAKINDLRRKGRALREAVVEGALMKFRPIFMTDMVMIVA
                     LLPMALVNATGSELHRPLAVVYIGGFFCAIFLRLIMVPVLYETLERIGVKKKRDKEGM
                     ESSG"
     gene            12913..13494
                     /locus_tag="PKV64_03830"
     CDS             12913..13494
                     /locus_tag="PKV64_03830"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPN16245.1"
                     /translation="MKMIAAFAAWNDRLAPVFDVTENVLILEIENGRIVRETRRALAG
                     EQPEEKIRALIQTGAEILVCGAISRYMHDRLAAAGIRVVPFIAGKLTEIIPAWIRGDF
                     DRTLFAMPGCGGGKGRQCRNGYFPEGGAADRGRGQGRRGTADPGRSRSGGGMGRGGGA
                     CVCPQCGLTEPHEYGVRCIERTCPKCGAVMARQ"
     gene            13535..13921
                     /locus_tag="PKV64_03835"
     CDS             13535..13921
                     /locus_tag="PKV64_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HPN16246.1"
                     /translation="MPKGDRTGIFGTGPMTGRAAGFCSGSGVPGFMNPSFGGGRGLGF
                     GRGRGFWSRGFGGGGFGRRNRYFAAGLPAWGRPGSYASPFVRRDPEWEKQTLREQAEA
                     ARAEIQEIEKRLGELEAEDEGGTRKS"
     gene            13987..14865
                     /gene="dapA"
                     /locus_tag="PKV64_03840"
     CDS             13987..14865
                     /gene="dapA"
                     /locus_tag="PKV64_03840"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPN16247.1"
                     /translation="MTKGVFTLQVTPFTKDLKLDEPGLRILIHRQIESGVHGIAPLGV
                     TGENPALTEAEVDRVVEIAVDEAGGKAKVAPDTCSNNLEQTIDRARRYAKIGADYVVV
                     FAPFLVLPTQAGLIDFYLRTADAVPVPIIIHNAPARVGVNLEHKGYQRLMEHGNIAGT
                     KDGKKELDHLAKLLHMARDKEFEVFTGKDTTAYPMMAFGGSGMFSVAANVVPGVMRGI
                     ADLCLAGKLEEARAIHYAYYDLFAALRLETNPMAAKAALSLMGLPGGPLRPPLTGLND
                     PNRETVRSLLAEKGMI"
     gene            14868..15227
                     /locus_tag="PKV64_03845"
     CDS             14868..15227
                     /locus_tag="PKV64_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPN16248.1"
                     /translation="MKIALTTSGKSLDALVDSRFGRAPGFLIYDLETKTFSVIDNEQN
                     QNASQGAGIQAAETIVRSGVRALVTGHCGPKAFRVLSAAGIAVYTADGLTVAETLSQY
                     VDGTLAEASAADVDGHW"
     gene            15237..16001
                     /locus_tag="PKV64_03850"
     CDS             15237..16001
                     /locus_tag="PKV64_03850"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HPN16249.1"
                     /translation="MKNRNNVNVFIDVLLFLVLAAVAGIGWLLKFKLLPGRERILKFG
                     ENRELLFLGWDRHQWGTVHLAAALAMLVLLVLHIVFHWKAIRCLLRNAVPSSPLRWTL
                     TGIAVGLAAVFFLGAFVIEPTKGELDIGFHRNIGPNAGRPDYDRTLAAEPPSPAAKGN
                     IEAASGEELGEKLAEDPLPAAADEKDHRPGSGNASLNGRMTLDEAAAACGISLQEARR
                     RLGLPEDYPGSETLGRLRRAWGTTMIRIRELLENPS"
     gene            16247..17119
                     /locus_tag="PKV64_03855"
     CDS             16247..17119
                     /locus_tag="PKV64_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HPN16250.1"
                     /translation="MKTSLDCLPCFVRQTLDAARMVSTDPAFHESFLREILARITEMS
                     LDTAPAVFGRELHRRIRGLSGRGDPYREAKERQNLFALALMPGLREQISAAPDPLILA
                     ARLAAAGNVIDLGAPGAISETSVRESIAQAMIRPLVGEENGFREEILSAENILYLADN
                     AGEIVMDRLFIERLGPSRVTVAVRGGPILNDATRADARAAGLAEIVEIIDNGSDAPGT
                     LLEDCSPEFLRRFEAADLVLAKGQGNFETLAGHPKNIFFLFKVKCPVIAAEAGAPVGA
                     HVLIRSAGAQGRLA"
     gene            17310..18167
                     /locus_tag="PKV64_03860"
     CDS             17310..18167
                     /locus_tag="PKV64_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN16251.1"
                     /translation="MILAIASGKGGTGKTTVAVNLARVLRSRAWLLDCDVEEPNAGLF
                     LKTSRRTEEIVSLPVPEVDEARCDGCGECGKFCAYHAIVSFGTIPLVFPSMCHGCGGC
                     VRVCPRKAIRETSRRLGVVVSSKSRGITLIEGRLDVGESLSPPLIRAVKAGLPAGRPA
                     ILDAPPGTSCPVVAAVRGADHVLLVTEPTPFGLHDLRLAVDLVRELGLPYSVVVNRIG
                     TGDNRVQEYCAGKKIPVVLEIPDDRRIAEAYSRGRLIVETLPEYRALFERLAEKLFSA
                     DGERCGLEP"
     gene            18290..19297
                     /locus_tag="PKV64_03865"
     CDS             18290..19297
                     /locus_tag="PKV64_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPN16252.1"
                     /translation="MEELVVISGKGGTGKTSLTASFAVLAGRPVIADCDVDAADLHLV
                     LSPRVRERHEFRSGREAVIRPEACTGCGLCSTLCRFGAVLSASSEPDVSPGAADCRDC
                     GYCVRSCPVRTNAVIREMRNASGGEVPSLYAIDPVACEGCGVCVRFCPEQAVDFPETV
                     GGEWMISETRCGPLVHARLAPAAENSGKLVSTVRREARRLAGEQGRGLILVDGPPGIG
                     CPVIASVTGASRILIVTEPTVSGEHDLERVLSLAGHFGIPASVCINKWDLNPETSDRI
                     EAAARRKGARLAGRIRYDPAFTRAQVREKAVVEIDAPGAGDIRNVWDRLELMKPGDKH
                     G"
     gene            19290..19661
                     /locus_tag="PKV64_03870"
     CDS             19290..19661
                     /locus_tag="PKV64_03870"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HPN16253.1"
                     /translation="MDESKSEVVSSIALDHAMHPRRLGRAKDADGRARITGPRGEITP
                     ILRGGAGKRIADDMGVPFCGSIPIDPKIAEAGDEGSVYVRHFAGSPAAGIMREIVRTI
                     FDRRGRRPEAGEPAASNGRKI"
     gene            19697..20035
                     /locus_tag="PKV64_03875"
     CDS             19697..20035
                     /locus_tag="PKV64_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPN16254.1"
                     /translation="MKIAIPMANGRLSAHFGHCEKFALVEVDPKEKKIVGRTDIDAPP
                     HEPGLLPGWLAEQGAEVIIAGGMGGRAMSLFAERGIKVIVGAPVETPERLAGDFLAGT
                     LSPGDNLCDH"
     gene            20094..20267
                     /locus_tag="PKV64_03880"
     CDS             20094..20267
                     /locus_tag="PKV64_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16255.1"
                     /translation="MLRADRERLISTAAALREINPGLVVPCHCTGEKAIEFLRTELPG
                     RVEAGRAGMALRF"
     gene            20342..20662
                     /locus_tag="PKV64_03885"
     CDS             20342..20662
                     /locus_tag="PKV64_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HPN16256.1"
                     /translation="MSTLEIDGKAVVLNEEGFLENPEEWDKRVALALAKREEGLDELG
                     PEHWAVIEYIRGYYVEKQLAPMVRKICQTTGLTLKHIYELFPSGPAKGACKLAGLPKP
                     DGCV"
     gene            complement(20796..22118)
                     /locus_tag="PKV64_03890"
     CDS             complement(20796..22118)
                     /locus_tag="PKV64_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN16257.1"
                     /translation="MTAVTKSPRNKLFFMMVLEFFIWGAWLPLIYSYLPSLGFTPVEQ
                     AWILNAFPIAAIIGMFFSNQFADRHFAAEKFLGFSHLIGGLAMLGLGFTRAFWPFLIL
                     MWIHCLFYVPTNSIANSIAFANMKDPQKEFGLIRMGGTLGWILAAWPFTFILVDWARV
                     KAAAPKGMVAWIGTVLGSGLTGQALKQATRWTFIVAAVASLALAAFSLLLPHTPPKKK
                     AAGAANGTERFAWLEAFKLLGRPFVLILWLIAFIDAFVHNLYFNWTGTFLGAAPAAGG
                     VGIAGNWIMPVMSLGQVSELLTMLILGVVLKKLGWRITMLVGILGHAARFAVYAFFPS
                     QPWMIITVQILHGICYAFFFATVYIFVDTYFPKDVRSSAQGLFNVMILGLGVLAANSL
                     GPWLLQSVFTTAGVTDYKSMFLLPCLIALGAAIFLAAAFRPPAAPAKD"
     gene            complement(22273..23166)
                     /locus_tag="PKV64_03895"
     CDS             complement(22273..23166)
                     /locus_tag="PKV64_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16258.1"
                     /translation="MDIETPLPILPVSVILIGFALPGFGGGAWQIRISVGPGFISPAD
                     LNSFLRDSIRIAEADSRSTARGLGFKPVRKAANFEAALSVPIEPRVQILAAYGMIKAE
                     TTGNEFTVTYPAVDKINLREDKIRDDYGRLGLAYEIPIADWISFRPYAAGELHRTSFE
                     DAGSWTNVSLKTGDRVLWMDWKLKTTAIKPGFSAGLGIEIAPAPVLRLWADAGYRRAK
                     ITGFKGDFTYFWGYPEGGYWEESSDVPLYYYEFDDSGEGPVYASLSMPGLWAGHRLTK
                     IRDAAVDLSGFYLKAGLGIAF"
     gene            complement(23353..24519)
                     /locus_tag="PKV64_03900"
     CDS             complement(23353..24519)
                     /locus_tag="PKV64_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPN16259.1"
                     /translation="MSEFIFLDNGATSYPKPDEVYMFMDKLYRKAGVNPGRSGYDLCL
                     EAGEVVDNTRKQMTRFFHGTDPNRLCFGYNSTDALNLALWGLLGEGDHAVTTMVEHNS
                     VLRPLYHLARDRKVELDIVPFDDRGFVDPDEIEKRFKPNTRVVAVNHASNVIGTIQPI
                     REIGRRCRARGIFFVIDASQSAGKIPIDLEADFIDVVCFTGHKSLLGPMGIGGLYVRE
                     GVEIKQTRAGGTGVRSAQKLHLEEYPYRLEYGTPNLPGIAGLHAGVKWVEARGVEALH
                     HQEMALAEKLAAGLRNIPRVKLYCLDDLRDHISVLAFNIEGMDAADVGTMLDVDHNIA
                     CRTGLHCAPLVHERLGTDKIHGAVRFGIGPFNTEAHIEAAIAAVADIAAAAKKR"
     gene            24714..25415
                     /locus_tag="PKV64_03905"
     CDS             24714..25415
                     /locus_tag="PKV64_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16260.1"
                     /translation="MSPLLQFVEDRLQIAALLFMASVYVIRVLWLFHFRAVRERAAAE
                     GRASAGAGYSLSNIARPWAMESTRTRPLFYLQFVVFHLGVTAAIAATFIIPYAPQWLA
                     PRGRVIAFQALMAAAFVVAVFRLVRRLRRPVLRLVSTPDDYAAITLMMAYFILGILAV
                     PNRPDRGEGPMIAFFILTAFFLVVVPFSKISHYLYYPFTRIILGRTLGRRGVLPVRRP
                     RSRQTAEERGEEVGA"
     gene            25412..26827
                     /locus_tag="PKV64_03910"
     CDS             25412..26827
                     /locus_tag="PKV64_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPN16261.1"
                     /translation="MKTVKAAKSLSVENLPLDVATQDKARADALPSIQKDMLRTYDPA
                     ADNVRRFLDLLAERITRPVAASLAACVHCGLCVESCHYALTHPDDPTMTPVYKADQIR
                     KIFKRRIDWTGRIFPWWVKAGTPRGDEDLNRLKNIVFGTCSACRRCTLNCPMGVDTAI
                     LIRFTRGLLTELGIVPEGVFNVSRDEWETGNQMAVTPLDFLETLQWMRDDLRADPKLA
                     GLDIPVDLPGCDFMYTINPREIKFDPRSIAQAAKIFHLAGEKWTLPSAGWDQTNFGLF
                     SGDDRLGGALARNLYEAAIRLRAGRIVISECGHGYRSTRWEGYNWAQYDQDLPSESVV
                     RTLQRYLREKRIRVDKTKNDLPVTFHDSCNVARSGGMTEEPRDVLRRVCADFREMTPN
                     RTENYCCTGGGGLLSMAEYRPLRMEAAAIKARQLEATGAKLVCTICHNCVDGLNDVIK
                     HYKLDMRVVQVLELVENALVY"
     gene            26841..27284
                     /locus_tag="PKV64_03915"
     CDS             26841..27284
                     /locus_tag="PKV64_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HPN16262.1"
                     /translation="MTKIGIIICGRYKTCGGGKCLRALRERVGAFARYPKNEAVEIVG
                     YSYCGDCPGGNVEYVPEEMKKNGAEVIHLATGLVVGYPPCPNIRRFKELIETKFEIPV
                     VIGTHPIPLKYAQAHEKLSFWKSAGMEALAGGLMREDKKIQEAYN"
     gene            27296..27781
                     /gene="folK"
                     /locus_tag="PKV64_03920"
     CDS             27296..27781
                     /gene="folK"
                     /locus_tag="PKV64_03920"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPN16263.1"
                     /translation="MRYYLGLGGNTGDRFANLEAAVRRLREEGVRIIGRSSIYRTEPV
                     GLRNQPWFLNQALKVETRLSPEQLLKTAKAVEAGMGRKPGRRNGPRPIDIDLLLAGKR
                     NVRTAGLQIPHPRLAERRFVLVPLAEIAPRAVHPVLRKTIRSILRDCPDASAVRRLRR
                     D"
     gene            27848..30058
                     /locus_tag="PKV64_03925"
     CDS             27848..30058
                     /locus_tag="PKV64_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN16264.1"
                     /translation="MKRMIVLIVTFIFIAPVFGADVVAVHGTVASTGGKPVSGAVVLQ
                     RESGLKTTTGEDGSFSLELERSGRTVLEVIHPDYSDGLFLLPAKSLDRPVRLTLAPLI
                     QQNEEVVVTALRYPEPTARIPAAATVLSSVSLGDAPAANLTGALERAIGVAPLGTGGF
                     SMVPTIRGMARNRILILIDNARIASDRRTGPSASFVSVEDIDRVEVLRSPSSIFYGSD
                     AIGGVIHIFTKSGREEGIHGTVHAGYGTNGAHAEYGLALSGKSGPFSFYLSGQNNKAG
                     TYDSPDGEVGQSQYGQTGFFGRAAYETEARRVDLSFLLGRGTDIGKPAKNTPTKPTWY
                     PRENQNLAQLHWIEKNVGGGELSVMAYANPNFLETRTDTITGAGGPGAYISKKAYAKT
                     ESTEYGAQLSFTKPLGGDFRLTGGADFFGRTNAGAINNETSYDASGNVKKILEETPYA
                     GGRRRDLGFYLSADYAGIRNVDLIAGLRFDTVRQSARPGGKPDELASNLNAATGFFGA
                     SMRLTDRWTIFANVSRAYRTPGLGELFYSGITGRGVIIAKPDLLPETSLNGDVGVRYV
                     GKRFFVGLYGFLYGIDDLIDRLLVAPGVYSYLNVEDARISGFELEAEWCPAAGWRIFG
                     QCAALDGRSRATGDPVNDIPPFRVQVGSRFLWRGLSLEVSGSYQAPKDRPGGAEIAIP
                     AYHYIQARLGYDFRSVGIFAVVKNALNAAYFGRPDPDGVYEPGRNILFGARYSF"
     gene            complement(30197..30646)
                     /locus_tag="PKV64_03930"
     CDS             complement(30197..30646)
                     /locus_tag="PKV64_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPN16265.1"
                     /translation="MGRGRPPPPRASRGRDRSGRHEGRNPLENRSKERIELGRRGEEA
                     AVLYLAGGRFRIVERGYRYGRGEIDIIARDGPALVFVEVKTRTDPRLGYPEEAVTPAK
                     RRQIRKIARSYLYEHPETAGLPCRFDVLAVEFQEDGRPIVRHIRDAF"
     gene            complement(30535..>31685)
                     /gene="cysS"
                     /locus_tag="PKV64_03935"
     CDS             complement(30535..>31685)
                     /gene="cysS"
                     /locus_tag="PKV64_03935"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPN16266.1"
                     /translation="IESFFAGLKTLRIHPADHYPRATEHIPDMVRIIKGLLDKGVAYR
                     KEGSIYFDISKFPSYGRLSGIDVEALRTTSRIGARLETDEYEKESARDFALWKAPKEG
                     EPFWETEIGPGRPGWHIECSAMSAAFLGPTFDIHCGGVDNIFPHHENEIAQSEAYFEK
                     PFVRYWLHCHHLIVDGEKMSKSKGNVYSLDDLVETRRVDPSVLRMFLISTHYRKVLNF
                     TFEALDQAAAALKRITDFVYELEHRAFPDAGSGLAGPLIAEADAEFRDGLGDDLNVSS
                     ALTALYDLIRKANSLIREEKIGADEARALRSFVSQVDDVLAVLPERREEEVSAAVREK
                     IEARELARKAKNWAEADRLRRELLEAGIVLEDTKDGIRWKIAPRKESS"
BASE COUNT         5873 a         9731 c        10305 g         5776 t
ORIGIN      
        1 gaactcgggg tcgccgatca agtcaagttc attcatggcg atgccaccgg ttacgtctct
       61 gaagaaaagg ccggcgtggc ggcctgcgtc ggagccacgt ggatcggcgg gggagtcgcc
      121 ggcaccatcg agcttttggc gcggagcctc cgccccgggg ggatcatcct catcggcgag
      181 ccctactggc ggaagcggcc gccgacggag gatgttgcca aggggtgtct tgccggctca
      241 atttccgatt ttctcccgct tccagaactt ctcgcgtctt ttggtcggct cggctacgac
      301 gtcgtggaaa tggttttggc ggaccaagac agctgggaca ggtacgaagc ggccaagtgg
      361 ctcaccatgc gccgatggct tgaagccaat cccgacgacg aattggcgaa tgatgttcga
      421 gcgcaactaa cctcggaacc ggggcgctat gccgcttaca cgcgtgaata cctgggatgg
      481 ggcgtgttcg cgctgatggc gcggtgaaga ggggattacc gggcgattcg gcaagcggtc
      541 ttcctcaacc cgctcccgcc cgcccgcgcc cttcagggcg ccgagatcat ataaacgatg
      601 tacacgccga ttgccaccag gaagaccaag ccgatcatga agacgatgtc ggcggcgcgc
      661 tccagcttgc ggggacgggg aacgtcccgg tagcgcaagg cccagtagga taaaaagaag
      721 gacgcgagga acagcacggc gtccaaggcg agcagatcgt cggcgatcgt gttcttgcgc
      781 tggaggctga tggtgatgcg gatcagcccg atcgccgtga ggcagacgcc gatgagcacc
      841 gcggaggcgg aaaggatccg gccgctgttt tcgggatcga gctctcgctt gggtttcttc
      901 acgtcgtttt cggccatggc gtttcctttc aattcccgcc gtaccggaac cggacctcga
      961 tctccgggac gtcccggaga aggcggcgga ggggctcggc gtcggcgtcc ttggtgtgat
     1021 agggatagag gatcttcggc cgcatcgccc ggacggcgtc ggcgatcatg tccggagaca
     1081 tcgtgtaggg ccgcatcatg gggagaaagg cgacgtcgat gttcttcagg gctttcatct
     1141 ccggcgtgtt ttcggtgtcg ccggcgacat acacccgaac gtccccgaac gtcaggatgt
     1201 agccgttgcc cgttccccgg ggatggaagg gaactccttc ggaccgcatg ccggtgatgt
     1261 tgtacgccgg gacggcctgg atcccgaggc cgcgggacga ggccgcttcg ccgtttttca
     1321 gggcccgggc ccgggaaagc ctcggccggc tttccgggtc gcaaaaaatc tcggttccgg
     1381 gcttgctcaa cagggcgact gccttgggat cgagatggtc gaagtggccg tgggtgacca
     1441 ggacgacatc ggccttgggc agggcggcga agtcggcctc cgtcgagacc ggatcggcgt
     1501 agaccaggat atccttccag tcgaaaacca gcgaggcatg ccccagaaac gtgatccgaa
     1561 gatccccgcc ggacgtcgga atcgactctt gttttttctc gcggctcatg gcggttcctc
     1621 ctcttttacc tttattcata gcaaaacccc cggcccggcg caaccggacg gatccggttg
     1681 cggggcggcc cgatttggga tataaaaaac tgtcgaacgg gaaatgacat gctcaaacga
     1741 agcctgtttc tgaagatctt cgggggattc gggctgatga ttctcctgat gagcgttctc
     1801 acgacgttta tttccttcgg aatcatccgt tcccattacc accggcggct ggcgacggag
     1861 ctggagcatg tcggccgggc gatgaccctg gatatcctcc gcttgatgga ggggccgccc
     1921 gacgccctcg aatcgttcct ggtggacgag gggcgcaaga tcgaatcccg ggtgacggtc
     1981 atcgcccccg gcggggccgt cctgggcgat tcggagagaa atcccgcgga gatggaaagc
     2041 caccggtacc gtccggaagt ggccgaggcc ctcgagggaa agacgagccg ggcggaccgg
     2101 ttcagctaca ccgtcgagga ccggatgatc tacgtcgcga tcccgttgaa ggacgactcg
     2161 ggcggggtcc gggccgttct ccggctcagc gtttacgccc ggggagtgga tgcgctcctg
     2221 gccgatctcc ggggcggcat catccgggcg gtcctgatcg tgacggccct ggccctcttg
     2281 gccgcccttt tcttcaccct cagcctgacc cggcctcttc aagccctcgt ccgcgcctcc
     2341 gaaaaggtcg ccgaggggga tttcggggcg aaagtccgag tcggaggggg cggcgaattc
     2401 cggcacttgg ccggcgtctt caacgcgatg acggaccggc tggaggccca gtttgaggac
     2461 ctggagcgcc gcaaggagga gctggcgaac atcatcgcct cgatccggga aggcctggcg
     2521 gtgatcggcc gggacggccg gattctcttt tccaacgagg cgttccattc cctcttccca
     2581 aaggcggccg gcgggaaccg cgggtacctg gaaacggtcc ggagcgccgg cctccaaagc
     2641 cttatcgagg gcagccggag ggaaaaggcc ggccggaccg tcgacgtcaa gatcggggac
     2701 cggagcttcc tcgcggccgc cggctatctt ccggcccaag acgggatcgt ccttcttctc
     2761 accgacatga ccgagcggcg gcgggtcgac gagatgaaaa aggatttcat cgtcaacgcc
     2821 tcccatgagc tgcggacgcc gctggccgcg atttccgggg cgctggagct tcttctggac
     2881 gggaaggcga tcaacgatcc ggccgctctg gacatcctct cccgccacgt cgtccggatg
     2941 cgggcgatcg tcgacgatct cctgaagctg ggggagctcg agtcgccgga gacccgtctc
     3001 gacctgaagg cggtggatcc ggccgctttg gcccggcgcg tcgtggaatt atacgcggac
     3061 cgggcggcgg agaaaggcct ctttctgaag atcgaggcgg ccccggacct gccgcccgtc
     3121 cgggccgacg aggttcttct ggaacagctg ctgatcaacc tggtcgacaa cgcggtgaaa
     3181 tacaccgacc gcggcggcgt gacgatctcc ctctcgcccg gcaagggaca gatgactttc
     3241 caggtctcgg acaccgggcc ggggatcgcc gccgagcacc tgccccggat tttcgagcgc
     3301 ttttatcttg tcgacccggc ccgctcccgg aagctcggcg gaacgggtct cgggctgtcc
     3361 atcgccaagc acatcgtcct ccttcacggg ggggcgatcg aggcgcggag cgaggccggg
     3421 gcgggaaccg ccttctctgt ccggcttccg ctcgcctgaa accgcggccg cgattggccg
     3481 gctttctttt tccggttaaa atgatcagga acgcgaggtg cccatgcttt ctttcgccga
     3541 acgcttgacc ttggacgata tccgccgcgg aatcctcggg cgcgacctgg cttttccgac
     3601 gcccttcggc ccgcggcatc tgttctacgc tgattacacc gcctcgggac ggctgctcga
     3661 tttcgtcgag aaaaagcttc tcgagatttc ccggtcctac gccaacactc acaccgagga
     3721 cgattacacg ggcaagacga tgacccggcg gttcaaggaa gcggagcgcc ggatcaaggc
     3781 ctcggtcaac gccggccccc gtgacaagat ctttccgacg ggctcgggct cgaccggcgc
     3841 cctgaagaag cttcaggaga tgctcggcgt atacctccct ccggtcaccc gggagcggat
     3901 cgaggccttg tgccggacgg cggccgcgag gggcgtcgac ttggggcccg aggtgaaaaa
     3961 gatcacgccg gtcgtcttca tcggccccta tgagcaccac acgaacgagc tgatgtggcg
     4021 ggaggccttc gccgaggtcg tcgtcgtcgg cttggacgaa gccggcggat tagatctccg
     4081 ggatctcgaa cggaagctcg acgatccggc cttcgccggg aggatccggt tcggctcgtt
     4141 ctcggccgga tcgaacatca ccggcgtccg gacgccggtc tatgacgtgg cccggctatg
     4201 ccgctgccgc ggcgtgccgg tctttttcga tttcgccgcg atcgcgccct atgtcgaaat
     4261 cgacatgaac cgggacgagg aatcggcctt cgacgccgtt ttcttctcgc cccataagtt
     4321 tctcgggggt ccgggaagct gcggcatcct gatcatgaag gaccgtctct accggccgga
     4381 tctgccgccg acgacggccg ggggaggaac ggtcgtctac gtcgggccga agagccacga
     4441 ctacgcggtt gacatcgaaa cgcgggagac ggcgggaacg ccgccgattc tccagacgat
     4501 caaggccgcc ctggccgtcg aggtgaagga ggcggcgggg gcggcccgaa tcgagcggat
     4561 cgagcgcggg catctcgccc gcttcatgcg ggggctcggc cggatccccg gcatccggac
     4621 ggtcggcccg gggggagtgg agaaccggac gccgatcgtg tcgttcaaca tctcccaccg
     4681 ggaccggatc ctccatccca agttcgtgac caagctcctc aacgacctct tcggcatcca
     4741 gtcgcgggcg ggatgcagtt gcgccgggcc ctacggccac cggctgctgg ggatcggcga
     4801 caacgactcg atccgctacc gggacgccat cctctgcggc cgcgagggcc tcaagccggg
     4861 ctgggtccgg atcaacctcc attgggtctt cgagcgggaa gacgtggatt tcctgctccg
     4921 ggcgatcgat tttacggccc gaaacggcga gcagtttctc cgtctctacg gctttcgcga
     4981 ggaaacgggg gagtggatcc acgtggacga atcgggccgg gcgggctcgc ccggagactc
     5041 cgccccgccg gccggaaaaa aaattccggc ggcccggatt ccggcccttc gggaatcgta
     5101 cttccgggac gccgaagcgg ccgcggccgg gctccggcgc ctcggcccgc cgccctggaa
     5161 gcccgacggc ccggaaggat ccctgcgctg gttctacgag gccgacatcg ggtcagattg
     5221 accttccgac ggtcaatcgc ctattgtaga atcgtgacca agacgaaaac acggcgatct
     5281 cggattgttc cggccgggaa atcccgtccg gaacgtccgt ccggttcgcc gaccgacgcc
     5341 gttctcgaaa gcatttccga cggcgtgttc accgtggacc gggagtggcg gatcacgtcc
     5401 ttcaaccgcg cggccgagga gatcaccggc attccccgga aagaggccct tggacgcttc
     5461 tgttcggacg tctttcgttc cagcatgtgc gaagccgatt gcgccttgcg gcggacgatg
     5521 aagacgggaa aaccgatcat cggccgctcc ggcttcatca tcaaggccga cggccgacgc
     5581 attccgatca gcgtttccac ggccgtcttc cgcgaccgga cgggacggac ggccggcggc
     5641 gcggaaacgt tccgggacct gagcgaggtg gaagcgttgc ggcgcgagct ggacgggaaa
     5701 ttccggatcg gcgaaatggc cagccatagc ccgctcatgc aacggatctt cgacgtcctt
     5761 ccggccatcg ccgccagccc gagcacggtc ttgatcctgg gggagacggg gacgggcaag
     5821 gagctcgccg cccggacgat tcatgaattg agccctcgcc ggaacggccc cttcgtggcc
     5881 gttaattgcg gggctctccc cgacaccctt ctggaatccg agattttcgg atacaaggcg
     5941 ggggcgttca ccggggcggt caaggataag cccggccgct tggccctggc ggagggcggc
     6001 acgctttttc tcgacgaaat cggggaagtg agcccggcct tccaggttcg tctcctccgg
     6061 gttctccagg aaaggacgta tgagcccttg ggcgcgacgc gcccaaaatc cgcggatatc
     6121 cgggtcttgg ccgcgagcaa caaggatttg gccgccctcg tgaaggaggg ccggttccgc
     6181 gaggatctct tttatcgcgt caacgtcgtc cgcctggagc ttcctcccct gcgccaacgc
     6241 aaagaagaca tcccgctgct tgcggcccaa ttcatcgaga ggttcaaccg ccttcaaggc
     6301 aaatccgtca agggggtgga cggggaggct ctctcgcttc tcatggccag ggactggccg
     6361 ggcaatatcc gcgagctcga aaacgtcatc gaacgggcgt tcatcgtctg cggtgacggc
     6421 ccgatcggcg tcgcccacct tcccgaggat ctgattcctg acgccgggag gtccgatgtt
     6481 cgggcctccc acgacgcgct cgacgctcaa gcgatcaggg cggcgatcga aaacgccggc
     6541 ggcaacaggg aagcggcggc ccgggatctc ggcatccaca aaaccaccct cttccgccgg
     6601 atcaagcggc tcgggctttc cttccccggc cccgacggcc gttcccgccg ccggccgccg
     6661 gaccgctctt gatttgtctc ccccggttat agtaatttaa aaaacgccat gcgggtttct
     6721 gcgcgggttc agcggagttt gcggatcggc gtcctgctgg ccttcacgat tttatcgctc
     6781 ggcgtccatt tctttcatac ggaaaaagga atctacgaac cggccggctg cccggcttgt
     6841 cattttctcg ttttcagcca gtccgtcggg ccggttctcg tgtttttact cccgttgctg
     6901 gttcttctgg gagccgtctg tcttgaaata tccatccggt tgaatcaaac cggaatccgt
     6961 ctctatcttt cccgttctcc gccatccgtc tgatccttcc tgccggcctt ttccctcgat
     7021 ttcctgacat cctcggtttg aacatgagga ggacgaatgg cgagactacg cggattattt
     7081 ccgatcattt tgatcatcgg gctgggcgcg gtttccggct tctcggggcc gaacgacctt
     7141 ccggacggat tgactatttc ccaatgcatc gagatttcct tgtcgcaaaa tcccctttgg
     7201 ctgtcctcgg agcacgacgt ccgggcgtcc ttggcccgga tcgagcgggc caaggccagg
     7261 ccccggccca atctcgactt cgattcggat cttcagccgg gctttctgga cttcgggggc
     7321 tcgggagaat cctacttggg cgtatcccag atcctggagt ttccgggccg gcggggattg
     7381 cggacgcgca tcgcccgctt ggagtcggaa gaagcggcgg cagaccggtg cgtcctgcgc
     7441 gccgagatcg tcttccagat caaggagact tttttcggcg tcctcctggc ggaagaaaaa
     7501 aggaattacg ccgcccggaa tctggccctg gcccgtgatt tcctggaaaa agccaaggtc
     7561 aagttcgaag ccggggacgc ggcccgggtg gagtatgtcc gggccggagt ggaggaatcc
     7621 aaggccgcca cggccttggc cgtcgccgaa aaccggatcc tcctggaaaa ggcccgcctc
     7681 aatttccttc tcgggcgccc gaaatccgag ccgctgaacc tccggggaag cctcatccgc
     7741 gcccgcgttc ctttcgacgt cgtttccctc gttgaacgag cccaggcggt ccgccccgag
     7801 atggccgcga tgaacgcccg gctggccaaa gccggggcgg cgaaaaaaga agccggcctt
     7861 tcctacctgc ccgacttcag tctcggcttg tcccggcacc ggatcgccgg cgagaagacg
     7921 acttgggatt tcaccctctc cgttcccgtg ccgttgtttt tctggcagcc gctccggggg
     7981 gagatcgccg aaaggcaggc ggagctgctg tccctggaac aaaaggcccg gcagatgagg
     8041 aattccatca gtctcgaggt cgaacaggcc gggttccagg cccgggcggc cgagggacag
     8101 atcgccctat tccaagacaa gattctggtc caagcccggg aagtgcatga tttgtttctc
     8161 tacagctttc aagaaggcga aatcaccggg attgagctca tcgaggcgcg gcggacgctg
     8221 atcgaggccc tcgagtccta tgccgacgcc ttgttcgtct acaacacggc cctcgcctcg
     8281 ctcgaaaaat cggtcggcgc gcccatcgaa ggagtcgaaa attgaataag aacatcccag
     8341 cggtcgtttg tctatttctt ctgattctcg gttcgtcggc gtgctctcgt ccggcggagg
     8401 atgccgggcg cgggcgaaag gcccccggcg cggaaatccc cggccgcggt ttcgggccgg
     8461 gacggggggc tgacgtccgg ggcgtccgtc acggactgga acgcggcggc ggcgccgccc
     8521 ggctccgggc ggcggcggaa tcgatccgcc tgaccgaagc cgagaccggg gccgttgaaa
     8581 tcgaaaccga accggtttcc ttccggccga tggtggacaa gctgcaggcc acgggaaaga
     8641 tccaggccca tcagttcggc aaggcgatca tcagttaccc gtttccggcc cgcatctctc
     8701 aaattcatgt ccggccgggg gattgggtga acccggggca atccctggtc actcttcaaa
     8761 gcgaggccgt cggcgagacg aaggccgaat atttcaaagc actggccgat ctcgacctgg
     8821 ccgtcaacaa cctcgaacgg gaaaagcgcc tcttcgagcg gggggccggg gccggcaaga
     8881 atctccaggc cgccgaagcg gaatggaaag tggccgaagc cgcccgcaac gcggctgaaa
     8941 agaagctcca tatattaggc ttcaccgagg agcaggtccg ggcggccggc gcggtccacg
     9001 aaatcaatcc gctgatcacg ctcttcgccc cgatcgccgg caacgtcgtc gacaacaacg
     9061 cggtcctggg cgggatggtc gatcaagcaa ccgagatctt gacgattctc gaccctcgcc
     9121 ggctctgcgt cgacgcggat atttacgagc gggacatcgc ccgggtgcgt ctcggccaga
     9181 gggtcgacgt ctcggttccg gcgtatccgg acttggtgtt caaggggacg ctcaaatacg
     9241 tgggggacgt cctgaaggag gaaacccgga cggtcacggt ccggaccgag gtcgaaaacg
     9301 aggggcggcg cctgaagccc ggcatgttcg cgaccctcga tatcgtcctc gaagaaaaca
     9361 gccgggcttt aaccgttccg gtcgcggccg tcctcgacga cggcctggaa gagatcgtct
     9421 tcatccgcaa agacggggaa tttttacgcc gggtcgtccg gacgcgatcc aaggtcgacg
     9481 gctatctcga agtcacggac gggctgtcgg agggcgagga cgtcgtggtc aagggaaact
     9541 tccagttgaa gtccaagctc tacgacgaga tcctcaaggc cgcggggatc cattgagagg
     9601 aacggccggt ccgtcttcgg ccgcggagga tccatcatga tcaacaagct cattgaattc
     9661 gccctcaatc accgtctctt gacggtgttc ttgatcggcc tggtctgtct gtgggggatc
     9721 gtcgtttatt tccggatgcc gaaagacatc tatcccgagc tcaacgcccc cctggtcaag
     9781 atcatcaccg aaaatccagg catggcttcg gaagacgtcg aacggctgat cacctttccc
     9841 ctggaaagcc ttctttcggg cgcgcccggc gtcacccggg tccgctcgga gtcgacgacg
     9901 ggggattccg tcgtcaccgt cgaattcgag tggggggagg acatctacaa ggcccgtcag
     9961 atcgtggaca gcaagctcga gctgatcacc ggccgtcttc cccagggaac gaggacgccc
    10021 acgctcggcc ccgtctcgtc ccgcatgggc gaggttttcg agttcgcggt catcggcgag
    10081 cctggaacgg acccgatgga gctccgctcg acggccgact ggacgatccg ctatcagctc
    10141 caaggcgttc ccggcgtcgc cttcgtcatc aatctcggcg gcttcgtcaa gcagttccag
    10201 gtctttttga agccggacat gctgaagaac tatggaatca ccgtggccga cgtcaaggcg
    10261 gccatcgaga gcagcaaccg gaatttcagc ggcggatcca tcgtcaaggg atcccaggag
    10321 atcctggtca aggggctggg acaaatcgag acgctggagc acatccggaa cacggttctc
    10381 agctcggtcc gcaacgtgcc cgtcttcgtc aaggacgtcg ccgacgtccg gatcggcggc
    10441 aagttccgcc gggaggcggc cggccacgac ggcgaggaag ccgtctacgt caccgtcgag
    10501 aagcaatacg gcggcgacac gttgacggcc atcgccaaca tcaagggcgc cctgggcagg
    10561 atcagccggg atctgccgaa atatatcggg atcaagccgt tctacgacca gtccgttctc
    10621 attctcaagt ccatccagca cgtcgagatc acgattctcg aaggcgttct cctcatcatc
    10681 ctggtcatga tcttcttcat gtggaacctg cggagcgccc tcttggcgtc cctgaccatt
    10741 cccttttcga tcctcatcgc cctggtgatc atggatctgt tcgggatcaa gctgacggtc
    10801 atgtcgatcg gcggcctggc catcgggatc ggaaaaatgg ccaacggctc gatcatcatg
    10861 atcgagaata tcttccggct tttgaaggaa cgggccggac gggcgccgct ccgggatatc
    10921 gtcgccgagg cggcgaagga cgtcggcaaa tacctgtttt cggccagcct cgtcatcatc
    10981 ctcgtttttc tccccctgct gaccttgcag gggctggaag gggcgatgtt ccggccgacc
    11041 gccttcgccg tggccgcggc cctgttcgcg gccatgttgc tgaacatcac cctccagccg
    11101 gtcttggccg tagtcttcct gtcccggggg aaaatcaagg acgccaagga cccggtgacg
    11161 gatttcctgg cggccaagta caagctcgtc atggaaaaag ccttgtccaa gcggacgatc
    11221 gtcctcgtcc tcttcttcgt cctcatcgcc ggggcggccg tcgggtttct cttcctcggc
    11281 aaggagttcg tccctccgct ggacgagggc gcgatcatgg cctcgacggt catgctgccc
    11341 gaaacgtcgc tggaggaatc ggtgaagatg ggccgaagga tcgagcggat cttcctgtcg
    11401 tttccggagg tggtttcggt ttcgaggacg acgggaacgg ccgaggcttc cgagcatctc
    11461 catccggtca atcacagcca ttacaacatc gagctcgtgc cgcgggaaaa gcgcaagcgc
    11521 ggattcgacg agctggccgc ggccatgcgc gacgagctgg aaaagctccc cggcgtcgcc
    11581 ttcatcttcg agcagccgat cgccaataag ctggccgaga tgatgaccgg aaccgaggga
    11641 cagttggcga tcaagctgtt cggccccgac ctggccgtcc tgaacgccaa gatcgaggaa
    11701 atccggaacg tcttggccga cgtcccgggc gccgccgacc ttcaagtcga gcaaacgacc
    11761 ggcgttcccc agctcgtcat ccgcctcgac cgggagaaac tggcccgttt cggcatcccg
    11821 gtggccgacg tcgccggtct catcgaaacc gccctcaacg gcatcgaggt caccgacgtg
    11881 tacgaggcgg accgggtgac tccggttctc atccgccttt cggaggagtt ccggaaagac
    11941 gaggacgccg tccgcaactt gctcatcgat gcgccgggag gccttcggat cccgctgtcg
    12001 gagctggccg agctccggag ggatgaaggg ccgcagacca tcttccggga aaacctcatg
    12061 cggcggaaaa tcatcatggg caacgtcgag ggccgggaca tcggggggtt cgtcaaggaa
    12121 gctcgggata aaatcgagga cggcgtcgac ctgccgcccg ggtattacgt cacgttcggc
    12181 gggcaattcg aaagccagca gcgggccctc cgccacctgg ccttcctcat gatgctggtc
    12241 atcctgatca ttttcgtgat cctgttttcc tcgttcggct cgctgcggca ggcgctcctg
    12301 gtcatcatga acgtgccctc ggccctgatc ggcggcatcg ccgggcttct catcgccggg
    12361 caaacgctta acgtctcttc aacgatcggg ctgatcgccc tgttcggcat ctgcgcccaa
    12421 aacgacatca tcctcgtggc caaaatcaac gacctgcggc ggaagggacg ggcgctgagg
    12481 gaagcggtcg tcgagggcgc cttgatgaag ttccggccga tcttcatgac ggatatggtc
    12541 atgatcgtcg ccctactgcc gatggccctc gtgaacgcga cgggatcgga gctccaccgg
    12601 ccgctggccg tcgtttacat cggcggcttt ttctgcgcca ttttcctccg gctcatcatg
    12661 gtcccggtgt tatatgaaac gctggagagg ataggcgtca agaaaaaacg ggacaaagaa
    12721 ggaatggaga gtagcggata agcaacgata gacgataggc acaggagcgc aagggatact
    12781 ataaaaagcg gggcgggatg gcctgtgacc ttgtaatgat catataaaga ataagatacg
    12841 ctgacaagtc ctcgccgggc gggattggca cgaaattcgc tctattcttt tcccttgaaa
    12901 cgcgagaaac gaatgaaaat gatcgcggcc ttcgccgcct ggaacgaccg gctcgccccg
    12961 gtcttcgatg tcacggaaaa cgttcttatt ctcgaaatcg agaatggacg gatcgtccgg
    13021 gagacgcgcc gcgccttggc gggagaacaa cccgaggaaa aaatccgggc gttgatccaa
    13081 acgggggccg aaatcctggt ctgcggggcg atctcgaggt atatgcacga ccgtctcgcc
    13141 gcggccggaa tccgggtcgt gccgttcatc gccggaaagc tgaccgagat catcccggcc
    13201 tggattcgag gagatttcga tcgaacgctt ttcgccatgc cgggttgcgg tggcggaaag
    13261 gggagacaat gccggaacgg atatttcccg gaggggggcg ccgcggacag aggacgggga
    13321 caggggaggc ggggaacggc ggacccgggc agaagccggt ccggcggagg gatgggccgc
    13381 ggcggcggcg cgtgcgtttg cccgcagtgc gggttgacgg agccccatga atacggcgtc
    13441 aggtgtatcg aacggacctg ccccaagtgc ggggccgtca tggccaggca atgaatgatt
    13501 tcgaaattca aattatgaca aggaggataa aaaaatgcca aaaggagaca gaacggggat
    13561 cttcggaacg gggccgatga ccggccgggc ggccgggttt tgctccgggt ccggagtgcc
    13621 cggcttcatg aatccctcct tcggcggggg aagggggttg ggtttcggac gaggccgcgg
    13681 tttttggagc cggggcttcg gcgggggcgg gttcggccgg cggaacagat atttcgccgc
    13741 gggtcttccg gcctggggcc ggcccggctc ctatgcctcc cccttcgtga gacgcgatcc
    13801 ggaatgggag aagcagacgc tcagggagca ggcggaagcc gcccgggcgg agattcagga
    13861 gatcgaaaaa aggctcggcg agctggaggc ggaggacgag ggagggactc ggaaatcttg
    13921 agatccggcc gcggccccgc ccgtcccgga caaccgaata ccgtgtttca tgaaaggatg
    13981 tgttccatga ccaaaggcgt gttcacgctt caagtaacac cgtttacgaa agacctcaaa
    14041 ctggatgaac cggggctgag gatactgatc caccgccaga tcgagagcgg cgttcacggg
    14101 atcgcccccc tcggcgtcac cggggaaaat cccgccttga ccgaggccga ggtcgaccgc
    14161 gtggtcgaga tcgcggtcga tgaagccggg ggcaaggcca aagtcgcgcc cgacacctgc
    14221 agcaacaacc tggagcaaac catcgaccgg gctcgaagat atgccaagat cggcgccgat
    14281 tatgtcgttg ttttcgcccc gtttctcgtc cttccgaccc aggccgggct gattgatttc
    14341 tatcttcgga cggccgacgc cgtcccggtc ccgatcatca tccacaacgc cccggcccgg
    14401 gtcggcgtca atctcgaaca caagggatat cagaggctga tggagcacgg caatatcgcc
    14461 ggtactaaag acggcaagaa ggagctggac cacttggcca agctcctcca catggcccgg
    14521 gacaaggaat tcgaggtttt cacgggcaag gatacgacgg cttacccgat gatggccttc
    14581 ggcggaagcg gaatgttttc cgtcgcggcc aacgtcgtcc ccggggtgat gcgcgggatc
    14641 gccgatcttt gcctggccgg gaagctggaa gaagcccggg cgatccatta tgcttattac
    14701 gacctcttcg ccgccctgcg ccttgaaacc aatccgatgg cggccaaggc ggcgttatcc
    14761 ctgatgggac ttcccggggg gccgctccgt ccgccgctca ccggtctcaa tgatcccaac
    14821 cgagaaaccg tcaggtccct gcttgcggag aaagggatga tttgatcgtg aaaatcgctt
    14881 tgaccacctc gggaaaaagc ctcgacgcgc tcgtggacag ccggttcggc cgcgcgccgg
    14941 gatttctcat ctatgacttg gagacgaaaa cgttttcggt catcgacaac gaacaaaacc
    15001 agaatgcttc gcaaggagcg gggatccagg cggccgaaac catcgtccgg tccggggtcc
    15061 gggccctggt gaccgggcat tgcggtccca aggcgtttcg ggttctttcc gcggccggca
    15121 tcgcggtcta cacggcggat gggctgaccg tggccgaaac gctgtcgcaa tatgtagacg
    15181 gaacgctcgc cgaggcatcg gcggccgacg tcgacgggca ctggtgaagg gcaaggatga
    15241 aaaaccggaa caacgtcaat gttttcatcg acgtgcttct tttcctcgtc ctggcggccg
    15301 tcgccggaat cggctggttg ctgaagttca aactacttcc gggacgggag cgcatcttga
    15361 aattcggaga aaaccgggag ctcctcttcc tgggatggga ccggcatcaa tgggggaccg
    15421 tccacttggc cgccgccctg gcgatgttgg ttcttctcgt tctccacatc gtctttcact
    15481 ggaaggcgat tcgatgcctg ctccgcaacg ccgttccctc gtctcccctg cgctggacgt
    15541 tgacggggat cgccgtcggc ctggccgctg tttttttcct gggggccttc gtcattgagc
    15601 cgacgaaagg ggaactggac atcggctttc accggaacat cgggccgaat gccggccgac
    15661 cggattacga tcggacgctc gccgcggaac cgccgtcgcc tgcggcaaaa gggaacatcg
    15721 aggccgcgtc cggggaagag ctcggcgaaa aactcgcgga agatcctctg ccggcggccg
    15781 ccgacgaaaa agatcatcgc cccggctcgg ggaatgcctc gttgaacggg cggatgacgc
    15841 tggatgaagc cgcggcggcc tgcgggattt ctcttcagga agcccggcgc cggctcggtt
    15901 tgcccgaaga ttatcccgga tcggaaactc tgggccggct gcggcgggcc tggggaacga
    15961 cgatgatccg gatccgagag cttttggaga atccgtcctg aaggaaagcg gcgttccgaa
    16021 tgaaaacgag ccggtcaggg gaaaaacgat cgccgtcatc ggctcacgat ttgatcggat
    16081 tccctcgatc cgccgggaag ggcgggaaca agcgaacggt cgaacaaccg cccgtcggcg
    16141 ggattgaagg acgcccggag agttgaaacg ttgatccgga ataagacaag gaaaggcggc
    16201 gcagcacatg agcgagacgc gaaaagaaac aaggccggga ggcgggatga agacgtcttt
    16261 ggactgcctt ccgtgttttg tccgccagac cctggacgcc gcccgcatgg tttcgacgga
    16321 tccggcgttt catgagagct ttctgcgcga gatcctggcc cggatcaccg agatgtccct
    16381 ggatacggcg ccggcggtct tcggccggga gcttcaccgg cggatccgcg ggctctccgg
    16441 ccgcggggat ccgtaccggg aagcgaaaga gcggcagaac cttttcgccc tggccttgat
    16501 gccgggattg cgggagcaaa tatcggccgc gccggacccg ctgatcctgg cggcgagatt
    16561 ggccgccgcg ggaaatgtca tcgatctcgg cgctcccggc gcgatctcgg aaaccagcgt
    16621 cagggaatcg atcgcccagg ctatgatccg gccgttggtc ggagaggaaa acggattccg
    16681 ggaggagatc ctctccgccg agaacattct ctatctggcc gacaacgccg gagaaatcgt
    16741 catggatcgc ctgtttattg aacggctggg gccctcgcgc gtgacggtcg cggtcagggg
    16801 cgggccgatc ctcaacgacg ccacccgggc cgacgcccgg gcggccgggc ttgctgaaat
    16861 cgtcgagatc atcgacaacg gctcggatgc gccggggacg ctgctggagg attgttcccc
    16921 ggaattcctg aggcggttcg aggcggcgga cctcgttttg gccaaaggcc aggggaattt
    16981 cgagacgctc gccgggcatc ccaaaaacat ttttttcctt ttcaaggtca aatgtccggt
    17041 catcgcggcc gaggcgggcg cgccggtcgg ggcgcatgtc ctaatccggt ccgccggggc
    17101 gcaagggagg ctcgcatgac ggcggatccc cctcgtcaaa gcgatatctc gtttcgcctg
    17161 agggctttgg aatacggatt ccgggatcgt cttcattctc ccgagaagat ccttcgaagg
    17221 gcgggagtgg ggccggggat tattccgtct cgccggccgc gcgccgcgga tgcttcaaga
    17281 cgaatgcttg gacgtctccg ggaagccgca tgatcctggc gattgcgtcc ggcaagggcg
    17341 gcacggggaa aacgaccgtg gccgtcaact tggcccgcgt gctccgctcc agggcctggc
    17401 ttttggattg cgacgtggag gagccgaatg ccgggctgtt tttgaaaacg agccggagga
    17461 ccgaggagat cgtttctctt cccgtccccg aggtggatga agcccgatgc gacggctgcg
    17521 gcgaatgcgg gaaattctgc gcttatcacg ctatcgtctc cttcgggacg atccccctcg
    17581 tctttccctc catgtgccat ggctgcggcg gatgcgtccg ggtttgtccg aggaaggcga
    17641 tccgcgaaac ctcccgccgc ctcggggtgg ttgtttcgtc gaaatcccgg gggatcacgc
    17701 tgatcgaggg ccggctggac gtcggggaat ccctttcgcc ccctttgatc cgggcggtca
    17761 aggccggcct tccggccggg cggccggcga tcctggacgc ccctccgggg acatcatgcc
    17821 ccgtcgtcgc cgccgtccgg ggcgcggatc acgttctgct cgtgacggaa ccgactccct
    17881 tcgggctgca cgatctccgg ctggccgtcg accttgtccg ggagctcggc cttccttaca
    17941 gcgtcgtcgt aaaccggatc gggaccggag acaaccgggt ccaggaatat tgcgcgggaa
    18001 agaagattcc cgtcgttctg gaaatcccgg acgaccggag gatcgctgag gcttattccc
    18061 gcggccggct gatcgttgaa acgctgccgg aataccgggc gctgttcgag cggctggccg
    18121 agaagctatt ttccgcggac ggggaaagat gcggattgga gccatgaacc atgttgacct
    18181 atgagtcctc gagcgaacgg agatctgccc gctttccacc ctcaaggatc attatgaaat
    18241 cattcgaggc tcggacaagc gcggacgagc cgaggaaggc gtgaccggca tggaagaact
    18301 cgtcgtcatc agcggcaagg gcgggaccgg caaaaccagc ctcacggcct cgttcgcggt
    18361 tctggccggc cgtccggtga tcgcggactg cgacgtcgac gcggccgact tgcacctggt
    18421 gctctcgccc cgcgtccggg aacggcacga gttccgaagc gggcgggagg cggtcatccg
    18481 gccggaggcg tgcaccggct gcggattgtg ttcgaccctt tgccggttcg gcgccgtttt
    18541 atccgcgtct tccgaaccgg atgtttcccc gggggccgcg gattgccgag actgcggcta
    18601 ttgcgtccgt tcttgtccgg tccggacgaa cgccgtgatc cgggaaatgc ggaatgcgtc
    18661 gggcggggaa gttccttccc tctatgccat cgatcccgtc gcctgcgagg gatgcggcgt
    18721 gtgcgtccgt ttttgccccg aacaagccgt tgattttccc gagacggtcg gcggggagtg
    18781 gatgatttcg gaaacgcgtt gcgggcccct cgttcacgcc cggctggccc cggccgccga
    18841 aaactcgggc aagctcgtct cgaccgtccg gcgcgaggcg cgccgcctgg ccggggagca
    18901 aggacgcggc ttgattctcg tggacggccc tcccggaatc ggctgccccg tcatcgcttc
    18961 ggtgacgggc gcctcgcgga ttttgatcgt caccgagccg accgtttcgg gagagcatga
    19021 cctggagcgg gttctttccc tggccgggca tttcggcatc ccggcctccg tttgtatcaa
    19081 caaatgggat ctcaacccgg aaacgtccga ccggatcgaa gcggcggcgc ggcggaaagg
    19141 agcccggctc gccgggcgga tccgttacga tccggccttc acccgggctc aagtgcggga
    19201 aaaggcggtc gtggaaatcg atgctcccgg cgccggggac atcaggaacg tttgggatcg
    19261 gttggaattg atgaagccgg gcgacaaaca tggatgaatc gaaatccgaa gtcgtttctt
    19321 cgatcgccct ggatcatgcc atgcatccgc ggcgcctcgg acgggcgaag gacgcggacg
    19381 gccgggcgcg gatcaccggc ccgcgcggcg agatcacccc gattctccgc ggcggcgcgg
    19441 gcaaacgaat cgccgacgac atgggtgtcc cgttctgcgg ttccatcccg atcgacccga
    19501 aaatcgccga agccggcgat gagggaagcg tttacgtacg gcatttcgcc ggatcgccgg
    19561 cggccgggat catgcgggag atcgttcgga cgatttttga ccggcggggg aggcggccgg
    19621 aagccggaga accggccgca tcaaatggaa ggaaaatctg aatccacaaa atgacaataa
    19681 taaaggagtc cctctgatga aaatcgcgat tcccatggcg aacgggcggt tgtccgctca
    19741 tttcggacac tgcgaaaaat tcgccctcgt cgaagtcgat ccgaaggaga aaaaaatcgt
    19801 cggccggacg gacatcgacg cccctccgca cgagccgggg cttttgccgg gctggctggc
    19861 cgaacaaggg gcggaggtga tcatcgccgg cggcatgggg gggagggcca tgtctctttt
    19921 cgccgagcgg gggattaaag tgatcgtcgg cgcgcccgtg gagacgccgg agcgtcttgc
    19981 cggcgatttt ctcgccggaa cgctgagccc gggcgataat ctctgtgatc attgagagcc
    20041 ctaaggatca gcctcccggc cgagggggga ccgcggcgat cggaggcttt cacttgcttc
    20101 gggcggaccg ggagcgcttg atttcgacgg cggccgcgct cagggagatc aatcccggtc
    20161 tcgtggtccc ctgccactgc acgggagaaa aggcgatcga gtttcttcgg accgagttgc
    20221 cggggcgcgt cgaggccggg agggcgggca tggcgctccg attctgaaaa aataattccg
    20281 tttttctctg aaaagatgat aatatacgtt tccgatcagt tttatttgag gaggatatcg
    20341 gatgtcgacc ctggagatcg atggaaaagc cgttgttctg aacgaggaag gattcctgga
    20401 gaaccccgaa gagtgggaca aacgcgtggc cctcgccctg gctaaacggg aggaaggcct
    20461 tgacgaattg ggaccggagc attgggcggt catcgaatat atccgcggat actacgtgga
    20521 aaaacagctc gccccgatgg tccggaagat ctgccagacg acgggcctca ccctcaagca
    20581 tatttacgag ctgtttccct cgggaccggc caagggggcc tgcaagctgg ccggcctgcc
    20641 caagcccgac ggctgcgtct gagccgcgac tctttttcgc cgccgccgcg atgccgccgg
    20701 cttaggaaat cacaagaccg ttgttctacg gtccccggcc gtctcggcga cggccggccc
    20761 ggacgagccg cgtccctccg ggcccgtttc ccgccttaat ccttggccgg cgcggccggc
    20821 ggccggaagg ccgcggccag aaagatcgcc gcgcccaggg cgatcaggca aggcagcagg
    20881 aacatcgact tgtagtcggt gacccccgcc gtggtgaaga ccgactgcag cagccaaggc
    20941 cccagggagt tggccgccag aacgcccagg ccgaggatca tcacgttgaa caaaccctgg
    21001 gcgctggagc ggacgtcctt gggaaaatac gtgtccacga agatatagac ggtggcgaag
    21061 aagaaagcgt agcagatgcc gtgcaggatc tggacggtta tgatcatcca aggttgggag
    21121 gggaagaagg catagaccgc gaaccgggcg gcatggccca ggatgccgac gagcatcgtg
    21181 atccgccatc ccagcttctt caacaccacg cccaggatga gcatggtcag gagctccgag
    21241 acctggccca ggctcatcac cggcatgatc cagttgccgg cgatgcccac gccgcccgcc
    21301 gccggcgccg ctccgagaaa ggttccggtc cagttgaaat aaaggttgtg gacgaaggcg
    21361 tcgatgaagg cgatgagcca gagaatcagg acgaagggcc gcccgagcag cttgaaggct
    21421 tccagccagg cgaagcgttc cgtcccgttc gcggcgcccg cggccttctt cttcggcggc
    21481 gtgtggggca ggagcaggct gaaggcggcc agggccaggg aggcgacggc ggccacgatg
    21541 aaggtccagc gggtggcctg tttcagcgcc tggccggtga gccccgagcc caggaccgtg
    21601 ccgatccagg cgaccatgcc cttgggcgcg gccgccttga cccgggccca atcgaccagg
    21661 atgaaggtga acggccaggc ggctaaaatc cagcccaagg tgccgcccat gcggatgagg
    21721 ccgaactcct tctggggatc cttcatgttg gcgaaggcga tcgagttggc gatcgagttg
    21781 gtcgggacat agaacaggca atggatccac atcaggatca ggaacggcca gaaggcccgg
    21841 gtgaagccca ggccgagcat ggccaggccg ccgatgaggt ggctgaagcc gaggaacttc
    21901 tcggccgcga aatggcggtc ggcgaactgg ttgctgaaga acatgccgat gatggcggcg
    21961 atggggaagg cgttcaggat ccaggcctgc tcgacgggcg tgaacccgag gctgggcagg
    22021 tagctgtaga tcagaggcag ccaggcgccc cagatgaaga actccaggac catcatgaag
    22081 aacagcttgt tgcgcgggct cttggtcacg gcggtcatgc gggatcctcc ttgcgtctca
    22141 cggttcccgg gggccgcccc ggtcgccgcc tccaaacatt ggatggagta taaaagagag
    22201 ccccgggggc gtcaaggacg agcgggcggg gcggcccgcg gctcttgcgg cgatatcggc
    22261 aaaaaggttt tactaaaaag cgatccccag gcccgctttg agataaaaac cggagagatc
    22321 cacggccgcg tcccggattt tggtgaggcg atgcccggcc caaaggccgg gcatgctcag
    22381 cgacgcgtag accggtcctt ccccggaatc gtcgaattca taataataga gcgggacgtc
    22441 cgagctctct tcccaatatc cgccctcggg ataaccccag aaataagtga aatcgccttt
    22501 gaaccccgtg atcttcgccc gacggtagcc ggcgtcggcc cacagccgga gcaccggggc
    22561 cggggcgatt tcgattccca ggccggcgga gaatcccggc ttgatcgcgg tggttttcag
    22621 cttccaatcc atccagagga cccggtcccc ggttttcagg ctgacgttcg tccaggatcc
    22681 cgcgtcctca aatgacgtcc ggtgcaattc gcccgcggca taaggacgga acgaaatcca
    22741 atccgcgatc gggatctcat aggcgagccc cagccgcccg taatcatcgc ggattttgtc
    22801 ctcgcggagg tttatcttgt cgacggccgg ataagtgacc gtgaactcgt ttcccgtcgt
    22861 ctcggctttg atcatcccgt aggcggccag aatctggacg cgcggctcga tcggaacgga
    22921 caacgccgct tcgaaattcg ccgccttgcg gacaggcttg aacccgagcc cccgggcggt
    22981 cgatctcgaa tcggcttccg cgatccggat ggaatcgcgg agaaacgaat tgagatcggc
    23041 gggggagatg aagcccggtc cgacggaaat ccggatctgc caggccccgc ccccaaagcc
    23101 gggaagcgca aacccgatga ggataaccga cacggggagg atcgggaggg gggtctcaat
    23161 atccatgaaa gcatcccaac gcgcgttgcg gccggtctgc ttcgcttgcc tccgtaatgc
    23221 gcctgagcgc gtcacgcctt cggaaaaata attcggttgg aagtcgccgg ccgatcgcgg
    23281 attggaggaa gagccgcaca aggggggcgc gcgccggccg tccagctcag gtccgaagga
    23341 ggcgtccggg cctcagcgtt ttttcgccgc cgcggcgatg tcggcgacgg ctgcgatcgc
    23401 ggcctcgatg tgggcttcgg tgttgaacgg cccgatcccg aaccgcaccg cgccgtggat
    23461 cttgtccgtc ccgaggcgct cgtgaacgag cggggcgcaa tgaagcccgg tccggcaggc
    23521 gatgttatga tcgacgtcga gcatcgttcc gacgtcggcc gcgtccattc cctcgatgtt
    23581 gaaagccagg acgctgatgt gatcgcggag gtcgtccaga cagtagagct tgacccgggg
    23641 gatattccgg aggccggcgg caagcttttc ggccaaggcc atttcctggt gatgaagagc
    23701 ctcgacgccc cgggcctcga cccatttcac cccggcgtga agaccggcga tcccgggcag
    23761 gttgggcgtt ccatactcga gccggtaggg atattcctcg aggtggagct tctgggcgga
    23821 ccggacgccc gttccgccgg cccgggtctg cttgatctcc acgccttccc ggacgtaaag
    23881 ccccccgatc cccatggggc cgagaagcga tttgtggccg gtgaagcaaa cgacgtcgat
    23941 gaagtcggct tccaggtcga tcgggatctt gcccgccgac tgggaagcgt cgatgacgaa
    24001 gaaaatgccg cgggcccgac atcggcgccc gatctcgcgg atcggctgaa tcgtgccgat
    24061 gacattggag gcgtggttga cggcgacgac ccgggtgttc ggcttgaacc gtttctcgat
    24121 ctcgtccgga tcgacgaagc cgcggtcgtc gaagggaacg atgtcgagtt cgaccttgcg
    24181 gtcccgagcc aggtggtaaa ggggccggag cacggagttg tgctcgacca tcgtcgtcac
    24241 ggcgtggtcg ccctcgccga gaagacccca gagggccagg ttcaaagcgt cggtggaatt
    24301 gtagccgaag cacagccggt tcgggtcggt cccatgaaaa aaccgggtca tctgtttgcg
    24361 cgtgttatcg acgacttcgc cggcctcgag gcagagatcg tagccggagc gtccgggatt
    24421 gacccccgct ttacggtaaa gcttgtccat gaacatgtag acttcatcgg gtttgggata
    24481 ggatgtcgct ccgttgtcga gaaatatgaa ttcggacatt tcgtttctcc ttaagtgaat
    24541 atcattttta gaaagagtaa tcctatgaaa agagtgcgaa aaaagcaacc cgatgtattc
    24601 tcccggaaaa ataatgggaa tgatggtata atcgccttcc cgccgccggg cgtatcatgg
    24661 gcgctttctt tcgtccgcgg ccgacggcgg aaatcagcga gggagaaaga cgcgtgagtc
    24721 ctttgttaca gtttgtcgag gacaggcttc aaatcgccgc cctcctgttc atggcttccg
    24781 tttatgtcat ccgggtgctt tggcttttcc atttccgggc cgtccgcgag cgggcggccg
    24841 cggaaggacg agcctcggcc ggagccgggt attccctgag caatatcgcc cggccttggg
    24901 cgatggaatc cacccggacc cggccgctgt tttatcttca atttgtcgtc tttcacttgg
    24961 gcgtcaccgc ggccatcgcc gcgacgttca tcattccgta cgcgccgcag tggcttgcgc
    25021 cgcggggccg ggtgatcgct tttcaagcct tgatggccgc ggcctttgtc gtcgccgttt
    25081 tccggctcgt ccggcgcttg cgccgaccgg tcctgcggct ggtgagcacg cccgatgatt
    25141 acgccgcgat caccttgatg atggcttatt tcatcctggg gatcctggcc gtacccaacc
    25201 gcccggaccg gggggagggg ccgatgatcg cctttttcat cctgacggcc tttttcctgg
    25261 tggtcgtccc cttttccaag atcagccatt acctttatta tcccttcacc cggatcattc
    25321 tcggccggac gctcggccgg cgcggcgtcc tgcccgtccg gcgcccgcgg tctcggcaaa
    25381 cggcggagga aagaggggag gaggtgggcg catgaaaacc gtaaaagcgg ccaaatccct
    25441 ttccgtcgag aacctccccc tggacgtggc tacccaggac aaggcccggg cggacgcctt
    25501 gccctcgatt caaaaagaca tgctgaggac atatgatccg gcggccgaca acgtccgccg
    25561 ttttctcgat ctcctcgccg aacggatcac ccgtccggtg gccgcctccc tggccgcctg
    25621 cgtccattgc ggcctgtgcg tggagtcctg ccattacgcc ctgactcatc cggacgatcc
    25681 gacgatgacc ccggtctaca aggcggatca aatccggaaa atcttcaagc ggcggatcga
    25741 ttggaccggc cggatcttcc cctggtgggt caaggccgga acgccgcgcg gcgacgagga
    25801 cctgaaccgg ctgaagaata tcgtcttcgg cacctgcagc gcctgccggc gatgcaccct
    25861 gaactgcccg atgggggtcg acacggccat cctgatccgc ttcacccgcg ggctcctgac
    25921 cgagctcggc atcgttccgg agggcgtctt caacgtcagc cgggacgagt gggagacggg
    25981 aaaccagatg gccgtcacgc ccctggactt cctggaaacc ctccagtgga tgcgggatga
    26041 cctgcgggcg gacccgaagc ttgccgggct ggacatcccg gtcgaccttc ccggctgcga
    26101 cttcatgtac acgatcaatc cccgggagat caagttcgat ccgcggtcga tcgcccaggc
    26161 ggccaagatc tttcacctgg ccggagagaa gtggaccttg ccgagcgccg gctgggatca
    26221 gacgaatttc ggcctttttt ccggcgacga caggctgggc ggggccctcg cccgcaatct
    26281 ctacgaggcc gcgatccggc ttcgggccgg gcgcatcgtc atctcggaat gcgggcacgg
    26341 ctaccggtcg acccgttggg agggatacaa ttgggcgcaa tatgatcagg acctcccgtc
    26401 cgagtccgtc gtccggacgc tgcagcgcta cctccgggag aagcggatcc gggtggacaa
    26461 gacgaaaaac gacctgccgg tgacgttcca tgattcctgc aacgtcgccc ggtcgggcgg
    26521 catgaccgag gagccgcggg acgtgctccg gcgggtgtgc gccgatttcc gcgaaatgac
    26581 gcccaaccgg acggagaatt actgctgcac cggcggggga gggctgcttt cgatggccga
    26641 gtaccgtcct ctccggatgg aggcggccgc gataaaggcc cgccagctcg aggcgacggg
    26701 ggccaagctg gtctgcacga tctgccacaa ttgcgtggac ggcttgaacg atgtgatcaa
    26761 gcattataag ctggacatgc gggtcgtcca ggtcctcgaa ctcgtcgaaa acgccctcgt
    26821 ttattaaaag gaggagttcc gtgacaaaaa tcgggatcat catttgcggc cgctataaaa
    26881 cgtgcggcgg aggaaaatgc ctccgggccc ttcgggaaag agtcggcgcg ttcgcccgct
    26941 atccgaaaaa cgaggccgtg gaaatcgtcg gctacagcta ttgcggcgac tgccccgggg
    27001 gaaacgtgga gtacgtcccg gaggaaatga agaaaaacgg agcggaggtc atccacctgg
    27061 ccaccgggct cgtcgtcgga tatcctccct gcccgaacat ccggaggttc aaggagctga
    27121 tcgagacgaa attcgagatt ccggtcgtca tcgggacgca tccgatcccc ttgaagtatg
    27181 cccaggctca tgaaaagctt tctttctgga aaagcgcggg gatggaagcg ttggccggcg
    27241 ggctgatgag ggaggacaag aagatccagg aagcctacaa ctgagcgggg gcggtttgcg
    27301 atattatctc ggcctcggcg gcaacaccgg cgaccggttc gcgaacctcg aggccgccgt
    27361 ccgccgcctc cgcgaggaag gcgtccggat catcggccgt tcctccattt accggaccga
    27421 gccggtcggc ctgcggaacc agccgtggtt tttaaaccag gcgctgaagg tcgaaacgcg
    27481 gctttctccg gagcagctgt tgaagacggc gaaagccgtc gaggccggaa tgggccggaa
    27541 accgggccgg aggaacggcc cccggccgat cgacatcgac cttctcctgg ccggaaagcg
    27601 aaacgtccgg accgccggct tgcagatccc gcatccgcgc ctggccgaac gccggttcgt
    27661 cctcgttccc ctggctgaga tcgccccccg cgccgtccat cccgttttga gaaaaaccat
    27721 ccggtcgatt ctccgcgatt gcccggacgc ctcggctgtc cggcgcttgc ggagggattg
    27781 aacgcccttt cccccggcgc tcccgtcgat ttgcaaatga cgggacgaat attcataatg
    27841 atagatcatg aaaagaatga tcgttctcat cgtgacgttc atattcatcg cgcccgtctt
    27901 cggggcggac gtggtcgccg tccacggcac ggtcgcgagc accggaggaa agccggtgtc
    27961 cggagcggtc gtcttgcagc gggaaagcgg cctgaaaacc acgacgggcg aagacggctc
    28021 gttctccctg gagctggaaa gatccggtcg gaccgtcctg gaagtcatcc atcccgatta
    28081 ttccgacgga ctgttcctcc ttccggcgaa atcgctcgat cggccggtcc ggctgactct
    28141 cgcccccctg attcagcaga acgaggaggt cgtcgtcacg gccctgcgct atcccgagcc
    28201 gacggcccgc atcccggccg cggccacggt gctgtcgtcc gtttccctgg gggacgcccc
    28261 ggcggccaac ttgaccggcg ccctggagcg ggcgatcggc gtcgcccccc tcggcaccgg
    28321 cgggttttcg atggttccca cgatccgggg catggcccgc aaccgcatcc tcatcctgat
    28381 cgacaacgcc cggatcgcct ccgaccggcg gacggggccg agcgcctcgt tcgtcagcgt
    28441 cgaggatatc gaccgggtcg aggtcctgcg cagcccctcg tccatcttct acggatcgga
    28501 cgcgatcggc ggcgtcatcc atatcttcac caagtcgggc cgggaggaag gaatccacgg
    28561 aacggtccac gccggctacg gcacgaacgg ggcccacgcc gagtacgggc tcgccctgag
    28621 cgggaaaagc ggtccttttt ccttctacct ttccggccag aacaacaagg ccgggacgta
    28681 tgactcgccg gacggcgagg tcggccagtc gcagtacggc caaaccggat ttttcggccg
    28741 ggcggcgtac gagaccgagg cccgccgggt cgacctgagc ttcctcctgg gacgcgggac
    28801 ggacatcggc aagccggcga aaaacacgcc caccaagccg acgtggtacc cccgggaaaa
    28861 ccagaacctc gcccaactgc actggatcga gaaaaacgtc ggcggcggcg agctgagcgt
    28921 catggcttac gccaacccca atttcctgga aacgcggacc gacacgatca ccggagccgg
    28981 cgggccgggc gcttatattt ccaagaaagc ctacgccaag acggagagca ccgaatacgg
    29041 cgcccagctg tcattcacca agccgctggg cggagatttc cgcctgaccg gcggagccga
    29101 tttcttcggc cggacgaacg ccggggcgat caacaacgag acctcttatg acgcgtcggg
    29161 gaacgtcaaa aaaatcctgg aggagacgcc gtatgccggc ggccgccggc gcgacctcgg
    29221 attctatctt tcggccgatt atgccgggat ccggaacgtc gacttgatcg ccgggctccg
    29281 cttcgacacg gtgaggcaga gcgcccggcc cggcggaaaa ccggacgagc tcgcctcgaa
    29341 cctgaatgcg gccaccggct ttttcggagc ctccatgcgg ctgacggacc ggtggacgat
    29401 cttcgccaac gtgtcccggg cctaccgcac gcccggcctc ggcgagctgt tttattcggg
    29461 aatcaccggc cgcggcgtca tcatcgccaa gccggacctc ctgccggaaa cgagcctcaa
    29521 cggggacgtc ggcgtccggt atgtcggcaa gaggtttttt gtcgggctgt acggctttct
    29581 ctacgggatc gacgacctga tcgaccgcct gctcgtcgcc cccggcgtct attcctatct
    29641 caatgtggaa gacgcccgga tctccgggtt cgagctcgag gccgaatggt gtccggccgc
    29701 cggctggagg atcttcggcc aatgcgccgc cctggacggc cggagccggg cgacggggga
    29761 tccggtcaac gacatcccgc cgttccgcgt tcaagtggga agccgattcc tgtggcgggg
    29821 cttgtcgctc gaggtttcgg gctcatacca agcgccgaag gatcgtccag ggggagctga
    29881 aatcgccatc ccggcctatc actacattca agcccgcctc ggctatgatt tccgttcggt
    29941 cggaattttc gccgtcgtga aaaacgccct caacgccgcc tacttcggcc ggccggatcc
    30001 cgacggcgtc tacgagccgg gccggaacat ccttttcgga gcccgctaca gcttctgagc
    30061 gggggatcga cgggaaagac aaggccgaaa cccgtttcat tgaggcggga tgaggattcc
    30121 cttacggttt tttcacggag aaagaggcct gaccctgttt cacggccgcg gcggcgggac
    30181 gcggagcgcg cccgggtcaa aaagcgtccc ggatatggcg gacgatcggt cgaccatcct
    30241 cctggaattc caccgccaga acatcgaagc ggcagggaag gccggccgtt tccgggtgtt
    30301 cgtagagata gctccgggcg attttccgga tttggcggcg cttggccggg gtcacggctt
    30361 cctcgggata gccgagccgg ggatcagtcc gggtcttgac ttcaacgaaa acgagagccg
    30421 gaccgtcacg ggcgatgatg tcgatttccc cccggccgta gcgatatcca cgctcgacga
    30481 tccgaaagcg cccgccggcg agatagagga cggccgcctc ctcgccgcgg cggcctagct
    30541 cgattctttc cttggagcga ttttccagcg gattccgtcc ttcgtgtctt ccagaacgat
    30601 cccggcctcg agaagctcgc ggcggaggcg gtcggcctcg gcccaattct tggccttgcg
    30661 ggccagctcg cgggcttcga ttttctcccg gacggccgcg gatacctcct cttcccgccg
    30721 ttcggggagg acggccagca cgtcgtccac ttgagagacg aaggaacgga gcgcccgggc
    30781 ctcatccgcc ccgatttttt cttcccggat cagggaattg gccttgcgga tcaggtcgta
    30841 gagggccgtg agggcggacg agacattgag gtcgtctccc aatccgtcgc ggaattcagc
    30901 gtccgcctcg gcgatcaggg gaccggccag gcccgagccg gcgtccggga aggcgcgatg
    30961 ctcgagctcg tagacgaaat cggtgatccg cttcagggcc gcggccgctt ggtccagcgc
    31021 ctcgaaggtg aaattcagga ctttccggta atgggtggag atgaggaaca tccggaggac
    31081 ggagggatcc acccgccggg tttcgacgag gtcatcgaga gagtaaacgt tgcccttgga
    31141 cttggacatc ttctccccgt cgacgatgag gtggtggcag tggagccagt accggacgaa
    31201 cggcttttcg aaataggcct cggattgggc gatttcattc tcgtgatggg ggaagatatt
    31261 gtccacgccg ccgcaatgga tgtcgaaggt cggcccgaga aaggccgcgc tcatggccga
    31321 gcattcgatg tgccagcccg gccggcccgg cccgatctcc gtctcccaga agggctcgcc
    31381 ctccttgggg gctttccaca aggcgaaatc gcgggcgctt tccttttcgt actcgtccgt
    31441 ttccagccgg gcgccgatcc gggacgtcgt ccggagggcc tcgacgtcga tccccgagag
    31501 ccgcccgtaa gaggggaact tggagatgtc gaaatagatc gatccctcct tccggtaagc
    31561 gacgcccttg tcgagcaggc ccttgatgat ccggaccatg tcgggaatgt gctccgtcgc
    31621 ccggggataa tgatcggccg ggtggatccg gagcgtctta aggccggcga aaaacgactc
    31681 gatga
//
LOCUS       DAOOXH010000019        30552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_1282_length_30552_cov_8.152048, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000019 DAOOXH010000000
VERSION     DAOOXH010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 30552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30552
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1282_length_30552_cov_8.152048"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="PKV64_03940"
     CDS             <1..966
                     /locus_tag="PKV64_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16267.1"
                     /translation="RLLLLGLRAWAGRYYAAGPGPSAGKTATVSAGEGDGEILIFGTS
                     RSARAFDQETLAARLGKKIIKNAAAGRYPRYFDLYYLRYRRENRRPAAVVYGMDYFMF
                     EKKTLPSELRILDPAFEAHALNPGDGVNDASPLLSRLSRLFRKKPEIDDFLGALLRLR
                     TEEDEVMPPAADQGKGRDENPDHSPPLSSDPRKRRPFEPRRYQARPGAEGKDLAKLLT
                     VLEKDGVPVVLVFIPDFIGTNETNFEQARFKEDVGALAGLHGNAAVLDFNRPDRFDLD
                     NPDLFRDGGWGKSNSHLNFRGKAVFNRLFAEAVRPFLRSKTPGAI"
     gene            complement(970..2097)
                     /locus_tag="PKV64_03945"
     CDS             complement(970..2097)
                     /locus_tag="PKV64_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011357600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HPN16268.1"
                     /translation="MIRVEHLAKCFGETVVLKDVNAEIKRGEVISIIGPSGTGKSTFL
                     RCLNLLERPSGGHIWVDGRDVLDPRNDPNALRRKMGMVFQNFNLFEHMTVLDNLTIGP
                     VKLLRKTRGEAETRARGLLALVGLAARENAFPDELSGGQQQRVAIARCLSMDPEIILF
                     DEPTSALDPTMVSEVLGVIRRLAADGMTMAIVTHEMRFARDVSRRVFYMDEGVIYEEG
                     PPAGIFDAPKREKTRAFIFRIRNFQYHIADEKYDLYDLKAGIEAFCGRHFFPAAAAAD
                     LLLVVEETLQLCFREGDAAARAGLVSEAGGLDLTVGYSEQENETTLLFTAGEVLKTIL
                     NETAESDGLSRALIAGMTRSLREDIRGGKVVLTAVMREPGS"
     gene            complement(2133..5072)
                     /locus_tag="PKV64_03950"
     CDS             complement(2133..5072)
                     /locus_tag="PKV64_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPN16269.1"
                     /translation="MKRFAWIAAGGLLAGACLSCSPAGKPAGVASLEDLKTARFGVVS
                     GTVADQIVLNRFPEATFGYYPSPLDACLAVLKGKETAAAYDEPILRNIQAQNPGLKIL
                     PEMISVDQYAMAVRPDDRELKDAADALLAELRADGRYDEMKARWFPEKGKPAPMPEMA
                     WEAPNGILDFGTCAVTEPFSFIDDSRRVVGFDVELASRLARRLGRELRIHNMEFGAMI
                     PSLLSGKTDLIAACITVTEERAKSVLFTEPVYRGGIAAAVRDSAVAGAAPAFSGLADV
                     AEKRIAVMVGSTHDKHITANFPRAEILRMDNAPDMLAALKSGKCEAAIFDANAGRLYR
                     EDDPSLAVLAEKIFIENLGFGFADVRLRDAFNAFLGELRATGELDEIRRKWEGDDGAA
                     AIPDLPEGGENGRLRIATTTVDIPFSYTREEKPCGIDLELALRFAARQGLKPQIQPLP
                     FASLIAAVASGKADLICDGITITEERAKQVAFSDAYYQSAADALTLKEHLDGGNRPEE
                     TGSKPDIAEAVIGAMTGSTGEALVRERFPRAGIHLFDDVMDAVAALKARKVDYVITAY
                     TTALQAARANPDLAVLPEEYNQAWAAIAVHKDNPRLLERINAVLEEMRDDGTLEEIIS
                     HWVRPDGSPYVKTEVPRASGWPALRVAVAANREPMGFIEDNKIVGLDPELIERIAFKL
                     GRPVVFTDMKFSALIAALESGKADVVCSNLTATEERRRRVAFSEGYYVNPQVMMTLKD
                     RPPAAGAALRPSWPARLKKSFVDNIIREKRYLLIWNGLKVTALIAVLSALLGTLLGAL
                     ICLMRMGRNRALKAFAKAYISLLRGIPQVVLLMLMFYVVFAPLDVSGVAVAIFTFALN
                     FAAYVSEMFRAGIESVKRGQVEAGVAMGFSRFRTFVHIVLPQAVQRILPVFKGEFVAL
                     VKMTAIVGYIAVQDLTKASDIIRSRTFDAFFPLVMVAVLYFILAWGLTSLLEMIRVRV
                     NPKRRRP"
     gene            5205..5474
                     /locus_tag="PKV64_03955"
     CDS             5205..5474
                     /locus_tag="PKV64_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16270.1"
                     /translation="MKKKRVLKGILAPALLLAAAIGLGQPTPSAENPPQETDPARRPP
                     TDYMRSLLSQNTKPLGVKVDKSLERLMRQPAIAWTARAARRHILD"
     gene            5544..8402
                     /locus_tag="PKV64_03960"
     CDS             5544..8402
                     /locus_tag="PKV64_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN16271.1"
                     /translation="MKDRKIWLLVPLTILVVLGVLNVYRQIVYKEPSDGVVWAMKQGR
                     LTAIKVDKDGPAYLFNLKKGDVLFSVTHNLAPGKIIVRSKIDLIKNLWQVWKQGQKIT
                     YEIYREGGVITYTGTFFPVGKGPDIIYFYLALIGVITIIIALVVFLNTSRPFRRTNIH
                     YYFLSVLLFMFYVFSFTGEFDWLDSLFYGLDKIAFLVFPPLLVHYFLIFPQRVKLLRD
                     KPGTAFLLYAPASLLLLARIALFLPAAGGLGELRLLQAYSALEKLDLGHFALYTLGAL
                     ATIAHGTLRHPSFIIRKQLKWVAYGLGFGVVPFTALYVVPFVAGAAPSVAAELTILLQ
                     ALIPLSFSYSISRYKLMDLEVILKKASTLVFSYFVLALVFVGISSQTKIFSENKINAL
                     LLGALAIILGATMFTPLRTLVQSLFDRVFYRRSYQYRKTLLSISQELSRERNIETLVR
                     RLLELISNALSLRSIALLLPVETGSATFTIFSAHGNAETAAGGRLTFDPKIYETLVEN
                     NTLSLYSVTEDIKLQTKLESLRDSGFDHFLALKGESKLVGCLAMGRKIDTSFLTREDW
                     ELLTTISSPVALAVENAYLYNQAGLRASELERLKDYSENIIESLTVGVAVLDQNGRIS
                     GWNRVCEETFNRKREEVLGERLSDIIGPRAFAAIFPPDTQSEYHLVGEIPIGRPSGEM
                     RIFDIAKTPLRDNRRHPFGTIIVFEDITEKVRLQQQLLTSEKLASLGLLSAGVAHEIN
                     TPLTGISSYVQMLQKTVGDERFSQVLGKIEAQTDRVSRIVKNLLTFARNPAEPGMHRI
                     DLRDTLQEIISLIDYKLRAMEIELETDLAPVAPIWAQGERLQQVFINIILNALDAMPG
                     GGRLRVELRETPVEAVVVISDSGTGIKEQHLARIFDPFFTTKGVGKGTGLGLSISYAI
                     INEHEGRIEVQSEVGQGSKFTIFLPKDLDRRKSKTRES"
     gene            8399..9778
                     /locus_tag="PKV64_03965"
     CDS             8399..9778
                     /locus_tag="PKV64_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPN16272.1"
                     /translation="MSHASIHVIDDEPVIRDVLEQLLQAEGYDVTLSESGEAALSKFA
                     DHGFDLVLLDLLMPGRGGLEILPEIKKIQPSTIVIIITAYASVESAIQAMKIGAYDYV
                     QKPFKHGDLLLTIQRALAHKTLLDENDRLREELRKKFSFSNILGRSRPMERVFELIKA
                     AAPTRSTILVQGESGTGKELAARAIHTNSPRASFPFIVVNSGALPPDLLESHLFGHVK
                     GAFTGAVSEKQGLFEAADKGTIFFDEISTIGLETQAKLLRVMQDREFMRLGGTKTIRV
                     DVRIIAATNVELEDLIDRQLFRKDLYYRLNVIKIILPPLRERREDIPLLVKHFLDVFA
                     RENGKTIEGVSPDVLEILDRHSWPGNVRELENLVERTVVFSRDKVITRDCLPDLFRTP
                     REAAAAPAAEGDGLDLKEQTLAFQRRLIETALAKANGVQKTAAALLGVKPTTLNEMIK
                     RLEISPDRS"
     gene            complement(9860..12526)
                     /locus_tag="PKV64_03970"
     CDS             complement(9860..12526)
                     /locus_tag="PKV64_03970"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HPN16273.1"
                     /translation="MRRTRNIIGSVFCYNLTMRMQGWAAAFPLVLVFTLASFGQESLP
                     PQTDVRTFLSGLKEAFESSDRAAYLAYFEAEIRDAEGAAYDSLRQASRMTQAVFFPAT
                     NVFEDKEGVPLFVQAVFQNDLEALFESWELELARDGERRVIRAKSVIGNPAIFLKLKI
                     PSDRIERAARIEIRHDDLEATFENAWVFYDNIPDRPTGLIIIGSGRLRFRPSSETERH
                     QLALRYKTENLDKPLVSAYLRFSPSFFANRIKITPETGPAGSCPPGEIAKARALFRRY
                     YGGSFTIDNPIAGARLSVLPQSDQAVFEFGTDSRSGFGYGYSPFSEEEIHFWSRSPDQ
                     LLCLYSPDIREGNERRMFLTMGEKVDILRCDIDLDFQPDRRFLSALARIRLEAASPGV
                     DNLRFDFHSALDVLKVYDASRRELFFTQDKVNRLLYIRFLQPLEKGESASLDIYYRGA
                     LPPPPPTSDAAAAYGGFAGANFIDDGDLYTPSAGWYPAMSEGDYFLSRFRISLPPRYV
                     CVANGELAGVEEVDEVRRVLSLEKIGNRISVFETRKPVKGLAFIVGRFREDAVDEDPS
                     PPPINVLVSENVRSLRGTLAGEARAIVRFYEETFGPFPYEKLAIIQRVAETAGGQSPA
                     SFVILNEPRTGATAPGIPDFGSPVDLPGYRELFLAHEIAHQWWGHAVSAATYHDLWLS
                     EGLSQYAAIRYLRMKHGNDKLPSILKKSVSWTKRMADYGPITLGARLSYLDFRAFQAI
                     IYGKSTVVLFLLADLIGEEALDRGLRAFQASSAYRPVRTGEFIRALEEASGRSLRDFF
                     RGWIDSHVLPEASVTHQILKADEGYEVKIFVRQAGQAMVFPLTVAWTEDGRPVRHILE
                     VDSESKFYSFPAAVKPVKFKINPDGLVPGKFR"
     gene            12824..13852
                     /locus_tag="PKV64_03975"
     CDS             12824..13852
                     /locus_tag="PKV64_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005372093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HPN16274.1"
                     /translation="MKAMLITRILDAWESGEPLESRDLPLPVPGEGEILLRIAACGVC
                     HTEIDEIEGRTPPPRLPVIPGHQVIGRVAARGPGARRFKLGDRLGVSWIFSACGKCRF
                     CLEGRENLCPEFRATGRDADGGYAEYMVVGESFAFSIPSLFTDAEAAPLLCAGAVGYR
                     SLKLAGLRDGQALGLTGFGASGHLVLQTARHLFPRTRFYVFARSPEERVFAAELGAAW
                     TGEASDTPPEDLDAMIDTTPVWKSVVEALRCLAPGGRLVINAVRKEDGDKDALRALDY
                     AGHLWREKEIKSVANVTRADAVEFLRLAAEIPLRPAVQEYPLAEAGQALGEIKRRRIR
                     GAKVLRIG"
     gene            complement(13868..14920)
                     /locus_tag="PKV64_03980"
     CDS             complement(13868..14920)
                     /locus_tag="PKV64_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HPN16275.1"
                     /translation="MRTFGRSALGAFLAAAGLLAAGCGGGAKAPGILHVYTWSDYVKP
                     ELIARFEAENNCKVVIDTFDSNEAMYAKLKAGATGYDVITPTSYMVSLMQSQGMLLPL
                     DHSRLPNLSRVDPDYLAIAIDKTMAYSVPYMLTNTGIAYLKSKVKDFEPTWAMFNRSD
                     LRGRMVMLNDMRETIGAALKFLGYSLNSTNPEELSAAKNVVLAWKENLAKFENEQYKT
                     GIASGEFLLVHGYSGDILQVQAENEDIAFAMPREGGAISCDDLVIAKTAQEPDLAHAY
                     INFFHDPAVAAENTEFIQYLCPNKDSYPLLKAEITGNPGIFLTPEIKAHCEILADLGE
                     KNALYVKIWDEIKAGK"
     gene            complement(14923..15711)
                     /locus_tag="PKV64_03985"
     CDS             complement(14923..15711)
                     /locus_tag="PKV64_03985"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN16276.1"
                     /translation="MKRSRFPFIVTGLVLVFFYLPILILVVDSFNASRFGGAWRGFSL
                     KWYVRLFHEPAIWTAARNTLVIALIVTFVSLLLGTTSALALHRFSKSRFQRVHYTLIY
                     TPLVVPEILMGISLLMAFVAAGVKLGMGTIILAHVTFCISYVALVVLGRLQDFDFTVV
                     EAARDLGATGWTATRRVLLPMLAPGLAAAGLIAFTLSVDDFVITFFVAGPGSTTLPIR
                     VYSMIKHGATPLINALSTLLLALTFTTVLVSQRLSKSGALEGGQ"
     gene            complement(15708..16586)
                     /locus_tag="PKV64_03990"
     CDS             complement(15708..16586)
                     /locus_tag="PKV64_03990"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013181998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN16277.1"
                     /translation="MKNLRRRGQEFFLTLPSFFWLSLFFLIPTALVFILAFRPADPFG
                     GIGRGWTTATIKGLADPQYLAIAWRTLWVSFLSTAFCLLLAVPTAYAVARAPERRRNL
                     LLMLVIVPFWTSFLIRVFAWKSILHPEGLIKKTLVFCGLVDPSSQLLYNLGAVILVTV
                     YTFLPFAILPIYAAAEKFDYRLLEAARDLGARPFQAFRKVFLPGIRQALVTAVLVVFI
                     PSLGSYIVPDIVGGPGSEMLGNKIAQRVFVDRNLPLAGGLSIVLILAVLAPLLGVLAL
                     QGKKARIQPPEGGGKP"
     gene            complement(16621..17772)
                     /locus_tag="PKV64_03995"
     CDS             complement(16621..17772)
                     /locus_tag="PKV64_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN16278.1"
                     /translation="MAEFLEFKNVSKRFGAIQAVDGVSLEIKRGEFFALLGPSGCGKT
                     TMLRIAAGFETPDAGQVFLAGREITDLPPNRRPVNTIFQSYALFPHLTVRDNIAFGLQ
                     IAKKPKAEIDREVETMLRLIRLEDQAGKKPDLLSGGQRQRVAVARALINKPGVLLLDE
                     PLAALDLKLRQRMLIELDMIHDQVGITFLYVTHDQSEAMSLSDRIAVMNAGRIEQIGA
                     PAEIYEAPRTSFVAAFIGDTNFLEGVVADHPEPEYSRLRIEGLPDVTVYNDKQIADGH
                     KIFLSIRPEKIQISRDRPEPKPKRNAVPAVVEDVIYLGPTTKYWVRAGEHRLQVLRQH
                     ARFFLDEKPIRWKDDVWVFWHGDDGFMLEGFRRDDEKLVAAPPQKVGEA"
     gene            17855..18556
                     /locus_tag="PKV64_04000"
     CDS             17855..18556
                     /locus_tag="PKV64_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPN16279.1"
                     /translation="MNVISIVIQIFVLLFAITVHEAAHGWTAKKMGDPTAYNMGRVTL
                     NPIAHIDPFGTILLPLLLSLARLPAFGWAKPVMVNPRNFRNPRRDNLWVSFAGPAANI
                     SVALVSLILLFLMKLTIPGVGRFIQALINGFFRQMGFLLPVLSRGFYPLEGLALILIM
                     TVLINSYFALFNLIPVPPLDGSGVLMGLLSDQAAFRYDRIRPFGFIIVLFLIYMGFLD
                     ILMWPVKLMIVIVLG"
     gene            18553..19551
                     /gene="trpS"
                     /locus_tag="PKV64_04005"
     CDS             18553..19551
                     /gene="trpS"
                     /locus_tag="PKV64_04005"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPN16280.1"
                     /translation="MNPTPDPRKIVLSGVRPTGPLHLGNYVGALRNWIAFQDLYRCFY
                     AIVPWHALSSEYANAKAIRGWSYEVAVDWLSVGLDPEKCVMFLQSEVKEHAELHLLLS
                     MITPLPWLERVPTFKEQLKELQEKELNTYGFLGYPVLQAADIILYKADLVPVGEDQAA
                     HVELTREIVRRFNRLYGPIFPEPRTMLTEVPRLAGTDGRKMSKSYGNAIYLKDSAETI
                     RAKILPMVTDTRRVRRTDPGEPDDCPVFTLHKAFVPKEAREDIARGCRTAGIGCRDCK
                     AVLLEHLFRFLEPVWERRRALEKNPERIWEIFEAGNSRARRVAQATMAEVRDALGF"
     gene            19569..20375
                     /locus_tag="PKV64_04010"
     CDS             19569..20375
                     /locus_tag="PKV64_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPN16281.1"
                     /translation="MVDPGYPNEEGPLEKPAPEEESAVEGGGDGYQIKLDIFEGPLDL
                     LLFLIKKKKIGIEDIPLAVITREYLDYLHGRERINLDREAEFLLMAALLIYIKSQILL
                     PREQPVEPDIDPRKERPDRTEDYERVKVVSAVLRDKEEEQSTIWRRTALNVPLPEEDL
                     DLIEVSLFDLAESFFAMMKRKQAVPQRILKTKEISVADKIREIVSALREHGFLDFLEY
                     LDAQETLEEALVAFFCLLELIKARRVVAVQEQLFHTIKVWLRKEPAGEDA"
     gene            20375..20911
                     /gene="scpB"
                     /locus_tag="PKV64_04015"
     CDS             20375..20911
                     /gene="scpB"
                     /locus_tag="PKV64_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPN16282.1"
                     /translation="MDDLKNILEALIFISVEPLTMDKIKEVLDGTPEEEILQALLRLG
                     TEMEDAPRPIRLVQTGGGWVFMTKPAYDLHVRKLLQIERRKRLSSAALEALSAVAYHQ
                     PVTQTDIMNIRGVDSTHALHTLLENKLIKISGRKDAPGRPLLYRTTEKFLTYFGLNSL
                     EELPRETELKKILEEGNA"
     gene            21369..23117
                     /locus_tag="PKV64_04020"
     CDS             21369..23117
                     /locus_tag="PKV64_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPN16283.1"
                     /translation="MTEDAKQNNEKISAEDYENLLDKYQFSSQEVSPGKIVAGRVVKV
                     LPAHILVDIGLKSEGLIPIEDFTESADARIPSVGDEIEAVLERTDSKEGYFILSKRQA
                     DARRALELLEKAYEEGASISGRVIDRIKNGYTVHVGLKAFMPDSHAELRPLKDPAKIV
                     GQTLKVKVIKYDRKTENAVVSHRAYLMEERDKKKKIVFSQIQKGQKLKGLVRSLTSFG
                     AFVDIGGVEGLLHVSDMTWGKIANPHDLFKIGQEVEVLVLDFNEKDEKISLGTKQLTP
                     NPWDNITDKYKPGDRIEGKVSSLTDFGAFVELEPGIEGLVHISDLTWSRKMIHPKKLL
                     NLGDKVTVSILDVNPTSRRVSLGLKQTQPHPLEELKKRLVPGTRIKGRITSITDFGAF
                     LEVEPGIEGLIHITDISWEKIKHPSAKLKVGDEAEVVLLNIDVEKQKVSLGIKQLEGD
                     IWEEFFLRQKVGDLLNVKIVRIAEFGVFAEILPGIEGVVFNSEIDEKKVENPAEVVSV
                     GDQKTAKIIKLNPRDKKISLSFRQAQSDLQRQEYQKYQETQDDRLTFGDLIKDQLRQL
                     QNAKKSGAETEEKPND"
     gene            23110..23385
                     /locus_tag="PKV64_04025"
     CDS             23110..23385
                     /locus_tag="PKV64_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPN16284.1"
                     /translation="MIKADLINKISKELNIPKQEAETGVNMFFETIKEALLAGEEIEV
                     RGFGSFRFRNRPPRSGRNPRTGDPVKVPPKKVLYFKPSKLLKELINK"
     gene            23387..23899
                     /locus_tag="PKV64_04030"
     CDS             23387..23899
                     /locus_tag="PKV64_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPN16285.1"
                     /translation="MEYIAAPWREPYIRSTSKKETGCVFCRAARTRDDASAFVLDRGR
                     RAFVLMNKFPYLPGHLMIAPYRHSASLERASREVTGEMDELLRSCLRVLKKHYRPQGF
                     NIGLNLGRAAGAGVIDHFHMHVVPRWTGDSNFMPICGRTKIVLEDLETTYARLAPLFR
                     RERRNTPSKS"
     gene            23924..25858
                     /locus_tag="PKV64_04035"
     CDS             23924..25858
                     /locus_tag="PKV64_04035"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPN16286.1"
                     /translation="MLKSMRENVKSLKWVLWLVVAAFVVSIFFIWGASGKLTGDDAGN
                     TLAVIGRKKISGDDYLQALRNRIEAIKSQYQEIDRAFIEQLNLPQQVLEQLVEQTLLV
                     EQARAMRLRASDEELRDRITSIPGLQQNGKFVGYEMYKRVLQYNHINLSEFEHSLRQD
                     IVLDKMIRLLTAGLAVTPDEVWSDYQKASDSAGIDLLILETSKVSLDEAPSAAEVQAF
                     FEARKGAYLIPEKREGLAVFLKAEDLKKEIELSEKDIENYYQANLSRFTNPESVRVGR
                     IWLPFEGKDKALVEAEGRRVLERLGAGGDFAALARELSKDAKASGGGDYGFYDWQSLP
                     QPERDEIHRLTSGQTSDLIAEADGLAVIRVTEKTAATTTPLAGASAQIRSILLDEKAR
                     QLADTRIAKIEKEAKSARSLETALRKAGLKGESTGLLKNGEAWSDNDPSGAVGTALFG
                     LKEKEVSAPVYTYTGVGLVELRKVEAPRPAAFEEVKADVETALVNERKMETSRARLAA
                     LGRRLEEKNWEDIAARNGFEFKTVEAHKRDQYIAIVGESKTADDLAFTLPLNEASAPF
                     DFETGTAVLRVRDRKTVTREEFENVKETQTAQILNQKKNMFLQAYLAKLKQEKGIQIK
                     YDKFLQLTQDVLSRYESAKQ"
     gene            26172..26552
                     /locus_tag="PKV64_04040"
     CDS             26172..26552
                     /locus_tag="PKV64_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16287.1"
                     /translation="MKTKTMTAFLILAATAMTTAACFRSYGHRRYPGTPRFASNDYAY
                     VQLLRREPRRDHVRLGKVWFRPEPWMDRYEVKLILREKTARMGGDALIIVADRYPRGA
                     AARRYRSRAVGERWIIGFVIRYRR"
     gene            26855..27808
                     /locus_tag="PKV64_04045"
     CDS             26855..27808
                     /locus_tag="PKV64_04045"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPN16288.1"
                     /translation="MNPVKNKLYAAAEKAVTQSLRIKAGEQFLLVTDKAKMDIAEAFA
                     FWAKKCGAETTTYLMTETLRPISEPTKLFRMLAESPKVMVYMLDARIAEKPFRGYMVQ
                     AGETHGRLCMMPGITVDMMERLVNIDYFKLAEKTKRIMRLLKDAGDVRITNPAGTDIS
                     FSVKKRGWHIDIGDISKRGRHGNLPAGECYTCPVEETFTGKAVISLIDDKLGRGVMHF
                     ERGKLVSSSGRGIEAVLKNIGEDPTGRIIGEFGVGTNTGARICPNMLEAEKAFGTVHF
                     AVGDSYGIGVNKSRHHYDALIEKASIYAKGRWIARDGRYLV"
     gene            27884..27988
                     /locus_tag="PKV64_04050"
                     /pseudo
     CDS             27884..27988
                     /locus_tag="PKV64_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012799024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            28092..28682
                     /gene="fapR"
                     /locus_tag="PKV64_04055"
     CDS             28092..28682
                     /gene="fapR"
                     /locus_tag="PKV64_04055"
                     /inference="COORDINATES: protein motif:HMM:NF003359.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HPN16289.1"
                     /translation="MIRKEERHERLREEIERNPFVTDEELAAALGASVPTIRLDRSTL
                     SIPDVRRRTREMAGRYFGVSRSLGARDHVGEMIEIDPGKSGLSLLETESWMCLEKCDI
                     VRGHVLFALLNSLAAAVAEAPIALTGEARVDFLRPVRAGARLIAKARVEKKIGRRVFI
                     EAVARSGENIVARGRFVVHGLNRETAAHLHLLKDGE"
     gene            28683..29657
                     /locus_tag="PKV64_04060"
     CDS             28683..29657
                     /locus_tag="PKV64_04060"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HPN16290.1"
                     /translation="MPKGTIMTEIGIRSIGTSVPKKILTNADLEKMVETTDEWIVSRT
                     GIKERRVLGPDEKLRPHVVAAAKTALERAGLQGKDAGFIISSTVISDRFCPAQSYEIA
                     HDLGTERALAFDLNAACSGMIYGLGIAESLLQTRDLAWGLVTAAEQMTTATDYTDRAG
                     CILFGDGAAAVVVTKERPMHRLLATELGSIPSLVDEVQIGGAKDLLAGRTKNFWFRQN
                     GSTVMKFAVRIMQDLCERVPRKAGVDIKDVRYVIPHQANQRILDAAAKGVPGPAEFIS
                     VIGKYGNTSSASVGLALHDAWDRFQTGDLVLLIAFGGGLSWAGALIEW"
     gene            complement(29764..>30552)
                     /locus_tag="PKV64_04065"
     CDS             complement(29764..>30552)
                     /locus_tag="PKV64_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16291.1"
                     /translation="GGSAMPSPEEELRLLLPVIDRLTKAALFPKRLACRGAARIPETG
                     PVLLVSNHCGAIKDPAAVFRIVSRPLFFNANRMLFDRGELDFLIRKHLRRHLKNYGLV
                     LDRLLGPLKTVFVRFVSTNIARVGTIPADMYEGSNLTAVSLFMDYLRAGRAVVSMQGR
                     GRVHPDDPNPYVRRFGRGIPYIAFRMKAGEGLDIPVVPLSIFGTQRAWGIPGTIRVNV
                     GLPLYIRDFLGGDINETVERFRAALQEAVERLLVESLRNPFSSG"
BASE COUNT         6122 a         9712 c         9250 g         5468 t
ORIGIN      
        1 cgccttctcc tgctcggcct ccgcgcctgg gccggacggt attacgccgc cgggccggga
       61 ccttcggccg ggaagacggc gacggtctcc gccggcgaag gcgacggaga gatcctgatt
      121 ttcggaacct cccgctcggc ccgggccttc gaccaggaga cgctcgccgc ccgtctcggg
      181 aaaaaaatca tcaagaacgc cgcggccggg cggtatcccc gatatttcga tttgtattat
      241 ctccgctacc gccgggagaa ccgcaggccc gcggccgtcg tctacgggat ggattacttc
      301 atgttcgaga agaagaccct gccgtcggag cttcgaatat tggatcccgc cttcgaggct
      361 cacgccctga atcccgggga cggggtcaat gacgcctctc ccctcctgag ccgcctttcc
      421 cggctcttcc ggaaaaaacc ggagatcgat gatttcctcg gcgctctgct ccgtctgaga
      481 acggaggagg acgaggtgat gccgccggcc gcggatcaag gcaaaggacg ggacgaaaat
      541 ccggatcatt cccctccgct ttcctcagac cccaggaagc gccggccgtt cgagccccgc
      601 cgttaccaag cccggccggg agccgagggc aaggacctgg caaaactcct gaccgtcctt
      661 gaaaaggacg gcgttccagt cgttctcgtt ttcatcccgg atttcatcgg gacgaacgaa
      721 acgaacttcg aacaagcccg gttcaaggag gacgtcgggg ccctggccgg ccttcacggc
      781 aacgccgccg tcctggattt caaccggccc gaccggttcg atctggacaa cccggacctc
      841 ttccgggacg gaggctgggg caagagcaac tcccatttga actttcgggg aaaggcggtc
      901 ttcaaccgtc tgttcgccga ggcggttcgt ccttttctcc gctcaaaaac gccgggcgcg
      961 atctgacgtc tatgatccgg gctcccgcat cacggccgtc agaacgacct tcccgcctct
     1021 gatatcctcg cggagggatc gggtcatgcc ggcgatgagg gcccggctca atccgtccga
     1081 ttcggccgtc tcgttgagaa tggtcttcag gacctcgccg gccgtgaaga ggagggtggt
     1141 ttcattttct tgctcggagt agcccacggt cagatccagg ccccccgctt cggaaacgag
     1201 gccggcccgg gcggccgcgt cgccctcccg gaagcaaagc tgaagcgtct cctcgacaac
     1261 caaaaggagg tcggctgcgg ccgcggccgg aaaaaagtgc cggccgcaga acgcctcgat
     1321 cccggctttg aggtcataga gatcgtactt ctcgtcggcg atgtgatatt ggaaattgcg
     1381 gatgcggaag atgaaggccc gggttttctc ccgcttcggg gcgtcgaaga ttccggccgg
     1441 cggcccttcc tcgtaaatga cgccctcgtc catgtagaag acgcggcggg agacgtctcg
     1501 ggcgaaccgc atctcatggg tgacgatggc catggtcatg ccgtcggcgg ccaggcggcg
     1561 gatgacgccc agcacctcgc tcaccatcgt cgggtcgaga gccgaggtcg gctcgtcgaa
     1621 gaggatgatc tcgggatcca tcgacaggca acgggcgatg gccacgcgct gctgctggcc
     1681 gccggacagc tcgtcgggga aagcattttc ccgggcggcc agcccgacca gggcgagcag
     1741 gccgcgggcc cgcgtttccg cctccccgcg cgttttccgc aagagcttca ccgggccgat
     1801 cgtcagattg tccagcacgg tcatgtgttc gaagaggttg aaattctgga agaccattcc
     1861 cattttccgc cgcaaggcgt tgggatcgtt cctcggatcg aggacgtccc gtccgtccac
     1921 ccagatatgg ccgccggaag gacgctccag caggttcagg cagcggagaa acgtgctctt
     1981 gcccgtgccc gacggcccga tgatcgagat gacctcgccc cgcttgatct cagcgttgac
     2041 atccttcagg acgacggtct ctccgaagca cttggccagg tgctcgaccc ggatcaggac
     2101 gtcgcccgag ccggcggacg gacaggaagc tttcacggcc ttctcctttt cgggttgacc
     2161 cggacccgaa tcatttccag aagcgaggtc aggccccaag ccaggatgaa gtagaggacg
     2221 gccaccatga ccaaggggaa gaaggcgtcg aacgtccggc tccggatgat gtcgctggcc
     2281 ttggtcaggt cctggacggc gatgtagccc acgatggcgg tcatcttcac cagggcgacg
     2341 aactcgccct tgaacaccgg cagaatgcgc tggaccgcct ggggcaggac gatatggacg
     2401 aacgtccgga accggctgaa gcccatggcc acgccggctt ccacctgccc ccgtttcacg
     2461 ctttcgattc cggcccggaa catttccgag acataggcgg cgaagttcag ggcgaaggtg
     2521 aagatcgcca cggccacccc gctcacgtcc agcggggcga aaacgacata gaacatcagc
     2581 atcagcagga ccacttgggg gatgccgcgc aggaggctga tgtaggcctt ggcgaacgct
     2641 ttcaaagccc gattccttcc catccgcatc aggcagatga gggctccgag cagcgttcca
     2701 aggagggccg aaaggacggc gatgagggcc gtgactttca atccgttcca gatcaacagg
     2761 tagcgctttt cccggatgat gttgtcgacg aagctcttct tgagccgggc cggccaggag
     2821 gggcggagag cggcgccggc ggccggcggc cggtccttaa gcgtcatcat gacctgggga
     2881 ttgacgtaat agccctcgga aaaggcgact ctccgccggc gctcctcggt cgccgtcagg
     2941 tttgaacaaa cgacgtccgc cttcccggat tcgagagccg cgatcagggc ggagaacttc
     3001 atgtcggtga agacgaccgg gcggcccagc ttgaaggcga tccgttcgat cagctcggga
     3061 tcgaggccga cgattttatt gtcctcgatg aatcccatgg gctcccggtt ggccgcgacg
     3121 gccacccgga gagccggcca cccggacgcc cgcggcacct cggttttcac gtagggcgag
     3181 ccgtccggcc ggacccagtg gctgatgatt tcctccaacg tcccatcgtc cctcatttct
     3241 tcgagaaccg cgttgatccg ctcgagcagc cggggattgt ctttatggac ggcgatcgcc
     3301 gcccacgcct gattgtactc ctcgggaagt acggccaggt cgggattggc ccgggccgcc
     3361 tggagggccg tcgtgtacgc ggtgatgaca taatcgacct tgcgcgcttt cagggcggcg
     3421 acggcgtcca tgacgtcgtc gaagagatgg atgccggccc ggggaaaacg ctcccggacg
     3481 agcgcttccc ccgtcgagcc ggtcatcgcc ccgatgacgg cctcggcgat gtccggtttg
     3541 cttcccgtct cttcgggccg gtttccgccg tccaggtgtt ctttcagggt gagggcgtcc
     3601 gccgccgatt ggtaatacgc gtccgaaaag gcgacctgct tggcccgctc ctcggtgatc
     3661 gtgatgccgt cgcagatcag gtcggctttg ccggaggcga cggccgcgat cagcgaggcg
     3721 aagggaaggg gctggatctg cggcttcagc ccttggcggg cggcgaagcg gagggccagc
     3781 tccagatcga tcccgcaggg tttctcctcc cgcgtgtagg aaaagggaat gtcaacggtc
     3841 gtggtggcga tgcgcagccg gccgttctcc ccgccttccg gcaggtcggg gatcgcggcc
     3901 gcgccgtcgt cgccttccca ttttctccgg atctcgtcca gctcgcccgt cgcccggagc
     3961 tcgccgagaa aggcattgaa cgcgtcccgg aggcggacgt cggcgaatcc gaagccgagg
     4021 ttttcaatga aaatcttctc ggccagaacg gccagcgagg gatcgtcctc ccggtacagc
     4081 cggccggcgt tggcatcaaa gatcgccgcc tcgcattttc cggacttgag ggccgcgagc
     4141 atgtcggggg cgttgtccat ccgaaggatt tccgcccggg gaaaattcgc cgtgatgtgc
     4201 ttgtcgtggg tcgagccgac catcaccgcg atccgtttct cggcgacgtc ggccaggccg
     4261 gaaaaggccg gcgctgctcc ggcgacggcc gaatccctca ccgcggccgc gatcccgccg
     4321 cggtagacgg gctcggtgaa gagaacgctc ttggcccgct cctcggtgac cgtgatgcag
     4381 gcggcgatga gatcggtttt cccggacagc agcgacggga tcatcgcccc gaattccatg
     4441 ttgtggatcc ggagctcacg gccgaggcgc ctggccagcc gcgaggccag ctcgacatcg
     4501 aagccgacga cccggcggct gtcgtcgatg aaggaaaacg gttcggtgac ggcgcaggtt
     4561 ccgaaatcaa gaatcccgtt gggcgcctcc caggccattt cgggcatggg cgccggcttt
     4621 cctttttccg ggaaccaccg ggctttcatc tcgtcgtacc ggccgtcggc ccgcagctcg
     4681 gcaagcaggg catcggccgc gtccttcagc tcccggtcgt ccggccgcac ggccatggca
     4741 tactgatcga cggagatcat ctccggcagg atcttcaagc cgggattctg ggcctggatg
     4801 ttgcggagga tcggttcgtc gtaggccgcc gccgtttcct tgcctttcaa gacggccagg
     4861 caggcgtcca gaggcgaagg gtaatacccg aacgtcgctt ccggaaagcg gttaaggacg
     4921 atttgatcgg cgaccgtccc cgaaacgacg ccgaatcggg ccgttttcaa atcttccagc
     4981 gaggcgacgc cggccggctt tccggccggg gaacaagaga ggcaagcgcc ggccagcaat
     5041 ccgccggccg cgatccaggc gaatcgtttc atgcggctat tctatgaatc catcgacctc
     5101 ttggcaattg tccggccgcg ggcctccggc gaaccggccg ggcccgcggt ctccgccggt
     5161 ttgaatttga catccggccg cgggggggta ttaaataaag cgggatgaag aagaaacggg
     5221 ttttaaaagg gattctcgcg cccgcccttc tcctggcggc ggcgatcggg ctcggacagc
     5281 cgacgccgtc ggctgagaat ccgccgcagg agacggaccc cgcccggcgg ccgcccaccg
     5341 actacatgcg ttcgctcttg tcgcaaaaca ccaagccgct cggcgtcaag gtggacaaat
     5401 ccctcgaacg gttgatgcgc cagccggcga tcgcctggac cgcccgggcc gcccgccggc
     5461 atattctgga ctaatccgat gacacgggaa cgcccggcct caatcgaatc aggcacgatg
     5521 gattcaattt tttggattgc accatgaaag accggaagat ctggcttctc gtccccctga
     5581 cgatcctcgt cgttttgggg gttctcaacg tttaccgcca gatcgtttac aaggaaccct
     5641 ccgacggcgt cgtctgggcc atgaaacaag gccgtctgac ggcgatcaaa gtcgacaagg
     5701 acgggccggc ttatctcttc aacttgaaga agggcgacgt tctcttctcg gtcacccaca
     5761 atctcgcccc cggcaagatc atcgtccgga gcaaaatcga cctgatcaaa aacctctggc
     5821 aggtttggaa gcaagggcag aagatcacgt acgagatcta ccgggagggc ggcgtgatca
     5881 cctacacggg gacgtttttc cccgtcggaa agggccccga cattatttat ttctatttgg
     5941 ccctgatcgg cgtgatcacg atcatcatcg ccttggttgt ctttctcaac acctcccggc
     6001 cgttccgccg gaccaacatt cattactatt ttctctcggt cctcctgttc atgttttacg
     6061 tcttctcctt caccggggaa ttcgactggc tcgacagcct gttttacggc ctggataaaa
     6121 tcgcgttcct ggtctttccg ccgctcctcg tccactactt cctgattttc ccccagcgag
     6181 tcaagctgct ccgggataaa cccgggacgg cgtttcttct ctacgcgccc gcgtcgctgc
     6241 tgctcctggc ccggatcgcc ctcttccttc cggccgccgg cggcctcggc gagctccgcc
     6301 ttctccaggc gtattccgcc ctggaaaagc tggatctggg ccatttcgcc ctctatacgc
     6361 tcggcgccct ggcgacgatc gctcacggga cgctccgcca cccgagcttc atcatccgca
     6421 aacagcttaa gtgggtcgcc tacggcctgg gcttcggcgt cgtcccgttc acggccctct
     6481 acgtcgtgcc cttcgtcgcc ggggccgcgc cgagcgtggc cgccgagctg accattcttc
     6541 tccaggcgct catccccctg tccttttcct attcgatctc ccgctacaag ctgatggacc
     6601 tggaagtcat cctcaagaaa gcctccaccc tggttttttc ctacttcgtc ctggccctgg
     6661 ttttcgtcgg gatctcctcg caaacgaaaa tcttctcgga aaacaagatc aacgcccttc
     6721 ttctcggagc cctggccatt atcctgggcg cgaccatgtt cacccccctc cggaccctcg
     6781 ttcaatccct cttcgaccgg gtcttctacc ggcggagcta ccagtaccgc aagacgctcc
     6841 tgtccatctc ccaggagctg agccgggagc gcaacatcga aacgctcgtc cgccgtctcc
     6901 tcgagctgat ctccaacgcc ttgtccctgc gctcgatcgc cctccttctg ccggtcgaaa
     6961 cggggagcgc caccttcacg atcttcagcg cgcacggaaa cgccgaaacg gccgcgggcg
     7021 gacgcctgac gttcgatccc aagatctacg aaaccctggt cgagaacaac accctgtcgc
     7081 tctactcggt gaccgaggac atcaagcttc aaacgaagct cgagagcctc cgggactccg
     7141 gcttcgatca ttttctggcc ctcaagggcg aaagcaagct ggtcgggtgc ctggccatgg
     7201 ggcgcaagat cgacacgtcg tttctgacgc gcgaggactg ggaactgctg acgacgatct
     7261 cttcgcccgt ggctttggcc gtggaaaacg cctacctgta caaccaagcc ggcctccggg
     7321 cctccgagct tgaacggctc aaagactaca gcgagaacat catcgaaagc ctgacggtcg
     7381 gcgtcgccgt cctggatcaa aacggacgga tcagcggctg gaaccgcgtc tgcgaggaga
     7441 cgttcaaccg gaagcgggag gaggttctcg gcgaacgcct cagcgacatc atcggtccga
     7501 gggccttcgc cgcgatcttc cccccggaca cccagtcgga ataccatctc gttggagaga
     7561 tcccgatcgg ccgcccctcg ggcgaaatgc ggatcttcga catcgccaag acgccgctgc
     7621 gggacaaccg ccgtcatccc ttcgggacga tcatcgtctt cgaggacatc accgagaaag
     7681 tccgcctcca gcagcagttg ttgacttcgg aaaagctggc ctccctcggc ttgctttcgg
     7741 ccggcgtcgc ccacgagatc aacaccccgc tcaccgggat ctccagctat gtccagatgc
     7801 ttcaaaagac ggtcggcgac gagcgttttt cccaggtcct gggcaagatc gaagctcaaa
     7861 ccgaccgcgt ctcgcgcatc gtcaagaacc tcctgacctt cgcccgcaat ccggccgagc
     7921 cggggatgca ccggatcgac ctccgggaca ctctccagga gatcatttcc ctcatcgact
     7981 acaagcttcg ggccatggaa atcgagctgg aaaccgacct ggctccggtc gcccccatct
     8041 gggcccaggg cgagcgcctg cagcaggttt tcatcaatat catcctcaac gccctggacg
     8101 cgatgcccgg gggcggacgg ctccgggtgg agctccggga gacaccggtc gaagccgtcg
     8161 tcgtcatcag cgacagcggg acggggatca aggaacagca cttggcgcgc atcttcgatc
     8221 ccttcttcac gacgaagggg gtcggcaagg ggacgggcct cggcctgtcc atcagctatg
     8281 cgatcatcaa cgagcacgaa ggccggatcg aggtccaaag cgaagtcggc cagggctcga
     8341 aattcaccat ctttctgccg aaagatctcg accggcggaa atcgaaaacg agagaatcat
     8401 gagtcacgcc tccatccacg tcatcgacga cgaacccgtc atccgcgacg ttctcgaaca
     8461 gcttctgcag gcggagggat acgacgtcac gctctccgaa tcgggggaag cggccctgtc
     8521 caagttcgcc gaccacggct tcgacctggt cctcctcgac ctcctgatgc ccggccgcgg
     8581 cggcctcgag atcctgccgg agatcaaaaa gatccagccc tcgacgatcg tcatcatcat
     8641 caccgcttac gcctcggtcg agtcggccat ccaggcgatg aagatcgggg cttatgatta
     8701 cgtccagaag ccgttcaagc acggggacct cctcctgacg atccagcggg ccctggccca
     8761 caaaacgctc ttggacgaga acgaccgcct ccgggaggag ctccggaaaa aattcagctt
     8821 ttccaacatc ctcggccgaa gccggccgat ggagcgggtt ttcgagctca tcaaggccgc
     8881 cgcccccacc cggtcgacga tcctggtcca gggcgaaagc ggaacaggaa aggagctggc
     8941 cgcccgggcc atccacacca actcgccccg ggcttccttc ccgttcatcg tcgtcaacag
     9001 cggcgccctt ccgccggacc tcctcgaaag ccaccttttc ggacacgtga agggggcgtt
     9061 cacgggagcg gtctcggaga aacaagggct gtttgaggcg gccgacaagg ggacgatctt
     9121 tttcgacgaa atctccacca tcgggctgga aacgcaggct aagcttctcc gggtcatgca
     9181 ggaccgggaa ttcatgcgcc tcggcggaac gaaaaccatc cgggtcgacg tgcggatcat
     9241 cgccgcgacg aacgtcgagc tcgaggacct gatcgaccgc cagctcttcc gcaaggatct
     9301 ttattaccgg ctgaacgtca tcaagatcat ccttccgccg ctgcgcgagc gccgcgagga
     9361 catccccctg ctggtcaagc attttttaga cgtcttcgcc cgggagaacg gcaagacgat
     9421 cgagggcgtc agccccgatg tcttggaaat cctggaccgt cactcctggc cgggaaacgt
     9481 ccgcgagctc gaaaacctcg tcgagcggac ggtcgtcttc agccgggaca aggtcatcac
     9541 ccgggactgt ctccctgact tgttccggac gccccgggaa gcggcggccg ccccggcggc
     9601 ggaaggcgac gggctggatc ttaaggagca aacgctcgct tttcagcggc gcctgatcga
     9661 aaccgccctg gccaaggcca acggcgtgca aaaaaccgcg gccgcgcttc tcggggtgaa
     9721 gccgaccacc ttgaacgaga tgatcaagag gcttgagatc tcgccggacc gctcctagcc
     9781 tgccttattc gcgagttgaa tatccttaag atcatcatcg aacggataac atctttagaa
     9841 aatatatgaa gaggaccggc tagcggaatt ttccggggac gagcccgtcc ggattgatct
     9901 tgaacttcac cggtttgacg gccgccggaa aggaataaaa cttggattcg gagtcgacct
     9961 cgaggatgtg ccggaccggc cggccgtcct ccgtccaggc cacggtcagg ggaaaaacca
    10021 tcgcctgtcc ggcctgacgg acgaaaatct tcacttcgta gccttcgtcc gccttgagga
    10081 tctggtgcgt caccgaggct tccggaagca catgggaatc gatccatccg cggaaaaagt
    10141 cacggagcga acggccggag gcttcctcca gggcccggat gaactccccc gtccggacgg
    10201 ggcgataggc tgaagaagcc tggaaggcgc gcaggccgcg gtccagcgct tcttcgccga
    10261 tcaaatccgc gaggaggaac aggaccacgg tggatttccc gtagatgatg gcctggaaag
    10321 cccggaaatc aagatagctc agccgggcgc cgagcgtaat cggcccgtaa tccgccatgc
    10381 gcttcgtcca agaaacgctt tttttcagga tcgacggaag cttgtcgttc ccgtgcttca
    10441 tcctcaagta tcgaatggcc gcgtattgag acagcccttc gctcagccac aagtcgtggt
    10501 aggtggccgc gctgacggcg tgcccccacc attggtgggc gatttcatgg gccagaaaaa
    10561 gctcccggta cccggggaga tccaccgggc tcccgaaatc cggaatcccg ggcgccgtgg
    10621 ctcccgtccg cggttcattg aggatgacga aggaagcggg gctttgcccc ccggccgtct
    10681 cggcgacgcg ctggatgata gccagctttt cataggggaa gggcccgaac gtttcttcgt
    10741 agaaccggac gatcgcccgg gcttccccgg ccagcgtccc gcggaggctg cggacgttct
    10801 cggacacgag gacgttgatc gggggcggcg acgggtcctc gtcgaccgcg tcttcccgga
    10861 agcgcccgac gataaaggcc agccccttga ccggcttccg ggtctcgaaa acagaaatcc
    10921 ggttgccgat tttctccagc gacagaaccc ggcggacctc gtcgacttcc tccactccgg
    10981 caagctctcc gttggccacg caaacgtagc ggggagggag gctgatccga aaccgggaaa
    11041 ggaaataatc cccctcggac atggccgggt accatccggc cgacggcgtg tagagatcgc
    11101 cgtcatcgat gaaattcgct ccggcgaatc cgccgtaagc cgccgcggcg tcgctcgtcg
    11161 gcgggggcgg agggagggcc ccgcggtaat agatgtccag ggaagccgat tcccccttct
    11221 ccaagggctg gagaaaccgg atgtaaagaa gacggttcac cttgtcttgg gtgaagaaca
    11281 gctcgcgccg cgaggcgtcg tagaccttga gaacgtccag ggcggagtgg aaatcaaacc
    11341 gcaggttgtc cacccccgga gaggcagctt ccagccggat gcgggccaag gcggaaagga
    11401 aacggcggtc gggctgaaaa tccagatcga tgtcgcagcg gaggatatcg actttttccc
    11461 ccatggtgag gaacatcctt cgttcgttcc cctcgcggat atccggagaa tagaggcaga
    11521 gaagctggtc tggcgagcgg ctccagaaat ggatctcctc ctccgaaaag ggggaatatc
    11581 catatccgaa cccgctccgg gagtcggtcc cgaattcaaa aaccgcctgg tcgctttgcg
    11641 gcaggaccga aagccgcgcc ccggcgatcg ggttgtcgat cgtgaacgag ccgccgtaat
    11701 agcggcggaa caaggcccgg gctttcgcga tctccccggg cggacaagag ccggccgggc
    11761 cggtctcggg ggtgattttg atccggttcg caaaaaacga gggggaaaac cttaaataag
    11821 cggacaccag cggcttgtcc aaattttcgg ttttgtaccg caaggccagt tggtggcgtt
    11881 ccgtctccga cgaaggccga aaacgcagcc gccccgagcc gatgatgatc aagcccgtcg
    11941 ggcggtcggg gatgttgtcg taaaaaaccc aagcgttctc gaatgtcgct tccagatcgt
    12001 catggcggat ttcaattcgg gcggctcgct cgatccggtc cgagggaatt ttcagcttga
    12061 gaaaaatcgc tggattcccg atcacgctct tggcccggat gactcgccgc tccccgtccc
    12121 gggccagctc cagctcccac gactcaaaca aggcttccag gtcgttctga aagacggcct
    12181 gaacgaagag aggaacgcct tccttgtcct cgaagacgtt cgtcgccgga aaaaaaacgg
    12241 cctgggtcat ccgcgaggct tgcctgaggg aatcgtaggc cgcgccctcg gcgtcgcgaa
    12301 tttcggcttc gaaataagct aaataagccg ctcgatcgga cgattcgaag gcctccttca
    12361 gcccggatag gaacgtccgg acgtcggtct gcggcggaag ggattcctgg ccgaacgagg
    12421 cgagagtgaa aacgagaacg agcggaaagg ccgccgccca tccctgcatc cgcatggtta
    12481 aattatagca aaaaacggaa ccgatgatgt ttcgcgttct tctcatctct atcccaacat
    12541 aagcatgaaa catgccaata gaggattccg gccggcggga tcggcttaac cccgaacaaa
    12601 gcccggccgt aaaccgcaaa atcgttggaa acaaaaaaga aaaaaacctc cgcgatttat
    12661 acgatgtaat gaatttttac acggccgatc ccttcaccat ccgaaaaagg cgcgttttgg
    12721 atcccccgaa aacgatttac acgggaagtc ttcttcttta atctaaagac gccgcggggc
    12781 gatccggaac catgccggaa ccggccctgg aagggagaca cccgtgaaag ccatgctcat
    12841 cacccgaatc ctcgacgcct gggagagcgg cgagccgctc gaaagccgcg atctccctct
    12901 ccccgtcccc ggtgaagggg aaatcctcct ccgcatcgcc gcttgcggcg tctgccacac
    12961 cgagatcgac gagatcgagg ggcgaacgcc gcctccccgg ctgcccgtca ttcccggcca
    13021 ccaggtcatc ggccgggtcg ccgcccgcgg tcccggcgcc cgaaggttca agctcggaga
    13081 ccgcctcggc gtttcctgga ttttctcggc ctgcggcaag tgccgtttct gccttgaggg
    13141 ccgggaaaat ctctgcccgg aattccgagc gaccggccgc gacgccgacg gcgggtacgc
    13201 cgagtacatg gtcgtcggcg agtcgttcgc cttctccatc ccctcccttt tcaccgacgc
    13261 cgaggcggct ccccttcttt gcgccggggc cgtcggctac cgttcgttga agctcgccgg
    13321 gctgagggac ggacaagccc tgggcctgac cggattcgga gcgtccgggc atttggtcct
    13381 ccagaccgcc cggcatttgt tcccgcggac gcgcttctac gtcttcgccc gaagcccgga
    13441 ggaacgcgtt ttcgccgcgg agctgggcgc ggcctggacc ggcgaggcct cggacacgcc
    13501 gcccgaggac ctggacgcga tgatcgacac gacgccggtc tggaaatccg tcgtcgaggc
    13561 cttgcggtgc ctggccccgg gcggacgcct tgtgatcaat gccgtccgga aggaagacgg
    13621 ggacaaagac gcgctccggg cgctcgatta cgccggccac ctctggcggg agaaggaaat
    13681 caagagcgtg gccaatgtga cccgggcgga cgccgtcgaa tttctcaggc tggccgcgga
    13741 aatccctctc cgccccgcgg tgcaggaata ccccctggcc gaggcgggcc aagccctcgg
    13801 cgaaatcaag cggcggcgga tccggggcgc caaggtcctc cggatcggct gaaacccgcc
    13861 ggaggcgcta tttcccggct ttgatctcgt cccagatctt cacgtaaagc gcgttctttt
    13921 cccccagatc ggccaggatc tcgcaatggg ccttgatctc gggagtcaga aaaatccccg
    13981 gattccccgt gatctccgcc ttcaacaacg gataggaatc cttgttcggg cacaggtact
    14041 ggatgaactc cgtgttttcc gcggccacgg ccggatcatg aaagaaattg atgtaagcgt
    14101 gggccagatc cggctcctgg gccgtcttgg cgatcaccag gtcatcgcag gaaatggccc
    14161 ctccctcgcg gggcatggcg aaggcgatgt cctcgttttc ggcctgcact tggaggatgt
    14221 cgccgctgta gccgtgcacg agaaggaatt cgccggaggc gatccccgtc ttgtattgct
    14281 cgttctcgaa cttggccagg ttttctttcc aggccaggac aacgttcttg gccgcgctca
    14341 gctcctcggg attcgtcgag ttgaggctgt agccgaggaa tttcaacgcc gccccgatcg
    14401 tttcccgcat gtcgttgagc atgaccatcc ggccccgaag gtcggaccgg ttgaacatcg
    14461 cccaggtcgg ctcgaaatcc ttgaccttgc tcttgaggta ggcgatcccc gtgttcgtca
    14521 gcatgtaggg cacggaatag gccatggttt tgtcgatggc gatggccagg taatccggat
    14581 cgacgcggga gaggttcggc agccgggagt ggtcgagggg aagaagcatc ccctggctct
    14641 gcatcaggct gaccatatag ctcgtcgggg tgatgacgtc gtagcccgtg gctccggcct
    14701 tcagcttggc gtacatcgcc tcgttggaat cgaaggtgtc gatgacgact ttgcagttgt
    14761 tctcggcctc gaaccgggcg atcaattcgg gcttgacgta atcggaccac gtataaacgt
    14821 gcaggattcc cggggccttc gctccgcccc cgcatcccgc ggccaataag ccggccgcgg
    14881 cgagaaacgc tcccagagcc gaccgtccga acgtgcgcat cttcattgtc ctccctccaa
    14941 agctccggat ttggataaac gctggctgac gagcacggtc gtgaacgtca gggccagcag
    15001 cagggttgaa agggcgttga taagcggggt ggcgccgtgc ttgatcatgc tgtagacgcg
    15061 gatcgggaga gtggtcgatc cgggaccggc gacgaaaaag gtgatgacga aatcgtccac
    15121 cgacagggtg aaggcgatca ggccggcggc ggccaagccg ggcgcgagca tgggcagcag
    15181 cacccgccgg gtcgccgtcc agccggtcgc tcccaggtcg cgggcggcct cgacgaccgt
    15241 gaaatcaaaa tcctggagcc gtcccaggac gaccagggcg acatagctga tgcagaacgt
    15301 cacatgggcc aggatgatcg tccccatccc cagcttgacg ccggcggcga cgaaggccat
    15361 caggaggctg atccccatca gaatctcggg aaccaccagc ggcgtgtaaa tcagcgtgta
    15421 atggacgcgt tgaaaccgcg actttgagaa acggtgaagg gccagggcgg acgtcgtccc
    15481 cagaaggagg gagacgaacg tcacgatcaa ggcgatgacc agggtgttgc gggcggccgt
    15541 ccagatggcc ggctcgtgaa acagccggac gtaccacttg agggaaaatc ctctccacgc
    15601 cccgccgaag cgggaggcgt tgaaggaatc gacgacgaga ataaggatcg gaagatagaa
    15661 aaacaccagg acgaggccgg tgacgatgaa gggaaaccgg ctgcgcttca cggcttcccg
    15721 cctccttccg gcggctggat ccgggccttt ttcccctgga gggcgagaac ccctaaaagc
    15781 ggcgccagca ccgccaggat gagaacgatc gaaagccctc cggccaaagg aagattccgg
    15841 tcgacgaaga cgcgctgggc gatcttgttg ccgagcattt cgcttccggg tccgccgacg
    15901 atgtccggaa cgatgtaaga tcccagcgac gggatgaaaa cgaccaggac ggccgtgacc
    15961 aaggcctgcc ggatgcccgg gagaaagacc ttccggaaag cctggaaagg ccgggctccc
    16021 aagtcgcggg cggcctcgag gagccggtaa tcgaatttct cggcggccgc gtaaatcggg
    16081 aggatggcga agggaagaaa cgtgtaaacc gtcaccagga tgaccgcccc cagattatag
    16141 agaagctggg aggagggatc gaccaggccg cagaaaacca gggttttttt gatgaggccc
    16201 tcgggatgga gaatcgactt ccaagcgaac acgcggatca ggaagctcgt ccaaaacgga
    16261 acgatgacaa gcatgaggag aagattccgg cggcgctccg gggcccgggc gacggcatag
    16321 gccgtcggga cggccaggag caggcagaac gccgtggaca ggaagctgac ccacagcgtc
    16381 cgccaggcga tcgcgagata ctggggatcg gccaagcctt tgatcgtggc cgtcgtccag
    16441 ccgcggccga tgcccccgaa gggatcggcc gggcggaagg cgaggatgaa gaccagggcg
    16501 gtcgggatga gaaaaaacaa cgacagccaa aaaaacgacg gcagggtcag gaagaactcc
    16561 tgtccccggc gccggaggtt tttcatgccc ccggctcccg gcccgtccca gccgcggcgg
    16621 tcatgcctcc cccacttttt ggggcggggc ggcgacgagc ttttcatcgt cccgccggaa
    16681 gccctcgagc atgaacccgt cgtcgccgtg ccagaacacc cagacatcgt ccttccagcg
    16741 gatcggcttc tcgtcgagaa aaaaccgggc gtgctggcgg agaacctgga ggcggtgctc
    16801 gccggcccgg acccagtact tcgtcgtcgg accgaggtag atcacatcct cgaccacggc
    16861 cggaacggcg ttccgcttcg gcttcggctc gggccggtcg cgcgaaatct ggatcttttc
    16921 cggccggatg ctcaggaaaa tcttatgtcc gtcggcgatc tgtttgtcgt tgtaaacggt
    16981 cacgtcggga agtccctcga tccggaggcg gctgtactcc ggttccggat gatcggcgac
    17041 gacgccctcc aggaagttcg tgtcgccgat gaaggcggcg acgaagcttg tccgcggcgc
    17101 ctcgtagatc tcagccgggg cgccgatctg ctcgatccgg ccggcgttca tgaccgcgat
    17161 ccggtcgctc aggctcatgg cctcgctctg gtcgtgggtg acgtataaaa acgtgatccc
    17221 gacctggtcg tggatcatgt cgagctcgat gagcatcctc tggcggagct tgaggtcgag
    17281 ggcggccagc ggctcgtcca agaggaggac gccgggcttg ttgatgaggg cccgggcgac
    17341 ggccacccgc tggcgctggc cgccggacag gagatccggc ttcttgccgg cctggtcctc
    17401 caaccggatg aggcgaagca tcgtctcgac ttcgcggtcg atctcggcct tcggcttctt
    17461 ggcgatctgg agaccgaagg cgatgttgtc ccggacggtc aagtggggga acaaggcata
    17521 gctttggaag atcgtgttga ccgggcgccg gttcggggga agatcggtga tctcccggcc
    17581 ggcgagaaag acttggccgg cgtcgggcgt ctcgaacccg gcggcgatgc ggagcatcgt
    17641 cgttttgccg cagccgctcg ggccgagaag ggcgaaaaac tcgccccgct tgatttccaa
    17701 ggacacgccg tcgacggcct ggatggctcc aaaccgcttg gagacgtttt taaactcgag
    17761 aaattcggcc acggcgctcc ttctgtgagg ggcattatgg aacaatcgtt ctggtttttc
    17821 aagaacgaat atgctataaa taccgccgac gcaaatgaat gtcatttcga tagttatcca
    17881 gatttttgtc ctcctcttcg ccatcacggt gcacgaggcg gcccacggct ggacggccaa
    17941 gaagatgggg gacccgaccg cctacaacat ggggcgggtg accttgaatc ccatcgccca
    18001 catcgatccg ttcggaacga tcctcctgcc gcttcttctc tcgctggccc gcctgccggc
    18061 gttcggctgg gccaaacccg tgatggtgaa tccccgcaat ttccgcaatc cccgccggga
    18121 caacctctgg gtctccttcg ccggaccggc ggccaacatc tccgtcgccc tcgtctcgct
    18181 catcctcctg ttcctgatga agctgacgat tcccggcgtc gggcggttta tccaggcctt
    18241 gatcaacgga ttcttccggc agatgggctt cctcctgccc gtcctctctc gaggattcta
    18301 tccgctggag ggcctggccc tcattctgat catgaccgtg ctcatcaatt cttatttcgc
    18361 ccttttcaac ctcatcccgg ttccgccgct ggacggatcc ggcgtgttga tggggcttct
    18421 ttcggaccag gccgcgttcc gctacgatcg gatccggccc ttcggtttca tcatcgtcct
    18481 cttcctgatt tatatgggtt tcctggacat cctgatgtgg ccggtgaagc tcatgattgt
    18541 gattgtgctc ggatgaatcc cacccccgat ccccgcaaaa tcgtcctcag cggcgtccgc
    18601 ccgaccgggc cgctgcacct cggcaactac gtcggcgccc tccgcaattg gattgccttc
    18661 caggacctct accggtgctt ctacgctatc gtcccctggc acgccctgag ctctgaatac
    18721 gccaacgcca aggcgatccg gggctggtcc tacgaggtcg ccgtcgactg gctcagcgtc
    18781 ggcctcgacc cggaaaaatg cgtcatgttt ctccagtccg aggtcaagga acacgccgag
    18841 cttcatcttc tcttatcgat gatcactcct ctgccctggc tcgaacgggt tcccaccttc
    18901 aaggaacagc tgaaagagct ccaggaaaaa gagctgaaca cgtatggttt tctcggttat
    18961 cccgtccttc aggccgccga catcatcctc tacaaggccg atctcgtccc cgtgggcgag
    19021 gaccaggccg cccatgtcga actgacccgg gagatcgtcc gccgttttaa ccgcctctac
    19081 ggcccgattt ttcccgaacc ccggacgatg ctgacggaag tgccgcggct ggccggaacc
    19141 gacggacgga agatgagcaa atcctacggc aacgccatct acctgaagga ttcggccgag
    19201 acgatccggg cgaagatcct cccgatggtc accgataccc ggcgggtccg ccgaaccgat
    19261 cccggcgagc ccgacgactg ccccgttttc accctccaca aggccttcgt ccccaaggag
    19321 gcgcgggagg acatcgcccg cggctgccgg acggccggga tcggctgccg cgactgcaag
    19381 gccgtcctcc tggagcattt gttccgtttc ctcgaaccgg tttgggagcg ccgccgcgcc
    19441 ttagaaaaga acccggagag aatctgggag attttcgaag ccggcaacag ccgggcccgc
    19501 cgcgtcgctc aagccacgat ggccgaagtc cgggacgccc tgggatttta ggctcgaggc
    19561 ccggacgcat ggtcgacccc ggatatccca acgaggaagg cccgctcgag aagccggccc
    19621 ccgaagagga atccgccgtc gaggggggcg gggacggcta ccagatcaag ctcgatattt
    19681 tcgagggacc gctcgacctt cttctcttcc ttattaaaaa gaagaagatc gggatcgagg
    19741 atatccccct ggccgtgatc acccgggaat acctcgatta cctccacggc cgggaacgga
    19801 tcaacctcga ccgggaagcc gaatttctct tgatggccgc cctcttgatc tacatcaaat
    19861 cgcaaatcct ccttccccgg gagcagcccg tcgagccgga catcgaccct cggaaggagc
    19921 ggcccgaccg aaccgaggat tacgagcggg tcaaggtcgt ttccgccgtc ctccgggaca
    19981 aggaggagga gcagtcgacg atctggcggc ggacggccct caacgttccg ctgcccgagg
    20041 aggatctcga cttgatcgag gtctcgctgt tcgacctggc tgaatcgttt ttcgccatga
    20101 tgaagcggaa acaagccgtg ccgcagcgga tcctcaagac caaggaaatt tcggtcgcgg
    20161 acaaaatccg ggaaatcgtg agcgccctgc gggagcacgg ctttctcgat tttcttgaat
    20221 acctggacgc ccaggagacc ctcgaggagg ccctggtcgc ctttttctgc ctcctggagc
    20281 tgatcaaggc ccgccgggtc gtggccgtcc aggagcagct gtttcacacg attaaggtct
    20341 ggcttcggaa agagccggcc ggcgaggacg cctgatggac gatttaaaga acattctcga
    20401 agccttgatt ttcatctcgg tcgagccgtt gacgatggac aagatcaagg aagtgcttga
    20461 tggaacgccc gaggaagaga tcctccaggc ccttctccgg ctcgggacgg aaatggagga
    20521 cgccccccgt cccatccgct tggtgcagac gggcggcggc tgggtgttca tgaccaaacc
    20581 ggcgtatgat ctccacgtcc ggaagcttct tcagatcgag cgccgcaagc ggctgtcgtc
    20641 ggccgccctg gaagccctct cggccgtcgc ttatcatcaa cccgtgaccc agaccgacat
    20701 catgaacatc cgcggcgtcg attcgaccca tgcccttcac accttgctcg agaacaagct
    20761 catcaagatc tccggccgca aggacgcgcc gggacggccg ctgctctacc ggacgacgga
    20821 gaaattcctg acgtatttcg gcttgaattc cctcgaggag cttccccggg agacggagct
    20881 gaagaaaatc ctcgaggaag gcaacgcctg aggtttttca tccgggccgg ccgtcccgat
    20941 ttggcctttc cccggctgta tccgtccgac cgccgtcgcc cgcgggaaag ggagagacga
    21001 tccggccgac aattttcatg gattcctgcg ggcgatcgcg ccgaggcgga gaggaaagaa
    21061 agccccgcct ctcccgccgg acggacagga gcgtcaagga cgagccgcgg cgcctcggcc
    21121 cgcttttccc gggacgggag ggacttcatc taagttcaat ccttatcttg acttagcgat
    21181 tgacaaaccg ggttgaatcg tttagactta accatctttt tcaccctttt gattaacatg
    21241 gttaggaaat catatccgtc ttttatgctc gcgggagcaa attccatatc cgggaaaacc
    21301 gggagcggag aagccacggt tttgccggcg ggcggatccg ccggcgccct acccatcaag
    21361 gagaccccat gaccgaagat gcaaagcaga acaacgaaaa aatctcggcc gaggattacg
    21421 agaacctgct ggacaagtac caattcagct cccaggaagt cagcccggga aaaatcgtcg
    21481 ccgggcgagt cgttaaagtc ctgcccgccc acatcctggt cgacatcggc ctgaaaagcg
    21541 agggactcat cccgatcgaa gacttcacgg aaagcgccga cgcccggatt ccctccgtgg
    21601 gcgacgaaat cgaggccgtg ctggagcgga ccgattccaa ggaaggatac ttcatcctgt
    21661 ccaagcgcca ggccgacgcc cgccgggccc tggagcttct ggaaaaggcc tacgaagaag
    21721 gcgcctccat cagcggccgg gtcatcgaca ggatcaagaa cggctatacc gtccatgtcg
    21781 gtctcaaggc tttcatgccc gattcccatg ccgagctccg gccgctcaag gacccggcta
    21841 agatcgtcgg ccaaaccctc aaggtcaaag tgatcaaata cgaccgcaag acggaaaacg
    21901 ccgtcgtctc ccaccgggcc tacctgatgg aagagcggga caagaagaaa aagatcgtct
    21961 tcagccaaat ccagaaaggc cagaagctaa aaggactggt ccgctccctg acgagtttcg
    22021 gagcgttcgt cgatatcggc ggagtggaag gcctgcttca cgtctcggac atgacctggg
    22081 gaaagatcgc caacccgcac gacctgttca agatcggcca ggaagtcgag gttctcgtcc
    22141 tcgatttcaa cgagaaggac gaaaagatct ccctggggac caagcagctc acccctaatc
    22201 cttgggacaa catcaccgac aagtacaaac ccggcgaccg gatcgaggga aaagtctcgt
    22261 ccctgaccga cttcggggcg ttcgtcgagc tggaacccgg catcgaagga ctcgtccaca
    22321 tttccgacct gacctggtct cggaaaatga ttcatccaaa aaagctcctg aatctcggcg
    22381 acaaggtcac ggtgtccatc ctggacgtca atccgacctc ccggcgcgtc tccctcggcc
    22441 tgaaacaaac ccagccccat ccgctggaag agttgaaaaa gcgtctcgtc ccgggaaccc
    22501 ggatcaaggg gcggatcacg tccatcactg atttcggagc gtttctggaa gtcgaacccg
    22561 gcatcgaggg cctgatccat atcaccgaca tctcctggga aaagatcaag cacccctcgg
    22621 ccaagctgaa agtgggcgac gaggccgagg tcgtcctcct gaacatcgac gtcgagaagc
    22681 agaaagtctc cctcggcatc aagcagctcg agggcgacat ctgggaggaa ttcttcctcc
    22741 ggcagaaagt cggcgacctg ctcaacgtca aaatcgtccg gatcgccgaa ttcggcgtgt
    22801 tcgccgagat tctccccggg atcgagggcg ttgtctttaa ttccgagatc gacgagaaaa
    22861 aggtcgagaa cccggccgag gtcgtctcgg tcggcgatca gaaaacggcg aaaatcatca
    22921 agctgaatcc ccgggataag aagatctccc tcagcttccg ccaggcccag agcgacctgc
    22981 agaggcagga ataccagaag tatcaagaaa cccaggatga ccggttgacc ttcggcgacc
    23041 tgatcaagga ccagctccgc cagctgcaga acgccaagaa atcaggcgcc gaaacggagg
    23101 agaaacccaa tgattaaggc cgacctcatc aacaagattt ccaaggagct gaacatcccc
    23161 aagcaggaag cggagaccgg cgtcaatatg ttcttcgaga cgatcaagga agccctgctg
    23221 gccggggagg agatcgaggt ccggggcttc ggctcctttc ggttccggaa ccgcccgccc
    23281 cgctccggcc gcaacccgag gacgggcgac ccggtcaagg ttccgcccaa gaaagtcctc
    23341 tatttcaagc cgagcaagct gctcaaggaa ctgatcaaca agtaacatgg aatatatcgc
    23401 cgctccctgg cgggagcctt acatccggag cacgtccaaa aaagaaacgg gctgcgtttt
    23461 ctgccgggcg gcccggaccc gggacgacgc gtccgccttc gtcctcgacc gcggccggcg
    23521 ggcgttcgtc ctgatgaaca aatttccgta cctgcccggc catctcatga tcgccccgta
    23581 ccgccactcg gcctcgctcg agcgcgcctc ccgcgaggtc accggggaaa tggacgagct
    23641 tctccggagc tgcctgcgcg tcctaaaaaa gcactaccgc ccgcaggggt tcaacatcgg
    23701 cctgaacctg ggccgggcgg ccggcgccgg cgtcatcgat cattttcaca tgcacgtcgt
    23761 tccgcgctgg accggcgact cgaatttcat gcccatctgc gggcggacga agatcgtcct
    23821 cgaggatctc gagacgacgt acgcccggct ggcccctctt ttccgccggg aacggcggaa
    23881 cactccctca aaatcatgac ccccgcgtcc ccgaggtgaa ccgatgctca aatccatgcg
    23941 tgaaaacgtc aagtccctga agtgggttct ctggcttgtt gtcgccgcct ttgtcgtctc
    24001 gatcttcttc atctggggcg cgtccggaaa actcaccggc gacgacgccg ggaacaccct
    24061 ggccgtcatc ggccggaaaa aaatcagcgg cgatgattat ctccaggctc ttcgcaaccg
    24121 gatcgaagcc atcaagtcgc aataccagga aatcgaccgg gcattcatcg agcagctcaa
    24181 ccttccccag caggtcctgg agcaactcgt cgagcagact cttctcgtgg aacaggcccg
    24241 ggcgatgcgg ctgcgggcga gcgacgagga gctccgcgac cggatcacgt ctattcccgg
    24301 cctccagcag aacggcaaat tcgtcggcta cgaaatgtac aagagggttc tccagtacaa
    24361 ccacatcaac ctcagcgagt ttgaacacag cctccgccag gacatcgtcc tggacaagat
    24421 gatccggctg ctgacggccg ggctggccgt cactcccgac gaggtctggt ccgattacca
    24481 aaaggcctcc gactcggccg gcatcgatct gctgatcctg gagacgtcca aggtctccct
    24541 cgacgaggcg ccgtcggcgg ccgaggtcca ggctttcttc gaggcccgca agggcgctta
    24601 tctcatcccg gagaaaaggg aaggcctggc cgtcttcttg aaagccgagg atctgaaaaa
    24661 ggaaatcgag ctgtccgaaa aggacatcga gaactattac caagccaatt tgtcccggtt
    24721 caccaacccg gaatccgtcc gggtcggccg gatttggctg ccgttcgagg ggaaagacaa
    24781 agcccttgtc gaagccgagg gccggcgcgt tctcgaacgt ctcggcgcgg ggggcgattt
    24841 cgcggccctg gcccgggagc tgtccaagga cgccaaggcg tccggcggag gcgattatgg
    24901 tttctatgat tggcagtcgc ttccccagcc cgaacgggat gagattcacc ggctgacatc
    24961 cggccagacc tcggacctga tcgccgaggc ggacggcctc gccgtcatcc gggtgacgga
    25021 aaaaacggcc gcgacgacga ccccgctggc cggagcctcg gcccagatcc ggtccatcct
    25081 tctggacgaa aaagcccgtc agctcgccga cacccggatc gccaagatcg agaaggaggc
    25141 caagtcggcc cggagcctcg agacggccct ccggaaagcc ggcctgaaag gggaatccac
    25201 cgggctgttg aaaaacggcg aggcttggag cgacaacgat ccgtccggcg ccgtcggcac
    25261 ggccctcttc gggctcaagg agaaagaagt ttcggccccg gtctacacct acaccggcgt
    25321 cggtctggtc gagctgcgga aggttgaggc tccccggccg gcggcgttcg aggaagtcaa
    25381 ggcggacgtc gaaacggccc tggtcaacga gcgcaaaatg gaaacgtccc gggcccgcct
    25441 ggccgctctc ggacgtcgtc tcgaggagaa aaactgggag gatatcgcgg ccaggaacgg
    25501 attcgagttc aagaccgtcg aggcccacaa gcgcgaccag tatatcgcca tcgtcggcga
    25561 aagcaagacg gccgacgacc tggccttcac ccttcccctg aacgaggcca gcgcgccgtt
    25621 cgatttcgag acggggacgg ccgtccttcg cgtgcgcgac cggaagacgg tgacccggga
    25681 ggaattcgaa aacgtgaagg aaacccaaac ggcccagatc ctgaaccaga aaaagaacat
    25741 gttcctccag gcctacctgg ccaagcttaa acaggaaaag ggcattcaga tcaagtacga
    25801 caagttcctc cagctcaccc aggacgtcct ctcccggtac gaatccgcca agcagtaatc
    25861 ctggcctgga atcggggacg gattcggatt tcgcgccgat acccgcgaat cccgccgggt
    25921 gccgttttct ctgctctgaa ttccacccct gtctcccatg tccttcgttt gtcctccgcg
    25981 gtggacaaca aaaagccgga cgaacggaaa acggagcggg ccgaaaaaaa gcgcgggatt
    26041 ccggggacgc gcgtatttaa cggatattca tgcgtttaga aattcgagtc tgaattttcg
    26101 tctttttggt acgattcttg ccgattctct ttttggaacg gctcgaaaaa cgccggccaa
    26161 ggagaagcga gatgaagacg aagacgatga cggccttcct gatcctggcc gcgacggcca
    26221 tgacgacggc ggcctgtttc cgttcctacg ggcaccgccg ttatcccggc acaccgaggt
    26281 ttgcgtccaa tgactacgcc tatgtccagc ttctccggcg cgagccccgc cgtgatcatg
    26341 tccggttggg caaggtttgg ttcaggcccg aaccttggat ggaccgttat gaagtcaaac
    26401 tcattctccg ggaaaagacc gctcggatgg gcggcgacgc gctgatcatc gtggccgacc
    26461 ggtatccgcg cggcgccgcc gctcgccgct accggagccg ggccgtcggc gaacgctgga
    26521 tcatcggctt cgtcatccgt taccggcgct agcccgcccc gcatgagcct tctcggccgc
    26581 gtctgacctg gacgattccc gagtcaagcc ggcggcaaag acaaaacgcg gcgggggatg
    26641 aatcaccggg cgcccggcgg acggttttca acgaagccaa gagcaggctt gccgggattc
    26701 tccgacaggc ggtctccaac ggggccggcg gaacataagc cctttccccg gacaacaacc
    26761 gtcccgaggg cggccgccgg ggacgacgat tcccgcgtct ttgacaaccc gcgagtccgt
    26821 cggctaaagt agaggcctgt tgaaggaggc cccgatgaat cccgtgaaaa acaagctcta
    26881 cgctgcggcc gagaaagccg tcacccaatc cctccggatc aaggccggag aacagttcct
    26941 cctggtcacg gacaaggcca agatggacat cgccgaggct ttcgccttct gggccaagaa
    27001 gtgcggagcg gagacgacga cctatctgat gacggaaacc ctccggccga tttccgaacc
    27061 gacgaagctt tttcggatgc tggccgaaag tccaaaggtc atggtctaca tgctcgacgc
    27121 ccggatcgcc gaaaagccgt ttcggggcta catggttcaa gccggcgaga cccacggccg
    27181 gctgtgcatg atgcccggga tcacggtcga catgatggag cggctggtca acatcgacta
    27241 tttcaagctg gccgaaaaaa ccaagaggat catgcggctt ctcaaggacg ccggagacgt
    27301 ccggatcacc aatccggccg gaaccgacat ctccttcagc gtcaaaaagc gcggctggca
    27361 catcgacatc ggcgacattt ccaagcgcgg ccgccacgga aatctcccgg ccggcgaatg
    27421 ctacacctgc ccggtcgagg agacgtttac gggcaaggcg gtcatcagcc tgatcgacga
    27481 caagctcggc cgcggggtga tgcatttcga gcgcgggaag cttgtctcct cttccgggag
    27541 gggcatcgag gcggttctga agaacatcgg cgaagatccg accggccgga tcatcgggga
    27601 attcggcgtc gggaccaaca ccggggcccg catctgcccc aatatgctcg aggccgagaa
    27661 agccttcggc acggtccatt tcgccgtcgg cgattcctac gggatcggcg tcaacaagag
    27721 ccgccaccac tacgacgcct tgatcgaaaa ggcctcgatt tacgccaagg gccgctggat
    27781 cgcccgggac ggccgctatc tcgtttgact tcccgtccgg ggccgggcta tcctctctcc
    27841 cggacctgac gccggaatta cataaaccgg gggagaatct ctcttgaatc acagcgaaat
    27901 cgtcagcttt atctggggcg tggccgatct catccgcgac accttcaagc ggggcaaata
    27961 ccaggacgtc atcctgccgt tgaccgtcgc gcacatcaag aaccgcttcc ggacttaagc
    28021 cgataatttc ctcttgacgt ccggcttgtt cttctttatt atcacctggt cataaacgac
    28081 ggcagaattc catgatccgc aaagaggaga ggcatgagcg tctccgcgag gagatcgagc
    28141 ggaatccgtt tgtcacggac gaggagctgg ccgccgccct cggggccagc gttccgacca
    28201 tccgcctcga ccgttcgacc ctgtccatcc ccgacgtccg gcgccggacc cgggaaatgg
    28261 ccggccgcta cttcggcgtc tcccggtcgc tgggcgcccg cgaccacgtc ggcgaaatga
    28321 tcgagatcga tccggggaaa agcggcctgt cccttctgga aaccgagtcc tggatgtgcc
    28381 tggagaaatg cgacatcgtc cgcggccacg tccttttcgc cctcctcaac tccctggccg
    28441 cggccgtggc cgaggctcca atcgccttga ccggcgaagc ccgcgtcgat ttcctccgtc
    28501 cggtccgggc cggcgcgcgc ctgatcgcca aggcccgggt cgagaagaag atcggccgcc
    28561 gggtcttcat cgaggcggtc gcccgctcgg gggagaacat cgtcgcccgg ggacgcttcg
    28621 tcgtccacgg gctgaaccgg gagacggccg cccatctcca tcttttaaag gacggcgagt
    28681 aaatgccgaa aggaacgatc atgaccgaaa tcggaatccg cagcatcgga acgagcgtcc
    28741 ccaaaaaaat cctgaccaac gccgatttgg aaaagatggt cgagaccacc gacgagtgga
    28801 tcgtctcgag gacggggatc aaggagcgcc gcgttctcgg ccccgacgaa aagctccggc
    28861 cgcatgtagt ggccgcggcg aaaaccgccc tcgagcgggc cggccttcag ggaaaggacg
    28921 ccggtttcat catcagctcg acggtcatct ccgaccggtt ctgcccggcc cagtcctacg
    28981 aaatcgccca cgacctcgga accgagcggg ccctggcctt cgacctcaac gccgcctgca
    29041 gcgggatgat ctacgggttg ggcatcgccg agagcctcct tcaaacccgg gatctcgcct
    29101 ggggcttggt cacggccgcc gagcagatga ccacggcgac cgattacacc gaccgagccg
    29161 gctgcattct cttcggcgac ggagcggccg cggtcgtcgt gaccaaggaa cggccgatgc
    29221 accgcctcct ggccaccgag ctcggctcca tcccctccct cgtcgacgaa gtccagatcg
    29281 gcggcgccaa agaccttttg gccggacgga cgaaaaactt ctggttccgg cagaacggat
    29341 cgaccgtgat gaaattcgcc gtccggatca tgcaggacct ctgcgagcgc gtcccccgca
    29401 aggccggggt cgacatcaag gacgtccgct acgtcatccc ccaccaagcc aaccagcgca
    29461 tcctcgacgc cgcggccaaa ggagttcccg ggccggcgga attcatctcg gtcatcggga
    29521 aatacggcaa cacgtcgtcg gcctcggtcg gcttggccct tcacgacgct tgggaccgtt
    29581 tccaaacggg cgacctggtt ctcctcatcg ccttcggagg cggactcagc tgggccggcg
    29641 ccctcatcga gtggtgagcc ggccccatct gtcaggaagc ggacagcccc tggagggcgt
    29701 tcccttcccg gccgaacgga tcttgcccct gaactgatta atccggatta ttcacgattt
    29761 gaatcatccg gatgaaaaag gatttcgcaa ggattcgacg agcaaccgct cgacggcttc
    29821 ttgaagcgcg gcccggaacc gttccaccgt ctcgtttatg tccccgccga ggaaatcccg
    29881 gatatagagg ggcaggccga cgttcacccg gatcgttccc ggaatccccc aggcccgctg
    29941 tgtgccgaaa atcgacaggg ggacgacggg gatatccaat ccctccccgg ctttcatccg
    30001 gaaggcgatg tacgggatcc cccggccgaa cctccggacg tagggattcg ggtcgtccgg
    30061 gtggacgcgg ccccggccct gcatcgagac gaccgcccgg ccggcccgaa ggtaatccat
    30121 gaaaagagag acggccgtca ggttgctccc ctcgtacatg tcggccggga tcgtcccgac
    30181 gcgggcgatg ttcgtcgaga cgaagcggac gaaaaccgtc ttgagcggcc ccagcagccg
    30241 gtcaagaacc aggccgtaat tcttcaggtg gcggcggagg tgcttgcgga tcaggaagtc
    30301 cagctccccg cggtcgaaga gcatccggtt ggcgttgaaa aacaagggac gggagacgat
    30361 ccgaaagacc gcggctggat ccttgatcgc tccgcagtga ttgctgacca ggaggacggg
    30421 ccccgtctcc gggatgcggg cggcgccgcg gcaagccagg cgcttgggaa acaaggcggc
    30481 tttcgtcagc cggtcgatca cgggaaggag gaggcgaagc tcttcttcag gggacggcat
    30541 ggcggatccg cc
//
LOCUS       DAOOXH010000020        29668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_1340_length_29668_cov_8.585047, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000020 DAOOXH010000000
VERSION     DAOOXH010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 29668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29668
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1340_length_29668_cov_8.585047"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2201)
                     /locus_tag="PKV64_04070"
     CDS             complement(<1..2201)
                     /locus_tag="PKV64_04070"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HPN16292.1"
                     /translation="MNGRLRPVLNRVSPVRPGEERLAGFLCAHFFFLMAAIYVSKVSK
                     TSLFLGLGLTKTSLPLVYFISAVLSGLFAAWNTKKIRALDRRAYIAWSQLFFAVGTVA
                     FWLLFGLGGRLVPALYWFWFELFVLASVSQFWVVVGSICEPRRAKQLVGFFVGGGLAG
                     GIAGSVLVVLLSSRLPARHLILLGLVFYLIAMTFIRAALRSAPGREEELGAGPVNYSD
                     GFRTLAASPYLRLLSGLIIVALAASTMIDFQFNWAVKNAFPLEARRTDFLAAFSVGVL
                     VVSWILNSVFAHRAIQALGVRAALFVTPLILALGSAAVFIIPAAGITAWAVLVKGSDK
                     ALTHTFFQATKQLLYLPLPEEVKVKAKMTIDIFVSKCGEALASLLQVVFAVILAFDAA
                     RMSFVTIGIIGLWLVILVRIGRNYLPAIRANLVPSRPDAERLVREQARADTAKRLVDL
                     LESRERSSVLYAYNLFDLLKAERMTPELRQILSEKSAAVRASALDTLLDLDSEMLGPE
                     WAEGEDPAALDVIVREVLALDSYQDVMRRPFGEVAASASPAREVDQMELAKVLGLMPA
                     GAPLTDYLPRLLKHESPGVVHYALESAARLKKRDWLPLIVPHLGHPAVRDRAAETLEA
                     YGDRAVPDLRDWMADFRADLHVRRSVPGVLASIGTSRAAEALVRQLRRRDPDVAEAVI
                     EALFKFRDRRAGHRFRERDIKPEILHFVREACRAAAAGTSGKDGGEIRASPA"
     gene            complement(2227..2574)
                     /locus_tag="PKV64_04075"
     CDS             complement(2227..2574)
                     /locus_tag="PKV64_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPN16293.1"
                     /translation="MVITKREKGGVVILDLEGEIRRSDTAGVSVHQHVKEQLEAGKRD
                     LLLNFGNVPFIDSFGIGEILASYISTQNSGGRLKLTGISKRLFLVFQVTQLTRVLDIL
                     DSEEKALESFLKS"
     gene            2855..3757
                     /locus_tag="PKV64_04080"
     CDS             2855..3757
                     /locus_tag="PKV64_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPN16294.1"
                     /translation="MAIGDLHGAYAPFVRILTDLNIIDDRLHWAAGKTHVVQMGDVMD
                     RGPDARRILDLIRRLEDEAREAGGAFHMLLGNHEEMNILGLSFDIKGYVTAEQFRSFL
                     PVWSRYRKQTEALTGPENIPAWERFIEGNFQARNTYSRYFNDNYGRWLARRPIVLKIN
                     DIVFVHGGLTEPLSALSCELINAETAREFERYFAREKFEWVWMYRTDGPLWYRDLAIK
                     DEETIREETDRILANLGARAIVIGHSPTTAALSGSVSRLGGKIWVIDTGLWINDGGHE
                     AVLVITGDRMEMVPVLRPAGEVRQ"
     gene            3754..4572
                     /locus_tag="PKV64_04085"
     CDS             3754..4572
                     /locus_tag="PKV64_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16295.1"
                     /translation="MSTRGRSLLLFIGGAVLWLFVAAAVSQEEPSMNKIEAIESYLAS
                     APVVVVEKDVEPGRTMPWRLTLDDGRIRGRALFKYIDRRTALPTRHSYRYELAAYALS
                     RLLELEIIPPVVEREIEGTRGSLQWYLENCRSLRDRERLREEPPVRTDFLDELADVQV
                     FEFLAADECGDKDDTLVHVDSWKICRVDFSEAFRPGTELPASCVFHRCSRSLFHRLES
                     LTLAALTSRLGAFLQPDEIAALFIRSRRVAGIIRGLIREQGEAAVLFERKSPKS"
     gene            4582..5952
                     /locus_tag="PKV64_04090"
     CDS             4582..5952
                     /locus_tag="PKV64_04090"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HPN16296.1"
                     /translation="MKDAEDPSGTAAPAAAQAAARVLRILTFVRPEELTKALLLTLNS
                     FLIIFGYYQIKAVREGLLLAAHSASVKSYLAIPQAVLLVFVIWGFAKLSSKVPRHLLI
                     TWVTLFCISNLLIFDLLNLVGVPVSILGIAFFIWIGMYNQMIPAQFWGFANDIYDEEQ
                     GKRIFPLIALGASLGGVSGPLVARKLIPVFGSYGMILTTAGILGFTILLSWAIHRLDL
                     REDREGTGRPAESKASPLLPSGGFRLIFKSRYLLYIALMIGLYNFINALGEFMFSDLQ
                     QKMALRELGTAATGGIALQNAIGTAFAGYQSLGNTIGLGIQLVLVSRIFRWVGVAGAL
                     LFLPVIALGGYGYAAFGASLVLLKWIKAVENGTDYSLMKTTRSALFLVTRREEKYKAQ
                     AAIETFFVRAGDALCAGVVWAGTAALSFSIEGFAAINAAGVSLWILLCVLIAREHRRK
                     KAAVRE"
     gene            complement(5958..6386)
                     /locus_tag="PKV64_04095"
     CDS             complement(5958..6386)
                     /locus_tag="PKV64_04095"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN16297.1"
                     /translation="MSDEAASANLSVSAEMSEIDRLRDFLKDRLRGVRISGDDLFRLD
                     LALFEILVNIVRYGYPDGAGEIRLDIRVQPDEIRLEIRDHGIPFDPQAVPPPAMDEIL
                     SGRKKGGLGIHLVRNLTDGMMYRREGPENVLVLRKSISGE"
     gene            complement(6580..7509)
                     /locus_tag="PKV64_04100"
     CDS             complement(6580..7509)
                     /locus_tag="PKV64_04100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPN16298.1"
                     /translation="MTSLMALRRGGLGDLLVVLPAVRLLRMTLPEARITLVARPIFGR
                     LFLDSGIVDAVADADDFRWAALAGADGLSERFKPDPAEIDLAAGWFHSPSAAVFERSA
                     AGLWPGAGILTVSADPGTGRPLSVSFFEGTAAGLRNLGLPSAPFESCAFLPRPRSPER
                     ISAPRRPYAVVHPGAGSRLKRWPGGRFRQAVGSLAASGLSGAVVLGEAESFLKVQWTA
                     GVLPPGWLLLDRPAVPELAALLSEASLYLGNDSGVTHLAAVLGVPGAAVFREEFAGAW
                     RPGGKITQVSAADVRDIPVEAVLSSLDLPAGRT"
     gene            complement(7684..8376)
                     /gene="phoU"
                     /locus_tag="PKV64_04105"
     CDS             complement(7684..8376)
                     /gene="phoU"
                     /locus_tag="PKV64_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPN16299.1"
                     /translation="MERVFDEELQFLKEKILRMAALVEDALSLAIDALKEQKEEPARA
                     VLKNEENINVLDVEVDEICMRLLALRQPMAGDLRFITAGMKIGSDLERMGDLAVNVAE
                     LALDLARLPLLKPLIDIPRMSLLAQAMVRDAIEAFINRDDALGRDVCLRDDEVDALDD
                     QIFRELLTFMMEDPHNIRRAVALILVSRNLERIADHATNIAEDVIYMVRGKTIKHHIE
                     KNGIPRSTENQA"
     gene            8585..9484
                     /locus_tag="PKV64_04110"
     CDS             8585..9484
                     /locus_tag="PKV64_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPN16300.1"
                     /translation="MERKEAQVPVWKTLAAFLAAGALVLSAVFCGGKKTGSGGAAALQ
                     IKGSDTMVNLGQAWAEAFNTLHPEINVAVTGGGSGTGIAAILNGTCDIAESSRDLEEK
                     EIQQAETAGMVVHETIVALDGIVVVVHPSNPVSALTMDQLREIFMGNVKRWSAFGGEN
                     RPIVLLSREVNSGTHIFFKEHVLRRGKSKGPEEFAPAALMMPSSYAIAEETARNENAI
                     GYYGLGYISPRQKVLSVSRDDRSPAVSPTIETVRSGAYPISRPLYFFTRGGPSGTVKD
                     ILDFVLSPEGQEIVRATDFVPVR"
     gene            9481..10362
                     /gene="pstC"
                     /locus_tag="PKV64_04115"
     CDS             9481..10362
                     /gene="pstC"
                     /locus_tag="PKV64_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPN16301.1"
                     /translation="MKLSPRRLREFVIERLIFLSGIASIVFVLLIFVFLLREGVSVLK
                     TASPGEFLGGHLWYPISAPPRLGILPLILGSLLVTAGAVVIAVPLGIAAAFYIAEVAP
                     KRLRAILKSFVEVLSAIPSVVLGFIGITVLVPFLKSLLNLPTGLTAFSGSLMLAFMAL
                     PTIITISEDAINAVPREYRDGALALGATHWQTLHRVIIHGALPGIIAAAMLGIGRVFG
                     ETMAVMMVTGNAAVIPHSLFEPVRTLTATIAAEMGEAAKGSPHYFALFAVGIVLFIIS
                     FAINLTADLLLNRGRKK"
     gene            10359..11201
                     /gene="pstA"
                     /locus_tag="PKV64_04120"
     CDS             10359..11201
                     /gene="pstA"
                     /locus_tag="PKV64_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPN16302.1"
                     /translation="MRRELRGQKIGFGVLLLATLLVVIPVAAIVALVIAKGWGALSWE
                     FLFSMPRDGMRAGGIFPAIIGTVYLVFISLAFALPIGVMAAIYLTEYAKDNLLTRIIN
                     LAIINLAGVSSVVFGLFGLSLFVVFLKFGVSIISGALTLGIMALPVIITTTREALRSV
                     PDAYREVSFSLGASRWQTVRHAVLPRALSGILTGAILSVGRVAGETAPILFTVAAFYL
                     PNLPTGLSDQAMALPYHLYVITTQIPGIPESFRYGTALVLIGIVLIMNVIATVVRSRA
                     SRKK"
     gene            11198..11965
                     /locus_tag="PKV64_04125"
     CDS             11198..11965
                     /locus_tag="PKV64_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008396237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HPN16303.1"
                     /translation="MSAPAGTVKVSVRGLSVSYGAAAALKGLDLDIASGEILSIIGPS
                     NSGKTSFLRSLNRMNELNPRARTAGTVLLDGADVVRDMSAEAVRKRIGMIFAMPVPLP
                     LSIRDNITYGPRMAGVRDAAMLDGLIEESLRAAYLWEEVKDRLNMPGQKLSGGQQQRL
                     CIARTLALKPDVILYDEPCSGLDPISTAKVEESMLSLRKSHTLVLVTNNTKQAARVGT
                     RTAFFLMGEMIEVGPTSRLFTAPADKRTEDYIIGRFG"
     gene            11970..12752
                     /gene="pstB"
                     /locus_tag="PKV64_04130"
     CDS             11970..12752
                     /gene="pstB"
                     /locus_tag="PKV64_04130"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPN16304.1"
                     /translation="MKKAIDPRPLPPVLEVRGLNLWYGDFQALSNVSVSFKPRAITAL
                     IGSSGCGKSTLLRSLNRMNDLVEGARIEGEILFNGKDIRRKDCSLTVLRKKVGMVFQR
                     PNPFPLSVFENIAFGPRVHRLAAGGDLDRRVEESLRAVNLWDELKDRLKKNALSLSLE
                     QKQRLCIARLVAVEAEVLLMDEPCSTLDPMATAKVEELMRRLKERYTIVIVTHNMQQA
                     ARVSDETGFMLLGELVEFGRTAEIFTRPSDKRTEDYITGRFG"
     gene            complement(12771..13130)
                     /locus_tag="PKV64_04135"
     CDS             complement(12771..13130)
                     /locus_tag="PKV64_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPN16305.1"
                     /translation="MPGIYPEMIMGLPEIETPLAGIRGRLIQGELTQAVFFDIAAGSK
                     VPPHAHCGQWGLMIEGEMTLTIGGETKTYRKGDRYFIPGGAVHSAAFLTRVFVLDVFD
                     DPARYRAKSAPADAGKE"
     gene            complement(13172..14002)
                     /locus_tag="PKV64_04140"
     CDS             complement(13172..14002)
                     /locus_tag="PKV64_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16306.1"
                     /translation="MNLKIEHHRRFGLAAAFLLGTAVVLGVGAAGADILSTLRLSEAA
                     AKEGILEGLLTGSPYDQKAFAAFKALPPSARAEAVRGGLAWIKTYSESPAFKAAYADF
                     RSEQKPGPPDKVIPADESLKQQKANFEKQVAEMQENLAGLDAETRKAVEEGIKQMRAR
                     MEEMEKDPGQMALMRQGKEMESGENAEQYAEQLKSWEEEYPADVRGLIKRRIREFLDT
                     SAGVDFSAKLVKRKSRMYFADEAYEDKPSDWKICFRAGKEATQAAREFAAAWLAELEK
                     "
     gene            14138..15688
                     /locus_tag="PKV64_04145"
     CDS             14138..15688
                     /locus_tag="PKV64_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16307.1"
                     /translation="MHGKRIFFALAAVALILGTAAALAAPQSQRPPEYREIMAAQNIS
                     DLAARLKELERIKAAYPQSPLRPMLEEAILVTKVKMCAALDEVLALQKTVVGQGEGFD
                     RIYSYFAAAMDILHHPNLARFDAAGVVQAVVSYRNGADRLLKEEAFIRSVPEEERPYL
                     FEYGNNINVVAALACVNAGEPSRALDFLKVYRSAGGAEDDAYSYASAEASVKLGKNED
                     ALGGYLAAAVENYRDAEAKARDLWATVKGTPEGFEAALEIRQRALPFHPEAFAVPADW
                     KGKTVLAEIFTGSECPPCAGADLGFDGLIESVPETHLAILEYHLPIPRPDPMINPATR
                     ARQQYYGVNSTPSTFFDGARKASGGGGRAGAEGKYKEYLAEIKSLLKEEPAVSFTVKA
                     VRDGDEVAVDVNAEKNAAGAETFAALVEKEVRFKGGNGIVFHKMVVRDFQAVSPENPR
                     VVFDLAASEQTTDAYLTEFERTYDRIPNYKFADRRNRIGREGLRVIVFAQDKQTKKVL
                     NAAAADVK"
     gene            15710..18295
                     /locus_tag="PKV64_04150"
     CDS             15710..18295
                     /locus_tag="PKV64_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16308.1"
                     /translation="MPPEYAPKTIRFMEAAYAAQGVLRPLRRQRYEPGTVLEYELRGV
                     WPAARARVSLEILKFAGGGFAGQVYQVAARSIVPIEDQVEGLEAGRTYALKILIPPSR
                     FAARVRNFFYGFGFQAPFSLQSLAAAGRSQALWQKFIRRAARVEFGRDDAVADVIGTF
                     RDERLGSYGELGEWVDGRMWRFEIDDDLDSRRRWKPGDPEDGIGSPEYRTKREFMKRL
                     TAMMRRMGAEELARQYEWGSLKSQPNAMKRIASDPDPRAGLTAVDFRAGMALTPLNPQ
                     CPVDFKLILRGLGRGRLVQFDKGDLRKLERFAGEHAGDFADLRPALEELKREDAAYRD
                     SLVDWTHHHVRLFSGRLRRRIMAGFRESWRVRNITDDRTASVLAKSGVLSLLFLGAPG
                     LALASPLLFVFAWPGRIWWKYPLWLLPLILTPLVRKIWGRDDFRRHCGRMLTSPRYIL
                     RAGRARIAEAMIRWVRTGRASEERARNISSAPWRYYAQLPFSLLPPGFHRFLTEKAYF
                     KERIRALFIQPARLLVRPAERERWMIGMIDQGGKNGLLTAPEVERIKAQVKEPYIQKY
                     LKSLAVHAATLFVSETVFLVAAVVYVLAHPELTWQAATLRAGLIIGALNLLPVSPGSL
                     VRGFYVVGLMIKERNFKDYNIALGVSFLKVFGYLAFPIQMTYRYPDLARFMAGHWANE
                     AVHRVPVFGEKGAWLEHFVFDAFYNFPLTLRRRIRTRAERRAGEPKKSWTTPLLALAA
                     AVVLGGLGAGFAAVAGGAPSFNRTWGLAVWVPVLAGAAIARAASGFQIGARVLRGMTG
                     GALLGAFAGLASHIAALAPFFGAAAAAENVPPVLAAAKSGVTLMLLFGVLGLGGALFA
                     ETRRP"
     gene            complement(18306..20633)
                     /locus_tag="PKV64_04155"
     CDS             complement(18306..20633)
                     /locus_tag="PKV64_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN16309.1"
                     /translation="MKRIRSRIVVFLAVSFFSAFGPPAASSEESAGVLSGFVLDYESR
                     RGLADVRVILPAGEGPGRTERSAVTGKGGAFEFRGLPAGVYSCLFDRSGYAPRAVEGL
                     RVDPAVPVRLDVEMVRLPGPLREEVTVTAEAGPSRIERTAGAVSVEGERLRQTPGSVD
                     DVSRVLKATAGISQVNDTSNELIVRGGSPWENAFYVDQIPFYDINHFQVQGGSGGLIG
                     VINASWVRSLNFFKGGFHAAYGDRMSSVIEMVFREGDRERVQAQVDINTAGFGGGVEG
                     PILGGRGSFLVSAKRSYHDLVTKIIGYGVAPRFGDVHFKAVLDLGPRHRLELLDVNAD
                     SRLSYDAEEAVAMGFTRALNYKNRQNTAGLCWYAGWSDSYSTVTTLSWSRYRNADELL
                     DSVTDSAAFLFDETTDIIGLRHVGGLVLSGRLRASLGFEAKAEIYDFNNYYGAYVDPY
                     ASLIPPVLARGDLTVAKGAVFAGLDWRPAPPLTVSLGARLDVLSHNRTAEVSPRLSAA
                     WAVSPRLTLKAAAGIFRQAVPTAILGITPAVRDNRNPAAAHFLFGVSHDVSKDFRWTL
                     DVYDKQYRRLPVTPEDPEFSVFDLCLDYGVYRSFTTVTDDGQAAARGLEITAEKKPGG
                     TWHLFAGASLFRSRFRDALGRWRDRLNDNRYVLTMIGGVRIARKWNLGLRWDLAGGVP
                     YTPYDIARSTALNAAVLDVTRLDSTRYPDYSSVSLRADREFRFRTSVLLAYLSVVNLL
                     DRENIARYYWNRVENRQDIVPQAPILPVFGLEYRF"
     gene            complement(21077..22237)
                     /locus_tag="PKV64_04160"
     CDS             complement(21077..22237)
                     /locus_tag="PKV64_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPN16310.1"
                     /translation="MTKEDARRFMSRREFLAGGLAAFAGLPMLGKTGFAARPGTAPAG
                     LDEKIGQMIMVGFRGLTLEADNPVIADIRDRKIGGVVLFDYDVPSQNPVRNIASPEQV
                     GALIASLQETAATPLFIAVDQEGGRISRLKEKYGFPATVSQKHLGGLDDAAVTRACAE
                     AEAAVLAEAGFNLNFAPVVDLDVNPENPVIGGLERSFSADPAVVVRHAREVIQAYHRA
                     GVLTTLKHFPGHGSSKNDSHLGFTDVTGTWSKRELEPYAEIIGEGLCDAVMTAHVFNA
                     EIDPRYPATLSRPAIQGLLREGMGYDGLAVSDDMQMKAVSANFGLKEAVIRAVDAGLD
                     ILIFANNSVFDPEIAAAAAGILKGAAAAGVIPEARFDRSYERIMRLKRRMRR"
     gene            complement(22287..24044)
                     /gene="aspS"
                     /locus_tag="PKV64_04165"
     CDS             complement(22287..24044)
                     /gene="aspS"
                     /locus_tag="PKV64_04165"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPN16311.1"
                     /translation="MSEETEHASWKRTHYAGALRPAHEGSEVVLCGWVQKTRDMGHLI
                     FVDIRDREGLAQAVFQTDRPELMEEAKRLRNESVVGVRGRVRRRSAVNPDLPTGEVEV
                     EASALRVFAPSKLPPFAIVDPPQASEELRFKYRYLDLRRPKMQRQIRLRHDAALAVRN
                     YLNRNGFLEIETPILANPTPEGARDYLVPSRISKGRFYALPQSPQQFKQTLMIAGFDR
                     YFQIVRCFRDEDLRADRQPEFTQIDIEMSFPEREEFFELNEGLMEAIFALSGRTIGRP
                     FARLTYAEAMEKYGSDKPDLRVPLEMRDLTAAARDIASDVIRGALAAGAELKGLLVPG
                     AASMSRGQLDKLGEKAKALGAKGLFWIKKQNGWKSSLKLSEADFDKVWASLGGAENDL
                     ALFIADARLAALKALGEIRRTWPGDESARKEMLRFVWVTDFPLFEWSEEEQRHVSMHH
                     PFTAPAAGDLPLLETDPARVRAQAYDLVLNGVEVGGGSVRIHDRDLQRRIFKVLGLSE
                     AETEEKFGYFLEALSFGAPPHGGIAFGFDRLVMLLAGEESIREVIPFPKTTSALDLMT
                     GSPSAVDERQLSDLGLKIK"
     gene            complement(24162..26465)
                     /locus_tag="PKV64_04170"
     CDS             complement(24162..26465)
                     /locus_tag="PKV64_04170"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPN16312.1"
                     /translation="MFKRSAIVLALSVFLSVFPASPQNKKIEFDHQAAFGYLKDLAAD
                     TMLGRKSGEPSGLRAAEYIAAKLKSWGLEPAGPNNGYFQDFTFESYDVQRGASLGIVA
                     HNRTRDFVYGEDWIQSRYSGSGLFGADVVFAGYGISAPDKDFDEYAAVDVKDKLVLFS
                     TDTPVRLRDALREEAKLENRIKAARSRGARGVLLFRSDTQPLGNYFRGGLDKEIYDPG
                     FVILSVERKIVDFIFKWQRGDPRHFFRQIEETGKPQSYDLAVQSLINIQVDFNEKAPT
                     QNVLAKISGTDPKLKNEIVVIGAHMDHLGVDMTGDILNGADDNASGTAVVMEAARVMK
                     LNRFKPKRTILFALWGAEEEGLFGSKHYSENPVFPLDQTVANINLDMVGHGTGKVEAS
                     GAYYAPEIYEILKARLPQEIRDNMIFTRGGPGGSDHTYFLYNGVPAFYLDTDGPHFKT
                     NRVGDVIAHIKPDIMRNAGLFVMAALEILSCERDVPILAGRREMFHWRYQTIFNHETP
                     PLEKVIGEHQDSLDQDVDFQLAVVSGNDGLTGDALRMDLLRKLLDGKEAIARSKGLVL
                     FGNEGASERRRPWKTTVVPGLRGLAAIRDDLRWTEALAKQGFAFIVLDDPGVLFGGGG
                     LSEEGKKILEALEKTSLLLIADGLDASQSVALLENIKKPVFLRTGAVPGDAVLDLAKK
                     TGSTIGLVLGKEELAASYAARVQAASRRIGAEYISIVSERCLWGKAGREQMLGVIAGL
                     LGEKLEPAEMTKLFSASFLRVLRPPGR"
     gene            26851..27279
                     /locus_tag="PKV64_04175"
     CDS             26851..27279
                     /locus_tag="PKV64_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16313.1"
                     /translation="MRGFTLVLTGCLFLAGLAVPPVPAADDALPRLVGIWKGIERDMH
                     WEESGFTMNDESPVKAVFEIKEQKGRVFTGVRTWTNRQGTFSEPFSGVIAADTKRLYM
                     VDHEDGYLFADIAGANEIHFYYLESGASAKIIYTKTVRDE"
     gene            complement(27309..28568)
                     /gene="hisS"
                     /locus_tag="PKV64_04180"
     CDS             complement(27309..28568)
                     /gene="hisS"
                     /locus_tag="PKV64_04180"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPN16314.1"
                     /translation="MSADPTPKKALAAVKGTHDILPGEVEVWQKVEAEVAALFGRYGY
                     RELRAPVIEPTELFEKGTGRTTDIVQKEMYTFTDKGGRSITLRPEYTPSVARAIVEHR
                     LDLLGTPMRYYYIGPMFRYDKPQKGRYRQFHQIDAEVFGEKDPAVDAEIIEMADTMLR
                     ILGVTGTETLVNSVGCKTCRPAYARALRTQAEKVRDGLCEDCRRKIETNPLRIFDCKV
                     ASCRELAASFPAIPEFLCSECRDHFAGVRKTLDLSGVSYRFEPALVRGLDYYTKTAFE
                     VVQTGAGQQNAVLGGGRYDDMMKDFGGPDICGIGFACGVERLIALLTLKPEPRRFVYI
                     VHAGDAAKTKAVELARRFRAEGVETLLEFAGRGFKSQFGRANKLGASWVLIIGEDELA
                     RGEFLLKDMATGSQTSGTPDVLIQTLL"
     gene            complement(28565..29521)
                     /locus_tag="PKV64_04185"
     CDS             complement(28565..29521)
                     /locus_tag="PKV64_04185"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HPN16315.1"
                     /translation="MTKPEDFYTLEKPIVALKEQIDALVLAGEPGFNEKAAALRLEIE
                     AEWRKIQPGLIPSQIVQVARHPKRPMTLDYIKALTTDFMEMHGDRRFADDPALVAGLA
                     FYKGRTVAVMGHQKGRTTRERLDRNFGQMNPEGYRKAYRIFREAEKFGFPIITFIDTQ
                     GAYPGIGAEERGQAEAIAYNLKVMSGLRVPIICLIIGEGGSGGALGIGVGNRILMLEN
                     AYYSVISPEGCAAILWKDQSAVRQAAENLHIRASDLLALGVIDKIIPEPPGGAHIDPE
                     TAFKNTDQVLHQTLTKLQAKTADELVEERYAKFRKMGVYVVP"
BASE COUNT         5396 a         9504 c         9293 g         5475 t
ORIGIN      
        1 gcgggcgacg cccggatttc ccctccgtcc ttcccgctcg tcccggccgc ggccgcccgg
       61 caggcttccc ggacgaagtg aaggatctcg ggcttgatgt cgcgctcccg gaaacggtgg
      121 ccggcccggc ggtcccggaa tttgaaaaga gcttcgatga cggcctcggc gacgtccgga
      181 tcccgacggc gcaattggcg aaccagcgcc tcggccgccc gggatgtgcc gatggaggcc
      241 aaaacgccgg gcacggatcg gcggacgtga agatcggccc ggaagtccgc catccaatcc
      301 cgaaggtcgg gcacggctcg atcgccgtaa gcttccaggg tttccgccgc ccggtcgcgg
      361 acggcgggat ggccgagatg ggggacgatc aggggaagcc agtcgcgctt cttcaagcgg
      421 gccgcgctct ccagggcgta atggacgacg ccgggggatt catgcttcag caaccgcggc
      481 agataatcgg tcagaggcgc gccggcgggc atcaggccga gaaccttggc cagctccatc
      541 tggtcgactt cgcgggcggg cgaggcggag gccgcgactt cgccgaacgg ccggcgcatg
      601 acgtcctgat aggaatcaag ggccaggact tcccgaacga tgacatcgag ggcggccggg
      661 tcctcccctt ccgcccattc cggtccgagc atctcgctgt ccaagtccaa gagcgtatcc
      721 aaggcggacg cccggacggc ggccgatttt tccgaaagaa tctgcctcag ctcgggcgtc
      781 attcgttccg ccttcaagag gtcgaacagg ttgtaggcgt agaggacgga gctccgctcc
      841 cggctttcca aaaggtcgac aagcctcttg gccgtgtccg cccgggcttg ttcccggacg
      901 agccgctcgg cgtcgggccg cgaagggacg aggttggccc ggatcgccgg caggtagttg
      961 cggccgatcc ggacgaggat caccagccaa agaccgatga tcccgattgt cacgaagctc
     1021 atccgggccg cgtcgaacgc cagaataacg gcgaagacga cctggagaag gctggccagc
     1081 gcctctccgc atttgctgac aaagatatcg atcgtcatct tggccttgac cttgacctcc
     1141 tcgggcagcg gaagatacag gagctgcttg gtcgcttgaa agaaggtatg ggttaaggct
     1201 ttgtcggacc ccttgaccag gaccgcccag gccgtgatgc cggccgcggg aatgataaaa
     1261 acggcggccg agccaagggc caggatcagg ggcgtcacga acagcgccgc ccggactccc
     1321 aaggcctgga tcgcccgatg ggcgaaaacg gaattcaaga tccacgagac gacgagcacg
     1381 ccgacggaga aagccgccag gaagtcggtt cggcgcgcct cgagcggaaa agcgttcttg
     1441 accgcccaat tgaactgaaa atcgatcatc gtcgaggcgg ccagggcgac gatgatcaag
     1501 cccgaaagaa ggcgaagata ggggctggcc gccagcgtcc ggaaaccatc gctataattg
     1561 acggggcccg ctccgagttc ttcttcgcgt ccgggcgcgg accggagcgc cgcccggatg
     1621 aaagtcatgg cgatcaggta aaagacaagc ccgaggagga tcaggtggcg ggccggcagg
     1681 cggctggata gaagaacgac caggacggag ccggcgatcc cgcccgccaa gccgccgccc
     1741 acgaaaaagc caaccagctg cttggcccgc ctcggttcac agatcgaccc gaccacgacc
     1801 caaaactgag agacggaggc caggacgaag agttcgaacc agaaccagta aagggcaggg
     1861 acaaggcgcc cgccgagacc gaaaagcagc cagaaggcga ccgtcccgac ggcgaaaaaa
     1921 agctggctcc aggcgatata cgcccgccgg tccagcgccc ggattttttt cgtgttccag
     1981 gcggcgaaaa gacccgagag gacggccgag atgaaataca cgaggggaag cgacgtcttc
     2041 gtcaagccca ggcccaagaa aagggacgtc ttggaaacct tggagacgta gatggcggcc
     2101 atcaggaaga aaaaatgggc gcagaggaaa ccggccaggc gctcttctcc cggccggacg
     2161 ggggaaacac ggttgaggac cgggcgcaag cggccgttca tcggcggggc ctcccgggaa
     2221 tccaggtcag cttttcagaa agctctccag ggctttctcc tcgctgtcca ggatgtcgag
     2281 gacgcgcgtc aactgggtca cttgaaaaac caggaagaga cgcttggaga tcccggtcaa
     2341 tttcaagcgg ccgccgctgt tctgggtgga tatataactg gccaagatct cgccgatccc
     2401 gaagctgtcg atgaacggga cgttcccgaa attcaggaga agatcgcgtt ttccggcttc
     2461 cagctgttcc ttgacgtgct gatgcacgga aacgccggcc gtatccgacc ggcggatttc
     2521 cccttccagg tccaagatga cgacgccgcc tttttcgcgt ttggtgatga ccatcggatc
     2581 gcctcgtctt tttcctttac attatcggaa gcgggggatg atgtcaaacg ccgccgccgc
     2641 cttttttctt gactccttat cccctcaggg gcatagtagg gaaagcgggg agggctccat
     2701 cgtgcctcga aaattcattc gccgaggacg ccggagggat cggtttttcg ccgctttcgc
     2761 cttcctttgt ctcctcgttc ttcttccgcc gggccggcct gcgaccgccg ccgttccgcc
     2821 ggtcccctgc gtttttaccg gggtcgggaa aatcgtggcc atcggggacc tgcacggcgc
     2881 ctatgctccg ttcgtccgga tcctgacgga cctgaacatc atcgacgacc gtctccattg
     2941 ggccgccggc aagacgcatg tcgtccagat gggcgatgtc atggaccgcg ggcccgacgc
     3001 ccgaaggatc ctcgacctca tccggcgctt ggaggacgaa gcccgcgaag ccggcggcgc
     3061 cttccacatg ctcctcggca accacgagga gatgaacatc ctcgggctgt ccttcgacat
     3121 caaaggatac gtcaccgccg agcagttccg gtcgtttctc cccgtctgga gccgctaccg
     3181 gaagcagacg gaagcgctga cgggtccgga gaacatcccg gcatgggaac gcttcatcga
     3241 gggaaatttc caagcccgga acacttacag ccggtacttt aacgacaact acggacgctg
     3301 gctggcccgg cgcccgatcg tcctcaagat caacgacatc gtcttcgtcc acggcggcct
     3361 gaccgagcct ctctccgccc tgtcctgcga gctgatcaac gccgaaacgg cccgtgaatt
     3421 cgaacggtat ttcgcccggg agaaattcga gtgggtttgg atgtaccgga cggacgggcc
     3481 gttgtggtac cgcgacctgg ccatcaagga tgaagagacg atccgggagg aaacggaccg
     3541 gatcctggcc aatctcggag cccgggcgat cgtcatcggc cattcgccga cgaccgcggc
     3601 cctctccgga tccgtcagcc gcctcggagg aaaaatctgg gtcatcgata cgggcctctg
     3661 gatcaacgac ggcggacacg aagccgtttt agtcatcacc ggcgatcgga tggagatggt
     3721 ccccgtcctt cggccggccg gagaggtgag acaatgagca cgcgaggaag gagtcttttg
     3781 cttttcatcg ggggcgccgt tctctggctg ttcgtcgccg cggcggtctc ccaagaggag
     3841 ccgtcgatga acaagatcga ggcgatcgaa tcctacttgg cgtcggcgcc cgtcgtcgtc
     3901 gtcgaaaagg acgtcgagcc gggacggacg atgccctggc ggttgacact cgacgatgga
     3961 cggatccggg gacgggccct tttcaagtac atcgaccggc ggacggccct gccgacccgg
     4021 cacagctatc gttacgagct ggcggcctac gccctcagcc gccttctcga actggagatc
     4081 atcccgcccg tggtcgagcg cgaaatcgaa ggaacgcggg gcagccttca atggtacttg
     4141 gaaaactgcc ggagcctgcg ggaccgggaa cggctgaggg aagagccgcc cgtccggacc
     4201 gattttcttg atgagctggc cgacgtccaa gtcttcgaat tcctggccgc cgacgaatgc
     4261 ggcgacaagg acgacaccct ggttcatgtg gacagctgga agatctgccg ggtcgatttt
     4321 tccgaagcgt ttcggcccgg gacggagctt cccgcctcct gcgtttttca ccgctgctcc
     4381 cgctcccttt tccaccgcct cgaatcgctg accctggccg cgctcacctc ccgccttggc
     4441 gcttttcttc agccggacga aatcgcggcc ctcttcatcc ggtcccggcg cgtcgccgga
     4501 atcatccgcg gcctgatccg ggagcagggg gaagcggccg ttcttttcga aaggaaatcc
     4561 ccgaagagct gagctctccc catgaaggac gccgaggatc cgtccgggac ggcggctccg
     4621 gcggccgccc aagccgccgc ccgggtcctc cggatcctga cgttcgtccg cccggaggag
     4681 ctgaccaaag ctctccttct gaccctgaac agttttctca tcattttcgg ctattaccag
     4741 atcaaagccg tccgcgaggg ccttcttctc gccgcccatt cggcctcggt caagagctac
     4801 ctggccatcc cccaagccgt tctcctggtt ttcgtgattt ggggattcgc caagctgtcc
     4861 tccaaagttc cccgtcacct cctgatcacc tgggtgaccc tgttttgcat ctcgaacttg
     4921 ttgatcttcg accttctgaa tctcgttggg gttccggtct cgatcctcgg gatcgctttc
     4981 ttcatctgga tcgggatgta caaccagatg attccggccc aattctgggg tttcgccaac
     5041 gacatctacg atgaggaaca agggaagcgg atctttcccc tgatcgccct cggggcctcg
     5101 ctcggcggag tcagcggccc gctcgtcgcc cgcaagctca tccccgtttt cggatcgtac
     5161 gggatgatcc tgacgacggc cggaattttg ggattcacaa tcctcctctc ctgggccatc
     5221 caccgcctcg atctgcggga ggaccgggag gggaccggcc ggcccgcgga atccaaagcc
     5281 tcgccccttc ttcccagcgg cggattccgc ttgattttca aaagccgcta tcttctgtac
     5341 atcgccctga tgatcggctt gtacaatttc atcaacgccc tcggcgaatt catgttctcc
     5401 gatctgcagc agaaaatggc cctgcgggag ctgggaacgg cggccaccgg cggcatcgcc
     5461 ctccagaacg cgatcggcac ggcgttcgcc gggtaccagt ctctgggcaa caccatcggc
     5521 ctgggaatcc agcttgtcct cgtctcccgc atcttccgct gggtgggcgt ggccggggcg
     5581 ctgctcttcc tcccggtcat cgccctgggg ggatacggct acgccgcctt cggcgcctcg
     5641 ctcgtcctcc tcaagtggat caaggccgtc gagaacggga cggattactc cctgatgaaa
     5701 acgacccgct cggccctgtt tctcgttacc cgccgggaag agaagtacaa agcccaagca
     5761 gccattgaga cgttcttcgt ccgggccggc gacgccctct gcgccggagt cgtctgggcg
     5821 gggacggccg ccctgtcttt ctcgatcgag ggattcgccg cgatcaacgc ggccggagta
     5881 agcctctgga ttcttctttg cgttctgatc gcccgggagc accggcggaa aaaggccgcc
     5941 gttcgggagt agagccctca ctctccggag atggacttgc gaaggaccag gacattctcg
     6001 gggccttcgc gccgatacat cattccatcg gtgaggttgc ggaccaggtg aatccccaac
     6061 ccgccttttt ttctgccgga gaggatttcg tccatggccg gcggcggaac cgcctgggga
     6121 tcgaatggga ttccatgatc ccggatttcc agccggattt cgtccggctg gacccggatg
     6181 tccaaacgga tctcgccggc cccgtcggga tacccgtagc ggacgatgtt gaccaggatt
     6241 tcaaaaaggg ccaggtcgag gcggaacaaa tcgtctccgg atatccggac gccccggagc
     6301 cggtctttga gaaagtcccg aagccggtcg atctcgctca tttcggccga aacgctcaga
     6361 ttcgccgacg cggcttcatc gctcatcctg tcctcgcccg ggccgttcca gcaagccctc
     6421 tcgtttatta tgccataaac ggcatccgcg cgtcagaaga ggctctcgcc ggcgcggaag
     6481 gatggtccat cgcgggaagg aaagccggct gatcagcaaa agggggtgat gatgatgagg
     6541 gaactctccc cagccggcaa aggagaatct gtgtttattt taagtccggc cggccggcaa
     6601 atcaagcgac gaaagcaccg cctcgacggg gatgtcgcgc acgtcggcgg ctgaaacctg
     6661 cgtgattttt ccgccgggcc gccaggcccc ggcgaattct tcccgaaaga ccgccgctcc
     6721 cggaacgccg aggacggccg ccagatgggt cacgccggaa tcgttgccca gatagagcga
     6781 agcttcggaa aggagagcgg cgagctccgg cacggccggc cggtcgagaa gaagccatcc
     6841 cggggggagg acgccggcgg tccattggac cttcaagaac gactcggcct cgcccaggac
     6901 cacggccccg gaaagtcccg aggccgccag cgacccgacg gcctgccgga aacgaccgcc
     6961 cggccagcgc ttgaggcggc ttcccgctcc cgggtgaacg acggcgtaag gccggcgggg
     7021 agccgaaatc cgttccggac tccgcggccg gggaaggaag gcgcaggatt caaaaggcgc
     7081 ggagggaagt cccaaattcc tcagacccgc cgccgtcccc tcaaaaaacg aaacggagag
     7141 cggacggccg gtgcccggat cggcggaaac agtcaaaatc cctgctcccg gccatagtcc
     7201 tgccgcgctc cgctcgaaaa ccgccgccga cggcgaatga aaccagccgg cggccaaatc
     7261 gatttccgcc ggatccggct tgaagcgctc cgagaggccg tccgctccgg ccagagccgc
     7321 ccagcggaaa tcatccgcgt cggcgaccgc gtccacgatc ccggaatcaa gaaacaagcg
     7381 cccgaagatc ggccgggcga caagcgtgat ccgggcttcg ggcagcgtca tcctcagaag
     7441 gcggacggcc ggcaagacga ccaggagatc gcccaagcct ccccggcgga gagccatcaa
     7501 cgatgtcatc ggaaggcgtc tccggggcgg acaggcctct cccgtccgcg gaatcgaagg
     7561 gggagaatgc cggctgtgtt taacagcaga aaagggaaaa tattggtatt aggaaggata
     7621 aaagttttct ccagccggca caagaaaaca ttggttgtat tatagggaaa gaaaaagaaa
     7681 aaatcaagcc tgattttccg tcgatctcgg aatcccgttt ttctcgatgt gatgcttgat
     7741 cgttttgccc cggaccatat agatgacatc ctcggcgatg ttggtggcgt ggtcggcgat
     7801 ccgttccagg ttccggctga cgaggatcaa ggccaccgcc cgcctgatat tgtggggatc
     7861 ctccatcatg aaggtcagga gctcccggaa aatctgatcg tccagggcgt cgacttcgtc
     7921 gtcccggagg cagacgtccc ggcccagggc gtcgtcccgg ttgatgaacg cttcgatcgc
     7981 gtctcggacc atcgcctggg ccaagaggga catccggggg atgtcgatca agggcttgag
     8041 gagggggaga cgggcgagat ccagggccag ttcggcgacg ttgacggcca ggtctcccat
     8101 ccgctccagg tcgcttccga ttttcattcc ggcggtgatg aaccgcagat ccccggccat
     8161 gggctggcgg agggccagaa gccgcatgca gatctcatcc acctcgacgt cgaggacatt
     8221 gatgttttcc tcgtttttca gaacggcccg ggccggctct tccttctgct ccttgagagc
     8281 gtcgatggcc aaggacaggg cgtcctcaac caaggcggcc atccgcagaa tcttctcctt
     8341 gaggaattgc agctcctcgt caaacacgcg ttccataagt ccctccgcgg ccattttcgg
     8401 acattccggg ccgcttggca agagtcagga tgccccgcgt tcatgaagcg gcgatgtgca
     8461 tcccgtgaat ttttcgggaa ggaagacggt ctgaggaggg ggccgggcgg ttttcttgac
     8521 aggatccccc ccccttttta taatgaattt cactatcccg taattatttc caaaggagaa
     8581 gtctgtggag aggaaggagg cgcaggttcc ggtctggaag acgctcgcgg ccttcctggc
     8641 cgcgggcgcg cttgtccttt ccgctgtttt ttgcggcggg aagaaaaccg gctcaggcgg
     8701 agcggccgcc ctccaaatca aaggctcgga caccatggtc aacctcggcc aagcttgggc
     8761 cgaggctttc aacacgctcc atcccgaaat caacgtggcc gtcaccggag gcggctcggg
     8821 gacgggcatc gccgccatcc tgaacggcac ctgcgacatc gccgagtcgt cccgggatct
     8881 cgaggagaag gaaatccagc aggccgaaac ggccggcatg gtcgttcatg aaacgatcgt
     8941 cgccctcgac ggaatcgtcg tggtcgttca tccctcaaat cccgtctccg ccctgacgat
     9001 ggaccagctc cgcgagattt tcatgggtaa cgtcaagcgc tggagcgcgt tcggaggcga
     9061 gaaccggccg atcgtcctcc tctcccggga ggtcaactcc ggaacccaca ttttcttcaa
     9121 ggagcacgtc ctccgccggg gaaaaagcaa gggaccggag gaattcgccc cggcggccct
     9181 gatgatgcct tcgtcttacg ccatcgccga ggaaacggcc agaaacgaaa acgcgatcgg
     9241 atattacggc ctcggctata tcagtccgcg gcaaaaggtc ctctccgtgt cccgggatga
     9301 ccgctccccg gccgtctcgc cgacgatcga gaccgtccga tccggcgcct acccgatctc
     9361 ccggccgctt tactttttca cccggggcgg accgtccggg acggtcaagg acatcctcga
     9421 tttcgtcttg tcgccggaag gccaggaaat cgtccgggcg accgattttg tgcccgtccg
     9481 atgaagctct ccccgcgccg gctccgcgag ttcgtgatcg agcgtctcat ctttctcagc
     9541 gggatcgcct cgatcgtctt cgttcttctg attttcgtct tcctgctccg ggaaggcgtt
     9601 tccgtcctga aaacggcgtc gccgggcgaa tttctcggcg ggcatctttg gtatcccatc
     9661 tccgctccgc cccggctcgg catcctgccc ttgatcctcg gatcgctgct cgtaacagcc
     9721 ggggcggttg tgatcgccgt ccccctcggc atcgccgctg cgttctacat cgccgaggtc
     9781 gctcccaagc gcctccgggc gatactcaag tccttcgtcg aagtcctttc ggccatcccc
     9841 tccgtcgtcc tcggcttcat cggcatcacc gtcctcgttc ctttcctgaa gagcctcctc
     9901 aacctgccga ccgggttaac cgctttctcc ggatccctga tgctcgcttt catggccctg
     9961 ccgacgatca tcaccatctc cgaggacgcg atcaacgccg tcccccgcga gtaccgggac
    10021 ggagccttgg ccctcggggc gacccattgg cagacgctcc accgggtcat catccacggg
    10081 gccctcccgg gcatcatcgc cgcggccatg ctcggcatcg gccgggtctt cggcgagacg
    10141 atggccgtga tgatggtcac cgggaacgcg gccgtcatcc cccattccct cttcgagccc
    10201 gtccggacgc tgaccgcgac gatcgccgcc gagatgggcg aggcggccaa gggcagtcct
    10261 cactacttcg ccctgttcgc cgtcggcatc gtcctgttca tcatctcctt cgccatcaac
    10321 ctgaccgccg atcttctcct caaccggggc cggaagaaat gagacgcgag ctccgcggcc
    10381 agaaaatcgg cttcggcgtc ttgctgttgg ccaccctgct tgtcgtcatc cccgtcgccg
    10441 ccatcgtcgc cctggtcatc gccaagggat ggggagcgct gtcctgggaa ttcttgttct
    10501 cgatgccccg ggacgggatg cgggccgggg ggatctttcc ggcgatcatc ggaaccgtct
    10561 acctcgtgtt catctccctg gccttcgccc tgccgatcgg ggtgatggcc gccatctacc
    10621 tcaccgaata cgccaaggac aatcttctga cccggatcat caacctggcg atcatcaacc
    10681 tggccggagt gtcttcggtt gttttcggcc ttttcggctt gagcctgttc gtcgttttcc
    10741 tgaagttcgg cgtttccatc atctccggag ctctgaccct cgggatcatg gccctgcccg
    10801 tcatcatcac cacgacccgg gaggccctcc ggtcggtccc ggacgcttat cgcgaggtca
    10861 gcttctccct gggcgcctcg cgatggcaga ccgtccggca cgcggttctc cctcgggccc
    10921 tgtccggcat cttgaccggg gcgatcctca gcgtcggccg ggtcgccggg gagacggccc
    10981 ctatcctctt caccgtggcc gccttctacc tgcccaacct cccgacgggc ctgtccgacc
    11041 aggcgatggc tttgccctac catctctacg tcatcaccac ccagatcccg ggcattcccg
    11101 aaagcttccg ctacggcacg gccctcgtcc tgatcgggat tgtcctgatc atgaacgtca
    11161 tcgcgaccgt cgtccggtcg agagcctcga ggaagaaatg agcgcccccg ccgggacggt
    11221 caaagtttcc gttcgcggcc tctcggtctc ctacggagcc gccgccgccc tgaaaggctt
    11281 ggacctggac atcgcctccg gcgaaatcct cagcatcatc ggtccttcca actccggcaa
    11341 gacctccttc ctccgcagct tgaacaggat gaacgaattg aatccccgcg cccggacggc
    11401 cgggaccgtt ctcctggacg gagcggacgt cgtccgggac atgtcggccg aggccgtgcg
    11461 gaagcgcatc gggatgatct tcgccatgcc cgtccccttg ccgctctcca ttcgggataa
    11521 catcacctac ggaccccgga tggccggcgt ccgggacgcc gcgatgctcg acggcctgat
    11581 cgaggaaagc ctccgggccg cttatctttg ggaggaggtc aaggaccggc tgaacatgcc
    11641 cggccagaag ctctccggcg gacagcagca gcggctgtgc atcgcccgga ccctggccct
    11701 caagcccgac gtgatcctct acgacgagcc atgctccggc ttggatccga tctccacggc
    11761 caaggtcgag gagtcgatgc tcagtctccg caaaagccac acgctcgtcc tggtgacgaa
    11821 caacacgaaa caagccgccc gcgtcgggac gcggacggcg tttttcttga tgggtgaaat
    11881 gatcgaggtc ggcccgacca gccgcctctt caccgcgccg gcggacaagc ggaccgagga
    11941 ttatatcatc ggaaggttcg ggtgaggccg tgaaaaaggc gatcgatccc cggcctctcc
    12001 cgcctgtcct cgaggtccgc ggccttaacc tctggtacgg ggatttccaa gccctgtcga
    12061 acgtttccgt ctctttcaag ccgcgggcga tcacggcctt gatcggctcc tccggctgcg
    12121 ggaagtccac ccttctgcgc agcctcaacc ggatgaacga cctggtcgag ggcgcccgga
    12181 tcgaagggga aatcctgttc aacgggaagg acatccgccg gaaggactgc tccctgaccg
    12241 tcctgcggaa aaaggtcggg atggtcttcc agcggcccaa tcccttcccc ttgagcgtct
    12301 tcgagaatat cgccttcggc ccccgggtgc accgcctggc cgccggcggc gatctggatc
    12361 gaagggttga agagagcctc cgggcggtca acctctggga cgagctcaag gaccggctca
    12421 agaaaaacgc cctgtccctc tcgctcgaac agaagcagcg gttgtgcatc gcccgcctcg
    12481 tcgcggtcga agccgaggtc ctcctcatgg acgagccttg ctcgaccctc gacccgatgg
    12541 ccacggcgaa agtcgaggag ctgatgcgcc ggctcaagga gcgctacacg atcgtcatcg
    12601 taacccacaa catgcagcag gccgcccggg tctccgacga gacgggcttc atgcttctgg
    12661 gcgagctggt ggagttcggc cggacggcgg agattttcac ccggccgtcc gacaagcgga
    12721 ccgaggatta catcaccggc cgcttcggtt gaagatgcgc gccccgccga ttattccttc
    12781 ccggcatccg ccggagcgga tttggccctg taccgggccg gatcgtcgaa aacgtcgagg
    12841 acgaacaccc gggtgaggaa ggcggccgag tggacggcgc ctccgggaat gaaataccgg
    12901 tctccttttc gatacgtctt ggtctccccc ccgatggtca gggtcatttc accctcgatc
    12961 atcaagcccc attggccgca gtgggcatgc ggcgggacct tggagccggc ggcgatgtcg
    13021 aagaaaaccg cttgggtcag ctccccctgg atcaggcggc cgcggatccc ggcgagggga
    13081 gtctcgatct ccggcaatcc cataatcatt tccggataga tgcccggcat ccccgcctcc
    13141 tccggatcgc tttcgggcgg cgatccgctt gttatttctc gagctcggcc agccaagcgg
    13201 ccgcgaactc ccgggccgcc tgggtcgctt cctttcccgc ccggaagcag atcttccaat
    13261 cggagggctt gtcttcataa gcttcgtcgg caaaatacat cctcgacttc ctcttgacta
    13321 acttggccga gaaatccacc ccggcgctcg tatcgaggaa ttcccggatt cttctcttga
    13381 tcagaccgcg gacgtccgcc gggtattcct cctcccaaga cttgagctgc tcggcgtatt
    13441 gctcggcgtt ttccccgctc tccatctcct tgccctggcg catcagggcc atctgcccgg
    13501 ggtccttttc catctcctcc atccgggccc gcatttgctt gatgccctcc tccaccgcct
    13561 ttctcgtttc cgcatcgagg ccggccaggt tttcctgcat ctcggcgacc tgcttttcaa
    13621 aattggcttt ctgctgtttc agcgattcat cggccgggat gaccttgtcg ggagggcccg
    13681 gcttctgctc gctccggaaa tcggcgtagg cggctttaaa ggcgggcgac tcggaatatg
    13741 tcttaatcca ggcgagcccc ccgcggacgg cctcggcccg ggcggaggga ggaagggcct
    13801 tgaaggcggc gaaagccttc tgatcgtaag gagagccggt caacaatcct tcgaggattc
    13861 cttccttggc ggccgcttcg ctcaggcgga gcgtggacag gatgtccgct cccgcggccc
    13921 cgaccccgag gacgacggcc gttcccaaca ggaaagccgc ggcaaggccg aagcggcgat
    13981 ggtgttctat ttttaaattc atgcgattcc tccgtcggtg ggatggtttg ggtcgacacc
    14041 ataaagcgcc aaggggggaa agtcaaggac gcgccgccgc ggccggtgga ctccccgcgt
    14101 tttctgatat actgatatcc ttgaaaagga gacccgcatg cacggaaaac gaatcttttt
    14161 cgctctggcc gccgtcgcct tgatcctggg aacggccgcg gccctcgccg ccccgcagtc
    14221 ccagcggccc ccggaatacc gggaaatcat ggccgcccag aatatttcgg accttgccgc
    14281 ccgcttgaaa gagctcgagc gcatcaaggc cgcttatccc cagagcccgc tgagaccgat
    14341 gctcgaggaa gcgatcctcg tcaccaaagt caaaatgtgc gccgccctgg acgaagtcct
    14401 ggcccttcaa aaaacggtcg tggggcaggg cgagggattc gaccggatct actcgtactt
    14461 cgccgcggcc atggatatcc tccaccatcc caacctggcc cggttcgacg cggccggcgt
    14521 cgttcaggcc gttgtttcct accggaacgg agcggaccgg ctcctcaagg aggaggcctt
    14581 tatccgctcg gtgccggagg aggagcgtcc ctatttattc gaatacggca acaacatcaa
    14641 tgtcgtcgcc gccttggcct gcgtcaacgc cggtgaaccg agccgggccc tcgatttctt
    14701 gaaggtttac cggtcggccg gcggcgcgga agacgacgct tattcctatg cgtccgccga
    14761 agcctcggtg aagctcggaa agaacgagga cgccctgggc ggttatttgg ccgcggccgt
    14821 cgaaaactac cgcgacgccg aggcgaaagc ccgggatctg tgggcgacgg tcaagggaac
    14881 accggaggga ttcgaggccg cgttggaaat ccgacaaagg gcattgccct tccatccgga
    14941 agcgttcgcc gttcccgctg attggaaagg caagacggtc ctggccgaga tcttcaccgg
    15001 ttcggaatgc ccgccctgcg ccggggccga cctcgggttc gacggactca tcgaatccgt
    15061 ccccgagacg cacctggcga ttctggaata tcatcttccg attccccgcc ctgacccgat
    15121 gatcaatccg gcgacccggg cccgccagca gtattacggg gtcaacagca cgccgtccac
    15181 gttctttgac ggcgcgcgga aggcgtccgg cggcggcggc cgggccggcg cggagggaaa
    15241 gtacaaggaa tatctggccg agatcaaatc ccttttgaag gaagagccgg cggtctcctt
    15301 tacggtcaag gccgtccgcg acggcgacga agtcgcggtc gatgtgaacg ccgaaaaaaa
    15361 cgcggccggg gccgagacgt tcgccgccct cgtcgaaaag gaagtccggt tcaagggggg
    15421 caacggcatc gttttccata aaatggtcgt ccgggatttc caggcggtct ccccggaaaa
    15481 tccccgggtg gtcttcgacc tggccgcttc cgagcagacg acggacgcct atctcacgga
    15541 atttgagaga acctacgacc ggatccccaa ttacaagttc gccgaccgcc ggaaccgtat
    15601 cggccgggaa gggctccggg tcatcgtttt cgcccaagac aagcaaacga agaaggtgtt
    15661 gaacgctgcg gccgccgacg tgaaataggg ggttcgaaac gggacgttca tgcctcccga
    15721 atacgcgccc aagacgatcc gctttatgga ggcggcgtac gccgcccagg gcgtccttcg
    15781 gcccctccgc cggcagcgct atgagccggg aacggtctta gaatacgaac tccgcggcgt
    15841 ttggccggcc gcccgggccc gcgtttcctt ggaaatcctc aagttcgccg ggggcggctt
    15901 cgccggccag gtctaccagg tcgcggcgcg gtcgatcgtc ccgatcgagg accaagtgga
    15961 agggctggaa gccgggcgga cctacgccct gaaaatcctg attcctccgt cgcgcttcgc
    16021 cgcccgcgtc cggaactttt tctacggctt cggctttcag gccccgtttt cgctccagtc
    16081 gctggccgcg gccggccgct cccaagccct ctggcagaaa ttcatccgcc gggccgcccg
    16141 ggtggagttc ggccgcgacg acgctgtggc cgacgtcatc ggcaccttcc gcgacgagag
    16201 gctcggcagc tacggcgagc tcggcgaatg ggtggacggc cgaatgtggc ggtttgaaat
    16261 cgatgacgac ctcgattccc ggcgccgttg gaagcccggc gatccggagg acgggatcgg
    16321 gtccccggaa taccggacga agcgtgaatt catgaaacgg ctgacggcga tgatgcggcg
    16381 gatgggagcg gaggagctgg cccgccagta cgaatgggga tcgttgaaaa gccagcccaa
    16441 tgccatgaaa aggatcgcct ccgatcccga cccgagggcc gggctgaccg ccgtcgattt
    16501 ccgggcgggg atggccttga cgcccctcaa cccgcagtgc cccgtcgatt tcaagctcat
    16561 tctccggggg ctcgggcggg gccggctcgt ccaattcgac aagggcgatc ttcggaagct
    16621 cgagcgtttc gccggagaac acgccggcga cttcgccgat cttcggccgg ccctggagga
    16681 actgaagcgg gaagacgcgg cctaccgcga ttcccttgtc gattggacgc atcatcatgt
    16741 ccggctgttt tccggccgcc tccgccggcg gatcatggcc ggattccgcg agagctggcg
    16801 ggtccggaac atcaccgacg accggacggc ctccgtcttg gcgaaaagcg gagtcctctc
    16861 ccttcttttc ctcggagcgc cgggactggc cctggcttcg ccgctcttgt ttgttttcgc
    16921 ctggccgggg cggatttggt ggaagtatcc gctctggctt cttccgctca ttctgactcc
    16981 gctcgtccga aaaatctggg gccgggacga ttttcgccgc cattgcggcc ggatgctcac
    17041 gagtccccgc tacatcctgc gggcggggcg ggcccggatc gccgaggcga tgatccgctg
    17101 ggtccggacg ggccgggcct ccgaggagcg cgcccggaac atctcctccg ctccctggcg
    17161 gtattacgct cagctgccgt tttccctctt gccgcccggc ttccaccgtt ttttgaccga
    17221 gaaggcttac ttcaaggaac ggatccgggc cctttttatc cagcccgcgc ggcttctcgt
    17281 ccggccggcc gagcgggagc gctggatgat cgggatgatc gaccagggcg ggaaaaacgg
    17341 ccttctgacg gctcccgagg ttgagcgcat caaggcccag gttaaggagc cgtacatcca
    17401 gaaatatctc aagagcctgg ccgtccatgc cgccaccctg ttcgtctcgg aaaccgtctt
    17461 tctcgtagcg gcagtcgtct acgtcctggc ccatccggaa ttgacctggc aggcggcgac
    17521 gctccgcgcc gggctcatca tcggcgcctt gaacctcctg ccggtttcgc cgggatcctt
    17581 ggtccgggga ttctacgtcg tcggcctgat gatcaaggag cggaacttca aggactacaa
    17641 catcgccctc ggggtcagtt ttctcaaggt cttcggctac ttggcgttcc cgatccagat
    17701 gacataccgg tatcccgacc tggcccggtt catggccggc cattgggcca acgaggccgt
    17761 ccaccgggtt ccggtgttcg gcgagaaagg cgcctggctg gagcatttcg tttttgacgc
    17821 gttctacaat ttcccgctga ccctgcgccg gaggatcagg acgcgggcgg aaaggcgggc
    17881 cggggagccg aaaaaatcct ggacgacgcc gctcctcgcc ctggctgcgg ccgtcgttct
    17941 gggcggcctg ggggccggct tcgcggccgt cgccgggggg gcgccgtcgt tcaaccggac
    18001 ctggggcttg gcggtctggg tgccggtgct ggccggggcg gcgatcgccc gggcggcctc
    18061 cggctttcaa atcggggcgc gcgtccttcg gggaatgacc ggcggcgcgc ttctcggggc
    18121 gttcgccggc ctcgcctccc atatcgcggc cctcgccccg tttttcggcg cggccgccgc
    18181 cgcggaaaac gttcccccgg tcttggccgc ggccaagtcg ggagtcacac tgatgctgct
    18241 gttcggcgtc cttggtctcg gaggcgcgct cttcgccgaa acgcgccgtc cttgaaccgg
    18301 gcggatcaga accggtattc gagcccgaag accgggagga tcggcgcctg cgggacgatg
    18361 tcctgcctgt tttcgacccg gttccagtaa tagcgggcga tgttttcccg gtccaggaga
    18421 ttgacgacgc tgaggtaggc caagagaacg gatgtccgga accggaactc ccggtccgcc
    18481 cgcaggctca ccgagctgta atccgggtaa cgggtcgaat cgagccgggt gacgtcgagg
    18541 acggcggcgt tcagggccgt cgaccgggcg atgtcataag gcgtgtacgg aacgccgccg
    18601 gccagatccc agcgcaggcc gagattccat ttccgggcga tccggacgcc tccgatcatg
    18661 gtcagaacat accggttgtc gttcaaccgg tcgcgccagc ggcccagggc gtcgcggaac
    18721 cgggaccgga acaggctcgc tccggcgaag agatgccacg ttccgccggg cttcttttcc
    18781 gccgtgattt ccaaaccccg ggcggcggct tgaccgtcgt cggtcaccgt cgtgaacgaa
    18841 cggtacacgc cgtaatcaag gcagaggtcg aagacggaaa attccgggtc ctcgggagtc
    18901 accggcagac ggcggtactg cttgtcgtag acatccaggg tccagcggaa gtccttcgag
    18961 acgtcgtggg agacgccgaa aaggaaatgg gcggccgccg gattgcggtt gtcgcggacg
    19021 gccggcgtga ttccaaggat ggccgtcgga accgcttggc ggaagatccc ggccgcggcc
    19081 ttgagggtca ggcgtggact gaccgcccag gcagccgaaa gccgagggga aacctcggcc
    19141 gtccggttgt gggaaaggac gtcgagccgg gctcccaggg atacggtcaa cgggggggcc
    19201 ggccgccagt ccaggccggc gaaaaccgcg cccttggcga ccgtcaagtc cccccgggcc
    19261 aggaccgggg gaatgagcga ggcgtacgga tcgacgtagg cgccgtaata gttgttgaaa
    19321 tcgtaaatct cggccttggc ctcgaatccg agcgaagccc ggagccgccc ggagagaacg
    19381 agccctccga cgtggcggag gccgatgatg tccgtcgttt cgtcgaagag aaaggccgcc
    19441 gaatcggtga ccgagtccaa gagctcgtcg gcgttgcggt agcgcgacca ggagagggtc
    19501 gtcaccgtgg agtaggaatc gctccagccg gcgtaccagc acagcccggc cgtgttctgg
    19561 cggtttttat agttgagcgc ccgggtgaag cccatggcca ccgcttcctc ggcgtcatag
    19621 gacaagcggc tgtcggcgtt cacatcgagc agctcgagcc ggtggcgggg gccgaggtcg
    19681 agcacggcct tgaagtggac gtcgccgaag cgcggggcga cgccgtagcc gatgattttc
    19741 gtgacgaggt cgtgatagct ccgtttcgcc gagactagga acgagccccg gccgccgagg
    19801 atcggcccct ccactccgcc gccgaaaccg gcggtattga tgtccacttg agcctggacg
    19861 cgttcgcggt cgccctctcg gaagaccatc tcgataaccg aggacatccg gtcgccgtag
    19921 gccgcgtgaa acccgccctt gaagaaattg agcgaccgga cccaggaggc gttaatcacg
    19981 ccgatcaggc ctccgctgcc gccctgcacc tggaaatggt tgatgtcgta aaacggaatc
    20041 tgatcgacgt agaaggcgtt ttcccagggg ctgccgccgc ggacgatcag ctcgttgctg
    20101 gtgtcgttga cttgggagat gccggccgtg gccttgagga cgcggctgac gtcgtcgacc
    20161 gaaccgggcg tttggcggag gcgctctcct tcgaccgaca cggcccccgc cgtccgctcg
    20221 atacggctcg gcccggcctc ggccgtcacc gtcacctcct cgcggagcgg gccgggaagg
    20281 cggaccatct ccacgtccag gcggaccgga accgccggat ccacccggag tccctccacg
    20341 gcccgggggg cgtagccgct ccgatcgaag agacaggaat agacgccggc cggaaggccg
    20401 cggaactcga acgctccgcc cttgccggtg accgcggagc gttcagtccg gccgggacct
    20461 tcgcccgccg gaaggatcac tcgaacgtcc gcgagtccgc gccgcgactc gtaatcgagg
    20521 acgaaaccgg aaaggacgcc ggccgattcc tccgaggatg cggccggcgg gccgaaggcg
    20581 gaaaaaaagg agacggccaa aaaaacgacg atccgcgaac gaatcctttt catttccgat
    20641 ccgcgagccg gcctcgcgga aaaattataa tgaaatcccg ccgcctccgc cagggatgtt
    20701 ccgcacgttc cctcgtccgt ttagccccgg cctttcgcgg ccgcgaatca aggggagaag
    20761 cgtgatcagt ctgagccatc ggagccggga cgcccggcat gacgcgcccg atgccgccct
    20821 cctcccgggg aggcggatgc acggaggcga tggccggaca taaagaacgg gaaagacgac
    20881 agggacggcc tccgccgcgg ctttcatgcc gcgttcaacg catcccggga aagaatggcg
    20941 acgtcggaga tcgggacgat catgacgatc aaagtgagaa cggtaaaagg ggcttccgga
    21001 ggcgggcctt cgctccaccg gaccaaacag gccgcgccga aaaagaggat aggggatggg
    21061 acgaggcgat cgaacgtcaa cgacgcattc tccgcttgag gcgcatgatc cgttcatagg
    21121 aacggtcgaa gcgggcttcc gggatcaccc cggcggccgc ggcccccttg aggatgccgg
    21181 cggcggccgc ggcgatctcc ggatcgaaga ccgaattgtt ggcgaagatc aggatgtcga
    21241 ggccggcgtc cacggcccgg atgaccgctt ccttcaagcc gaagttcgcc gaaaccgcct
    21301 tcatttgcat gtcgtcggaa acggcgaggc cgtcgtagcc catcccctct cggagcaagc
    21361 cctggatcgc cggccgggaa agcgtcgccg gatatcgcgg atcgatttcg gcgttgaaaa
    21421 catgggccgt catgaccgcg tcgcaaagcc cttctccgat gatttccgcg tagggctcca
    21481 gctccctttt cgaccaggtg ccggtgacgt ccgtgaaccc caggtgggaa tcgtttttcg
    21541 agctgccgtg gccggggaaa tgcttcaagg tcgtgaggac gccggcgcga tgatacgcct
    21601 ggatgacctc gcgggcatgc ctgacgacga cggccggatc ggccgaaaag ctccgctcga
    21661 ggccgccgat cacgggattt tccggattga cgtcgagatc gacgaccgga gcgaagttga
    21721 ggttgaatcc ggcctcggcc aggacggccg cctccgcctc ggcgcaggcg cgggtgacgg
    21781 ccgcgtcatc caggccgccg agatgttttt gggaaaccgt cgccgggaag ccgtactttt
    21841 ccttgagccg cgagatccgg ccgccttcct ggtcgacggc gatgaaaagc ggcgtcgccg
    21901 ccgtttcctg cagggaagcg atcagggccc cgacctgttc gggggaggcg atgttacgga
    21961 cgggattttg ggacggaaca tcatagtcga aaagaacgac cccgccgatt ttccggtcgc
    22021 ggatgtcggc gatgacggga ttatccgcct cgagggtcag cccgcgaaaa ccgaccatga
    22081 tcatctggcc gatcttctcg tccaagccgg cgggcgcggt cccgggccgg gcggcgaacc
    22141 cggttttgcc gagcatcggc aggccggcga aggccgccag cccgcccgcc agaaactccc
    22201 tccggctcat gaagcgccgc gcatcctctt tggtcattat gcgatgatcc tcggcaaaga
    22261 cgttcggccg gtcctggaaa gaccggtcac tttatcttca atcccaaatc ggagagctgg
    22321 cgctcgtcga cggccgaggg cgagcctgtc atcaggtcca gggcgctggt cgtcttcggg
    22381 aaaggaatga cctcgcggat ggactcctcg cccgccagga gcatcaccag ccgatcgaag
    22441 ccgaaggcga tcccgccgtg gggcggagcg ccgaagctca gggcttcgag gaaataaccg
    22501 aatttctcct cggtctcggc ttcggaaaga cccagaacct tgaagatccg gcgctggagg
    22561 tccctgtcgt ggatccggac ggagcccccg ccgacctcca cgccgttgag gacgaggtcg
    22621 taggcctggg cccggacccg ggccgggtcg gtttcgagaa gcggcaggtc cccggccgcc
    22681 ggcgcggtga acgggtggtg catcgagacg tgccgctgtt cctcctcgct ccattcaaac
    22741 agggggaaat ccgtcaccca gacgaaccgg agcatttcct tccgggccga ttcatcgccc
    22801 ggccaagtcc gccggatctc cccgagggcc ttcagggcgg cgagcctcgc atcggcgata
    22861 aacagggcca ggtcgttttc cgctccgccg agggacgccc agactttatc gaaatcagcc
    22921 tcggacagct tgagggacga tttccagccg ttctgtttct tgatccagaa caggcccttg
    22981 gccccgagcg ccttggcttt ctcacccagc ttgtccagct ggccgcgcga catcgaggcg
    23041 gccccgggga cgagcaggcc tttaagctcc gcgccggcgg ccagggcccc gcggatgaca
    23101 tcggaagcga tgtcgcgggc cgccgccgtc aggtccctca tctcgagcgg cacgcggagg
    23161 tcgggcttgt ccgagccgta tttctccatc gcctcggcat aggtcagccg ggcgaagggg
    23221 cggccgatcg tccgtccgga gagagcgaag atcgcttcca tcaagccctc gttgagctcg
    23281 aaaaactcct cccgctcggg gaagctcatc tcgatgtcga tttgagtgaa ttcgggctgg
    23341 cggtcggccc ggagatcctc gtcccggaag cagcggacga tctggaaata tcggtcgaat
    23401 ccggcgatca tcagggtttg cttgaactgc tggggcgact ggggcagggc atagaaccgg
    23461 cccttggata tgcggctggg gacgaggtaa tcgcgggcgc cctcgggcgt cggattggcc
    23521 aggatcggcg tctcgatctc gagaaagccg ttccggttca aataattgcg gacggccagg
    23581 gcggcatcgt gccggagacg gatctggcgc tgcatcttcg gccggcgcag gtcgagatac
    23641 cggtatttga accgcagctc ctcggaggcc tggggcgggt cgacgatcgc gaagggcggg
    23701 agcttggagg gggcgaagac ccggagagcg gacgcttcga cctcgacctc tcccgtcggc
    23761 aggtccgggt tgacggccga gcggcggcgg acgcggccgc gcaccccgac gaccgattcg
    23821 ttccggagcc gcttggcttc ttccatcagc tccggccggt cggtttggaa aacggcttgg
    23881 gccaggccct cgcggtcgcg gatgtcgacg aagatcaaat gtcccatgtc cctggtcttc
    23941 tgaacccagc cgcagaggac gacctcgctt ccttcgtggg cgggccgcaa ggccccggcg
    24001 taatgggtcc gtttccagga tgcgtgttcc gtttcttcgc tcatcgggat ttcgccttat
    24061 cggaggtatt ccgccgctat tctagaagat tttcaacatg ttgtccaaac cggggaaagc
    24121 ggactccgcc cctccccggc aaaacgaaac caggccggcg cctaccggcc cggcggcctc
    24181 aaaacccgga ggaacgaggc ggaaaacagc ttggtcatct ccgccggctc gagtttttcc
    24241 ccgagcaatc cggcgatgac gccgagcatt tgctcccggc cggctttccc ccagagacac
    24301 ctctcggaaa cgatcgaaat atattccgcc ccgatccgcc ggctcgccgc ctggactctc
    24361 gccgcgtagg aagcggcgag ttcctccttg cccagaacca ggccgatcgt cgatcccgtt
    24421 ttcttggcca ggtcgaggac cgcgtcgccg gggacggcgc cggtccgcaa aaagaccggc
    24481 ttcttgatgt tttccagcaa ggcgacggat tgagaggcat ccaggccgtc ggcgatcagc
    24541 aggagactcg tcttttccag ggcctcgaga atctttttcc cctcctcgct caatcccccg
    24601 ccgccgaaaa gaacgccggg atcgtcgagg acgatgaagg cgaagccctg cttggccaac
    24661 gcttccgtcc agcgaagatc gtcgcggatg gcggccagtc cccgcaggcc ggggactacg
    24721 gtcgttttcc acggccggcg gcgctccgac gccccttcgt tcccgaagag gacgagccct
    24781 ttcgaccggg cgatcgcctc cttgccgtcg agaagtttcc gcagcaaatc catcctcaag
    24841 gcatcgccgg tcaagccgtc gttcccggaa acaacggcca gttggaagtc aacgtcctga
    24901 tccagcgaat cctggtgctc cccgatcact ttctccaggg gcggggtctc gtgattgaag
    24961 atcgtctgat agcgccaatg gaacatttcc cggcgcccgg cgaggatcgg gacgtcccgc
    25021 tcgcaggaca ggatttcgag agcggccatg acgaacaaac cggcgttccg catgatgtcc
    25081 ggcttgatgt gggcgatgac gtcgccgacg cggttcgtct tgaaatgagg gccgtccgtg
    25141 tcgagataga acgccggcac gccattgtaa aggaaatagg tatgatccga gccgcccggg
    25201 ccgccccggg tgaaaatcat gttgtcccgg atctcttggg gaaggcgggc tttcaggatc
    25261 tcatagatct ccggggcgta ataagcgccg gacgcctcga ccttcccggt tccgtggccc
    25321 accatatcca ggttgatgtt ggccacggtt tgatccaggg ggaaaacggg attttcggag
    25381 taatgcttcg agccgaaaag gccctcttcc tccgctcccc aaagagcgaa gaggatcgtc
    25441 cgcttgggct tgaagcggtt gagcttcatc acccgggcgg cttccatgac cacggccgtc
    25501 cccgacgcgt tgtcatcggc gccgttgagg atgtcgccgg tcatgtccac gccgagatga
    25561 tccatgtggg cgccgatgac gacgatttcg ttcttgagct tcggatccgt cccggagatc
    25621 ttcgccagga cgttctgggt cggcgccttt tcattgaaat caacctggat gttgatcagg
    25681 gactggaccg ccaggtcgta cgattgcggc tttccggttt cttcaatctg ccgaaaaaag
    25741 tggcgggggt cgcccctctg ccatttgaaa atgaaatcca cgatcttgcg ttccacggaa
    25801 aggatgacga atccgggatc atagatctct ttgtccaggc cgccccggaa gtagttcccg
    25861 agaggctgag tatcgctccg gaaaagcagc acgccccgcg ccccgcggct tcgggcggcc
    25921 ttgatacggt tctccagctt cgcttcttcc cggagagcgt cgcgcagccg gacgggggta
    25981 tccgtcgaaa agaggacgag cttgtccttg acgtcgacgg ccgcgtattc gtcgaaatcc
    26041 ttgtccggcg cggaaatccc gtatccggcg aaaacgacgt ccgccccgaa caggcccgat
    26101 ccagagtagc gggattggat ccaatcttct ccgtagacga agtcgcgcgt ccggttgtgg
    26161 gcgacgatcc cgagggacgc gccccgttgg acatcataag actcgaacgt aaaatcctgg
    26221 aaatagccgt tgttcggccc ggccggctcg agtccccagg acttgagctt ggcggcgatg
    26281 tattcggccg cgcggagtcc gctcggctcg ccgcttttcc ggccgagcat cgtgtcggcg
    26341 gcgagatcct tcaagtagcc gaaggccgct tgatgatcaa actcgatttt tttattctgg
    26401 ggcgatgccg gaaagacgga aagaaaaacg ctcaaggcca ggacgatcgc ggatcgcttg
    26461 aacatgatca cctctcggtc gattcgttcc gcttaaaata ctccattcat cggcccatgg
    26521 caaaccaacg ccggggatcc gtgatcgtaa ataaccgccg ccccctccaa accgatggac
    26581 gcttccgcct tccgcgagac ggcggaaagg atttcgtttc ccggcttcct gttccgattc
    26641 ttcgctcatc gggatttcgc tccggggaag attttccgcc tccgttcatg aggattttcg
    26701 gcggattgtc caaaaaccgg gcggtccctg aactagttca gtcccttgac gctttcccgc
    26761 cgccgggcct atgataagaa atcaaagagc gcggccgatt cctaataccg gcggacccgc
    26821 tcaagccgca tttccacaag gaggatttca atgcgcggat tcaccctcgt tctgaccgga
    26881 tgcctttttc ttgccggcct cgccgttccc cccgtcccgg cggcggacga cgcgcttcct
    26941 cggcttgtcg ggatctggaa agggatcgaa cgggacatgc actgggagga atcgggcttc
    27001 acaatgaacg atgaatcgcc cgtcaaagcc gtttttgaaa tcaaggaaca gaaaggccgc
    27061 gtcttcacgg gcgtccggac ttggaccaac cgccagggga cgttttccga gcccttctcc
    27121 ggcgtgatcg ccgcggacac gaagcggctg tatatggtcg accatgagga cgggtacctt
    27181 ttcgccgaca tcgccggcgc gaatgaaatc cacttttatt acctggaaag cggcgcctcg
    27241 gcgaaaatca tctataccaa aaccgtgcgc gacgaataat ccccggcggc tgaggcggcc
    27301 gcggaggcct agagcagcgt ttggatcagg acgtcgggcg ttccggatgt ctgcgatccg
    27361 gtggccatgt ccttgagcag gaactcgccc cgggccagct cgtcctcgcc gatgattaaa
    27421 acccaggagg cgccgagctt gttggcccgg ccgaactggc ttttgaatcc gcggccggcg
    27481 aattcgagaa gcgtttcgac gccctcggcc cggaaccggc gggccagctc gaccgccttc
    27541 gttttggccg cgtccccggc gtggacgata taaacgaacc ggcgcggctc gggcttgagg
    27601 gtcagcaggg cgatcaagcg ctcgacgccg caggcgaaac cgatgccgca gatatccggt
    27661 ccgccgaaat ccttcatcat atcgtcgtag cggcctccgc ccaatacggc attctgctgg
    27721 ccggcgcccg tttgaacgac ctcgaaggcg gttttcgtgt agtaatccag gccgcggacg
    27781 agcgcgggct cgaaccggta ggaaacgccg gaaagatcca gagttttccg gacgccggcg
    27841 aaatggtccc ggcattcgga acagagaaat tccgggatgg cgggaaaaga cgcggccaac
    27901 tcccggcagg aggcgacctt gcagtcgaaa attcggaggg gattggtctc gatcttgcgc
    27961 cggcagtcct cgcacaagcc gtcccggact ttttcggcct gggtccggag ggcccgggcg
    28021 tacgcgggcc ggcaggtctt gcagcccacc gaattcacca gcgtttcggt cccggtcacg
    28081 cccaggatcc ggagcatcgt gtcggccatt tcgatgatct cggcgtcgac ggccgggtcc
    28141 ttttccccga aaacctcggc gtcgatctgg tggaactggc ggtagcggcc tttctggggc
    28201 ttgtcatagc ggaacatcgg gccgatgtag tagtagcgca tcggcgtccc cagcaggtcg
    28261 agccggtgct ccacgatcgc ccgggcgacc gacggcgtgt actccggccg gagggtgatc
    28321 gacctcccgc ccttgtccgt gaaggtgtac atctccttct gaacgatgtc ggtcgtccgg
    28381 ccggtgcctt tctcaaagag ctcggtcggc tcgatgaccg gcgcccggag ctcccggtag
    28441 ccgtaccggc cgaaaagcgc cgccacctcg gcctcgactt tttgccagac ctcgacctcg
    28501 ccgggaagga tgtcgtgggt gcccttgacg gccgcgagcg cctttttcgg agtcgggtcc
    28561 gcgctcacgg gacgacgtag acccccatct tgcggaactt ggcgtagcgc tcttcgacga
    28621 gctcgtcggc ggtcttggct tggagcttgg tcagggtttg gtggaggact tgatccgtgt
    28681 ttttgaaggc cgtctccgga tcgatatgcg cgccgcccgg cggctccggg atgattttat
    28741 cgatgacgcc gagcgccagc aagtcggaag cccggatatg caggttttca gcggcttggc
    28801 ggacggccga ttggtccttc cagagaatcg ccgcgcagcc ctcgggcgaa ataaccgaat
    28861 aataggcgtt ttcgagcatg aggatccggt tgccgacgcc gatgcccagc gccccgccgc
    28921 ttcctccctc gccgatgatc aggcagatga tcggcacccg gagcccggac atgaccttca
    28981 ggttgtaggc gatcgcctcg gcctggcccc gctcctcggc cccgatgccg ggataagcgc
    29041 cctgcgtgtc aatgaaagta atgattggaa agccgaattt ttccgcttcc cggaagatgc
    29101 gataggcttt gcggtacccc tcgggattca tctggccgaa gttgcggtcg agccgttcgc
    29161 gggtcgtccg gcccttctga tggcccatga cggctacggt ccgcccctta tagaaggcca
    29221 gtccggcgac cagggccgga tcatcggcga accggcggtc cccgtgcatc tccatgaaat
    29281 cagttgtcag ggctttgatg taatcgaggg tcatcggccg cttgggatgc cgggcgacct
    29341 gaacgatctg actcgggatc aggccgggct ggattttccg ccattcggcc tcgatctcaa
    29401 ggcgcagagc ggccgctttt tcattgaagc cgggttcgcc ggccaggacc agggcgtcga
    29461 tctgttcttt caaggcgacg atcggttttt caagcgtgta gaaatcctcg ggtttcgtca
    29521 ttgagctctc tgcaattgga aatgataccc agatattcta taagccggcc ggaacgcctg
    29581 tcaaggacgt gaatcgcgcc atctaaaatc ctgccgaagg gtgatatatc ttgccattta
    29641 aaaatcttac cgggactgga caaggaaa
//
LOCUS       DAOOXH010000021        28417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_1442_length_28417_cov_7.541252, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000021 DAOOXH010000000
VERSION     DAOOXH010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 28417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28417
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1442_length_28417_cov_7.541252"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2813
                     /locus_tag="PKV64_04190"
     CDS             <1..2813
                     /locus_tag="PKV64_04190"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HPN16316.1"
                     /translation="TYLHVTGDDYVPWERADDDVIDLVADASSYKPGDTARILVKSPY
                     ERAKALVTVERESIMASEIVEIAGSTSEIAVPVTAEMIPNAYVSVLLIQGRTSSASAG
                     AVKDAGKPSFKIGYTGLSVDPAEKKLELDIQTDKAVYKPGETVTLRLQAKNFQGAGTN
                     ADLAVAVVDVGVINLIGYQTPDPFAAFYGERPLSVETAETRVRVVGQRHFGEKGENPG
                     GGGAGSGGGLSLSEVQLRGDFKSTAYWNPSIATDSEGKAEVKFTLPDNLTTFRVMSVA
                     QTRDSRFGRSSADFKVAKSLLLLPALPRFARVGDSFQGGALVTNNSGTKGTVRLSLKA
                     GGLAVSGGLEQVFELEPGASREALFAFKAEAAGRAVLEFRAAMNAHSDGLQAVFPVEQ
                     PRPLVFLGFSGQAETRREEVVRIPPDIHPDLGEIDLRASASALNGLKARLDELVDYPY
                     LCLEQRLSRILPFLIAAPVIRDFKLSPLPPAETEKMIRGTLREVYACQKDNGGFSPWT
                     ESAFDSPYVSAYAVFALLKAYESGVPVDRSRLDAGLDYLRNVLGSKHNPRAYPYNRAS
                     WAAVQAFALYDLALAGRLEASYAEKLFQERGRLPLFGRALLLKAFHRIAPGSEGEKTL
                     TREFLNLAKITAAEARFEEDDPSGLSWIYSSNARTTAIVLQALLETGGSGPVLSGAAR
                     WLVQKRSAPGWPSTQENFFVFYALNEYYRRVEGGSPDFQARLSLAAKTLLEESFRRPE
                     TDFRATVPLTAFPHDRDLPFLAEKTGTGLLYYSLRMSYAPQKPLPPRDEGFAVYKKIE
                     TLDSRPTADIRAGTLAVVTLEVAVPKESLFVVVDDPLPAGFEAVNAHFRTESEEGFRK
                     LAALEGEAGRPWWEGFQHVEMHDNRVLLFADSLRNGIHRYRYLVRALTFGDFGAPGTR
                     VQQMYAPEIFGLSAEQAVKIVK"
     gene            2810..5059
                     /gene="pbpC"
                     /locus_tag="PKV64_04195"
     CDS             2810..5059
                     /gene="pbpC"
                     /locus_tag="PKV64_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HPN16317.1"
                     /translation="MIRRGGRIAALAVLAAVLSLYVPIPKSRLSPLPVLSLNVLDRAG
                     RPLREILSDEGGRCRWIGLSDISPCAAGAAIAAEDRRFFFHRGVDLPAIARAAFQALR
                     RGRVVSGASTITQQVIRNIYRFRRTIPAKIAEAWLAVRLEHTLSKGEILAQYLNRISY
                     GRQAYGIEAASRFYFDKPALDLSPSEAAYLAALPRSPGLFVPERDASLLRARRDAILR
                     KMARNGFLPAGALSRALAEPLRITAVSRPFRAPHFVEYAVSRLSPAERSSFSEIRTTL
                     DLPLQIKIEALLAGHVRTLEKRGLTNGAVVVLENDTRSVRAMAGSIDYFDERRDGQVN
                     GAIALRQPGSALKPLTYALALEKGMTAATLIDDAPTEFGSLNGSFAPENYDARFHGPV
                     RLRSALASSYNVPAVAVLSLLGPDLLFRKLQEMGFRSLQKDPSHYGVGLTLGNGEVTL
                     LELARAYAALARGGLYAPDRTIEAAVRKDGRSGADQAAPVEIRVFSSAASFIVTDILS
                     DRDARVPTFGYGGPLSLPFPAAVKTGTSKDFRDNWTVGYTPRYTVAVWVGNFDGSPMR
                     NVSGVTGAGPLFRDVVLLLEGSEPDVPFAEPPGLIRADICPVSGELPSPDCPAAVTEL
                     FIPGTAPRTKCRFGHQEASGGPGALRVAGRSSEPLQVVFPAEGDVFKMDAVLRPEFQA
                     LTLRARVEAAPLPDTVTWIIDGVPEAVVRRPFSRSWKLRPGSVIIKVRAVLNGRTVES
                     RPVKIQVLS"
     gene            5154..5534
                     /locus_tag="PKV64_04200"
     CDS             5154..5534
                     /locus_tag="PKV64_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HPN16318.1"
                     /translation="MKKEVKTASAPQAIGPYSQGVIAPPFVFCSGQIPLDPATGKMIE
                     GGIEEQTRRALKNVAAVLEAAGSSLAKTVKTTVFLQDMNDFAAMNAVYAGFFPAPPPA
                     RAAVQAARLPRDAKVEIEAIALLD"
     gene            complement(5545..6465)
                     /locus_tag="PKV64_04205"
     CDS             complement(5545..6465)
                     /locus_tag="PKV64_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPN16319.1"
                     /translation="MAKRIFRIAPGFGQGVRLDVFLCRQIRDFTRAQFQRFIDKQQVR
                     VNGTFKKPSYALREGDVVEADVELPPPPPMEPQFIPLDILYADADVAVINKPSGLLVH
                     PGAGRRFGTLVNALLYHFPEVRGIGPEDRLGIVHRLDRDTSGAIVAARSLKAYNDLKR
                     QFKKREVKKIYLALLWGHFAQREGTLDQPIGRHPRHGQKFSIESRKPRIAITDYTVLA
                     RYKDFDLVEVRPHTGRTHQIRVHMAAGGHPVAGDRVYGTGRSRRDIPRLFLHARILGF
                     RHPETGAWLEFEAPLPSDLRTFLETLKKEE"
     gene            complement(6458..7258)
                     /gene="lgt"
                     /locus_tag="PKV64_04210"
     CDS             complement(6458..7258)
                     /gene="lgt"
                     /locus_tag="PKV64_04210"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPN16320.1"
                     /translation="MHPILFKIGPLTIHSYGFFMALGVAAGLGLLYRQARRRGLDANR
                     LIDAAFYIMIVSLVGAKLILFLGDFSYYASHPKELLWIARSGGVFQGGLAFGVLFALW
                     YFRKYKLPTWKIADVVAPALALGHGFGRIGCFLAGCCYGRACRLPWAANFQDAYAHSL
                     TGVPIHQDLHPVQLYEAVLNFGNALVLYLLLRRNKTDGRVFSFYVINYAVIRFFTEMF
                     RGDHSASGYLFEGPSAYLSLSVSQGFCLVALAAGLILLRVFAKRSSRG"
     gene            complement(7239..7730)
                     /gene="lspA"
                     /locus_tag="PKV64_04215"
     CDS             complement(7239..7730)
                     /gene="lspA"
                     /locus_tag="PKV64_04215"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPN16321.1"
                     /translation="MRRYGLHAVLAAAIILLDQLTKAVIVRTVPYYGSLPVIPGFLDL
                     THIRNSGAILGLFGQMKQPWMTVILLVLNAVALGLVIYYFSKTAQRERLVRLALALIA
                     GGALGNIVDRLARGYVIDFVDMHAGRFHWPTYNVADACITIGAVLLIAGVIFGRPHAS
                     DPV"
     gene            complement(7738..10509)
                     /gene="ileS"
                     /locus_tag="PKV64_04220"
     CDS             complement(7738..10509)
                     /gene="ileS"
                     /locus_tag="PKV64_04220"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPN16322.1"
                     /translation="MADEKDYKKTLNLPHTDFAMKAKLTEMEPAMLRKWEDAGLYGKI
                     IASRRGRRTFVLHDGPPYANGHIHLGTAMNKILKDFIVKSKTMKGYLAPYVPGWDCHG
                     LPIEIHVDRLLGEKKKTLSLTAFREECKAYALKFIDIQREDFKRLGVFGEWTEPYLTM
                     NPSYEADILRCLAVFFKNGDVYKGKRPVHWCPSCRTALAEAEIIYKDKTSPSIYVRFP
                     VVSDLGALAPALKGKDVSVIIWTTTPWTLPANLAVAFHPEHEYAVFETGGKAFLTAKR
                     LVPVVAEVLGFRDPKILATIEGRELAGLKARHPFLDRESIFVLADYVTLDDGTGCVHT
                     APGHGYEDYLTGLAAGLEIYTPVDDTGAFTADVPRYAGMNVFQANAVINADMKAGGSL
                     LGEGEISHSYPHCWRCKNPVIFRATAQWFISLDRGGLRSRALDEIKKVRWIPAWGEER
                     IAGMVANRPDWCISRQRSWGVPIPAFVCSACGETIADEKIALHAADLFEAEGSAAWFT
                     RPAETLLPAGTKCPACGGGSFEKENDILDVWFESGSSQRVLGKRADLPWPADVYIEGH
                     DQHRGWFNSSLLIGVETAGASPFRSCITHGFVLDEEGKAMSKSAGNVIEPRDVIGRNG
                     AEILRLWIAMLNFKEDAPFGRAIVQHLVEAYRKVRNTWRFLLGNLQDFDPDRDALPAA
                     ELEELDLWALEQNAAVGRRILRAYDEYEFHSVFHAAYDFFTVDLSAFYLDVLKDRLYC
                     SGKNDRLRRSAQTALFRILRDSLRLLAPLLPFTTDEAWEAMPGFKDKEESIHLAYFPD
                     FSETWLPDARRKTWTRLLALRDQVLKELEKARESKRIGNALEGSVVLQVPETEAGPIE
                     AEGDLLAALCLVSSVRVERTTGKETGVRIEKAGGRKCSRCWNYRISVGGSADHPDLCR
                     RCADVLAGA"
     gene            complement(10638..12215)
                     /gene="nadB"
                     /locus_tag="PKV64_04225"
     CDS             complement(10638..12215)
                     /gene="nadB"
                     /locus_tag="PKV64_04225"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPN16323.1"
                     /translation="MAGDKERLKADVLIIGCGISGSAAALAASKAGLDVLVISKSSDM
                     QESNTRYAQGGIVSLGPDDDPDLLRRDIFESGDGINNPEAVDVLVSDSKPMVDEILIE
                     ELGIDFSRNGEDELDFAREGGHSRRRILHVQDTTGKTIEEAFLAALASRPNVRILADH
                     TAVDLLTVPHHAKNPQAYYREPACVGAYVLDNRSRRVRMILAGRTVLATGGCGAVFLN
                     TSNPKTAVGSGYAMALRAGARIAHMEYIQFHPTVLYHRDCDGFLISETVRGEGARLKS
                     LDGRTFMDKYGGLKDLAPRDEVSRAIYEEMILTNADHVLLDLASYAKVDVKIRFPHIF
                     ETCLRYGIDISREPIPVVPAAHYCCGGVLVDTWGKTSLHGLYAVGEASCTGLHGANRL
                     ASTSLLEGLVWGTRAGRFLAGDRTPPTGFSESDVRRWYYPPQPEEVDPALINQDWLTI
                     RSTMWNYAGLVRTTKRLERAKADLEYLEHRIDKFYREAILDPDIIRLRHGLQVALMIV
                     RAAIANPVSSGAHYRLD"
     gene            complement(12218..13063)
                     /gene="nadC"
                     /locus_tag="PKV64_04230"
     CDS             complement(12218..13063)
                     /gene="nadC"
                     /locus_tag="PKV64_04230"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPN16324.1"
                     /translation="MKTLDVQEAIDRALAEDLPAGDVTTAAIVPPDLKAAAVLLAKED
                     GVLAGIDVFVRVFKTLDPKVAVRSKLRDGSPFRPGQVLGEISGPAAVLLRGERTALNF
                     VQRLSGIATTTQSYVRAVAGTGVRVLDTRKTTPGLRDLEKYAVRRGGGVNHRRDLSSM
                     VLVKDNHLAVAGGVGPAVAAVRAAWGRRFRLEVEAATAAEAMEAAAAGADWVMLDNMT
                     IPEMKKTVRLLRGRVKIEASGNVCLDNIRQIAETGVDFISIGRLTHSARAVDLSLEIK
                     TKRRR"
     gene            13170..14195
                     /locus_tag="PKV64_04235"
     CDS             13170..14195
                     /locus_tag="PKV64_04235"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPN16325.1"
                     /translation="MSKKQTPLIILSVVLSLFLLIVLFRQVSLSEFTETMSALFLPAL
                     LAYMAVSLAASFLRAWRYRILLQPVEISWGDILLVTLVRNAFDDLFPARIGSLSYLYV
                     LNSRLEKPFENAASSFVIAFIYDFLTLGPFVLLALLAVGGGPAGVSAGALIAAALIFS
                     VVVVLVAWKIALFARIARNIALWLMKKTPWIRRPAVVRAFENLDQTVAVLGEKHKPGR
                     RPAVFLLSFLIRLGKYISVFLLLFAVLRSRGVSWEGLSFWKTVLGLTGAEMTAVLPVK
                     GLAGFGTWESAWAAALQWMRFDPRLAILSGLGIHLVTNLFEYALALGSLAVLAVRRKR
                     DRMGRKS"
     gene            14192..15610
                     /locus_tag="PKV64_04240"
     CDS             14192..15610
                     /locus_tag="PKV64_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPN16326.1"
                     /translation="MSIFEDRFHLGPSDFTDVLETALSRGGDFAELFFEYRLHHSILI
                     EEQIIKETAESVGLGLGIRVWSGDQTGFGYTNDLSPERMKKAARSAAAIAVAGGTPSP
                     PAAFVSRSPLAGLYPVAEPAVSADLGRKIALVREAYEAALAVSPAIKKAKAAWGDYVQ
                     HILIANSEGLIVSDVRPMVKLTCTAIAEKGAVREYGFSGGGGRTGLEVFVSVRTPAEI
                     GREAAEEALLLLEAADAPAGEMPVVLAAGEAGVLIHEAVGHLLEADFNRRKTSVFWNK
                     MGARVGTDLVTIFDDPTIPAGRGSYAVDDEGTIPRRTLLIDKGVVSGLLTDRLSAKIL
                     GRPLTGHGRRQDYSEIPIPRMANTYIAAGPHDPEEILRSVGRGFYAKRLTGGQVEDSG
                     KFTFSVSLGYFIENGRLTRPAKQATLIGTSLGILNRLCMAGTDLEFGRPTGTCGKEGQ
                     HVPINDGCPTLKISSMTVGGRG"
     gene            15607..16956
                     /locus_tag="PKV64_04245"
     CDS             15607..16956
                     /locus_tag="PKV64_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPN16327.1"
                     /translation="MTPGAAVDDLKSLAERFVLFGRACGADEIEVGLGDDTEFEVDVR
                     LGRIENLVEAGTRSMGLRVIKDGKTAFAGSSDLTPETIEGLIRNAVRRAELASPDPCA
                     GLPDSFSPAVDPAGLDLYDPAVAVLDPAEKIRLAMETERIALSDSRIVNSHGSSCHTH
                     EGVSVLSNSRGFLGAYRKTFAGLNVGLQAGETDDLAEDFWFSSETHLDALAGPEEIAR
                     TAVARTIRHLKPRKIETQKAPVVFEPLMTGQILGFLFGCLTGTAVYQKSTFLGERLGD
                     RIGREAVTVIDDGLLPRGLGSSPFDSEGVPSRRTVVIENGVLKSFLCNTYAARKIGAS
                     STGNADGSGVGPGNFHLRPGGDAPEEIIRSTAKGLYLVRTLGQGFNPVTGDISLGAFG
                     LWIENGEFAYPVSEITISGNLGDFLMNIERIGSDLIFNGSICGPTIKVSEMQIAGRS"
     gene            16987..17859
                     /locus_tag="PKV64_04250"
     CDS             16987..17859
                     /locus_tag="PKV64_04250"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPN16328.1"
                     /translation="MNKIGQRAGALLGSSILIILGSLIMGLGYALFLIPHHFVPGGVS
                     GAAIIVNFLTRFPVGTLILILNIPIFALGYKIMGRKSAVRSLAGLFISSFFIDFFNVM
                     IGLPSATEDAVLAAIYGGVMLGIGLGLVFRGGASTGGSDIIGQIFNKRFGLSIGMSIM
                     LIDFFIISGSGFVFGGLEAPLYGYIVLFVSSKVIDMVLEGLSFTKMVIITSTKTAEIQ
                     AFILKTLDRSGTALRSRSLYLNREGETIITVISPKQLVELRAFIKSLDPDAFVIVNDT
                     YAVLGRGFKAPHVG"
     gene            complement(17879..18454)
                     /locus_tag="PKV64_04255"
     CDS             complement(17879..18454)
                     /locus_tag="PKV64_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPN16329.1"
                     /translation="MNIRRLIASRRSIRQFRPKPVSRDLLMDFVDAGRLAPSAANLQP
                     LEFLVVDDPAARAGVFPCLKWAAYIQPDGDPKPGREPAAYIIVLVNTKIRDKMFEYDV
                     GAAVENMALAAAGEGVAGCWLISIDRPKIQALFGIPDHVRIDSVLALGYPAETSVVEP
                     YRDSPRYWKDADGVFHVPKRTLDAVARFNTY"
     gene            complement(18451..19923)
                     /locus_tag="PKV64_04260"
     CDS             complement(18451..19923)
                     /locus_tag="PKV64_04260"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HPN16330.1"
                     /translation="MPKDAPSESGRPFLSVFNDVIGPVMRGPSSSHTAGSYHIAATCR
                     MLLGEPPVRAVVSFDPSGSYIETYQTQNADRAFACGLAGRPLTDARFTDVLRTARSEG
                     LDIAFEAGRLGRAGHPNAVRVRLWTASGRRLEILARSTGGGSFAVVRIEGKPVWIDGK
                     KAFRMNFRGPDGRFIRLRAEPVFFPRAAEPLFRSAADLAALARRRGLTAGEAGRLNEI
                     RVLGLSGEDVDAEMLRRFAVMKRSLRSGLDPGQVHLRLLRPAAARILEAEKRNALPSG
                     GRPVRTAARAMAVMQAVNSGAVVCAAPTGGSAGVLAAVAATLAEDGRAGRADILRSLF
                     AAGAIGLVFVRRATFAAEIAGCQVEIGAAGAMAAAAVVEAAGGTARQACDAAAISLQN
                     TMGSVCDLVAGRCEIPCFTRNAAAAASAFLCADLVLGGYENPVPLDDTIDASLASGRI
                     LPSELRVTSRGGLAVTPAARSLERKVRKRGPAGLEKETPP"
     gene            complement(20156..21724)
                     /locus_tag="PKV64_04265"
     CDS             complement(20156..21724)
                     /locus_tag="PKV64_04265"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HPN16331.1"
                     /translation="MPSSRYPFALSSPLSVLLDKRPEDFTRADLIRVIARRGLERITF
                     HYTALDGRLKELRLPVADKAQAEAILAEGERLDGSSIFKGMVETAASDLYVVPLYSSA
                     FLNPFDDRSLDFICRYYTKDGTLAPFAPDSILKRAAELFRKNTGLELYALGEIEFYLL
                     SERGSDLFPTGFQKGYHNSPPFLKSGPIVNEILSRITKINGAVKYAHGEVGCIDGVQS
                     DLPELKGKLAEQLEIEFAPRPIESMADDVVLGRWLIRNIAWRNGCSVTFAPKLEDGAA
                     GTGLHFHMELKRGGRNVMTGPDGALSKDARLLVGGLCRHAESLTAFGNTTSSAYLRLV
                     PHQEAPTYVCWSDLNRSAMIRVPLAWTNKRHLARSLNPRDTALYRAGEGRQTVELRTP
                     DGSALIHLLLAGMTMSAEWALSRKDKATRGESARALAEKLYVKGDIFADKELLSRLAA
                     LPSSCVGSSRILQSERGLYERDGMFPPSVIDYVIRLLQAEDDEFLNKSLDAMPNEERR
                     VRMRSIMHKDIHRH"
     gene            complement(21856..23199)
                     /locus_tag="PKV64_04270"
     CDS             complement(21856..23199)
                     /locus_tag="PKV64_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPN16332.1"
                     /translation="MRKTRAGWKIVPVLILGLVSAAAVSACRPERSGNPSAMEAAASK
                     VRPGLETLLSERLDLIRGRNVGLITNPSGVDSRLSGAIARIRNVPGVRLVALFAPEHG
                     VRGEAQAGEYVPFMIDAASGLPVFSLYGPSQKPQPDMLGGIDAFMRTFDTTGEDKALR
                     PEMTRGIDVLLFDIQDIGTRVYTYAATMAYAMQACAAAGLDFVVLDRPNPVTGAILEG
                     PVLESPEFSSFIGLYPIPLRHGMTIGEMALLINDRFLEKKAKLTVIPVKGWTRGLWFD
                     ETGLPWVTPSPNMPTLDTAVVYPGQVLFEGTNVSEGRGTTRPFEFFGAPWIDAAALTD
                     RLNALGLPGAVFREQWFTPTFSKFQGERCGGCQLHVIDRSVFRPIRTALHIIAVLREM
                     YPDRFAFHDDYFDKVCGTASVRRAFLGGAPAGEIADGFEPGLREFEAVRRPYLLY"
     gene            complement(23242..24345)
                     /locus_tag="PKV64_04275"
     CDS             complement(23242..24345)
                     /locus_tag="PKV64_04275"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HPN16333.1"
                     /translation="MDGERVLSIDAFRGTAVAAMLIAGNPGNAHAVYSNLQPAVWHGW
                     TPADLLFPFFLVCAGVVIPFSLGRRRRAGIAESALIGQILKRSAGLFLLGLFLNFFPA
                     FDCSAVRIPGILQRIALCYSAGALLGLKTKPSARVVLSAVLPAVYAALIFLVPVPGHG
                     AGILEPDGNLPGYLDNLIFGRHLLKPGFDSEGLLSTLPAVAVLLIGTLLGDWLKTPRT
                     MVRKTLGVFSAGLPLTAAGLLLHPVLPINRPLWTPAFAFLGAGLALIVFGLYYYLIEA
                     LQWKRWSWPFRVLGANSITVFAGSVLLEKILRLITVSSGGVRLDAVVFIHERILGPLA
                     APVPGRLIYAVLAALLWIGLTAPLYRHRLSIRI"
     gene            complement(24447..25595)
                     /locus_tag="PKV64_04280"
     CDS             complement(24447..25595)
                     /locus_tag="PKV64_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPN16334.1"
                     /translation="MRGRRLAARAEGLEKTLLRRIADLADSSCVDLGLGELAFPTPRA
                     VLDGVSRGIGRWPLGYSANAGLPELRARIAASAGPDVDPGRVCVTAGSEEALFLALSV
                     LVDPGDEVLVPDPGFPTYARIVRLWGGLPVFYPLAREDGFVLKASRIKPLLSEKTKAV
                     ILNSPANPTGAVHPRTEIERLAGLLTERGILPVSDEVYRELYYGDRRPESIRTWMPDA
                     IVVDSLSKSCAMTGWRLGWCVVPSALAPAVTAIHQMAVMCAPVPSQRAAILVFDGAAD
                     QERKENLAELRRRRNAAVKTLRSRLGLPFIEPEGAFYIFLDVSSWRADLGGSLDIALG
                     LAGRTKVVTIPGIAFGPGGEGFLRLSFAGTPEDFAEGVRRIAAFRSLK"
     gene            complement(25592..26599)
                     /locus_tag="PKV64_04285"
     CDS             complement(25592..26599)
                     /locus_tag="PKV64_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPN16335.1"
                     /translation="MDVIRLARTLIDIPSVTGAEGPAVDFAAAWLEEAGFHVVRQDVT
                     PGRANLFASAGEPAGVVLCSHLDTVPPPLPGAEDETHVRGRGACDAKGSIAAMMTAGR
                     ALLAEGFRRFGILLVVGEETDSDGARAAGKLSDGCRFIVIGEPTQNRLALGHKGVFSL
                     RLRTAGRAAHSAFPHLGKSAVDGLLDVLQDLRSIAWPVDPVLGASTINIGKIGGGTAE
                     NVLASEAWATVMIRCSVPVAVVRDLVFQAAAGRAEIEVVLESSPVRFLQRPGYETTIL
                     PFGSDAPYLEGFGGRMMIGPGEPEDAHTAEEKVEKRALIEAAEIYRRLVRDLDGETAG
                     R"
     gene            complement(26645..27382)
                     /gene="dapD"
                     /locus_tag="PKV64_04290"
     CDS             complement(26645..27382)
                     /gene="dapD"
                     /locus_tag="PKV64_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HPN16336.1"
                     /translation="MTGKPRKSTGGRDARTLAERIASSPKTTPSRVYVQGMFREDDFK
                     NAGFQSFGTGDFRILFGDYETIASWLSRRRKRIRNVHAEITARYSALPMLDLRKVKAR
                     VEPGAIIRDGASIGRDCVIMMGAVINVGAEVGRGTMIDMNAVLGARAIVGRNCHIGAG
                     TVLAGVLEPPGRVPVVVEDRVLIGANVVVLEGVRIGRGSVVGAGAIVVSDVPAGVVVA
                     GAPARIIKNAVDVAPEKITIVGKLRRR"
     gene            complement(27379..28260)
                     /gene="dapA"
                     /locus_tag="PKV64_04295"
     CDS             complement(27379..28260)
                     /gene="dapA"
                     /locus_tag="PKV64_04295"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPN16337.1"
                     /translation="MMIKGVYTLLMTPFTKDFALDEAGLRVLVRRQIEGGVHGLAPLG
                     VTGENPSLNENEIDRMVEITVEEAGGKAKVAPDTCTNNLEQTIARARRFAEIGADYVV
                     VFAPFLVLPNQAGLLDFYRRVADACPVPIVIHNAPERVGVNVEPKTYAKLMDHGNIAG
                     TKDGKKELDHLAKLIHLARGKNFEIFTGKDTTAYPLMAFGGSGMFSVTANILPGVMRE
                     LTDHSLAGRHEEARSLHYAYYDLFAALRLETNPMAVKAALSLLGLPGGPLRPPLSELG
                     DGNREYIRSLLKERGLL"
     gene            complement(28330..28415)
                     /locus_tag="PKV64_04300"
     tRNA            complement(28330..28415)
                     /locus_tag="PKV64_04300"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28379..28381),aa:Leu,seq:caa)
BASE COUNT         5077 a         9152 c         9116 g         5072 t
ORIGIN      
        1 cgacctacct gcatgtcacg ggcgacgact acgttccctg ggagcgggcc gacgacgacg
       61 tgatcgatct cgtggccgac gcgtcttcct acaagccggg agacacggcc cgaatcctgg
      121 tcaagtcccc ctacgagcgg gccaaggccc tggtgaccgt cgagcgcgaa tcgatcatgg
      181 cctccgagat cgtggagatc gccggctcga cctcggaaat cgccgtcccg gtcacggctg
      241 agatgattcc caacgcctac gtctccgtcc tcctcatcca gggccggaca tcctccgcct
      301 cggccggggc ggtcaaggac gcgggcaaac cctcttttaa aatcggctac acaggcctgt
      361 ccgtcgatcc ggccgaaaaa aagctcgagc tggacatcca gacggacaag gccgtttata
      421 aaccaggcga aaccgttact cttcgcctcc aggctaaaaa cttccagggc gcgggaacga
      481 acgccgactt ggccgtggcc gtcgtcgacg tcggcgtcat caacctgatc ggttaccaga
      541 cgcccgaccc cttcgccgcc ttttacggcg agcgcccctt gtcggtggaa acggccgaaa
      601 cccgggtccg ggtcgtcggc cagaggcatt tcggagaaaa aggagagaat cccgggggcg
      661 gcggggccgg atcgggaggc ggtctttccc tctccgaggt ccagctccgg ggagatttca
      721 aatccacggc gtattggaat ccatcgatcg cgaccgactc cgagggcaag gccgaggtga
      781 agttcaccct ccccgacaac ctgaccacct tccgggtgat gagcgtcgcc cagaccaggg
      841 attcgcgttt cggccggagc tcggccgatt tcaaggtggc caaatccctt ctcctccttc
      901 cggcccttcc ccgcttcgcc cgggtcggcg attccttcca ggggggcgcg ttggtcacga
      961 acaacagcgg gacgaaagga accgtccggc tttcccttaa ggccggcggc ctggcggtct
     1021 ccggcggtct tgaacaggtc tttgagctcg agcccggcgc ctcgcgggaa gccctcttcg
     1081 ccttcaaggc ggaggcggcc ggccgggccg tcctcgaatt ccgggcggcg atgaacgccc
     1141 attccgacgg gctccaggcg gtctttcccg tcgaacagcc tcgtcccctg gtcttcctcg
     1201 gcttttccgg ccaggcggaa acccgccggg aggaggtcgt ccgcattccg ccggacatcc
     1261 accccgacct cggggaaatc gacctccggg cttcagcctc ggccctcaac ggcctgaagg
     1321 cgcgcctgga tgagctcgtc gattatccct acctttgcct cgaacagcgc ctgtcccgga
     1381 ttctcccctt cctcatcgct gctccggtca tccgcgattt caagctctcc cctctccccc
     1441 cggccgaaac ggagaaaatg atccgcggaa cgcttcgtga agtctacgcc tgccagaagg
     1501 acaacggcgg cttctccccg tggacggaat cggctttcga ttcgccgtat gtctcggctt
     1561 atgccgtctt cgccctgctc aaggcgtatg agtccggcgt gcctgtcgac cggtcccgcc
     1621 tggacgccgg gctcgattat ctccggaatg tcctcggatc gaagcacaat ccgcgggctt
     1681 atccttataa ccgggcgtcc tgggcggcgg ttcaggcttt cgccctctat gacctggctc
     1741 tcgccgggcg gctcgaggcg tcctacgccg aaaagctttt ccaggagcgc ggccgcctgc
     1801 ctctcttcgg acgggctctc ctcttgaagg cctttcaccg gatcgccccg ggatcggagg
     1861 gggaaaagac tctgacccgc gaattcctca acctggccaa gatcacggcg gccgaagccc
     1921 gcttcgagga ggacgatccg tccggattgt cctggattta ttcctccaac gcccggacga
     1981 cggcgatcgt cctccaagcc ctcctcgaaa ccggcggctc cggccccgtc ctttcggggg
     2041 cggcccgctg gcttgtccag aaacgaagcg ctcccgggtg gccttcgacc caggagaatt
     2101 tcttcgtctt ctacgccttg aacgagtatt accgccgggt ggaaggaggc tcgccggatt
     2161 tccaggcccg cctctccctg gcggccaaaa ccctgttgga ggaaagcttc cggcgtccgg
     2221 agaccgactt ccgggcgacc gtgcccttga cggctttccc ccatgaccgg gatcttccct
     2281 tcctggcgga aaagaccggg acggggctcc tctattacag tctccgaatg tcctacgctc
     2341 ctcaaaaacc tctgcccccg cgggacgaag gctttgccgt ttacaagaaa attgaaacgc
     2401 tggacagccg gccgacggcc gacatccggg ccgggacgct ggccgtggtg acgctcgaag
     2461 tcgcggttcc caaggagagc ctgttcgtcg tcgtcgacga tccgctcccc gccggattcg
     2521 aggccgtcaa cgctcatttc cgaacggaaa gcgaggaggg cttccgcaag ctggccgctc
     2581 tcgaggggga ggccggccgg ccctggtggg aagggttcca gcacgtcgag atgcacgaca
     2641 accgggtgct cttgttcgcc gattccctcc ggaacggcat ccaccgctac cggtatctcg
     2701 tccgggcctt gaccttcggc gatttcggcg ctccggggac gcgcgtccag cagatgtacg
     2761 ctcccgagat cttcggcttg agcgcggagc aggccgtcaa aatcgtcaag tgatccggcg
     2821 cggggggcgg attgcggcct tggcggtcct ggccgccgtt ttgtcgctgt atgtcccgat
     2881 cccgaagagc cggttatccc ccctgcccgt cctttcgctg aacgtcctcg acagggccgg
     2941 ccggccgctt cgggaaatac tctcggacga gggcgggcgc tgccggtgga tcggcctttc
     3001 ggatatttcc ccgtgcgccg ccggagcggc catcgccgcc gaggaccgcc ggtttttctt
     3061 tcatcgcggc gtcgatctcc cggccatcgc ccgggcggct ttccaagccc tgcgccgcgg
     3121 ccgggtggtg tcgggcgcct caaccatcac ccagcaggtg atccgcaaca tctaccgctt
     3181 ccggcggacg atcccggcga agatcgccga ggcctggctg gccgtccgcc tggagcacac
     3241 cttgtccaaa ggtgaaatcc tcgcccaata tctcaaccgc atctcctacg gccggcaggc
     3301 atacggaatc gaggccgcct cccggtttta cttcgacaag ccggccttgg acttgagccc
     3361 gtctgaggcg gcttacctcg ccgccctccc ccgctcgccc ggactgttcg tccccgagag
     3421 ggacgcgtcc cttcttcgag cccggagaga cgccatcctc cggaagatgg cccggaacgg
     3481 atttctcccg gccggcgccc tttcccgggc tctggccgag cccctccgca taacggccgt
     3541 ctcccggccg tttcgggcgc ctcatttcgt ggaatacgcc gtttcgcgcc tttccccggc
     3601 cgagcggagc tcgttttcgg aaatccggac gaccctggac cttcccctcc agattaaaat
     3661 cgaagctctt ctcgccggcc atgtccggac gcttgaaaaa agaggtctga ccaacggggc
     3721 cgtggtcgtt ctggagaacg atacccggtc cgttcgggcg atggccggct ccatcgatta
     3781 tttcgacgag cgtcgcgacg gccaggtcaa cggggcgatc gccctgcgcc agccgggatc
     3841 cgccctcaag cccctgacgt atgccctggc cctggaaaaa ggaatgaccg cggcgacgtt
     3901 gatcgatgac gcgccgaccg agttcggctc gctgaacgga tcgttcgctc cggagaatta
     3961 cgacgcgcgc ttccatggtc cggtccgtct tcgcagcgcc ctggccagct cctacaacgt
     4021 tccggccgtc gccgtcctga gccttctcgg gcccgacctg ctgttccgga agctccagga
     4081 aatgggattc cggagcctgc agaaggatcc gtcccactac ggcgtcggct tgaccctggg
     4141 gaacggcgag gtcacgcttc tggagctggc ccgggcgtat gccgctctgg cccgcggcgg
     4201 tctctatgcc cccgaccgga cgatcgaggc ggctgtgcgg aaagacggcc gatccggggc
     4261 ggaccaggcg gccccggtcg aaatccgggt cttttcgtcc gccgcctcct tcatcgtcac
     4321 cgatattctc tccgaccgcg acgcccgtgt cccgacgttc ggatacggcg gccccttgag
     4381 ccttcccttc ccggccgcgg tcaagaccgg aacatccaag gacttccggg acaactggac
     4441 cgtcggatac actccccgat acacggtggc cgtctgggtg gggaatttcg acggatcgcc
     4501 gatgcggaac gtgtccggcg tcaccggggc cggccccctc ttccgcgatg tcgtgctgct
     4561 ccttgaaggt tccgaaccgg atgttccatt cgccgagccg ccggggctga tccgggccga
     4621 catctgcccg gtttcgggcg aacttccctc gccggactgc ccggcggccg tcaccgagct
     4681 gttcattccc ggcacggcgc ctcggacgaa atgccggttc ggacatcagg aggcgtcggg
     4741 agggccgggc gccctgcggg tcgccgggcg ttcctccgaa cctctccagg ttgtatttcc
     4801 cgccgagggg gatgtcttca aaatggacgc cgtcctgcgg cccgagttcc aagccttgac
     4861 gctgcgggcc cgggtggagg ccgctccgct tcccgacacc gtcacctgga tcatcgacgg
     4921 cgttccggaa gccgtcgtcc gccgtccgtt cagtcggtcc tggaaactca ggccgggatc
     4981 ggttatcatc aaggtccggg cggttctgaa cggccgaacc gtcgagagcc gcccggtgaa
     5041 aatccaggtg ttatcctgag gtttcgatcg tgaagcgacg cgaagcgctg gccgttctcg
     5101 gaacgtttcc gctcctcgcc cggttcccat tatcgtcccc aaggagtgtc cccatgaaga
     5161 aagaagtgaa aaccgcatcg gccccccagg ccatcggacc ctattcccag ggcgtcatcg
     5221 ccccgccgtt cgtcttttgc tccggacaaa tccctctcga tccggccacc ggcaagatga
     5281 tcgagggcgg gatcgaggaa caaacccgcc gggcgctgaa gaatgtcgcc gccgttctcg
     5341 aggcggccgg cagctcgctg gccaagaccg tcaagaccac cgtgttcctc caggacatga
     5401 atgatttcgc ggccatgaac gccgtttacg ccggcttttt ccccgctccg cccccggccc
     5461 gggcggccgt tcaagcggcc cgcctgccgc gggacgccaa ggtggaaatc gaagcgatcg
     5521 ccctcttgga ttgagcccgg ccgcttactc ttcttttttc aaagtttcga gaaaagtccg
     5581 caggtcggac ggcaggggcg cctcgaattc aagccaggcc ccggtctccg gatgccggaa
     5641 gcccagtatc cgggcgtgaa gaaagagccg cgggatatcc cgccgggatc ggcccgttcc
     5701 gtaaacccgg tcgccggcga ccggatggcc tccggccgcc atatggacgc ggatctgatg
     5761 cgtccgcccg gtatggggac ggacctcgac aagatcgaaa tccttgtatc gggccagaac
     5821 ggtatagtcc gtgatcgcga tccggggctt gcggctttcg atggagaact tctgcccgtg
     5881 gcgcggatgg cggccgatcg gctggtcgag cgttccttcc ctttgagcga aatggcccca
     5941 gaggagagcc aggtagattt ttttcacttc ccgctttttg aactgtcgct tgagatcgtt
     6001 atacgccttg aggctccggg cggccacgat cgcccccgac gtgtcgcggt cgaggcgatg
     6061 gacgatcccc aagcggtcct cgggaccgat gccgcggact tccggaaaat ggtacaggag
     6121 ggcgttgacc agggttccga agcggcggcc cgcgcctgga tggacgagca agccgctggg
     6181 cttgttgatg accgccacgt ccgcgtcggc gtagagaata tccaggggga tgaactgggg
     6241 ttccatcggc ggcggcggcg gaagctcgac gtcggcctcg accacgtccc cttcccggag
     6301 ggcgtagctg ggctttttga acgtcccgtt gacccggacc tgctgcttgt cgatgaaccg
     6361 ctggaattgg gcccgggtga aatcccggat ctggcggcaa agaaagacat ccagccggac
     6421 gccttgcccg aagcccggag cgatgcggaa gatcctttta gccacgcgag gagcgtttgg
     6481 cgaacacgcg aaggaggatc agcccggcgg ccagggcgac caggcagaat ccctgggaaa
     6541 cggacaggct caaataagcc gaggggcctt caaagaggta gccgctggcg gagtgatcgc
     6601 cccggaacat ctcggtgaaa aaccggatga cggcatagtt gatcacgtag aatgaaaaga
     6661 cccggccgtc ggtcttgttc cgccggagca gcagatagag aacgagggcg ttgccgaaat
     6721 tcaggaccgc ctcgtagagt tgaacggggt gaaggtcctg gtggatcggc actccggtca
     6781 aggaatgggc ataggcgtcc tggaaattcg ccgcccaggg caggcggcag gcccggccgt
     6841 agcagcaacc ggcgagaaaa cagccgatgc ggccgaaacc gtggcccagg gccagggcgg
     6901 gggcgacgac gtcggcgatt ttccaggtcg gcagcttgta tttccggaaa taccacaggg
     6961 cgaagagaac gccgaaagcc agtcccccct gaaagacgcc gcccgaacgg gcgatccaga
     7021 gaagctcctt cggatgggag gcataataag aaaaatcacc gagaaagagg atgagcttcg
     7081 ctccgaccag ggaaacgatc atgatataga aggcggcgtc gatcagccgg ttggcgtcga
     7141 gaccgcggcg gcgggcttgc cggtaaagca gtcctaagcc cgcggcgact cccagggcca
     7201 tgaagaaacc gtaggaatga atcgtcagcg gaccgatttt aaacaggatc ggatgcatgc
     7261 ggcctcccga aaatgacgcc ggcgatcaag aggacggccc cgatcgtaat gcaggcgtcg
     7321 gcgacgttat aggtcggcca gtgaaaccgg ccggcgtgca tgtcgacgaa atcgatgaca
     7381 tatccccgag cgagccggtc gacgatgttc cccagggctc cgccggcgat cagggccagg
     7441 gccaggcgga cgagccgctc cctttgggcc gttttggaaa aataatagat gaccaggccg
     7501 agggcgaccg cgttgaggac gaggaggatg accgtcatcc agggctgctt catctgaccg
     7561 aaaagaccga gaatggcccc gctgttgcgg atatgggtca ggtcgagaaa tccgggaatg
     7621 acgggaaggg agccgtaata aggaaccgtc ctcacgatga cggccttggt caactgatcc
     7681 aaaagaatga tcgccgcggc caggacggcg tgaagtccgt atcggcgcat cacgtcctca
     7741 cgctccggcc aggacgtccg cgcagcggcg gcacaaatcg ggatgatcgg ccgagccgcc
     7801 gacggagatc cggtagttcc agcaccggct gcatttccgt ccaccggctt tctcgatccg
     7861 gacgccggtc tccttccccg tcgtccgctc gacgcggacc gaagagacga ggcaaagggc
     7921 ggccaggaga tcgccttcgg cctcgatcgg cccggcctcg gtctccggga cctggaggac
     7981 gacgcttccc tcgagggcgt tgccgatcct cttggattcc ctggcttttt ccaattcctt
     8041 gaggacttga tccctcagag ccaggagacg cgtccaggtc ttgcgccggg cgtcgggaag
     8101 ccacgtttcg gaaaaatcgg ggaaatacgc caggtggatg gattcctcct tgtccttgaa
     8161 gcccggcatc gcttcccagg cttcgtctgt ggtgaacggc agaagggggg ccaggaggcg
     8221 gagggaatcc ctcaggatcc ggaacaaggc cgtctgggcc gaacggcgga gacggtcgtt
     8281 tttccccgag cagtagagac ggtccttaag gacgtctaag tagaaagcgc tcaagtcgac
     8341 cgtgaaaaag tcatacgcgg cgtggaaaac cgaatgaaat tcgtattcat cgtacgcccg
     8401 gaggatccgg cggccgacgg ctgcgttttg ttcgagggcc cagaggtcga gctcctccaa
     8461 ttcggcggcc gggagggcgt cgcggtccgg gtcgaaatcc tgcaggtttc cgaggaggaa
     8521 ccgccaggtg ttccggactt tccggtaagc ctcgacgaga tgctggacga tcgcccggcc
     8581 gaagggggcg tcctccttga agttgagcat ggcgatccac agacggagga tctccgctcc
     8641 gttccggccg atcacgtccc gcggctcgat gacgtttccg gccgatttcg acatcgcctt
     8701 tccctcctcg tccaggacaa agccgtgggt gatgcaggaa cggaagggcg acgcgccggc
     8761 ggtttcgacg ccgatcagca gcgagctgtt gaaccacccc cgatgctggt catggccctc
     8821 gatatagaca tcggccggcc agggcaggtc ggcccgcttc cccaggacgc gctggctgga
     8881 gcccgattcg aaccagacat ccaggatgtc gttttctttt tcgaaggagc cgcccccgca
     8941 ggccgggcat ttcgtcccgg cgggaagcag ggtttcggcc ggccgggtga accaggccgc
     9001 cgatccctcg gcctcgaaaa gatcggccgc gtgcagggcg atcttctcgt cggcgatcgt
     9061 ttcgccgcag gccgagcaaa caaacgccgg gatcggcact ccccaggacc gctggcggga
     9121 aatgcaccaa tcgggccggt tggccaccat cccggcgatg cgctcctcgc cccaagccgg
     9181 aatccagcgg accttcttga tctcatccag ggcccggcta cgcaagccgc cgcggtccag
     9241 agagatgaac cattgggccg tggcccggaa gatcaccgga ttcttgcagc gccagcagtg
     9301 cgggtaagaa tgcgagatct ccccttctcc gagaagcgac ccgccggctt tcatatcggc
     9361 gttgatgacc gcgttggctt gaaagacgtt catcccggcg tagcggggga cgtccgcggt
     9421 gaacgcgccc gtgtcgtcga ccggcgtgta gatctccagg ccggcggcca ggccggtcaa
     9481 gtaatcctcg tagccgtggc ccggagccgt gtggacgcag ccggtgccgt cgtcgagggt
     9541 gacgtagtcg gccaggacga agatcgattc ccggtcgagg aacggatggc gggccttgag
     9601 gccggcgagt tcccggccct cgatggtcgc caggatttta ggatcgcgga agccgaggac
     9661 ctcggccacg accggaacga ggcgcttggc cgtcagaaaa gccttgcccc cggtctcgaa
     9721 aacggcgtat tcatgctcgg gatgaaaggc gacggccagg ttggccggca gcgtccaggg
     9781 cgtcgtcgtc cagatgatca ccgaaacgtc cttccccttc agggccggag ccagggctcc
     9841 caggtccgaa acgaccggaa accggacgta gatcgagggc gacgtcttgt ccttgtaaat
     9901 gatttcggct tcggccaggg ccgtccggca ggacgggcac caatggaccg gccgtttccc
     9961 cttgtacacg tccccgttct taaaaaagac ggcgagacaa cggaggatgt ccgcctcgta
    10021 ggacggattc atcgtcaagt agggctcggt ccattccccg aacaccccca ggcgcttgaa
    10081 atcctcccgc tggatgtcga tgaacttcag ggcataggcc ttgcattcct cccggaaggc
    10141 ggtcagggac agggttttct tcttttcgcc gaggaggcgg tcgacgtgga tctcgatcgg
    10201 caggccgtga caatcccacc ccgggacgta gggcgccagg tatcctttca tcgttttcga
    10261 tttgacgatg aaatccttga ggatcttgtt catcgccgtt cccaagtgga tgtggccgtt
    10321 ggcgtacggc ggcccgtcgt gaaggacgaa tgtccggcgg ccccgccggg aggcgatgat
    10381 ctttccgtag aggccggcgt cttcccattt gcggagcatc gccggctcca tttcagtcag
    10441 cttggccttc atggcgaagt ccgtatgggg gaggttcagc gtttttttgt agtctttttc
    10501 atcggccatc gaattctccg gtttttccct aggcgggagt ccgtcctccc cgccgcttac
    10561 cgccggcgtc ttttcgggac gccctatttt atcccctggg ggggaaaagt caattgaaag
    10621 ggagtctgag ccggcgttca gtccaggcgg taatgagcgc cggaggacac cgggttggcg
    10681 attgccgccc ggacgatcat cagggcgacc tgcaagccgt ggcggaggcg gatgatgtcc
    10741 ggatcgagga tggcttcccg gtagaattta tcgatgcggt gttcgagata ttccaggtcg
    10801 gccttggccc tctccaagcg cttggtcgtc cggaccaagc cggcgtagtt ccacatcgtc
    10861 gaacggatcg tcagccagtc ctggttgatc agggccgggt cgacttcctc cggctggggc
    10921 gggtagtacc atcggcggac gtcgctttct gaaaaaccgg tcggcggcgt gcggtccccc
    10981 gctaggaaac ggccggcccg ggtgccccag accagccctt caagaagaga cgtggaagcc
    11041 aggcggttcg ccccatggag gccggtgcaa ctcgcctcgc cgacggcgta gagtccgtgg
    11101 agggaggttt tcccccacgt gtcgacgagc acgccgccgc agcagtaatg ggcggctgga
    11161 acgacgggaa tcggctcccg gctgatgtcg atgccgtagc gcaggcaggt ttcgaaaata
    11221 tgcggaaacc ggatcttcac gtcgaccttg gcgtaggagg ccagatcaag gaggacatgg
    11281 tcggcgttgg tcaggatcat ttcctcgtag atcgcccggg atacttcgtc gcggggggcc
    11341 agatccttca atccgccgta tttatccatg aaggtccggc cgtccagcga tttcagccgg
    11401 gccccttccc cccggaccgt ctccgagatg aggaaaccgt cgcagtcccg gtgataaaga
    11461 accgtgggat ggaactggat gtattccatg tgggcgatcc gggcgccggc ccggagggcc
    11521 atggcgtagc ccgagccgac ggccgttttc ggattggagg tattgagaaa aaccgccccg
    11581 cagccgccgg tggccaggac cgtccggccg gcgaggatca ttcggacccg gcgcgaccgg
    11641 ttgtccagga catacgcccc gacgcaggcc ggctcccggt aataggcctg aggattcttg
    11701 gcgtggtgag ggacggtcag aagatccacg gccgtatggt cggccaggat gcggacgttc
    11761 ggccggctgg ccaaggcggc caggaaggcc tcctcgatcg ttttgccggt cgtgtcctgg
    11821 acgtgaagaa tgcggcggcg ggaatggcct ccttcccggg cgaaatccag ctcgtcctcc
    11881 ccgttgcggg aaaagtcgat tcccagctct tcaatcagga tctcgtcgac catcggcttg
    11941 ctgtccgaga ccaggacatc caccgcctcg ggattgttga tgccgtcccc ggactcgaaa
    12001 atatcccggc ggagcaggtc cggatcatcg tccgggccga gggaaacgat tccaccctgg
    12061 gcgtaccggg tgtttgattc ctgcatgtcg gacgacttgg agatgacgag gacgtccagg
    12121 ccggccttgg acgcggccag agcggccgcc gagcccgaaa tcccgcatcc gatgatcaaa
    12181 acgtcggcct tgagccgttc cttatccccc gccatgtcta tcgtctcctt ttcgtcttga
    12241 tttcaaggct gagatcgacg gcccgggccg agtgggtcag ccggccgatg gaaatgaaat
    12301 ccacgcccgt ctccgcgatc tgcctgatgt tgtccagaca gacattgccc gaggcctcga
    12361 tcttgacccg gccccgaagg agccggaccg tttttttcat ctccgggatc gtcatgttgt
    12421 cgagcatcac ccaatccgcc ccggcggccg cggcttccat cgcctcggcc gccgtggcgg
    12481 cctccacctc gaggcggaag cgccgccccc aggccgcccg aacggcggcc accgccgggc
    12541 cgactccgcc ggcgacggcc aggtgattgt ccttgacgag gaccatgctc gaaagatcgc
    12601 ggcggtgatt gactccgccg ccccggcgga cggcgtattt ttccaggtcg cgcagaccgg
    12661 gcgtcgtctt ccgggtatcg aggacgcgga cccctgttcc agcgacggcc cgaacgtaag
    12721 actgcgtcgt ggtggcgatg ccggacaggc gttggacgaa attgagggct gtccgctcgc
    12781 cccggagaag aacggccgcc ggtcccgaga tttcacccag gacctgtccc gggcggaacg
    12841 gagatccgtc ccgaagcttg ctccggacgg cgaccttcgg atccagtgtc ttgaacaccc
    12901 ggacgaagac gtcgattccg gccaagacgc cgtcttcctt ggccagcaga acggccgccg
    12961 ccttcaggtc cggggggacg atcgccgccg tcgtcacgtc tccggccggc aggtcctcgg
    13021 ccaaggcccg gtcgatcgct tcttgaacgt ccaacgtctt cattttccgg attataggac
    13081 ttcggccggg gccggtcaaa ccgatcccct ctcccgccct ggaggcgaaa agaattgaca
    13141 ggcgcggcgg ccccatggga acataccgcg tgtccaaaaa acagacgccg ctgattatcc
    13201 tctccgtcgt tctctcccta ttcttgttga tcgtcctctt ccgccaagtt tccctgtccg
    13261 aattcaccga aacgatgtcg gctctctttc ttcccgccct cctggcctat atggccgtca
    13321 gcttggcggc gtctttcctg agagcctggc gctaccggat ccttctccag ccggtggaaa
    13381 tctcctgggg ggacatcctg ctcgttaccc tggtccggaa cgccttcgac gacctctttc
    13441 ccgcccgcat cggctccctt tcttatcttt atgtcctgaa cagccgcctg gaaaaaccgt
    13501 tcgagaacgc cgcctcctcg ttcgtcatcg ccttcattta tgattttctg accttaggcc
    13561 cgttcgtcct cctggccctc ctggctgtcg gcggcggccc ggccggcgtt tccgccggag
    13621 cattgatcgc cgccgccttg attttttcag tcgtcgtcgt ccttgtcgcc tggaaaatcg
    13681 ccttattcgc ccggatcgcc cggaatatcg ccctctggct gatgaaaaag acgccttgga
    13741 tccgcaggcc ggccgtcgtc cgggcgtttg agaacttgga tcaaaccgtc gccgttctgg
    13801 gagaaaagca caagccgggc cggcggccgg ccgttttcct gctctctttt ctcatccggc
    13861 tcggaaaata catctccgtg ttcctcctcc tgttcgcggt cttgaggagc cggggcgttt
    13921 cctgggaagg cttgagcttc tggaagaccg tcctgggctt gaccggagcg gaaatgaccg
    13981 ccgtcctgcc ggtcaaggga ctggccgggt tcggaacctg ggaatccgcc tgggccgccg
    14041 ctctccaatg gatgcgtttc gacccgcggc tggcgatcct ctccggcctg gggatccatc
    14101 tcgtgacgaa cctcttcgag tatgccctcg ccctgggaag cctggccgtt ctcgccgtcc
    14161 gccgcaaacg tgatagaatg ggccgaaaat catgagcatt ttcgaagatc gctttcacct
    14221 tggaccgtcg gatttcaccg atgttctgga gaccgccctc tcccggggcg gcgatttcgc
    14281 cgagcttttt ttcgaatacc gcctccatca ttccatcctc atcgaggaac agattatcaa
    14341 ggagacggcc gaaagcgtcg ggctcgggct cggtatccgc gtctggtccg gagatcagac
    14401 cggcttcgga tacaccaacg acctttcccc ggagcggatg aagaaagccg cccgctcggc
    14461 cgccgccatc gccgtggccg gcggaacgcc ctcgcccccg gccgctttcg tttcccgttc
    14521 ccccctggcc ggcctttacc ccgtcgccga accggccgtt tccgccgacc tgggccgaaa
    14581 gatcgccctg gtccgggaag cgtacgaggc ggccctggcg gtttcgccgg cgatcaaaaa
    14641 agccaaggcc gcctggggcg actacgtcca gcatattctg atcgccaatt ccgagggatt
    14701 gatcgtctct gacgtccggc cgatggtcaa attgacctgc acggcgatcg ccgaaaaagg
    14761 cgcggtccgg gagtatgggt tttccggcgg aggcggacgg acgggactgg aagtctttgt
    14821 ctcggtccgg actccggccg agatcggccg ggaggcggcc gaggaggccc ttctgctgct
    14881 ggaggcggcc gatgcccccg ccggcgagat gcccgtcgtc ctggccgcgg gcgaggccgg
    14941 cgtcctgatc catgaggccg tcggccatct gctcgaagcc gatttcaacc gccggaaaac
    15001 gtccgttttc tggaacaaga tgggtgcccg ggtcgggacg gatctcgtca cgatcttcga
    15061 cgatccgacg attccggccg gccgcggctc ctatgccgtg gacgacgagg gaacgattcc
    15121 ccgccggacg ctcctcatcg acaagggcgt cgtctccggg ctcctgaccg accggctctc
    15181 ggccaaaatc ctcggacgcc cgctgaccgg ccacggacgg cgccaggatt attccgagat
    15241 ccccatcccc cgcatggcca acacgtacat cgccgccggg ccccatgatc cggaggaaat
    15301 ccttcgctcc gtcggccgcg gcttttatgc caagcggctg acgggcggac aggtggagga
    15361 ttcaggaaag ttcacgtttt ccgtcagcct gggctacttc atcgaaaacg gccggttgac
    15421 gcgcccggcc aagcaggcga ccctgattgg aaccagcctc ggcatcctca accgcctctg
    15481 catggccgga acggatttgg aattcggccg gcccaccggg acctgcggca aggaaggcca
    15541 gcacgtcccg atcaacgacg gttgtccgac gctgaagatc tcgtcgatga ccgtgggagg
    15601 acggggatga cccccggggc tgcggtcgac gacttgaaat ccctggccga gaggttcgtc
    15661 ctcttcggcc gagcctgcgg agccgatgaa atcgaggtcg gcctgggcga cgacaccgag
    15721 ttcgaggtcg atgtccgcct gggccggatc gaaaacctcg tcgaggccgg aacgcgttcg
    15781 atggggcttc gagtcatcaa ggacgggaaa accgctttcg cgggctcctc cgatctgacg
    15841 ccggagacaa tcgagggctt gatccggaac gcggtccggc gggcggagct cgccagcccg
    15901 gatccctgcg ccggacttcc ggattctttt tccccggcgg tcgatccggc cggcctcgat
    15961 ctttatgatc cggccgtcgc tgtcctcgat ccggcggaga aaatccgcct ggccatggaa
    16021 accgagcgga tcgctctttc cgactcccgc atcgtcaatt cccacggctc cagctgccac
    16081 acccacgagg gcgtttccgt cctgtccaat tcccggggat tcctcggcgc ctaccggaaa
    16141 acgttcgccg gattgaacgt cggtttgcaa gccggcgaga ccgatgactt ggccgaggat
    16201 ttctggtttt cctccgaaac ccacctcgac gccctggccg ggcccgagga gatcgcccgg
    16261 acggccgtcg cccggacgat ccgccatctc aagccgcgca agatcgaaac ccaaaaggcg
    16321 cccgtggttt tcgaaccgct gatgacgggt caaatcctcg ggttcctctt cggatgcctg
    16381 accgggacgg ccgtttatca gaaatcgacg ttcctcggcg agcgcctggg cgaccggatc
    16441 gggcgggagg cggtcaccgt catcgatgac gggcttcttc cccggggtct cggctcgagc
    16501 ccgtttgatt ccgagggcgt gccctcgcgg cggaccgtgg tcatcgaaaa cggcgttctg
    16561 aagtcgtttc tctgcaacac gtatgccgcc cgaaagatcg gcgcctcctc gaccggaaac
    16621 gccgacggat ccggcgtcgg cccgggcaat ttccatcttc ggccgggcgg ggacgcgccg
    16681 gaggaaatca tccggtcgac ggccaaggga ctctatctcg tccgaacgct gggccaggga
    16741 ttcaatccgg tgaccggcga catctccctg ggggcgtttg gtttatggat cgagaacgga
    16801 gaattcgctt atccggtttc cgagatcacg atttccggaa acctgggcga cttcctgatg
    16861 aatatcgaga gaatcggcag cgatttgatt ttcaacggat cgatctgcgg accgacgatc
    16921 aaagtgtcgg agatgcagat tgccggccgg tcctgatcgc accggcgccg gccgggaagg
    16981 ataaacatga ataaaatcgg acaacgagcg ggcgcccttc ttgggtcctc gattctcatt
    17041 attttaggca gcttgatcat gggcctgggg tacgccctgt tcctgatccc ccatcatttc
    17101 gtcccgggcg gcgtctcggg ggcggccatc atcgtcaatt tcctgacccg atttccggtc
    17161 ggcacgttga tcctcatcct caacatcccg atcttcgccc tcggctacaa gatcatgggc
    17221 cggaagtccg ccgtccggag cctggccgga ctgttcatct cctcgttctt catcgacttt
    17281 ttcaacgtga tgatcggcct cccgtcggct acggaagacg ccgtcctggc cgcgatctac
    17341 ggcggagtga tgctcggcat cggcttggga ctcgttttcc gcggcggagc gtcgaccggc
    17401 ggatcggaca tcatcggcca gatcttcaac aagcgtttcg gcctgtcgat cggcatgtcg
    17461 atcatgctca tcgacttttt catcatctcg ggttccgggt tcgtcttcgg cgggctggaa
    17521 gctcctctgt acggctacat cgtcctgttc gtctcctcca aggtcatcga tatggtgctc
    17581 gagggcttga gcttcacgaa gatggtgatc atcacctcga ccaagaccgc cgagatccag
    17641 gctttcatcc tcaagaccct cgaccggagc ggaacggccc tccggtcgcg gtcgctgtac
    17701 ctgaaccggg agggggaaac catcatcacc gtgatttccc ccaagcagct ggtcgagctg
    17761 cgggcgttca tcaagtcgct cgacccggac gcgttcgtca tcgtcaacga cacgtacgcc
    17821 gtcctgggaa ggggtttcaa ggctccccac gtcggctgag ggaagagcgc cttccgtctc
    17881 agtacgtgtt gaagcgggcg accgcgtcca gcgttctctt cggaacatgg aagacgccat
    17941 cggcgtcctt ccaatagcga ggcgaatccc ggtacggttc gacgacggac gtttcggccg
    18001 gatagcccaa ggccaggacg gaatcgatcc ggacgtgatc cgggattccg aagagggctt
    18061 gtattttcgg ccggtcgatc gagatcagcc agcagccggc caccccttcc ccggccgcgg
    18121 ccaaggccat gttctccacg gccgccccga cgtcgtactc gaacatcttg tcccggatct
    18181 tcgtgttgac gagaacaatg atgtaggcgg ccggctcccg gccgggcttc gggtcgccgt
    18241 cgggctggat gtaggccgcc catttcaagc aggggaaaac gccggcccgg gcggccggat
    18301 cgtcgacgac aagaaactcc agcggctgga gattggcggc tgagggggct agccgcccgg
    18361 cgtccacgaa gtccatgaga aggtcccggg aaacgggttt cggccggaac tggcggatgc
    18421 tccggcggga agcgatcaac cggcggatat tcatggcggt gtctcctttt cgagcccggc
    18481 cggtcccctc ttccgaactt tccgttccag cgaccgggcc gccggggtga cggccaggcc
    18541 gccccgcgac gtcacccgga gctcggatgg aagaatccgt cccgaggcca gcgaggcgtc
    18601 aatcgtatca tcgaggggaa ccgggttttc atagccgccg agaacgaggt cggcgcaaag
    18661 aaacgccgaa gcggcggccg cggcgttccg ggtgaaacag ggaatttcgc atcgtccggc
    18721 gaccaggtcg caaaccgagc ccatcgtatt ctgaagcgag atcgcggccg cgtcgcaggc
    18781 ttgccgggcg gttccgccgg cggcttcgac cacggcggcc gcggccatcg ccccggccgc
    18841 tccaatttcc acctggcagc cggcgatctc ggcagcgaaa gtcgcccgcc ggacgaagac
    18901 gaggccgatc gctccggccg cgaacaagga ccggaggatg tccgcccggc cggcccggcc
    18961 atcctccgcg agggtcgcgg ccacggcggc cagaacgccg gccgaacctc cggtcggagc
    19021 ggcgcagacg acggccccgc tgttgaccgc ctgcatcacg gccatggccc gggccgccgt
    19081 ccgaaccgga cgtccgcccg acggcagcgc gttccgcttt tccgcttcga gaatccgggc
    19141 ggcggccggc cggagaagcc tcaaatgaac ttgtcccgga tcaaggccgg accggaggga
    19201 tcgcttcatg acggcaaagc gccggagcat ttccgcgtcg acgtcctctc cggacaagcc
    19261 cagaacgcgg atttcgttca agcgtccggc ctcgcccgcc gtaagtcccc gccgccgagc
    19321 cagggcggcg aggtcagccg cgctccggaa caggggctcg gccgcccggg ggaagaacac
    19381 cggttcggcc cgcagccgga tgaaccgtcc gtccggtccg cggaaattca tccggaaagc
    19441 tttctttccg tcgatccaaa ccggttttcc ctcgatccga acgacggcga acgatccgcc
    19501 gccggtcgag cgggccagga tctccagtct ccggccggag gccgtccaaa ggcggacgcg
    19561 gacggcgttt ggatgaccgg cgcgtccgag gcgtccggcc tcgaaggcga tgtcgagccc
    19621 ctcgctccgg gcggtccgga ggacgtcggt gaagcgcgcg tcggtcagcg gccggccggc
    19681 aagaccgcag gcgaaggccc ggtcggcgtt ctgggtttgg taggtttcaa tgtaagaacc
    19741 cgatggatcg aaagaaacaa cggctcggac gggcggttcc cccaggagca tccggcaggt
    19801 cgcggcgatg tgataggagc cggccgtatg ggagctggag ggcccccgca tcaccggccc
    19861 gatcacgtcg ttgaagacgg acaggaacgg ccggccggat tcggatggcg cgtccttcgg
    19921 catgtccctt ctcctccctg acgtcggcgc tccctccggg acgcgggaat cccggccgga
    19981 gcgtagggcc cggattccgc cgacggtcct cacgagggcg ggccgtttca gccggacgcc
    20041 cgaagccaag tgtcagccgg gagcgggatc attggtttat ccatggcatt tcggtacccg
    20101 gccggcgtcc caacagaatc gggctccggc ttcactcccg ggatttcgga ccgcctcaat
    20161 gccggtggat atccttgtgc atgatgctcc gcatccggac gcggcgctcc tcgttcggca
    20221 tggcgtcgag gctcttgttc agaaactcat cgtcctccgc ctgcagcagg cggatgacgt
    20281 aatcgatgac gctcggcggg aacatcccgt cccgctcgta gagtccgcgc tcgctctgga
    20341 ggatccggct cgatccgacg cagctggagg gaagggcggc caggcgggag agcagctcct
    20401 tgtcggcgaa gatgtcgccc ttgacgtaca gcttctcagc cagggcccgg gccgattcgc
    20461 cgcgggtggc cttgtctttc cgggaaagag cccactcggc ggacatcgtc atcccggcca
    20521 acaagaggtg gatcagggcg cttccgtcgg gcgtgcggag ctcgacggtc tgccggccct
    20581 cgccggcccg gtacagggcg gtgtcccggg gattcaagga ccgggccaag tgccgcttgt
    20641 tcgtccaagc caagggaacg cggatcatgg cgctccggtt gagatcgctc cagcagacgt
    20701 aggtcggcgc ttcctggtgg ggaacgaggc ggagataagc cgaagacgtc gtgttgccga
    20761 aggccgtcag ggattcggcg tgacggcaga ggccgccgac cagcaggcgg gcgtccttgg
    20821 agagggcgcc gtcgggcccg gtcatgacgt tccgtccgcc gcgcttcagc tccatgtgga
    20881 agtggaggcc cgttccggcc gccccatcct cgagcttggg agcgaatgtc accgagcagc
    20941 cgttgcgcca ggcgatgttt cgaatcagcc agcggccgag gacgacgtca tcggccatgg
    21001 attcgatcgg ccggggggcg aactcgattt cgagctgctc ggccagcttg cccttgagct
    21061 cgggaaggtc gctttggacg ccgtcgatgc agccgacctc gccgtgggcg tacttgacgg
    21121 cgccgttgat cttggtgatc cggctgagaa tctcgttgac gatcggcccg gacttgagaa
    21181 agggcgggct gttgtgatag cccttctgga aaccggtcgg aaaaagatcg gatccccgct
    21241 cggaaagcag atagaactcg atctcgccca gggcgtaaag ctcgaggccg gtgttcttgc
    21301 ggaataattc ggccgcccgc ttgaggatgc tgtccggggc gaagggggcc agggttccgt
    21361 ccttggtgta atagcggcag atgaaatcca ggctgcggtc gtcgaacggg ttgagaaagg
    21421 cggaggaata gagcgggacg acgtagaggt cggaggcggc cgtttcgacc attcccttga
    21481 agatggacga gccgtcgagg cgctcccctt cggccaggat cgcctcggcc tgagccttgt
    21541 cggcgacggg gaggcggagc tccttcaacc ggccgtccag ggcggtgtaa tgaaacgtga
    21601 tccgttccag cccgcggcgg gcgatgaccc gaatgagatc ggcccgggtg aaatcctccg
    21661 gacgtttgtc gagcaggacg gagagggggg aagacagggc gaaaggatag cgggacgagg
    21721 gcatgagcga actccttggc ttatgggacg cgacgggcgt ccgaatcgag gtcgtttttc
    21781 cgtccggacc ggacggaaaa ggcattttat cggaatggcg gggctttgac aacccggcgg
    21841 gacagggggt ctccctcaat acaggagata cggccggcgc acggcctcga attcccgaag
    21901 gccgggttcg aatccgtcgg cgatctcccc cgccggcgcc cccccgagaa aggctcggcg
    21961 gacggaggcc gtcccgcaga ccttatcgaa ataatcgtcg tgaaaggcga agcggtccgg
    22021 atacatctcc cggagaacgg cgatgatatg gagcgccgtc cggatgggcc ggaagacgct
    22081 ccggtcgatg acgtggagct ggcagccgcc gcaccgctcg ccttggaact tggaaaacgt
    22141 cggagtgaac cactgctccc ggaaaaccgc gcccggcagc ccgagggcgt tgagccggtc
    22201 cgtcagggcc gccgcgtcga tccacggcgc gccgaagaac tcgaacggac gggtcgttcc
    22261 ccgcccctcc gaaacgttcg tcccttcgaa caggacctgt ccgggataga cgacggccgt
    22321 gtccagtgtc ggcatgttgg gagacggcgt cacccaagga agtcccgttt cgtcgaacca
    22381 caggccccgc gtccagccct tcaccgggat gaccgtcagc ttggccttct tctccaggaa
    22441 acgatcgttg atcaggagag ccatctcgcc gatcgtcatc ccgtggcgga ggggaatggg
    22501 ataaagcccg atgaagctgc tgaattccgg ggattcgaga accggcccct ccaggatggc
    22561 tccggtcacc gggttcggcc ggtctaagac aacgaagtcg agcccggcgg cggcgcaggc
    22621 ctgcatggcg taggccattg tcgccgcgta cgtgtaaacg cgcgtcccga tgtcttggat
    22681 gtcgaacagg aggacatcga tgccgcgggt catctccggc cggagggctt tatcctcgcc
    22741 ggtcgtatca aaggtccgca tgaacgcgtc gatacccccc aacatgtccg gctgcggctt
    22801 ttgcgacggg ccgtagagac tgaagacggg caggccggag gcggcgtcga tcataaacgg
    22861 gacgtattcc ccggcctggg cttctccccg gacgccatgc tcgggggcga acagggcgac
    22921 gagacggacg cccggaacgt tgcggatccg ggcgatcgcg ccggacaggc gtgaatcgac
    22981 gccggaagga ttcgtgatca agccgacgtt cctgccccgg atcaaatcga ggcgctcgga
    23041 aagcagcgtc tcgagtcccg gacggacctt cgaagcagcc gcctccatcg cggagggatt
    23101 cccgctccgt tcaggccggc aggcggagac cgccgcggcc gagaccagac ccaggatcaa
    23161 aacgggaacg attttccatc cggcgcgtgt ctttctcacg ttcgctcctt tccgattatc
    23221 cggatcggcg gcggtggctt ctcatattct aatggacaac cggtgtctgt acaacggcgc
    23281 cgtcaagccg atccacaaga gcgccgcgag gacggcatag atgagccggc ccggaaccgg
    23341 ggcggccagc ggaccgagga tccgctcatg aataaaaacg acggcgtcca accggacccc
    23401 gccgctggaa acggtaatga gacgcaggat tttttccaac aggaccgagc cggcgaacac
    23461 cgtgatcgaa ttcgccccaa gaacccggaa gggccaggac caacgtttcc actggagcgc
    23521 ttcgatgaga taataataaa ggccgaaaac gatcaaggcc aggccggctc cgaggaaggc
    23581 gaatgccggc gtccaaagcg gccggttgat cggcaggacg ggatgaagaa gcaggccggc
    23641 ggcggtcagg ggaagaccgg ctgaaaagac gccgagcgtt tttctgacca tcgtcctcgg
    23701 ggtcttcagc cagtccccga gcagggttcc gataagcaga acggcgacgg ccggaagggt
    23761 gctgagaagt ccttccgagt cgaacccggg cttgagaaga tgccggccga aaatcaggtt
    23821 gtcgaggtag ccgggaagat ttccgtccgg ttcgagaatc cccgccccgt ggccgggaac
    23881 gggcacaagg aaaatcaagg ccgcgtaaac ggccggaagg acggccgaga ggacaacccg
    23941 ggcggagggt ttcgtcttga ggccaaggag agctccggct gaataacaga gggcgattct
    24001 ttggagaatg ccggggatcc ggacggcgga acaatcgaag gccgggaaaa aatttagaaa
    24061 aaggccgagg agaaagaggc cggccgaccg tttcaatatt tggccgatca gagcggactc
    24121 ggcgatcccc gccctcctcc ggcggccgag ggagaacgga atgacgaccc cggcgcagac
    24181 caggaaaaag ggaaagagca gatcagcggg agtccagccg tgccaaaccg ccggttgcag
    24241 attcgaataa accgcgtggg cgtttcccgg attgccggcg atcaacatcg ccgcgaccgc
    24301 cgtcccccga aaggcatcga tggaaagtac tcgttccccg tccacgggcc cttttcctcc
    24361 ggccgcgttt cattccctgt ccagacggcc gcattataat ccatttgggg aaaaagacaa
    24421 tgtttttttt caaacggagc ctgaaattat ttcaggctcc ggaaggcggc gatccggcgg
    24481 acgccctcgg cgaagtcctc cggcgtcccg gcaaacgaca accggaggaa tccctccccg
    24541 cccggaccga aagcgatccc ggggatcgtc acgactttcg tccgaccggc caggccgagg
    24601 gcgatatcca gggatcctcc caagtccgcc cgccacgagg agacatccaa aaagatatag
    24661 aaagcgcctt ccggctcgat gaagggaagg cccaggcggc ttcgcagggt cttgacggcc
    24721 gcgttgcggc ggcggcggag ctcggccagg ttctctttcc gttcttgatc ggccgccccg
    24781 tcgaaaacga gaatcgcggc cctctgcgag ggaaccgggg cgcacatgac ggccatctgg
    24841 tggatcgccg tcaccgccgg agccagggcc gacggaacga cgcaccagcc gaggcgccat
    24901 ccggtcatgg cgcagctttt cgaaaggctg tcgacgacga tcgcgtccgg catccaggtc
    24961 cggattgatt ccgggcggcg gtctccgtaa taaagctcgc ggtacacttc gtctgaaacc
    25021 ggcaggatgc cccgttcggt caaaagtccg gccaagcgtt cgatctcggt ccggggatga
    25081 accgctccgg tgggattggc cgggctgttg agaatgacgg ctttggtttt ttcggataaa
    25141 agcggcttga tccgggaggc cttcaaaacg aatccatctt cccgggccaa cggatagaag
    25201 acgggaaggc cgccccaaag ccggacgatc cgggcgtaag tgggaaatcc gggatcggga
    25261 accaggacct cgtcgcccgg atcgacgaga acggaaaggg cgagaaaaag cgcttcctcg
    25321 gaaccggcgg tgacgcagac gcggccggga tccacgtccg ggccggcgga agccgcgatc
    25381 cgggcccgga gctccggaag cccggcgttg gctgaataac cgagaggcca gcggccgatc
    25441 ccgcgggaaa cgccgtccaa gacggcccgc ggcgttggaa aagcaagctc ccccagaccg
    25501 aggtcgacgc acgaggaatc ggccaaatcc gcgatccgcc ggagaagggt tttctccagg
    25561 ccctccgccc gggcggcgag gcgccggcct ctcatcgtcc cgccgtctct ccgtccagat
    25621 cgcggacgag ccgccggtaa atctcggccg cttcgatcaa ggcccgtttt tcgacttttt
    25681 cctccgcggt gtgggcgtcc tcgggttcgc cggggccgat catcatccgt cctccgaatc
    25741 cctcgaggta cggcgcatcc gagccgaacg gaaggatcgt cgtttcgtag ccgggccgtt
    25801 ggagaaaacg caccggggaa gattccagga cgacttcgat ctcggcccgg ccggccgccg
    25861 cttgaaacac aagatcccgg acgaccgcga ccgggacgga gcagcggatc atcaccgtcg
    25921 cccaggcttc ggaggcaagg acattttcgg ccgtccctcc cccgattttc ccgatattga
    25981 tcgtcgaggc gcccaggacc gggtcaaccg gccaggcgat cgaccggaga tcctggagca
    26041 cgtccaaaag tccatcgacc gccgatttcc cgagatgggg aaaggccgaa tgagccgccc
    26101 ggccggccgt ccgcaggcgc aggctgaaca cgcccttgtg gccgagggcc aatcgatttt
    26161 gggtcggctc cccgatgacg atgaaccggc atccgtcgct cagcttcccc gccgctcggg
    26221 ccccgtcgct gtcggtctcc tccccgacga cgagaagaat cccgaagcgt cggaagccct
    26281 cggcaaggag cgcccgcccg gcggtcatca tcgccgcgat cgagcccttg gcgtcgcagg
    26341 cgccccggcc gcggacatgg gtctcatcct cggcgccggg aagcggagga gggacggtat
    26401 ccaagtggga gcagagaacg acgccggccg gctccccggc cgaggcgaag agattcgccc
    26461 gtcccggagt aacgtcttgc cggacgacat ggaagcccgc ctcttccagc caggcggcgg
    26521 cgaaatcgac ggccggacct tccgccccgg tcaccgaggg aatgtcgatc aacgtccggg
    26581 ccagccgaat cacgtccatg aaatccatcc ttttcgacgg ccaagctgtt cgggataagg
    26641 ccgatcagcg ccggcggagc ttcccgacaa tcgtgatctt ttccggggcg acatcgaccg
    26701 cgttcttgat gatccgggcg ggcgctccgg cgacgacgac gccggccggg acgtcggaga
    26761 caacgatcgc cccggccccg acgacgctgc cgcgcccgat gcggacgccc tccagaacga
    26821 cgacgttggc tccgatcaga actcggtctt cgaccacgac cggaacccgt ccgggcggct
    26881 cgagaacgcc ggccagcacg gtcccggccc cgatatggca gtttcggccg acgatcgccc
    26941 gggctcccag gacggcgttc atatcgatca tcgtcccccg gccgacttcg gccccgacat
    27001 tgatgaccgc gcccatcatg atgacgcagt cgcggccgat cgaggccccg tcccggatga
    27061 tcgctccggg ctcgacccgg gccttgacct tgcgcaaatc cagcatggga agggcggaat
    27121 agcgggccgt gatctcggcg tggacgttcc ggattcgttt ccgtcttcgg gagagccaag
    27181 aagcaatcgt ctcgtagtct ccgaacagga tccggaaatc gcccgttccg aaggattgaa
    27241 acccggcgtt tttaaaatcg tcttcccgga acatcccttg gacatagacc cgggacggcg
    27301 tcgtcttggg gctcgaggcg atccgctcgg ccagggtccg ggcgtcccgc ccgccggtgc
    27361 ttttccgggg cttcccggtc ataaaagtcc gcgctccttc aacagggacc ggatgtactc
    27421 gcggttgccg tcaccgagtt cggacagcgg gggccgcagc ggtcctccgg gaagtcccag
    27481 gagcgaaagg gcggccttga ccgccatcgg gttggtttcc aggcggaggg cggcgaaaag
    27541 atcatagtag gcgtaatgga gggaccgggc ctcctcgtgc ctcccggcca ggctgtggtc
    27601 ggtgagctcg cgcatcactc ccggcaggat gttcgccgtg acgctgaaca tgccgctgcc
    27661 gccgaaggcc atcagcgggt aggcggtcgt gtccttgccg gtgaagatct cgaaattctt
    27721 cccccgggcg agatggatga gcttggccag atggtcgagc tcctttttcc cgtccttggt
    27781 gccggcgatg ttgccgtgat ccatgagctt ggcataggtc ttcggctcga cgttgacgcc
    27841 gacccgctcc ggggcgttgt gaatgacgat cggcacgggg cacgcgtcgg ccacccggcg
    27901 gtagaaatcc agaagcccgg cctggttggg tagaaccagg aacggggcga aaacgacgac
    27961 gtagtcggct ccgatctcgg cgaacctccg ggcccgggcg atcgtctgct cgaggttgtt
    28021 ggtgcaggtg tcgggggcga ccttggcttt tcccccggct tcctcgactg tgatttcgac
    28081 catccggtcg atttcgttct cgttcaggga aggattttcg ccggtcacgc cgagcggggc
    28141 aagaccatga acgccgccct cgatctggcg tcggaccagg accctcagac ccgcctcatc
    28201 aagggcgaaa tccttggtaa acggcgtcat gagcagcgtg tagactccct tgatcatcat
    28261 gtcaatcctc cctgtgattc ttttcggatc tcccggcgcc ccgcccgggg gaacgacgga
    28321 aaacggttct gccggagaaa ggaatcgaac cttcacgggc tgtgaggccc actggatttt
    28381 gagtccagcg cgtctgcctg ttccgccact ccggcaa
//
LOCUS       DAOOXH010000022        27643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_1509_length_27643_cov_7.827679, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000022 DAOOXH010000000
VERSION     DAOOXH010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 27643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27643
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1509_length_27643_cov_7.827679"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(524..811)
                     /locus_tag="PKV64_04305"
     CDS             complement(524..811)
                     /locus_tag="PKV64_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16338.1"
                     /translation="MPGENSTPPVRTEVLLTYDDAAFYVAFRCFDPEPAAIRAHLMDR
                     DDTDTLIQDDHISFMLDTFNDERRAFQFRVNPLGVRADRTFFFKIGYAWVM"
     gene            complement(1043..1303)
                     /locus_tag="PKV64_04310"
     CDS             complement(1043..1303)
                     /locus_tag="PKV64_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPN16339.1"
                     /translation="MVELPIDGILDLHAFDPADVRELLPEYLNACREKGIFRVRIIHG
                     KGTGEMMKTVHALLVRLAEVESFALAGSREGGTGATIVLLKT"
     gene            complement(1473..3440)
                     /locus_tag="PKV64_04315"
     CDS             complement(1473..3440)
                     /locus_tag="PKV64_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPN16340.1"
                     /translation="MKKKDRAPFITFRPSILKSAAFGVVFAAVSSAAPFLLHALPAPD
                     APPAAAAGSPPPAAPRRGSPPPSPEDGAVHAFGFQGTEFILDGKPFQILAGEMHFQRI
                     PREYWKDRLRKARALGLNTVGTYVFWNALEPEPGQWDFSGRNDLAGFIAEAREAGLWV
                     LIRPGPYVCAEWDFGGLPAWLLRIPDIKVRCSDPRYLAACGKYIEKIAGILRPLQVHE
                     GGPVLMVQIENEYGSYGNDRGYLPALKALWEKNGLRLPFSTADGAAPHMLEAGTIPGC
                     AIGLDPAASGKEFAEAEKLGRNVPVFCSELYPGWLTHWGEPWARTPAEDVLKDLRWLL
                     ENGKSFSLYVLHGGTNFGFWAGANMGKTYQPDVTSYDYDAPLNERGDPTPKYFAVRDL
                     LAQYRPAGTPPPPDLPDPLPAVDTPDVSFTGRAALFDNLPAAADCPQPRPMEMFGQNA
                     GFILYRTELSGRRSGTLAIQELHDYAVVSVDGKILGTLDRGRGESTMTIPPSNPPARR
                     LDILVEGLGRINYGPHLLDRKGITDRVTLEGMTLMSWNVVPLPMDEAFLEGLRFVNET
                     GAESGERPGVFFRGFFDLMETGDTFLDLSGWRKGVVWVNGRNLGRYWDIGPQKRLFCP
                     GPFLRKGRNTIVVFDLHRTAPAGIRGFKTPE"
     gene            complement(3465..4154)
                     /locus_tag="PKV64_04320"
     CDS             complement(3465..4154)
                     /locus_tag="PKV64_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HPN16341.1"
                     /translation="MSIGGIDFLLILPALILALIAQGMVKSTYAKYSRIGAASRITGA
                     QVARLLLGQGGAGDVTIEQTPGQLTDHYDPRKKVLRLSEGVFGSSSLAALGIAAHETG
                     HALQHRERYAPLMTRNLIYPVANIGSTLAFPLFFIGLLTAGSGTSILMDIGIWLFVGA
                     VAFTVVTLPVEFNASRRALALLKNQGYLGPQELVGAKKVLQAAAMTYVASTAMAVMQL
                     FRLLIIRGSRD"
     gene            4490..5743
                     /locus_tag="PKV64_04325"
     CDS             4490..5743
                     /locus_tag="PKV64_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN16342.1"
                     /translation="MKTISRGLLCALIILASAGLLSANQEVEVERNLAAADAFFEKSD
                     YPAAIEAYFKAAALSTKPENLSRAYFGLSLCHFYQRDMAESVRWMRKVALVDPDKRIS
                     ADAYPKPFVDLFTQVLAEARAKGTPVVSPGRVEGPPATAPQPKTVEVAPEKAEPPPAA
                     APVLPPAAGVSSAGDFWSRLAGRFEISVHFSDWTVSPVTSLFESALKDEFGEAIQTAI
                     NRELNRRYPFLIKGPFSSDLALDSEGSNFGFELRYYARGRAGTFSLGLGFEKTNIAFS
                     ASGTARQEYAPAGVAEAAASAKIETKPFATHLSFRWEIGSPAARVKPFVTFGLGLAPL
                     DGTFEYSYLGTYSYAGYQESIQDAAKKDFKALSEDIDFDIPDLIVFLQMDIGLKIEIF
                     RGLFLTGQAGLWNGFHLRGGIGYRF"
     gene            6240..6505
                     /gene="ffs"
                     /locus_tag="PKV64_04330"
     ncRNA           6240..6505
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKV64_04330"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            6674..7507
                     /locus_tag="PKV64_04335"
     CDS             6674..7507
                     /locus_tag="PKV64_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPN16343.1"
                     /translation="MKTKKTAVLILAVSFLLAGAVFIRAQEEETDVEGAKDHPLLSRM
                     KNFFISDYSARYDEHEFYISESDSKTVEGDRTRIDYQLKEGAPQPSPLQIKRNYANAM
                     KTLGAKLLHEDEDYACWTLVKGGQEIWIDVQVYNDGWNYELNIIQIAAMVQEVTANEM
                     LDALNKDGFIALYINFDTGKYDLKPESQGTIDQIIALLADNADLNVSIEGHTDNVGQP
                     AANKTLSEQRARAVRDAVVKGGVSASRVDAVGWGQERPVADNRSEDGRAKNRRVEIVK
                     K"
     gene            complement(7557..10760)
                     /locus_tag="PKV64_04340"
     CDS             complement(7557..10760)
                     /locus_tag="PKV64_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPN16344.1"
                     /translation="MEKLRNVMLAPLALALAAVFAAAAAAQDLKGPTGEEWKNPRIVG
                     AGKEAPRSFFIPYPDEAAALENDKDASARRRCLNGPWKFHWVPKPADVPAGFFKPGFD
                     ASGWKEIAVPSNWEFQGYGVPIYLDTDYEWLKAGETPDPPNLPADNNPVGCYKKTFSV
                     PADWKDRDVFLHFGAVKSAFYVWVNGRYAGYSEDAKTPAEWNITRFLKEGDNEVALQV
                     FRWSDGSYLECQDFWRISGIERDVDLLARPRVRIRDFWARADLTADGRNGFLDVSVEV
                     TGRPGGAGGLSTVEITLLDGQGGTVFSAGAPVTTARGKETAIARIARNVTAPRLWTAE
                     TPNLYTVVLALKDASGRTLEATSTRVGFRNVEIVDGRLLINGAPVYLKGVNRHEHDPR
                     TAHVVSEESMIRDIRLMKQFNINAVRTCHYPDDPLWYDLCDAYGLYVIDEANIESHGM
                     GDGPESLAHKPEWGPAHLDRTIRMVERDKNHASVIIWSLGNEAGDGLNFEATSAWIHG
                     RDPSRPVQYEPAGTRPHTDIVCPMYARIETIEAYGLKKQTRPLILCEYSHAMGNSNGN
                     LQDYWDVIEKYPNLQGAFIWDWVDQSIETKDPNGRTYYAYGGDFGPPEIPTSRNFCCN
                     GLVNSEREPHPGLWEVKKVYQSIAFRKTETADGRTALEVRNGYAFRGLDGFVIRWELA
                     SGETGAPPVAAGEFPNPAIRPGGSAVFPLEMPTVGIVPGREYFLNVRAEYARDEPLIP
                     RGHVAAAGQFPVGLVRPGPAVKAADMRPVTLGQDGTKVTVGGSGFSASFDRTTGMLTS
                     YVFRGKEFVRTGLEPHFWRAPTDNDFGNGFPERHGIWRKAGDHRVLEGFEARAESPSA
                     VRIEARYALTDVPAKYRVTYRVLGTAEIIVSVHFVPEKEGLPEMPRLGMKMTLPREFA
                     HVRWFGRGPHESYADRKTSAFVGTYGASVFETLIPYVSIQEYGNRTDCRWALLADRTG
                     DGLLIAGYPQFDFSALPYTAEDLTQEKRGQKHPQEIPKRDYVCLNLDYGQMGVGGDTS
                     WGAREHPQYRLAAKEYAYSFRLRGITAADDPAALARE"
     gene            complement(11033..11932)
                     /locus_tag="PKV64_04345"
     CDS             complement(11033..11932)
                     /locus_tag="PKV64_04345"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPN16345.1"
                     /translation="MSKKFQGIFPALTTPFDGEDLAVHRFRENIAKFNAFDLSGYVVL
                     GSTGECVLLSNDESERLIRAARETAAPDKLVIAGTGQESTKLTIAFTNRMAAAGADAA
                     LVRPPCYYKAKMSRDVLRGHYLAVADGAKTPVIVYNIPQNTGVTIDPELLIELSTHPN
                     IAGLKESSGILSYLGKIIPHVPEDFSCLVGSGFVVLPALVMGASGAILAVANAAPAAC
                     LDIYRLFKEGRIDESAQRQLDVTPLNKAAMETYGLPGLKYAMDIQGFYGGPVRRPLLP
                     LPEPAKPEIEALVSDLRTRLSSS"
     gene            complement(11850..12347)
                     /locus_tag="PKV64_04350"
     CDS             complement(11850..12347)
                     /locus_tag="PKV64_04350"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HPN16346.1"
                     /translation="MITLSRERFQKLVAEAIDGLPRSFREKIENVAVLVEDFPSAEIV
                     EDMGLRSPWELFGLYHGIPLDKRGSYYGNIPPDVILIFQKPLERWAGTEDEIRDEVRS
                     TVIHEVGHYFGFDEADLRRAERESRRSESPADEDNIDEQEIPRDLPRPHHPVRRRRPR
                     RPPLP"
     gene            12471..13748
                     /gene="serS"
                     /locus_tag="PKV64_04355"
     CDS             12471..13748
                     /gene="serS"
                     /locus_tag="PKV64_04355"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPN16347.1"
                     /translation="MLDPKFVLENAGLVMDKCAQRGVRIDLSEFLRLSEARKETLREV
                     EDLRSCRNKASEEVAGLKRSGRDAGALIAETKLVGERIKTLEDGLKPLEERIRTTLLN
                     IPNLPDESVPVGADASGNVEVRRAGEPREFDFEPLAHWDLGTGLGILDFERAAKIAGS
                     RFTVSLAAAARMERALINFMLDIHTRERGFTEILPPFIANEASLTGTGNLPKFAADLF
                     KLEDYPWYLVPTAEVPLTNLHRDEILDGAALPERLAAYTPCFRSEAGSYGKDIRGLVR
                     QHQFNKVEMMVFSLPENSMDELESMTAGAEEVLKRLGLTYRVVALCTGDMGFASRKTY
                     DLEVWMPSRRGFMEISSCSNCGDFQARRSNIRFRRDPKAKPEFVHTLNGSGLAVGRTV
                     AAILENFQLDGGSIDVPQALRPYMGGLTRIARS"
     gene            13797..13884
                     /locus_tag="PKV64_04360"
     tRNA            13797..13884
                     /locus_tag="PKV64_04360"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13831..13833,aa:Ser,seq:tga)
     assembly_gap    14044..14143
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14433..16820
                     /locus_tag="PKV64_04365"
     CDS             14433..16820
                     /locus_tag="PKV64_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HPN16348.1"
                     /translation="MAVSVSSDGDIIDVGDGFYFEKDIVIDRCVSLRSRRLFGAVISG
                     SDNEDSNSAVLWVRSECEISGFIIKNCYHGIRQRGSLDADWKASDLGFMDCTYGISID
                     DRTGRVGSVEVRNVIFDRGLTALAINDGRAIDARRVFISRARQAVSAFDYDRCFLDEI
                     ILWNVFFFFDPICKDNDLIQSGPSIFKFHSWDPPLLLNEMSRRFDGLFARSPKPGARD
                     EDNRRRKAVLAVVLGESFLEAGDPRTATRRFQEAAILTSRDEVAEIQWRIPWGMARAA
                     EAAGDGKRAVGHYRKAVAEVDRIGLGIPLPDLLADFRREKIDLYDAFIKCLVAAEARE
                     PGLGWAEEAFDVAEQSKARSLLSSLLAFGEGVENAGREARKKADLLAFRLSRCQLELQ
                     DPDLPPERRTALLEKLKRIEAERHNFFLQLGRLLPPEPRGTFIDTLSLDQVKHGLLGP
                     ADALVEYIVGKTASYAFLVTPNGFDVAVLPPEFQLKALVEGYLEFLTFPAESEFRGWS
                     GGHRLWEILLGPFEDRLGSVDALIISPDGPLHHLPFETLVSDPVRRRFAVESHEFRYG
                     HSASSLERLRRRALPSARPMDFLGVAATSRIPAISFETGKRINFPALPNTAREIQKIG
                     RLFPPERTTILAGAEDLEAAFKAASLDAYGIIHFAAHGWIDDEEWMRSALLLGTDGSG
                     GEDGFLQPAEIRRLPLRADLVVLSGCETAGGRLERGEGIRGLAQAFLKAGARSLVISQ
                     WSIEDRVAVDFMEAFYRGVAKGYSYASALRRAKLAFLESTRRHPYYWAPFVCLGP"
     gene            complement(16825..17520)
                     /locus_tag="PKV64_04370"
     CDS             complement(16825..17520)
                     /locus_tag="PKV64_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPN16349.1"
                     /translation="MNKAHHEKRKSNGGWSPCPSPETLAKFFRNELSDGERESLIDHL
                     LVCPECRAKIEVLDEIRQDVRARKRDFRNLVRRSRREARLARGRQTSALGRFPRRTRA
                     WAAAALLFAAAGAVTYSVFSSRAPIRVVRSAGGEAMTLRRPLVLSDGKTILFRWETPP
                     GGENYWFRLIDDSLNDIVPKTSLKTALYVLHMEKLATRIVPGKTYIWTVEAFDDMTKF
                     IARSEAVFAYQGK"
     gene            complement(17429..18046)
                     /locus_tag="PKV64_04375"
     CDS             complement(17429..18046)
                     /locus_tag="PKV64_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPN16350.1"
                     /translation="MKSGGRIWTERLIAEIDQSVRTIVARKFPDISFADREDVLQEVQ
                     FKIWKMLTSGKKIDRLESYLWKVVFTTTLAVIGDKLKAVSLDDHGGGANGPVPGWKSA
                     DPAFEEMERRELLDILVGRLSQKRRLVVKCHLAGMDLEKTAAHLGWTVPKVRHLLYRS
                     IKELRKFCLASALEDNEQSASRKEEVERRMVPVSESGDAGEVLPE"
     gene            complement(18387..19427)
                     /locus_tag="PKV64_04380"
     CDS             complement(18387..19427)
                     /locus_tag="PKV64_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16351.1"
                     /translation="MKMKRAIAGPVLAVLLAALPLGAQTNDLFDDTPAVPLNHPEAVL
                     VGGEVGRETSVVLASLPLRSMAAKEAVRGDDGRLVFLGAYRYDGYSLADILDRVTVEK
                     KNSGEYSRIIDVYVEVANEAGEKAVFSWGEIYYPADRHRIILATAVSRLVPSLTKDLW
                     PLPSESKLVAGSDLLSIRNISRPSRITIRSLDMGMSGIADGKGEASSKTNLRLCLNGV
                     LIGELSRLPEKSAAYEYPAVFYGRGKGFLGIETFRGVALREVLSGKYPAADRAMKEGL
                     VAVIASDGYRAAFSLAEIADRNDFREILLMAKENNSGESGGFALFPAGDFFSDRAVKG
                     IRTIDLRVPLHD"
     gene            19646..20524
                     /locus_tag="PKV64_04385"
     CDS             19646..20524
                     /locus_tag="PKV64_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPN16352.1"
                     /translation="MSRNNSGRLISAVVCFLGALTACRAAGPAGRTVDVGGHRLYCEL
                     HGRGTPAVVIDVGAGESFKDWEAVVAALSPETAVVVYDRAGYGRSQAGPLPRDAKAEA
                     ADLEALLRKAEIPGPYVLVGHSLGGLNVQRFAHDFPADVAGLVLLDPPPRGWFAGEAF
                     PGLKRMFRAAAEDLAAQAEAAKKSSDGDERMMASYLEAVASEHAAMFGTTASQVREAA
                     AFGDLPLIVVAAGRPNPAFGEEAGAFQAYWIEENRALSRLSRKGEFILAENSGHRLHR
                     DAPDTVLRAIRKLAGR"
     gene            complement(20557..21369)
                     /locus_tag="PKV64_04390"
     CDS             complement(20557..21369)
                     /locus_tag="PKV64_04390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN16353.1"
                     /translation="MAINVDSRGRTVEESVVLAMDGTDASLYPYLPYILQDIWQIGAS
                     PDLIIQVLRRRLPRKAGFRLLDLGCGKGAVSIPVAAAFDCSCLGIDAVEAFIAEARAR
                     AAEHGVDARCRFEVGDIREEIKGLAGFDVIVLGAIGPVFGDYRETLTTLAPCLKPEGL
                     FIVDEGYFHDASSSTHPIIRKKSVILREIEEAGMELVEEAVISEEDLKESDDDIFAKL
                     KNRCLELAAAVPDKKDLFLDYVRRQLEENRVLETEAVCATLVIRRKKPPAEA"
     gene            complement(21426..22271)
                     /locus_tag="PKV64_04395"
     CDS             complement(21426..22271)
                     /locus_tag="PKV64_04395"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPN16354.1"
                     /translation="MRPCFSRIALILPAAVLGLSSSALPALSPPARPQAAASAPQAAP
                     PPGEEFTTPSGLKYRILKRGDGPVTEPGKEIGLHGIGSFPDGTVFWNSRDPENPFYFI
                     LGLDPVIPGCAEGVALMRVGDRFLFTMPPELGYGENGSGEIIPPNAVLIFDYEILSVE
                     TPKTPIAATLYQAIREKGISQAVSLYLWLKKDKGGEYNFREDQLNTLGYKLLADGKIK
                     DALAIFELNAVAYPDSYNVYDSLAEACLADGQKEKAIANYEKSLALNPKNANAEAMLK
                     KIKGE"
     gene            complement(22482..23744)
                     /gene="kbl"
                     /locus_tag="PKV64_04400"
     CDS             complement(22482..23744)
                     /gene="kbl"
                     /locus_tag="PKV64_04400"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPN16355.1"
                     /translation="MYQRIKYDFAQELVKIKSSGLFKDERTILSAQKPEIRVEYPAGS
                     DPRNVLNFCANNYLGLANHPKILEAARKALDEHGFGLASVRFICGTQDLHKILEQKIA
                     EFFHTEDTILYSSCFDANGGLFETLLGVEDAVVTDALNHASIIDGIRLCKAKRFIYDH
                     ADMASLEKALKKAREGMDVWDGPGLDKEPVPARNIMIITDGVFSMDGDVADIRRIVEL
                     GNTYDTLVVVDDSHGTGVLGATGRGSVEEAGMLGQVDIITSTLGKAMGGATGGFVTGH
                     REIIEILRQKSRPYLFSNTIPPVIAAAGIAAFDLLAQGQELREKLRDNTVFFRGAMKA
                     LGFDIIDGSHPIVPVMFRKFDNDARLAQQMARELYAEGIYVVGFVYPVVPKGQSRIRV
                     QISAAHSREQMERAVAAFEKVGRKLEVI"
     gene            24055..24552
                     /locus_tag="PKV64_04405"
     CDS             24055..24552
                     /locus_tag="PKV64_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPN16356.1"
                     /translation="MQFEGWIKRDAQGSAGLRRLGKGAGRTISAVLAHTADTTVLFPA
                     AGLIWLFAGEPWKSWAWKAGLALLVGTLATGILKLVFKRRRPDGRWGAFDRLVDPHSF
                     PSGHANRTAVLAVLAALYAPPSAAAAVILWALAVGLSRVVLGMHYLSDVIAGWLVGAA
                     VGLFF"
     gene            complement(24734..26149)
                     /locus_tag="PKV64_04410"
     CDS             complement(24734..26149)
                     /locus_tag="PKV64_04410"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HPN16357.1"
                     /translation="MSLKKLVVLVIIIIVAVIVAVSCGTRSVMRGAFREDRAGWSFVR
                     LEGASREIGYQHGRLLAAEIDDVLKTFAFYLKKETGRDWAFYRGAAEKVLWPRLERPI
                     RMEIEGLAEGLRAGKSGLKYDRFDIAALNGIIELAWYYEPALRMKRAEGGAGEAATKP
                     PLRCSAFIATGGWTADGRIVMGHNTWDDYLIGERWNVILDIVPDTGRRMLMDAIPGFI
                     HSGSDFVVNEAGLVYTETTISGFAGFDEKGVPEFARARTAAQYAASIDDFIRIMTTDG
                     NGGYANDWLVGDVKTNEIARLELGLKNHRIWRTTDGWFAGANFPLDEKLIAEETTFNP
                     DDADQSVYIRRDRWEQLMAENKGKIDVEKAMEFLGDHVDARSGEARPSARTLCGHIDR
                     DPAGAPEADWGGFSPSGAVQAKVTSAGLAGEMKIWVRMGHPCGEEFLAAPFAAAHPEY
                     AWQLPYLRDMKANPWVLFQGR"
     gene            complement(26196..26384)
                     /locus_tag="PKV64_04415"
     CDS             complement(26196..26384)
                     /locus_tag="PKV64_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010476276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HPN16358.1"
                     /translation="MPEKKKFLLRLDGDLYEALEKWAADDLRSVNAEMEFLLKEAVRK
                     AGRLKDSSNAARPGREER"
     gene            complement(26392..27270)
                     /locus_tag="PKV64_04420"
     CDS             complement(26392..27270)
                     /locus_tag="PKV64_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPN16359.1"
                     /translation="MIDIKEKRAFHVSGWAVVAIDIILIVYAVVYFIKLLGEPPMTAA
                     FFGRLGALLLVGLSCGGFFVLHPNEAMVFTFVGRYFGSVRTAGFHWTNPFCAKHRVTL
                     RVSNLVSEKIKVNDACGNPIEIAAVVVWHVIDTAKARFNVDNYKNFVAIQSETAVRQL
                     ASHYPYDSHDKDQESLRTDPDKIAAKLREQVQERLAVSGVNVVEARLMHLAYAPEIAQ
                     VMLRRQQAEAIIAARQKIVDGAVGMVDMALKGLENAGVVHLDQDKKAAMVNNLLVALV
                     SESETTPIINTGTLYT"
     gene            27486..27579
                     /locus_tag="PKV64_04425"
     tRNA            27486..27579
                     /locus_tag="PKV64_04425"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27520..27522,aa:Ser,seq:gct)
BASE COUNT         4963 a         8351 c         8866 g         5363 t
ORIGIN      
        1 gggccaggac gatcaggccg agaaccagca tgacgacgaa cttcggccgg cccaggacgg
       61 cccagaagga agccgcccgg cgtcccgcgc cctgctgggc ggccaagggc gaggcgaaca
      121 gcaggaagag aaagagagat gcgggaattc cccagcgttt cattttcatg tcgtcgcctc
      181 ccgaaatgaa ttcaagcggc gctattttat aaaatgaaat cgagagacac aagcttattc
      241 actctatttc ggctcggata aggagcccga tcgccggcct tggctgcgcc ggcgattaaa
      301 aacagctcct tccccccgcc ggcttcaagc cttcttggat gccggcatcg cccgtgtcgc
      361 gcgtcgttcc ggaaagacat ctcctgattc ggccgcggac ccggcaaaca tcgggaatgt
      421 cctcattcgg atgttgttcg ccccggccgt catcgcttcc cagattggcc gtggccagat
      481 ggaagaaagc cgatcgccct ccatctcgat cgtccgcccc gcctcacatc acccaggcgt
      541 aaccgatttt gaagaagaac gtccggtcgg cccggacgcc gagcgggttg acccggaact
      601 ggaaggccct ccgctcgtcg ttgaaggtgt ccagcatgaa gctgatgtgg tcgtcctgga
      661 tgagcgtgtc ggtgtcgtcc cggtccatga gatgagcccg gatggccgcc ggctcgggat
      721 cgaaacagcg gaaagccacg tagaaagccg cgtcgtcgta agtaaggagg acttcggtcc
      781 ggacgggggg agtcgagttt tcgccgggca tccattcgaa ggcgatgtcg aagcgggccg
      841 ccttgttccg gccgtctccg gcaaatcaga aaacgccatt cctcttcatt ttatttgggc
      901 ggggaggaat gatcgagcct gttcgttcgg gccgtcaaaa cctccgcacc ctccccccgc
      961 aacccctggc tcgaaaaccc gatcatccgc ccgcccggcg ttcgaacgcc ggcgaaatga
     1021 ggcgaatagg tattggggct ctctatgttt tgaggaggac gatcgtcgct ccggtcccgc
     1081 cctcgcgcga accggcgagg gcgaaagact cgacttcagc gaggcgcacc aggagggcgt
     1141 ggacggtttt catcatttcg ccggtcccct tgccgtggat gatccggacc cggaagatcc
     1201 ccttttcccg gcaggcgttc agatattccg ggagaagctc ccggacatcc gccggatcga
     1261 aggcgtgaag gtcgagaatc ccgtcgatcg ggagctcgac catgtcgggg atcgcgccgt
     1321 ccgggccgct tccgccgggg cggggagcgc cgccgaagcg cacgaccgct ccgcaatcgc
     1381 acgtcacggc ctggccgaat tgaaaaagcg tgacgtcgta ctgatgcccg cactcgggac
     1441 aggaaacggc catgggcctt cgctctcctc cttcattccg gcgttttgaa tccgcggatc
     1501 ccggccgggg ccgtccggtg caggtcgaag acgacgatcg tgttccggcc cttccggaga
     1561 aagggccccg ggcagaacag gcgtttctgc ggcccgatgt cccagtagcg gccgagattg
     1621 cggccgttga cccagacgac gccttttctc caccccgaga ggtcaagaaa cgtatcgccg
     1681 gtttccatca aatcgaaaaa tccgcggaaa aagacgccgg gccgttcgcc gctttccgcg
     1741 cccgtttcgt ttacgaaccg caatccttca agaaacgctt cgtccatcgg cagcggaacg
     1801 acgttccagc tcatcagcgt cattccctca agcgtgaccc ggtcggtgat gcccttccgg
     1861 tcgagaagat gcggcccgta attgatccgg cccagcccct cgaccaggat gtcgagcctc
     1921 cgggcgggcg ggttggacgg gggaatcgtc atcgtgcttt ctccgcggcc gcggtcgagc
     1981 gttcccagga tttttccgtc gaccgaaacg acggcgtagt catggagctc ctggatcgcg
     2041 agcgttccgg accgccggcc ggagagctcc gtccggtaga gaataaagcc ggcgttctgg
     2101 ccgaacattt ccatcggccg cggctggggg cagtctgcgg ccgccggcag gttgtcgaag
     2161 agcgcggccc gtccggtgaa gctgacgtcc ggcgtgtcga cggccgggag cgggtcggga
     2221 agatccggcg ggggaggcgt cccggccgga cgatattgag cgagcaggtc ccggacggcg
     2281 aaatatttcg gcgtgggatc gccgcgctcg ttgaggggcg cgtcgtaatc atagctggtc
     2341 acgtccggct ggtacgtttt ccccatgttc gccccggccc aaaagccgaa attcgtccct
     2401 ccgtggagga cgtagaggct gaaggacttc ccgttttcga gcagccaccg gaggtccttg
     2461 agaacgtcct ccgcgggggt cctggcccag ggttcgcccc aatgggtcag ccagccggga
     2521 taaagctcgc tgcagaagac cgggacgttc cggccgagct tttccgcttc ggcgaactcc
     2581 ttcccgcttg ccgccgggtc gaggccgatg gcgcagccgg gaatcgtccc ggcctcgagc
     2641 atgtgcggcg cggccccgtc ggccgtggag aaaggaaggc ggaggccgtt cttttcccag
     2701 agggccttca aggcgggaag gtacccccgg tcgttgccgt agctgccgta ttcgttctcg
     2761 atctggacca tgaggacggg gccgccctcg tggacctgga gggggcggag gatgccggcg
     2821 atcttttcga tgtatttccc gcaggccgcc aggtagcggg ggtcggagca ccgcaccttg
     2881 atatcgggga tgcggaggag ccaggccggc agccccccga aatcccattc ggcgcagaca
     2941 tacgggccgg ggcggatgag aacccacaag cccgcttcgc gggcctcggc gatgaagccc
     3001 gccaaatcgt tgcgtccgga gaaatcccat tgtccgggct cgggctcgag agcgttccag
     3061 aacacgtatg tgccgaccgt gtttaacccc agggctcgag ccttccgcag gcggtccttc
     3121 cagtattccc gggggattct ctggaaatgc atctctccag ccaggatttg aaacggcttg
     3181 ccgtcgagga tgaattccgt tccttggaag ccgaacgcgt gaaccgcgcc gtcctcgggg
     3241 gagggcgggg gggatccgcg gcgaggggcg gcgggcggag gcgagccggc cgccgcggcc
     3301 ggcggggcgt cgggcgccgg aagggcgtga aggaggaacg gcgccgccga tgaaaccgcg
     3361 gcgaagacga cgccgaaggc ggcggattta aggattgagg ggcggaacgt gataaacggc
     3421 gctcgatctt tttttttcat cgaaaggaga aacgcgtctc cgaatcaatc gcgcgatccg
     3481 cggatgatca gcagccggaa caactgcatc acggccatcg ccgtcgaggc gacgtacgtc
     3541 atggccgcgg cctggaggac tttcttggct ccgaccagtt cttgggggcc gaggtagcct
     3601 tggtttttca agagagcgag ggcccggcgc gaggcgttga actccacggg aagagtgacg
     3661 acggtgaagg cgaccgcccc gacgaagagc cagatcccga tgtccatcag gatgctcgtt
     3721 cccgagccgg ccgttagaag cccgatgaaa aacaggggaa aggcgagagt cgagccgata
     3781 ttggccacgg ggtaaatgag gttccgggtc atcagcgggg cgtagcgctc gcggtgctgg
     3841 agggcgtgcc cggtttcgtg ggcggcgatc ccgagggcgg ccagggagct gctgccgaac
     3901 acgccttccg acaagcggag gacctttttc cggggatcgt aatggtcggt gagctggccg
     3961 ggcgtctgtt cgatggtgac gtcgccggct ccgccttggc cgagcaggag gcgggccacc
     4021 tgggccccgg tgatccggga ggccgcgccg atccgggaat atttcgcgta agtgcttttc
     4081 accatgccct gggcgatcag ggccaggatc agcgccggaa gaatcagcag gaaatcgatg
     4141 ccgccgatag acatgggttt tctcctttcg cggcgagata ttaaagatag gcgagggagg
     4201 agggattgtc aacggccgat gccccggtcg ggcatggaga ggcgacggct ttcgttgatg
     4261 aggcttcccc cgttcccgag gaaacgtccg ccccgacgat tccggcgccc gccgggcatc
     4321 gtgtcggttc atggcgattc tctcaggccc gtccgcttgc ttcaaaagcg gcctgatcgt
     4381 tggcggaaaa aggggggatg attgctcggc gatcactccc gggacgcttc cggtttgaca
     4441 aatccgcccg gggaacttat gattttctct ttaaaaggaa gcattcctga tgaaaacaat
     4501 atcgagagga ctgctttgcg ctcttatcat cctggcctcg gccggcctgc tttcagccaa
     4561 ccaggaagtc gaggtcgagc ggaatctggc cgccgcggac gcgttttttg aaaaaagcga
     4621 ttatcccgcg gccatcgagg cgtacttcaa ggccgccgct ctttcgacga agccggagaa
     4681 cttgtcccgg gcgtatttcg gcctgtcgct ttgtcatttc taccagcggg acatggccga
     4741 atccgtccgg tggatgagga aagtcgctct cgtcgatccg gacaagcgga tctccgcgga
     4801 cgcctacccg aagccgttcg tcgatttgtt cacccaggtc ttggccgaag cccgggccaa
     4861 ggggacgccg gtcgtctcgc cgggaagagt cgagggcccg ccggcgacgg ctccgcagcc
     4921 caaaaccgtc gaggtcgcgc cagaaaaggc cgagccgccg ccggcggccg cccccgtcct
     4981 ccctccggcc gcgggcgttt cctccgccgg cgacttctgg tcgcggctgg ccggccggtt
     5041 cgagatcagc gtccattttt ccgattggac ggtcagcccg gtgaccagcc tttttgaaag
     5101 cgccctgaag gatgaattcg gggaagcgat ccagacggcg atcaaccggg agctcaaccg
     5161 ccggtatcct ttcctgatca aaggcccgtt ctcctcggac ctggccctcg actccgaggg
     5221 ttcgaatttc ggcttcgagc tgcggtatta tgcccggggc cgggccggaa cgttcagcct
     5281 gggcctggga tttgaaaaaa cgaacatcgc cttcagcgcg agcgggacgg cccggcagga
     5341 atacgcgccg gccggcgtgg ccgaggccgc ggcctcggcc aaaatcgaga ccaagccgtt
     5401 cgccacccac ctcagcttcc gctgggaaat cggctcgccg gcggcccggg tgaagccgtt
     5461 cgtcacgttc ggcctcggct tggcccctct cgacggaacg ttcgaatatt cttatctcgg
     5521 gacttattcc tatgcgggat accaagagag cattcaggac gcggccaaaa aggattttaa
     5581 ggcgctttcg gaggacatcg atttcgacat ccccgacctg atcgtctttc ttcaaatgga
     5641 tattgggttg aagatcgaga ttttcagagg attgttcctc acgggccaag ccggcctctg
     5701 gaacggattt catctccggg gcggaatcgg ctaccggttt tgatctttcg gggaacccgc
     5761 gccccgtgaa ttcttctctc ccggcaggaa aaatcatttc ccttttccaa acgcggccgc
     5821 gggcggcgga cagccttgca taaagcgttt tgaccgccgg tgatgcgcgg aaatcacacg
     5881 gaaaaaatcc gaaggcaaag aagacgtccg tcggcttgtt gagcccgcga cgggcggcgt
     5941 tcccggcgct tgtcatgatc tgggtcagag acatggtgga cgatctctca tatccgacct
     6001 gtcgccgctt tcatatcccc ccgacgactt tccacaagcg gccggggcgg ttcgatcccg
     6061 atgatctaac gaccttggag gacgagtcga agcggcccca tcaggctctg ggctctctaa
     6121 cccccttgga acggtgccgc cggaaatatc aatcccccat accccgggag gggcccgcca
     6181 tgaagtgccc cggtacgcgt cgctgtgttc cgggacgaat tgcgctataa tgaacgcggc
     6241 cgtgcacgac ggggagctag cggtgccctg tagcccacaa cccgctccag tggggtcgat
     6301 gcccgcgccg aggcgagact ctgtccggcc ggactttcgt ggacggtgct gaaggccgga
     6361 gcgccgagca aagggcggcc tgccaacccc gccagggccg gaaggcagca gcggtaacag
     6421 gaaggccctt gtgtgtcggt ccactccgcc ggagccgttt atgcaatgga cgcggggagg
     6481 gtcgtcgtcg gagccgggcg cacggctttt tatttcccgg cgcttttttg ccggaggccg
     6541 ccgcggccgg ggccgcggcg gggcgtcaaa ggggggattg cccgggaaag acggggattg
     6601 ccccgccgga aaggcgcggc tataattgta aacaagcaag gaccacgggc accatcaaaa
     6661 aggaggagat cccatgaaaa cgaaaaaaac cgccgttttg atcctcgccg tatcttttct
     6721 tctcgccggg gcggtcttca tccgcgccca ggaagaagaa acggatgtcg agggcgccaa
     6781 agaccatccg ctcttgtcgc ggatgaaaaa ttttttcatt tctgattatt ccgcccgata
     6841 cgacgagcac gagttttaca tcagcgagtc cgattcgaaa accgtcgagg gagaccggac
     6901 ccgcattgac taccagctca aggagggcgc cccgcagccc agcccgctgc aaatcaagcg
     6961 caactatgcc aacgccatga aaacgctcgg ggcgaagctt cttcatgagg acgaggatta
     7021 cgcctgctgg acgctggtca agggcggcca ggagatctgg atcgatgttc aggtatataa
     7081 cgacggctgg aactatgagc tgaacatcat ccagatcgcg gccatggtcc aggaagtcac
     7141 ggccaacgag atgctggatg ctttgaacaa ggacggattc atcgccctct acatcaattt
     7201 cgacaccggc aaatatgacc tcaagcccga gtcgcagggg accatcgacc agatcatcgc
     7261 cctcctcgcc gacaacgccg acctcaacgt gagcattgag ggccataccg acaacgtcgg
     7321 ccagcccgcg gccaacaaga ctctctccga gcagcgggcc cgggccgtcc gggacgccgt
     7381 cgtcaagggc ggagtgagcg cctcccgcgt ggatgccgtc ggctggggcc aggaacggcc
     7441 cgtcgccgac aaccggagcg aggacggccg ggcgaaaaac cgccgggttg agatcgtcaa
     7501 gaaataagtc gacggcgggg acgccgcccc gtcgggggga agcgtccccg ctttttttat
     7561 tctcgggcta gagcggccgg gtcgtcggcg gccgttattc cccgcaggcg gaaggaatac
     7621 gcgtactcct tcgccgccag ccggtactgc gggtgctccc gggcgcccca ggatgtgtct
     7681 ccgccgactc ccatctgccc gtaatccaaa ttcagacaga cataatcccg cttcgggatt
     7741 tcctgcggat gcttctggcc gcgcttctcc tgggtcaagt cctcggctgt gtaggggagg
     7801 gcgctgaaat cgaattgggg gtagccggcg atcagcaacc cgtcgccggt tcggtcggcc
     7861 agcaaagccc agcggcaatc cgtccggttg ccgtattcct ggatgctgac gtaggggatg
     7921 agggtttcga atacggacgc gccgtaagtg ccgacgaagg ccgatgtttt ccggtcggcg
     7981 tagctttcgt gcggtccccg cccgaaccag cggacgtggg cgaattcccg gggaagggtc
     8041 atcttcatcc cgagacgcgg catctcgggc aggccttctt tctccgggac gaagtgaacc
     8101 gagacgatga tctcggccgt gccgaggacg cggtaggtga ccctgtattt cgccgggacg
     8161 tccgtgagcg cgtatcgagc ctcgatccgg acggccgacg ggctttccgc cctggcttcg
     8221 aagccctcca ggacgcggtg atcgcccgcc ttccgccaga tcccgtgacg ctccgggaag
     8281 ccgttgccga aatcgttgtc tgttggagcg cgccagaaat gcggctcaag cccggtccgg
     8341 acaaactcct tgccccgaaa gacataggac gtcagcatcc cggtcgtccg gtcgaacgac
     8401 gccgagaagc cggagccgcc gacggtgacc ttcgtcccgt cctgtccgag ggtcaccggc
     8461 cgcatgtccg ccgccttgac cgccgggccg ggacggacga gcccgacggg gaattgcccg
     8521 gccgcggcga catgtccccg cggaatgagc ggttcgtccc gggcgtattc cgcccggacg
     8581 ttgagaaaat attccctccc cggaacgatt cctaccgtcg gcatctcgag cggaaagacc
     8641 gcgcttccgc ccggccggat cgccggattc gggaattcgc ccgcggcgac gggcggcgcg
     8701 cccgtctctc cgcttgccag ctcccaccgg atgacgaacc cgtccaggcc gcggaaggca
     8761 tagccgtttc ggacttccag cgccgtccgt ccgtccgccg tctccgtttt ccggaacgcg
     8821 atggactggt agaccttctt cacttcccac agtccgggat ggggctcccg ttccgaattg
     8881 accaggccgt tgcagcagaa attgcgcgac gtcgggatct ccggcggccc gaaatccccc
     8941 ccgtaggcgt aatacgtccg gccgttcggg tccttggtct cgatgctctg atcgacccag
     9001 tcccagatga aggcgccctg gaggttcgga tatttttcga tcacgtccca gtagtcctgg
     9061 agattgccgt tggaattgcc catggcgtgg gagtattcgc acaggatcaa ggggcgcgtc
     9121 tgctttttca ggccgtaggc ctcgatcgtc tcgatccggg cgtacatcgg gcagacgatg
     9181 tcggtgtggg gccgcgtccc ggccggctca tattggaccg gccgcgacgg gtcgcgcccg
     9241 tgaatccagg ccgacgtcgc ctcgaagttg aggccgtcgc cggcctcgtt gcccagcgac
     9301 cagatgatga ccgaggcatg attcttgtcc cgctcgacca tccggatcgt ccggtcgaga
     9361 tgggcggggc cccattccgg cttgtgggcc aggctttccg ggccgtcgcc catgccgtgg
     9421 ctttcgatgt tggcctcgtc gatcacgtaa agcccgtagg cgtcgcagag gtcgtaccag
     9481 agcggatcgt ccgggtaatg gcaggtccgg acggcgttga tgttgaactg cttcatcagg
     9541 cggatgtccc gaatcatgga ctcctcggaa accacgtggg ccgtccgcgg gtcgtgttca
     9601 tggcggttga cgcccttgag atagaccggc gcgccgttga ttaaaagacg cccgtcgacg
     9661 atctcgacgt tccggaagcc gacccgggtg ctcgtcgcct ccagggtccg cccggaggcg
     9721 tctttgaggg ccaggacgac ggtgtagaga ttgggcgtct cggccgtcca gagccgcggg
     9781 gcggttacgt tgcgggcgat ccgggcgatc gccgtttctt ttccccgggc cgtcgtcacc
     9841 ggggcgccgg cggagaagac tgtcccgcct tggccgtcaa ggagggtgat ttcgaccgtg
     9901 gagaggccgc cggcccctcc cggccggccg gtcacctcga ccgagacgtc gaggaagccg
     9961 ttccggccgt cggccgtcag gtccgcccga gcccagaaat cgcggatccg gacccggggc
    10021 cgggcgagca gatcgacgtc ccgctcgatc ccgctgatcc gccagaaatc ctggcactcg
    10081 aggtaggagc cgtccgacca gcggaaaacc tgcagggcga cctcgttgtc tccctccttg
    10141 aggaaacggg tgatgttcca ttcggccggc gtcttggcgt cctcgctgta cccggcgtat
    10201 cggccgttga cccagacgta gaaggcggat ttgaccgcgc cgaaatgcag gaagacgtcc
    10261 cggtccttcc agtcggccgg aacgctgaac gttttcttgt aacagccgac gggattgttg
    10321 tcggctggaa gattgggcgg atcgggcgtc tcgccggcct tgagccattc ataatccgtg
    10381 tcgaggtaga tcggcactcc gtatccctgg aattcccagt tggaggggac ggcgatctcc
    10441 ttccagccgc tggcgtcgaa gcccggcttg aagaacccgg ccggaacgtc ggccggcttc
    10501 ggcacccagt ggaatttcca cgggccgttg aggcagcggc ggcgggcgga ggcgtccttg
    10561 tcgttctcga gggccgcggc ttcatcggga tagggaatga agaaagaccg cggcgcttcc
    10621 ttgcccgccc cgacgatccg cggatttttc cattcctcgc cggtcgggcc cttgagatcc
    10681 tgggcggccg cggcggccgc gaaaacggcg gccagggcca gagcgagcgg cgcgagcatc
    10741 acattccgga gcttttccat atctgcctcc atttccttcg ggggcgcggc ctccgccgtt
    10801 agggacggct cgtgcttaac ccggtcattg atcatgccga tgacggctgg aacgggtatc
    10861 gtccggacaa ggcttttttc gaagccggtt cggaaaaacg tcctcttcct ccggcgtcga
    10921 tcggcctcga ccggacgaga attgtttttt ttatttttat tttttgacag gatccgtcgt
    10981 tcccggtttt tctcatgaag aagggtgtca atcgaatgaa ccggtaaatc aattaagagg
    11041 atgagagccg cgtcctcaaa tccgaaacca gggcttcgat ttcgggcttg gccggctcgg
    11101 gcaaggggag aagcggccgg cggaccggtc ccccgtaaaa gccttggatg tccatggcgt
    11161 atttcaagcc cggaaggccg tacgtctcca tggcggcctt gttcagcggg gtcacgtcga
    11221 gctgccgttg ggcggactcg tcgatccggc cttccttgaa gaggcgataa atgtccaagc
    11281 aggcggccgg ggcggcgttg gccacggcca ggatcgcccc ggaggccccc atgaccaggg
    11341 ccggcagaac gacgaatccc gagccgacca ggcaggaaaa atcctccgga acatgaggga
    11401 tgatttttcc caggtaggag aggatgcccg agctttcctt gaggccggcg atgttcgggt
    11461 gggtcgacag ctcgatcaga agctcgggat cgatcgtcac cccggtgttc tgggggatgt
    11521 tgtagacgat caccggagtt ttagccccgt cggccaccgc gagataatgg ccgcgaagga
    11581 cgtcccggct cattttcgcc ttgtaatagc aaggcggccg gacgagggcg gcgtcggccc
    11641 cggccgcggc catccggttg gtgaaggcga tcgtcagctt cgtggattcc tgccccgtgc
    11701 cggcgatcac cagcttgtcc ggcgcggcgg tttcccgggc cgcccggatc agcctctccg
    11761 actcgtcgtt ggagagaagg acgcactcgc ccgtcgaacc gaggacgacg tagcccgaga
    11821 ggtcgaaggc gttgaatttc gcgatgttct cacggaagcg gtggacggcg aggtcttcgc
    11881 cgtcgaacgg ggtggtgagg gcggggaaga tcccttggaa tttcttgctc atcgatgttg
    11941 tcctcgtcgg cgggggattc ggaccggcgg ctttcccgct cggccctccg caaatcggct
    12001 tcatcgaagc cgaaataatg acccacttca tggatgaccg tcgaccggac ctcgtcgcgg
    12061 atttcatctt ccgtcccggc ccagcgctcg agcggcttct ggaagatgag gatgacgtcg
    12121 ggcgggatgt tgccgtaata ggagccgcgc ttgtccaggg ggatcccgtg gtagaggccg
    12181 aaaagctccc agggcgaccg caggcccatg tcctcgacga tctcggcgga gggaaaatcc
    12241 tccaccagca cggccacgtt ctcgattttc tcgcggaagc tccggggcaa gccgtcgatc
    12301 gcttcggcga cgagcttttg gaaccgctcc cgggacaagg tgatcatgat ggggccgatt
    12361 tttctatcat aggggattga gcggcggggg tcaatgagga tcgcgtccgt ctcccggcgg
    12421 gatctccggt cgaaatatta ttccttgtcc gaaattggta taattccgcc atgctggatc
    12481 ctaaattcgt tctcgaaaac gccggtctgg tcatggacaa gtgcgcccag cgcggcgtcc
    12541 ggatcgacct ctcggaattc ctccgcctgt ccgaggccag gaaggagacc ttgcgcgagg
    12601 tcgaggatct gcggtcctgc cggaacaagg cctccgagga agtcgccggc ctcaagcggt
    12661 ccggccggga cgcgggcgcc ctgatcgccg agaccaagct cgtcggagag cggatcaaaa
    12721 ccctcgagga cgggctgaag ccgctggagg agaggatccg gacgaccctc ctcaatatcc
    12781 ccaacctgcc cgacgagtcc gtgcccgtcg gggcggacgc ctcgggcaac gtcgaggtcc
    12841 ggcgggccgg cgagccccgg gagttcgatt tcgagccgct ggcccactgg gatctcggaa
    12901 cgggccttgg gatcctcgat ttcgagcggg cggccaagat cgccgggtcg cgcttcacgg
    12961 tctccctggc cgcggccgcc cggatggaac gggccctgat caatttcatg ctcgacatcc
    13021 acacccggga acggggcttc actgagatcc tcccgccgtt catcgccaac gaagcgagcc
    13081 tgacggggac cggcaacctt ccgaaattcg ccgcggacct cttcaagctg gaggactatc
    13141 cgtggtatct cgtcccgacg gccgaggtcc cgctgacgaa cctccaccgc gacgagatcc
    13201 tggacggagc ggccctgccc gagcggttgg ccgcctacac gccctgtttc cggtccgagg
    13261 cgggctccta cggcaaagac atccgcggcc tggtccgcca gcatcagttc aacaaggtcg
    13321 agatgatggt cttctcgctc ccggaaaatt ccatggacga gctcgaatcc atgaccgcgg
    13381 gggccgagga agtcctcaag cgcctcggcc tgacctaccg ggtcgtagcc ctatgcaccg
    13441 gggacatggg attcgccagc cgcaagactt atgacctcga agtctggatg ccgtcccgcc
    13501 ggggcttcat ggagatttcc tcctgttcca actgcggcga tttccaggcc cgccggtcga
    13561 acatccgctt ccgccgggac cccaaagcca agccggagtt cgtccacacg ttgaacgggt
    13621 ccggactggc cgtcggccgg acggtggccg cgatcctcga gaacttccag ctggacggcg
    13681 gatcgatcga cgtcccccag gccctccgcc cgtatatggg cggcctcacc cggatcgccc
    13741 gatcataaaa cgcccttcct catttccgga acgattcgga tataatgaat tcccgcggaa
    13801 ggatggccga gtggttgaag gcggcggtct tgaaaaccgc tacccggtat ccccgggtcg
    13861 ggggttcgaa tccccctccc tccgttcctg cgcggagggg cgcttacctg ctgtcctccc
    13921 ctagtcttag gggaggcagg aggggtagag acggacaaga aaagtcttaa aagcgccccg
    13981 gtattaaatg cgcggagggg cgcttacctg ctgtcctccc ctagtcttag gggaggcagg
    14041 aggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggtagag gcggaatcgc
    14161 catgaccatt tatacgatcg gattcgccgg gaagccggcc aaccggtttt ccggccatcc
    14221 cctctaaggc cggaaaaccg gaggcaagcg gaaatccccc cggaagggtt tcctttcgct
    14281 ttccgattct ggtaagatac gcccgaatgc aggaaatgac gagagcgcgc cctcgttttt
    14341 ttcttttttg gattgtcttt ttttatgtcc tccttgcctt tccggccgag gccagcctca
    14401 tccgcgtgcc tgaggattat tcgtctatta aaatggcggt gagcgtttca agcgacgggg
    14461 acatcatcga tgtcggcgac ggcttttatt tcgagaagga catcgtcatc gaccgatgcg
    14521 tctccctccg gtcgcggcgg ctgttcggag cggtgatctc cggctccgac aacgaagact
    14581 cgaattccgc cgtgctttgg gtgcggtcgg aatgcgagat ttcgggtttc atcatcaaga
    14641 actgttatca tggaatccgc cagcggggca gcttggacgc ggactggaaa gcgagcgacc
    14701 tgggttttat ggactgcaca tatggaattt caatcgatga tcgtaccggc cgggtcgggt
    14761 cggtcgaagt ccgaaacgtt atttttgatc gaggcctaac ggccctcgcg atcaacgacg
    14821 gccgggccat cgacgcccgg cgcgttttca tctcgcgcgc ccgccaggcc gtatcggcgt
    14881 ttgattacga ccgctgcttt ttagatgaga tcattttatg gaatgttttc tttttctttg
    14941 atccgatatg taaggataac gatctcattc aatcagggcc ttcgattttt aaattccatt
    15001 cttgggaccc gcctctcctc ttgaatgaga tgagccgccg gtttgacggc ttgttcgctc
    15061 gttctcccaa gcccggagcg agggacgagg acaatcgccg gcggaaagcc gtcctggcgg
    15121 tcgtcctggg cgaatcgttt ctcgaggccg gagatccccg cacggcgacc cgccgttttc
    15181 aggaggcggc gatcctgacg agccgggacg aagtggcgga aatccaatgg cgcatcccct
    15241 gggggatggc ccgggcggcg gaagccgcgg gggatggaaa gcgggccgtc ggccattatc
    15301 gcaaggccgt cgccgaggtc gaccggatcg gcctcgggat tccgctgccg gacctgctgg
    15361 ccgatttccg gcgggaaaaa atcgatcttt acgacgcttt catcaagtgc ttggtcgcgg
    15421 ccgaggcgag ggagcccggc ctgggatggg ccgaggaggc ctttgacgtc gccgaacaat
    15481 cgaaagcccg gagcctgcta tcgagcctcc tggcgttcgg agaaggcgtc gagaacgccg
    15541 gccgcgaggc ccgtaaaaag gccgatctcc tggccttccg cttgagccgc tgccagctcg
    15601 agctccagga tcccgacctc ccgccggagc ggaggacggc cctcctggag aagttgaagc
    15661 ggatcgaggc cgagcgacac aacttcttcc tccagctcgg ccggctcctg ccgccggaac
    15721 cgcgcggaac gttcatcgac accctgtctt tggaccaggt caagcacggc cttctcggtc
    15781 ccgccgacgc cctggtcgaa tacatcgtcg gaaagacggc ctcttacgcc ttcctggtga
    15841 ccccgaacgg attcgatgtc gccgttttgc ctccggagtt ccagctgaaa gcgctggtcg
    15901 aaggctacct cgagtttctc acctttcccg cggaaagcga attccggggg tggagcggag
    15961 gccatcggct gtgggaaatt ctgctcggcc cgttcgagga tcgcctgggc tcggtcgacg
    16021 ccttgatcat cagtccggac ggcccgctcc atcacctccc cttcgagacg ctcgtttccg
    16081 acccggtccg gcggcgattc gccgtcgaga gccatgaatt ccgctacggc cactccgcgt
    16141 cttcgctgga gagactccgg cggcgcgcgc ttccttccgc gcggccgatg gattttctcg
    16201 gggtggcggc gacatcccgc atcccggcga tcagcttcga aaccggcaaa cggatcaatt
    16261 tccccgccct gcccaacacg gcccgggaga tccaaaaaat aggaaggctt tttcccccgg
    16321 agaggacgac gattctggcc ggcgccgaag acctggaggc ggctttcaag gcggcctctc
    16381 tcgatgccta cggaatcatc cacttcgccg cccacgggtg gatcgacgac gaggagtgga
    16441 tgcgaagcgc ccttctcctc gggacggacg gctcgggagg cgaggacggt tttctccagc
    16501 cggctgagat ccggcggctg ccgcttcggg cggacctggt cgtcctttcg ggatgcgaga
    16561 cggccggggg acgcttggaa agaggcgagg ggatccgggg cctggcccag gcttttctaa
    16621 aagccggagc ccggtccctg gtcatcagcc aatggtcgat cgaggaccgg gtggcggtcg
    16681 atttcatgga ggctttttac cggggagtgg cgaaaggcta ttcttatgcc tcggccttgc
    16741 ggcgggctaa gctcgcgttc ttggagtcaa cgcgccggca tccgtattat tgggcgcctt
    16801 ttgtctgcct cggcccgtag gatcttattt tccttggtag gcgaagaccg cctcggaccg
    16861 ggcgataaac tttgtcatat catcaaaggc ttctacggtc caaatgtatg tctttccggg
    16921 gacgatcctt gtcgcgagct tttccatgtg gagaacatag agggcggttt tcagggaggt
    16981 cttcggcaca atgtcgttca agctatcgtc gattaagcgg aaccaataat tttccccgcc
    17041 tggaggagtc tcccagcgaa acagaatcgt cttgccgtcg gaaaggacca gcgggcggcg
    17101 cagcgtcatg gcctctcctc cggccgagcg gacgacccgg atcggagcgc gcgaggaaaa
    17161 aacggaatat gtgacggccc cggccgcggc gaaaagaagc gcggccgcgg cccaagcccg
    17221 cgtccggcgc ggaaagcggc caagagcgga cgtctgacgc ccgcgggcga ggcgggcttc
    17281 gcgacggctc cgccggacga gattccggaa atcgcgcttc ctggcccgga cgtcctgtcg
    17341 gatctcgtcc aaaacctcga ttttcgcccg gcattccgga caaacgagga ggtggtcgat
    17401 cagggattcc cgctccccgt ccgaaagctc attccggaag aacttcgcca gcgtctccgg
    17461 actcggacac ggggaccatc cgccgttcga cttcctcttt tcgtgatgcg ctttgttcat
    17521 tgtcttccaa tgctgaggcg aggcagaatt tccgcagctc cttgatgctt cggtataaaa
    17581 gatgccggac cttgggcact gtccaaccca agtgagcggc cgttttctcc agatccattc
    17641 ccgccagatg gcatttaacg accagccggc gcttctggga aagtcttccg accaggatgt
    17701 ccagaagctc ccggcgttcc atttcctcaa aagccggatc cgcggacttc cagcctggaa
    17761 ccggtccgtt cgcgccgccg ccgtggtcat cgagagagac cgcctttaat ttgtccccaa
    17821 taacagccag ggttgtcgta aaaacaacct tccacaaata agactccaaa cggtcgattt
    17881 ttttcccgct tgtgagcatt ttccatattt tgaattggac ttcctgaagg acatcttccc
    17941 ggtcggcgaa ggaaatgtcg ggaaacttcc gggcgacgat ggtccggacg gactggtcga
    18001 tttcggcgat cagccgttcg gtccagattc gtccgccgga tttcatatcg ccgaatgctg
    18061 ccttttccgg atgttcacgg gaatttcatc tgccttcggc cgtttatgtt agctaaatta
    18121 aggattttta tcaattcgtg aaaatcgggg aatctaaaac gcgaagttct tttgtctaac
    18181 acatttataa taatcaatta acatgatcga gagatccccc gcgcatttta gacgcgggaa
    18241 aaaattcctc gatttgcggt cttattaatc ttacgcccgc aatcagcaaa acgggttagg
    18301 gggacttggg tggggggact ctccagccaa taaagagagg agaagatcgt cgcgaagaga
    18361 gaggaaagga gaggaaaaaa gaaggattag tcgtgcaagg gaacccggag gtcgatcgtc
    18421 cggattccct tgaccgcccg gtcggaaaaa aaatctccgg ccgggaaaag cgcgaaaccg
    18481 ccggattcgc cgctgttgtt ttccttagcc atcaataaaa tctcccgaaa gtcatttcgg
    18541 tcggcgatct cggccaggga aaaggccgcc cggtagccgt cggaggcgat gaccgcgacc
    18601 aatccttcct tcatcgcccg atcagccgcg gggtatttgc cggacagaac ttcccggagc
    18661 gcaacccccc ggaatgtttc aatccccaga aaccctttgc cccggccgta gaaaaccgcc
    18721 gggtattcat aagcggcgct tttttccggc agccggctca attccccgat cagaacgccg
    18781 ttgagacaga ggcgcaaatt cgtcttcgat gaggcctccc ccttgccgtc cgcgattccg
    18841 gacatcccca tgtccagcga ccggatcgtg atccgggagg ggcgggagat gtttcggatc
    18901 gagaggagat ccgagccggc gaccagcttg ctttccgagg gcaggggcca gagatccttg
    18961 gtcagcgagg ggacgagccg ggagacggcc gtcgccagga tgatccggtg ccgatcggcc
    19021 ggataataga tctcgcccca ggagaagacg gctttttcac ccgcctcatt cgcgacttcg
    19081 acatacacgt cgatgatgcg ggaatactcc ccggagttct ttttttcgac cgtgacccgg
    19141 tcgaggatgt cggccaggga atagccgtca tagcggtacg cgccgaggaa aacgaggcgg
    19201 ccgtcatccc cccggaccgc ttccttggcc gccatgctcc gcagcggcag ggaggccagg
    19261 acgacggacg tttcgcgccc gacctcgccg ccgacgagga cggcttcggg atggttcaag
    19321 gggaccgccg gcgtgtcgtc gaaaaggtca ttcgtttggg cgcccagggg aagagccgca
    19381 aggagaaccg cgagaaccgg gccggcgatc gctcttttca ttttcattcc tccgccccat
    19441 cctacgttcc gcgaccgcgg tttgcaagcc gcgttttccg gaaagcccga ttccggctat
    19501 actatccgtc cggaggcggg atgagcacgg ctgtcttctt ctcggagggt ggtttttcat
    19561 gtccgcgcct cgccttgccc gggaattggt cccggcccca acctccattg agcgggcgga
    19621 aaggacatcc tcggggagaa cgcgaatgag tcgaaacaat tccgggcggc tgatctccgc
    19681 ggtcgtgtgt ttcctggggg cgttgacggc ttgccgggcc gccggtccgg ccgggcggac
    19741 ggtcgatgtc ggcggccatc ggctgtattg cgagcttcac ggccggggga cgccggccgt
    19801 cgtcatcgac gtcggagcgg gggagtcctt caaggattgg gaggccgttg tcgcggcgct
    19861 ttcgccggag accgcggtcg tcgtttatga ccgggccggg tacggccgaa gccaggcggg
    19921 gccgttgccc cgcgacgcga aagccgaggc cgcggatctc gaggccctgc tccggaaggc
    19981 ggagatcccc gggccgtacg tcctggtggg gcattccctc ggcggcctga acgtccagag
    20041 attcgcccat gactttccgg cggacgtcgc cggccttgtc cttctggatc cgccgccgcg
    20101 cggctggttt gccggggagg cgttccccgg cttgaagagg atgttccggg cggcggccga
    20161 ggatcttgcc gcccaggcgg aggcggccaa aaaaagctcc gacggcgacg agaggatgat
    20221 ggcgtcctat cttgaagcgg tcgcctccga acacgcggcg atgttcggga ccaccgcttc
    20281 gcaggtgagg gaggcggccg cgttcgggga tcttccgttg atcgtcgtgg ccgccggccg
    20341 tcccaatccc gccttcgggg aggaagccgg ggcttttcag gcgtactgga tcgaggagaa
    20401 ccgggcgctg tcccgcttgt cccgaaaagg ggagttcatc ctcgccgaaa acagcggtca
    20461 tcgcctccat cgggacgcgc cggatacggt cctccgggcg attcggaagc tggccggccg
    20521 ataaaagcag aaatcgggga gggaggaaag ggagggtcaa gcctccgccg gaggcttttt
    20581 ccggcggatg accagcgtcg cgcagaccgc ctcggtctcc aggacccggt tctcttccag
    20641 ctgccgccgg acgtagtcca ggaaaagatc ttttttatcc gggacggcgg cggccagctc
    20701 gagacagcgg tttttcagct tcgcgaagat gtcgtcgtcg gattccttga ggtcctcctc
    20761 ggaaatgaca gcttcctcga ccagttccat cccggcctcc tcgatctccc ggagaatgac
    20821 gctttttttc cggatgatgg ggtgagtgga ggaagaagcg tcgtggaaat atccctcgtc
    20881 gacgataaaa aggccttcgg gtttcaggca gggagcgagg gtcgtgagcg tttcccggta
    20941 gtccccgaaa accgggccga tggctcccag gacgatcacg tcgaagccgg ctagtccctt
    21001 gatttcctcc cggatgtccc cgacctcgaa ccggcagcgg gcgtcgacgc cgtgttcggc
    21061 ggcccgggcc cgggcctcgg cgatgaaggc ctcgacggcg tcgatcccca ggcaggagca
    21121 gtcgaaagcg gccgcgaccg ggatcgagac ggcgcccttg ccgcagccca ggtccaggag
    21181 tcggaagccg gccttgcggg gaaggcggcg gcggaggact tggatgatga ggtcgggcga
    21241 agccccgatt tgccagatgt cctggaggat gtaggggagg tagggataga gggaggcgtc
    21301 ggttccgtcc atcgccagga cgacgctttc ctcgaccgtt ctcccgcggg agtcgacgtt
    21361 gatggccatg atacgacgat tatatgaaat cgggcgcggg cggccaacct ctcttcgcgg
    21421 ggccgtcatt cgcccttgat ctttttgagc atcgcctcgg cgttggcgtt tttcggattc
    21481 agggcgaggg atttctcgta attcgcgatc gctttttcct tctggccgtc ggccaggcag
    21541 gcttcggcga gagaatcgta gacgttgtag gaatccgggt aggcgacggc gttcagctcg
    21601 aagatcgcga gggcgtcctt gatttttccg tcggccagga gcttgtagcc gagggtgttc
    21661 agctggtcct cccggaagtt gtattcgccg cccttgtcct ttttcagcca caggtagagc
    21721 gagacggctt ggctgatgcc tttttcccgg atcgcttggt agagcgtggc cgcgatcggg
    21781 gttttcggcg tctccacgga aaggatttcg taatcgaaga tcaggacggc gttcggcgga
    21841 atgatctcgc cggacccgtt ttcgccgtaa ccgagctccg ggggcatggt gaagagaaat
    21901 cggtcgccga cgcgcatcag ggcgacgccc tcggcgcagc ccggaatgac cggatccagg
    21961 cccaggatga aatagaaggg attttccgga tcgcgagaat tccagaaaac ggttccatcg
    22021 ggaaacgagc cgatgccgtg gaggccgatc tccttccccg gttcggtcac ggggccgtcg
    22081 ccgcgcttca ggatccggta tttcaatccg ctgggcgtgg tgaattcctc accgggcggc
    22141 ggcgcggctt gcggggccga agcggcggcc tgggggcggg ccggggggga gagggccgga
    22201 agagccgaag acgaaagacc gaggaccgcg gccggaagaa taagggcgat tcttgagaag
    22261 cagggacgca tcactcaacc ccttcatgat tttttaatgg atgttcttga ttcggcattt
    22321 tcttatggta ctcgcaaaaa gaaggcaagg aaagggggcg gggatttgcg cgggctcgcc
    22381 aagcaagaat gtcccccgtg acggccaagc ggaaaggccc cttgcggcgg gaggcggaca
    22441 cggaggaaag gatgccctta agccgccgcc ggagcggccg tttatatcac ctcgagcttg
    22501 cgcccgacct tctcaaacgc cgcgacggcc cgctccatct gctcccggct gtgagcggcc
    22561 gagatctgaa ccctgatccg ggactggccc ttgggaacga cgggatagac gaatccgacg
    22621 acgtagatcc cctcggcgta gagctcccgg gccatctgct gggccaggcg ggcatcgttg
    22681 tcgaatttgc ggaacatcac cgggacgatc gggtggctgc cgtcgatgat gtcgaagccg
    22741 agggctttca tcgccccgcg gaagaaaacg gtattgtcgc ggagcttttc ccgcagctcc
    22801 tggccctggg cgaggagatc gaaggcggcg atgccggcgg ccgcgatcac cggggggatc
    22861 gtgttggaaa agaggtaggg acgggatttc tgccggagga tttcgatgat ctcccggtgg
    22921 ccggtcacga acccgccggt cgctccgccc atggccttgc ccagggtcga ggtgatgatg
    22981 tcgacttggc cgagcatgcc cgcctcctcg accgagcccc ggccggtcgc cccgaggacg
    23041 cccgtgccgt gggaatcgtc gaccacgacg agcgtgtcgt aggtgttccc gagctcgacg
    23101 atccggcgga tgtcggccac gtcgccgtcc atggagaaga cgccgtcggt gatgatcatg
    23161 atgttgcggg ccgggacggg ctccttgtcc aggcccgggc cgtcccagac gtccatcccc
    23221 tcgcgggctt ttttcagggc tttttcgagc gaggccatgt cggcgtgatc atagatgaag
    23281 cgcttggctt tgcacagccg gatcccgtcg atgatggagg cgtgattgag ggcgtcggtc
    23341 acgaccgcgt cctccacgcc gaggagggtt tcgaacaggc cgccgttggc gtcgaagcag
    23401 gaggaataga ggatcgtgtc ctccgtgtgg aagaattcgg cgattttctg ttcgaggatt
    23461 ttgtgaagat cctgcgttcc gcagataaac cggactgagg ccaggccgaa gccgtgctcg
    23521 tccagggcct tgcgggccgc ctcgaggatt ttcgggtggt tggccaggcc gaggtagttg
    23581 ttggcgcaaa agttcagaac gttccgcgga tcgctcccgg ccggatattc gacccggatc
    23641 tcgggctttt gggcggagag aatggtccgc tcgtccttga agagccccga gctcttgatt
    23701 ttgacgagct cctgggcaaa gtcgtacttg atgcgctggt acatgggaat cctcctcggt
    23761 tccggaccgc cctgaatccg gggccgggac ttgatccggt tccgggagca ggcgggaggc
    23821 ggcggtgtcg gtccgggctc agggcggccg tccgcattca tcataccaca aggcggaaac
    23881 gaggtcgcgg aatccccctc ttccaataaa tccgaacctg tttaaccatt tccctgatta
    23941 attgggaacc aggtcaacca tttccccatt tcccgtccgg ccattcagta cctggttaac
    24001 catttcctga atttaccggt ccttaacgtc cgaaattcgt tataataccg gggcatgcaa
    24061 ttcgagggat ggatcaagcg ggatgcccaa ggcagcgccg gtctccggag gctggggaag
    24121 ggggcgggcc gaacgatttc cgccgtcctg gctcataccg ccgacacaac ggtgcttttc
    24181 cccgcggccg ggctgatctg gctgtttgcg ggcgagcctt ggaaatcgtg ggcttggaaa
    24241 gcggggctgg ccctcttggt cgggacgttg gcgaccggga ttctcaagct cgtcttcaag
    24301 cggcgccggc ccgacgggcg atggggagct tttgaccgcc tggtcgatcc gcattccttt
    24361 ccgtccggcc acgccaaccg gacggcggtc ctggccgtcc tggcggcgtt atatgcgccg
    24421 ccgtctgccg ccgcggccgt cattctatgg gccctggcgg ttggactgtc ccgggttgtc
    24481 ttggggatgc attatctttc tgatgttatc gccggttggc tcgtcggcgc cgccgtcggc
    24541 ttgtttttct agctcaatcg gtacctgttc aaccttttcc ctatttcggg ggtggaaacg
    24601 cagatcagtc cgaaaaccgc cggctcgatg gcgataacag attcattgac aatcagatag
    24661 atccatgtcg aaacccggaa atggttaacc aggttccgat tagaaatggg gatatggtta
    24721 aacaggttcg gattcagcgt ccttgaaaga ggacccaagg attggccttc atgtcccgca
    24781 aataaggaag ctgccaggcg tattcaggat gggcggcggc gaagggcgcg gccagaaatt
    24841 cctccccgca ggggtgcccc atccggaccc agattttcat ctcgccggcc aggccggccg
    24901 aagtgacctt ggcctgaacc gccccggacg gcgagaatcc gccccaatcg gcttccgggg
    24961 cgccggccgg atcgcggtcg atatgcccgc agagggtccg ggcgctcgga cgggcttccc
    25021 ccgagcgcgc gtcgacatga tccccgagaa actccatggc tttctcgacg tcgatcttcc
    25081 ccttgttttc ggccatcagc tgctcccacc ggtcgcgccg gatgtaaacg gactgatccg
    25141 cgtcatccgg attgaaggtc gtctcctcgg cgatcagctt ttcgtccagg gggaagttag
    25201 ccccggcgaa ccatccgtcg gtcgtccgcc aaatccggtg atttttcaag ccgagctcga
    25261 gccgggcgat ttcgttcgtt ttcacgtcgc cgacgagcca atcgttggcg tatcctccgt
    25321 ttccgtccgt cgtcatgatc cggatgaaat cgtcgatcga ggcggcgtat tgggcggccg
    25381 tccgggcgcg ggcgaactcg ggaacgccct tttcgtcgaa acccgcgaat ccggagatcg
    25441 tcgtttccgt atagacgagg ccggcctcgt tgacgacaaa gtcggagccg ctgtgaatga
    25501 acccgggaat ggcgtccatg agcatccgcc ggccggtgtc ggggacgata tcgaggatca
    25561 cgttccaccg ttccccgatg aggtagtcgt cccaggtgtt gtggcccatg acgatccggc
    25621 cgtcggccgt ccagccgccg gtggcgatga aggcgctgca gcggaggggc ggcttcgtcg
    25681 cggcctcccc ggccccgccc tccgctcttt tcatcctgag ggcgggctcg taataccagg
    25741 ccagctcgat gatgccgttg agggcggcga tgtcgaagcg gtcgtatttc aacccgctct
    25801 ttcccgcccg aaggccttcg gcgaggccct cgatttccat ccggatcggg cgctcgagcc
    25861 tcggccagag gaccttttcg gcggccccgc ggtaaaaagc ccagtcgcgc cccgtctctt
    25921 ttttcaagta aaaggcgaac gttttcagga cgtcgtcgat ctcggccgcg agcagccggc
    25981 cgtgctggta gccgatctcc cgggacgccc cctcgaggcg gacgaacgac cagccggccc
    26041 ggtcctcccg gaaagccccc cgcatcacgc ttcgcgtccc gcacgaaacg gccacgatga
    26101 cggcgacaat gatgatgatg accagaacaa ccagcttttt gagactcacg gcttcctccc
    26161 cggcgatcgt tcccgatccg cccgtcgctt cgcgtttatc gttcctcccg gccgggccgg
    26221 gcggcgtttg atgaatcctt gaggcggccg gccttccgga ccgcctcctt cagcagaaat
    26281 tccatctcgg cgttgacgct gcgcagatcg tcggccgccc atttttccag ggcttcgtag
    26341 agatcgccgt cgagccgcag aaggaatttc tttttttccg gcatggatca atcaggtgta
    26401 cagggttccc gtgttgatga tcggcgtcgt ttcgctttcg gaaaccaggg cgaccaggag
    26461 attgttgacc atggctgctt ttttatcctg gtcgagatga acgactccgg cgttttcgag
    26521 gcccttgagg gccatgtcga ccatgccgac ggccccatcg acgatttttt ggcgggcggc
    26581 gatgatcgcc tcggcctgct ggcggcggag catgacttga gcgatctcgg gggcgtaggc
    26641 caagtgcatg agccgggcct cgacgacgtt gacgccggaa accgcgagac gttcctggac
    26701 ctgctcgcgc agcttggccg cgatcttgtc gggatcggtc cggagcgact cctggtcctt
    26761 gtcgtgggag tcgtatgggt agtggctggc cagctgccgg accgccgtct cgctctggat
    26821 ggcgacgaag ttcttgtagt tgtcgacgtt gaatctggcc ttggcggtgt cgatgacgtg
    26881 ccagacgacg acggcggcga tctcgatcgg gttgccgcag gcgtcgttga ctttgatctt
    26941 ctcgctgacc aggttggaga cgcggagcgt cacccggtgc ttggcgcaga acgggttggt
    27001 ccagtgaaag ccggccgtcc ggaccgagcc gaaatagcgg ccgacgaaag tgaagaccat
    27061 ggcctcgttg gggtgaagga cgaagaatcc gccgcaggag aggccgacga gaagcaaggc
    27121 cccgagacgg ccgaaaaagg ccgcggtcat cggcggctcc ccgagaagct tgatgaaata
    27181 gacgacggcg tagacgatca gaatgatgtc gatcgccacg accgcccatc cgctgacgtg
    27241 gaaggctctc ttttctttaa tatcgatcat gggttcctcc ttagatatcg aaatgatatc
    27301 atattgatat ttactttgtc aagggattta aggcgtatta tttttcggga ttttatcgat
    27361 cgagggattg aaaacgcgtc gttttttcgg acgcttttct tcagaccggc tggccgaacc
    27421 ggagcttcgt ctccggttgt gtccgccttc ggccaaatgg tagaataccc ctgcttagca
    27481 gccacggaga ggtgctggag tggctgaaca ggagcgcctg ctaagcgctt gtctcggcta
    27541 aaaccgggac cgcgggttcg aatcccgccc tctccgccag ttttcttccg gttggcgcgg
    27601 ttgatctttc tagacggatt ccatgcattt ccggattctc acg
//
LOCUS       DAOOXH010000023        27518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_1528_length_27518_cov_7.223683, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000023 DAOOXH010000000
VERSION     DAOOXH010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 27518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27518
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1528_length_27518_cov_7.223683"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PKV64_04430"
     CDS             <1..454
                     /locus_tag="PKV64_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HPN16360.1"
                     /translation="LLPLLATTVVVGMLQQIMTASGVRGILSFAVISTPLLLLYILLA
                     VIIPFSEGVLTYGGAVILGIPLIWFLDSIGLHATVVIAGLSFLWALGDALPPTALIGR
                     LSMMVSGYKGSYGSFLTQTWIPWVVITIVSVLMVVFSAKLEFLVRWSM"
     gene            465..620
                     /locus_tag="PKV64_04435"
     CDS             465..620
                     /locus_tag="PKV64_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16361.1"
                     /translation="MNIIMIFYYLITAYLVFILGWNFVREKKSVSHLILTLLVLTPLV
                     LRLLRVK"
     gene            631..1794
                     /locus_tag="PKV64_04440"
     CDS             631..1794
                     /locus_tag="PKV64_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HPN16362.1"
                     /translation="MKNEKILKIALGALFAALLIAGGIPLSKHHRFRTPVVAGPGVTR
                     VLRLSDFLPSLKGTAGDTNVYVLEGREPGGAALFMAGTHPNEPAGIIAAMILIENATV
                     EKGTLYVIPTLNVSASRETRPGDGYPLYFDIPTPWGEKRFRMGNRDASPLDQWPDPDV
                     YIHYPEKQLLSFLDIRNTNRTWPGRPNGLLMERVTYAAMELIRKNKVGLAVDLHGAET
                     MFPVTNCIVAPEKAARIATMVSMTVKAVEGFDNHVEPSPQNFRGLSHREIGDYSDALP
                     FLLEAPIPFLDQPTGPKTVKLMLDGKDPFLLKLAASKKLFVPYDENGWPLDKRVGQHA
                     SVLLELLRQYSKKFPEQAIRVSGFPRYADIVQKGAGAWFHDPAEAVAGSVHEE"
     gene            1811..3526
                     /locus_tag="PKV64_04445"
     CDS             1811..3526
                     /locus_tag="PKV64_04445"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPN16363.1"
                     /translation="MTFKRLRPVIGLLAAAAFAGAGLLVFSEKNDSGLDTTLGPDNEA
                     CTVIMVGKAASTDGSTMTTHTADCGTCDWTFGHFPAADHKPGSTRKIYHISQYKTWKE
                     ATKWPYAMSQGETGFSIPQVPHTYAFHHGMFGYMNENQVAISESTLGTVRKLVNNTPS
                     AKLDLTMLSLLGMERSRTARECIEIMGSLAEKYGYGFNDDGEMFAVSDPDEVWIFEIM
                     PVGPLWSPETGKPGAVWCAQRLPDDHVSVCPNESRIGEVNLEDKENFMGSAHMVSFAV
                     AQKLYDPASGKPFNWKRAYSPAEGGAVSTNGRRSRLWRFLDLVAPSLKLDPNTPNMDL
                     PFSVKPDHKLSVQDVMNLTRDKSYGSIFDPVAGIRGGPFKNPNYYAATRKISVANVEY
                     TSLVQCRSGFPSAIGGILWVAFGAQDTSCYIPLYAGMTALPKSFMVGDHFVFNRSSAR
                     WAFDYVDFHTQVVYNRAIQDVEKVRLEHEGAAIQAVPEIDRKAMELYAKSPAKAAAYL
                     TKTCVDQADRIVAAWWDLGDKLLVKYNHIGLYDTEKRVRDRSVPLYDEVWQKAVKMVD
                     TWMEK"
     gene            3561..4208
                     /locus_tag="PKV64_04450"
     CDS             3561..4208
                     /locus_tag="PKV64_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HPN16364.1"
                     /translation="MKKTLAVLLALAGLGLTASAEGGGFKAALPVFTTSAGQSNDANT
                     VNIVMEEAGVLYDYCDVPTVEMLQAGVSLGGKKSAAGFHVESTTDTALFPAGTPFKTV
                     IVAIGASLKGMGASGLTIDAEESRVKAIVDYCKKNKILLVAVHVGGESTRGPAGSDNE
                     RMIDAVAPAADVIIVTKDSNKDGRFAKIAEKTKAPFIQIDYALGLVDIMKNAFAS"
     gene            4389..5333
                     /locus_tag="PKV64_04455"
     CDS             4389..5333
                     /locus_tag="PKV64_04455"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPN16365.1"
                     /translation="MALAKVKDFLIKHFEYILVAVLLLGISFILVFVPQKTSFLNFYF
                     LPVLAAGVILGKREGVLTAFGSILIVVLTVIVFPEDFLASSGNEVLTLVLNLLPWGGF
                     LILAAFTVGYLNEQKQKQTEDLKTAYIGVLEILSKLLESVDRYTEGHSVRVSTLSMNI
                     AIAMGLPRDEVEDIRAAGLLHDIGKFEITADLIRKAAALSREEREALSKHTDMGARII
                     SRVGPVLRRAVPIIMAHHRFFVERVKSAGNEEIPLGANIVAVADAYDAIITDRPYRKG
                     RPPWQAIGEIRKGAGTQFDPAVVEAFERVSAEMISQER"
     gene            complement(5433..5924)
                     /locus_tag="PKV64_04460"
     CDS             complement(5433..5924)
                     /locus_tag="PKV64_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HPN16366.1"
                     /translation="MEERERRVYEALEALGIPYARHEHPAAFTVAEAVEHWKGIPGAH
                     YKNLFVRNKPGKRHYLIVAGHLKALDLLALSRLLGDSRLSFASDERMTRYLGLHPGEV
                     SPFGLLYDEKHEVIVVLDEDLIRAESVNFHPNVNTATLTIATADFLRFLKSRGNRVIT
                     IRF"
     gene            5984..6175
                     /locus_tag="PKV64_04465"
     CDS             5984..6175
                     /locus_tag="PKV64_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16367.1"
                     /translation="MKFLVWLILLVLCWPLALLALILYPLVWLFLLPFKLVGITVNAV
                     FDLFKAVITLPARILRGSL"
     gene            complement(6186..6710)
                     /locus_tag="PKV64_04470"
     CDS             complement(6186..6710)
                     /locus_tag="PKV64_04470"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HPN16368.1"
                     /translation="MSPVAHSGLGLLGWRLFDRKQTVGTLFLYLLIANAADVDFLLHA
                     VFQDRAVFIHQYYTHNLLFVLAVSLGFMFLLRDGPSRWGLVLTGISHLAADVIVVDTL
                     KPVGIRLFFPIAGAFYNLPWFPYLQRGTWKEMATLRNLGVLGLEFGIFVLPVLLLFPK
                     PLWKRFRSSAFWKV"
     gene            complement(6828..7619)
                     /locus_tag="PKV64_04475"
     CDS             complement(6828..7619)
                     /locus_tag="PKV64_04475"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HPN16369.1"
                     /translation="MNIVVLGFGRMGSWLAGELARTHRVFVFDKNPHQTRGASRVEVL
                     NRPDEIAGLKPGWLINAVDLAGTVEAFREVEELLPPECILGDIASIKTSLPDYYRRCR
                     FRFGSCHPMFGPTFADSASLGEESAVVIRESHPEAAAFFEEFFRSKGVTVRTCSFDEH
                     DRMMAYALTMPFIASLVFAVCLDGPSVPGTTFKRHRDVAGKLLGEDDGLLAEILFNPH
                     SLAQLEKVTRKLEFLKHVIAARDEEEARRIFARLRENLGRGRPPG"
     gene            complement(7619..8692)
                     /locus_tag="PKV64_04480"
     CDS             complement(7619..8692)
                     /locus_tag="PKV64_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HPN16370.1"
                     /translation="MTLAMTLEEIRRKIDEIDFELFKLMEERMEWALRTRRLKPASAD
                     PRREAMVLEKAAGAGRPLLRPEFNREIIERVVSESRALQEKGLSLIGFQGEHGAFSEE
                     AVRFRFPGQVALPHPEFEDVFKGVEDGSLDYGVVPVENTLGGTVTRINELLLQTPLHV
                     VGEIRVRISHCLLALPGTDPKEIKAVYSHPRALAQCQGFLRRNRLEARPFYDTAGAAR
                     MLFEERPPAAAAVAGEMCARLYRLEVIKSGLEDRDTNETRFFVLSREPRTGEGNKCSL
                     AFDFETADKAGSLFSVLRVFAEAGLNLTRIESFPRPADPSGCAFFLDFHGTLGDPAVA
                     GALDRLKTVARFVKIFGCYDEAR"
     gene            complement(8689..9957)
                     /locus_tag="PKV64_04485"
     CDS             complement(8689..9957)
                     /locus_tag="PKV64_04485"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPN16371.1"
                     /translation="MTRLVLNVGGGRSDIRIGVGAFRLEDHALKKRTLILTDRKVQAL
                     HGSLFRGWNTLVLDRGEKMKTLAVVERISRALLRRGGDRGATIVGVGGGVICDLAGFA
                     AAVYMRGIRFGFVPTTLLAQADASLGGKNGVNLDRYKNVIGTFRQPDFVLADPRFLST
                     LPPAAVREGLAEVVKAAAIRDRALFIELENAAERALAGDLAVLERLVARAAAVKIAVV
                     ARDERERGARTLLNFGHTFGHALEKTAGLSHGEAISAGMAAAAGDSLGGIVACAVMGL
                     PVGLGEPFFDSFESVLGHLLFSIPGIKGLEFGAGFRGAALRGRDYNDVILDASGRTRT
                     NHDGGLNGGITNGNDVVFRVAVRPTATLGRPQETVDMITGRPAAVQAGGRHDACFALR
                     MPVILEAAAAFVAADFQLCFHPAFHEGGLP"
     gene            complement(9954..10766)
                     /gene="aroF"
                     /locus_tag="PKV64_04490"
     CDS             complement(9954..10766)
                     /gene="aroF"
                     /locus_tag="PKV64_04490"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPN16372.1"
                     /translation="MQGLKLTARSDKPRTVVEAGPLRLGAGFAVIAGPCSVESELQLL
                     TIARAVKAAGADALRGGAFKPRTSPYAFQGLGLKALKILEKARRETGLPIVTEVLDPR
                     DVSWVSEFVDVLQVGARNMQNFSLLKEVGKGSRPVLLKRGMHSTIEEWLNSAEYILSA
                     GNPRVILCERGIRTFEVYSRNTLDLSAVPAVRELTHLPIIVDPTHGTGRISLIEPMSL
                     AAVAAGSDGLIVEVHHHPEEALSDKAQALLPAAFAALVRRVRAVRSCVEALS"
     gene            10985..11221
                     /locus_tag="PKV64_04495"
     CDS             10985..11221
                     /locus_tag="PKV64_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16373.1"
                     /translation="MTFLEFVMRERAGTTVFVPAILMWRFDRPAGFLITIDAGRRDET
                     GDREDPMIVPRDGKTGGVYFRYWEMNSSTSGMPP"
     gene            complement(11170..12564)
                     /gene="gatD"
                     /locus_tag="PKV64_04500"
     CDS             complement(11170..12564)
                     /gene="gatD"
                     /locus_tag="PKV64_04500"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HPN16374.1"
                     /translation="MNDFNNTEYKGYRGRALEVLQSFKALVWSDAKIVTADGSYIGII
                     LPRSETADPDHVVLKLRSGYNIGVAVDRIQDVEIQGRKEAHYKIPEKEFPFDPRKPRV
                     KLLGTGGTIASRLDYRTGAVIPAFSPGELYGSVPELADICNLETEKIYGVFSENMGPE
                     QYIGTAEAIAREIEKGVQGIVIGHGTDTMHHTAAILSFMVQNSPVPIVMVGSQRSSDR
                     PSSDAALNLMHSVKTAAESDIAEVLVCMFGPTSDIYGLLHRGTRVRKMHSSYRSTFRT
                     IGDIPIAMVSREKITPLRQDYKRRRTDRGVVLNTAFEEKVSIVYYYPNMKPDIIDSLV
                     ENGYRGIVIAGTGLGHVNKPLYPALKRAQDRNVAVYMTVQTLWGFVQMYVYDTGRDMM
                     ELGVVPAANMLPEVAYVKLAWTLGQTDDPAEVKRIMLTPIAGEITDREPSNGYLIFQG
                     GIPEVEEFISQYRK"
     gene            complement(12561..14489)
                     /gene="gatE"
                     /locus_tag="PKV64_04505"
     CDS             complement(12561..14489)
                     /gene="gatE"
                     /locus_tag="PKV64_04505"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HPN16375.1"
                     /translation="MAYDFIFKPFENMSPEDYAAVGFKSGLEIHQQLLTGRKLFCRCP
                     AGRYSDVYHAEILRHMRPTLSELGEYDGTALMEFKTKKEIIYRINRESVCTYEMDDTP
                     PFMINEEALDIALAIAMLYDCVMVDELHIARKQYLDGSIPTGFQRTTIVGVDGRIDFN
                     GRDIRIVQLGLEEDACREVSDIGHRRVYKTDRLGMPLIETVTGPDMRTPQEAAAVAQI
                     LRRIVRSTGRVRTGIGAGREDVNVSVTGGTRVEIKGVPRIPRIPRLTYHEAMRQWNLL
                     RLREELVRRSVTTASFASSIEDVTKPLKRTRYTPIEAAVKAGMIVCGVRLNGFAGLLR
                     WPTQTDTYFSQEISDRVRVIACLTVLPNILHSDSPGESLSSSEWTAVKKALGARDEDA
                     VVVVWGGEADARLGAQEIAARAREATIGVPSETRQALPDDTNGFERILPGPDRMYPDT
                     DLPPKKIGPGRLKAIRASLPPSVQERERWYRRLEVPADVVECLSFSPAARLFETAVKE
                     HAVDPKTAAVILIQYPKRLKRKGLPPLPPDAPAFAEIFEALAEKRLFREGVLPLLEAS
                     LAQGKPVSDLLPAAGRPGDLEKDALRIAETLRGPRFADGPRRMRRAMGLLMAEWRGRI
                     DGAGAARLMADRLGKVSP"
     gene            14780..15613
                     /gene="aroF"
                     /locus_tag="PKV64_04510"
     CDS             14780..15613
                     /gene="aroF"
                     /locus_tag="PKV64_04510"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPN16376.1"
                     /translation="MNSANLYGLARTVGRRTRIPVGPVEVGKDFLLIAGPCAVENETQ
                     TITTAREVKEAGADMLRGGAFKPRSSPYSFQGLGLRGLQILAKAREETGLPVVTEVID
                     PRDAGWVGEYADVLQIGARNMQNFSLLKETGKSGRPVLLKRGFHSTLAEWLNCAEYIL
                     AEGNPRVILCERGIRTFETYTRNTLDLSMVPAVREISHLPVIVDPSHGTGKSSLIPAM
                     SLAATAAGADGLMLEVHYRPEEAVSDRDQTLSTGEFRTLAGKIRSLRSFMLEQGGLDA
                     D"
     gene            15603..16658
                     /gene="aroB"
                     /locus_tag="PKV64_04515"
     CDS             15603..16658
                     /gene="aroB"
                     /locus_tag="PKV64_04515"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPN16377.1"
                     /translation="MPTEILDLEFSPPPRRSRIILGAKIARLPAWLPGSGKVLFVCDP
                     VVESLHGRSLPARFPRITLPRGEKRKTQASVRRLYLSFLDHGLDRSSLVVAVGGGIVC
                     DLAGFAAATFMRGLPFAFVPTTLLAQVDAAIGGKNGSNFLGYKNLIGTTAQPRFVFSS
                     PEFLETLPRREWTCGLAEVIKAAAVADIELFRFLEKNADRLLERDREAVRCAVAAAVR
                     VKTDIVVADELEAGRRRVLNFGHTLGHALERASGIRHGEAVSIGMAAAARLSVRRSGL
                     AERDADRLIRLIERFDLPVRPGRAWPDIRQALLQDKKKAGNRVDLVLLERLGKAVVAG
                     VGREEMERGFHDLCQRG"
     gene            16636..17292
                     /locus_tag="PKV64_04520"
     CDS             16636..17292
                     /locus_tag="PKV64_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HPN16378.1"
                     /translation="MICVSVGKTSFSACLRLLRTVDCAEIRLDLNSFSAVEIRKIFSR
                     PKTLIAACRPGRLSNPIRRERLAEAVSAGASYVDVDGRESPGFRDYIKAEARRAGCRL
                     IFSYHDFRGTPSRSLMERVIDRYVRAGADVVKIACLARTPSDCARLVSLYDRKDERGK
                     SLLAFAMGPAGSWTRVAATLLGAPFTFAAAGKGRETAPGQLDRKSMKAVELLLQGRTS
                     "
     gene            17289..18593
                     /locus_tag="PKV64_04525"
     CDS             17289..18593
                     /locus_tag="PKV64_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPN16379.1"
                     /translation="MKLFGVAGRPILHSLSPEIHRALYARFGVDAAYLRVRADGPEDI
                     LTLMREAGFKGMNATSPFKESLFPLLDRLDDSAVSTGAVNTILAGTGGLRGFNTDTRG
                     IAEALKKSGIPLRRERVLVLGAGGAGRAAVRAMQAENAFVTVINRGKEKTELLAESMG
                     CASLPWEELPAALSGLRLLINTIPASPALFPIDRLRPGTTILDADYRNAPLEAAARDR
                     GCRYIPGTEWLLRQGVAAFLLMNGLRPEEAPLPEEDFLPGLSARVPEKKKGIVLVGFM
                     GSGKTTIGRRLAESLSYAFLDTDEWIEEREQMTVSEIFARRGEARFRALEREAVGRFT
                     GVESLVLSCGGGAVCDPASRAEMKRHGWVVWLYTPLARTAERIKNGSRPLWQDRREDL
                     LRLFTERKTAYALTADLVAFNIKHVQETADDIRSEIDPFLAP"
     gene            18553..19812
                     /gene="aroA"
                     /locus_tag="PKV64_04530"
     CDS             18553..19812
                     /gene="aroA"
                     /locus_tag="PKV64_04530"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPN16380.1"
                     /translation="MTSEAKSIRSSRLEGAVDAPPSKSHMQRAAAAALLAEGTSVLRR
                     PSLCDDAQASLRAVRALGAEVEDSGPDIRIRGGFNPRMPDIDCGEAGLCLRLFSAVAA
                     LSSRAIVLSARGSLRQRPMGMIEAPLRRLGVVVRTDGGFPPLEVRGPLRGGEADVDGS
                     VSSQFLSGLLTALPKAERSSLLQISDLKSRPYVDLTLEVLRGFGVRVDHESYRTFRVE
                     AGQSFRPADIEIEGDWSGASAWLAAAAVDGKISVRGLRRDSRQADVKMLEALDACGAR
                     LSWEGNTLTVEPGRLRSFVFDAADSPDLFPALAALACRCPGTTRIKGASRLAHKESDR
                     ATVLVRELGRIGGDIRRDGDWMEIRGTSLGGGEIDAHGDHRIAMAAAIAALGAREPVR
                     LSGGACVAKSYPLFFQDLRRLGGDIHE"
     gene            19805..20839
                     /locus_tag="PKV64_04535"
     CDS             19805..20839
                     /locus_tag="PKV64_04535"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPN16381.1"
                     /translation="MNSFGRVFRLHLFGESHGPAVGVVLDGCPAGLPLRPEDFRPDLA
                     RRKSGAPGTTARRETDIPEILSGVCRNRTTGAPVVVSFANADTRSGDYARFRRVPRPG
                     QADFAAGVKFGENQDLRGGGHFSGRLTLGLVAAGVAAKKLVRPMRISARLVSAGGEAD
                     AAAAASQAARERNSIGGLVECTAAPVPPGLGEPFFDSLESLLAHILFAVPGIKGVEFG
                     RGFAAASMRGRDFNDPLIASDGKTRTNNAGGINGGISNGNPVCFRVAARPPASIGLRQ
                     ETLDRKTGRTVSLRIGGRHDACFALRLPVIVEAAAAVVFADLLLLEQRIPRVWTNRSG
                     RGRRWAASRP"
     gene            20897..22369
                     /locus_tag="PKV64_04540"
     CDS             20897..22369
                     /locus_tag="PKV64_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HPN16382.1"
                     /translation="MLIPEKKAFLRDAAEYSVVPVSKEIPADFETPLSLFLKSRGLFL
                     LESIERGEHVGRFSIIACGRKCGITVTGRKVEVRGPNGNGRESVTEKSLPDPLEEVRR
                     FFAGLKAPRYEHLPPFWGGGIGYLGYETVGYFENIPAAGADGPIPDACLVVPEMVLVY
                     DSVKRSIFIIASVFPGQDPENDYAAAADRIGAMESLLAGRIPAQPRPVRKKRPVLRLD
                     TDRDEFLRNVLTCQDLIRAGDAYQVVLSRAFSVDTDAHPFAVYRALRMINPSPYLFFL
                     DFGAFALTGSSPEVMVRVQGREMLVKPLAGTRPRGGTVAEDDRLAKELLADAKEKAEH
                     IMLVDLARNDLGRVARPGSVDVVDYMAVERYSHVMHLVSSVKAEMDESRDVFDVIRAV
                     FPAGTLSGAPKIRAMEIISELEGRRRDFYGGMVFNLGFNGNFDSCITIRTILKKGRTA
                     FFRAGAGIVADSVPENEFRETEHKAEALFAAIERAGAEGD"
     gene            22371..23960
                     /locus_tag="PKV64_04545"
     CDS             22371..23960
                     /locus_tag="PKV64_04545"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component
                     II/anthranilate phosphoribosyltransferase"
                     /protein_id="HPN16383.1"
                     /translation="MILLLDNFDSFTYNIFQYVRRLGHEVEVRRNNAVTAEEIDRLRP
                     SHLIISPGPGRPENAGISMEAVRAFQGKIPILGICLGHQAIGAALGGSIVRAAALCHG
                     KESEIYHDGKGIFSGMKNPFRAIRYHSLAVDRSSLPSELDVSAWTEDGEIMGIRHKRW
                     SLDGVQFHPESIGTDRGIEILANFLNPRPRPSLIRAAIRKASAGQDLEMGEAETLMEE
                     IASGNATPAQIAGLLTALAGKGESVSEIGGFARALRRKAAPVRKPEGRPVIDTCGTGG
                     DGSGTFNISTCAAFIAAGAGATVAKHGNRSITSRCGSADLVEALGVNIAAPAEVMEKA
                     LREIGLAFLFAPKFHASMKHAVPVRLDLGIRTIFNILGPLANPAGADRQLIGVYSEDL
                     VPRIAETLARLGTSRALVVHGFDGLDEITLGGLTRAAEIRDGWIRLLDIHPRDFGFEP
                     CRESDLKGGSLKINTEIALRILDGERGPKRNAAVMNAAAVIYLAGLQPGPAEAAAAAE
                     KAIDTGAARRKLDELIAATGY"
     gene            23962..24738
                     /locus_tag="PKV64_04550"
     CDS             23962..24738
                     /locus_tag="PKV64_04550"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HPN16384.1"
                     /translation="METILEILIRETREAERENIRRFDPDRYPVKSRPGPGAWTSLAA
                     RDFFLIAEVKKGSPSRGLIRSELDPAALAGEYEAAGAAAVSVVTESRRFFGRKDFLPR
                     VKARVRLPVLRKDFLIHPCQVYESLALGADFILLIAACLTGGSLAEMRETAGQLGLGV
                     LLEIHSPNELDRALAVHPDFLGINNRDLNDFSVDWRRGLELRPSVPPGIPVIAESGIN
                     GPGQVRAFKEAGFAGILVGEHLVRADDPVRALRELLDGQD"
     gene            24725..25357
                     /locus_tag="PKV64_04555"
     CDS             24725..25357
                     /locus_tag="PKV64_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HPN16385.1"
                     /translation="MAKIKICGLTRREDLVLAAEMGADFAGFVFVPDSARYIRPEDAA
                     RELNPEGMPESLERVGVFVNEQPARIREVFRLCRLNIVQLHGEESPDYCRSLGLPYWK
                     ALRPETASGLDPAAAYPGAVILLDGRAPGVRGGAGVRVSQDILSAASKLGRRIMIAGG
                     ISEENLAEILAYRPYGLDLSSSLEDRPGIKNPARMRRFFGLLKGNNHEPS"
     gene            25344..26582
                     /gene="trpB"
                     /locus_tag="PKV64_04560"
     CDS             25344..26582
                     /gene="trpB"
                     /locus_tag="PKV64_04560"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPN16386.1"
                     /translation="MSHHERRRYFGPYGGRFVPEMLVPALAELEEAFFHFRRDAEFRN
                     ELDGLLRDYAGRPTPLYFAPNLSRAGGGCRIYLKMEGLAHTGAHKINNVLGQSLLARK
                     MGKARLVAETGAGQHGLAVAAAAARFGLKARIFMGKTDMDRQFPNVFFMRRMGAEVIS
                     VLDGGRTLKDAVNAALKFWIENQADTHYLLGSALGPFPYPLMVRHFQSVIGREVKRQI
                     LKAEKRVPDVLVACVGGGSNSLGLFHPFLDRREVRCIGVEAGGRGSEPGEHAVRFGPN
                     GRIGIVQGYKSVFLQDDDGQVLPTHSLSAGLDYAGISPELADLRRRGRVEFVSVRDGE
                     ALEGFELLARLEGIIPALESAHAAAYGLRLARTLPPERLMVINLSGRGDKDLFITARA
                     LEKEPWIDFLSREAGHVPTA"
     gene            26566..27354
                     /gene="trpA"
                     /locus_tag="PKV64_04565"
     CDS             26566..27354
                     /gene="trpA"
                     /locus_tag="PKV64_04565"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HPN16387.1"
                     /translation="MSQPLDDLFKASRSTLLMAHLVAGDPDPDGSLDLALVLARNGAD
                     LLEIQIPFSDPLADGPAVMAANRRALERGARPEDAFRLMERIREKILIPMTVMSYGNI
                     PFRMGFERFASRCGESGAGAAIIPDLPWDERLPGLRKAFGRRGIHLVRPVSPGMSPGR
                     LEAAVGEASGFLYATLRVGTTGASARIEDRGLDFIRRVKTASRLPAVAGFGVSSPEQA
                     ALLKGMVEAVVIGSRLIEVRSIGGLESVGAFIRRCRAALDEFRT"
BASE COUNT         5003 a         8966 c         8557 g         4992 t
ORIGIN      
        1 gctgctgccc ctgctggcga ccacggtcgt cgtgggcatg ctccagcaga tcatgaccgc
       61 ctccggagtg cgcgggattc tctcgttcgc ggtgatcagc acgccgctcc tcctgctcta
      121 catcctcctg gccgtcatca tccccttctc cgagggcgtc ctgacctacg gcggggcggt
      181 gatcctgggc attcccctga tctggttcct ggactccatc ggcctgcacg ccacggtcgt
      241 catcgccggt ctgagcttcc tctgggccct gggcgacgcg ctccccccga cggctttgat
      301 cggacgcctg agcatgatgg tttcgggcta caagggctcg tacggatcgt tcctgacgca
      361 gacctggatc ccctgggtgg tgatcaccat cgtcagcgtc ctgatggtcg tcttcagcgc
      421 caagctcgaa ttcctagtcc gctggagcat gtgaggaggc cgccatgaac atcatcatga
      481 ttttttatta cctcatcacc gcttacctcg tcttcatcct gggctggaat ttcgtccggg
      541 agaaaaaaag cgtttcccat ctcatcctca ccctgcttgt cttgacgccg ctcgttctcc
      601 gccttctccg ggtgaaataa gggtcccgcc atgaaaaacg aaaaaatcct caagatcgcc
      661 ctgggcgccc tttttgccgc cctcctcatc gccggcggaa tccctctctc caagcaccac
      721 cggttccgca cgccggtcgt ggccggcccc ggggtcaccc gggtcctccg cctgagcgac
      781 ttcctccctt cgctcaaggg aaccgccggc gacacgaacg tctacgtcct cgagggccgg
      841 gagccggggg gagcggccct tttcatggcc ggaacccatc ccaacgagcc ggccggcatc
      901 atcgccgcga tgatcctcat cgaaaacgcg accgtcgaga aagggacgct gtatgtcatc
      961 ccgacgctca acgtcagcgc cagccgcgaa acccggcccg gcgacgggta tcccctgtat
     1021 ttcgacatcc ccacgccctg gggggaaaag cggttccgga tgggcaaccg cgacgcctcc
     1081 ccgctcgacc agtggcccga tccggacgtg tacatccact accccgaaaa gcagctcttg
     1141 tcgttcctgg acatccggaa caccaaccgg acctggcccg gacggcccaa cggcctcctc
     1201 atggaacggg tgacgtacgc ggccatggaa ttgatccgga agaacaaggt cggcctggcg
     1261 gtcgacctcc acggcgccga aaccatgttc ccagtcacca actgcatcgt cgccccggaa
     1321 aaagcggcca ggatcgcgac gatggtctcg atgacggtca aggccgtgga ggggttcgac
     1381 aaccacgtcg agccgtcgcc gcagaatttc cgcgggctgt cccaccggga gatcggcgat
     1441 tacagcgacg ccctgccgtt cctgctcgag gccccgattc cttttctcga ccagccgacc
     1501 gggcccaaga ccgtcaagct catgctcgac ggcaaggatc ccttcctctt gaagctggcc
     1561 gcgagcaaaa agctgttcgt cccttatgat gaaaacggct ggccgctgga caagcgggtc
     1621 ggtcagcacg cgtccgtcct cctggaactc ctccgccagt attccaagaa attccccgag
     1681 caagcgatcc gggtgagcgg cttcccccgc tacgccgata tcgttcaaaa aggcgccgga
     1741 gcctggtttc acgacccggc cgaagccgtc gccggatccg tccacgaaga ataatcaagg
     1801 aaggagcgtc atgacgttta aaagactccg gccggtcatc ggcctgctgg ccgccgccgc
     1861 tttcgccggc gccggcctcc tcgttttctc cgaaaagaac gactccggtc tcgacacgac
     1921 cctcggcccc gacaacgagg cctgcacggt catcatggtc gggaaagccg cctcgacgga
     1981 cggttcgacc atgaccaccc atacggccga ttgcggcacg tgcgactgga ccttcggtca
     2041 ttttccggcg gccgaccata agcccggctc gacccggaag atctaccaca tcagccaata
     2101 caagacctgg aaggaagcga cgaagtggcc gtatgccatg agccaggggg agacgggttt
     2161 ctccattccc caggttcccc acacctacgc tttccaccac ggcatgttcg gctatatgaa
     2221 cgaaaaccaa gtcgccatct ccgagtcgac cctggggacg gtccgcaagc tggttaacaa
     2281 caccccctcg gccaagctcg acctgacgat gctttcgctc ttggggatgg agcgctcccg
     2341 gacggcccgg gaatgcatcg aaatcatggg ttccctggcc gagaaatacg gctacgggtt
     2401 caacgacgac ggcgaaatgt tcgccgtctc cgatccggat gaagtctgga tcttcgaaat
     2461 catgccggtc ggcccgctgt ggtctccgga aacgggcaag cccggggcgg tctggtgcgc
     2521 ccagcgcctg cccgacgacc acgtctcagt ctgtcccaac gaatcgcgca tcggcgaggt
     2581 caacctcgag gacaaggaaa atttcatggg ctcggcccac atggtctctt tcgccgtggc
     2641 ccagaagctc tacgatccgg cctcggggaa gccgttcaac tggaagcggg cctactcccc
     2701 ggccgagggc ggcgcggtga gcacgaacgg acgccggtcc cggctctggc ggttcctcga
     2761 cctcgtcgcc ccgtccctca agctcgatcc caacacgccc aacatggacc tgccgttttc
     2821 ggtcaaaccc gatcacaagc tctccgtcca ggatgtcatg aatctgaccc gggacaagag
     2881 ctacggctcg atcttcgacc cggtggccgg catccgcggc ggcccgttca agaatcccaa
     2941 ttattatgcc gccacccgga aaatctcggt ggccaacgtc gaatacacga gcctggtcca
     3001 gtgccgaagc ggatttccgt cggccatcgg agggatcctc tgggtggcct tcggggccca
     3061 ggacacgtcc tgctacatcc ccctgtacgc cgggatgacg gccctcccca agtcgttcat
     3121 ggtcggcgat catttcgtct tcaaccggag ctccgcccgc tgggctttcg actacgtcga
     3181 ttttcacacc caggtggttt acaaccgggc gatccaggac gtggaaaagg tccggctcga
     3241 gcatgagggc gcggccatcc aggccgtccc ggagatcgac cggaaagcca tggagctcta
     3301 cgccaagagc ccggcgaaag cggccgctta cctgaccaaa acctgcgtgg accaagccga
     3361 ccggatcgtc gccgcctggt gggacctcgg ggacaagctc ctggtcaagt acaaccacat
     3421 cggcctgtac gacacggaga agagggtccg ggaccggtcc gttcccctct acgacgaggt
     3481 ctggcagaag gccgtgaaaa tggtcgacac gtggatggaa aaataagata atgaatcatc
     3541 acgcaagaag gagaaccgcc atgaaaaaaa ctctcgccgt ccttctcgcc ctggcggggc
     3601 tgggcctgac ggcctccgcc gagggcggag gcttcaaagc cgccttgccc gtgttcacca
     3661 cgtcggccgg gcaaagcaac gacgccaata ccgtcaatat cgtcatggag gaagcgggcg
     3721 tcctgtatga ttactgcgac gtcccgaccg tcgagatgct ccaggccggc gtcagcctgg
     3781 gcggaaagaa atccgccgcc ggcttccacg tcgagtcgac gaccgacacg gccctctttc
     3841 ccgcggggac gccgttcaag acggtcatcg tcgccatcgg cgccagcctc aagggcatgg
     3901 gcgcttccgg cctgaccatc gacgccgagg aaagccgggt caaggcgatc gtcgattact
     3961 gcaagaaaaa caagatcctc ctggtcgccg tccacgtcgg cggtgaatcc acccgcggcc
     4021 cggccggcag cgacaacgag cggatgatcg acgccgtcgc cccggcggcc gacgtcatca
     4081 tcgtcaccaa ggattcgaac aaggacggcc gcttcgccaa gatcgccgag aaaaccaagg
     4141 ctcccttcat tcagatcgat tacgccctgg gtctcgtcga catcatgaag aacgctttcg
     4201 cgtcctgacc cggagcaaac gaccgggacg cccgggccgc gcggacacgc ggtccgggcg
     4261 tttttttttg ccttgtgccc cccccttgtg gtatccttcg cccggaaaga tgacgtccgg
     4321 gaccccaaag gtttaacgtt cgcaaggccg gggcgtcgtt cggccgaaaa cggcggggag
     4381 gggggaacat ggcgctcgcc aaagtcaagg atttcctcat caagcatttt gaatacatcc
     4441 tggtggccgt tttgctcctg gggatcagct tcatcctcgt tttcgtcccc cagaagacga
     4501 gctttcttaa tttctacttc ctcccggtcc ttgcggccgg cgtgattctg ggaaaacggg
     4561 agggcgttct cacggcgttc ggctcgattc tgatcgtcgt cctgacggtg atcgttttcc
     4621 ccgaggattt cctcgcatcg tccggaaacg aggtcctcac cctcgtttta aaccttctgc
     4681 cctggggcgg atttctcatc ctggccgcgt tcaccgtggg atatctcaac gagcaaaaac
     4741 aaaaacagac ggaggatttg aagaccgcct atatcggcgt tctcgaaatc ctgtcaaagc
     4801 tgctcgaatc cgtggatcgc tacacggagg ggcattcggt ccgcgtctcg acgctgtcca
     4861 tgaacatcgc catcgccatg ggcctcccac gcgatgaagt ggaggacatc cgggcggccg
     4921 ggctgctcca tgacatcggg aaattcgaga tcaccgccga cctgatccgc aaggcggccg
     4981 ccctgagccg ggaggagcgc gaggcgctgt ccaagcacac cgacatgggc gcccgaatca
     5041 tctcccgggt cgggccggtg ctccggcggg ccgtgccgat catcatggcc catcaccgtt
     5101 tctttgtcga gcgagtcaag tcggccggga acgaggaaat tcccctcggg gcgaatatcg
     5161 tcgccgtggc cgacgcctac gacgccatca tcaccgaccg tccctaccgg aagggccgtc
     5221 cgccctggca ggccatcggg gaaatccgca agggcgcggg gacgcaattt gatcccgccg
     5281 tcgtcgaggc cttcgaacga gtcagcgcgg aaatgatatc ccaggagcgc tgaccggccg
     5341 cgaaaacgct tctcctccga acggcggcac gtcccggacg cctccccctt ttccgaaaat
     5401 cccggcgttg tccgaaaaga acttcggccg gctcagaagc ggatcgtgat gacccggttg
     5461 ccccgggatt tgaggaacct taaaaaatcg gcggtcgcga tggtcagggt ggccgtgttg
     5521 acgttggggt ggaaattgac ggattccgcc cggatcaaat cctcgtccag gacgacgatg
     5581 acctcgtgtt tttcgtcata gagaagcccg aacggcgaga cctctccggg gtggagtccg
     5641 aggtagcggg tcatccgctc gtcggaggcg aagctcagac ggctgtcgcc caggagccgg
     5701 gacaaggcta agaggtcgag cgctttgaga tgcccggcca cgatgaggta atggcgcttg
     5761 cccggcttgt tccggacgaa caagttcttg taatgcgccc cggggattcc cttccaatgc
     5821 tccaccgcct cggccaccgt gaaagccgcc gggtgctcgt ggcgggcata ggggattccc
     5881 aaagcctcca aggcctcgta gacgcggcgt tcacgttctt ccatgattcc gtgaactttt
     5941 ccccgcccgt ctgcgtattt gtctaaggga ggaggtttga cccatgaaat ttcttgtctg
     6001 gctgattctg ctcgttttat gctggccgct ggccttgctt gctctcattc tttaccctct
     6061 tgtctggctg ttccttcttc ccttcaagct ggtcggcatc acggtcaacg cggtgttcga
     6121 cctcttcaag gccgtcatca cccttcccgc ccggattctc cgcggctccc tctgatccgt
     6181 ccatcctaga ctttccaaaa agccgacgac ctgaacctct tccagagggg tttggggaag
     6241 aggagaagaa ccggtaaaac gaagatgccg aactcaaggc ccaacacgcc caagttccgg
     6301 agcgtggcca tttccttcca ggtcccgcgc tgcagatagg ggaaccaggg caagttgtaa
     6361 aaagcgccgg cgatcgggaa gaacaagcgg atcccgacgg gcttcagcgt gtcgacaacg
     6421 atgacgtcgg ccgccaggtg ggaaatcccg gtcaagacga gaccccagcg gctcggaccg
     6481 tcccgaagca ggaacatgaa gcccagggaa acggccaaaa cgaacagcag gttgtgggtg
     6541 tagtactggt ggatgaagac ggcccggtcc tggaaaaccg cgtggagaag gaagtcgacg
     6601 tcggccgcgt tggcgatcag aaggtaaagg aagagcgttc cgacggtctg cttccggtcg
     6661 aacagccgcc agccgagaag accgaggccg gaatgggcga ccgggctcaa gagttcccct
     6721 ccgtctcgat ttcggccggg cggggattcg tcggacagct catggcgtaa ggagctattg
     6781 tacgggattg ccgaagtgaa aaaaagagcg ccggccgccg ctccggatca gcccggcggc
     6841 cgtcctcggc cgaggttttc cctcaaccgg gcgaaaatcc gccgggcttc ctcctcgtcg
     6901 cgggccgcaa tgacatgctt aaggaattcc agcttccggg tgactttttc cagctgggcc
     6961 agagaatgtg gattgaagag gatttcggca agcaggccgt catcctcgcc caggagcttc
     7021 ccggccacgt cccgatgtct cttgaacgtc gtccccggca ccgacgggcc gtccaaacaa
     7081 acggcaaaca ccagggaggc gatgaacggc atggtcaggg cgtaggccat cattcgatca
     7141 tgctcatcga aggagcaggt ccggacagta acgcctttgc ttcggaaaaa ttcctcgaaa
     7201 aaagcggcgg cctcgggatg ggattcgcgg atgacgaccg cgctttcctc gcctaaagaa
     7261 gccgagtccg cgaatgtcgg gccgaacatc ggatggcagg atccgaaccg gaaccggcat
     7321 cggcggtaat agtccggtag cgatgtcttg atggaagcga tatctcccag gatgcattcc
     7381 ggcggcagca actcctcgac ctcccggaag gcctcgaccg tccccgccag gtcgacggca
     7441 ttgatgagcc agccgggttt aagcccggcg atttcgtccg gccgattgag gacctcgacg
     7501 cgggacgctc cccgcgtttg atgaggattc ttgtcgaaga caaacacacg atgagtccgg
     7561 gccagctcgc cggccagcca cgatcccatc cttccgaagc ccaagacgac gatgttcatc
     7621 agcgcgcctc atcataacaa ccgaagatct tgacaaaccg ggctacggtt ttgagccggt
     7681 ccagggctcc ggcgacggcc ggatcgccca aggtcccgtg aaaatccagg aaaaacgcgc
     7741 agccggacgg atcggccggc cgggggaatg attcgatacg agtcaggttg aggccggcct
     7801 cggcgaagac gcgaaggacg ctgaaaagcg atccggcctt gtcggccgtc tcgaaatcga
     7861 aagcgagcga gcacttgttc ccctccccgg tccgcggctc ccggctgagg acgaagaagc
     7921 gggtttcgtt cgtgtcccgg tcctcgaggc cggacttgat gacctccaag cggtacaggc
     7981 gggcgcacat ttccccggcg acggcggccg ccgccggcgg tctttcctcg aacagcatcc
     8041 gggccgcccc ggccgtgtca taaaaaggcc gggcttccaa gcggttgcgg cgcagaaatc
     8101 cttgacattg ggccagggcc cggggatggg agtagacggc cttgatctcc ttgggatcgg
     8161 tcccgggaag ggccaggaga caatggctga tccggacgcg gatctcgccg acgacatgga
     8221 ggggtgtttg gagcagcagc tcgttgatcc gggtcacggt cccgcccagg gtgttttcca
     8281 ccgggacgac gccgtagtcg agactcccgt cctcgactcc cttgaagacg tcctcaaatt
     8341 cgggatgcgg caaggcgact tgtccgggga aacggaaccg gacggcttcc tcgctgaacg
     8401 ccccgtgctc gccctgaaag ccgatcaatg acagcccctt ttcctgaagg gcgcggcttt
     8461 ccgatacgac ccgctcgatg atttcccggt tgaattcggg gcggagaaga ggccggccgg
     8521 cgccggcggc cttctccagg accatcgctt cccgccgggg atcggctgag gcgggcttca
     8581 agcgccgggt tctcagggcc cactccatgc gctcttccat cagcttgaaa agctcaaaat
     8641 cgatctcatc gattttcctc cgaatttctt cgagagtcat ggcgagagtc atgggagacc
     8701 tccttcatgg aatgcggggt ggaaacaaag ctggaaatcg gcggcgacga acgcggccgc
     8761 cgcctcaagg atcaccggca tccgcagggc gaagcaggcg tcgtgacggc cgccggcttg
     8821 gacggcggcc ggccggcccg tgatcatatc gacggtctcc tggggccggc cgagcgtggc
     8881 cgtcggccga acggcgactc gaaacaccac atcgttgccg ttggtgatcc ccccgttgag
     8941 gccgccgtcg tggttggtcc gggtccggcc ggaggcgtca aggatcacgt cattgtaatc
     9001 tcggccgcgc aaggccgctc cccggaatcc ggcgccgaat tcgaggccct tgatcccggg
     9061 gatggagaac agaagatgcc cgaggacgga ctcgaacgaa tcgaaaaacg gctcgcccag
     9121 gccgaccggc aaacccatta cggcacaggc gacgattccg cccagcgaat cgcccgccgc
     9181 ggcggccatg cccgccgaga tggcctcccc atggctgaga ccggccgttt tttccaaagc
     9241 atgaccgaaa gtatgcccga aatttaaaag ggtccgggct ccccgctcgc gttcgtctcg
     9301 ggcgacgacc gcgattttga ccgcggccgc ccgggcgacc agccgctcca ggacggcgag
     9361 gtcgccggcc aaagcccgtt cggccgcgtt ctcgagctcg atgaacaagg cccggtcgcg
     9421 gatcgccgcg gccttgacca cttcggccag tccctcgcgg acggccgcgg gcggaagcgt
     9481 ggacagaaac cgcggatcgg ccagaacgaa atccggctgg cggaacgtgc cgatgacgtt
     9541 tttgtaacgg tcgaggttca ccccgttttt tccgcctagg gacgcgtcgg cctgggccag
     9601 cagagtcgtg gggacgaaac cgaaccggat tccccgcatg tacacagccg cggcgaaacc
     9661 ggccaggtcg cagatgacgc ccccgcccac tccaacgatc gtcgcgcccc ggtcgccgcc
     9721 ccgccgaaga agcgcccggg aaatccgctc aacgacggcc agggtcttca tcttttcgcc
     9781 tcggtccaga acaagtgtgt tccagccgcg gaacagggac ccatgcaagg cctgcacttt
     9841 gcggtcggtg agaatcaacg tccttttttt gagggcgtga tcttccagcc tgaaagcccc
     9901 gacgccgata cggatatccg agcggccgcc cccgacgttc aaaaccagcc gggtcatgac
     9961 agggcctcga cacaggaccg gacggcccgc acgcggcgga ccagggccgc gaaagcggcc
    10021 ggaagcagcg cctgggcttt gtcgctcagg gcttcctccg gatgatggtg gacttcgacg
    10081 atcagcccgt cggacccggc cgcgaccgcg gccaggctca tgggctcgat caagctgatc
    10141 cgtccggttc cgtgggtcgg atcgacgatg atcggcagat gggtcaactc ccggacagcc
    10201 ggaacggcgc ttagatcgag ggtgttgcgg gaataaactt cgaacgtccg gatcccgcgc
    10261 tcgcagagga tcacgcgggg attgcccgcg ctcaggatgt attcggcgct gttgagccat
    10321 tcctcgatcg tcgagtgcat tcccctcttt aacaagaccg gccgggagcc cttcccgact
    10381 tctttcagca gggagaagtt ttgcatgttt cgggcgccga cctggagcac atccacaaac
    10441 tccgagaccc aggaaacgtc ccggggatcg aggacctcgg tgacaatcgg aagccccgtc
    10501 tcgcggcggg ctttttccag gattttgagg gccttaaggc ccaagccctg gaaagcgtac
    10561 ggcgatgttc gaggcttaaa ggccccgccg cggagagcgt cggccccggc ggccttcacc
    10621 gcccgggcaa tcgtcagcag ttggagctcg ctttccacgc tgcacggccc ggcgatgacg
    10681 gcaaatccgg ccccgaggcg gagggggccg gcctcgacga ccgtccgagg cttatcggag
    10741 cgagcggtta atttcaatcc ttgcatggga acaccttttt ctcgatggac aatccggtcc
    10801 gggaaaagaa aggggttggg ggtatggggc ttaattcggg cttatcgccc gaaatagaac
    10861 ccgtaataga aagaccgggc ggcggcggcg gaagaaaccc cggtcgtaag catggcgcca
    10921 gtatagggat tcgccgtttt catgtcaagg aaaatcggcc gcatttcccc ctgccttgat
    10981 tttcatgaca tttctcgagt tcgtcatgag ggagagggcc gggacaacgg tcttcgtccc
    11041 cgcgatcctg atgtggcggt tcgatcgtcc ggccgggttc ttgatcacga tcgacgccgg
    11101 acggcgggat gagaccggag accgggagga tccgatgatc gtcccgcggg acggaaaaac
    11161 agggggcgtt tatttccggt actgggagat gaattcctcg acttccggga tgccgccctg
    11221 aaagatgaga tagccgttag agggctcccg gtcggtgatc tccccggcga tgggggtcag
    11281 catgatccgc ttgacctcgg ccggatcgtc cgtctggccg agggtccaag cgagcttgac
    11341 gtaagcgacc tcgggaagca tgttggccgc cggaaccacg cccagctcca tcatgtcccg
    11401 gcccgtgtcg tacacgtaca tctggacaaa gccccatagc gtctggacgg tcatgtagac
    11461 ggcgacgttg cggtcttggg ccctcttcag ggcgggatag agcggcttgt tgacgtgccc
    11521 cagccccgtt ccggcgatga cgatcccccg gtagccgttt tcgaccaggg agtcgatgat
    11581 gtccggcttc atgttggggt agtaatagac gatcgagacc ttttcctcga aagcggtgtt
    11641 gaggacgacg ccgcggtccg tccggcggcg cttgtaatcc tgccgcaggg gcgtgatctt
    11701 ctcccggctg accatggcga tggggatgtc tccgatcgtc cggaacgtcg accggtagct
    11761 ggagtgcatt tttcgaaccc gcgttccgcg gtggaggagg ccgtagatgt ccgacgtcgg
    11821 gccgaacatg cagaccaaaa cctcggcgat gtcgctctcg gccgccgtct tgacgctgtg
    11881 catcagattc agggcggcgt cggaagacgg ccggtcggac gaacgctggg atccgaccat
    11941 gacgatcggc accggcgaat tctggaccat gaaggagaga atggccgcgg tgtgatgcat
    12001 cgtgtcggtc ccgtggccga taacgatgcc ctggacgccc ttttcgattt cccgggcgat
    12061 ggcctcggcc gttccgatgt actgttccgg ccccatgttt tcgctgaaga cgccgtagat
    12121 tttctcggtt tcgaggttgc agatgtcggc cagttcgggg acggaaccgt agagctcgcc
    12181 cggcgaaaaa gccgggatga ccgccccggt ccggtaatcg agacggctgg cgatcgttcc
    12241 ccccgtcccc aggagcttca cccggggctt ccggggatca aagggaaact ccttttccgg
    12301 gatcttgtaa tgggcttctt tgcggccctg gatctcgacg tcctggatgc ggtcgaccgc
    12361 gaccccgatg ttgtagcccg agcggagctt caggacgacg tggtcgggat cggccgtctc
    12421 cgagcggggc aggatgatgc cgatatacga cccgtcggcc gtgacgattt tcgcgtcgct
    12481 ccagacgagg gctttgaagc tttggaggac ctcgagggcc cggccccggt agcccttata
    12541 ctcggtgttg ttgaagtcgt tcatgggctg acctttccca gccggtcggc catcaagcgg
    12601 gcggccccgg ctccatcgat ccggccgcgc cactcggcca tcaagagccc catcgcccgc
    12661 cgcatccggc gcggtccgtc ggcgaaacgg gggccccgga gggtttcggc gatccggagg
    12721 gcgtctttct ccaagtcgcc cggacggccg gcggccggca gcaggtctga aacgggcttt
    12781 ccttgggcca gggaggcttc caggagcggc agcacgccct cccggaaaag gcgtttttcg
    12841 gccagggcct cgaaaatttc cgcgaacgcc ggcgcatccg gcggcaaggg aggcaagccc
    12901 ttgcgcttca ggcgcttggg atactggatg agaatcacgg ccgcggtttt cggatcgacc
    12961 gcgtgttcct tgaccgcggt ttcgaacaac cgggcggccg gcgaaaagga aagacattcg
    13021 acgacatcag ccggcacctc gagccgccga taccaccgct cccgctcctg gacggagggc
    13081 ggaagcgagg cccggatcgc cttgagccgc cccggaccga tctttttagg cggaaggtcg
    13141 gtgtccgggt acatccggtc gggcccggga aggatgcgct cgaaaccgtt cgtgtcgtcg
    13201 ggaagggcct ggcgcgtctc ggaaggcacg ccgatcgtcg cctcccgggc ccgggcagcg
    13261 atttcctggg ccccgaggcg ggcgtcggcc tcccctcccc agaccacgac cacggcgtcc
    13321 tcgtcccggg ccccgagcgc cttcttcacc gccgtccact ccgagctgga cagcgattcc
    13381 ccggggctgt cggagtggag gatgttggga agcaccgtca agcaggcgat gacccggacc
    13441 cggtcggaga tttcctggct gaaatatgta tccgtctgcg tcggccagcg gagaaggccg
    13501 gcgaagccgt tgaggcggac tccacaaacg atcatcccgg ccttgaccgc ggcctcgatc
    13561 ggcgtatagc gcgtccgctt gagcggcttg gtcacgtcct cgatggaaga ggcgaaggaa
    13621 gccgtcgtca cgctccgccg gacgagctcc tcccgcagcc ggagaaggtt ccattggcgc
    13681 atggcctcat ggtaagtcaa gcggggaatc cggggaatcc gggggactcc cttgatctcg
    13741 acccgcgtcc cgccggttac gctgacgttg acgtcctccc ggcccgcccc gatccccgtc
    13801 cggacgcggc cggtggaccg gacgatccgg cggagaatct gggcgaccgc ggccgcttcc
    13861 tggggagtcc gcatgtcggg gccggtgacc gtttcgatca ggggcatccc caggcggtcc
    13921 gttttgtaga cccggcggtg gccgatgtcg ctgacctccc ggcaggcgtc ctcctcgagt
    13981 cccagctgga cgatccggat gtcccggccg ttgaaatcga tccgcccgtc cacgccgacg
    14041 atcgtcgtcc gctgaaagcc cgtcgggatg ctcccgtcca ggtattgttt ccgggcgatg
    14101 tggagctcgt cgaccatgac gcagtcgtag agcatggcga tcgccagggc gatgtccaag
    14161 gcctcctcgt tgatcatgaa cggcggcgtg tcgtccatct cgtaggtgca gacgctctcc
    14221 cggttgatcc ggtagatgat ttctttcttg gtcttgaatt ccatcaaggc cgtgccgtcg
    14281 tactcgccga gctcggagag ggtggggcgc atgtggcgga ggatttcggc gtgatagacg
    14341 tcgctatagc ggccggcggg gcagcggcag aacagcttcc ggccggtcag aagctgctgg
    14401 tggatctcca gccccgactt gaatccgacc gcggcgtaat cctcgggcga catgttttcg
    14461 aacggcttga agatgaaatc ataagccatg acgggcgggt cctccggccc ggaggggaca
    14521 ttttcctcgt tggccccgtc caagtcaaga aaaagcgccg gcccgctcga tttgacaacc
    14581 ccggccggac catgataaaa agccgccatg attttgacgg cggaaccgga ctcgatatgc
    14641 gcccgtgcgc cgccgctttc tctttcttct atttctgggc ggtccccgcc gcctaaagac
    14701 tcacaccccc acccacccct tttttcaatc cgggccgcat tccgttttgc ccccggagaa
    14761 atcccgatga ggaggaccca tgaacagcgc gaatctttac ggcctggccc ggaccgtcgg
    14821 ccgaagaacc cgcattccgg tcgggccggt cgaggtcggg aaggatttcc tcctgatcgc
    14881 cggaccctgc gcggtcgaaa acgagactca gacgatcacg acggctcggg aggtgaagga
    14941 agccggggcc gacatgctcc ggggcggggc gttcaagcct agaagctccc cttactcctt
    15001 ccagggattg ggcctccggg gactgcaaat cctggccaag gccagggagg aaacgggatt
    15061 gcccgtcgtc accgaggtga tcgatcctcg ggacgccggc tgggtcggcg aatacgccga
    15121 cgtcctccag atcggagccc gcaacatgca gaatttctcc ctcctcaagg aaacggggaa
    15181 aagcggccgc ccggtccttc tcaagcgcgg gttccattcc accttggccg aatggctgaa
    15241 ttgcgccgag tacatcctgg ccgaaggaaa tccccgggtc atcctttgcg agcgggggat
    15301 ccggaccttc gaaacctaca cccgaaacac cctggacttg agcatggttc ccgccgtccg
    15361 ggaaatctcc catcttccgg tgattgtcga cccgtcccac ggcacgggaa aatccagcct
    15421 cattccggcc atgagcctgg cggcgacggc cgccggggcc gacggtctga tgctcgaagt
    15481 ccattatcgg cccgaagaag cggtcagcga cagggaccag acgctttcga cgggagagtt
    15541 ccggacgctg gccgggaaaa tccggtccct gaggagcttc atgttagaac aaggaggact
    15601 cgatgccgac tgagatcctt gaccttgagt tttctccccc gccccgccga agccggatca
    15661 tcctgggagc gaaaatcgcc cgactccccg catggcttcc cggttcaggg aaagtccttt
    15721 tcgtctgcga tcccgtcgtc gagtcgctcc acggccgaag tctcccggct cgattccccc
    15781 ggatcaccct tcctcgagga gaaaagcgga aaacccaggc ctcggtccgg cgcctctacc
    15841 tgtccttcct cgatcacggc ctggaccgct cctcgctggt cgtggccgtc ggcggcggga
    15901 tcgtctgcga tctggcggga ttcgccgcgg cgacgttcat gcgcggcctt ccctttgcct
    15961 tcgttcccac gactctcttg gcccaggtcg acgccgcgat cggcggcaag aacggctcca
    16021 attttctcgg atataaaaac ctgatcggca cgacggccca gccgcggttc gtcttttcct
    16081 cccccgaatt tctcgagacg ctcccccgcc gggaatggac atgcggcctg gcggaggtga
    16141 tcaaggccgc cgccgtcgcc gatatcgaat tgttcaggtt cctggagaaa aacgccgacc
    16201 gccttctcga aagggatcgc gaagccgtga gatgcgccgt cgccgcggcc gtccgggtga
    16261 aaacggacat cgtcgtcgca gacgagctgg aagcgggccg ccggcgggtg ctcaatttcg
    16321 gccacaccct cggccatgcc ctggagcggg cgtccggaat ccgccacggc gaagccgtct
    16381 ccatcggcat ggccgccgcg gcccggcttt cggtgcgccg gagcggattg gccgaaaggg
    16441 acgccgaccg gctgatccgg ctcatcgaac ggttcgacct ccccgttcgt ccgggccgcg
    16501 cctggccgga catccgccaa gcccttctcc aggacaagaa aaaggcaggg aaccgggtcg
    16561 atcttgtctt gctggaacgc ttgggaaaag cagtcgtcgc cggcgtcggt cgggaggaaa
    16621 tggagagagg attccatgat ctgtgtcagc gtgggtaaaa cgtctttctc cgcttgcctc
    16681 cgtctcttgc gaacggtcga ctgcgctgag atccgcctcg acctgaattc cttttccgcg
    16741 gtggaaatcc gaaaaatttt ttcccggccg aaaacgctga tcgccgcctg ccgtcccgga
    16801 cggctttcga atcctatccg cagggaacgg ctcgcggagg ccgtctctgc cggagcctca
    16861 tatgtcgatg tcgacggacg ggaatccccc ggattccggg attacatcaa agctgaagcc
    16921 cgccgggccg ggtgccggct gatcttttcc tatcatgatt ttcgaggaac gccctcccga
    16981 agcctgatgg aacgggtcat cgaccggtac gtccgggccg gggccgacgt cgtgaaaatc
    17041 gcctgcctgg cccgaacgcc ctcagactgc gcccggctcg tctctctcta cgaccggaag
    17101 gacgaaaggg ggaaatctct gctcgccttc gccatggggc ccgcgggaag ctggacccgg
    17161 gtcgccgcaa cgcttttagg cgcgcccttc accttcgccg cggctgggaa aggccgggaa
    17221 accgctccgg gccaactcga cagaaagtcc atgaaagccg tcgagctcct tcttcagggg
    17281 aggacatcat gaagctcttc ggcgtggccg ggcgtcccat ccttcacagc ttgagcccgg
    17341 aaattcaccg ggcgctttac gcccgctttg gcgtcgacgc cgcttatctc agggtcaggg
    17401 ccgacgggcc ggaagacatt ctgacgctca tgcgggaagc aggattcaag gggatgaacg
    17461 ccacctcccc gttcaaggaa tcgctgtttc cattgctgga ccggttggac gactcggcgg
    17521 tttcgaccgg cgccgtcaac accatcctgg ccgggaccgg cggtctccgc gggttcaata
    17581 ccgatacccg gggaatcgcc gaagccctga aaaaatccgg aatccccctt cgccgcgaac
    17641 gagttctcgt cctgggcgcc ggaggagccg gccgggccgc ggtccgggcg atgcaagcgg
    17701 aaaacgcctt cgtcacggtc atcaatcgag ggaaggaaaa aacggaatta ctggccgaat
    17761 ccatgggatg cgcctcgctt ccctgggagg agctgccggc ggccctgtcc ggccttcgcc
    17821 tgctcatcaa tacgatcccc gcctctccgg cgctttttcc gatcgatcgg ctccggccgg
    17881 gaacgacgat cctcgacgcc gattaccgga acgcgcccct ggaagccgcc gcccgcgacc
    17941 ggggatgccg ctacatcccg ggaacggaat ggcttctccg tcaaggagtc gcggctttcc
    18001 ttctgatgaa cggcctccgg ccggaagagg cgcccctgcc cgaagaggat tttcttcccg
    18061 gtctttcggc ccgcgtcccg gagaaaaaga aagggatcgt cctggtcggg ttcatgggtt
    18121 ccggaaaaac gacaatcggc cggcgcctgg ccgaaagcct gagctacgct tttctcgaca
    18181 ccgacgagtg gatcgaggag cgggaacaaa tgaccgtgtc cgaaattttc gcccgccggg
    18241 gagaagcccg cttccgggcc ctggaacggg aagccgtcgg ccggttcacc ggcgtcgaat
    18301 cgctggtttt gtcctgcggc ggcggcgcgg tttgcgatcc cgcctcccgg gcggagatga
    18361 aacgtcacgg ttgggtggtc tggctctata cgccgctcgc ccggacggcc gagcgaataa
    18421 aaaacggaag caggcctctc tggcaagatc gccgggagga cctcctccgg ctgtttaccg
    18481 aaagaaaaac cgcctacgcc ctgacggccg accttgtcgc tttcaacatc aaacatgtcc
    18541 aggagaccgc cgatgacatc cgaagcgaaa tcgatccgtt cctcgcgcct tgaaggggcc
    18601 gtggacgctc cgccctcgaa aagccacatg cagagggccg cggccgccgc gctcctggcc
    18661 gaggggacat ccgtccttcg ccgtccgagc ctttgtgacg acgcgcaagc atccctccgg
    18721 gcggtccggg ccctgggcgc cgaggtcgaa gattccgggc cggacatccg aatccggggc
    18781 ggtttcaatc cccggatgcc ggacatcgat tgcggcgaag ccggtctttg cctccgcctg
    18841 ttttcggccg tcgccgcgct ttcgagccgg gccatcgtgc tttcggcccg ggggtcgctg
    18901 cgccaaagac cgatgggcat gatcgaagct cccctgcgcc ggctcggggt cgtcgtccgg
    18961 accgacggag gatttccgcc gctcgaggtt cggggaccgc ttcgcggcgg cgaggcggac
    19021 gtggacggct cggtcagctc ccaattcctc tccgggcttc tgaccgccct gccaaaagcc
    19081 gaaaggtcaa gcctgcttca gatttccgat ttgaaaagcc ggccttacgt cgacctgacc
    19141 ctcgaggtcc tccggggatt cggcgtccgc gtcgatcacg aatcctaccg gactttccgg
    19201 gtcgaagccg gccaatcctt ccgcccggct gatatcgaaa tcgaagggga ctggagcggg
    19261 gcttcggcct ggctcgcggc cgccgcggtc gacgggaaaa tctccgtccg cggcctccgc
    19321 cgcgattccc gccaagccga tgtcaaaatg ctcgaagcgc tggacgcttg cggagcccgg
    19381 ctttcctggg aggggaacac cttgaccgtg gagccgggca ggctccggag ctttgtcttc
    19441 gacgccgccg attcgcccga tttgttcccg gccctggccg ccctggcctg ccgttgcccg
    19501 ggtacgaccc ggatcaaggg cgcgtcccgg ttggcccata aggaaagcga ccgggccaca
    19561 gtccttgtcc gggagctcgg ccggatcggc ggagacatcc ggcgcgacgg agactggatg
    19621 gaaatccgcg gaacgagcct cggcggcggc gaaatcgacg cccatggaga ccaccggatc
    19681 gccatggccg ccgccatcgc cgcgctcggc gcacgcgagc cggtccgcct atcgggcggc
    19741 gcctgcgtcg caaaatccta tcccctgttt tttcaagact tgagacgact cggaggagac
    19801 atccatgaat agcttcggtc gcgttttccg ccttcatctt ttcggcgagt cccacggacc
    19861 cgccgtcggc gtcgtcctcg acggctgtcc ggccggtctt ccccttcgtc cggaggattt
    19921 ccggccggat ctcgcgcgcc ggaaaagcgg cgcccctggc accaccgccc gccgcgaaac
    19981 cgacatcccc gagatcctga gcggcgtctg ccggaacagg acgacgggcg ctccggtcgt
    20041 cgtcagtttc gccaacgcgg atacccgttc cggcgattat gcccgcttcc gccgggttcc
    20101 ccgtcccggg caggccgatt ttgccgccgg ggtcaaattc ggcgaaaacc aggatcttcg
    20161 cggcggaggt catttttccg gacggttgac tctcggactt gtcgcggccg gcgtcgcggc
    20221 caagaagctc gttcggccga tgcggatttc cgcccgactg gtctcggccg gaggagaggc
    20281 ggacgccgcc gcggccgcgt cccaggccgc ccgggagcgc aattcgatcg gcggactggt
    20341 cgaatgcacg gctgcccccg tcccgccggg gctgggagag ccgtttttcg actcgctgga
    20401 atcgctgctg gcccatatcc tgttcgccgt tccgggaatc aagggagtcg aattcggccg
    20461 cggattcgcc gccgcctcga tgcgcgggag ggatttcaac gaccccttga tcgcctccga
    20521 cggcaagacc cggaccaaca acgccggcgg aatcaacggc gggatcagca acggcaaccc
    20581 ggtctgcttc cgcgtggcgg cccggccgcc cgcgtccatc ggcctccggc aggaaaccct
    20641 cgaccggaaa accggccgga cggtctccct gcgcatcggc ggccggcatg acgcctgctt
    20701 cgccttgagg ctcccggtca tcgtcgaggc ggccgcggcc gttgttttcg ccgacctgct
    20761 cctcctggaa cagcggatcc cccgggtctg gacgaaccgc tccggccggg ggcgtcgatg
    20821 ggcggcttcc cgcccataaa tatttttccg ctttcttctt tccggttatt ttttccgatt
    20881 ccaaaggagg acccttatgc tcattcccga aaaaaaagca tttttgcggg acgccgcgga
    20941 atattccgtc gttcccgtca gcaaggaaat cccggccgat ttcgaaaccc ctctctccct
    21001 gtttctcaaa agccggggcc tgtttctgct cgaatcgatc gagcgcggag agcacgtcgg
    21061 acgcttttcc atcatcgcct gcggtcgaaa atgcgggatc accgtcaccg ggcggaaggt
    21121 cgaggtccgc ggaccgaacg gcaacgggag ggaatccgtg acggaaaaat ccctgcccga
    21181 ccccctggag gaagtgcggc gtttttttgc cgggttgaaa gccccccgct atgaacatct
    21241 cccccctttt tggggcggag gaatcgggta tctcggttat gaaaccgtcg gatacttcga
    21301 aaatatccca gcggcggggg cagacgggcc gatcccggac gcctgcctgg tcgttcccga
    21361 aatggtcctg gtctacgatt cggtgaagcg ttcgatcttc atcatcgcct cggtttttcc
    21421 gggacaagac ccggaaaatg attatgccgc cgccgcggac cgcatcgggg ccatggaaag
    21481 tcttctggcc ggtcggattc cggcccagcc gcggcccgtc cggaagaaga ggccggtcct
    21541 ccggctggat accgacaggg acgagttcct ccgcaacgtc ctgacctgcc aagacctgat
    21601 ccgggccggc gacgcctacc aggtcgttct ctcccgcgcg ttttccgtag acacggacgc
    21661 gcatcccttc gccgtttacc gggcgctccg gatgatcaat ccctccccct atctcttttt
    21721 cctcgatttc ggcgccttcg ccctgaccgg ctcatctccc gaggtcatgg tcagggtgca
    21781 aggccgggaa atgctggtca agcccttggc cggaaccagg cctcggggcg ggacggtggc
    21841 cgaggacgat cgactcgcca aggagctcct ggccgacgcc aaggaaaagg ccgaacatat
    21901 catgctcgtc gacctggcca gaaacgacct cgggcgggtc gcccgtcccg gcagcgtcga
    21961 cgtggtcgat tacatggccg tcgagcgcta ctcccatgtc atgcacctcg tctcttccgt
    22021 gaaagcggag atggacgagt cccgggacgt cttcgatgtc atccgggctg tttttccagc
    22081 cggaaccctg agcggcgcgc ccaagatccg ggcgatggag atcatctccg agctcgaagg
    22141 ccgccgccgc gacttctacg gaggcatggt cttcaacctg ggtttcaacg ggaacttcga
    22201 ttcatgcatc accatccgga ccatcctcaa aaaaggacgg acggcgttct ttcgggccgg
    22261 ggccggcatc gtcgccgatt cggtacccga gaacgaattc cgggagacgg aacacaaggc
    22321 cgaggccctg tttgccgcga tcgaacgggc cggggccgag ggagactgac atgatccttc
    22381 ttctcgacaa cttcgattcg ttcacctaca acatctttca atacgtccgc aggctggggc
    22441 atgaggtcga ggtccgccgg aacaacgccg tcacggccga ggagatcgac cgcctccggc
    22501 ccagccacct gatcatctcg cccgggcccg gccggcccga aaacgccggg atttcgatgg
    22561 aggccgtccg ggcgttccag gggaaaattc cgatcctcgg gatctgcctc ggccaccagg
    22621 cgatcggagc cgctctcggc gggtccatcg tccgggcggc ggcgctctgc cacggcaagg
    22681 agagcgaaat ctatcatgac ggcaagggga tcttcagcgg gatgaaaaat ccgtttcggg
    22741 cgatccgcta tcattccctg gccgtcgacc ggagctcgct cccctccgag ctcgatgttt
    22801 cggcttggac cgaggacggg gaaatcatgg gcatccgcca caagcgctgg agcctcgacg
    22861 gcgtccagtt tcatcccgaa tccatcggca cggacagggg aatcgaaatc ctggccaatt
    22921 tcctcaatcc ccggccccgt ccgtccctga tccgggcggc catccgcaag gcgtccgccg
    22981 gccaagatct ggagatggga gaggccgaaa cgttgatgga ggaaatcgcc tccggaaatg
    23041 cgactcccgc ccaaatcgcc ggccttttga ccgccctggc cggcaaggga gaatcggtct
    23101 ccgagatcgg cggattcgcc cgcgccctgc gccggaaagc cgcgcccgtc cggaaacccg
    23161 aaggccgccc ggtaatcgat acctgcggaa cgggaggcga cgggagcggg acgttcaaca
    23221 tctccacttg cgccgctttt atcgccgccg gggcgggcgc gaccgtggcc aagcacggaa
    23281 accgctccat cacctcccgg tgcgggtcgg ccgaccttgt cgaagccctg ggcgtcaata
    23341 tcgccgcccc ggccgaggtc atggaaaaag cccttcgcga aatcggactg gcctttctct
    23401 tcgcgcccaa gttccatgcc tcgatgaagc acgccgttcc ggtcaggctc gacctgggca
    23461 tccggacgat tttcaacatc cttgggccgt tggccaatcc ggccggggcc gaccgccagc
    23521 tcatcggcgt ctactccgag gacctcgttc cccggatcgc cgaaaccctg gcccgtctcg
    23581 gaacgtccag agccttggtt gtccacgggt tcgacgggct ggacgaaatc acgctcggcg
    23641 gtttgacgcg ggcggcggaa atccgggacg gttggatccg cctcctggat atccaccccc
    23701 gggatttcgg attcgaaccc tgccgggaaa gcgatctcaa gggaggaagc ctcaaaatca
    23761 acaccgagat cgccctccgc atcctcgacg gggagagggg accgaaacgg aacgcggccg
    23821 tgatgaacgc cgccgcggtc atttaccttg ccggccttca acccggcccg gccgaggccg
    23881 ccgccgcggc cgaaaaagcg atcgacaccg gcgccgcccg gcggaagctc gatgagttga
    23941 tcgccgccac ggggtactga cgtggaaacc atcctcgaaa tcctcatccg ggaaacgcgg
    24001 gaggcggagc gggagaatat ccggcgattc gatcccgatc gttatccggt taaaagccgg
    24061 cccgggccgg gcgcctggac ctccctggcc gcccgggatt ttttcctgat cgccgaggtg
    24121 aaaaagggct ctccctcccg ggggctcatc cgctccgagc tcgatccggc cgcgctggcc
    24181 ggggagtacg aggcggccgg ggccgcggcc gtgtccgtgg tgaccgaatc ccgccgtttc
    24241 ttcggccgga aggattttct gccccgggtt aaggcccgcg tccggctccc ggtcctgcgc
    24301 aaggattttc tcatccatcc ctgccaggtg tatgaatccc tcgccctggg ggccgatttc
    24361 atcctgctca tcgccgcctg ccttaccggc ggctcgcttg cggagatgcg ggaaacggcg
    24421 ggacagcttg gattgggcgt cttgctggaa atccacagcc cgaacgagct ggaccgggcc
    24481 ctggccgttc atcccgattt cctcgggatc aacaaccgcg acctgaacga tttttccgtg
    24541 gactggcgcc ggggcctcga gctacgtccc tccgtgccgc cgggaattcc ggtgatcgcc
    24601 gaatccggga tcaacggacc cggacaggtt cgcgctttca aggaggccgg cttcgccggg
    24661 atcctggtgg gcgagcattt ggtccgggcg gacgatcccg tccgggccct gcgggagctt
    24721 ctcgatggcc aagattaaaa tttgcggctt gacccggaga gaagacctcg tcctggcggc
    24781 tgagatgggg gcggatttcg ccggcttcgt cttcgttccg gattcggccc gttatatccg
    24841 gccggaggat gccgcccggg aattgaatcc cgagggaatg cccgagagcc tcgagcgagt
    24901 cggagtattc gtcaatgaac aacccgcacg gatccgggag gtcttccgac tctgccggct
    24961 taatatcgtc cagctgcacg gcgaggaatc gccggattat tgccggtccc tcggcctgcc
    25021 gtattggaaa gcccttcggc cggaaaccgc ctccggcctg gatcctgcgg ccgcctaccc
    25081 gggggcggtc atcctgttgg acgggcgcgc ccccggcgtc cggggtggcg cgggagtccg
    25141 cgtttcccaa gatattctct ccgccgcctc gaagctcggg cggcggatca tgatcgccgg
    25201 cggaatctcg gaggaaaacc tggccgaaat cctcgcgtat cggccttacg gtctcgacct
    25261 cagcagctcg ctcgaagacc gcccggggat caagaatccg gcccggatga ggcggttctt
    25321 cggacttttg aaaggaaaca accatgagcc atcatgaacg gcgccgatat ttcggcccct
    25381 atggaggacg attcgttccg gaaatgctcg tcccggctct ggcggagctc gaagaagcct
    25441 tcttccattt ccgccgcgac gcggaattcc gaaacgagct cgacggcctt cttcgcgatt
    25501 acgccgggcg gccgaccccc ctctatttcg ccccgaacct gagccgggcg ggcggcggat
    25561 gccgcatcta tctgaaaatg gagggtctgg cccacaccgg ggctcacaag atcaataacg
    25621 tcctcggcca gtctctcctg gcccgaaaaa tgggaaaagc gcgcctcgtc gccgagacgg
    25681 gcgccggcca gcacggactg gccgtcgcgg ccgcggccgc ccggttcggc ctgaaagccc
    25741 ggatcttcat gggcaaaacc gacatggacc ggcaattccc caacgtgttc ttcatgcgcc
    25801 ggatgggggc cgaagtgatt tccgtcctcg acggcggccg gactctgaag gatgcggtga
    25861 acgcggcctt gaagttttgg attgaaaacc aggccgatac ccattacctg ctcgggtccg
    25921 ccctgggccc cttcccctat ccgttgatgg tccggcactt ccaatcggtg atcggccggg
    25981 aagtgaagcg gcagatcctg aaggcggaaa agagggtacc ggatgttttg gtggcttgcg
    26041 tcgggggagg atccaactcg ctcggcctct tccacccttt tctggatcgg agagaggttc
    26101 gatgcatcgg ggttgaggcg ggcggacggg gatcggaacc cggagaacac gcggtccgct
    26161 tcggaccgaa cggccgaatc ggcatcgtcc agggttataa atccgtcttc cttcaggacg
    26221 acgacggcca ggtcctgccc acccattccc tgagcgccgg cctcgattat gccggaatca
    26281 gccccgagct ggccgacctt cgccgacgcg gccgggtcga attcgtctcg gtccgggacg
    26341 gcgaagccct ggaaggattc gagcttctgg cccggctcga gggcataatc cccgccctgg
    26401 aatcggccca tgccgcggcc tacggcctcc gcctggcccg aacccttccc cccgaaaggc
    26461 tgatggtgat caacctgtcc ggccgcggcg ataaagacct gttcatcacc gcccgggcat
    26521 tggaaaaaga accctggatc gattttctct cccgcgaggc cggccatgtc ccaaccgctt
    26581 gacgacctat tcaaagcatc ccgttccact ctcctgatgg ctcatctcgt ggccggagac
    26641 ccggacccgg acggcagtct cgacttggcc ctcgtcctgg ctcgaaacgg ggccgacctg
    26701 cttgaaatcc aaattccctt ctccgatccc ctggccgacg gcccggccgt catggccgcc
    26761 aatcgccggg cgctcgagcg aggcgcccgg ccggaggatg cgttccgctt gatggaacga
    26821 atccgggaaa aaattctgat tccgatgacg gtcatgtctt acgggaacat cccgttccgc
    26881 atgggcttcg aaaggtttgc ctcccgctgc ggcgaatccg gcgcgggagc ggcgatcatc
    26941 cccgacctac cctgggacga gcggcttccg ggattgagaa aagcgttcgg acgccggggc
    27001 atccacctcg tccgtccggt ctcacccggc atgagccccg gccggctcga ggccgcggtc
    27061 ggtgaagcct cgggatttct ctacgcaacc cttcgcgtcg ggacgacggg cgcctccgcc
    27121 cggatcgaag accggggact ggacttcatc cgccgggtga aaacggcctc acgcctgccg
    27181 gcagttgccg gattcggcgt ttcctcgccc gagcaagcgg cgcttctgaa agggatggtc
    27241 gaagccgtcg tcatcggcag ccggctgatc gaggtccgaa gcatcggcgg cctcgagtcc
    27301 gtgggcgcgt tcatccgtcg gtgccgggcg gcactggacg agttcaggac ttgacagtcc
    27361 tcctgcgcta aattatccta tttcggaggg aaagagggga tttcatgacg agacagggaa
    27421 tatcgtcggg gcttgtgata aaaggaaatt cctatcattt catcgacgaa acacagcaag
    27481 accaggaatt ccttggcatg ctttttgcac ctcaaaaa
//
LOCUS       DAOOXH010000024        27427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_1535_length_27427_cov_8.065724, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000024 DAOOXH010000000
VERSION     DAOOXH010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 27427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27427
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1535_length_27427_cov_8.065724"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PKV64_04570"
     CDS             <1..557
                     /locus_tag="PKV64_04570"
                     /inference="COORDINATES: protein motif:HMM:NF036831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPN16388.1"
                     /translation="VDGGTYPLPDPFMIIATQNPIEYHGTFPLPEAQLDRFLLRVKIG
                     YPSAENEKEIIRGRDLYHLAARLEPVLNPAEVLALQDQVDGIRVDESLLGYIVSLASE
                     TRKTKGIKLGVSPRGSLLLRRAAQAHALTYGRDHILPDDVKRVAAPVLTHRIVIESQS
                     FGLLRIHESDTIIQGVLERVPIPL"
     gene            571..3561
                     /locus_tag="PKV64_04575"
     CDS             571..3561
                     /locus_tag="PKV64_04575"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HPN16389.1"
                     /translation="MLTRLGVRFLAAAVLALVGAATSGANLLYLIDGMLWSILAVSWW
                     WSGRNLRGIGARAVFPEQMFVDTEFPLAVFLRKEIGGRSHFLKLRSPSREASLALLKR
                     GKESAVTIPAAFPRRGLNEIGDIWLESDYPFGLFRRKRRVTGLVGLVFPPVFEIYGRR
                     ASPAVRGEDVSIPKRGAGGEFFGLREYDEGEDARLINWKLTAKTGKPLVKEYAEQVGN
                     RITITVADAEGPDAERQISEAASLAKYFIDAGSDVRLRTPEATVAFGHGLIHLGLIFK
                     TLALLGRGKIVERFEKPLPKTKSPAFPGEDPLSPFAYSTVLTAFASLFLIEELNPLVL
                     ASYALLFPLAWLGDRKKRYLLPRPACDALAALYLLFFLFVDLPRAGSLGAVTRLVLFI
                     LGYLLFNPKTGRAPAQLLTASFLVFFLASGQALSLWYFAFFLAFFLSAGAWLIDRRDP
                     RPRPRERTWGRSLLAVTGIAVILAAAAFVVLPRPYSARMRQLLAVTGLTRFQQSLRTF
                     SGMSEQVELGYLGPIHKNSSRVMRVMPGLPDGSPVPEFIHVRGAAFDFFDGRRWRKSP
                     VSFTSEISGRLVSSRRTMAWIRRERGWISAPGYDPQKPFREETYVLSPFLNTSLVFFA
                     GPPGAIEANYQGIFFDFTDTAYFPGAYLEGISYRVRTQGREPSFSRSIRDYGGLLQSH
                     FLQQPGPNERLRRLAVQFAGRAGDARSRAAAVEARLRGGYSYSLAADLGRQDIDGFLF
                     ESKAGNCEYFATAMCLLLRHLDIPSRLAVGFLGSEWNEYGKFFDIRQSDAHAWVEAYL
                     PESGWTTFDPTPPDFTESGAGSLFSRLWSALNDKFEDLQFRWYRYVVGYDTFTQRNFL
                     YNIVRKASEAAVPLLFGLAGFSAALFLLLRRKSRGTLPAWKPSRKKPEDFYETLLVRL
                     GRAGFPRRPSQTAAEYAGETGSAHPELGGLTGLARRHYELRYAGRVLEESERAEIART
                     SNDIAAAARRLKRRRRRDIKGGNA"
     gene            3558..3974
                     /locus_tag="PKV64_04580"
     CDS             3558..3974
                     /locus_tag="PKV64_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPN16390.1"
                     /translation="MKSYRRELFFEIPNRRGFVNITPQVESCLRESGIREGLCLVNSM
                     HITSSVFINDDESGLHHDFEVWLEKLAPHEPVSSYRHNGFEDNADAHLKRQVMGREAV
                     VAVTSGKLDFGTWEQIFYGEFDGRRRKRVLVKIIGE"
     gene            complement(4129..5835)
                     /locus_tag="PKV64_04585"
     CDS             complement(4129..5835)
                     /locus_tag="PKV64_04585"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HPN16391.1"
                     /translation="MSREIDRREFLKSVGTAAAAAALGGAGLILQGCAAGKDYDLVIK
                     GGLVYDGLGGDPFEADIGVSGERIKTVGPIPGRSGRIVVEAAGRIVTPGFIDVHDHSD
                     LGLLADPRAESAVRQGVTTLISGNCGSSPFPVAEEMLEELKSVARSEFDVEMDWRDLR
                     GFFTRLEKGKTAVNYATLVGHGAVRGAAMGFSDRPPAPAELDRMRELVAENMDAGAFG
                     LSTGLEYTPGSFADSDEIAALCRIVAERDGVHATHMRDEGDALIEALREAIETARRAG
                     VRLQISHLKTAFPRNWSKIDEVLGLLGQAGKDGLVVRADRYPYIAGSTGLSINFPAWA
                     REGTTGDFLSRLKDPALDGRLREYAAGREKKFGSWDKVVIAGVSSEKNKRVEGLDVLA
                     AAGRAGKPPYEFMRDLIIEERDRVDAVLFMMSEDNLRRILAHPQVGIGSDSSIRAPYG
                     ILASGKPHPRVYGTFPRVLNKYVRAEHLVSLREMIQKITSAPAGMFGLAGRGAVREEA
                     FADLVVINPETVADRATWSDPHQFPAGIDLVVVNGAVTVRDGEHTGALAGRVLRKTRT
                     KA"
     gene            complement(5893..7101)
                     /locus_tag="PKV64_04590"
     CDS             complement(5893..7101)
                     /locus_tag="PKV64_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN16392.1"
                     /translation="MMKLQEITPATGSRVFHEVARGVYAGDPNWIAPLDMDVEAVFDP
                     GKNGAFKQGEAIRWVLRDDAGRAAGRIAAFVDLEKAKRKDPAEGGIGFFECPDDPAAA
                     ALMFDAAKEWLAARGVEAMDGSVNFGANFNHWGVLVEGFMPQGYGMPYNKPYYAGLFE
                     GYGFREYFRQFSYHKNLAEPFPERMVKFAEHTIKKRTYEFRHYEKKRANRYIADLVEV
                     LNRTWADYMEDYQPVTPEEIGRIIKSAGPVIEEEFVWFAYKDGRPIGVVVTFPDVNQV
                     LARFDGRLNLWKTFRFLRLLKGKTITRNRVFLAGVIPEFQNSNVIGPLFLHFVRAVEA
                     RPHYREIELSWVGDYNPRMRKIYEQIGGVHKKTHVTYRYLFDRGRPFVRFTNEGGNSA
                     LRKDAVGKGA"
     gene            complement(7106..7558)
                     /locus_tag="PKV64_04595"
     CDS             complement(7106..7558)
                     /locus_tag="PKV64_04595"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPN16393.1"
                     /translation="MKNPFRPKWLVRIDEDTIRRAIAEAERRTSGEIRVSISRYFWGP
                     VRPVAERAFRRLRMAETRNRNGILFFLVPSRRRFVILGDEGIHAQAGPSFWTETAAAL
                     SGRFRKGEFTEGLAAGIRTAGEILAAHFPYNRATDVNELSDEIDFGKG"
     gene            complement(7571..8335)
                     /locus_tag="PKV64_04600"
     CDS             complement(7571..8335)
                     /locus_tag="PKV64_04600"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPN16394.1"
                     /translation="MRRRFSPRSGRGVFLASALCLACGFFPAAVQAVQGARIPPPPAR
                     WATDEAGFISPAVRAEIDFRLESFERETGRQVLLYISRTTGDVPLEDFAARVFGAWKV
                     GRKGMEDGLVLFLFADDRAVRIEVGYGLEGVVTDAIAGRVIQNILIPGIRRGDPDGAV
                     RAAVTALLETISGKPAAEPGETESADKPAEGEIPAVFLIIAGVLFLILLITNPRLALF
                     LLYTLLSGGGGRGGGGGGGFRGGGGRSGGGGASGRW"
     gene            complement(8361..9008)
                     /locus_tag="PKV64_04605"
     CDS             complement(8361..9008)
                     /locus_tag="PKV64_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPN16395.1"
                     /translation="MSNETPQIPASPRPKRRFGALGVLGVIVVLAVIIGLWIAGVYNG
                     LNRLDQDVRAKWGQVENVYQRRADLIPNLVETVKGAANFEKETYTAVTEARAKVGQIR
                     LDADVVNDPEAFAKFEQAQGELGSAISRLLVAVEAYPELKATQNFRDLQVQLEGTENR
                     IAQERRVFNESAQAFNTKRMSFPTNLVAGFFGNRFAEKSYFKAQPGAETAPAVKF"
     gene            complement(9217..11514)
                     /locus_tag="PKV64_04610"
     CDS             complement(9217..11514)
                     /locus_tag="PKV64_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPN16396.1"
                     /translation="MKSREFTRCLVFGGVLALLSGVLSAQGTQADYERAFQLQEKSRA
                     AALNITGPVVWTEGTNRFCYRKTVRGGFEFILVDADHIVKRPAFDHEKLAASLSRATG
                     EKHDARNLPFDRVVFVDKERAVEFEFGDGKWKCDLAAYACVKIGPSSRARPSPFAPAP
                     PPSRNPIPSPDGKWEAFIRDHNVYVRSADRKDEFPMSLEGIAGDEYNWESLVWAPDSN
                     KLAAFRIRPGARRKIQYVESSPADQLQPKYHAIEYLKPGDPLDEFQPVLFHVDTKKQF
                     IVDKSLFPNPYFPNTNFNMRYSKAYLAWRKDSRAFTFEYNQRGHQVYRVIEVDGRSGQ
                     ARALVDEECRTFFSYYGKLYRYDVQDGREIIWMSERDGWNHLYLYDGATGQVKNQITR
                     GEWVVRGVDRVDEENRRIFFRAGGMVPGKDPYLLHSYRINFDGTGLTPLTETDGNHTV
                     VFSPDLKYFVDTWSRIDLPPVVELRRTADRRLLMDVERADIQELLKMGYRMPEVFSAK
                     GRDGQTDIWGVVFRPRSFDPSRKYPVIEYIYAGPHDSFVPKSFSPFHSMQALAELGFI
                     VVQCDGMGTSNRSKAFHDVCWKDIADGGFPDRIPWIKALAAKDPSCDISRVGIYGHSA
                     GGQNTLTALLFHPDFYKAGVSSCGCHDNRMDKISWNEQWMGWPIGPEYAASSNVDNAW
                     RLQGNLLLSVGELDTNVDPASTMQVVNALIKANKVFDLIVLPGQGHTTGGTYGDRKRY
                     DFFVRHLLGVEPPEWNKEKIPSGNQ"
     gene            complement(11684..12400)
                     /locus_tag="PKV64_04615"
     CDS             complement(11684..12400)
                     /locus_tag="PKV64_04615"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPN16397.1"
                     /translation="MKEPSIINLASLKDQVYEYLRNQIKVGELRPGSHINIEETSKQL
                     GVSRTPLKDALLQLEMEGFVTILPRKGVLVRQLTIEDIKEYYEIIGALESTAFLSGYD
                     KINATIVQKMKNLNSEMIEAIGANNFTLYFQKNLEYHDIFLGLCKNKNLAKITDNLKR
                     RLYHFPRREGYLKQWEESSIREHVELTRLIEEGKKEEAAAFIRDVHWSFKIQEKFLKK
                     LFQKQEGYVPLVEKSGESRE"
     gene            complement(12566..14647)
                     /locus_tag="PKV64_04620"
     CDS             complement(12566..14647)
                     /locus_tag="PKV64_04620"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPN16398.1"
                     /translation="MRFDSATRTRAFVFAAVLIFSVSLLRADQAAAESPAEKHGRWLE
                     EEVVYLIAPIEKEIFLKLKSDRERDLFIEAFWKQRDPTPGTDENEFKKEHYRRLSHVN
                     RMFGRTTTKPGWMTDRGRMYIILGEPTDIQRYESKSDLFPAEVWFYQGKGDLGFPNGF
                     SLVFFQEGGVGEYRLYSPLKDGPQALLAYDQGNPFDYSGAYLKLREIEPALASVSISF
                     VPGEDSSGMGRPSLVSDMLVQNIEAYPEKIVDETYARKFFEYKDIVEVDYTANYIRSD
                     SLVKVIKDASGVYFVHYAIEPERLSIEAGRGGFQANLKIFGSVRTPEGKNVFQFEKKI
                     SLAFDQDQIENINRRPYSIQDMFPLIPGEFKLSILLKNETSKEFTSLEQDLVIPGEDD
                     RLQMTSLLLGYAAKKQEAEADKLRPFQFGSVQVQFPGNRTFTRNDTLIAVFQVHEIHG
                     DFKEKGEVRYVIMRDEDEMLRLSRKAGDYADLPDCFEEIPLGKFPPGHYRLLLSLAME
                     GREIISQREDFILTYLETIPRSWIHSRILPDADDPVYSYFIGTQLFNVGKIEAARNAL
                     EKAFRKKPDSYEYAINLAKACLRLADDRKIEEVLKPFLSLRTPPEYDVLFLLGTACKN
                     MGKWDQAVDYFNQAVTHYGVNPNLLNVLGTCYFQMGDHREALRAWEKSLEINANQPQI
                     QKNVNVLKGKK"
     gene            15264..17951
                     /locus_tag="PKV64_04625"
     CDS             15264..17951
                     /locus_tag="PKV64_04625"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN16399.1"
                     /translation="MHRKLKAAFCLLSLAVALAGIAAAQTRGTSAIVGKVVDQDGAPL
                     PGVSIVVTSPNLMGSRAAASDEEGQYRLTVLPIGTYSLEATLQGFNTVQKTNIVLHTG
                     ITATIPIEMTPAAQDHEIVVQGVAPLIDLQDTGLSKTVITKELLTNVPTSRNLTEIFA
                     FASGSQGDYSYGGSRASNSYQLDGVELVDAWYGSGVYTAPIDYEVVEEVEILGLGAPA
                     EYGNFTGSVANIITKSGGNTLSADLQFYYKNFDWHSKDGINKSDPYWALVPNAPISSL
                     TDPSFHLGGPIIQDKLWFFTGVEYYLRKERITALNKTLVNQFPKFFAKLTYQLDEKNK
                     FQTFFEVHNNKALDRLTSAFYAPEVNEDFLTPTYMGNLNYLHMFSPSTFLEFKVAGYT
                     MDNVYNPSQGKDVSGHYDVYTGMYTRNVWWNSLWQASRIGTSLSLTSHVDDFILGSHD
                     IKVGFEFERSHGAGQRNINGGIMFEDYNGEPYRARAGYTKQDAFNNRFSFYAQDSWKI
                     SDSLVLNPGLRVDIIRGSIPGADDSYKTVYKPTGIEPRIGFIWDIFKNHKTALKAHYG
                     RYQEGTKTYNFAQASAEADDIYYSVGPNWVGLTELYRIKPDNLYSIDPNIKHPSMDQF
                     AAGIEHVLGSDITVKMSYVYKKWFNFVEPVELGCTFVPVSYTDPETQQAITVYNNVNP
                     AAAKYFITNPQKGKDIGAAYPDIVSITPERKYHAFEVGLTKRFSNRWQLATSYVYSHE
                     EGSYSSSSLLGRSTQYLDPNNQIFGYGEFASSIPHIFKIQGTYVLPLDINLSGFFVFL
                     AGSPWGRTIPVKLSQGSVNVRTEPVGDRRLPSRSNMDLRLEKSFSLGAFRLSVLADVF
                     NLFNEVIQINVYNRAGTNFGKPTSVTAPRTIRLGLKISY"
     gene            18121..20349
                     /locus_tag="PKV64_04630"
     CDS             18121..20349
                     /locus_tag="PKV64_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPN16400.1"
                     /translation="MTPRPRLRLILQAAGAAVFLLGAWTAAFPAVQGRGKTEAGINVL
                     LVTMDTTRADRLGCYGYAKALTPRLDALASAGVRFDRAYAQAPLTLPSHCSLMTGTVP
                     AYHRVHNNGFYYLDPRRTTLAEILKKDGYATAAFVSSFTVDSRFGLDQGFDVYDDDFQ
                     EGEILKSFRSERTADKVYESFARWLDENAGRKFFCWLHFFDPHLPYQPPSDFRHPRSD
                     SPYDAEIAYMDEFIGKSIDALRRNGVLGRTLIVLCGDHGEGLGERKEIDHGLFLYDNT
                     VRVPLIMYWENRLPQGRVVSSRVRLIDLVPTILTLAGLAPPKESQGKNLVPLMKGKKG
                     IDLPVYLESYYPRENYGWSEITGLVDGDWKYLQSPKPELFNLALDPREKRNVLLQEPG
                     IARDMRKKLQESAEKLSSEKDGGKRRLSPEEEERLRSLGYLGRPSDGRSEKALPDPKD
                     KIEDYLLYFRGNLHETRGEYSEAEKCYREFLRLNPDVPGNFVNLGFLYMKMNRVEEAV
                     HVLEEGLRSHPDSVLILSRLMNFYLKAERDEDALAAGRILLAVDPRYFDALFLSGSIQ
                     AKKGEWKAALGFYRRALDIEPENKALRMRIGLSLAALGEYENALAVYEILWNQYPEDE
                     AVCLELSRVYESLGRIGPARGALKRLLDVRPSAELGYAYAFLSEKAGDLKEAVFWLKN
                     YLETTAEKRTKRKSEAEAVLRRWEERLKSGSGPREFPSPRRGKRPRAAQRPSGPEDRA
                     EP"
     gene            complement(20373..21374)
                     /locus_tag="PKV64_04635"
     CDS             complement(20373..21374)
                     /locus_tag="PKV64_04635"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN16401.1"
                     /translation="MRVIPQNRYSPWRIFLVLLALTVLLVPGARAQMGKGRINGTIAD
                     ESGRPLEKALIVARHLESEKALESLSDKKGRFAMGGMRSGRWRITITKEGYEGSFLDV
                     EISQLKANPPFALILKPAAAAGSGESEDAEALETGNRLMEQEKYDEALAVFNEFLARR
                     PDVYHVRLNIGTCYLEQGDLAKAEAEYRLVLDKARENGGNDKKSLSTISKALTGLGET
                     SMKKDDFEAAGNYFSQALDISPNDEVAAYNVAEIFFSHQKVDEAIRYLELSIRINPAW
                     SKPYMKLGYAYLNKGDFDKSLGCFETFMKMDPENPAVPAVSKTIAAIEGMKRSPAVS"
     gene            complement(21819..23099)
                     /locus_tag="PKV64_04640"
     CDS             complement(21819..23099)
                     /locus_tag="PKV64_04640"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HPN16402.1"
                     /translation="MAVRVATIIMAGGQGSRLYPLTKVRSKPAVPIAGRFRIIDISIS
                     NCIHSDYRKIFILTQFASESLHRHIFSTYRFDSFHKDFIAILAAKQTLDNRGWFQGTA
                     DAVRQNLSSVEGAGDLFLILSGDHLYRMDYRKFVEGHLKTGADITISVIPVDREQVPN
                     LGVLQVDQNNRIIEFKEKPREAETIAALAVPPDVFTANGIDGRGRTHVASMGIYLFNW
                     KVLKSLLEGSTAEDFGRQIIPQAIHEHRVFAHFFDGYWEDIGTVQSFFEANIGLTHPL
                     PRFNFYDEDKPVFSIPRFLPGSKILDADVHESLLCEGSIINRAFIRRSIIGIRSRIGE
                     GSRLERTVMMGADYYESEAELREAAGRGLPPVGVGRDCEIRNAILDKNARIGDGVKMI
                     NAAGIQNRECGDVCIVDGIIVVPKNSFVPSGTIV"
     gene            23249..23695
                     /locus_tag="PKV64_04645"
     CDS             23249..23695
                     /locus_tag="PKV64_04645"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPN16403.1"
                     /translation="MEEDVLEKRTCSRFRIPGATIAFRKEKGLFKKSAYGAEFLPMLD
                     ISRGGIRFLSQEMVELDTPIHLSIQVPGDPAPLKMDGIVRWFASNPGQSYKYQLGVQF
                     LPYGDKKDMNYPGNLVKIISYEQKFMDETTIIKDETGGRHDGTFEV"
     gene            complement(23726..25798)
                     /locus_tag="PKV64_04650"
     CDS             complement(23726..25798)
                     /locus_tag="PKV64_04650"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HPN16404.1"
                     /translation="MRKSALAPVFILVLSLAFPFSLSAALPAEERPLAPEPLRRAVAE
                     AVSGEIAFRYTVRISQFDRIQANAGWHDAAAWIRNELLRMGYADAIIEGWPSNGSTRY
                     FTYKTPIGWKAESAWLWMVEPRRERLCDYEEVPLTLIAHSGPGHFEAELVDVGAGTEA
                     ASFQGKDVRGKFVLAYGRSPEVMREACWKRGALGVVSYYPPDVRPGYPNMIRYTAFWP
                     LWEERDKMPVGFNVSKNQGAMLQRLLAEGKRVVLRAHVGSEFFETSVEALSASLPGTD
                     AAAGEILILGHLCHPQPSANDNASGSGGMLEIARALKALVESETLPAPRRTIRFLWIP
                     EFAGTVPYIKAHLEQTRKTLAALNCDMIGEDLHLTGGMFTITATPDSNPDVLTDVAVH
                     FARMVDDLGLISLNGSTHPFAWRVEPFSGGSDHWIYNDGALKVPSVMFGHGDTFHHTS
                     LDTPDKVDPTELRRVAALTLGIAYYLASAGEPEALDMARLVVRNGLGRLAAETGDALG
                     ALAAADAAGLPAAYKQALNVLERAAARERAAADSASRYAPAGSASAGEIRRLAGPLSA
                     LERALGEEAKRIYADRCRLFGLKAVFPAASDGEKAAAGIIPVRPREFVCPVESDFLVE
                     KLGPEVMGRIRLSGNAAYEALNHVDGKRSVADIARAVSASYGPQNIEDVVEYFRVLAE
                     AGLIVLGR"
     gene            complement(25903..>27427)
                     /gene="glyS"
                     /locus_tag="PKV64_04655"
     CDS             complement(25903..>27427)
                     /gene="glyS"
                     /locus_tag="PKV64_04655"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HPN16405.1"
                     /translation="KLHAPDGIEVGSFEDYRAKLRERLVVIDAAERKAAILDRIAGLT
                     GPRAAELYPDPELLDELVNDVESPFVVMGSFPEEFLRLPLEVLSTAMREGQKLFSVVR
                     EGKQLPLFLGVADVTGDPKGFIRAGNERVLKARLADARFFWEQDLKAGFQKRAPLLEE
                     VVFQEKLGSYADKAGRLQKIAAYLRGKVGGCAAAKEMNLAAALCKVDLVTDMVREFPS
                     LQGTMGGLYARAEGLPETVARAVAEHYLPVTVDDSSPESPEGAVLSLADKLDSIVGVV
                     GLGIQTTGSSDPFGLRRNAQGVCKIILDRKISLSFSRFVEKAVSAYGDKIKRPKSEIV
                     DQLQEFFAGRLRYLLEREGLRYDLINAALGAGLDDMYGAAQRARALDALKNGPQFEPL
                     ILMAKRVNNILSGQTAPAVDPDRFVEKAERELYATFTIIRDNVAPMIAGGDFVQAQKI
                     VFRLHPALNAFFDKVMVMTEEMKLRRNRLALLQAIRKTLLQIADYSQVVVEGEKPAK"
BASE COUNT         5321 a         8612 c         8009 g         5485 t
ORIGIN      
        1 ccgtcgacgg cggaacctat cccctgcccg atccgttcat gattatcgcc acccagaatc
       61 ccatcgaata ccacgggacc ttccccctcc ccgaagccca gctcgaccgt tttctcctcc
      121 gggtgaagat cgggtaccct tcggccgaaa acgaaaaaga gatcatccgc ggccgcgacc
      181 tctaccacct ggcggcccgg ctcgagccgg ttctcaaccc ggccgaggtc ctcgccctcc
      241 aggaccaggt cgacggcatc cgggtcgacg agtccctgct cggctatatc gtctccctgg
      301 cttcggaaac gcgcaaaacc aaaggcatca agctcggcgt cagcccccgc ggctctctcc
      361 tcctccggcg ggccgcccaa gcccacgccc tgacctacgg ccgagatcac atcctgcccg
      421 acgacgtcaa gcgcgtcgcc gcgcccgtcc tcacccaccg catcgtcatc gagtcccagt
      481 ccttcgggtt gctgcgcatc cacgaatccg acacgatcat ccagggcgtc ctcgagcgcg
      541 tgcccatccc tctctaggcg gccgcggctc atgctgacgc gtctcggcgt ccgtttcctc
      601 gccgccgccg tcctggccct ggtcggcgcg gccaccagcg gcgccaacct tctttacctg
      661 atcgacggca tgctctggtc gatcctggcg gtctcctggt ggtggagcgg ccggaacctg
      721 cgaggaatcg gcgcccgggc cgtttttccc gagcagatgt tcgtcgacac ggaattcccc
      781 ctggccgtct tcctgagaaa agagataggc ggacgctccc atttcctgaa gctccggtcg
      841 ccctcccggg aagcctcgct cgccctgctc aagcggggga aggaaagcgc cgtgaccatc
      901 ccggcggcgt tcccccgccg cggcctcaac gaaatcgggg acatctggtt ggaaagcgac
      961 tacccgttcg gcctgttccg gagaaagcgg cgggtcaccg gactcgtcgg acttgtcttt
     1021 cccccggttt tcgaaatcta cggccgccgg gcctccccgg ccgtccgggg cgaggatgtc
     1081 tcgatcccga agcggggcgc gggcggggaa ttcttcggcc tccgggaata cgacgagggc
     1141 gaggacgccc gcctcatcaa ctggaagctg acggccaaga caggcaagcc cctcgtcaag
     1201 gaatacgccg agcaggtcgg caaccggatc acgatcacgg tcgccgacgc cgaggggccg
     1261 gacgcggagc gccaaatctc ggaggccgcc tccctggcca agtatttcat cgacgccggg
     1321 tccgacgtcc ggctccgaac gcccgaggcg acggtcgcct tcggccacgg gctcatccat
     1381 ctcggcctga tctttaaaac cctggctctt ctcggccggg gcaagatcgt cgagcgattc
     1441 gaaaagccgc tgccgaaaac aaaatccccg gcctttccgg gagaagatcc cctctctcct
     1501 ttcgcctatt caaccgttct gaccgccttc gcctcgctct tcctgatcga ggagctcaat
     1561 cccttggtcc tggcttctta cgccctcctg ttccccctcg cctggctggg cgaccggaaa
     1621 aagcggtatc ttctcccccg gccggcttgc gacgccctcg ccgccttgta cctgctgttc
     1681 ttcctcttcg tcgacctgcc tcgggccgga tccctggggg cggtgacccg cctggtcctt
     1741 ttcatcctgg gctacctcct gttcaatccg aaaacgggcc gggccccggc ccagctcctg
     1801 accgcctcat tcctcgtttt ctttctcgcc tccgggcaag ccttgagtct ttggtacttc
     1861 gccttttttc tggctttttt cctttccgcc ggggcttggc tgatcgaccg gcgcgatccc
     1921 cgcccccgcc cccgggaacg gacctggggc cggtccctgc tagcggtcac gggaatagcc
     1981 gtgatcctgg ccgccgccgc tttcgtcgtc cttccccggc cctacagcgc ccggatgagg
     2041 cagcttttag ccgttacggg actgacccgc ttccagcagt cgctccgaac tttttcgggc
     2101 atgtctgaac aggtcgagct gggatacctc ggcccgattc acaagaactc gtcccgggtc
     2161 atgcgggtga tgcccggcct cccggacggc tcccccgttc ccgagttcat ccacgtccgc
     2221 ggcgcggcat tcgatttctt cgacggccgc cgctggcgga aatcccccgt ctccttcacc
     2281 tcggaaatct ccgggcggtt ggtttcctcc cggcggacga tggcttggat ccggcgggaa
     2341 aggggatgga tctccgctcc aggctacgat cctcaaaagc cgttccgcga ggaaacctac
     2401 gttctctccc ccttcctcaa caccagcctc gtctttttcg ccggcccgcc cggggcgatc
     2461 gaggcgaatt atcaaggcat ctttttcgac ttcacggaca cggcctactt ccccggcgct
     2521 tatctcgagg gaatctccta ccgggttcgg acccaaggcc gggaaccctc cttctcccgg
     2581 tcgatccggg actacggcgg gctgctccaa tcccattttc tccaacagcc cgggccgaac
     2641 gagcgcctgc gccgcttggc cgtccaattc gccgggcggg ccggagacgc gcgcagccgg
     2701 gcggccgccg tcgaggcccg cctccgcggc ggctattcct actccctggc cgccgatctc
     2761 ggccgccaag acatcgacgg ttttcttttc gaatcgaagg ccggcaactg cgaatacttc
     2821 gccacggcca tgtgcctgtt gctccggcat ttggacatcc cctcgcgctt ggccgtcggc
     2881 tttctcggca gcgagtggaa cgaatacggg aaattcttcg acatccgcca gagcgacgcc
     2941 cacgcttggg ttgaggcgta cctgcccgag tccggctgga cgacgttcga tccgacgccg
     3001 cccgatttca cggagagcgg ggcgggctcc cttttctccc gcctctggag cgctctgaac
     3061 gacaagttcg aagacctcca attccgctgg taccgctatg tcgtgggcta cgacacgttc
     3121 acccagagga atttccttta caacatcgtc cgaaaagcct ccgaggcggc ggttcctctt
     3181 ctcttcgggc tggccgggtt ttcggccgct ctgtttctcc tcctacggcg gaaatcccgg
     3241 ggaacgcttc cggcctggaa gccctcccga aaaaagccgg aggattttta tgaaacgctg
     3301 ctcgtccgcc tcggccgggc cggttttccc cgccggcctt cccagacggc cgccgaatac
     3361 gccggggaga ccgggtccgc ccatccggaa ttgggcggat tgaccgggct ggcccgccgc
     3421 cattacgagc tccgctacgc cggccgggtc ttggaggaat ccgagcgggc ggagatcgcc
     3481 cggacatcaa acgacatcgc ggccgccgcc cgccgcctaa aacggcgccg ccggcgggac
     3541 atcaagggag gaaacgcatg aagtcttacc gccgggagct ttttttcgaa atccccaacc
     3601 gccggggatt cgtcaacatc accccgcagg tcgagtcctg tctccgggaa agcggcatcc
     3661 gcgaagggct gtgcctggtg aactccatgc acatcacctc gtcggttttc atcaacgacg
     3721 acgaaagcgg cctgcaccac gacttcgaag tctggctgga aaaactcgct ccccacgagc
     3781 cggtttcttc ctaccgccac aacggattcg aggacaacgc cgacgcccac ctcaagcgcc
     3841 aggtgatggg ccgggaagcc gtcgtcgcgg tgacgagcgg aaagctcgat ttcggcacct
     3901 gggaacagat cttttacggc gaattcgacg gacgacgtcg aaaaagagtt ctagtcaaga
     3961 ttatcggcga ataagaggaa tcggcgagga ggttaagtac tcccctcctt aagcgaagat
     4021 cccggcaaga ggccgagggg caatcccgtc cttcaatgcc gacgagaccc tgaaggcgga
     4081 ggaaattaaa aaggaagcgg ttcccggccg gcttggccgg ccgcgggttt aagctttcgt
     4141 ccgggtcttg cgaaggacac ggccggccag ggcgccggtg tgctctccgt cccggacggt
     4201 gacggcgccg ttgacgacga ccaagtcgat gcccgccggg aactggtggg gatcgctcca
     4261 ggtcgcccgg tcggcgacgg tttccgggtt gatgacgacc aggtcggcga acgcttcttc
     4321 ccggacggct ccccgcccgg ccaagccgaa catcccggcc ggggcggagg tgatcttctg
     4381 gatcatctcc cggagcgaaa ccaggtgctc cgcccggaca tatttattca ggacgcgagg
     4441 gaacgttccg tacacgcgcg gatggggctt tcccgaggcg aggattccat agggagcccg
     4501 gatcgaactg tccgagccga tccccacttg gggatgggcc aaaatccggc ggaggttgtc
     4561 ctccgacatc atgaacagga cggcgtcgac ccggtcccgc tcctcgatga tcaggtcccg
     4621 catgaattca tacggcggct tgccggctct gccggcggcg gccaggacat ccaggccctc
     4681 gacccgcttg tttttctccg aggagacgcc ggcgatgacg accttgtccc aagatccgaa
     4741 ctttttctcc cggccggcgg cgtattcccg gagccggccg tccaaggccg gatccttgag
     4801 ccgggacagg aaatcgccgg tggtcccctc gcgggcccag gccggaaaat tgatgctcaa
     4861 gccggtcgag ccggcgatat aaggataacg gtcggcccgg acgaccaagc cgtccttccc
     4921 ggcttggccg agaaggccga ggacctcgtc gatcttggac caattgcggg gaaaagccgt
     4981 cttgaggtgt gaaatctgga gacggacgcc ggctcgccgg gccgtctcga tcgcctcccg
     5041 gagcgcctcg atcaaggcat ccccctcgtc ccgcatgtgg gtggcgtgga ccccgtcccg
     5101 ctcggccacg atccggcaca aggcggcgat ttcgtcggaa tcggcgaagc ttcccggcgt
     5161 gtattccaag ccggtggaca atccgaacgc cccggcgtcc atgttttcgg cgacaagctc
     5221 ccgcatccgg tcaagctccg cgggagcggg cggccggtcg ctgaagccca tggccgcccc
     5281 gcggaccgct ccgtggccga cgagggtggc gtaattgacg gccgtttttc ccttttccag
     5341 gcgggtgaaa aaaccgcgga gatcccgcca gtccatctcg acgtcgaatt cggatcgggc
     5401 gacggatttc aattcctcaa gcatttcctc ggccacggga aacggcgagg acccgcaatt
     5461 gccgctgatg agagtagtca cgccttggcg gacggcgctt tccgcccggg gatcggcgag
     5521 aagacccagg tcggaatggt cgtgaacgtc gatgaatccg ggagtgacga ttcggcccgc
     5581 cgcctcgacg acgatccggc cgctccggcc ggggatcggg ccgacggtct tgatccgctc
     5641 gccggagacg ccgatgtcgg cctcgaaggg gtccccgccc aggccgtcat aaaccagacc
     5701 gcccttgatc acaagatcgt agtccttgcc ggccgcgcag ccctggagga tcaggccggc
     5761 gccgccgagc gcggcggccg ccgcggccgt ccccaccgat ttcaggaatt cccgccgatc
     5821 gatctcccgg ctcatcgatc cctcctcatt aaatcgcgat ccgcgccgcc tccccctcca
     5881 tccgttcgtt tttcaagccc ctttgccgac cgcgtccttg cggagggcgc tgtttccgcc
     5941 ctcgttggtg aagcggacga agggccggcc gcggtcgaag agataccggt acgtgacatg
     6001 ggtcttttta tggacgccgc cgatctgctc gtagatcttg cgcatccggg gattgtaatc
     6061 cccgacccag gacagctcga tctcccggta atgcggccgg gcttccacgg cccggacgaa
     6121 atggaggaag agcgggccga tgacgttgga attctgaaac tcggggatga cgccggccag
     6181 gaagacccgg ttgcgggtga tcgtctttcc cttcaggaga cggagaaacc ggaaggtctt
     6241 ccagaggttc aagcggccgt cgaaccgggc caggacctgg ttgacgtccg ggaaggtcac
     6301 gacgaccccg atcggccggc cgtctttata ggcgaaccag acgaattcct cctcgatgac
     6361 cggcccggcg gatttgatga tccgcccaat ctcctcgggc gtcaccggct ggtaatcctc
     6421 catgtaatcg gcccaggtcc ggttgaggac ctcgacgaga tcggcgatgt agcggttcgc
     6481 ccgcttcttc tcgtaatgac ggaattcgta ggttcttttc ttgatggtat gctcggcgaa
     6541 cttgaccatt cgctcgggaa acggctcggc caggttcttg tgataggaga attggcggaa
     6601 atattcccgg aatccgtagc cttcgaacag cccggcgtaa tagggcttgt tgtagggcat
     6661 gccgtagccc tggggcatga atccctcgac caggacgccc cagtggttga aattggctcc
     6721 gaagttgacg cttccgtcca tcgcctcgac gccgcgggcg gccagccatt ccttggccgc
     6781 gtcgaacatc aaggcggcgg cggccggatc gtcggggcac tcgaagaacc cgattccgcc
     6841 ctcggccgga tccttccgct tggccttctc gagatcgacg aacgccgcga tccgccccgc
     6901 ggctcgcccg gcatcgtccc gaagaaccca gcggatcgcc tcgccttgct tgaaggcgcc
     6961 gtttttcccc gggtcgaaga ccgcttccac gtccatatcc agcggggcga tccaattcgg
     7021 atcgccggcg tacacgccgc gggcgacttc gtggaagacg cgggaccccg tcgccggggt
     7081 gatttcctga agcttcatca ttcgctcatc ctttcccgaa atcaatctcg tcggaaagct
     7141 cgttcacgtc ggtcgcccgg ttgtaaggga aatgggcggc caggatttcg ccggcggtcc
     7201 ggatgccggc ggccaatccc tctgtgaatt cccccttgcg gaaccggcct gacagggccg
     7261 cggccgtctc cgtccaaaac gacggtccgg cctgggcgtg gatcccctcg tcgcccagga
     7321 tgacgaaacg gcggcgggag ggcacgagaa agaacagaat cccgttgcga ttccgggttt
     7381 cggccatccg gagccggcgg aaagcccgtt cggccaccgg ccggacgggt ccccagaaat
     7441 accgggaaat cgacacccgg atctcgccgg acgtgcgccg ctcggcctcg gcgatcgccc
     7501 gacggatcgt gtcctcgtcg atccggacca gccattttgg acgaaaggga tttttcatgg
     7561 ccttgctccg tcaccatctc cccgaagcgc cgcccccgcc ggagcggcct ccgccgccgc
     7621 ggaatccgcc gccgccgccg cctccccggc ctccgccgcc cgaaagcagg gtgtagagga
     7681 ggaagagggc caagcgggga ttggtgatca ggaggatcag aaacaggacc ccggcgatga
     7741 tcaggaaaac ggcggggatt tcgccttcgg cgggcttgtc cgcgctttcg gtttctcccg
     7801 gttccgcggc gggcttcccg gagatggtct ccaggagggc cgtcacggcc gcccggacgg
     7861 cgccgtcagg atcgccccgg cggatgccgg gaatcaggat gttctggatc acccggccgg
     7921 cgatcgcgtc ggtaaccacg ccttccaagc cgtagccgac ttcgatccgg acggcgcggt
     7981 cgtcggcaaa gaggaagagg accagcccgt cctccatgcc tttccggccg actttccagg
     8041 ccccgaacac ccgggccgca aaatcctcca gcgggacatc ccccgtcgtc cgggaaatgt
     8101 agagcaggac ttggcggccc gtctcccgct cgaacgactc gagccggaaa tcgatctcgg
     8161 cccggacggc cggggaaatg aatccggcct catcggtggc ccaacgggcg ggaggcggag
     8221 ggatccgggc gccctggacg gcttggacgg ccgccgggaa aaagccgcag gccaggcaca
     8281 gcgcggacgc gagaaaaacg cctcgcccgg aacgcgggct gaaccgtcgc ctcatcggac
     8341 cgttccgatt cggacgctcc tcagaatttc accgccgggg cggtttccgc gcccggctgg
     8401 gccttgaaat aggatttctc ggcgaaccga ttgccgaaaa atccggcgac caagttggtc
     8461 gggaagctca tccgcttggt gttgaaagcc tgggccgact cgttgaacac ccgccgctcc
     8521 tgggcgattc ggttttccgt tccctcgagc tgaacctgga ggtcgcggaa attctgggtc
     8581 gccttgagct cggggtaggc ctccacggcc acaagcaggc gggagatggc cgatcccagc
     8641 tcgccctggg cctgttcgaa cttggcgaag gcctcggggt cgttgaccac gtccgcgtcg
     8701 agccggatct ggccgacctt ggcccgggcc tcggtgacgg cggtatacgt ttccttctcg
     8761 aaattggccg cccctttgac cgtttccacc aggttcggga tcaggtccgc ccgccgctgg
     8821 tacacgtttt caacttggcc ccatttcgcc cgaacgtcct gatcgagccg gttgaggccg
     8881 ttgtaaacgc cggcgatcca aagcccgatg atgaccgcca gaacgacgat gacgcccagg
     8941 acgccgaggg cgccgaagcg gcgcttcgga cgcggggaag ccgggatttg aggtgtttcg
     9001 tttgacatga ttcgtctctc ccttcctgaa tatatacgag aaaagctcct cgccggttat
     9061 tgtatcggtc cgccggagaa gattcaacat cgcccgaagg aatcccggcc cgccggggac
     9121 cgggccgcgc cgcttaagcg ggcgcggccc cgggcctccg gcggtccgcg gaaaataagg
     9181 gaagaggacg tcccccgaaa aaggccgccg tccgggttat tgattcccgg acgggatttt
     9241 ctccttgttc cactcgggcg gctcgacgcc gagcaggtgc ctgacgaaga agtcgtagcg
     9301 cttccgatcg ccgtaggtcc cccccgtcgt atgcccctgg ccgggcaaga cgatcagatc
     9361 gaagacctta ttcgccttga tcagggcgtt gacgacctgc atcgtcgagg ccgggtcgac
     9421 gttcgtatcc agctccccga cggaaagcag aaggttcccc tggagccgcc aggcgttgtc
     9481 gacattcgag gaggcggcgt actcggggcc gatcggccag cccatccatt gttcattcca
     9541 ggaaatcttg tccatccggt tatcgtggca gccgcaggag gagacgccgg ccttgtagaa
     9601 atccgggtgg aaaagaagcg cggtgagggt gttctggcct cccgcggaat gcccgtagat
     9661 tccgacccgc gagatgtcgc aggagggatc cttggcggcc agggccttga tccagggaat
     9721 tcggtccggg aagccgccgt cggcgatatc cttccagcag acatcatgaa aagccttgga
     9781 ccggttcgag gttcccatcc cgtcgcattg gacgacgatg aagcccagct cggccaacgc
     9841 ttgcatggaa tggaaggggc tgaacgattt cggaacgaac gagtcgtggg gcccggcgta
     9901 aatgtactcg atcaccggat atttccgcga aggatcgaat gacctcggcc ggaagacgac
     9961 tccccaaatg tccgtttggc cgtcccgtcc cttggccgaa aaaacctcgg gcatccgata
    10021 ccccattttc agaagctcct ggatgtccgc ccgctcgacg tccatcagca gccgcctgtc
    10081 cgccgtgcgg cgcagctcga cgacgggggg aagatcgatc cgcgaccagg tgtcgacgaa
    10141 atatttcagg tccggcgaga agacgaccgt gtgatttcca tccgtctcgg tcaacggcgt
    10201 cagaccggtg ccgtcgaaat tgatccggta ggaatgcagg agatagggat cttttcccgg
    10261 aaccatcccg cccgcccgga aaaagatccg gcggttctct tcgtcgacgc gatccacgcc
    10321 gcggaccacc cactccccgc gggtgatctg gtttttcact tggccggtcg cgccgtcgta
    10381 aagatagaga tggttccagc cgtccctctc ggacatccag atgatttctc ttccatcctg
    10441 gacatcgtag cggtacagct tgccgtagta cgagaagaac gtccggcatt cctcgtcgac
    10501 gagggcgcgc gcttgaccgg accggccgtc gacctcgatc acccggtaaa cctggtggcc
    10561 gcgctggtta tactcgaagg tgaacgcccg gctgtccttc cgccaggcca gatacgcctt
    10621 ggaataccgc atgttgaagt tcgtgttcgg gaaatagggg ttgggaaaaa ggctcttgtc
    10681 gacgatgaat tgttttttcg tgtcgacgtg aaagaggacc ggttgaaatt cgtccagggg
    10741 atcccccggc ttcaggtatt cgatggcgtg gtatttgggc tggagctggt cggccggcga
    10801 cgattccacg tactggattt tccgacgcgc gccgggacgg atccgaaaag cggccagctt
    10861 gttcgaatcg ggagcccaga cgagggattc ccagttgtat tcatcgcccg cgatcccctc
    10921 aaggctcatt ggaaattcgt ctttccggtc cgcggatctc acgtagacgt tatgatcccg
    10981 gataaaggct tcccacttgc cgtcgggaga gggaatcggg ttcctggagg gaggcggcgc
    11041 cggagcgaaa ggggacggcc gcgcgcggga cgacgggccg attttcacgc aagcgtaggc
    11101 ggccagatcg catttccatt tcccgtctcc gaattcgaat tcaacggccc gttccttgtc
    11161 cacgaaaacg acgcggtcga acggaagatt ccgggcgtca tgcttttccc cggtcgcccg
    11221 ggagagcgag gcggcgagct tttcatgatc gaacgccggc cgcttgacga tgtgatccgc
    11281 gtccaccaaa atgaattcaa atcctccccg gaccgttttc cgataacaga accggttcgt
    11341 tccttccgtc caaacgacgg gacccgtgat gttcaaggcg gccgcccggc ttttttcctg
    11401 aagctggaac gcccgctcat agtccgcctg agtcccctgc gccgaaagca cgccggaaag
    11461 aagggccaag acgcccccaa aaaccaaaca ccttgtgaat tctcgcgatt tcatagttcc
    11521 tccttggaga aattgacgaa gacgagcttg gtttccgcgg atcctcgggg agcgcgggcc
    11581 gtcccggaaa ggacggcgcg ggaaaaaccc tccctcgact cgacgccatc aacgaaccgg
    11641 caagcccaaa cggggcgccg tgtttctcgt cgaggggaaa gcgtcattct ctcgattcgc
    11701 ccgatttttc gacgaggggg acatagcctt cttgtttttg aaaaagcttt ttcaggaatt
    11761 tctcttgaat cttgaacgac caatggacgt cccggatgaa ggcggccgcc tcctctttct
    11821 tcccctcctc gatgagccgg gtcagctcca cgtgctcccg gatggaggat tcctcccatt
    11881 gttttaaata tccttcccgg cggggaaaat ggtagagcct tcttttgagg ttatcggtga
    11941 ttttcgccaa gtttttgttt ttgcagaggc cgagaaaaat gtcatgatat tcgaggtttt
    12001 tttgaaagta caaagtgaag ttgttcgccc cgatcgcctc gatcatctcg gagttgagat
    12061 tcttcatttt ctggacgatc gtcgcgttga ttttgtcata accggagaga aacgccgtgc
    12121 tttccaaggc cccgatgatc tcgtaatact ccttgatgtc ctcgatcgta agctgccgga
    12181 caagcacgcc ttttctcggc agaatcgtca cgaacccctc catctcgagt tggagcaggg
    12241 cgtctttcag cggcgtccgg ctgaccccca attgcttgct cgtttcttcg atattgatgt
    12301 gggatccggg acgcaattcg ccgactttga tttgattccg cagatactca taaacctggt
    12361 ctttcaacga cgcgagattg atgatgctcg gttctttcac atcccccctc cagattaaaa
    12421 atatctcata tttaatttat atgagatttt aaaaccaaat gtcaagtctt gaaagagacg
    12481 agtgaatggt taatccctga tcaggactga aaaaagaatc atcgattgat ttccgccgcg
    12541 gcttccccat cccccggaaa ccgccttact ttttcccttt caagacattc acattctttt
    12601 ggatctgggg ctgatttgcg ttgatttcga gcgatttttc ccaagccctc aaggcttccc
    12661 gatggtcgcc catctggaaa taacatgtcc ccaagacatt gagaaggttg ggattgaccc
    12721 cgtaatgggt taccgcttga ttgaaataat caacggcctg gtcccatttt cccatgtttt
    12781 tacaggccgt ccccaataaa aacaagacgt catattcggg aggagtccgg aggctcagaa
    12841 acggcttcaa cacctcttcg atcttccgat catcggccag ccgcaggcag gccttggcca
    12901 gattgatcgc atattcgtag gaatcgggtt tctttcgaaa ggccttctcc aaggcgttcc
    12961 gggcggcctc gatcttgccg acattgaaaa gctgcgtgcc gatgaaatag gaatagaccg
    13021 gatcgtcggc gtccggcaga atccgggaat ggatccagga ccgggggatc gtttccaagt
    13081 acgtcaggat gaaatcctct cgttgagaga tgatctcccg gccttccatc gccagtgaaa
    13141 ggaggagccg gtaatgcccc ggcgggaatt tcccgagggg gatctcttca aaacaatcgg
    13201 gaagatcggc gtagtccccc gccttccgcg acaagcggag catctcgtcc tcgtcccgca
    13261 tgatcacgta gcggacctcg cctttctcct tgaagtcccc gtggatttcg tggacttgga
    13321 acacggcaat cagcgtgtcg ttcctcgtaa acgtccggtt cccggggaat tgaacttgaa
    13381 cggatccgaa ttgaaacggc cgaagcttgt ccgcctccgc ctcctgtttc ttcgcggcgt
    13441 aaccgagcaa aagagaggtc atctgcaggc gatcgtcctc cccggggatc accaggtctt
    13501 gttcgaggga ggtgaactcc ttggaggttt catttttcag gaggatggag agcttgaatt
    13561 cccccgggat gagaggaaac atatcttgga tgctgtaggg ccggcggttg atgttttcga
    13621 tctgatcctg atcgaaggcc aaggagatct tcttttcaaa ctgaaagacg tttttccctt
    13681 ccggggtccg gacggatccg aagatcttga gattcgcctg gaagccgccc ctccccgcct
    13741 cgatcgacag ccgctcgggc tcgatggcgt aatgaacgaa ataaaccccc gaggcgtcct
    13801 tgatcacttt caccagggaa tcgctgcgga tataattcgc cgtataatcc acttccacga
    13861 tgtctttgta ctcgaaaaac ttgcgggcgt acgtttcatc gacgattttt tcgggatacg
    13921 cttcgatatt ctggaccagc atatcggaga ccaggctggg acgccccatc cccgaggaat
    13981 cctctcctgg gacgaagctg atcgagacgg aagccaaggc cggttcgatt tccctcagct
    14041 ttaaatacgc gccggaataa tcgaacgggt tcccttgatc ataagccaag agggcctggg
    14101 ggccgtcttt caaggggctg tacagccggt attcgccgac ccccccctcc tggaaaaaca
    14161 cgagggagaa tccgttcggg aagccgagat cgccctttcc ctggtaaaac cagacttccg
    14221 cgggaaaaag atcggacttg ctttcatatc tctgaatgtc ggtgggctcg cccaaaatga
    14281 tgtacatgcg cccgcggtcc gtcatccagc cgggcttggt cgtcgtccgc ccgaacatcc
    14341 gattgacgtg gctcaaacgc cggtagtgct cttttttgaa ttcgttttca tccgtgccgg
    14401 gcgtcgggtc ccgttgtttc cagaacgcct cgataaaaag gtctctttcc cggtccgatt
    14461 tcagctttaa gaaaatctcc ttttcgatgg gagcgatgag gtaaacgact tcttcttcaa
    14521 gccatcggcc gtgtttttcg gccggacttt ccgccgcggc ctggtccgcc cggaggaggg
    14581 acacggagaa aatgagaacc gccgcaaaaa caaacgcacg ggttcttgtc gccgaatcaa
    14641 acctcatgat cttcatccgt ccatcccgtt tttggctttg atgcctcaaa aaacatggaa
    14701 agcacgccga attctccaaa gatttatctt atcccaacat tttgtgaatt tcaaatccgg
    14761 ctctctcgag gaaaagattt tcccatgtgc gccggttggg tttcattgac attttttctt
    14821 tattttcaat tacatacggg aacataccgg agaggttatt ggaaattcta acatttctgt
    14881 cttattcatg gaatcggtct ttcaaggctt gggcgtgttt tgaatcgatt tccccatatg
    14941 cgtatattta cattaatttc ttatatttat atgccatttc agaacgcatg aaaaatcacg
    15001 ccaaggcttg acaaaaagaa attgtttatt atatatatca gatgtaatat cgttaatgaa
    15061 acacttattg ttttgtatgt cgagcgtgga aacggggtcc gggagggctg tccggacgaa
    15121 aggaggggaa gaaaatgcgc ggaccgtcct gtgtccgcgt ttgttttttc agccgcaggc
    15181 ctcggatcga atgaggaaat cggaacacca aagaaagcgc cgaacgctaa aacgctcttg
    15241 gccgcttaaa caaggagggt aaagtgcatc gaaaattgaa agccgctttc tgccttttgt
    15301 cgttggcggt ggccttagcc ggaatcgccg cggcccaaac gcgcgggacg tcggccatcg
    15361 tcggcaaggt cgtcgatcag gacggggcgc cgctccccgg cgtcagcatc gtcgtcacgt
    15421 ccccgaattt gatgggaagc cgggcggccg cctcggatga ggaaggccaa taccggttga
    15481 cggtcctgcc catcggaaca tactctttgg aggcgactct ccaaggcttc aacaccgtcc
    15541 aaaaaaccaa catcgtgctg cacacgggga tcacggcgac catccccatc gaaatgactc
    15601 cggcagccca ggatcatgaa atcgtggttc aaggcgtcgc ccccctcatc gacttgcagg
    15661 acaccggtct gagcaaaacg gtgatcacca aggaactctt gaccaacgtc ccgacgtcgc
    15721 ggaatctgac ggaaatcttc gccttcgcct cgggctccca gggagattat tcttacggcg
    15781 gcagccgggc gtcgaactcc tatcagctgg acggggtgga attggtggac gcttggtacg
    15841 ggtccggcgt ctacacggcc cccattgatt acgaagtcgt ggaagaagtg gaaatcctcg
    15901 gcttgggcgc tccggctgaa tacgggaact tcaccggctc ggtcgccaac atcatcacca
    15961 aatccggcgg aaacaccctg agcgcggatt tgcaattcta ttacaagaac tttgattggc
    16021 acagcaagga cggcatcaac aaaagcgatc cctattgggc cctggtgccg aacgccccca
    16081 tttcctcgtt gacggacccg agctttcatt tgggcgggcc catcatccag gacaagcttt
    16141 ggtttttcac gggcgtcgag tattacctgc gcaaggaaag aatcacggcc ctgaacaaaa
    16201 ccctggtcaa tcagttcccc aagtttttcg ctaaattgac ctatcagctc gacgaaaaga
    16261 acaaattcca gacgttcttc gaggttcaca acaacaaggc cctcgaccgc ctgacgagcg
    16321 ccttctacgc ccccgaggtc aacgaggatt tcctcacgcc gacgtacatg gggaatctca
    16381 actaccttca catgttctcg ccttcgacct tcctcgagtt caaggtcgcc ggctacacga
    16441 tggataacgt ctacaacccc agccagggca aggatgtgtc cgggcattat gacgtctaca
    16501 cggggatgta cacccgcaac gtctggtgga actccttgtg gcaggcgagc cgcatcggga
    16561 cgtcgttgtc cctgaccagc catgtggacg acttcatcct cggctcccac gacatcaagg
    16621 tgggattcga atttgaaaga agccacgggg ccggacagcg caacatcaac ggcgggatca
    16681 tgttcgagga ttataacggg gagccctacc gggcgcgggc cggctatacc aagcaggacg
    16741 ccttcaacaa ccgtttttcc ttctacgccc aggactcctg gaaaatctcc gactccctcg
    16801 tcctcaatcc gggactgaga gtcgacatca ttcggggcag cattcccggc gcggatgaca
    16861 gctacaaaac cgtttacaag cccacgggca tcgaaccccg catcggcttc atttgggaca
    16921 tcttcaaaaa tcataaaacc gccctcaagg cccattacgg gagataccag gaagggacga
    16981 aaacctacaa tttcgcccaa gcgtcggcgg aagcggacga catctactac agcgtcgggc
    17041 cgaattgggt cggtttgacg gagctctacc ggatcaagcc ggacaatctg tattccatcg
    17101 atccgaacat caagcatccc tcgatggatc agttcgccgc cgggatcgag cacgtgctcg
    17161 gctcggacat caccgtcaag atgtcctatg tgtacaagaa atggttcaat ttcgtcgagc
    17221 ccgtcgaact cggatgcacg ttcgtcccgg tttcctacac ggatcccgaa acgcaacagg
    17281 cgattaccgt ttataataac gtgaatccgg ccgctgcgaa atatttcatc accaatcccc
    17341 aaaaaggaaa ggacatcggg gccgcctacc cggacatcgt ctccatcacc cctgaaagaa
    17401 aataccacgc cttcgaagtc ggattgacga aacgattctc caaccggtgg cagctggcca
    17461 cctcgtacgt gtacagccat gaggaaggaa gctattccag ctcctccctt ttggggagga
    17521 gcacccagta cctggatccg aacaaccaga tcttcggtta cggggaattc gcctccagca
    17581 ttccccatat tttcaagatc cagggaacct acgtcctccc gctggacatc aatctgagcg
    17641 gctttttcgt ctttctggcc ggctcgcctt ggggccggac gattcccgtg aagctctcgc
    17701 aaggaagcgt caatgtccga acggagcccg ttggagaccg ccggcttccc tcgcgcagca
    17761 acatggacct gaggctggaa aaatcattct ccctcggggc tttccggctt tccgtcctgg
    17821 cggacgtctt caacctgttc aatgaagtga tccaaatcaa cgtgtacaac cgcgccggga
    17881 cgaatttcgg aaaaccgacg agcgtgaccg ctccgagaac cattcgcctt ggattgaaaa
    17941 tcagctactg attttcttac tccccttccc gttaaaggcc tttcctcccc ggatcaaatc
    18001 cggggaggaa aggccatgtt tttttctccc gcctttgccg gcgccggccg cccccccggg
    18061 gatgaggatc gggagcgcgc ctttcttgtg agaggatttc cgttcaagta gaatggagcc
    18121 atgacccccc gcccccgtct ccgtctaata ctccaagccg ccggcgccgc cgtttttctc
    18181 ctcggcgctt ggaccgccgc ctttccggcc gttcaaggcc gcggtaaaac cgaagccgga
    18241 atcaacgtct tgctcgtcac gatggatacg acgcgggccg accggctggg ctgttacggc
    18301 tatgccaagg ccctcacccc ccggctggac gccctggctt cggccggggt ccgtttcgac
    18361 cgggcttacg cccaagcccc tctcaccctt ccgtcccatt gttccctcat gacgggcacc
    18421 gttccggcct atcatcgcgt ccacaacaac ggattctatt acctggatcc ccgccggacg
    18481 accctcgccg aaatcctgaa gaaagacggg tacgcgacgg cggcgttcgt ctcctcgttc
    18541 accgtcgact cccgcttcgg gctcgaccag ggattcgatg tttacgacga tgacttccag
    18601 gaaggggaaa tcctgaaaag cttccgttcc gagagaaccg ccgacaaggt ttatgaatcc
    18661 ttcgcccgct ggctcgacga aaacgccggc cggaaattct tttgctggct gcatttcttc
    18721 gatccccacc tcccctatca gcctccctcg gattttcgcc atccccgttc cgacagtccg
    18781 tatgacgccg aaatcgccta catggatgaa ttcatcggga agagcatcga cgccctgagg
    18841 aggaacggcg tcctcggccg aaccttgatc gttctctgcg gagatcacgg ggaggggctg
    18901 ggggaaagaa aagagatcga tcacggattg tttctctacg acaataccgt ccgtgtccct
    18961 ttgatcatgt attgggagaa ccgccttccc caagggcggg tggtctcgtc ccgggttcgg
    19021 ctgattgatc tcgttcccac gatcttgacc ctggccggcc tcgctccgcc gaaagagagc
    19081 caggggaaaa acctggttcc gctgatgaaa ggcaaaaagg gaattgatct tccggtctat
    19141 ctggaatcgt attatccccg ggaaaattac ggatggtcgg aaatcaccgg cctggtggac
    19201 ggcgattgga aatatctcca atcccccaaa cccgaattgt tcaacctcgc cctcgatccc
    19261 cgggaaaagc ggaacgtcct tctccaggag cccgggatcg cccgggacat gaggaaaaag
    19321 ctccaagaat cggccgaaaa gctctcttcg gaaaaggacg ggggaaaaag gcggctctcc
    19381 ccggaagaag aggagcgcct ccggtcgctc ggctacctgg ggcgtccctc ggacggccgt
    19441 tcggaaaaag cgctccccga tccgaaggat aaaatcgagg attatctcct gtatttccgg
    19501 ggcaatcttc atgagacgcg cggcgaatat tcggaggccg agaaatgtta tcgagaattc
    19561 ctccgtctca atcccgatgt ccccggcaat ttcgtcaatc tcgggttcct ctacatgaaa
    19621 atgaaccgcg tcgaagaggc ggttcacgtc ctcgaggagg gactccggag ccatcccgat
    19681 tccgtcctga tcctcagccg tttgatgaat ttttatctta aagcggagag ggacgaggac
    19741 gccctggccg ccggccgaat cctgttggcc gtggatcccc gttatttcga cgcgctgttc
    19801 ctttcgggaa gcatccaagc caagaagggc gaatggaagg ccgccctggg tttttaccga
    19861 agagccctgg atatcgagcc tgaaaacaag gcgctccgga tgagaatcgg cttgtccctg
    19921 gcggccttgg gcgaatacga aaacgccctg gccgtttacg aaatcctttg gaatcaatat
    19981 cctgaagacg aggccgtctg tctggagctc agccgcgtct atgaatccct gggacgaatc
    20041 ggtccggcga ggggagcttt gaagcggctt ctcgacgtcc gtccctcggc ggaattagga
    20101 tatgcctacg cctttctctc cgaaaaagcg ggggatctga aagaagccgt cttttggctg
    20161 aaaaactatc tcgaaacgac ggccgagaag cggacgaaaa gaaaaagcga ggccgaggct
    20221 gttctccgtc gatgggagga gcggctgaaa tccggttcag ggccgaggga attcccttcc
    20281 ccccggcggg gaaaacgccc ccgagcggcg caacggccct ccggcccgga ggaccgcgcc
    20341 gagccttaaa ggaaccggcc cctcgcggcg ttttacgaaa cggccgggct cctcttcatc
    20401 ccttcgatgg ccgcgatcgt cttgctcacg gccggcacgg ccgggttttc cggatccatt
    20461 ttcatgaacg tctcgaaaca gccgagggat ttatcaaaat cccccttgtt gagataggca
    20521 tatcccagct tcatataagg cttggaccaa gcggggttga tccggatgga tagctcgaga
    20581 taccggatcg cctcatcgac tttttggtgg gagaaaaaga tttccgccac gttgtaggcc
    20641 gccacttcgt cgttcgggga aatgtccaag gcttgggaaa aatagttccc ggcggcctcg
    20701 aaatcgtctt ttttcatcga cgtttccccc agtcccgtga gcgccttgga gatcgtcgac
    20761 aggctttttt tatcgtttcc gccgttttcc cgcgccttgt ccaagacgag ccggtattcc
    20821 gcttccgcct tcgccaggtc tccctgttcg aggtaacacg ttccgatatt gaggcggacg
    20881 tgatagacat cgggacgccg ggctaaaaat tcattgaaca cggccagggc ttcgtcgtac
    20941 ttttcctgtt ccatcaggcg atttccggtt tccagcgctt ccgcgtcttc cgactccccc
    21001 gagccggcgg ccgccgccgg cttcagaatc aaggcgaagg ggggattggc tttcaactgg
    21061 ctgatttcca catccaggaa ggatccctcg tatccctcct tggtgatcgt gatcctccat
    21121 cggcccgaac gcatccctcc catggcaaac cgcccttttt tatccgatag gctttcaagc
    21181 gctttttcag attccaggtg tcgggcgacg atcagcgctt tttccaaggg ccggccgctt
    21241 tcatcggcga tcgtcccgtt gattcgtcct ttgcccatct gcgcccgggc gccggggaca
    21301 agaagaacgg tcagcgccag caagaccagg aagatcctcc agggggaata ccggttttgg
    21361 ggaataacgc gcatgtccgc ctccttcagc ggagaccgag cccaataatc cgtctcgttt
    21421 tcttcaacgg aattttgatg atgcgttccg acgggacgca tccgttcgtc tccgcggaga
    21481 atgttcttta tatccttcgt gatcgaagga actctatttt atcatttttt ggctcttttt
    21541 caagaaaaag cctccgtttt cggaccgttc ccagctttga tgttctccca aagcccgtct
    21601 ctcccgccgt ctggccgggc ttcatttcag agggcggaaa gggatgatcc gggatttcct
    21661 ttatcgtccc ggcgttcccc cgaaggagat cggcggcccg cggagataag ggcggccggg
    21721 ggccgtccgg ctcgccggcc gttctcgaca cggctttaaa aaaccggttc gacacggaaa
    21781 gcgcgccgcc tcaacgccgc cgtcccggcg gatccccgtc agacaatcgt gccggacggc
    21841 acgaaggaat tcttcggaac gacgatgatg ccgtcgacga tgcagacgtc gccgcattcg
    21901 cggttctgaa tcccggccgc gttgatcatc ttgacgccgt cgccgatccg ggcgttcttg
    21961 tcgaggatgg cgttgcggat ctcgcaatcc cggccgacgc cgacgggagg cagcccccgg
    22021 ccggccgcct ctcggagctc cgcctccgac tcataataat ccgcgcccat catgaccgtc
    22081 cgctccaagc gggacccttc cccgatccgc gagcggatgc cgatgatcga ccggcggatg
    22141 aacgcccggt tgatgatgct tccctcgcac aggagggatt cgtggacatc ggcatcgagg
    22201 atcttggagc ccggcagaaa acgggggatg ctgaacaccg gcttgtcctc gtcataaaaa
    22261 ttgaaccgcg gaagcggatg agtcaggccg atgttggcct cgaaaaagct ctggaccgtc
    22321 ccgatatcct cccagtagcc gtcgaagaaa tgggcgaaga cccggtgctc gtggatcgcc
    22381 tggggaatga tctggcgccc gaaatcctcc gcggtcgagc cttccagcag gcttttcaga
    22441 accttccaat tgaagaggta gatgcccatc gaggccacgt gggtccggcc gcggccgtcg
    22501 atgccgttgg ccgtgaagac gtccggcggg acggccaagg ccgcgatcgt ttcggcctcc
    22561 cggggctttt ccttgaattc gatgatccgg ttattctgat ccacttggag cacgcccagg
    22621 tttggaacct gctcccggtc caccggaatc accgagatcg tgatatcggc cccggtttta
    22681 aggtggccct cgacgaactt ccggtaatcc atccggtaaa gatgatcccc cgaaaggatc
    22741 aggaaaagat cgcccgcgcc ctcgacggaa gagaggttct gccggacggc gtcggccgtc
    22801 ccctggaacc acccccggtt gtccagcgtc tgtttggccg ccaggatggc gatgaaatcc
    22861 ttgtggaagc tgtcgaaccg gtaggtggaa aagatatggc ggtggaggga ttcgctggcg
    22921 aactgcgtca ggatgaagat cttccggtaa tcggagtgaa tgcagttgga aatcgagatg
    22981 tcgatgatcc ggaaccgccc ggcgatgggg acggccggct tgctccggac cttggtcagc
    23041 gggtaaaggc ggctcccttg gccgccggcc atgatgatcg tcgcgacacg aacggccatg
    23101 gacgtctccc ttccgccccc attatattcc tttttcaggg ggaaagtcag ggccggaaag
    23161 ccgccgcccc gcctctcgtc gactttacat ttatttcggc ttgggatatc ttatgcctgc
    23221 gaatcggaaa aaacgtcagg gaggcgtcat ggaggaagac gttttggaaa aacgcacctg
    23281 ttcccggttc cgcatccccg gggcgacgat cgcgttccgg aaggaaaagg ggctcttcaa
    23341 gaagagcgcc tacggcgcgg agtttctccc gatgctggac atcagccggg gcggcatccg
    23401 ctttctttcc caagaaatgg tcgaactgga cactcccatt cacctcagca tccaggttcc
    23461 cggagatccc gctcccctga aaatggacgg catcgtccgc tggttcgcca gcaatcccgg
    23521 ccagagctat aaataccagc tcggcgtcca gttcctgccg tacggcgata aaaaggacat
    23581 gaactatccg gggaacctcg tcaagatcat ctcgtacgag cagaaattca tggacgagac
    23641 gaccatcatc aaggacgaga ccggcggccg ccacgacggc acgttcgaag tctgatcgtc
    23701 ccgcccgggc cgcaacggcc gatccttacc tccccaagac gatcaatccc gcctccgcca
    23761 aaacccggaa gtactcgacg acgtcctcga tgttctgagg cccataggac gccgaaacgg
    23821 cccgggcgat gtccgcgacg ctccgtttcc cgtcgacatg gttcaaggct tcgtaggccg
    23881 cgttgccgct caggcggatc cggcccatga cctccgggcc gagcttttca accaggaaat
    23941 cggactccac gggacaaacg aactcccggg gacgaaccgg gatgatcccg gccgcggcct
    24001 tttccccgtc ggaggcggcc ggaaaaacgg ccttgaggcc gaaaagccgg cagcggtcgg
    24061 cgtaaatcct cttggcttct tcgcccagcg cccgctccag ggcggacaag ggcccggcca
    24121 agcggcggat ctcgccggcc gaggccgacc cggcgggcgc gtagcgagag gcggaatccg
    24181 ccgccgcccg ctcccgggcc gccgcccgct caagaacgtt caaggcctgc ttgtaagcgg
    24241 ccggcaaacc ggccgcgtcc gcggcggcga gagcgccgag ggcgtcgccc gtctcggccg
    24301 ccagccgccc gagcccgttg cggacgacga gccgggccat gtccagggct tcgggctccc
    24361 cggccgaggc caggtaatag gcgattccaa gcgtcaaggc cgcaaccctc cgaagctccg
    24421 tgggatcgac tttatcgggc gtgtccaggc tggtgtgatg gaacgtgtcg ccgtgaccga
    24481 acatcaccga ggggactttc agcgcgccgt cgttgtagat ccagtgatcg cttcccccgc
    24541 tgaagggctc cacccgccag gcgaaaggat gggtgctgcc gttcaggctg atcaggccga
    24601 gatcgtccac catccgggcg aaatggaccg ccacgtcggt caagacatcc gggttggaat
    24661 ccggcgtcgc ggtgatggtg aacatccccc cggtcaggtg aaggtcctcg ccgatcatgt
    24721 cgcagttgag ggcggccagg gtcttccgcg tctgttcaag atgggctttg atatacggca
    24781 cggttccggc gaattccgga atccaaagaa aacggatggt ccggcgcggg gccggaagcg
    24841 tttccgactc gaccagggcc ttgagggccc gggcgatttc cagcatgccg ccgctgccgc
    24901 tggcgttgtc gttggcgctg ggctgggggt ggcataagtg gccgagaatg aggatttccc
    24961 cggccgccgc gtctgtcccg ggaagggagg cgctcagggc ttcgacggat gtctcgaaaa
    25021 attccgaacc gacgtgagcc ctcaggacga cccgcttccc ttccgccagg agccgctgga
    25081 gcatggctcc ctggttcttg ctgacattga agccgaccgg catcttgtcc cgctcttccc
    25141 agagaggcca gaaagccgtg tagcggatca tgttcggata tccgggccgg acgtcgggcg
    25201 gataatagga gacgaccccc agggcgcccc gcttccagca ggcctcgcgc atgacctcgg
    25261 gactccggcc gtacgccaga acgaacttgc cccgcacatc cttcccctgg aacgaggccg
    25321 cttccgtccc cgctccgacg tcgaccagct cggcttcgaa atgcccggga ccggaatggg
    25381 cgatcagcgt cagcgggact tcctcgtagt cacagagccg ttcccgccgc ggctcgacca
    25441 tccagagcca ggccgactcg gccttccagc cgatgggcgt tttatacgtg aagtaacggg
    25501 tcgacccgtt ggacggccag ccctcgatga tcgcgtcggc gtaacccatc cgcagaagct
    25561 cgttccggat ccaggcggcg gcgtcgtgcc agccggcgtt ggcctggatg cggtcgaact
    25621 gcgagatccg gacggtgtag cggaaagcga tctccccgga aaccgcttcg gccacggccc
    25681 ggcggagtgg ttccggggcg agggggcgct cttccgcggg caaagccgcg ctcagggaga
    25741 agggaaacgc caagctcagg acaagaataa agaccggcgc caaagcggac ttgcgcatgg
    25801 atgatcctcc tggaagtaga atcgccggaa tgaggcggcg gatggcatga ggagaaagaa
    25861 tacctcaatc aaaaaagcgg aacaagggac ggccggatcg ccttatttcg ccggcttttc
    25921 cccctcgacc acgacctggg agtaatcggc gatctggagc agcgtcttgc ggatcgcctg
    25981 gagaagcgcc agccggtttc tccggagctt catctcctcg gtcatgacca tcactttgtc
    26041 gaagaaggcg ttgagggccg gatggaggcg gaagacgatt ttctgggctt ggacgaaatc
    26101 tcccccggcg atcatcgggg cgacattgtc ccggatgatc gtgaatgtcg cgtaaagctc
    26161 ccgctccgcc ttttcgacga agcggtccgg atcgacggcc ggggccgttt ggccggataa
    26221 aatgttgttg acccgcttgg ccatcaggat cagcggctcg aactgggggc cgttcttcag
    26281 ggcgtccagg gcccgcgccc tctgggccgc gccgtacatg tcgtccaggc cggctcccag
    26341 ggcggcgttg atcaaatcgt agcggaggcc ttcccgctcc agcaggtacc ggagccggcc
    26401 ggcgaagaat tcttgaagtt ggtccacgat ctcggatttg gggcgcttga tcttgtcccc
    26461 gtaggccgag acggccttct ccacgaagcg gctgaaggat aggctgattt tccggtcgag
    26521 gatgatcttg caaacgccct gggcgttgcg ccgcaggccg aaaggatcac tcgagcccgt
    26581 tgtctggatg cccagaccga cgaccccgac gatcgaatcg agcttgtcag ccagggagag
    26641 gaccgccccc tccggactct ccggtgagga atcgtcgacc gtcaccggca ggtaatgctc
    26701 ggccacggcc cgggcgacgg tctcgggcag cccctcggcc cgggcgtaaa gaccgcccat
    26761 cgtcccctgg agagacggga actcgcggac catgtccgtc accaggtcga ccttgcagag
    26821 ggccgcggca agattcatct ccttggccgc cgcgcacccc ccgactttcc cgcggaggta
    26881 agcggcgatc ttctgaagcc gaccggcctt gtcggcgtag gaaccgagct tttcctggaa
    26941 aacgacctct tccaacaggg gggcgcgctt ctggaagccg gccttgagat cctgctccca
    27001 gaagaaccgg gcgtcggcca gccgggcttt gaggacgcgc tcgtttcctg cccggatgaa
    27061 gcctttcgga tcgccggtga cgtcagccac gcccaggaac agcggaagct gttttccctc
    27121 cctgacgacc gaaaagagct tctgcccttc ccgcatggcc gtgctcagaa cctcgagagg
    27181 cagccggaga aactcctcgg gaaacgagcc catcacgaca aacggcgatt cgacgtcgtt
    27241 gaccagctcg tccaggagct ccggatctgg gtacagctcg gccgcccgcg ggccggtgag
    27301 cccggcgatc cggtcgagga tggcggcttt cctctcggcg gcgtcgatga cgacgagccg
    27361 ctcccgcaat ttcgcccggt aatcctcgaa ggatccgacc tcgatcccgt cgggggcatg
    27421 aagcttg
//
LOCUS       DAOOXH010000025        27023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_1573_length_27023_cov_7.756118, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000025 DAOOXH010000000
VERSION     DAOOXH010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 27023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27023
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1573_length_27023_cov_7.756118"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="PKV64_04660"
     CDS             <1..1486
                     /locus_tag="PKV64_04660"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPN16406.1"
                     /translation="LDGRTLMPGFVEGHSHPFMNMLVENMAVDIRPVRYARGPQVMDV
                     INAAVARAAPGEEIVFFGWDPVIQSGLKNPTRRELDALAPHHSLLIIGNSGHVAFANS
                     RALEAAGITRDTPDPTGPMGGSFGHSEDGELNGRLDQGDPILAVMTPILKNRLSDPQK
                     AVELVRKGWRQCAGNGITTVDDSMIDEQYLHLYRETAGLFPSLRIRGYAVHYKTWKPF
                     EGDDLIKVNGAKVFVDGTPWTGTLFLTKPYPANPLTLEVMELPPDYIQKPYISPADLK
                     TLVDDCLKEKRTLEIHAQGDAAIDMALETVEEGLERYPWPDHRFRIAHAPMIRDDQLE
                     RAKRLGVQITFLMSHVKYWGDILPTMVGEERGRRWMPAGSAERIGVNYAFHFDGPVAP
                     NKPFSVLETAVVRKTVGGRILGPEERVTIDEAIRAYTINPAYQLFMEKEVGSLEPGKF
                     ADMIIVSENPRTIPPHQLSDIKVLAAYVNGAEIFRAPEAIPPNR"
     gene            complement(1563..2369)
                     /locus_tag="PKV64_04665"
     CDS             complement(1563..2369)
                     /locus_tag="PKV64_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN16407.1"
                     /translation="MRNAPFVSVYKFSARLVRRSRRSRLLGALGLLPVILAVILQITR
                     AAAAGGGLDGLEVFSNFVMGFSLQFLVLLLSLFFGTAITADEVDNKTLTYPATRPVSK
                     AAFILGKYASSVSFLLVLVLTSTVVSFLILMIDRLSYGTAWTFLGRSLAALGFGTICY
                     TALFTFAGTFLRKSVLIGLVFCFGWENIVQYFPGMTQKFTIMHWLKSLLPGLGGGMGS
                     TEGGGLTAFLLFRHEPSPAGTAVAVLLLLTAVFLALAVAVFSRKEYLFDE"
     gene            complement(2371..3306)
                     /locus_tag="PKV64_04670"
     CDS             complement(2371..3306)
                     /locus_tag="PKV64_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN16408.1"
                     /translation="MTAGGRILRADKLGKWYGNVLGLSDVSIEIGPGITGLLGPNGAG
                     KSTFMKLITGQLRPSIGTVTIAGQPVWNNPGIFKSVGFCPEQDAFYEDTTGREFLSGL
                     LALGGFPAAEIRERTARALAFVELQADADRLIRGYSRGMRQRLKISQALAHEPEILIL
                     DEPLNGLDPLSKRKVIRLIKEYRTEGRTVLVSSHVLPEIEALTRSIILIHQGKILAQG
                     DIHFIRDLIDAHPHIVAVRTGRGRALAAKFVEEDDVRSVRFDEAQGTVLFETRNRDRF
                     FERLTRLAAEEGWEIEEITSPDDNLQSVFDYLVGK"
     gene            complement(3303..4175)
                     /locus_tag="PKV64_04675"
     CDS             complement(3303..4175)
                     /locus_tag="PKV64_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16409.1"
                     /translation="MEPIAERGYTHWEGTLGTGRRPWRVIAGQAVRLAWKRRFFKPLF
                     FFSLLPAVVFSGGIYISERLGDFQEMLKGADKFLTINPDYFQAYYGSSFLFFMMLMIM
                     VGGGAGLIADDLRHNALPLYFSRPLRQRDYIGGKGAALAFFLLLLTLAPGIVFLLLKL
                     IFSGSAKILSDFPWLPFSFLAWSVFVSAFFSLFTLLLSSLGRNRRFASILILAAYFLS
                     DVFFGIFYAIFREPAFCLVSLQRNLRQASASFLGAKPPMEVPGIYSILILAAIMAAAV
                     LVLRRKIRGTEVIR"
     gene            complement(4175..5086)
                     /locus_tag="PKV64_04680"
     CDS             complement(4175..5086)
                     /locus_tag="PKV64_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN16410.1"
                     /translation="MNIELRDVRFSYGPIRALDGVTLQIGEGACGLLGPNGAGKSTLL
                     KVLLGFLRPEAGEGLVLGHDIRKDSFGLRRVVGYMPEDDCFIPGLDAVSFTANFGELS
                     GMPRAEAMKRAHDVLYYVGLGESRYRLLETYSAGMKQRLKLAQALVHDPKILFLDEPT
                     ANLDPQGRSEILELISDITSKKDLHVLISSHILTDIEQTCSALVILNKGRVAAQGDLA
                     SLREVRFSLYEMKVKGEKGSFLEGLRRGGCRIEETEDGLLKAYLPEEMSRRDVFRLAA
                     AAGVQVRHFVKSRTSLEDLFARTVGVD"
     gene            complement(5132..6739)
                     /locus_tag="PKV64_04685"
     CDS             complement(5132..6739)
                     /locus_tag="PKV64_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPN16411.1"
                     /translation="MNEKESYPRTALGEAIRRAYRSGEEDEALLPLIATDSSGRPAGR
                     IGSGDSLIFYDIRGEREVEITRSLIEPGFSHFPTAGDLRLHYTTLIEYAPGLPVRVAF
                     PPEGRLRNTLSEAVTRAGLSLLKISESEKAIHVGFFLNGKSEDVFPGEDRIVIPSPEG
                     VAYYDAVPEMSAGAVAAAVEAAAADPKHALVVANFANVDVVGHIENREAILKAVESVD
                     AAVGRVVRAARVNKATLLVTADHGTVENWLYPDGKIDTGHTKSPVPFLFIDFAEPAVA
                     GVTLRDGGELADVAPTVLDLLGLPKPAEMTGESLFASRGDVVPPRRKIILLILDGWGL
                     APDGEGNLIAAAATPHFDELWDACPRTELQASGEAVGMPPGTVGNSEAGHLHIGAGRR
                     VFLDRVRIDRAIADGTFYRNEAFVEAMTEARTKGKALHLMGIISHYSSHGTIRHLFAL
                     LEAARRQGLRDVYVHGFLGRRGERPESGALYVEKVEDKCRELGLGRVVTVLGRYWSLD
                     REENWDRIEKAYRAMVFGDGRAVPPHP"
     gene            6994..8028
                     /locus_tag="PKV64_04690"
     CDS             6994..8028
                     /locus_tag="PKV64_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16412.1"
                     /translation="MDNPRRDLPVLLLHNLDPAWTGEERSEAEEDVAALGDALKSLGH
                     PVEVAAVEDARLAKRLAAFDPREHIVFNWCEELPGVPHSEPEVAAVMERLGFVFTGSS
                     CSVLTLSHDKPRVKRLLDAARLPTPPWAVFHTPKAGDWNIFPAIVKAAHEHCSIGISP
                     DSVVTSPAELEHRVAYVLETHKQAALVEEFINGREFHGAAWGNGTVSMLPIAEMDYGN
                     LIDIRDRLFTYEAKYVPGSRLYEGIDLRVPADLDEDARAELERIVLATYRVTGCRDYG
                     RIDVRYLDGVFQVIDVNPNPDINPQTSMTWAAAEIGFSYGEMGSRIVNLAAARHPVFR
                     RNDSDAPVAE"
     gene            complement(8038..9558)
                     /locus_tag="PKV64_04695"
     CDS             complement(8038..9558)
                     /locus_tag="PKV64_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16413.1"
                     /translation="MRAGWMMLGAAVWGLMMGCAPAGRGPSTAPKPLGGLRYYLARDF
                     VVVEADVTEFVETRIEAGADGWLKPVTESGIFAVKASVGLETSADPSRPYMLDAALGG
                     AADQSLAVRVGNNGLLTSAEFAAKDKSAEILVQIARLVGAAAGTAAGFRGGLNAGTKK
                     MDASGAGARRRLESLSLENRYYLQESSLGRELWEEADRLENELAGRKEALRRVRDEAV
                     ETTEPARYSLAEKRAAFLEGSIRFLEKQTEAARAAFASAAVKFSAAEGIGRTTRERKV
                     RRHLDITDLPPSAALTGVFDEAGITDALEKAYPRVLDLYRETGIAVAFDPDPARPEIL
                     DRPAADPSPPGKNEIRIFYREPVPGFLRIYSWREAVDREAGRFRPVFVRLDEKLVFAV
                     HPQTPVLCAGFERKAFSSRNMQLTVNAQGRVVGLSKSAASIPVAAAAGTAAAFEEIRE
                     SAAASLKEIRDIMDALRKLSLGELETQAALLKLRLDKLKKEAEIEKFEDDGPPPGE"
     gene            complement(9757..10521)
                     /locus_tag="PKV64_04700"
     CDS             complement(9757..10521)
                     /locus_tag="PKV64_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPN16414.1"
                     /translation="MAQGLAVAAGLRRVDPSSYGGSVPASGCWGAEEDVDRIIRFISS
                     RGFECLALKTAEATAAALLGNLRRAARLCRPGDFLFFYFAGHGGQRPDSGGEEEDGLD
                     ELLLAYDRPVADDELDACWREFAGGVRILVIIDACHSGTVYKNFGGRRRVSPRPAFRP
                     AHLPMKASLIHIGAVPDGERSPAYRGGGAFTLALCEALTSDSPGDYRDLVRRIERNLD
                     PPYPRLMLCGPGAEEFAGEKPFWLGEESPRATVFTE"
     gene            10629..>11094
                     /locus_tag="PKV64_04705"
     CDS             10629..>11094
                     /locus_tag="PKV64_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPN16415.1"
                     /translation="MKKRILVLLFAAGFLAAALSAQALKPLSIDDFLKIKRLTDPQLS
                     PCGKWVAYTVTVMDKEANKGDSDIWVAPLAGGDPRLLASSPAADHTPRWSPDGKRIAF
                     ISSRGGTPQIWTVAAEGGEPVQLTRLSTGASGVIWSPDGRLLAFASSVYPEAA"
     assembly_gap    11095..11194
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11195..12743
                     /locus_tag="PKV64_04710"
     CDS             <11195..12743
                     /locus_tag="PKV64_04710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPN16416.1"
                     /translation="EAAAAGKVKARLFDTLLVRHWNAWSDGTRSHVFVVPAAGGPAVD
                     LTPGDFDTPPIALGGSQDYVFSPDGKEIAFVRNIDPELKKGLGTNNDVFVVPSAGGEI
                     RRITTNKANDHSPLYSPDGRYLAYLAMARPGFEADKQSLMIYDRTAGTTVNLTEALDR
                     SVGEMLWTPDSAALYFVCDDWGRKAVFRAALDGRKVERVLEGHNLSSLQLTPDGTTFV
                     FARQAMNQPADVFTCRLADKTLSRVTDINRDLLAGLDMNPAEEITFLGAGDEPVHGWL
                     LKPPAFDASKKYPLVMLIHGGPQGAWTDEFHFRWNAQLFAAPGYVVAMINFHGSTGYG
                     QAFTDSISGDWGGKPYTDIAKGMGYIHGRYPFIDISRNAAAGGSYGGYMINWIESRGT
                     KVFNCLVSHSGVFDLRSMYGATEELWFPEWEYLGTPWTSPEQYRKFSPSSTVADFKTP
                     CLVIHGAKDFRVPLEQGLQMYTSLQRMNVPSKFLYFPDEDHFILKPQNSELWYRTIWE
                     WFAEYLK"
     gene            complement(12753..14081)
                     /locus_tag="PKV64_04715"
     CDS             complement(12753..14081)
                     /locus_tag="PKV64_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16417.1"
                     /translation="MSPNQKPAAALTSRTALNYWLPLQATWLMMAVEGPFVAAIIARL
                     ADPKINLAAFGVATSLAWIVESPIVMMLSASNALVQDKIAFRRLRRFSTVLNAAVTAV
                     MILLLLPPVFRWIARGVIGLPEDVARLSAQSMIFLILWPAAIGYRRFYQGILIRGGKP
                     RGVAWGTAVRLSTMAATGVVLALAVRLPGACVGGGALGIGVLAEAAASRFMARGLVRR
                     LRSQSDEECAFGRALTLDKIGRFYVPLAMTSFLSFSVHPLTSFFLGRSRMPIESLAVV
                     PVIMGLAFIFRTGGIAVQEVVIALAGDRGEHKILLRRMAGTIGLAGTLGMAAVVFSPL
                     APVWMGTVSGLSPELAGFALLPARLLVLLPFLEAVLAYRRGILVRARDTNPIAAAVLV
                     QIAMVAAVFWLTVGPLGMIGATAAGIALTAGYLGGNGTLFILSRRGPVRS"
     gene            14334..14924
                     /locus_tag="PKV64_04720"
     CDS             14334..14924
                     /locus_tag="PKV64_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPN16418.1"
                     /translation="MTGEARDRIVAEARRLFYQFGFRRIAMDEIAANLRMSKKTLYAL
                     FPSKKELIQAVVESILEPNLARVSALARNEKSVAGFFSGSIAVFHGLSRSISEPMMAD
                     MRTMPDVWSKVEARRREALMGLRQVLERGKRSGEIRPDLHVDLFLRIYLLMINAVGNP
                     AVILELNMKPSELAGQLGGLFLKGILNDKRRTGGAS"
     gene            14921..15901
                     /locus_tag="PKV64_04725"
     CDS             14921..15901
                     /locus_tag="PKV64_04725"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPN16419.1"
                     /translation="MKRGLGLGVLIVLCACGGRSVRNEIILTGSVESDPVRVSPLVGG
                     RLLEVAAVEGTRVGAGGIIARIDATDLELQLRQAETAVRTAETQLALVRKGVRSEDLA
                     TAREQVRQAEIAVDKAERERRRLARLYEEGSIPAKALDDAAVQADSAQSVLEQARKQY
                     EKAEAGARKEEIEAAEAPRDQARAAADILRKKIGDCLVLSPVAGTVLHRLAEPGEVVA
                     PGTTLAIVADLSTVKITAYVPERDLGSIALGMSASLRADAFPGRSFPASVSRIADEAE
                     FTPKTIQTRDERVKTVYRIELTAPNPEGVFKPGMPVDVVLARPESRPAAR"
     gene            15917..16846
                     /locus_tag="PKV64_04730"
     CDS             15917..16846
                     /locus_tag="PKV64_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN16420.1"
                     /translation="MAEEAALQASSLRFSFGQTEALGGVDLEVARGEMFGLVGPDGAG
                     KTTLIRAFCGLLAPDSGACRVLGLDTVRDRARLIRRIGYLSQRFSLYGDLSVGENLDF
                     FAEIHGRKDSAGRKRRLLEEMDLLRFRKRPAENLSGGMKQKLALACTLIHAPEILFLD
                     EPTNGVDPVARRDFWNILAGLSAGGVTVVISTPYLDEAEHCGRVAFMAGGRIAAVETP
                     AGAKELVRERICEIVCAPVFRAREAAGLVPGVVSVHAYGDKIRAALAAGAEPDALRAA
                     VEKNARISAFRMVRPSLEDVFIHLLRRNRGTTA"
     gene            16843..17760
                     /locus_tag="PKV64_04735"
     CDS             16843..17760
                     /locus_tag="PKV64_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN16421.1"
                     /translation="MTAAPAVEVRGLEKRFGRFRAVKAIDFSIGRGEIFGFIGANGAG
                     KTTTIKMLCGLLPPDGGRAVVAGIDVAADPEGVKRRIGYMCQKFALYGDLTATENVDF
                     FGGIYGLPPERLRARRAGLFRLLDLESRAGRRAADLPRGFQQRLALACAVVHEPAVIF
                     LDEPTAGVDPVQRRAFWDLIYGFASAGTAVFVTTHFLDEAEYCHRVGFIAEGELAAVD
                     TPAELKGRLTGWTIYELRTADPAGTLSAIQALPGVRSAALFGETIHVLASEAADPADL
                     FGRLSGPAGYDPIPPTLEDVFLHLAGKTP"
     gene            17757..18881
                     /locus_tag="PKV64_04740"
     CDS             17757..18881
                     /locus_tag="PKV64_04740"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN16422.1"
                     /translation="MKLAAVVKKEFLQIRRDPLTLFMLVFFPTALLLFFGYILSFDVR
                     NVRLGVLDLDGSDSSRAFIRQLTGGGYFQLETMFAGRGEADTALARGSVMAAVVVPKG
                     FGEALSGGKTAAVQGLIDGSDSRKAAIVQGYLQAYATAFSRQALGDWAARLGRRPVVP
                     VEVEGRVWYNPELKSSLHLLAGLIVFIFMITGTISTSLSVVRERERGTMEQLLVSPLG
                     AGTVILGKTLPYLAVSAVSTVIILIVSRFAFGLAVKGSIALLALASLLFLLAALGWGI
                     LISTVTRSQQVAYFAAALSSILPALLLSDFVFPISGMPAVIRAITAVVPAKYFVRLLR
                     GIMMRGAGFAAGWADLGALALFSAFTLTLAAVRLKKVKLA"
     gene            18889..20004
                     /locus_tag="PKV64_04745"
     CDS             18889..20004
                     /locus_tag="PKV64_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN16423.1"
                     /translation="MLGKIIHKELLQLRRDKRMFPILIAAPVLQLLILGYAANFDIAD
                     MPAAVCDLDRTPESRGFLNGFFGSGYFLRALDVPGGADLDPVLEAGRAQIGLVIPRGF
                     GRALGRGEGTKIQVIVNGSDPNSGINGYAFALQATARYSTAVLIERMAASGVASIPAV
                     DLRTRIWFNPELKTRYFMVPALSGLILMIISIMLTAMTLVREKETGTIEMLVVTPVKK
                     LSMLAGKLLPYLVIGLVEVGLIALVAVFWFEVPFRGSAGLLVLGSLLFLFNTLGLGLL
                     VSAVSRTQQRAMMIVMFAVMMPFIYLSGFIFPIEAMPAAFQAVSRFIPLTHYLEIVRG
                     LFLKGSGFADLAGALAALAGSGTVIFALSVLRFRKFL"
     gene            complement(20007..21893)
                     /locus_tag="PKV64_04750"
     CDS             complement(20007..21893)
                     /locus_tag="PKV64_04750"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HPN16424.1"
                     /translation="MRKPSWRLIIGLIVLAFWAARVPPAAAAERDGGVLPSRLLGIFG
                     GMGPEATANLYQLIVQQTPAQKDQDHIPTLMFSFPQVPDRTTCILNNDPSIIPYLVQG
                     VQFLEKAGASVIAIPCNTAHFYHDQMQAAVKIPVLHMIREAVDEVLRLRPDVKKVGLL
                     ATSGTLRTGLYEKEFRARGVETLVPPESAQEERIMRAVAGIKAGRPKPENAALLAEPA
                     REMAARGAEIIVLGCTEIPLAFDETCVTVPVVNATLALARAAVRTFRELEAAPAAAAG
                     RRGRFRDFGLKTGILPPGKWNAITDVAGVEVGQVTLIEGTDVRTGVTAILPHGGNLFQ
                     DKVPAAVHVANGFGKLAGQSQVEELGSLETPIVLTNTLNVSQAVEGLIEYTLAQPGNE
                     NVRSVNAVAGETNDGFLNDIRRRPVRPAHVLQALRSAASGPVAEGNVGAGTGTVCFGF
                     KGGIGTSSRALPAKLGGYTVGVLVQTNYGGVLQVAGEPAGILLDRHYLKDELAGSRAD
                     GSCMIVVATDAPLRARNLERLAKRAVFGLARTGSVGSNGSGDYVIAFSTSPDMRIPHE
                     SGSAVLSGGELRDDEMTPLFLAAAEAAEEAVLNSLFQAETMEGCGGRKVEALPLERLL
                     PFLK"
     gene            complement(22067..22990)
                     /locus_tag="PKV64_04755"
     CDS             complement(22067..22990)
                     /locus_tag="PKV64_04755"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPN16425.1"
                     /translation="MDDRRKRPGAAKTAVRAVWLGILVVAAFAGFEESGLQIKVLPGI
                     PLEFSFRALAPGEAVLVKPLADPSIRRIDLRFRTQKRRVEFDGKRRTILLGIDLSARA
                     QAYFLEFTVERRDGSRESFFRKLSVSSKEFPSTRLTVAPGMAKAPPEEAERIEREAAL
                     VASVLSVVSPEWLGTGSFATPLPEGEPFPNFGQRRLYNNAVASIHSGVDISAPAGTPA
                     RAANSGRVVLAANLYYSGNTVIIDHGLGVFTYYGHFSKILVKRGQPVKKGEVIAKVGS
                     TGRSTGPHLHWSVRVLDSRVDPFSLAALPLE"
     gene            complement(23009..23845)
                     /locus_tag="PKV64_04760"
     CDS             complement(23009..23845)
                     /locus_tag="PKV64_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPN16426.1"
                     /translation="MPRRLRFPAAFLLVLALAWGAGYAPAEDIGPAAANPDWRLNGAY
                     LKKAASDILAAGTAPFRWRGGDLLTFAAFAGTTGLLFAFDGDFYQAIQDRKTDASRDA
                     SKIISKIGNGGYLAGFLAVLYGGGEILDSRSLRRTALTGLESLIAASAVNLAFKIAAG
                     RARPYAGEGPESFHPFSFKGRYTSFASGDAAGAFAVATTIALNSDSFWIDSLAYLAAG
                     LAAFYRVHDRKHWPSDVFAGSAIGYFTARAVDALNRNGDGLRVSFQAGPGRGAVILAV
                     AF"
     gene            complement(23838..25115)
                     /locus_tag="PKV64_04765"
     CDS             complement(23838..25115)
                     /locus_tag="PKV64_04765"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HPN16427.1"
                     /translation="MYAIYSALLALALAVLLPLYCVRLRIRRKKPLHLKERLAFVRPV
                     PRGGRPFVWIHAVSVGEVLSLQNLVQEIRKAHPGWEIGFSVLTQSGYALARDRIRDVD
                     HIFFVPFDLGWCVRRVFSRLRPALLVLAESEYWPRLLREAGRRGCPVLVVNGRVSART
                     FGRLRRFPWAADFLLAKVARFLVQTPLDGERLEKLGVPSSKIQMAGNLKCDTRPPELT
                     PAELAAVKTGLGIPPDAKVVVAGSVHPGEEDLLLEAFRAARLRRPEVRMILAPRHPDK
                     FGGFETSRPGAGFVIQKKTALDASRPWDILLLDTIGELARMYAAGDAAFIGGSLIPWG
                     GQNLLEPAFYGKPIVFGPHMKNFAVLAEAFEGGGGAKTVRTEDDIAAFFAFDDPAGME
                     EMGRRSKEILRSLQGATRRSLAAMESLLEERDA"
     gene            25262..25435
                     /locus_tag="PKV64_04770"
     CDS             25262..25435
                     /locus_tag="PKV64_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16428.1"
                     /translation="MAEETKPEGEKTAKRIKINKLSVSEIDAKLNEVKTSQGGLGSRY
                     ARQLLLRKKALGK"
     gene            25559..25825
                     /gene="rpsO"
                     /locus_tag="PKV64_04775"
     CDS             25559..25825
                     /gene="rpsO"
                     /locus_tag="PKV64_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPN16429.1"
                     /translation="MLTKEKKTHLVTENRIHPTDTGSVEVQVALLSERINALTKHMSA
                     AKKDHSSRTGLMKIVSQRKRLLIYLKREDPARYEKLIQRLGLRK"
     gene            25843..>27023
                     /locus_tag="PKV64_04780"
     CDS             25843..>27023
                     /locus_tag="PKV64_04780"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPN16430.1"
                     /translation="MKMIIPSFSLEINNRTLSVEFGKMGKQADGSATVRYGETVIFVA
                     ATSKKEAREDKPFLPLVVDYRENTYAAGKIPGGFFKREGRPSEREILVSRLIDRPVRS
                     LFPDGYHNETQIVALLLSADLENEPDTLGLIGASTALTLSEIPFTTPFGAVKVGLIDG
                     KYVINPTATELESSPLNLLVVGIEEGVCMIEAGAKEIDEETMLGAIEAALEPIRRIIQ
                     AQKDLAARIGVKKRPFEPKPVDEAKLAAIEGKIGAALKAAIQTPGKKAHEAAVEAVQK
                     ELIAGIPEENEDERAEAKLFFEKIYEKYARRMILEDGRRVDGRGFDDIRPITIDVGFL
                     PRTHGSALFTRGETQALATVTLGTVQDAQRLDGLGEETQKRFMLHYNFPPFSVGEVGF
                     LR"
BASE COUNT         5067 a         8825 c         8461 g         4570 t
ORIGIN      
        1 cctggacggc aggacgctga tgccggggtt cgtggaaggg cattcgcatc ctttcatgaa
       61 tatgcttgtc gaaaacatgg ccgtcgacat ccgccccgtc cggtacgccc gcggtccgca
      121 agtcatggac gtcattaacg ccgccgtcgc ccgggccgct ccgggcgaag agatcgtttt
      181 tttcggttgg gatccggtca ttcagagcgg attgaaaaat ccgacgagac gggaactcga
      241 tgccttggcg cctcatcatt ccctcctgat catcggcaac agcggccacg tcgctttcgc
      301 caattcccgg gctttggaag cggccggaat cacccgggac acgcctgatc cgaccgggcc
      361 gatgggagga tccttcgggc attccgaaga cggggaattg aacggccggc tcgaccaggg
      421 ggacccgatt ctcgcggtga tgacgccgat cctgaagaac cggctctcgg acccgcaaaa
      481 ggccgtggag ctggtgagaa agggatggcg gcaatgcgcc gggaacggca tcaccacggt
      541 cgacgacagc atgatcgacg aacaatatct ccacctgtac cgggaaacgg ccggcctctt
      601 cccttctctc cgcatccggg ggtatgccgt ccattacaag acttggaaac ctttcgaagg
      661 cgacgatctc atcaaggtga acggggcgaa ggttttcgtc gacggaacgc cgtggaccgg
      721 gaccttgttt ctgaccaagc cctatcccgc caatcccctg actctcgagg tgatggagct
      781 cccgcccgat tacattcaga agccttacat cagcccggcc gacctgaaaa ccctggtcga
      841 cgattgtttg aaagaaaaaa ggactctgga gattcatgcc cagggcgacg cggcgatcga
      901 catggccctc gaaaccgtcg aggagggctt ggagcgatat ccctggcccg atcaccgctt
      961 ccgcatcgcc catgccccga tgatccggga cgaccagctc gaacgggcca agagactggg
     1021 cgttcaaatc acgttcttga tgtcgcacgt caagtactgg ggggatatcc tccctaccat
     1081 ggtcggagaa gaacgcgggc gccgttggat gccggccggc tccgccgaga ggatcggggt
     1141 gaattatgcg ttccattttg acggcccggt cgctcccaac aagccgtttt ccgttctcga
     1201 aacggcggtc gtcagaaaga ccgtgggcgg ccggatcctg gggccggagg agcgcgtcac
     1261 gatcgacgag gccatccgcg cctatacgat caatcccgcc tatcaactgt ttatggaaaa
     1321 agaggtcggc agcctcgagc ccggaaaatt cgcggacatg atcatcgtga gcgagaaccc
     1381 ccggaccatt ccccctcatc aactgtccga catcaaagtg cttgccgcct acgtgaacgg
     1441 agcggagatt ttcagggccc cggaagcgat ccctccgaac cgataatcac ggcggacgat
     1501 cgagggaggg atcgcggttt ccggaagctt tctctctttc tcccggggag ggccggccgc
     1561 cctcattcgt cgaagaggta ctccttgcgg gaaaagaccg cgacggccaa ggccaggaag
     1621 acggccgtga ggagaagaag gacggctacg gccgttcccg ccgggctggg ctcgtgccgg
     1681 aacaggagga acgccgtcaa tccgcctccc tccgtcgatc ccatccctcc tcccaagccc
     1741 ggaagaagcg acttcagcca gtgcatgatg gtgaatttct gggtcatccc ggggaagtac
     1801 tggacgatgt tttcccagcc gaagcagaac accaatccga tgaggacgga tttccggagg
     1861 aacgtgccgg cgaaggtaaa gagagccgtg tagcagatcg tgccgaagcc cagggcggcg
     1921 aggctccggc cgaggaacgt ccaggccgtt ccatacgaca gccggtcgat catgaggatc
     1981 aggaacgaaa cgaccgtgga cgtcagcact aggacgagaa gaaaggatac ggaagaggcg
     2041 tattttccga gaatgaaggc cgccttggaa acgggccggg tggccgggta ggtcaaggtt
     2101 ttgttgtcga cctcgtcggc cgtgatcgcc gtgccgaaaa acaaggacag gagaaggacc
     2161 aggaactgca gggagaagcc catgacgaag ttggaaaaga cttccaatcc gtccaagccg
     2221 ccgccggcgg cggccgcccg ggtgatctgg aggatgaccg ccaggatcac gggaagaagg
     2281 ccgagcgcgc ccaggagccg ggagcgccgg gagcgccgga ccagccgggc gctgaactta
     2341 tagacggaaa caaacggcgc attccgcatc ttatttcccc acaagataat cgaacaccga
     2401 ctggaggttg tcgtcgggcg aagtgatttc ctcgatctcc cacccctcct ccgccgccag
     2461 ccttgtcagg cgctcgaaga accggtcccg gttccgcgtc tcgaagagga ccgtcccctg
     2521 cgcctcgtcg aagcggacgc ttctcacgtc gtcctcctcg acgaacttcg cggccagggc
     2581 ccggccccgg ccggtccgaa cggccacgat gtgcgggtgg gcgtcgatca agtcgcggat
     2641 gaagtggatg tcgccctggg ccaggatttt cccctggtgg atgagaatga tggaccgggt
     2701 cagggcttcg atctcgggca ggacgtggct cgagacaagg accgtccgcc cttccgtccg
     2761 gtattccttg atgaggcgga tgactttgcg cttgctcaag ggatcgaggc cgttgagcgg
     2821 ctcgtccaag atgagaatct cgggctcatg ggccagcgct tggctgattt tcagccgctg
     2881 gcgcatcccc cggctgtagc cgcggatgag ccggtcggcg tcggcctgaa gctcgacgaa
     2941 ggccaaggcc cgggcggtcc gttcccggat ctcggccgct ggaaacccgc ccagggccag
     3001 gagtccggac aggaactccc ggccggtcgt gtcctcatag aaggcatcct gttccgggca
     3061 gaagccgacg ctcttgaaga tccccggatt gttccaaacg ggctgtccgg cgatcgtcac
     3121 cgttccgatg ctcggccgga gctggccggt gatcagcttc atgaacgtcg atttcccggc
     3181 tccgttcggg ccgagaagcc cggtgatgcc gggcccgatc tcgatcgaga cgtcggacag
     3241 gccgagcacg ttgccgtacc acttgccgag cttgtccgcc cggaggatcc gcccgccggc
     3301 cgtcaccgga tcacctccgt cccccggatc ttcctccgga ggacgagaac ggccgcggcc
     3361 atgatcgcgg ccaggatcaa aatcgaataa atcccgggga cctccatcgg gggcttggct
     3421 cccaggaagg acgccgaagc ctgccggaga ttcctctgga gggaaacgag acagaaggcc
     3481 ggttcccgga agatcgcgta gaatattccg aaaaagacat cggagagaaa atacgcggcc
     3541 aggatcagga tggaggcgaa gcggcggttc cggccgagcg aggacagcag gagggtaaac
     3601 agagaaaaga aagcggagac gaacaccgac caggccagga aggaaaacgg cagccagggg
     3661 aaatcggaca ggatcttggc gctgccggaa aaaatcagct tcagcagaag gaagacgatt
     3721 ccgggggcga gggtcagcag caggagaaaa aaggccaggg cggcgccttt cccgccgata
     3781 taatcccgct ggcggagcgg ccgggaaaaa tagagcggca gggcgttgtg gcggagatca
     3841 tcggcgatca agccggcccc gcctccgacc atgatcatca gcatcatgaa gaagagaaag
     3901 ctcgacccgt aatacgcctg gaaataatcg ggattgatcg tgaggaactt atccgccccc
     3961 ttcagcatct cctggaagtc cccgaggcgc tcggaaatat agatcccgcc ggagaaaacg
     4021 accgccggca ggagagagaa gaagaagagc ggcttgaaga acctgcgctt ccaggccaga
     4081 cggacggcct ggccggcgat cacccgccac gggcgccgcc cggttccaag cgttccctcc
     4141 cagtgggtat atccgcgttc ggcgataggt tccatcaatc cactcccacc gttcgggcga
     4201 agaggtcttc cagcgacgtc cggctcttga cgaagtgccg gacctggacg ccggcggccg
     4261 cggccaagcg gaacacgtcc cgccggctca tctcctccgg caggtacgcc ttgaggagcc
     4321 cgtcctcggt ttcctcgatc cggcagccgc cgcggcggag gccctcgaga aacgaaccct
     4381 tctccccctt cactttcatt tcatacaggc tgaaccggac ttcgcgcagg gaagccagat
     4441 cgccctgggc cgcgacccgc cccttgttga ggatgaccag ggcgctgcac gtttgctcga
     4501 tgtccgtgag aatgtgcgag gaaatcagga cgtgcaagtc ttttttggaa gtgatgtcgg
     4561 aaatcagctc caggatctcg ctccggccct gaggatcgag gttggccgtc ggctcgtcga
     4621 gaaacaggat cttgggatcg tggacgagag cctgggccag cttgagtcgc tgcttcattc
     4681 cggccgaata cgtttccagg aggcggtagc gcgactcccc caggccgacg taatagagaa
     4741 cgtcgtgggc ccgtttcatc gcctcggccc ggggcatgcc ggacagctcg ccgaagttgg
     4801 ccgtgaagga gacggcatcc aggccgggga tgaaacaatc gtcctcgggc atgtagccga
     4861 cgacccggcg gaggccgaag gagtccttgc ggatgtcatg gcccaggacc agcccttccc
     4921 cggcctcggg gcggagaaaa cccaacaaca ctttcaagag ggtgcttttc ccggctccgt
     4981 tgggtccgag caagccgcag gcgccttcac cgatttggag agtgacgccg tccaaggccc
     5041 ggatcggacc gtaactgaac cggacgtccc tcagctcgat attcatgtct ctctatacga
     5101 aataaaaatg atttcgttcc cgccccctgc ttcatggatg cggcggaacc gcccggccgt
     5161 cgccgaaaac catggcccgg taggctttct cgatccggtc ccagttttcc tcgcgatcga
     5221 gcgaccagta gcgcccgagc accgtgacca ccctccccag gccgagctcg cggcatttgt
     5281 cctcgacctt ttcgacatag agggcgccgc tttcgggccg ctcgccccgg cggcccagaa
     5341 agccgtggac gtagacgtcc cgcaggcctt ggcggcgggc ggcctccagg agggcgaaga
     5401 gatggcggat cgtcccgtgc gagctgtagt gggagatgat gcccatcaag tggagggctt
     5461 tcccctttgt ccgggcttcc gtcatcgcct cgacgaacgc ctcgttccgg taaaacgttc
     5521 cgtcggcgat ggcccgatcg atccggaccc ggtcgagaaa cacgcgccgt ccggccccga
     5581 tatggagatg gccggcttcc gaatttccga ccgtcccggg cggcattccg accgcttccc
     5641 ccgaggcctg aagctccgtc cggggacagg cgtcccacag ttcgtcgaaa tggggagtgg
     5701 ccgcggccgc gatgaggttg ccctcgccgt ccggggccaa gccccagccg tcgagaatca
     5761 ggaggatgat tttccgccgc gggggaacga cgtccccccg ggaggcgaag aggctctcgc
     5821 cggtcatctc ggccggcttg ggcaggccga gaagatccaa aacggtcggg gccacgtcgg
     5881 ccagctcgcc gccgtcccgg agcgtcaccc cggcgacggc cggctcggcg aaatcaatga
     5941 aaaggaaggg aacggggctc ttggtgtgac cggtgtcgat cttgccgtcc gggtagagcc
     6001 agttctccac cgttccgtga tcggctgtga cgagaagagt cgccttattc acccgggcgg
     6061 cccggaccac ccggccgacg gcggcgtcga cgctttccac ggccttgagg atggcctccc
     6121 ggttttcgat gtgcccgacg acgtcgacat tggcgaaatt agcgacgacg agggcgtgtt
     6181 tcggatcggc cgcggcggcc tcgaccgcgg cggcgacggc gccggccgac atttccggaa
     6241 cggcgtcata gtaggccacg ccttcgggcg aaggaatgac gattcggtct tccccgggaa
     6301 acacgtcctc gctcttgccg ttgaggaaga atccgacgtg gatcgctttt tcagattcgg
     6361 agatcttgag gagggacagc cccgcccggg tgacggcctc ggacaacgtg ttgcgaaggc
     6421 ggccctcggg cgggaacgcg acccggaccg gaaggccggg ggcgtactcg atcagcgtcg
     6481 tgtaatggag ccgcagatcg ccggccgtcg gaaaatggga aaagccgggc tcgatgaggc
     6541 tccgggtgat ttcgacttcc cgctctccgc ggatgtcgta aaaaatcaag gagtcgccgg
     6601 agccgatccg cccggccgga cggccggacg agtcggtcgc gatcaacgga agaagggctt
     6661 cgtcctcttc gccggaccgg taggcccggc ggatggcctc gcccagagct gttcggggat
     6721 aggattcttt ttcattcatg gcgatggagg accgttcctg gtgaatgggg agtgtcatac
     6781 tataggaggg ggggcgtcaa agtcaactgg agcgcgggaa cgatcatcct gtcggttccc
     6841 aagaatcgaa tcgatcgtcg gcgctccgca aaaagatttt ttcccttgag tcgaacggag
     6901 tcgatttgtt attctatgtt cagatgatga aagagagagt cggcgacggc gtctccgccg
     6961 ggccgaagaa gccgccggag gaacagcgcg ctcatggata atccgcggcg cgacctgccc
     7021 gtccttcttc tccacaacct ggatccggct tggaccgggg aggaacgaag cgaagccgaa
     7081 gaggatgtcg ccgcgctcgg cgacgccttg aaatctttgg gccatcccgt cgaagtcgcg
     7141 gcggtcgagg acgcgcgcct ggccaagcgg ctggcggcct ttgatccccg ggagcacatc
     7201 gtcttcaact ggtgcgagga gctccccggg gtcccccaca gcgagccgga ggtggccgcg
     7261 gtcatggagc ggctcggttt tgttttcacc ggatcgtcct gcagcgtgct gaccctttcc
     7321 cacgacaagc cgcgggtcaa gcggctgctc gacgcggccc gccttcccac gccgccctgg
     7381 gccgtcttcc acactcccaa ggccggggat tggaatatct ttccggcgat cgtcaaggcc
     7441 gcccacgagc attgcagcat cggcatctcc cccgactcgg tcgtcacttc gccggccgag
     7501 ctcgaacacc gcgtcgccta cgtcctggag acgcacaaac aggcggccct ggtcgaggaa
     7561 ttcatcaacg gccgggaatt ccacggggcg gcttggggca atggaacggt gtccatgctt
     7621 ccgatcgcgg aaatggatta cgggaacctg atcgacatcc gcgaccgtct cttcacctac
     7681 gaggccaagt acgtcccggg atcgcgctta tacgaaggaa tcgatctgcg cgtgccggcc
     7741 gacctggatg aggacgccag ggccgagctc gagcggatcg tcttggccac ctaccgggtc
     7801 acgggctgcc gggactacgg ccggatcgat gtccgttatc tcgacggcgt attccaggtc
     7861 atcgacgtca atcccaatcc cgacatcaat cctcagacct cgatgacctg ggccgcggcc
     7921 gaaatcggat tctcctacgg agaaatgggc agccggatcg tcaacctggc ggccgcccgc
     7981 catcccgtct tccgccggaa cgactccgac gcgccggtcg ccgaataacg aggggaccta
     8041 ttccccgggc ggcgggccgt catcctcgaa tttttcgatc tccgcttctt tcttcagttt
     8101 gtccagacga agtttcaaaa gggccgcttg cgtttcgagc tcccccaggg acagctttcg
     8161 gagcgcatcc ataatgtccc tgatctcctt cagcgaggcg gcggcgcttt ccctgatctc
     8221 ctcgaaggcc gcggccgttc cggcggcggc cgcgaccgga atcgaggcgg ccgacttgga
     8281 caggccgacc acccggcctt gggcgttgac ggtcaactgc atgttccggg aggaaaaggc
     8341 ttttcgttca aacccggcgc agaggacggg agtttgagga tggacggcga agacaagctt
     8401 ttcgtccaac cggacgaaca ccggccggaa ccggccggcc tcgcggtcga ccgcctccct
     8461 ccaggaatag atccgtaaaa atccgggaac cggctcccgg taaaaaatcc gaatttcgtt
     8521 cttcccggga ggagaggggt cggcggccgg tcgatccagg atctccggtc gagccggatc
     8581 cggatcgaag gccacggcga tccccgtttc ccggtagaga tccagcaccc ggggatacgc
     8641 cttttccaaa gcgtcggtga ttcccgcctc atcgaaaacc ccggtcaagg ccgccgacgg
     8701 cggcaggtcc gtgatgtcca agtggcggcg gaccttccgt tcccgggtcg tccggccgat
     8761 tccctcggcc gccgaaaatt tcacggccgc cgaggcgaaa gccgcccgag ccgcctcggt
     8821 ctgcttttct aaaaaccgga tcgatccttc gagaaaggcg gcccgcttct cggcgaggga
     8881 ataacgagcg ggctccgttg tttccaccgc ctcgtccctc acccggcgga gggcttcttt
     8941 tcgtccggcc agctcgttct cgaggcggtc cgcctcctcc cagagctccc gccccaaaga
     9001 cgattcctga agataatacc ggttctcgag ggacaacgac tcgaggcgcc gccgggctcc
     9061 ggctccggaa gcgtccatct ttttcgtccc cgcgttcagc cctccccgga aaccggccgc
     9121 cgttccggcg gccgcgccga ccagccgggc gatctgaacg aggatttccg ccgacttgtc
     9181 cttagccgcg aactcggcgg aggtcaaaag gccgttgttg cccacgcgga cggccaagga
     9241 ttgatcggcc gcgccgccaa gggcggcgtc gagcatatag gggcgggacg gatcggccga
     9301 tgtctcgagg ccgaccgagg ctttcaccgc gaaaattccg gattccgtca ccggcttcaa
     9361 ccatccgtcc gctccggctt cgattctcgt ctcgacgaat tcggttacgt ccgcctcgac
     9421 gacgacaaaa tcccgggcga gataatatct caatcccccc agaggcttcg gcgccgtcga
     9481 cggccctcgg cccgccgggg cgcagcccat catcaacccc caaaccgccg cccccagcat
     9541 catccaccct gcccgcatga tttataaaaa ttcttttgat tcatatcttc cttttggcgt
     9601 caaaaagaaa ttcaccatga gctttttcct ttcgcgcccc gcgcccgccg tccgccctcc
     9661 tcgtcaaaaa caccggttaa gagctttccg acgcccccgg cctcccgggc cggccgcgcc
     9721 cgcctcgtcc ttgattttcc catttccggc aaaggcttat tccgtgaaaa ccgtcgcccg
     9781 cggcgattcc tctcccagcc aaaacggctt ttcgccggcg aattcctccg cccccggtcc
     9841 gcaaagcatc agccgcggat aaggcggatc aagattcctc tcgatccggc ggaccaggtc
     9901 ccggtaatcg cccggcgaat ccgatgtcaa cgcttcgcac aaagccaggg tgaacgcccc
     9961 tccgccccgg taagcgggag accgctcccc gtccggaacc gcgccgatat gaatgaggga
    10021 agctttcatc ggaaggtggg ccggacggaa ggcgggccgc ggagaaacgc gtcttcggcc
    10081 cccgaagttt ttatagaccg tgccggaatg gcaggcgtcg atgatcacga ggatccgaac
    10141 gccgccggcg aactcccgcc agcaggcgtc caattcgtcg tcggccaccg gacggtcgta
    10201 agccagcagg agctcatcga ggccgtcttc ctcttcgccg ccggaatcgg ggcgctgtcc
    10261 cccgtggccg gcgaaataaa aaaacaggaa atccccgggc cggcacagcc gggcggcccg
    10321 gcggagattt cccaacagcg cggccgcggt cgcttcggcc gtcttcaagg cgaggcactc
    10381 gaagccgcgg gaggagataa agcggatgat ccggtcgacg tcttcttccg ccccccaaca
    10441 gcccgaagca gggacggagc cgccgtaaga cgacgggtcg acgcgcctca atccggcggc
    10501 gaccgccaag ccttgagcca tgttgacctc cgcttttttc ccttcagtcg gaacggggcg
    10561 aaaaaaatgg aattatggat tataatttcc tctttgcgac gaccgaaacg gaacaaggag
    10621 gccctcaggt gaaaaaacga attctcgtcc ttctcttcgc cgccggattc ctggccgcgg
    10681 cgctctcggc ccaggctttg aagccgctgt ccatcgacga cttcctcaag atcaagcgct
    10741 tgaccgaccc tcagttgtcc ccctgcggaa aatgggtcgc ctacacggtc acggtcatgg
    10801 acaaggaagc gaacaagggt gacagcgata tctgggtcgc gccgctcgcc ggaggcgacc
    10861 cgcgccttct agcctcgagc ccggccgcgg atcacacgcc ccgctggtcg cccgacggca
    10921 agcggatcgc cttcatctcc tcccgcggcg gaacgcccca gatctggacg gtcgccgccg
    10981 agggcggaga gccggtccag ctcacgcgac tttccaccgg cgcgtccggc gtcatttggt
    11041 cgcccgacgg ccggctcctg gccttcgcct cgtcggttta tcccgaggcg gccgnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgaggc ggccgccgcg ggcaaggtca
    11221 aagcccggct gttcgacacg ctcctcgtcc gccactggaa cgcttggagc gacggcacgc
    11281 ggagccacgt cttcgtcgtt ccggccgccg gcggcccggc cgtcgacctc acgccggggg
    11341 acttcgacac gccgccgatc gccctcggcg gaagccagga ctacgtcttt tcccccgacg
    11401 ggaaagagat cgccttcgtc cgcaatatcg atcccgaatt gaaaaaaggg ctggggacga
    11461 acaacgacgt tttcgtcgtc ccgtccgccg gcggagagat ccgccggatc acgacgaaca
    11521 aggccaacga ccattcgccg ctctactccc ccgacggccg atacctggcc tacctggcca
    11581 tggcccggcc gggattcgag gcggacaagc agtccttgat gatctacgac cggacggcgg
    11641 gcacgacggt caacctgacc gaagctctgg accggtcggt cggcgagatg ctctggacgc
    11701 cggactcggc cgcgttgtat ttcgtctgcg acgattgggg ccggaaagcc gtcttccggg
    11761 ccgccctgga cggccggaag gtcgaacggg tcctggaagg acacaatctg tcctcgctcc
    11821 aactgacgcc ggatggaacg acgttcgtct tcgcccggca ggcgatgaac cagccggccg
    11881 atgtcttcac ctgccgcttg gccgataaaa cgctgtcccg ggtcaccgat atcaaccggg
    11941 atcttttagc cgggctggat atgaacccgg ccgaggaaat cacctttctc ggggccggag
    12001 acgagccggt tcacggctgg ctgctcaagc cgcccgcctt cgacgcttcc aagaaatacc
    12061 ccttggtgat gctcatccat ggcggtcccc agggcgcctg gacggacgaa ttccatttcc
    12121 gctggaacgc ccagctgttc gccgccccgg gctacgtcgt ggccatgatc aacttccacg
    12181 ggagcacggg ctacggccag gccttcaccg attcgataag cggcgattgg ggcggcaagc
    12241 cctacacgga catcgccaag ggcatgggct acatccacgg ccggtatccg ttcatcgaca
    12301 tcagccggaa cgcggccgcc ggcggctcct acggcggcta catgatcaac tggatcgaaa
    12361 gccgcgggac gaaggtgttc aattgccttg tcagccattc gggcgtcttc gatctgcggt
    12421 ccatgtacgg agcgaccgag gagctgtggt tcccggaatg ggaatacctg ggaacgcctt
    12481 ggacaagccc cgaacaatac cggaagttct ctccgtccag caccgtcgct gacttcaaaa
    12541 cgccctgcct cgtcatccac ggggccaaag atttccgggt ccccctggaa caggggctgc
    12601 agatgtacac gtccctccaa cggatgaacg tcccgtccaa gttcctgtac ttccccgacg
    12661 aggatcactt catcctgaag ccgcagaact cggaattgtg gtaccggacg atctgggagt
    12721 ggttcgccga atacctgaaa tgagccgggg gcttacgacc ggacggggcc gcgccgggag
    12781 aggatgaaaa gcgttccgtt tccgcccagg tagccggcgg tcagggcgat gccggcggcc
    12841 gtcgccccga tcattccgag gggcccgacg gtcaaccaga aaaccgccgc gaccatcgcg
    12901 atctggacca ggacggcggc cgcgatcggg ttggtgtccc gggcccggac gaggatcccc
    12961 cgccggtagg cgaggaccgc ctccagaaag ggaaggagga cgagcaatcg ggcgggcagg
    13021 agggcgaatc cggccagttc cggcgacaag cccgaaaccg ttcccatcca gaccggggcc
    13081 aagggcgaga ataccaccgc ggccatcccc agcgtcccgg cgagcccgat cgtcccggcc
    13141 atccggcgga ggaggatctt gtgctcgccc cggtcgccgg ccagggcgat cacgacttcc
    13201 tggacggcga tgccgcccgt ccggaagatg aaggccagcc ccatgatcac cggcaccacg
    13261 gccagcgatt cgatcggcat ccggctccgg ccgaggaaaa acgacgtcag ggggtggacg
    13321 gaaaaggaaa gaaacgaggt catggccagg gggacataaa accggccgat tttatcgagc
    13381 gtcagagccc ggccgaaggc gcactcctcg tcgctttggc ttcggagccg ccggaccaat
    13441 cccctggcca taaaccggct ggccgccgcc tcggccagaa ccccgatccc caaagccccc
    13501 ccgccgacgc aggcccccgg gaggcggacg gccagggcca agacgacccc cgttgcggcc
    13561 atggtcgaaa ggcggaccgc cgtcccccag gccacgcccc gcggcttccc cccgcggatg
    13621 agaatcccct ggtagaaccg gcgatagccg atcgccgccg gccagaggat cagaaagatc
    13681 atcgattggg ccgacagccg ggcgacgtcc tcgggcaggc cgatcactcc ccgggcgatc
    13741 caccggaaaa ccggcggaag aagaaggagg atcatgaccg cggtcacggc cgcgttcagg
    13801 acggtggaaa agcggcggag gcgccggaag gcgattttat cctggactaa ggcgttggaa
    13861 gccgaaagca tcatgacgat gggcgactcg acgatccagg ccagcgacgt ggccacgccg
    13921 aaggcggcca ggttgatctt gggatcggcc aagcgggcga tgatcgcggc cacgaagggg
    13981 ccctcgacgg ccatcatcag ccaagtcgcc tggagcggca gccaatagtt cagggcggtc
    14041 cgggacgtca gggcggccgc cggtttttga ttcgggctca tggggcggga ggatatcatg
    14101 atcgggacgc cggcgacaaa cgcggaatcc gcgcgattgc cgcccggcgg ccttgccgaa
    14161 catcataacc gggatgaaag cttccgatga ccggtttccc gcccggatta gaagtccggt
    14221 tccgtcgtga aaggagtcga ccttcatcat ccctccgctt gacaagccgc ccgcggcggg
    14281 gtatatagga aacgaatgtc gattatttag ttttcttggt ttccggaggg accatgaccg
    14341 gcgaggcgcg ggaccggatc gtcgccgaag cccggcggct tttttaccag ttcggcttcc
    14401 ggcggatcgc catggacgaa atcgccgcga acctccggat gagcaagaaa acgctctatg
    14461 ccctctttcc ctccaagaag gagttgatcc aggcggtcgt cgaatccatc ctggaaccga
    14521 acctggcccg ggtctccgcc ctggcccgga acgaaaaatc agtcgccggt tttttttccg
    14581 gaagcatcgc cgtcttccac gggctgtctc ggtccatctc cgagccgatg atggccgaca
    14641 tgcggacaat gcccgacgtc tggagcaaag tggaagcccg ccgtcgcgag gcgctgatgg
    14701 ggctgcggca ggttctcgaa agaggcaaac ggtccgggga aatccggcct gacctccacg
    14761 tcgatctctt cctccggatc tatctcctga tgatcaacgc cgtcggaaat ccggcggtga
    14821 tcctggaact caacatgaag ccgtcggagc tggccggcca gctcggcggg cttttcctca
    14881 aggggattct caatgacaag cggcggaccg gaggtgcgtc atgaaacgcg gactcgggct
    14941 cggcgttttg atcgtcctgt gcgcctgcgg cggacggagc gtccggaacg agatcatcct
    15001 gacgggttcg gtcgaaagcg atccggtgcg cgtctccccg ctcgtcgggg gacgcctgct
    15061 cgaagtcgca gccgtcgagg ggacccgggt cggcgcgggc gggatcatcg cccgaatcga
    15121 cgcgaccgac ctcgagcttc agctccggca agccgaaacc gccgtccgga cggccgaaac
    15181 ccagctcgcc ctcgtccgga agggcgttcg gagcgaggac ctggccacgg cccgggagca
    15241 ggtcaggcag gcggaaatcg cggtcgacaa agccgagcgg gagaggcggc gcctggcccg
    15301 gctgtacgag gaagggtcga tcccggccaa ggcgctcgat gacgccgccg tccaggccga
    15361 ttcggcccaa agcgtcctcg aacaggcccg aaagcagtac gaaaaagccg aagccggcgc
    15421 ccggaaagag gaaatcgagg cggccgaagc tccccgcgac caagcccggg ccgcggccga
    15481 catcctgagg aagaagatcg gcgattgcct ggtcctctcc cccgtcgccg ggaccgtcct
    15541 ccaccgcttg gccgagccgg gggaagtcgt cgctcccgga acaaccctgg cgatcgtggc
    15601 cgatctttcg accgtcaaga tcacggcgta cgtcccggaa cgggacttgg gctccatcgc
    15661 cctgggaatg agcgcctccc tgcgggcgga cgccttcccc ggccggagtt ttccggcctc
    15721 ggtttcacgc atcgccgacg aggcggaatt cacgcccaag acgatccaaa cgcgggacga
    15781 gcgggtcaaa accgtttacc ggatcgagct gacggccccg aatcccgagg gggtcttcaa
    15841 gcccgggatg ccggtcgacg tcgtcctggc ccggcccgaa agccggccgg cggcccgctg
    15901 agggagcggg gataacatgg ccgaagaggc agccctccaa gcatccagcc tccgcttctc
    15961 cttcgggcag acggaggcgc tcggcggcgt cgatttggag gtcgcccggg gggagatgtt
    16021 cggcctcgtc gggccggacg gggcgggcaa gacgaccctc atccgcgcct tttgcggcct
    16081 cctcgccccg gactccggcg cctgccgcgt tctcggcttg gacacggtcc gggaccgggc
    16141 ccggctcatc cgccggatcg gctacctctc ccagcgcttc tccctgtacg gcgacctcag
    16201 cgtcggggaa aacctcgact tcttcgccga aatccacggc cggaaagatt ccgccggccg
    16261 caaacgccgg ctgcttgagg agatggacct ccttcgcttc cggaagcgtc cggccgagaa
    16321 tctttccggg ggcatgaagc agaagctggc cctggcctgc acgctcattc acgcgcccga
    16381 gatccttttc ctggacgagc cgaccaacgg cgtcgatccc gtcgcccgcc gcgatttctg
    16441 gaacatcctg gccggtcttt cggccggggg ggtgaccgtc gtcatttcca cgccctacct
    16501 ggacgaagcc gagcactgcg gccgcgtggc cttcatggcc ggggggcgga tcgcggccgt
    16561 ggagacgccg gccggggcga aggagcttgt cagggagcgg atctgcgaaa tcgtctgcgc
    16621 ccccgtcttc cgggcccgcg aggccgccgg cctcgtcccc ggcgtcgttt cggtccatgc
    16681 ctacggggac aagatccgcg ccgccctcgc ggccggggcc gaacccgacg ccctccgggc
    16741 ggccgtggag aaaaacgccc ggatctcggc cttccggatg gtccggccgt cgctggagga
    16801 cgtcttcatc catttgctcc gccggaacag ggggacgacg gcatgaccgc cgctccagcc
    16861 gtggaagtcc gcggactcga gaagcggttc ggccgcttcc gggcggtcaa ggcgatcgac
    16921 ttttcgatcg gccgggggga aatcttcgga ttcatcggag ccaacggggc gggcaaaaca
    16981 accacgatca agatgctctg cggacttctc ccgcccgacg gcgggcgggc cgtcgtcgcc
    17041 gggatcgacg tcgccgccga cccggagggc gtcaagcggc ggatcggcta catgtgccag
    17101 aagttcgccc tgtacggcga cctgaccgca accgagaacg tcgatttttt cggaggcatc
    17161 tacggccttc ctccggagcg gctccgggcc cggcgggccg ggctcttccg cctcctcgac
    17221 ttggaaagcc gggccgggcg gcgggcggcc gacctgccgc gcggttttca acaacggctg
    17281 gctctggcct gcgccgtcgt ccacgaaccg gccgtcattt tcctggatga gccgacggcc
    17341 ggggtcgacc cggtccagcg ccgggcgttt tgggatctga tctacggctt cgcctccgcc
    17401 ggaaccgccg tcttcgtcac cacccatttt ctcgacgagg ccgagtactg ccaccgggtc
    17461 ggcttcatcg ccgaaggcga gctggccgcc gtggacaccc cggccgagtt gaagggccgg
    17521 ctgaccggct ggacaatcta cgagctgcgg acggccgatc cggccggaac gctgtcggca
    17581 atccaagccc tccccggcgt ccgttcggcc gcgctgttcg gcgagacgat ccacgtcctg
    17641 gcgtccgaag ccgccgatcc ggccgatctc ttcggccggc tgtccggacc cgccggatac
    17701 gacccgatcc cgccgactct cgaggacgtc ttccttcacc tcgcggggaa aacgccgtga
    17761 agctcgccgc ggtcgtcaaa aaggaattcc tccaaatccg ccgcgatccc ctgaccctgt
    17821 tcatgctcgt tttcttcccg acggcgctcc tcctgttttt cggctatatc ctgtccttcg
    17881 acgtccggaa cgtccgcctg ggggtgctcg acctggacgg ctccgattct tcccgggcat
    17941 tcatccggca attgacgggc ggcgggtatt tccagctgga aacgatgttc gccggacgcg
    18001 gggaagccga cacggccttg gcccgcggct ccgtcatggc cgccgtggtc gtcccgaaag
    18061 gattcgggga agccctgagc ggaggcaaaa cagccgccgt ccaggggctg atcgacggct
    18121 ccgacagccg gaaggccgcg atcgttcagg gctatctcca ggcctacgca accgccttca
    18181 gccggcaggc gctcggcgac tgggccgcgc ggctcggccg ccgtcccgtc gtcccggtcg
    18241 aggtcgaggg acgcgtctgg tacaatcccg agctcaagtc cagcctccat ctcctggccg
    18301 gcctcatcgt gttcatcttc atgatcacgg gaacgatatc gacctcgctg tccgtagtcc
    18361 gggagcggga gcgggggacg atggagcagc tcctcgtctc gccgcttggc gccgggaccg
    18421 tcatcctcgg caagaccctc ccctacctcg ccgtctcggc cgtcagcacg gtgatcattc
    18481 tgatcgtcag ccgcttcgcc ttcgggcttg cggtcaaggg atcgatcgcc ctgctcgccc
    18541 tggcttcgct cctttttctc cttgcggccc tcggctgggg catcctgatt tcgaccgtca
    18601 cccggtccca gcaggtcgct tatttcgcgg ccgccctttc gagcattctt cccgctcttc
    18661 ttctttcgga tttcgtcttt cccatctccg ggatgccggc cgtgatccgg gcgatcacgg
    18721 cggtcgttcc ggccaagtac ttcgtccgct tgcttcgggg catcatgatg cggggggcgg
    18781 gattcgccgc cggctgggcg gacttaggcg ccctagcgct tttttcagcc ttcaccttga
    18841 cgctggcggc cgttcgtttg aaaaaggtca agctggcctg aggcggacat gctcggaaaa
    18901 atcatccaca aggaactgct tcagctgagg cgggacaagc ggatgttccc catcttgatc
    18961 gccgcccccg ttctccagct tctcattctc ggctatgcgg ccaacttcga catcgccgat
    19021 atgccggcgg ccgtctgcga cctggaccga acgccggaaa gccgcggctt cctgaacggc
    19081 tttttcggca gcggctattt cctccgggcg ctcgacgtcc cgggcggggc cgatctcgat
    19141 cccgtcctgg aagcgggccg ggcccaaatc ggcctcgtca tcccgcgggg cttcggccgc
    19201 gccctcggcc ggggcgaggg gacgaagatc caagtcatcg tcaacggttc ggaccccaat
    19261 tccggcatca acggatacgc cttcgccctc caggcgacgg cccgctattc gacggccgtc
    19321 ctgatcgagc ggatggccgc ctccggagtc gcctcgatcc cggcggttga tctccggacc
    19381 cggatctggt tcaacccgga gctgaaaacc cgctatttca tggtcccggc cctgtccggg
    19441 ctcattctca tgatcatctc catcatgctc acggccatga cgctcgtccg ggaaaaggaa
    19501 accggaacca tcgagatgct ggtggtgacc cccgtcaaga agctgtccat gctggccggg
    19561 aagctcctcc cctacctggt catcgggctg gtcgaggtgg gtctcatcgc cctcgtggcc
    19621 gtcttctggt tcgaggtccc tttccggggc tcggctggac tcctcgtcct gggatcgctg
    19681 ctctttcttt tcaacaccct cggcctcggc ctgttggtct ccgccgtctc ccggacccag
    19741 cagcgggcga tgatgatcgt catgttcgcc gtcatgatgc cgttcatcta cctgtcggga
    19801 ttcatctttc cgatcgaggc catgccggcg gccttccaag cggtctcccg gttcattccc
    19861 ctgacccatt acctggagat cgtccgcggc ctttttctca aaggatccgg gttcgccgac
    19921 ctggccgggg ccctggccgc cctggccgga tcggggacag tcattttcgc cctgtccgtt
    19981 ctccggttcc ggaaattcct ctgaccttat ttcagaaagg gaagcaggcg ctcgagggga
    20041 agcgcctcga ccttgcggcc gccgcatccc tccatggttt ccgcttggaa aagggaattg
    20101 agaacggcct cctcggccgc ttcggccgcc gccaggaaaa gaggcgtcat ttcatcgtcg
    20161 cgcaattccc ctccagagag gacggccgag ccggattcgt ggggaatccg catgtccggg
    20221 ctcgtggaaa aagcgatcac gtaatcgccg cttccgttgg agccgacgga tccggtgcgg
    20281 gccagtccga aaacggctct tttggccagc cgctccaggt tgcgggcccg gaggggcgcg
    20341 tcggtcgcga ccacgatcat gcacgaaccg tcggcccgcg acccggccaa ttcatccttc
    20401 aagtaatgcc ggtccaggag gattccggcc ggctccccgg cgacttgcag aacgccgccg
    20461 tagttggtct ggacgagcac gccgacggtg tagccgccca gcttcgccgg cagcgcccgc
    20521 gacgaggttc cgatcccccc cttgaagccg aagcagacgg tgccggttcc ggctccgacg
    20581 ttgccttcgg ccaccggacc cgaggcggcg ctccgcaggg cctggagaac gtgggccggg
    20641 cggaccgggc ggcggcggat gtcgttcaga aagccgtcgt tggtttcgcc ggccacggcg
    20701 ttgaccgagc ggacgttctc gttgcccggc tgggcgagcg tgtattcgat caatccttcc
    20761 acggcctggg aaacgtttaa agtgttggtc aggacgatcg gtgtttccag gctgcccagc
    20821 tcttccacct ggctttggcc ggcaagcttg ccgaaaccgt tggccacatg aacggccgcc
    20881 ggcactttgt cttggaagag attgccgccg tggggaagaa tcgccgtcac gcccgtccgc
    20941 acgtccgttc cctcgatcag ggtgacctgt ccgacttcca ctccggccac gtcggtgatg
    21001 gcgttccatt tccccggcgg cagaatccct gttttcaacc cgaaatcgcg gaaacggccg
    21061 cgccggccgg ccgcggccgc cggagccgct tcgagctccc ggaaagtccg gacggccgcc
    21121 cgggccagcg cgagcgtggc gttgacgacc ggaaccgtaa cgcacgtttc gtcgaaagcc
    21181 aaggggattt cggtgcatcc gaggacgatg atctcggccc cccgggcggc catctccctg
    21241 gccggctcgg ccaagagagc ggcgttctcc ggtttcggcc gcccggcctt gatgccggcg
    21301 accgcccgca tgatccgctc ttcctgagcg ctctccgggg gaacgagcgt ctccacgccc
    21361 ctggcccgga attccttttc atacaatccg gtccgcagag tgccgctggt ggccagaaga
    21421 ccgacctttt tgacgtccgg ccgaaggcgc aggacttcgt cgacggcttc ccggatcatg
    21481 tgaagcaccg ggatcttcac cgccgcctgc atctgatcat gatagaaatg ggcggtgttg
    21541 cagggtatgg cgatgacgga ggccccggct ttttccagga actgaacccc ctggaccaga
    21601 taaggaatga tggacggatc attgttcaaa atgcaggtgg tccggtccgg gacctgggga
    21661 aagctgaaca tcagcgtcgg gatgtggtcc tggtcctttt gagccggcgt ctgctgaacg
    21721 atgagctggt agagattggc cgtggcttcc ggccccatcc ccccgaaaat gcccagcagg
    21781 cgggaaggaa gaacgccccc gtcccgctcc gcggccgccg ccggcggaac ccgcgccgcc
    21841 cagaaggcca ggacaatcag tccgatgatc aagcgccatg acggttttct catctccgat
    21901 cctccttgat tcgaatcgtc cgtttctttc gattcatccc gcccgatttg cggagcgggg
    21961 cggctgaact cccttcattt cattaaaaag aaataatgaa agaagcaaaa gaagcaacgg
    22021 cgtttcatga atgatgacgg ttatctccgg cccggtcaag ggcttttcat tccaggggaa
    22081 gggcggccag gctgaaggga tcgacccggc tgtcgagcac ccggacgctc caatggagat
    22141 gcgggccggt cgagcggccg gtactgccga ccttggcgat cacctcgcct tttttcaccg
    22201 gctgccctct tttgaccagg atcttcgaga aatggccgta atacgtgaaa acgccgaggc
    22261 cgtgatcgat gatgaccgtg ttcccggaat aatagaggtt ggcggcgagg acgacccggc
    22321 cggaattggc cgcccgggcc ggcgttccgg cgggcgcgga aatatccacg cccgaatgga
    22381 tggaggcgac ggcgttgttg taaagccggc gttggccgaa gttggggaag ggctccccct
    22441 ccggcaaggg cgtcgcgaac gaacctgttc ccagccattc aggcgagacg acgcttaaaa
    22501 cggaggcgac gagggcggcc tcgcgctcga tacgctcggc ctcctccggc ggagccttgg
    22561 ccatgcccgg agcgacggtc agccgggtcg agggaaactc cttcgagctg accgacagct
    22621 tccggaagaa cgactccctc gatccgtccc gccgttcgac cgtaaactcc aggaaatagg
    22681 cttgagcccg ggccgaaaga tcgatgccca ggagaatcgt ccgtctcttt ccgtcaaatt
    22741 cgactcgtct tttttgagtc cggaaccgaa gatcgatccg gcggatcgac ggatcggcca
    22801 gcggcttcac caggaccgct tcccccgggg ccagcgcccg gaaggaaaac tccagcggga
    22861 tgccgggaag gacctttatt tgaagtccgg attcttcaaa gccggcgaag gcggcgacga
    22921 ccagaatccc cagccagacc gcccggaccg ccgttttcgc cgcgcccggc cgctttcgtc
    22981 gatcgtccat gccccgctct ccccccgcct aaaaagcgac tgccagaatg accgcacccc
    23041 ggccgggacc ggcctgaaac gagacacgga gcccgtcgcc gttccggttg agggcgtcga
    23101 cggcccgggc tgtgaaatat ccgatggccg agccggcgaa cacgtccgaa ggccagtgct
    23161 tccggtcatg aacccggtaa aaagcggcca ggccggcggc cagataagcc agggagtcga
    23221 tccaaaacga atccgagttc agggcgatcg tcgtcgcgac ggcaaacgcc ccggccgcgt
    23281 cgcccgaggc gaacgaagtg tagcgtccct taaaggaaaa tggatgaaac gattccggcc
    23341 cttccccggc atacggccgg gcccggccgg cggcgatctt gaaggcgaga ttcacggccg
    23401 aggcggcgat caagctttcc agcccggtca aggcggttcg gcggagggag cgggaatcga
    23461 ggatctcccc cccgccgtat aatacggcca ggaaccccgc caaatacccg ccgttgccga
    23521 ttttcgagat gatcttcgag gcgtcgcggg aggcgtccgt tttccggtct tggatcgctt
    23581 ggtagaaatc gccgtcgaag gcgaaaagaa gcccggtcgt tccggcgaac gcggcgaacg
    23641 tcagaagatc gccgccccgc caccggaacg gagcggtccc ggcggcgagg atgtcggaag
    23701 cggctttctt gaggtaagct ccattcagcc gccaatcggg attcgcggcc gccggcccga
    23761 tgtcctcggc cggggcatac cccgcccccc aggccagagc aaggacaagc aggaaagccg
    23821 ccgggaagcg gagccgccta ggcatcgcgc tcctccagaa gggattccat cgccgccagc
    23881 gaccgtctgg tcgccccttg aagggaccgg aggatctcct tagaccggcg gcccatctcc
    23941 tccatcccgg ccggatcgtc aaaggcgaaa aaagcggcga tgtcatcctc cgtccggacg
    24001 gttttcgctc cgccgccgcc ctcgaaggcc tcggccagga cggcgaagtt tttcatgtgg
    24061 ggtccgaaaa cgatcggctt gccgtagaag gcgggctcca ggagattctg cccgccccag
    24121 ggaatcaagc tgccgccgat gaaggcggcg tcgccggccg cgtacatccg ggccagttcg
    24181 ccgatggtgt ccaggagcag aatatcccac ggccgggacg cgtcaagggc cgtttttttc
    24241 tgaatcacga atccggcgcc gggcctggac gtctcgaatc cgccgaattt atccgggtgc
    24301 cggggagcca gaatcatccg cacctcgggg cgccggagcc gggcggcccg gaatgcttcc
    24361 aggagcaggt cctcctcgcc cggatggacg gagccggcga cgacgacctt ggcatccggc
    24421 ggaatcccca ggccggtctt gacggccgcc agctcggcgg gggtcagttc gggcggacgg
    24481 gtgtcgcact tgagattccc ggccatctgg attttcgagg agggcacgcc cagcttctcc
    24541 aggcgctccc catccagcgg cgtttggacg agaaatcggg ccaccttagc caaaagaaaa
    24601 tcggccgccc acgggaaccg ccgcagccgt ccgaacgtgc gggcggagac gcggccgttg
    24661 accaccagaa ccgggcatcc ccggcggccg gcctcgcgca ggagacgggg ccagtactcc
    24721 gactcggcca ggacaagaag cgccggccgc aaacggctga agacgcggcg gacgcaccag
    24781 cccaggtcga agggaacgaa aaagatgtgg tcgacgtccc ggatccgatc gcgggccagg
    24841 gcatatccgg actgggtcag gacggaaaac ccgatttccc agccgggatg ggccttccgg
    24901 atttcctgga ccaggttctg aagcgagagg acctcgccca cggagacggc gtggatccag
    24961 acgaacggcc ggccgccccg gggaacaggc cggacgaagg ccagccgctc cttgagatgg
    25021 agcggtttct tccgccggat ccgcagacgg acgcaataaa gggggaggag gacggcgaga
    25081 gccagggcca gaagggccga atagatcgca tacacgattt caagactata ctggaatcgg
    25141 cggccttttt ccagaaggga aaggcgttcc gattacaaac tcctggcaat cccttaaaaa
    25201 ttctgctata cttcgcggga ataaaaaaca ttgtgactct aagttcaaag gaacaacgac
    25261 gatggccgaa gaaaccaagc ccgagggcga gaaaaccgcc aaacgaatca aaatcaataa
    25321 gttgagcgtc tctgaaatcg acgccaagct gaacgaagtc aaaacgtccc aaggcggcct
    25381 cggctcccgt tacgccagac agcttcttct ccgtaaaaaa gctctcggaa aataatcgcg
    25441 cttccgcgga ttccttcccg ccggtcttgt cgagtcccct ttcctccccc gtccggccca
    25501 agtttctttt tgcccccgtc tatgttagga tatcgcaaga aaataaagga gatataaagt
    25561 gttgacgaaa gaaaaaaaga cccacctcgt caccgaaaac cggatccatc cgaccgatac
    25621 gggctcggtt gaagtccagg tcgccctgct cagcgagcgc atcaacgcct tgaccaagca
    25681 catgagcgcg gccaagaaag accacagctc gcggaccggc ctgatgaaga tcgtcagcca
    25741 gcgcaagcgg ctgctgatct acctcaagcg ggaagatccc gcccgctacg agaagctgat
    25801 ccagaggctc ggtctgcgca agtaagcgcg gacgggcatg tgatgaaaat gattattccc
    25861 tcattcagtc tcgaaatcaa caaccgcacc ctgtccgtcg aattcggcaa gatgggcaaa
    25921 caggccgacg gatcggccac tgtccgctac ggcgaaacgg tgatattcgt cgccgcgacc
    25981 tccaagaaag aagcccgcga ggacaagccg ttcctgcccc tcgtcgtcga ttatcgcgag
    26041 aacacctacg ccgcggggaa aatccccggc ggattcttca agcgggaagg ccgcccctcg
    26101 gaacgggaga ttctcgtttc ccggctgatc gaccggcccg tccgctcgct gttccccgac
    26161 ggctatcaca atgaaaccca gatcgtcgcc ctcttgcttt cggccgacct cgaaaacgag
    26221 cccgacacgc tcggcctgat cggggcctcg accgccctga ccctctcgga aatccccttc
    26281 accactccgt tcggagcggt caaggtcgga ttgatcgacg gcaagtacgt catcaacccc
    26341 acggccaccg agctggaatc cagcccgctc aacctcctcg tcgtcgggat cgaggaaggc
    26401 gtctgcatga tcgaagccgg ggccaaggaa atcgacgagg aaacgatgct cggggcgatc
    26461 gaagccgccc tcgagcctat ccgccggatc atccaagccc aaaaggacct ggccgcccgg
    26521 atcggagtca agaagcgccc gttcgagccc aagcccgtcg acgaagccaa gctcgccgcg
    26581 atcgaaggca agatcggagc cgccctcaag gccgccatcc aaacgccggg caagaaagcc
    26641 cacgaggccg ccgtcgaggc ggtccagaag gagctgatcg ccggcatccc cgaggagaac
    26701 gaggacgagc gggccgaagc caagcttttc ttcgagaaaa tctacgaaaa atacgcccgc
    26761 cgcatgatcc tcgaggacgg ccgccgggtc gacggccggg gcttcgacga catccggccg
    26821 atcaccatcg acgtcggatt cttgccccga acccacggtt cggccctctt cacccgcggt
    26881 gaaacccagg ccctggccac ggtcaccctg ggcacggtcc aggacgccca gcgcttggac
    26941 ggcctgggcg aggagactca gaaacggttc atgctccact ataactttcc gcccttctcg
    27001 gtcggcgaag tgggatttct ccg
//
LOCUS       DAOOXH010000026        25257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_1763_length_25257_cov_8.704706, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000026 DAOOXH010000000
VERSION     DAOOXH010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 25257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25257
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1763_length_25257_cov_8.704706"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="PKV64_04785"
     CDS             complement(<1..782)
                     /locus_tag="PKV64_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HPN16431.1"
                     /translation="MLKRYQIVNQKIVETAEDKAAVLLYLNPDESEKRRLVDEYQIDE
                     HTLASALDPDEQSRLECEPNHIAFIFKRPKNYSGDDQLLFKASTMGAFFFGDVLILVA
                     ADDTVIFEGKQLARVTSLRELTIRLVYRAIYHFMEHLRIINMMADDVEHKINKAMENK
                     YLINLFALQKSLVYYQNAVGSNGVLIEKMRHNAGRFGFTQEEMELLDDLVIENSQCHR
                     QAEVYSNILASLMDARASIVSNNLNVLMKTLNIITISIMVPTL"
     gene            complement(883..2022)
                     /locus_tag="PKV64_04790"
     CDS             complement(883..2022)
                     /locus_tag="PKV64_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HPN16432.1"
                     /translation="MKIVVLGAGLVGAPTALDLAAEARFEVTAADRDAEALARLEGKP
                     NIRTVRVDLADPKTVTRLVENYDLVLSAVPGFMGFQTLGAVIAARKSVVDIAFFPEDP
                     FLLQEQAEAAGVTAVVDCGVAPGMSNILAGYAASRLEETQSILIYVGGLPEIREWPYE
                     YKAVFSPADVLEEYTRPARYIHNGELVVKPALSELERLEFPGVGTLEAFNTDGLRTLA
                     RTMRVPHMKEKTLRYPGHVEKMAMFRDSGFFDTDEIEVGGVRVRPRDVTARLLFSRWK
                     LREGESDLTVMKIIVEGRRLGNALRYTYDLLDRRDPATGIHSMARTTGYTASCALRLL
                     AGGLFADKGIVPPEIIGRRPECTAFLLDALRERGVVYQETVEHIS"
     gene            2252..3523
                     /locus_tag="PKV64_04795"
     CDS             2252..3523
                     /locus_tag="PKV64_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16433.1"
                     /translation="MNKSTVHLIFLWAAVLAAGSLLANAASAGGRAADERLLQYSIVG
                     SSSCDRTPCFNGVMARIPVQRFLLELAEGPMARAKAEARDLGAAGIADLLKLRLVRFD
                     GDRYLLNFALFTAADVGRIREVSERYAGSLAEALLARRADIETALRAYDVPGVDPGSV
                     AYFLLGCVSLDWDGLAVTDEKKYRKTTEERFDGSYVPAAEELGETSLEKIYWGSHYEE
                     FSGVHFTSFGDHHALPRMLFPDLLWNVPGLSSRYPEAVRNAHRELLSDSLRRGGAKIG
                     RMMLALREGPKAAGDLARAAGTDEDESRVFLRLLSVLEYVREKDGWYETRIPVLTGRD
                     AVMTAELRAIGRRVMEQWLAGNYDRLAADLDGLSFTRSGVPFSEGFTMIWHYLFGIVN
                     RKLVEAGLFADPYEPGRAFKGALPAVCDLDL"
     gene            complement(3588..4373)
                     /locus_tag="PKV64_04800"
     CDS             complement(3588..4373)
                     /locus_tag="PKV64_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPN16434.1"
                     /translation="MKRAHPRKIPPAVAGIILIVLSAPFSVPAAGAEQADLTPEAVLR
                     NVDENAVPGSKIMVSAMTIHGRRGDRTIKSRSWIEGEDKAFTEYLEPPREKGIKMLKL
                     GDQLWTYTPATDRIIGISGNLLRQAVMGSDLSYEDMMENRKWSEIYEAADDGGETILD
                     RPCRILKLTARVADAAYHVRRVWVDRERFLVMREERYGKSGRLLKTLEVGDVRRIGGR
                     WIQTRALVRDVLKEGNGTEFVIESIDFDAPMPEALFSKASLRK"
     gene            complement(4370..5647)
                     /locus_tag="PKV64_04805"
     CDS             complement(4370..5647)
                     /locus_tag="PKV64_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPN16435.1"
                     /translation="MIRFLFKGLLRDRSRTLLPFLTVAIGVLLTVFLECYITGAEHQI
                     IDFNADLLTGQVKVMTKGYAAEPDSLPNDLSLDGVGTLLSELGTDFPNMAWTPRIRFG
                     GLLDVPDEAGETKAQGTVLGTAADILMPGSAEIRRLGLENTVVRGRLPEKRGEILIGE
                     ILADRLKLQPGGTATLIGSTMNGAMATANYTVSGIVRYGVRALDRTGLVMDLADAQAV
                     LDMEDAAGEILGWRTNGPYERKLIEAVAARFNGRTEAGSGGDLAPVMKTLRDQSDLAA
                     LLDQFSTVRGAILAIFILAMSLVLWSAGLLGSLRRYGEIGVRLAMGEPKGGVYRAMLG
                     EAVMVGVLGTVAGTALGLALSYYLQAHGINISGLMKNAAVMLPSHLYTRVTPVAYVIG
                     FIPGLLSTLIGTAIAGRGIYKRSTASLFKELET"
     gene            complement(5644..6816)
                     /locus_tag="PKV64_04810"
     CDS             complement(5644..6816)
                     /locus_tag="PKV64_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPN16436.1"
                     /translation="MIIPKLAFRNLIGNGAKTWLNALVLSFSFVAIIWGQSLFEGMDR
                     EMSKVTIDQEAGGGQYWAGAYDPYDPATIDDAPAAIPADLAAEAAAGRAAPILIVSAT
                     IYPKGRMFPALLKGVDPDQDVLKLPFDRLLGGDGEIPGLIGARTAKSTGLAKGDTVTV
                     RWRDASGTFDAADVVVVDVFQTQVQTVDSGLIWVPLETLQAMTGRPGRATVVTVPVGS
                     APRSYPGWGFQDYDVLLKDIKSFVQAKSLGFSIFYVTLMALALLAIFNTQILSIWHRR
                     REIGMLIALGMPRGRVIALFTLEGALLSILAAAVGAVYGLPLLIYMSKHGWVLPAGQA
                     DSFGLALDKALYPAYSTGLVFGTILLVLAATTIVSFLPTRKIVTLKPTDALKGKWS"
     gene            complement(6827..7525)
                     /locus_tag="PKV64_04815"
     CDS             complement(6827..7525)
                     /locus_tag="PKV64_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN16437.1"
                     /translation="MENSVLIRTVNLTKIYPSGEQPITALRDVNLVFQAGEFAGLVGP
                     SGSGKTTLLNIVGSLDVPTSGSVEVLGEKIETLSHKKAARLRNRHIGFIFQTFNLLPV
                     YTVYENVEFPLLLLGMTAAARRKAVRDALAWVGLSDREHSRPAQLSGGQSQRVAIARA
                     VVKKPDLVLADEPTANLDADNSHHILHTMEDINRDLGTTFLFSTHDAKVIAHLRRKIT
                     LVDGRVTADEARTT"
     gene            complement(7539..8702)
                     /locus_tag="PKV64_04820"
     CDS             complement(7539..8702)
                     /locus_tag="PKV64_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16438.1"
                     /translation="MKRSGKAPLAPVVFFFAGALCLSAGPLLSQETDFRGQVSGELAI
                     PFESGRRVPLRVRYLPQFSLKTPVSASWNFDIEASVNASLTLDAASGWGSRKGDLALY
                     RLWARLTTARFEARLGLQKIEFGSARLLRPLMWFDRLDPNDPLQLTDGVTGLLLKYTF
                     QSNVSVWAWGLTGNNDRKGWESFPTRAGTIECGGRAQVPALGGELAVTFHRRSLDAFR
                     GPLPLASDERSGVPESRLGLDGYWDLGPGLWFEAAFTKQDFQVFPFRHQKAVNLGVEN
                     TFGLGNGLTLLAEHLLFQSSPRFWERGTSRSLSALSLDYPLGLLDRLRVMVFRDWTGR
                     DWYRLLTWQRTYDRLSLNVIVFWNPDSYGLYGDPAAVNLFGGRGVYLMVAWNH"
     gene            complement(8793..9974)
                     /locus_tag="PKV64_04825"
     CDS             complement(8793..9974)
                     /locus_tag="PKV64_04825"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HPN16439.1"
                     /translation="MTVKHIILVGDGMADDPHPALGGKTPLEAAATPNMDRIAARGRV
                     GRVLTVPAGFPPGSDVANLSLLGYDAAAHYQGRGPIEAASIGAALDPDDTAFRCNIVT
                     LGDGLMADYSAGHITTAESRPMIEELQAALGTDRVRFVPGISYRHLLIIRDFPAGLDC
                     TPPHDISGREWASHLPKGPGRELILDLMEKARPVLAANAANRKRATPATDIWPWGQGR
                     AMRLPTLEQRFGLRGSVISAVDLVRGLGVLAGLKVRLVDGATGYLGTNYAGKVAAAKE
                     ALAGEDFVYLHVEAPDETSHEGELAKKIQAIEEFDRHIVGEILGWAESKPGVRILVAP
                     DHATKLATKTHAADPVPYAVCGPGVVRDGGSAYNERAAESVPLEAAVGLFERFIRGTF
                     K"
     gene            complement(9982..11472)
                     /gene="thrC"
                     /locus_tag="PKV64_04830"
     CDS             complement(9982..11472)
                     /gene="thrC"
                     /locus_tag="PKV64_04830"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPN16440.1"
                     /translation="MRYIGTHANHPFASASEAVLLGMVPRGGLFVPEFIPRLEADTLT
                     APDYLETARRIMAAYFTDFEPPVLDGCLRRAYNPDNFHTPDVVALRPLGRRRFLLELF
                     HGPTAAFKDVALQLMPHLTATAKSLTGDQTHTLILVATSGDTGKAALEGYKNADGISI
                     VVFYPDGGVSEVQRLQMVTTDGRNTRVFAVRGNFDDCQTGVKALFLDANLRDYLAGLN
                     IKLSTGNSINWGRLAPQIVYYIRAYALLAAGGEIKMGDEVDICVPTGNFGNILAAWYA
                     RAMGLPVRKLFCASNKNNILTDFFQTGVYDTHREFFRTSSPSMDILISSNLERFLFEI
                     AGRDAEKIKPWFLDRFRTGRFEIDAETKKKIDAFVVSGWADEPRVFAEIKTVHEETGI
                     IIDTHTAVASAVSRDVGPADGPATIIASTASPFKFARDVLGAVTGQAEPDEFRAIDRL
                     SELAGRPAHRAVRGLRERPVLHEGVLPISQMREAVIGFAERVRNRG"
     gene            complement(11699..13186)
                     /locus_tag="PKV64_04835"
     CDS             complement(11699..13186)
                     /locus_tag="PKV64_04835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPN16441.1"
                     /translation="MTRRTSGTTWGIFLFWGAALVLSFLNLSGDLMNDDEGTYLYAAW
                     RTGLGEVPYRDFSLVQAPLGFYLGAAVFKLFGPAVWPARALSVLFLLGAAFLVFESAR
                     RFLKLKTGFALAAAGVFLFNKHVFFLGRSFMPDVPMIFFGTAALFFALRAESSGPDGG
                     RASQAALLAGVMAGLSALAKINGVLIFAGYPAFLFLEAVRRRKTWKTAASRVASAAAG
                     FAASFAVPFGFFLVFVPGTFDAVVGFQAARESAASVPFILQAGRRIAHLIGNHNYGLA
                     AGALAALSAGRAGAEENAAGLRRIRVLLWTSAAAVLAVIFVPGDFFIRYAVFAFVPLA
                     LLFGAGGEALAEGKTIGRRITLGFVAALTLLCLGPTLSPKKILARDEGTRAAAAFIRQ
                     NTKPADYVFGDDPFLNFLARRPCPPDLVDVSEAMIEAGRITTAEVVEDCDLFNVKMVF
                     VETGVSAHHLVKLKDYAAFRGYLSRRFRPAAAIRREFLNVEFFIR"
     gene            13350..14030
                     /locus_tag="PKV64_04840"
     CDS             13350..14030
                     /locus_tag="PKV64_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPN16442.1"
                     /translation="MTKDMARRREEGTKASRRKALTLIFDFDGTIADTLAAIVRLANK
                     YNEDLGIPPLDRTEIEAMRGMSSREILRRHRIPLAKLPYLLLHYQKELGREIDKLGLF
                     PGIREVLLELKALGVRLGILTSNSTENVEKFLQARELEVFDFIHSEKNLFGKTRALRH
                     VMKEEDVGREEVMYVGDEARDIEACRKARVPVIAVSWGFQHREFLADHDPTYIVDSPD
                     EILEIVTA"
     gene            14027..15211
                     /locus_tag="PKV64_04845"
     CDS             14027..15211
                     /locus_tag="PKV64_04845"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HPN16443.1"
                     /translation="MKPAAAGLYVHFPFCRRKCPYCHFYSLPAETAGIRPRVEAWRKG
                     IAAEAALFRSGRSVAGSAVHETPLVFDTLYLGGGTPSLADPGDVARLKDDLAGRLPLQ
                     PLEFTLEANPSGPENEERSDSARFRGWIRAGVTRLSVGAQSFDDGVLRVLGRPASADR
                     TVSFVRRARDAGFDSLSLDLMAGVPGETPRSWDRTLDAVRLLRPDHVSLYFLENVEGL
                     PLEKVLREHPVDEDAAVDAFERAADALAETGLRRYEISSFALPGKECRHNFKYWRYEP
                     FLGLGPSAASHIGAKRWSNAAGFEEWRAGTAAGAVPPAEIVELDPESAAREALAAGLR
                     LVEGIDLESFAGRFGFRPDERLRPAIESLGRDGLIVLSSGILRIPKDKLLISNAILSR
                     LL"
     gene            15466..16695
                     /locus_tag="PKV64_04850"
     CDS             15466..16695
                     /locus_tag="PKV64_04850"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN16444.1"
                     /translation="MDASTGKAYGYRWLVLAAYVLIAGVCEVLWITFAPVTGTAAAFY
                     HTSDLMIGLLSMSFMVVYIPMFYPAAWLIDKKGFRASVGLGAVMTAVFGLARGIFASN
                     FTLVFISQVGLAAAQPFIIGATTTIASRWFPVRERATATGLGTLALYLGPLVAMILTP
                     ALVLRVGFEEMLWIYGLAAALAAFVFLWIAREHPPVPPEPGAPGERILVFEGLKTMFR
                     RRDVLFLMTIFFVGLGLFNAVSTWIEDIVRPRGFTISQAGWLGGLMLLGGIIGAIVMP
                     VLSDKIARRKPFILIALAGLIPGLIGMTFTTSLPVLLAAGFVFGFFLLSAGPIGFQYA
                     AEITRPIPEGASNTVLLLMGQVSGILFILAMDAFKSKATGSMTGSLAALTVMIIGSFG
                     LALFLPESLKKPAASRP"
     gene            complement(16625..17494)
                     /locus_tag="PKV64_04855"
     CDS             complement(16625..17494)
                     /locus_tag="PKV64_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPN16445.1"
                     /translation="MGRLSFADKLVVVTGAASGLGRALARRLAVVEKARLIAADCRAD
                     RLASLKADIESACPSRVYPVPVDLGAPGGAKILFDKAAATGTVYALINNAGLSFYGKT
                     LDRPFAAYRELIQVNCLTVMETTALFLEYFLAKGEGAILNVTSLGGLISLPYQNVYAA
                     TKHAVQSFSEGLSAEYRGQGVVICTFAPGGIATEMISRSGLDRHFRMDSSLLMPPERA
                     ARRALRAFKNKRMVCVPGFLNKLAVFSQRVASRARVLRTTERIYSPKNVRAGRRPASS
                     GTRGEREPGRRNR"
     gene            complement(17625..17963)
                     /locus_tag="PKV64_04860"
     CDS             complement(17625..17963)
                     /locus_tag="PKV64_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HPN16446.1"
                     /translation="MIARIWHGAVKTPRKDAYYEYMMKTGVPWYKSHEGNRGVLVLQK
                     TSPETTDFLLLSFWDSMDAVRKFAGENPELAVYDFDEDKDFLLEMESKVAHYEIRSGF
                     IGSGDGVLCS"
     gene            complement(17960..19057)
                     /locus_tag="PKV64_04865"
     CDS             complement(17960..19057)
                     /locus_tag="PKV64_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HPN16447.1"
                     /translation="MPDPPAAGEPQTTLWELAGHELDDPGAVLARVRRPAGEIGFPAG
                     PGKWPEGNSMNGLLEELACHGFRARTVSADHAPELKAEIEERHRRGEFAEEFYRERLL
                     DIRSSFNDAEPEIRTLIVVAAPQPIIRLIFHLPVKPVTLVVPPTYQYGVNEKAERLLK
                     KCLKRAGCRLIRTSLPLKLLAARSGLGVYGRNNVCYVKGLGSFHRLMAFGTDLPWEED
                     SWGPARLMDTCSRCRACRRLCPTGAIQEEDFLLRAHRCLTFLNERSDPFPQWTDSSWH
                     HAVVGCLHCQLVCPENKTVRNWSQAGDKFSEEETRLLLEGRRPEKIPADLRGRLERLG
                     LLEYSEILPRNIQAALANSASSADSLLEEYA"
     gene            complement(19304..20083)
                     /locus_tag="PKV64_04870"
     CDS             complement(19304..20083)
                     /locus_tag="PKV64_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPN16448.1"
                     /translation="MESDAGLIREAQAGDREAVNELIKRERRYLFNFLHQLTEDPATA
                     DDLTQETFCRALPKLSGFNRRSSFRTWLTKIALNVFRNDYRKRKRHASLCLDRIVVPA
                     GDGNPERLVIKSELQWCVVHTLRHHLPRLYREALVLRDLHSFSYEDIAAIIGGKPGQA
                     KTRVHRARKLFQNRLMKDRCRAFTDDYLCVCEEILELDADRISAGFPDARGECERLQT
                     TRWIIKKLKEVPAPESSAAGTPSAGRPFRSSAPRRNRRKPV"
     gene            complement(20225..20704)
                     /locus_tag="PKV64_04875"
     CDS             complement(20225..20704)
                     /locus_tag="PKV64_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16449.1"
                     /translation="MIIEYFHASKFGNGILVAGEFKERMASRGVEVNIRHIREAKPKK
                     LPPADLYLFSSPGRIGKPRGCARRFLKKIRLTPGTKYALLTTEGAPRPDKNGRMPTEE
                     ETAKWQRVRPIMNKILEGKGLVKVAEDKILVTGMKGPLEEGWRDKVEAFASRIPLPS"
     gene            complement(20875..21816)
                     /locus_tag="PKV64_04880"
     CDS             complement(20875..21816)
                     /locus_tag="PKV64_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16450.1"
                     /translation="MTMKTETRANGRGASPWAAIGVTVLFAALICGILPAERTDQFKG
                     AGIRSSTYGRPDDPGPGYWARTGTEIAAKFPGAKPEAIWIVSRLKGRGTEMSFPVPPG
                     TDPLITGMSEDISEPVLKLFDELGYRVWLQTEPGWAPIEEVFHVMLERYGHHKCVVGV
                     GVDVEWHRSNNPDAGDPVTDEMAEAWLAEARSHDPSYRMFLKHFRQDVMPPAARDGIL
                     FVDDSQIFDSADAMIAEFAEWGKSFAPAPVGFQYGYRTDKPWWGPMKDPVKEIGDRIL
                     AAVPNTEMLIWVDFSIQDVFPPDPAKGDRVKTIVKNP"
     gene            complement(22164..24968)
                     /locus_tag="PKV64_04885"
     CDS             complement(22164..24968)
                     /locus_tag="PKV64_04885"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN16451.1"
                     /translation="MKKCFAVLALAFLAVSFGFGQIGQTGQITVTVVDKDGVPLGGVT
                     VTITSPDMVVARMDRVTNPSGVARFPSLNPGTYEIKCEITGFNTTVRKDIIVNAGITS
                     AIDVDMAEAAIGEEVVVVGLSPTVDRQSTTKTSTLDTNFVANIPASRDLDTLFNMTPG
                     VTAGTAHGSTVRDNAYSVDGVNVTDSTIGMLANDFSLNIAEEVSIQSGGLSAEYGQVR
                     GAVVNVVSKSGGNEFHGKVDLFYRGKNFQSNNTNGTPLEGQISGFDYEIEPGLSLGGP
                     IIRDKLWFFANVSFFQSRSYVAGFPYDTPGKQVPVGDTRPYPYLKLTFQPNQDNKFVI
                     SYGYSDSLNKNGYASMYDTEATTMDISTHTHIINALYTHFFSSNVFLNVRASAWISGL
                     FRFAKTQTPAFWEYATYRNSGSAGWDDDNPRRHVSVEANGTTFLEDFAGSHELKFGVS
                     FRRAIEIRECTMHEVPGTMWSFISTNNGDPYYAYFYDDIRWVNVLRDYGFYVNDTWNP
                     IKNLTVNLGLRFDINQGLWPPQNEDEDYYQLPFEGALKVDRSIKETMTVYNWVNLSPR
                     IGFSYDIFGDGKTFLKGSFAKYTRGNINTWAGRGNPNAWTYFGAYINPDYSLYAVDWV
                     QYAGKELAPQYGYKDYDLKSPYTNEFTLGVERELFPDWSVGVRYIAKKEKNIIDDADS
                     NALDLDKLLANGEMDWIGYEPVTAVDPYNGASVTFYNRLPQADVSRIYLINPPNAKTN
                     YSGVEVTLDKRYSNNWQVHASYTYAKSSGLVATDFSATSAWGTGLYQNPNAHTNAEGR
                     FPYERRHQLKVSATVKIPFGINLGTYTRFLSGQRYTRTISARRIGLLTLRQWDETIYA
                     ETLGSRGLPALFICDLRAEKEFKLGKITIGLFGEVFNLFNVNKATAVFTDSSNPYYPF
                     EQMSAIQDPRIFRIGARFEL"
BASE COUNT         4615 a         7656 c         8156 g         4830 t
ORIGIN      
        1 agcgtcggca ccatgatcga gatggtgatg atgttcaagg tcttcatcag gacgttgagg
       61 ttgttgctga cgatggaggc ccgggcatcc atcaagctgg ccaagatgtt cgagtagacc
      121 tcggcttggc ggtggcactg gctgttctcg atgaccaggt cgtcgagcag ctccatctcc
      181 tcctgggtga atccgaaccg gccggcgttg tgccgcatct tctcgatcag cacgccgttg
      241 gagccgaccg cgttctggta gtaaaccagg ctcttctgca gggcgaagag gttgatgagg
      301 tatttgttct ccatggcctt gttgatcttg tgctcgacgt cgtcggccat catgttgatg
      361 atccgcaggt gctccatgaa atggtaaatg gcccggtaga cgagccggat ggtcagctcg
      421 cggagagagg tgacccgggc cagttgcttc ccttcgaaga tgaccgtgtc gtcggccgcg
      481 accaggatga ggacgtcgcc gaagaagaac gcgcccatgg tcgaggcttt gaacaggagc
      541 tggtcgtccc cggaatagtt cttgggccgc ttgaagatga aggcgatatg gttcggctcg
      601 cattcgaggc gggactgctc gtcggggtcg agggcggaag ctagggtgtg ctcgtcgatc
      661 tgatattcgt ccacaagtcg tcgcttttcg gattcgtcgg ggttgagata gagaaggacg
      721 gcggccttgt cttcggccgt ttcgacgatt ttttggttca cgatctgata gcgcttcagc
      781 atcgtccggc tcctttccgc tccgtctgga tgtttgaggc tcattatgcc ccgccgcccg
      841 gacgaaggca agaccttcgc gcgcttttcg accgcgaatt cgtcaggaga tgtgttcgac
      901 ggtctcctgg taaacgacgc cgcgctcgcg aagggcgtcg agcaggaagg ccgtgcactc
      961 cggccgccgg ccgatgattt cgggaggcac gattcccttg tccgcgaaca ggccgccggc
     1021 caggaggcgg agggcgcacg acgccgtgta gcctgtcgtc cgggccatgg aatgaatccc
     1081 ggtcgccggg tcgcggcggt cgagcaggtc atacgtgtac cggagggcgt ttcccaggcg
     1141 ccggccttcc acgatgattt tcatcacggt caggtccgat tctccttccc gcagcttcca
     1201 gcgggagaag agaagccggg cggtgacgtc ccggggccgg acccggacgc cgccgacctc
     1261 gatctcgtcg gtgtcgaaaa agcccgaatc ccggaacatg gccatttttt cgacatggcc
     1321 gggataacgg agggttttct ccttcatgtg ggggacccgc atcgttcggg cgagcgtccg
     1381 gagaccgtcg gtgttgaaag cctccagcgt cccgacgccc gggaattcca gccgctcgag
     1441 ctcggagagg gccggcttca cgaccagctc gccgttatgg atatagcggg ccgggcgggt
     1501 gtattcctcc aggacgtcgg ccggcgagaa cacggccttg tattcataag gccattcccg
     1561 aatctccggc aggcccccga cgtagatcag aatggattgg gtctcctcga gccgggaagc
     1621 ggcgtaaccg gccaggatgt tcgacatccc gggagcgacg ccgcagtcga cgacggcggt
     1681 gacgccggcg gcttccgcct gttcctggag aagaaacgga tcctcgggga aaaaggcgat
     1741 gtcgacgacg cttttccggg cggcgatcac ggccccaagc gtctgaaaac ccatgaatcc
     1801 gggaaccgcg ctcaggacga ggtcgtaatt ttcgaccagg cgggtcacgg ttttcggatc
     1861 ggcgaggtcg acccggaccg tccggatgtt cggctttccc tcgaggcggg cgagggcttc
     1921 ggcgtcccga tcggccgcgg tgacttcgaa ccgcgcctcc gcggccaggt cgagggccgt
     1981 cggcgctccg acgagtccgg ctcctaaaac gacgattttc atcggcatgc tccttgtgat
     2041 ttcgcgcggc gcttgagagg ggcggaaacc ccggaaaaag cctggaaagg gcggaagggg
     2101 cgggttacaa gccgctgaac cggtcgatat aaatggaaag gtgtttcggc acggtctttt
     2161 cattttatcc gatccggccg gattttcaag agaatccccg gccccgtcaa ctttttcggc
     2221 cgggcggcgt atttaaaaag agaggcctgg aatgaataaa tcgaccgttc atttgatttt
     2281 tctttgggcc gccgttcttg cggcgggctc gcttcttgcg aacgccgcct ccgccggcgg
     2341 ccgggccgcc gatgagcggc tgcttcaata cagcatcgtc ggctcgtcct cctgcgaccg
     2401 gacgccgtgt ttcaacgggg tcatggcgcg gatccccgtc cagcggtttt tgctcgagct
     2461 ggccgaggga cccatggccc gggcgaaagc cgaagcccgg gacctcggcg ccgcggggat
     2521 cgcggatctc ttgaagctcc ggctggtccg gttcgacggc gaccgttatc tcctgaattt
     2581 cgccctcttc acggccgccg atgtcggccg catccgcgaa gtcagcgagc gctatgcggg
     2641 atcgctggcc gaagcgctgc tagcccggcg ggcggacatc gagaccgccc tccgggctta
     2701 cgacgtcccg ggcgtggatc ccggctcggt cgcctatttc ctcctcggct gcgtttccct
     2761 ggattgggac ggcctggccg tgacggatga aaaaaaatac cgcaagacga cggaggaacg
     2821 gttcgacgga agctacgtgc ccgccgccga agagctcggg gaaacgtcgt tggagaaaat
     2881 ctactggggc agccactacg aagagttcag cggagtccat ttcacctcgt tcggcgacca
     2941 tcatgccctg cctcggatgc tgttccccga cctgctttgg aatgttcccg gtctttcctc
     3001 ccgctatccc gaggcggtga ggaatgccca ccgggagctg ttgtcggact cgctccgccg
     3061 cggcggagct aagattggac ggatgatgct ggccctgcgg gagggcccga aagcggccgg
     3121 ggatctcgcc cgggcggcgg ggacggatga agacgaatcc cgcgtctttc tgcgcctgct
     3181 ttccgttctc gaatacgtcc gggaaaagga cgggtggtac gaaacccgga tccccgtcct
     3241 gaccggacgc gacgccgtca tgaccgccga gctgcgggcg atcggccggc gggtgatgga
     3301 acaatggctg gccggaaatt atgaccgcct cgccgccgac ctcgacggcc tcagcttcac
     3361 ccgttccggg gtgccgttca gcgagggctt caccatgatc tggcactatc ttttcggaat
     3421 cgtcaaccgg aagctggtcg aggcgggcct gttcgccgat ccctatgagc cggggcgcgc
     3481 cttcaaggga gccctcccgg cggtctgcga cctcgatctc tgatcagcca gagacgcgtc
     3541 cccttttccg ccggcctgat atgcgggtcg agaagcccgc ggcttcctca ttttctcagg
     3601 gacgccttgg agaagagcgc ctcgggcatc ggggcgtcga aatcgatcga ctcgatgacg
     3661 aactccgtgc cgttcccctc tttgaggacg tcgcggacca gggcccgcgt ctggatccaa
     3721 cggcccccga tccgccgaac gtcgccgacc tcgagcgtct tgagaaggcg cccgctcttg
     3781 ccgtagcgct cctccctcat gacgagaaac cgctcccggt cgacccacac ccgccggacg
     3841 tggtaggccg cgtcggcgac ccgggccgtc agcttgagga tccggcaggg gcggtcgagg
     3901 atcgtctccc cgccgtcgtc ggccgcctcg tagatctcgg accacttcct gttttccatc
     3961 atgtcctcat acgacaagtc cgagcccatg acggcctgcc ggagcaggtt tccggaaatg
     4021 ccgatgatcc ggtccgtggc cggggtgtac gtccagagct ggtcgcccag cttcagcatc
     4081 ttgatcccct tctcgcgcgg aggctccaga tactcggtga aggccttgtc ctcgccctcg
     4141 atccaggagc gggacttgat cgttcgatcg ccccgccggc cgtggatggt catggccgag
     4201 accatgatct tgctcccggg cacggcgttc tcgtcgacgt tccggaggac ggcctccggc
     4261 gtcaggtccg cctgctccgc tcccgcggcc gggaccgaaa acggcgcgga caaaacgatc
     4321 aggatgattc cggcgacggc cggcggaatt ttccggggat gggcgcgctt catgtttcaa
     4381 gctccttgaa aagggacgcg gtcgatcgct tgtagatgcc tctcccggcg atggccgtcc
     4441 cgatcagggt ggagaggagg ccggggatga agccgatgac gtaggcgacg ggcgtgaccc
     4501 gggtgtacag gtgggacggc agcatgaccg cggcgttttt catcagcccg gagatattga
     4561 tcccgtgggc ctgcagatag tagctcaggg ccagcccgag ggcggtcccg gcgaccgtgc
     4621 caaggacgcc gaccatgacg gcctcgccca gcatcgcccg gtacacgccg cccttcggct
     4681 cgcccatcgc cagccggacg ccgatttcgc cgtagcgccg gaggctgccg agcaagccgg
     4741 cgctccagag gaccagcgac atggccagga tgaagatggc caggatcgct ccccgaaccg
     4801 tgctgaactg atcgagcagg gcggccaggt cgctttggtc gcggagcgtt ttcatgaccg
     4861 gggcgagatc gccgccggag ccggcctcgg tccggccgtt gaacctggcg gcgacggcct
     4921 cgatgagctt ccgttcgtag gggccgtttg tccgccagcc caggatttcc ccggccgcgt
     4981 cctccatgtc gaggaccgcc tgggcgtcgg ccaggtccat caccagcccc gtccggtcca
     5041 gggcccggac gccgtagcgg acgattccgc tgaccgtgta attggccgtg gccatggctc
     5101 cgttcatcgt cgagccgatc aacgtcgccg ttcccccggg ctggagcttg aggcggtcgg
     5161 ccaggatttc gccgatcaaa atctcgcctc gcttctccgg cagacggccc cggacgaccg
     5221 tgttctccaa gcccaggcgc cggatctcgg cgctccccgg catgaggatg tcggcggccg
     5281 ttcccaggac cgttccctgg gctttcgtct ctccggcttc atccgggacg tccagaaggc
     5341 cgccgaaacg gatccgaggg gtccaagcca tattgggaaa gtccgttccc aattcgctga
     5401 gaagcgttcc gacgccgtcc agcgagaggt cgttggggag ggaatccggt tcggccgcgt
     5461 agcccttcgt catgaccttc acctggcccg tcagcagatc ggcgttgaaa tcgatgattt
     5521 ggtgttccgc gccggtgatg taacactcga gaaagacggt gagcaaaacc ccgatcgcca
     5581 cggtcaggaa gggaagcaaa gtccgtgaac ggtcccggag aagccctttg aacagaaagc
     5641 ggatcatgac catttcccct tcagggcgtc ggtcggttta agggtgacga ttttccgcgt
     5701 cggaaggaag ctgacgatgg tggtcgcggc cagcacgagg agaatcgtgc cgaagaccag
     5761 cccggtgctg taggcgggat acaacgcttt gtcgagagcc aagccgaagc tgtcggcctg
     5821 cccggccggc aggacccagc cgtgtttgga catgtaaatc aggaggggca ggccgtagac
     5881 cgccccgacc gcggccgcca ggatcgaaag gagcgccccc tccagggtga agagggcgat
     5941 gacccggccg cgcggcatgc ccagggcgat cagcatcccg atctcccggc ggcggtgcca
     6001 gatggacaag atctgggtgt tgaaaatcgc cagcagggcc agggccatca gggtgacgta
     6061 aaaaatcgaa aagccgagcg atttcgcttg aacgaagctc ttgatatcct tgaggagaac
     6121 gtcgtaatcc tggaagcccc agccgggata ggatcgaggc gcggacccga cggggacggt
     6181 gacgaccgtc gcccggcccg gccggccggt catggcctgg agggtctcga gcggcaccca
     6241 gatcagcccg gaatcgacgg tctggacttg ggtttggaag acatcgacga cgacgacgtc
     6301 ggccgcgtcg aacgtcccgg aggcgtcccg ccagcggacg gtcacggtgt cccccttggc
     6361 caagcccgtg cttttcgccg tccgggcgcc gatcaagccg gggatttcgc cgtcgccgcc
     6421 gagaaggcgg tcgaagggca gcttgagaac gtcttggtcc gggtcgaccc ccttgagcag
     6481 ggcggggaac atccggccct tgggatagat cgtcgcggag acgattagga tgggggcggc
     6541 ccggccggcc gccgcttcgg cggccaaatc ggccgggatc gccgccggcg cgtcgtcgat
     6601 cgtcgccggg tcgtacgggt cataggcgcc ggcccaatac tggccgccgc cggcctcctg
     6661 gtcgatggtg accttggaca tttcccggtc cattccctcg aacaggcttt ggccccagat
     6721 gatggccacg aaggaaaacg agaggaccag ggcgttgagc caggttttcg ccccgttgcc
     6781 gatgaggttg cggaaggcga gcttggggat gatcatggcc gcctcctcac gttgtccggg
     6841 cctcgtcggc ggtcacccgg ccgtcgacga gggtgatttt ccggcgcagg tgggcgatga
     6901 ccttcgcgtc gtgcgtcgaa aacaggaacg tcgtcccgag atcgcggttg atgtcctcca
     6961 tggtgtggag gatgtgatgg gaattatccg cgtccaggtt ggccgtgggc tcgtcggcca
     7021 ggacgaggtc gggcttcttg acgacggccc gggcgatggc gacccgctga ctctgcccgc
     7081 cggataattg ggccggccgc gaatgctccc ggtcggacag gccgacccag gccagggcgt
     7141 cccgcacggc cttgcgccgg gccgccgccg tcatcccgag aagaagcagg ggaaactcga
     7201 cgttttcata gaccgtgtag accggcagca agttgaatgt ttggaagatg aagccgatgt
     7261 ggcggttgcg gagccgggcg gccttcttat gcgagagcgt ctcgattttt tcccccagga
     7321 cctcgacgct tcccgatgtc gggacgtcga gcgacccgac gatgttgagg agggtggtct
     7381 tgcccgagcc gctcggcccg acgagcccgg cgaattcgcc ggcctgaaag acgagattga
     7441 cgtcccggag ggcggtgatc ggctgttccc ccgaagggta gatcttggtc aagttcaccg
     7501 tccggatcaa aacgctgttc tccatgacat cctctccttc agtgattcca ggcgaccatg
     7561 agatagacgc cgcggccgcc gaagagattc acggcggccg gatctccgta aagcccgtac
     7621 gaatcgggat tccagaaaac gatgacgttc aagctgagcc ggtcgtatgt tctttgccag
     7681 gtcaggagac ggtaccagtc ccggcctgtc cagtcgcgga aaaccatgac ccggaggcgg
     7741 tcgagaagac ccagagggta atcgagggaa agggcgctca gggagcggct cgttccccgc
     7801 tcccagaacc ggggggatga ttgaaagagg agatgctcgg ccaggagggt gaggccgttc
     7861 cccaggccga acgtgttctc caccccgagg ttgacggctt tttgatggcg gaaggggaag
     7921 acctggaaat cctgcttggt gaaggccgcc tcgaaccaga ggccggggcc gagatcccaa
     7981 tagccgtcca gccccaaccg gctttccggg acgccggaac gctcgtccga agcaagagga
     8041 agaggcccgc ggaaggcgtc caggctccgg cggtgaaagg tcacggccag ctccccgccg
     8101 agggcgggaa cctgggcccg gccgccgcac tcgatcgtgc cggcccgggt cggaaaggat
     8161 tcccagccct tgcggtcatt gttgcccgtc aatccccagg cccagacgga aacgttgctt
     8221 tgaaacgtgt acttcaggag gaggccggtc accccgtcgg tcagttggag cggatcgttg
     8281 gggtcgagcc ggtcgaacca catgagagga cggaggaggc gggccgagcc gaactcgatc
     8341 ttctggaggc cgagccgggc ctcgaaccgg gccgtcgtga ggcgggccca aagccggtag
     8401 agggccaggt cgccttttcg cgagccccag cccgaggcgg cgtcgagcgt gagcgaggcg
     8461 ttgaccgacg cctcgatgtc gaaattccaa gacgccgaga cgggcgtttt gagggaaaac
     8521 tgcggaaggt agcggacgcg gagagggacg cggcggccgc tttcgaaggg aatggccagc
     8581 tcgccggaga cctggccgcg gaaatccgtt tcctgggata aaagcggccc ggcggaaaga
     8641 cagagggcgc cagcgaaaaa aaagacgacc ggcgcaagcg gagctttccc cgaacgcttc
     8701 atagagggaa ggatgggttg gaaagggacg tcttgtcaag ggctgaatga atgaaacgga
     8761 ttgagcgcgt ccgccctcgg gagccgcgtc ccctatttaa aagttccgcg gatgaagcgc
     8821 tcaaacagcc cgacggccgc ctcgaggggg acgctttccg cggcccgttc attgtaggcg
     8881 gatccgccgt cccggacgac gcccgggccg cagacggcgt aaggaaccgg gtcggcggcg
     8941 tgggtcttcg tcgccagctt ggtcgcgtga tccggagcga cgaggatccg gacgccgggc
     9001 ttgctctccg cccagcccag gatttcgccg acgatgtgcc ggtcgaattc ctcgatggcc
     9061 tgtattttct tcgcgagctc gccctcgtgg ctggtttcgt cgggcgcctc gacatggaga
     9121 tagacgaaat cctcgccggc caaggcttcc ttggcggccg cgaccttgcc cgcgtaattc
     9181 gtcccgagat agccggtcgc cccatcgacg agccggacct tgaggcccgc cagcacgccc
     9241 agtccgcgga cgagatccac ggccgagatg accgatcccc tcaatccgaa gcgttgctcc
     9301 agcgtcggca gacgcatcgc ccggccttgg ccccagggcc agatgtcggt ggccggcgtc
     9361 gcccgcttcc ggttggccgc gttggccgcc aggacgggac gggctttctc catcaggtcg
     9421 aggatcagct cccggcccgg ccccttcggc aggtgggagg cccattcccg gccggaaatg
     9481 tcgtgcggcg gcgtgcagtc caggcccgcc ggaaaatcgc ggatgatcag caggtgccgg
     9541 tagctgattc ccgggacgaa acggacgcgg tccgttccca gggcggcctg gagctcctcg
     9601 atcatggggc gcgattccgc ggtcgtgatg tggccggccg aataatcggc catgagcccg
     9661 tcgcccaggg tgacgatgtt gcaccggaaa gccgtatcgt ccgggtccag ggcggccccg
     9721 atgctggcgg cttcgatcgg cccccgtccc tggtagtggg ccgcggcgtc gtaccccagg
     9781 agcgataggt tggccacgtc gcttccgggc gggaagccgg ctggaacggt gaggacgcgg
     9841 ccgacgcggc cgcgggcggc gatccggtcc atgttcggcg tggccgccgc ttccaagggc
     9901 gtttttcctc cgagggcggg atggggatcg tcggccattc cgtcgccgac gaggatgatg
     9961 tgcttcaccg tcattccccc ctcagccccg attccggacg cgttcggcga agccgatgac
    10021 ggcttcgcgc atctgggaaa tcggaagcac gccctcgtgg aggacgggcc gctcccgcag
    10081 cccccggacg gcccgatggg ccggccggcc ggccagctcg ctcaggcggt cgatcgcccg
    10141 gaactcgtcc ggctcggctt gcccggtgac ggcgcccagg acgtcccggg cgaacttgaa
    10201 cgggctggcg gtcgaggcga tgatggtcgc cggaccgtcc gcggggccga catcgcggga
    10261 gacggccgag gcgacggcgg tgtgagtgtc gatgatgatc ccggtctctt cgtggaccgt
    10321 cttgatttcg gcgaagaccc ggggctcgtc ggcccagccg gagacgacga acgcgtcgat
    10381 ttttttcttt gtttcggcat cgatctcgaa ccggcccgtc cggaagcggt cgaggaacca
    10441 gggcttgatc ttctccgcgt cccggccggc gatttcgaac agaaaccgtt cgagattgga
    10501 ggagatcaag atgtccatcg agggcgacga cgtccggaaa aattcgcggt gagtgtcgta
    10561 gactccggtt tggaaaaagt ccgtcaggat gttgtttttg ttggaagcgc agaagagctt
    10621 gcggacggga aggcccatgg cccgggcata ccaggcggcc aggatgttgc cgaagttgcc
    10681 ggtcgggacg caaatgtcga cctcgtcgcc cattttgatt tcgccgccgg cggcgagcag
    10741 ggcgtaagcc cggatgtagt agacgatctg gggggccagc cggccccagt tgatggaatt
    10801 gcccgtcgag agcttgatat tcagcccggc caggtagtcg cgcaagttgg cgtcgaggaa
    10861 aagggccttg actccggttt ggcagtcgtc gaagttgccc cggacggcga agacccgggt
    10921 gttccggccg tcggtggtga ccatctggag gcgctgaacc tcgctcacgc cgccgtccgg
    10981 ataaaacacg acgatggaaa tgccgtcggc gttcttgtag ccctccaggg cggccttgcc
    11041 ggtgtcgccg gacgtggcga ccaggatcag agtgtgggtt tgatccccgg tcagggattt
    11101 ggccgtggcg gtcaagtggg gcatcagctg gagggcgaca tccttgaaag cggccgtcgg
    11161 cccgtggaac agctcgagga ggaagcggcg ccggccgagc ggccggaggg cgacgacgtc
    11221 gggcgtgtgg aagttgtccg ggttgtacgc ccgccggagg cagccgtcga ggacaggcgg
    11281 ctcgaaatcc gtaaagtaag cggccatgat ccgccgggcc gtctcaagat agtcgggggc
    11341 ggtcagcgtg tccgcttcca gccgggggat gaactccgga acgaacagcc cgccccgggg
    11401 gaccatcccc aggaggacgg cctcggacgc ggaggcgaag ggatgattgg cgtgagtgcc
    11461 gatgtaacgc atggtcgcct cgagacgcgg tagaattaga cgtaaggatg ttatcattca
    11521 ggggcgcctc actcaacccc ttgcggaggt ccacgtcagg ggaaacgctt ttgctttgga
    11581 ggcgcatttc cagcgccggc gatcggatgt ccccggaccc ggggatcaag gagccggagg
    11641 ccgttgaagc ttggaaggac atggggaggg agggcggcct ttaaggaggc ttcaccgatc
    11701 agcggatgaa gaactcgacg ttgaggaatt cgcgccggat cgcggccgcc gggcggaatc
    11761 ggcggctcag gtagccgcga aacgcggcgt aatccttcag cttgaccagg tgatgggcgg
    11821 agacccccgt ttccacgaag accatcttga cgttgaaaag gtcgcagtcc tcgacgactt
    11881 cggcggtcgt gatccggccg gcctcgatca tggcttccga gacgtcgacc aagtccggcg
    11941 ggcagggccg ccgggcgagg aagttgagaa acgggtcgtc gccgaagaca taatccgccg
    12001 gttttgtgtt ttgccggatg aaggcggccg ccgcccgcgt cccctcgtcc cgggccagga
    12061 tctttttcgg gctcagggtc gggccgaggc agagcagggt cagggcggcc acgaaaccga
    12121 gggtgatcct tcggccgatg gttttcccct cagccagggc ttcgcctccc gcgccgaaga
    12181 gcaaggccag ggggacgaaa gcgaaaacgg cgtatcggat gaaaaaatcg ccggggacga
    12241 agatgacggc caggaccgcc gcggcggatg tccagagaag gacgcggatc cggcggaggc
    12301 ccgcggcgtt ctcctccgct ccggctcgtc cggccgaaag cgcggccagg gcgccggccg
    12361 cgagaccgta gttgtggttt ccgatcaagt gggcgatacg gcggccggct tgaaggatga
    12421 aagggacgga ggcggcggac tccctggcgg cttgaaaccc gaccacggcg tcgaacgttc
    12481 cgggaacgaa gacgagaaaa aaaccgaacg gaacggcgaa cgaagccgcg aatcccgcgg
    12541 cggccgaggc gacccgggag gccgctgttt tccaggtttt ccgccgccgg acggcctcga
    12601 gaaaaagaaa cgccggatat ccggcgaaaa tcagaacgcc gttgatcttg gccagggcgg
    12661 acaagccggc catcactccg gccagcaggg ccgcctggga cgcgcgaccg ccatccgggc
    12721 cggagctttc cgcccgcaaa gcgaaaaaaa gcgcggccgt gccgaaaaag atcatcggga
    12781 cgtcgggcat gaaggagcgg ccgagaaaaa aaacatgctt gttgaagaga aaaacgccgg
    12841 cggcggcaag ggcgaagccg gtcttcaatt taagaaatcg gcgggcggac tcgaaaacaa
    12901 ggaaggcggc gccgagcagg aagagcacgg agagagcccg ggcgggccag acggcgggtc
    12961 cgaagagctt aaaaaccgcg gcgcccagat aaaagccgag cggcgcctgg acaagggaga
    13021 aatcgcgata ggggacttcg ccgagcccgg tccgccaggc ggcgtaaagg taggtcccct
    13081 cgtcgtcgtt catcagatcc ccggaaagat taagaaagct caagaccagg gcggcgcccc
    13141 agaaaaggaa aatcccccag gttgttccgc tcgtccgccg cgtcatgatt tctcccttat
    13201 agtggatggc gcggactcaa gtcaaattcg aggggaaaaa cccgccgtcg gcgcgtcttc
    13261 tcgttctcgc cgcgcgattc gtcagagaga cgatcgaagg ttgacaaaaa gaaaatcggc
    13321 tcttatgatt gaaatctaaa gagatcccga tgaccaagga catggccaga cgccgggaag
    13381 aagggacgaa ggcgtcccgg cggaaagctc taaccctcat tttcgatttc gacggcacca
    13441 tcgccgacac gctggcggcc attgtccgcc tggccaacaa gtacaatgaa gacctgggga
    13501 ttcccccgct cgatcgaacg gagatcgagg ccatgcgcgg catgtcgagc cgggaaatcc
    13561 tcaggcggca ccggatcccg ctggccaagc tgccgtatct ccttctgcac tatcaaaagg
    13621 agctcggaag ggaaatcgac aagttggggc ttttccccgg catccgggag gttctcctgg
    13681 agcttaaagc gctcggcgtg cggttgggca tcctgacgtc caattcgact gaaaacgtcg
    13741 aaaagttcct ccaggcccgc gagctggagg tgttcgattt catccactcc gagaaaaacc
    13801 tgttcgggaa aacgcgcgcc ctccggcacg tcatgaagga ggaggacgtc gggagggaag
    13861 aagtcatgta cgtcggcgat gaggcccggg atatcgaggc ctgccggaaa gcccgcgttc
    13921 cggtcatcgc cgtcagctgg ggcttccagc atcgggaatt cctggccgac catgacccca
    13981 cttacattgt cgattccccg gacgaaattc tcgaaatcgt cacggcgtga agcccgcggc
    14041 cgccgggctc tatgtccatt tccccttctg ccgccggaag tgtccgtact gccattttta
    14101 cagcctcccg gcggagacgg ccggaatccg gccccgggtg gaagcctggc ggaaaggaat
    14161 cgccgcggaa gcggccttgt tccgctccgg ccggtccgtc gcgggctcgg cggttcacga
    14221 gacgccgctc gtcttcgaca cgctctatct gggcggggga acgccgtcct tggccgatcc
    14281 cggcgacgtc gcccgtctca aggacgacct ggccgggcgg cttcccttgc agccgcttga
    14341 attcaccctg gaagccaatc cctccggtcc ggaaaacgag gagaggagcg attcggcccg
    14401 gttccgcgga tggatccggg ccggcgtcac ccgcttaagc gtcggcgccc agtccttcga
    14461 cgacggcgtc ctccgcgttc tcggccgccc ggcgtccgcc gaccggaccg tttcgttcgt
    14521 ccggcgggcc cgggacgccg gctttgattc cctcagcctg gacttgatgg ccggcgtgcc
    14581 gggcgaaacg ccgcgttcct gggaccggac tctggacgcc gtccggcttc tccggcccga
    14641 ccatgtctcc ctctatttcc ttgaaaacgt cgaaggcctg ccgttggaga aggtcctgcg
    14701 ggagcatccc gtggatgaag acgcggccgt cgacgccttt gagcgcgcgg ccgatgcgtt
    14761 ggcggagacg ggcctgcgcc ggtacgagat ttccagtttc gcccttcccg gaaaagaatg
    14821 ccgccacaac ttcaagtact ggcgctatga gccgtttctc gggctggggc cgtcggccgc
    14881 ctcccatatc ggagccaagc gctggagcaa cgcggccggc ttcgaggaat ggcgggcggg
    14941 gacggcggcc ggggccgtcc ccccggccga aatcgtcgag ctggatccgg aatcggccgc
    15001 ccgcgaagcc ctggccgccg gcctccgcct cgtcgaggga atcgatctcg agtcgttcgc
    15061 cgggagattc ggctttcgtc cggacgagcg gctccggccg gcgatcgaaa gcctcgggcg
    15121 ggacgggctg atcgtccttt cgagcgggat cctccgcatt ccgaaggaca agcttctcat
    15181 ctccaacgca atcctttccc ggcttctctg accgcggccg cgttcccctt gaggagactc
    15241 acgctcagct cttatttata taatcagacc attccgcgaa tctccccttc gatccatcac
    15301 ggcaggggat cacgaacccg ggcatcctgg acatattgcg gcctgcccct ccattcagac
    15361 tcagcccgct ttcaatgttt acgtgacatt tataatgacg gggttgagag atccgcccct
    15421 aaatgttaga gtttcccatc atctgcaaga aaagaggaga aactcgtgga cgcatcgacg
    15481 gggaaagcat acgggtaccg ctggctggtt ttggccgcat atgtcttgat cgccggcgta
    15541 tgcgaggtgc tctggatcac cttcgccccg gtcaccggga cggcggccgc cttctatcac
    15601 acctccgacc tgatgatcgg gcttctctcc atgagcttca tggtggttta cattcccatg
    15661 ttctatccgg ccgcctggtt gatcgacaag aagggcttcc gggcgtcggt cggcctgggg
    15721 gcggtcatga cggcggtttt cgggctcgcc cggggcattt tcgcttcgaa tttcaccctg
    15781 gttttcatct cccaggtcgg cctggccgcc gcccagcctt tcatcatcgg ggcgacgacg
    15841 acgatcgctt cccggtggtt ccccgtccgg gagcgggcga cggccacggg tctcgggacg
    15901 ctggccctct acctcggtcc gctggtcgcg atgatcctga ctcccgccct cgtcctccgc
    15961 gtcggcttcg aagaaatgct ctggatttac ggcttggccg cggccctggc cgccttcgtc
    16021 ttcctctgga tcgcccgcga acatccgccc gttccgcccg aaccgggcgc ccccggggag
    16081 cggatcctgg tgttcgaagg cttgaagacc atgttccggc gccgggacgt tctgtttttg
    16141 atgaccatct tttttgtcgg cctcggcctg ttcaacgccg tctcgacctg gatcgaagat
    16201 atcgtccggc cccgcggatt cacgatttcc caggccggct ggctgggcgg cctcatgctc
    16261 ttgggcggga tcatcggggc gatcgtcatg cccgtccttt ccgacaagat cgcccgccgg
    16321 aagccattca tcctcatcgc cctggccggg ttgattccgg ggctcatcgg aatgaccttc
    16381 accacgagcc tcccggtcct cctggccgcc gggttcgtct tcggattctt tctcctgagc
    16441 gcgggaccga tcggttttca gtacgcggcc gagatcaccc gcccgattcc ggaaggagcg
    16501 tccaacacgg ttctccttct catgggacag gtttcgggca ttcttttcat cctggccatg
    16561 gacgcgttca aatcgaaggc gacgggatcg atgaccggat cgctggcggc cttgaccgtc
    16621 atgatcatcg gttccttcgg cctggctctc tttctccccg agtccctgaa gaagccggcc
    16681 gcctcccggc cctgacgttt ttgggggagt aaatccgctc ggtggtccgg agaacgcggg
    16741 cccggctcgc gacccgctgg gaaaagacag ccagcttgtt caagaatccg ggaacgcaga
    16801 ccattctttt attcttgaac gcccggagcg cccggcgggc ggcccgctcc ggcggcatca
    16861 ggagggagct gtccatccgg aagtgccggt ccaggccgga cctggaaatc atctcggtcg
    16921 cgattccgcc gggggcgaac gtacagatca cgacgccttg tccccggtat tccgcgctca
    16981 gcccctcgct gaacgattgg acggcatgct tggtcgcggc gtagacgttc tggtagggaa
    17041 gggagatgag cccgcccagg ctcgtgacgt tcaagatcgc cccttccccc ttcgccagga
    17101 aatattcgag aaaaagagcc gtggtctcca tgaccgtgag acagttgact tggatgagct
    17161 cgcgataggc ggcgaagggc cggtcgagcg tctttccata gaagctgagg ccggcgttgt
    17221 tgatcagggc gtagacggtt ccggtggccg ccgccttgtc gaaaaggatt ttcgccccgc
    17281 cgggcgcgcc gagatcgacc gggacgggat aaacccggga ggggcaggcg gattcgatgt
    17341 ccgctttgag ggacgccagc cggtcggccc ggcagtcggc cgcgatcagg cgggcttttt
    17401 caacgaccgc cagccgccgg gccagggcgc ggccgagccc cgaggcggcc ccggtgacga
    17461 cgacgagctt atcggcgaac gataaccggc ccattcctct tcatcgtacg ccagcggccg
    17521 gagagattca atccggtttg aaccaagggc ggaaaagcgg atgtttggtc cgaaccccga
    17581 aaagggggag gatttggccg gcgatcagct cagcgttccc ttgctcatga acaaagaact
    17641 ccgtcgccgc tcccgataaa tcccgagcgg atttcgtaat gggccacctt ggattccatc
    17701 tcgagaagaa aatccttgtc ctcgtcgaag tcgtaaacgg ccagctccgg gttttcgccg
    17761 gcgaattttc ggacggcgtc catcgaatcc caaaaggaca ggagaaggaa atccgtcgtt
    17821 tcgggggacg tcttctggag gacgagcacg ccccggtttc cctcatggga tttgtaccag
    17881 ggaacgccgg ttttcatcat gtattcgtaa taagcgtctt ttcgaggtgt tttgacggcg
    17941 ccgtgccaga ttcttgcgat catgcgtatt cctccagaag ggaatccgcg ctcgaggcgg
    18001 agttggccag ggcggcctgg atgttgcgcg gcaggatttc gctgtattcc agaagaccaa
    18061 gccgttcgag ccggccgcgc aggtccgcgg ggatcttctc cggccgcctt ccttcgagaa
    18121 gaaggcgcgt ttcttcctcg gaaaacttat cccccgcctg ggaccagttt cggaccgtct
    18181 tgttctccgg gcagaccagc tggcaatgca ggcagccgac gacggcgtga tgccaggagg
    18241 aatccgtcca ctgcggaaac ggatcggatc tttcgttcaa aaacgtcaag cagcgatggg
    18301 cgcggagcag gaaatcctct tcctggatcg cgcccgtcgg gcagaggcgc cggcaggccc
    18361 gacatcggga gcacgtgtcc atcagccggg ccggccccca ggaatcttct tcccagggaa
    18421 gatccgtccc gaatgccatc agccggtgaa agctgcccag gcctttcacg taacaaacgt
    18481 tgttccggcc ataaaccccc aggccgctcc gggcggccaa cagcttcaag ggaagagatg
    18541 tccggatcag ccggcagccg gcgcgtttca ggcatttttt cagaagccgt tccgccttct
    18601 cgttgacgcc gtactggtag gttggaggca cgaccagggt gacgggtttg acgggcaggt
    18661 ggaagatgag gcggataatc ggttggggcg cggcgacgac gatcagggtg cggatttcgg
    18721 gctcggcgtc attaaaggaa gaccggatat ccaggagacg ctcccgataa aactcctcgg
    18781 cgaattcgcc ccgccggtgc cgctcctcga tttcggcttt cagctcaggc gcatgatcgg
    18841 ccgagacggt ccgggcgcgg aatccatgac aggccaattc ctcgagaaga ccattcatcg
    18901 aattgccctc cggccatttt cccggcccgg cggggaagcc gatttccccg gccggccgtc
    18961 ggacccgagc gagaacggcc ccgggatcgt cgagctcgtg tccggcgagt tcccaaagcg
    19021 tcgtttgggg ctcgccggcg gccggcgggt ccggcatccc atgctgacca tcacggttcc
    19081 ctggacgatc atcgccgcgg ccatgatccg gcaggcgatg attccgaaaa gttcgcctct
    19141 ttgggccggg aaacgaggcg tttcgctgtc gagttcaaaa cccttcatga ataatcctcc
    19201 ctcgggcgcg gcgatttccc aaatgcccga attcggccta agccggattc gagaacgtta
    19261 gacaatcgaa cgagggccgg agttacaaaa ccggaaatgg gtatcagacg ggtttccgcc
    19321 ggtttcttcg tggagccgat gatcgaaacg gccgccccgc cgacggcgtc ccggccgcgg
    19381 agctttcggg cgcgggaact tccttcaatt ttttgatgat ccaacgggtc gtttgaagcc
    19441 gctcgcattc cccgcgggca tccgggaaac cggccgaaat ccgatcggcg tcaagctcga
    19501 ggatctcctc gcagacgcac aagtaatcgt ccgtgaacgc ccggcaccgg tccttcatca
    19561 accggttttg aaaaagcttt ctcgcccgat gaacccgcgt tttcgcctgc cccggcttgc
    19621 cgccgatgat cgcggcgatg tcctcgtagc tgaaggaatg cagatcccgc agaaccaggg
    19681 cttcgcgata gaggcgggga agatggtggc ggagggtgtg gacgacgcac cactggagtt
    19741 cgcttttgat gaccagccgt tccgggttgc cgtcgccggc gggaacgacg atccggtcga
    19801 gacaaagcga agcgtgccgc ttccgcttcc ggtagtcatt ccgaaagacg ttcaaggcga
    19861 ttttcgtcag ccatgttcgg aaactcgacc ggcggttgaa accggacagc ttgggcagag
    19921 cccggcagaa ggtttcctgc gtcaaatcgt cggccgtggc cggatcctcc gtcaattgat
    19981 gcaggaaatt aaaaagatac cgccgctccc ttttaataag ctcgtttacg gcttcccgat
    20041 cgcccgcttg ggcctcccgg atcaggccgg cgtcgctttc catccgccgg gaggaagacg
    20101 tgcctttcgt ctctgcggat ccgggggaaa gcggtcttcg gcggccgtca ttcaccgttt
    20161 ttcttccgga atccgatgat cggcgggcgg atcatttccg ctcccatcga tgatgacggg
    20221 aagatcagga cgggagcgga atccgcgagg cgaacgcctc cactttgtcc cgccagcctt
    20281 cctccagcgg ccctttcatg ccggtcacca ggatcttatc ctcggcgact ttgaccagcc
    20341 ccttcccctc gaggatttta ttcatgatcg ggcggacgcg ctgccacttg gcggtctcct
    20401 cctcggtggg cattcgcccg tttttgtccg gccggggcgc gccctcggtg gtgagaaggg
    20461 cgtacttcgt tcccggcgtc agccggatct ttttcagaaa acggcgggcg catcctcggg
    20521 gcttgccgat gcgtcccggc gagctgaaca gatacaggtc ggccggcggc agttttttcg
    20581 gtttcgcttc ccgaatgtga cggatgttga cctcgactcc ccgggaggcc atccgctcct
    20641 tgaattcccc cgcgaccaag attccgttgc cgaacttcga agcgtgaaaa tactcgatga
    20701 tcatggctcc tccccctttt tgatcaggcg tctccggcgg ccttcttggc gttcctcgtc
    20761 ttggtcaggc aggcgacgag gatgttcatg gggaaatctc cttcccggct accctttaaa
    20821 tgccctgaaa aacagtaatg atgaggatat ctttattcaa aaaaacaaga tctgtcaagg
    20881 atttttaacg atcgttttaa cgcggtctcc cttggccgga tccggcggaa agacgtcctg
    20941 gatcgaaaaa tccacccaga tcagcatctc cgtgttcggc acggcggcca ggatccggtc
    21001 gccgatctcc ttgacggggt ccttcatcgg gccccaccac ggcttgtcgg tccggtagcc
    21061 gtattggaag ccgaccggcg ccggggcgaa cgatttgccc cactcggcga attcggcgat
    21121 catcgcgtcg gcggaatcga agatctggct gtcgtcgacg aacaggatgc cgtcgcgcgc
    21181 ggccgggggc atgacgtcct ggcggaaatg cttgaggaac atgcggtagg aaggatcgtg
    21241 ggaccgggcc tccgccagcc aggcctcggc catctcatcg gtcaccggat cgccggcgtc
    21301 gggattgttg gagcggtgcc attcgacgtc gacgccgacg ccgacgacgc acttgtgatg
    21361 gccgtagcgc tcgagcatca cgtgaaagac ctcttcgatg ggcgcccagc cgggctcggt
    21421 ctggagccag acgcggtagc ccagctcgtc gaagagcttg aggacgggct cgctgatgtc
    21481 ctcggacatc ccggtgatga gggggtcggt tcccggcgga accgggaacg acatctcggt
    21541 gccgcggccc ttcagccggc tgacgatcca gatggcctcc ggtttcgccc cggggaactt
    21601 ggcggcgatc tcggttccgg tgcgggccca gtaccccgga ccgggatcgt ccggccggcc
    21661 gtaggtggag ctccggatgc ccgccccctt gaactgatcc gttctttccg ccggcaagat
    21721 tccgcaaatc aaggcggcaa agaggaccgt caccccgatc gccgcccacg gcgatgcgcc
    21781 gcggccgttc gctcttgttt cggttttcat cgtcatggtc cttttcctcc cggaatcgat
    21841 tcgctgactt cgaagatatc accggaaaga ggcggattca agcgcctgaa ctagttcagc
    21901 gcctcgttcc caaatgcttt aatgcttgta tgaaggccct tagaattcgc gatggcaatt
    21961 caggctccgt tttttcgaag cgccaggggt tcagccgggt caacgcatct tcttaaagac
    22021 cgcgtcctgg acgggaggcc cgccgggggg ccgggaataa ggcggggacg cttcccggga
    22081 gaagcatccg gggatataaa aaaggggcgg ttcccgaagg aaccgcccct ttccgcgaga
    22141 ccggcgagtt cgctcttctc agctcacagc tcgaaccggg cgccgattct aaagatccgc
    22201 gggtcttgaa tcgccgacat ctgctcaaac gggtaatagg gattgctgga gtccgtgaaa
    22261 accgccgtcg ctttgttgac gttgaacaaa ttgaagactt ccccgaacag gccgatggtg
    22321 attttcccga gcttgaattc tttctcggct ctcaaatcgc agatgaacag agccggcaag
    22381 ccccgggaac cgagcgtctc ggcgtaaatc gtctcgtccc actggcgaag ggttaacagg
    22441 ccgattctcc gggcgctgat ggtccgagta taccgttggc ccgacaaaaa cctcgtgtac
    22501 gtgccgagat tgattccgaa ggggattttc accgtcgccg agaccttcaa ctggtgccgt
    22561 ctctcataag ggaaccgtcc ctcggcgtta gtatgggcgt tcggattctg atacaatccc
    22621 gtcccccagg ccgaggtggc gctgaaatcg gtggccacca ggcccgacga cttggcgtac
    22681 gtgtaggaag cgtgcacctg ccaattattg gaatatctct tgtcgagcgt cacttccacg
    22741 ccggagtaat tcgttttagc gttgggcgga ttgatgagat aaattcgaga cacgtcggct
    22801 tggggcagcc tgttgtagaa cgtcacggag gcgccgttgt acggatccac cgcggtgacg
    22861 ggctcatagc cgatccagtc catttctccg ttggccagga gcttgtcgag gtccagcgcg
    22921 ttggagtccg cgtcgtcgat gatgttcttt tccttcttgg cgatgtacct gacaccgacg
    22981 gaccagtccg ggaacagctc ccgttcgacg ccgagggtga attcattcgt gtacggggac
    23041 ttcagatcat agtccttgta tccgtattga ggggcgagct ccttccccgc gtattggacc
    23101 cagtcgacgg cgtacaacga ataatccgga ttgatataag ccccgaaata cgtccaggcg
    23161 ttgggatttc cgcggccggc ccacgtgttg atattgcccc gggtgtattt cgcaaagctc
    23221 cctttcagga acgtcttgcc gtcgccgaaa atgtcatagg aaaagccgat ccggggggaa
    23281 aggttcaccc agttatagac cgtcatcgtc tctttgatgc tccggtcgac tttaagggcc
    23341 ccctcgaaag gcaactggta atagtcctcg tcctcgttct gcggcggcca caggccttgg
    23401 ttgatgtcga atctcaagcc gagattcacc gtcaggttct ttatcggatt ccaggtgtcg
    23461 ttgacgtaga atccatagtc gcgcaggacg ttcacccacc ggatgtcatc atagaaatag
    23521 gcatagtagg ggtctccgtt gttggtactg atgaaagacc acatcgtccc gggcacctcg
    23581 tgcatcgtgc attcacggat ttcgatggcc cttctaaacg aaaccccgaa tttcaattcg
    23641 tgggatccgg cgaaatcctc gaggaacgtc gtgccgttgg cttccacgga aacgtgcctt
    23701 ctcggattgt cgtcatccca tccggcgctt ccggaattcc ggtatgtcgc atattcccag
    23761 aaggcgggag tctgcgtttt ggcaaaccgg aacaatccgg atatccaggc cgaagctctc
    23821 acgttcagga agacgttgga gctgaagaaa tgggtgtaaa gagcgttgat gatgtgggta
    23881 tgcgtcgaga tatccatcgt cgtcgcctcc gtgtcataca tggaggcata tccgttcttg
    23941 ttgaggctgt cggaataccc gtaggaaatg acgaacttgt tgtcttgatt cggctggaag
    24001 gtcagcttga gataaggata cggcctcgta tcgccgacgg ggacttgttt gccgggagta
    24061 tcgtaaggga aaccggccac gtaagaacgg ctttggaaaa aactgacgtt ggcgaaaaac
    24121 caaagcttgt ccctgatgat cgggccgccg aggctgaggc ccggctcgat ttcataatcg
    24181 aagccgctga tttgtccctc gaggggcgtt ccattcgtgt tgttggattg gaaatttttc
    24241 ccccggtaaa acagatccac tttgccgtgg aattcgtttc cgccggactt gctgacgacg
    24301 ttgacgacgg ctcctctgac ctggccgtat tcggccgaca atccgccgct ttgaatcgag
    24361 acttcttcgg caatgttcag gctgaagtca ttggccaaca tgccgatcgt cgagtccgtg
    24421 acgttgacgc cgtcgacgga ataggcgttg tcccggaccg tggagccgtg ggccgtgccc
    24481 gccgtcactc caggcgtcat gttgaacaac gtgtcgaggt ccctggacgc cggaatgttc
    24541 gccacgaaat tcgtgtcgag agtcgacgtc ttggtcgtgc tttgcctgtc gacagtcggg
    24601 gagaggccga cgacgacgac ttcctcgccg atcgcggctt cggccatatc gacatcgatg
    24661 gccgacgtaa tgcccgcatt cacaatgatg tctttcctga ccgtcgtgtt gaatcccgtg
    24721 atttcgcatt taatttcgta tgtcccggga ttcaatgagg gaaacctggc gacgccgctg
    24781 gggttggtca cccggtccat tctcgccacg accatgtccg gagacgtgat ggtcaccgta
    24841 acgccgccaa gcggaacgcc gtctttatcg acgaccgtga cggtgatttg tcccgtttgg
    24901 ccgatctggc cgaatccgaa cgaaacggca agaaaggcca gggcaaggac agcgaaacac
    24961 tttttcatct caacctcctt gaaaaatatg gcattagaga tttttgtgtt gcctttagaa
    25021 aaccggtagc ctttcgtggc tgtttttaac ggtcttgtca ttaagaaact caatgtcgag
    25081 tttttccgag ggagaaattc ctccaaaatt tagggccaaa agaacgacct ctttcggccg
    25141 aacaggccgg ccttgactcc atttggggcc cctcttcaga caatacaaac aagtttttcc
    25201 tgcttcgata tatcaggtag atatattaca cgcaatatat agcatatatt gtgccaa
//
LOCUS       DAOOXH010000027        25045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_1791_length_25045_cov_8.646819, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000027 DAOOXH010000000
VERSION     DAOOXH010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 25045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25045
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1791_length_25045_cov_8.646819"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PKV64_04890"
     CDS             <1..873
                     /locus_tag="PKV64_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16452.1"
                     /translation="KAVPRPKATQPAPVEPPPSVYEATREIMAAAAAESPVSLFLWGG
                     GGITAVGDLNDGIQGLADFYADQIHRPAGGETAPLRTGLQYGGEIGFKLLPRLRLSVG
                     AEFLRASKKNVLAFAGDSEIPSDLTVKPSFSAIPVHLTLAYYPLDFLYLKAGAGYFFT
                     RAEYRYVFTHGDILRDWEGEATASGAGFLAGIGLDLSLGRYFCLTAEASGRYAKISGF
                     SGTNAYRDEISTEPYLETGTLYAFDVRTTSRTAYPLVFIRNGKPFESGVENAREARLD
                     LTGLSVRIGLKYRF"
     gene            complement(870..1526)
                     /locus_tag="PKV64_04895"
     CDS             complement(870..1526)
                     /locus_tag="PKV64_04895"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN16453.1"
                     /translation="MREYEKRRYRGLDQRIVHARETKIIRKMLAEAAKDAAGRDGMRR
                     VLDMPCGYGRFTAMLRDRGLEVVGADLSLEMARRAAENTAGGAPEEGGAGRGIPVLAA
                     DARRLPFRAGAFDLVFSIRLFHHIHSPESREAILGEFSRVSSAYALVSFYRANGLHEV
                     QRRLRRAFGKSRTNIKMVEPGVFEKAAGEAGFSVVRVLPLFGGIHAYHLALLKKRPAD
                     "
     gene            complement(1543..2865)
                     /locus_tag="PKV64_04900"
     CDS             complement(1543..2865)
                     /locus_tag="PKV64_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16454.1"
                     /translation="MRFKFEWNDMADQPHNVAPRSDRTRHHLRRWPSYLELIGLNLVW
                     DPAIFWRYRALRRRLFRRPVDITPGMFGLAVSPRFGRPGETDAVLALLRETGVRRVLF
                     RFPSWERDRLEDYAGFADRLHSEGFEPAAALLQRREDVFAVEAWTDFAESAFDRLGPA
                     CPYFEIGHAWNRTKWGVWDHREYIRLARAAAAARDRIGRKNIKLIGPAVIDFEYHLYP
                     PVLRAVDFDVISSLLYVDRVGAPENPQYGFTAAKKIAFFRACADVLVPGGRPAWITEF
                     NWPLENTGAWSPAAGKPNVGEDVQADFLVRYYVIALASGWIDRAYWWQLVAPGYGLVD
                     SRESPWRKRPSYAAFAFMASMLSGSVFTGKEETPRGGGTAGRGGEVYHFRKGGREFAL
                     AWTRGGPLRREFDRPVAGVLDRSGRGLAQDRDGGLIRLDGSPRYVIFK"
     gene            complement(2873..3703)
                     /locus_tag="PKV64_04905"
     CDS             complement(2873..3703)
                     /locus_tag="PKV64_04905"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HPN16455.1"
                     /translation="MEIRPLAVPPYRGFRTASREGEAAAAALEDFPGFLRSPEAETLQ
                     AGRNQVIAVRLSPDRDAVVKIFGTRGVQKWKTLLLPSKGLRAWKGALALVENGFETAE
                     PIAVFERRRGGIAVESVFISGRVRGGREIREHFLQSSEAVLRRLLSSLAPVLARLHGR
                     GIVHRDLSDGNILVFENEEAAAAPAGGGFRFLFLDTNRVRRRRPGIFNRARNLVRLGV
                     PPGLQPFFLDQYAAAAGGRFRRRRFGLSYRISKRVFISWLAFKKRLRLRALARRLRIQ
                     "
     gene            complement(3703..4854)
                     /locus_tag="PKV64_04910"
     CDS             complement(3703..4854)
                     /locus_tag="PKV64_04910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN16456.1"
                     /translation="MRFEFFGGFLPGYPRGAVLRKGLGRIGAEVVECRFGAGLKSWAR
                     HPLLLLDAFRRGLLRPEGAAAGRCLFVPEFCAKDVPPARLLASLTARPLIFDPLAARY
                     ETKVIDWGWGRPDSPGAWWNFQIDRASFALADLVLADTTAHKRYYCETYGLSPAKVEV
                     VPLGYDDDVFRPPAETELLSRALRPGNGFQVLFFGSFLPLHGVEIIVEAARLVWLRDR
                     SIRFRLIGEGRTSAATRAAAADLANIDFSGRVPMTELARAAAAADVCLGIFGRTAKTR
                     RVVPHKIFQAMGLARPVVTLRTPAVEEFFAHDKNIVLCDKPDPEALARAVLELARDPG
                     RRERIARAGFSLVRREFTPEAVARRLAGFAADHFGPAFALSPPAPGGGR"
     gene            5051..5239
                     /locus_tag="PKV64_04915"
     CDS             5051..5239
                     /locus_tag="PKV64_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HPN16457.1"
                     /translation="MALDSFLLDILACPECQGDVKITADEQGLKCLRCHRVYPIRDDI
                     PVMLIEEAAIEPGRAVKE"
     gene            5241..6305
                     /locus_tag="PKV64_04920"
     CDS             5241..6305
                     /locus_tag="PKV64_04920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPN16458.1"
                     /translation="MNPRSLLLLRFRRLGDIVLTTPAVSLLKSRFPGSSLTYLVEEPY
                     RRLVEGHPDIDRVIVVPAKQNRSDFLRLIREIRRERYDALLDFHGGPRAAWIALLGGA
                     RLKIGHAIKSKGFLYHRTTPRRGLDSPLHSVETHAGLVRALGADFRKEDIPPLILPPA
                     RPEEIARVEALTAAVASGQAGPGGGPLPLVVLHIGAGNAFRDWGEENAAVLASRLLEI
                     PGISLAAVGGEGDRPREDRIAARLGSPRFLSLSGRLNMIEVRELVRRAVLFIGPDSGP
                     MHLAAGTATPIIAYFGPTLPALFGPWRPGLNPAKTVILETNPDCRPCRQRVCEPGDYR
                     CLRNISPDEVLAAVRPFLPS"
     gene            6416..7675
                     /locus_tag="PKV64_04925"
     CDS             6416..7675
                     /locus_tag="PKV64_04925"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPN16459.1"
                     /translation="MMKIIRDFVSGRPAGDIVLASALAAALLFSMISISLAQGFLAIA
                     LIVWLILLARGKRRFETPVFFWALAAYAGLSLLSSAASVNPRISFWDSRDLLLILAVP
                     VTLAAVRRRADLTLALSALFLSAAVNGVYAVFCRFATTAERIRAFMGHYMTQAGLTAL
                     FISLVLGLLFAGFGRRKSAAAGRAEVKGIRIERAVLAAGFLLASAANILTYTRGAWIG
                     LAAALAVAVFLWKPKALILLPVIAALGFLAAPKPVKDRALSIFTMRDDSNVARVQYFK
                     AGVKIISDYPLLGTGPDTVDMVFQDEKYGLGYLAKHNVHLHSNIIQIAAERGLVTLAA
                     WLAFIGLALAGLGRLWRLPETGFSFWAARRKLAAGGLAALTSLFVAGFFEYNFGDSEI
                     AQLLFILITLPFAARRLERESHESAPS"
     gene            7656..8870
                     /locus_tag="PKV64_04930"
     CDS             7656..8870
                     /locus_tag="PKV64_04930"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HPN16460.1"
                     /translation="MNPRRLEKKRRWGRLLRIVLGTAFAVYLAWLALEILSYRKYEAP
                     PKPARTERTMVPPGGPAEASPLSAAPPEPVELRGAYHLHSRHSDGSKTPAELAAAAER
                     AGLDFIILTDHGVPNLASLEAQGRAGRVLVIAGTEISSSRGHLVALGFDPPARPFSQE
                     AGAAAREVHAQGGFTVIAHPYSKTRWSWGSAEDADGLEIINLDGDLRRGWGTALFYAP
                     VLLVKPTLALLKILDPPTAAAGKWDRILAETEERLDPPAAGYYSLDTHRVLHETAFGV
                     LNLHIQLDSLIPADDAAASRAVLDSLRRGRFYNAVDAAADPAGFRFWAEGGPTAPATL
                     HVRAPFPFAHEIRLLRRGEPVAAGTGPELVFNAREPGAYRVEVHLRARTPLPRGVPWI
                     CSNPIIVRKEVP"
     gene            8867..9844
                     /gene="rfaE1"
                     /locus_tag="PKV64_04935"
     CDS             8867..9844
                     /gene="rfaE1"
                     /locus_tag="PKV64_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HPN16461.1"
                     /translation="MTTISLSLPKARRAVLGFRNRRVLVLGDLMLDKYIWGSVSRISP
                     EAPVPVVEVQRSTTALGGAGNVAHNLEALKAAPLLVGVVGDDEEGQWIKKHVPDGRGI
                     FLDPERPTTVKTRIIAQHQQVVRVDQEKKQAIPVGMEDRIVRLIKKEKYAGIIISDYC
                     KGIVTRQMMTRVLAHATEARIPVFVDPKVENFSLFSPVTLITPNHHEAERVVRHSCRT
                     DEEVEQAGREIMEMISCLYLILKRSEQGMSIFERGKKPVHIPTIAREVFDVTGAGDTV
                     IATAALAMLGGLSIREAAVMANAAAGVVVGKIGTATCAPEELLAAVEDH"
     gene            complement(9873..10916)
                     /locus_tag="PKV64_04940"
     CDS             complement(9873..10916)
                     /locus_tag="PKV64_04940"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPN16462.1"
                     /translation="MTSAASPRPRKTLRVLITGATGFVGRHLITRLEAEEKAGRRPRL
                     QITGTCYPERSEECPDFRAAAPRVTLLPLDLRDATAVSAAVAACRPDRIFHLAAISQV
                     RASWNQRRETIETNLMGTFHLLEAARLRAPEARLLIISSSDVYGNQPSRRMPLRETFP
                     LNAASPYGFTKLSAEALAALYARAEKTAVVIARPFTHTGPGQSPDFVCSDWARQIAVI
                     EAAATGKKSGRGPSRVLKVGNTSVRRDYSDVRDVVRAYTLLIEKGRPGEAYNICSGKA
                     RPLREILKMLLRDSREKIVVEVDPARVRRSDIPLLSGSHAKITRETGWRPRIPLETTL
                     SDLLNDWRAKMIR"
     gene            complement(10943..11905)
                     /locus_tag="PKV64_04945"
     CDS             complement(10943..11905)
                     /locus_tag="PKV64_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPN16463.1"
                     /translation="MNILITGITGFAGSHLAEYILAHHPGVKVHGLVRWRSRMENIAP
                     LLHTGKITLYEADLKDMSSLRKALAAARPDRIFHLAAQSFVPASWKLPAETFMINAVG
                     QINLFEAVMAEKLDPRIQIAGSSEEYGLVHPDEVPMKETNPLRPLSPYAVSKVAQDLL
                     GFQYWQSYRLRVVRTRGFNHTGPRRGEVFVTSNFARQIVMIEKGLVPPVIHVGNLEAK
                     RDFTDVRDTVRGYWLALEKGASGEVYNIGTGNAVSMKDLLDRLLALSPAKVEVRIDPD
                     RLRPSDVQILCADVSKFKAATGWSPKITFETTLRDLLEYWRAAL"
     gene            complement(11902..13374)
                     /gene="purF"
                     /locus_tag="PKV64_04950"
     CDS             complement(11902..13374)
                     /gene="purF"
                     /locus_tag="PKV64_04950"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPN16464.1"
                     /translation="MCGVIGIWANGQVFRDLYQGLLAIQHRGQDAAGIITYDGRFHTK
                     KGNGLVRDIFKAAHAERLTGAIGIGHTRYPTIGGGRGEDAQPFQLNAPFGLMMAHNGN
                     VVNYVALKKHLAERHHRLLNSDNDVESILNVFAVALEAQNPDSLTPEIVFRAVESVFR
                     QVIGGYSVVAYIAGQGMAAFRDPFGIKSLSWGQRRDGVVPSYAIASESVSLNIMNFSD
                     IRDIPPGSAIFFDHNREIHMKRIWDKPHSPCLFEWVYFARPDSIIDGVNVYRARVHLG
                     ELLAEEIRKAGLPIDIVVPIPDSARDAAIEIARKLNLKYREALVKNRYIGRTFIMPAV
                     NDRQNMVRFKLNPIAAEFKDRNVLLVDDSIVRGNTARAIVGLVRDCGARNVYFASCSP
                     PLRYPCVYGVDMQTRKEFVARDRNEEEVALRIGADRVIYQTQENLVKAIRMENPNLGR
                     FCAACFDGNYITGDVTPEMLQSIEDQRRKASSGQMELNIT"
     gene            complement(13412..14530)
                     /gene="rfbB"
                     /locus_tag="PKV64_04955"
     CDS             complement(13412..14530)
                     /gene="rfbB"
                     /locus_tag="PKV64_04955"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPN16465.1"
                     /translation="MKQTKRRPVKKAVPSAGRRASGLKAAPSVLERALNGRTILVTGG
                     AGFIGSNFIRMMLAEYPGLRVINLDKLTYAGNLENLADVSCDPRHEFIHGDIRDAALV
                     AGCFERVQGVVHFAAETHVDRSIVDAGEFVLTDVFGTYILLEALRKAPGVEFFVHIST
                     DEVYGSREKGFFRETDSLNPSSPYAASKVGADRMVQAYHTTYGAPTIILRPSNNYGPY
                     QHPEKFIPLFTTHALEGRTLPLYGKGRNVRDWLHVEDNCRAIECLIRRGRLGEVYNVG
                     ANDERTNIEVARRIVKLTGASKNLIKFVPDRLGHDTRYAVNINKIKALRWKRKVDFEE
                     GLEATVRWYMDNRAWWTRIKEKNAAFKSFYESYYKDRK"
     gene            complement(14623..15078)
                     /locus_tag="PKV64_04960"
     CDS             complement(14623..15078)
                     /locus_tag="PKV64_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HPN16466.1"
                     /translation="MIQGVSVKKLRVIPDERGRLMEIFRADDECFEKFGQAYMTTTYP
                     GVIKAWHKHERQADNMACVHGMVKVALCDAREGSPTKGEINQFYMGVHNPLLIHIPAG
                     VYHGWMCTSPEEAIVVNVPTEPYNYKEPDEQRLDPHSGEIPYEWERKDG"
     gene            complement(15075..15959)
                     /gene="rfbA"
                     /locus_tag="PKV64_04965"
     CDS             complement(15075..15959)
                     /gene="rfbA"
                     /locus_tag="PKV64_04965"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HPN16467.1"
                     /translation="MRGIILAGGKGTRLHPLTHSVSKQLLPVYDKPMIYYPLSLLMLA
                     GIRDILVISAPESLEAFRALLGDGRRWGLRFEFREQSEPRGLADAFLVGRDFIGGKPS
                     CLILGDNIFFGHGLPGQLRKCARLKTGAIVFAYPVKDPERYGVIELDSRGRAVSIEEK
                     PAKPRSHFAVPGLYFYDGRASNFAAGLKPSARGEIEITDLNLVYLQRGELRVEEMGRG
                     TAWLDAGTHESLLQAANFVQAVEDRQGLMISCPEEVAFRLGYIDVKRLRRLAEAIPNA
                     YGEYLLRLSREPMGGKKP"
     gene            16068..18200
                     /locus_tag="PKV64_04970"
     CDS             16068..18200
                     /locus_tag="PKV64_04970"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPN16468.1"
                     /translation="MDNPSHRRLLRGLAVILYSLLLALFYWKYVPLVAAYQAVLIPLL
                     LFVAVAAATDVRKGLLGFLFLFPLINNLPYFFKLYDPLPMAPSDLVLFLFFFLGRLIH
                     GVFFPFPPEESGKGPKEPLFTPLRLFAGLVLFSALITFWRYTNFFPLWRPRIYELVTN
                     VKGVSAGGAIMSVVFYSLTYLTGIVFFTLCVRVLRTEGFIKKAVAAFGVGSFLSIGFG
                     FIQKFHSPALGNLPASLRHGLLNATFKDALSFGAFLSMLIPFLAGLLPAVKGRTRLTA
                     AVMTAAAGYFLFHAGSKSALIAAAAALLFLGILMLPAILERRGPLARLVIPLGLAAAV
                     FAGASIYLLISGNFPSENGQRTATIERMEDFASALEIRTAGHWRIAGQMIRDYPLSGV
                     GVGAYIIEMANTAKTLKISIGVPESAENLPLHVGSELGIPGMLLFLWILWEMGRRIFL
                     SFRAAPSGTFPRALIAASAAGLFAFLVNSQSHSYIGSYEIQYLFWFLGSLAFAGDVPS
                     PAAAAGRKPSSRRLGRLFGTILLFLGTAGLLWNSAHSLSLNARTERYRLRQGFGLGPY
                     EKDADGRRFRWSGRYAGLTGRAGAPPLKAWLLASHPDIRVRPVRLRLFAAADLATKPV
                     LLQDLVLKENLWKRIELQAPPGFPESSLFLFEVDRTWNPRKAGVSDDPRDLGLAIGII
                     QAAGPIKPKEKTEAAKPANSPPEKKENS"
     gene            complement(18250..20181)
                     /locus_tag="PKV64_04975"
     CDS             complement(18250..20181)
                     /locus_tag="PKV64_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HPN16469.1"
                     /translation="MGKSETARFESKIAPVFRPTVLKRKVFFLTADAVLIALSVYLSF
                     WLRFDGRIPATFTKGLWVRICVVVVVTITMIALHGLYNVAWRFFGLKDMLKLLQAVTL
                     SYLLLIVIIYAFNWLAGMREIPRSVLLLNYNFTLVLLGMLRISKRALREYHFRKRGLS
                     RGRKKVLIVGAGTAGEQIAREMTGNRKSAHFPIGFVDDDEAKRGTEIHGVKVLGKRQD
                     IPQILHGNGVDEVLIAIPTVNSKDIRKIVELVRSTGEVRSIKLLPGIHDIVEGKVSLM
                     DIKDVEVEDLLGRAPVQINFEGIRAFLKNKRVLVSGAGGSIGGELARTISQFEPGELI
                     LLDNDETELFYVVNRLKNAVAGVLPVVATIRDEAKIDRIFEQTRPEIVLHAAALKHVP
                     ILEHYPEEAVKTNIRGTRILGEAAMRYGAERFVNISTDKAINPTSVMGASKRAGEEIL
                     RALNAMGGTRFISVRFGNVLGSRGSVIPLFKEQIRKGGPVTVTHADMKRYFMAVSEAV
                     LLVLEAATAGKGGEAFVLDMGELVSIDELAREMIRLSGLEPDVDIMIVYTGLRAGEKL
                     YEELIMAEEGAMPTEHRKIFLARNSKPLNAAHIWKSVDRLIDACESGSCRKSEIIPLL
                     REIVPTYVPGPDTSSINHW"
     gene            complement(20241..21437)
                     /locus_tag="PKV64_04980"
     CDS             complement(20241..21437)
                     /locus_tag="PKV64_04980"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPN16470.1"
                     /translation="MAEMKIPYSRVLCDGNELAYVREVLESGWLTTASKTLEFEKKFG
                     AAVGARHACAVNSGTAALHLALEAIGVGPGSKVFVPAMTFTASAEVIRYLNADPVFLD
                     VEYGTSLLTTEILEEAVRRFPEVKTVVVVHYGGQAAAMTVEKGAGILDVCRRKGIRVV
                     EDAAHAFPSRRGGRLVGTFGDVTCFSFYANKTITTAEGGMLVTADDRVAARAKVMRLH
                     GIDRDIWNRYVSDKPSWEYDVVAPGFKYNLPDVSAAIGLAQLERAEALRKGRERCARY
                     YMEALAGIPGLDLPKLQVGFEDHAWHLFPVVLTPKAPLSRDRCVERLADRGIGTSVHY
                     KPLYRMTYYRERYGLNPADYPNSERIWNGCLSLPIYPDLRLEELAYICRSLKEILSAR
                     GGEEAV"
     gene            complement(21456..22049)
                     /locus_tag="PKV64_04985"
     CDS             complement(21456..22049)
                     /locus_tag="PKV64_04985"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPN16471.1"
                     /translation="MKRAFDLGASGLGLLLLSPVFLLVAVLIFLADGRPVLFRQTRIG
                     RAGRPFSLFKFRTMIPASEGEGGGFEPGDSRRVTSFGRFLRKTKIDEWPQLFNVLKGD
                     MSMVGPRPEVSKWVAAYPRRWAVVHRVKPGITDPASIIYRNEEELLAAAADPEAVYRN
                     EILPRKLDLYERYAEGPPRFGRDLGLIFKTIAAVLKF"
     gene            complement(22033..22710)
                     /locus_tag="PKV64_04990"
     CDS             complement(22033..22710)
                     /locus_tag="PKV64_04990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN16472.1"
                     /translation="MTHSDDPVIRLAGRGDLEDMTALHCDSFKFGEHVPAMLGRHYVR
                     AMYRWLVTSAVSYALVAEREGKIVGIVAVCDGPFTRPMFLACLPAFAVSLVRRPALLF
                     NHKLWSRLFRRPDVTSAGRKIADQPGFAQMTIGVVDKNCRGRGIFPALVEETKTYSRR
                     RGSRAIRAGIYKINQPSRRVFIKGRWIETPELETEDTVFYVAYLDEAFAGELGLRSAA
                     ASDEKSV"
     gene            complement(22707..23954)
                     /locus_tag="PKV64_04995"
     CDS             complement(22707..23954)
                     /locus_tag="PKV64_04995"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPN16473.1"
                     /translation="MRVLLLTPLFQPEPNHLKGLAFARELVRRGHDVEVLTGFPHYPA
                     GKVYPGYRIRPWMREILDGVPVTRVMMYPSHDRSAVRRTAGYLSLAGTAALLGPPLVA
                     RPDVVHVYQGPATLAWPAMVFRMMFGTPYVLDVQDMWPESVTVTGMLSLPGVSAVLGA
                     WSKATYRLARKIIVLSNGYKKCLEARGVPEKKIEVVYNWCDERSLPGETDGEGSDPFG
                     LAGRFNVVYSGNFGALQALGGVLDAAFLIEEKRPDIRFVFVGDGVEESALKKKAAGRG
                     QKNVLFIPRQAPESLGRITARADVLLVHLKDDPLARMGIPQKTQASLAAGKPVLLAIR
                     GSAAELAARAGGAWTCRPEDPAALAEAVLGLASLSWNEREMMGRKNAEFYRKELAFSI
                     GVGRMTAVFEEAAVRPRRKEGGR"
     gene            complement(23954..>25045)
                     /locus_tag="PKV64_05000"
     CDS             complement(23954..>25045)
                     /locus_tag="PKV64_05000"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl glucosamine 2-epimerase"
                     /protein_id="HPN16474.1"
                     /translation="EIIRLSRVIPALDRECEHVLVHTGQNYDYELNQVFFEDLEIRKP
                     NRFLEAAGATPCETIGRIIERMDAVLDRDRPDALLVLGDTNSCLAAYPAKRRKIPIFH
                     MEAGNRCFDQRVPEEINRRIVDHLADVNLPYSAISREYLLREGLPPDRVIKTGSPMFE
                     VLHHYLPKIKKSGVLERLKLRPGEYYVVSAHREENVDSSKPFDGLMKILNGLAGDGKK
                     KVVVTTHPRTRKIIEARKPALAPGIMLEKPFGFTDYVKLELEACAVLSDSGTITEESS
                     ILNFPALNIREAHERPEGMEEGAVMMTGLDYENVRRGLAVLATQPRGECRGPRIVADY
                     DVPNVSEKVLRIVLSYTDYVNRTVWRAAD"
BASE COUNT         4438 a         8194 c         7712 g         4701 t
ORIGIN      
        1 aaggccgtcc cccggccgaa agcgacccaa cccgccccgg tcgagccccc gccgtccgtc
       61 tacgaggcga cccgggaaat catggccgcg gcagccgccg aaagtcccgt ttcactcttc
      121 ctttggggcg gcggcggaat aacggccgtc ggggatttga acgacggcat ccaaggcctg
      181 gcggatttct atgccgacca gatccatcgg ccggctggcg gagagaccgc ccccctccgg
      241 accgggctgc aatacggcgg cgaaatcgga ttcaagcttc tcccgcggct gcgtctctcg
      301 gtgggcgccg aatttctccg ggcatccaag aaaaacgtcc tggcctttgc cggcgattcc
      361 gaaatcccgt ccgatctgac ggtcaagccc tcgttttccg caattcccgt acatcttacg
      421 ctcgcttatt atcccctgga cttcctctat ctcaaggccg gagccggcta tttcttcacc
      481 cgggcggaat accgttatgt cttcactcat ggagacatcc tgcgggattg ggaaggggag
      541 gcgaccgcgt cgggagcggg cttcctggcg ggaatcggcc tcgatttatc cttaggccgc
      601 tatttctgtc tcacggccga agcgagcggg cggtacgcga aaatttccgg attctcggga
      661 accaacgcct accgcgacga gatttcaacc gaaccgtatc ttgagacggg caccctctac
      721 gcttttgacg ttcgaaccac gagccggacc gcgtatcccc tggtcttcat ccggaacggg
      781 aaaccgttcg aaagcggcgt ggagaacgcc cgggaagcgc ggctcgatct gaccgggctc
      841 tccgtccgga tcgggttgaa ataccgattc taatccgccg gccgcttttt caacaaggcc
      901 agatggtagg cgtggattcc gccgaacagc ggaaggacgc ggacgacgga gaatcccgcc
      961 tcgccggcgg ctttttcaaa caccccgggc tcgaccatct tgatgttcgt ccggctcttg
     1021 ccgaaggccc ggcggagacg ccgctgaacc tcgtgcaggc cgttggcccg gtagaacgaa
     1081 acgagagcat acgccgagga gacccgggaa aactctccca gaatcgcctc ccggctctcg
     1141 ggggagtgga tatggtggaa caagcggatg gaaaaaacga gatcgaaggc tccggcccgg
     1201 aacggcagcc ggcgcgcgtc ggccgcgagc accggaatcc cgcgtccggc ccctccctcc
     1261 tccggcgccc ccccggccgt gttttccgcc gcccggcggg ccatctcgag cgagaggtcc
     1321 gcgccgacga cctcaagccc ccggtcccgg agcatcgccg tgaaccggcc gtaaccgcag
     1381 ggcatgtcga gaacccgcct cattccgtcg cggccggcgg cgtctttcgc ggcctcggca
     1441 agcatcttcc ggatgatctt cgtctcccgg gcatggacga tcctctggtc gagtcctcga
     1501 tagcggcgct tttcatactc ccgcacgcca cccccggtga atctacttga agatgacata
     1561 acgcggactc ccatcgagac ggataagacc gccgtcgcgg tcctgggcga ggccgcggcc
     1621 gctccggtcc aagacgccgg cgaccggccg gtcgaactcc cggcgaagcg gccctccccg
     1681 cgtccaggcc agggcgaact cccggccgcc cttgcggaaa tgatagactt caccgccccg
     1741 gccggctgtc cctccgcccc ggggcgtctc ctccttgccg gtgaacacgc ttccggaaag
     1801 catggacgcc atgaaggcga acgcggcgta gctcggccgt ttccgccagg gcgactcccg
     1861 gctgtcgacc aggccgtatc cgggcgcgac gagctgccac caataggcgc ggtcgatcca
     1921 tccgctcgcc aaggcgatga cgtaataccg caccaggaaa tccgcctgga cgtcctctcc
     1981 gacgttgggc ttgccggccg ccggcgacca ggccccggtg ttctccaagg gccaattgaa
     2041 ttccgtgatc caagcgggcc ggccgcccgg gacgagcacg tcggcgcacg cccgaaagaa
     2101 agcgattttt ttcgccgccg tgaatccata ctggggattc tccggagcgc cgacgcgatc
     2161 gacgtacaaa agcgagctga tcacgtcgaa atccacggcc cttagaacgg gcggatagag
     2221 gtgatattcg aaatcgatga ccgccgggcc gatgagcttg atgttttttc ggccgatccg
     2281 gtcgcgggcg gccgcggccg cccgggccag gcggatatat tcccggtggt cccaaacccc
     2341 ccatttcgtc cggttccaag catgcccgat ttcaaaatag ggacaggccg ggccgaggcg
     2401 gtcgaaagcg gactccgcga aatccgtcca ggcttccacg gcgaacacgt cctctcgccg
     2461 ctgcagcagg gcggccgccg gttcgaagcc ctctgaatga aggcggtcgg cgaatccagc
     2521 ataatcctcg agccgatccc tttcccacga gggaaaacgg aacagcaccc ggcggacgcc
     2581 cgtttcccga agcagggcca ggacggcgtc cgtttcgccg ggacggccga agcgcggcga
     2641 aaccgccagg ccgaacatgc cgggagtgat gtccaccggc cggcggaaca ggcgtcggcg
     2701 gagcgcccgg tatcgccaaa aaatcgccgg gtcccaaacg aggttgaggc cgatcagctc
     2761 caagtaggac ggccagcggc gaagatgatg ccgggtccga tcggaccgcg gagcgacatt
     2821 gtgcggctga tcggccatgt cattccactc gaatttgaac ctcatgatcg aatcactgga
     2881 tccggagccg gcgggcgagg gcccggaggc ggagcctttt tttgaaagcc agccaggaga
     2941 taaaaacccg cttggaaatc cggtaagaca aaccgaaccg tcgccgccgg aaccggccgc
     3001 ccgccgccgc ggcgtattgg tcgagaaaaa acggctggag tccgggcggc acgcccaggc
     3061 ggacgagatt ccgggcccgg ttgaaaatcc ccggccgccg gcgccggacc cggttcgtat
     3121 cgagaaacag aaaccggaat ccgcctccgg ccggagcggc cgcggcctct tcgttttcaa
     3181 aaacgagaat gttgccgtcg gaaagatcgc gatggacgat cccccggccg tgcaggcggg
     3241 cgagaaccgg ggccaacgag gaaagcaggc ggcgcaaaac ggcctcggaa gactggagga
     3301 agtgctcccg gatttcccgt ccgcctctga cgcggccgga gatgaagacg ctttcaacgg
     3361 cgattccgcc gcggcggcgt tcaaacacgg cgatcggctc ggccgtttca aagccgtttt
     3421 ccaccagagc cagggctccc ttccaggccc gaagtccttt ggagggaaga agcagggttt
     3481 tccacttctg aaccccgcgc gtcccgaaga tcttcacgac ggcgtcccgg tccggggaaa
     3541 gacggacggc gatgacttga tttcgtccgg cctggagcgt ttcagcctcg ggcgagcgga
     3601 gaaatcccgg aaaatcctcc agagcggcgg ccgccgcctc tccttcccgg gaggccgttc
     3661 ggaagccgcg gtagggcggg acggcgagcg ggcggatttc catcaccggc ctcctcccgg
     3721 cgccggcgga gaaagggcga aagccggccc gaaatgatcg gccgcgaagc cggcgaggcg
     3781 gcgggccacg gcctccgggg tgaactcgcg gcggacgagg gaaaagccgg cccgggcgat
     3841 ccgctcccgt cggccgggat ctcgggccaa ctcgagaacg gcccgggcca gggcttccgg
     3901 atccggtttg tcgcataaaa cgatgttttt gtcgtgggcg aagaattcct cgacggccgg
     3961 agtcctgaga gtgacgaccg gacgggccag gcccatcgcc tggaagattt tatggggaac
     4021 gacccgccgc gttttcgcgg tccggccgaa gatccccagg caaacgtccg ccgcagccgc
     4081 cgcccgggcg agttccgtca tgggcacgcg ccccgagaaa tcgatgttgg ctaaatccgc
     4141 ggccgccgcc cgggtcgcag ccgacgtccg cccctctccg atcagccgga agcggatcga
     4201 ccgatcccgc agccaaacca aacgggccgc ctcgacaatg atctcgactc cgtgaagggg
     4261 cagaaacgag ccgaaaaaga ggacctgaaa accgttgccg ggccggagcg cccgggagag
     4321 gagttccgtc tccgccggcg ggcggaaaac gtcgtcgtcg tagcccaggg gcacaacctc
     4381 gaccttggcc ggcgaaaggc cgtaggtttc gcaataatac ctcttatggg cggtcgtgtc
     4441 ggccaagacg agatcggcca aggcgaagga tgccctgtcg atctggaaat tccaccaggc
     4501 cccgggcgag tccgggcgcc cccagcccca gtcaatgact ttcgtctcgt agcgggcggc
     4561 gagcggatcg aaaatcaacg gccgggcggt caaagaggcg agcaaccggg ccggggggac
     4621 gtccttggcg cagaactccg ggacgaagag acagcggccg gccgccgccc cctccggccg
     4681 gagaaggccc cgacggaacg catccaggag aagcagcggg tgacgggccc aggatttcag
     4741 ccccgcccca aagcggcatt cgacgacttc ggctccaatc cggccgagac ccttgcggag
     4801 aaccgcgccg cgcgggtagc ccggaagaaa accgccgaaa aattcaaaac gcacggacct
     4861 gttttattcc acggcttcgg ctcttgtcaa tcgggaaggc gcttcttaag gctttatctc
     4921 gatttcggat cggggcagcc gaagccggga ggaaggaggc gggattagag ggaagcgacc
     4981 ctgaacgatt gacggcgatc gagcggtggc gtatccttac ggcagcggag atcatccgca
     5041 ggagtccgtc atggccctcg actcttttct cctcgacatt ctcgcctgcc cggaatgcca
     5101 gggcgacgtc aagatcacgg ccgatgaaca aggcctcaag tgcctccgct gccaccgggt
     5161 ctatccgatc cgggacgaca ttccggtgat gctgatcgag gaagcggcga tcgaacccgg
     5221 gcgggccgtc aaagagtgat ttgaatcccc gctccctcct ccttctccgt ttccgccgtc
     5281 tcggcgacat cgttctgacg acgccggccg tctccctcct caagagccgg tttcccggct
     5341 cctccctgac ctatctcgtc gaagagccgt accgccgctt ggtcgagggc catcccgaca
     5401 tcgaccgggt catcgtcgtc ccggccaagc agaaccggtc cgatttcctc cgcctcatcc
     5461 gggagatccg ccgggagcgg tacgacgcgc tcctggattt tcacggtggt ccccgggccg
     5521 cctggatcgc gctcttggga ggcgcccgtc tcaagatcgg ccacgccatc aagtccaagg
     5581 gctttctcta ccaccggacg acgccccggc gcggtctcga ttcgcccctt cattccgtcg
     5641 aaacccacgc cgggctcgtc cgggccttgg gcgcggattt ccggaaagag gacatccctc
     5701 ccttgatcct tccccccgcc cggccggagg aaatcgcccg ggtcgaggcc ttgaccgcgg
     5761 ccgtcgcctc cggacaagcc ggaccgggcg gcggccccct ccccctcgtc gttcttcaca
     5821 tcggcgccgg caacgccttc cgggactggg gcgaggaaaa cgcggccgtc ctggcttccc
     5881 gtctcctcga aattccgggg atctccctcg ccgctgtcgg cggagaaggc gaccggccgc
     5941 gggaggaccg gatcgccgcc cgcttgggct cgccccgatt cctttccttg tccgggcgct
     6001 tgaatatgat cgaggtgcgg gagcttgtcc gccgggcggt cctgttcatc ggtcccgaca
     6061 gcggcccgat gcacctggcg gccggtaccg ccacgccgat catcgcttac ttcggcccga
     6121 cgcttccggc cttgttcgga ccctggcggc cgggcctcaa cccggcgaaa acggtgattc
     6181 tcgagacgaa tccggactgc cggccctgcc gccagcgcgt ctgcgaaccg ggggattacc
     6241 ggtgcctgcg gaacatatct cccgacgaag tacttgccgc cgtccggccg tttcttcctt
     6301 cctagtccgc cctcccgcct tccctcccgg cggaggatcc ggccgcttga atccgtcctc
     6361 gtttcccctg agacgggcgt tcaattcgct gaaaataaat gctatactcc cccccatgat
     6421 gaaaatcatc cgtgatttcg tctccggacg cccggccggc gacatcgttc tcgcttccgc
     6481 cctggccgct gccctccttt tctccatgat ttccatcagc ttagcccaag gattcctggc
     6541 gatcgccctg atcgtctggc tcatccttct tgcccgggga aaacgcaggt tcgagacgcc
     6601 ggtcttcttt tgggccttgg cggcttacgc cggtctttcg ctcctttcct cggccgcttc
     6661 ggtcaaccct cggatcagct tttgggactc gcgggatctc ctgctcatcc tggccgtccc
     6721 ggtcaccctc gcggccgtcc gccgccgggc cgacctcacg ctcgctctgt cggctttatt
     6781 tttatcggcg gccgtcaacg gcgtttacgc cgtcttctgc cgctttgcga cgacggccga
     6841 gcggatccgg gcgttcatgg gtcattacat gacccaggcc ggcctcacgg ccttgttcat
     6901 ctccctggtt ctgggcttgc tgttcgccgg attcggccgg agaaaaagcg cggccgccgg
     6961 gcgcgccgag gtcaagggaa tccggatcga gcgggccgtt ctggcggccg gttttcttct
     7021 ggcctcggcg gccaatattc tgacatacac ccggggtgct tggatcgggc tggcggccgc
     7081 cctggccgtc gccgtctttc tctggaaacc caaggccttg attctcctgc ccgtgatcgc
     7141 cgccctcgga ttcctggccg cccccaagcc ggtcaaagac cgcgccctga gcattttcac
     7201 gatgcgcgac gattcgaatg tcgcccgcgt ccagtatttc aaggccgggg ttaagatcat
     7261 ctccgattac cccctcctgg gaaccggacc ggacaccgtc gacatggtct tccaggacga
     7321 aaagtacggg ctcggctact tggcgaaaca caatgtccat ctgcacagca acatcattca
     7381 gatcgcggcc gaacgcggcc tggtcacgct ggccgcctgg ctcgcgttca tcggcctggc
     7441 cctggcgggc ctcgggcgcc tttggcgcct ccccgagacc ggcttctcct tttgggccgc
     7501 ccggcggaag ctcgccgccg gcggcctggc cgccctgacc tctctctttg tcgccggatt
     7561 tttcgaatac aacttcggcg actcggaaat cgcccagctc cttttcattt tgatcaccct
     7621 gcctttcgcc gcccgacggc tcgaacggga atcccatgaa tccgcgccgt cttgaaaaga
     7681 aaagacgatg gggccgcctc cttcggatcg tcctcgggac ggcgttcgcc gtctatctcg
     7741 cctggctggc tcttgaaatc ctgtcgtatc ggaaatacga ggcgccgccg aagcccgccc
     7801 ggaccgagag gacgatggtt ccgcccggcg gcccggcgga ggcctctccc ctttccgccg
     7861 cccccccgga acccgtcgag ctccgcggcg cttatcacct ccattcccgg cattccgatg
     7921 gatcgaagac ccccgccgag ctggccgcgg cggccgaacg cgcgggcttg gatttcatca
     7981 ttctcaccga ccacggcgtt cccaatctcg cttccctcga ggcccagggg cgcgccggcc
     8041 gggtcctggt tatcgccggt accgaaatct cctccagccg cggtcattta gtcgccctgg
     8101 gctttgaccc gcccgcccgg ccgttctccc aggaagccgg ggcggccgcc cgcgaagtcc
     8161 atgcccaagg cggattcacc gtcatcgccc atccctattc caaaacccgc tggtcctggg
     8221 gaagcgcgga ggacgccgac gggctcgaga tcatcaatct tgacggcgat cttcgccggg
     8281 gatggggcac cgccctgttt tacgcccccg tccttctcgt caaacccacg ctggcccttc
     8341 tgaaaatcct ggatcctccg acggccgcgg ccggtaagtg ggaccggatt ctggccgaaa
     8401 ccgaagagcg cctcgatcct cccgccgccg gatattacag cctcgatacg caccgtgttc
     8461 tccacgagac ggccttcggc gttctcaacc tccacatcca gcttgattcc ctcatccccg
     8521 ccgacgatgc cgcggcgtcc cgggccgtgc ttgactccct ccgccgcggc cggttttaca
     8581 acgccgtcga cgccgccgcc gatccggcgg gcttccggtt ctgggccgag ggcgggccga
     8641 cggctcccgc gactctccac gtccgcgccc cttttccctt cgcccatgaa atccgcctcc
     8701 tgcggcgggg agagccggtc gccgccggaa ccgggccgga gctcgtcttc aacgcccggg
     8761 aaccgggcgc ctaccgggtc gaagtgcatc ttcgggcccg gacgccgctc cctcgcggcg
     8821 ttccctggat ttgcagcaat cccatcattg ttcgaaagga agtcccatga ccacgatttc
     8881 tctgtccctt cccaaggccc gccgggccgt tctcgggttc cgcaatcgcc gggtgctcgt
     8941 cctcggcgac ctgatgctcg acaaatacat ttggggcagc gtttcccgca tatccccgga
     9001 ggcgcccgtg ccggtggttg aagtccagag aagcaccacc gccctgggcg gggcgggcaa
     9061 cgtcgcccac aacctggaag ccctgaaagc ggcccccctg ctggtcggcg tcgtcgggga
     9121 cgacgaggag gggcagtgga tcaagaaaca cgttccggac ggacggggga tctttctcga
     9181 ccctgaacgg ccgacgacgg tcaaaacccg gatcatcgcc cagcaccagc aggtcgtccg
     9241 ggtggaccag gaaaaaaagc aggccattcc cgtggggatg gaggaccgga tcgtccggct
     9301 catcaaaaag gaaaaatacg ccgggatcat catctccgat tactgcaagg gaatcgtcac
     9361 ccggcagatg atgacccggg tgctggccca cgcgacggaa gcccgcatcc ccgttttcgt
     9421 cgatcccaag gtcgagaatt tctccctctt ttcgccggtg acgctcatca cccccaacca
     9481 tcatgaggcc gagcgggtcg tccgccattc ctgccgaacg gacgaggagg tcgaacaggc
     9541 cggccgggaa atcatggaga tgatttcctg cctttacctg atcctgaagc ggagcgagca
     9601 gggaatgtcg attttcgagc ggggaaagaa gcctgtccac atcccgacga tcgcccgcga
     9661 agtgttcgat gtgaccgggg ccggggatac ggtcatcgcc acggccgccc tggccatgct
     9721 cggcgggttg tcgatccggg aagcggcggt catggccaac gccgcggccg gcgtcgtcgt
     9781 cggcaagatc ggcacggcga cctgcgcccc cgaggagctc ttagccgccg tcgaagacca
     9841 ttaaagcccg tcgaagcgga agttccgttc ggtcaacgga tcatcttggc tctccagtcg
     9901 ttgagaagat cggagagcgt tgtttccagg gggatgcggg ggcgccaccc ggtctcccgg
     9961 gtgatcttgg cgtggcttcc ggaaagaagc gggatatcgg acctgcggac ccgggccggg
    10021 tcgacctcca cgacgatctt ctcccgggaa tcccgaagca gcatcttgag gatctcccgc
    10081 aggggacggg ctttgcctga acaaatgttg tacgcttcgc cgggccgtcc tttctcgatg
    10141 agcagggtgt aagcccggac gacgtcccgt acgtcgctgt agtcgcgccg gacggaggta
    10201 tttccgacct tgaggaccct cgagggtccg cggccgctct tttttcccgt cgccgccgct
    10261 tcgatgacgg cgatttgacg agcccaatcc gagcagacga aatccgggct ctgccccggg
    10321 ccggtatggg tgaacggccg ggcgatgacg acggccgttt tttccgcccg ggcgtaaagc
    10381 gcggccaggg cttcggcgct cagcttcgtg aaaccgtacg ggctggccgc gttcaacgga
    10441 aatgtttccc gaagcggcat cctccgggac ggctggtttc cgtagacatc cgaggagctg
    10501 atgatcagga gtcgggcttc cggagcccga agccgggccg cctcgaggag gtggaacgtc
    10561 cccatcaggt tcgtctcgat cgtttcccgc cgctggttcc aggaagcccg gacctgggag
    10621 atggcggcca agtggaaaat ccggtcgggc cggcaggcgg ccacggcggc cgaaacggcg
    10681 gtcgcgtcgc ggagatcaag cggaaggagg gtcacccggg gggccgcggc ccggaaatca
    10741 gggcattcct ccgagcgctc gggataacac gtgccggtga tttggagccg ggggcggcgc
    10801 cccgcctttt cctccgcctc gagacgggtg atgagatggc ggccgacgaa acccgtcgct
    10861 ccggtgatga gaacgcggag ggttttccga ggtcgcggcg aagcggcgga cgtcatgacg
    10921 tttcccggat cgaggaaaga gctcagaggg cggcccgcca gtattcgagg agatcccgca
    10981 gagtcgtctc gaaggtgatt ttcggagacc agccggtggc cgccttgaac ttggagacgt
    11041 cggcgcacaa gatctggaca tcggacgggc gcaggcggtc cggatctatc cggacctcga
    11101 ctttcgccgg gctgagagcg agcagccggt cgagcaagtc cttcatcgag acggcgttcc
    11161 ccgtgccgat gttgtagacc tcgccggacg cgcctttctc caaggccagc cagtacccgc
    11221 gcaccgtgtc ccgcacgtcg gtgaaatcgc gcttggcctc gaggttgccg acgtggatga
    11281 ccggagggac gaggcccttc tcgatcatca cgatctggcg ggcgaaattg gaggtgacga
    11341 acacctcgcc gcggcggggt ccggtatggt tgaagccgcg cgtccggacg acgcggagtc
    11401 ggtagctttg ccagtactga aagcccaaga ggtcctgggc gaccttgctg accgcgtacg
    11461 ggctgagcgg ccggagcgga ttggtttcct tcatcgggac ttcatcgggg tggaccagcc
    11521 cgtattcctc gctcgagccg gcgatctgga tccggggatc gagcttctcg gccatgaccg
    11581 cctcgaacag gttgatctgc ccgacggcgt tgatcatgaa cgtctcggcc ggaagcttcc
    11641 aggaggccgg gacgaagctc tgggcggcca ggtggaagat ccggtccggg cgggcggcgg
    11701 ccaacgcttt ccgcagggag ctcatgtcct tgaggtcggc ttcataaagg gtgattttcc
    11761 ccgtatggag gagcggggcg atgttctcca tccggctccg ccagcggacg aggccgtgga
    11821 ccttgacgcc gggatgatgg gccaggatgt actcggccaa gtggctgccc gcgaaaccgg
    11881 tgattcccgt gatcagaatg ttcatgtgat gttcaactcc atctggccgg aagacgcctt
    11941 ccggcgctgg tcttcgatcg attgaagcat ttcgggggtg acgtcccccg tgatgtaatt
    12001 tccgtcgaaa caggccgcgc agaaccggcc gaggttcgga ttttccatcc gaatggcctt
    12061 gaccaggttc tcctgcgttt gatagatgac ccggtccgcc ccgatccgga gggcgacctc
    12121 ctcctcgttg cggtcgcggg cgacgaactc cttccgggtc tgcatgtcga cgccgtagac
    12181 gcagggatag cgaagcggcg gggagcagga ggcgaaatag acgttccggg ctccgcagtc
    12241 ccggacaaga ccgacgatgg cccgggcggt gttgccccgg acaatgctgt cgtcgaccaa
    12301 taggacgttg cggtccttga attccgcggc gatcggattg agcttgaacc gaaccatgtt
    12361 ctgccgatcg ttgacggcgg gcatgatgaa cgtccggccg atgtagcggt tcttcaccag
    12421 ggcctcgcgg tatttcaggt tgagctttcg ggcgatctcg atggccgcgt cccgggccga
    12481 gtcggggatc gggacgacga tgtcgatcgg cagcccggct ttgcggatct cctcggccag
    12541 gagctcgccg aggtggacgc gggcccggta aacattgacg ccgtcgatga tcgaatcggg
    12601 ccgggcgaag taaacccatt cgaacaagca gggcgaatgg ggcttgtccc agatccgctt
    12661 catgtggatt tcccggttgt gatcgaagaa gatcgccgag ccgggaggaa tatcgcggat
    12721 gtcgctgaaa ttcatgatgt tgagagacac gctctccgag gcgatcgcgt aggacgggac
    12781 gacgccgtcg cggcgctggc cccaggacag cgacttgatc ccgaagggat cgcggaaggc
    12841 cgccatcccc tggccggcga tgtaggcgac gacggagtag ccgccgatga cctgtcgaaa
    12901 gacggactcg acggcccgaa agacgatctc aggggtcaag gaatcgggat tctgggcttc
    12961 cagggcgacg gcgaacacgt tcaggatgct ctcgacgtca ttgtccgaat tgaggaggcg
    13021 atggtgcctc tcggcaaggt gctttttaag ggcgacgtaa ttcacgacgt tgccgttatg
    13081 ggccatcatg aggccgaagg gggcgttcag ctggaagggt tgggcgtcct cgccccggcc
    13141 gccgccgatc gtcgggtagc gggtgtggcc gattccgatg gccccggtca gccgctcggc
    13201 gtgggcggct ttgaagatgt cgcggaccag cccgttgccc ttcttggtgt gaaaacggcc
    13261 gtcataggtg atgatcccgg ccgcgtcctg gccccggtgc tggatcgcca gcaatccctg
    13321 gtaaaggtcc cggaagacct gtccgttggc ccagataccg ataacgccgc acatggatcg
    13381 acccgcctgg aattatatca aatccgccga cttatttccg gtctttataa tagctctcgt
    13441 agaaggattt gaaggcggcg tttttttcct tgatcctcgt ccaccaggcc cggttatcca
    13501 tgtaccaccg cacggtcgcc tcgagccctt cctcgaaatc gaccttccgc ttccatctca
    13561 aagccttgat tttattgatg ttgacggcat accgggtgtc gtgcccaagc cggtcgggaa
    13621 cgaacttgat caggttcttg gaagcccccg tgagcttgac gatcctccgg gcgacctcga
    13681 tgttcgtccg ctcgtcgttg gctccgacgt tgtagacctc cccgagccgg ccccgccgga
    13741 tgaggcattc gatcgcccgg cagttgtcct cgacgtgcag ccagtcgcgg acgttccggc
    13801 ccttcccgta caggggcagc gttcgtccct ccaaggcgtg ggtggtgaac agcgggatga
    13861 atttttcggg atgctgatac ggaccgtagt tgttgctcgg gcggaggatg atcgtcggcg
    13921 cgccgtacgt cgtgtgatag gcctggacca tccggtcggc tccgaccttg ctggcggcgt
    13981 agggcgagga gggattcaag gaatccgtct cccggaaaaa gcctttctcc cggctgccgt
    14041 agacctcatc ggtcgagatg tggacgaaga attcgacgcc gggcgctttg cgcagggctt
    14101 caagcaaaat atacgtcccg aaaacgtccg tcaggacaaa ttcccccgcg tcgacgatcg
    14161 accggtcgac atgggtttcg gcggcgaagt ggacgacgcc ctggacgcgt tcgaaacaac
    14221 cggcaacgag ggccgcatcg cggatgtcgc cgtggatgaa ttcgtgtcgc ggatcgcatg
    14281 aaacgtcggc caggttttcc aggtttccgg cgtaggtcag cttgtcgaga ttgatcacgc
    14341 ggagcccggg atattcggcc agcatcatcc ggatgaagtt gctgccgatg aaaccggctc
    14401 cgccggtgac gagaatcgtt ctcccgttca aggcgcgctc gagaacggag ggagccgctt
    14461 tgagcccgga cgcccggcgg ccggccgacg gaacggcttt tttcacggga cggcgtttcg
    14521 tctgcttcat cttcttctct ccacgggtct cgttttggat tccatcccgc cggcgacggg
    14581 acgaggattc acggcaacga cgggaatcaa acgaattccg tctcagccgt ccttccgctc
    14641 ccattcatac ggtatctctc cggaatgggg atcgagccgc tgctcgtccg gctccttgta
    14701 gttgtacggc tcggtcggga cattgacgac gatcgcttct tccgggctcg tgcacatcca
    14761 gccatggtaa acgccggccg gaatatggat gaggaggggg ttgtggacac ccatgtaaaa
    14821 ctggttgatc tcgcccttgg tcggcgagcc ttcccgggcg tcgcagaggg cgaccttgac
    14881 catcccgtgg acgcaggcca tgttgtcggc ctgcctctcg tgcttgtgcc aggccttgat
    14941 gacgccggga taggtcgtcg tcatgtaggc ctggccgaac ttctcgaaac actcgtcgtc
    15001 ggcccggaag atctccatca gccggccccg ctcgtcgggg atgacccgga gcttttttac
    15061 gctcacgcct tgaatcatgg cttctttcct cccatcggct cgcgggacaa ccggagcagg
    15121 tactcgccgt aggcgttcgg gatggcctcg gccaaacggc gcagtctctt aacgtcgata
    15181 tagcccaggc gaaaggcgac ctcctcggga caggagatca tcaggccttg ccggtcctcg
    15241 accgcttgga cgaaattggc cgcctgaaga agagattcat gggtcccggc gtcgagccag
    15301 gccgtgcccc gccccatctc ctcgacccgg agctcgcccc gctgcaggta gacgagatta
    15361 aggtcggtga tctcgatttc gccccgggcc gagggcttga ggccggcggc gaaatttgaa
    15421 gcccggccgt cgtagaaata aagcccggga acggcgaaat gcgaccgggg cttggccggc
    15481 ttctcctcga tgctgacggc ccggccccgg ctgtctaact cgatgacgcc gtagcgctcg
    15541 ggatccttca ccgggtaggc gaaaacgatc gcgccggtct tcagccgggc gcatttcctg
    15601 agctgtccgg gcaatccgtg gccgaaaaag atgttgtcgc cgaggatcag gcaggacggc
    15661 ttgccgccga tgaaatcgcg tccgacgaga aaggcgtcgg ccaggccccg cggctcactt
    15721 tgctcccgga actcgaaccg gagcccccat cgccgcccgt ccccgaggag agcccggaac
    15781 gcctcgagcg actccggcgc ggagatcacg aggatgtccc ggatcccggc cagcatcagc
    15841 agggacaggg gataataaat catcggcttg tcgtagaccg gcagaagctg cttgctgacc
    15901 gaatgcgtca gcggatggag gcgggtgcct ttgcctccgg ccagaatgat tcccctcatc
    15961 ggattgtcct ttgcccggga ttcgcccgaa aatataacat agattgacag ttttttccaa
    16021 aacgcataat ctgtccatag tcatgaaaaa cggggcggaa cgaccgagtg gataatccaa
    16081 gccatcgacg tcttcttcgc ggactcgcgg tcattttata ttccttgctg ctggctcttt
    16141 tctattggaa gtatgtgccc cttgtcgccg cttaccaggc cgttcttatt ccccttctcc
    16201 ttttcgtcgc cgtcgccgcg gcgaccgacg tccgaaaggg cttgctggga tttctttttc
    16261 tatttccgct gatcaacaat ctcccgtatt ttttcaagct ctacgatccg cttcccatgg
    16321 ccccctcgga cttggtcctg tttctctttt tcttcctggg ccggctgatc cacggggtct
    16381 tcttcccttt tccgccggaa gaaagcggaa aaggaccgaa ggaacctctc ttcacgccgc
    16441 tccgcctctt tgccggcctc gtccttttct ccgccctgat cacgttttgg cgctacacga
    16501 attttttccc cctctggcgg ccgcggattt atgagcttgt gacgaacgtc aagggcgtca
    16561 gcgccggcgg ggcgatcatg agcgtcgtct tctacagcct gacgtacctg accggcatcg
    16621 tttttttcac cctttgcgtc cgggttctcc ggacggaggg gttcattaaa aaagccgtcg
    16681 cggcgtttgg ggtcggatca tttctctcga tcggtttcgg gtttattcaa aaatttcatt
    16741 cccccgccct cggcaatctt ccggccagtc tccgtcacgg ccttctcaac gcgaccttca
    16801 aggacgccct gtccttcggg gcttttctct caatgctcat tcccttcttg gccggactcc
    16861 tgcccgccgt caagggaagg acgaggctga cggcggccgt catgacggcc gcggccggct
    16921 atttcttgtt ccacgccggc tccaagagcg ccctcatcgc cgccgccgcc gcccttctct
    16981 tcctgggaat cctcatgctt ccggcgattc tcgagcggcg cggtccgtta gcccgcctgg
    17041 tcatccctct ggggctggcg gccgccgtat tcgccggcgc ctcgatctat ctcttgatat
    17101 cgggaaattt cccctccgag aacggacagc gcaccgcgac catcgaacgc atggaggatt
    17161 tcgcctcagc cctcgaaatc cgcaccgccg gccactggag gatcgcgggc cagatgatcc
    17221 gggattatcc cctaagcggc gtcggggtcg gggcctatat catcgaaatg gccaacacgg
    17281 cgaaaaccct caagatctcg atcggagttc ccgaatcggc cgaaaacctt cctctccacg
    17341 tcgggtccga actggggatt cccggcatgc ttctattcct ttggatcctt tgggagatgg
    17401 gaaggcggat tttcctctcg ttccgggcgg cgccttccgg gacgttcccc agggccctca
    17461 tcgccgcctc ggccgccgga ttgttcgcct tccttgtgaa ttcccagtcg cattcctaca
    17521 tcggaagcta tgaaatccag tacctcttct ggtttctcgg ctccctggcg ttcgccgggg
    17581 acgtcccttc gcccgccgcc gcggcagggc ggaagccgtc gagccggcgc ttgggacgcc
    17641 tcttcggaac aatcctcctt tttctcggca cggcggggct tctttggaat tcagctcatt
    17701 cgctctcgct caacgcccgc accgagcgct accggctccg gcagggattc ggcctcggtc
    17761 cctatgaaaa ggacgcggac ggacggcgtt tccgctggag cggacgatat gcgggactga
    17821 ccggccgggc cggcgctcct ccgctcaagg cgtggctcct cgcttctcat ccggacatcc
    17881 gcgtccggcc ggtccggctg cgcctcttcg ccgccgccga cctggccacg aaaccggtcc
    17941 ttctccaaga cctcgtcctt aaagaaaacc tatggaaaag gatcgagctc caggcccccc
    18001 ccggcttccc ggaatcttcc ctttttctgt tcgaggtgga tcggacgtgg aatccgcgta
    18061 aggcgggcgt atcggacgat ccccgggacc tcggcctggc gatcgggatc atccaggcgg
    18121 ccggcccgat caagccgaag gaaaaaaccg aggcggccaa gcccgcaaac agtcctccgg
    18181 aaaagaagga aaacagttag ggagaggtaa gggggattct ccgcgcctcc ggtccgccgg
    18241 acgcgcggct caccaatgat tgatcgagct cgtgtccggg cccgggacgt aggtgggcac
    18301 gatttcccgt aaaagaggaa tgatctcgga tttccggcag gaccccgatt cgcaggcatc
    18361 gatcagccgg tccacgcttt tccagatatg ggcggcattc aagggtttcg aattcctggc
    18421 caggaagatt tttcggtgtt ccgtgggcat cgccccttcc tcggccatga tcagctcttc
    18481 gtaaagcttt tcgccggccc gcagtcccgt gtagacgatc atgatgtcga catccggctc
    18541 caatccgctc agccggatca tctcccgggc gagttcatcg atggacacga gctcccccat
    18601 gtccaagacg aacgcctcgc cgcctttccc cgccgtcgcc gcctcgagga cgagaaggac
    18661 cgcctccgag acggccatga agtaccgctt catgtcggcg tgggtgacgg tgaccggtcc
    18721 gcctttacgg atctgctctt tgaacagcgg gataacgctt cccctgcttc ccaggacatt
    18781 gccgaaccgg acggagatga accgggttcc gcccatcgcg ttcagtgccc gcaggatttc
    18841 ctccccggcc cgcttcgaag cccccatgac gctcgtcggg ttgatcgcct tgtcggtcga
    18901 gatgttcacg aaccgttccg ccccgtaccg catggccgct tcgccgagaa tccgggtccc
    18961 ccggatattc gtcttgaccg cttcctcggg gtagtgctcc agaatcggga cgtgtttgag
    19021 ggcggccgcg tggaggacga tttccggccg cgtctgctcg aagatccgat cgatcttggc
    19081 ttcatcgcgg atcgtcgcga ccaccgggag gaccccggcg acggcgtttt tcagcctgtt
    19141 cacgacgtag aaaagctcgg tctcgtcgtt atcgagaagg atcagctcgc ccggctcgaa
    19201 ttggctgatc gtccgggcca gctcgccgcc gatcgatccc cccgccccgg aaacgagcac
    19261 gcgcttgttc ttgagaaacg cccggattcc ctcaaagttg atttgaacgg gcgcccggcc
    19321 aaggaggtcc tcgacctcca cgtccttgat gtccatcagg gatactttcc cctccacgat
    19381 gtcgtggatg ccgggaagga gcttgatgct ccggacttcc cccgtcgagc ggaccagctc
    19441 gacgattttc cgaatgtcct tcgaattcac cgtcggaatg gcgatcagaa cctcgtcgac
    19501 gccgttgccg tgaaggattt gggggatatc ctgtcgtttt cccaggactt tcaccccatg
    19561 gatctccgtt ccccgcttcg cttcgtcgtc gtcgacgaat ccgattggga aatgggccga
    19621 tttccggttt cccgtcattt cccgggcgat ctgctctccg gccgtccccg ccccgacgat
    19681 caggactttt ttccgacccc ggctcaagcc tcttttgcgg aaatggtact ccctcaaggc
    19741 gcgcttggat atccggagca tcccgagcag gaccagggtg aaattgtagt tgaggagaag
    19801 aaccgaacgg gggatttccc tcattccggc cagccaattg aacgcataaa tgatgacgat
    19861 cagcaaaaga taggacaagg tcacggcctg caggagcttc agcatgtcct tcaggccgaa
    19921 gaatctccag gcgacattgt agaggccgtg gagggcgatc atcgtgatcg tcacgaccac
    19981 gacgacgcag atcctcaccc acaggccttt ggtgaacgtc gccgggatgc ggccgtcgaa
    20041 gcgaagccaa aaggaaagat aaacggacag ggcgatcagg acggcgtcgg cggttaagaa
    20101 aaaaaccttc cttttgagca ccgtgggccg gaaaaccggc gcgatttttg attcgaaacg
    20161 ggcggtttcg ctttttccca ttccttcgtc acgtcctttt caggggacgg taaacaatat
    20221 actctttcgg cccgggcatt tcaaaccgcc tcttccccgc cccgggcgga aagaatctct
    20281 ttcagggacc ggcagatgta cgccagctcc tccagccgca agtccggata gatggggagg
    20341 gaaaggcagc cgttccagat ccgctccgaa ttcggatagt cggccggatt cagaccgtac
    20401 ctttcccggt aatacgtcat ccggtacaag ggtttataat ggacggacgt cccgatgccg
    20461 cgatcggcga gccgctccac gcaccggtcc cgggagagcg gggctttcgg cgtgagaacg
    20521 accggaaaca agtgccaggc atgatcctcg aatccgacct ggagctttgg cagatcgagc
    20581 cccgggattc cggccagcgc ttccatgtaa tagcgggcgc agcgttcgcg ccctttccgc
    20641 agcgcctcgg cccgctccaa ttgagccaag ccgatggccg cggagacgtc gggcaggttg
    20701 tatttgaagc cgggggcgac cacgtcgtac tcccaggacg gcttgtcgga cacgtagcgg
    20761 ttccaaatat cgcggtcgat gccgtgaagc cgcatcactt tcgcccgggc ggcgacgcga
    20821 tcgtcggccg tgaccagcat cccgccctcg gccgtggtga tcgtcttgtt ggcgtagaag
    20881 ctgaaacacg tgacgtcgcc gaaggttcca accaggcggc cgccgcgccg cgagggaaag
    20941 gcatgggccg cgtcctcgac gacgcggatt cccttccgcc ggcagacgtc gaggatcccc
    21001 gcccctttct caacggtcat agccgccgcc tggccgccgt aatgtacgac caccaccgtc
    21061 ttcacttcag ggaagcgccg gacggcttct tccaggatct ccgtcgtcaa aagcgaagtt
    21121 ccgtattcaa catccaggaa gacgggatca gcattaaggt aacggatgac ctcggccgaa
    21181 gcggtgaacg tcatcgccgg gacgaagacc tttgagccgg gcccaacgcc gatcgcttcc
    21241 agggccaggt ggagggcggc cgtgccggaa ttgacggcgc aggcatggcg ggccccgacc
    21301 gccgccccga actttttctc gaattccagg gtcttggaag ccgtcgtcag ccagccgctt
    21361 tcgaggacct cccggacgta ggccaactcg ttcccgtcgc acaaaacgcg gctgtaaggg
    21421 attttcatct cagccatgcc ggcacctgcc ttccttcaga acttgaggac ggccgcgatc
    21481 gttttaaaga tcaggccgag gtcgcggccg aagcggggcg ggccctcggc gtatcgctcg
    21541 tagagatcca gcttgcgggg aaggatctcg tttcggtaga cggcttccgg atcggccgcc
    21601 gccgccagca gctcctcctc gttccggtag atgatggacg ccggatcggt gatccccggc
    21661 ttgacccggt ggacgaccgc ccaccgccgc ggataggccg ccacccactt cgacacctcg
    21721 ggccggggac cgaccatcga catgtccccc ttcagcacgt tgaacagctg gggccactcg
    21781 tcgattttgg tcttgcgaag aaaccggccg aaggatgtca cccggcggga atcgccgggc
    21841 tcgaatccgc cgccctcgcc ttccgaggcc gggatcatcg ttcgaaactt gaaaagcgaa
    21901 aacggccgcc ccgcccttcc gatccgcgtt tggcggaaaa ggaccggccg gccgtcggcg
    21961 agaaaaatca agacggccac gagcaggaaa accggcgaaa gcaaaagcag ccccagcccg
    22021 gacgccccga ggtcaaacgc tcttttcatc actcgccgcg gccgaccgaa gaccgagctc
    22081 cccggcaaag gcctcatcca aataggcgac atagaagacc gtgtcctcgg tttccaattc
    22141 cggcgtttcg atccatcggc ccttgataaa aaccctgcgg ctcggctggt tgatcttgta
    22201 aattcccgcc cgaatcgccc ggcttccccg tcttcggctg taggttttcg tctcctcgac
    22261 cagagccggg aaaatgcccc gaccgcggca attcttgtcg acgaccccga tcgtcatctg
    22321 ggcgaatccg ggctggtcgg cgatttttct gccggcgctc gtcacatccg gccggcggaa
    22381 aagacggctc cagagcttat gattgaaaag cagcgccggc ctccggacca ggctgacggc
    22441 gaacgcggga aggcaggcca gaaacatcgg ccgggtgaac gggccgtcgc agacggccac
    22501 aatcccgacg atttttcctt ctctctcggc gaccagggca taggacaccg cgctcgtcac
    22561 gagccagcgg tacatcgccc ggacataatg ccggccgagc atggccggga cgtgctcgcc
    22621 gaatttgaag gaatcgcaat ggagagccgt catatcctcc aagtcccccc gcccggccag
    22681 ccgaataacg ggatcgtcgg aatgagtcat cgtccccctt cctttctccg cggccggacg
    22741 gccgcctctt cgaataccgc cgtcatccgg ccgacgccga tggaaaacgc cagctccttc
    22801 cggtagaact cggcgttttt tctccccatc atctcccgct cattccagga aagggaggcc
    22861 agccccagga ccgcctcggc cagggcggcc ggatcttcgg ggcggcatgt ccaggctcct
    22921 ccggcccggg ccgccagctc ggccgcgctt ccccggatcg ccaggagaac cggttttccc
    22981 gcggccaagg aggcttgggt tttctgggga atccccatcc gggccagggg atcgtccttc
    23041 agatgcacga gaagcacgtc ggcccgggcc gtgatccggc ccaggctctc cggcgcctgc
    23101 cggggaatga acaggacgtt tttctgcccg cggccggcgg cctttttctt caaggccgat
    23161 tcctccactc cgtctccgac aaaaacgaag cggatgtccg gacgtttttc ctcgatcaag
    23221 aaagcggcgt ctaagacgcc gcccaaggcc tggagcgctc cgaaattgcc ggaataaacg
    23281 acattgaacc ggccggcgag cccgaacgga tcggatccct ccccgtccgt ttcccccgga
    23341 agcgaccgtt cgtcgcacca attatagacg acctcgattt tcttctcggg aacgccccgg
    23401 gcctccaagc attttttgta tccgttcgaa agaacgatga ttttccgggc cagccggtag
    23461 gtcgccttgc tccaagcccc caaaacggct gaaacgccgg gaagagaaag cattccagtc
    23521 acggtcacgc tttccggcca catgtcctgg acgtcgagga cgtacggcgt cccgaacatc
    23581 atccggaaca ccatcgccgg ccaggccagc gtggccggcc cctggtagac atgaacgacg
    23641 tcggggcggg cgaccaacgg cggccccagc agggcggccg tgccggccaa actcagatat
    23701 cctgccgtcc gccggacggc cgaacggtcg tggctcggat acatcatgac gcgggtgacg
    23761 gggacgccgt cgaggatttc ccgcatccac ggccggatcc ggtagccggg atagactttc
    23821 ccggcgggat aatgggggaa tcccgtcaac acttcgacgt catggccgcg gcggaccaac
    23881 tcccgggcaa aggccaaacc cttcaaatgg ttcggctccg gctggaacag cggcgtcagg
    23941 agaagaacgc gcatcaatcg gccgcgcgcc acaccgtccg gttcacatag tccgtgtagc
    24001 tcaggacgat ccggaggact ttttcggaca cgttgggaac gtcataatcc gcgacgatcc
    24061 gcggtccccg gcactcgccg cgcggctggg tggccaggac ggccaagccg cggcggacgt
    24121 tttcgtaatc caagccggtc atcatcaccg ctccttcctc catcccctcg gggcgctcat
    24181 gggcttcccg gatgttgagg gccgggaagt tgaggatgga ggattcctcg gtgatcgtcc
    24241 cgctgtcgga aaggacggcg caggcctcga gctcgagctt cacgtaatcc gtgaatccga
    24301 acggcttttc cagcattatc ccaggggcca aggccggttt ccgggcctcg atgatttttc
    24361 gggtccgggg gtgggtggtc acgacgactt tcttttttcc gtctccggcc aggccgttga
    24421 ggattttcat cagcccatcg aacggcttgg acgaatcgac gttttcctcc cggtgggcgc
    24481 tgactacgta atactcgccg ggccggagct tcagccgctc gagaacgccg gatttcttaa
    24541 tcttgggaag gtaatgatgc aggacttcga acatcgggct gcccgtcttg atgacccggt
    24601 ccggcggaag cccctcgcgg aggaggtact ctcgggaaat cgcgctgtag ggaaggttga
    24661 cgtcggcgag gtggtcgacg atccgccggt tgatctcctc ggggacgcgc tggtcaaagc
    24721 accggttgcc cgcttccatg tggaaaatcg ggattttccg ccgcttggcc ggataagcgg
    24781 ccaggcagct gttcgtgtct cccaggacaa gcagcgcgtc cgggcggtcg cggtccagga
    24841 cggcgtccat ccgctcgatg atccgcccga tcgtttcgca gggggtcgcc ccggccgctt
    24901 ccaggaagcg gttcggcttg cggatctcga ggtcctcgaa aaagacctgg ttcagctcat
    24961 agtcgtaatt ctggccggta tggacgagga catgctcgca ttcccggtcc aaggccggaa
    25021 tcacccggga caggcggatg atctc
//
LOCUS       DAOOXH010000028        25012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_1794_length_25012_cov_6.783988, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000028 DAOOXH010000000
VERSION     DAOOXH010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 25012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25012
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1794_length_25012_cov_6.783988"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1776)
                     /gene="infB"
                     /locus_tag="PKV64_05005"
     CDS             complement(<1..>1776)
                     /gene="infB"
                     /locus_tag="PKV64_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPN16475.1"
                     /translation="PKKSAAAAKPAAPAAPAKAPVSAPSPGPKAAAPAPPAKAPVSAA
                     SPAPKPAAPRPRPQARLREGATIKDLADRFKIRPRDLADKLAVKGYNLAITDIISPSL
                     AASLAKDLNIVLDIVPVEESCRLEAAARPELLAHRPPVVTIMGHVDHGKTTLLDAIRK
                     SNLVDRESGGITQHIGAYQVTVNKKAITFIDTPGHEAFTQLRARGAKVTDIVILVVAA
                     EEGLMPQTREAINHAKAAGVPLLVAINKIDKPEANPDRIKRELQKDGLLVEEWGGDVI
                     SVEISAKERKNIPELLDMILLMADVLDLKANPSAPAQGVVLEARLDPRKGPMATVIIQ
                     NGTLRPGDTFLSGTTFGKVRAMTDDLGRTMPAAGPSSPVEILGYSDVPRAGDFLQVLP
                     DLETARRIAGQRKEAAAKAEPQRQEAVASLDDLFRKVETGAAKELPLIIRADVQGSIE
                     VLTGLLPSLGTDKVKIRIIQAATGSINESDIVLAQTTQAIIIGYNIKPSPKILETAGK
                     ENVEIRTYKIIYQLTDDIKKALTGLLEPVIKETFLGRAQVRKIFRIPKVGTIAGCYVL
                     EGKIARSAEIRVIRAGIAVHKGRLS"
     assembly_gap    1777..1876
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1877..2067)
                     /locus_tag="PKV64_05010"
     CDS             complement(<1877..2067)
                     /locus_tag="PKV64_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16476.1"
                     /translation="MSEKKTTKPKTKAAATPPASPPPPAVKPAAPAPPPSASKPPAAG
                     TAPKKSAAAAKPAAPAAPA"
     gene            complement(2088..3122)
                     /gene="nusA"
                     /locus_tag="PKV64_05015"
     CDS             complement(2088..3122)
                     /gene="nusA"
                     /locus_tag="PKV64_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPN16477.1"
                     /translation="MKINVWNTILQLSKERGVEPKVIVAAIEESLRVAAGRIYTRNED
                     VQITFKPEKGDLRVYCLKTVVEAPADAGREISLPEAQRLKADAAVGETIEIDLPSDTL
                     GRIAAQAAKQVLVQKVRDAESEKIYDAYADRIGEIVTGTYRRIENGALILDVNNLDVV
                     LPPWEMIPGETYDRGAQIRAVIVQVRRGQKGQETQIIVSRSSNEFLGRLLQTEIPEIS
                     SGIIEIKDIVRQPGERSKVAVISRERDVDPIGACIGPKGSRILAISKELGGEKIDIVL
                     WSPNPGAYAKSALNPARIERVIPVSTADKSLEALVSRDQLSLAIGKKGINVKLASKLT
                     GWKIAIKELG"
     gene            3329..5515
                     /locus_tag="PKV64_05020"
     CDS             3329..5515
                     /locus_tag="PKV64_05020"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPN16478.1"
                     /translation="MAAHLFWLAPLGSILALFFAWFFYHQMKKAPEGSERMAQIALYV
                     RQGAMAYLKQQYKVVTIVFVFLVIFLAILAFLFKVQNPWVPVAFLTGGFFSGLAGFFG
                     MKTATYAAARTTQAARRSLDSGLRIAFRSGAVMGLVVVGLALLDISLWFLILKAIYPV
                     NDGGHNLILITTTMITFGMGASTQALFARVGGGIYTKAADVGADLVGKVEADIPEDDP
                     RNPAVIADNVGDNVGDVAGMGADLYESYAGSILATAALGASAFLGTPDLQLKAVIAPM
                     IVAAVGTLLSILGIFLVRTKEGASQKQLLGALGRGVNGSSIGIVIVSFAVIRLLGLPL
                     GVWLAMIVGLASGIVIGRATEYFTSASYKPTRTVAENAKTGPATVIIAGLGVGMLSTT
                     IPVLAVVVGTILAFLFASNFDLNNVSHGLYGIGMAAVGMLSTLGITLATDAYGPIADN
                     AGGNAQMSGLEEEVRKRTDALDSLGNTTAATGKGFAIGSAALTALALLASYVEELKVG
                     LIRIGRTIIEFADGTTIDTARAGFMDFMRYYNVNLMNPIVLIGIFLGAMMAFMFCGLT
                     MTAVGRAASRMVDEVRRQFREIAGIMTGQAEPDYARCVSISTKGAQRAMLLPSLLAIA
                     LPVVTGLLLGVPGVMGLLIGGTSTGFVLAIFMANSGGAWDNAKKFIEEGHYGGKGSTA
                     HKAAVIGDTVGDPFKDTSGPSLNILIKLMSMVAIVMAGVTVVVHLL"
     gene            complement(5585..6916)
                     /gene="miaB"
                     /locus_tag="PKV64_05025"
     CDS             complement(5585..6916)
                     /gene="miaB"
                     /locus_tag="PKV64_05025"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPN16479.1"
                     /translation="MTAPGPPLGGLRFAVHTFGCQMNENDSERLAGFLRAAGASPAAS
                     VAEADLVVVNTCSVRAKSEEKLQSFLGRLRMLKKKRPLLVGVAGCVAQLRKESLAGPK
                     SGVDFVLGFDQYDRIAEVAAESARRRVVAVSRTRGWREKKTAPLRESAVSGYVTIMEG
                     CDNFCAYCVVPFTRGREKYRPVEAVLEEVRELAARGYREIQLLGQNVNHYRDPESGTD
                     FAALLGRAAAVPGPEWIRFLTSHPKSFPPALARTMAATPKICRQLHLPLQSGSASVLE
                     RMKRGYTRKDYLETAAALRALMPSIRLSADIIVGYPGETEAEFKETLSALAEIRFAGI
                     FSFCYSPRPRTAAARLTDDVPAEIKKRRLMEVQALQKDIQLAFHKTLIGGRERVLCTG
                     RSKKDPAVFAGRNEGNQVVNFRSNADVVGRFVEVEITDAGPYSLRGIAVAG"
     gene            complement(6913..7353)
                     /locus_tag="PKV64_05030"
     CDS             complement(6913..7353)
                     /locus_tag="PKV64_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HPN16480.1"
                     /translation="MSKPDAAGRLFQAGFSCSQAVFTPFAEDAGLDTETALKLSQALG
                     GGMAHLGFTCGAVTGAFLAIGLRHGRVRVEDSAAKEKTYAVMTEFARRFRARHGDDLS
                     CPPLIGCDLATAAGQKKARDGNLFQTRCATFVRDAAAILEDLLR"
     gene            complement(7432..9084)
                     /gene="recN"
                     /locus_tag="PKV64_05035"
     CDS             complement(7432..9084)
                     /gene="recN"
                     /locus_tag="PKV64_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPN16481.1"
                     /translation="MIKYLRLRNLATIEDIRIDWENGFSVLTGETGAGKSIIIDGIRL
                     VCGEKGSADLVRAGAAEASVEAIFDRPAGEEGEGDLILQRLLAAGGGKAYQDGILVPL
                     KRLKETTAGLIDVYGQNDHIFLLQIESHLAYLDQFAGTIPLREETARAARELRGLARQ
                     KEEWTARERERGQRLDFLEFQIAEIEKAGLRPGEEEELREKRLLLRNAEKIRGLLDAS
                     YDLAYGGEASLSAALVKLEHLLQELSPFHPDFREMKDSLSSFGITVRELSDLLLRFRD
                     RGEESPERLEEIEERLSLIEQFKRKYGAEIGEIQGYLDRLKAEREDFLQIKDRLLRVD
                     ADIVAAAAVYAASAQKLSKTRRTAARELSSLIEKEIALLGMKNARFEVRLDGRPFSPD
                     QPDSVREAGLDDVEFLISPNPGEPPKPLRKIASGGELSRIMLALKSAAREKDAASTLI
                     FDEIDAGIGGKTAEFVAQRLKALAARHQVLCITHLPQIASFAAHHYTITKKTEKDRTF
                     TTIRKLGPDQRIEEIARLMSGSLQTPASLAGARDMLERNAGP"
     gene            complement(9097..10431)
                     /locus_tag="PKV64_05040"
     CDS             complement(9097..10431)
                     /locus_tag="PKV64_05040"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HPN16482.1"
                     /translation="MTQRPAILRLLAAGVLIAWVLPMSAGLVGDVRPKGSAGPASGFD
                     PNGTYAPGEVLIKFRPEIPPAHRAAAMAVQRVRAIGRIAALDVYRALLPEGTAVEEKA
                     AALAADPRVLYAEPNYIGRASVTPNDNLFKYQYALSNIGQSIGDVPGSPQGKTGADIK
                     ATQGWEESRGDAAVTIAIVDSGVELTHPDLVGKIVGPGRDFVNDDYDASDDLYHGTAV
                     AGIAAASTDNGEGIAGVGWNAMILPVKVLNDQGTGTVSQVADGIVWAAGQGAQVINLS
                     LGFNDVSQVLFDAVAYASDQGCVLVAAAGNNGGPVQYPAAYNDYVLAVAATDYNDDVW
                     EGSNAGPEVDVAAPGVSILTAVPTWFLGSGPLPYSRFDGTSMAAPHVSGLAAILKSLK
                     PWLTAAQIREIIRLSADDVNAATLPGIDDSIGCGRINMAKALVPLKLEAGRP"
     gene            complement(10446..11237)
                     /locus_tag="PKV64_05045"
     CDS             complement(10446..11237)
                     /locus_tag="PKV64_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16483.1"
                     /translation="MPLRARSIAVLILGAAVLAGPAAAAAQEPAPFYRIHAGIEAEAG
                     SRMVVWDKDAGSSFLKSIRGRIRFGLELSGGASFSLLAGGGAENWNGLIFRRLPFSVD
                     YEAGSRGAIVLGAEMEVPLFRPGDWEIGAAAQALFSLGLKGEWTSEALQQPARLSGRA
                     SGTAVRLGPTLAYRGFETFTPTASVFYDGLWSSFTMEETIGSLSGSEKKKINGRGSVG
                     ASLGMIIEPSASFRLKAEGFLIPGKKLEGGWNLSPGAAARMEFLF"
     gene            complement(11248..12072)
                     /gene="bamD"
                     /locus_tag="PKV64_05050"
     CDS             complement(11248..12072)
                     /gene="bamD"
                     /locus_tag="PKV64_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPN16484.1"
                     /translation="MKKHKGAAAVLVLAAALALTPGCRKQQAVISPDIAGSDEALFKQ
                     GEQLMKKNPDKALILFRQVMESFPQSFYAQRAMLAIADTYFQKKDEGSLILAAAQYRS
                     FLGLYPTSPSAPLAQYRIGLCSYNKMLKPGRDQTKTEEALAEFKKTVSMFPLTEEAES
                     ARAKIQECENRLAEHIFVIGEHYYRKRSYRPAISRLTDVVMNYPLYPEMDKVYFILGD
                     SQYRQGKPDEARPYFTKVVTDYGNGKYASKAQKKLKEIEDAGRKKAAEKTPAPQKQ"
     gene            complement(12069..13049)
                     /locus_tag="PKV64_05055"
     CDS             complement(12069..13049)
                     /locus_tag="PKV64_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPN16485.1"
                     /translation="MKVLVTGAAGFIGSRLCRRLLDEGCDVCGLDCLTNYYPRWIKRR
                     NLRPLQADRRFTFIEADLNDLPLNRTLRPFEAVYHLAAQAGVRASWGRSFASYLHHNI
                     AATQRLLEAAKERPPRKIVYASSSSVYGLTPDLPMKETSPLRPISPYGVTKLSAEQLG
                     VLYHRNFGLPVVALRFFTVYGPGQRPDMAFHKFFKAILEGREIPVFGDGSQTRDFTYV
                     DDIVAALVSAERNGTAGEIYNIGGGHREKLSDLFPVFREITGRPVKTRRMEQQKGDAP
                     HTFAAIAKARRDLGYRPRTALRDGLEREWAYVLDLYDKRSRPATTQGSLS"
     gene            complement(13046..13714)
                     /gene="rpe"
                     /locus_tag="PKV64_05060"
     CDS             complement(13046..13714)
                     /gene="rpe"
                     /locus_tag="PKV64_05060"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPN16486.1"
                     /translation="MIQIAPSLLSADFSRLADAAHMVEKAGADLIHVDVMDGHFVPML
                     TFGPAHVAALKKVTALPLDVHLMVDNPSAMIPLFAEAGADWISIHVEAAPHLHRDVSA
                     IRRAGRKAGVVLNPATPLTALEAILPDADFVLLMSVNPGRGSQPFIEASHERIRLLRR
                     RIEERGLRTLVEIDGGVNLENFERLCEDGAQVFVAGNAVFGSPDPAGVIRTMRDIAAR
                     KERR"
     gene            complement(13716..14435)
                     /locus_tag="PKV64_05065"
     CDS             complement(13716..14435)
                     /locus_tag="PKV64_05065"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPN16487.1"
                     /translation="MASSTKANSILLFVLLYLFLFFASADIFSELIYRGELINLPELT
                     GKTVEEARALLAPKKISLATVDSRFDTRLEKGRILTQDPAKGSRVKPRRTVGVVVSLG
                     NEQIVVPRLEGRSVEMAAQLLKAAGLRRGRVSQIHTPQAAAGRILFQDPPAGTVVNRA
                     SAVDFLVSRGQEDPRYIMPDLIARNADSVLRQLRSLGFLKYDVSYAWYPDIGPGVILK
                     QSPVAGSLILKRNQIHFEVSR"
     gene            14605..15576
                     /locus_tag="PKV64_05070"
     CDS             14605..15576
                     /locus_tag="PKV64_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HPN16488.1"
                     /translation="MAEAEVGDDVIGFDPTVQKLEAMAAETMGKEAGLFCPSGTMGNS
                     IAVKAWTQELQEVIVESRSHIFNLESTHLTFISRVTPRPLPSRRGAMDPAEVEHFVKK
                     PNVHTPQTTLICLENTHNNWGGAVVPLENFKAIREVAVRHGLRVHLDGARIFNASTAS
                     GVPVRGYADEVDSVMFCLSKGLSAPIGSMLVGPKDFIEYGRRIRKALGGGMRQVGVIA
                     AAGLLALTRMTGRLAEDHRRARRLAEALSALPGIVLNPAEVETNIIIFKFEHPELTVP
                     GLLSELEKRGIRALAAPGGVGIRMVTHKDVDDADVDAAIGAFRDILG"
     gene            15621..17351
                     /locus_tag="PKV64_05075"
     CDS             15621..17351
                     /locus_tag="PKV64_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HPN16489.1"
                     /translation="MTKPKSARPAALIAAVCILLAAAAAAEARIVGPAEARTAAARLL
                     EFENARPDLRLTPGVFALGRVEPLLRRGRPVAYLARLEPRGFMILSDITEGSPQVFVS
                     YEGDPDRMARIPYLARLLDRLDYAKVQLRYLPASGPAAAGRGAAESPDREAVNRNERR
                     WEALGRGEVAAARSADGSSSAEVTPLLATKWNQDTPFWNYTPRVGGEPTPAGCSAVAM
                     AQVMNYWKHPAQGQGSHSYTWNGQTLSADFNHPYHWSLMRDTYYAGTYTAAQADAVAR
                     LCSDVGISIDMQYAVDGSGSYINDNDALTAFFKYSPDAHETDRAEAGSWEAWFEVIRR
                     EVEIHQPVIMAIYNRAEESGHAVVADGYRTSPSNQLHINMGWGGFADNYYSVDHIYHF
                     GDAEWDYAVIGIHPMRIKLTLKATEGGTTVPSPGTYVYPYGTTQIVRATAVPETHYAF
                     TGWTGDVSGTQNPVDIVVDMEKTATANFLRNIYAPLSASGQKILNRSFSQAEYINLIT
                     FEPNPDNVDIKFHRLYEVEGTKRTEIALLETAVEFQHRGVLKDKTYTYHIVAVNSASR
                     EGAPAVLVVQ"
     gene            17431..19065
                     /locus_tag="PKV64_05080"
     CDS             17431..19065
                     /locus_tag="PKV64_05080"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPN16490.1"
                     /translation="MKPRSVVCLLTMVFTLASAAYPCTIIVAGKKATVDGSVLNSHTD
                     TCNNSRLMVVPGRTFPKGTLAPLHWGIQDVALPLGDFGEVIGRIPQAERTYSYIHSGY
                     PHINEHQLAIGESTTSQREELRVEREGAKQIMTVEQAMALALQRCKRAREAVAMIGEW
                     METYGFLPSCGPESEDLCIADPDEAWIMEMFSVGPGWEPGKGIPGVIWAAQRVPDDEA
                     AIVPNWSIIKEIDLDKPDWFMASSNYQQFAIDRGWYDPAAGKPFVWQEIYAPIPREWA
                     TGRFWLFFQTIAPRLRAWPERQITGPFSGIDPYHQYVEPLSLYPFSAKPEKPLGVEDL
                     MKLQRSVNEGTIYDMTADPDWLIPDGQGGMKLSPLTTPFPTRPMRELLKISSRRNVAR
                     GNYGMIAQLRGWLPDPIGGVYWVYLDNQYTGIYVPIHAGVERINPLYSTYDPDRFSED
                     SARWVYDFAENLLYLKWQEAIRDLRAVRDPLEREFLEKLPEVDKKALELMKKNPGQAR
                     TFLTESAWTNMDRAVKAYRELRSTLISKYTNNKQGS"
     gene            complement(19140..21167)
                     /locus_tag="PKV64_05085"
     CDS             complement(19140..21167)
                     /locus_tag="PKV64_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HPN16491.1"
                     /translation="MLPPLEKALFALAVLATAVLFLRPMILRIRIIAAGHSENRSDRP
                     VRRLGAALGKILLQRCTLKNERPFTGLMHVFIFYSALTFDTLTVNHTLEGFFSGFSLL
                     GHGRFRMFFALLTDVMAVLVVAGTLYFIVRRFLLRPKAYRTTPLDSAVIYAFLILVTA
                     SFLYYEAFAIAARGGARGYAFLGNLLADAIRGAGVLPAAVAAHEHAGWWLHILLVFGF
                     IAYVPHSKYLHMFAGSFGMLVRQPGSGRIIPTLDLENSETFGIEKAADFSWKDNLDAF
                     ACMECGRCQDACPASRTDKALSPKMILVDMEKNLLAERKAVLARDRDAQKPLVPDVHA
                     EDEIWACTTCGACMHVCPVEIEHIRKIVGMRQSRVLMESKFPAPLNALFRNLETNANP
                     WGVGFAERGRWAEGLNLRTIADHPDAEYLFWVGCAGSFEEEGRRTARAFARLLTAAGV
                     DFAILGTEEKCCGDAARRLGHEYLFQSLAAETIETFARHKVRKILTTCPHGYNAFRNE
                     YPRLASGLASLSPEARAHFPRIEIRHHSEFLLELIASGRLKPAPGTGTRIAYHDPCYL
                     ARHNGLADAPRAVLRAAAGAPPVELADNREHSLCCGAGGGLMWTEETAGTRINHLRAG
                     QILEADVAAVATSCPFCLTMLRDALVDKDRADVAVRDLAQILAEAAGLAAD"
     gene            complement(21177..23948)
                     /locus_tag="PKV64_05090"
     CDS             complement(21177..23948)
                     /locus_tag="PKV64_05090"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPN16492.1"
                     /translation="MALPKTRTQIRILFCSAACLCLFFFLASPPPAEAGAETLVWGRV
                     LDAGGQPIPGTVVSVSGEDLEEEASVSADAAGWYALVGLRPGAYAIRAAAPLYAAVVR
                     PRVVCEPDGRVFAAFTLEKTGAGTGSAAVREDLFPNRRVYIPAVQIESLPTGRSVDSL
                     IENQASTATADRIDVGGMWEAVPTLFGSRGAASWTQNVFLWNGQDVTEPFEGGTPLVV
                     PDLFALESFFLTDSAVPIQALTPGSTVDLTPFRGTSSFHGAAQGFYLDKSFASSNITP
                     ALREEGLTESDTFNRLMDVSLRLSGPLFGGRWTYFTSWAVDTAARDLADYEPLDESRL
                     VSGLVHLVRDGDRRRLRLFWTGQAASHSSAGAAGGVPPETTVRKNEGRHLVQILSESR
                     PGGRSSSRYGLSFCLAGWNDDLQNGASGVQRIEKFLNIPEGTAAQTGDEKRVRASVFF
                     DGRSFFRNVVRTHHLFEYGVRLHYAGSESAASVPGDLRLVFYDGRPLEALVFDSPFRH
                     RQETAGLDAYVQDTINLAGGVSLSLGLHGAAVYGRGGGGSIRSLNFSPRFALRIPLSA
                     AGSSAVRLSVVRYSWTLPLSWLAWGDPAAPGRLAFRWNDADGDTGWSEDERGPLLRRE
                     GPRYGGIDENLRLPYTDELTLAFIRGMGRGWMMSLAGFLRETRNLVETVNTGVPGAAY
                     DPVSFFDIGDDRIPGTIDDLEFTVYDQRPETFGRDFHLLSNPGHGSRKSTYAGLDFIL
                     FKRPTDESLFFLAMTATRAFQTNGPGNTAWENDEGMIGPLYDNPNAGINAQGRPRFDR
                     AYTIRLGISHELPFKTRAGLVVKYYDGQPFTRMIIVEGLNQGPVMIQAHGRGVARYEF
                     NLTADFRLEKIIPFPFGSIRLMFDVFNLFNMHQATSENPWTGPEFPLRYATGIQSPRI
                     FRLGFHFAF"
     gene            24106..>25012
                     /locus_tag="PKV64_05095"
     CDS             24106..>25012
                     /locus_tag="PKV64_05095"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPN16493.1"
                     /translation="MTLTKDIAVIGGGPGGYVAALRAAQLGKSVVLFEKEWIGGTCMN
                     VGCIPTKHLLHQTSLLRALRTAKTFDGPAGEVTLNWTRVLEEKRKVIDRLVRGVEFLL
                     KSHKVEVVKGTAVLGGGRRIVVRNGEGEEQAYEAEAVILAHGSRPAELPFLKFNGTDI
                     LSSTEMLDLAAPPKSLAVIGAGAIGLEMGTIYSRLGTDVTVLEILPSALPGCDREMGQ
                     RFELMLKKQGLKVLTQSRIESCERGPDGLVVLKGTSLKTSAPFACAAEKVLLAAGRRP
                     NSGGLSEGASPVALDKRGFVQVNERL"
BASE COUNT         4282 a         7745 c         8440 g         4445 t
ORIGIN      
        1 aggagagccg gcccttgtgg acggcgatcc cggcccggat gacgcggatt tcagcgctgc
       61 gggcgatctt gccctccagg acatagcagc cggcgatcgt gccgaccttg gggatccgga
      121 agatcttccg gacctgggcc cggcccagga acgtttcctt gatcaccggc tcgagaagcc
      181 cggtcagggc cttcttgatg tcgtcggtga gctggtagat gatcttgtag gtccggatct
      241 cgacgttctc cttgccggcc gtctccagga tcttcgggga cggcttgatg ttgtatccga
      301 tgatgatggc ctgggtcgtc tgggctagga caatgtccga ttcgttgatc gagccggtgg
      361 ccgcttggat gatgcggatc ttgaccttat cggttccgag ggaagggaga agcccggtca
      421 ggacctcgat cgagccctgg acgtcggccc ggatgatcaa gggaagctcc ttggccgcgc
      481 cggtttcaac cttgcggaaa aggtcgtcga gcgaggcgac ggcctcttgt ctttgcggtt
      541 cggccttggc ggccgcttcc tttctttgtc cggcgatgcg ccgggccgtc tccaggtccg
      601 gcaggacctg gaggaaatcg ccggcccggg ggacatcgga gtatcccagg atctccacgg
      661 gcgaggacgg tccggccgcg ggcatcgtcc gcccgaggtc gtcggtcatg gctcggacct
      721 tgccgaacgt cgttccggaa agaaaggtgt ccccgggccg gagcgtcccg ttctggatga
      781 taaccgtggc catcgggcct ttccggggat cgagccgggc ttcgaggacg acgccctggg
      841 ccggggcgga gggattggct ttcaagtcga gaacgtcggc catcaggagg atcatatcga
      901 gaagctccgg aatgtttttc cgttccttgg ccgagatctc gacgctgatg acatcgccgc
      961 cccattcctc cacgagcaat ccgtcttttt gcagctcccg cttgatccgg tccggattgg
     1021 cttcgggctt gtcgatcttg ttgatggcca cgagcagggg gacgccggcg gccttggcgt
     1081 gattgatcgc ctcccgggtc tggggcatga gcccttcctc ggcggccacg accaggatga
     1141 cgatatccgt caccttggct ccccgggccc gcagctgggt gaacgcctca tgacccggcg
     1201 tgtcgatgaa cgtgatcgcc tttttattga cggtcacctg ataggcgccg atgtgctggg
     1261 tgattccgcc cgattcccgg tcgaccaggt tggatttgcg gatcgcgtcc agcagcgtgg
     1321 tcttgccatg gtcgacgtgt cccatgatcg tgacgacggg cggacgatgg gccaacagct
     1381 ccggacgggc cgccgcttcc aggcggcagg attcctccac cgggacgatg tcgagaacga
     1441 tgttgagatc cttggccagg gaagcggcca gggaggggct gatgatatcg gtgatcgcca
     1501 ggttgtaccc cttgacggcg agcttgtcgg ccagatcccg gggccggatt ttgaaccggt
     1561 cggccaggtc cttgatcgtg gctccctccc gcaggcgggc ttggggccgg ggccgggggg
     1621 cggccggctt gggggcggga gacgcggcgg aaacgggggc cttcgccgga ggcgcggggg
     1681 cggccgcctt gggcccggga gatggagcgg aaacgggagc cttcgccgga gccgcggggg
     1741 cggccggctt ggccgcggcg gcgcttttct tcggggnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnttcg ccggagccgc gggggcggcc ggcttggccg cggcggcgct
     1921 tttcttcggg gccgttccgg ccgccggggg cttggaagcg gacggaggag gagcgggcgc
     1981 cgccggcttg accgcgggag gaggggggga ggcgggaggc gtcgcggcgg ccttggtttt
     2041 cggtttcgtt gttttctttt cgctcacggg ggattccttt gaaaaaatta acccagctcc
     2101 ttgatggcga tcttccaacc ggtcagcttg gaggcgagct tgacgttgat gcctttcttc
     2161 ccgatggcca gggaaagctg gtcccgggag acgagcgctt ccagcgattt atcggccgtc
     2221 gagaccggga tgacgcgctc gatccgggcc gggttcaggg cggacttggc gtaggctccc
     2281 ggattgggcg accagaggac gatgtcgatt ttttcgccgc cgagttcctt gctgatcgcc
     2341 aggatccggc ttcccttggg gccgatgcag gcgccgatcg ggtcgacatc gcgctcccgg
     2401 ctgatcacgg cgactttcga ccgctcgccg ggctggcgga cgatgtcctt gatctcgatg
     2461 atgccggacg agatttcggg gatttccgtt tgaaggaggc gcccgaggaa ctcgttgctg
     2521 gaacgggaga cgatgatctg cgtctcctgg cctttctgtc cccgccgcac ctggacgatg
     2581 accgcccgga tctgggcgcc tcggtcgtag gtctcgccgg ggatcatttc ccaggggggg
     2641 aggacgacgt ccaggttatt gacgtccagg atcagggcgc cgttttcgat ccgccggtaa
     2701 gtcccggtga cgatttcccc gatccgatcg gcgtaggcgt cgtatatttt ttccgattcc
     2761 gcgtcgcgga ctttctggac caggacctgc ttggccgctt gggccgcgat ccggccgagg
     2821 gtgtcggacg gaaggtcgat ttcgatcgtc tcgcccacgg ccgcgtcggc tttcaaccgc
     2881 tgggcctcgg gcaggctgat ctcccggccg gcgtcggccg gcgcctcgac gacggttttc
     2941 aggcaataga cccgcagatc gcctttttcg ggcttgaagg tgatttgaac gtcctcattc
     3001 cgcgtgtaaa tccggccggc cgcgacccga agggattcct cgatcgccgc gacgatgact
     3061 ttcggctcca caccgcgctc cttgctgagc tgcagaatgg tattccagac gttgattttc
     3121 aagacatcct cttcattttg ggggatccgg tcggaatgaa acattatatc ggaaagagac
     3181 ggggagttca aattcgccgg tttttgcgat ccgtccggcg gcggagggga ttttgacatg
     3241 acgggcgggt ttttatatat taagcgccac ctccggggag ggttcgccga aaccggagcc
     3301 cgattttaat tatttgaggg agaagacgat ggccgcacac ctgttctggc tcgcgcctct
     3361 cggatcgatc ctggcgctgt ttttcgcttg gtttttctat caccaaatga agaaagcccc
     3421 cgagggaagc gagcgcatgg ctcaaatcgc cctgtacgtc cgccaggggg ccatggccta
     3481 cctgaagcaa cagtacaagg tcgtgacgat tgtgtttgtt tttctggtca tcttcctggc
     3541 gatcctggcc ttcctcttca aggttcagaa tccttgggtt ccggtggcgt tcctgaccgg
     3601 cggattcttc tccggcctgg ccgggttctt cgggatgaaa acggccacct atgcggccgc
     3661 ccggaccacc caggcggccc gccggtccct cgacagcggt ttgcggatcg ccttccgcag
     3721 cggggcggtc atgggtctcg tggtcgtcgg gctggccctt ctcgacatct ccctctggtt
     3781 tcttatcctg aaagccatct atcccgtcaa cgacggcggg cacaatctga tcctgatcac
     3841 cacgaccatg atcacgttcg ggatgggcgc ctccacccag gcgctcttcg cccgcgtagg
     3901 cggcggcatc tacaccaagg ccgccgacgt cggggccgac ctcgtcggta aagtcgaggc
     3961 cgacatcccc gaggacgatc cgcggaaccc ggcggtcatc gccgacaatg tcggcgacaa
     4021 cgtcggcgac gtggccggca tgggcgcgga cctttatgaa tcctacgccg gctccatcct
     4081 ggccacggcc gccctgggcg cctcggcctt cctggggact cccgacctgc agctcaaggc
     4141 ggtcatcgcc cccatgatcg tggccgccgt cgggacgctc ctttcgatcc tgggcatttt
     4201 cctcgtccgg acgaaggaag gcgcttccca gaaacagctc ctgggggccc tcggccgcgg
     4261 cgtcaacggc agctcgatcg gcatcgtcat cgtctcgttc gcggtcatca ggctgctcgg
     4321 ccttcccctg ggcgtctggc tggccatgat cgtcggcctg gcctcgggga tcgtcatcgg
     4381 ccgggctacc gaatatttca cctcggcctc ctacaagccg acccggacgg tcgccgagaa
     4441 cgccaagacc ggtccggcca cggtcatcat cgccggcttg ggcgtcggga tgctgtctac
     4501 gacgatcccg gtcctggccg tcgtcgtcgg gacgatcctg gcgttcctct tcgcctcgaa
     4561 tttcgacctg aacaacgtca gtcacggcct gtacggcatc ggcatggccg ccgtcgggat
     4621 gctgtcgacc ctgggcatca ccctggcgac ggacgcgtac ggcccgatcg ccgacaacgc
     4681 cggcggcaat gcccagatga gcggcttgga ggaggaggtt cggaagcgga ccgacgccct
     4741 cgactcgctg ggcaacacca ccgcggccac gggcaaggga ttcgccatcg gctcggccgc
     4801 cctgacggcc ctggccctcc tggcctccta tgtcgaggag ctgaaggtcg gattgatccg
     4861 gatcgggcgg acgatcatcg aattcgccga cggaacgacg atcgatacgg cccgggccgg
     4921 attcatggat ttcatgcggt attacaacgt caacctaatg aatccgatcg tcctgatcgg
     4981 aatcttcctc ggggcgatga tggccttcat gttctgcggc ctgaccatga cggccgtcgg
     5041 ccgggccgcc tcccgaatgg tcgacgaggt ccggcggcag ttccgggaga ttgccgggat
     5101 catgaccggc caggccgagc ccgactacgc ccgatgcgtg tccatctcga ccaagggcgc
     5161 ccagcgggcg atgcttttac cctcgctgct ggccatcgcc ctgccggtcg tgaccggcct
     5221 tcttctgggc gtccccggag tgatgggcct cttgatcggc gggacttcca ccggcttcgt
     5281 cctggccatc ttcatggcca attccggcgg agcttgggac aatgccaaaa aattcatcga
     5341 ggaaggccat tacggcggca agggctcgac ggctcacaag gcggcggtca tcggcgacac
     5401 ggtcggcgat cccttcaagg acacgtccgg ccccagcctg aacatcctca tcaagctcat
     5461 gagcatggtc gcgatcgtca tggccggcgt gacggttgtg gttcaccttc tgtgatcggc
     5521 gttttttaag acgattcgac gcccgctccg gagacggggc gggcgttttt ttaaccgcgc
     5581 tccgctatcc ggcgacggcg atcccgcgca ggctgtaagg gcccgcgtcc gtgatttcga
     5641 cctcgacgaa ccggccgacg acgtcggcgt tcgaccggaa attgacgacc tggtttccct
     5701 cgttccggcc ggcgaacacg gccgggtcct ttttgctccg gccggtgcag agaacacgtt
     5761 cccgcccccc gatcagcgtt ttatgaaagg ccagctggat gtctttctga agggcttgaa
     5821 cctccatcag ccgccgtttc ttgatttcag ccgggacgtc gtccgtcaag cgggccgcgg
     5881 ccgtccgcgg ccgcggcgaa tagcagaagg agaaaatgcc ggcgaagcgg atttcggcca
     5941 gggccgagag ggtttccttg aattcggctt cggtttcgcc cggatagccg acgatgatgt
     6001 ccgcgctgag gcggatggag ggcatgagag cccgaagggc ggccgcggtt tccaggtagt
     6061 ctttccgggt gtatccccgc ttcatccgct cgaggaccga ggccgagccg gattgaagcg
     6121 gcaggtggag ctgccggcag atcttcggcg tggcggccat cgtccgggcc aaggcgggcg
     6181 ggaagctttt cgggtgcgag gtgaggaagc ggatccattc cggcccgggg acggcggccg
     6241 cccgcccgag gagggcggcg aaatccgtcc ccgactccgg gtcgcggtaa tggttgacgt
     6301 tctggccgag cagctggatt tcccggtacc cgcgggcggc cagctcccgg acttcttcca
     6361 aaaccgcctc caccggccgg tacttttccc gcccccgggt gaagggcacg acgcagtagg
     6421 cgcaaaaatt gtcgcagccc tccatgatcg tgacataccc gctgacggcg ctttcccgga
     6481 gaggggccgt tttcttctcc cgccagccgc gcgtccggga gacggcgacg acccggcggc
     6541 gggcgctttc cgcggccact tcggcgatcc ggtcatactg gtcgaatccg aggacgaaat
     6601 cgacgccgga tttcgggccg gccaggctct ccttccggag ttgggcgacg caaccggcga
     6661 cgccgacgag aagcgggcgt ttctttttca gcatccggag gcgtcccaaa aaggactgca
     6721 gcttttcctc cgacttggcc cggaccgagc aggtgttgac gacgacgaga tcggcttcgg
     6781 cgaccgaggc ggccggggaa gccccggcgg cccgaagaaa cccggccagc cgctcggagt
     6841 cgttctcgtt catctggcag ccgaaggtgt ggacggcgaa ccgcagtccg ccgagagggg
     6901 ggccgggggc ggtcatcgga ggaggtcttc gaggatcgcg gccgcgtccc ggacaaacgt
     6961 cgcgcagcgc gtttgaaaga gatttccgtc ccgagctttc ttttggcccg cggccgtggc
     7021 caagtcgcag ccgatgaggg ggggacacga gagatcgtcg ccgtggcggg cccggaaccg
     7081 ccgggcgaat tcggtcatga cggcgtaggt tttttccttg gccgccgagt cctcgacccg
     7141 gacgcgtccg tgcctgaggc cgatggccag gaacgcgccg gtgacggctc cgcacgtgaa
     7201 gccgaggtgg gccatccctc cgcccagcgc ctggctgagc ttgagggcgg tttccgtatc
     7261 gagcccggcg tcttcggcga agggggtgaa aacggcttgg gaacagctga agccggcctg
     7321 gaacaaccgg ccggcggcgt ctggtttgga catgattgat ttctccgcgc ctattgtacc
     7381 taaggccggc ggtttgagga aatccgttcc ccgaagcgct tccgcgagga gtcagggccc
     7441 ggcgtttcgc tccagcatgt cccgcgctcc ggccagagag gccggcgtct ggaggcttcc
     7501 ggacatgaga cgggcgattt cctcgatccg ctgatccggg ccgagcttcc ggatcgtggt
     7561 gaaagtccgg tctttctcgg tcttcttggt gatcgtgtaa tggtgggcgg cgaacgaggc
     7621 gatctgggga agatgagtga tgcacaagac ctggtggcgg gcggcgagcg ccttaagccg
     7681 ctgggcgacg aattcggccg tcttgccgcc gatcccggcg tcgatttcat cgaagatcag
     7741 ggtggaggcc gcgtcttttt cccgggcggc cgacttgagg gcgagcatga tccgcgacag
     7801 ctctcccccg gaagcgattt tccgcagcgg cttgggcggc tctcccggat tgggggagat
     7861 gaggaactcg acgtcgtcca agccggcttc ccggaccgag tcgggctggt ccggggaaaa
     7921 aggccggccg tcaaggcgga cttcgaagcg ggcgttcttc atcccgagca gggcgatctc
     7981 tttttcgatc agggaagaca gctcgcgggc ggccgtccgg cgggtcttgg acagcttttg
     8041 ggccgaggcg gcataaacgg ccgcggccgc gacgatgtcc gcgtcgaccc gaagcaggcg
     8101 gtccttgatt tgaaggaaat cctcgcgttc ggctttcagg cggtcgaggt agccctggat
     8161 ttcgccgatt tccgccccgt attttctctt gaactgttcg atcaagctca gccgttcctc
     8221 gatctcctcc agccgctcgg gggattcctc cccccggtcg cggaaacgga ggagcaggtc
     8281 ggaaagctcc cggacggtga tcccgaacga ggagagggaa tccttcatct cccggaaatc
     8341 cggatggaag gggctcagct cctggagcag gtgttcgagc ttgacgagcg cggcggacag
     8401 cgaagcctcc ccgccgtagg ccaagtcgta ggaggcgtcg agaaggccgc ggattttttc
     8461 cgcgttccgg aggagaaggc gcttttcccg cagctcctct tcctcgccgg ggcggagccc
     8521 ggctttttcg atctcggcga tctggaactc caggaaatcg agccgctggc cgcgctcccg
     8581 ctcccgggcg gtccattcct ctttctgccg ggccaggccg cgcagctccc gggcggcccg
     8641 ggcggtttcc tcgcgcaggg ggatcgtccc ggcgaactga tccaggtagg cgagatggct
     8701 ttcgatctga agcaggaaga tatgatcgtt ctggccatac acgtcgatga ggccggccgt
     8761 cgtttccttg agccttttca agggaaccag gattccgtcc tggtaggcct tgcctccgcc
     8821 ggcggcgaga agccgctgga ggatgaggtc gccttcgccc tcctcgccgg ccggccggtc
     8881 gaagatcgcc tcgacggagg cttcggcggc tccggcccgg acaagatccg ccgatccttt
     8941 ttcaccgcag acgaggcgga tcccgtcgat gatgatcgat tttcccgccc ccgtctcgcc
     9001 ggtcaggacg gaaaatccgt tctcccaatc aatccggata tcctcgatcg tggccaggtt
     9061 gcggaggcgg aggtacttga tcattcggcg gaggactcag ggacggccgg cttcgagctt
     9121 cagggggacc agggccttgg ccatgttgat ccggccgcat ccgatggagt cgtcgatccc
     9181 cggaagcgtc gccgcgttga cgtcgtcggc gctgagacgg atgatttccc ggatctgggc
     9241 ggccgtcagc cagggtttca ggcttttcag gatggccgcg agcccggaga catggggggc
     9301 ggccatcgac gtcccgtcga accggctgta cggcaaagga ccggaaccga gaaaccacgt
     9361 cggcacggcc gtcaggatcg acacgcccgg ggcggccacg tccacctcgg gtccggcgtt
     9421 ggagccttcc cagacgtcgt cgttgtaatc cgtcgcggcc acggccagga cgtaatcatt
     9481 gtaggccgcc ggatactgga cggggccgcc gttgttgccg gccgcggcga cgaggacgca
     9541 gccctggtcg gaggcatagg cgaccgcgtc gaacagcacc tggctgacgt cgttgaatcc
     9601 gaggctgagg ttgatcacct gggcgccctg gccggccgcc cagacgatcc cgtcggcgac
     9661 ttgggagacc gttcccgttc cctggtcgtt caacactttc accggcagga tcatggcgtt
     9721 ccagccgacc ccggcgatgc cctcgccgtt gtcggtggag gcggcggcga tgccggcgac
     9781 cgccgtgccg tggtacaggt cgtcggacgc gtcgtaatcg tcgttgacga aatcacggcc
     9841 cgggccgacg atttttccga ccagatcggg atgggtcagc tcgacgccgg aatcgacgat
     9901 cgcgatcgtg accgcggcgt cgccccggga ttcctcccag ccctgggtgg ccttgatgtc
     9961 ggccccggtc tttccctggg ggctccccgg aacgtcgccg atcgattggc cgatgttgga
    10021 cagggcgtat tgatacttga acaggttgtc gttcggcgtg accgacgccc gtccgatgta
    10081 attcggctcg gcgtacagga cgcggggatc ggcggccaag gcggccgctt tctcctcgac
    10141 ggcggttcct tccggaagaa gggcccggta cacgtccagg gcggcgatcc ggccgatcgc
    10201 ccggactctt tgaacggcca tggcggcggc ccggtgagcc ggggggattt cgggcctgaa
    10261 cttgatcaac acttctccgg gcgcgtacgt cccgttcggg tcgaatccgg acgccggtcc
    10321 ggcggatccc ttgggacgga catccccgac gagcccggcg ctcatcggaa gcacccaggc
    10381 gatcaagacg cccgcggcga gcaatcggag aatggccggc cgttgggtca tgatgatcct
    10441 cccccttaaa acaggaattc catccgcgcc gcggcccccg ggctgagatt ccagccgccc
    10501 tcgagcttct taccggggat caggaagcct tcggccttca agcggaagga cgccgaaggt
    10561 tcgatgatca tccccaggga cgcgccgacg gatccgcggc cgttgatttt ctttttttcc
    10621 gaaccgctga gcgagccgat cgtctcttcc atggtgaacg acgaccagag gccgtcatag
    10681 aaaacggagg cggtgggcgt aaaggtctcg aatccccggt aagcgagggt ggggccgagg
    10741 cggacggccg tgcccgaggc ccggccggag agccgggccg gctgctgaag ggcctccgac
    10801 gtccattcgc ctttgagacc gagggaaaag agagcctggg cggcggcgcc gatttcccag
    10861 tctcccggcc ggaacagggg aacctccatc tccgccccga ggacgatcgc gccgcgcgat
    10921 ccggcctcgt agtcgacgga aaagggcagg cgccggaaga tcaggccgtt ccagttttcc
    10981 gcgccgccgc cggccaggag ggagaacgac gccccgccgg acagctccag cccgaaacgg
    11041 atcctccccc ggatcgattt cagaaaagag gagccggcgt ccttgtccca aacgaccatc
    11101 cgggagccgg cctcggcttc gatccccgca tggatgcggt agaacggcgc cggttcctgg
    11161 gccgcggcgg ccgccggacc ggcgagaacg gccgccccca ggatcaagac ggcgatcgat
    11221 cgggcgcgca agggcatggt ccgctcctta ttgtttttgg ggggccggag ttttttccgc
    11281 ggctttcttt cttccggcat cctcgatttc cttcagcttt ttttgggcct tggaggcgta
    11341 tttgccgttg ccgtaatcgg tcacgacctt ggtgaaatac ggccgggctt cgtcgggttt
    11401 tccctgacgg tattgggagt cgcccaggat gaagtagact ttgtccattt ccgggtaaag
    11461 gggataattc atcacgacgt cggtcaggcg gctgatggcc ggacggtagg acctcttccg
    11521 gtaataatgc tccccgatga cgaaaatatg ctcggccagg cggttctcgc attcctggat
    11581 cttggcccgg gccgactccg cctcctcggt caggggaaac atggaaaccg ttttcttgaa
    11641 ttccgccagg gcttcctcgg ttttcgtctg gtcgcggccg ggtttcagca ttttgttgta
    11701 ggaacaaagg ccgatccgat actgggccag gggggccgag gggctggtcg ggtagagacc
    11761 gagaaagctg cggtactggg cggccgccag gatcaggctt ccctcgtcct ttttctgaaa
    11821 gtacgtgtcg gcgatggcca gcatcgcccg ctgggcgtag aagctctggg ggaacgattc
    11881 catgacctgc cggaacagga tcaaggcctt gtcgggattt tttttcatca gctgttcgcc
    11941 ctgcttgaac aaggcttcgt cggaaccggc gatgtccggg gagatcaccg cctgttgttt
    12001 ccggcacccg ggcgtcagag cgagcgcggc cgccagaacc aggacggcgg cggctccctt
    12061 gtgttttttc atgagaggct tccttgagtc gtcgcgggac ggctccgctt gtcataaaga
    12121 tcgagaacgt aggcccattc gcgctcgagg ccgtcgcgga gcgccgtccg cggccggtag
    12181 cccaggtccc gccgggcctt ggcgatcgcc gcgaacgtgt ggggcgcgtc tcctttttgc
    12241 tgttccatcc ggcgcgtttt gaccgggcgc ccggtgattt cccggaacac ggggaagagg
    12301 tcggacagct tttcccggtg cccgccgccg atgttgtata tctctccggc cgtcccgttc
    12361 cgctcggcgg agacgagggc cgcgacgatg tcgtccacgt aggtgaaatc ccgggtctgg
    12421 ctgccgtccc cgaacacggg gatttcccgc ccttccagga tcgccttgaa aaacttgtga
    12481 aaggccatgt ccggccgctg gccggggccg tagacggtga agaagcggag ggcgaccacc
    12541 ggcaggccga aattgcggtg atagagaacg ccgagctgtt cggccgaaag cttggtcacg
    12601 ccgtaaggcg aaatgggccg aagcgggctc gtttccttca tgggcaaatc gggcgtcaag
    12661 ccgtagaccg acgaggacga ggcgtagacg attttccgcg gcggccgttc cttggccgcc
    12721 tcgaggagcc gctgggtggc ggcgatgttg tgatggaggt aggaggcgaa gctccgtccc
    12781 cagctcgccc ggaccccggc ctgggcggcc aggtggtaaa ccgcctcgaa cgggcgcagc
    12841 gtccggttga gaggcagatc gttgagatcg gcctcgatga aggtgaagcg gcggtcggcc
    12901 tgcaggggac ggagattccg ccgcttgatc caccggggat agtaattcgt caggcaatcc
    12961 aggccgcaga cgtcgcagcc ttcgtccagc aggcgccggc agagacggga cccgatgaat
    13021 ccggcggctc ccgtgaccag gactttcatc gccgctcctt ccgggcggcg atgtccctca
    13081 tcgtccggat gaccccggcc gggtcggggc tgccgaagac ggcgtttccg gcgacgaaca
    13141 cctgggcgcc gtcctcgcag aggcgctcga agttctcgag gttgacgccg ccgtcgattt
    13201 cgacgagcgt ccgaaggcca cgttcctcga tccggcggcg gaggaggcgg atccgctcgt
    13261 gggaagcctc gatgaacggc tggctgccgc ggcccgggtt gacgctcatc aacaggacga
    13321 aatcggcgtc gggaagaatc gcctccaggg cggtcaaggg cgtggccggg ttgagaacga
    13381 cgccggcctt gcggccggcc cggcggatgg ccgaaacgtc gcggtggaga tggggggcgg
    13441 cctcgacgtg gatcgagatc cagtcggccc cggcctcggc gaagaggggg atcatcgccg
    13501 aggggttgtc gaccatgaga tggacatcca acgggagggc tgtgactttt ttcagggcgg
    13561 cgacatgggc cggcccgaac gtcagcatcg gaacgaaatg gccgtccatc acgtcgacgt
    13621 gaatcaggtc ggcgccggcc ttctcgacca tatgggcggc gtcggccagc cggctgaagt
    13681 cggcggaaag aagcgaaggg gcgatctgga tcatgtcagc ggctcacttc gaaatggatt
    13741 tggttgcgct tgaggatcag ggacccggcg accgggctct gctttaaaat caccccgggg
    13801 ccgatatccg gataccaggc gtagctgacg tcgtatttca ggaagcccag ggatcgcagc
    13861 tgccgcagga cggagtccgc gttccgggcg atgagatcgg gcatgatgta gcgggggtct
    13921 tcctggccgc ggctgacgag aaagtccacc gccgaagccc ggttgacgac agtcccggcc
    13981 ggggggtcct ggaagaggat gcggccggcc gcggcctgcg gggtatggat ctgggaaacg
    14041 cggccccggc gcaatccggc cgctttgagc agttgggcgg ccatctcgac gctgcggccc
    14101 tctagccggg ggacgacgat ctgttcgttc cccaggctca cgacgacgcc gaccgtccgg
    14161 cgcggcttga cccgggatcc cttggccgga tcctgggtca ggatccgccc tttttccaag
    14221 cgcgtgtcga accgggaatc gacggtcgcc aggctgattt tcttgggagc caggagcgcc
    14281 cgggcctcct ccacggtctt gcccgtgagt tcgggcaaat tgatcagctc accccggtaa
    14341 atgagctcgg aaaagatgtc ggccgaggcg aaaaaaagaa aaagatagag aaggacgaaa
    14401 aggaggatgc tgttcgcctt cgtcgaggag gccattcact ttctgtcccg gagagtcggg
    14461 aaaaaatgta tcataaagca ttcaccggtg caaaccgccg cgccgttgcg ctataatgcc
    14521 ctgaagaaag cgaggtccgg aatgaatccc tctgattttc gcagcgatac cgtcacccgg
    14581 ccgacccccg aaatgagaaa agcgatggcc gaggcggagg ttggcgacga cgttatcgga
    14641 ttcgatccga cggtccagaa gctggaagcc atggccgccg aaaccatggg caaggaggcc
    14701 gggctgttct gcccctccgg gacgatgggc aattcgatcg ccgtcaaggc ctggacccaa
    14761 gaactccagg aagtcatcgt cgaatcccgg tcgcacattt tcaacctcga atccacccac
    14821 ctgacgttca tctcccgggt gacgccgcgt ccgcttccct cgcggcgggg ggccatggat
    14881 ccggccgagg tcgaacattt cgtcaagaag ccgaacgtcc acacgcccca gacgacgctc
    14941 atctgtctgg aaaacactca taacaattgg ggcggggcgg tcgtccccct ggaaaatttc
    15001 aaggcgatcc gggaggtcgc ggtccgtcac ggcctgcggg ttcacctgga cggcgcccgg
    15061 attttcaacg cctcgaccgc ctcgggcgtc ccggtccgcg gctatgcgga cgaagtcgac
    15121 tcggtgatgt tctgcctgtc caaggggctt tcggccccga tcggctccat gcttgtcggg
    15181 ccgaaggact tcatcgaata cggccggcgg atccggaagg ccttgggcgg aggaatgcgc
    15241 caggtcggcg tcatcgccgc cgccggtctc ctcgccctga cccggatgac cggccgcctg
    15301 gccgaggatc accgccgggc ccgccggctg gcggaagccc tctcggccct gcccggaatc
    15361 gtcctcaacc cggccgaagt ggagacgaac atcatcattt tcaagttcga gcatccggag
    15421 cttacggtcc ccggcttgct ttcggagctg gaaaagagag gcatccgggc tctcgccgcg
    15481 ccgggcggcg tcggcatccg gatggtcacc cacaaggacg tcgacgacgc ggacgtggac
    15541 gcggcgatcg gcgcgttccg ggacattctc ggttgacggg cggtctcgga ggacaattca
    15601 tccgtgagga gtttatgaat atgacgaagc ccaagtccgc tcggccggcg gccctgatcg
    15661 ccgccgtctg catcctgttg gccgcggccg ccgcggccga ggcccgaatc gtgggtccgg
    15721 ccgaggcccg gacagcggcc gcccgccttc tggaattcga aaacgcccgg cccgatctcc
    15781 gcctgacgcc gggcgtgttc gccctcggcc gcgtcgagcc cctgctccgc cgcggccgtc
    15841 cggtcgccta cctggcccgg ctcgagccgc ggggattcat gatcctgtcg gacatcaccg
    15901 agggatcgcc ccaggtgttc gtctcctacg agggagatcc ggaccggatg gcccggattc
    15961 cctacttggc ccgccttctc gaccgcctcg actatgccaa ggtccagctt cgttatctcc
    16021 cggcgtccgg gccggcggcc gcaggccgcg gggccgcgga atcaccggac cgggaagcgg
    16081 tgaatcggaa cgagcggcgc tgggaagccc tgggacgcgg cgaggttgcc gccgcccgtt
    16141 ccgccgacgg atcatcgtcg gcggaggtca ccccccttct cgcaaccaag tggaaccagg
    16201 acacgccttt ttggaactat acgccccggg tgggcggcga gccgaccccg gcggggtgct
    16261 cggccgtggc catggcccag gtcatgaatt attggaaaca ccccgcccag ggccagggga
    16321 gccattccta tacatggaac ggccagaccc tcagcgcgga tttcaaccat ccttaccatt
    16381 ggagcttgat gcgcgacacc tactacgccg gcacttacac ggccgcccaa gccgacgccg
    16441 tggcccggct ctgttcggac gtgggcatct ccatcgacat gcaatatgcg gtcgacggtt
    16501 cgggatctta catcaacgac aacgacgccc tgacggcgtt cttcaagtac agcccggacg
    16561 cccatgaaac cgaccgggcg gaagccggaa gctgggaagc ctggttcgaa gtcatccggc
    16621 gggaggtgga gattcaccag ccggtgatca tggccatcta taaccgcgcc gaggagtccg
    16681 gccacgccgt ggtggccgac gggtaccgga cctcgccgtc caaccagctc cacatcaaca
    16741 tgggctgggg aggattcgcc gacaactatt attccgtgga ccacatttac catttcggcg
    16801 acgccgagtg ggactacgcc gtgatcggaa tccatccgat gcggatcaaa ctgacgctga
    16861 aggcgacgga gggaggcaca accgtcccgt cacccgggac gtacgtgtat ccctacggca
    16921 cgacccagat cgtccgggcg accgcggtcc cggagacgca ttatgccttc accggctgga
    16981 cgggcgacgt ctcggggacg cagaatcccg tcgacatcgt ggtcgacatg gaaaagaccg
    17041 ccacggccaa cttcctccgg aatatttacg cccccctgag cgcctccggg caaaagatcc
    17101 tcaaccgttc cttttcccag gccgagtaca tcaacctgat caccttcgag cccaatccgg
    17161 ataacgtcga catcaagttt caccggctct acgaagtgga ggggacgaag aggacggaga
    17221 tcgccctgct cgagacggcc gtcgagtttc aacaccgcgg ggtgctcaag gacaagacct
    17281 acacgtacca tatcgtcgcg gtgaacagcg cctcccggga aggcgcgccc gccgtcctgg
    17341 tcgttcagta gatccatccg ttttttcccg tgaccggcgc ggcctgagcc cgaaagaatg
    17401 aatccgttcc gatgtgagag gagaaacatg atgaaaccga gaagcgttgt ctgcctgctg
    17461 acgatggttt tcaccctggc gtcggccgcc tatccctgca cgatcatcgt ggccgggaag
    17521 aaggcgacgg tcgatggatc ggtgctcaat tcccacaccg acacctgcaa taattcccgc
    17581 ctcatggtgg tgcccgggcg gacgttcccg aaaggaacgc tggcgcctct tcattggggg
    17641 attcaggatg tcgcgcttcc cctgggcgat ttcggcgagg tgatcggccg catcccccag
    17701 gcggagcgga cctattccta tatccactcc ggctaccccc acatcaacga acaccaattg
    17761 gccatcggcg aaagcaccac cagccagagg gaggagctcc gggtcgagcg cgagggggcc
    17821 aagcagatca tgacggtcga gcaagccatg gccttggccc ttcagcgctg caagcgggcc
    17881 cgggaggccg tggccatgat cggggagtgg atggagacct acggcttcct cccttcctgc
    17941 gggcccgagt cggaggacct ctgcatcgcc gatcccgacg aggcttggat catggagatg
    18001 ttcagcgtcg gcccgggctg ggaaccgggg aaggggattc ccggagtgat ctgggccgcc
    18061 cagcgcgtgc cggacgacga ggccgccatc gtccccaatt ggagcatcat caaggagatc
    18121 gaccttgaca agccggactg gttcatggct tcgagcaact atcagcagtt cgcgatcgac
    18181 cgcggctggt acgatccggc ggccggcaag ccgtttgttt ggcaggagat ctacgccccc
    18241 attccccggg agtgggccac cgggcggttt tggctgtttt tccagacgat cgcgccccgg
    18301 cttcgcgctt ggccggaacg gcagatcacg ggccccttca gcgggatcga tccctaccac
    18361 cagtacgtcg aacccctttc gctctatccc ttctcggcca agcccgaaaa gccgctcggc
    18421 gtcgaagatc tcatgaaact ccagcgctcg gtcaacgagg ggaccattta tgacatgacc
    18481 gccgatcccg actggctgat ccccgacggc caggggggga tgaagctgag ccccctgacc
    18541 acgccgtttc ccacccgccc catgcgggag ctgctgaaaa tttcctcgcg tcgcaacgtc
    18601 gcccgcggca attacggaat gatcgcccag ctccggggct ggcttcccga tcccatcgga
    18661 ggggtctact gggtctatct ggacaatcaa tacaccggca tctacgtccc gatccacgcc
    18721 ggcgtggagc ggatcaatcc gctctacagc acctacgatc cggaccggtt cagcgaagac
    18781 tcggcccgct gggtgtatga cttcgccgag aatctcctct atctgaaatg gcaggaggcg
    18841 atccgcgacc tgcgggccgt ccgggatccg ctggagagag agttcctgga aaaattgccc
    18901 gaggtcgaca agaaggcctt ggagctgatg aagaagaacc ccggccaagc ccggacgttc
    18961 ctgaccgaat ccgcctggac gaacatggac cgggccgtca aggcctatcg ggaactgcgc
    19021 tcgaccctga tttcaaagta tacgaacaac aagcagggat cctaagcgtc cccggccctc
    19081 ttgtcgcggc ggccggcgct tcccgggacg tcggacgtgc cccccggccg cccggcggat
    19141 caatccgcgg ccaggccggc ggcttcggcc aggatttgag ccaggtcgcg gacggccacg
    19201 tcagcccggt ccttgtcgac gagggcgtcg cggagcatcg tcaggcagaa cggacaggac
    19261 gtggccaccg cggccacatc cgcctccagg atctggccgg cccggagatg attaatccgg
    19321 gttccggccg tttcctccgt ccacatcaac ccgcctcccg cgccgcagca gaggctgtgt
    19381 tcgcggttgt cggcgagctc gaccggggga gccccggccg cggcccggag gacggcccgc
    19441 ggggcgtcgg ccaggccgtt gtggcgggcc aggtagcagg gatcatggta ggcgatcctt
    19501 gtcccggtcc ccggggccgg tttcaaccgg ccggaagcga tcagctcgag caggaattcg
    19561 gaatggtggc ggatctcgat tcgggggaaa tgggcccggg cttcggggct caaggaggcg
    19621 aggcccgagg ccagccgcgg gtactcgttc cggaaggcgt tgtagccgtg ggggcaggtc
    19681 gtcaggatct tccggacctt gtgccgggcg aacgtctcga tcgtctccgc ggccaaggat
    19741 tggaagagat attcatggcc caggcgccgg gccgcgtcgc cgcagcactt ctcctcggtc
    19801 ccgaggatcg cgaagtcgac gccggccgcg gtgagaagcc gggcgaaagc cctggcggtc
    19861 cgccgtccct cctcctcgaa tgagccggcg cagcccaccc agaagaggta ttccgcgtcc
    19921 gggtgatcgg cgatcgtccg gagattcaag ccctcggccc agcggccgcg ctcggcgaac
    19981 ccgactcccc agggattggc gttcgtctcg aggttgcgaa acagggcgtt gagcggggcg
    20041 gggaacttgc tttccatcag gacccggctt tggcgcatcc cgacgatttt gcggatgtgc
    20101 tcgatctcga ccgggcagac gtgcatgcag gcgccgcagg tcgtgcaggc ccagatttcg
    20161 tcctcggcgt ggacgtccgg gacgagaggc ttctgggcgt cccggtcgcg ggcgaggacg
    20221 gccttccgct cggccagaag gttcttctcc atgtcgacca ggatcatctt gggcgatagc
    20281 gccttgtccg tgcgggacgc cgggcaggcg tcctggcatc ggccgcactc catgcaggcg
    20341 aaggcgtcga gattgtcctt ccaggagaaa tcggccgctt tttcgatccc gaacgtctcg
    20401 gagttttcaa gatccaaggt cgggatgatc cggcccgagc cgggttgccg gaccagcatg
    20461 ccgaacgagc cggcgaacat gtggagatac ttggaatggg ggacataggc gatgaagccg
    20521 aagaccagca ggatatggag ccaccagccg gcgtgctcat gggccgcgac ggccgccgga
    20581 agcaccccgg ctccccggat cgcgtcggcc aggaggttgc cgaggaacgc gtacccccgg
    20641 gctccgccgc gggcggcgat ggcgaaggct tcataataaa ggaagctggc ggtgacgagg
    20701 atcaggaagg cgtagatcac ggccgaatcg agcggggtcg tccggtaggc cttcggccgg
    20761 aggagaaagc ggcggacgat gaaatagagc gttccggcca cgactaggac ggccatcacg
    20821 tcggtcagga gggcgaagaa catccggaaa cggccgtgac cgagaaggct gaagccggaa
    20881 aagaatccct cgagcgtatg gttgacggtc aaggtatcga aggtcagggc gctgtagaag
    20941 atgaagacat gcatcaggcc ggtgaacggc cgctcgtttt tcaaggtgca gcgctggagc
    21001 aggatcttgc cgagggcggc gcccagccgc cggaccgggc ggtcggaccg gttctccgaa
    21061 tgcccggccg cgatgatccg gatgcggagg atcatcggcc ggaggaacag aaccgccgtg
    21121 gccaggacgg ccagggcgaa cagggctttc tccaaagggg ggagcatgcg gcctcctcaa
    21181 aaagcgaagt ggaaacccag ccggaaaatc cggggggact ggatgccggt cgcgtaccgc
    21241 agggggaact cgggccccgt ccacgggttt tcggacgtgg cctggtgcat gttgaagaga
    21301 ttgaagacgt cgaacatcag ccggatcgat ccaaagggga acggaatgat tttttccagc
    21361 cggaaatcgg cggtcaggtt gaattcatac cgggccaccc cccggccgtg ggcttggatc
    21421 atgaccgggc cctggttcaa gccctcgacg atgatcatcc gggtgaacgg ctggccgtcg
    21481 taatacttga cgaccaggcc ggcccgcgtc ttgaagggaa gctcgtggct gatccccagg
    21541 cggattgtgt aggcccggtc gaagcgggga cgcccctggg cgttgatccc ggcgttcgga
    21601 ttgtcgtaga gcgggccgat catcccctcg tcgttttccc aggccgtgtt ccccgggccg
    21661 ttggtctgga acgcccgggt ggccgtcatc gccaggaaga acaaggattc gtcggtgggc
    21721 cgcttgaaaa gaatgaaatc aagcccggcg taggtggatt tgcgggaacc atggccggga
    21781 ttcgagagaa gatggaaatc ccggccgaac gtctccggcc gctgatcgta gacggtgaac
    21841 tccagatcgt cgatcgtgcc ggggatccgg tcgtcgccga tgtcgaaaaa ggaaacggga
    21901 tcataggccg cgcccggcac gccggtgttg acggtctcca cgaggttccg cgtttcccgg
    21961 agaaagccgg ccagcgacat catccagccc cggcccattc cccggatgaa ggcgagggtc
    22021 agctcatccg tatagggcag gcggaggttt tcgtcgatcc ccccgtagcg ggggccttcc
    22081 cggcggagaa gcggtccgcg ctcgtcctcg ctccagcccg tgtcgccgtc ggcgtcgttc
    22141 cagcggaagg ccagccggcc gggagcggcc ggatcccccc aggccagcca tgaaagagga
    22201 agggtccagg agtaccggac gaccgaaagc cggacggccg aggagccggc cgcggacaga
    22261 gggatgcgga gggcgaagcg cggggagaaa ttgaggctcc ggatcgagcc gcccccgccc
    22321 cggccgtaga cggcggctcc gtggaggccc agggacagcg agacgcctcc ggccaggttg
    22381 attgtgtcct ggacgtaggc gtcgaggccg gccgtctctt gccggtggcg gaacggcgaa
    22441 tcgaagacga gggcttccag cgggcggccg tcataaaaaa caagacgaag atcgccggga
    22501 acggaagccg cgctctcgga cccggcgtaa tgcagccgga cgccgtattc gaaaagatga
    22561 tgggtccgga cgacgttgcg gaaaaatgac cggccgtcga aaaacaccga ggcccgaacg
    22621 cgtttttcgt cccccgtttg ggcggccgtg ccctccggga tgttcaggaa tttctcgatt
    22681 cgttggacgc cggaggctcc gttttgaagg tcgtcgttcc atccggccag gcagaagctc
    22741 agcccgtagc gggaggagga tcgtccgccc ggccgggatt cggagaggat ctggacgaga
    22801 tggcggcctt cgttcttccg gacggtcgtc tccgggggaa cgccgccggc cgccccggcc
    22861 gacgaatggc ttgccgcttg gccggtccag aagagccgga ggcggcgacg gtcgccgtcc
    22921 cggacgagat gaaccaggcc ggaaaccagg cgcgattcgt cgagcggctc ataatcggcc
    22981 agatcgcggg cggccgtgtc gaccgcccag gacgtgaaat acgtccagcg cccgccgaaa
    23041 aggggacccg aaagccgcag ggagacgtcc atcaagcggt tgaaggtgtc gctttccgtc
    23101 aggccctctt cccggagggc gggggtgatg ttggacgagg cgaaagactt gtcgagataa
    23161 aatccctggg ccgcgccatg gaaggacgac gtccctctaa agggcgtgag gtccaccgtc
    23221 gagcccgggg tcagggcctg gatcgggacg gccgaatcgg tcaggaaaaa cgattccagg
    23281 gcgaacaggt cggggacgac gagcggagtt cccccctcga acggctcggt gacgtcctgt
    23341 ccgttccaga ggaagacgtt ctgtgtccag gaggcggcgc cccgggagcc gaacagcgtg
    23401 gggaccgcct cccacatgcc gccgacgtcg atccggtcgg cggtggcggt cgaggcctgg
    23461 ttttcgatca gcgagtcgac ggaacggccg gtcggcaggc tctcgatctg gaccgccgga
    23521 atgtacacgc gcctgttggg gaagaggtct tccctgacgg ccgcggatcc cgtcccggcc
    23581 cccgttttct ccaaggtgaa ggccgcgaag acccggccgt ccggctcgca gacgacgcgg
    23641 gggcggacga cggccgcgta aagaggggcg gccgcccgga tcgcgtaggc gccgggccgg
    23701 agcccgacga gggcgtacca gccggccgcg tcggcggaaa ccgaggcctc ctcctcgagg
    23761 tcctcgccgg agacggagac caccgtcccg gggatcggct gtccgccggc gtcgagcacc
    23821 cggccccaga caagcgtctc ggccccggct tcggccggag gaggggaggc caggaagaaa
    23881 aacaggcaga gacaggcggc ggaacaaaac aagattcgga tttgcgttcg tgttttcggc
    23941 aaagccatcg tattttcttc tgaaggcctc cggttgtcaa cgaaaaagga aagaggccgg
    24001 gagttcggaa tctccccgag cggcccggaa gattgaaaat atcggcgcgg tcgggtatct
    24061 tagcgtttcc gcggaaaaga gaaaacgacg acggaggaca agatcatgac tctcacaaaa
    24121 gacatcgcgg taatcggggg cgggccggga ggatacgtcg cggccctgcg ggcggcccag
    24181 ttggggaaat ccgtcgtcct gttcgagaag gaatggatcg gcgggacttg catgaacgtc
    24241 ggctgcattc cgaccaagca cctgctccac cagacgagcc ttctgcgcgc cctgcggacg
    24301 gccaagacgt tcgacggccc ggccggcgag gtgacgttga actggacccg ggtgctggaa
    24361 gagaaacgga aagtcatcga ccgcctcgtc cggggagtcg aatttctttt gaagagccat
    24421 aaagtcgagg tcgtcaaggg aacggcggtt ctgggcggcg gacgccggat cgtcgttcgg
    24481 aacggggagg gggaggagca agcctatgaa gccgaggcgg tcatcctggc tcatggaagc
    24541 cgtccggccg agctgccgtt tctgaagttc aacggaacgg acatcctgtc gagcactgaa
    24601 atgctcgacc tggcggcccc gcccaaatcc ctggctgtca tcggggccgg ggcgatcggc
    24661 ctggaaatgg ggacgatcta ctcgcgtctt gggaccgatg tgaccgtcct ggagatttta
    24721 ccctccgccc tgcccggctg cgaccgggaa atgggccagc ggttcgagct catgttgaaa
    24781 aagcagggcc tcaaggttct gacccagtcc cggatcgaaa gctgcgagcg cggtccggac
    24841 ggcctggtcg ttctcaaggg cacgagcctg aaaaccagcg ccccgttcgc ctgcgcggcc
    24901 gagaaggtcc ttctggccgc cggccggcgg ccgaattccg gcgggctctc ggaaggcgcc
    24961 tctccggtcg ccctcgacaa gaggggcttt gtccaagtca acgagcgttt gg
//
LOCUS       DAOOXH010000029        24539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_1857_length_24539_cov_7.967897, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000029 DAOOXH010000000
VERSION     DAOOXH010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 24539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24539
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1857_length_24539_cov_7.967897"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PKV64_05100"
     CDS             <1..792
                     /locus_tag="PKV64_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16494.1"
                     /translation="AAAAGKNGYFNLFHDFGGKGLMALSFTMAWTISPIALQRIRAAR
                     SASAARRGLGATAAALGGFYLLVVATGVLSLPLFPERIGGTSVRPLVADIAGGRTGFV
                     LGGLVFVAILAAILSTMDTAVNAGALVLNRDVIEQIHPRAKRRPVFWGRTATVLIAAF
                     AFLVALKLRNILKTIGLSSEILAEGFFIPGAAMMFLKAKYPAAGLFSVCGGGGFAVLS
                     FLESSGIVSLGLPAWPYSVPWGLAAGAAGFAAGFWVDRRRPDRLA"
     gene            836..1504
                     /locus_tag="PKV64_05105"
     CDS             836..1504
                     /locus_tag="PKV64_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPN16495.1"
                     /translation="MSAKTKFRLWLIQHPGKFLIWMWMKTCRWKIVGDGPYRALRKSG
                     RPVVLLIWHGKIFVVPFFFRKRGILALVSPSQDGEFVARLMDGWGYTLIRGSGSHPMK
                     TAWIEMVRELNEGGEVIIVPDGPKGPDRQFKPGAVKLAAETGAAMVPFSFSARRKKIL
                     RSWDRFEMFFPFTRIAAVYGEPIEIPRGLPPESREEERLRAERLMIEFDARVDRFFES
                     GAEG"
     gene            complement(1501..1884)
                     /locus_tag="PKV64_05110"
     CDS             complement(1501..1884)
                     /locus_tag="PKV64_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16496.1"
                     /translation="MNDHIKIIGILWIIFGALSLVAAGFLFLILVGVSFIPDIDPQAP
                     GILRFIGFFLGSFLALIGLPKIIGGYGLLKGYEWGRILTLVVSFLSLFNVPFGTALGI
                     YSIIILLNKETASRFQKSSPPDVRP"
     assembly_gap    2117..2216
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2217..2423
                     /locus_tag="PKV64_05115"
     CDS             <2217..2423
                     /locus_tag="PKV64_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HPN16497.1"
                     /translation="PFGGIQKTRVNPYDPALKFPGKEREPSNGMDYSGARHYPPDRYR
                     CLSPDPVLNRDAARSDPQPRPLQK"
     gene            2549..3334
                     /locus_tag="PKV64_05120"
     CDS             2549..3334
                     /locus_tag="PKV64_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16498.1"
                     /translation="MKKFLCLSVALLLALAVSAPAQTAVELIKAGDDARDQRDDAKAL
                     ESYLAALELEPENYEALWKASGSMVDVADLVNVRAKGGLEKQKKYYQDAAEYARKAIA
                     VNPNDTNGHFQLSAALGMYALGLGNKEKIAMSREIKAEIEKSIELDPNNDGAYHALGR
                     WHRTIAEIGGAKRLLGGILYGKMPKGTNEEALTYLIKAVEIKPDDIHHHLELGRTYVV
                     VEDYKEAAAEFRKCVDLPEASGKDAMYKQEAKAELRKIQNKLK"
     gene            complement(3335..3880)
                     /gene="amrA"
                     /locus_tag="PKV64_05125"
     CDS             complement(3335..3880)
                     /gene="amrA"
                     /locus_tag="PKV64_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPN16499.1"
                     /translation="MNGSLSIEKRRDLLHLARESIAARLTGEPTPALTTKDPLLAEKR
                     GAFVTLTRRGELRGCIGYPLPLKPLADAVVEMAAAAAFEDPRFEPVTAEEMDDLHIEI
                     SVLTLPVPAEKPEDVVVGRHGIIVSQGGRKGLLLPQVPVEYGWKRETFLSHGCLKAGL
                     PPDAWKKGARIEIFEAEVFDE"
     gene            complement(3877..4311)
                     /locus_tag="PKV64_05130"
     CDS             complement(3877..4311)
                     /locus_tag="PKV64_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16500.1"
                     /translation="MIKPSMDVFLTSDAARMLEAVRRLVPKKGGRGFLLGHRRGNRVY
                     VEAVLPSPSSAWPSLRSFYEMDAGLGRKIVGFFLIGAPATARAPLLRPFGTGKVLVEI
                     PLPGGKKRPPAGSLIDYHDRFLFKKIRVVVERPVGRPEGKRP"
     gene            complement(4329..5852)
                     /locus_tag="PKV64_05135"
     CDS             complement(4329..5852)
                     /locus_tag="PKV64_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HPN16501.1"
                     /translation="MAVVLDGKSLTLEKLVRIAREGEPVEVPPEAIAKIKACREMLEE
                     KIKAGEIMYGINTGIGEFSEVVLTDEQVGQFQKYLIYNHAAGIGDPAPVEHVRAAMAG
                     RINVHARGRSGCRPEITLTLAEMLNKGVTPVVCRKGSVGASGDLAPMSQVALLLMGEG
                     EAYYRGERLAGRVALERAGIPVPGLQARDGLAAINGSNLLTAISALTLYDMDRTLKQA
                     EIAATMTIEALIGNLKPYDVRLHEERGFPGAVRSARAILKCLQGSDLTTGKMKTKVQD
                     AYSMRSTPQVIGAAHDALVHARRQVEIELNATADNPIFLTENKLTLTGANFQGTPVAL
                     PMEMAGAALTMVCVLSERRLNRLTNPALSVGLPAFLTKGAGLFSGMMLSQYTADALVV
                     EQRILSAPACIQSIPAAADQEDFVSMGMNTALKNLQILDNAYGILGIELMAAAQGLDF
                     RPFTPGAGARAAKGVIRRHVARLDEDRPLHVDHNKMKALVQSGEILEAVEKAVGPLG"
     gene            complement(5836..6669)
                     /locus_tag="PKV64_05140"
     CDS             complement(5836..6669)
                     /locus_tag="PKV64_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN16502.1"
                     /translation="MAHALPWQLEVARRSLKKREKIDLIEQQVDVGPDDRCLDLGCAQ
                     GILSWFLKKKGGRWIHTDLDFENLLTSRDLLDRGFVQMGVPDLPFKSRSFDRVFCLDF
                     LEHVDDDLKTAGEIARVLVPGGKLLLVVPRTGPFILMQKIRPAVGLKLEFYGHKREGY
                     ALPALRMLLGRAGLRITRHAFYSRFFTEFVELVLNALYVNFFSAKPEARLRDGHIRPA
                     TPGEFAAQAKPFRIYKLVYPLLRLAAKLDTLLFFTRGYALMVWAEKTATPEEDPHGRR
                     P"
     gene            6774..7391
                     /locus_tag="PKV64_05145"
     CDS             6774..7391
                     /locus_tag="PKV64_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPN16503.1"
                     /translation="MKYQMIVVGPLETNCYLVYDEETRDCLIVDPGADADRIFPVIAE
                     ENLKPFAILNTHGHIDHIGANRDMKDKYDIPLYMHAGDKDRPSRLNQLELSLFIGAKD
                     SPPADKFVKDGDEIKVGRGALRVIHTPGHSPGSISLLDDGFLLSGDTLFFEGLGRTDL
                     PGGDQKQLEKSIRERLMTLPDEIEVLPGHGPLTTIGRERENLTFL"
     gene            7391..8293
                     /gene="xerD"
                     /locus_tag="PKV64_05150"
     CDS             7391..8293
                     /gene="xerD"
                     /locus_tag="PKV64_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HPN16504.1"
                     /translation="MSPAPPSVDIRAEFLRFLTVEKGLSPNTREAYGLDIDRFLRFLA
                     REKTSWDRARESDLAAFIRHESRAGLSARSLARRISVLKAFYRFLLLDGRLDKNPAIH
                     LTSPKTWLALPKALSEAEVKSLLGKPDPRDPRGLRDRAMLEVMYASGLRVSELVGLRT
                     ADVRLKDEFILCRGKGAKERIVPLGKTAAAAVVRYVEKARPALASKKGADELFLTRRG
                     EPFTRQGFWKMLKGHAREAGLQPKVHPHVLRHSFATHLLEHGADLRSVQMMLGHSQIT
                     TTQIYTHVSRERLRRIYDKFHPRA"
     gene            complement(8278..9000)
                     /locus_tag="PKV64_05155"
     CDS             complement(8278..9000)
                     /locus_tag="PKV64_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16505.1"
                     /translation="MRPAAKILKAGAAVCLLSAAVFLASGGAALPCPPQAWDIRMTIT
                     VQGEYALEGRDGASGRYGMRFVWRGGLEPDDEDYVLIHHGTKLEEWRVEESAAGPDGI
                     RLLTTRDYGVKPELGVAYILRKDGGLHLNFLIRGFEVPRSRPSEAFYLHLPASAENTD
                     RPEGLNYALFLQKGSNAIVLDDPAASRKTQEKSFHWTWFRRSGVLGRDPALFQMNRHE
                     ADVTVVVTPRADSGPRTGQARG"
     gene            9135..9836
                     /locus_tag="PKV64_05160"
     CDS             9135..9836
                     /locus_tag="PKV64_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPN16506.1"
                     /translation="MKPQKSRKSLSEKRSLRELDRRRRNREAILHAAEIVIGRKGLTA
                     SSMDDVAAEAGFSKATLYKYIQGKSELVMELLIHYLEDLNDRLAAILIKPVKPEAKLL
                     LLLREILRYQAEKANISRAFIMNPSHMQIFRAMAETGAMAASETEKDFFHRLQTARRA
                     MTGHLERFLREGIASGDFRPFPLEIMVRFLTAVILGYQHDQLFRESKPDIDKDIKNIH
                     EFILRGFKTESQADS"
     gene            9849..11189
                     /locus_tag="PKV64_05165"
     CDS             9849..11189
                     /locus_tag="PKV64_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPN16507.1"
                     /translation="MNRFRALIALILLAAVSGGPALAQEKMVLTLEDCLKLALAQNPF
                     YLAARAKEDQAEASLKEAVAGFFPTLNASGTDVLDKKVFTIEFPSFIPGGSPTRVKMD
                     FTRAYTFALSFSLPLYSGGRLVAGYKTANYNLEATREAIRQSREEMVLNVKKSFYGYL
                     LARSFVGVAEEGVALAEKHYTNVKNLAEVGMASKFDLLRSEVQLANLKPQLIRARNAL
                     ATAEIGLKMQLGLDLQKSVEFKGELAFRDVEADVDQNIVMALARRPEINQLNYQKLMA
                     AEMIKLNKGAGLPSLAVGGAYNYWSDHLNLKKENWESYYQINLVLNIPIFNGFGNAAK
                     VAQSKAAFRQLEYSQKGLIDSVKFEVQEAVLALRQARESLFSQEKNVEQAQEAVRIAD
                     LNYAEGLATNLDVSSAQVALSQAKTNHVQALYDYAVALAQIEKSMGTGSDRYEE"
     gene            11203..12309
                     /locus_tag="PKV64_05170"
     CDS             11203..12309
                     /locus_tag="PKV64_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPN16508.1"
                     /translation="MLKHRLPAVLLAAVVVFVPAACRKNETPAAAEAAAASLLQGTMP
                     VKVETVSRQRISEKMTFTGVVEAARKINITPETSGKVARIVVEEGQFVQKGQLLAELD
                     TASILLQLQQAEAAAAMAEANAKNSARNKERMDRLMAENAVSETQFEQVKLADEAAQA
                     QAAQARAAVALIRRALDQAVMTAPWNGVIASRNAEVGDILNPMMGGYGSSGSGVVTLV
                     DYGRVKIIVEVTQDDILRLARGQKAVVRSGSAEAAGMVSVVNLAADPLSKKFRVEILA
                     DNPGLVLRPGTFGSVTFEVNSHENALAVHQKAVLSDSYVFTAENGKAVKRPVTLGLKN
                     TTMIEVIDGLKEGDQVIVEGNFGLVEGTPVEIKR"
     gene            12316..15411
                     /locus_tag="PKV64_05175"
     CDS             12316..15411
                     /locus_tag="PKV64_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPN16509.1"
                     /translation="MQLPNFAVKRRVSTAMIALILVVVGVISFSRLGLDLFPDLEYPT
                     VSVVTTYRGASPEDIENLITKPLEGVVGSVNGVKKVTSITSEGTSAIQVEFEWGTNLD
                     FAAQDVRDRIGLYKGFLPEAASDPLVVKFNMAQIPVIFWGVVGDRSMSELKTIVEDEV
                     ARRFERLDGVAAATTYSMDTREILVDVNKDALASFGLAFDRVLGALMAGNMNMPAGYL
                     VERHSDFLVRTIGEFESLDDVRNTIVGSTAKGQPIYVRDVADVRDTLKETRFIGRIEG
                     EKAVYLMVSKRSGANTALVGKAVKAELENIKKTLPPDVKFLQIMDQSEMIQHVTKGTA
                     ATAWQGGLLAVALIFFFLMNWRPTLIIGVAIPLSIITTFIALYFAGYTLNLLTLGGLA
                     LGIGMLVDNAIVVIENMYRHIQEGKDALSAARLGASEVGMAITASTLTTIVVFLPMAF
                     ATGLTGMMTRGLALSVAFSLVASLFVALTIVPAMGSVLFQRKEGDAQAIRKTIGRDFT
                     GARNKYRRALEWALHHRKIVIFGTLGVFALSLFLIPVLGTEFMPSMDRGMLVMSVKTP
                     VGTSLEETNRIVTMIESVIRSRPEVQITSTQIGSSAEENAGDAGFGNPTGPHEAVFFV
                     GLKPTEERRLSDSQVVEEIRKRVPKFEGVKFESMDMSAMMMGGAAAPVEIKLFGKDLA
                     KLREIADGLVVRLKDVPGLRDVTHSLSQSKPEYHLKIDRDKAARFGLTVAQVESAVQT
                     ATLGQVATRYREEGEEYDVRVRFKDEFRETIAAIRDIPISTPMGTTISLDQVADVQPG
                     TGPIQITRDNQSRRISVTGNIVGRDLGGVVRDVQTRMKGLDEQLPAGYFVEIGGSYSQ
                     MTDMFKTLGWVFLLAVLMVYMVMASQFESLKHPFIIMFTIPLCFIGVVLGLLVFGHTL
                     NLAVGIGIILLAGVAVNNAIVMIDYTNQLRRDGMEIKEAILQGAVTRLRPVLLTALTT
                     ILGVFPMAVARGQGAEMRSPLGVTLLGGLTSTTFLTLFVIPVMYSLFERVRFKKTAAK
                     N"
     gene            complement(15517..16656)
                     /locus_tag="PKV64_05180"
     CDS             complement(15517..16656)
                     /locus_tag="PKV64_05180"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16510.1"
                     /translation="MPSPFRDRIVALSRFHGGPLLTTSFFLDTNLSKRTRREILLAAK
                     NMIAGGRADIASMDVSREIKSSLEKDLAALEAYFAGNLSSTVPGLAAYSCSGAGFWEA
                     LDLPEAPLDRLVVDRNPYIRPVTRILEDRRRFLVLTLDRREARWYDVYMSRIDPLSSL
                     SSDIPKKVKSGISGQEAKRIERHVEALVRAHLKRCAQTTFDILKKDGFAGLWLGCPDA
                     LYRDFEGMLHSFVRERIKGRLKVRPIDPLDVVLREAEDLERTLKKREEEDLLKMLIGE
                     IEKGTRARSGLRDCLAALNKGEVQTLVVTRNHFVPGKHCPRCRLLFLDELRCPACERK
                     TETRPDIVDEAIESALHRKCDVKYITPPSRLDHYGKMGALLRYKA"
     gene            16849..16935
                     /locus_tag="PKV64_05185"
     tRNA            16849..16935
                     /locus_tag="PKV64_05185"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16883..16885,aa:Leu,seq:cag)
     gene            17072..18241
                     /locus_tag="PKV64_05190"
     CDS             17072..18241
                     /locus_tag="PKV64_05190"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018919099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HPN16511.1"
                     /translation="MRKTAVSAMIGILGAGLVLGLAACRKEGPPAPPVAAETGKGGAN
                     MSVGKTVFGTLPDGSAVDLYTLADGKGLTVRLMTYGATLVSLEWPDRDGALADCVLGY
                     DTLDGYLKASPYFGATVGRYGNRIARGRFALDGNTYTLAANNGQNHLHGGVRGFDKIL
                     WKGEPVQDSSGVGVRFTTLSPDGDEGYPGNLDVSVTYVLTAGGELIITYVAKTDKPTV
                     INLTHHGYFNLTGGRRDVLGHELELAADRYTPVDAELIPTGLLAPVENTPMDFRTSVS
                     IGSRIAAVPGGYDHNYVLNSGGKTLASAARVFEPESGRVMEIRTVEPGLQFYSGNFLD
                     GSIAGKAGRVYGKHWGFCLETQHFPDSPNRPEFPSTVLRPGETYRTMTVFKFSAK"
     gene            18283..20361
                     /locus_tag="PKV64_05195"
     CDS             18283..20361
                     /locus_tag="PKV64_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008201203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HPN16512.1"
                     /translation="MRVPAPVRFLIVLLAFWAAGPAPAQDRLKTYPGYDRYAEIKDQI
                     KGAVKPGTVRAAWEDGGKAFTFTRGGKRLRFDIRRKTLTELGPAPEKAEEPVRRRPGG
                     LERGRQFTEALSPDGKTKAFYRDRNLWISDPDGKNEQAVTTNGDEKLRTKCGSASWVY
                     GEELNQITAMWWSPDSTKLAFYRFDESRVPDYVLQADQTRPYSRADVEAYPKAGRPNP
                     VVDVLVYEPAAKKTTVIDVRDGRPFENDVVGHYVYNVSWSVDSRELLFFRMNRLQNVL
                     EFAAADPVTGKCRTIIREEWPSGWVDIAPELAWLKDGRRFVWASARTGFKNYDLYDLS
                     GTRLAVLTRHPFEAAGIVRIDEKRNSLAYLARDGANEMMLQLHRVSLDGRNDVRLTDP
                     AYHHTVDFSPDGRHFIDTCQAHDVPPFSRLVDARGRIVADLVRSETAKFDELGLKKAE
                     LFTFAAADGTTPLRGLLFFPSRFDPSKKYPLLVSVYAGPETNGARETFAAPSALAELG
                     FLVASLDSRTAGGRGRRALDAVYGKLGIVEVDDQAAGVAALRARPYVDGSRVGIFGTS
                     YGGYVSAMALLRHPEVFAAACAMSAVTTWLQYDSIYTERFMGLPEENKAGYEAGEAMR
                     YVNDLKGRLMIYYGTADNNVHPTNAMLLIKALQDAGKSFDVQIGPDEGHTALNRERMM
                     EFFIEALVLK"
     gene            20405..20923
                     /locus_tag="PKV64_05200"
     CDS             20405..20923
                     /locus_tag="PKV64_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPN16513.1"
                     /translation="MNSSSAKTLGIVLVILAVLMAAGLLRALILIPLGIVEGFGHGLR
                     HWGGTWFWPWFGLTGFFGLFFLVLWIMVAVWVHGDAERRGMPGILWAVLVFFTHLLGL
                     IIYAIVRSSKSVKREGPPSAPPAEPEPAAPGNPSAESPPLIMPAKPAACSRCGKETRP
                     EWRICPFCGEKI"
     assembly_gap    21004..21103
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21245..22189
                     /locus_tag="PKV64_05205"
     CDS             21245..22189
                     /locus_tag="PKV64_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HPN16514.1"
                     /translation="MASKKNLVSNESIKGFIFDIKRFAVHDGPGIRTTIFFKGCPLHC
                     LWCHNPEGIDRRPELLLRSARCARCHACVKACPKNALSPGADGDPVTVDRSLCDRCGA
                     CVDACAYGALEIAGRQTTVADLVAEAERDRVFFESSGGGVTLSGGEPLAQPEFSLALL
                     EALKVRGLAAALDTSGAVEGDILDRAADLADLILYDLKTMDEAVHRRFVKTGNARILE
                     NLRRLNRRGRAVRVRIPLAAGVNDGEENIDASIRFLKTLASVRRVDLLKYHRGGEEKY
                     RNLGRAADFEIFEPPSDGRMEDIRRAFAEAGFNVSIGG"
     gene            22195..>24539
                     /locus_tag="PKV64_05210"
     CDS             22195..>24539
                     /locus_tag="PKV64_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HPN16515.1"
                     /translation="MTERVKKLRERSLAAVPSISGERAALLTSFYRSPEAGRCSPAVA
                     HALAFKRIMAEKTVCLEDGELIVGERGPAPKATPTYPEVNCHSVEDLRILDSRPKTFF
                     KCDPSVILLYEKEIIPFWRGRSIRDRMMEVLPPAWKDAYAAGIFTEFMEQRAPGHTVL
                     DDKIYKKGMEDFIAEIDAETARLDFLADPLAYDKREELRAMRISAEALIIQGRRHAEK
                     ARELAAREGNAARKKELLRIAEVCDRVPARAPRDFQEALQMYWFVHLGVITEYNTWDS
                     FNPGRLDQHLYPFYRKGLEEETLTEESARELLQCFWIKFNNQPAPPKVGVTAEESGTY
                     TDFALINLGGVKADGSDAVNELTYLLLDVIEEMRLLQPSSMVQISKKSPEKFLLRALK
                     IVKTGFGQPSLFNTDAIVQELVRQGKSVEDARNGGASGCVESGAFGKENYTLTGYFNI
                     PKVLEVALRDGFDPRTGKQLGPRTGDPAGFQTFDDLFSAFQAQLRHFIDVKIAGNAVI
                     ERLYATLLPAPFLSILIDDCLRRGRDYHAGGARYNTCYIQGVGLGSITDMLASLKHNV
                     FEKKIVTMKNLIAALDADFAGAEDLRQRLLNKTPKYGNDDDTADDHARRVFEAYFAAV
                     DGRPNMKGGSYRINLLPTTVHVYFGKVTGATPDGRKAGESLSEGISPVQGSDRKGPTA
                     VLKSAAKIDHIRTGGTLLNQKFTPQLLADEEGLRNLGHLVRAYFTMDAHHVQFNVVTA
                     ETLRDAQAHPEKYRDLIVRVAGYSDYFLDCSVDLQNEIIRRT"
BASE COUNT         4686 a         7517 c         7570 g         4566 t
ORIGIN      
        1 gcggcggcgg ccggaaaaaa cgggtatttc aaccttttcc atgatttcgg cgggaaaggc
       61 ttgatggccc tttcttttac catggcctgg acgatttcgc ccatcgcttt gcagcggatc
      121 cgggccgccc ggagcgcgtc cgcggcccgg cggggtcttg gggcgacggc cgcggccctc
      181 ggcggctttt acctgcttgt cgtcgcgacc ggcgttcttt cccttcccct ttttccggag
      241 aggataggcg ggacgtccgt ccggcctctt gtcgccgata tcgccggcgg ccggaccggt
      301 ttcgtcctcg gcggcttggt cttcgtcgcc atcctggcgg cgattctttc gacgatggac
      361 acggccgtca acgccggggc tctcgtgttg aaccgagacg tgatcgagca gattcatccc
      421 cgggctaaac gccggcccgt tttctggggg aggacggcga cggtgttgat cgcggccttc
      481 gcttttctgg tcgccctgaa gctccgtaac atcctcaaga cgatcgggct gtcgtcggag
      541 atcctggctg agggattttt catccccggg gcggcgatga tgtttttaaa agcaaaatat
      601 ccggcggccg ggcttttcag cgtctgcggg ggagggggat tcgccgtttt gagcttcctc
      661 gaatcctcgg gaatcgtgtc tttgggcctg ccggcctggc cgtactccgt cccctggggc
      721 ttggccgccg gggccgcggg attcgccgcg ggtttttggg tcgaccgacg gcggccggac
      781 cggctggcat gatcgatatt gttcaaggcc ggcgttttcg ctaagatggg ggacgatgtc
      841 cgccaagacg aaattccggc tgtggctcat ccagcacccg gggaagttcc tgatatggat
      901 gtggatgaag acgtgccgct ggaagatcgt cggagacggc ccgtaccggg ccctgaggaa
      961 gtcggggcgg ccggtcgttt tgctcatttg gcatggaaaa atcttcgttg tcccgttctt
     1021 tttccgcaag cgaggaatcc tggccctggt cagcccgtcc caagacgggg aattcgtcgc
     1081 ccggctgatg gacggctggg gctacaccct catccggggg tccggctctc atccgatgaa
     1141 aaccgcctgg atcgagatgg tccgggaact gaacgaaggc ggcgaggtca tcatcgttcc
     1201 cgacggcccg aaggggcccg accgacaatt caagccgggg gcggtcaagc tggccgccga
     1261 aaccggcgcg gcgatggtcc ccttttcctt ttccgcccgc cggaaaaaga tccttcgcag
     1321 ctgggaccgc ttcgagatgt tcttcccctt tacgcggatc gccgccgttt acggcgagcc
     1381 gattgagatt ccccgcggct tgccgcccga gagccgggag gaggagcgtc tccgggcgga
     1441 acgtctcatg atcgaattcg acgcccgggt cgaccggttt ttcgagagtg gggcggaggg
     1501 ctaggggcga acgtccggcg gcgaggattt ctggaaccgc gaggccgttt ccttgttcaa
     1561 caggatgatg attgaataga tccccagggc cgtcccaaac gggacgttga acaggctcag
     1621 gaaggagacg acgagggtga ggatccggcc ccattcgtac cccttcagga ggccgtatcc
     1681 gccgatgatc ttcggcaagc cgatcaaggc caggaacgac cccaggaaga agccgatgaa
     1741 acggaggatt ccgggagctt ggggatcgat gtcgggaatg aacgagaccc cgaccaaaat
     1801 caaaaacagg aagccggcgg ctacgaggct cagggcgccg aagatgatcc agaggatgcc
     1861 gatgattttg atatggtcgt tcatggccgt tctcctttcc agtttcatgt acgaaaaaca
     1921 acgctccggg ttcactcctt tgacggaaag ctcatggcgg aggacgatga tcgcggcgac
     1981 ggcctcagcg atttcatcga tccggggaac tggctcatgg cggaattccg gccgcaagac
     2041 aagaaatacg gttgcgttac atccggccgg atcaattcca accgcatgat cacggcggcc
     2101 gcgggcacgg cggcctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccgt
     2221 tcggggggat ccagaagacc cgggtcaatc cttatgatcc ggcattgaaa ttcccgggca
     2281 aggagcggga gccgtccaac gggatggatt attccggagc ccggcattat ccgcctgacc
     2341 ggtatcgctg tctgagcccg gacccggtgc tcaaccgcga tgcggcccgc tccgacccgc
     2401 agccccggcc gctacaaaaa tagtaatagg attcgtttcg tttaaaagca aagataatca
     2461 ccttgtctta agacacaggt tcagcgtctt gacaaacgtc cgctcaaaag ataaatctgt
     2521 atcgtttttc atcatggagg gaagaaaaat gaagaaattc ctgtgcttgt ccgttgccct
     2581 gctgctggcc ttggccgtct ccgccccggc ccagaccgcc gtcgagctca tcaaggccgg
     2641 agatgacgcc cgcgaccaga gagacgacgc gaaagcgctc gaatcctacc tggcggcgct
     2701 cgagctcgag ccggaaaact acgaagcgct ctggaaggcg tccggctcga tggtcgacgt
     2761 cgccgacctg gtcaacgtcc gggccaaggg cggccttgag aagcagaaaa agtattatca
     2821 ggacgcggcg gaatacgccc ggaaggcgat cgccgtgaat cccaatgaca cgaacggtca
     2881 tttccagctt tcggccgcgc tgggaatgta tgccttgggt ttgggcaaca aggaaaaaat
     2941 cgcgatgtcg agggagatca aggccgagat cgaaaaatcc atcgagctcg acccgaacaa
     3001 cgacggcgcg tatcacgccc tcggccggtg gcatcggacg atcgccgaaa tcggcggcgc
     3061 caagcggctg ctggggggca tcctgtacgg caagatgccc aagggaacca acgaagaagc
     3121 gctgacgtac ctgataaagg ccgtcgagat caagcccgac gacatccatc atcatctgga
     3181 gctcggccgg acctacgtcg tcgtggagga ctacaaggaa gcggccgcgg aattccggaa
     3241 gtgcgtggac ctgcccgagg cgtcgggcaa ggacgcgatg tacaaacagg aagcgaaagc
     3301 ggaattaagg aagatccaga ataagctgaa ataattattc gtcgaagact tccgcctcga
     3361 agatctcgat ccgggcgcct tttttccagg cgtcgggggg aagcccggcc ttcaaacagc
     3421 cgtggctgag aaacgtttcc cgcttccacc cgtactccac cgggacctgg ggaagaagga
     3481 ggcccttgcg ccctccttgg gagacgatga ttccgtgacg gccgacgacc acgtcctccg
     3541 gcttttccgc cgggacggga agcgtcagga cggagatttc gatgtgaagg tcgtccatct
     3601 cttcggccgt cacgggctcg aaacgcggat cctcgaaggc cgcggccgcg gccatttcca
     3661 cgaccgcatc ggccagcggc ttgaggggga gggggtagcc gatgcagccc cgaagctccc
     3721 cccggcgggt cagggtgacg aacgcgccgc gcttttcggc caggagcggg tcctttgttg
     3781 tcagggccgg cgtcggctct cccgtcaggc gggcggcgat cgattcccgg gctaagtgga
     3841 gaaggtcccg gcgtttttcg atcgagagag agccgttcat gggcgttttc cttccggccg
     3901 gccaaccggc cgctcgacga cgacgcggat tttcttgaag agaaaccggt cgtggtaatc
     3961 gatcaacgat ccggcggggg gccgcttttt cccgcccggc aaggggattt caacaagaac
     4021 cttgccggtt ccaaacggcc ggagaagggg cgcgcgggcg gtcgccggcg cgccgatgag
     4081 gaaaaaaccg acgattttcc tccccagtcc ggcatccatt tcataaaagg accggagcga
     4141 cggccaggcg gaggaggggg aggggagaac ggcctcgaca tacacgcggt tgccgcggcg
     4201 atggccgaga agaaaacccc ggccgccttt tttcggaacg aggcggcgga ccgcttccag
     4261 catccgggcg gcgtcggacg taagaaatac gtccatcgac ggcttgatca aagacgtttc
     4321 cttcatgttc aaccgagggg gccgacggcc ttttccaccg cttccaggat ctcccccgac
     4381 tggacgaggg ctttcatctt gttgtgatcg acgtgcaagg gacggtcctc gtcgaggcgg
     4441 gcgacgtgtc tgcggatgac gcccttggcc gcccgggcgc cggcgccggg cgtgaacggg
     4501 cggaaatcga gaccctgggc ggcggccatc aattcgattc ccaggatgcc gtaggcgttg
     4561 tccaggatct ggaggttctt cagggccgtg ttcatcccca tcgagacgaa atcctcctga
     4621 tcggcggccg ccgggatgga ttggatgcag gccggggcgc tgaggatccg ctgttcgacg
     4681 accagggcgt cggccgtgta ttggctgagc atcatccctg aaaaaagacc cgcgcccttc
     4741 gtcaggaaag ccggcaggcc gacgctcaag gcggggttgg tcaggcgatt gagccggcgc
     4801 tcgctcagga cgcagaccat ggtcaaggcc gctccggcca tctccatggg cagggcgacc
     4861 ggagtgcctt gaaaattcgc cccggtcagg gtgagcttgt tttcagtcag gaagatgggg
     4921 ttgtcggccg tggcgttgag ctcgatttcg acttggcggc gggcgtggac caaggcgtca
     4981 tgagccgccc cgatgacctg gggcgtggac cgcatggagt aggcgtcctg gaccttggtt
     5041 ttcattttgc cggtggttaa gtcgcttccc tggagacact tgaggatggc ccgggccgac
     5101 cggacggccc ccgggaagcc gcgctcctcg tggagccgga cgtcgtaggg cttcaaattg
     5161 ccgatcaggg cttcgatggt catcgtcgcg gcgatttcgg cttgtttcag cgtccggtcc
     5221 atgtcgtaga gcgtcagggc cgatatggcc gtcaggagat tggagccgtt gatggccgcc
     5281 agcccgtccc gggcttggag gccggggacc gggatgccgg cccgttccag ggcgacccgc
     5341 ccggccaggc gctcgccccg gtaatacgct tcgccctcgc ccatcagaag aagggcgacc
     5401 tgggacatgg gggcgagatc gccggaagcg ccgacggatc ctttccggca gacgaccgga
     5461 gtgacgccct tgttgagcat ttcggccagg gtcagagtga tttccgggcg gcagcccgag
     5521 cggccccggg catggacgtt gatccggccg gccatcgccg cccggacatg ctcgaccggg
     5581 gccggatcgc cgatgccggc ggcgtggttg tagatcaggt acttctggaa ctggccgacc
     5641 tgctcgtcgg tgaggaccac ctcggagaac tctccgatcc cggtgttgat cccgtacatg
     5701 atctcgcccg ccttgatttt ctcttcaagc atctcccggc aggccttgat tttggcgatg
     5761 gcctcggggg ggacttccac cggctcgccc tcccgggcga tgcggacgag cttctccagg
     5821 gtcaggcttt tcccatcaag gacgacggcc atggggatcc tcctcgggag ttgcggtctt
     5881 ttccgcccag accatcaggg cgtagccgcg ggtgaagaaa agcagcgtat cgagcttcgc
     5941 cgcgagccgg agcaggggat agacgagctt gtaaatccgg aagggcttcg cctgagcggc
     6001 gaattccccc ggcgtcgccg gccggatatg cccgtcgcgg agcctggcct ccggcttggc
     6061 cgagaagaaa ttcacgtaca gggcgttgag cacgagctcc acgaattccg tgaaaaaccg
     6121 ggaatagaag gcgtgccggg tgatccggag gccggcccgg ccgagaagca tccggagggc
     6181 gggcaaagcg tagccttccc gcttgtgccc gtagaattcg agcttcaggc cgacggccgg
     6241 gcgtattttc tgcatgagga tgaacggccc cgtccggggg acgacgagga gcagcttccc
     6301 gccgggaacg aggacgcggg cgatttcgcc ggccgtcttg agatcgtcgt cgacgtgctc
     6361 caggaaatcg agacagaata cgcggtcgaa ggacctgctt ttaaacggca ggtccggcac
     6421 gcccatctgg acgaagcccc ggtcgaggag gtcccggctg gtcagaaggt tttcgaaatc
     6481 caggtccgta tgaatccacc tcccgccttt tttcttcaag aaccagctga ggattccctg
     6541 ggcgcagccg aggtcgagac accggtcgtc cggcccgaca tcgacctgct gttcgatcaa
     6601 atcgattttt tcccttttct tcagggaacg gcgggcgact tcgagttgcc aggggagtgc
     6661 gtgggccacg tccggcatta tatcccatct ccggccggcc ggaaaaccgg ttcttgtcca
     6721 acttgacagg ggcggagaat aacccctatg atgaccctca ggacggagat cggatgaaat
     6781 accagatgat cgtggtcggt ccgctcgaga ccaactgcta tcttgtctac gatgaggaga
     6841 cccgggattg cctgatcgtc gaccccgggg ccgacgccga ccggattttc ccggtcatcg
     6901 ccgaggaaaa cctcaaaccc ttcgccattc tcaacaccca tggccacatc gaccacatcg
     6961 gggccaaccg ggacatgaag gacaagtacg acatccccct ttacatgcac gcgggcgata
     7021 aagaccgtcc ctcgcgtctc aaccagctcg agctgagcct tttcatcggg gccaaggatt
     7081 ccccgccggc cgacaagttc gtcaaggacg gcgacgagat caaggtcggc cgcggcgctc
     7141 tccgcgtcat ccatacgccg ggacattccc ccggcagcat cagtctcctg gacgacggat
     7201 tcctcctgtc cggggacacc ctcttcttcg agggtctcgg acggacggac cttccgggcg
     7261 gcgaccaaaa acagctcgaa aaatccatcc gggagcggct gatgaccttg cccgacgaga
     7321 tcgaggttct ccccggccat ggccccttga ccacgatcgg ccgggagcgc gaaaacctga
     7381 cttttctctg atgagcccgg ctccgccttc cgtcgacatc cgggcggaat tcctgcgttt
     7441 cctgaccgtg gaaaaaggcc tttcgcccaa cacgcgggaa gcctacggct tggacatcga
     7501 ccggttcctg cgattcctcg cccgcgaaaa aacgtcctgg gaccgggccc gggaatcgga
     7561 tctggcggcc ttcatccggc acgagagccg ggcgggcttg tcggcccgct cgctcgcccg
     7621 ccgcatctcc gtcctcaagg ccttctaccg ctttcttctt ctcgacggcc ggttggataa
     7681 aaatcccgcg attcacttga cgtcgccgaa aacatggctg gcgcttccca aagccctgtc
     7741 cgaggccgaa gtgaaatccc tcctgggaaa accggatccc cgggatcccc ggggccttcg
     7801 cgaccgggcg atgctcgagg tgatgtacgc ctccggattg cgggtttcgg agctggtcgg
     7861 gctgcggacg gccgacgtcc ggctgaagga tgagttcatc ctctgccggg gcaagggggc
     7921 gaaggagcgg atcgtgcccc tgggaaaaac ggccgcggcc gccgtcgtcc gttacgtgga
     7981 aaaagcacgg cccgccctgg cttcaaaaaa gggcgcggac gagctgttcc tgaccaggcg
     8041 gggagagccg ttcacccggc aaggattttg gaaaatgctg aaagggcatg cccgggaggc
     8101 cggcctccag ccgaaagtcc atcctcatgt cctgcggcac tccttcgcca ctcatcttct
     8161 cgagcacggg gcggaccttc gctccgttca gatgatgctc ggccacagcc agatcaccac
     8221 cacccagatc tatactcacg tcagccgcga gcgcctccgc cggatttacg acaagtttca
     8281 tccccgagct tgaccggtcc ggggaccgga gtccgcccgc ggagtcacga cgacggtcac
     8341 gtcggcctcg tgacgattca tttggaacaa ggcgggatcc cgtccgagga cgccggagcg
     8401 gcggaaccag gtccagtgaa atgatttttc ctgcgttttc cggctggccg cggggtcgtc
     8461 caagacgatc gcattggatc ctttttgaag gaagagggcg taattcaatc cttccggacg
     8521 gtcggtgttt tcggccgagg ccggaagatg gagataaaag gcctcggacg gccgagagcg
     8581 gggaacctcg aatccccgga tgagaaaatt caggtgaagg ccgccgtcct ttcggaggat
     8641 gtaggcgacg cccagctcag gcttgactcc gtaatcgcgg gtggtcagga gccggattcc
     8701 gtccggaccg gcggcggatt cctccactct ccattcctcg agtttcgtcc cgtggtggat
     8761 gagaacgtaa tcctcgtcgt cgggttcgag gccgcccctc cagacgaatc tcattccgta
     8821 gcgtccggag gcgccgtccc ggccctccag agcgtactcg ccctggaccg tgatcgtcat
     8881 ccggatgtcc caggcttggg ggggacaggg gagggcggcc ccgcccgagg cgagaaaaac
     8941 ggccgcggaa agcaggcaga ccgcagcgcc cgctttcagg atcttggccg ccggacgcat
     9001 gcggctccat tgtagaatga acggaaaaac atttccaatg cgaaggaaaa aatcctcttg
     9061 acaagcccct tctcttcttg ctatcctgcg ccggtatact cattggttta aagagtaaac
     9121 ttaagagttt aaaaatgaaa cctcagaaga gccggaagtc tttatccgaa aagcggagcc
     9181 tccgggagct cgaccgccgc cggaggaacc gggaggccat ccttcacgcg gccgagatcg
     9241 ttatcgggag gaaaggcttg acggcctcct ccatggacga tgtcgcggcc gaggccggtt
     9301 tttccaaggc gaccctttac aagtatatcc aaggcaaatc cgagctcgtc atggagctcc
     9361 tcatccatta cctggaggac ttgaacgacc ggctggccgc gatcttgatc aagccggtca
     9421 agcccgaggc caaacttctc cttcttctcc gggaaatcct ccgttaccag gcggagaaag
     9481 cgaacatttc ccgggcgttc atcatgaatc cgtctcacat gcagatcttc cgggcgatgg
     9541 ctgaaaccgg agcgatggcg gcctcggaaa cggaaaaaga ttttttccac cggcttcaga
     9601 cggcccgccg cgcgatgacc gggcacttgg agcggtttct ccgggagggg atcgcctccg
     9661 gggatttccg cccgtttccc ctggagatca tggtccgctt tctcaccgcc gtgattctcg
     9721 ggtaccagca cgaccagctg ttccgtgaat cgaaaccgga tatcgataaa gacatcaaga
     9781 acatccacga gttcatcctt cgtggattca aaaccgaatc acaggctgat tcttaaggag
     9841 acgcagccat gaaccgattt cgcgcattga tcgccctgat ccttctggcg gccgtttcgg
     9901 gcgggccggc cttggctcag gaaaaaatgg tcctgaccct ggaggactgt ctgaaactcg
     9961 ctcttgccca gaatcccttc tacctggccg cccgggccaa ggaagaccag gccgaggcct
    10021 ccctcaagga ggcggtcgcc ggatttttcc cgacgctcaa cgcctccggc accgacgtcc
    10081 tggataaaaa ggtgttcacg atcgagtttc cctcgttcat tccgggcgga tccccgacgc
    10141 gcgtcaagat ggatttcacc cgggcttaca ccttcgcctt gagcttcagc ctgcccctct
    10201 actcgggcgg acggctggtc gccggatata aaaccgccaa ttacaacctc gaggcgaccc
    10261 gcgaagccat ccgccaatcc cgggaggaga tggtcctcaa cgtcaagaag tctttttacg
    10321 gctatctcct ggcccgctcc ttcgtcggag tggccgagga aggcgtggcc ctggccgaga
    10381 agcactatac caacgtcaag aatctcgccg aggtgggcat ggcctcgaag ttcgaccttc
    10441 tccggtcgga agtccaattg gccaacctca agccgcagct catccgggcc cgcaatgccc
    10501 tggccaccgc cgaaatcggc ttgaaaatgc agctcggcct cgatctccag aaatccgtgg
    10561 aattcaaggg cgagctggct ttccgggacg tggaagccga cgtcgaccaa aatatcgtca
    10621 tggccctggc ccgccggccc gagatcaacc agctcaacta ccagaagctg atggccgcgg
    10681 aaatgatcaa gctgaacaaa ggcgccggcc ttccgagcct ggctgtcggc ggcgcctaca
    10741 actactggtc cgatcacctg aatctcaaga aggaaaactg ggaaagctat taccagatca
    10801 atctcgtcct caacattccg atcttcaacg gtttcggcaa cgcggccaag gtcgcccagt
    10861 ccaaggccgc cttccggcag ctggaataca gccagaaggg gctgatcgat tccgtgaagt
    10921 tcgaggtcca ggaagccgtc ctggccttga ggcaggcccg cgagtccctt ttttcccagg
    10981 aaaaaaacgt cgaacaagct caggaggccg tccggatcgc tgatttgaac tacgccgaag
    11041 gcctggccac caatctcgac gtcagttcgg cccaggtcgc tttgtcccag gccaaaacca
    11101 accatgttca agccttgtac gattatgccg tcgccctggc ccagatcgag aagtcgatgg
    11161 gaacaggctc ggatcgatat gaagaataaa cggagtcagc ccatgttgaa acatcgtttg
    11221 cccgccgtcc tgctcgccgc cgtcgtcgtt ttcgttccgg ccgcctgccg gaaaaacgaa
    11281 acgcccgccg ccgcggaagc cgcggccgcg tcccttttgc agggaaccat gccggtcaaa
    11341 gtcgagaccg tctcccggca gcggatttca gaaaagatga cgttcaccgg cgtcgtcgag
    11401 gccgcccgca agatcaatat cactccggag acgagcggca aggtcgctcg catcgtcgtc
    11461 gaggaaggcc agttcgtcca gaaaggccag cttttggccg agctggacac ggcttccatt
    11521 ctccttcagc tccagcaggc cgaagccgcg gcggccatgg ccgaggcgaa cgccaagaat
    11581 tccgcccgca acaaggagcg gatggaccgg ctgatggctg aaaacgccgt ttccgaaacg
    11641 cagtttgaac aagtcaagct cgccgacgag gccgcccagg cccaggcggc ccaggccagg
    11701 gcggccgtcg ccctgatccg ccgggccctg gatcaggcgg tgatgaccgc cccctggaac
    11761 ggcgtcatcg cctcccggaa cgccgaagtc ggcgacattc tcaatccgat gatgggcggt
    11821 tatggaagct ccggctccgg cgtcgtgact cttgtcgatt acggccgcgt caaaatcatc
    11881 gtcgaggtga cccaggacga catcctccgc ttggctcgcg gccagaaggc cgtcgtccgc
    11941 tccggctcgg cggaagcggc cggaatggtt tccgtcgtga acctggcggc cgatcccctc
    12001 tcgaaaaagt tccgggtcga gatcctggcc gacaatcccg ggctcgtcct ccggccgggc
    12061 accttcggat ccgtcacgtt cgaagtcaac tcccacgaga acgcattagc cgtccatcag
    12121 aaagccgtct tgtccgattc gtacgtgttc acggccgaaa acggcaaagc ggtcaagcgg
    12181 ccggtcaccc tgggcctcaa aaacacgacg atgatcgagg tcatcgacgg tttgaaggaa
    12241 ggcgaccagg tgatcgtcga gggcaacttc ggccttgtcg agggaacgcc ggtcgaaatc
    12301 aaaaggtagt catccatgca actccctaat tttgccgtca aacgccgcgt ttcgacggcc
    12361 atgatcgccc tgatcctggt cgtcgtcggg gtgatttcgt tctcgagact cgggctggat
    12421 ctctttcccg acctcgaata tcccaccgtc tccgtcgtca cgacttatcg aggcgcctcg
    12481 ccggaggaca tcgaaaacct catcaccaag cctctggagg gcgtcgtcgg ctcggtcaac
    12541 ggcgtcaaga aggtcacgtc gatcacgtcc gaggggacgt cggcgattca ggtcgaattc
    12601 gaatggggga ccaacctcga tttcgccgcc caggatgtcc gagaccgaat cgggctttac
    12661 aagggcttcc tgccggaagc ggcgagcgat ccgctcgtgg tcaagttcaa catggcccag
    12721 attccggtca ttttctgggg cgttgtcggc gatcgatcca tgtccgagct gaaaaccatc
    12781 gtcgaggacg aggtcgcccg gaggttcgag cgcctcgacg gcgtcgccgc ggcgacgacg
    12841 tattccatgg acacccggga aatcctggtg gacgtgaaca aggacgccct ggcttccttc
    12901 ggcctggcgt tcgaccgcgt cctcggggcc ctgatggccg gaaacatgaa catgcccgcc
    12961 ggctatttgg tcgagcggca ttcggatttt ctcgtccgga cgatcggcga gttcgagtcg
    13021 ttggacgatg tccgcaacac gattgtcggc tccacggcca agggccagcc gatttacgtc
    13081 cgggatgtcg ccgacgtccg ggatactctg aaggaaaccc gctttatcgg ccggatcgag
    13141 ggcgagaagg ccgtctatct catggtcagc aagcggtccg gagccaacac cgccctggtc
    13201 ggcaaggccg tcaaggccga gctcgagaac atcaagaaaa ccctgccccc cgacgtcaag
    13261 ttccttcaga tcatggacca gtcggagatg atccagcacg tcaccaaggg caccgcggcg
    13321 acggcctggc agggcggctt gctggccgtg gccctgatct tcttcttcct gatgaactgg
    13381 cggccgacac tgatcatcgg cgtggcgatt cccttgtcga tcatcacgac gttcatcgcc
    13441 ctttatttcg ccggctacac cctgaacctc ctgaccctcg gcggcctggc cttgggcatc
    13501 gggatgctgg tggacaacgc catcgtcgtc atcgagaaca tgtaccgtca tatccaggag
    13561 ggcaaggacg ccctatccgc ggcccggctc ggggcctccg aggtcgggat ggccatcacc
    13621 gcgtccacct tgacgacgat cgtcgtcttc cttcccatgg ccttcgccac cgggctgacc
    13681 gggatgatga cccggggatt ggctttgtcg gtcgccttct cgctcgtcgc ctcgttgttc
    13741 gtcgccctga ccatcgtccc ggccatgggc tccgttctct tccagcggaa ggaaggagac
    13801 gcccaggcga ttcggaagac gatcgggcgc gacttcaccg gcgcccggaa caaataccgc
    13861 cgggctctgg agtgggccct tcaccaccgc aagatcgtga tcttcggaac gctgggcgtg
    13921 ttcgctctct ccctgttcct gatcccggtc ttggggacgg aattcatgcc ctcgatggac
    13981 cgaggcatgc tggttatgag cgttaaaacg ccggtcggga cgtcgctcga agaaaccaac
    14041 cggatcgtga ccatgattga aagcgtcatc cggagccgtc cggaggtcca gatcacgtcg
    14101 acccagatcg gctcctcggc cgaggaaaac gccggcgacg ccggtttcgg gaatcccacc
    14161 gggccgcacg aggctgtttt cttcgtcgga ttgaagccga cggaagagcg ccggctgtcc
    14221 gattcccagg tcgtcgagga aatccgcaag cgggttccca agttcgaggg cgtcaagttc
    14281 gaatccatgg acatgtcggc gatgatgatg ggcggagcgg ccgcccccgt cgagatcaag
    14341 ctgttcggca aggatttggc gaaacttcgg gaaatagccg acggcctggt cgtccggctg
    14401 aaggacgtcc ccggcctccg cgatgtgacc cattccctgt cccagtccaa gccggagtac
    14461 cacttgaaaa tcgaccggga taaggccgcc cgcttcggcc tgaccgtcgc ccaggtcgaa
    14521 tccgccgtcc agacggcgac cctcggccaa gtcgcgaccc gctaccggga agagggcgag
    14581 gaatacgacg tccgggtccg gttcaaggac gagttccgcg agacgatcgc cgccatccgc
    14641 gacatcccca tctccacccc gatgggaacg accatctcct tggaccaggt ggccgatgtc
    14701 cagccgggca ccggcccgat ccagatcacc cgcgacaacc agtcccgccg catttccgtc
    14761 accggcaata tcgtcggccg cgatctgggc ggcgtcgtcc gggatgttca gactcggatg
    14821 aagggtctgg acgagcaact gccggccgga tatttcgtcg agatcggcgg ctcctattcc
    14881 cagatgaccg atatgttcaa aaccttgggc tgggtttttc tcctggccgt tctcatggtt
    14941 tacatggtca tggcctctca gttcgagtcg ctgaagcacc ccttcatcat catgttcacc
    15001 atcccgctgt gcttcatcgg cgtcgttctc ggcctgctcg tgttcggcca cactctcaac
    15061 ctggcggtcg ggatcgggat catccttctg gccggagtgg ccgtcaacaa cgccatcgtc
    15121 atgatcgatt acaccaacca gctccggcgg gacgggatgg agatcaagga agcgatcctc
    15181 cagggggcgg tgacccggct gcggccggtc ctgctcacag ccttgacgac cattctcgga
    15241 gtatttccca tggccgtcgc ccgcgggcag ggcgcggaga tgcgcagccc cttgggcgtc
    15301 actctcctgg gcgggttgac ttcgacgacc ttcctgacct tgttcgtcat tccggtcatg
    15361 tacagtctct ttgaaagagt gaggttcaag aaaaccgcgg cgaaaaactg agacggatat
    15421 cgcgtccccc gcggcccgga tggaccgcgg gggatttttt tcgaagctcc cgcgtcgtta
    15481 aaaagaagcg cttcgatccc cgggggcggc gcggtcctaa gctttatacc tcagcagagc
    15541 ccccatcttg ccgtagtggt cgagccgcga aggcggggtg atgtacttga cgtcgcattt
    15601 ccggtggagg gccgattcga tcgcctcgtc gacgatatcg ggacgcgttt cggtcttgcg
    15661 ctcgcaggcc gggcaccgga gctcgtccaa aaagagcaag cggcagcggg ggcaatgctt
    15721 tccggggacg aaatggttgc gggtgacgac gagggtttgg acttcgccct tgttgagggc
    15781 cgccaggcag tcgcgcagac cggagcgggc ccgggtgccc ttttcgatct cgccgatgag
    15841 cattttcaga aggtcttctt cctccctttt tttcagcgtc cgctcaagat cctcggcttc
    15901 ccggaggacc acatcgagag gatcgatagg ccggactttg aggcggccct tgatccgttc
    15961 ccggacgaag gaatggagca tcccctcgaa atcgcggtag agggcgtcgg ggcagcccag
    16021 ccaaagcccg gcgaagccgt ctttcttcag gatgtcgaac gttgtctggg cgcatcgctt
    16081 gaggtgggcg cggacaaggg cctcgacgtg acgctcgatc cgcttggcct cctgacccga
    16141 gattccgctt ttgactttct tggggatgtc cgaggagaga ctggacagcg gatcgatgcg
    16201 gctcatgtac acgtcgtacc accgggcttc ccggcggtcg agcgtcagga cgagaaagcg
    16261 gcggcggtcc tcgagaatgc gggtgacggg ccggatgtaa ggattgcgat cgacgacgag
    16321 acggtccaac ggcgcctcgg gaaggtcgag cgcttcccaa aagccggctc ccgagcagga
    16381 ataagccgcc agccccggca cggtcgagga cagattgccg gcgaagtagg cttcaagggc
    16441 ggccagatct ttctccagcg aggattttat ttccctggac acgtccatgg aggcgatgtc
    16501 cgcccggccg ccggcgatca tgttcttcgc ggccagaagg atctcccggc gcgttcgttt
    16561 ggacaggttg gtgtcgagaa agaacgaagt cgtcagaaga gggccgccgt ggaagcggct
    16621 gagggcgacg atccggtcac ggaagggaga gggcatgaaa cctccttatc gggaatagcg
    16681 atgaacggct ggaatacggg aatggaaaag acgccgtcca agacgtgcga gagcgtcatg
    16741 tagattaaag ataaagacgg ggcggaggat tgtcaagcgg acggaaagcc gcttccccgg
    16801 tcctttacat aaagccaaag aggccttata ataaaaaacc tcccgagcgc ggaagtggcg
    16861 gaattggcag acgcgtaagc ctcaggagct tatagccgca aggctgtggg ggttcaagtc
    16921 cccccttccg caccatgtcc cgaatcgtcg ctaattcctt tctccaagtg tgtttttcca
    16981 ggccgcggca tttgtctttg agggggaaat ctgttattta acatgggctt gggccggccc
    17041 gggggaaccg cggcccgaag gagacgcgtc catgagaaaa accgccgttt ccgcgatgat
    17101 cgggatcctg ggagcgggtc tcgtcttggg gctcgcggcc tgccggaagg aaggcccgcc
    17161 ggcgccgccg gtggccgcgg aaacaggaaa aggaggggcg aacatgagcg tcggcaagac
    17221 cgttttcgga acgctgcccg acggctcggc cgtcgatctt tacaccttgg ccgacgggaa
    17281 gggattgacg gtccggctga tgacctacgg ggcgacgctc gtttccctgg aatggcccga
    17341 ccgcgacggc gccctcgccg attgcgtcct cggctacgac acgcttgacg gctatctcaa
    17401 ggccagcccg tacttcgggg cgaccgtcgg ccggtacggc aaccgcatcg cccggggacg
    17461 attcgcgctg gacggaaaca cgtacacgct ggccgccaac aatggccaga accacctcca
    17521 cggcggtgtc aggggcttcg acaagatcct ctggaagggc gaacccgtcc aggattcgtc
    17581 cggcgtcggc gtccggttca ccaccctcag cccggacggc gatgaaggct atccgggaaa
    17641 cctggacgtc tccgtgacgt acgttctgac ggccggcggc gagctgatca ttacttatgt
    17701 ggcgaagacc gacaagccga ccgtcatcaa cctgacccac cacggctatt tcaacctcac
    17761 cggggggcgg cgcgacgttc tcgggcacga gcttgagctg gccgccgacc gctacactcc
    17821 cgtcgacgcc gagctgatcc cgaccggtct tctcgcgccg gtggaaaaca cgccgatgga
    17881 tttccggacc tccgtgtcca tcggatcccg gatcgccgcg gttccgggcg ggtacgatca
    17941 caattatgtc ctgaattccg gggggaagac cctggcctcg gccgcccggg tctttgagcc
    18001 ggagagcggc cgggtgatgg agatccggac cgtcgaaccg ggactccagt tttattccgg
    18061 aaattttctc gatggatcga tcgccgggaa ggccggccgg gtgtacggga agcactgggg
    18121 tttctgcctg gaaacccagc attttccgga ttcccccaat cgtccggaat ttccatcgac
    18181 cgtcctgcgg ccgggagaga cgtaccggac gatgacggtc ttcaaatttt cagcgaagtg
    18241 acgaacggcc gcggttgccg gccgccgtcg gaggagttgt ccatgagagt gcccgcgccc
    18301 gtccgattcc tgatcgttct gctggctttt tgggccgccg gcccggctcc ggcccaggac
    18361 cggctcaaaa cgtatcccgg ctatgaccgg tatgcggaaa tcaaggacca gatcaaggga
    18421 gcggtcaaac cgggaaccgt ccgggcggcc tgggaggacg gcggcaaggc ttttacattc
    18481 acccgcggcg ggaaaaggct tcgcttcgac atccgccgga aaacgctgac cgagctcggc
    18541 cccgcccctg aaaaagccga ggaaccggtc cgccgccggc ccggcggcct ggagcgaggc
    18601 cgtcaattca ccgaggctct ttcgcccgac gggaaaacca aggcttttta ccgtgaccga
    18661 aacctctgga tttccgatcc ggacggaaag aacgaacagg ccgtgacgac caacggcgac
    18721 gaaaagctcc ggacgaagtg cggctcggcc agctgggtgt acggcgagga gctgaaccag
    18781 atcacggcca tgtggtggtc gcccgattcc accaagctgg cgttttaccg cttcgacgag
    18841 agccgggttc ccgattatgt cctccaggcg gatcagaccc ggccctattc ccgggccgac
    18901 gtcgaggctt atcccaaggc cggccggcct aatcccgttg tcgacgttct tgtctatgag
    18961 ccggcggcga aaaaaaccac ggtgatcgac gtccgcgacg gccggccgtt cgaaaacgac
    19021 gtcgtcggcc attatgtcta caacgtctcc tggtcggtcg attcccggga gcttcttttc
    19081 ttccggatga accgcctcca gaacgtcttg gaattcgcgg ccgccgatcc ggtcacggga
    19141 aaatgccgga cgatcatccg cgaggaatgg ccttccggct gggtcgacat cgctcccgag
    19201 ctggcctggc tgaaggacgg ccggcgtttc gtctgggcct cggcccggac cggttttaaa
    19261 aattacgact tgtatgattt gtcgggaacg cggctcgccg tcctgacccg gcatcctttc
    19321 gaggcggccg gaatcgtccg gatcgacgag aagagaaaca gccttgccta tctggcccgc
    19381 gacggggcca acgaaatgat gctccagctc caccgggttt cactcgacgg ccggaacgac
    19441 gtccggctga cggatccggc ttaccatcat accgtggact tttcgccgga cggccggcac
    19501 ttcatcgaca cctgccaggc ccacgatgtc ccgccgttca gccgcctggt tgacgcccgg
    19561 gggaggatcg tggccgatct tgtccggagc gagacggcga agttcgacga gctcgggctt
    19621 aagaaggccg agctgttcac gttcgccgcg gccgacggaa cgacgccgct ccgcggcctg
    19681 ttgttttttc cctcgcgctt cgatccctcg aagaaatacc cgcttctggt gagcgtgtac
    19741 gccggcccgg aaaccaacgg cgcccgggag acattcgccg cgccgagcgc cctggccgag
    19801 ctcggcttcc tggtggcttc cttggattcg cggacggccg gggggagagg ccggcgcgcc
    19861 ctggacgccg tttacggcaa gctgggcatc gtcgaggtcg atgaccaggc cgccggggtc
    19921 gccgccctcc gcgcccggcc gtacgtggac ggaagccggg tcggcatctt cgggacgtcc
    19981 tatggcggat atgtctcggc gatggccctc ctccggcatc cggaggtctt cgccgcggcc
    20041 tgcgccatgt cggccgtcac gacgtggctt cagtacgaca gcatctacac ggagcggttc
    20101 atggggcttc ccgaggagaa caaggcgggc tacgaggcgg gcgaggcgat gcgctatgtg
    20161 aacgatctga aaggccggct catgatctat tatggaacgg ccgacaacaa cgtccaccca
    20221 accaatgcca tgctcctgat caaggccctc caagatgcgg ggaaaagctt cgatgtccag
    20281 atcgggcccg atgaggggca cacggccctc aaccgggagc ggatgatgga attcttcatt
    20341 gaggctctcg tcctgaagtg aacttttcca gccccttttc gtttaaggag atgggagaaa
    20401 acaaatgaac tcgtcttcgg cgaaaacctt gggcatcgtt ctcgtcatcc tggcggtcct
    20461 catggccgcc ggccttcttc gggcgctgat cctgatcccc ctgggaatcg ttgaaggatt
    20521 cggccacggc cttcggcact ggggcgggac ctggttctgg ccgtggtttg gcctcaccgg
    20581 cttcttcggc ctctttttcc tggttctctg gatcatggtc gccgtttggg ttcacggcga
    20641 cgcggaacgg aggggaatgc cggggatcct gtgggccgtc ctcgtgtttt tcacccacct
    20701 tctcggcttg atcatctacg cgatcgtccg ctcaagcaaa tccgtcaagc gggaagggcc
    20761 gccgtcggcc ccgcccgccg agcccgagcc ggccgctccg gggaatccgt ccgccgagtc
    20821 tccgcccctt atcatgccgg ccaagccggc ggcctgctcc cggtgcggaa aggaaacccg
    20881 gcccgaatgg cggatttgtc cgttctgcgg ggagaaaatc tagcttattc acgggccgat
    20941 aggaacgcgt ctattttgcg gcggggagtc agccgcggga ttgacacggc ttcgccctcc
    21001 gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncctccgc ggttttattg
    21121 actcgcaaat catccggaca agaaagaaaa aaatcatttt ttagggttga ctaaatattc
    21181 actaagtgat atattagata taagaaagga taacttgttg taaatacagt aattagaaaa
    21241 catcatggcc tctaaaaaaa acttagtttc gaatgaatcc ataaaaggat tcattttcga
    21301 tatcaagcgt tttgccgtgc atgacggtcc cggcatccgg acgacgatct ttttcaaggg
    21361 atgcccgctt cattgtctct ggtgccacaa tccggaaggc atcgaccggc gtccggagct
    21421 tctcctccgg tcggcccggt gcgcgcgttg tcacgcctgc gtcaaggcat gtccgaaaaa
    21481 cgccttgtcg cccggggcgg acggagatcc cgtgaccgtc gatcgttccc tctgcgaccg
    21541 ctgcggagca tgcgtcgacg cctgcgctta cggcgccctg gaaatcgccg gccgtcaaac
    21601 gacggtcgcc gatctcgtcg ccgaggcgga acgcgaccgc gtatttttcg agtcgtcggg
    21661 cgggggagtg actctttccg gcggcgaacc gctggcccag ccggagttct ccttggccct
    21721 gctggaagcc ttgaaagttc gcggattggc cgcggctctc gatacctccg gggcggtgga
    21781 gggggacatt ctggaccggg ccgcggatct ggccgacctt attctctatg atttgaagac
    21841 catggatgag gcggtccatc gccgttttgt gaaaacggga aacgcccgca tcttggagaa
    21901 tctgcgccgg ctcaaccggc gcggccgggc cgtccgggtc cggattcccc tggcggccgg
    21961 ggttaatgac ggcgaggaga acatcgacgc ctcgatccgt ttcctgaaaa ccctggcctc
    22021 cgtccgccgc gtcgacttgc tgaagtatca ccggggcgga gaagagaaat accggaacct
    22081 cggccgggcg gcggacttcg agattttcga gcctccttcc gatgggcgga tggaggacat
    22141 ccggcgggcg ttcgccgagg ccggattcaa cgtttccatc ggaggttgat cgtgatgacc
    22201 gagcgggtga aaaagctgcg ggagaggagc ctggcggcgg ttccttcgat ttccggagag
    22261 cgggccgccc ttctcacgtc cttttatcgg agccccgagg cgggccggtg ttcgccggcc
    22321 gtggcccatg ccctggcctt caagcggatc atggctgaaa agaccgtctg ccttgaagac
    22381 ggcgagttga tcgtcggcga acgcggtcca gcccccaagg ccacgccgac gtatcccgag
    22441 gtcaattgcc acagcgtcga ggatctccgg atcctcgact cccgccctaa aaccttcttc
    22501 aaatgcgatc cctcggtcat ccttttgtat gagaaggaaa tcatcccgtt ctggcgcggc
    22561 cggtccatcc gcgaccggat gatggaggtt ctcccccccg cttggaagga cgcctacgcc
    22621 gcggggatct tcaccgagtt catggaacag agggctcccg gtcataccgt cctcgacgac
    22681 aaaatttaca aaaaggggat ggaggacttc atcgccgaga tcgacgccga aacggcccgg
    22741 ctggattttc tcgccgatcc gctggcctat gacaagcggg aagaactgcg ggcgatgcga
    22801 atctccgccg aggccctgat catccaaggc cggcggcacg ccgagaaagc ccgggagctg
    22861 gccgcccggg aggggaacgc ggcccggaaa aaagagcttc tccggatcgc cgaagtctgt
    22921 gaccgcgtcc cggcccgcgc tcctcgggat ttccaggaag ccctccagat gtactggttc
    22981 gtccacctgg gagtgatcac ggaatacaac acctgggact cctttaatcc cggccgcctc
    23041 gaccagcatc tttatccgtt ctaccggaaa ggcctggagg aggaaacgtt gacggaggaa
    23101 tccgcccggg agcttctcca gtgcttctgg atcaagttca acaaccagcc ggctccgccc
    23161 aaggtcggcg taacggccga ggagagcggg acgtacacgg acttcgccct gatcaacctg
    23221 ggcggggtga aagccgacgg ctccgacgcg gtcaacgagc ttacgtatct tctcctcgac
    23281 gtcatcgagg aaatgcggct cctccagccg agctccatgg tccaaatctc caagaaaagt
    23341 ccggagaagt tcctcctccg ggccctgaaa atcgtgaaaa ccggcttcgg ccagccgtcc
    23401 ctgttcaaca cggacgccat cgtccaagag ctggtccggc aaggaaaatc cgtcgaggac
    23461 gcccgaaacg gcggtgcttc gggctgcgtc gaatcaggcg ccttcggtaa ggaaaattac
    23521 accctgacgg gatacttcaa catacccaag gtcctggagg tggccctccg agacggcttc
    23581 gatccccgga cgggaaaaca gctcggcccc cggaccggcg atccggcggg attccagacg
    23641 ttcgacgacc tcttcagcgc cttccaagcc cagctccgtc atttcatcga cgtcaagatc
    23701 gccggcaacg cggtcatcga gcggttgtac gcgactcttc tgcccgcgcc gtttctttcg
    23761 atcctgatcg acgattgcct ccgccggggg cgggactacc acgccggcgg agcccgttac
    23821 aacacctgct acatccaggg cgtcgggctc ggatccatca ccgacatgct ggcctcgctc
    23881 aagcacaacg tcttcgaaaa gaaaatcgtc acgatgaaaa atctgatcgc cgccctggac
    23941 gccgacttcg ccggggccga ggatctgcgc cagcggcttc tcaataaaac gcccaaatac
    24001 ggcaacgacg atgacacggc cgacgaccac gcccggcggg tctttgaggc gtattttgcg
    24061 gccgtcgacg gccggcccaa catgaagggc ggctcttacc ggatcaacct tctcccgacc
    24121 accgttcatg tctacttcgg caaggtcacc ggagcgacgc ccgacgggcg gaaagcgggg
    24181 gaaagcctga gcgagggcat ttccccggtc cagggttccg accgcaaggg tccgacggcc
    24241 gtcttgaaat ccgcggccaa gatcgatcat atccggacgg ggggaacgct gctcaatcag
    24301 aagttcacgc cgcagcttct ggccgacgag gaaggtctcc gcaatctggg gcatcttgtc
    24361 cgggcgtact tcacgatgga cgcccaccat gtccagttca acgtcgtcac ggccgaaacc
    24421 ctccgcgacg cccaggccca tcctgaaaaa taccgcgatc tgatcgtccg ggtcgccggc
    24481 tacagcgatt acttcctgga ttgctcggtc gacctgcaaa acgagatcat ccgccggac
//
LOCUS       DAOOXH010000030        24264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_1887_length_24264_cov_8.089388, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000030 DAOOXH010000000
VERSION     DAOOXH010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 24264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24264
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1887_length_24264_cov_8.089388"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1676)
                     /locus_tag="PKV64_05215"
     CDS             complement(<1..1676)
                     /locus_tag="PKV64_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPN16516.1"
                     /translation="MAQRPISTFVPVNAETIRQIRDIVGRFVHTIAAMRLYPFGHVNV
                     TKFRDNLFQRLSAFLEEQGEFEIEIKEDAFTFAGGPVFQEESGIKSLPYLYYNDGMRA
                     LTFLPGLTREELFDFLAITADYAERAEEGDIIEDLWERDFEFIRYDAADGFLEARVAA
                     GTVHRPKELQIDREHLFSGRIQLDAEDKAAAHARSVEISRTNPLDGSVLAEFFRSIDG
                     GERSNLESLVSAEHEVSVDKEFIATVFEVLYLEDRLDSFRQILTYMKTYLKILIKKAD
                     IGNALFLLNSISDLRKALLASSPARAEDLLQTIRLITQETDLDIIRDIARPERIEDPR
                     PLFEFLVWFGPRTLPTAIEACEAAVDPLWREACVEYLRVMVRDCLSETAAQIRNRKPE
                     LIQTLITLLGDRGDKKAILHLADLARSGNPEIRLEAVRTLGGLSHDLARKLTLEFLLD
                     PDERIRIEAGQAARLESDENALARMIQIVSRKNFLARSSTERGAMLLALGRSNTGPAA
                     KALETLVRKRRLLGGERLRETRLLAVSGLAVMNIPQAREALAAGVKVSNQA"
     gene            1841..3193
                     /locus_tag="PKV64_05220"
     CDS             1841..3193
                     /locus_tag="PKV64_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HPN16517.1"
                     /translation="MNLEEKLKRLRMEREARSRGPASSVENTWSRLQREAGLSTKEKL
                     EQLIRLTGGTAGPRPKKSVPREPEPRPPLQVFENAYPPDARYGKLRISDALAVGGEVL
                     RYLSRDEAFRDLDLSTTVFLDLETTGLAGGTGTLPFLVGLGYFRERAFIVAQYFLGDP
                     GEEERMLEELSRLFAERRFKSVLTYNGKAFDLPLLETRFILQRKSFSLAGLPHLDFLF
                     AARTLWKHKHESCRLFHLAQQVIEAPRDEDIPGAEIPYRYFDYLRTGDFERIEPILYH
                     NQEDILSLLGLVISGARLFSEEDLDGETGPDPLDLVGIGKVFARAGEAARSTRLFERA
                     LVGRLPSDVAVSVVRRLTSQLKKEADWERAVALWRDGSAADHVFCYRELAIYYEHKAR
                     NLAEAKRITEEGLALAMERSSAERVDFEKRLARLNAKLQKSGPVKKKIRSHRGSGEAS
                     "
     gene            3285..3358
                     /locus_tag="PKV64_05225"
     tRNA            3285..3358
                     /locus_tag="PKV64_05225"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3319..3321,aa:Arg,seq:acg)
     gene            complement(3523..4005)
                     /locus_tag="PKV64_05230"
     CDS             complement(3523..4005)
                     /locus_tag="PKV64_05230"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPN16518.1"
                     /translation="MKLKTLVGSGDKIARSVLPVIIIGLALNVWRPSIFSVGGPSPVL
                     KIVSIALLVPGVVVWARSVALILRKVPRKELITTGPYAIVKHPLYTGAAFFVLPWLGF
                     LFNSWLGVAGRDRPLCRIPGVFASRGGRSGQDVRPRLRRIPSQRQAALAIGMPAVDRL
                     "
     gene            4167..4850
                     /locus_tag="PKV64_05235"
     CDS             4167..4850
                     /locus_tag="PKV64_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HPN16519.1"
                     /translation="MPLISPVPVQLDPGDVVRVLRLDRRPGGKRAAGPLLAEAASLLR
                     PRAFYREAFIESRSAEGLTIGGRFFRSRLLRGQAGRALKVFAFVLTIGPELEEAAVRA
                     KDLLHRYDLETLADMAVEAAAAALGERLAGASGLGRLAALSPGTVEDWPVTEQVPLFA
                     LLGDGPGRAGVRLTNSMMMIPRKSVSGLLFPSKETFLSCRLCSRRSCPSRKAPPDPEL
                     RRRLGDRKA"
     gene            4961..5176
                     /locus_tag="PKV64_05240"
     CDS             4961..5176
                     /locus_tag="PKV64_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HPN16520.1"
                     /translation="MKKEFTAVSIPTSLYKEIEEAIRGTADPSVPNYVARVLRESLKK
                     TGSQEDVFSPEDEEKVKDRLKALGYID"
     gene            5247..6395
                     /gene="sat"
                     /locus_tag="PKV64_05245"
     CDS             5247..6395
                     /gene="sat"
                     /locus_tag="PKV64_05245"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HPN16521.1"
                     /translation="MIQPHGGQLIDRVLKGAALDEALKKAASLPSIPLDAEQTSDVEN
                     IATGVFSPLEGFLGEKDFRSVLDKMRLANGLAWTIPVILAVDRKTADGLAAGADVALL
                     NPAGEPAAILHLAEKYGFDKAETARKVFGTEDTAHPGVAKIMSLPDVLLGGSIDLIRE
                     TPTPFQKYKLTPRETRRLFQDKGWSTVVGFQTRNTPHIGHEYVQKSALTLVDGLFINP
                     VIGKKKKGDYRDDVILSSYEELIKNYYVPERTTMAILMMEMRYAGPREAIFHAIIRKN
                     FGCSHIIIGRDHAGVGSYYAPFAAQEIFADFPDLEITPLFFRSFFYCKKCGAVVSDKI
                     CPHGKDHQIQFRGTEIRDMLVRGEYPPAELVRPEVAKIIMDEKNPFND"
     gene            6420..7787
                     /locus_tag="PKV64_05250"
     CDS             6420..7787
                     /locus_tag="PKV64_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPN16522.1"
                     /translation="MRKALIIGLDSAPAEIVFDRRKEFPNLNRLLENGSYGTMRSSDP
                     PITIPAWMVMSTGKDAGRLGLYGFRHRTGPAYDKMWIATAQSINEPAIWDIIGQAGGQ
                     SVLVSVPPSYPPRPLAGNRIGCFITPGADKDYTYPKELKAEIEEKFGPYLFDVPFRIE
                     NRAEILKEIYRMTEKRFEVIQYLMRAKPWNLFMFVEIGVDRVQHAFWKFFDKTHHLYE
                     AGNEFEAVIVDYYKFVDEKIGALLKEAGDDTVVLAVSDHGAKRMKGAFCINEWLIAEG
                     DLVLGAVPARGASIEKTPIDWAKTKAWGWGGYYARVFLNVEGREPQGIIKPADYEKER
                     EALAERLRGIRGPNGEKWATQVIKPNEYYDDARGDYPDLMVYFDDLYWRSAGTLGWGT
                     MYLAENDTGPDDAVHAQHGMYIFYDPQAKVSTRKDIDITDIAPTVLKALGLPVPADMK
                     GRSLR"
     gene            7811..8353
                     /gene="cysC"
                     /locus_tag="PKV64_05255"
     CDS             7811..8353
                     /gene="cysC"
                     /locus_tag="PKV64_05255"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HPN16523.1"
                     /translation="MCDHQHAKGVTLWFTGLPCSGKSAVADRAAEILKNGGYNVERLD
                     GDIVRQDLTRDLGFSKEDRDENIRRVTFVAKLLTRNNVIVLTSFISPYKAMREHARKE
                     IGSFIEVYVNTPLETCIQRDVKGMYKKAMAGQIKEFTGISDPYEVPENPEMILECGAE
                     PLEESARKVIQKLKDLGFVR"
     gene            8389..9333
                     /locus_tag="PKV64_05260"
     CDS             8389..9333
                     /locus_tag="PKV64_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPN16524.1"
                     /translation="MSRYLVTGGAGFIGSHLVETLLDRGEEVRVLDNFLTGKRENLAS
                     FGGRLEVVEGDLRDPDACRRAVDGVDFVLHEAALPSVPRSVADPFTTDEINVRGTLNL
                     LWAAARAKVRRLVFASSSSVYGDAPGLPKREGEEGKPLSPYAASKWAGEKYLQVFAKT
                     FDLETVSLRYFNVFGPRQDPASQYAAAVPLFITRILRGEAPTVFGDGEQSRDFTYVAN
                     VVEANLLACTAGGAAGGVFNVACADRITVNVLIAKINEILGKSIAPVYAAPRPGDIRH
                     SFADISAAERELGYRPVVGFEEGLRRTIAWCGQRGNHR"
     gene            9330..10649
                     /locus_tag="PKV64_05265"
     CDS             9330..10649
                     /locus_tag="PKV64_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPN16525.1"
                     /translation="MNTQSRALMRKIKTKEAAIGVVGLGYVGLPLVKAFLNKGFKVTG
                     FDVDRKKVRLLNKGQSYIKHVTATELKKYLAVRRFEATDDFSRLKEMDAILICVPTPL
                     DAHRNPDLSFVLGTTETIAAYLRKGQLVILESTTYPGTTDEEMLPILERKGLKGGKDF
                     FLAFSPERENPGDPVFSAENTPKVVGGLTPDCLALADALYSQVVAKTVPVSSTRVAES
                     AKLLENIFRSVNIALVNELKLIFERMDIDVWEVIRAASSKPFGFMPFYPGPGLGGHCI
                     PIDPFYLTWKAREVDYQTKFIELAGEINTKMPYYVVNKTMEALAARGKSLKGSRILVL
                     GIAYKKNIDDQRESPSLKIISILRAKDVRVEYNDPYVPESSGHREYPGLKLKSVPLTA
                     ARLRAVDAVIIATDHSCYDYDFIVKNARLVIDTRNAVKTPRKNVIKA"
     gene            complement(10712..12319)
                     /locus_tag="PKV64_05270"
     CDS             complement(10712..12319)
                     /locus_tag="PKV64_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16526.1"
                     /translation="MRTSKRKWVVPLILFAGSLIFNLTLNKSIILDGDEAALVMKSVG
                     TLNGELPLVDFVGFTYPPGRYFLLALLFTVFSPSVAVERLFFVVLRSLVNVLMYLFAT
                     KLLPKGWTFLPVAMIGVMACFPWKTLLPFVTLLNLIFLEKLAAAPLRKKALLSSAVVA
                     GFTLGFRQDIALYAWAAAALLIAWQVYEALPETESGRFSAPAFLKGNLKHQSRYFLTM
                     AGVFSPLAAFYFLKSHGIKLLYEMFIGRPADHLRVVAHYSHHFPSLGKTLQSPLDGEK
                     IFLWIFLLILPATAALLLTRVFRERRLTRENQTVLAILVIAVLSFSSETFVYAIFERL
                     LQGATALYLLAAYLLSLADSRLRALVRAKIRRPALRRAAAAGALALLLSGPVAFSVYG
                     LAVKNVNDRLTLARDKNAYIVSPSGIGSSRKRIQFVLHAIQAKLAQKNIIDDRDVLLY
                     AEGSLIYYTSTWAEARRLDFHLKPLKPSLLEADLGALRPRFLLIQNSALYVVKKFPER
                     LRRKIEDEYAPALQKSGFTVYRLRDGT"
     gene            complement(12582..13841)
                     /locus_tag="PKV64_05275"
     CDS             complement(12582..13841)
                     /locus_tag="PKV64_05275"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16527.1"
                     /translation="MTVTIRILSPSEHCLSRSDVDPDALRILARLQRMGFIAYLCGGA
                     VRDLLLGRTPKDFDVVTDARPGQIKKRFANVFIIGRRFRLCHVHFPGGKIIEVATFRR
                     DLSAEEQAAPPGKIPPELLYGTPRDDAFRRDVAINALYYDARTGEVIDYAGGLEDLAL
                     KRVRIIGDPAARFAEDAVRIWRVVRYAARLGFDIEEETARAIPVFAPLLGKCSGARLY
                     EELNKELVEKTRPVVDALRAHGLLRHIIGSFGEDYESDDALFERLRALLEAKDRLQKE
                     GVVWSREEAYALFFWPWAESFFAGKDGDIRAVLEKAFESAGLQAVFPRKVRTDVAHIL
                     TLVSQIMNVLRTGRPRWSLRRQALFESAVRIYGVIEDGRPPEPGAFPEKLFGRPRPSS
                     GEPEPRPEGKRRPRRRRRRKPHPPKAE"
     gene            complement(13838..15502)
                     /locus_tag="PKV64_05280"
     CDS             complement(13838..15502)
                     /locus_tag="PKV64_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16528.1"
                     /translation="MLNPSLRFFRPRPILPATALALWAAFAFPAAAPGQTTTTFRTSV
                     PEIRSLWHLIRATLEANRMSFPGKSGLISAFSAGSLYPQIWIRDAATTIQASRYYSPA
                     EEIVSWLEEFLAFQETGGGLPDWFDSHGRSDKNITETDQEASAILAAARAVEIHGPGW
                     LEKKIAGRPVLDRLDLALSFVLERRFDVKRGLVTGAHTADWGDLGMEEADQTAIYAGQ
                     STRWTCDIYDQSMFYGAARALAGMLRNTGRVERADFWTRRAESVREAADRYLWQESRG
                     FYRVHVHLDGPVHPFDEDDMFAMGGNAEAVVSGLAGPEKAVRIIQAALARQEAFRMPT
                     IGAALLPPYPKGTFKHPMVDDPYEYQNGGLWDWFAGRLVRAMFDHGYSGAARAKLLEI
                     ARKNIAAGGLHEWNAPDGSGRGSPVFAGSAGSLASAVAEGYFGVRLTRDGCRLEPRLA
                     EDGAKVFFRLPASGSAAAYEYSWNPAEQRVVFRYESPSSRPGWIRLLLPRALSEAELD
                     VRRDGRPVPFSIERLGEDVLLVLETDFASHTLVLRAKKTSQGSSRS"
     gene            complement(15496..16482)
                     /gene="hypE"
                     /locus_tag="PKV64_05285"
     CDS             complement(15496..16482)
                     /gene="hypE"
                     /locus_tag="PKV64_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HPN16529.1"
                     /translation="MDKISLELGGGGKTMRDFLARSILPTFPNARLAGLPDAASLPGG
                     IAFTTDSYVVDPIFFPGGDIGTLAVNGTVNDLVVSGAKPRFLSLALILEEGLETASLE
                     RILRSIRKTADAAGVEIVTGDTKVVRRGQGDKIFVNTSGIGRIIGRARPGAIRPGDRV
                     IVTGTLGEHSLAVLAARGEFGFENPVKSDCAPLTFLLPLWSRGVRWMRDITRGGLATV
                     LSELAEQSPCPILLEEKAIPLSTAAKSAAGLLGIDPLYLACEGRAVMIVPENRAEGVL
                     STLRGHRQGRRAAIVGRADKAPGRPGELLLRTESGGLRLLEPLTSELLPRIC"
     gene            complement(16475..17614)
                     /gene="hypD"
                     /locus_tag="PKV64_05290"
     CDS             complement(16475..17614)
                     /gene="hypD"
                     /locus_tag="PKV64_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HPN16530.1"
                     /translation="MASDLFRDRRVVAGLLSEIAAAAASSSPPVRLMEVCGTHTMTIH
                     RHGLKDLLAQAGVEMISGPGCPVCITPDGFHEAAIRLVTERENLILATFGDMTRVPTR
                     LGSLQAAVPAPGSAVKIVYSPEEALTLARLNPVKDVVFFGAGFETTIPAIVLTAGRAQ
                     AEGLKNIAVLTAFWTIPPPLRAIVSSGEIRISGFLYPGHVSAIIGLEPYAFVAREFGL
                     PGAVAGFEPGDILLGVRSVVEQIRAKKPAVVNAYPRVVRPEGNPAAREVMNRFLEPYD
                     ALWRGLGRIPASGLRFKPAHAAVDAAVKYALRPAEDASDSPGCRCGEILRGVISPPQC
                     RLFGRSCRPESPRGPCMVSFEGACYVHHKYRGSSPAQRRSRKSHG"
     gene            complement(17616..17846)
                     /locus_tag="PKV64_05295"
     CDS             complement(17616..17846)
                     /locus_tag="PKV64_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015492853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HPN16531.1"
                     /translation="MCLAIPVRVVEIDASGDAGKVDYLGTKIGANFALIRDVRVGDWV
                     IVHAGFAISRLDEAEARETLALLRDVQDAGRS"
     gene            17932..18705
                     /gene="uppS"
                     /locus_tag="PKV64_05300"
     CDS             17932..18705
                     /gene="uppS"
                     /locus_tag="PKV64_05300"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HPN16532.1"
                     /translation="MLKGLDGWLEDGSEEAGLALRLDAARIPRHVAVIMDGNGRWAAE
                     RGLPRVEGHKAGAKSVREIIETGARAGLDVLSLFAFSTENWKRPPREVQTLMRLLEET
                     LGKEDGLLMEHGLRLRVLGAKDKLPGGLRRELERVETMTRGNSRMIIGLALNYGGRAE
                     IVEAVRRILAEGRLAAGDLDENVFSRYLDTAGLPDPDLLIRTSGERRISNFLLWQIAY
                     AEIWITPVFWPDFRRKHLLQALVDYQGRNRRFGAAEPSA"
     gene            18702..19517
                     /locus_tag="PKV64_05305"
     CDS             18702..19517
                     /locus_tag="PKV64_05305"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPN16533.1"
                     /translation="MNIVKRFWTALVLLTALFLVLEYGNKWVLFAALQAVVIASLIEF
                     YNLARRRRLYPAAVPGILFALLLGASFLIEGLTLELALFAGLLGLGVFYVVVSRTIET
                     AVRTTASIAVTVFGPLYIGFTLNFLYPLRQTYGSAPIYFLLAVVFLADTGAFFVGKAA
                     GRHPMTPIASPNKTWEGAAGGLIFAGLTAWAARAVFLPEIGPGRAILTGLAVSIAAQA
                     GDPLESLFKRAAGVKDSSNALPGHGGFLDRVDSLLFAVPLFYFIVRYIWKPLG"
     gene            19514..20671
                     /gene="dxr"
                     /locus_tag="PKV64_05310"
     CDS             19514..20671
                     /gene="dxr"
                     /locus_tag="PKV64_05310"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPN16534.1"
                     /translation="MRKIAVLGSTGTIGRNTLDIVRRHRRLFKVTALAAGSNRDLLLS
                     QIVEFRPLLVSVGTDADAAALRRELGRRGPDILSGAEGASAAAAETSADLVVSAIAGF
                     NGLRPTLAAVRAGKTVALANKESLVAAGGLLRREAKNSGAVFIPVDSEHSGIFQCLAG
                     IDRGDIRSVVLTASGGPFLRIPLRELRRKTPEEALAHPRWTMGRKVTIDSATMMNKGL
                     ELIEARWLFDLAPDMIRVLIHPQSIVHALVELRDGSVLAQLSRTDMRIPIQYALTYPR
                     RRDGFLPPLDLAASGPLEFSSPDERRFPLLKTARRVMGEKDSAAVAMNAANEIAVEAF
                     LGARLRFNSIAETVEEALAGLRPRRLDSLDDIIEEDRRIRERTRRRIEKRI"
     gene            20673..22004
                     /gene="rseP"
                     /locus_tag="PKV64_05315"
     CDS             20673..22004
                     /gene="rseP"
                     /locus_tag="PKV64_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPN16535.1"
                     /translation="MGTIFGTILAFLIVFGILVFIHEFGHFFAAKLVGVRVEVFSFGY
                     GKRLFGFKKSGTDYRISLLPLGGYVKMLGEGLAEPGKILEPDDFMAKNRLQRFLVFVM
                     GPVMSILLAVVIMAGLNMVGVTTPRYQDEAPVIGWIEPGSPAARADLKPDDLILAVNG
                     RKTPTWNDVEIAVGTKPNRLLSLDIRRDGRVMEVPLTTESRTKYEMGYAGFFGKILTQ
                     VQMVSPGSPAEKGGLQAGDVILAINGEPVYFYRFVEIIESNPEKECAFRVDRGGREIA
                     LSITPRREGKVGKIGVMQTAQSVLKKFAFFPAIGQSLKENARLTFLVIDFLKNLFTGQ
                     ASAKQLSGPLEIANFSYAALKMGFLSLMSWIALISLQLGILNLFPIPVFDGGQIFVLA
                     IEGILRRDLTPKARQIWMQIGFAVFILLIGFVILNDIAKRLPNGWRSLLPF"
     gene            22310..23416
                     /gene="ispG"
                     /locus_tag="PKV64_05320"
                     /gene_synonym="gcpE"
     CDS             22310..23416
                     /gene="ispG"
                     /locus_tag="PKV64_05320"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPN16536.1"
                     /translation="MKEQGPPLFPRRRTVQIRIGNVAVGGGSPVSVQSMTKTDTRDVR
                     ATAAEIRRLAREGCEIVRLAIPDAEAARAFARLRKASPVPLIADIHFDHRLAVACLEA
                     GADGLRLNPGTIGTRDKVREVVRAAKERSVPIRIGVNAGSIEKDLRSARGGTTAKAMA
                     ESALRHIRILEDLDFRLIKVSLKASDLTRTLQAYRLLAERTDYPFHAGITEAGTLLPG
                     AVKSSAGLALLLAEGLADTIRVSLTAPAVEEVRVAYRILAAMGLRARGLDIVSCPTCG
                     RCEVDLASIVRQIHRRLAGVPEPLTVAVMGCAVNGPGEAREADAGVACGRGSGALFIK
                     GEILRRVPEKEIADALVREVVRIAAGRKDGDERR"
     gene            23403..>24264
                     /gene="larE"
                     /locus_tag="PKV64_05325"
     CDS             23403..>24264
                     /gene="larE"
                     /locus_tag="PKV64_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HPN16537.1"
                     /translation="MSVAEHPSVSRRLEEKVGRLRDILQGMDRVLVAFSGGTDSAFLL
                     KIAAEVLDRNVSAVIASSPVFPASEIADAERLARKFKVRYRIIRPAVFKNPRFVKNTP
                     LRCYECKKDLWRSARRIAVREGNALIVDGQNVDDKSDYRPGAAAGREMGVRSPLQEAG
                     LRKDEIRAYSRKLGLPTWDKPAQACLATRIPFGVPIRRPALARVAAAEEDLRRLGFGR
                     VRVRDHGPVARIELDLDEIGRAMGEEMRRRINSRLKKRGYAFIALDLGGYRVGSLNQG
                     AKKVLKEKKKD"
BASE COUNT         4539 a         7577 c         7806 g         4342 t
ORIGIN      
        1 gcttggttcg atactttgac gccggcggcc aaggcctccc gggcctgggg gatattcatt
       61 accgccaggc cggatacggc cagaagccgc gtttcccgaa gacgttcccc gcccaggagg
      121 cgtcttttgc ggacgagcgt ttcaagcgcc ttggccgccg gcccggtgtt ggaccggccg
      181 agagccagaa gcatcgctcc gcgctcggtc gagcttcggg ccaggaagtt cttgcgcgag
      241 acgatttgaa tcatccgggc caaggcgttc tcgtcggatt ccaggcgggc ggcctgtccg
      301 gcctcgatcc ggatgcgctc gtccggatcg agaaggaatt ccagcgtcag cttccgggct
      361 agatcatggg acagcccgcc cagcgtccgg accgcctcca gccgtatttc gggatttccc
      421 gagcgggcga ggtcggccaa atggagaatc gcttttttat cgccccggtc tcccaggagg
      481 gtgatcagcg tttgaatcag ctccggcttt cggttccgga tctgggccgc ggtttcggaa
      541 aggcaatcgc ggaccataac ccggagatac tcgacgcatg cttcgcgcca aaggggatcg
      601 acggccgcct cgcacgcctc gatcgccgtc ggcagggtcc ggggcccgaa ccagacgaga
      661 aattcgaaca gcgggcgggg atcctcgatc cgttcgggac gggcgatgtc ccggatgatg
      721 tccaggtcgg tctcctgggt gatgagacgg attgtttgga ggagatcctc ggcccgggcc
      781 ggagaggaag ccaagagggc tttcctcaag tcggatatgg aattcagcag gaaaagggcg
      841 tttccgatgt ccgccttctt gatcaggatc ttgagatagg ttttcatgta ggtcaggatc
      901 tggcggaagg aatccaggcg gtcctcaaga tagagaacct cgaaaaccgt agcgatgaat
      961 tccttgtcca cggacacttc gtgctcggcg gagacgaggg attccaggtt cgaccgttcg
     1021 cccccgtcga tcgaacggaa aaactcggcg agaacggagc cgtccagggg attcgtccgg
     1081 ctgatttcca cgctccgggc gtgggccgcg gccttgtcct cggcgtccag ttggatccgc
     1141 ccggaaaaga gatgctcccg gtcgatctgg agctccttgg gccggtggac cgtcccggcg
     1201 gccacccggg cttctaaaaa gccgtccgcg gcgtcgtacc ggatgaattc gaagtcgcgc
     1261 tcccagagat cctcgatgat gtccccctcc tccgcccgtt cggcgtaatc ggccgtgatg
     1321 gccaggaagt cgaagagctc ctcccgggtc agcccgggga ggaaggtgag ggcccgcatc
     1381 ccgtcgttgt aatacaggta cggcaggctc ttgatcccgc tttcctcctg gaagaccggc
     1441 ccgccggcga aggtgaaggc gtcttccttg atctcgattt cgaactcgcc ttgctcctcc
     1501 aggaacgcgg acagccgctg gaagagattg tcgcggaatt tcgtgacgtt gacgtggccg
     1561 aacgggtaaa gccgcatcgc cgcgatcgta tgaacgaagc gcccgacgat gtctctgatt
     1621 tgccgaatgg tctcggcgtt gacgggaacg aatgtggata tcggtctttg ggccattccg
     1681 gcaaccgctc cccgcggaca tccctaagat aacattattt gtagaaaaat caatccattt
     1741 cctccggccg gccggaacgt cttttccggc cgccttgtgg accccccggc tttccggtat
     1801 catgaaaacg gcggcggcgg cggataacgg atcaaagaaa atgaatctcg aggagaagct
     1861 caagcggctc cggatggagc gggaggcccg gtcccgcggc cccgcgtcgt ccgtcgagaa
     1921 cacgtggtcc cgcctccagc gggaagccgg gctttcgacc aaggaaaagc tggaacagct
     1981 gattcggttg accgggggaa cagccggacc ccggccaaaa aaaagcgttc cgcgcgagcc
     2041 ggagccgagg ccgcctctgc aggtcttcga aaacgcctac ccgccggacg cccgctatgg
     2101 aaagctgaga atctccgacg ccctggccgt cggcggggag gttctccgtt atctcagccg
     2161 ggacgaggcg ttccgcgacc tcgatctttc gacgaccgtc tttctcgacc tcgagacgac
     2221 cggcctggcc gggggaacgg ggacgctgcc gtttctcgtc ggtctcggct atttccggga
     2281 gagggcgttc atcgtcgctc agtattttct cggcgacccg ggcgaggaag agcggatgct
     2341 cgaggagctg tcccggttgt tcgccgagag acgcttcaag tcggttctga cgtacaacgg
     2401 aaaagcgttt gacctgcccc tgctggagac gcgcttcatc ctccagcgga aatccttctc
     2461 cctggccggc ctgccccact tggattttct cttcgcggcc cggaccttgt ggaagcacaa
     2521 gcacgaaagc tgccgtttgt ttcacctggc ccagcaggtc atcgaggcgc cccgggacga
     2581 ggacattccc ggggccgaga tcccgtaccg gtatttcgat tacctgcgga ccggggattt
     2641 cgagcggatc gagcccatcc tttaccacaa ccaggaggac atcctctcgc tcctgggact
     2701 ggtcatctcc ggggcgcggc ttttttccga ggaggatctc gacggtgaaa cgggtccgga
     2761 tcccctcgat ctcgtcggca tcggcaaggt cttcgcccgg gccggggagg cggcccgctc
     2821 gacgcggctg ttcgaacggg ccctggtcgg acggcttccg tccgacgtcg ccgtttcggt
     2881 cgtccggcgg ctgacgtccc agctgaaaaa ggaagccgac tgggagaggg ccgtcgccct
     2941 gtggcgggac ggctcggcgg ccgatcatgt gttctgctac cgcgagctgg ccatttacta
     3001 tgagcacaaa gccaggaacc tcgccgaggc caagcggatc accgaagagg gtctggccct
     3061 ggccatggaa cggtcttcgg ccgagagggt cgattttgaa aaacggctgg cccgcttgaa
     3121 cgccaagctc cagaaaagcg ggccggtgaa aaagaagatc cggtctcacc gggggagcgg
     3181 agaagcgtcg taggaccgcg gccgcggccg gccggaggcg ccgggtcctt ccggcgctgt
     3241 cctttttcgc tcgtttctgg cataatatcg gccggccgcc cgacgagcct gtagctcagc
     3301 tggatagagc gtttggctac gaaccaaaag gccgggggtt cgaatccctc caggctcgtt
     3361 tctctttttc aattcccata tggatttatt gagcggcctg ctgtgccaag gaaaacgcgc
     3421 tgtgccacta tttgtgccgc cgagccggct tcccctcgag caatatcacg gcccgcttgg
     3481 ccgggccgtg gaaaacgggc gatgccggga tgggagccgc ctttaaagcc gatcgacggc
     3541 cggcatccct atagccaggg cagcttgacg ttgcgacggt attcttctca ggcggggccg
     3601 aacgtcttgg ccagatcgtc ctcctctcga ggcgaaaaca cccgggatcc ggcatagagg
     3661 gcgatcccgg cccgcgactc cgagccagga attgaagaga aaaccgagcc aaggcaggac
     3721 gaaaaacgcc gctcccgtgt acagcggatg tttgacgatc gcgtacgggc cggtcgtgat
     3781 cagctctttc cgggggactt tccgcaggat cagagcgacc gacctggccc agacgacaac
     3841 gccgggaacc aataaggcga tcgagacgat tttcaagacg ggcgacgggc cgccgacgct
     3901 gaagatcgac gggcgccaga cgttcagcgc cagcccgatg atgatcaccg gcaaaaccga
     3961 tctcgcgatt ttgtccccgc ttccgacaag agttttcagc ttcatgctta tccctccggc
     4021 cgtttgaaca ttcttttctt cccggaacga cgagttctct tgtattgtac ccttgggaat
     4081 tttttattcc aacggataaa ggggatgatc gccgatgagc tttttggcct ttcgtctgaa
     4141 aacatgagat aatttccgcg gtttccatgc ccttgatttc gccggttccc gttcagctcg
     4201 atccgggcga cgtcgtccgc gttcttcgct tggaccggcg cccgggagga aaaagggcgg
     4261 ccggcccgct cctggccgag gcggcctccc ttctccgccc gcgggctttc taccgggaag
     4321 cctttatcga gtcccgctcc gccgagggac tgacgatcgg gggccgcttt ttccgcagcc
     4381 gccttctccg cggccaggcc gggcgggccc tcaaggtgtt cgcctttgtt ctcacgatcg
     4441 gcccggagct ggaggaggcg gccgtccggg cgaaggatct ccttcaccgg tacgacctcg
     4501 aaacgctggc cgacatggcc gtcgaggcgg ccgcggccgc gcttggggaa cgcctcgccg
     4561 gcgcgtcggg acttggacgc ctggccgccc tgagtccggg aacggtggag gattggccgg
     4621 tcacggagca ggtcccgctc tttgcccttc tcggcgacgg ccccggccgg gccggcgtcc
     4681 ggctgacgaa ttcaatgatg atgatcccgc ggaagtcggt aagcggtctt ctttttccct
     4741 cgaaggaaac tttcttgtcc tgccgccttt gttcccggcg ctcctgtccg agccggaaag
     4801 cgccgcctga cccggaatta cggcgccgtc tcggcgatcg aaaagcctga acaagcctca
     4861 aaaccgtcct ttttctccct ccggggttgc aaaagaggcc gatttcgtgt ataaaatcct
     4921 ttaatttttc cgatttttcc cctgtttcga ggaggcgacc atgaaaaagg aatttaccgc
     4981 tgtttcgatc ccgacgtccc tctacaaaga aatcgaggag gctatccgcg gaaccgccga
     5041 tccgtcggtt cccaattacg tcgcccgagt cctccgcgaa tcgctgaaaa aaaccggttc
     5101 ccaggaggat gtgttcagcc cggaagatga agaaaaggtc aaggatcgcc tgaaagccct
     5161 cggttatatc gattgatggc cggggatgcg tctcccggga cagccgaagc ggcatccccg
     5221 gagaaataca aacgaggagc aatttgatga ttcaacccca tggcggacag ttgatcgacc
     5281 gcgtcctgaa gggcgcggcc ctggacgaag cgctcaagaa ggcggcgtct ctcccgtcga
     5341 tccctcttga cgccgagcag accagcgatg tcgagaatat cgccacgggc gtcttcagcc
     5401 ccctggaagg ctttttgggg gaaaaggatt ttcggagcgt cctggacaaa atgcggttgg
     5461 ccaacggcct ggcgtggacg attcccgtga ttctggccgt tgaccggaaa acggccgacg
     5521 gcctggccgc gggggcggac gtcgccctcc tgaatcccgc cggcgagccg gcggccatcc
     5581 ttcacttggc cgagaagtac ggcttcgaca aggccgagac cgcccggaag gtcttcggca
     5641 cggaagacac cgcccacccg ggcgtggcca agatcatgag cctgcccgac gtcctgctgg
     5701 ggggatcgat cgacctgatc cgggaaacgc cgacgccctt ccagaagtac aagctcaccc
     5761 cgcgggaaac ccgccggctg ttccaggaca agggctggtc gacggtcgtc ggctttcaga
     5821 cccggaatac cccccatatc ggccacgagt acgtccagaa atcggccttg accctggtgg
     5881 acggcctgtt catcaatccg gtgatcggga agaagaagaa aggagactat cgcgacgatg
     5941 tcatcctctc gtcttacgag gagctgatca agaactacta cgtccccgaa cggaccacca
     6001 tggccatctt gatgatggaa atgcgctacg ccggcccccg ggaggccatt ttccacgcga
     6061 tcatccgcaa gaatttcggc tgctcccaca tcatcatcgg ccgggaccac gccggcgtcg
     6121 gcagctacta cgcgccgttc gcggcccagg aaatcttcgc cgacttcccc gacctggaaa
     6181 tcacgccgct gttcttccgg tcgtttttct actgcaaaaa gtgcggcgcg gtcgtcagcg
     6241 acaagatctg cccccatgga aaggatcacc agatccagtt ccgggggacg gagattcggg
     6301 acatgctggt gcgcggcgaa tatcctccgg ccgagctcgt ccgtccggaa gtcgccaaga
     6361 tcatcatgga cgagaaaaac ccgttcaacg actgagcggg ggataaagga taagctctca
     6421 tgcgcaaagc tttgatcatc ggcctcgact cggccccggc ggagatcgtc ttcgaccgcc
     6481 ggaaggaatt ccccaacctc aaccgcctcc tggaaaacgg atcctacggg accatgcgca
     6541 gctcggaccc gccgatcacg atcccggctt ggatggtcat gagcaccggc aaggacgccg
     6601 gacgcctcgg gctctacggc ttccgccacc ggaccggccc ggcgtacgac aaaatgtgga
     6661 tcgccaccgc ccagtcgatc aacgagccgg cgatctggga catcatcggc caggcgggag
     6721 gacagagcgt cctggtcagc gtccccccca gctatccgcc ccggccgctg gccggcaacc
     6781 ggatcggctg tttcatcacc ccgggcgcgg acaaggacta cacgtatccc aaggagctca
     6841 aggcggaaat cgaggaaaaa ttcgggccct acctgttcga cgtccccttc cggatcgaga
     6901 accgggccga gatcctcaag gaaatctacc ggatgaccga gaagcggttc gaggtcatcc
     6961 agtacctcat gcgggccaag ccttggaacc tgttcatgtt cgtggaaatc ggggtggacc
     7021 gggtccagca cgccttctgg aagttcttcg acaagaccca tcatctttat gaagccggca
     7081 acgagttcga ggccgtcatc gtcgattact acaagttcgt cgacgagaag atcggcgcgc
     7141 ttctcaagga agccggcgac gacaccgtcg tcctggcggt ttccgaccac ggagccaagc
     7201 ggatgaaggg ggccttctgc atcaatgaat ggctcatcgc cgagggcgac ctggtcctgg
     7261 gcgcggtccc ggcccgcggc gccagcatcg aaaagacgcc catcgattgg gccaaaacga
     7321 aggcttgggg ctggggcgga tactatgccc gggtgttcct caatgtcgag ggccgagagc
     7381 cccaggggat catcaagccg gccgattacg agaaggagcg ggaggccctg gccgagcgcc
     7441 tgcggggaat ccgcgggccg aacggcgaga aatgggccac ccaggtcatc aagcccaacg
     7501 agtactacga cgacgcccgg ggcgactatc ccgatctcat ggtttatttc gacgacctct
     7561 actggcgttc ggccggcacc ctgggctggg ggacgatgta tctggccgaa aacgacaccg
     7621 gcccggacga cgccgtccat gcccagcacg gcatgtacat tttctatgac ccgcaggcga
     7681 aggtttcgac ccgcaaggac atcgacatca ccgacatcgc cccgaccgtt ctcaaggccc
     7741 tgggcctgcc ggtcccggcg gacatgaagg gccggtccct tcgttgattt tataaggagt
     7801 aacgtttatt atgtgcgatc atcaacatgc gaaaggcgtg acgctgtggt tcaccgggct
     7861 gccctgttcg gggaaatcgg ccgtggccga ccgggccgcc gagatcctga aaaacggcgg
     7921 ctacaacgtc gagcgcctcg atggcgacat cgtccggcag gacttgaccc gcgacctcgg
     7981 cttctccaag gaggaccggg acgaaaacat ccgccgggtg acgttcgtgg ccaagctcct
     8041 gacccgcaac aacgtcattg tcctgacgtc gttcatctcg ccttacaaag ccatgcgcga
     8101 gcacgcccgc aaggaaatcg gctcgttcat cgaagtctac gtcaacacgc ccctggagac
     8161 ctgcatccag cgcgacgtta aaggcatgta caagaaagcc atggccgggc agatcaagga
     8221 attcaccggg atttccgatc cgtacgaggt tccggaaaat ccggagatga tcctggaatg
     8281 cggcgccgag ccgctcgagg agagcgcccg gaaggtcatt cagaagctga aggacctggg
     8341 cttcgttcgc tgaccggtcg cgggaccggc gcgcagcggg agggggacat gagccgctat
     8401 ctcgtgaccg gaggcgccgg attcatcggc tcccacctgg tcgagacgct tctcgaccgg
     8461 ggggaggagg tccgcgtcct ggataatttt ctcacgggga agcgggaaaa cctcgcttcc
     8521 ttcggcggcc gtctcgaagt ggtggagggc gatctccgcg atcccgacgc ctgccggcgg
     8581 gccgtcgacg gcgtcgactt cgtcctccac gaggcggctc tgccctccgt cccccggtcc
     8641 gtggccgatc cgttcacgac cgatgagatc aacgtccggg ggacgctgaa cctcctttgg
     8701 gcggccgccc gggccaaggt ccggcgcctc gtcttcgcct cttcgtcgtc ggtatacggc
     8761 gacgcccccg gcctgcccaa gcgggaaggg gaggagggaa agccgctgtc tccctacgcc
     8821 gcgagcaaat gggcggggga gaagtacctc caggtcttcg ccaagacctt cgacctggaa
     8881 acggtctcgc tccgctattt caacgtgttc ggcccgcgcc aggatccggc ctcccaatac
     8941 gcggccgcgg tcccgctgtt catcacccgg attctccggg gcgaggcgcc gacggttttc
     9001 ggcgacggcg aacaatcgcg ggatttcacc tatgtcgcca acgtggtcga agccaacctc
     9061 ctggcctgca cggccggggg agcggccggc ggggtgttca acgtcgcctg cgccgatcgg
     9121 atcacggtca acgtcctgat cgccaagatc aacgagatcc tgggaaagtc tatcgccccg
     9181 gtttacgccg cgccgcggcc gggggacatc cgtcactcct tcgccgatat ttcggccgcg
     9241 gaacgggagc tcggctaccg tccggtcgtc ggctttgagg agggattgcg gcgcacaatc
     9301 gcctggtgcg ggcaaagggg gaaccatcga tgaatacgca aagccgcgcg ctgatgagga
     9361 aaatcaagac gaaggaggcc gccatcggag tggtcggcct cggctacgtc gggcttccgc
     9421 tggtcaaagc cttcctgaac aagggattca aggtgaccgg gttcgacgtc gaccggaaga
     9481 aagtccgcct cctgaacaag gggcaaagct acatcaagca tgtcacggcg accgaattga
     9541 agaaatacct ggccgtccgg cggttcgagg cgacggacga tttctcccgc ctgaaggaaa
     9601 tggacgcgat cctgatctgc gttcccacgc ccctggacgc ccatcgcaat cccgacctgt
     9661 ccttcgtctt gggcacgacg gagacgattg ccgcctatct ccggaagggc cagctcgtca
     9721 tcctcgaaag cacgacctat cccggaacca ccgacgagga aatgctcccg atcctggagc
     9781 ggaaggggct gaagggcgga aaagatttct tcctggcctt ctcgcccgag cgggaaaatc
     9841 ccggggatcc ggtcttttca gccgaaaaca cgcccaaggt cgtcggcggg ctgacgcccg
     9901 actgcctggc cttggccgac gccctctatt cgcaggtcgt ggccaaaacc gtccccgtct
     9961 cgtccacccg ggtggccgaa tcggccaagc tcctcgaaaa tatcttccgc tccgtcaaca
    10021 tcgccctggt caacgaattg aagctgattt tcgaacggat ggacatcgat gtctgggaag
    10081 tgatccgggc ggcctcgtcc aagcccttcg gattcatgcc cttctatccc ggccccggcc
    10141 tgggcgggca ttgcatcccg atcgatccct tttacctgac ctggaaagcc cgcgaggtcg
    10201 actaccagac gaagttcatc gagctggccg gcgagatcaa cacgaaaatg ccctactacg
    10261 tcgtcaacaa gacgatggaa gccctggccg cccggggcaa gtccctcaag ggctcgcgga
    10321 tcctcgtcct gggcatcgcc tacaagaaaa atatcgatga tcagcgggag tcgccgtccc
    10381 tcaagatcat cagcatcctc cgggccaagg acgtcagggt cgaatacaac gatccctacg
    10441 tcccggaatc ttcgggccac cgggaatatc cgggcttaaa gcttaaatcc gtgcccttga
    10501 ccgcggcccg gctccgggcc gtcgacgcgg tgatcatcgc caccgatcat tcctgctacg
    10561 actacgattt catcgtgaag aacgcccggc tcgtgatcga cacgcggaac gcggtcaaga
    10621 caccgcggaa aaacgtcatc aaagcctgac ggactcgggg cggattcccg aaaaaacggc
    10681 gatccggccg ttcgtcaagc cgagagtctt tttacgttcc gtccctgagc cgataaacgg
    10741 tgaatccgct cttttgaagc gcgggcgcgt attcgtcctc gatttttctc cggagtcttt
    10801 ccgggaattt cttcacgaca tacaacgcgg agttctggat gaggaggaat cgcggccgga
    10861 gagccccaag gtcggcctcc aggagggacg gcttgagcgg tttgagatga aaatccaatc
    10921 tccgggcttc ggcccaagtc gatgtataat aaatcagcga tccttccgcg tacaacagga
    10981 cgtcccggtc atcgattata ttcttttggg ccagctttgc ctggatcgcg tgaagcacga
    11041 attggattct tttccggctg ctcccgattc ccgaggggga gacgatataa gcgtttttat
    11101 cccgggccag ggtcaatcgg tcattgacgt ttttcacggc cagcccgtag acggagaagg
    11161 cgacggggcc ggacagaagg agggcgagag ccccggccgc cgccgcccgg cgaagggccg
    11221 gccgccggat cttggcccgg acaagcgccc ggaggcggga atccgccagg gaaagcagat
    11281 aggccgccag gagatagaga gccgtggcgc cctggagaag ccgctcgaag atcgcataga
    11341 cgaacgtctc gctggagaac gaaaggacgg cgatcacgag gatcgcgagc acggtctgat
    11401 tttcccgcgt gaggcgccgt tcccggaaaa cacgggtcag caacagggcg gccgtcgccg
    11461 ggagaatgag aaggaaaatc catagaaaaa tcttttcccc gtccagcgga gactgcaggg
    11521 ttttcccgag ggaggggaaa tgatgggaat aatgagcaac cacccgaaga tgatcggccg
    11581 gccggccgat gaacatttcg taaagcagct ttatcccgtg ggacttcagg aaataaaacg
    11641 cggccagggg agaaaagacg ccggccatcg tcaggaaata gcgactctgg tgtttgagat
    11701 ttcccttgag gaacgcgggc gcggagaagc ggccggactc cgtttcgggg agagcctcat
    11761 aaacctgcca agcgatcaaa agggccgcgg ccgcccaggc gtacagggcg atgtcctggc
    11821 ggaatccgag ggtgaacccg gcgacgaccg cggaggaaag gagggctttt ttccgcagcg
    11881 gagcggcggc gagtttctcc aggaagatca agttgagaag ggtgacaaag ggaaggagcg
    11941 tcttccaggg aaagcaggcc atgacgccga tcatggcgac cggcaggaag gtccatcctt
    12001 tggggaggag ttttgtagcg aacaggtaca tgaggacgtt gaccagggag cgaaggacga
    12061 cgaagaacag ccgctccacg gccacggacg gagaaaacac cgtgaacaaa agagccagaa
    12121 gaaagtatct cccgggcggg taggtgaagc cgacgaaatc gacgagggga agctctccgt
    12181 tgagagtgcc cacggatttc ataaccaggg cggcttcatc cccgtccagg atgatgctct
    12241 tattcaaggt caagttgaaa atcaggcttc cggcgaagag gatcagcgga acgacccatt
    12301 ttcttttgct agttcgcatg tcgggactct gcggacggcg tttcagacgg ccaggcgcgc
    12361 cggggatccc tggtcgtttt cattccgcgg gattcgggct cggcgatcga atcgcgtcgg
    12421 cgtctccctc ccgaaaaccc gccgatcgcg atcccccctc ataaaaaaat gataggctgg
    12481 gcgcgacgcg gtgtcaagga agacccggaa gaagcggttg acgattcgtt ctctgcggaa
    12541 tccggcccgg gagcggcccc ggggcaaaaa aaccggccgg actattccgc cttgggcgga
    12601 tggggtttcc ggcgccgccg gcggcgggga cggcgcttcc cttcgggtct tggctccggc
    12661 tctccggacg atggccgcgg ccggccgaaa agcttttccg gaaacgcgcc cggctccggc
    12721 ggtcggccgt cctcgatcac tccgtagatc cggacggcgg actcgaataa agcttggcgg
    12781 cggagggacc accggggccg gccggtccgc aagacattca tgatttggga gacgagcgtc
    12841 aggatatggg cgacgtccgt ccggaccttc cgggggaata cggcctgcag gccggcgctt
    12901 tcgaaggctt tttccaggac ggcccggata tcgccgtcct taccggcgaa gaatgattcg
    12961 gcccagggcc agaaaaacag ggcataggcc tcttcccggc tccagacgac gccttccttc
    13021 tgaagccggt ctttcgcttc cagaagcgcg cggagacgct cgaaaagcgc gtcgtcgctt
    13081 tcataatctt ccccgaagct gccgatgatg tggcggagaa gtccatgggc ccggagggcg
    13141 tccacgaccg gccgggtctt ttcgaccagc tccttgttga gttcctcata cagccgggcg
    13201 ccggagcatt tcccgagcag gggagcgaag accgggatcg cccgggccgt ctcctcctcg
    13261 atgtcgaagc cgagacgggc ggcgtaacgg acgacccgcc agatgcggac cgcgtcctcg
    13321 gcgaaccggg cggccggatc gccgatgatc cggacgcgct tgagggccag gtcttccagg
    13381 ccgccggcgt aatcgatgac ttctcccgtc cgggcgtcgt agtacagggc gttgatcgcc
    13441 acgtcgcgcc ggaaagcgtc gtcccgcgga gtcccgtaga gcagctcggg cgggattttt
    13501 cccggcgggg ccgcctgttc ctccgccgaa agatcgcgcc gaaaggtggc gacttcgatg
    13561 attttgccgc cgggaaaatg aacgtggcac aggcggaagc gccggccgat gatgaagacg
    13621 ttggcgaaac gctttttaat ctgcccgggg cgggcgtccg tgacgacgtc gaaatccttg
    13681 ggcgtccttc cgagaaggag gtcccggacc gctccgccgc agaggtaggc gatgaagccc
    13741 attctctgaa gacgggcgag aatcctcagg gcgtcgggat cgacatccga ccgggaaagg
    13801 cagtgctcgg agggggaaag gatgcggatt gtgaccgtca tgagcgggag cttccttgag
    13861 aggttttttt tgcccggagg acgagggtgt gggaggcgaa atcggtttcc aggaccagca
    13921 agacatcctc gcctaaacgt tcgatggaga agggaacagg gcggccgtcc cggcggacgt
    13981 cgagctccgc ctccgacagc gcccggggca ggaggagacg gatccatccg ggccgggaag
    14041 aggggctttc atatcggaag acgacccgct gttcggccgg attccaggaa tattcgtagg
    14101 ccgccgcgct tcccgaggcc ggcaggcgga aaaagacttt ggctccgtcc tcggccaggc
    14161 gcggttcgag gcggcagccg tcccgcgtca aacggacacc gaaatatcct tcggccacgg
    14221 ccgaggccaa gcttccggcg ctgccggcga agacggggct gccgcgtccg cttccgtccg
    14281 gcgcgttcca ttcgtggagg cctccggcgg cgatgttctt ccgggcgatc tccaagagct
    14341 tggcccgggc ggccccggaa tatccatgat cgaacatcgc ccggacgagc cttccggcga
    14401 accagtccca caaaccgccg ttttggtatt cgtagggatc gtcgaccatg gggtgtttga
    14461 aggttccttt cgggtagggg ggaagcaggg ccgccccgat cgtcggcatc cggaatgctt
    14521 cctggcgggc caaggcggcc tggatgatgc ggacggcttt ctccgggccg gccaagccgg
    14581 aaacgacggc ctcggcgttc ccccccatgg cgaacatgtc gtcctcatcg aagggatgga
    14641 ccgggccgtc gagatggacg tggacgcggt aaaatccgcg gctttcctgc cagagatagc
    14701 ggtcggcggc ttcccggacg ctttcggccc gccgcgtcca gaaatcggcc cgctcaaccc
    14761 ggccggtgtt ccggagcatt ccggcgagcg cccgggccgc gccgtaaaac atgctttggt
    14821 cgtagatgtc acatgtccag cgggtgcttt ggccggcgta gatcgccgtt tgatcggctt
    14881 cctccattcc gaggtccccc cagtcggccg tgtgggcgcc cgtgaccaag ccgcgtttca
    14941 cgtcgaaccg tcgttcgaga acgaaggaga gggccaggtc gagccggtcg aggaccggcc
    15001 ggccggcgat ctttttctcc agccacccgg gaccgtggat ctcgacggcc cgggcggcgg
    15061 ccaggatcgc ggaggcttcc tggtccgttt ccgtgatgtt tttatccgat cgcccgtggc
    15121 tgtcgaacca atcgggcaga ccgccgccgg tttcttgaaa ggcgagaaat tcttccagcc
    15181 aggagacgat ttcctccgcc ggagaataat accgggacgc ttggatggtc gtggccgcgt
    15241 cgcggatcca gatctgcgga tagaggcttc cggccgaaaa cgccgaaatc agtcccgact
    15301 tgccggggaa ggacatccgg ttggcttcga gcgtcgcccg gatgagatgc cagagggagc
    15361 ggatttcggg aacgctcgtc cggaacgtcg tcgtcgtctg acccggggcg gcggcgggaa
    15421 aggcgaaggc ggcccacaag gccagggccg tcgccgggag aatcggccga ggccggaaaa
    15481 acctcaacga gggattcagc atatccgggg aagaagctcg gaggtcaagg gctcaagcag
    15541 gcgcagaccg ccgctttcgg tccggagaag aagctcgccg ggccggccgg gggctttgtc
    15601 cgcccggccg acgatcgcgg cccggcggcc ctggcgatgg ccgcggaggg tggagaggac
    15661 gccctccgcc cggttctcgg ggacgatcat gaccgcccgg ccttcgcagg ccaggtagag
    15721 cggatcgatc ccgagaagtc cggccgccga tttggcggcc gtcgacaagg ggatggcttt
    15781 ttcctcgagc aggatcggac agggcgattg ttcggccagt tccgagagaa cggtcgccaa
    15841 tcctccccgg gtgatgtcgc gcatccagcg gactccccgg gaccaaagcg gcagaaggaa
    15901 ggtcaggggg gcgcagtcgc ttttcaccgg gttttcgaag ccgaattcgc cccgggcggc
    15961 caggacggcc aagctgtgct ctccgagcgt tccggtgacg atgacccggt cgcccggccg
    16021 gatggccccg ggccgggcgc ggccgatgat ccggccgatc ccggaagtgt tgacaaagat
    16081 cttgtcgcct tgtccccggc ggacgacctt ggtgtctccg gtgacgattt caacgcccgc
    16141 ggcgtcagcc gttttccgga tagagcgaag aatccgctcc aaggaggccg tttccaggcc
    16201 ttcctccagg atcagggcca gggagaggaa ccggggcttg gccccggaga cgacgaggtc
    16261 gttgaccgtg ccgttgacgg ccagggttcc gatgtcccct cccgggaaga agatcgggtc
    16321 gacgacgtag ctgtcggtcg tgaaggcgat gccgcccggg agggaggcgg cgtccggaag
    16381 accggccagg cgggcgttgg gaaaggtcgg cagaatcgac cgggcgagga aatcgcgcat
    16441 cgttttcccg ccgccgccca gctcgaggct gattttatcc atgggatttt ctgctccttc
    16501 tttgagccgg ggacgatccc cggtatttat gatgaacgta acaagccccc tcgaaagaga
    16561 ccatgcaggg cccgcggggc gattcgggcc ggcaggagcg gccgaacaac cggcattgag
    16621 gcggcgaaat aacgcctcgc aggatctcgc cgcaccggca gccgggggag tcggacgcgt
    16681 cctcggccgg acgaagggca tacttgacgg ccgcgtcgac ggccgcgtgg gccggtttga
    16741 agcgcagccc gctcgccggg atgcgtccca atccccgcca gagggcgtca taaggctcga
    16801 ggaaccggtt catcacctcc cgggcggcgg gatttccttc cggccggacg acccggggat
    16861 aggcattgac gaccgcgggt ttcttcgccc ggatctgctc cacgacggaa cggacgccga
    16921 ggaggatgtc gcccggctcg aagccggcga ccgcccccgg cagcccgaac tcccgggcga
    16981 cgaaggcata gggttccagg ccgatgatcg cgctgacgtg gccggggtag agaaatccgc
    17041 tgatgcggat ctcgcccgag gaaacgatcg cccgcagggg aggggggatg gtccaaaagg
    17101 ccgtcaaaac ggcgatgttt ttcaatcctt ccgcttgggc ccgcccggcg gtcaggacga
    17161 tggccgggat cgtcgtttcg aaacccgccc cgaaaaaaac gacgtccttg accggattca
    17221 agcgggccag ggtcagcgct tcttccggag aatagacgat tttaacggcc gagcccggcg
    17281 ccgggacggc cgcctggagg gatccgagcc gggtgggaac ccgggtcatg tcgccgaacg
    17341 tcgccaggat gaggttttcc cgttccgtca ccagccggat ggccgcttcg tggaagccgt
    17401 ccggggtgat gcagacgggg cagcccggtc cggaaatcat ctcgacgccg gcctgggcga
    17461 gcaggtcctt gagcccatga cgatgaatcg tcatggtgtg cgtgccgcag acttccatca
    17521 accggaccgg cggggaggac gacgcggccg cggcggcgat ttcggagagc aggccggcga
    17581 cgacccggcg gtcgcggaac aagtcggacg ccacctcagg atctcccggc atcctggaca
    17641 tcacggagca gggccagcgt ttcccgggct tccgcttcat ccaggcggga gatggcgaat
    17701 ccggcatgaa cgatgaccca gtctccaacc cggacgtccc ggatcagggc gaagttcgcc
    17761 ccgattttcg tccccagata atcgactttt ccggcatcgc ccgaggcgtc gatttcgacg
    17821 acccggacgg gaatcgctag gcacatgaca acctcttgat agggcatagg atatcaaaac
    17881 tcgccctccg aatccatgga cggccggccg gccatccgct atactatgcg catgttaaaa
    17941 ggcttggacg gatggcttga ggacggcagc gaggaagccg gcctggctct ccgcctggac
    18001 gccgcccgga ttccccgtca cgtggccgtg atcatggacg gcaacggccg gtgggccgcg
    18061 gaacggggat tgccacgggt cgaaggccac aaggccgggg cgaaatccgt ccgggaaatc
    18121 atcgagacgg gcgcccgcgc cggcctggac gttctctcgc tgttcgcctt ttccaccgag
    18181 aactggaaac gccccccccg ggaagtccaa accttgatgc gtcttctcga ggagaccctg
    18241 gggaaagaag acggacttct catggagcac ggcctgcggc tgcgcgtcct gggggcgaaa
    18301 gacaagcttc ccggagggct ccgccgcgag ctcgagcggg tcgagacgat gacccgcggc
    18361 aattcccgga tgatcatcgg tttggccttg aattacggcg gccgggcgga aatcgtcgag
    18421 gccgtccggc ggattttggc cgaggggcgg ctcgcggccg gcgaccttga cgaaaacgtt
    18481 ttcagccggt acttggacac ggccgggctg cccgatcccg acctgctgat ccggacgagc
    18541 ggcgaacgcc ggatctccaa cttcctcctc tggcagatcg cctacgccga aatctggatc
    18601 acgccggttt tctggcccga tttccggaga aaacaccttc tccaagcctt ggtcgattac
    18661 caaggccgga accggcgctt cggggccgcg gagccgtcgg catgaatatc gtcaagcggt
    18721 tttggacggc cctcgtcctg ttgaccgccc tgttcctggt cctcgaatac ggaaacaagt
    18781 gggttctgtt cgccgctttg caggccgtcg tcatcgcgtc cctgatcgag ttctacaacc
    18841 tggcccggcg gcgccgcctt tatccggcgg ccgttcccgg gatcctcttc gcccttctct
    18901 tgggggcgtc gttcctcatc gaagggctga cgctggagct ggctctcttc gccggtctcc
    18961 tgggcctcgg cgtcttctat gtcgtcgtct cccggacgat cgagacggcc gtccgcacga
    19021 cggcctcgat cgccgtgacg gtcttcggcc ccctctacat cggcttcacc ctcaactttc
    19081 tgtacccgct tcgccagacc tacggttccg ccccgatcta cttcctcctg gccgtggttt
    19141 tcctggccga cacgggggcg ttttttgtcg ggaaggcggc cggacgccat ccgatgactc
    19201 cgatcgccag ccccaataaa acttgggaag gggcggccgg gggcctgatt ttcgccggtt
    19261 tgaccgcctg ggccgcaagg gcggttttcc ttccggagat cgggccgggc cgggcgatcc
    19321 tcaccggact ggccgtctcg atcgcggccc aggccggaga tcccctcgaa tcccttttca
    19381 agagggccgc gggcgtgaag gattcctcca acgcgcttcc ggggcacggc ggcttcctgg
    19441 acagggtcga cagcctcttg ttcgccgtcc ccctcttcta ttttatcgtc cggtacatct
    19501 ggaagcccct cggatgagaa aaatcgccgt tctcggctcg actggcacga tcggccggaa
    19561 cacgctcgac atcgtccgcc gccatcgccg tctttttaag gtgacggcct tggccgccgg
    19621 ctccaatcgg gacttgctcc tctcccagat cgtggagttc cgcccgcttc tcgtctccgt
    19681 cgggacggac gcggacgccg ccgccctgcg ccgggagctc gggcggcggg ggccggatat
    19741 cctgtccgga gcggaaggag cctcggccgc ggccgccgaa acgagcgccg accttgtcgt
    19801 ttcggccatc gccggattca acggcctgcg gccgaccctg gccgcggtcc gggccggaaa
    19861 gacggtcgcc ttggccaaca aggaatccct tgtcgcggcc ggcggactgc tccgccggga
    19921 ggccaagaac agcggagcgg tcttcattcc ggtcgattcc gagcacagcg ggatttttca
    19981 atgtttggcc ggcatcgatc gcggggacat ccggagcgtc gttctgaccg cctcgggcgg
    20041 cccgtttctc cgtatccccc tgcgggagtt gcgccggaag acgccggaag aagccttggc
    20101 ccatccccgc tggacgatgg gccgcaaggt caccatcgac tcggccacga tgatgaacaa
    20161 gggcttggag ctgatcgaag cccggtggct tttcgacctg gccccggaca tgatccgggt
    20221 tctcattcat ccccagagca tcgtccacgc cttggtcgag ttgcgggacg ggtcggtcct
    20281 ggcccagctc agccggacgg atatgcgcat ccccattcaa tacgccttaa cctatccccg
    20341 gcgccgggat ggattccttc cgccgctgga tctggccgct tccgggccgc tggaattttc
    20401 gtctccggac gagcgccggt ttccgctcct taaaacggcc cggcgggtga tgggagagaa
    20461 ggacagcgcc gcggtggcga tgaacgcggc taatgaaatc gccgtcgagg ccttccttgg
    20521 ggcccgtctc cggttcaaca gcatcgccga aacggtggag gaggccttgg ccggcctgcg
    20581 gccgcgccga ttggattccc ttgacgacat catcgaagaa gaccgccgaa tccgggagcg
    20641 gacgaggcgg cggatcgaaa aaaggatatg acatgggcac gattttcggc acgattctcg
    20701 cgtttttaat cgtttttggc attctggttt ttattcacga attcggccac tttttcgcgg
    20761 ccaagctggt cggcgtcagg gtggaagtct tctccttcgg ctacggcaag cgcttgttcg
    20821 ggtttaaaaa aagcggcacg gattaccgga tcagcctctt gcccctgggc ggctacgtca
    20881 agatgctcgg cgagggcctg gctgagccgg gcaaaatcct ggagccggat gatttcatgg
    20941 ccaagaaccg tctccagcgg ttcctcgtct tcgtgatggg gccggtcatg agcattctcc
    21001 tggcggtcgt catcatggcc ggcctcaaca tggtcggcgt aacgacgccc cgctaccagg
    21061 acgaggcgcc ggtgatcggc tggatcgagc cgggctcgcc ggccgcccgg gcggacctga
    21121 agccggacga cctgatcctg gcggtcaacg gccggaaaac gccgacctgg aacgacgtgg
    21181 agatcgccgt cgggacaaag cccaaccgcc tcctgtcctt ggacatccgg cgggacggcc
    21241 gcgtcatgga ggttccgctg acgaccgaga gccggactaa atacgagatg ggatacgccg
    21301 gatttttcgg caagatcctg acccaggtcc agatggtttc cccgggctcc ccggccgaga
    21361 aaggggggct tcaagccggg gacgtcatcc tggcgatcaa cggcgagccg gtgtatttct
    21421 accgcttcgt cgagatcatc gagtcgaatc ccgagaagga atgcgcgttc cgggtcgacc
    21481 gcggcgggcg cgagatcgcc ctgagcatca ctccgcgccg ggagggcaag gtcgggaaaa
    21541 tcggggtgat gcagacggcc cagtccgtcc tcaagaaatt cgcctttttc ccggccatcg
    21601 gccagagcct caaggaaaac gcccgcctga cttttctggt catcgatttt ctcaagaacc
    21661 tgttcaccgg ccaagcctcg gccaaacagc tctccggtcc cctggaaatc gccaatttct
    21721 cctacgccgc cctgaaaatg ggatttctct ccctgatgag ctggatcgcc ctcatcagcc
    21781 ttcaactggg aatcctgaac cttttcccga tcccggtctt cgacggcgga cagattttcg
    21841 tcctggccat cgagggaatt ctccggcggg acctgacgcc caaggcccgg cagatctgga
    21901 tgcagatcgg gttcgccgtc ttcatcctcc tgatcgggtt cgtcattttg aacgacatcg
    21961 ccaagcgcct gcccaacggc tggaggagct tgttgccgtt ttaaattcaa ggattgtcat
    22021 cggccggcat attcagggtc ctgccgcctc gcagccccgt cgtcctcgct ccacccatcc
    22081 cccggtggga cccttccgct gcgggcgacg gggcgcccag ctcggcgtca ccctgaatcg
    22141 gccggccgat gaagcctggt cctgagccca tttaaacggc cacaagcccc ggacgaaaaa
    22201 aagagcggat tcttcgaaaa gcctgaaccg gacatgtccg ctcaagccgg gtctcgggga
    22261 agggaagaaa aagagccgcg gcggcggcat tgctggtata atggcctcca tgaaggaaca
    22321 aggtcctccc ttattccccc gccgccgaac ggttcaaatc cggatcggaa acgtagctgt
    22381 cgggggagga tctccggtca gcgttcaatc catgaccaag accgacaccc gcgacgtccg
    22441 ggcgacggcc gctgaaatcc gccgcctcgc cagggaggga tgcgagatcg tccggctggc
    22501 gatccccgac gcggaggccg cccgggcctt cgcccgcctc aggaaagcgt cgccggttcc
    22561 gctgatcgcc gatatccatt tcgaccaccg cctggccgtg gcttgcctgg aagcgggggc
    22621 cgacggcctc cggctcaatc cggggacgat cggaacgcgc gacaaggttc gagaggtcgt
    22681 ccgggcggcc aaggagcggt ccgtcccgat ccgcatcggg gtcaacgccg gctcgatcga
    22741 aaaggatctt cgatccgccc ggggagggac gacggccaag gccatggcgg aaagcgctct
    22801 ccggcacatc cggatcctgg aagacctcga ttttcggctg atcaaggttt cgctcaaggc
    22861 ctccgacctg acgcggacgc tccaggccta ccggctcctg gcggagcgca cggattatcc
    22921 atttcacgcc gggatcactg aggcgggcac cctgctgccc ggggcggtga aatcctcggc
    22981 cgggttggct ttgctgctgg ccgagggcct ggccgacacg atccgcgtct ccctgactgc
    23041 tccggcggtc gaggaggtcc gggtcgccta ccggatcctg gccgcgatgg gtctccgggc
    23101 gcgcggcctc gacatcgtct cctgcccgac ctgcggccga tgcgaagtcg accttgcctc
    23161 gatcgtccga cagattcatc gccgtttggc cggcgttccg gagccgctga ccgtggccgt
    23221 catgggatgc gccgtcaacg ggcccgggga agcgagggaa gcggatgccg gcgtcgcctg
    23281 tggtcggggt tcgggcgctc tgttcatcaa gggagagatc ctccggcggg tgcccgagaa
    23341 ggaaatcgcc gacgccctgg tccgcgaagt cgtccggatc gccgccggcc ggaaagacgg
    23401 cgatgagcgt cgctgaacat ccttccgttt cccgccggct cgaggagaag gtcggccggc
    23461 tgagggatat tctccaagga atggaccgcg tcctggttgc cttttccggc ggaaccgaca
    23521 gcgcgtttct cctgaaaatc gcggccgagg ttttagaccg gaacgtctcg gccgtgatcg
    23581 cctcctcccc ggtctttccc gcttcggaaa tcgccgacgc cgagcggttg gcccggaaat
    23641 tcaaggtccg gtacagaatc atccgccccg ccgttttcaa gaatccgcgg tttgtgaaaa
    23701 acacgcccct ccggtgctac gaatgcaaga aggatttgtg gcggtcggcc cggagaatcg
    23761 ccgtccggga agggaacgcc ctgatcgtcg acggtcaaaa cgtcgatgac aagagcgatt
    23821 accggccggg ggcggcggcc ggccgggaga tgggcgtgcg ttctccgctc caggaagcgg
    23881 gcctccgtaa ggacgagatc cgggcgtatt cccggaagct gggcctcccc acctgggaca
    23941 agccggccca agcctgcctg gccacgagga ttcccttcgg agttccgatc cgccgcccgg
    24001 ctctcgcccg cgtcgcggcc gccgaggagg atctccgccg gctgggattc gggcgcgtgc
    24061 gggtccggga tcacgggccg gtcgcccgga tcgaactgga tttggacgag atcggccggg
    24121 cgatggggga ggagatgcgc cgacggatca actcccgtct taaaaagagg gggtatgcct
    24181 tcatcgccct cgacctggga ggatacaggg taggcagcct gaaccaagga gcgaagaagg
    24241 ttttaaaaga aaagaaaaaa gacc
//
LOCUS       DAOOXH010000031        24175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_1904_length_24175_cov_6.810945, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000031 DAOOXH010000000
VERSION     DAOOXH010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 24175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24175
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1904_length_24175_cov_6.810945"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="PKV64_05330"
     CDS             complement(<1..914)
                     /locus_tag="PKV64_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPN16538.1"
                     /translation="MSRGLSLPGSILGALILKPELLELSDISGNDFPSGRLRETFSKI
                     AELWEDGRPKSIDPILLSEKIKGDGAAAFVAGLIDGNIRLDFESFRGRIFELRRKALR
                     IRALVELQKVLHEEEKTGEVDPESFARARESFRLLEELSGIAGAGDPARLLKTGEELQ
                     AFDIRIEWLIRGLVPADSITLLPGRGGIGKTWAALEIGKAVSRGQDFFNLPTLPRPVV
                     YVDFENPLPVLVERIKALEIPDVLFWTSAIDPRPPKIDSAGFELYKRLPAGALLIFDT
                     LRSAHNGDENDSREMALVLGRLKELRDSG"
     gene            complement(1439..2098)
                     /locus_tag="PKV64_05335"
     CDS             complement(1439..2098)
                     /locus_tag="PKV64_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16539.1"
                     /translation="MKEEIEKRIQESKRRLKAAEIRAELMKRNFRFRKEHKAAEKFPS
                     HSDEWKAFCDKWRISEAWNGRLEGLVECTYSLPSLHVSIPRARVSKKQVELKSWSFYP
                     DAGPWRGIGDALHHSIQDEDGYIYIRIHPWTKKKDIDYLWLEVRYAQKKVFGEIYGGR
                     QRRTFERDLCWYDLHRGKDFGKLSAGKIAMAWFLHRKEKIPRKTIESSIKNITLLIRE
                     L"
     gene            complement(2239..2311)
                     /locus_tag="PKV64_05340"
     tRNA            complement(2239..2311)
                     /locus_tag="PKV64_05340"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2276..2278),aa:Lys,seq:ctt)
     gene            complement(2468..3505)
                     /locus_tag="PKV64_05345"
     CDS             complement(2468..3505)
                     /locus_tag="PKV64_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HPN16540.1"
                     /translation="MASRKRLVAALTVFLLVFVIGVAGFKILGGPEWTILDAMYMTVI
                     TVATVGYGEVHDLSAHPAARAFGVVYVILCLGTIAFAVSSMTAFVVEGELRNLLGRHK
                     MEKAIAHLGNHYIVCGNDETAQTVVQELLQTKKPFLVVDPSAERMEKMKTLGEIAWIL
                     GDPSEDDVLRKAGIGRARGILCLLPSDEANLFVVVTAKSLNPAIRVVAKGIDVRSHDK
                     MKKAGADSVISPAFIGGMRMVSEMVRPATTTFLDLMLRERGRVLRFDEVTVPPGSPLA
                     GKTVGEADFESRTGALFVARRRGGGKDFDFNPGKNARIEAGDVLVFIAAPEMIAGLEK
                     IAGGPEKPVAA"
     gene            complement(3515..3591)
                     /locus_tag="PKV64_05350"
     tRNA            complement(3515..3591)
                     /locus_tag="PKV64_05350"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3555..3557),aa:Arg,seq:ccg)
     gene            3827..5644
                     /locus_tag="PKV64_05355"
     CDS             3827..5644
                     /locus_tag="PKV64_05355"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPN16541.1"
                     /translation="MKRHLVGAVAVIAAFLTLQAQMPPQPPLKLEDVQKALCLVDRPA
                     PVPEKSRAGFESITPRETTAMLAYLSSDWMEGRETGTSGYAQAADYAASLFKMWGLKP
                     AGDFPPAPRGPRRFGSTPPPPAAERSYFQNIAFKEISDVRSTLTVEVAKGPGVKTRAY
                     AGGVDFAIVPGPAVTISAPVVFAGYGISEPTVGFDELKGLDLKGKIVLILTEAPGKDN
                     PQSLFQADKALKEKYFPTFAGFEMMMRRGGPPRFSKVEAILARGAAAVVQVDNTSRDR
                     EIFRDLATPEKPSDDRPIIRKPRLRLQVAGDASAPMGGRAALTVTREIADAILEGTGQ
                     TVDEFKKKIETAFKPASCLVPGTKLTIATTARTSLVQSPNVLAVIEGSDPELKKECFV
                     IGAHFDHLGAWEDYVFNGADDNGSGSAGVLAIARAMAVNPVKPKRSVVFALWTGEEKG
                     LYGSRYYVAHPFFPLEKTVGYLNYDMISRSYEAAALALAVRRYSVPGAEEMVKKIRPD
                     RFASVFVTEGTDLVRLAKEMNAFVGLDLAFRESPLGSAGGGSDHAPFSQAKIPYVYFM
                     GAMTEDYHQTSDSIEKINPELFTKICRIGYLTAFARADQ"
     gene            5896..6633
                     /locus_tag="PKV64_05360"
     CDS             5896..6633
                     /locus_tag="PKV64_05360"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HPN16542.1"
                     /translation="MPEHPTAPKRPRIWEIDFLRGLSILLMVGYHLLYDLGAFVGLKR
                     FLGFSTDLFSPAWMIAQYFFAGLFVVLSGISSTLTRSNIRRGLRLLVVSLAVTAATYV
                     FDPPSTIWFGILQCLAVSMLLYGAAFEKARPAVSAAAGAAVFGLGFALPAVRRALVLR
                     SDWLLPLGLHSPLFMSYDYFPLIPWFGVFLIGAALGKTVYKSRRSLLPRPPRPIFINA
                     AGRYSLWIYLVHQPVIMGVLYLLGLVR"
     gene            complement(6751..8457)
                     /locus_tag="PKV64_05365"
     CDS             complement(6751..8457)
                     /locus_tag="PKV64_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPN16543.1"
                     /translation="MTETDDKKPRENFIRDIVREDRAAGRIEGRVHTRFPPEPNGYLH
                     IGHAKSICLNFGLAADFDGLCNLRFDDTNPETESFEYVASIKEDVRWLGFDWDDREYY
                     ASDYYEKLYEFAEILIRKGKAYVCDLGAEDVTATRGTLTRPGTDSPFRNRTVEENLDL
                     FRRMRAGEFPDGAKSLRAKIDMAHPNLLLRDPVMYRIRRAHHYRRGDAWCLYPTYDWA
                     HGQSDSIEGITHSICTLEFEVHRPLYDWFLKELGVFKPRQIEFARLNLSHTVLSKRKL
                     LLLVKENRVDGWDDPRMPTISGLRRRGYTPAAIRRFADLIGVAKVPSIVDMPLLESCV
                     REDLNKTSPRAMAVLRPLKIVLTNYPEDKVEMLECVNNPEDPSAGSRAVPFSRTLYIE
                     REDFLEDPPKKFFRLSPGREVRLRYAYFITCREVVKDPATGGVVEVRCTYDPATRGGD
                     SPDGRSPKATLHWVSAAHAVPAEVRLYDTLFTKRDPNELEEGEDWLANLNPKSLEAIS
                     EARLEPMLAEAAPGSRWQFERMGYFVADTKHSRPGAPVFNRTVTLRDVWTKLQKTGRA
                     DG"
     gene            complement(8454..9746)
                     /gene="gltX"
                     /locus_tag="PKV64_05370"
     CDS             complement(8454..9746)
                     /gene="gltX"
                     /locus_tag="PKV64_05370"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPN16544.1"
                     /translation="MKTPLTVRFAPSPTGFLHLGSARTALFNWLAARSSGGRFLLRVE
                     DTDKVRSEQRFLDEILEDLAWLGLDWDGEPMYQSRRFDLYREKAESLVASGRAYREGE
                     AILYRVDPGRKIAYDDLIHGRIEVETENIKDQVLIKSDGSPAYNFCCVVDDSELGITH
                     ILRGDDHISNTPKQILFYEAFGLVPPRFGHMPLILGPDGSKLSKRHGGVSVAEYKAEG
                     FLPEALANYLIFLGWTPSDGREILGLAEAARLFSIDRMNDVQAKFDLVKLKWMNGEYI
                     MAKETAALVPLIQDQLRRDGYDFDVSDGARWAAVVDLYKIRIKTLRELGPMIEFFFRE
                     DFPVDEEGRKKHLGAPEKKALLADLASRLEALPEFSAAGLEELYRGLAAERNIKVAEL
                     IHPSRMAVSGLTKGAGLFEIMAVLGRGTVVDRLRRNSL"
     gene            9883..10290
                     /locus_tag="PKV64_05375"
     CDS             9883..10290
                     /locus_tag="PKV64_05375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN16545.1"
                     /translation="MLIRKARPEDFPAIRKLAEALGLDYPGMEKALFWLAEKDGRVLG
                     IVSLLVHADSRELVSLGVDPAAREKGLGGRLIETAVAAAGADVYLATVIPGYFERHGF
                     ARTLRVPRGMAKDPAWCAGCDKSRCAVMVREKS"
     gene            10287..11162
                     /locus_tag="PKV64_05380"
     CDS             10287..11162
                     /locus_tag="PKV64_05380"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HPN16546.1"
                     /translation="MSLPAFPEFKPLALEDRSVVQGFFDRAAVEACEYSFGNNFIWRH
                     FEKTRWTILDGNLCLVFDCPGRPPYALAPVGGTAAAGAVRALLDVVPRLARVPAAFVQ
                     EHGADFLCEPERDEFDYVYAAADLADLAGKKYDGKRNRIRKFEREHQWRYVPLDNGHL
                     DAARRLFEEWFAERDDHSDMAQGQKTAISEALAHFRELGLLGGAIEVDGRFAALSIGE
                     PLTRDTAVIHIEIVSPCCEGLAQIMNREFVRHALKDFAFINREQDLGLPGLRRAKLSY
                     HPHHLVEKFRLERRP"
     gene            complement(11168..12529)
                     /locus_tag="PKV64_05385"
     CDS             complement(11168..12529)
                     /locus_tag="PKV64_05385"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HPN16547.1"
                     /translation="MIKTERLRVLNSRETKSGDYVLYWMQAAQRAEANPALQHAVRLA
                     NEQRRPCLAFFGLTERFPEANARHLAFMLEGLRETQLALADRGVRLVVRRIQPDRGAV
                     ELGRAASLVVTDRGYLRIQKSWRERAAAALACPLIQVETETVVPVEKASPKEEYSAAT
                     FRPKIRKILDSSLAVSPDRKPLRDSLGWKGDSIDLENIEDVLAGLKIDRSVGPSPFYR
                     GGTEEAKRRFRLFLRDRLDHFADLRNNPTVDFLSHISPYLHFGQISPVWAAAKAAASN
                     SRGREAFLEELVVRRELAMNFVHYNDRYDTFESLPAWARATLARHAADPRPVLYTRAE
                     LEAGKTHDPCWNAAQREMAVTGKMHGYMRMYWGKKILEWSASPEEAYATALYLNNKYE
                     IDGRDPSGFAGVAWCFGKHDRPWGERPIFGLVRYMNEAGLRRKFDADRYIRNVAELAA
                     RKD"
     gene            complement(12541..13041)
                     /locus_tag="PKV64_05390"
     CDS             complement(12541..13041)
                     /locus_tag="PKV64_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16548.1"
                     /translation="MTLLPAIFLAASLTAAPVPLRTAGGPAARSGEAESAGARFAVVC
                     LDADLLACSPCGASLLEFNRTVPPDILEERVVGVLSFREDAAVPDPRKARLARTRWTG
                     YCRANGIRFRAAVDETHALDPLSERGMTILLFDAGAGILKRWTAPFGPGVLEDIIRSL
                     SVKKQL"
     gene            complement(13062..14012)
                     /locus_tag="PKV64_05395"
     CDS             complement(13062..14012)
                     /locus_tag="PKV64_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16549.1"
                     /translation="MKHRLVFALFVLSAAAVFAAGKDLPAVRVGLEELAAIGGPESDA
                     LFLWTGISVDRDGNVYFSDALDCSLKKFGPDGALLKRAGRKGRGPGEFDKPVGLAVVG
                     ERVYAWDLYDRSVQVFDRDLDYRLTLPMPGSVDALAVLPGGEIAACVRASLVLTKVVV
                     FRPDGTPVREFDLTDPKDEMAPGSISLAVDPSGAAYYGYLFRNLVEKRSLDGERIWSR
                     EILGAGPGVSGNVQGIPLPAETCILSLARDGRGRLFVLGGSAAGRPGRDVFVLGPEGA
                     VLGSFVLPEPSHSVYFDHRDFLYVSADGGVTMKKYRVTFK"
     gene            complement(14112..14321)
                     /locus_tag="PKV64_05400"
     CDS             complement(14112..14321)
                     /locus_tag="PKV64_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16550.1"
                     /translation="MKNLRRLMFRSAAVLLLLVFAAGAAVLEAGVCLDAYTLCMLDPR
                     NSATLVGSLYCANGFVFCVRYVDKK"
     gene            complement(14326..14574)
                     /locus_tag="PKV64_05405"
     CDS             complement(14326..14574)
                     /locus_tag="PKV64_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16551.1"
                     /translation="MIKKKKTVGTRIAAVLLAAVLLGGAAAGPGLAETKNVCQAALER
                     CLADALAGMFGGLVGIIGSLSSCLGGYDFCRKYIISSK"
     gene            complement(14738..16408)
                     /gene="hutU"
                     /locus_tag="PKV64_05410"
     CDS             complement(14738..16408)
                     /gene="hutU"
                     /locus_tag="PKV64_05410"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPN16552.1"
                     /translation="MTPKNTKYRPVRAPRGSRLHTRGWSQEGALRMIMNNLDPQVAEQ
                     PQDLIVYGGTGKAARNWDCYHAIIKNLKSLENDETLIVQSGKPVGVFKSHPEAPRVLI
                     SNAMLVPKWATWDEFRRLEGLGLTMYGQMTAGSWIYIGSQGILQGTYETLAAAAKKHF
                     GGSLKGTITVTAGLGGMGGAQPLAVTMNDGVAIVVEVDRHRIQRRLDTRYVDTMTDRL
                     DEALRLAEDAAKKGVPLSIALLGNAADVLPELVRRGFTPDILTDQTSAHDELNGYVPN
                     GLAYEDALILRTKDPADYIRRSMAAMAAHVRAMLELQKRGARTFDYGNNIRGQARQAG
                     VADAFDIPGFVPEYIRPLFCLGKGPFRWAALSGKPADIYATDQAVLETFPEDEALARW
                     IRKARAQVKFQGLPARICWLGYGERARFGEVINRLVRKGKISAPIVIGRDHLDTGSVA
                     SPNRETEAMRDGSDAVADWPILNALLNAVNGASWVSVHHGGGVGIGFSIHAGMVIVAD
                     GSAAMGRRLERVLTGDPGMGVVRHADAGYPEAVAFARKAGVKIPMARR"
     gene            16537..18531
                     /locus_tag="PKV64_05415"
     CDS             16537..18531
                     /locus_tag="PKV64_05415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPN16553.1"
                     /translation="MGKIFVYSSNPAEREFLFNLAHERGIAYTSPTIEKASSLLAASS
                     FDLAIVELESAGRSPLAESLSSVTCLFLAGDREDLLRDAVRKYPVGRFVDYLLLTGSP
                     SERLRAEHVLNTAQEYARLKKDVESLVKSKASTEEALRNVYAEIKSISSALSQGLVKE
                     LEKRVALEARYIRVQRLNRQFEDILRKLYAATDVNNLLDTIPDIKEIVRAGSISLYLL
                     EDSDTLGTYLKPLVWDQSFLTHPDFTRHIALLPSVDFASSTARTGKEIALAEAGADPR
                     HSPRYRNLSAPLHSLICVPLKSGSEIVGVLEVYNKEAEPSVFSAEDIQILRGLSEHFA
                     LAVTKLNLIQYDALTGLLRPDPFFEKVIQQVEQTTKRRREAGTFAIVMGDVDWFKHYN
                     DRNGQEAGNRLLRELASTMKSAIREEDLLCRYGGEEFLFFLGGVNSIQEATLLTERIR
                     KAVEDRFFEHQEFQPRRNLTMSFGVTLFPPEQIGRAVSKADLKKITGEAELALAEAKG
                     KREAALGMVDEKRVFKNRVCAYVRDKSAVISKTSLLKSPAGTQAVEKRRDPRFFTSTL
                     CLCRENGGHRVFGTVDLSEGGARIASEMRMAKDIVLDMMIILGQKANRFKAQVVHSER
                     ASSQSAFYHTGLRFHDLTEADRKVLRDYFTALEKKGTPLA"
     gene            complement(18538..19875)
                     /locus_tag="PKV64_05420"
     CDS             complement(18538..19875)
                     /locus_tag="PKV64_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16554.1"
                     /translation="MSKKTNVLDESDSLITEIESSLEKNLVKRREEIERELQARIRQE
                     KEESERRLSAVESEIAREKETLQEYRGAISAFEVESANLKSEIKSLLKRSLGEQKDIE
                     QLTTLSQNELRLLSKMTARLAELREQAEMKVSEIRVRLKDKYGLVIEPLKAGEAGDVV
                     VNLQQELSKLKKIQELLETDTPPVETIPPAVSGNTVPETQPEAESFSRLETSFIPPEM
                     SVPESISPEPATDQFRMPAISQFIEEFVRKESEPAADEPLSELPLPKLAAGEPPAPVQ
                     SAEEVNFEAVYEMLEKTRLSEPTDYNGEISFFRNAGTTILDGESVIRAMSHLVDDGRR
                     IYQKLAGMESPKDQFFLKQDLINHQEILRKIILRGVKLCEKEDSRLPRYTDEVVSVRV
                     LKEVLDKLNLDNWSNEEEFSAFESLTLKMKDAFYKRITPPAPYLKSIVKELGL"
     gene            20115..21215
                     /locus_tag="PKV64_05425"
     CDS             20115..21215
                     /locus_tag="PKV64_05425"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPN16555.1"
                     /translation="MTTRKIILFESRAARARSLERVLESLADVVAVPSLSGAARQALD
                     DPEAAILVAVVGPLDEPAARLLSGLKKARPDLSIIAGTRPSDFLTAVEMISAGGLDGA
                     FSPDDVPGAFTAVRSEIQKKDLEERRLSASRTLRRLKVERARSDEQRRELEEIYETTL
                     ENLMTAMDIRDIETFGHSQTVAKYAEALARRLGDRDDGKLENLRKGALLHDIGKIAIP
                     DAILNKPGPLSPEEWDKVRLHPTLGYGLIREIKLVPEIGNIILYHHERYDGTGYPHGL
                     AGGEIPREARIFAVADALDAITAHRPYRKPRDMAAARKEILRNSGTHFDPRAVDAFCS
                     IDLGEWEKIRFETTKQIPFTEDFFRLFRKVKK"
     gene            complement(21225..22070)
                     /gene="prmC"
                     /locus_tag="PKV64_05430"
     CDS             complement(21225..22070)
                     /gene="prmC"
                     /locus_tag="PKV64_05430"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPN16556.1"
                     /translation="MTAAELIGRAVARLRALPGIDPYLEARVLLRRAAGFTDFEIFAF
                     PGRPVAAAAARRFLRAVEDRRGRVPLAYILGEKEFWSIPFTVSPAVLIPRPESELIVE
                     RALELVQAPDPLIIDIGTGSGCLAVALAKEIPGARVVATDISRRALRVAEANAARHGA
                     GNIRFAAGDLFGALRGLGLEGRADLIVSNPPYVTEEEWKRLAPEVRAHEPRRALVPGP
                     TGLETIRRLARGGPRFLKRGGVLLVEFGAGQAGAVRDLFGPPWTRSETARDLSGCPRV
                     AAAVL"
     gene            complement(22067..23158)
                     /gene="prfA"
                     /locus_tag="PKV64_05435"
     CDS             complement(22067..23158)
                     /gene="prfA"
                     /locus_tag="PKV64_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPN16557.1"
                     /translation="MIPELESVEDKYRRLVQALADPSVLSDPKKFRDLSKERADIEPL
                     VKKIEELKRVRADIAGSREILSDPRADPDLKTLAEAELRRLEEADAVLAHDIRILLVP
                     KDPLDERGVVLEIRAGAGGDESSLFAQDLFRMYARYAEKKGWLFEILETSFSPVGGFK
                     EAIAGIRGTGAYSRLKFESGVHRVQRVPKTEAGGRIHTSTVTVAVLPEADEVDIKIDP
                     KDLKIEAFGSSGPGGQNVNRNYTAIRVTHKPSGMVVSCQDEKSQHRNKEKALRVLRSR
                     LLEIARTEQQRKIASDRRSQVGTGERSEKIRTYNFPQSRITDHRLDENFHNPAAVLDG
                     ELEAILEKLFRQDQDRRLEEARAAGGPDR"
     gene            complement(23177..23398)
                     /gene="rpmE"
                     /locus_tag="PKV64_05440"
     CDS             complement(23177..23398)
                     /gene="rpmE"
                     /locus_tag="PKV64_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPN16558.1"
                     /translation="MKQNIHPNYDDCLVICACGNTFQTRSTKKEIRVEICSQCHPFFT
                     GKQKFIDSAGRVEKFRKKFGPGLAAKKKK"
     gene            complement(23467..>24175)
                     /locus_tag="PKV64_05445"
     CDS             complement(23467..>24175)
                     /locus_tag="PKV64_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16559.1"
                     /translation="AKMGFYKEARKRGLWPIVLELDSSAYPPLQRFQIFHRENRPDRL
                     IVDLKIREGVFDPKGKTLAPAPLRPFSTLVFEWLTLQNPEARFSERHGSLPGQLYPGL
                     GMSKRIMDIFIYLGRTAGKEGLQAFPAYYHNAVLFSRYFKFADPAKEAEVQTIRRTFS
                     HVPIRQMAWIVHRNCLKRLDGSPYEWKAEEQIFPLVKDVREHFESRAYRDRVRENMNR
                     TRFVVDWEEFERKSQED"
BASE COUNT         4365 a         7695 c         7561 g         4554 t
ORIGIN      
        1 cccgaatccc tgagctcttt cagacggccc aggacaagcg ccatttctcg agagtcgttt
       61 tcgtccccgt tatgcgcgga acggagggta tcgaatatca gcaaggcgcc cgcgggaagg
      121 cgcttataga gctcgaatcc ggccgagtcg atcttcggcg gccgaggatc gattgccgag
      181 gtccagaata gaacgtccgg tatttcgaga gccttgatcc gttcgaccag aaccgggagg
      241 ggattttcga aatcgacgta aaccacgggc cggggaagcg tcggaaggtt gaaaaaatcc
      301 tggcccctgg aaacggcctt tccgatctcg agggcggccc aagtcttccc gattccgccg
      361 cggccaggga gaagagtaat cgaatcggcc gggacaagac cgcggataag ccattcgatc
      421 cggatatcaa aagcctggag ctcctccccg gtcttcaaga ggcgagccgg atcaccggcg
      481 ccggcgattc ccgataactc ctcgagcagc cggaacgatt ctcgagcccg ggcgaaactt
      541 tccggatcaa cctccccggt tttctcctcc tcatgcagca ccttttggag ctccacaagt
      601 gcccggatcc ggagcgcctt ccggcggagc tcaaagatgc ggccgcggaa tgattcgaaa
      661 tcaagccgga tatttccatc gatcaagccc gcgacaaagg ccgcggctcc gtcccctttg
      721 attttttcgg ataaaagaat cggatcaatc gacttcgggc ggccgtcttc ccaaagctcg
      781 gcgattttag aaaaggtttc ccgaagacgg ccggaaggaa aatcgtttcc ggaaatgtcg
      841 gaaagctcga gtagctcagg tttgagaatg agcgccccga gaatcgatcc cgggagggaa
      901 aggccgcggc tcacgttgtc cgccgactct ttgcctgggg gggctcccct ttggcttgtc
      961 tttcaaggtc cgattcgagg gcggagaaat cgacgcggac cgctcggccg atcttcccgg
     1021 ccggaatttt cccttgggag atcaggcggc gaacgctgac gggatgtaag ccaagggcgg
     1081 cggctgttgc gtttaccgaa taccagcgcc gagcgttcat ggtttccccg ggaataggac
     1141 ttcttcggga acctgcaagg ccttgcaaag cgctcggcgt tctctctcgc ttggggagag
     1201 ataaccgttt tcgattcgag aaattcggga tcgatcgact ccggcccggc ggccgagttc
     1261 atcttgcgaa agccgggcga gatcccggaa gctgcgaagg tgagaaaggt ttttcattcc
     1321 gtgcctccaa ttccatgcta cggaatgaat cccggaccgt caatcacaat ttgtttatta
     1381 taaagaagtt atgcctgtat tagagggagt ctttattaca ggcaaaaaca gcttgggttt
     1441 ataattctct gatcagcaat gttatgtttt ttatcgagct ttcaatcgtt tttcgaggga
     1501 tcttttcttt ccgatggaga aaccaggcca tcgcaatttt cccggcgctc agcttcccga
     1561 aatccttccc cctgtgaagg tcgtaccagc acaaatcccg ttcaaatgtt cgtcgttgtc
     1621 gacccccata aatttccccg aatacttttt tttgagcgta tcggacttca agccaaagat
     1681 aatctatgtc tttttttttc gtccagggat gaatccgaat atagatatag ccgtcttcat
     1741 cttggataga atgatgaagg gcatctccaa tccctcgcca aggtccagcg tccggataga
     1801 agctccatga tttcaattcg acttgcttct ttgatacccg ggccctggga attgagacat
     1861 ggagtgaggg gagggaatat gtacattcaa ccaaaccttc taatcttcca ttccatgctt
     1921 cggatattcg ccatttatca cagaacgcct tccattcatc cgaatggctg gggaatttct
     1981 ccgcagcttt gtgttccttc cgaaaacgga agtttctctt cataagttcc gcccgaatct
     2041 cagcggcctt taaccgccgc ttgctttcct gaatcctttt ttcaatttct tctttcacgt
     2101 caactcccac tgtcaagaat tatgacgccc cgggcgctat catcggctat tttctgcata
     2161 cttggactat atagcaagtc ttcgagcgaa cagagaaaaa cgcctgataa atcagcctat
     2221 cagggggaaa aaaaggaatg acccgtatag gacttgaacc tataacccgc ggattaagag
     2281 tccgctgctc taccaattga gctaacgggc catcgaggga aagtagtgta gccgagaggg
     2341 ctttcgttgt caaacggcct gaattggtca gaatgatcgg cgatccgcgg cgatcttgcc
     2401 ggaaatgtct cggcttccgg ggaccgggat tctcgagcgg aacaatgcgt caggtcccgg
     2461 cggaatttca cgccgcgacg ggcttttccg gccctccggc gatcttctcc aatccggcga
     2521 tcatttcggg cgcggcgatg aaaaccaaca cgtcgccggc ttcgatccgg gcgttttttc
     2581 cgggattgaa gtcgaaatcc ttcccccctc cccttcttcg ggcgacaaac agggccccgg
     2641 tccgggattc gaaatccgcc tcgcccaccg tcttgccggc cagcggcgat ccgggcggca
     2701 cggtcacttc gtcgaaccgc aaaacccggc ccctctcccg gagcatcagg tcgaggaacg
     2761 tggtcgtcgc ggggcggacc atctccgaaa ccatccgcat cccgccgatg aacgccgggg
     2821 agatgacgct gtcggccccg gccttcttca tcttgtcgtg cgaccggaca tcgatcccct
     2881 tggccacgac ccggatcgcg ggattcaagc tcttggccgt cacgacgacg aagaggttgg
     2941 cctcgtcgct gggcaataag cagagaatcc cccgggcccg gccgatgccg gccttccgca
     3001 ggacgtcgtc ctcggacgga tcgcccagga tccacgcgat ttcccccagg gtcttcatct
     3061 tctccatccg ctcggccgac ggatcgacga cgaggaaggg ctttttcgtc tggaggagct
     3121 cctggacgac ggtttgggcc gtctcgtcgt tgccgcaaac gatgtaatgg tttccgagat
     3181 gggcgatggc tttttccatc ttatgtctcc ccagaaggtt ccggagctcg ccctccacga
     3241 cgaacgcggt catggaggaa acggcgaagg cgatcgtccc caggcagagg atgacgtaga
     3301 cgacgccgaa cgcccgggcg gccggatggg cgctcaggtc atggacctcg ccgtaaccga
     3361 ccgtggccac ggtgatgacg gtcatgtaca tggcgtcgag gatcgtccat tcgggcccgc
     3421 cgaggatttt gaagccggcg accccgatga cgaagacgag aaggaagacc gtcagagcgg
     3481 cgacgaggcg ctttctgctg gccatgggcg atgatggcgt gcctgacagg attcgaacct
     3541 gtgacctacg gctccggagg ccgtcgctct atccaactga gctacaggca cgcgggaacg
     3601 gtccgtattg taaccgaggc cgcccccgga ggcaagggcc gccccgcgcc ggctgaaatc
     3661 ccgcccggga gcgagggaca aagccatgcc cgcccgcgga caaggcttta aacggccgga
     3721 gagaatatcg gcgggaaacc ggacgttttt ttcaaacagg ggaaggaaat ggacggccgg
     3781 atcgtctata atgacgagtc cgcatctttc cgaaggagtc cgcgccatga agagacatct
     3841 cgtcggggcg gtcgccgtga tcgccgcttt tctcaccctc caggcccaaa tgccgcccca
     3901 gcctccgttg aagctcgagg acgtccagaa agccctttgc ctcgtggacc ggcccgctcc
     3961 ggtccccgag aaatcccggg ccggcttcga gtccatcact ccccgggaga cgacggccat
     4021 gctcgcctat ctctcctccg attggatgga aggccgcgag accggcacct ccggctacgc
     4081 ccaggcggcc gattacgccg cctcgctttt taaaatgtgg ggcctcaagc cggcaggcga
     4141 tttcccgccc gcgccccgcg gcccgcggcg cttcgggtcg accccgccgc ccccggccgc
     4201 cgagaggagc tatttccaga acatcgcctt caaggagatc tccgacgtcc ggagcactct
     4261 caccgtcgaa gtcgccaagg gccccggcgt taaaacgcgc gcctatgcgg gcggcgtcga
     4321 tttcgcgatc gttccgggac cggccgtgac gatttccgcc ccggtcgtgt tcgccggcta
     4381 cggcatcagc gagccgacgg ttgggttcga cgagctcaag ggcctcgatc ttaagggaaa
     4441 gatcgtcctg atcctgacgg aggccccggg caaggacaat ccccagtccc tcttccaagc
     4501 cgacaaggcg cttaaggaaa aatacttccc gacattcgcc ggcttcgaaa tgatgatgcg
     4561 ccgcggcggt cctccccgct tctccaaggt cgaggcgatc ctcgcccggg gcgcggcggc
     4621 cgtcgtccag gtcgacaaca cctcccggga tcgggagatc ttccgcgacc tggccacgcc
     4681 ggagaagccg tccgacgacc gtccgatcat ccgcaagccc cggcttcgcc tccaggtcgc
     4741 cggagacgcc tccgcgccca tgggcgggag ggcggcgctg accgtcaccc gggagatcgc
     4801 cgacgcgatt ctcgagggga cgggccaaac ggtcgacgaa ttcaagaaga agatcgaaac
     4861 ggccttcaag ccggcctcct gcctcgtgcc gggaacgaag ctgaccatcg cgaccacggc
     4921 ccggacatcg ctcgtccaga gccccaatgt tctcgccgtg atcgaagggt ccgatcccga
     4981 gctcaagaag gaatgcttcg tcatcggggc ccatttcgac cacctcgggg cctgggaaga
     5041 ctacgtgttc aacggagccg acgacaacgg ctcgggctcg gccggcgtcc tggccatcgc
     5101 ccgggcgatg gccgtcaatc cggtcaagcc gaaacggtcg gtcgttttcg ccctctggac
     5161 cggcgaggaa aagggcctgt acggttcccg ctattacgtc gcccatccct tctttcccct
     5221 cgagaaaacc gtcggctacc tgaattacga catgatcagc cgctcttacg aggcggccgc
     5281 cctggccctg gccgtccggc gctacagcgt cccgggagcc gaggaaatgg tcaagaagat
     5341 ccggcctgac cggttcgcca gcgtgttcgt gacggaaggg acggatctcg ttcgcttggc
     5401 caaggaaatg aacgcgttcg tcggcctgga tttggccttc cgggaaagcc ccctcggctc
     5461 ggcgggcggc gggtccgatc acgccccgtt ctcccaagcc aagatcccct acgtctattt
     5521 catgggagcc atgacggagg attatcacca gacgagcgac agcatcgaga agatcaatcc
     5581 cgagctgttc acgaaaatct gccggatcgg ctacctgacc gcgttcgccc gggctgatca
     5641 ataatccggt ccggcggccg cgctgttttt cgatccgtct cccgcgcccc gctccccttc
     5701 caaggggggc ggggcgtttt tttccgtctt cggccggggc gcatcgactc acataacgtc
     5761 tgaccgaacg ccgggctgtt gaatcctaga aaaacgccgc cgaagaagga tcgggatttc
     5821 ggttacatat tagttgattc aatgtgcagg gttgcggacg ggcccggctt cgcatatcat
     5881 ccgtttttga acgccatgcc cgaacacccg acggccccga agcgtccccg catctgggag
     5941 atcgatttcc tccgcggcct gtccatcctc ctgatggtcg gctatcatct cctttacgac
     6001 ctgggagcct tcgtcggcct gaagaggttc ctcggctttt caaccgacct cttttccccg
     6061 gcctggatga tcgcccagta tttcttcgcc gggcttttcg tcgttctctc ggggatcagc
     6121 tccaccctga cccggagcaa catccgccgg gggctgcgtc tcctggtcgt ctccttggcc
     6181 gtcacggccg cgacttatgt cttcgatccg ccctcgacga tctggttcgg aatcctccag
     6241 tgcctggccg tctcgatgct cctctacggg gcggccttcg agaaagcccg tccggcggtc
     6301 tcggccgcgg ccggggcggc cgtcttcggg ctcggcttcg cccttccggc cgtccggcgg
     6361 gccctcgtcc ttcgctcgga ttggctgctt cccctcgggc tccacagccc cttgttcatg
     6421 tcgtacgatt atttcccgct gattccgtgg ttcggcgtct tcctgatcgg agcggccttg
     6481 ggaaagaccg tgtacaaatc ccgccggagc ctgctgcccc ggcctccgcg gccgatcttc
     6541 atcaacgccg ccggccgtta ttccctctgg atctacctcg tccatcagcc cgtcatcatg
     6601 ggcgttcttt atcttctcgg gctggttcga tagggacggt cctccaaaag ccgacaagcg
     6661 cggacgacgg ccgtcccggc cgccggatcg atgatgatga atccgcgccg cccggggaaa
     6721 aaagctttcc ttcgtttggg cggaatccgc tcagccgtcg gcccgcccgg ttttctggag
     6781 cttggtccag acgtcgcgca gggtgaccgt ccggttgaag acgggagcgc cgggccgcga
     6841 atgcttcgtg tcggcgacga aataacccat ccgctcgaac tgccagcgcg agcccggggc
     6901 ggcctcggcc agcatcggct cgagccgggc ctcggagatc gcctcgagcg acttggggtt
     6961 gaggttcgcc agccagtcct ccccttcctc cagctcgttc ggatcgcgct tggtgaagag
     7021 ggtgtcgtag agccggactt cggccgggac ggcgtgggcg gccgagaccc agtggagggt
     7081 ggccttggga ctccggccgt cgggggaatc gccgccgcgg gtggccggat cgtatgtgca
     7141 gcggacctcg acgacgccgc ccgtggccgg gtccttgacg acctcccggc aggtgatgaa
     7201 gtaggcgtag cgaaggcgga cttcgcggcc gggcgacagg cggaagaatt ttttcggcgg
     7261 gtcttcgagg aaatcctcgc gctcgatgta aagcgtccgg gaaaacggga cggcccgcga
     7321 cccggccgag ggatcctcgg gattgttgac gcactcgagc atctcgacct tgtcctcggg
     7381 atagttggtc aggacgattt tcaggggccg gaggacggcc atcgcccggg gcgacgtttt
     7441 gttcaggtcc tcgcggacac agctctcgag gaggggcata tcgacgatgc tcggcacctt
     7501 ggccaccccg atcaggtcgg cgaaccgccg gatggcggcc ggcgtgtagc cgcggcgccg
     7561 caggcccgag atcgtcggca tgcggggatc gtcccagccg tcgacccggt tctccttgac
     7621 gagcaggagg agcttccgct tgctgaggac ggtgtggctg aggttgagcc gggcgaattc
     7681 gatttggcgg ggcttgaaga cgccgagctc cttcagaaac cagtcgtaca gcgggcggtg
     7741 gacctcgaat tcgagcgtgc agatcgaatg ggtgatcccc tcgatcgaat cggactggcc
     7801 gtgggcccag tcgtaagtgg gatagaggca ccaggcgtcg ccccggcggt agtgatgggc
     7861 ccgccggatg cggtacatga ccgggtcgcg gagaagcagg ttgggatggg ccatgtcgat
     7921 cttggcccgg aggctcttgg ctccgtcggg gaattcgccg gcccgcatcc gccggaagag
     7981 atcgaggttt tcctcgaccg tccggttccg gaaggggctg tccgtccccg gccgggtcag
     8041 ggtgccgcgg gtcgcggtga cgtcctcggc gcccaggtcg cagacgtagg ccttgccctt
     8101 ccggatgagg atctcggcga attcgtagag cttctcataa taatcggacg cgtagtactc
     8161 gcggtcgtcc cagtcgaacc ccagccagcg gacgtcctcc ttgatcgagg cgacgtactc
     8221 gaagctttcg gtctcgggat tggtgtcgtc gaaccggagg ttgcagaggc cgtcgaaatc
     8281 ggcggccaag ccgaaattca ggcagatcga cttggcgtgg ccgatgtgga gatagccgtt
     8341 cggctcgggc ggaaaccggg tgtggacgcg gccctcgatc cggccggcgg cccggtcttc
     8401 ccggacgatg tcccggatga aattttcccg cggtttcttg tcgtccgttt cggtcatagg
     8461 gaattccttc gcaggcggtc gacgaccgtc ccccgcccga gaacggccat gatctcgaac
     8521 aggccggccc ccttggtcag gcccgagacg gccatgcggg acggatggat gagctcggcc
     8581 accttgatgt tccgctcggc ggccaggccc cggtagagct cctccaggcc ggccgcgctg
     8641 aattcgggca gagcttcgag ccgggacgcc agatcggcca gcagggcttt cttttcaggg
     8701 gcgccgaggt gttttttccg gccttcctcg tcgaccggaa agtcctcccg gaaaaagaat
     8761 tcgatcatcg gccccagctc gcgcagcgtc ttgatccgga tcttgtacag gtcgacgacg
     8821 gccgcccagc gggcgccgtc ggaaacgtcg aaatcatatc cgtcgcggcg gagctggtcc
     8881 tggatcaggg ggacgagagc cgccgtctcc ttggccatga tgtattcgcc gttcatccat
     8941 ttcagcttca caaggtcgaa cttggcctgg acgtcgttca tccggtcgat ggagaacaga
     9001 cgggcggctt cggccaggcc gaggatctcg cggccgtcgg agggcgtcca gccgaggaag
     9061 atcaggtaat tggccagggc ttcgggcagg aagccctcgg ccttgtattc ggccaccgag
     9121 acgccgccgt gccgcttgct gagcttggag ccgtcgggcc cgaggatgag gggcatgtgt
     9181 ccgaaccgcg gcgggacgag gccgaacgcc tcgtagaaga ggatctgctt gggcgtgttg
     9241 gagatgtggt cgtcgccccg gaggatgtgg gtgatcccca gctcggagtc gtcgacgacg
     9301 cagcagaagt tgtaggccgg cgagccgtcg gacttgatca ggacttggtc cttgatgttt
     9361 tccgtctcga cctcgatccg gccgtggatc aggtcgtcgt aagcgatttt ccggccgggg
     9421 tcgacccggt agaggatcgc ctccccttcc cggtaggccc ggccggaggc caccagggat
     9481 tcggctttct cccgatagag atcgaaccgg cggctttggt acatcggctc gccgtcccag
     9541 tccaggccga gccaggccag gtcctccagg atctcgtcca ggaaccgctg ttcggagcgg
     9601 accttgtcgg tgtcttcgac gcggagaagg aagcggcctc cggagctgcg ggccgccagc
     9661 cagttgaaca gggcggtccg ggcgcttccg agatgcagaa agccggtggg gctcggggcg
     9721 aaacgaacgg ttaagggcgt cttcacggag aaaaaacctc tgcgggattg tatagcacct
     9781 ccctccatcg gtcaaacttc ggcaaggttt ttaagatgat ttccccgaag cccgggccga
     9841 gacatccgcg gccggaaagc ttttttgcta taatatcggg ctatgttgat acgaaaagcc
     9901 cgcccggagg acttcccggc gatcaggaag ctggccgagg cgctcggcct cgattacccc
     9961 ggcatggaaa aagccctgtt ctggctggcc gagaaagacg ggcgcgtcct cgggatcgtc
    10021 tcccttctcg ttcacgccga cagccgggag ctcgtctccc tcggcgtcga tcccgcggcc
    10081 cgggaaaagg gcttgggagg ccggctgatc gagacggcgg tggcagccgc cggggcggac
    10141 gtttacctgg ccaccgtcat tcccggctat ttcgagcggc acggattcgc ccggacgctc
    10201 cgcgtcccgc gggggatggc caaggatccg gcctggtgcg cgggctgcga caagagccgc
    10261 tgcgccgtca tggtccggga gaaatcatga gccttccggc ctttcccgaa ttcaagcccc
    10321 tggcgctcga ggaccgttcc gtcgtccagg gatttttcga ccgggcggcc gtggaagcct
    10381 gcgagtattc gttcgggaac aatttcatct ggcggcattt cgaaaagacc cgctggacga
    10441 tcctcgacgg gaatctctgc ctcgtgttcg actgtcccgg gcggccgccc tacgccctcg
    10501 cccccgtcgg cgggacggct gcggccggcg ccgtccgggc ccttctcgat gtcgtgcccc
    10561 ggctggcccg ggttccggcc gcgttcgtcc aagagcatgg ggcggacttc ctttgcgagc
    10621 ccgagcgcga cgagttcgat tacgtctacg cggccgcgga tctggcggac ctggcgggca
    10681 agaaatacga cggcaagagg aaccggatcc ggaaattcga gcgcgagcac caatggcgtt
    10741 acgtccccct ggacaacggt cacctggacg ccgcccggcg gctgttcgag gagtggttcg
    10801 ccgagcggga cgatcactcg gacatggccc aggggcagaa gacggccatc tcggaggcct
    10861 tggcccattt ccgcgagctc ggcctcctgg gaggggcgat cgaggtcgac gggaggttcg
    10921 ccgccctttc gatcggcgag cccctcaccc gggacacggc cgtcatccac atcgagatcg
    10981 tcagcccctg ctgcgagggc ctggcccaga tcatgaaccg ggaattcgtc cgccacgcct
    11041 tgaaggattt cgccttcatc aaccgcgaac aagacctcgg cctgcccggc ctgcgccggg
    11101 ccaagctctc ctaccacccc catcatctcg tcgagaaatt ccgcctcgag cgccggccct
    11161 gagaggctca atccttccgg gcggcgagct cggcaacgtt gcggatgtac cggtcggcgt
    11221 cgaatttcct ccgcaggccc gcctcgttca tgtagcggac caggccgaag atcggacgct
    11281 cgccccaagg acggtcgtgc ttgccgaagc accaggccac cccggcgaag ccgctgggat
    11341 cccggccgtc gatctcgtac ttgttgttga gatagagggc ggtcgcgtag gcttcttccg
    11401 gcgaggcgct ccactccagg attttcttcc cccaatacat ccgcatgtag ccgtgcatct
    11461 tgccggtcac ggccatttcc cgctgggccg cgttccagca aggatcatgc gttttcccgg
    11521 cctccagctc ggcccgcgtg tagaggacgg gccggggatc ggccgcgtgc ctggccaggg
    11581 tcgcccgggc ccaggccggc agcgattcaa acgtgtcgta gcggtcgttg taatgaacga
    11641 aattcatggc cagctcccgc cggacgacca gctcctcgag gaaggcctcc cggccgcggc
    11701 tgttcgacgc ggccgccttc gccgccgccc agaccggcga gatctggccg aaatggagat
    11761 aagggctgat gtgggagagg aaatcgacgg tcggattgtt gcggagatcg gcgaaatgat
    11821 cgagccggtc ccggaggaaa aggcggaagc gcctcttggc ctcctccgtc ccgccccggt
    11881 agaagggcga cgggccgacg ctccggtcga tcttcaagcc ggccaggacg tcctcgatgt
    11941 tctcgaggtc gatcgagtcc cccttccatc ccagggaatc ccgaagcggc ttccggtcgg
    12001 gggaaacggc aagagaggaa tccaggatct tccggatctt cggacggaac gtggccgccg
    12061 agtattcttc cttgggcgaa gccttctcca cgggaaccac ggtctcggtt tccacttgga
    12121 tcaagggaca agccaaggcg gcggccgccc gctcccgcca ggatttctga atccggaggt
    12181 atccgcggtc ggtcacgacg agcgaagccg cccgccccag ctcgacggcg ccccggtccg
    12241 gttgaatccg ccggacgacg agacggacgc cgcggtcggc cagggccagc tgcgtttccc
    12301 gaaggccctc gagcatgaag gccaggtggc gggcgttggc ttcgggaaag cgctcggtca
    12361 agccgaaaaa ggccaggcag gggcggcgct gctcgttggc cagccggacg gcgtgctgca
    12421 gggccgggtt ggcctcggcc cgctgggccg cctgcatcca gtagagcacg taatcgccgc
    12481 tcttcgtttc ccgggaattg aggacgcgca gacgttccgt tttgatcatg ggcgggtcta
    12541 ttatagctgt tttttgacgg agagagagcg gatgatgtcc tccaggacgc cgggtccgaa
    12601 cggcgcggtc catcgcttca ggatccccgc cccggcgtcg aaaagcagga tcgtcattcc
    12661 ccgctcgctc aaggggtcga gggcgtgggt ctcgtcgacc gcggcccgaa agcggatgcc
    12721 gttggcccgg caatacccgg tccaccgcgt ccgggcgaga cgggccttcc gcggatcggg
    12781 gacggccgcg tcctctcgaa aggaaagaac gccgacgacg cgctcttcca ggatgtccgg
    12841 aggaaccgtc cgattgaact ccaagagaga ggctccgcag ggggaacagg ccagcaggtc
    12901 ggcgtcgagg cagacaaccg cgaagcgggc gccggcggat tccgcttccc cgctccgggc
    12961 ggccggcccg ccggccgtcc gcaagggaac gggcgcggcg gtcaatgacg cggccaaaaa
    13021 gatcgccggt aaaagggtca tgatccggat ctccccggcc ttcacttgaa ggtcacccgg
    13081 tatttcttca tcgtcacgcc gccgtcggcc gagacgtaga ggaaatcccg gtggtcgaaa
    13141 taaacgctat ggctcggctc gggcagcacg aaggaaccca agacggctcc ctccgggccg
    13201 aggacgaaaa cgtcccggcc cggacggccg gccgccgaac cgccgagaac aaagaggcgg
    13261 ccccgcccgt cccgggccaa gctcaaaatg catgtttccg cgggaagggg aattccctgg
    13321 acgttgcctg agacgcccgg gcccgctccc aggatctccc ggctccagat ccgctctcca
    13381 tcgaggctcc gcttttccac gaggtttcgg aagaggtatc cataataggc cgcgcccgag
    13441 ggatcgacgg ccaggctgat cgagccgggg gccatctcgt ccttcggatc cgtgagatcg
    13501 aactcccgga cgggcgtccc gtcgggacgg aaaacgacga ctttcgtcaa aacgagggat
    13561 gcgcggacgc aggcggcgat ctctcctccg ggaaggacgg ccaaggcgtc gaccgatccg
    13621 ggcatcggga gcgtcaggcg gtagtccaaa tcgcggtcga agacttgaac ggaccggtcg
    13681 tacaaatccc aagcgtacac ccgctcgccg acgacggcga ggccgacggg cttatcgaat
    13741 tccccgggtc cccggccctt gcgccctgcc cgtttcaaga gggcgccgtc agggccgaac
    13801 ttcttgaggg aacaatccag ggcgtcggag aaatagacat tcccgtcccg gtcgacggaa
    13861 atcccggtcc agagaaacaa ggcgtcgctc tccgggccgc cgatcgcggc cagctcctcg
    13921 agcccgaccc ggacggccgg aagatccttc ccggcggcga aaaccgccgc ggccgaaaga
    13981 acgaacaacg cgaaaacaag ccggtgtttc atgatcgcct cctgctggga aggacgctcg
    14041 gccgccgcgt tattttcatt tcccaaaaaa agccggggga ggatgcctgg gatcctcccc
    14101 cggccggccg actacttttt atcgacgtac ctgacgcaaa aaacgaagcc gttcgcgcag
    14161 tacaggcttc cgaccagggt cgcgctgttg cggggatcga gcatgcagag ggtgtaggcg
    14221 tccaggcata cgcccgcttc caggacggcc gctcccgcgg cgaaaaccag caggaggagg
    14281 acggccgcgc ttcggaacat gaggcgccgg aggtttttca tgggatcact tcgaggaaat
    14341 gatgtacttc cggcagaagt cgtacccgcc caggcacgag gacaaggagc cgatgattcc
    14401 gacgaggccg ccgaacattc cggccagggc gtcggccagg catctttcca aggccgcctg
    14461 gcagacgttc ttcgtctcgg ccaggccggg cccggccgct gctccgccaa ggagcaccgc
    14521 ggccagaagg accgcggcga tcctcgtccc aaccgtcttt ttctttttga tcatgggtga
    14581 tctcctttcg gccggatcca atgccggatt catgccaaaa gaaagccggg gaagcacggc
    14641 ggattcgcgg ggaaaacgaa aaagaaagga gacggaaagc gggacggccg tcgccgaacg
    14701 ttgacaagcg ccggcgaagg gaaatccgcc ggccgcctca gcgccgggcc atcgggattt
    14761 tcacgccggc cttacgggcg aaggcgaccg cctcgggata gccggcgtcg gcatgacgga
    14821 cgacgcccat gccggggtcg ccggtcagga cgcgctcgag gcggcggccc atcgcggccg
    14881 agccgtcggc cacgatcacc atcccggcgt ggatcgaaaa gccgatgccc actccgccgc
    14941 cgtggtggac ggaaacccag ctggccccgt tgacggcgtt gaggagagcg ttgaggatcg
    15001 gccagtcggc gacggcgtcc gagccgtccc gcatggcctc ggtctcccgg ttgggcgaag
    15061 cgaccgaccc ggtgtcgagg tggtcgcggc cgatgacgat cggggcgctg atcttgccct
    15121 tgcggaccag ccggttgatg acctcgccga aacgggcccg ctcgccgtag ccgagccagc
    15181 agatccgggc cggcaggcct tggaacttga cttgggcgcg ggccttgcgg atccagcggg
    15241 ccagggcctc gtcctcggga aacgtctcca ggacggcctg gtcggtggca taaatgtcgg
    15301 ccggcttgcc ggatagcgcg gcccagcgga aggggccctt gcccaggcaa aacagcggcc
    15361 ggatatattc gggaacaaat cccggaatat cgaacgcgtc ggccacgccc gcctgccggg
    15421 cctggccgcg gatgttgttg ccgtaatcga aggtccgggc gccgcgcttc tggagctcga
    15481 gcatcgccct cacatgggcg gccatggccg ccatgctccg ccggatataa tcggccgggt
    15541 ccttcgtccg caggattagg gcgtcctcat aggccaggcc gttggggaca tagccgttga
    15601 gctcgtcatg ggccgacgtt tgatcggtga ggatgtcggg ggtgaagccg cggcggacaa
    15661 gctccggcag gacgtcggcc gcgttgccga gcagggcgat cgagagcgga acgcccttct
    15721 tggccgcgtc ttcggccagc cggagggcct catccaagcg gtcggtcatc gtatccacgt
    15781 agcgggtatc gagacgcctc tggatccggt gccggtcgac ttcaacgacg atggccacgc
    15841 cgtcgttcat ggtgaccgcc aggggctggg cgccgcccat tcccccaagg ccggccgtga
    15901 cggtgatcgt ccccttcagg gacccgccga agtgtttttt cgcggcggcg gccagggttt
    15961 catatgtccc ttggaggatg ccctgggagc cgatataaat ccagcttcca gccgtcatct
    16021 gcccgtacat ggtcaggccg agcccttcca agcggcggaa ctcgtcccaa gtcgcccact
    16081 tggggaccag catcgcgttg gaaatcagga cgcgcggcgc ctcggggtga gatttgaaga
    16141 ctccgaccgg cttgcccgac tggacgatca gggtttcgtc gttttccaag gatttcaggt
    16201 tcttgatgat cgcatggtag caatcccagt tccgggccgc ttttcccgtg cctccgtaga
    16261 cgatcaggtc ctggggctgt tcggccacct ggggatcgag gttgttcatg atcatccgga
    16321 gagcgccttc ctggctccag ccgcgcgtgt gcaggcggga gccgcgggga gcccggaccg
    16381 gacggtattt cgtgttttta ggggtcatct catcctcctg gactggaaag aaataatata
    16441 acacaccgcc caaaaaatat cttgacaggg cccaacaatg ggattataaa cgaattggaa
    16501 gccctggccg aggtttgaag cgaaggatag gcgagcgtgg gcaaaatttt cgtgtacagt
    16561 tctaacccgg cggagcggga atttctattc aatctcgctc acgaacgggg aatcgcctat
    16621 acctccccca ccattgaaaa agcctcttcc cttctggccg cctcgtcctt tgacctggcc
    16681 atcgtcgagc tcgagtccgc cggccgctcc cccctggccg aaagcctgtc ctcggtcact
    16741 tgtctttttc tagccggaga ccgggaagat ctgctccggg acgccgtccg gaaatacccc
    16801 gtcggccggt tcgtcgatta tctcctgttg accggatctc cttccgagag gctccgggcc
    16861 gagcatgttc tgaacaccgc ccaggaatac gcccgcctga aaaaagacgt ggagtccctg
    16921 gtcaaatcca aggcatccac cgaggaggcg ctccgcaacg tctacgccga gatcaagagc
    16981 atcagcagcg ccctgtccca gggactcgtc aaggagctcg agaagcgggt cgccctcgag
    17041 gcccgttaca tccgcgtcca gcggctcaac cgccagttcg aggacatcct ccgcaagctg
    17101 tacgcggcca cggacgtaaa caacctcctc gacacgattc ccgacatcaa ggagatcgtc
    17161 cgggccggaa gcatctccct ctaccttctc gaggacagcg acacgctcgg gacctatctc
    17221 aagccgctgg tttgggatca gtccttcctc acccatcccg atttcacccg gcacatcgcc
    17281 cttcttccct ccgtcgattt cgcctcgagc acggcccgga ccgggaagga gatcgccctg
    17341 gccgaggcgg gcgccgatcc gcggcattcc ccccgctacc ggaacttgag cgcccccctc
    17401 cacagcctga tctgcgtccc cctgaaaagc ggctccgaga tcgtcggcgt tctcgaagtc
    17461 tacaacaagg aggccgagcc gtcggtcttc tcggccgagg acatccagat cctccgcggc
    17521 ctgtccgagc atttcgccct ggccgtgacg aagctgaacc tcatccagta cgacgccctc
    17581 accggtctgc tccggcccga tcccttcttc gaaaaggtca ttcaacaggt cgagcagacg
    17641 accaaacggc ggagggaggc ggggaccttc gccatcgtca tgggcgacgt cgactggttc
    17701 aagcactaca atgaccgcaa cgggcaggaa gccggcaacc ggctgctgcg cgagctggcg
    17761 tcgacgatga aatccgccat ccgcgaggaa gacctcctct gccgctacgg aggcgaggag
    17821 ttcctctttt ttctcggcgg cgtcaattcc atccaggaag cgaccctgtt gaccgagcgg
    17881 atccggaaag cggtcgagga ccggtttttc gagcaccagg agttccaacc ccgccgcaac
    17941 ttgacgatga gcttcggagt gaccctgttc ccgccggagc agatcggccg ggccgtgtcc
    18001 aaggcggacc tcaagaagat caccggcgag gccgagctgg ccttggccga ggccaaggga
    18061 aaacgggagg ccgctctggg aatggtcgac gaaaagcggg ttttcaaaaa ccgcgtctgc
    18121 gcttatgtcc gcgacaaatc ggcggtcatc agcaaaacct cgctcttgaa aagcccggcc
    18181 gggactcaag ccgtcgagaa acgccgcgat ccgcgctttt tcacctcgac gctctgcctc
    18241 tgccgggaaa acggcggcca ccgcgtcttc ggcaccgtgg acctgagcga aggaggggcg
    18301 aggatcgcct cggaaatgcg gatggccaag gacatcgtcc tcgacatgat gatcatcctc
    18361 ggccaaaaag ccaatcgctt caaggcccag gtcgtgcact cggaacgggc ctcatcccaa
    18421 tcggcctttt atcacaccgg cttgagattt cacgacctca ccgaggccga tcggaaagtc
    18481 ctgcgggatt atttcaccgc cctcgaaaag aagggcactc ccctggcctg aactccgtta
    18541 gaggccgagc tccttgacga tggatttcaa gtaaggcgcc ggcggggtga tccgcttgta
    18601 gaaagcgtcc ttcatcttca gggtcaggct ttcgaacgcg ctgaattcct cttcgttgct
    18661 ccagttgtcg aggttgagct tgtccaggac ttccttcagg acgcggacgc ttaccacttc
    18721 gtccgtgtag cgcggcagcc ggctgtcttc cttctcgcac agcttgacgc cgcggagaat
    18781 gatcttccgc aggatttcct ggtgattgat caggtcctgc ttcaggaaaa actggtcttt
    18841 cggcgattcc atgccggcga gcttctgata gatcctccgg ccgtcgtcga cgaggtggct
    18901 catcgcccgg atgaccgatt cgccgtcaag aatcgtcgtc ccggcgtttc ggaaaaagct
    18961 gatctcgccg ttgtaatccg tcggctcgga caagcgggtt ttttccaaca tctcgtaaac
    19021 ggcctcgaag ttgacttcct ccgcggattg gaccggcgcc ggcggctccc ccgccgccaa
    19081 tttcggaagg ggcagttcgg agagaggctc atcggccgcg ggctcgcttt ccttgcggac
    19141 gaattcctcg atgaactggc tgatggccgg catccggaat tggtccgtgg cgggctccgg
    19201 ggaaatggat tcgggcacgg acatttccgg cggaatgaac gacgtctcca aacgggagaa
    19261 cgattcggcc tcgggctggg tttcgggaac cgtgttccct gaaacggccg gcgggatcgt
    19321 ttcgacgggc ggcgtatccg tctcgagaag ctcttggatc ttcttgagct tggacagctc
    19381 ctgctggaga ttcacgacga cgtcgccggc ttcccccgcc ttgaggggtt cgataacgag
    19441 gccgtatttg tccttcagac gaacccggat ttcagagact ttcatctccg cctgctcccg
    19501 gagctcggcc aagcgggcgg tcatcttaga aaggaggcga agctcgttct gggacagggt
    19561 cgtcagctgc tcgatgtcct tctgctctcc gaggcttctt ttcagaagac ttttgatttc
    19621 ggacttgagg ttggcgctct ccacttcaaa ggcgctgatc gcccctcggt attcctggag
    19681 cgtttccttc tcgcgggcga tttcgctctc gacggcgctc aacctccgct ccgattcctc
    19741 cttctcctgc cggatgcggg cctggagctc gcgctcgatc tcctcccggc gcttgaccag
    19801 gttcttttca aggctggatt cgatttccgt aatcagcgag tccgattcgt ctaatacatt
    19861 tgttttcttg ctcatggccg atctccagag gccggggaga ggatcttccc tgaagctttc
    19921 ttcaaggaac gctcctcgcg tccttttcgc cctgagtgta cccgaagcga cccgcaagcc
    19981 gcaatccccc tcctcggcga tcacccgggg gatattcttc tcccgccgcc tcacccctag
    20041 gggtgaggcc gggccggggc ggcttttttc ttgatggcgc cccgggaata ttgtataaag
    20101 agcggcggct atccatgacc acgcgcaaaa tcatcctttt tgaaagccgt gccgcccggg
    20161 cccgaagcct ggagcgcgtc ctggaatccc tcgccgacgt cgtcgccgtt ccgtccctgt
    20221 ccggagcggc ccggcaagcc ctggacgacc ccgaggccgc catcctggtc gccgtcgtcg
    20281 gcccgttgga cgaaccggcc gcccggctcc tgtccggttt gaaaaaagcc cggccggatc
    20341 tctcgatcat cgccggaacg cggccgtccg attttctgac cgcggtcgag atgatctccg
    20401 ccggcggcct ggacggagcg ttctcgcccg acgatgtccc cggcgccttc accgccgtcc
    20461 ggagcgagat tcagaaaaaa gacctcgagg agcgccgtct ttcggcttcg cggactttgc
    20521 gtcgtctgaa ggtcgagcgg gcccggagcg atgagcagcg ccgcgaactg gaggagattt
    20581 acgaaacgac cctcgaaaac ctgatgacgg ccatggacat ccgggacatc gagacgttcg
    20641 gccattccca gaccgtggcc aagtacgccg aggcgctggc ccgccggctc ggcgaccggg
    20701 acgacggaaa gcttgaaaat ctccggaaag gcgcccttct gcacgacatc ggaaaaatcg
    20761 ccatccccga cgcgattctc aacaagcccg gccccttgtc gccggaggaa tgggataaag
    20821 tccgcctcca ccccactctg ggctacggcc tgatccggga aatcaagctc gttcccgaaa
    20881 tcgggaacat cattctctac caccacgagc ggtacgacgg gacaggctac ccgcacggcc
    20941 tggccggcgg ggagatcccc cgggaagccc ggatcttcgc cgtggccgac gccctggacg
    21001 cgatcaccgc ccaccgccct taccgcaaac cccgtgacat ggccgccgcc cggaaggaaa
    21061 tcctccgaaa ctccgggact catttcgacc cgcgggcggt ggacgcgttc tgttccatcg
    21121 atctcggcga atgggagaaa atccgcttcg agacgacgaa gcagattcct ttcaccgagg
    21181 attttttccg gctcttccgg aaagtcaaga aataagcgcc gtctttagag aacggcggcc
    21241 gcgacgcgcg gacatccgga caagtcccgg gctgtttcgc tccgcgtcca cggagggccg
    21301 aacaaatccc ggacagcccc ggcctgcccc gccccgaact ccacgagaag gacgcctccc
    21361 ctcttgagaa agcgcggacc tccccgggcc aggcggcgga tcgtttccag cccggtcggc
    21421 cccgggacga gggcccggcg cggctcatgg gcccggactt cgggggcgag cctcttccat
    21481 tcctcctccg tcacatacgg gggattactg acgatgagat cagcccggcc ttcgagcccc
    21541 aggccgcgga gggcgccgaa gagatctccg gcggcgaacc ggatgttgcc ggccccgtgg
    21601 cgggcggcgt tagcctcggc cacgcggaga gctctccggc tgatgtcggt cgcgacgacg
    21661 cgggctccgg ggatttcctt ggccaaggcc acggcgaggc acccgcttcc cgtcccgata
    21721 tcgatgatca gcggatcggg cgcctggacg agctccaggg cccgctctac gatcagctcg
    21781 gactccgggc gggggatcaa cacggccggg gagacggtga agggaatgga ccagaattcc
    21841 ttttccccca ggatgtaggc cagggggacg cggccgcggc ggtcctcgac cgcccgcaaa
    21901 aaacgccggg cggccgcggc cgccaccggc cggccgggaa aagcgaagat ctcgaagtcc
    21961 gtgaacccgg ccgcccgccg caggagcacc cgggcttcca gatagggatc gattcccggg
    22021 agggcgcgca ggcgggcgac cgcccggccg atcagctcgg cggcggtcat cggtccggcc
    22081 cgccggccgc ccgggcctcc tcgagccggc ggtcctgatc ctgccggaac agcttttcga
    22141 gaatggcctc gagctccccg tccaggacgg ccgccggatt atggaaattc tcgtctaagc
    22201 ggtggtcggt gatccgggat tggggaaaat tgtacgtccg gatcttttcg ctccgctcgc
    22261 ccgtgcccac ttggctgcgg cggtcgctgg cgattttccg ctgctgctcc gtccgggcga
    22321 tctccaggag ccgcgaccgg agaacccgga gggctttctc cttgttccgg tgctgggatt
    22381 tctcgtcctg gcaggagacg accatccccg agggcttgtg ggtgacccgg atggccgtgt
    22441 aattgcggtt gacgttctgg ccgccgggtc cggaggagcc gaacgcctcg atcttaagat
    22501 ccttcggatc gatcttgatg tcgacctcgt cggcttcggg aagcacggcg accgtcacgg
    22561 tggacgtatg gatccgtccg ccggcctcag tcttgggaac gcgctgaacc cggtgcacgc
    22621 cgctttcgaa cttgaggcgc gaataggctc ccgtcccgcg gatgccggcg atcgcctcct
    22681 tgaatccgcc gacgggcgaa aaactcgtct ccaagatctc gaaaagccag ccttttttct
    22741 cggcgtaccg ggcatacatc cggaacaagt cttgggcgaa cagggaggat tcgtcccccc
    22801 ccgccccggc ccggatttcg agcaccacgc cgcgttcgtc caggggatcc ttggggacga
    22861 gcaggatccg gatgtcatgg gccaggaccg cgtccgcttc ttccaggcgc cgcagctcgg
    22921 cctcggccag ggttttcagg tccggatcgg cgcggggatc ggagaggatc tcgcgcgatc
    22981 cggcgatatc cgcccggacg cgcttgagct cctcgatttt cttgacgagc ggctcgatgt
    23041 ccgcccgttc cttggagagg tcgcggaatt tcttagggtc ggagaggacg gacggatcgg
    23101 ccagggcttg aaccagccgg cggtatttgt cctcgacgga ctccagttcg ggaatcatct
    23161 tcccggagcg gcaagcttat ttctttttct tggccgcaag gcccggaccg aatttcttgc
    23221 ggaacttctc gacccggccg gcgctgtcga tgaatttctg ttttccggtg aaaaagggat
    23281 gacattgaga gcagatttcg acccggattt cttttttcgt cgaccgcgtt tggaaggtgt
    23341 tgccgcaggc gcaaatcacc aggcaatcgt cgtaattcgg atgaatattc tgtttcatcg
    23401 aatgatactc cttggaaggc cgtttattat accagacccc ccgaataatt caattcccgt
    23461 cctctctcag tcctcctggg attttctctc gaattcctcc cagtcgacga cgaaccgcgt
    23521 ccggttcatg ttttcccgaa cccggtcgcg gtaggcccgg gattcgaaat gctcgcggac
    23581 gtccttgacc agcgggaaga tctgttcctc ggccttccat tcgtagggcg agccgtccaa
    23641 gcgcttcagg cagttgcgat ggacgatcca ggccatctgc cggatgggaa cgtggctgaa
    23701 cgtccgccgg atcgtctgga cctcggcttc cttggccggg tcggcgaact tgaaataacg
    23761 ggagaacagg acggcgttgt gataataggc cgggaacgcc tgcaggcctt ccttgccggc
    23821 cgtccggccg aggtagatga agatgtccat gatccgcttg ctcatcccca gccccggata
    23881 gagctggccg ggaagcgatc catggcgttc ggaaaaccgg gcttccggat tctggagcgt
    23941 cagccattcg aagacgagcg tcgaaaacgg ccggagcggg gccggggcga gggtcttgcc
    24001 tttcggatcg aagactcctt cccggatttt caagtccacg atcaggcggt ccgggcggtt
    24061 ctcgcggtga aagatctgaa accgctggag cggcggatag gcggaggagt cgagctcgag
    24121 gacgatcggc cacaagccgc gcttccgggc ttccttgtaa aagcccatct tggcc
//
LOCUS       DAOOXH010000032        23164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_2036_length_23164_cov_6.698429, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000032 DAOOXH010000000
VERSION     DAOOXH010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 23164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23164
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2036_length_23164_cov_6.698429"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1521
                     /locus_tag="PKV64_05450"
     CDS             <1..1521
                     /locus_tag="PKV64_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16560.1"
                     /translation="LVIPSVYAEARQMTLPADTLRKISAELASASKSYVASEGGGGLS
                     SLSSVALIAGIKDLKEKKFSDAYARSFLSFKDFVETDYSDNFLASAFKARLLRSSGQT
                     FLHWTLEPERISFARRGDLYVAQFELFLRLEDPSGNLVYQSQEEIPIRLNEEQYKSHE
                     RRRFALQDTLPVIPGKFKLSVLLKNKTGRDFTSVENTVAIAEETGPRLSHLLLSHGQG
                     PVAEAERARLKPFAFNGVQLAFGARPEFTPGETLVCFLQGAGLEAAAGRTVTFGLYGP
                     DGNSVAAADPKPLEDVLGPGGEMEASARFDLSGVKPEYYEARAAVQEADGRILLSAAE
                     PFVLLAQAAPMIPWTLARVRDAFPNAEALTILATQRFTTGGSDRAVPLLERALALKDD
                     PAARLLLAKTLYSLNRYEESIAAAKAVYETGGNREAGKVLALGLTALKDWSGALVYLE
                     QLLEGAIEVEVLNLAAECLLQLGRPGEAVPLLERSLGLVPTQPAVQALLERARKKS"
     gene            1542..2474
                     /locus_tag="PKV64_05455"
     CDS             1542..2474
                     /locus_tag="PKV64_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN16561.1"
                     /translation="MKNKNAAVMVFTLIAAAVFFAPGLSRAQYQGRLEGVVTDTTGAP
                     LAKVTVIVASVKSASMNYTLTTDSQGKFSQVGITPGYYQITFKKEGFQPKSSEIHVRI
                     ADVTRVGATLEKGGEALEKALSDADRLLLKGNKLAEENRYEEAIEAFKEAIALSSFNW
                     GYHFNLGLAYKKINKLEEATAAFQKAVELNPESYSSNKELAEALAKADKFEEAAGHYQ
                     KAVDVSPEDPDAYYNLGACLINVGRTDEAQAAFSKAVELKPDYIDAYYQIGTIAIGQN
                     KIPEAVAALEKFLSLAPDHEKAPVAKQLLEYLKK"
     gene            complement(2524..4155)
                     /locus_tag="PKV64_05460"
     CDS             complement(2524..4155)
                     /locus_tag="PKV64_05460"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HPN16562.1"
                     /translation="MKRREFLNVGLAGSALAAAGVARGSTPDRAGGRGPFSDFPPLKL
                     DETTIAELQEGLRTGAFTSHDLTEAYLRRIEEIDKGGPGLNAVIEVNPDAREIARILD
                     EERAAKGPRGPLHGIPILIKDNIATRDRMATTAGSLALEKAPPSEDAFAVRRLREAGA
                     VLLGKTNLSEWANFRSTRSTSGWSGRGGQTRNPYVLDRNTSGSSSGSGAAAAAGLCAA
                     AVGTETDGSIVSPSSVNGLVGIKPTVGLVSRSGIIPISRTQDTAGPMARTVADAAAVL
                     GALAGADSGDEATREADVRGSRDYLKFLDPEGLRGARIGVIRSFFGFHDRVDALIEAR
                     LEDMKRLGAILVDPVEFPSGRELGSAEFEVMLYEFKAGLNAYLAGLGPEAPVRTLKDL
                     IDYNERRRGLEMPYFGQELFLQAEEKGPLTDAAYLDALKKSLRLSRTEGIDAVMDKHG
                     LDALAAPTDGPAWLTDWINGDHFSGGCSTPAAAAGYPHVTVPAGFVFGLPVGLSFFGR
                     AWSEPVLIRIASGFEQATKARKPPRFLATVDFRNP"
     gene            complement(4478..5581)
                     /locus_tag="PKV64_05465"
     CDS             complement(4478..5581)
                     /locus_tag="PKV64_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16563.1"
                     /translation="MRIVRPAALVLLLPALVLVPAVSSAAAQRKPSAKSMAKSTVQMV
                     PMRTIAGRDQTGGGFGEILDLALDDHRTLYVCDARSADIRKFDDFGTLLGTIGRKGSG
                     SGELDRPVEVEVRGDRVFVRDLGRSRMFVFDLEGRLVSSTPVDEREGRWRKFRALPDG
                     RFVVERETAGETRLELCSAGISPQKTLYRKAVPSNRISAGPLRKDIPLPFAPVVEWDL
                     TPEGLIVAGYSGDYSLEILDPDKGRLAAWTRSFKPFEVTEKDRKDYLAGPGRDLPPAA
                     ARTLVFPKTKPAFEKLVVNGRGRIWTFLPSADPKERTVFEAFDADGTFRSRVILEDEW
                     PRTAPVVFFVGGVWMAVAGPDGETSIVRYQITN"
     gene            complement(6330..8129)
                     /locus_tag="PKV64_05470"
     CDS             complement(6330..8129)
                     /locus_tag="PKV64_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPN16564.1"
                     /translation="MSVSKNAKTTGRTGFTLRTALRELIRTIPDVVYFKDKNRRYLVV
                     NEAFVELCGLTNAEIIGKTDETIFPADLAASFRASDGRVFESGTVFRFEDAVRDPRGR
                     RIVFETIKSPFRSASGNVAGILGISRDITARRRTEEALRESEERFRTLYENATVGLYR
                     TTPEGRILLANPALFRLLGYETFEEIASRDLEREGYGPGYSRAAFKERLERDGFIQGL
                     ETAWKRKDGTTVYVRESARLIRSADGASIFYEGTVEDITERKRAELELRTSEEKYRRI
                     FDRSLEGILQTSLDGRILAANRALVRMFGYASLEEFLSVNMSDLYVDLEARDYMIESL
                     RAGDEVRNLELRMRRKDGAEFYALMNVTALRDPAGRIERIEGMLTDITDLIQSRADLE
                     SALREKEVLLKEIYHRIKNNLQTVSALLGLQGRCFQDPETVRAFAETQDRLRSMAMIH
                     ESLYRSDRLSGVDFPAYVRNLAQALIGAQETSPGRIKLRLDVEPVSFDLKTAVPLGLI
                     INELVSNALKHGFPRDREGTISVELHRRGEDGFHLSIRDDGIGLPPDLELGRLDSMGL
                     CLVEMLTGQIDGRLRLGSAPGANFEITFRAEVS"
     gene            8609..9736
                     /locus_tag="PKV64_05475"
     CDS             8609..9736
                     /locus_tag="PKV64_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPN16565.1"
                     /translation="MYLGREILMFSPLLLYAGVRIGSLLRGTGTRIAWAAVFLLLGGG
                     YPAAEALEHRAAGVPAQVLSTLGYAALPFLLYLVTAVLAVDLLTGLARLGGLLSREKL
                     RRPGSRRVRLGLMLLVPAAFTAFGAWNFNRLEIREYRVVLPRKSAKTERLTIVFAADF
                     HLQARTPRRIMERFADKVNALEPDLVLIGGDIVEGGNTPDEKLEHEAAQFRRLRSAYG
                     VYGVLGNHDSFGGDRSSFFEKAGLRMLRDEYVRIDASIYLAGRLDGRRRTADGGGRKS
                     IDDLLAPVPGDLPIILLDHRPTDLENLGRSRADLQLSGHTHNAQVFPLNFIARKEYEI
                     PWGYGVKGRSQVIVTTGVQGWGPPVRTAGASEIVVVRVTFQ"
     gene            9960..10826
                     /locus_tag="PKV64_05480"
     CDS             9960..10826
                     /locus_tag="PKV64_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPN16566.1"
                     /translation="MDITKRGTIIGILAMVATALLWSIAGLFIKVIDWHPLTIAGFRS
                     LIASFVVLLYVKRPHFHLSFPQVAATVCNAATMMFFVAANKTTTSANAIILQYIAPVC
                     TAFLGAWLLKEKTRLEHWIAIVVVTAGVVVMFMDEIGGGRTLGNVFALLSGLTFSLYF
                     VFMRKQKDESPIESILLSHWLVAAAGLTVTLFVPAPRFTLKAVGAVVVLGVFQIGVAA
                     VLFAAAIKRIPAVTANLIAVIEPVFNPVWVFLALGEAPGAKALAGGAAILIAVTLVSM
                     ISARRPNKPEPA"
     gene            complement(10986..11948)
                     /locus_tag="PKV64_05485"
     CDS             complement(10986..11948)
                     /locus_tag="PKV64_05485"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HPN16567.1"
                     /translation="MDDLMSWKNRYEALFLDISFKKYRMKIDYATIEKAVSRLRGGEA
                     ITYEDLETIAREDLWAFKKYYMWPAREQIEDGLEKTWGLIIDPVARPDKEEDMVRGLL
                     ALFKSLPLASILLRFVWPEHFAIYSRPCLKLLRVERGYDDIEEYFNYNNEMRDYRTSF
                     GLERTADVDMLIWAISQREDEFADIKSLLSEKLPKEFTLLDLIRNSGRRPLKVAEAFF
                     NYGDYQTSGFWASRALEKTLQAACLREHGYLLENTPREKSDIEFLLGQLAGNPVIQKH
                     FKLISSLRNLRNKAVHVGSNFNKQMADEFIDGVGTLAEDLEIIV"
     gene            12128..12316
                     /locus_tag="PKV64_05490"
     CDS             12128..12316
                     /locus_tag="PKV64_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16568.1"
                     /translation="MGRKVYCRHCHSPEVVRAGMAEIRRISGITNKPYGRKKVFLVYK
                     CRACLKYTPCGDLENLVS"
     gene            12750..13151
                     /locus_tag="PKV64_05495"
     CDS             12750..13151
                     /locus_tag="PKV64_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPN16569.1"
                     /translation="MKLTSRSEYALLALVYLARNNGNGFISVETIALAQNIPPKFLEQ
                     ILLVLKRAKYVRSAKGQRGGFKLAKPADGIFLADVIRLFDGALAPTESVSYYFYESTP
                     IEKERRLLQVFRNIRDLVAKKLEGTSIADVL"
     gene            13201..13986
                     /locus_tag="PKV64_05500"
     CDS             13201..13986
                     /locus_tag="PKV64_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPN16570.1"
                     /translation="MIENVTLYGGCGRNGRPEPVSRIDLRMGDIVSIVGPTGSGKTTL
                     INDIELFADDNTPTRRRLLIDGRVPPPEYKENPSSNPIALITQHTTFLSDLPVGEFLS
                     AHARIRSQSDAQTTSLIEETLSFANQLTGEPISPGSRMTELSGGQTRALLIADATVIC
                     DTPIVLLDEVENAGIHRTRALELLRRRRKIFIFVTHDPRIALLSDFRVVMKGGSMVRL
                     LRTDETERSFSAKVDRLDDLLSKMRDRIRMGERLTGAELEGVL"
     gene            13983..14684
                     /locus_tag="PKV64_05505"
     CDS             13983..14684
                     /locus_tag="PKV64_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPN16571.1"
                     /translation="MKLVVCAGPATTGKTSVLRHIVRKLDQCGHRTAFLKIDVQYAEE
                     DALFAAEFGIPVRKVYSGELCPDHCNVMVLGDALLWAGQSTCDVLLVETAGLCLRCSP
                     YVDGGLGLVVLEATSGTNLPLKVGPMLSLADIAVITKIDRVSQAEREVFRARIQDVAP
                     AVRIREVNALHGIGIDPLVDAVLHAPEAPERMFLRGNPPVGTCTICVGKKDVGWQSHF
                     GVVRSLENQNFYRGE"
     gene            14697..15473
                     /locus_tag="PKV64_05510"
     CDS             14697..15473
                     /locus_tag="PKV64_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPN16572.1"
                     /translation="MGKIPSFPNMNNAGGPVLPGLDCGTCGFRTCAELAARLAAEPAL
                     LKRCLYLSEDRFDASSRTQPEAPIDSAAPAAFASPCESRTQAAGSVCPPDRFAVDECA
                     PTYRDSLDREIDFYLEHFPEDPGPREIIIPHNPMITREMDIQPGDILIGRPLGMSCGC
                     PITHCGIACEVDKRTGVIVWCVTGPLDPRSKGFKDLGYYIAEGYEGLVKHARREIRIG
                     ERYFFQPRMCMLQWRHSGLVNFLNRIPGCLQVRLEGLWIG"
     gene            15558..16751
                     /locus_tag="PKV64_05515"
     CDS             15558..16751
                     /locus_tag="PKV64_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPN16573.1"
                     /translation="MPNPGKSIQAVMEAPIPKIKDLARGREDIVSFAQGLPWFGPPPA
                     AVDRVLERIRGGEGTGYSDDLGRPRLRGLLAADLRKQGYAGASPDRLCLTPGANQAVY
                     IALSVLADPGEEVILPRPYYFNNLMALQLLGLKPVLIETGSNGAIDPEAVRRAVSRRT
                     RAIIVISPNNPTGAVAGRETWDRLLQIARQAGIILIADEAYRDFAWDGPHVSPLTMDT
                     ENVIGVFSFSKSYGMAGWRLGFLLAPPELVVQAVKAADTLHICPPAPAQLLAEDILES
                     EPDYPRRFRDDMKASRDRLIAALRPLHARGLISEPRGEGGFYVFIRLTPKHPQSGWEI
                     ARRLADEYALAAVPGEAFGMEGSPWLRLSYGNIRAAEMDSAADRLAEALAEILRSSMS
                     RKGHP"
     gene            17134..18981
                     /locus_tag="PKV64_05520"
     CDS             17134..18981
                     /locus_tag="PKV64_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPN16574.1"
                     /translation="MKNAALIRLPGRFPFVLLAALLSIFPAGCAGRSASTPPVSSVPA
                     ASAEAPLRSMTPPPLVDRNVDILDTELQESIPALIKKARIPGLQAALIRDGRIVLVRG
                     FGVRNRKTQDPVTDETIFEAASLTKPFFAYYVMKLIDQGVLELDKPLLGYIPVEMVEA
                     GLGHPLSEPGFHREWLEKITIRHVLSHSAGTPHGESGKPFPLFFEPGTKWKYSADGYE
                     FLQKIIEHLKGDRIDNLMRREVLEPLGMTRSCLVWKPEYEATMANGHDFFGRPVPYRK
                     RTEAIASATLYTTAEDYAKFVVAVLEGRDLKPETFKEMLTPQIDMDKDKGLGWSLGFG
                     TQRDANGLALWQWGDYGVFRNYVIAYPEKKAGLVYLTNSFLGLSVCSDVVGRVLGGLA
                     TGNLALNYWPYDSPRYQYAWDVRDKGSAAAAELKELSRKNPGLFTLAYIKFLAESLSE
                     AGLAPQSLAVLKAYAAERPQSGSARLELAKAYITMKDCKRARRELDKTRAAAEDKVES
                     AAVDWYLDCVSSIEKPVRLDGEYLKKIAGNYGARHLLYKNGRLSYMRDGRDYPPEGQP
                     LIAISPDTFFMENLLYFRIKVEFDASGNPVKLIGLYVEGLPPDESPRDK"
     gene            complement(19087..19323)
                     /locus_tag="PKV64_05525"
     CDS             complement(19087..19323)
                     /locus_tag="PKV64_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16575.1"
                     /translation="MRLTKKEWEEFEKALAKAIAGDARLTEEEVRAILEAVPDLEIDL
                     ELDETDVDAAIVFGDGGVKNASILYPPKKNAATH"
     gene            complement(19798..21090)
                     /locus_tag="PKV64_05530"
     CDS             complement(19798..21090)
                     /locus_tag="PKV64_05530"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPN16576.1"
                     /translation="MDKESLFMPSRLKVARKIAGLSLEDLSRKIDGRVTRQALSKYEQ
                     GKMSPSPEILNRLEQVLDLKPRYCDTPWAASGAERAGGLKVSGDGGGFAAERDGGSHG
                     YPCGDEEKERRSSLWSGRSNEATIEIRGVSSPAPHGQRVPHSPCAPLPPDAMSYLEFR
                     TSAPLPKKSEEALKHLALEYLARYEDLERLLGRVIVFRNPVAGFSTRTAEEIERAAET
                     VRGHWHLGLSPVPNFLELLESNGIKVFEIRDMDGLGDFDGLAARSAGGYVIALNMNRP
                     PDRIRFTAAHELAHIVCDFDGDGEKERLCHIFAGALLLPRPALERELVHRRRKLTLWE
                     LGAIKRKYGISLQAIMYRACGLGLIAPRHLRDFVAAVKKNGWTIDEPVAYQGREEAIR
                     FKRLLNYAVAERIIGMEQAAALAGTTEAKLQEELGTML"
     gene            complement(21630..23063)
                     /locus_tag="PKV64_05535"
     CDS             complement(21630..23063)
                     /locus_tag="PKV64_05535"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPN16577.1"
                     /translation="MKPFYAKIIMISLLLNAACSFGGHYENRKTVLPSAAGNVRTQIS
                     GKRRNAIPSIGADLQKPEGMSFVGSADPSAPKSRERERSRFYYEKKRMAMQSAVDSVR
                     TRLSGKLEKPIPSISVYIQALNGTWFVSSADLSAQRLTERTHFRFASNTKTFTAAAVL
                     KMHQDGWLDIDSHITAAIPGSSTPYVPDTAGWNIPYKDRITIEQLLQHAAGVYDVDND
                     SRDDYWNGMSYVRWQLSLDPYHQFSADELVGQLVAHPDLYYFEPGTGVPHYSNTGYTI
                     LSEIIARVYSARAGSAKTYSDYLYDHVIGGSSPVPLSVVQFPHRATDAILPAPCVSGI
                     EYAEGGTVPDVYSAQNMSAHVAEGNGYGNFIDLNRFVRTLFSGVNVLHPNTVNTMVNS
                     IGPHAAGSYALGCDHVKNLGYGHNGAIYGYLSTMRYNPETDVSLVVLMPLIDGSDGAT
                     SLGTCIMDGIYAAAWDALKVLGYPGRP"
BASE COUNT         4356 a         7496 c         6823 g         4489 t
ORIGIN      
        1 ctcgtcatcc cctcggttta cgccgaagcc cgccagatga ccctcccggc cgacaccctg
       61 cgcaagattt cggccgagct ggcctccgcc tcgaaaagct acgtcgccag cgaagggggc
      121 ggcggcctgt cctccctgtc ctccgtcgcc ctcatcgccg ggatcaagga cctcaaggag
      181 aagaaattct ccgacgccta cgcccggtcc ttcctcagct tcaaggattt tgtcgagacg
      241 gactattccg acaacttcct ggcgagcgcg ttcaaggccc ggcttctccg gtccagcggc
      301 cagacgttcc tccactggac gctcgagccg gagcggatca gcttcgcccg gcgcggcgat
      361 ctctacgtag cccagttcga gctttttctc cggcttgagg acccatccgg gaatctcgtc
      421 taccagtccc aggaggaaat ccccatccgc ctcaacgaag agcaatacaa gagccacgag
      481 cgccgccgct tcgccctgca ggacaccctg cccgtcattc ccggcaaatt caagctcagc
      541 gttcttctga aaaacaagac cgggcgcgat ttcacctcgg ttgaaaacac ggtcgccatc
      601 gccgaggaaa ccggcccgcg tctcagccat ctcctcctca gccacggcca agggccggtc
      661 gcggaagccg aacgggcccg tctcaagccg ttcgccttca acggcgtcca gcttgccttc
      721 ggcgcccggc cggaattcac cccgggggag acgctggtct gcttcctcca aggagccggc
      781 ctcgaggcgg ccgccggccg gacggtcacg ttcggtcttt acggaccgga cggaaattcc
      841 gtcgccgcgg ccgatccgaa acccctcgag gacgttctcg gccccggcgg cgaaatggag
      901 gcctcggccc gcttcgactt gtccggcgtc aagccggagt actacgaagc ccgggccgcc
      961 gtccaggagg ccgacggccg gattctcctg agcgccgccg agcccttcgt ccttttggcc
     1021 caggcggccc ccatgattcc ctggacgctg gcccgggtgc gggacgcgtt tcccaacgcc
     1081 gaggccctga ccatcctggc cacccagcgc ttcacgaccg gcggatccga tcgggcggtc
     1141 cccctcctgg aacgggccct ggccctcaag gacgacccgg ccgcccggct gctgctggct
     1201 aaaacgcttt actccctgaa ccgctatgag gaatcgatcg ccgccgcgaa ggccgtttac
     1261 gagacgggcg gcaaccgcga ggcgggcaag gtcctggccc tcggcctgac cgccctcaag
     1321 gactggtccg gcgccctcgt ctatttggaa cagctgctgg aaggggcgat cgaggtcgag
     1381 gttctgaacc tggccgctga atgcctgctt cagctcggcc ggcccgggga agccgttccc
     1441 cttctcgagc gctccctggg gctcgtcccc acgcagccgg ccgtccaagc cctcctcgag
     1501 cgggccagga aaaaatcgtg attcctcgga aggagacacc gatgaaaaat aaaaacgccg
     1561 ccgtgatggt tttcaccttg atcgccgccg cggttttctt cgctcccggc ctgtcccggg
     1621 cgcaatacca ggggcgcctc gagggcgtcg tcacggatac cacgggagcg ccgctggcca
     1681 aggtcacggt gatcgtcgcc tccgtgaagt ccgcctccat gaactacacc ctgacgaccg
     1741 actcccaggg caagttcagc caggtcggaa taacgccggg ctactaccag atcaccttca
     1801 agaaggaagg cttccagccc aaatcctccg agatccacgt ccggatcgcc gacgtcaccc
     1861 gggtcggggc gacgctcgag aagggcgggg aggcgctgga aaaagctctt tccgacgccg
     1921 accggcttct gctcaagggg aacaagctcg ccgaggaaaa ccggtatgaa gaggcgatcg
     1981 aggccttcaa ggaagccatc gccctcagct cgttcaactg gggataccat ttcaacctcg
     2041 gcttagccta taaaaagatc aacaagctcg aggaagcgac ggccgccttc caaaaggccg
     2101 tcgagctcaa tcccgaaagc tacagctcca acaaagagct ggccgaggcc ttggccaagg
     2161 ccgacaagtt cgaggaggcg gcgggccatt accagaaagc ggtggacgtc agccccgagg
     2221 atcccgatgc ctactataac ctgggggcct gcttgatcaa cgtgggccgg acggacgaag
     2281 ctcaggccgc cttttcaaaa gccgtggagc tcaagccgga ttacatcgac gcctattatc
     2341 agatcgggac gatcgccatc ggccaaaaca agattcccga ggccgtcgcc gctctggaaa
     2401 aattcctctc gctggccccc gatcacgaga aggcgccggt ggccaagcag ttgctcgaat
     2461 atcttaaaaa ataacgccgg ccgcgacgac ggcggcggcc gaggggcggc cggcgggctc
     2521 ccctcaggga ttacggaaat caaccgtggc cagaaagcgg ggcggcttgc gggctttggt
     2581 cgcctgctca aacccggagg cgatcctgat caagaccggc tcgctccaag cccggccgaa
     2641 gaaggacagg ccgacgggaa gaccgaagac gaacccggcc gggacggtga cgtgcggata
     2701 cccggccgcg gccgccggcg tcgagcatcc gccggagaaa tggtcgccgt tgatccaatc
     2761 cgtcagccag gcggggccgt ccgtgggcgc ggccagggcg tcaaggccgt gcttgtccat
     2821 gaccgcgtcg atcccctccg tccgcgacag gcggagcgat tttttcaagg cgtcgaggta
     2881 agccgcgtcg gtcagggggc ctttctcttc cgcctgaagg aaaagctcct ggccgaaata
     2941 aggcatctcc aggccgcggc ggcgctcgtt gtaatcgatg agatccttga gcgtgcggac
     3001 gggggcctcg ggcccgagcc cggccaagta ggcgttgagg ccggccttga actcgtacaa
     3061 catgacttcg aattcggccg agccgagctc ccggccggag ggaaattcca ccgggtcgac
     3121 gaggatcgcc ccgagccgct tcatatcctc gagccgggcc tcgatgaggg cgtccacccg
     3181 gtcgtggaag ccgaagaacg agcggatcac gccgatccgg gcgccccgga ggccctccgg
     3241 atcgagaaat ttcagataat cgcgcgaacc gcggacgtcc gcttcccggg tcgcctcgtc
     3301 tccggaatcg gccccggcca gggctcccaa aacggccgcg gcgtcggcca cggtccgggc
     3361 catcgggccg gccgtatcct gggtgcggga gatggggatg attccggacc ggctgaccag
     3421 cccgaccgtc ggcttgatcc cgacgaggcc gttgaccgac gaggggctga cgatcgagcc
     3481 gtcggtttcc gttccgacgg ccgcggcgca gaggccggcg gccgcggccg cccccgagcc
     3541 ggagctcgat cccgacgtgt ttcggtccag gacatacgga ttgcgggttt ggccgccccg
     3601 gccgctccag ccgctggtcg agcgggtcga ccggaaatta gcccattcgc tgaggttcgt
     3661 cttgccgagg aggacggccc cggcctctcg gagcctccgg acggcgaacg catcctccga
     3721 agggggcgcc ttctccaggg cgagcgatcc ggccgtcgtg gccatgcggt cgcgggtggc
     3781 gatgttgtcc ttgatgagaa tcggaatccc gtggaggggg ccgcgcggcc ccttggccgc
     3841 ccgttcctca tccaggatcc gggcgatttc ccgggcatcc ggattgacct cgatgaccgc
     3901 gttgagcccg ggtcctcctt tgtcgatctc ctcgatccgg cggagataag cttcggtcag
     3961 gtcgtgcgag gtgaacgctc cggtccgcaa tccctcctgg agctcggcga tcgtcgtctc
     4021 gtcgagcttc aacggcggaa aatccgaaaa cggcccccgc ccgcccgccc ggtccggagt
     4081 cgagccgcgg gcgaccccgg ccgccgccaa ggcgcttccc gccaagccga cgttcaagaa
     4141 ttcccttcgt ttcatctccg tcctcctttt tcgtcattcg gccgcgttcg gacgggggac
     4201 gtccggccgg ccgttttttc cactcgccgt cgtcgagaga aacccgattc gccttcccct
     4261 tcgccctttc ggcttcgccg tcccgcgcgg cgggccaaac cgcccggagc cgggacggcc
     4321 gcccgacgcc cgaagcgccg tctcgtcccc gcctcccctt ccgtcctcgg cgccgcttaa
     4381 ggcggcgggc cgcggccggc gaccggcccc cggagtgtct ctcggcgagc ggctttctcc
     4441 tccggccaaa agaggccggg gccgcccgcc ccgcccgtca gttggtgatt tgataccgga
     4501 cgatggatgt ctccccgtcc ggtccggcga cggccatcca aactccgccg acgaaaaaaa
     4561 ccacgggcgc cgtccgcggc cattcgtcct cgagaatcac ccggctgcgg aacgtcccgt
     4621 cggcgtcgaa cgcctcgaag acggtccgtt ccttcggatc ggccgaggga aggaacgtcc
     4681 agatgcgtcc ccggccgttg accacgagct tctcgaaggc gggcttcgtc ttcgggaaga
     4741 cgagggttcg ggcggccgcg ggcggcaggt cccggcccgg cccggcgaga tagtccttcc
     4801 ggtccttttc ggtgacctca aaaggcttga aggaccgcgt ccaggcggcg aggcggccct
     4861 tgtcgggatc gaggatctcg agggaataat ccccggaata tccggcgacg atcagccctt
     4921 ccggcgtcaa gtcccactcc acgacgggag cgaagggaag gggaatgtcc ttgcggagcg
     4981 gtccggcgct gatccggttg ctcggaacgg ctttccggta aagggttttt tgcggggaaa
     5041 tgccggccga gcaaagctcg agccgcgtct cccccgccgt ctccctctcg acgacgaacc
     5101 gcccgtcggg cagggcccgg aactttctcc agcggccttc ccgttcgtcg acgggcgtcg
     5161 aggacacgag ccggccttcg aggtcaaaga cgaacatccg cgaccgcccg aggtcgcgga
     5221 cgaacacccg gtctccgcgc acttcgacct cgacgggccg gtcgagttcc ccggatcccg
     5281 aacccttccg gccgatcgtc ccgagcagcg ttccgaaatc atcgaatttc cggatgtcgg
     5341 cggaccgggc gtcgcagacg taaagcgtcc ggtggtcgtc caaagccaag tcgaggattt
     5401 cgccgaaacc gccgccggtt tgatcccggc ccgcaatcgt ccgcatcggg accatttgga
     5461 cggtgctctt ggccatgctc ttggcggagg gctttctctg tgcggcggcg gaggaaaccg
     5521 ccgggaccaa aaccagggcc ggaagaagaa gaacgagagc cgcggggcgg acgattcgca
     5581 tcggtgaagc tccttcggac ttccccgtcg ggaaggggtt tattttaacc caaagaatgg
     5641 agtccctcaa ggaatccgcg gggcgggaag caagccggtt cgggaaaaag ggggaagccc
     5701 gccgggcgcg gatataacga tgattcttat gctctgattc atcgccggct tatcgccatc
     5761 gatgcctgcg gaggtccatg cccgcggaac ttggcaaaag gggggccgtc gtgaatctcc
     5821 cgggcaggcg ataggacgcg tcaactcata aaacgtttcc aaggatccga tcgtcttgtc
     5881 ctcccggata tcgcttcgct cgccgctccc gtcctccgcc tattcggctg gacggcggat
     5941 cgtcgtccgg acgcgcgatt tgacggaggg aaaaacgccg gcctcccggc ggcgtcggcg
     6001 gatctcccgg ttcagccgcc cccggccgtt ggtcgaagag atccgtttta gagatcgacg
     6061 gccggtccct cttcccggcc ccggatcgac tcggggaagc gcttttcatg gccgtcgatc
     6121 cgggccgcgg ccgccccgcc gggccgatcg aaaataccgt caagatccgg acgctcaagc
     6181 ggaggccgtc ccgtcccggc tcctttgatc cgcaggaacg ggcggagacg gattcgcttg
     6241 aagtccgcga gcccgatccg tcgaatcccg gaacaaatcg ggcgggggcg aaggcggatc
     6301 ccggcggcgg ctgaagggga aggatgacgt caggaaacct cggctcggaa cgtgatctcg
     6361 aaattcgctc cgggcgcaga gccgagccgc aagcggccgt cgatttggcc ggtgagcatc
     6421 tcgacgaggc aaaggcccat cgaatccaac cggccaagct ccaagtccgg cggcagtccg
     6481 atgccgtcgt cccggataga cagatggaat ccgtcttccc cccggcgatg aagctcgacc
     6541 gagatcgtcc cttcccggtc ccggggaaaa ccgtgcttga gagcgttgga cacgagctcg
     6601 ttgatgatca ggcccagggg gacggccgtt ttaagatcga agctcaccgg ttcgacgtcc
     6661 aggcgcagct tgatccggcc gggagaggtc tcttgcgcgc cgatcaatgc ttgggccagg
     6721 ttccggacgt aggcggggaa atcgacgccg gacaagcggt ccgagcgata gaggctctcg
     6781 tggatcatgg ccatcgagcg gagccggtcc tgggtttccg cgaaggcccg gacggtctcg
     6841 gggtcttgaa aacacctccc ttgaagaccg agcagcgccg agacggtttg caggttgttc
     6901 ttgatccggt gatagatctc cttcaggaga acttccttct cccgaagggc ggactcaagg
     6961 tccgcccgcg attggatgag atcggtgatg tcggtcaaca tgccttcgat ccgctcgatc
     7021 cggccggccg ggtcgcgcag ggccgtgacg ttcatcaagg catagaactc cgcgccgtcc
     7081 ttccgccgca tccggagctc caggttccgg acctcatcgc cggcccggag gctctcgatc
     7141 atgtaatcgc gcgcctcgag atcaacgtag aggtcggaca tgttgaccga taaaaattcc
     7201 tcgagcgacg cgtagccgaa catgcggacc aaggcccggt tggccgcgag gatccggccg
     7261 tcgagcgacg tctggagaat gccctccagg ctgcggtcga agatccggcg gtatttttcc
     7321 tcggaagtcc ggagctcgag ctcggcccgc ttccgctcgg tgatatcctc gaccgtgccc
     7381 tcgtaaaaaa tcgacgcccc gtccgcgctc cggatcagcc gggcgctttc ccggacataa
     7441 accgtcgtcc cgtcttttcg cttccacgcc gtctccaaac cctggatgaa gccgtcgcgc
     7501 tcgaggcgtt ccttgaacgc ggcccgggaa tatcccggcc cgtatccttc ccgctccaag
     7561 tcccgggaag cgatctcctc gaatgtttcg tatccgagga ggcggaacag ggcgggattg
     7621 gccaggagga tccggccctc gggcgtcgtc cggtagaggc cgaccgtcgc gttctcgtac
     7681 agcgtccgga aacgctcctc gctttcccgc agggcttcct ccgtccgccg cctcgcggtg
     7741 atgtcccggc tgatgccgag gattccggcg acgtttccgg acgcggatcg aaacggggac
     7801 ttgatcgtct caaagacgat ccggcggccc ctcggatccc tcaccgcatc ctcgaaccgg
     7861 aaaaccgtcc ccgattcgaa gacgcggccg tcgctggccc ggaacgaggc ggccaggtcg
     7921 gccggaaaga tcgtctcgtc ggtttttccg atgatctccg cgttcgtcaa tccgcacagc
     7981 tccacaaagg cctcgttgac aactaaataa cgccggtttt tgtccttgaa atagacgacg
     8041 tccgggatgg tccggatcag ctcccggagg gcggtccgga gggtgaagcc agttcggccc
     8101 gtcgttttcg cgttcttgct gacggacatg aacaacctct cttcgcgggt cgcattggac
     8161 caggggaaaa aagccgatat aaaaaggaat tatagaaaaa caaaatcccc cgaaacaagg
     8221 ccggggaggg gtgaaatatt tcgaagcggg gggttaatcc cccccatgcc tgatccggat
     8281 cagagacatg gtggacgcct ctaaaacgac agccgggcgg ttaaagggga tcgatttcca
     8341 ccgtcggggc ggaaatatcc gtccgacctg tcgccgcgtt catatctccc cgacgacttt
     8401 ccactagggg ctggggcggt tcgattccga ggatctaacg acgttggagg gcgagtcgac
     8461 gcggccccat caggctcggg gcgatccgac tccctatgaa ccgggccgcc ggaaaaacca
     8521 attccccaag ccccgggagg tgtccacgat gtcgtgaccc aggacaatgc cttcccccgg
     8581 cgccgtctcc cgggtataat gccgccgcat gtacctcggc cgcgaaatcc ttatgttctc
     8641 gccgcttctt ctctacgccg gcgtccggat cggaagcctc ctccgcggga ccgggacgcg
     8701 gatcgcctgg gcggcggtgt ttcttctgct gggcggcggc taccccgcgg ccgaagccct
     8761 ggaacaccgg gcggccggcg tcccggccca ggtactctcg accctcggct acgccgccct
     8821 cccctttctc ctctacctgg tgacggccgt cctggccgtg gaccttctca cggggctggc
     8881 ccgcctcggc ggcctcctgt cccgggagaa gctccggcgg ccgggaagcc ggcgcgtccg
     8941 cctgggcttg atgctgcttg ttccggccgc gttcacggcc ttcggcgctt ggaacttcaa
     9001 ccgcctggag atccgggaat accgggtcgt tcttccccgg aaatcggcca agaccgagcg
     9061 cttgacgatc gtcttcgcgg ccgacttcca ccttcaggcg cggacgcccc ggcggatcat
     9121 ggagcggttc gccgacaagg tcaacgccct cgagccggac ctcgtcctca tcggcgggga
     9181 tatcgtcgaa ggcggcaaca cgcccgatga aaagctggag cacgaagccg cccagttccg
     9241 gcgccttcgg tcggcctacg gcgtttacgg cgtcctgggg aatcacgatt ccttcggcgg
     9301 cgaccggagc tcgtttttcg aaaaggccgg ccttcgcatg cttcgcgacg agtacgtccg
     9361 gatcgacgcc tcgatctacc tggccgggag attggacggc cgccggcgga cggcggacgg
     9421 agggggccgg aaatccatcg acgacctcct cgctccggtg cccggggatt tgccgatcat
     9481 ccttctggat caccggccga ccgacctgga aaacctgggc cggagccggg cggaccttca
     9541 gttgtccggc cacacccaca acgcccaggt cttccccttg aatttcatcg cccggaagga
     9601 atacgaaatc ccgtggggat acggagtcaa gggccgctcc caggtcatcg tcacaaccgg
     9661 cgtccagggc tgggggccgc ccgtccggac ggccggcgcc tcggagatcg tcgtcgtccg
     9721 ggtgacgttc caataataat tcggaacctg tttatccatt tccggatttc cggtctcttc
     9781 atatccgtac ctggttatcc atttccgcat ttcgtatccg tgcctggtcc tctgaaatca
     9841 gtacctgttt atccatttcc gactctcttt cgaggttgac tcggagtgcg agcggacata
     9901 tcatccatcg aaaaagatct tcacggaatg gaaatgatcg cggtgagaga atcgagacga
     9961 tggacataac gaagcgcgga acgatcatcg gaatcctggc gatggtcgcc acggctttgc
    10021 tttggagcat cgccggctta tttatcaagg tcatcgactg gcaccccctg acgatcgccg
    10081 gctttcgcag tttgatcgcc tcgtttgtcg tcctcctgta cgtgaagcgg ccgcatttcc
    10141 acctgtcttt cccccaagtc gcggcaaccg tctgcaacgc cgcaacgatg atgttctttg
    10201 tcgcggccaa taagaccacc acctcagcta acgccatcat ccttcagtac atcgcccccg
    10261 tctgcacggc cttcctcgga gcctggctgc tgaaggagaa aacccggctg gagcactgga
    10321 tcgccatcgt cgtggtgacg gccggcgttg tcgtgatgtt catggacgag atcgggggcg
    10381 ggcggacgct cggtaacgtc ttcgcccttc tctccgggct caccttcagc ctctacttcg
    10441 tcttcatgcg caagcagaag gacgaatccc cgatcgaatc catcctgctc tcccactggc
    10501 tggtggcggc cgcggggcta acggtcacgc tgtttgtccc ggccccgcgc ttcaccttga
    10561 aagcggttgg ggcggtggtt gttctggggg ttttccagat cggggtggcg gccgtcctgt
    10621 tcgccgcggc gatcaagcgc atcccggccg tcacggccaa cctgatcgcg gtcatcgagc
    10681 cggtcttcaa tccggtctgg gttttcctgg ctttaggcga ggcgcccggc gccaaggctc
    10741 tcgccggagg ggcggcgatc ctcatcgccg taacccttgt ctcgatgatc agcgcccgcc
    10801 ggcccaataa accggaacct gcttaaccat ttccgcattt cgtctttatc cgaacctggt
    10861 tatccatttc cggatttcaa gaaatgattt caagaaatgg agaaaaggat aagcaggttc
    10921 cgatttaacg ggaaatgggg aaaaggttga acaggtaccg attcggtcga aacaacgctg
    10981 actcgtcaga cgataatttc cagatcctcg gccagggtgc cgacgccgtc gatgaactcg
    11041 tcggccatct gcttattgaa gttgcttccg acgtggacgg ccttgttccg gaggttccgg
    11101 agcgaggaaa tcagcttgaa atgcttttga atgacgggat tgccggcgag ctgccccagt
    11161 aggaattcaa tgtcgctttt ttcgcgcggc gtattttcca aaaggtagcc gtgttcccgg
    11221 agacaggcgg cctggagggt tttctccagg gcccgcgaag cccagaaacc cgacgtctga
    11281 taatccccgt aattgaaaaa agcctcggca accttgagcg gccggcggcc tgaattccgg
    11341 atcagatcca gcagcgtgaa ctccttgggg agcttttcgc tcagcagcga cttaatgtcc
    11401 gcgaactcgt cctcccgctg ggaaatcgcc cagatcagca tgtcgacatc ggccgtccgc
    11461 tcgagcccga acgatgtccg gtagtcgcgc atctcgttgt tgtagttgaa gtactcctcg
    11521 atatcgtcgt agccgcgctc cacccgaagg agcttcaggc agggccggga ataaatcgcg
    11581 aaatgctccg gccagacaaa ccgcaggagg attgaggcca gggggaggct cttgaacagg
    11641 gccaggagcc cgcggaccat atcctcttct ttgtccgggc gggccaccgg atcgatgatc
    11701 agcccccacg ttttctccag gccgtcctcg atctgttccc gggccggcca catgtaatac
    11761 ttcttgaacg cccagaggtc ttcccgggcg atcgtctcca ggtcctcgta tgtgatggct
    11821 tcgcctcccc ggagacggct gaccgctttt tcgatggtcg cgtaatcgat cttcatccgg
    11881 tatttcttaa aggagatgtc caggaagagg gcttcatacc ggtttttcca gctcataagg
    11941 tcatccatct caaaacctct ttttctcccg aggaggcatt tcccccgccg aaattcatta
    12001 tttcacgatg tctcgctcga ctcaagccct tgaattcacg aaaattccct cgaaaatgag
    12061 agctgggccg gagggatcga agtacgatga tccggccccc cggaacgccg gggggtataa
    12121 tgaatttatg ggccggaaag tctactgccg ccactgccat agccccgaag tcgtccgggc
    12181 cggcatggcg gaaatccggc ggatctcggg aatcacgaac aagccctatg gccgcaaaaa
    12241 agtctttctc gtctacaagt gccgcgcctg cctgaagtac acgccctgcg gcgacctgga
    12301 aaacctcgtc tcttgagatc cgccgcgccc cgcgctccgg atgtccgcaa cgccggcccc
    12361 ttcccttccc cgttcattcc gaacctggtc aaccatttcc tcaatacaac cttttctctg
    12421 catcggcacc tgacgaatca gcaccttcct tggaatcggt acctggtcaa ccatttcccg
    12481 aattctccgc ggcaggcctt tccaggggaa ttcctggcga gaaatgtgga aatggaaaat
    12541 ggaggacctg gtaccgattt gtcgatttgt atcgattgac tgtgccgatt taccgatttt
    12601 aagatgccga tttcgggacc gattttttta cggcttcgcc cggcgcctct cttgaaggca
    12661 gaacatcccc aggcagatga aaaaattccg cttgacaaga aaagagcttt tatattacaa
    12721 ggcctattgt ataagtcatt aaaacaaccg tgaaacttac ttcgcgcagt gaatacgctc
    12781 tcctggccct tgtttatctg gctcgaaaca atgggaatgg attcatctcg gttgaaacaa
    12841 tcgccctggc ccagaacatc ccgccgaaat ttttagagca aattctcctc gtccttaagc
    12901 gggccaagta cgtgcggagc gcgaaaggcc agcggggagg attcaagctg gccaaacccg
    12961 cggacgggat ctttctggcc gacgtcattc gtcttttcga cggggccttg gcccccaccg
    13021 aatccgtcag ctactacttt tatgaatcga cgccgataga aaaggaaagg cgccttcttc
    13081 aggtgttccg caacatcagg gacctcgtgg cgaaaaagct cgaagggaca tcgatcgccg
    13141 acgttctttg atttttttta gaagtttata tgacttatct aataggctaa ggagtaaaag
    13201 atgatcgaga acgtgactct ttacggagga tgcggccgaa acggccgccc ggaacctgtc
    13261 tctcggatcg accttcggat gggcgacatc gtcagcatcg tcggaccgac cggctcaggc
    13321 aaaacgaccc tgatcaacga catagagctt ttcgccgacg acaacacgcc gacccggcgc
    13381 cgtctcctta tcgacggccg cgtccccccg cccgagtaca aggaaaaccc ctcctccaac
    13441 ccaattgccc ttatcaccca gcacacgact tttctttccg atctgccggt gggcgaattc
    13501 ctctcggccc atgcccgaat ccgaagccag tcggacgccc agacgacgag cctgatcgaa
    13561 gagacgttat cgttcgccaa tcaactcacg ggagaaccga tctccccggg aagccggatg
    13621 accgagcttt ccggcggcca aacccgggcc ctcctcatcg ccgacgcgac cgtcatctgc
    13681 gacacgccga ttgttctcct ggacgaagtc gaaaacgcgg ggattcacag gacccgagcc
    13741 ttggaactcc ttcgccggcg ccggaagatc ttcatcttcg tcacccatga tccgcgcatc
    13801 gctcttcttt ccgactttcg agtggttatg aagggcgggt ccatggtccg ccttctccga
    13861 accgatgaga ccgagcggag cttttcggcc aaggtcgacc gtctcgacga cctcctctcc
    13921 aagatgcggg accgtatccg gatgggcgag cgcctgaccg gagcggagct ggaaggtgtg
    13981 ctgtgaagct cgtcgtatgc gccggcccgg cgacaacagg aaaaacttca gtcctccggc
    14041 acattgtccg caaactcgac caatgcgggc atcggactgc ctttctcaaa atcgacgttc
    14101 agtatgccga ggaagatgcc ctctttgccg ccgaattcgg aatccccgtc cgcaaggtgt
    14161 attcgggcga gctctgtccg gaccattgca atgtcatggt cctgggcgac gccctgctct
    14221 gggccggaca atcaacatgc gacgttctcc tggttgaaac ggccgggctc tgcctccgct
    14281 gctcgcccta cgtcgacggc gggctggggc tcgtcgttct cgaagccacc agcgggacga
    14341 atcttcctct caaagtcggg ccgatgctgt cgctggccga catcgccgtc ataacgaaga
    14401 tcgaccgcgt ttcccaggcc gagcgcgagg tgtttcgggc ccggatccag gacgtcgccc
    14461 cggccgtccg catccgcgaa gtcaacgccc ttcatgggat cggcatcgat ccgctcgtcg
    14521 acgcggttct gcacgccccc gaagctccgg agcggatgtt cctgcgaggc aatccgccgg
    14581 tcgggacctg cacaatctgc gtcggcaaaa aggacgtcgg ctggcaatct catttcggcg
    14641 tcgtccgttc gctcgaaaac cagaatttct accgcggaga ataaggagat cacatcatgg
    14701 gcaaaatccc gtcattccct aatatgaata acgccggcgg ccctgtcctt cccggcctcg
    14761 actgcgggac ctgcggcttt cggacgtgcg ccgagctggc cgcccggctg gccgcggagc
    14821 cggccctcct caagcgctgc ctttatttgt ccgaagaccg cttcgatgcg tcctcccgga
    14881 cgcagcctga agcacccata gattcagccg cgccggcggc ttttgcttcg ccttgcgaaa
    14941 gccggactca ggccgccggc agcgtctgtc cgccggaccg gtttgcggtt gatgaatgcg
    15001 ccccgacata ccgcgactct ctcgaccggg aaatcgactt ctatctcgaa cattttcccg
    15061 aggatcccgg accgcgggag atcatcatcc cccataatcc catgatcacc cgggaaatgg
    15121 acatccaacc gggcgacatt ctcatcggcc ggccgctcgg aatgtcctgc ggctgtccca
    15181 tcactcactg cggaatcgcc tgcgaagtcg acaagcggac gggcgtcatc gtctggtgcg
    15241 tgaccggccc tctcgatccg cggagcaaag gcttcaagga tctcgggtat tacatcgccg
    15301 aaggctatga aggactggtc aagcacgccc gccgggaaat ccgcatcggc gaacggtatt
    15361 tttttcagcc gcgcatgtgc atgcttcagt ggagacacag cggactggtc aacttcctca
    15421 accggatccc gggttgtctc caggtccggc ttgaagggct ctggatcggt tgacgctccg
    15481 ccgttccggt ctttgacaag gaatggcggg aacgttacga tgcccctgcg ccgccgggca
    15541 ggcggcggga ggacatgatg ccgaatccgg gaaaatccat ccaagccgta atggaagcgc
    15601 cgatcccgaa gattaaggat ctggcccgcg gccgggagga catcgtttcg tttgcccagg
    15661 gcctgccctg gttcggcccg ccgccggccg ccgtggatcg cgttctggaa cggatccgcg
    15721 gcggcgaggg caccggctat agcgacgatc tcggccgccc gcgcctgcgc ggccttctgg
    15781 ccgctgacct gcggaagcaa ggctatgccg gggcttcgcc cgatcgcctt tgcttgacgc
    15841 ccggggccaa tcaggccgtt tatatcgcgc tttcggtcct ggctgatcca ggcgaagaag
    15901 taatcctccc gcgaccatat tatttcaaca acctgatggc cctccaactg ctcggcttga
    15961 aacccgtgct catcgaaacg ggatcgaacg gggcgatcga tccggaggcc gtccgccggg
    16021 ccgtcagccg ccgaacccgg gcgataatcg ttatttcgcc gaacaatccc acaggcgccg
    16081 tggccggccg ggagacatgg gaccggcttc tccagatcgc ccgacaggcc ggcatcatcc
    16141 tgatcgcgga cgaggcatat cgcgatttcg cctgggatgg cccgcacgtt tcgccgttga
    16201 cgatggacac ggaaaacgtc atcggcgttt tttctttttc caaatcgtac ggcatggccg
    16261 gttggcgtct gggtttcctc cttgccccgc cggaattggt cgtccaggcc gtcaaggccg
    16321 ccgacacgct gcatatctgc ccgccggcgc cggcccagct cctggctgaa gacattctcg
    16381 aatccgagcc ggattatccg cggcgattcc gcgatgacat gaaagcctcc cgcgaccgtc
    16441 tgatcgccgc gctgcggccc cttcatgccc ggggattgat cagcgaaccg cgcggagagg
    16501 gtggattcta cgttttcatc cggttaactc ccaaacaccc tcaaagcggc tgggagatcg
    16561 cccggcggct ggcggacgaa tatgccctgg ccgcggttcc cggcgaagcc ttcggcatgg
    16621 aaggaagccc ctggctgcgc ctcagctacg gaaacatccg ggcggcggaa atggattcgg
    16681 cggcggaccg gctggccgaa gccctggccg aaatccttcg ctcctccatg tctcgaaagg
    16741 gacatccttg agatcgacgg gctcccggcc gggatcattc tccgtctgat ctcaaaacgc
    16801 ccggacgaag ggcggcccga ttacaacctt gtctcccgga ttactgatgc gtccggggac
    16861 ggaccgcgcg ccgggtttga tgacgggcgg gacctcggat gccgttgccg ggaattcgcg
    16921 ccggaggatg gatcgagatg cggagtgtcc ttatatgaaa gacaatcggc gtctctgaca
    16981 ttcggaacct ggtcaaccat ttcccgaatt ctcgggcata ggcctttcca ggggaattca
    17041 agcggggaaa tacggaaatg gttaaccagg ttcggattta ttttaacctt ttcgccgttt
    17101 tcagcgtatt aacgggaaag ggagatatgc atcatgaaaa acgccgctct gatccgtctt
    17161 ccaggccgct ttccgtttgt cctcctcgcc gcgctccttt ccatctttcc ggcgggttgc
    17221 gccggccgat ccgcttcaac gccccctgtt tcttccgttc ccgccgcctc ggccgaggcc
    17281 cccctccggt ccatgacccc gccgccgttg gtcgaccgca atgtcgacat tctcgacacc
    17341 gagctccaag aatcgattcc ggccctgata aagaaagccc ggatccccgg actccaagcg
    17401 gctctcattc gcgacggccg gatcgtcctc gtccgcggct tcggagtccg caacaggaag
    17461 actcaggatc ccgtcactga cgaaacgatt ttcgaggccg cctccctcac caagcccttc
    17521 ttcgcttatt acgtgatgaa gctcatcgac cagggcgtcc tcgagctcga caaaccgctg
    17581 ctcggctata tccccgtcga gatggtcgag gccggattgg gccaccccct ctccgagccg
    17641 ggattccacc gcgaatggct ggaaaagatc acgatccgcc acgtcctcag ccactcggcc
    17701 ggcaccccgc acggcgagag cggaaagcct tttccgcttt tctttgagcc cgggacgaaa
    17761 tggaaatact cggccgacgg ctatgaattc ctgcaaaaaa tcatcgagca tctcaaggga
    17821 gaccggatcg acaacctgat gcggagggaa gtcctcgagc ccctcggcat gacccgaagc
    17881 tgcctggtct ggaaacccga gtatgaagcg acgatggcca acggacacga ttttttcggc
    17941 cggccggtcc cctaccgaaa gaggacggaa gccatcgcct cggccaccct ttacacgacg
    18001 gccgaggatt acgccaagtt cgtcgtcgcc gtcctcgaag gccgggatct caagcccgaa
    18061 acattcaagg aaatgctgac tccgcagatc gacatggaca aggacaaggg cctcggctgg
    18121 agcctgggct tcggcacgca gcgcgacgcc aacggcttag ccctctggca atggggggat
    18181 tacggcgtat tccggaatta cgtcatcgca taccctgaaa agaaagccgg tcttgtttac
    18241 ttgaccaaca gttttctggg gctgagcgtc tgttccgacg tggtcggccg cgtcctgggc
    18301 gggctggcca cgggaaatct cgctctgaat tattggccgt acgattcgcc gcgctatcaa
    18361 tatgcctggg acgtgcggga caagggttcg gcggccgccg cggagctgaa agagctgtcg
    18421 cgaaaaaatc ccggattgtt cacgctcgct tacatcaaat tcctggccga atccctctct
    18481 gaggccggcc tggcccccca gtccctggcc gtcttgaaag cttacgcggc cgaacgtccg
    18541 caatcaggct ccgcccggct cgagctggcc aaggcgtata taacgatgaa agactgcaaa
    18601 cgagcccggc gggaattgga caaaacccgc gccgccgccg aggacaaggt tgaatccgcg
    18661 gccgtcgatt ggtatctcga ctgcgtctcc tccatcgaaa agccggtccg gctggacggg
    18721 gagtatttga agaagatcgc tggaaattac ggcgcgcggc atcttcttta caagaacggc
    18781 cgcctctcct acatgcgcga cggccgggat tacccgccgg aaggacaacc gttgatcgcc
    18841 atatcccccg acaccttctt catggaaaac ctgctctatt tccggatcaa ggtcgagttc
    18901 gatgcatccg gtaatccagt caagttgatc ggtctctacg ttgaaggctt gccgccggac
    18961 gaatcgcccc gggataaata aaccggtacc tggtccacca tttcccgaat tctccgcggc
    19021 gggcttttcc agggggattc cggcggggaa atgcggaaat ggaaaaccag gttcggattt
    19081 gtctcctcag tgcgtcgccg cgttcttttt cggcgggtaa agaatcgagg cgttcttcac
    19141 cccgccgtcg ccgaagacga tggccgcgtc gacatccgtc tcgtcaagct caagatcgat
    19201 ttccaaatcc ggaacggctt ccaaaatcgc ccgcacctct tcttccgtga ggcgggcgtc
    19261 gccggcgatc gccttggcca gcgctttctc gaattcttcc cattccttct tggtcaatct
    19321 cacgctctcc ttgccgcgtc gatgggcccg aggccgtctc cggcccccct tatattataa
    19381 ccccgccgcc gtcttaaggt tgcttggcat tcgaggcgcc ggcccgtcgc cgccgggtcg
    19441 ccgatttccc tcatttccgc caacgctatt catggagcta ttcgcgacct tctcatcttc
    19501 tgctccggcg gaaaaacgtt ggatacgcga agagcccatc cgcggacggg ccggcccgac
    19561 gatcttaaat ccgttttgaa gcggtgatct gtgctcgggc cgggggaatc ctatgccttc
    19621 ttgccgcaag ccgccgcctt tctcctgatt cctcttacag ccgccgtccc cgccgccacc
    19681 cccagactcc ctcagacttc cccctccggc ttcaaggggc cgccgccgcc ttgccgcggc
    19741 tcattcctcc atgcggcggc cccttcgatc cccctcagcc cccacctttg gaaatattca
    19801 aagcatcgtt ccaagctctt cttgaagctt cgcctccgtc gtcccggcca gcgccgcggc
    19861 ctgctccatt ccgatgatcc gctccgcgac cgcataattg aggaggcgct tgaagcggat
    19921 cgcctcctcc ctcccctgat aggccacggg ctcgtcgatc gtccagccgt tttttttgac
    19981 ggcggccacg aaatcgcgca ggtggcgcgg cgcgatcaag cccaagccgc aagcccggta
    20041 catgatcgct tgaagcgaga tgccgtactt ccgcttgatc gccccaagct cccagagggt
    20101 gagcttgcgg cgccgatgga ccagttccct ctcgagagcc ggccggggaa gaagaagcgc
    20161 cccggcgaaa atatggcaaa gccgctcctt ctccccgtcc ccgtcgaaat cgcagacgat
    20221 gtgggccagc tcatgggccg cggtgaagcg gatccggtca ggcggacggt tcatgttgag
    20281 ggcgatgacg tacccgccgg ccgaccgggc ggccagcccg tcgaaatccc cgaggccgtc
    20341 catgtcccgg atctcaaaga ccttgatgcc gttcgactcg agaagctcga ggaaattggg
    20401 aaccggcgac aggccgagat gccaatgccc gcgcacggtc tcagccgccc tttcaatctc
    20461 ctccgcggtc cgggtggaaa agccggcgac cggattgcgg aaaacgatca cccgtcccag
    20521 gagccgctcc agatcctcgt agcgggccag gtattccagg gcgaggtgct tcaacgcctc
    20581 ctcgcttttt ttcggaagcg gcgcgctggt ccggaattcg aggtaggaca tcgcgtccgg
    20641 ggggagcggc gcgcaaggag aatgaggaac gcgctgacca tgaggcgcgg gtgaagaaac
    20701 gccccttatc tcgattgtcg cttcgttcga gcggccggac caaaggctcg accggcgttc
    20761 cttttcctcg tcgccgcaag ggtaaccatg ggatccgccg tcacgttcgg ccgcgaatcc
    20821 ccccccgtcc ccggaaacct tgagccctcc ggctctttcg gctccggacg cagcccacgg
    20881 cgtatcgcaa taccgcggtt tcaggtcgag gacctgttca agccggttca agatttcggg
    20941 agaaggcgac atcttgccct gctcgtattt ggaaagggcc tggcgggtga cccggccgtc
    21001 gattttccgg gaaagatcct cgagactcaa accggcgatt ttccgggcga ccttcagccg
    21061 cgacggcatg aacagggatt ctttatccat ttcctttcag cttattttcc cgcccgacgg
    21121 ttgacaatat acgccttttc atccgtttgt caaccattat ttttgatgtc ttgtccacga
    21181 tcgaaatgtg aacgtccgga tcatcgtcct ccggcgggag aaccgcatga cccgcacatc
    21241 cgcaacctat tttccggcgg ccgggtaaaa ccgcggcggc cgcccttgaa agccttaaga
    21301 cttagaaagt ctcgccgcgc cgtcctgaaa caaccgctta ttccagcttt cccgtttttt
    21361 cttgtttttg ccgtgccgct gtccggaaag ctcttcgaga agtcgcccgg ccgccgccgt
    21421 catttccagc cgctggagcc cccggtaaaa tcccggaccg gtctccctgt cttcagattc
    21481 cggaccggac gccgcccgcg actccgaaat cgtccgatct ttatcccctt ttgcgggaat
    21541 cgtctcctcg ggcattttat tagatccctc ggcggccgta tccggcgtcg acgggcgatg
    21601 gaccgccgcc ggcgtcctca atcaaaactc tacgggcgtc ccggatatcc gagaaccttg
    21661 agcgcgtccc aggcggccgc gtaaatgccg tccatgatgc acgtgccgag cgacgtcgcc
    21721 ccgtccgatc catcgatcag cggcatcaga acgacaaggg aaacgtccgt ttccgggttg
    21781 tatctcatgg tggaaagata accgtagatg gcgccgttgt ggccatagcc gagattcttt
    21841 acatgatcac aacccaacgc gtagctgccg gcggcgtgcg gaccgatgga attgaccatc
    21901 gtgttgacgg tattcggatg aaggacgttg acgccggaaa acagcgttct gacgaatcga
    21961 ttcaaatcaa tgaaatttcc atatccgttt ccttcggcga catgcgcgct catgttttgc
    22021 gccgaataaa catcggggac cgtgccgccc tccgcgtatt cgatgccgga aacgcacggc
    22081 gccggcaaaa tcgcgtcggt cgcccgatgc ggaaattgaa cgacgctcag gggcacgggt
    22141 gatgagccgc cgatgacatg atcgtataaa tagtcactgt acgttttcgc ggaaccggcg
    22201 cgcgcggagt agacccgggc gatgatctcg ctgagaatgg tatagccggt attgctgtaa
    22261 tgggggacgc cggttccggg ctcgaaataa tagagatccg gatgcgcgac aagttgtccg
    22321 accagctcat cggcgctgaa ctgatgatac ggatcaagag acaactgcca gcgcacgtaa
    22381 gacatcccgt tccagtaatc atcccgggaa tcattatcca catcataaac gccggcggcg
    22441 tgctgcagga gctgctcgat ggtgattcga tctttgtagg gaatgttcca tccggccgtg
    22501 tccggaacgt agggagtcga cgagcccgga atagcggccg tgatatgcga atcgatgtcc
    22561 agccagccgt cctgatgcat tttcagcacc gccgcggccg tgaatgtctt ggtgttgctg
    22621 gcaaagcgga agtgcgttcg ttccgtcagc cgctgcgcgg acaagtccgc ggagctgaca
    22681 aaccatgtgc cgttaagcgc ctgaatgtaa acgctgatgg aagggatcgg cttctccagt
    22741 ttcccggaca accgagttcg gacgctgtcc acggccgact gcatggccat cctttttttt
    22801 tcgtaataaa agcgggagcg ctctctttcc ctcgatttcg gcgcggacgg atccgcggaa
    22861 ccgacgaatg acattccttc aggcttctga agatcagcgc cgatggaggg gatcgcgttc
    22921 cgccgtttcc cggatatctg agttcggacg tttcccgcgg ccgatggcag gaccgttttt
    22981 ctgttttcgt aatgcccgcc aaaactgcag gcggcattca acaacaggga aatcatgata
    23041 atttttgcat aaaaaggctt catgaggccg tttcccccgg atttgatttt cttttaaaat
    23101 gaatcacgcc gaaccccgca catcatattg attttttccc cgtcaatcaa tgaaaaaaat
    23161 aggt
//
LOCUS       DAOOXH010000033        22920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_2070_length_22920_cov_7.439536, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000033 DAOOXH010000000
VERSION     DAOOXH010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 22920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22920
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2070_length_22920_cov_7.439536"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PKV64_05540"
     CDS             complement(<1..517)
                     /locus_tag="PKV64_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPN16578.1"
                     /translation="MYIPLRVHSTFSRGRGGAALDELARAFARDRLPAGALTDIGNLY
                     GWPQWKRAAEGAGFSPLFGCEIEIGGEMFLFLVQNRDGYGNLMEILNRRRIREGDGTE
                     GLAAIWIPGARGGGKPERKDASPDAGGKNGNGENGPPAEPLDIDALLPSLKEGPAAAD
                     LYVGVDFFNAAA"
     gene            complement(523..1935)
                     /gene="dinB"
                     /locus_tag="PKV64_05545"
     CDS             complement(523..1935)
                     /gene="dinB"
                     /locus_tag="PKV64_05545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPN16579.1"
                     /translation="MLNHPPFPPPRTNRRSVAPAFGPADFDGTPAAARAAGSRRPGVL
                     PPVSSPPAPAAVGGPVFRPRLRRFIVHVDIDAFFAAVEVLLNPALKGKPLIVGGLPHE
                     RGVASTCSYEARRYGVHSGMALRTAAKLCPQGVFVRGNSRVYQSFSEKFFEVLGRFTP
                     DVEATSIDEAYLDFTRCRRLYSSFPDAARALKAQVERETGLSVSVGVGPNKILAKLAT
                     GRAKPGGYFEIEAGREEEFLRDVDIDRLPGIGPKTQVVMRMLAIRKIGDLWGLPPATL
                     HSLFGLGGDEIYLQSRGLDNRPLATEAGPKSISRETTFLQDLWDSRLLLAHLAYLCDR
                     LALGLRQGRFYAHIIEVKARYADFRTEVRRRLLVVPRREMTDVYRIARELFLELWGGS
                     RLPLRLVGVKASDLTRVRPLSLFEPYSDRPERLGAAVDRVREKFGFGSVLTIREKMLD
                     EVYPEDRDRGFVLKTASLTR"
     gene            complement(1997..2614)
                     /gene="lexA"
                     /locus_tag="PKV64_05550"
     CDS             complement(1997..2614)
                     /gene="lexA"
                     /locus_tag="PKV64_05550"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPN16580.1"
                     /translation="MVNIIRQELTPRQRDILRAIESFILEHGYGPTIRWLGNFFRIPS
                     PSAVFKHVASLERKGYLRREGGELRLAGSPSALEAHVRVPLAGLVPAGSPREAFEMSR
                     EAVDVPDWMIGRRRGNVFCIRVQGKSMIDAFIDDGDHVLLERTNTVASGEMIVGRLDD
                     GTVTLKRLRRENGRTILVPENPAYQPFEVENLLVLGRVIGILRKY"
     gene            2749..3888
                     /locus_tag="PKV64_05555"
     CDS             2749..3888
                     /locus_tag="PKV64_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HPN16581.1"
                     /translation="MIRRPRFSFLVFALAGLFCLLLQACSPPAGPGHESTRNIIFLIG
                     DGMGNSITSAARARKGGLTGLLRMDEMPVIGLVRTSSTALVTDSAAAGTALAGGVKTN
                     NKSVAMTPDGKKVQSILEAARNRGMATGIVVTSSLADATPACFGAHAPGRWLKSRIAA
                     GLLASGTDLLLGGGSAHFLPRSREGSLRDEERDLVREAGDAGYAVCRTKEELAEAPDG
                     KILGLFARDDMTTEPPEPTLAGMTVEAIRRLGRNRRGFFLLVEGSLIDSRAHDNLLEE
                     TLDQTLKFDEAVGAALDFAKKDGRTLVVVTADHDTGGLSVVDGTVDGAVLKVTWGGDN
                     HTGEAVPLFAFGPGAGLFGGFKDNTEIPRIFARLIGAADFPKISE"
     gene            complement(4115..4531)
                     /locus_tag="PKV64_05560"
     CDS             complement(4115..4531)
                     /locus_tag="PKV64_05560"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPN16582.1"
                     /translation="MDESKLSDDHFCFACGGRNRQGLRIRFEYPEPGRCRAVWTPEKR
                     FQGWKGILHGGIISTLLDEALAHARGAEGRGAGEAAVTAEMTVRFKKPVQIGIPVVLE
                     GRVVSARGRLIECLSTLRDGEGNELASASGKLVKCS"
     gene            4667..5134
                     /locus_tag="PKV64_05565"
     CDS             4667..5134
                     /locus_tag="PKV64_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16583.1"
                     /translation="MNLFCGTSRYVPTAVSQKLVRLIESSAEEMSCSYTREIRSIIEA
                     PSYRTFDEREVHDRGHRVFSQFGRWISNDITEMDMERYWTALGKQRRLEGFAMSEIMI
                     AICLIRREVWLKIRNEGLLDTAEDLYEAMELYGRIVNFFERAHYYTVRGYEGK"
     gene            complement(5140..6246)
                     /locus_tag="PKV64_05570"
     CDS             complement(5140..6246)
                     /locus_tag="PKV64_05570"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPN16584.1"
                     /translation="MAEIKRIGIMTGGGDCPGLNAVIRAVAKRALFEHGWEVFGIEDG
                     YEGMIKARFRRLTAADVSGILTQGGTILGTSNTSDPYAYLLRRGKGTVRRDVSVRAVG
                     HVREKGLDALVLIGGDGTLHTAARLAKAGLRIVGVPKTIDNDLRGTDRTFGFDTAVSV
                     ATEAVDRLHTTAQSHHRIMIVEVMGRRAGWIALYAGVAGGGDIILIPEIPYTLDAVAA
                     KIHFRRSCGKKFSIVVIAEGAKPKGGEAVIHKRIETSVDPVRLGGVGFVLGDELERLT
                     GQEARCVVLGHLQRGGSPTPSDRVLATQLGAEAVRLIARGEFGRMVAVRGGKISSVSL
                     DEPAKGQRLVPLDHPLITAARAVGTSFGDENAGG"
     gene            complement(6257..7729)
                     /locus_tag="PKV64_05575"
     CDS             complement(6257..7729)
                     /locus_tag="PKV64_05575"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPN16585.1"
                     /translation="MTRNKTAVLFLAAALAVSSGCGRTNPLTAKRIKSVEKGLMRAVY
                     LKGLAAEKLSLDERMSFYRVPGVSIAVIDKNELEWAKAYGEKDNQTHQLLTPETLFQG
                     GAFSQMIAGAVVTALAGRGTISLDEDAASGLKSWTFPREFDRRSHALDLRSLLAHGSG
                     LSDQVLLGYGHDEPRPALAEMLAGTKPATNAPLWYTPAGSRLSRTRYAESGYVVLEAF
                     LTDRTGRPFADLAGELVLTPAGMEHSTFEIPLSNGSRELAATGHLRDGRPLAGLWKYY
                     PAAAAKGLWTTPSEFAAFLLDLLRSAGGEGGRILPAASARAMLAPFLENRGPGFMVEG
                     AGDDVQFKLSGKTEGFACQMVLYPVRGQGAVIMTNSDNGFTLIDEILAALSSVYQWPH
                     YRPEEKTVLRLDPETYRGCTGRYEVGPNYFLDVTWEDYYLVIQPTGQAATRFYAEGQT
                     LFYSTDPYIRIQFLRDRGPVEGLVLWQQDFELRAKRIGPK"
     gene            complement(7726..9069)
                     /locus_tag="PKV64_05580"
     CDS             complement(7726..9069)
                     /locus_tag="PKV64_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPN16586.1"
                     /translation="MKDRAIRLGIGFLAAAVLLGAAPGEDLAGKARAMIDALRRGDYE
                     AATRDFDATMRGLSGPEKTGEFWTILQTRIGAFKERTAERRENLGQFEIVLVTCVFEK
                     TTLDARIVFDAEGKIAGFQFVPSSPPVESLPPPYADRERFTETEVTVGSEPWTLPGTL
                     ARPKENGPVPAVVLVHGSGPNDRDETIGPNKPFRDLAWGLASRGIAVLRYEKRTRVYG
                     TKLAADASLTVREEVIDDAVEAVRLLQKTDGLDPRRIFVLGHSLGGMLMPRLALALKP
                     LGAAGFISLAGLTYPLEDTILVQTAYILSLDGGLSGEDRKKLEEVETDVAKIKALTEA
                     DRGSTEKPLNAAPAYWLDLRGYDPLETVVRVDRPMLFLQGGRDYQVVPENLERWKKAL
                     EGRSDAAFKLFPKLNHLFGEGEGISTPSEYLEHPKVVAKDVIDEIEAFVLKGGNS"
     gene            complement(9196..9879)
                     /locus_tag="PKV64_05585"
     CDS             complement(9196..9879)
                     /locus_tag="PKV64_05585"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPN16587.1"
                     /translation="MPNGTSPLGSEAAFDYEKAVRKSENHFVPRFLQINKFLNRPAAS
                     LIVRVFFKTRITPNQVTVASFFIGLAGAGCFGLATPGSFIAGGLLTQLSSIVDCADGM
                     LARARGTSNEFGAHLDLILDRINESFLITGAILGWFRFSGRESDLILGLFALGLYFLQ
                     TTLFYLVKSLTGDERRGETAENRGWLMFLIALFAVINRLDIGLVVLIIASLGGTIGLL
                     IRFFRFRKI"
     gene            complement(9848..10597)
                     /locus_tag="PKV64_05590"
     CDS             complement(9848..10597)
                     /locus_tag="PKV64_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HPN16588.1"
                     /translation="MMQKRDMRGIVLCAGRGGRLSPFTDLTPKCLLSFGGKTILERCL
                     EGLRSAGIGEILLVTGYHRELIERFVRDRGIEGIVFVVNEAYARTNTAVSLNLALKRV
                     SSDVVVVNGDVLFHPSILAELVALPVSHGAAVDADISMDGEEVKVIVRGGRIVRIGKD
                     LNPKDSLGEAIGLYKIGRDSIADLIRIYDGLEAKGEREHFFEKGFDILNGKDAGEDRS
                     FGVFLTRGRPWVEIDTPEDYRHAERDIAPRL"
     gene            complement(10655..11374)
                     /locus_tag="PKV64_05595"
     CDS             complement(10655..11374)
                     /locus_tag="PKV64_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPN16589.1"
                     /translation="MSASRVALVTGASRGLGREIAVALASSAGAVLVHYFRNRRGAEA
                     TARAVRRAGAESDVLQADITVEKEARGLIAAVEKGRGRLDILVNNVGPILFRPWDELT
                     AADWESMFRQNLLGAYFCLSEALPGMRARRFGRIVNLGFGRVEQLAAFPTVLPYAAAK
                     TGLLLLTRTAAAECRGTGVTVNMVSPGLLEGGIRPAGARVPRRELGTYAEVAAAVRFL
                     AAEEARRVTGTDILVAGTWKM"
     gene            11470..12441
                     /locus_tag="PKV64_05600"
     CDS             11470..12441
                     /locus_tag="PKV64_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPN16590.1"
                     /translation="MNEPSRIIVTGASGQIGTELLQALCRKHGPDNVVAADIQPLSAQ
                     APACRFESLDVTKARLLEDILKKHRAGTVYHLAAILSASGEKNPQLAWDVNMSGTFNI
                     LEAARKLGLTRIFIPSSIAAFGPETPRVLTPQETVLRPKTMYGLTKVAGELLADYYVA
                     RFGIDVRGCRFPGIISHGALPGGGTTDYAVAIFYEAVKFGRYSCFLEPDTRLPMMYMP
                     DCLKSIFDLMAADFKNLRHHSDFNVTAMSFTPAELAAEIRRHLPGFEIDYQPDFRQAI
                     ADSWPQSLDDSAARAEWNWKPDYDLAAMTADMIAVLKKRQDEGRLAY"
     gene            complement(12573..13427)
                     /locus_tag="PKV64_05605"
     CDS             complement(12573..13427)
                     /locus_tag="PKV64_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16591.1"
                     /translation="MCRAAVLILAACLAQGSLGIGPLYSGREDVSGRRMSALLEKAAR
                     YCRRLDQAALDFICKEEIAEEIDHAWEWAAEKYFRVQRKENNSYLYDIQFVRKNGIKK
                     ENRALLRRNGRAVLEKDRGVGVPRAIQVEYVLFGPVGLLGEAWQPLRRFRMTGEEESP
                     DGERLVVVEARMRPGTVKGHSDGTAWIRESDGAVLKIEWDGTTAGGYEQIEERGRVFD
                     AAPRLTAVTEYGLEKNGLRFPSRDVTEEAYLKNGKKPFIRSRTESRYTNYRFFTVETE
                     TDLGSSPS"
     gene            13653..14243
                     /locus_tag="PKV64_05610"
     CDS             13653..14243
                     /locus_tag="PKV64_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16592.1"
                     /translation="MSDEQYPLHLRGKRLEELFFFKKDAALADEKRRLQKMVHDLETL
                     SRISGIADEAILRKLIMLDIHVEVLATLSIIPLVEAAWADGKIDDPEREAILKAAEGF
                     GLVEGQVTRDLFEHALRNPPPKGLVESWIFYMQGLCQLLSKEERRALKKDVLRRARTV
                     AEAESGKKKGAARISRRKEEALLRLEQAFEPLRFKI"
     gene            complement(14240..15673)
                     /locus_tag="PKV64_05615"
     CDS             complement(14240..15673)
                     /locus_tag="PKV64_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16593.1"
                     /translation="MERRSSLVPVISLTVVVSFSLSAPGREIEKGRLLDGIACRADAG
                     QSYALYLPSGDDRVELQPVLFLFDPAARGAEGVRAFLSAAEKFGWILIGSNNSKNGPL
                     KDSVAAGRALWAEAKERFPVDENRVYAAGFSGGSRVASIFAQVIGRKIAGVIGCGAGL
                     AEGLGPDGLKAEAYFGLAGLRDFNYEEMKTLDRTLDPSGIPHRILVFDGVHDWPDPVQ
                     CERAAGWMEVLAVKSGRAEKDETMIRSVIDREMDEARSLEKDGRVHWAAERFEAAAGL
                     AEGMIELPGAAGRAEALRKTPAAARFWREEKTRERRTELYRTGVDRALGRIEADETGG
                     AAALAAVLKDLGVRRLKKEAAGAETIENRALASRLLFLLSFSAQVKAASTYERAGLSR
                     TAAFLDLAIAACEEGLSRLKSLYFSRARIATRAGDRKAALDFLGEAVERGFNDIEILD
                     GAEELEGIRNEPRYREIREKLGRLPAA"
     gene            complement(15757..17931)
                     /locus_tag="PKV64_05620"
     CDS             complement(15757..17931)
                     /locus_tag="PKV64_05620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPN16594.1"
                     /translation="MIFLVLFFLAGRPIEFAQEAAFFQGFLIEKPVIRIALDIDLEDA
                     LIHASSGMKIYQAADSYKLLAEDVAEARVKGHKEALTEKFLIQAAQSRRREEAEKIAS
                     ELRLRIDRSVTVAGGKAEKLEGVYQVLVGDFLTRGEALSFIRNLAALGYPEAWIIRQD
                     VPRGASKPQWVMIGGRLVDLNKGSALYLIPSNPQSYLTYNGRNYRGIFILRGSARGAL
                     LVNVLNLEDYLKGVVPGELSPYMFGEIEALKAQAVAARTYALKNLGQYGDLGFDLLAT
                     PQSQVYEGMSVESPRSTRAVEETEGEVAVYRGGLINALYTSTCGGTTENAEEIFEGNP
                     VPYLRSVECVMERERVFSLATARRLPAYFEDGANAAAKIAAVAALGVLEPGRDPEWLR
                     APVSGGEAAEWARKAAAIAGKKDPAATAAPGSLTPPAFARLIRDAFGWEERIRTLVGK
                     SESGHATAGLPGLAPEDRPLMAYFLISGIFPRPTASRAESPVERGEAAAALARVLGLS
                     RDPFHRGILKGIENGFLLVSVDDEIRSVPVAADPYLLRSMEGTVSFVSTLDLEGGDAV
                     EWLERDGRAVYIQAASLPLSNVLDQPSQYHSWQIRISREDLSRRVNQFYPIGKLMDLI
                     PQKRGASRRVVELAVIGQEGRALVRGLKIRQVLNLRDNLFVIDRETDGEGRLTHFLFS
                     GKGWGHGVGLCQVGAFRLAQKGATYKDILKKYYQGIEIEKRS"
     gene            18274..19974
                     /gene="acsA"
                     /locus_tag="PKV64_05625"
     CDS             18274..19974
                     /gene="acsA"
                     /locus_tag="PKV64_05625"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HPN16595.1"
                     /translation="MSNIGNYEERCETFTWEQAEQELEYRPGGVLNIGWHCTDRVCLQ
                     GQGDKTALIWEGMGGNEKRYTFHDIRRASNTIGAFLRGLGLQPEDRICLFMDKIPELY
                     LGVLGILKIGAIAQPLYSAFGDESLFVRLENAQTKAVITQKKHAPKVRKILDKLPHLQ
                     HLIIVDHDGKPLKEREVAFSLEKSAPVETLDIHPTSAESPSLLHYTSGTTGLPKGVKH
                     VHYSLLSQYMTAKWALDIRDDDIFWCTADPGWVTGTSYGVIGPWCLGVTQCVLDSGFS
                     AEAWYRFIEKNRITNWYSAPTAIRSLMKAGEEVVRKYDLSSLRALSSVGEPLNSEAVL
                     WSERVFGKPFLDTYFQTETGSIMITNFQGMKIKPGSMGKPFPGITAVILDQKTFEPYA
                     EPGKIGLIALKPGWPAMMRMYWNNEETYRKKFNNGWYLTGDRASIDKEGYFWFVGRDD
                     DIINTGGHLVSPFEVESALIEHPAVAESAVVSKPDTINMEVVKAFVTLKAGFTPSADL
                     DLEIMNFIRKKLSPLAMPQEIEFTEALPKTRSGKIMRRILKAKEWGEEIGDTSTLEND
                     "
     gene            20037..20288
                     /locus_tag="PKV64_05630"
     CDS             20037..20288
                     /locus_tag="PKV64_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPN16596.1"
                     /translation="MSDELKDIVLKYVIDEYLEDDSEPITYDTPLISGGIVDSFSMVS
                     LKRFLENKYKISIPDDQATPEAFDSVDKIAVLVKKFTKA"
     gene            20377..21624
                     /locus_tag="PKV64_05635"
     CDS             20377..21624
                     /locus_tag="PKV64_05635"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPN16597.1"
                     /translation="MSYSEKTRAAYQEVLQGLKDAGLFKQERFIHSSQAADIEVEFPA
                     GASVRKVINMCANNYLGLSSHPAVVQAAHEGMDARGYGMSSVRFICGTQDIHRDLERK
                     VTEFLGTEDTILFPSCMDANAGVFEAVLAKDDVMISDRLVHASLIDGIRLCGAMQDTY
                     KHADMAHLEQKLEMHQDRRFRMVITDGVFSMDGDTAPLDKMAALCDKYDAMILVDDSH
                     ATGFIGRTGRGTHEKYGVVGRMDIITTTFGKALGGATGGCVSGRRELVEMCRQKARPY
                     LFSNSIAPVVVQGILKVFDILMASTERRDKLEKNAAYWRKGLQEAGFVLKEGDTPIVP
                     VMLFNAKLAQDFSRALYEEGIYAVGFFFPVVPQGQARIRTQISAGHEIHHLDKALEAF
                     RKVGKTFDILGRTKQEIIERFGM"
     gene            21640..22212
                     /locus_tag="PKV64_05640"
     CDS             21640..22212
                     /locus_tag="PKV64_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HPN16598.1"
                     /translation="MDNKLANPAPLGLMGFGLTTVLLNIHNAGFFPLSAMVLAMGIFY
                     GGLAQVIAGIMEFKKGNSFGTTAFTSYGLFWLTLVATWVIPGANAPNGGATPAPYLGW
                     YLFLWGLFTFLMWIGTFGKNRALQFVFLSLWILFLLLAVRDWTGSETIGKIAGFEGIV
                     CGLSAVYLAMAEIINEARGRTILPIGASKA"
     gene            22516..>22920
                     /locus_tag="PKV64_05645"
     CDS             22516..>22920
                     /locus_tag="PKV64_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16599.1"
                     /translation="MKGRRLDRLLTAVAAAAAATVLVLVLAAGGQTPAGPGSDTSGVG
                     AYIPRQLVVLAKTPQADKDVKALANALGAVIQAEIPQGRLFLFVFSDDATAGNALLKI
                     KKDNKESFDAWRNWKFSIPKPARPPKKTHAGAA"
BASE COUNT         4312 a         7565 c         6923 g         4120 t
ORIGIN      
        1 ccgcggccgc gttgaagaaa tccaccccga catagaggtc ggccgccgcc gggccctcct
       61 tgagcgaggg cagcagggcg tcgatatcca gcggctccgc cggcgggccg ttttctccgt
      121 ttccgttttt tcccccggcg tccggggagg cgtccttccg ctccggcttt cctcctcccc
      181 tcgctcccgg aatccaaatc gccgccaggc cctcggtccc gtcgccctcc cggatccggc
      241 ggcggttgag gatttccatg aggtttccat agccgtcccg gttttggacc aggaagagaa
      301 acatctcgcc gccgatttcg atctcgcagc cgaaaagggg agaaaacccg gccccctccg
      361 ccgcccgctt ccattggggc cagccgtaga gatttccgat gtcggtcaag gctccggctg
      421 ggagacggtc ccgggcaaag gcccgggcca gctcgtcgag agccgcccct ccccgccccc
      481 ggctgaacgt ggaatggacc cgcagtggaa tatacacggc cgtcaccggg tcagggaagc
      541 cgttttgagg acgaacccgc ggtcccggtc ctccgggtag acttcgtcca gcatcttttc
      601 ccggatggtc aggacggagc cgaatccgaa tttctcccgg acccggtcca cggccgcgcc
      661 cagccgttcc ggccggtcgg aatacggctc gaagagagac agcggccgga cccgggtcaa
      721 atccgaggcc ttgaccccca caagccggag aggcaggcgc gacccgcccc acagctcgag
      781 gaaaagctcc cgggcgatcc ggtagacgtc cgtcatttcc cggcgcggga cgacgagcag
      841 gcggcgacgg acctcggtcc ggaagtcggc gtagcgggcc ttgacctcga tgatgtgggc
      901 gtagaaccgg ccttggcgca ggcccagggc cagccggtcg caaagataag ccaggtgggc
      961 caggagaagg cggctgtccc agagatcctg gagaaaggtc gtctcccggg aaatggactt
     1021 ggggccggcc tcggtcgcca ggggccgatt gtcgaggccg cgggattgaa ggtagatctc
     1081 gtccccgccc aacccgaaga gggaatgaag cgtcgccggg ggcaggcccc agaggtcgcc
     1141 gattttccgg atggcgagca tccgcatgac gacctgggtc ttgggcccga tgccggggag
     1201 ccggtcgatg tcgacgtcgc ggagaaactc ctcctcccgg ccggcctcga tctcgaaata
     1261 tccgccgggc ttggcccgcc ccgtggccag cttggccagg atcttgttcg gcccgacccc
     1321 gacggaaacc gaaaggccgg tctcgcgctc gacttgggcc ttgagggccc gggcggcgtc
     1381 cggaaaggag gaatagagcc ggcggcagcg ggtgaaatcg agatacgcct cgtcgatcga
     1441 cgtcgcctcg acgtcgggcg tgaagcggcc gaggacctcg aaaaactttt ccgagaagga
     1501 ctggtagacc cgggaattgc cgcggacgaa gacgccctgg ggacaaagct tggcggcggt
     1561 ccgcagggcc atgccggagt ggacgccgta gcggcgggct tcgtaggagc aggtcgaggc
     1621 cacgccgcgc tcgtggggca ggccgccgac gatcaacggc ttgcctttca gggccggatt
     1681 caggaggacc tccacggcgg cgaaaaaagc gtcgatgtcg acatggacga tgaagcggcg
     1741 gaggcggggg cggaacaccg gcccgccgac ggcggccggc gccggcgggg aggaaacggg
     1801 cgggaggacg ccggggcgcc gcgatcccgc ggcccgggcg gcggcgggcg tcccgtcgaa
     1861 gtccgccggc ccgaaggcgg gggcgacgga ccggcgattc gtccgcggcg gcggaaacgg
     1921 cggatgattc aacatggttc ctcaaaaaac gacgggcggg agtgaacccg cctctatttc
     1981 aaaacaacga tcccggtcaa tacttcctca aaatcccgat cacccgtccc aggaccagga
     2041 ggttttcgac ctcgaagggc tggtaggcgg ggttctcggg gacgagaatg gtccggccgt
     2101 tttcccgccg gagccgcttg agggtgaccg tcccgtcgtc cagccggccg acgatcatct
     2161 cccccgaggc gacggtgttg gtccgctcga gaagaacgtg gtcgccgtcg tcgatgaagg
     2221 catcgatcat gctcttgccc tggacccgga tgcaaaacac gttcccccgc cggcggccga
     2281 tcatccaatc gggaacgtcc acggcctccc gcgacatttc gaacgcttcc cggggggagc
     2341 cggcgggaac gagaccggcc agggggaccc ggacatgcgc ctccaaggcg gacggcgagc
     2401 ccgccagccg cagctctcca ccctcgcgcc gcaggtaacc cttgcgctcc aggctggcca
     2461 cgtgcttgaa gacggccgaa gggctgggaa tccggaagaa attcccgagc cagcggatcg
     2521 tcggcccgta gccgtgctcc aagatgaaag actcgatggc ccggagaatg tcccgctggc
     2581 gcggggtgag ctcctgtctt attatgttca ccattttgtt caccatggct aagatacccc
     2641 ccgccgaagc gtttgtcaag ggccaaaatc atccttgacg gaaattccgc ccccgtgata
     2701 tcgtttccct tcaaattccg gacggatccg atttttcgag gattccaaat gattcgacga
     2761 ccgcggtttt cctttcttgt cttcgccctc gccggccttt tctgccttct ccttcaggcc
     2821 tgctctcccc cggccggccc cgggcatgag tcgacccgga acatcatttt tctcatcggc
     2881 gacgggatgg gcaactcgat cacctcggcc gcccgggcca gaaaaggcgg cctgaccggt
     2941 cttcttcgaa tggacgagat gcccgtcatc ggcctcgtcc ggacatcctc gaccgccctt
     3001 gtcaccgatt cggccgcggc cgggacggcc ctcgccggcg gcgtgaagac caacaacaaa
     3061 tccgtggcga tgactccgga cggaaagaag gtccagtcca tcttggaggc cgcccggaac
     3121 cgcgggatgg ccaccggaat cgtcgtcacg tcctctcttg ccgacgccac gcccgcctgc
     3181 ttcggagccc acgccccggg ccgctggctg aaaagccgga tcgccgcggg tctcctggcg
     3241 agcgggaccg atcttctcct cggcggcggt tccgcgcatt tcctcccccg ctcccgcgaa
     3301 ggaagcttgc gcgacgaaga gcgcgacctt gtccgcgaag ccggggacgc cggctatgcg
     3361 gtctgccgaa ccaaggagga gctggccgaa gctcccgatg gaaaaatcct cggcctgttc
     3421 gcccgggatg atatgaccac cgagccgccc gaacccacgc tggccgggat gaccgtcgag
     3481 gccatccgcc gcctgggccg gaaccggcgc ggctttttcc tcctggtcga gggcagcctg
     3541 atcgattcgc gggcccacga taatctcctg gaagagaccc tcgatcaaac cttgaaattc
     3601 gacgaggccg tcggtgccgc cctcgatttc gcgaaaaagg acggccggac gctcgtcgtc
     3661 gtcaccgccg accatgacac gggaggattg agcgtcgtcg acgggacggt cgacggcgcg
     3721 gtccttaaag tgacttgggg aggggacaat cacaccgggg aggccgttcc cctcttcgcc
     3781 ttcggcccgg gcgccggctt gttcggcgga ttcaaggaca acaccgagat cccccggatt
     3841 ttcgcccggc tgatcggcgc ggccgacttt ccgaagatct cggaatagct ccggtcgaaa
     3901 cgcccggccg gggcgctcac ggatgcgtct tgatcatcgg ctccatgacc tcgaaaactc
     3961 cgttgattcc cggatgaggc ccctcgcgca tcgattgcga tcggatcgtg attcggacgt
     4021 ctgaaaaacg cccgtcgctc ctttcgacac cgcctccgcg ccgaggtcaa atgcctttgg
     4081 tcaaaagggc gatcggcccc gccccgaagc ccggtcaaga acatttcacg agcttgccgg
     4141 aggcggaggc gagctcgttt ccctcgccgt cgcggagggt ggaaaggcat tcgatcaggc
     4201 ggccccgggc cgaaacgacc cggccctcga gaacgaccgg aatcccgatt tgaaccggct
     4261 tcttaaacct caccgtcatt tcagccgtga cggcggcttc acccgccccc cggccttccg
     4321 cgccgcgggc gtgggccagg gcttcgtcga gcagggtcga gatgatcccc ccatggagga
     4381 tgcccttcca gccttggaag cgtttttcgg gcgtccaaac ggcccggcac cggcccggtt
     4441 cggggtactc gaaccggatc cgcaaccctt gccggttccg gccgccgcag gcgaaacaaa
     4501 aatgatcatc gctcagcttt gattcgtcca tccgcctctc ctccctccga tccattatcc
     4561 tttcccgccc ggtcgtgtca aggaaagacg gaaggaaaag aaaggatttt ttggggttga
     4621 caagccgcct ccccggaacc tatctttccg ttagggagag ttgtctatga atcttttctg
     4681 cggaaccagc cgctatgtgc ccaccgccgt ttcccagaag ctcgtccgcc tcatcgagtc
     4741 gagcgccgag gagatgtcct gctcctatac ccgggaaatc cggtcgatca tcgaagcccc
     4801 gtcctaccgg accttcgacg agcgggaggt ccacgaccgg ggccaccgcg ttttcagcca
     4861 attcggccgc tggatttcca acgacataac ggaaatggac atggaacgct actggacggc
     4921 cttaggcaag cagcgccgcc tcgagggctt cgccatgtcc gagatcatga tcgccatctg
     4981 cctgatccgg cgcgaggtct ggctgaaaat ccgcaacgag ggcttgctcg acacggccga
     5041 ggatctctac gaggccatgg agctctacgg acggatcgtc aatttctttg aacgagccca
     5101 ttactacacc gtccggggat acgagggaaa ataaaagcct tatccgccgg cgttctcgtc
     5161 gccgaagctc gtcccgacgg cccgggcggc ggtgatcagc gggtgatcaa gcggaaccag
     5221 gcgctgtccc ttcgccggct cgtccaggga aacggatgaa atcttcccgc cccggacggc
     5281 gaccatccgg ccgaactccc cccgggcgat caagcggacc gcttccgctc cgagctgggt
     5341 cgccaggacc cggtcggagg gagtgggcga gccgccgcgc tggaggtgtc ccaggacgac
     5401 gcaccgggct tcctggcccg tcagccgctc gagctcgtcg ccgaggacga aacccacgcc
     5461 gcccaggcgg accggatcga cgctcgtctc gatccgcttg tggatcacgg cctctccacc
     5521 tttcggcttg gctccctcgg caatgacgac gatgctgaat ttcttgccgc acgatctccg
     5581 gaagtggatc ttggcggcga cggcgtccaa ggtgtacggg atctccggaa tcaggatgat
     5641 gtcccctccg ccggcgactc cggcataaag ggcgatccag ccggcccgcc ggcccatgac
     5701 ctcgacgatc atgatccgat gatgggactg agcggtcgtg tggagccggt cgacggcctc
     5761 ggtggccacg ctcacggccg tgtcgaagcc gaaggtccgg tccgttcccc gcaagtcgtt
     5821 gtcgatcgtc ttcgggacgc cgacgatccg aagccccgcc ttcgcgaggc gggcggccgt
     5881 gtgcagcgtt ccgtcccctc cgatgaggac cagggcgtcg agccccttct cccggacatg
     5941 gccgacggcc cggaccgaca cgtcgcggcg gactgttcct ttccccctcc ggaggagata
     6001 ggcgtacgga tcggacgtgt tcgaggtccc caggatcgtt ccgccctggg tcaggatccc
     6061 ggaaacgtcg gcggccgtca gcctccgaaa ccgcgcttta atcatcccct cgtagccgtc
     6121 ctcgatcccg aacacctccc agccgtgctc gaacagggcc cgcttggcca ccgcccggat
     6181 gaccgcgttc aatccggggc aatcccctcc cccggtcatg attccgatcc gcttgatttc
     6241 ggccatggga caacctctat ttcggaccga tccgcttggc ccgcagctcg aaatcctgct
     6301 gccagagcac gaggccctcg accggccccc ggtcccggag gaactggatc cgaatgtagg
     6361 ggtcggtgga atagaacagg gtctggccct cggcgtaaaa ccgggtcgcg gcctggccgg
     6421 tcggctggat gaccagataa taatcctccc aggtcacgtc caggaaatag ttcgggccga
     6481 cttcgtacct cccggtgcag ccccggtagg tttcgggatc gaggcgcagg accgtttttt
     6541 cctccggccg gtaatggggc cactggtaga cggaggagag agcggccagg atttcatcga
     6601 tcaaggtgaa gccgttgtcg ctgttggtca tgatcaccgc gccctgtccc cggacggggt
     6661 acagaaccat ctggcaggcg aatccctccg tctttccgct caacttgaat tgcacgtcgt
     6721 cgcccgcgcc ctcgaccata aagcccgggc cccggttttc caggaagggg gcgagcatcg
     6781 cccgggccga ggcggccggg agaatccgcc ccccctcccc gccggccgac cggagaagat
     6841 ccaggagaaa ggcggcgaat tccgacggcg tcgtccagag gcccttggcg gccgcggccg
     6901 ggtaatattt ccaaagcccg gcgagcggcc ggccgtcgcg caggtgcccg gtggcggcca
     6961 gctcccgcga cccgttggaa agcgggattt cgaacgtgct gtgttccatc ccggccggcg
     7021 tcaggacgag ctcgccggcg aggtcggcga acggccggcc ggtccggtcg gttaaaaaag
     7081 cttctaaaac gacgtaaccc gactcggcgt agcgcgtccg ggacaggcgg ctcccggccg
     7141 gcgtgtacca cagcggcgcg ttggtcgccg gcttcgttcc ggccagcatt tcagcgagcg
     7201 cgggccgcgg ctcgtcgtgc ccgtatccga gaaggacctg gtcggaaagc ccgctcccgt
     7261 gggctaaaag cgagcggagg tcgagggcgt ggctccgccg gtcgaactcg cgcggaaacg
     7321 tccaggattt caagcccgag gcggcgtcct cgtccaggga gatcgtcccc cgcccggcca
     7381 gggccgtgac gaccgcgccg gcgatcatct ggctgaacgc cccgccttgg aaaagagttt
     7441 ccggggtcag gagctgatga gtctgattgt ccttttcgcc gtaggccttg gcccattcca
     7501 gctcgttttt atcgatcacg gcgatcgaaa cgccggggac gcggtaaaaa gacatccgct
     7561 cgtccaagga cagcttttcg gcggccagtc ctttcagata gaccgcccgc atcagccctt
     7621 tttcgaccga cttgatcctc ttggccgtca agggattcgt ccggccgcag ccggaggaaa
     7681 ccgccaaggc cgcggcgagg aaaagaaccg ccgttttgtt ccgcgtcatg aattccctcc
     7741 cttgaggacg aaggcctcga tttcatcgat cacgtccttc gccacgactt tgggatgttc
     7801 gagatattcg ctcggcgtcg aaatcccctc cccttcgccg aacaaatggt tgagcttcgg
     7861 gaacagcttg aacgccgcgt ccgaacggcc ttccaaggcc ttcttccacc gttccaggtt
     7921 ttctggaaca acctggtaat cacgcccgcc ttggagaaag agcatcggcc ggtccacccg
     7981 gacgacggtc tccagcggat catacccgcg gagatcaagc cagtaggccg gggcggcgtt
     8041 taacggcttt tccgtcgagc cgcggtccgc ctcggtcaag gccttgatct tcgcgacgtc
     8101 cgtctcgact tcctccagct tcttgcggtc ctctccggac aagccgccgt ccaacgacag
     8161 gatgtaggcc gtctggacga gaatcgtatc ctccagcgga taagtcagcc cggccaggga
     8221 aatgaaaccg gccgccccga gcggcttgag cgccagggcg aggcggggca tcagcattcc
     8281 cccgagactg tggccgagga cgaaaatccg ccgggggtca aggccgtccg tcttctgaag
     8341 aaggcggacc gcctcgacgg cgtcgtcgat gacctcctcc cggaccgtca aggacgcgtc
     8401 agcggccagc ttggttccgt agaccctcgt cctcttttcg tagcgcagga ccgcgatccc
     8461 gcgcgaggcc agcccccagg ccaggtcacg gaagggctta ttgggcccga tcgtctcgtc
     8521 gcggtcgttc gggccggaac cgtggaccaa gaccacggcc gggaccggtc cgttttcctt
     8581 cggccgggcc agggtgccgg gaagcgtcca aggctcggag cccaccgtga cctcggtttc
     8641 ggtgaatcgc tcccggtcgg cgtaaggcgg aggaaggctc tccaccggcg gacttgacgg
     8701 gacgaattgg aagccggcga ttttcccttc cgcgtcgaaa acaatccggg cgtcgagggt
     8761 cgtcttttcg aagacgcagg tcaccaggac gatctcgaat tgcccgagat tctcccggcg
     8821 ttcggccgtc cgttccttaa aggccccgat ccgggtctga aggatcgtcc agaattcccc
     8881 ggttttttcc ggtcccgaca gaccgcgcat cgtcgcgtcg aaatcccggg tggccgcctc
     8941 gtaatctccg cgccgcaggg cgtcgatcat cgcccgggct ttcccggcaa ggtcttcgcc
     9001 gggtgccgcc cccaagagga cggcggccgc taaaaagccg attccgagtc gaatcgcgcg
     9061 gtctttcatt ttcgttcgtt ccttttcccg cgccggcgtc gtctccggcg gccgggatga
     9121 tcgatcgggt ctttttagga taaccgaatc ccccggacaa ataaataccg gggaacggga
     9181 aaggacggct tcccgtcaga tttttctgaa ccggaaaaac cggatcagca gaccgatggt
     9241 gccgccgagg gaggcgatga tcaggacgac gagaccgata tcgaggcggt tgatcacggc
     9301 gaacagggcg atgagaaaca tcagccagcc gcggttttcc gcggtctccc cccgccgctc
     9361 gtccccggtc aggcttttca ccaggtaaaa cagggttgtt tggagaaaat aaagccccaa
     9421 ggcgaataaa ccaaggatca ggtccgactc ccggccggaa aatcggaacc agcctaaaat
     9481 cgccccggtg atcaggaagg attcgttgat ccggtcgagg atgaggtcga gatgagcgcc
     9541 gaattcattg gaagtccccc gggctcgggc cagcatgccg tcggcgcaat cgacgatgga
     9601 ggagagctgg gtcaggagac cgccggcgat aaacgagccg ggcgtggcca agccgaagca
     9661 cccggccccg gccaagccga tgaagaagga ggcgaccgtc acttggttgg gagtgatcct
     9721 cgtcttgaag aaaacccgga cgatcaacga ggcggccggc cggttgagaa acttgttgat
     9781 ttggagaaag cgggggacga aatggttctc gctcttgcgg accgctttct cgtagtcgaa
     9841 cgccgcctca gagccgaggg gcgatgtccc gttcggcatg acgataatcc tcgggcgtgt
     9901 cgatttccac ccaggggcgg ccccgggtca ggaagacgcc gaatgaacgg tcctcgccgg
     9961 cgtctttccc gttcaggata tcaaaccctt tttcaaaaaa atgctccctc tcgcccttcg
    10021 cctcgagccc atcatagatc cggatcaagt cggcgatcga gtcccggccg attttataaa
    10081 gtccgatggc ctcgccgaga ctgtccttcg gattcagatc cttgccgatc cggacgattc
    10141 gtccgccgcg gacgatgacc ttcacttcct ctccatccat ggagatatcg gcgtcgacgg
    10201 ccgcgccgtg ggagacgggc agggcgacga gctcggccag gatcgagggg tggaagagaa
    10261 cgtcgccgtt gacgacgacc acgtccgagg acacgcgctt caaggccagg ttcaaggaaa
    10321 cggccgtgtt ggtgcgggca taggcctcat tcacgacgaa gacgatcccc tcgatccccc
    10381 ggtcccgaac aaagcgctcg atgagctccc ggtggtagcc ggtgacgagg aggatttccc
    10441 cgatcccggc cgaccgcaag ccttccaggc accgttcgag gatcgttttc ccgccgaaag
    10501 acaaaaggca tttcggcgtt aggtcggtga agggactgag gcgtccgccg cgtccggcgc
    10561 aaaggacgat tccccgcatg tcgcgtttct gcatcatcgg tcagccgata ttatgtccat
    10621 tttcgggatt ttttcaatcg gaaaatccgt ccgctcacat tttccacgtc ccggcgacca
    10681 ggatgtcggt cccggtgacg cgccgggctt cctcggccgc gagaaaccgg acggccgcgg
    10741 ccacttcggc gtatgttccc agctcccgcc gcgggacgcg cgccccggcc gggcggatcc
    10801 ccccctcgag cagccccggc gataccatgt tgaccgtcac ccccgtcccc cggcattcgg
    10861 ccgcggccgt ccgggtcaag aggagaaggc ccgtcttggc cgcggcgtag ggaagcaccg
    10921 tcggaaaagc ggccagctgt tccacccgcc cgaaacccaa gttgacgatc cgtccgaacc
    10981 gccgggcccg catccccggc agggcctcgc tcaggcaaaa atacgccccg agaaggtttt
    11041 gccggaacat ggattcccaa tcggcggccg taagctcatc ccacggccgg aagagaatcg
    11101 ggccgacgtt gttgaccagg atgtccagcc ggccccttcc cttctcgacg gccgcgatca
    11161 gcccgcgggc ctccttctcc accgtgatgt cggcttgaag gacatcgctt tccgctccgg
    11221 cccggcggac cgcccgggcc gtcgcctcgg cgcctctccg gttccggaaa taatggacga
    11281 ggaccgcccc cgcggaagag gccagggcga cggcgatttc ccggccgagg ccgcgggaag
    11341 cgccggtgac aagggcgacg cgggaagcgg acatggccct agtatcctct ttttcccggt
    11401 ttgatttcaa cggacgtcta tgatatttaa tccccccttg gaatatccgc tgaaacccga
    11461 ggcatttgca tgaatgaacc ctcccgcatt atcgtcaccg gcgcgtccgg acagatcgga
    11521 accgagcttc tccaagccct gtgccggaaa cacggcccgg acaacgtcgt cgccgccgac
    11581 atccagcctc tttcggccca ggcgcccgcc tgccgcttcg aatccctcga cgtgacgaag
    11641 gcccgacttc tcgaggacat cctcaagaag caccgggccg ggacggtcta ccatctggcc
    11701 gcgatcctct cggcctccgg agaaaagaat ccccagctgg cctgggacgt caacatgagc
    11761 gggacgttca acatcctgga agccgcccgg aagctcggct taacccgaat tttcatcccc
    11821 agctcgatcg ctgctttcgg tcccgagacg ccccgggtcc tcacccccca ggaaacggtc
    11881 cttcggccca agacgatgta cggcctgacg aaggtcgccg gcgagctttt agccgattat
    11941 tatgtcgccc gcttcggaat cgatgtccgc ggctgccggt tccccgggat catcagccat
    12001 ggagcccttc ccggcggcgg aacgaccgat tacgccgtgg cgatttttta tgaagcggtt
    12061 aaattcggcc ggtattcttg ttttctcgaa ccggacacgc ggctcccgat gatgtacatg
    12121 cccgattgct tgaaatcgat tttcgatctg atggccgccg acttcaagaa cctccgccac
    12181 cactccgatt tcaacgtcac ggccatgagc ttcactccgg ccgagctggc cgccgagatc
    12241 cgccgccatc tccccggttt tgaaatcgat tatcagccgg acttccgcca ggccatcgcc
    12301 gactcctggc cccaatccct ggacgactcg gccgcccggg cggaatggaa ctggaagccg
    12361 gattatgact tggccgcgat gaccgccgac atgatcgccg tcctgaagaa gcggcaggac
    12421 gagggccgtc tcgcctactg aaaagagaaa tccgggcggg gcgaaacctc tttctctctt
    12481 ccccgccttt ccggcttacg gaaaccacgg gaagaaggct gtcccgagaa gacacggatc
    12541 cccatcctct tgtttttcat caccgctgga aatcaagaag gcgacgaccc gagatccgtc
    12601 tcggtctcga cggtgaaaaa ccggtaattc gtgtaccggc tctccgtgcg cgagcggatg
    12661 aacggctttt tcccgttttt gaggtaagcc tcttccgtga cgtcgcggct cgggaagcga
    12721 agcccgtttt tttccaagcc gtattccgtg accgccgtga gccggggcgc ggcgtcgaaa
    12781 acccgtcccc tctcctcgat ttgctcgtag ccgccggcgg tcgttccgtc ccattcaatc
    12841 ttgaggacgg ctccatcgct ttcccggatc caggccgttc cgtcggagtg gcctttcacc
    12901 gtccccggac gcatccgcgc ttcgacgacg accagccgct ctccgtccgg cgattcttcc
    12961 tcgcccgtca tccggaagcg gcggaggggc tgccacgctt cccccaaaag accaaccggg
    13021 ccgaacagga cgtattccac ctggatcgcc cgagggacgc cgacgccccg gtccttttcc
    13081 aagaccgccc gtccgttcct tctcagaagc gcgcggtttt ccttctttat gccgtttttc
    13141 cggacgaact gaatatcata gagataggag ttattttctt tccgctgaac ccggaaatat
    13201 ttctccgccg cccattccca ggcatgatcg atttcctcgg cgatctcttc tttgcagatg
    13261 aaatccaaag ccgcctggtc caaccggcgg cagtagcggg ccgccttttc cagaagcgcc
    13321 gacatccggc gtccggagac gtcctcccgt ccggaataaa gcggcccgat ccccaaggaa
    13381 ccctgggcca ggcaggcggc gagaataagg acggcggcac gacacatggt ttcctcttta
    13441 tttaaacgca gccgcgggcc gcggggatgc cgtccgaact ccagcgagag cggccggata
    13501 aacccggatg cctgatcgga agccgccgcg tcctcgccgc tttcggcccg cccatcgggg
    13561 aagagaacca tgcggaaaaa tatcataatc cgtcccccgc ttcatcgtca accgcttgac
    13621 tccgcccttc gaccggacta tatttcgggc gcatgagtga cgagcaatat cctctccatc
    13681 ttcggggcaa gcgtctcgag gagctgttct ttttcaagaa ggacgccgcc ctggccgatg
    13741 aaaaacggcg actccaaaaa atggtgcatg atctcgaaac cctctcccgg atctccggca
    13801 tcgccgatga ggccatcctc cgaaagctga tcatgctcga tatccacgtc gaggtcctcg
    13861 ccaccctctc gatcatcccg cttgtcgagg cggcctgggc cgatggaaag atcgacgatc
    13921 ccgagcggga agcgatcctt aaggccgccg aaggcttcgg cctcgtcgag gggcaagtca
    13981 cccgggattt gttcgaacac gctctccgga atcctccgcc caagggcctg gtcgaatcct
    14041 ggatcttcta catgcagggc ctttgccagc ttctcagcaa ggaagagcgc cgggcgctca
    14101 agaaagacgt tctccggcgg gcccggacgg tcgccgaggc ggaaagcggg aaaaagaaag
    14161 gcgccgcccg gatttcgcgg cgtaaagaag aagcgctgct ccggctggaa caggctttcg
    14221 aaccgctccg cttcaagatt taggccgccg gcaagcggcc cagtttctcc cggatctccc
    14281 gataacgggg ctcattcctg attccctcca gctcttcggc gccgtccaaa atctcgatgt
    14341 cgttgaatcc cctctcgacc gcctccccga gaaaatcgag ggctgctttc ctatctccgg
    14401 cccgggtcgc gattctcgcc cggctgaagt aaagcgattt cagccgggac aagccctcct
    14461 cgcaggcggc gatggccagg tcaaggaaag cggccgttcg cgaaagcccg gcccgctcgt
    14521 atgtcgaggc cgccttgact tgggcggaga aactgagaag gaagagaagg cgggaggcca
    14581 gggcgcggtt ttcgatcgtt tcggctccgg ccgcttcctt cttcagacgc cgaaccccta
    14641 aatccttgag aacggcggcg agggcggccg cgccgcccgt ttcgtcggcc tcgatccggc
    14701 cgagcgcccg gtccacgccc gttcgataaa gttccgtccg tcgttccctc gttttctcct
    14761 ctctccaaaa gcgcgccgca gccggcgttt ttctcaaggc ctccgcccgg ccggccgctc
    14821 cgggaagctc gatcattcct tccgccaggc cggcggcggc ctcgaatcgt tcagccgccc
    14881 aatgaacgcg gccgtctttt tccaacgagc gggcttcgtc catctcccga tcgatgaccg
    14941 accggatcat cgtctcgtcc ttctcggcgc gcccgctctt gacggccagg acttccatcc
    15001 agccggcggc ccgttcgcac tggaccgggt ccggccagtc gtgaacgccg tcgaaaacga
    15061 ggatccggtg cggaatcccg gacggatcga gggttcggtc gagagttttc atctcctcgt
    15121 aattgaaatc gcgcagcccc gccagcccga aatacgcctc ggccttcaag ccgtccggac
    15181 cgaggccttc ggccaggccg gctccgcagc cgatgacgcc ggcgatcttc cggccgatga
    15241 cctgggcgaa gatcgaagcc actcgggaac cgcccgagaa gccggccgca tagacccggt
    15301 tctcatccac ggggaaccgc tccttggctt ccgcccaaag agcccggccg gccgcgacgc
    15361 tgtctttcag cggtccgttt ttcgaattat tcgagccgat aaggatccag ccgaattttt
    15421 cggccgcgga cagaaacgcc cggacgcctt ccgcccctcg agcggccggg tcgaagagaa
    15481 agaggacggg ctggagctcg acccgatcat ctccggatgg aagatagagg gcgtagctct
    15541 gcccggcatc ggcccggcag gcgatcccgt caagaagcct gcccttttcg atttcccgtc
    15601 ccggggcgga caaagaaaag gagacaacga cggtcaacga aatgaccggg acgagggacg
    15661 atcgtctctc catgggcctt tctccgcctc gttcaggcta acgcctgaag gagaagacgt
    15721 caagatggat cgcgaacgga atgccggccg gggcggtcag gatcgttttt cgatctcgat
    15781 gccctggtaa tactttttca gaatgtcctt gtacgtcgcg cccttctggg ccaggcggaa
    15841 ggctccgacc tggcagaggc cgacgccatg cccccagccc ttcccggaaa agaggaaatg
    15901 ggtgaggcgg ccctcgccgt cggtctcccg gtcgatgacg aagagattat cccggagatt
    15961 gaggacctgg cggattttaa gcccccgaac aagcgcccgg ccttcctggc cgatcacggc
    16021 cagctcgacc acccggcggg aggcgcccct tttctggggg atcaggtcca tcagcttgcc
    16081 gatcgggtaa aactggttca cccggcggct caggtcctcc cgggatatcc ggatctgcca
    16141 gctgtgatat tgggaaggct ggtcgaggac gttggacaac ggaagagagg ccgcctggat
    16201 ataaaccgct cggccgtcgc gctccagcca ttccacggcg tcgcccccct ccaaatcaag
    16261 cgtcgagacg aaggaaaccg ttccctccat gctcctgagg agatacggat ccgccgccac
    16321 ggggacggac cgaatctcgt cgtccacgga aacgagcagg aagccgtttt cgatcccttt
    16381 caggattccc cggtggaagg gatcgcggga caatcccagg acccgggcga gggccgcggc
    16441 cgcctccccc cgttcgaccg gagattcggc ccgggaggcg gtcggccgag gaaagattcc
    16501 ggagattaaa aaataggcca tgagcggacg gtcttcggga gccaggccgg gaaggccggc
    16561 cgtcgcgtgc ccggactcgc tttttccgac gagcgtccgg atccgttcct cccagccgaa
    16621 cgcgtcccgg atcagacggg cgaaggccgg cggcgtcagg gatcccgggg cggcggtcgc
    16681 ggccggatct ttttttccgg cgatcgccgc ggctttccgc gcccattccg ccgcttcccc
    16741 gccggagacc ggagcccgga gccattcggg atcccggccc ggctcgagca cgcccaaggc
    16801 cgcgaccgcg gcgattttcg ccgcggcgtt cgccccgtcc tcgaagtaag ccgggaggcg
    16861 ccgcgccgtg gccagggaga agacgcgctc ccgctccata acgcattcga cgctccggag
    16921 atagggaacg gggtttccct cgaagatctc ctcggcgttc tccgtcgtgc cgccgcacgt
    16981 gctggtgtac agggcgttga tgaggccgcc ccggtagacg gccacctcgc cctcggtttc
    17041 ctccacggcc cgcgtgctcc gcggactctc gacgctcatt ccctcgtaga cctgcgactg
    17101 gggagtggcc aggagatcga atcccaggtc gccgtactgg ccgaggtttt tcaaggcata
    17161 cgtccgggcg gccacggctt gggctttcaa ggcttcgatc tctccgaaca tgtagggcga
    17221 aagctctccg ggcacgacgc ccttgaggta atcctccagg ttgagaacat tgaccagaag
    17281 ggcgccgcgc gcgcttcctc ggaggatgaa tatgcctcga tagttccggc cgttgtaagt
    17341 tagatagctc tggggattgg aggggatgag atagagggcc gaccccttgt tcaaatcaac
    17401 cagccggccg ccgatcatga cccattgcgg cttggacgcg ccccggggga cgtcttgccg
    17461 gatgatccag gcttccggat aaccgagagc ggcgagattc ctgataaagg aaagggcttc
    17521 gccccgggtg aggaaatccc ccaccaggac ctggtagacg ccctccaact tctcggcctt
    17581 ccccccggcg acggtgaccg aacggtcgat ccggaggcga agctcggagg cgatcttctc
    17641 cgcttcctcc cggcgtcggc tctgggcggc ctgaatcaag aatttttcgg tcagcgcctc
    17701 cttgtggccc ttaaccctgg cctcggcgac gtcctcggcc aggagcttgt aggaatccgc
    17761 ggcctggtag attttcatcc ccgaggaagc gtggatcaag gcgtcttcca agtcgatgtc
    17821 gagagcgatc cggatcaccg gtttttcgat caggaaacct tgaaagaagg ccgcctcttg
    17881 ggcgaattcg atcggccggc cggccagaaa aaagaggacc agaaagatca tgaaaaaata
    17941 tttatggttt cggcgcagga tttgagacat ggcggtgggg ttttatgctg ggaggtgttc
    18001 ctccgttaag ttagcgcaaa agggccggct ttgtcaagca aaaaaatcaa tccctgctac
    18061 ccggccgccc ttccctacta tatatagggg acgcggaggc gcccgtacgc aacgctatat
    18121 ctttgagatt ctcattattc tcgttttttc cctccgtccc cgcctatcca ggactcggtc
    18181 tccgccgggg gccgcaaatc cgcgcccgtt ttgacatttg atgtgatttc gctataattt
    18241 cgactccgcc aatcctcgat taaaagggtg aacatgtcga acatagggaa ttacgaagag
    18301 cgctgcgaga ctttcacatg ggaacaagct gaacaagagc tcgagtaccg gcccggcggc
    18361 gtcctcaata tcggttggca ttgcaccgac cgcgtctgcc tccagggtca gggggataaa
    18421 acggccctca tttgggaggg catgggcggg aacgagaaac gctacacctt ccatgacatc
    18481 cgccgggcct cgaacacgat cggggcgttc ctccgcggac tcggcctgca gccggaggac
    18541 cggatctgcc tgttcatgga caagatcccc gagctgtatc tcggcgtctt gggcatcctc
    18601 aagatcggag cgatcgccca gcccttgtat tcggccttcg gcgacgaatc gctgttcgtc
    18661 cgtctcgaaa acgcccaaac gaaagccgtc atcactcaga aaaagcacgc ccccaaggtc
    18721 cgaaaaatcc tcgacaagct tccccacctt cagcacttga tcatcgtcga tcacgacggc
    18781 aagccgttga aggagcggga ggtcgctttt tccctggaaa aatccgcccc cgtggaaacg
    18841 ctcgacattc atccgacgtc ggccgaaagc ccgtcccttc tccactacac gtccgggacc
    18901 accggccttc ccaagggcgt caagcatgtc cattactccc tcctatccca gtacatgacc
    18961 gccaagtggg ccctcgacat ccgcgacgac gacatcttct ggtgcacggc cgatccaggc
    19021 tgggtcacgg gaacatccta cggggtgatc ggcccctggt gcctcggcgt cacccaatgc
    19081 gtcctggact ccggattctc ggccgaagcc tggtaccggt tcatcgagaa aaatcggatc
    19141 accaactggt attcggcccc gacggccatc cgctcgctga tgaaagccgg cgaggaggtc
    19201 gtccgtaaat acgacctctc gagcctccgg gccttgtcca gcgtgggcga gccgttgaac
    19261 tcggaggcgg tcctctggtc ggagcgggtc ttcggcaagc ccttcctgga cacgtatttc
    19321 caaaccgaaa cgggctccat catgatcacc aacttccagg ggatgaaaat caagccggga
    19381 tccatgggca agccgttccc cgggatcacg gccgtcatcc tggaccagaa aacattcgag
    19441 ccctacgcgg aaccgggaaa aatcggcctg atcgccctca agcccggctg gccggccatg
    19501 atgcggatgt actggaacaa cgaggagacc taccggaaga aattcaacaa cggctggtac
    19561 ttgaccggcg accgggccag catcgacaag gaaggctact tctggttcgt cggccgcgac
    19621 gacgacatca tcaataccgg cggccatctc gtcagcccct tcgaggtcga atcggccctc
    19681 atcgagcacc cggccgtggc cgaatcggcg gtcgtcagca agccggacac gatcaacatg
    19741 gaagtcgtca aggcgttcgt caccctgaaa gccggcttca cccccagcgc cgatctcgac
    19801 ttggaaatca tgaacttcat ccggaaaaag ctctcccccc tggccatgcc ccaggagatc
    19861 gaattcaccg aggccctgcc caaaacccgg agcggaaaga tcatgcgccg gatcctcaag
    19921 gccaaggaat ggggcgagga gatcggagac acctcgacgc tggaaaacga ctgagcatcc
    19981 ggcggccgcg gggcgcgccc cgggcctgga catattcacc gagaaggagc gaccacatgt
    20041 ccgacgaact caaagacatt gtcctgaagt acgtcatcga cgaatatctc gaggacgact
    20101 ccgagccgat cacgtacgac acgcccctga tttcgggggg gatcgtcgat tcgttttcga
    20161 tggtttccct caagcgcttc ctcgagaaca aatacaagat ttccattccg gacgaccagg
    20221 ccacgccgga ggcgttcgac tccgtcgaca agatcgccgt gctcgtcaag aaattcacca
    20281 aggcctgagc cggccgccgg cgccgccctc ccctcggatg gaaatctccg accggccggg
    20341 cgggccgtcc gaatcccgcg gaaaaggaga aacgtcatgt cctattcgga aaaaacccgg
    20401 gccgcttacc aggaggtcct ccagggcctg aaggacgccg gccttttcaa gcaggaacgg
    20461 tttatccatt cctcccaggc ggccgacatc gaggtcgaat tcccggccgg cgcctccgtc
    20521 cgcaaagtca tcaacatgtg cgccaacaac tatctcgggc tgtcgagcca tcccgcagtg
    20581 gtccaggccg cccacgaggg catggacgcc cgcggctacg ggatgtcgtc ggtccggttc
    20641 atctgcggga cgcaggacat ccaccgggac ctcgagcgga aggtcaccga gttcctcgga
    20701 accgaagaca cgattctctt cccttcgtgc atggacgcca acgccggcgt cttcgaggcc
    20761 gtcctggcca aggacgacgt catgatctcc gaccggctcg tccacgcctc gctgatcgac
    20821 ggcatccgcc tttgcggcgc catgcaggac acgtacaagc acgccgacat ggcccatctc
    20881 gagcagaagc tcgaaatgca ccaggaccgg cgcttccgga tggtcatcac cgacggcgtt
    20941 ttcagcatgg acggggacac ggcccccctg gacaagatgg ccgctctctg cgacaagtac
    21001 gacgccatga tcctggtgga cgattcccac gccaccggct tcatcggccg gaccggccgg
    21061 gggacccatg agaaatacgg cgtcgtggga cggatggaca tcatcaccac gacgttcggc
    21121 aaagctctcg ggggggcgac cggcggctgc gtctccggcc gccgcgagct ggtcgaaatg
    21181 tgccggcaga aagcccgtcc ttatcttttc tccaactcga tcgccccggt cgtcgttcaa
    21241 ggcatcctca aggtcttcga catcctcatg gcctcgacgg agcgccggga caagctcgaa
    21301 aagaacgccg cctactggcg gaaaggcctc caggaggccg gattcgtcct caaggaaggc
    21361 gacacgccga tcgtgccggt catgctgttc aacgccaagc tggcccagga cttctcccgg
    21421 gccctctacg aggagggcat ttacgccgtc ggcttcttct tccccgtcgt tccccagggc
    21481 caagcccgca tccggaccca gatctcggcg ggtcatgaaa tccaccatct cgacaaagcc
    21541 ctcgaggcct tccggaaagt cggaaagaca ttcgacatcc tgggccggac caagcaggaa
    21601 atcatcgaac gtttcggaat gtaaaagaga gcgccgcgca tggacaacaa actcgccaat
    21661 cccgccccgc tcggcttgat gggcttcggc ctgaccacgg tcctgctgaa catccacaac
    21721 gccggcttct tccccctcag cgccatggtc ttggccatgg gcatctttta cggcggcctg
    21781 gcccaggtca tcgccggcat catggagttc aagaagggca attccttcgg aacgacggcc
    21841 ttcacgtcct acggcttgtt ctggctgacc ctggtcgcga cctgggtgat cccgggggcc
    21901 aacgccccca acggcggagc cacgccggcc ccctacctgg gctggtatct cttcctttgg
    21961 ggcttattca cgttcctcat gtggatcgga accttcggca agaaccgggc cctccagttt
    22021 gttttcctga gcctctggat ccttttcctc ctcctggcgg tccgggattg gacgggctcg
    22081 gaaacgatcg ggaaaatcgc cggcttcgag ggaatcgtct gcgggctgtc ggccgtctac
    22141 ttggccatgg ccgagatcat caacgaagcc cggggacgga cgatcctgcc gatcggcgcg
    22201 tccaaggcct gaagcaagaa tatttttcag cgcctttccg gcggcaaaaa aaaatcaagc
    22261 ggcgattaga tttttcgggc cgtcccccgc actcttttta gccgccgtaa catcctgata
    22321 agaaaataat tacttgactt tttcttttcc tcgtcttatt ttctaatcac gacggacaga
    22381 gagggaaaag gacactcctt ttttgagatt cggacaaatc aacggcggac ttttctccat
    22441 tccctagcgc gtgaggaact atgtccgggc ggctccggcc cccggctatt tttctttaag
    22501 gaggagggaa agcctatgaa aggcaggcga ttggacagac tcttgacggc cgtcgccgcg
    22561 gcggccgccg cgaccgtcct ggtgttggtt ctcgcggcgg ggggacaaac cccggccggc
    22621 ccgggatccg atacttcggg cgtcggagcc tatatccccc gtcagcttgt cgtcttggcc
    22681 aagacgcctc aagccgacaa agacgtcaaa gccctggcca acgcgctcgg cgcggtcatc
    22741 caagccgaga ttccgcaagg ccggctgttc cttttcgtct tcagcgatga tgccaccgcc
    22801 ggtaacgccc tgctgaagat caagaaggac aacaaagaaa gcttcgacgc ttggcggaac
    22861 tggaaattct ccattcccaa acccgcccgg ccgccgaaga aaacgcatgc cggcgcggcc
//
LOCUS       DAOOXH010000034        21472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_2298_length_21472_cov_8.398795, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000034 DAOOXH010000000
VERSION     DAOOXH010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 21472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21472
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2298_length_21472_cov_8.398795"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1609)
                     /locus_tag="PKV64_05650"
     CDS             complement(194..1609)
                     /locus_tag="PKV64_05650"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPN16600.1"
                     /translation="MSNQVWDRMSKSLEGIVNIEETRVTLLDEKRLPETMDGLIQEAV
                     FGDPDVQAAARWLIRETAQAAGIRPASIHELYVARGRGQIEKAFTVPAMNLRMLAYDS
                     ARAVFRAARTIDCGAFIFEIARSEMVYTDQRPTEYVAAVLAAALREGYRGPVFIQGDH
                     FQAGAKKYAIDPEAEVQAIRDVIVESIGAGFYNIDIDTSTLVDLSLPKLHDQQKKNFE
                     LCATLTRFIRDREPAGVTISIGGEIGEVGHQNSTVEDLDAFMEGYNAARGAKTGISKI
                     SIQTGTSHGGVVLPDGSLADVAIDFEALRKLSARAREKYGMGGAVQHGASTLPDTAFH
                     RFVEAGTCEVHLATAFQTLIMDHPTVPESLRLEMYDWLKKNAAAERKPKDSDAQFFYK
                     LRKKAVGPFKKRFWDLPREAREEIGADLEKKFQYLFEQLGMARTTGIVKRFIKAPEIH
                     TPRPAGGIRKAKTENVDGLAD"
     gene            1836..4178
                     /locus_tag="PKV64_05655"
     CDS             1836..4178
                     /locus_tag="PKV64_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HPN16601.1"
                     /translation="MSHRMLRILAAAALSAVLTALPSTAAERPVVTAADYARAERFLG
                     AQAASLVFRASVEPHWLSGDRFWYRNRLAGGSEFILVDAPRGVRGPAFDHVRVAAALS
                     AAEGKPHAATSLPFNEIEFMDGGKRISFVVGARRYSCDLRGRKMTFEAIAPPGGRPEK
                     EREPGAASPDGRLEAFLRGDNLWVRETETGTERPLTFDGVKDFGYATDNAGWSKSDRP
                     IVAWSPDSRKIATFQQDQRGVGEMPLVEVKAGRPVLHVWKYPLPGDETVITIQRVVVH
                     LDGPRVVRLKVPPDARRGSVTDDIQASDGQMEDVAWSRDSRRLAFVSTPRDHSSALLR
                     VADPETGEVRDVFEETTKTFFESGAGESAWRFLPESGEFIWFSEREDWGRLFLYDLET
                     GRLKNPITSGPGRAARVVRVEPEKRRLTFAGLGMESGRDPYFRHIYQIGFDGTGKVLL
                     TPEDMDHEVVYSPSGRYLIDTFSRPDVPPTTVLRTAEGRFVLTLEKADISALLETGWT
                     PPTPFTVKARDGVTDLYGLMFRPTGFDPARKYPILNNIYPGPQSGSVSSRSFSASRRW
                     DARAIAELGFVVVQIDGMGTPNRSKKFHEAYYGNMADNTLPDQVAGMKELAVRYPWID
                     LDRAGIYGHSGGGYAAAAAMFHYPDFFKVGVSQAGNHDNRGYEDDWGEKWHGRLVRRP
                     DGTSNYDSQANQNFAANLKGKLLITHGTMDDNVPPYLTLLVVDALIAANKDFDMILFP
                     NRGHGFGSEPYMTRKRWDYFVRHLLGAEPPREFVFKPRPK"
     gene            complement(4238..5824)
                     /locus_tag="PKV64_05660"
     CDS             complement(4238..5824)
                     /locus_tag="PKV64_05660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HPN16602.1"
                     /translation="MKRAALGVLFLAALAAAFFFRLTSLDLRPMHHDEANQAVKFGAL
                     LERGEYRYDKTDHHGPALYYLTLPAAKAAGRAKLADLDEMLLRTVPAVFGGALLLLLL
                     FFGRSLADEARALAALFLAVSPAMTYYSRFYIQEMCFAFFVLGFLVSLWRYLVRPDGE
                     GSLFAGVFAGLMAATKETAVIVFAAAALGVAAVLTRERLIAPKSDTPIRRRFFARHFI
                     ILLIVAAAVAAAFFSSFGSHPAGIKDAADALKIYVEKGSAAPGFHGHPFFYYLGILAG
                     SASGGLVWTELLVLLLGAAGMVFVFLPHRRSFLDEIPDPLPVDRSLGLFLVVYTLAAT
                     LAFSLLGYKTPWNMIPFYLGWLILAGIGGITLFRMMKTLPLRAAVLLVLAAGLGHLAG
                     QSRLACVRYPADPRNPYVYAQTSPDFLKLVGRVEALAAVHPEGRNLLVKVVAEPYEQW
                     PLPWYLREYKTVGYWTEAEAAGNLGAADLVIASAERAEDVENALGRPSIVEHYGLRPG
                     VLLTLFIPPGLWEAFMKSRG"
     gene            complement(5821..6501)
                     /locus_tag="PKV64_05665"
     CDS             complement(5821..6501)
                     /locus_tag="PKV64_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008100199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16603.1"
                     /translation="MKSYAPAAALAAALLILVPVYASLSAQDSDKEELKLKLPKPMFI
                     GTPRNIKTANLETITGKSRGPFFVPKGTRLLSAGKAVLSSDMQPVIGDLGFVVDGEKS
                     GEDGYFVELGPGRQWVQIDLGKTAALHAILIWHYHSQARVYRDVVVQASDDKDFRGNV
                     LTVYNNDHDNTLGFGPGKDKEYIETNEGRLIDPKGAAARYLRLYSNGNTSNDMNHYVE
                     VEVYGILK"
     gene            complement(6594..7532)
                     /locus_tag="PKV64_05670"
     CDS             complement(6594..7532)
                     /locus_tag="PKV64_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPN16604.1"
                     /translation="MSAPPSAVPRFAHEAMTTLFEIFIPGPDEIYAGQAAREAFREID
                     RLEARFNRFDPGSEISRINRLLPGEETTVGIETFECLALAEAVRLETGGAFDANARAA
                     ALDSFAEGRDAKPEPAPFELLGGAGSCLVRRLEEERPGHGPIDLDLGGIGKGYALDRA
                     GQLLLTWGVPDALLHGGTSTVLALGSPAAASGTEPSRESSERTGWPVGIGAGWPGAPE
                     RVFLSGRALSGSGTEVKGGHILDPRTGRPARGNLAAWASAPTAALSDALSTAFFIMTA
                     DGVEACAARNPEIGACLVVAYGDVRIFNPRFFPAGG"
     gene            complement(7529..8227)
                     /locus_tag="PKV64_05675"
     CDS             complement(7529..8227)
                     /locus_tag="PKV64_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16605.1"
                     /translation="MTVFVILAAVLPAALVLVLTARRFASSIPAAEPAEERRRPAPGA
                     YLGWLRMRLKALAVPSFFRKTWDLWNGWAEARYRGWTKWIFLAWAAAFLYLAGSGLFF
                     AIFVRRGLYGLPLLGHVMSGGLFAAGLAAVLILRAGAHGFDRREETGPAAFAVLFVGT
                     ISRAIVRKTVFWWFAAFGFVQVTTALASMLPMFTFNTQVALLMIHRFSALGLTLAAIV
                     FFDLILPDRAGPRA"
     gene            complement(8234..8554)
                     /locus_tag="PKV64_05680"
     CDS             complement(8234..8554)
                     /locus_tag="PKV64_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16606.1"
                     /translation="MAHEVRPARQALGWNGCTDCHSLSSKFFFARVDGQGPLRTEKIS
                     RRPASSYMGVDNLYHRLFGLSYAGRPYFKILLGAAALVIGAVLLAALVLAAGRLSGLI
                     EKRK"
     gene            8546..8722
                     /locus_tag="PKV64_05685"
     CDS             8546..8722
                     /locus_tag="PKV64_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16607.1"
                     /translation="MRHRPSHRIGGRIVERFEAVFLVEAEKLAPRDINGIPPAAAGQG
                     FQDKGNLRFGQAPF"
     gene            complement(8719..10701)
                     /locus_tag="PKV64_05690"
     CDS             complement(8719..10701)
                     /locus_tag="PKV64_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16608.1"
                     /translation="MSAVKPSRFSGRARAFAGPVLAAAAAVVFILAPAEAETPPKWAG
                     SRTPAVHRIPLNDEFGQKIIPSEPNPLPFSARTTCAPCHDYETVKTGRHFNAASPDAP
                     AGRPGEPWIWLDERTGTQIPLSERSWKGVFKPADLGLSSWDLTLLFGRHFPGGGPAEP
                     EPEDETPDSRWSVSGKAEINCLGCHSASGIQNHSEWAKQILRENLRWAATAASGLGEV
                     GGIASRLAGTWDIADGPNPDDSEWAVAPFVKYDRALFDSKHQAFLDIAYPPRDSSCLA
                     CHSAAPAGSRKFDYEGDVHGQAGLACASCHRNDLSHRMIRGYEGEAEDDPALLSADFT
                     CAACHLGREKDQNDPPLPGRMGAPYPLHKGLPEVHIERLACTVCHSGPLPEKEAVRVR
                     TARANRLGIFGIADWSTDLPAIVEPVYLRDARGKLTPHRMMWPAYWGVKEGGKKTGPV
                     RPLRPAEVEAAAGDILYPDRTAVRILAALQNASGLTGKAVLFAGGKIYEPNLDGGLTA
                     VVPEAGTAVPAGDGFAALENGLLTPFVPDFNPADSETAAEAEGAIQTALEALLLTEAA
                     PGEPALRFKGFLYRLVDGFLDKMENAEAAGTGPEWLWAAGETLRPMLSEADKNAVLTL
                     TGTDRRLTETQVSLVLEALAKGNLHFGQAPVRSGER"
     gene            complement(10706..12088)
                     /locus_tag="PKV64_05695"
     CDS             complement(10706..12088)
                     /locus_tag="PKV64_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008102410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN16609.1"
                     /translation="MTDFGSKKNDPAVSRRSFLKTSAAAGLGAAVGGALLARPSPDPA
                     SSQAESPGRPGDLPVALIGAGEQGRVLMESCLRIPGIRIAAVCDIWSYSQQYARGYLR
                     KYGQDPAVYEDYRDLLAKEKGLAAAIVATPDWLHAEQTNACLENGLHVYCEKEMSNSL
                     EKARTTVETARRTGRLLQIGHQRRSNPRYLHAIDRLIRDKKLLGRVGLAYAQWNRSKA
                     DLLGWPKTYPIDPAKLQAYGYADMTEFRNWRWYKKFGGGPIVDLGSHQIDLFAWVFGC
                     NPSAVFASGGVDYYKGREWFDNVMAVYEFQTAEGAARAMYQVQTTTKYGGFYEVFMGD
                     DGAIQISEVAQRGNWALREAHAPEWDSLVKEGLLLSEAPVIQKVDTKNIFVDVRVTAE
                     AGRWPFPVDLAKPAHQPHLENFFDAIRRGTALTCPASLAYESAVAVLKVNEAVRTKSL
                     VRFRPGDFKA"
     gene            complement(12252..12770)
                     /locus_tag="PKV64_05700"
     CDS             complement(12252..12770)
                     /locus_tag="PKV64_05700"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HPN16610.1"
                     /translation="MKKTVLFILLAAAGAGFGLGQDVVVKDQTPFVYAYIECTGSYSL
                     ISAKIGEFMNEYFSQGLGSFTGVMAMYLNAPGEVPEAELKWRIGMPIGADVTPQEPLR
                     KDEFAYPKVASCLHVGPYDKVGEAYQKIGAYLERNGWKAAGPPVEFYLNDPGQVPPEK
                     LETEILIPVEKK"
     gene            12935..14779
                     /locus_tag="PKV64_05705"
     CDS             12935..14779
                     /locus_tag="PKV64_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIA30 family protein"
                     /protein_id="HPN16611.1"
                     /translation="MKKTAIAVLGAAVLIGASVRASEPVPGPAGNEAGSTAFINVRIF
                     DGEKVIPAATVVVEGGRITAVGTEAAVPAGAEIVDGAGRTLLPGLIDSHVHIWDESGL
                     REAAVFGVTVLVDMFTSAEFVSAQKKAQAAGFRPMAHLVSSGILATAPGGHGTEYGVK
                     VPALTAPGEAQAWVDARLAEGSDFIKIIRDDGSTYGRAIPAIARETEAALIAAARARG
                     RRTVVHAATLRQCREALEDGADGLAHLHFDDAFDPDFGRLAASRKAFIIPTLTVLAAM
                     NGSPDPAGVASDAGLSPYLRPEDLQMLGQTMPFRSAPGAYAAAERTLRELRTAGVPIL
                     AGTDTSNPGTAFGAALHGELALLVKAGLTPLEALRSATSIPAALFDLGGRGRIAPGSR
                     ADLLLVEGDPTVEIKATRAIVGVWKDGLRIDRESWRSAAAEARRARENQKNAPAPEGL
                     GDGLISDFEDGAVSARFGSGWTASTDSLMGGKSRAELSTVDGGAEGSARALKIEGEIS
                     ETGSIRWASALFSPGRAPFTPANLSSKKALSFWARGEGKTFAVMIYAQSSGFIPKIAF
                     FPAGPEWKEVVLPFEKIGLEGFDILGIAIGATTAPGPFWLEIDDVRLK"
     gene            complement(14837..15469)
                     /locus_tag="PKV64_05710"
     CDS             complement(14837..15469)
                     /locus_tag="PKV64_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16612.1"
                     /translation="MRSRLKTAILAALVILAAGLLSSCAALEPFLSALSNLQRLQFRL
                     ADVSDFSVLGIRIEGKSALNDFSVMDGVKLVQGFTAKRLPAEFVLRVEVRNPNDGTGG
                     SPRTVSTLTGLESRLLIDGTPTVIGNIDQAIEIPGTGRNTVIPIRMTIDLYEFFAAKG
                     YEGLLNLALALGGAERDPARVALDAQPTVTTPLGPIVYPGRITIIDAEFR"
     gene            complement(15496..16281)
                     /locus_tag="PKV64_05715"
     CDS             complement(15496..16281)
                     /locus_tag="PKV64_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPN16613.1"
                     /translation="MALNLQGKTILVTGASSGLGLALSRLLAEENVNLALLARRDDVL
                     RRTAAELQHSGSAVLPVKCDVSKREEIQAAYREVRSRFGGVDIAILNAGVSGRGGMNL
                     FDVGAARTMFDVNVFGVVDFLGELLPDFKKRGSGTIVGVSSLSDSRAVPRNGFYCASK
                     SAVSLILDALRIELKPRGIKVVTVKPGFVKTPMTDGNDFKMPFIVSAEKAAAVIVKGL
                     KKEKTYIRFPFLMRVSAWVLKLMPNFLYEIFAARVKSGAENKK"
     gene            complement(16283..17518)
                     /locus_tag="PKV64_05720"
     CDS             complement(16283..17518)
                     /locus_tag="PKV64_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPN16614.1"
                     /translation="MFRKKTILGLLAASLLLAASAAAQDKPKDKGIFIEPKNPFWEEI
                     ARTTGEFGKARPAPPRRTFMVDLAGFAVPKSLDGFKTAWHNPPVSQGNTNTCWSFATT
                     SFFESEIYRIHGKKVRLSEMFTVYAEYLERTKRFIRERGDSYVAEGSEGNAVTRNWKI
                     YGCMPWEAFSGLKPGQKFHDHSKLIEEVKAYLDHVKTAGAWNEAAALETVRAILGAHM
                     GTPPEKFTVEGREYTPRTYLRDCLKINPDDYVDVVSYLQQPFGPNVEYEVPDNWWHSK
                     DYVNVPLDTFMKILKKGLAAGFTMSIGGDISEPGKNAEKKVHMIPTFDIPAEFIDDNA
                     RQYRFGNGTTTDDHGIHLIGYKEDGGRLWFLAKDSGSSAFNFDPKGYYFLTEEYLKLK
                     IMDFMVHKSVLEGLVEGSR"
     gene            complement(17813..18037)
                     /locus_tag="PKV64_05725"
     CDS             complement(17813..18037)
                     /locus_tag="PKV64_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPN16615.1"
                     /translation="MGGEELRNTIRVQRAIKDWTQEELARRVGVTRKTINTIENAVFV
                     PSTVLALKIARAFGVKVDDVFQLADAPPES"
     gene            complement(18039..18494)
                     /locus_tag="PKV64_05730"
     CDS             complement(18039..18494)
                     /locus_tag="PKV64_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16616.1"
                     /translation="MTPASKIDEFERTYRGVYSRFVIVFLVFSAAWLVRFIVRTIGTL
                     PEWLDIGFAVILILTLPAQFYSVVRLSALRKRGQADAELGVLFTDERIQAHGKAAWMY
                     GFIAMASVLAVLGVISVFADLKDTNAVIFTALWAGFGAYHLSFVLMERR"
     gene            complement(18864..20174)
                     /locus_tag="PKV64_05735"
     CDS             complement(18864..20174)
                     /locus_tag="PKV64_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HPN16617.1"
                     /translation="MMETKRPRKNALLYGAAFLATAAVIAGVLALLFNVRTRREEAYR
                     YPLKVVEIKDGEIDPAVWGKNFPLEYDTFVRTRENYGRTFHGGSERFSRIDEIPAAKR
                     LWAGYAFSVEYNEERGHFFALTDQKMTRRVREFDQPGACANCHSGKAPALIREMGWEA
                     FNKTPYKELSPKLRHGSVCADCHDPKTMELVITRPAFKKAMAAAGVDLAKATRQDMRT
                     YVCAQCHVEYYFYGENKELTFPWAKGRTIEAIEACYDENGHVDWVNAETGAAMLKVQH
                     PEFETWGTGLHAKSGVACADCHMPYVRAGSVKVSDHWIRSPLTNIAAACQACHPRPEE
                     ELKSRVKDIQDKTAGLLRKTETALLAAMDAIIKAKAAGVPDERLSASRALIRRGQLRW
                     DFVFSENGTGFHSPQEAARVLAEAIDFARQAELAAALLAPAGIR"
     gene            complement(20191..20667)
                     /gene="nrfH"
                     /locus_tag="PKV64_05740"
     CDS             complement(20191..20667)
                     /gene="nrfH"
                     /locus_tag="PKV64_05740"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HPN16618.1"
                     /translation="MNGPEQTTEPTRRRRRGPAVLLFALAGAAAGLGGFTFFFAQGSS
                     YLKDDPKICMNCHIMRSHFDDWNRASHKAVAVCNDCHTPPGFLGHWTVKGINGFNHGK
                     AFTTGDFHEPIRIRPLNKRVLLDNCRRCHEPVIERMRTNSRGEEADCSRCHRNAGH"
     gene            complement(20701..>21472)
                     /locus_tag="PKV64_05745"
     CDS             complement(20701..>21472)
                     /locus_tag="PKV64_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPN16619.1"
                     /translation="LKSAADLLAAGDWAGLKERFGTLASAVKKMMFLEERILFPTSAR
                     KLKTSDWVRIKNGEPAIGYAWIKPSAVWDARLAESMSAPAAEPAPSAPAAPSRGAEKL
                     SAGGEGPAASGIGPALETIRLSQGRLTPEQIDLMLRRLPVDITFVDENDRVAYYSDGP
                     ERIFPRSPEVIGRKVQNCHPPKSVHIVNEIVQAFKEGTKDVAEFWIQREGGPFIHIRY
                     FAVRDEAGSYRGTIEVSQDIAPLRALQGERRLLNWEML"
BASE COUNT         3752 a         6543 c         7281 g         3896 t
ORIGIN      
        1 gcggcatttg cgaagaggcg acaaatgccc cgctccgggg acgtgcgccc gctttccggg
       61 aggcagagag tcttggattc agcctgggct tcagtcccgc tccgcggcat ttgcgaagag
      121 gcgacaaatg ccccgctccg gggacgtgcg cccgctttcc gggaggcaga gagtcttgga
      181 ttcagcctgg gcttcagtct gccaggccgt cgacgttttc ggttttggct ttccggatgc
      241 cgccggcggg ccggggagta tggatttccg gcgccttgat gaagcgtttc acgatgccgg
      301 tggtccgggc catccccagc tgttcaaaca ggtattggaa tttcttctcc agatccgctc
      361 cgatctcctc ccgggcttcc ctcggcaggt cccaaaagcg cttcttgaac gggccgacgg
      421 cctttttccg aagcttgtag aagaattggg cgtcggaatc cttgggcttg cgctccgcgg
      481 ccgcgttttt tttcagccaa tcgtacattt ccagccggag cgactcgggg acggtcggat
      541 gatccatgat cagggtttgg aaggcggtgg ccaggtggac ttcgcaggtc ccggcctcga
      601 cgaaccggtg gaacgccgtg tcgggcaggg tgctggcgcc gtgctggacg gccccgccca
      661 tgccgtactt ctcccgggcc cgggcgctca gcttccggag ggcctcgaaa tcgatggcta
      721 catcggccaa cgacccgtcg ggcaggacca cgccgccgtg cgatgtgccc gtctggatgc
      781 tgattttgct gatccccgtc ttggccccgc gggcggcgtt gtacccctcc atgaaggcgt
      841 cgaggtcctc gaccgtgctg ttttgatgcc cgacctcgcc gatttcgccg ccgatcgaga
      901 tcgtcacccc ggcgggttcg cggtcgcgga tgaagcgggt cagggtcgca caaagctcga
      961 aatttttctt ctgctggtca tggagcttcg ggagggagag atcgacgagg gtggacgtgt
     1021 cgatgtcgat gttgtagaac cccgcgccga tggattcgac gatcacgtcg cggatggctt
     1081 ggacctcggc ttcggggtcg atcgcgtatt ttttggcccc ggcctggaag tggtcgccct
     1141 ggatgaagac gggcccgcgg tatccttccc ggagggcggc cgcgaggacg gcggcgacgt
     1201 actcggtcgg gcgctggtcc gtgtagacca tctcgctccg ggcgatctcg aagataaagg
     1261 cgccgcagtc gatcgtcctg gcggcccgga acacggcccg ggcggaatca tacgccagca
     1321 tcctcaagtt cattgccgga acagtgaagg ctttctcgat ctggccgcgg ccccgggcga
     1381 catacagctc atggatcgag gcgggacgga tgccggcggc ctgggcggtc tcccggatca
     1441 gccaccgggc ggcggcttgg acgtccgggt cgccgaaaac cgcttcctgg atcaagccgt
     1501 ccattgtttc cgggagtctt ttttcatcga ggagggtgac gcgggtttct tcgatgttca
     1561 cgatcccttc cagagatttc gacatgcggt cccaaacctg attggacatg atcggctcct
     1621 tgaaacggct ccgggttttc cgggcgttga gtcgccccga ttatatcaaa acgggcccgg
     1681 tcctggcatc ttcatccttg tccctttgga acgattgatc ttctcgggca aaacagccac
     1741 aatagcaagg atttccggac ggccctcctg ttttcagccc gggccgtcgc cgagaccgtg
     1801 gctatgatcc acgatcgata aaaaggaggc tgccgatgtc ccaccggatg ctccggatcc
     1861 tcgccgcggc ggcgttgtcc gccgtattga ccgcccttcc ctcgacggcc gcggaacgcc
     1921 ccgtcgtcac cgccgccgat tatgcccggg cggaacgatt cctcggggcc caggccgcgt
     1981 ctctcgtttt ccgggcttcc gtcgagccgc actggctttc gggagacagg ttctggtacc
     2041 ggaaccggct ggccggagga tcggaattca ttctggtcga cgcgccccgg ggcgtccgcg
     2101 ggccggcgtt cgaccacgtc cgggtcgcgg ccgctctgtc ggcggccgag ggcaagccgc
     2161 acgccgcgac gagcctgccg tttaatgaaa tcgagtttat ggacggaggg aaaaggatct
     2221 ccttcgtcgt cggggcgcgc cggtattcct gcgatctccg cggccggaag atgacgttcg
     2281 aggcgatcgc cccgcccggc ggccggccgg agaaagagcg tgagccggga gcggcgtctc
     2341 ccgacgggcg cctggaggcc ttcctccgcg gcgacaacct gtgggtgcgg gaaacggaga
     2401 cggggacgga gcggccgttg acgttcgacg gagtcaagga tttcggctac gcgacggaca
     2461 acgccggctg gtcgaaaagc gaccggccga tcgtcgcctg gtcgcctgat tcgcggaaga
     2521 tcgccacgtt tcagcaggat cagcgcggcg tgggcgaaat gccgctcgtc gaggtgaaag
     2581 ccggccggcc cgttcttcat gtttggaagt atccgctgcc gggggatgag acggtgatca
     2641 cgatccagcg ggtcgtcgtc cacctggacg gaccgcgggt cgtccgcttg aaggttccgc
     2701 ccgacgcgcg ccgcggcagc gtgaccgacg acatccaagc cagcgacgga cagatggagg
     2761 acgtcgcctg gagccgggat tcgcgccggc tggcgttcgt ctccacgccg cgcgaccact
     2821 cctcggccct gctccgggtc gccgatcccg agacgggcga ggtccgggac gtcttcgagg
     2881 aaacgacgaa gacatttttc gaatccgggg cgggggagtc ggcctggcgg tttttgccgg
     2941 agtccggcga attcatctgg ttttccgagc gcgaggattg gggacgcctc ttcctttacg
     3001 acctcgaaac cgggcggctg aaaaatccga tcacctccgg ccccgggagg gccgcccgcg
     3061 tcgttcgggt cgagccggag aagcggcggc tgacgttcgc cggcctgggg atggaatccg
     3121 gccgcgatcc gtacttccgg catatctatc agatcggctt cgacgggacc ggcaaggtcc
     3181 tgctcacgcc ggaggacatg gatcacgagg ttgtctattc gccgtcgggc cgctatctga
     3241 tcgacacgtt ttcccgcccg gacgttccgc cgacgacggt cctgagaacg gccgaaggcc
     3301 ggttcgttct gacccttgaa aaagccgata tttcggccct cctcgagaca ggctggacgc
     3361 cgccgacgcc cttcacggtc aaggcccgcg acggggtcac cgatctctac ggcctgatgt
     3421 tccgcccgac aggatttgat ccggcccgca aatatccgat cctgaacaac atctatcccg
     3481 gcccgcagag cgggagcgtg agctcccgga gcttctcggc ctcgcgccga tgggacgccc
     3541 gggcgatcgc cgagctggga ttcgtcgtcg tccagatcga cgggatgggg acgccgaacc
     3601 ggtccaagaa attccacgag gcgtattacg ggaacatggc cgacaacacg ctgccggatc
     3661 aggtcgcggg gatgaaggag ctggccgtgc gctatccctg gattgatctg gaccgggccg
     3721 ggatttacgg ccattcaggc ggcggatacg ccgcggccgc ggctatgttc cattaccctg
     3781 atttcttcaa ggtgggagtg tctcaagccg gcaatcatga caaccgcggc tatgaggacg
     3841 attggggcga gaaatggcat ggacggctcg tccgccgccc ggacgggacg tcgaattacg
     3901 actcccaggc caaccagaac ttcgccgcca acctgaaagg aaagctcctg atcacccacg
     3961 gaacgatgga cgacaacgtt ccgccctacc tgactcttct cgtggtcgac gccctgatcg
     4021 cggccaacaa ggatttcgac atgatcctgt tccccaatcg ggggcacgga ttcggcagcg
     4081 agccgtacat gacccggaag cgctgggatt atttcgtccg gcatctgctc ggagccgagc
     4141 cgccgcggga gtttgtcttc aagccgcggc cgaagtaacc ggccgtcttg gatgcgcggc
     4201 cggctgggga atccacccgg agccttggcc gcggcctcta accccggctt ttcatgaacg
     4261 cctcccagag gccgggagga atgaaaaggg tcaggaggac tcccgggcga aggccgtaat
     4321 gttcgacgat ggacggtcgt ccgagcgcgt tttcgacgtc ctcggcccgc tcggcggagg
     4381 caatgaccag gtcggccgcg ccgagattcc ccgccgcttc cgcctcggtc caataaccga
     4441 cggttttata ctcccggaga taccacggca gaggccactg ttcataaggt tcggccacga
     4501 ccttgacgag gaggttccgt ccctctggat ggacggcggc cagggcctcc acccgtccga
     4561 caagcttcag gaaatcgggg ctggtctggg cgtagacata aggattgcgg ggatcggccg
     4621 ggtagcggac acaggccagg cggctttgtc cggcgagatg gcccagaccg gccgccagga
     4681 ccaggaggac ggcggcccgg agggggagcg ttttcatcat ccggaacagc gtgattccgc
     4741 cgatcccggc taaaatcagc cagccgagat aaaagggaat catgttccag ggggttttgt
     4801 agccgaggag ggaaaaagcc agcgtggccg caagcgtgta gacgacgagg aaaaggccga
     4861 gagagcggtc gaccggaagc ggatcgggga tttcgtccag aaagctccgc cgatggggga
     4921 ggaaaacgaa aaccattccg gccgccccga gaagaaggac gagaagctcg gtccagacga
     4981 gtcccccgga ggcggagccg gccaggatcc cgagataata aaaaaacgga tggccgtgga
     5041 accccggggc ggcgctccct ttttccacgt agatcttcag ggcgtcggcg gcgtccttga
     5101 tcccggccgg atgagacccg aaggaggaaa agaaggcggc cgcgacggcc gccgcgacga
     5161 tgaggagaat gatgaagtgc cgggcgaaaa agcgtctccg gatgggcgtg tccgatttcg
     5221 gggcgatgag tctctcccgc gtcaggacgg cggccacgcc cagggcggcc gcggcgaaca
     5281 cgatcaccgc cgtctctttc gtcgcggcca tcaggccggc gaagacgccg gcgaaaagag
     5341 atccttcgcc gtccggccgg acgaggtacc gccacaagga gaccaggaat cccaggacaa
     5401 aaaaggcgaa acacatctcc tggatgtaaa atcggctgta atacgtcatc gcgggagaaa
     5461 cggcgaggaa aagggcggcc agcgcccggg cttcgtccgc caggctccgg ccgaagaaga
     5521 gaaggaggag aagaagggcg cctccgaaga cagccgggac ggtccgcaga agcatttcgt
     5581 cgaggtcggc gagcttggcc cgcccggcgg ccttggccgc gggcaaggtc aggtagtaaa
     5641 gcgccgggcc gtgatggtcg gttttgtcgt aacggtactc gccgcgttcc agaagcgcgc
     5701 cgaatttgac cgcctggttg gcttcatcat gatgcatcgg ccggagatcc aggctggtca
     5761 agcggaaaaa gaaggccgcg gccagggccg cgagaaacag aacgccgagc gcggcgcgtt
     5821 tcatttgaga atgccgtaga cctctacttc gacgtaatgg ttcatgtcgt tcgaagtgtt
     5881 gccgttcgaa tacagccgga ggtaccgggc cgcggcgccc ttgggatcga tcaggcggcc
     5941 ttcgttggtt tcgatgtatt ccttgtcctt gcccggaccg aagccgaggg tgttgtcatg
     6001 atcgttgttg tacacggtga ggacgttgcc gcggaaatcc ttgtcgtcgg aggcttggac
     6061 gacgacgtcc cggtataccc gggcctggct gtggtagtgc cagatcagga tcgcgtgaag
     6121 ggcggcggtt ttcccgaggt cgatctggac ccactgccgt cccgggccga gctcgacgaa
     6181 atacccgtcc tcgccggatt tctcgccgtc gacgacgaag cccaagtcgc cgatgaccgg
     6241 ctgcatgtcg ctggaaagga cggctttccc ggccgaaagc aggcgggtgc ccttgggaac
     6301 gaagaacggg ccgcgggatt tccccgtgat cgtctcgagg ttggctgttt tgatgttgcg
     6361 gggggttccg atgaacatcg gcttcgggag cttgagcttg agctcttctt tgtccgaatc
     6421 ctgggcggac agggaggcgt aaacggggac gaggatgagc agggcggccg ctaaggccgc
     6481 ggcgggagcg tatgacttca tcggaaactc cttcacttct taatataacc tgggaggcgg
     6541 gcgccttcaa gcgcgaacct ggattttcgt ccggattttg aacaggcgga gcctcatccg
     6601 ccggcgggga aaaagcgcgg attgaaaatc cggacatcgc cgtaggcgac gaccaggcag
     6661 gctccgatct ccgggttccg ggcggcgcag gcttcgacgc cgtcggccgt catgatgaaa
     6721 aaggcggtgg aaagggcgtc ggacagcgcg gccgtcgggg ccgaggccca agcggcgaga
     6781 tttccccggg ccggccgccc cgtccgggga tcgaggatgt gcccgccctt gacctcggtt
     6841 cccgatccgc tgagggcccg gccggaaaga aagacgcgct cgggggctcc gggccacccg
     6901 gctccgatcc cgaccggcca tcccgtccgt tcggaggatt cgcggctcgg ctccgtcccg
     6961 gaggcggccg cgggcgagcc gagagccaga accgtgcttg tccccccgtg gaggagagcg
     7021 tccgggaccc cccaggtcaa aagaagctgc ccggcccggt ccagggcgta tcccttgccg
     7081 atgccgccca ggtccaagtc gatcggccca tgtcccggcc gttcctcttc cagccggcgg
     7141 accaggcacg acccggctcc gccgagaagc tcgaaggggg ccggctccgg cttcgcgtcc
     7201 cggccttccg cgaatgagtc cagggcggcg gcccgcgcgt tggcgtcgaa tgctccgccg
     7261 gtttcgaggc gaacggcttc ggccagggcg agacattcga aggtttcgat cccgacggtc
     7321 gtttcttcgc cggggagaag gcggttgatc cgggaaatct cgcttcccgg atcgaagcgg
     7381 ttgaaccgcg cctccagccg gtcgatttcc cggaaggctt cccgggcggc ctgtccggcg
     7441 tagatctcgt ccggcccggg gatgaaaatc tcgaagaggg tcgtcatcgc ttcatgggcg
     7501 aagcggggga cggcggaagg gggcgcgctc atgcccgcgg gccggcccgg tcggggagga
     7561 tgaggtcgaa aaagacgatc gccgcgagcg tcagaccgag ggcggagaag cgatgaatca
     7621 tgagaagcgc gacctgggtg ttgaaggtga acatcggaag catggaagcc agggcggtcg
     7681 tcacctggac gaagccgaac gcggcgaacc accagaatac ggttttccgg acgattgccc
     7741 tggatatggt tccaacgaac aggacggcga acgccgcggg gccggtctcc tcccggcggt
     7801 cgaaaccgtg ggcgcccgcc cggagaatca agacggcggc caggccggcg gcgaacaatc
     7861 cgccggacat gacgtgcccc agaaggggca ggccgtagag tccccgccgg acgaaaatcg
     7921 cgaaaaacag gccgcttccg gccagataga ggaaagcggc cgcccaggcg aggaaaatcc
     7981 atttggtcca gccccggtaa cgggcttcgg cccagccgtt ccaaagatcc caggttttcc
     8041 ggaaaaagga agggacggcc agggctttga gccgcatccg gagccagccg agataggcgc
     8101 cgggggccgg gcgccttctc tcttccgccg gttcggccgc ggggatcgag gacgcaaaac
     8161 gcctcgccgt caggacgagg acgagggcgg cgggaaggac ggcggcaagg atgacgaaga
     8221 cggtcatggc ggcttatttc cttttttcga tcaggccgct gagccgtccg gcggccagga
     8281 cgagggcggc cagcaggacc gcgccgatga ccagggcggc cgctccgagc aggatcttga
     8341 aatacggccg tccggcatag gacaggccga acagccggtg gtagaggttg tcgacgccca
     8401 tgtaggacga ggccgggcgg cgcgagatct tctcggttcg aagcggccct tggccgtcga
     8461 cccgggcgaa gaaaaatttc gatgacaggc tgtgacaatc cgtgcagccg ttccaaccca
     8521 gggcttggcg ggccggccga acctcgtgcg ccaccggcca agccaccgga tcggcggccg
     8581 gatcgtcgag cgcttcgagg cggtttttct tgtcgaggcg gaaaagcttg ccccccgaga
     8641 cataaacggc attcccccgg ccgccgcggg ccagggcttc caggacaagg gaaacctgcg
     8701 tttcggtcag gcgccgtttt aacgctcacc ggaacggacc ggcgcctgac cgaaatgcag
     8761 gtttcccttg gccagggctt ccaggacaag ggaaacctgc gtttcggtca ggcgccggtc
     8821 cgttccggtg agcgttaaaa cggcgttttt atcggcctcg gaaagcatcg gccggagggt
     8881 ttccccggcg gcccacagcc attccgggcc ggtcccggcg gcctccgcgt tttccatttt
     8941 atcgaggaat ccatcgacca gccggtaaag aaagcctttg aagcggagcg cgggttcgcc
     9001 gggggcggcc tcggtcagca agagagcttc cagggcggtt tggatcgcgc cttccgcttc
     9061 cgcggctgtt tccgagtcgg ccgggttgaa gtcgggcacg aaaggcgtaa gaagcccgtt
     9121 ttcgagcgcg gcgaaaccgt cgccggcggg gacggccgtc cccgcctccg gaacgacggc
     9181 cgtcaagccg ccgtcaagat tcggctcgta gatttttccc cccgcgaaca agacggcctt
     9241 gcccgtcaag ccggaggcgt tttgcagggc ggcgaggatc cggacggcgg ttcggtccgg
     9301 gtacaggatg tctccggccg cggcttcgac ttcggccggg cggagaggcc ggaccgggcc
     9361 cgttttcttt ccgccttcct tcacgcccca gtaggccggc cacatcatcc ggtggggcgt
     9421 cagcttgccg cgggcgtccc ggaggtaaac gggctcgacg atcgccggaa gatcagtcga
     9481 ccagtcggcg atcccgaaga tccccagccg gttggcccgg gcggtgcgga cgcggacggc
     9541 ttctttctcc ggaagcgggc ccgaatggca gacggtgcag gccagccgct cgatatgcac
     9601 ttcagggagg cccttgtgga ggggataggg cgcgcccatc cggccgggga gaggggggtc
     9661 gttttgatct ttttcgcggc cgagatggca ggccgcgcag gtgaaatccg cggagaggag
     9721 agcggggtca tcctcggctt cgccttcgta gccccggatc atgcggtggg agaggtcgtt
     9781 gcggtggcag gacgcgcagg cgaggccggc ttggccgtgg acgtcgccct catagtcgaa
     9841 tttccgggaa ccggccgggg cggccgaatg gcaggccagg caggaagaat cccggggcgg
     9901 gtaggcgatg tcgagaaacg cctggtgctt cgaatcgaac agcgcccggt cgtatttgac
     9961 gaacggggcg accgcccatt ccgaatcgtc gggattggga ccgtcggcga tgtcccaagt
    10021 cccggccagc cgcgaggcga tgccgccgac ttcgcccaaa cccgaggccg ccgtcgccgc
    10081 ccagcggaga ttttcccgta ggatctgctt ggcccactcg gagtggttct ggatgccgga
    10141 ggcgctgtgg cagccgaggc agttgatttc cgccttgccc gagacggacc agcgcgagtc
    10201 cggcgtttca tcctccggtt cgggttcggc cggaccgcca ccgggaaaat gacgaccgaa
    10261 aagaagcgtc agatcccacg acgagaggcc gaggtcggcc ggcttgaaca cgcccttcca
    10321 ggaccgctcg gaaagcggga tctgggttcc ggtgcgctcg tccagccaaa tccacggctc
    10381 tcccggccgt ccggcgggcg cgtccggaga cgcggcgttg aaatgccggc cggtcttgac
    10441 cgtttcgtaa tcatggcagg gggcgcaggt cgtccgggcc gagaagggga ggggattggg
    10501 ttccgagggg atgatcttct ggccgaattc gtcattgaga ggaatccggt ggacggccgg
    10561 ggtccggctt ccggcccatt tcggcggagt ctcggcctca gccggggcca ggatgaaaac
    10621 aaccgcggcc gcggcggcca gaaccggtcc ggcgaaggcc cgtgcccggc ctgaaaaacg
    10681 cgaaggcttg acggcggaca tggattcagg ctttgaagtc ccccggccgg aagcggacga
    10741 gggatttcgt ccggacggcc tcgttgacct tcagaacggc cacggcgctt tcataagcca
    10801 gcgaggccgg gcaggtcaag gccgtgccgc ggcggatggc gtcgaaaaag ttttcgagat
    10861 ggggctggtg ggccggcttg gccaggtcga cggggaacgg ccagcgcccc gcctcggccg
    10921 tgacccggac gtcgacgaaa atgttcttcg tgtcgacttt ctggatgacc ggcgcctcgg
    10981 aaaggagcag cccttccttg accagcgagt cccactccgg ggcgtgggcc tcccggaggg
    11041 cccagttgcc gcgctgggcg acctcggaaa tctggatcgc cccgtcgtcg cccatgaaga
    11101 cttcgtagaa gccgccgtat ttcgtcgtcg tctggacttg gtacatggcc cgggccgcgc
    11161 cctcggccgt ctggaactcg tagacggcca tgacgttgtc gaaccattcc cggcccttgt
    11221 agtaatccac cccgccggag gcgaagaccg cgctcgggtt gcagccgaag acccaggcga
    11281 acaggtcgat ctggtgagat ccgagatcga cgatcggtcc gccgccgaat tttttatacc
    11341 agcgccagtt gcggaactcg gtcatatcgg cgtagccgta ggcctgcagc ttggccggat
    11401 cgatgggata ggtcttcggc cagccgagaa ggtcggcctt ggagcggttc cactgggcgt
    11461 aggccaggcc gacccggccg aggagcttct tgtcccggat cagccggtcg atggcgtgaa
    11521 gatagcgcgg gttggaccgg cgctggtggc cgatttggag gagacgcccc gtccgccggg
    11581 ccgtttcgac cgtcgtccgg gctttctcga gggaattgga catctccttt tcgcagtaga
    11641 catgaagacc gttttccagg caggcgttgg tttgttcggc atgaagccag tcgggcgtgg
    11701 ctacgatcgc cgcggccagg cccttctcct tggccagcag gtcgcggtag tcctcgtaga
    11761 ccgccggatc ctgaccatac ttgcggaggt agccccgggc gtattgctgg ctgtaggacc
    11821 agatgtcgca gaccgcggcg atgcggatcc cggggatccg gaggcacgat tccatgagga
    11881 cgcggccctg ttcccccgcg ccgatgagag cgacgggaag atcgccgggc cgccccgggg
    11941 attcagcctg ggaggaggcc gggtccggag acggccgggc gagaagggcg cccccgacgg
    12001 ccgcgcccag tccggcggcc gccgaagttt tcaaaaagga ccgtcgcgaa acggcgggat
    12061 cgtttttctt ggacccaaaa tcggtcatat ccatccgcct gtcgagaatt ccgaacgccc
    12121 cctattatat tcatttggag gtttctgaaa acccttcggc cgcggaaaat cgcgtcttgg
    12181 gggaatcgga tgtcgggatc ggaacgccgt ccccccttcc ggcggcggtg ccgggagatg
    12241 ttccgggcgg cctacttttt ctcgacgggg atgaggattt cggtctcgag cttttcgggc
    12301 gggacttgtc cgggatcatt cagatagaat tccaccggcg gccccgcggc tttccatccg
    12361 ttccgttcga gataagcccc gattttttgg taggcttcgc cgaccttgtc gtagggaccg
    12421 acgtgaaggc acgaggcgac cttgggataa gcgaattcgt ctttccggag gggctcttgg
    12481 ggcgtgacgt ccgcgccgat cggcatgccg atccgccact tcagctcggc ctccggaacc
    12541 tcgccggggg cgttgaggta catggccatc accccggtga aggatcccaa gccttggctg
    12601 aaatattcat tcatgaactc gccgattttc gcggaaatca gcgaatacga gccggtgcat
    12661 tcgatgtagg cgtaaacgaa cggcgtttgg tccttgacga cgacgtcctg tccgagaccg
    12721 aagccggcgc cggccgcagc cagaaggatg aaaaggacgg ttttcttcat ggtgtctccc
    12781 ttattcccgt ttttccagcc cggctggacg gacctgaaga aaaaatcata accaaaggcg
    12841 gccgcgaatg caatcagcgc cgggaggggg cggacaatat gataaaatac cggcacaccc
    12901 cgccggatga caagcgggat ggaaagggcg aagcatgaaa aaaacagcga tagcggttct
    12961 cggggccgcg gtcttgatcg gcgcgtctgt ccgcgcgtcc gagcccgtcc ccggtccggc
    13021 cgggaatgaa gccggatcga cggcctttat caacgtccgg attttcgacg gagaaaaagt
    13081 catccccgcg gcgacggtcg tcgtcgaggg ggggaggatc accgccgtcg gaacggaggc
    13141 ggccgttccc gccggggccg agatcgtcga cggggcgggg cggacgctgc ttcccgggct
    13201 catcgattcc cacgtccata tctgggatga aagcggactc cgggaggcag ccgtcttcgg
    13261 ggtgaccgtc ctcgtagaca tgtttacttc agccgagttc gtctcggctc aaaagaaggc
    13321 tcaggcggcg ggattccggc ccatggctca cctcgtctcg agcgggattt tagcgaccgc
    13381 gcccggaggg catgggacgg agtacggcgt caaggtcccg gctttgaccg ctcccggcga
    13441 ggcccaggcc tgggtcgacg cccggctcgc cgagggctcg gatttcatca agatcatccg
    13501 cgacgacgga tcgacctacg gccgcgcgat cccggcgatt gcgcgggaga cggaagccgc
    13561 cctcatcgcc gcggcccggg ccaggggcag gcggacggtc gtccacgccg cgaccttgcg
    13621 gcagtgccgg gaagccctgg aggatggagc cgacggattg gcccatcttc attttgacga
    13681 cgcgttcgac ccggatttcg gacggctcgc ggcctcgcgc aaggccttca tcattcccac
    13741 cttgaccgtc ctggccgcga tgaacggctc tcccgacccg gccggcgtcg cctccgacgc
    13801 cggcctgtcc ccttatcttc ggcccgagga cctccagatg ctcggccaga cgatgccctt
    13861 ccggtccgcc ccgggcgctt atgccgcggc cgaacggacg ctccgcgagc tccggacggc
    13921 cggcgtgccg attttggccg ggaccgatac ctccaatccg gggacggctt tcggagcggc
    13981 tcttcacggc gagctcgctc tcctggtcaa ggcgggcttg acgcctctcg aagcccttcg
    14041 aagcgccacc tcaatcccgg ccgctttatt cgacctcggg gggagaggcc ggatcgcgcc
    14101 ggggagccgg gccgaccttc tcctggtcga gggtgatccg accgttgaaa tcaaggcgac
    14161 ccgggccatc gtcggtgttt ggaaggacgg acttcggatc gatcgggagt cctggcggag
    14221 cgcggccgcg gaagccaggc gggcgaggga aaaccagaaa aacgccccgg ccccggaagg
    14281 gctgggggac ggcctgatca gcgatttcga ggacggcgcc gtttccgccc ggttcgggtc
    14341 cggttggacg gcgtccacgg attccctcat gggcggcaag tcccgggccg agctttcgac
    14401 cgtggatgga ggagccgagg gcagcgcccg ggccctgaaa atcgagggag agatctccga
    14461 gacggggtcg atccgctggg cttcggctct cttctcgccg ggccgggcgc cgttcactcc
    14521 ggccaatctt tcctcgaaaa aagccctgag cttttgggcc aggggcgagg ggaagacgtt
    14581 tgccgtcatg atttatgccc agagctcggg cttcatcccg aagatcgcct tctttccggc
    14641 cggtccggaa tggaaggaag tcgtccttcc gttcgagaag atcgggctcg agggctttga
    14701 catcctgggg atcgccatcg gggcgacgac ggcgcccggc ccgttttggc tcgagatcga
    14761 cgacgtccgg ctgaaataag cgccggcgat ttcgtcggct ttcgggcgtt tcccctctcc
    14821 gcccggcctt ccggactcag cggaattctg cgtcgatgat cgtgatccgg ccgggataga
    14881 cgatcgggcc caggggagtg gtgaccgtgg gctgggcgtc gagggcgacg cgggcgggat
    14941 cgcgttcagc tccgcccaag gccagggcga ggttgaggag cccctcgtaa cccttggccg
    15001 cgaagaattc gtagagatcg atcgtcatcc ggatggggat gacggtgttt cggcccgtcc
    15061 cggggatctc aatcgcctgg tcgatattgc cgatgaccgt cggcgtgccg tcgatgagga
    15121 ggcggctttc caagccggtc agcgtggaga cggtccgggg cgatccgccc gtgccgtcgt
    15181 tgggattgcg gacctcgacg cggaggacga attccgccgg caggcgtttg gccgtgaatc
    15241 cctggacgag cttgactccg tccatgacgc tgaaatcgtt cagggcggat ttgccctcga
    15301 tccggatgcc caggaccgaa aaatcggaga cgtcggccag ccggaattga agccgctgga
    15361 gattcgaaag ggcggagagg aacggctcca gggcggcgca gcttgaaagg agccccgcgg
    15421 ccaggatgac gagggccgcg aggattgcgg ttttgagacg tgaacgcatg ggcggctctt
    15481 ccttaaccgg cgtttttatt ttttgttctc cgcgccgctt ttcacccggg cggcgaaaat
    15541 ctcgtagaga aaattcggca tcagcttgag gacccaggcg ctgacccgca tgaggaaggg
    15601 gaaccggatg tacgtctttt cttttttcag gcccttgacg atgacggccg cggccttctc
    15661 ggctgaaacg atgaacggca tcttgaagtc gttgccgtcg gtcatgggcg tcttgacgaa
    15721 ccccggcttg acggtgacga ccttgatccc ccgaggcttg agctcgatcc ggagagcatc
    15781 caggatcaac gacaccgcgg acttgctggc gcagtaaaat ccgttgcggg ggaccgcccg
    15841 gctgtcggac aggctggaga cgccgacgat ggtccccgat ccccgctttt tgaaatcggg
    15901 cagaagctcg ccgaggaaat cgacgactcc gaacacgttg acgtcgaaca tagtccgggc
    15961 ggctccgacg tcgaagagat tcataccgcc ccggccggac accccggcgt tgaggatggc
    16021 gatgtcgacg cccccgaacc gggatcggac ttcccggtaa gcggcctgga tctcctcccg
    16081 cttggagacg tcgcacttga cgggaaggac ggcgctcccc gagtgctgaa gctcagccgc
    16141 cgtccggcga aggacgtcgt cccgccgggc cagcaaggcc aggttgacat tctcctcggc
    16201 caggagccgg ctgagggcga gacccaggcc gctggaggcg cccgtcacca ggatcgtttt
    16261 tccctggagg ttcaaggcca tctcagcggc tcccctccac gaggccctcg aggacgctct
    16321 tgtgaaccat gaaatccatg atcttcagct tgaggtactc ctcggtcaga aagtaatagc
    16381 ccttgggatc gaagttgaag gcgctcgatc cggagtcctt ggccaggaac cagagccgcc
    16441 ccccgtcctc cttgtatccg atgaggtgga tgccgtggtc gtcggtcgtc gtcccgttgc
    16501 cgaagcggta ctgccgggcg ttgtcgtcga tgaattccgc ggggatgtcg aacgtcggga
    16561 tcatgtggac ttttttctcc gcgttcttgc ccggctcgga gatgtccccg ccgatgctca
    16621 tcgtgaagcc ggcggccagg cccttcttga ggatcttcat gaaggtgtcg agggggacgt
    16681 tcacgtaatc cttgctgtgc caccagttgt ccggcacttc gtactcgacg ttcgggccga
    16741 agggctgctg caggtaggac acaacgtcga cgtaatcgtc gggattgatc ttcaggcagt
    16801 cccgcaggta ggtccggggc gtgtattccc gtccctcgac cgtgaacttc tccggcgggg
    16861 tgcccatgtg ggcgccgaga atggcccgga ccgtctcgag ggcggcggcc tcgttccagg
    16921 cgccggcggt cttgacatgg tcgaggtagg ccttgacttc ctcgatgagc ttggaatgat
    16981 catggaattt ttggcccggc ttcagcccgg agaaggcctc ccagggcatg cagccgtaaa
    17041 tcttccaatt ccgggtgacg gcgttgccct cggatccctc ggccacgtag gaatcccccc
    17101 gctcgcggat gaaccgcttc gtccgctcca ggtattcggc gtagaccgtg aacatctccg
    17161 agagccggac tttctttccg tggatccggt agatctcgga ttcgaagaag gaggtcgtgg
    17221 cgaagctcca gcaggtgttc gtgttcccct gggagaccgg cggattatgc caagccgtct
    17281 tgaagccgtc caagcttttc gggacggcga atccggccag gtcgaccatg aacgtccggc
    17341 ggggaggggc cggccgggcc tttccgaatt ctccggtcgt cctggcgatt tcctcccaga
    17401 agggattctt gggctcgatg aaaatccctt tatctttcgg cttgtcctga gcggcggcgg
    17461 aagcggcgag aagcagcgac gcggccagca ggccgaggat ggtttttttt ctgaacatgc
    17521 ggcacctcgt tgcgggtgga attaaaaaag gcatcggagg aaaaagatcg tatcgccggc
    17581 gggggattgt gtcaaggaat cgccggcaag gcctcgggcg ccgattcgag gcccttgagt
    17641 tcagcggctg gggatcgttc ttcgttttgg ggagagccgg ctcgctcggc gcgtcctcga
    17701 tgcggccgga tgagccgccg acggcgcgct ccgctcccgc tccggctgtt tgatcacgga
    17761 ggtgttcggt ctgcggcctc agcctcgagc ctgggttttc ggttcccggg tctcaggatt
    17821 cgggcggcgc gtcggctagc tggaaaacgt cgtcgacctt cactccgaaa gcccgggcga
    17881 ttttcagggc caggacggtg gaggggacga aaacggcgtt ttcgatggta ttgatcgttt
    17941 tccgggtgac gccgacccgg cgggccagct cctcctgggt ccagtccttg atcgcccgct
    18001 ggacgcggat cgtgttgcgg agctcctcgc cgcccacgtc agcgcctttc catgaggacg
    18061 aaggacaagt ggtatgctcc gaagccggcc cagagggccg tgaagatgac ggcgttcgtg
    18121 tccttgaggt cggcgaagac ggaaatgacg ccgaggacgg cgaggacgga ggccatggcg
    18181 atgaatccgt acatccaggc ggccttgccg tgggcttgaa tcctctcatc cgtgaacagg
    18241 acgcccagct ccgcgtcggc ttggccgcgc ttgcgaagcg cgctcaagcg gacgacgctg
    18301 taaaactggg cggggagggt caggatgagg atcaccgcga aaccgatgtc cagccattcg
    18361 gggagcgtgc cgatggtccg gacgatgaaa cggaccagcc aagccgcgga gaaaacgaga
    18421 aaaacgatga cgaaccggga atagacgccg cggtacgtcc gctcgaattc gtcgattttg
    18481 cttgccgggg tcatgggaaa tctccttttc atgttcctgt ttcctcggag ggactcaacc
    18541 gatcacaagc atcgaaagaa agatcacgac gaccactaaa accaacattt tgaaattgct
    18601 cattgttaac gcctatgact caaatagtat cataaacgtt atatataata acgcaaaggt
    18661 tacgcttgtc aagagaaatt taatattttt ttttagttcc ctgtatttcc tttaaatcct
    18721 tttctttata ggaatcggaa aatcgtgcgg attcagctta atgatcgcga aaacgacgcc
    18781 cgtcgtcagg actgaaaatc cttcgccccc tggccggaaa gaacgaggcg ggccggtctt
    18841 gccccgagcc gcggcgcgga tcgtcagcgg attccggccg gagcgagcag ggcggcggcc
    18901 aattccgcct gccgggcgaa atcgatcgcc tcggccagga cgcgggcggc ctcttggggg
    18961 ctgtgaaaac ccgttccgtt ctcggaaaag acgaagtccc agcgaagctg gccgcgccgg
    19021 atcagagccc gggaagcgga caagcgctcg tccggaacgc cggccgcctt ggccttgatg
    19081 atcgcgtcca tggccgcaag caaggccgtt tccgtcttcc gcagaaggcc cgccgtcttg
    19141 tcctggatgt ccttgacccg ggattttaat tcctcctcgg gccggggatg gcaggcttgg
    19201 caggcggcgg cgatgttcgt cagggggctg cggatccagt ggtcggaaac cttcacgctt
    19261 ccggcccgga catacggcat gtggcagtcg gcgcaggcca ccccggactt ggcgtggagc
    19321 ccggtgcccc acgtttcgaa ctcgggatgc tggaccttga gcatggccgc cccggtttcc
    19381 gcgttgaccc agtcgacgtg gccgttttca tcgtagcagg cttcgatcgc ctcgatcgtc
    19441 cggcccttgg cccaggggaa agtcagttcc ttgttctccc cgtaaaagta gtattcgacg
    19501 tggcactggg cgcagacgta ggtccgcatg tcctggcggg tcgccttggc caagtcgacg
    19561 ccggccgcgg ccatggcttt cttgaaagcc gggcgggtga tgaccagctc cattgtcttg
    19621 gggtcgtggc agtcggcgca gaccgagccg tggcggagtt tgggggagag ctctttgtac
    19681 ggcgttttat tgaatgcctc ccagcccatc tcccggatca gggccggagc cttgcccgag
    19741 tggcagttgg cgcaggctcc gggctgatcg aattcccgga cccggcgggt catcttctgg
    19801 tcggtcaggg cgaagaagtg accccgctcc tcattgtact cgacgctgaa ggcatagccc
    19861 gcccagagcc gcttggccgc ggggatctcg tcgatccggc tgaagcgctc cgatccgccg
    19921 tggaacgtcc ggccgtaatt ctcccgcgtc cggacgaacg tatcgtactc gaggggaaag
    19981 tttttccccc agacggccgg atcgatctcg ccgtccttga tttcgacgac cttcaaggga
    20041 tagcggtacg cctcctcccg ccgggttcgg acgttgaaga gaagggcgag gacgccggcg
    20101 atgaccgcgg ccgttgccag gaaagccgcc ccgtacaaaa gcgcgttctt ccgcggacgc
    20161 ttcgtttcca tcatccgatc tccttcgtcg tcagtgtccg gcgttccggt ggcagcggct
    20221 gcagtcggct tcctcgcccc ggctgttcgt ccgcatccgc tcgatgaccg gctcgtggca
    20281 gcgccggcag ttgtccagca ggaccctctt gttcaacggc cggatgcgga tcggctcgtg
    20341 gaagtcgccg gtcgtgaacg ccttcccgtg attaaagccg ttgattccct tgaccgtcca
    20401 gtggccgagg aagccgggcg gcgtatggca gtcgttgcag acggcgacgg ccttgtggga
    20461 ggcccggttc cagtcgtcga aatggctccg catgatgtgg cagttcatgc agattttcgg
    20521 atcgtccttg aggtaggacg agccctgggc gaaaaagaaa gtgaatccgc ccagcccggc
    20581 ggccgccccg gccagggcga aaagaaggac ggccggtccg cgccgtcggc ggcgggtcgg
    20641 ttcggtcgtt tgttccggcc cgttcatcta acgcttattc tagccggaac gccggcgttt
    20701 tcaaagcatc tcccaattca agagccgccg ctcgccctgg agggcccgga gcggggcgat
    20761 gtcctgggac acctcgatgg ttccgcgata ggagccggcc tcgtcgcgga cggcgaaata
    20821 gcggatgtgg atgaacggcc cgccttcccg ctggatccag aattcggcga cgtccttcgt
    20881 cccctcctta aacgcttgga cgatctcgtt gacgatgtgg acgctcttgg gcggatggca
    20941 gttctggacc ttgcggccga tgacttcggg gctgcgggga aaaatccgtt ccggcccgtc
    21001 cgagtagtaa gcgacgcggt cgttttcgtc gacgaaggtg atgtccacgg ggagccggcg
    21061 gagcatcaga tcgatctgct cgggcgtcag gcgcccctgg gacaagcgga tggtttcgag
    21121 ggccggaccg atcccggagg cggccggacc ttcgccgccg gcggataatt tctccgctcc
    21181 gcgggaaggc gcggccgggg cggacggcgc cggctcggcc gccggggcgc tcatcgattc
    21241 ggccaggcgg gcatcccaca cggccgaggg cttgatccaa gcatagccga tcgccggttc
    21301 gccgtttttg atccggaccc agtcggatgt cttgagcttg cgagccgagg tcgggaatag
    21361 aatccgttcc tcgagaaaca tcattttctt gaccgcggag gcgagcgttc cgaaccgctc
    21421 tttcaagccg gcccaatcgc cggcggccaa gaggtcggcg gccgacttga gg
//
LOCUS       DAOOXH010000035        21122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_2349_length_21122_cov_8.353681, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000035 DAOOXH010000000
VERSION     DAOOXH010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 21122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21122
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2349_length_21122_cov_8.353681"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PKV64_05750"
     CDS             complement(<1..234)
                     /locus_tag="PKV64_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16620.1"
                     /translation="MKRLIVVFLTAAMIPALVLGTRLERIDHWKHEQTYWGPISGSFL
                     DSAGDLILMFRKSGPVAVSHDKTATGPLFLNIKI"
     gene            complement(816..1622)
                     /locus_tag="PKV64_05755"
     CDS             complement(816..1622)
                     /locus_tag="PKV64_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPN16621.1"
                     /translation="MYKPAKKDLVSLKGEKRDFKMKRGKLIIAGNNIKSPSAISLLSI
                     IKKLNIDGSRWELIINPTIAAIARIRNNDAIIMACDYNCSADYCGRWFFCLGNKPIVR
                     FAIFRRLPPNLEKKSIFYSFRVLRGFDSTNPDEYFSLIKNYPGCVGLEDGKYSSHVPL
                     NKIAKIEQIINKYEGADFDREYLSLKIKLSSSYISFLFKERMGITLRTYTQKMRFCAA
                     LRKVLSTDKQVKQIAYEAGYADPLYFGKAFKQWIGMSPKNVRRRYSSNII"
     gene            1928..3001
                     /locus_tag="PKV64_05760"
     CDS             1928..3001
                     /locus_tag="PKV64_05760"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HPN16622.1"
                     /translation="MKKTRAAIILAAVYYLSPGGCSRNPSIPMEIIEITPLLELNAVI
                     PDYKIMPFFNLFQNMAIDQQGNIYYLKFGHDRIQKFDKTGRLAADIGGIGQGADALFS
                     PTAILVENDSLYVLNDAGRQVKIFNLDGEFKSSFKIDNAWPPTSLCVDREAIYVNVID
                     KSNKTRNDGKLISVYDHEGRLVKKIGPIIKCNSMIGYNTFNRIRMIARDENIYYAFIN
                     QPLISWSGKNGGRTAVLDLRKFGIPVIDAIAGRIDAEGIDTPETFKDDSVSSGTCISF
                     CSGFAVDEAGRIFYIPNTAGSGSRDEWDNSILLFNENHKLSKKIYLKTGKETVTPRDF
                     LIAGGKAIYGIGTIKERSILFKF"
     gene            3038..3646
                     /locus_tag="PKV64_05765"
     CDS             3038..3646
                     /locus_tag="PKV64_05765"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HPN16623.1"
                     /translation="MGTRRNAIAWIFSLFLWLTASGAAMIAHEACKIAENRAAHGTLA
                     LELEEEAVIRESNENDDYRFSFLRDLAADAAENLFVLDKNSILKYDRRGIFLKSFGRK
                     GQGPGELMEPRSLFVDEHDDLFVNDQGGLLHVFDKEGTFRHQIKLDFQIPFDSNAFYV
                     TGEGFIYAFSWDMINLEMKKTFVKADSSGAVILRLKTMDDAG"
     gene            3910..4149
                     /locus_tag="PKV64_05770"
     CDS             3910..4149
                     /locus_tag="PKV64_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16624.1"
                     /translation="MSDDKGRIYAIRVKSVWDENKAEKADIFSRHGRYLYRTEFPATP
                     SLIKNDSVYFIDEGQDGLKVIKRVKIRNYLQMKEE"
     gene            4313..5098
                     /locus_tag="PKV64_05775"
     CDS             4313..5098
                     /locus_tag="PKV64_05775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN16625.1"
                     /translation="MEIIDLDERFENDYFVCLEGWSDEPKEAGHHRETWFRRMKEKGL
                     RVKIALVDGRPAGMIQYVPIEHAFAEGRDLYFIHCIWVHAYKGKGIGDQRKKGLGSAL
                     LAAAEEDARAKEAKGMVAWGVGLPVFMRASWFKKHGYRKVDKNEVALLLWKPFTPDAV
                     APRWIREKKRPAAVPGRTSVAVFRNGWCTAMNMVGERARRAAAEFGDRVAFTEVDTSD
                     RAAYLEWGIADGLFIDGKPVRTGPPPSYDKIRRKIARRVKKIK"
     gene            5095..5565
                     /locus_tag="PKV64_05780"
     CDS             5095..5565
                     /locus_tag="PKV64_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HPN16626.1"
                     /translation="MTSMTPAVRVLKDLAADFEIRLYRYEEHGGTAAAARVLAVEEHD
                     VIKTLVFETDGRTPFFVLMHGDREVSAKRLARILGVKSVAPCPPAAAERHTGYQVGGI
                     SPFGARKNLPVYMERTLTESPRVWINAGKRGQLAGMAPAEILRILAPVLIEAAV"
     gene            5693..6400
                     /locus_tag="PKV64_05785"
     CDS             5693..6400
                     /locus_tag="PKV64_05785"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HPN16627.1"
                     /translation="MLQEIQKLNERFAAAEAEDILGWMVRALPYQTAMTSSFQASGVV
                     LIHMLKKIAFDFPIYFIDTGYHFPETLEFKARLTREWKLNVHTIVPEMSKVEQLSLHG
                     PRLFERDPDLCCRLNKVDPLRGLQRELGLKAWVSALRRDQAETRRNLRPVMIDESGGL
                     RIHPLLAWNRERVWAYIEKHRLPTNPLYEQGYASIGCFPPCCTSKSEPGSDERSGRWA
                     GKGKLECGLHSHLKSEK"
     gene            6415..7779
                     /locus_tag="PKV64_05790"
     CDS             6415..7779
                     /locus_tag="PKV64_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPN16628.1"
                     /translation="MRKKFVLRCVLCGAEIDGFGPWFEAGQRCPECGSDRADVVYRTT
                     PAKVRDLFRRPANGLPGMWRYFDVLPVNDRKNIVTAGEGLVPIDRWEFLEEYARRRFQ
                     IRCQVYAHRQDDNYATGTFKDLSGSIVSSVLKENGIQNFVASSTGNTAVAYARYLTAA
                     GISFYAFIPKNASLTQAAEIGCFGQNVFRVKGDYTLTKEIAMAFARKHKMPLAGGNFD
                     PMRIEAKKTMMLEWIRLLGKFPTVYIQALSGGTGPMAIAKGCRDLAPVGLGGRMPRFI
                     LSQSSRCAPMAAAWAEAKKKKYPEGWEKTYPIYHNPRTGIATLAAGFPKTYPVLAPLV
                     RESGGEILSFDEDATPLIARLVAFERSVRIGPAAAIGLGGFFEALKRGLLRNGDRVLI
                     NIGEGIRRAPEFLITMFQEKAEVATVDACRLFDRRGHRADIWNELEKRVMNGKTAPAS
                     RRGR"
     gene            complement(8166..8702)
                     /locus_tag="PKV64_05795"
     CDS             complement(8166..8702)
                     /locus_tag="PKV64_05795"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HPN16629.1"
                     /translation="MTPEQLKQRILEVKDFPKPGVGFKDIMPLVLDPESYHYTIDRLS
                     AWARNLKPDVIVGIESRGYLFAAPVARDLRLGLSVIRKKGKLPRKTVCVQAPNEYAVE
                     FFEKHADALAAGQRVVVIDDVIATGSSSISALRLVKMLKAKPVGFGAVIELAFLGGIP
                     AIRKAHPDVDLFHLVRYE"
     gene            complement(8798..9292)
                     /gene="tsaE"
                     /locus_tag="PKV64_05800"
     CDS             complement(8798..9292)
                     /gene="tsaE"
                     /locus_tag="PKV64_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPN16630.1"
                     /translation="MTKPAPAEDQVLTTRTERGTYLAARRLAAAFRGDEVVFLTGELG
                     AGKTVFARGIAAGLGMKNPRLVCSPSYTLINIYQGRVPILHLDLYRLGGQAEIRDLGF
                     EDHIGEAVILIEWGERIDFPLDGIRVHIGVEPDGTRTIRVKRPACGRNRAVDRSRFSA
                     RGDR"
     gene            complement(9289..10881)
                     /locus_tag="PKV64_05805"
     CDS             complement(9289..10881)
                     /locus_tag="PKV64_05805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPN16631.1"
                     /translation="MKILTAGQMREIDRRAFEEFGIPGVLLMENAGRRVAEEVRSLRG
                     NLAEERIVIVCGRGNNGGDGFVAARHLFNLGARPRIFLLAGKNEVKGDAALNLDIVLR
                     LGLEIVEVTAAAGRSRLNLKRALREATVIIDAIFGTGLSKPAAGLAAAVIGDINESPA
                     ETIAVDLPSGLSSDTFAVIGPAVRADATVALGAPKIAHVFPPAEDYAGRLVVAEISLP
                     PVLLASPELKLEMAGPENAVPFFRPRPRSGHKGTFGHVLILAGSRGKTGAAVLAGKAA
                     YRSGAGLVTIATPESCVPAVARTMSELMTEPLPETEEGTIAGAARPRLERLLSGKDAL
                     VVGPGLSTHPETAALVRSLLPGVRIPTVIDADGLNILVGKLGFLKKTARPPVLTPHPG
                     EFGRLLGRSVPEVLNDRLALASRFAAEHRVLLVLKGYRTLVAGPDGRVSVNPTGNPGM
                     ATGGSGDVLSGLLGAFLAAEKNAFGAAVASVYLHGAAGDIAAAELGQRAMVAGDILKA
                     LSRAMKALEDAAGGRKGRLFQP"
     gene            11038..12036
                     /gene="gap"
                     /locus_tag="PKV64_05810"
     CDS             11038..12036
                     /gene="gap"
                     /locus_tag="PKV64_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPN16632.1"
                     /translation="MPIKVGINGFGRIGRNMLRASGGSGIEFVAVNDITDARTLAHLL
                     KYDSVLGVFPGDVQSTSDTIIVDGHPIKVLAAKDPGELPWKDLGAEIVVESTGKFTKR
                     PDVVKHIEKGGARKVIVSAPATDPDVTIVMGVNERIYDPTKHHILSNASCTTNCLAPV
                     VKVLHELAGVEKGFMTTIHSYTNDQKILDAPHKDLRRARAAAVAQIPTTTGAAKAVGI
                     VLPELKGKVDGIAIRTPTPDVSLVDFVAVVQKPAPAEVVNEAFKTAAAGPLKGILDYT
                     DEPLVSVDFLHNPHSAIVDGLSTKVIDGHLVKILAWYDNEWGYSCRLIDLVKYIAR"
     gene            12044..13216
                     /locus_tag="PKV64_05815"
     CDS             12044..13216
                     /locus_tag="PKV64_05815"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPN16633.1"
                     /translation="MKLIEDLDVRGKTVFVRVDFNVPLSESGEIRNDTRIRASLPTIR
                     FLSEHGAKLILASHLGRPKKFDPAASLRPAAVRLAELLDRKVEMAPDVIGPDVDRLKA
                     GLRDGGVLLLENVRFQPGETKNDPELAREFARGVDVFVNDAFGSSHRAHASVVGVAGL
                     VPAKAAGYLLKKEVEFLGKVLHPVKPFTAVLGGAKTEDKIPVIESLLDKADDVLIGGA
                     MAYTFLAAQGKGVGKSLVEPDKFDLALGIMKKAADRGVRLRLPSDHALAPSIDSGVID
                     RMTGEYPFPDVLLGVDIGPKTIATYSAIIGRAKTVFWNGPLGVFENPAFSEGTMQIAQ
                     AVAASPAVSIIGGGDSIAAVAKAGVGDRIGHISTGGGASLEFIAKGTLPGLDALEG"
     gene            13227..14003
                     /gene="tpiA"
                     /locus_tag="PKV64_05820"
     CDS             13227..14003
                     /gene="tpiA"
                     /locus_tag="PKV64_05820"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPN16634.1"
                     /translation="MKKRPFVAANWKMNMTAGETRAWFEAWASLAGTEEADVVVCPPY
                     TALAEAARRAAGIAVHLGGQSLHWEDAGAYTGEISGPMLADAGCRYVIIGHSERRRYF
                     GETDETVRKRIAAAVRSGLRPIVCIGETLAERDEGRTLDVIRLQLDGGLAGLTEETIG
                     RLIFAYEPVWAIGTGRTATPEQAQEVHRAVRGRLAEKIGKAASGYVIILYGGSVKPAN
                     ARELLRKPDIDGFLVGGASLEAASFGEIVKESIRAYKEVN"
     gene            14005..14352
                     /gene="secG"
                     /locus_tag="PKV64_05825"
     CDS             14005..14352
                     /gene="secG"
                     /locus_tag="PKV64_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPN16635.1"
                     /translation="MTALLTIIHVIVCLVLIVAILLQSGKAADLAGAFGGGGSQTAFG
                     PRSTQSLMSKITTICAVVFMLTSLGLWIFSGREAPSAVKGVTAPAKTEAPAAAPAEKT
                     PGQQTPATEVPKQ"
     gene            14368..14455
                     /locus_tag="PKV64_05830"
     tRNA            14368..14455
                     /locus_tag="PKV64_05830"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14402..14404,aa:Leu,seq:gag)
     gene            14841..15575
                     /locus_tag="PKV64_05835"
     CDS             14841..15575
                     /locus_tag="PKV64_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16636.1"
                     /translation="MPLLAAVPEPEEIIAFPAGATSGLKPEKEEKTGNAGGIFGFYYG
                     MSDTFSLSFLYNISGDNKVFGLPEQFGSFLLNPSSNGSSFLATGEYILKNGAKTSLGL
                     SFRGGISFTDWEATIAGAAVSHKGQVFFFVPSLRFCTNERNFGDNNTLKIIVDVGLGI
                     RGIIGNLGQTPEFLSDKAVLGWDKKIFVGGELTISARINKITPFIRITCYSPKSDIPS
                     FSDLQAYIGIDVISTLFSSKVNSPEE"
     assembly_gap    15769..15868
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15869..16483
                     /locus_tag="PKV64_05840"
     CDS             <15869..16483
                     /locus_tag="PKV64_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16637.1"
                     /translation="INSFSASPETIDLGSASTLSWSVSNAQNVVIDQGIGTVDSTGSI
                     AVSPNATVTYTLTASNANGAVSKSCLVTVNQPPVINGFAATPNVITLESSSTLSWDVS
                     NAVTRTIDPGIGQVSATGSTIVSPNSTTVYTLTAVNGSFTVTATCMITVDWQNFICYK
                     TATGTKYHRGNCQYLSQSKIQTTLGEACAQGLTPCSVCNPPSCR"
     gene            16517..16723
                     /locus_tag="PKV64_05845"
     CDS             16517..16723
                     /locus_tag="PKV64_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16638.1"
                     /translation="MTGKEMKSILIILACAFLSAASIQKQSSRFCIKVIDGDTILLDG
                     DETVRLIGVDCLETKDARHRTQPG"
     gene            16695..17720
                     /locus_tag="PKV64_05850"
     CDS             16695..17720
                     /locus_tag="PKV64_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16639.1"
                     /translation="MRVIGHNLDERTRFPGAVFINRKGVTKVSVSYAALMTGRPASGP
                     PLTWTSKYDSLTFNAFGRDFPDGGLNEAGLFVGEMSYPPSKFPADPDKPAMFVNLWIQ
                     HLLDTCDSVDQVEASASALNLDGWGWHFLAADRSGGAAAIEFLDGRVVVHRGDSMPVP
                     VLCNEPYEYEMKRREAYFAARESRTGSAAEEIPRFIQAGEMIETYGRRSSGERKEAVD
                     YAFDILAALERGGTQWSFVCDLQNGEAWFKTAATPKVKHVALGGFGGGDPASARFLNM
                     DADFSGEAGSRFEVYSPAANREIAERAFEAILRISPEFEKVIERIGETRASLVGRFAG
                     YPETTSR"
     gene            complement(17740..18177)
                     /locus_tag="PKV64_05855"
     CDS             complement(17740..18177)
                     /locus_tag="PKV64_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPN16640.1"
                     /translation="MNEKIKISCLGCGGTNQIPEEAVGKTAVCGRCKTPLPVPGEVLE
                     PTEEQLAVLIQKAALPVLIDFYSETCAPCRVMHPVVEALAKRRAGNLMVVRVNTAVNP
                     QAAGAFQIQAVPTFIVMRRGHEAGRTMGAMPETDFSLWVASLS"
     gene            complement(18174..18956)
                     /locus_tag="PKV64_05860"
     CDS             complement(18174..18956)
                     /locus_tag="PKV64_05860"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPN16641.1"
                     /translation="MTNRLKEDKKSGAAALRPENAARASIASLKACADPKRAEGQRRY
                     FKEQVEFLGLTTPKMREIEAEVWQKVQGTWGFPEAVAFAETMLKERFYEIRALGLLIL
                     LRFRKEFPASFPERVRGWLRADRLDNWALVDVFCPDALGPILEKDPKFVTEIKTWTAD
                     SNRWVKRASVVSFIKIARRGTHLDAVYEIAVRLFPVQDDLIHKATGWLLREAGKADAP
                     RLRAFLLKHGPAIPRTSLRYAIERFPETERKALLAKTKMVKP"
     gene            complement(19065..20378)
                     /locus_tag="PKV64_05865"
     CDS             complement(19065..20378)
                     /locus_tag="PKV64_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HPN16642.1"
                     /translation="MSKTISPERFAARNAAFLPEHFYNIKADLPACPPPPLHPGTKAP
                     LRPSDLEAVFSRGFIAQEITRERFIPIPKEVREKLAVYRPTPLIYASGLRRALDTPAH
                     VFYKYEGVGPVGSHKSNTALMQAYLAAAEGVETLVTETGAGQWGSALAQAGSLFGVDV
                     RVFMVRSSFRQKPGRRTVMEMFGAKVEESPGPSTNFGRALYEKDPDHPGSLGIAISEA
                     IEVAVGGKNSKYALGSVLDAVLMHQTVIGLEAAAQLSAAGAEPDVVIGCVGGGSNFGG
                     LAFPFVRGSLAGERKTRYVAVEPEACPSLTRGDLRYDNGDTAGLTPLLFMHTLGRDFV
                     PAPIHAGGLRYHGMAPLVSHLVGQGMVESRSVPQAEAFDAGTLFFKTEGILPAPESAH
                     AVAAAITEAAEARASGQERVILFNLSGHGFLDLAAYAKNGRNSSS"
     gene            complement(20782..>21122)
                     /locus_tag="PKV64_05870"
     CDS             complement(20782..>21122)
                     /locus_tag="PKV64_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16643.1"
                     /translation="TKGFFTMSRSSRDIVKNPFVLKKEFADLKATLHRADVGARERDK
                     AETRLAELENKLINRSVSVYGAVNLRTDGRNLLMCQKKEAPNRQREEWDIYLLEPDAT
                     TGGLRFIPRN"
BASE COUNT         4429 a         6294 c         6126 g         4173 t
ORIGIN      
        1 tattttgatg ttcagaaaaa gcggaccggt ggccgttttg tcatgactca cggccaccgg
       61 tccgcttttt ctgaacatca aaataagatc ccccgcggaa tccaaaaatg accccgatat
      121 cggtccccaa tacgtttgct cgtgtttcca atggtcaatc ctctccaggc gcgtccccaa
      181 gacgagtgca gggatcatgg ccgctgttaa gaagacgacg atcaatcgtt tcatcggccg
      241 ccctccttcg aggaaccgca taaggagccc ccctcccgca ccggatctta gagatatatc
      301 atcaatcggg atccagttgg ataaggcatt tacagtttat aaaattattt tctgtacagt
      361 cacagtccca ttgattgggc cctgttattg ttttttttcc gccgagcaaa acagttcccg
      421 cttccgtggc cagaggcacg attaataaaa acgctagaga aataattaaa ccgcagagaa
      481 caagaaattt aaagtttctc atgacagtat ttctactttg gatcgggcgc atagatacat
      541 ctacaatcag atacgattac aggtgcagga caacagcata ttaagcctat atgtattcct
      601 ggcttaataa ttggatcgac tgcttgtagt ttttgaggta caaggaaaat ggcgatacta
      661 gctgccagca aaacaactaa cagaactgtt tttgctttct tcatcttttc ctccttttgt
      721 attttcgacg gttgttttgg aaagaggatt ccttttttct aataaattaa caagagaaga
      781 tttgtaaata gtattgaagt atgattatat ggtattcata taatatttga ggaatatcgt
      841 cgccgaacat tttttggcga catgccaatc cattgtttaa atgctttgcc gaaatagaga
      901 ggatcggcat agcccgcttc ataagcaatt tgcttgactt gtttgtcagt tgacaacact
      961 ttacgaagag ctgcgcaaaa ccgcatcttt tgcgtatatg ttcttaacgt tatccccata
     1021 cgctccttaa ataagaagga tatataggat gagcttaatt tgatctttaa tgataaatat
     1081 tctcgatcaa agtcagctcc ctcgtattta ttaatgatct gttcgatttt tgctatttta
     1141 ttcagaggta catgggaaga atatttccca tcttccagcc ctacacagcc aggataattt
     1201 ttaatcaacg aaaaatattc atctggattc gttgaatcga agcctcttaa aacacgaaaa
     1261 gaatagaata tacttttctt ctcgagattt ggaggaagtc ttctaaatat agcaaatctc
     1321 acgataggct tgtttcctaa gcagaaaaac catcgtccgc aataatcggc cgaacaatta
     1381 taatcacaag ccataatgat agcatcgtta ttccttatcc tcgctattgc agctatcgta
     1441 ggattaataa ttaattccca tcggcttcca tcgatattta atttttttat tatagataat
     1501 agggagattg ccgaaggaga ttttatattg ttgcccgcga taataagttt ccctctcttc
     1561 atcttaaaat cccgtttttc tcccttaagg gatacaagat cctttttagc gggcttatac
     1621 aaaaaacact catttattcc cacctgtcat gaatatttat aattatgaat gaaagaaaaa
     1681 aagaaatcaa cgttcttgaa tcacgccgcc taaaatcgtc ccgaagggtg acacgtcccg
     1741 ccatttatga ctcttatcct gtttggacaa agaaaaaagg gatttttccg cgatttccgc
     1801 ggattctttt ctttggtgaa actcccccgg catggggaga aggcgcttgt tcttgatcat
     1861 ctttatcaat cgcgattgcc tgtccgctta agctgcgaaa tagaaataat tccctaagga
     1921 aatattaatg aagaagacgc gcgccgcgat tatcttggcc gccgtttatt atttatcgcc
     1981 aggggggtgc tcccggaatc cgtcgatacc catggagatt atagaaatca cccctcttct
     2041 cgagttgaat gccgttattc cggattataa gatcatgccc ttcttcaatt tatttcagaa
     2101 tatggccatt gatcaacagg gcaatatcta ttacctgaag ttcggccatg acaggatcca
     2161 gaaattcgat aagacgggcc ggctcgcggc cgatatcgga ggaatcggac agggggccga
     2221 cgccttgttc tctccgacgg cgatactggt ggaaaacgat tctctttatg tcctgaatga
     2281 tgcgggccgt caagtaaaga ttttcaacct cgacggcgaa ttcaaatcgt ctttcaagat
     2341 tgacaatgcc tggcccccca cctctctttg cgtcgaccgc gaggccattt acgtgaatgt
     2401 cattgataaa tccaacaaaa cgaggaatga cggcaagctc atttccgtct atgaccatga
     2461 aggaagactc gtcaagaaga tcggcccgat tatcaagtgc aattctatga ttggatataa
     2521 cacgttcaac aggatacgga tgattgcgcg cgacgaaaat atctattacg cctttatcaa
     2581 ccagcccttg atctcctgga gcggaaaaaa cggcggccgg acggccgtct tggatctacg
     2641 gaaattcgga atccccgtca tcgacgcgat cgccgggaga atcgacgcgg aggggattga
     2701 tacgcccgaa acgtttaaag atgattccgt ttcatctgga acttgcatat ctttctgcag
     2761 tggattcgcg gttgatgaag ccggaaggat tttttatatc ccgaatacgg ccggttccgg
     2821 ctcccgtgat gaatgggata attccatttt attattcaat gaaaatcata aattatcaaa
     2881 aaagatatat ttgaaaacgg gaaaagaaac cgtcactccg agagacttct tgatcgccgg
     2941 cggaaaagca atctatggaa ttggaaccat taaagaaagg agcattttat ttaaattcta
     3001 atcgcggcag gattttatgg agaatcgcct ccggaaaatg gggacgcggc gaaacgcgat
     3061 cgcttggatt tttagtctat tcctctggct caccgcttca ggcgcggcca tgattgccca
     3121 tgaggcctgt aaaatcgcgg aaaatcgggc cgctcatgga accctggcgc ttgagctgga
     3181 agaggaagcc gtcattcgcg aatcgaacga gaacgacgat tatcgttttt ctttccttag
     3241 ggacctggcc gcggatgcgg cggaaaattt attcgtcctg gataaaaata gtatcctgaa
     3301 gtatgaccgc cgcggaattt tcttaaaatc attcggccgt aaaggccagg gccccgggga
     3361 attgatggaa ccccggagtc ttttcgtcga cgaacacgac gatctctttg tcaatgatca
     3421 aggcggcttg ctgcacgttt ttgacaagga gggaacattt cggcatcaga tcaaattgga
     3481 tttccaaatt ccctttgatt ccaacgcctt ctacgtaacc ggcgaaggtt tcatctacgc
     3541 tttttcttgg gacatgatca atctggagat gaaaaagact ttcgtgaaag cggattcgag
     3601 cggggcggtc attcttcgcc tgaaaacgat ggatgacgcg gggtgaagat ctcaggaagc
     3661 caaacgggcg gagtcatggg gggaatgatt cacccctatt cggcgaattc ctttttttgc
     3721 ccgatcggca atacgatgtt atgttatgga gaaaacactc gatatcaatt gatactctat
     3781 gatttcgacg gcaacgtgaa atccgtgatg gaccgggatg aaaagccccg gtccatttct
     3841 tcaaaagaaa agaaattctt gggcaagaat tgcgtctttc cttcccatcg cccctttttc
     3901 aagaagttga tgagtgatga caaaggccga atttacgcca taagagtgaa gtcggtttgg
     3961 gatgaaaaca aggccgaaaa ggcggatatt ttcagtcggc acggccgcta tctctaccgg
     4021 acggaattcc ccgcgacgcc ttcgctcatc aaaaacgatt ccgtgtattt cattgacgaa
     4081 ggccaggacg ggctgaaggt catcaagcgc gtgaaaataa ggaattatct ccaaatgaaa
     4141 gaggaataag ggagaccccg tgccaattca ccctaataaa ttttcctttc cgctttatgt
     4201 ttccttttcc gcctgatccg aactaagaat ttatacgaaa aaaatgaggt ccatggggat
     4261 tgtgtctcct cattaatatt gggttataat cacgccgcgc ggggaggcgg ccatggaaat
     4321 catcgatctg gatgagcggt tcgagaacga ttacttcgtc tgcctggagg gctggtcgga
     4381 cgaaccgaag gaagccggcc atcatcggga gacgtggttc cggcggatga aggagaaggg
     4441 tctccgggtc aagatcgccc tcgtcgacgg ccggccggcg ggcatgattc agtatgtccc
     4501 gatcgagcac gccttcgccg aaggccgcga tctttatttc atccactgca tctgggtcca
     4561 cgcttacaag ggaaaaggca tcggggacca gcggaaaaaa ggcctcggca gcgccctcct
     4621 ggcggccgcc gaggaagacg cccgggcgaa ggaggccaag ggcatggtcg cctggggcgt
     4681 cggccttccc gtcttcatgc gggcctcctg gttcaaaaag cacggctatc gcaaggtcga
     4741 caagaacgag gtcgccctcc ttctctggaa gcctttcacc ccggatgccg tcgccccgcg
     4801 gtggatccgg gagaaaaaga gacccgccgc ggtccccggc cggacgtcgg tcgccgtgtt
     4861 ccggaacggc tggtgcacgg cgatgaacat ggtcggcgag cgggcccgcc gggcggccgc
     4921 ggaattcggc gaccgggttg ccttcaccga ggtggacacg tccgaccggg cggcgtatct
     4981 cgaatggggc atcgccgacg gtttgttcat cgacggaaag cccgtccgga ccggcccgcc
     5041 gccctcgtac gataagatcc gccggaaaat cgcccgccgg gtgaaaaaaa taaaatgacg
     5101 tccatgacgc cggccgtccg cgttctcaag gacctcgccg cggatttcga gatcaggctc
     5161 tatcgctacg aagaacacgg cgggacggcc gcggccgccc gcgtcctggc cgtcgaggag
     5221 cacgacgtca tcaagacgct cgtattcgaa accgacggcc ggactccttt tttcgttctg
     5281 atgcacggcg accgggaagt ctcggccaag cgactggccc gcatcctggg agtcaagtcg
     5341 gtcgcgccct gcccgccggc cgccgccgaa cgccacactg gataccaggt cggcggcatc
     5401 tcccccttcg gcgccaggaa aaaccttccg gtctacatgg aacggacgct gaccgaatcc
     5461 ccccgcgttt ggatcaacgc cggcaagcgc ggacaactgg ccggaatggc cccggcggag
     5521 atcctccgga ttctcgctcc cgtcctgatc gaagcggccg tatgaacaac gcggggaggc
     5581 ctccctcagg ccttcgattc atttgaagat tgaggaagcc gatttccaaa aaattgacag
     5641 aaggggaagg gaaattctat actcgctgcc tcttgaagtg aagagaggcc ccttgttgca
     5701 ggagatacaa aagctcaacg aacgcttcgc cgcggccgag gcggaggaca ttctcggctg
     5761 gatggtccgc gcgcttccct accagacggc gatgacctcg tctttccagg cgtccggggt
     5821 cgtcctcatc cacatgctga agaagatcgc cttcgatttt ccgatctatt tcatcgatac
     5881 cggctaccat ttcccggaaa cgctcgagtt caaggcccgc ctgacgcggg agtggaagct
     5941 gaatgtccac accatcgtcc cggaaatgag caaggtcgag cagctcagcc tccacggccc
     6001 ccgcctcttc gaacgggatc ccgacctctg ctgccgcctc aacaaagtcg atccgctccg
     6061 cggcctccag cgggagctcg gattgaaggc ctgggtctcg gctctccgcc gcgaccaggc
     6121 ggaaacccgc cggaatctcc ggccggtgat gatcgacgag agcggcggcc tgcggatcca
     6181 cccgctcctc gcctggaacc gggaacgcgt ctgggcttac atcgaaaaac accgtcttcc
     6241 gacgaatccg ttgtacgagc agggatacgc ctccatcggg tgtttcccgc cgtgctgcac
     6301 ctctaaatcc gaaccgggaa gcgacgaacg gtccggccgg tgggccggga aaggaaaact
     6361 cgaatgcgga ctccacagcc atctcaaaag cgaaaaatag cgggggcggg ctccttgcgg
     6421 aagaaattcg tcctgcgttg cgtcctctgc ggagccgaaa tcgacggatt cggcccttgg
     6481 ttcgaggccg ggcagcgctg ccccgaatgc ggcagcgacc gggcagacgt cgtttaccgg
     6541 accacgcccg ccaaggttcg cgacctcttc cgccggccgg ctaacggcct tccgggcatg
     6601 tggcgctatt tcgacgtcct tccggtcaac gaccgaaaaa acatcgtcac cgccggcgag
     6661 ggcctcgtcc cgatcgaccg gtgggaattc ctcgaggagt atgcccgccg gcgcttccag
     6721 atccgctgcc aagtctacgc ccaccggcaa gacgacaact acgcgaccgg gacgttcaag
     6781 gacctctccg gctcgatcgt ctccagcgtt ctcaaggaaa acggcatcca gaacttcgtc
     6841 gcctccagca ccggcaacac ggccgtggcc tatgcccggt acctgacggc cgccggaatc
     6901 tccttctacg ccttcatccc gaagaacgca tccctgaccc aggcggccga aatcggctgc
     6961 ttcgggcaga acgtcttccg ggtgaaaggc gactacaccc tgaccaagga aatcgccatg
     7021 gccttcgccc ggaaacataa gatgcccctg gccgggggca atttcgatcc gatgcggatc
     7081 gaggcgaaga agacgatgat gcttgaatgg atccgtctgc tcggcaagtt cccgaccgtg
     7141 tatatccaag ccctgagcgg ggggaccggc ccgatggcta tcgccaaggg ctgccgggac
     7201 ctcgccccgg tcgggctggg cggccggatg ccccgcttca ttctttccca gtccagccgc
     7261 tgcgccccca tggccgcggc ttgggcggaa gcgaagaaaa agaaataccc cgagggctgg
     7321 gaaaaaacat acccgatcta ccacaatccc cggaccggca tcgccaccct ggccgcgggc
     7381 tttcccaaga cctatcccgt cctggccccg ctcgtgcggg aaagcggagg ggagatcctc
     7441 tccttcgacg aggacgccac tccgctcatc gcccgcctgg tggcgttcga acgctccgtc
     7501 cggatcgggc cggccgcggc catcggcctc ggcgggttct tcgaggcctt gaagcgcgga
     7561 ctcctccgca acggcgaccg cgtgttgatc aacatcggcg aggggatccg ccgcgcgccc
     7621 gaattcttga tcacgatgtt ccaggaaaaa gccgaagtcg ccacggtcga cgcctgccgg
     7681 cttttcgacc gccgcggaca ccgggccgac atctggaacg agctggaaaa aagggtgatg
     7741 aacggaaaaa ccgcgcccgc ttcccggcgc gggcgctagg attccggttt atcgacgatc
     7801 ccgtacaatc cggccgaggg ccggcgaagg cggcaaactc cgcgcggagg ccgccgggtt
     7861 cctttttcag atcgaggcgg aaggcccggc gttctttcgg tcgcttcttc ggaatcagcc
     7921 gatccaaagt cctcccggtt ttctctgaag tctgaaggat attaggatcc gcggatcacg
     7981 ccggtctttc gcggggggaa gatccatcgc cgccggccgg catcttcaag ggcgatatcg
     8041 cgcgggtgct cttttcgggc ggagagacga aatgaccgtt ccccggaccg gatgatccgt
     8101 cgtccggaaa aagcgttgtc agtccgaaaa caaagaaaag ccccccggcg gaccgggaaa
     8161 ggcgatcact cgtaccggac gagatgaaaa agatcgacgt ccggatgggc tttcctgatc
     8221 gccgggatgc cgccgaggaa ggccagctcg atgaccgccc cgaaaccgac cggcttggcc
     8281 ttgagcatct tgaccaggcg gagggcgctg atcgacgacg agccggtggc gatcacgtcg
     8341 tcgatcacga cgacccgctg gccggcggcg agggcgtcgg cgtgcttctc gaaaaactcg
     8401 acggcgtatt cgttgggcgc ctggacgcag accgtcttgc ggggcagttt gccctttttg
     8461 cggatgaccg acaggcccag gcggaggtcg cgggcgaccg gggcggcaaa gagatatccg
     8521 cgggattcga tgccgacgat gacgtccggc tttaagttcc gggcccaggc ggaaagccgg
     8581 tcgatcgtat aatgatacga ttccggatcc aaaacgagcg gcatgatgtc cttgaacccg
     8641 acgccgggtt tagggaaatc cttgacctcg aggatgcgct gcttgagctg ttcaggtgtc
     8701 atgaatgcct cggacgttga aatacagacg cccttattag acaaatccgc ggcggcgaag
     8761 tcaaacggtt tccccggcgg cggggcgagc cggacgatca gcggtcgccg cgagctgaaa
     8821 acctcgaccg atcaaccgct cgatttcggc cgcacgccgg acgcttgacc cggattgtcc
     8881 gcgttccgtc cggctcgacg ccgatatgga cgcggatgcc gtccaggggg aagtcgatcc
     8941 gctcgcccca ctcgatgagg atgaccgcct cgccgatgtg atcctcgaag cccaggtcgc
     9001 ggatctcggc ctggccgccc agccggtaga ggtccaagtg gaggatcgga acccgtccct
     9061 ggtaaatatt gatcagggta tagctggggc tgcagaccag ccgcggattc ttcatcccca
     9121 agccggccgc gattccccgg gcgaaaacgg ttttccccgc cccgagctcc ccggtcagga
     9181 aaacgacttc gtcgccccgg aaggcggcgg ccaagcgccg ggccgcgaga tacgtccccc
     9241 gctccgtgcg cgtcgtcagg acctggtcct ccgccggggc cggtttcgtc acggctggaa
     9301 caacctcccc ttccggccgc cggccgcgtc ttccagcgct ttcatggccc gggaaagggc
     9361 cttgaggatg tccccggcga ccatcgcccg ctggccgagc tcggcggcgg cgatgtcgcc
     9421 ggccgccccg tgcagataaa cggaggccac ggccgctccg aaggcgttct tttccgcggc
     9481 cagaaacgct ccgagaaggc cgctgagaac gtcgcccgaa ccgcccgtgg ccatgccggg
     9541 attcccggtc gggttgacgg aaacgcgccc gtccgggccg gcgaccagcg tccggtatcc
     9601 cttgaggaca aggaggacac ggtgctccgc ggcgaaccgc gaagccaggg ccagccggtc
     9661 gttcaggacc tcgggaacgg accggccgag cagacgtccg aattcgccgg gatgcggcgt
     9721 caacaccgga ggccgggccg ttttttttaa aaaccccagc ttcccgacaa ggatgttcaa
     9781 gccgtcggcg tcaatgactg tcgggatgcg gacgcccggc aggagcgatc ggaccaaggc
     9841 ggccgtttcg ggatgggtcg aaagcccggg tccgacgacg agggcgtcct tgcccgagag
     9901 cagacgttcc aggcgcggcc gggccgctcc ggcgatcgtc ccctcttccg tttccggaag
     9961 cggctcggtc atcagctcgg acatcgtccg ggcgaccgcg gggacgcagc tttcgggcgt
    10021 cgcgatcgtc accaagcccg cgcccgagcg gtaagccgcc ttgcccgcga gaacggccgc
    10081 ccccgtcttc ccccgcgacc cggccaagat gagaacatgg ccgaacgtcc ccttgtgccc
    10141 gcttctcggc cgcggccgga aaaagggaac ggcgttctcc ggcccggcca tttccagctt
    10201 caattcgggc gaggcgagaa gaacgggcgg cagggaaatc tccgcgacga cgagccggcc
    10261 ggcgtagtcc tcggccggcg ggaagacgtg ggcgattttc ggcgctccga gggcgacggt
    10321 cgcatcggcc cggacggccg gtccgatgac ggcgaacgtg tcggacgaga ggcccgaggg
    10381 caggtcgacg gcgatcgtct cggccggaga ttcgttgatg tccccgatga ccgccgccgc
    10441 aagcccggcg gccggcttgg acagccccgt cccgaagatg gcgtcgatga tgacggtcgc
    10501 ctcccggaga gcccgcttca gattcaggcg ggaccgcccg gcggccgccg tcacctcgac
    10561 gatttcgagg ccgagccgga ggacgatgtc caggttcagg gcggcatcgc ccttgacctc
    10621 gttcttcccg gccaggagaa aaatcctcgg ccgcgccccg aggttgaaca ggtggcgggc
    10681 cgcgacgaag ccgtctccgc cgttgttgcc ccggccgcag acgatcacga tccgctcctc
    10741 cgccaaattc ccgcggagag accggacttc ctcggccacg cggcggccgg cgttttccat
    10801 caggaggaca cccgggatgc cgaactcctc gaacgcccgg cggtcgatct ccctcatctg
    10861 cccggcggtc agtattttca tggaatttcc tcgggccgat tttaccatat ccgccggccc
    10921 gcggctctcc cgtcgacatt ccggcctcgc ggggtcgcgt tgcatttccg gcttcacctg
    10981 tgatactttt caataatcag aaatcgataa tccggttctt tgaggagggc aaccgccatg
    11041 ccgattaaag ttggaatcaa cggcttcggc cggatcggcc ggaacatgct ccgggcctcg
    11101 ggcggttccg ggatcgaatt cgtcgccgtc aacgacatca ccgacgccag gacgctggcc
    11161 catctcctga aatacgactc cgtgctcggg gtttttcccg gggacgtcca gtcgacctcc
    11221 gacacgatca tcgtcgacgg acatccgatc aaggtcctgg cggccaagga tcccggcgag
    11281 cttccctgga aggatctcgg cgccgagatc gtcgtcgaat ccacgggcaa gttcaccaag
    11341 cggcccgacg tcgtcaagca catcgagaag ggaggggccc ggaaggtcat cgtctccgct
    11401 ccggcgaccg atcccgacgt gaccattgtg atgggggtca acgagcggat ctacgatccg
    11461 accaagcatc acatcctctc gaacgcctcc tgcacgacca actgcctggc ccccgtggtc
    11521 aaggtcttgc acgagctggc cggggtcgag aagggcttca tgaccacgat ccactcctac
    11581 accaacgacc agaaaatcct cgacgcgccg cacaaggacc tccgccgggc ccgggcggcg
    11641 gccgtcgccc agattccgac gacgaccggc gcggccaagg ccgtgggcat cgtcctgccg
    11701 gagctgaagg ggaaggtcga cggcatcgct atccgcaccc cgaccccgga cgtctccctg
    11761 gtcgatttcg tcgccgtcgt ccaaaagccc gccccggccg aggtcgtcaa cgaggccttc
    11821 aagacggccg ccgccgggcc cctcaagggc atcctggact acaccgacga gccgctcgtc
    11881 tccgtcgatt tcctccacaa tccgcattcg gcgatcgtcg acggcttgtc gaccaaggtc
    11941 atcgatggcc atctcgtcaa gattctggcc tggtacgata acgagtgggg ttactcctgc
    12001 cgcctcatcg acctggtcaa atacatcgca aggtgagtga aggatgaagc ttatcgaaga
    12061 tctcgacgtc cgcggcaaaa ccgttttcgt ccgggtggat ttcaacgtcc ccctgagcga
    12121 gagcggagag atccggaacg acacccgcat ccgggcgtcg ctgccgacga ttcgtttcct
    12181 cagcgagcac ggggcgaagc tgatcctcgc ctcccacctc gggcgcccta aaaaattcga
    12241 cccggcggcc agcctccgcc cggccgccgt ccgcctggcc gagctcctcg accggaaggt
    12301 ggagatggcc cccgacgtca tcggacccga cgtcgaccgc ctcaaggccg gtctccggga
    12361 cggcggcgtc ctgctgctgg aaaacgtccg cttccagccc ggggaaacga agaacgatcc
    12421 cgagcttgcc cgggagttcg cccggggcgt cgacgtgttc gtcaacgacg cgttcggctc
    12481 gagccaccgg gcccatgcct cggtcgtcgg ggtcgccggg ctcgtcccgg cgaaagccgc
    12541 cggctacctg ttgaaaaagg aagtcgaatt cctcggcaag gtccttcatc cggtcaagcc
    12601 cttcacggcc gttctgggcg gggcgaagac ggaggacaag atcccggtca tcgaatccct
    12661 gctcgacaag gccgacgacg tcctgatcgg cggagcgatg gcctacacgt ttctcgccgc
    12721 ccagggcaag ggcgtcggga aatcgctggt cgagcccgat aagttcgatc tcgccctcgg
    12781 gatcatgaaa aaggccgccg atcgcggcgt ccgtctccgc cttccgtccg accacgccct
    12841 ggcgccctcg atcgattccg gcgtcatcga ccggatgacg ggcgaatatc ccttccccga
    12901 cgtcctcctg ggcgtcgaca tcggccccaa aaccatcgcg acctactcgg cgatcatcgg
    12961 ccgggccaag accgtctttt ggaacggtcc gctcggcgtt ttcgagaatc cggctttctc
    13021 cgagggaacg atgcagatcg cccaggccgt ggccgcctcc ccggcggttt cgatcatcgg
    13081 cggcggcgat tcgatcgcgg ccgtggctaa ggccggcgtc ggcgaccgga tcggccacat
    13141 ctccacgggc ggcggagcga gcctcgaatt catcgccaag ggaacgcttc cgggcctcga
    13201 cgccctggaa ggatgaagac gcggcgatga aaaagcgtcc gttcgtcgcg gccaactgga
    13261 agatgaacat gaccgccggc gagacgcgcg cctggttcga ggcctgggct tccctggccg
    13321 gaacggagga agcggacgtt gtggtctgcc cgccgtatac agccctggcc gaggccgccc
    13381 gccgggccgc cgggatcgcc gttcatctgg gcggccagag cctccactgg gaggacgccg
    13441 gcgcctatac gggcgaaatc tcggggccga tgctggccga cgccggctgc cgatacgtca
    13501 tcatcggcca ctcggagcgg cgccggtact tcggcgagac ggacgagacg gtccggaaac
    13561 ggatcgccgc ggccgtccgg tccggcctgc ggccgatcgt ctgcatcggc gaaaccctgg
    13621 ccgagcggga cgaggggcgg accctggacg tcatccggct ccaattggac ggcggcttgg
    13681 cgggacttac ggaggagacg atcgggaggt tgattttcgc ctacgagccg gtttgggcca
    13741 tcggaacggg ccggacggcc acccccgaac aggcccagga agtccaccgg gccgtccgcg
    13801 gccggctcgc agaaaaaatt ggaaaagcgg cttccgggta tgttataatt ctttacggcg
    13861 ggtcggtgaa accggccaac gcccgcgaac ttttgcggaa accggacatc gacgggtttc
    13921 tcgtcggagg cgcctcgctg gaggcggctt cgttcgggga gatcgtcaag gaatcgatcc
    13981 gggcttacaa ggaagtgaat taacgtgacg gctttattga cgattatcca cgtgattgtg
    14041 tgcctcgtcc tgatcgtggc catccttctc caatccggga aggcggccga cctggccggc
    14101 gcgttcggcg gcggcggaag ccagaccgcg ttcgggccgc ggagcacgca gagcctcatg
    14161 tcgaagatca cgacgatttg cgcggtcgtt ttcatgctca cgtcgctggg tctctggatt
    14221 ttctccggac gcgaagctcc ctcggccgtc aagggcgtga ccgccccggc caagacggaa
    14281 gcgccggcgg cggccccggc ggaaaaaacg cccggacagc aaacgccggc gactgaagtt
    14341 ccgaaacaat aaccgccccg catatgcgcc gaagtggtgg aattggcaga cacgctagct
    14401 tgaggggcta gtgagcattt aaccgctcgt aggggttcga gtcccctctt cggcattcct
    14461 tccccctatc cccggaaggg gatttttcgc cgttttttcg tcgattccct cccggacgat
    14521 cgcagcgatg caattcttga caatcgaccg atttgaattt aagttacgat taaattcagg
    14581 gaatttaccg ggagggaatc atgcttgtca aaaagctcgt cctgtgtttt ttccttgcct
    14641 cactggcatt atcatcaacc ggattctccc aaaacgtaag cctctcaaat gacgcgttcg
    14701 cggtcaaaaa gaagccgccg caaagaaggc cgtcagacgc ggccgagcaa atcgaaaagc
    14761 tcattgagca ggccagggcg gcaaacgcgg atccggccgt tctgcaaaag ctgaccgaat
    14821 cactgaacgc ggcgcggtca atgcctcttc tggcggccgt cccggaaccg gaagaaatca
    14881 tcgccttccc ggccggagcg acatccggcc tcaagccgga aaaggaagaa aaaaccggaa
    14941 acgcgggagg catattcggc ttctactacg ggatgtccga tacattctcg ctttcattcc
    15001 tctacaacat ctcgggagac aataaagtct tcggcctgcc cgaacaattc ggatcgtttt
    15061 tgctgaatcc ctcgtcgaac ggctcctcct ttttagcgac aggcgaatat attttgaaga
    15121 acggagcgaa aacgagcctg gggctgtcgt tccggggagg gatttcattc acggattggg
    15181 aggcgacgat cgccggcgcg gccgtctccc acaagggcca agtctttttt ttcgttccaa
    15241 gcctgcggtt ttgcaccaat gaaaggaact tcggcgacaa caacacgctc aaaatcatcg
    15301 tggacgtcgg gctggggatc agggggatca tcgggaatct cggacaaacg ccggagtttc
    15361 tatcggacaa agccgtcctg ggttgggata aaaagatctt tgtcgggggg gagttgacga
    15421 tctcggccag aatcaacaaa atcacgccct ttatcaggat cacctgttac tcgccgaaat
    15481 cggacattcc gagcttttcg gatttgcagg cttacatcgg aatagacgtc atttccacgc
    15541 tgttttcgtc gaaagtcaat tcgccggagg aatgatccgc ggatcgtcga ttcggacaaa
    15601 tcccgggaat cggttcctcg tcgatccgga ctttctctcc ggccggataa actcatccgg
    15661 cttttcgttt tctattcttc gcgccggaat gccccggcag ctttcaaatc ggactgaacg
    15721 cacacaagac aaatcccccg gattgggcgt atcctgatat cggatatcnn nnnnnnnnnn
    15781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnat caatagtttt tcagcttccc cggaaacgat
    15901 cgatctcggc tccgcatcga ccctatcatg gagcgtttcc aatgctcaaa atgtcgtgat
    15961 cgatcaaggg ataggcacgg ttgattcgac gggatcgata gcggtttctc cgaacgccac
    16021 cgtcacttat acgcttactg cctcaaatgc aaacggagcc gtctccaaat cgtgcctggt
    16081 caccgttaac caaccgccgg taatcaacgg tttcgccgcc actccgaatg tgatcacgct
    16141 cgaatcatcc tcaacgttat cgtgggatgt gtcgaatgcc gttacaagga caatcgatcc
    16201 gggaatcggg caggtttcgg caaccggttc gacgattgtt tccccgaaca gcacgaccgt
    16261 ctatacgctt accgcggtaa acggatcatt tacggtgaca gcgacctgca tgattaccgt
    16321 cgattggcag aatttcatct gctataaaac cgctacggga acaaaatacc atcggggaaa
    16381 ttgccaatac ttaagtcaga gtaagatcca aaccactctc ggcgaggcat gtgctcaagg
    16441 gttgacgcct tgcagtgttt gcaacccgcc ttcttgtaga taacttgggc attattttga
    16501 tgatgcggag ggaatcatga cgggcaaaga gatgaaatcg attctaataa tactcgcatg
    16561 cgcattttta tccgccgctt caatccaaaa acaatcatca cgtttttgca tcaaggttat
    16621 cgacggcgac acgatccttc tcgacggcga tgaaaccgtt cgattaatcg gcgtcgattg
    16681 tttggaaaca aaggatgcgc gtcatcggac acaacctgga tgagcgcact cgtttccccg
    16741 gcgcggtgtt catcaaccgc aagggggtga ccaaggtttc ggtctcctat gcggcgctga
    16801 tgaccggccg gcccgcttcc ggccctccgc tgacttggac atcgaagtac gactcgctca
    16861 cgttcaacgc cttcggccgg gatttccccg acggcggcct gaacgaggcc gggctcttcg
    16921 tcggcgagat gtcctatccg ccgtccaagt ttcccgccga cccggacaag cccgcgatgt
    16981 tcgtcaacct gtggatccag cacctgctcg acacctgcga ctcggtcgac caggtggagg
    17041 cctcggcctc agccctgaac ctggatggct ggggatggca cttcctggcg gccgaccgct
    17101 ccgggggcgc ggcggccatt gagtttctgg acggccgggt cgtcgtccat cgcggcgatt
    17161 ccatgccggt tccggttttg tgcaacgagc cgtacgaata cgaaatgaag aggcgcgaag
    17221 cctatttcgc cgcccgggaa tcaagaacag gctccgccgc ggaggagatt ccgcgcttca
    17281 tccaagccgg agagatgatc gagacttacg gccggcgttc aagcggagag aggaaagagg
    17341 ccgtcgatta tgccttcgac attttagcgg ccttggagcg cggcggaacg cagtggtcgt
    17401 tcgtctgcga tttgcagaac ggggaagcct ggttcaagac cgcggcgact cccaaggtga
    17461 aacacgtcgc cctgggcggg ttcggcggcg gcgacccggc ttcggcgcgt ttcctgaaca
    17521 tggacgccga cttctcgggc gaagcggggt cccgcttcga ggtctattcg ccggcggcca
    17581 accgggaaat cgccgagcgg gcttttgagg ccattctcag gatttcgccg gagttcgaga
    17641 aagtgatcga acgcatcgga gagacgcggg cgagcctcgt cggccgcttc gccggctatc
    17701 ctgaaacgac cagccgctga cgccggccgc cctcccccat caggaaagcg acgccaccca
    17761 gagggagaaa tccgtctccg gcatcgcccc catcgtccgg ccggcttcat ggccgcgccg
    17821 catcacgatg aacgtcggga cggcctggat ttgaaacgcg ccggccgcct ggggattgac
    17881 cgccgtattc acccggacga ccatcaggtt cccggcccgg cgcttggcca gggcttcgac
    17941 caccggatgc atcacccggc agggcgcgca ggtttccgaa tagaaatcga tcagaacggg
    18001 aagagcggcc ttctggatca gaacggccag ttgctcctcg gtcggctcaa gaacctctcc
    18061 cggaacggga agcggcgtct tgcaccggcc gcagacggcc gtttttccga ccgcctcctc
    18121 agggatctgg ttcgtccctc cgcagccgag acagctgatc ttgattttct cgttcacggc
    18181 ttcaccattt tcgtcttggc gagaagggct ttccgctccg tttcgggaaa acgctcgatc
    18241 gcgtaacgga gggaggtccg ggggatcgcc ggcccgtgct tcaggagaaa cgctcgaaga
    18301 cgcggcgcgt cggccttgcc ggcctcccgg agaagccagc cggtcgcctt gtgtatcaaa
    18361 tcgtcttgga ccggaaacag cctgacggcg atctcataaa ccgcgtccaa gtgcgttccg
    18421 cggcgggcga tcttgatgaa cgacacgacc gaggcccgct tcacccaccg gttcgagtcg
    18481 gccgtccatg ttttgatttc cgtcacgaac ttggggtcct tttccagaat cggacccaag
    18541 gcatcgggac agaacacgtc gaccagggcc cagttgtcca gccggtcggc ccgcagccag
    18601 ccgcggaccc gttccggaaa cgaggccggg aattccttgc ggaaccggag gaggatcaga
    18661 agcccgaggg cgcggatctc gtaaaaccgt tccttgagca tcgtttcggc gaaggcgacc
    18721 gcctcgggaa aaccccacgt cccctggacc ttttgccaga cttcggcctc gatttcccgc
    18781 atcttcggcg tcgtcagtcc caggaactcg acctgttcct tgaaataacg gcgctgcccc
    18841 tcggcccgct tggggtcggc gcaggctttc aaagaggcga tcgaggcgcg ggccgcgttc
    18901 tccggccgaa gcgcggccgc gcccgatttt ttatcttctt tcaaacggtt cgtcatgaga
    18961 tgccatcctc ctccccggat ccgtatccgg cgggaaatat taacatccgc ccggcgaccg
    19021 cgtcaacgaa cggcgcaggg accgtcgtcc cttttctccc tcctttacga ggaagagttg
    19081 cgcccgtttt tggcataggc cgccaggtcg agaaaaccgt ggccgctcaa gttgaagaga
    19141 atgaccctct cctgtccgga agcccgggcc tccgccgcct cggtgatcgc cgcggcgacg
    19201 gcgtgggccg attccggagc gggaaggatc ccctcggttt tgaaaaagag ggtcccggcg
    19261 tcaaaagcct cggcctgggg aacggaccgg gattcaacca taccctggcc gaccaggtgg
    19321 ctgacgagag gggccatgcc gtggtaccgc agtccgccgg cgtggatcgg cgcggggacg
    19381 aaatcccggc ccagggtgtg catgaacagg agcggcgtca gaccggccgt gtcgccgtta
    19441 tcgtagcgca gatcgccgcg ggtcagcgaa gggcaggctt cgggctcgac ggcgacatac
    19501 cgcgtcttcc tctcgccggc gagagaaccg cggacgaaag gaaaagccag gccgccgaaa
    19561 ttcgatcctc cgccgacgca gccgatgacg acatccggtt ccgccccggc cgcggacagc
    19621 tgggccgccg cctccaggcc gatgaccgtc tgatgcatta aaacggcatc cagaacggag
    19681 ccgagggcgt atttcgaatt tttcccgccg acggcgacct cgatggcctc ggagatcgcg
    19741 atccccaggc tgccgggatg gtccgggtcc ttctcataaa gcgctcggcc gaaattcgtc
    19801 gatgggccgg ggctttcctc gaccttcgcc ccgaacatct ccatcaccgt ccggcggccg
    19861 ggcttctgcc ggaacgagga ccggaccatg aacaccctga cgtccacgcc gaataaagag
    19921 ccggcctggg ccaaggccga gccccactgg ccggccccgg tctcggtgac gagcgtctca
    19981 accccctccg ccgcggccaa ataagcctgc atcaaggcgg tgttgctctt gtggcttccg
    20041 acagggccga cgccctcgta cttgtagaaa acatgggcgg gcgtgtccag ggcccggcgg
    20101 aggccggagg catagatcag gggggtcggg cggtaaacgg cgagtttttc ccggacctcc
    20161 ttcggaatcg gaatgaaccg ctcccgggtg atttcctggg cgatgaatcc ccgggaaaaa
    20221 acagcctcca ggtccgaagg ccgaagggga gctttcgtcc cgggatgaag aggcgggggc
    20281 ggacaggccg gaaggtcggc cttgatatta tagaaatgtt cgggcaggaa ggcggcgttc
    20341 cgggccgcga accgctcggg ggaaatcgtt ttcgacatgt ctgttctcct ttcagacgtc
    20401 ttcccgacaa ggggaatttg accggttttc aggaacgccg gaacgacggc gcggcgaaac
    20461 gcccgccggc tcccgacgcg cggaaaaagg gtaagggggt ggtattacag ggcggggaga
    20521 tccgcccacc agaacgacac gcggagaacg gcccggaaga gggccgggaa gatgatccgc
    20581 gtgatatggt cgttcatttc ccggcgatga tagacgatcc aaaggtggtt gtcaagaaaa
    20641 aaatatcgaa ggatggggga cggccgcctc agacgatcgc ccctcggcaa gggggccgag
    20701 aatctttccc gcctcgagac gatgccggcg acgatccgtt tcaaacacgg ccggcggaac
    20761 cattacggaa gccggcgggc ttcaattcct cgggatgaag cggagaccgc cggtcgtcgc
    20821 atccggttcg agaaggtaga tatcccattc ctcccgctgc cgattgggcg cctctttttt
    20881 ctggcacatc aagaggttgc ggccgtcggt cctgagattg accgccccgt agaccgagac
    20941 gctccggttg atcagcttgt tttcaagctc ggccagccgg gtttcggcct tgtccctctc
    21001 ccgcgcgccg acatccgccc gatggagagt cgctttcaga tcggcgaatt ccttcttgag
    21061 aacgaaagga tttttcacga tgtcccggga ggaccgggac atcgtgaaaa atcctttcgt
    21121 tc
//
LOCUS       DAOOXH010000036        20905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_2394_length_20905_cov_8.818993, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000036 DAOOXH010000000
VERSION     DAOOXH010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 20905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20905
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2394_length_20905_cov_8.818993"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1853
                     /locus_tag="PKV64_05875"
     CDS             <1..1853
                     /locus_tag="PKV64_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPN16644.1"
                     /translation="MLSNKLRERLPEGFRFQDTGWYQAIEGIHERFLRQLLLTGLSAA
                     AIAAFVYLLLDLRRGNTAYLVYYGIWLAVSLGLLISFLRTGRTFRLATPAVLLLMAGF
                     VLAAFIPPSRDLYLIIFFCFPPIVLHLTGARRGTLWTGVFLLTILLTFVLNTFGFFGP
                     WNIDLTIRQIIVAFVIFSLMFVFSFMNERQKEKFIDQLVRQLSYDKATNLPNREVLIH
                     SIPKDRQFLFAIVHIENFGELGTIFGYETLDEILQFISRYLQQWSREFNYSLYRLRGN
                     DFGLLIPRESMDRAAAESLLNRICTALRREFMAWGRLNIHLNIKLGASFVNDASPVDF
                     LSKADLALKDGIRSHRAVSLYKGDESVRDSAYDSLKLYSTLTRNLEGKTCRAYFQPII
                     DTRTGEVVWFESLLRLRDQDGFYVSPSPFLPLATSTGLDSDITEFMLKEAFTALQKTP
                     CDVSLNVTFKDLLMPEFEKSLFAYLKDAPALNKRLILEIIEREELVENEACRSFLERA
                     RTNGCKIAIDDFGAGYSNLSRLIQFPMDILKIDGSLTRKCLHDEKAKHLIRIVVEYCR
                     SNGIKVVAEHVDRPEIAKFLTGCGVHYLQGNHFGIPQESLTDFDLPALKI"
     gene            1926..2774
                     /locus_tag="PKV64_05880"
     CDS             1926..2774
                     /locus_tag="PKV64_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPN16645.1"
                     /translation="MSGQSKFAAGLAGVFLAITVAAGAACDFMDGGFDDFFDGSKHTE
                     SFSKTLPLKAGDRFALKNTNGYVRVTTWDRNEVEITAQKIASRRKDDLDRVRIEVETP
                     GSDVRVDTVYERFRNVRVKVNYEVKVPAGVILETIRSTNGDIELSGGFGDVKAGTTNG
                     DIRMEGGDGRIVLATTNGTIRARNLRGSVSADTTNGSIYLELDEVRSDIRADTTNGSV
                     NLRLGGEVNADFRARSTNGSITVDFPVTIKGTISSRHRIEGQIGGGGPSIELETTNGS
                     IRVNRM"
     gene            3299..4201
                     /locus_tag="PKV64_05885"
     CDS             3299..4201
                     /locus_tag="PKV64_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16646.1"
                     /translation="MAQTLEFDPGRATLYSALLETLAAGPPAFLFFLQIFGTPGGRFG
                     PVIPSGLGFFALAGAIEGIVRFVINLSSGEAVGSLPLSLLGLRFDKGPRDDVMSDEFL
                     PAGDVLEVLSPTPKAWWLKAGGITCGGDSFLLIRSAAEGRRFRYVFRKGGSGFPETDA
                     VLERERNIAADRAYALAPLWGFLPRADQEAAAFYGRTKTRLWVRGSIAFNLLLSFSIV
                     ALDMVRIGAGGFAWWTLVRFLFGLALLGESVIRLLRHLQTGDVSGSFLGVLIKPLFSL
                     AVQAGPSGAGKPRPPRPGPESSGR"
     gene            complement(4107..6473)
                     /locus_tag="PKV64_05890"
     CDS             complement(4107..6473)
                     /locus_tag="PKV64_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16647.1"
                     /translation="MDDAKAAGRFDGDASRPPGRLFEPVDLFFLFLFLAALLFLFRGF
                     FFQGGVFFERDGAVLEIPARRLAVQLLREGNFALWTDVHGNGQPFLANPKNAVCYPGT
                     WLYLILPFFTAFRFHYLIHALIGWTGMYGLQRFFRLRRPSAFLGASLFFFSGLFLSSF
                     EFYNHVAAFAWMPWILILSFSDSLPRFKKTAFLGILWALQLLAGTPEAVVITLILALG
                     QSFFLPGKAGKRAAVVLASLVLGALLSAVQYLPSLEILGRTERTAAETSLWPLELIQL
                     LNVPFPGILGSDRGAGTSDFWAAHLFDKGAPLYYSFYLGSAGLLLAAFGLAVKTNRAG
                     RGRRWLFLIFLLLASGRYFPLNELLVRVPFLSSIRYPVKYMTGAMFVAAFAAGVFFDD
                     YFVRRRVEARRLRGGWIAALGITAALTALVPVLSRVLGRLFVLRDERLLAAVRGSFYA
                     GLAALSAALVLMGAFSLGKKKSGIFAALFLLLAVADPLIRNSSINPVVPESFYDPAAI
                     LAETGRPVTVYRQEVLPDDMRIKLGGRRAQSFIRQSLYPFSGMSDGVRYVFNRDFFGL
                     HPKEQGELRTAAAGWSEDLLLKHLRSIGCAYLVGPRPLRGLPAEKQIFEGIPLAVQKL
                     GPAASFPYFVGRAVVAKTPAEKKEAFAAEDFDPADAAVVDRPVPGLDGPAAAQAAEAQ
                     AVLEIQGRSVHRMKTPAPVIAVFRGNWDPGWKGRLDGKPVPVFPVNLGLKGVVIPAGA
                     HEVEIRYLPASVLAGAAVSGLTILALAAAGAVFRRRSARPGRPGRTAA"
     gene            complement(6794..7045)
                     /locus_tag="PKV64_05895"
     CDS             complement(6794..7045)
                     /locus_tag="PKV64_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HPN16648.1"
                     /translation="MNIVWTALLGLAAGFVAKLIFPGKENMGILVTMLLGIAGSFLAS
                     YGGQWLKIYQPGESAGFIGSVVGALVILFIYGLIRKRKA"
     gene            complement(7177..8076)
                     /locus_tag="PKV64_05900"
     CDS             complement(7177..8076)
                     /locus_tag="PKV64_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HPN16649.1"
                     /translation="MITSLAQIVKHIKGRTPRRLAVACGEDPHTIEAVGRAVAEGLVQ
                     AVLTGNRSKIESVARDNKIDPASFEIVDEPDPGKALARAVARIKAGESDFLMKGLIDS
                     SLYIKAIIDKEKGLLAPGKLLSHVSVIQVPSRRKLIVCADAAVIPYPDLEAKVQILNY
                     CLAVARKLGIDRPKAAIICAVEKVNPKMVPTTDAAILSKMADRGQIKDADVDGPLALD
                     VALSAESAEIKGVRSPIAGEADVLLFPSIETGNVFFKACSYLAGGETAAVVAGTDCPC
                     VLTSRSDSEDSKFYSIALGALIS"
     gene            complement(8101..9165)
                     /gene="buk"
                     /locus_tag="PKV64_05905"
     CDS             complement(8101..9165)
                     /gene="buk"
                     /locus_tag="PKV64_05905"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPN16650.1"
                     /translation="MRLLILNPGSTSTKIAVYEGDREIFSENIAHSAEQVGRYKRMVD
                     QKDFRKEIILKTLAGRGIDPKSFAAVIGRGGLLKPIPSGTYEVNVRLIEDLAAGIQGE
                     HASNLGGLLADEIARPLGLPAYIVDPVVVDELAGWARLTGFSEIKRRSIFHALNHKYS
                     ARLAAEALGRPYEDLNLIVGHLGGGISIGVHDHGRVIDVNNALNGEGPIAPERAGTMP
                     AADLAALCFSGRFTLDEIKKKIAGKGGMVAHLGTNDLRAVEKRIEEGDASAALVFEAM
                     AKTTAKQIGGAAAVFKGKVDGVVLTGGLMHSAKFVELIRSHISFLGPVFVYPGENEMA
                     ALAAAGGRILRGEEPAKTYN"
     gene            9497..11629
                     /locus_tag="PKV64_05910"
     CDS             9497..11629
                     /locus_tag="PKV64_05910"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPN16651.1"
                     /translation="MISSSSPLGARLLAGIVVLAGAALIAASSRPPVSGASGNPFFAD
                     FDTPFQVPPFDRIKPEHFLPAFEKGMAEQQAAVAAIVADPAPPDYENTIAALDRSDKR
                     LTAVNRVFGGLASADTNDELKAIQKEISPRLAAHRDEINLNKKLFERIKAVYEKRASL
                     GLDAERTFVLENLYKSYIRNGAGLDEAGRAALREINQKLSRLSVQYNQNVLNETNAFR
                     LVVDKEADLAGLPESSLAAAAEAAKAAGLEGRWLFTTQKPSMIPFLTFAQNRALREKI
                     YTAYIKRGDNDNEYDNKKILSDIIKLRVERARLLGYKTYADYVLETRMAKNPQNVYDL
                     LNRLWAAALKVAKKEAGLLQGIIDRENGGFKLAPWDWWYYAEKLRKERYDLDDAELRP
                     YFVLDNVRDGAFWTASQLYGLTFTPLPDIPRPHPDAQAFEVMEADGSPCGVLYLDFHP
                     RAGKNGGAWCGTYRSQNRIFGKDVDPVVTMVMNFSVPSGGRPALLTLEEVETLFHEFG
                     HALNNLLSDITTGTTFRSPDFSELPSQIMEHWAAHPTVVKHYAKHFQTGEAIPDALVA
                     KISNSRLFNQGFDNVEYLAASLLDMKIHTLTEPVDLDINAFEKAYFDSIGLIPEITSR
                     YRSTYFTHIIGGYAAGYYGYIWSGVLDADAFEAFKETSLFDKATAESFRRNVLARNGT
                     ADFMKMFVDFRGREPRIEPLLKNRGLTD"
     gene            11641..12957
                     /locus_tag="PKV64_05915"
     CDS             11641..12957
                     /locus_tag="PKV64_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPN16652.1"
                     /translation="MKRRAVFVPAAFLVLVLTAAGPAAAARPAPSLEKKIKEITEALT
                     PGLIDLRRDIHAHPELGLQEFRTSALVADSFRKLGLEVRTGFATTGVIGILKGGKPGP
                     VAALRGDMDALPLTEETDLPFASREKAVVDGRETGLMHACGHDIHTTILLGVAAVLSA
                     VKADLPGTVVFIAQPAEETVGGAALMIRDGAFRDLVPEAFYAYHVDDTLKAGRIGYTS
                     GFMSANVDGFSLEILSEGCHGASPWLCVDPIVVGAEIVTALQVLISRELNVHNNTVIT
                     VGSFHAGTAANIIPESAKLEATVRNYGEDQRRILREKITRLITNICEAAGAVFTLDYG
                     FGTASVYNDPALTARALTIAERVLGSKEALVEQKPEMGGEDFSAFGTIAPAVMFNLGV
                     VPAGREATAVHSPTFIADEAAIPIGVNLMAEIIVDRLMNPGGKAVK"
     gene            complement(12993..13586)
                     /gene="pyrE"
                     /locus_tag="PKV64_05920"
     CDS             complement(12993..13586)
                     /gene="pyrE"
                     /locus_tag="PKV64_05920"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPN16653.1"
                     /translation="MAPMTNDAVLDLFKKSDALLQGHFKLTSGKHSEWYFEKIRLIEK
                     PAALDRIVDLLVEKIKAEGKEFDYVVSPAYGAIAIGFLAALKLEKKFAFTQRVEEKMS
                     FRSGFSGIDGARAVIIEDILTTGGSIQEVVACLKERNTAIEGIYVLVDRTGGAVSLEG
                     RPVGALLALKIEAFEPEACPFCKSGVPLVKPGASDKK"
     gene            14005..15267
                     /locus_tag="PKV64_05925"
     CDS             14005..15267
                     /locus_tag="PKV64_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HPN16654.1"
                     /translation="MTGSLTLPGSGWKRFWSFIAGAGMITASAMTIRHFYAANFPESI
                     WAGSFCDISAFFNCDSSAFSPIAQFGGVPMGWFGLFVGMLVVLGAVFPTASFERTNKA
                     VSILNAFGVLALLAYSIFIQKSLCLLCSGYYLFSLFSFFLFWRYGIRSERRGLGGFFR
                     TFAAPSLKIAAAAVIGLAAGAYGFREFTLAKKGAQMGGGAANIVRQFNELAVVGDPSF
                     ISPYWIAQAAPRWEDAPIRIVEYADYLCPDCLFLHQQLSRLKAEFPGKMNVAFQFFPL
                     EAACNAVVEKDLHPGACDLSYLAAYDPARFPAFHEEIFAGYPETRKPEWREDFARRHG
                     LEAALTDPAVRDMIRRIIETGAEYEKTSDRYSHGIRSTPTLIIRPLMIRVGHMKAILA
                     SLLEEQAAGPGTGRFLENWVETKPAPKK"
     gene            15290..16567
                     /locus_tag="PKV64_05930"
     CDS             15290..16567
                     /locus_tag="PKV64_05930"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HPN16655.1"
                     /translation="MLKNHPKSLGVLFLTEIWERVGFYTLMAVLVLYMDKTLGWSDSR
                     KGTIYGLFLALCYFVPLLGGWLGDRVFGRRSTVQAGTLLMAAGFVGLAVSSAANTATF
                     YGGLFLIGVGTGIFKVNMAVMVGNLYAGKPELKDAGFNIFYMAVNIGAAVAPLIATFV
                     GVTWNNYNINFWVCAGGLLIASLTFALGRARLAPADLRSEGKKGAAAAPAVAISPSET
                     RQRIATLVTLFIIVILFWVAFYQDFFGLTLFAERSTRIYKFLRPETYQFFEPSFIVIL
                     TPVLLALFARLRTKGKEPSTPTKIFLGMLIMGLAMLVMVFASLAGGDKDANNMSPAWL
                     IGTYFIVTIAEILISPMGQSYVSKVAPPKIAGLMMGGWMAAIAFGSYGSGWLGKYYSD
                     MAHHQFFLLLTGLLVLSAVLVLLFMKRLKRFAS"
     gene            16614..17807
                     /locus_tag="PKV64_05935"
     CDS             16614..17807
                     /locus_tag="PKV64_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPN16656.1"
                     /translation="MLIKEKIHQAAELLREFGCDCWLTFVRETAVNGDPVLPFLVPGD
                     LTWHSAFLVGRDGRTRAIVGRYDRQMVVDTGAYDDVTSYVTGFREPLQAVLKELNPRT
                     IAVNFSEGSEICDGLTHGMYLTLEKTLEEIGMADRLVSAEKLVSALRERKSAAEISRI
                     REAIRHTEEIYALVAPFIAPGKTEKDIADFMYAEIVRRGLPTAWSRETCPAVYTGPDT
                     AQAHYNPTGRIVRPGHLLNMDFGVKVDHYCSDMQRTFYVLEKGETEAPVDVQKGFETL
                     LRSIEAAREAIRPGAAGRDVDAASRAVLAAAGYEEFPHALGHQVGRFAHDGTAILGPA
                     WEKYAQKPFRTLEPGMVFTIEPRLTVPGRGVATIEDMILVTETGSEWLSHPQTELILV
                     GRESR"
     gene            17804..>20905
                     /locus_tag="PKV64_05940"
     CDS             17804..>20905
                     /locus_tag="PKV64_05940"
                     /inference="COORDINATES: protein motif:HMM:NF004799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPN16657.1"
                     /translation="MTTVERAPRFEPRDAEAFARTLFGFPGSAAPLPSERDQNFLLTS
                     ASGERRILKIANPDENPEFLDLQNAAMGHLGSGGDPLPVPAVISDTRGRSIAEIEDKN
                     GVPHAVRMVTFIEGTPLGTVHPRPLELLADLGGILGRLTRRLASFDHPAARRDFRWDL
                     QNGERIVRENLPLIGDPARSGLAVFLLERMTAILAPLWDRLPRSVVYNDANDFNVIVG
                     PPEAGAGTFGRRRIAGLIDFGDMVRSVTTAEPAVACAYAMLGADEPLPAAAALAAGYH
                     SVQPLDDVEIQALYPLAVLRLLMSVAICARQSGARPDNAYLQISNEAAWNLLDRLRSV
                     HPRFAESIFRRACGRSPHPAAGRLRDWLALTEAAGGFAPVMGVPLKTASRTVFDLSVG
                     SPLFPNPETVDDTVGWARLLSEKMKAQKAVFGLGRYGEARIVYTTKNFRPAGRPYAEG
                     RTVHLGIDVFAAAGTPVFAPFEGTVIFARDNAERLDYGPTIILEHRADAAGPGGGPLV
                     FWTLYGHLGRASLEGMTPGRAVKKGERIGTVGTMPENGGWPPHLHFQVILDLFDEQGN
                     YPGVCRASEWDVWRAICPNPNLILGLEPAETAETGRTPEEILAVRRERLGPSLSVAYR
                     KPLKIVRGFMQHLYDHTGRMYLDAVNNVPHVGHSHPRVVAAVQRQAAVLNTNTRYLHD
                     TLARYIERLTATLPPALRVCYLVNSGSEANDLALRLARHFTGRRDMIVLGGAYHGHLT
                     SLIEISPYKYDAPGGPGRPAHTQAAEMPDVYRGLYRADDPEAARKYADSVSSCLDRIA
                     AAGRLPAGFIAESLLSCGGQIVLPDGYLQDVYRRVRKAGGVCLADEVQVGFGRVGTHF
                     WGFQTQGVVPDIVTMGKPIGNGFPLAAVVTTRAIADAFASGPEYFNTYGGNPVSLAAG
                     TAVLDVLEQENLQERALRVGSVLRAGLQRLQLDHSLIGDVRGLGLFLGIELVSDRGKR
                     IPAAAEAAYVAERMRAEGILFSTDGPDHNVLKIKPPLCFSSADAERLVETLGRVLDED
                     PLRR"
BASE COUNT         3736 a         6740 c         6546 g         3883 t
ORIGIN      
        1 ggatgctttc caataaactc cgggaaaggc tgccggaagg gttccggttc caggacaccg
       61 gctggtacca ggcgattgag gggattcatg aacggttcct tcgccagctt cttctgaccg
      121 gattgtcggc ggccgcgatc gccgcgttcg tttatctttt gctcgatctg agacggggaa
      181 acaccgccta cctcgtctat tacgggatct ggctcgccgt ctccctcggg ctgctgattt
      241 ctttcctcag gacggggcgg acgttccggc tggcgacccc ggccgtcctt ctgcttatgg
      301 ccggattcgt cctggccgct ttcattcctc cttcccgcga cctttacctc atcatctttt
      361 tctgttttcc gcccatcgtc ctccatctga ccggcgcccg ccggggaact ctctggacgg
      421 gcgtcttcct cctgacgatc ctgttgacgt tcgttctgaa cacgttcggg tttttcgggc
      481 cctggaacat cgatttgacc atccggcaga tcatcgtcgc cttcgtcatt ttcagcctga
      541 tgttcgtttt cagcttcatg aacgagcggc agaaagaaaa attcatcgat cagctggttc
      601 gccagctgag ctacgacaag gccaccaacc tgccgaaccg cgaggtgttg atccattcca
      661 tcccgaaaga ccggcagttc ttgttcgcca tcgtccatat tgaaaacttc ggcgagctgg
      721 ggacgatctt cggctacgaa acgctggacg agatcctcca gttcatttcc cgctatctcc
      781 agcaatggag ccgggagttc aactattctt tatatcggct gcgcggcaac gatttcggcc
      841 tcctgatccc ccgcgaatcg atggaccggg cggcggccga atccctgctc aaccggatct
      901 gcaccgccct ccgccgcgaa ttcatggcct ggggcaggct gaacatccat ttgaacatta
      961 agctcggggc ctcgttcgtc aacgacgcgt ccccggtcga tttcctgtcc aaggccgact
     1021 tggccctcaa ggacggcatc cgctcccacc gggccgtttc cctttacaag ggcgacgaga
     1081 gcgtccggga ttcggcttat gattccctga agttgtacag caccctgacc cggaatctcg
     1141 aggggaagac atgccgggct tattttcagc cgatcatcga tacccgcacc ggggaagtgg
     1201 tctggtttga aagcctcctc cggctgcgcg accaggacgg cttttacgtc tcgcccagcc
     1261 ctttcctccc cctggccacc tcgaccggcc tcgattcgga catcaccgag ttcatgttga
     1321 aggaggcctt tacggccctg cagaagacgc cctgcgacgt ttccctgaac gtgacgttca
     1381 aggatctctt gatgccggag ttcgagaaat ccctctttgc ttatctcaag gacgctccgg
     1441 ccttgaacaa gcggctgatt cttgagatca tcgagcggga agagctggtc gagaacgagg
     1501 cctgccggtc gttcctcgag cgggcccgga ccaacggctg caagatcgcc atcgacgact
     1561 tcggagcggg atattccaat ctcagccgct tgatccagtt cccgatggac atcctgaaga
     1621 tcgacggcag cttgacccgg aagtgcctcc acgacgaaaa ggccaagcat ctgatccgga
     1681 tcgtggttga gtattgccgg tccaacggca tcaaggtcgt cgccgagcat gtcgaccggc
     1741 cggaaatcgc caagttcttg accggctgcg gcgtccatta ccttcaaggg aaccattttg
     1801 ggattcccca ggaatcgctg acggacttcg atcttcccgc cctgaaaatc tgagttttct
     1861 ccccggggaa cctttttcac ccgaaatgcg tcttttccgg tgaacgtctc ggaggaggat
     1921 cgaacatgag cggacaatca aaattcgccg ccggcctggc gggcgtattc ctggcgatca
     1981 ccgtcgccgc cggcgccgcc tgtgatttta tggacggcgg cttcgacgat tttttcgatg
     2041 gatcgaagca caccgaatcg ttctcgaaaa cccttcccct aaaggcggga gaccgattcg
     2101 ccttgaaaaa cacgaacggg tatgtccggg tgacgacttg ggaccggaac gaagtcgaga
     2161 ttacggccca gaaaatcgcc tcccgccgga aagacgactt ggaccgggtg aggatcgagg
     2221 tcgaaacccc cggctcggac gtccgggtgg acacggttta tgagcggttc cgcaatgttc
     2281 gagtgaaggt gaattacgag gtgaaggtcc ccgccggggt catcctcgag acgatccgct
     2341 caaccaacgg cgacattgag ctttcgggcg gcttcggcga tgtcaaagcc ggcacgacca
     2401 acggcgacat ccggatggag ggcggggacg gccggatcgt cctggcgacg accaacggga
     2461 ccatccgcgc ccgcaacctc cggggatcgg tttcggccga cacgaccaac ggctcgattt
     2521 acttggagct ggacgaggtg agaagcgata tccgggcgga cacgaccaac ggctcggtca
     2581 acctgcgcct gggcggcgag gtcaacgccg actttcgggc ccggtcgacg aacggatcga
     2641 tcaccgtcga ctttcccgtc accatcaagg gcacgatttc ttcccgccac cggatcgagg
     2701 gtcaaatcgg cggaggcggc ccctccatcg agctcgaaac gaccaacgga tcgatccggg
     2761 tcaaccggat gtaaaggccg ccgggaggct cccggagcct tcccttgttc cgataagcgg
     2821 gcggcttccg gccgtccaac cttcgcggcc ggaagccgtt ccgcggtttg atcccccccg
     2881 gccgtccgga tcggggccgc tcgttgacac cccgcgaaag ccggggatat agtgtctttc
     2941 ggcattccga tgaaatcgaa catccgtgag atccgccttc ctcatcccca tccggagatg
     3001 aggccccggg tcgaaggctc cccccggacg gctcctcatc cagggacggc ggtccggatc
     3061 ggagaagatc tgttcgaggt gatgaccgaa acatgctccg ccggccgaga atggatctac
     3121 gggctggagc catggaaggc gggcaacgtc acgcgcatcg tcgtcgagtg gggggacgca
     3181 gccgagaaag acttcctggc cggcctccgg agggaacgga tccggacgcg caaaaaaaaa
     3241 cctggctttg ggcggccagg ctctgttggg atttctcccc cgtccttttc aggaacgctt
     3301 ggctcaaacc ctcgaattcg acccggggcg ggcgacgctc tattcagccc tcctggaaac
     3361 gctggccgcc gggccgccgg cttttctctt cttcctccag atcttcggaa ccccgggcgg
     3421 gcggttcgga ccggtcattc cctcaggctt gggatttttc gccctggccg gcgcgatcga
     3481 gggaattgtc cggttcgtga tcaacctctc ttcgggggaa gcggtcggaa gcctcccctt
     3541 gtcccttttg ggtctccgct tcgacaaggg gccgagggac gacgtgatga gtgacgagtt
     3601 cctgcccgcc ggcgacgttc tcgaggtcct ctcgccgacg cccaaggcct ggtggctgaa
     3661 agccgggggg atcacctgcg gcggcgattc cttcctgctt atccggtccg cggccgaggg
     3721 ccgtcgtttc cggtatgtct tccggaaagg cggctcggga tttccggaaa cggacgcggt
     3781 tctggagcgc gagcgcaaca tcgccgccga ccgggcgtat gcgctggcgc cgctctgggg
     3841 tttcctgccg cgggcggacc aggaagcggc ggccttctat ggccggacca agacccggct
     3901 ttgggtccgc ggctcgatcg ccttcaacct tctgctttcg ttttcgatcg tcgcccttga
     3961 tatggttcgg atcggggccg gaggattcgc ctggtggacg ctcgtccggt tcctgttcgg
     4021 cctggctctc ctcggggaaa gcgtcatccg ccttctccgt catcttcaaa ccggtgatgt
     4081 gagcgggagt tttttgggtg ttttgatcaa gccgctgttc tccctggccg tccaggccgg
     4141 gccgagcggc gccggaaaac cgcgcccgcc gcggccaggg ccagaatcgt caggccgctg
     4201 acggccgccc cggcgagaac gcttgccggg agataacgga tttccacttc gtgagcgccc
     4261 gccgggatga cgactccctt gagcccgagg ttgacgggga aaaccgggac gggcttcccg
     4321 tcgaggcggc ctttccagcc cgggtcccaa tttccgcgga aaacggcgat caccggggcg
     4381 ggcgttttca tccggtggac ggaacggccc tggatttcga ggacggcttg ggcttcggcg
     4441 gcttgagccg cggccggtcc gtccaggccg ggaaccggac gatcgacgac ggccgcgtcg
     4501 gccgggtcga aatcttcggc cgcgaaggcc tctttttttt cggccggcgt tttcgcgacg
     4561 accgcccttc cgacgaaata cggaaacgag gcggccggcc cgagcttctg gacggcgagg
     4621 gggattccct cgaagatctg cttctccgcc ggcagtcccc gcaacggccg cggcccgacg
     4681 agatacgcgc agccgatcga ccggagatgt ttgagcagga gatcctcgga ccagccggcc
     4741 gccgccgtcc tcagctcccc ttgctccttc gggtggaggc cgaagaaatc gcggttgaaa
     4801 acatagcgga cgccgtcgct catcccggaa aacgggtaga ggctctggcg gatgaagctt
     4861 tgggctctcc ggccgccgag cttgattctc atgtcgtcgg gcaggacttc ctggcggtac
     4921 acggtcaccg ggcgccccgt ttcggcgagg atggcggccg gatcatagaa ggattcgggg
     4981 acgacgggat tgatggaact gttccggatc agcggatcgg ccacggcgag gaggagaaac
     5041 aaggcggcga agatccccga tttctttttc cccaggctga aggcgcccat caggacgaga
     5101 gccgccgaca gagcggccaa gcccgcataa aaggaaccgc ggaccgcggc gaggagccgc
     5161 tcgtcgcgga ggacgaagag gcgtcccagg acccggctta agacggggac gagggcggtc
     5221 aaagcggccg ttattcccag ggccgcgatc catccgcccc gaaggcgcct cgcctcgacc
     5281 cggcggcgga cgaaataatc gtcgaaaaac actccggcgg cgaaagccgc gacgaacatc
     5341 gccccggtca tgtatttcac cgggtagcgg atcgaagaaa gaaagggaac gcggacgaga
     5401 agctcgttca aggggaaata ccggccgctg gccaggagaa gaaaaattaa aaacagccat
     5461 ctccggcccc gcccggcccg gttcgttttg accgcgagtc cgaacgcggc caggagaagc
     5521 ccggcgcttc cgagatagaa gctgtaataa agcggcgcgc ccttgtcgaa gagatgggct
     5581 gcccaaaaat cagacgtccc cgccccccgg tccgatccca ggatcccggg gaaggggacg
     5641 ttgagcaact ggatcaactc cagcggccag agggacgttt ctgcggccgt ccgctcggtc
     5701 cgtcccaaga tttccaggga ggggagatac tggacggccg aaagcagggc gcccagaacg
     5761 agggaggcca ggacgactgc ggcccgcttt ccggccttgc cggggagaaa aaacgactgg
     5821 ccgagggcga ggatgagagt gatcacgacc gcttccggcg ttccggcgag gagctggagg
     5881 gcccaaagaa tccccagaaa agccgttttt ttgaatcgcg gaagggaatc cgaaaaggag
     5941 aggatcaaga tccagggcat ccaggcgaaa gcggcgacat gattgtagaa ttcaaagctc
     6001 gaaaggaaaa ggccgctgaa gaagaaaagc gaggcgccca gaaaggccga cggccgacgg
     6061 aggcggaaga accgctgaag cccgtacatt cccgtccagc cgatcaaggc atggatcaag
     6121 taatgaaaac ggaaggcggt gaaaaagggc aggatgagat agagccaggt tcccggataa
     6181 cagaccgcgt tcttgggatt ggccaggaac ggctggccgt tgccgtggac atccgtccag
     6241 agggcgaagt tgccttcgcg gaggagttga acggccagcc ggcgggcggg aatctcgaga
     6301 acggcaccgt cccgctcgaa gaaaacgccg ccctggaaga aaaatccgcg gaagagaaaa
     6361 agcagggcgg ccagaaacag gaaaaggaaa aaaaggtcaa cgggttcgaa caggcgcccg
     6421 ggaggccggc ttgcgtctcc atcgaaccgc cccgccgctt tcgcgtcgtc catcggccga
     6481 agcataatgc atccccgaag gcgatgtcaa aaccggcgat tgtccttctc tcccgcccgg
     6541 ccgttttttc gaagagcgtg attcccggcc ggggaacggt ctctcgaaac gtgcgcggag
     6601 gttccggatg ccgtgaccgg ccccccctcc gccccgcggc ccagattttt ctcaagaagc
     6661 ggggggacgc gccgggccga cgccgccgtt tcggaatccc gccgatcgcg gggattcccg
     6721 caccggccgc catcccggaa tcagccgcgt cggcggccgg cccggccgtt gggagccgcc
     6781 ggaaaaaacg gccctatgcc ttgcgctttc tgatcagccc gtagatgaac aggatcacca
     6841 gggcgccgac gacggagccg atgaagccgg ccgattcccc gggctggtag attttgagcc
     6901 actggccgcc gtaggaggcg aggaacgagc cggcgatacc caacagcatc gtcacgagga
     6961 ttcccatgtt ttccttgccg ggaaagatca gcttggccac gaatccggcg gccaaaccca
     7021 gaagcgccgt ccagacgatg ttcatgcgat ccccttcctt tccgggaaag agaggcggtt
     7081 aaaaccgctc tttcccgcga tcccgtcgat tccccctgaa aataattaaa aagcggcggg
     7141 ctgtcaagga cgagaacgca gctcggcggc tgtgcgtcag gagatgaggg cgcccagggc
     7201 gatcgaatag aatttcgaat cctcggaatc cgaccgggat gtcaaaacgc aggggcaatc
     7261 cgtgccggcc acgacggccg ccgtctcccc tccggccagg taggagcagg ccttgaaaaa
     7321 gacgttgccc gtctcgatgc tcggaaagag caggacgtcg gcttccccgg cgatcggcga
     7381 gcggacaccc ttgatctcgg cgctttcggc ggacagggcg acgtcgagag ccagcgggcc
     7441 gtcgacgtcg gcgtccttga tctggccgcg gtcggccatc ttgctgagga tcgcggcgtc
     7501 ggtggtcggg accattttcg gattcacttt ttccacggcg cagatgatcg cggccttggg
     7561 ccggtcgatc ccgagcttcc gggcgaccgc caggcaatag ttcaggatct ggactttcgc
     7621 ttccaggtcg ggataaggga tgacggccgc atcggcgcag acgatgagct tccgtcgcga
     7681 cggcacctgg atgacgctga catgggacag aagcttgccc ggggcgagaa gccctttttc
     7741 cttgtcgatg atggccttga tgtaaaggga ggagtcgatc agccctttca tcaggaaatc
     7801 ggattcaccg gccttgatcc gggcgacggc ccgggccaag gctttccccg gatccggctc
     7861 gtcgacgatc tcgaacgagg ccgggtcgat cttgttgtcc cgggcgacgc tttcgatttt
     7921 cgaccggttg ccggtcagga cggcctggac caggccttcg gcgacggccc ggccgacggc
     7981 ctcgatcgta tgaggatcct cgccgcaggc gacggccagc cgccgcggtg tccggccttt
     8041 gatgtgtttg acgatttgag cgagcgatgt gatcatcgtg agttcctccg tgcggacgtt
     8101 tcagttgtat gttttcgccg gctcctcgcc tctcaggatc cggccgccgg cggcggccaa
     8161 ggcggccatc tcgttttcgc cggggtagac gaaaacggga ccgaggaagg aaatgtgcga
     8221 tcggatcagc tcgacgaact tggcgctgtg catgaggccg ccggtcaaaa cgacgccgtc
     8281 gaccttgccc ttgaaaacgg cggccgcgcc gccgatctgc ttcgccgtgg ttttcgccat
     8341 agcctcgaag accagggcgg ccgaggcgtc gccctcctcg attctttttt ccacggcgcg
     8401 gaggtcgttg gtccccaagt gggcgaccat tccgcccttg ccggcgatct ttttcttgat
     8461 ttcgtcaagg gtgaaccggc cggaaaagca gagagcggcc aggtcggcgg ccggcatcgt
     8521 tccggcccgc tccggggcga tcggcccctc gccgttaagg gcgttgttga cgtcgatgac
     8581 ccggccgtgg tcgtgaactc cgatcgagat cccgccgccg agatggccga cgatcaagtt
     8641 gaggtcttca tacggccggc ccaaggcctc ggccgccagc cgggccgaat atttatgatt
     8701 gagggcgtgg aagatgctcc ggcgcttgat ttcggaaaat ccggtcaggc gggcccagcc
     8761 ggcgagctcg tcgacaacga ccggatcgac gatataagcg ggaaggccca gcggccgggc
     8821 gatctcatcg gctaggagcc cgcccaggtt gctggcgtgc tccccttgga tcccggccgc
     8881 aagatcctcg atcaaccgga cgttgacctc gtaggtcccg ctcggaatcg gcttgagcag
     8941 ccccccgcgg ccgatgaccg cggcgaagct cttgggatcg atcccgcggc cggccaaggt
     9001 tttcaggatg atttccttgc gaaagtcctt ttggtcgacc atccgcttat accggcccac
     9061 ctgttccgcc gagtgggcga tgttttcgga gaagatttcc cggtcgcctt cgtagacggc
     9121 gattttcgtc gaagtcgatc cggggttgag gattaaaagg cgcatgattc caccttggca
     9181 tgggattcgg aaaaaccgcg agcccaatat agaaaagccc ggccgggctg tcaataaggt
     9241 cccgttttcg tcccggtttc gaatgagaag acctctgatg attcacgccg gcccgaggga
     9301 gacgttctcc cgtcgaatct ccggagggaa gcccaggtct tctcctttct tgggatagga
     9361 tgagttcccg ggttttctcc tcccgcccgg ctccgagccg ctccggccgg ctcgtttcct
     9421 tgacacatcc gctcggcggg acgtatcatt tttctccttt gaataaatca ccgtccgtca
     9481 tgaagggaga tcacccatga tcagctcgtc ctctccgctc ggcgcgcggc ttctcgccgg
     9541 gatcgtcgtc ctggcgggag cggccctcat cgccgcctcg tcccggccgc ccgtttccgg
     9601 ggcgtccggc aatccgtttt tcgccgactt cgacacgccg ttccaggtcc ccccgttcga
     9661 ccggatcaag ccggagcatt ttcttccggc gtttgaaaag ggcatggccg agcaacaagc
     9721 ggcggtcgcg gcgatcgtcg ccgatccggc tccgcccgat tacgaaaaca cgatcgccgc
     9781 cctggaccgg tcggacaagc ggcttaccgc ggtcaaccgg gtgttcggcg gattggcctc
     9841 ggccgacacg aatgacgagc tcaaggccat ccagaaggag atctcccccc gcttggccgc
     9901 ccaccgggac gaaatcaacc tcaacaaaaa gctgttcgag cggatcaagg cggtgtatga
     9961 gaagagagcg tccctcggac tcgacgccga gcggaccttc gtcctggaga acctgtacaa
    10021 gtcctacatc cgcaacggcg ccggcctcga cgaggccggc cgggcggcgc tccgggagat
    10081 caaccagaag ctgtcccggc tgagcgtcca atacaaccag aatgtcctga acgagaccaa
    10141 tgccttccgg ctggtcgtgg acaaggaggc cgatctggcc ggtctgccgg aatcgtccct
    10201 ggcggccgcg gccgaggcgg ccaaggcggc cggcctggaa ggccgctggc tcttcacaac
    10261 ccagaagccg agcatgatcc ccttcctgac gttcgcccaa aaccgcgccc tgcgggaaaa
    10321 gatctacacc gcctacatca agcgcgggga taacgacaac gagtacgaca acaagaaaat
    10381 cctgtccgac atcatcaagc tccgggtcga gcgcgcccgt cttcttggat acaagactta
    10441 cgccgattac gtcctggaaa cgcggatggc caagaacccg caaaacgtct atgacctcct
    10501 caaccggctg tgggcggcgg ccctcaaggt cgccaagaag gaagccggcc tgcttcaagg
    10561 gatcatcgac cgggaaaacg gcggattcaa gctggctccg tgggactggt ggtactacgc
    10621 cgaaaagcta cggaaggaac gttatgacct cgacgatgcc gagctccggc cctacttcgt
    10681 cctggacaac gtccgggacg gcgcgttctg gacggccagc cagctctacg gcctgacctt
    10741 cactccgctt ccggacatcc cgcggcccca ccccgacgcc caggccttcg aggtcatgga
    10801 ggcggacggc tcgccgtgcg gcgttcttta cctggatttc cacccccggg cgggcaagaa
    10861 cggcggagcc tggtgcggaa cgtaccgcag ccagaaccga atcttcggca aggacgtcga
    10921 tcccgtcgtg accatggtca tgaatttttc cgtgccctcg ggaggccgtc cggccctgtt
    10981 gaccctggag gaggtggaga cgctgttcca cgagttcggg cacgccctga acaacctctt
    11041 gtccgacatc accaccggaa ccaccttccg gtctccggac ttctccgagc ttccgtccca
    11101 gatcatggag cactgggccg cccatcccac ggtcgtcaag cattacgcca agcatttcca
    11161 gacgggggag gccatccccg acgccctggt cgccaagatc tccaacagcc gcttgttcaa
    11221 ccagggcttc gataacgtcg agtacctggc cgcctccctc ctggacatga agattcatac
    11281 cttgaccgaa ccggtcgacc tcgacatcaa cgccttcgag aaggcgtact tcgattcgat
    11341 cggattgatc ccggaaatca ccagccgcta ccggagcacc tatttcacgc acatcatcgg
    11401 ggggtacgcg gccggctatt acggctacat ctggtccggg gtcttggacg ccgacgcctt
    11461 cgaggccttc aaggaaacga gcctgttcga taaagccacg gccgagtcct tccgccggaa
    11521 cgtcctggcc cgcaacggga cggccgactt catgaagatg tttgttgatt tccgcggccg
    11581 cgagccccgg atcgagcccc tgcttaaaaa ccggggcctg acggattgag cggggaatcc
    11641 atgaagcgcc gcgccgtttt cgtcccggcg gccttcctcg tcctcgtttt gaccgcggcc
    11701 ggaccggcgg ccgcggcccg accggcgccc tcgctcgaga agaaaatcaa ggagatcacg
    11761 gaagccctca cgcccgggct catcgacctc cgccgggaca tccacgccca tcccgagttg
    11821 ggcctccagg aattccgcac ctcggccctg gtcgccgatt ccttccgcaa gctcggcctc
    11881 gaggtccgga cgggattcgc gacgacgggc gtcatcggga tcctcaaggg agggaaaccc
    11941 ggtccggtcg cggccctgcg cggcgacatg gacgccctcc ccctgacgga ggagacggat
    12001 cttcccttcg cctcccggga aaaagcagtc gtcgacggcc gggaaacggg cctgatgcac
    12061 gcttgcggcc acgacatcca cacgacgatc ctcctcggtg tcgccgccgt cctttcggcc
    12121 gtcaaggccg atcttcccgg cacggtcgtt ttcattgccc agccggccga ggaaaccgtc
    12181 ggcggagccg ctctcatgat ccgggacggg gcgtttcggg atctcgttcc cgaagcgttt
    12241 tacgcctatc acgtcgacga cacgctcaag gccggccgga tcggatacac ctccggattc
    12301 atgtcggcca atgtcgacgg cttcagcctg gaaatcctct cggagggctg ccacggggcc
    12361 agcccctggc tctgcgtgga tcccatcgtc gtcggagccg agatcgtcac cgccctccag
    12421 gtcctgatct cccgggagct gaacgttcac aacaacacgg tcatcacggt cggctccttc
    12481 catgccggca ccgcggccaa catcattccc gagtcggcca agctcgaggc caccgtccgc
    12541 aattacgggg aggaccagcg ccggattctc cgggagaaga tcacccgtct catcacgaac
    12601 atctgcgagg cggccggggc ggtcttcacc ctcgactatg gattcggcac ggcctcggtg
    12661 tacaacgacc cggccctgac ggcccgggca ttgacgatcg ccgagcgcgt gctcggatca
    12721 aaggaggccc tcgtcgagca gaagcccgag atgggcgggg aggatttcag cgccttcgga
    12781 acgatcgccc cggcggtgat gttcaacctc ggcgtggttc ccgccggccg ggaagccacg
    12841 gcggttcatt ctccgacgtt catcgccgac gaggcggcca tccccatcgg cgtcaacctc
    12901 atggccgaaa tcattgtcga ccgcttgatg aatcccggcg ggaaggctgt aaagtaagtc
    12961 cgaagggggc gcggggggct tgcctcccgt ccctattttt tgtccgaggc cccgggtttg
    13021 acgaggggga cgccgctttt gcagaacggg caggcctccg gctcaaaggc ttcgatcttg
    13081 agggctagga gcgcgccgac gggccggcct tcgagggaga cggctccgcc ggtccggtcg
    13141 accaggacgt agatgccctc gatcgccgtg ttccgctcct tgaggcaggc gacgacttcc
    13201 tggatggacc cgccggtcgt caagatgtcc tcgatgatga ccgcccgggc gccgtcgatc
    13261 ccggaaaagc cggatcggaa cgacatcttt tcctcgaccc gctgggtgaa ggcgaatttt
    13321 ttttcgagct tgagggcggc caggaagccg atggcgatgg ccccgtaggc gggggagacg
    13381 acatagtcga actccttccc ctcggctttg attttttcga ccagcaggtc gacgatccgg
    13441 tcgagggcgg ccggcttctc gatcagccgg atcttctcga aataccattc cgagtgcttg
    13501 cccgacgtga gtttgaaatg gccctggagc agggcgtcgg atttcttgaa cagatcgagg
    13561 acggcgtcgt tcgtcatggg agccatcggt tcctccggga gccgcggacg gcccgctgtt
    13621 ttggaatttc tttccctcgg cgtttccgct ttccgccggg ggagggaccc gattatagag
    13681 agaacggggc gatttctcaa ggaccgttcc gctgcggcgc ccgcctcctc ctcccgtgcc
    13741 gtgattttat ctgtcgcggc ttcatcggat ccgcgattca ggttttcttc aaggaagccg
    13801 gaagccctga agaaggcggc gatttccgtc gatcgcgtat tcctcaaggc gtgaagaaac
    13861 cgccggtcaa ggagtcgcga cggtccccgc ggaatcgagg ccggcgcggc ctccggcgga
    13921 cgcccgtttg actgatcaag ggacatcggt ccgggatttt gttaaaatac gcccggttcg
    13981 ccgcaagacg acaggaggca atccatgacc ggttcgttga ctctccccgg aagcgggtgg
    14041 aagcgattct ggagcttcat cgccggggcc ggcatgatca ccgcctcggc catgacgatc
    14101 cgtcatttct acgcggccaa ttttcccgaa tcgatctggg ccggctcctt ctgcgacatc
    14161 agcgcgtttt ttaactgcga cagctcggcc ttttccccga tcgcccagtt cggcggcgtg
    14221 ccgatgggct ggttcggcct cttcgtcggt atgctcgtcg tcctgggggc ggttttcccc
    14281 acggcttcct tcgagcggac gaacaaggcg gtctcgattc tcaacgcgtt cggggtcctg
    14341 gccctcttgg cttactcgat cttcatccag aaaagcctct gcctgctgtg cagcggctat
    14401 tatctgtttt ccctcttcag cttctttctc ttctggcggt acgggatccg gagcgagcgc
    14461 cggggcctcg gaggattttt ccggacgttc gccgcgccgt ctttgaaaat cgccgccgcc
    14521 gccgtgatcg gcctggccgc cggggcctac ggcttccggg aattcaccct ggctaaaaaa
    14581 ggagcccaaa tgggcggcgg cgccgcgaac atcgtccgtc agttcaacga attggccgtc
    14641 gtcggcgatc ccagcttcat ttcgccgtac tggatcgccc aggcggcgcc gcgctgggaa
    14701 gacgccccca tccggatcgt cgagtacgcc gattatctct gcccggactg cctcttcctc
    14761 catcagcagc tctcccggtt gaaggccgag ttcccgggaa agatgaatgt cgctttccag
    14821 ttcttccctc tcgaagccgc ctgcaacgcg gtcgtcgaaa aagatctcca tcccggggcc
    14881 tgcgacctgt cgtatcttgc cgcgtacgac ccggcccgct tcccggcctt ccacgaggag
    14941 attttcgccg gctatcccga gaccaggaag cccgaatggc gggaggactt cgcccgccgt
    15001 cacgggttgg aagcggccct gaccgatccg gccgtccggg acatgatccg gcggatcatc
    15061 gagaccgggg ccgagtatga aaagacgtcc gatcgataca gtcacggcat ccgttcgacg
    15121 ccgaccctga tcatccggcc gttgatgatc agggtcggcc atatgaaagc gatccttgcc
    15181 tcgcttctgg aggaacaggc ggcgggcccg ggaaccggcc ggttcctcga aaactgggtc
    15241 gagacgaagc cggcgccgaa gaaataatct ccgacgaaag gcggatctca tgctgaaaaa
    15301 ccatccgaag agcctgggcg ttcttttcct gaccgagatt tgggagcggg tcggtttcta
    15361 cacgctcatg gccgtcctcg ttctctacat ggacaagacc ctcggctgga gcgattcccg
    15421 gaagggcacc atctacgggc tgttcctggc cttgtgctat ttcgttcccc tcctcggcgg
    15481 ctggctcggc gaccgggtgt tcggccgccg ctcgaccgtt caggccggca ccctgctcat
    15541 ggccgcggga ttcgtcggtc tggctgtctc ctcggccgcg aacacggcga cgttctacgg
    15601 cggcttgttc ttgatcggcg tgggaaccgg catcttcaag gtcaacatgg cggtgatggt
    15661 cggcaatttg tatgccggga aaccggagct caaggacgcc ggcttcaaca ttttttacat
    15721 ggccgtcaac atcggggcgg ccgtcgcccc cctcatcgcc acgttcgtcg gggtgacctg
    15781 gaacaattac aacatcaact tctgggtttg cgccggcggg ctgctgatcg cctcgttgac
    15841 gttcgccctc ggacgggcga ggctggcccc ggccgacctg cggtcggaag ggaagaaggg
    15901 agcggcggcc gcgccggccg tggcgatctc cccctcggaa acccggcaaa ggatcgccac
    15961 cctcgtcacc ctgttcatca tcgtcatcct gttctgggtg gccttctacc aggatttctt
    16021 cggcctgacg ctgttcgccg agcggtcgac gagaatctac aagttcctcc ggccggaaac
    16081 ctaccagttc ttcgagccgt ccttcatcgt catcctgacg ccggtcctgc tggccctttt
    16141 cgcccggttg agaaccaagg gcaaggagcc gtcgacgccg acgaaaatct tcctcgggat
    16201 gctgatcatg ggcttggcca tgctggtcat ggttttcgcc tccctggccg gcggggacaa
    16261 ggatgccaac aacatgtcgc ccgcctggct gatcgggacg tacttcatcg tcacgatcgc
    16321 cgagatcctg atctcgccga tgggccagtc ctatgtctcg aaggtcgccc cgccgaaaat
    16381 cgccggcctg atgatgggcg gctggatggc cgcgatcgcc ttcggaagct acggatcggg
    16441 ctggctggga aagtattaca gcgacatggc ccaccaccag ttcttcctgc tgctgaccgg
    16501 gctcctggtg ctctcggccg tgctggtttt gttgttcatg aagcgcctca agcggttcgc
    16561 gtcctgacgc cggccgcggg ggcgggcggc ccgggccgcc ggaggagata tccatgttga
    16621 tcaaggaaaa aatccatcaa gccgcggaat tgctccgtga attcggctgc gattgctggc
    16681 tgacgttcgt ccgggaaacg gccgtcaacg gggatccggt cctgccgttt ttggtcccgg
    16741 gcgacctgac ctggcattcg gcgttcctcg tcggccgcga cggccggacc cgggcgatcg
    16801 tcggcaggta cgaccgccaa atggtcgtcg acacgggggc ctacgatgac gtcacttctt
    16861 atgtcaccgg gttccgcgag cctcttcagg ccgtcctgaa ggagctgaat ccccggacga
    16921 tcgccgtgaa cttttccgag ggcagcgaga tctgcgacgg cctgacccac ggcatgtacc
    16981 tgaccctgga gaaaaccctg gaggagatcg ggatggccga ccgcctcgtc tcggccgaaa
    17041 agctggtctc cgccctccgc gagcggaaat cggccgccga gatcagccgg atccgcgaag
    17101 ccatccggca caccgaggag atctacgccc tggtcgcccc cttcatcgct cccggaaaga
    17161 ccgagaaaga tatcgccgat ttcatgtatg ccgagatcgt ccggcgcggg ctgccgacgg
    17221 cctggagtcg ggaaacctgt ccggccgtct acaccggtcc cgacacggcc caagctcatt
    17281 acaacccgac cggccggatc gtccggcccg ggcatcttct caacatggat ttcggggtca
    17341 aggtcgatca ttactgctcg gacatgcagc ggacgtttta tgtccttgaa aaaggggaaa
    17401 ccgaggcccc ggtcgacgtc caaaagggat tcgagacgct cctccgctcg atcgaggccg
    17461 cccgggaggc gatccggccg ggcgcggccg gccgcgacgt cgacgccgcc agccgggctg
    17521 ttctcgcggc cgcgggctac gaggaatttc ctcatgccct cggccaccag gtcggccggt
    17581 tcgctcacga cgggacggcg atcctgggac cggcctggga aaaatacgcc cagaagccgt
    17641 ttcggacgct cgagcccggc atggttttca cgatcgagcc gcggctgacc gttcccggcc
    17701 gcggcgtcgc gacgatcgag gacatgatcc tcgtgaccga gacgggatcc gaatggctat
    17761 cccatccgca gaccgagctg atcctcgtcg ggcgagagag tcgatgacga cggtggagcg
    17821 ggcgccgcgg tttgagccgc gggacgccga ggccttcgcc cggacccttt ttggttttcc
    17881 gggatcggcc gcgccgcttc cgagcgagcg cgaccagaac tttcttctca cgtccgcctc
    17941 cggcgagcga cggattctga agatcgccaa cccggacgaa aatcccgaat tcctcgacct
    18001 ccaaaacgcg gcgatgggac acctgggatc cggcggcgat ccgcttccgg tcccggccgt
    18061 gatttccgac acgcggggcc ggtcgatcgc cgaaatcgag gacaaaaacg gcgtccctca
    18121 cgccgtccgg atggtcacct ttatcgaggg gacgccgttg gggacggtcc atccccgacc
    18181 gctcgagctt ctagcggatt tggggggcat cctcggccga ctgacccgac ggctggcttc
    18241 cttcgatcat ccggccgccc gccgggattt ccgttgggat ctgcaaaacg gcgagcggat
    18301 cgtccgggag aaccttccct tgatcggcga tcccgcccgg tccggcctgg ccgtctttct
    18361 cctggaacgg atgacggcga ttctggcgcc cctctgggac cgcctgccgc ggagcgtcgt
    18421 ttacaacgac gccaacgatt tcaacgtgat cgtcggaccg cccgaggccg gggccgggac
    18481 gttcggccgg cggcggatcg ccggcttgat cgatttcggc gacatggtcc gctcggtgac
    18541 aacggctgag ccggccgtgg cctgcgcgta cgccatgctc ggggcggacg agccgctccc
    18601 ggccgcggcc gccctcgccg ccggttatca ttccgtccag cctctggacg acgtggagat
    18661 ccaggccctc tatcccctgg ccgttctccg gctcttaatg agcgttgcga tctgcgcccg
    18721 gcagtcgggc gcgcgtccgg acaacgcgta tctccagatc tcgaacgagg ccgcctggaa
    18781 tctgctcgac cgcctgcgga gcgtccatcc acggttcgcc gaatcgattt tccgccgggc
    18841 ctgcggccgc tctccccatc ccgcggccgg ccggcttcgc gactggcttg cgttgacgga
    18901 ggcggccggg gggttcgccc cggtgatggg ggttcctctc aaaaccgcct cgagaaccgt
    18961 cttcgattta agcgtcggat caccgctttt tccaaatcct gaaaccgtcg acgacaccgt
    19021 cggctgggcc cgtcttttat ccgagaaaat gaaagcccaa aaggccgtct tcggcctcgg
    19081 ccggtacggc gaggcccgga tcgtttacac gacgaagaat ttccggccgg ccggccggcc
    19141 gtacgccgag ggccggaccg tccatctcgg aattgatgtg ttcgccgccg ccgggactcc
    19201 cgtgttcgct ccgttcgagg gaacggtgat cttcgcccgg gacaacgccg aacgcctgga
    19261 ttacggcccg acgatcattc tcgaacaccg ggccgacgcg gccgggcccg ggggggggcc
    19321 gctggttttc tggacgctgt acggccacct cggccgggcg tcccttgaag ggatgacgcc
    19381 cggccgggcg gtgaaaaaag gcgaacggat cgggacggtg gggacgatgc cggaaaacgg
    19441 cggttggccg ccgcatctcc actttcaagt gatccttgac ttgttcgacg aacaggggaa
    19501 ttatcccggc gtctgccggg cgagcgaatg ggacgtctgg cgcgcgatct gtcccaaccc
    19561 gaacctgatt ctcgggctgg agccagccga gacggctgag acgggacgga cgccggagga
    19621 gatcctggcc gtccgccggg aacgcctcgg cccctccttg agcgtcgcct accggaaacc
    19681 cctcaagatc gtccgcgggt tcatgcagca cctgtatgat cacaccggcc ggatgtacct
    19741 ggacgcggtc aacaacgttc cccatgtcgg gcattcccat ccccgcgtcg tggccgccgt
    19801 ccagcgccag gccgccgttc tcaacaccaa cacccgctat ctccacgaca ccctggcccg
    19861 gtacatcgag cggctgacgg cgaccctgcc gccggctctc cgggtctgct acctcgtgaa
    19921 ctcgggcagc gaggccaacg acctggcctt gcggctggcc cggcatttca ccggccggcg
    19981 ggacatgatc gtcctggggg gggcgtacca cgggcatctg acctccttga tcgagatcag
    20041 tccgtacaaa tacgacgccc cgggaggccc gggccggccg gcccacaccc aggcggccga
    20101 aatgcccgac gtgtaccgag ggctataccg ggcggatgat ccagaggccg cccggaaata
    20161 cgcggactcc gtctcgagtt gtctcgaccg gattgcggcg gcgggccgcc tcccggccgg
    20221 attcatcgcc gaatccctcc tcagctgcgg agggcagatc gttcttcccg acggatacct
    20281 tcaggacgtt taccggaggg tccggaaggc cggcggcgtc tgcctggccg acgaggttca
    20341 ggtcggattc ggccgggtgg gaacgcattt ctggggcttc cagacccagg gcgtcgtccc
    20401 cgacatcgtg acgatgggca agcccatcgg caacggcttt cctctggccg cggtcgtgac
    20461 aacccgggcg atcgccgacg ccttcgcctc ggggccggaa tatttcaaca cgtacggcgg
    20521 aaatcccgtc tcgctggcgg ccggaacggc cgtcttggac gtcctcgagc aggagaatct
    20581 ccaagaacgg gccctccgcg tcgggtcggt gctgagggcc ggtttgcagc ggctccaact
    20641 cgatcactcc ctgatcggag acgtccgcgg tctcggcctg tttctcggca tcgagcttgt
    20701 ctcggaccgg gggaaacgga ttccggcggc ggcggaagcg gcctatgtcg ccgagcggat
    20761 gagggcggag gggatccttt tcagcacgga cgggccggac cacaacgtcc tgaaaatcaa
    20821 gccgccgctt tgtttctcct cggccgacgc cgagcgcctc gtcgaaacgc tcggccgcgt
    20881 cctggacgag gatcccctgc gccgg
//
LOCUS       DAOOXH010000037        20526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_2457_length_20526_cov_8.022910, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000037 DAOOXH010000000
VERSION     DAOOXH010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 20526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20526
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2457_length_20526_cov_8.022910"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PKV64_05945"
     CDS             complement(<1..636)
                     /locus_tag="PKV64_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN16658.1"
                     /translation="MSNVLTVRGLKKSYGAHAALAGIDFEVRAGEVFALIGPNGAGKT
                     TTLRIAATLLTSTAGEITFLDRDLRKEPDFVREKISYLPEEAGAYKDMKGMDYLRFMA
                     GFYGRDAGEEKEFVERAAAITELGNRLKTKVGTYSKGMTRKLLLARALMTRPVLAILD
                     EPTSGLDVLNALDVREIIRRYVKEGTSVLLSSHNMLEIEFLSDRVGLIDKGR"
     gene            complement(766..2178)
                     /locus_tag="PKV64_05950"
     CDS             complement(766..2178)
                     /locus_tag="PKV64_05950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPN16659.1"
                     /translation="MKFQKVMKFGGGCLRDADGFRRAAAVIRREPVPPAVVVSAVSGI
                     TDELERGLARAVKNEAGIARTIAALRLQNDRLASGLLSAREIRAEIAAQIGHRLARLE
                     RLLRGAALIGEISPYARAHVLSFGERWAALLLAGILREEGADARALESEQIGLAADDV
                     PEGATADLAAFRRAVRKNALGIEAGRFVPVITGFYGLAAGGRLVSFGRNGSDYSAAVV
                     AAALRVSLLEIWKDVDGFMTADPRRVSGAKPVARLSFTEAAELSYFGAKVLHPRALEP
                     IARENGTIWIRHLDRPDRPGTEIAAEGGTAETVLKSVTLNDRIAVLRVSGPGVGFRPG
                     VIGDIGRSLAERNVNILSVITAQTAINILVAEEDARRARRILRSVRRGVVTRVDLETN
                     LALVAAVGEGLSSERGLAARVFAAVAGNGVNVEMISSGASGAAAYFLVRKADALPAVR
                     ALHAEFFETRAGRPRALRCS"
     gene            complement(2189..3256)
                     /gene="asd"
                     /locus_tag="PKV64_05955"
     CDS             complement(2189..3256)
                     /gene="asd"
                     /locus_tag="PKV64_05955"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPN16660.1"
                     /translation="MNKYPVAVLGATGLVGQHFVRMLEGHPDFEIALLAGSERSTGRP
                     YAAAVEWTAGGDLPSQAGKTTVQPASVEAVIASGARVVFSALPASAAGAAEDALQSRG
                     LAVFTNASARRGDPDVPVVVPEINPDHLVRAARRKTNRGGFIAADSNCSTAGLALLLA
                     PLRPFGIRSVAVTTFQAISGAGRRGLAAMDIAGNCVPFIRGEEEKLAREPGRIFGTLE
                     ADGFIPWPVPVRASCCRVPVREGHLLSVSVEFEGAVGEDAAAEALASFRGLPQEMGLP
                     SAPERPVIVRTEDNRPQPALDVWAGTPARARGMAVTAGRLRSRGRILDLFGLVHNTVR
                     GAAGACLLTAELAARQGLLHE"
     gene            complement(3296..4021)
                     /locus_tag="PKV64_05960"
     CDS             complement(3296..4021)
                     /locus_tag="PKV64_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HPN16661.1"
                     /translation="MAEALFRKRLRIKETAATIVAAEPFLPLAEADIRKQRGRLEDYI
                     RRDPGFRTALAPRSVPPEAPPIVRRMARAASRAGVGPMAAVAGALAESALRAMAEAGA
                     THALVDNGGDIALLLERPVTVGIFTGPSPVRDIAFRVEPRPGIFGICASSGTVGHSLS
                     FGRADAAVVLSADVGLADAVATALGNAVTDEDETRLRETLAALGGIEGVEGLLAVVGK
                     TLAVWGDLPPIVRTRVDPARISR"
     gene            complement(4014..5171)
                     /locus_tag="PKV64_05965"
     CDS             complement(4014..5171)
                     /locus_tag="PKV64_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HPN16662.1"
                     /translation="MPKTIAEINEKIARKTVKVVRADHMSRLVRELGPEKAAENVDVV
                     TTGTFGPMCSSGVWLNFGHSEPPIKMTRVRLNGVEAYAGAAAVDAYLGATQLSASRGV
                     RYGGAHVIEDFLRGRPVILQAVSPGTDCYPRKRLLTVLTLNDLNTALLSNPRNGYQRY
                     NAAANSSSRTLHTYMGTLRPRLGNVTYAGAGSLSPLSNDPDYRTIGLGTRILLGGAEG
                     FVTGPGTQHDPQNGFGTMAVQGDLKRMSPDFLRAALFPGYGCTLCIGLGVPIPILDAD
                     SARRAAVSDAEIAVTIRDFGLPSRDRPALRSVTYAELRSGRVRLGGRIVPALPLSSLV
                     LSRRIADELQRRIEAGTFLLTAPVVPLPAESVVRPLEIRRPKTSAPGEHHG"
     gene            5732..7168
                     /locus_tag="PKV64_05970"
     CDS             5732..7168
                     /locus_tag="PKV64_05970"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPN16663.1"
                     /translation="MSKRRTKRIVLVVLFLILAAAVFLVIKNQYFQMMSIGTGSKAKI
                     LCSSVFLSGRDATAVEAEDLGFHPLYKIFKTKVDYEQKRVSCSLLGTGLFKAEAVFRE
                     GIGAVLLAGGALEEVVRAWPKPEIDAAVEETDPESVDWPMGDRLILVPPGTTIDAARI
                     RAAGDRLFSEADPNHPLRTRALIVLHDGRLVYERYGPGITKNTRLLSWSTVKSVTCVL
                     AGILVGRGRLDLDLPVPVEAWAGAGDSRRTITLDQLLRMSSGLEWFEAYADRPVSDVN
                     DMLFRKPDMAAFAASKRRAVPAGAAWNYSSGTANIVQGILRKTLGSDEEYWNFPRRAL
                     LDRIGMRSAVMERDASGTFVGSSYLYATARDFARFGLLCLNDGVWLGERILPEGWMAY
                     ATAPAPAAPQGRYGAFFWLNRGDPGKPETRPYPLLPEDAYFALGYQGQTIAVLPSQKL
                     VVVRLGMTYDDDWGGMNVFLPEIAAAVK"
     gene            complement(7187..8071)
                     /locus_tag="PKV64_05975"
     CDS             complement(7187..8071)
                     /locus_tag="PKV64_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPN16664.1"
                     /translation="MKQTEIIILAAAIIVFFFLIGIRIVRPTHRGLVERLGRYKRFAN
                     PGFNWIIPVIDRLYQVNVTEVMVDAQPQEIITNDNLNARVDAQVYFKVKNDEQSVKNS
                     QYNVNNYQWQIVNLARTTLRNIIGTLTLKSANSERGKINSELQKTLREETGSWGIEIV
                     RTELKEIDPPKDVQETMNKVVKAENEKIAAVDFATATETMADGARRAEIKRADGVKQA
                     RILAAEGEAQAIKLVNEAAQAYFIGNAQVLKRMEVAEKALAHNAKIVVPADTQLINVI
                     GEMAGLVPLPPAPESVQK"
     gene            8511..10130
                     /locus_tag="PKV64_05980"
     CDS             8511..10130
                     /locus_tag="PKV64_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPN16665.1"
                     /translation="MKSGKTAAYFLIAAAAFLFPSSCVNRRREAPPPSSSSLSSSSSF
                     SSAAARNYALIEPGESIDSIIRKAAAVVPSPRQWAWQAREFMGFIHFGINTFTGREWG
                     DGTEDPALFNPADFDARQWVRVFKQAGMKTLILTAKHHDGFCLWPTAETAHGVKSGPW
                     RGGRGDVVGEVAAACREAGLGFGFYLSPWDRHEPSYGDSPRYNAFFLAQLRELLTGYG
                     PIAEVWFDGAPGDGPERKNQIYDWPAYYRTIRELQPEAVIAMMAPDARWVGTESGYGR
                     ETEWSAIPVDVRNPESLAAASGLFPLDDVFVPRDLTAADLGSREKLAGARVLAWYPAE
                     TDVSIRPGWFYHASQDGDVKSPEKLLDIYESSVGRNGVLLLNVPPDRRGLIHENDEEA
                     LRELRRSLDAAYGTNLAAGAAVRASASATGHGPETMLDDDLNTFWTPPGEGGRAEITI
                     DLPGPSEFDRFVLREHIRSGQRIESYVLEAGEGNAWRPVARGTTVGAMRILRFPAVSA
                     RRLRLVIEQSRAAPALASLGLHRVRTESGPR"
     gene            10127..10708
                     /locus_tag="PKV64_05985"
     CDS             10127..10708
                     /locus_tag="PKV64_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HPN16666.1"
                     /translation="MNNVEIKARVRDLDAFAARLKALRPSKPRVLVQEDVFFKIPRGR
                     LKLRILGPRTGELIYYERPDAAGPKLSSFEIARTSDPAGLRAVLAAAFGVRGVVRKIR
                     LAARAGQTRIHLDAVSGLGLFAELEVVLRRGQAAADGAAVARALMKKLKIRPADLVSG
                     AYLDLLEKASGPAVSPSAKTACDRKPSGNTPSA"
     gene            complement(10647..11429)
                     /gene="nagB"
                     /locus_tag="PKV64_05990"
     CDS             complement(10647..11429)
                     /gene="nagB"
                     /locus_tag="PKV64_05990"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HPN16667.1"
                     /translation="MEVIIKPDSHAASRLAARKIAALVRAKPDAVLGLATGQTPLETY
                     DRLSELHRTEGLDFSRVTTFNLDEYLGLEPSHPASFHACMRTSLFSKINIDPARVFLP
                     DGMSEDIPRTCREYEERLRAAGGIDLQLLGIGSDGHLGFNEPSSSLSSRTRIKTLTDR
                     TRKDNAAAFGGEDNVPHHVITMGLGTIMDARTCLLLAFGRKKAAAIAQAVEGPVSAMV
                     PASILQMHPRAIVILDEEAASGLKRTEYYRWVYDHKPSWQKD"
     gene            complement(11506..12291)
                     /locus_tag="PKV64_05995"
     CDS             complement(11506..12291)
                     /locus_tag="PKV64_05995"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HPN16668.1"
                     /translation="MTIPPAPAFKNRLKKGDLLTGILLSLPSPEIAETCASAGFDWLF
                     LDMEHGLLGFSDVQRMVQAVSGRSACLVRVAVNDPAWIAKALDTGADGLIFPHLNQAA
                     EARAAVKAARYAPQGSRSIGLGRAQGYGARVAQALNEANDKIVLVAQAEHIEAAENIS
                     DILSVEGLDAVFVGPFDLSASLGVPGRTDAPLVRGAIARIREACAGRGLPSGIFSPDA
                     AAARAAAAEGFTLLCVSTDTLLLDHAAWNLLREVRAEARSADR"
     gene            12380..13978
                     /gene="murJ"
                     /locus_tag="PKV64_06000"
     CDS             12380..13978
                     /gene="murJ"
                     /locus_tag="PKV64_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPN16669.1"
                     /translation="MADIARGVRSFAVGTGLSRILGLVREQVFAFLFGVGAASDAFNA
                     AFRIPNLFRDLFAENALSNAFVPVLTAARAKGREEENRFASNILNTLLLTVGLIVVLG
                     MIFSPALARVVGLGFGAIPGKTALTARLTFLMFPFLLFIVLAAWAMSYLNTENEFFIP
                     AVAPAFFNLFSIIVPVVLFGWFVNRGEDPILGMAFGVTAGGFVQFLVQVPSLSRRGYA
                     YRLHLNFRDAAFRKAMALFVPVAIGLSASRINVLVNTMLISVIPGGISWLNYAYRVFH
                     LPLGMLGVSVGQVSLPLFSRLVNERRFDDLRRTLDDSLRLVLFLTVPTSVLIAVLARP
                     IIAVIYQHGLFTAADTRATAVMLVLYLIGAPFVSALRNLTSVFYACRDARLPMIASFI
                     SVGTNIAFNLALMGVLGHRAFPLSASIAAVVNFAILLALLPRKIGSLPAGPLAGFAVR
                     LVLASAAGGGAAWVGAAAAARIAGTSFWSGLGAVAVCGTAGLGIFYAVCRILGLDEAR
                     DFARRLLRRGRSEDAAPPAGTDPV"
     gene            complement(14086..14742)
                     /locus_tag="PKV64_06005"
     CDS             complement(14086..14742)
                     /locus_tag="PKV64_06005"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPN16670.1"
                     /translation="MRFQAVIFDLDGTLLDTIEDLTDSMNAALARLGFPAKTVEECKI
                     LVGDGLATLIRRALPSSRAEDPEAFRKLYDGMRAEYRLRNAVKTRPYPGIPELLAALA
                     ERKIPLAVLSNKPHDSTREVVEKNFPGVPFRFVCGAREEIPVKPDPAGALDIARRFHI
                     PPGEILYVGDTNTDMRTAAAAKMFAVGVLWGFRTADELRKNGAQVLVSRPSEILGLLD
                     "
     gene            15403..15852
                     /locus_tag="PKV64_06010"
     CDS             15403..15852
                     /locus_tag="PKV64_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPN16671.1"
                     /translation="MLAIKKILCPVDFSEPSLLGLSYSAELARLFEADIEVIYVLPIL
                     PPQPIDPQFSFQVPEYEILLHRDAETRLAALVKEKIPEGIKAVPVVGHGSAGKEIVKR
                     AEETKADLIVISSHGHGGWHHLVMGSVAERVIRHSPCPVFVVREARK"
     gene            16071..16142
                     /locus_tag="PKV64_06015"
     tRNA            16071..16142
                     /locus_tag="PKV64_06015"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16104..16106,aa:Glu,seq:ctc)
     gene            complement(16182..16931)
                     /locus_tag="PKV64_06020"
     CDS             complement(16182..16931)
                     /locus_tag="PKV64_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16672.1"
                     /translation="MKRTFLIIGFLIFLFACLWAGAFPSAAAEQETPETSGLQELRLR
                     VSFTAEVSPSPVSGRIIIGFHRDPSAPIDNPDLLDPQPTFAWDVRGWKPGEAIILEGR
                     DAVCWHGDLSALDGWYGVQASLKANRMARTLQAPGNARSVKNVVFIEKGRMSRPLDLL
                     IHVPAGPAPEFKESEFIKEANVPSPRLTRFYGKAQSIRAAVILPPGYFQNPERRYPSV
                     YVMGGWGSSPLDALGGAPQKRYGIKTLGGRG"
     gene            17341..18132
                     /gene="mtgA"
                     /locus_tag="PKV64_06025"
     CDS             17341..18132
                     /gene="mtgA"
                     /locus_tag="PKV64_06025"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HPN16673.1"
                     /translation="MSGTENGSPRPKKKHVFGKAAAAGAALLIVFAGMVWLTLPDVAD
                     LKTENPKSTALIERRKADARAQGKTLVVRIHWIAFDRIPPLLKKSVLVSEDASFYDHQ
                     GVDFAELKASFKKDLKEGRFVRGGSTITQQLAKNLYLSPAKSPVRKLREFLIAQRLER
                     TLPKNRIFHLYLNVIEWGPGIFGAAAAARHWFGKDVAALTLEEMVRLTAVIPRPLRSD
                     PRKNDRWMRFKGRWIADTLRTVGAIGGDDHQALVRAFEESRSPLP"
     gene            18433..19860
                     /locus_tag="PKV64_06030"
     CDS             18433..19860
                     /locus_tag="PKV64_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPN16674.1"
                     /translation="MGGFFGVASRAENISDLFYGTDYHSHLGTKRGGLAVVRPEGLVK
                     SIHNIENTQFRAKFDPDLGGLTANLGIGVISDSDNQPIVIHSHLGRFAVVTVAAIKNM
                     DALVRRVFRERRIHFAESMGGGVNPTELAAALINQEDSFEAGIRNLQDSIEGSCSVLV
                     LTEKGIYAGRDKYGRTPVILGAKPGAWAVTLETCAFPNLGFETVRELGPGEIVFITPE
                     GAETRQKPGGLLQICAFLWVYYGFPASTYEGINVEAARNRCGEALARRHPAAVDFAAG
                     IPDSGVGHGIGYAAAAGIPFKRPFVKYTPTWARSFMPQNQDVRDLVARMKLIPIKELI
                     QGKRFLFCEDSIVRGTQLKDTVQRLYDTGAMEIHMRPACPPLIHGCKFLNFSVSRSEM
                     DLAARRAIREIEGEKPVDLSLYADERSPQYEAMVERIRRRLNLTSLRFQRLEDLIWAI
                     GLPKDRLCTYCWDGVEVKSPSLPFA"
     gene            complement(19977..20050)
                     /locus_tag="PKV64_06035"
     tRNA            complement(19977..20050)
                     /locus_tag="PKV64_06035"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20016..20018),aa:Gln,seq:ctg)
     gene            20140..>20526
                     /locus_tag="PKV64_06040"
     CDS             20140..>20526
                     /locus_tag="PKV64_06040"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPN16675.1"
                     /translation="MIRKQWFRLFFVLGLTGVLLYVFLRGVDWSEAFRYLKEHVRWGM
                     FLLVIPLSQIHILTRAWRWKILMVHEKKDVRLYNLAAANAVGFTVSYLFPGRIGELVK
                     PLYLARQEKCRSGFAIGTCVVERIFDV"
BASE COUNT         3432 a         6562 c         6800 g         3732 t
ORIGIN      
        1 ccggcccttg tcgatgagtc cgacccggtc ggagaggaac tcgatctcga gcatgttatg
       61 ggaggagaga aggaccgagg tgccttcctt gacgtagcgg cggatgatct cccggacgtc
      121 cagggcgttg aggacatcca ggcccgaggt cggctcgtcg aggatggcca ggaccggccg
      181 ggtcatcagg gcccgggcca ggagaagctt tcgggtcatg cccttgctgt aagtgccgac
      241 cttcgtcttc agccggttcc cgagctcggt gatggccgcg gcccgctcga cgaactcctt
      301 ttcctccccg gcgtcccggc cgtagaaacc ggccatgaag cggagataat ccattccctt
      361 catatccttg taggccccgg cttcttcggg cagatagctg attttctccc ggacgaaatc
      421 gggctccttg cggagatcgc ggtcgaggaa ggtgatctcg ccggcggtgg acgtcaagag
      481 ggtggcggcg atgcggaggg tcgtggtctt gccggccccg ttcgggccga tgagggcgaa
      541 gacctcgccc gcccggacct cgaaatcgat gccggccaga gccgcgtggg cgccgtagga
      601 ttttttcaat ccccggacgg tcaggacgtt ggacatggcg actccctgaa ataaaaataa
      661 ccttgatatt ctattccata cgaaagagaa agaaaaaagt ttctttccag cgccgccggc
      721 ccggcgcctc ttttcccctc gccgggcagg atcgaaatta aaagatcagg agcatcggag
      781 ggcgcgggga cggcccgccc gagtttcgaa aaattcggcg tgcagggccc ggacggccgg
      841 cagggcgtcc gctttccgca ccagaaagta agcggccgcg cccgaggctc ccgaggaaat
      901 catctcgacg ttgacgccgt tgccggcgac cgcggcgaag acgcgggcgg ccaggccccg
      961 ctccgacgag agtccttctc ccaccgccgc gaccagggcc aagttcgttt ccaagtccac
     1021 ccgggtgacc actccgcggc ggacggaccg caggatccgg cgggcccgcc gggcgtcctc
     1081 ctcggcgaca aggatgttga tcgccgtctg ggcggtgatg accgaaagga tgttgacgtt
     1141 ccgttcggcc aggctccggc cgatgtcccc gatgacgccc ggccggaatc ccacgcccgg
     1201 tccggagacg cgaaggacgg cgatccggtc gttgagcgtg acgcttttca ggacggtttc
     1261 ggccgttcct ccctcggccg cgatctccgt tcccggacgg tccggccggt caagatgccg
     1321 gatccaaatc gtcccgttct cccgggcgat cggctcgagg gcccggggat ggaggacctt
     1381 ggcgccgaag taggaaagct cggccgcctc ggtgaaggac aggcgggcga cgggcttggc
     1441 cccggaaacc cgccggggat ccgcggtcat gaagccgtcc acgtctttcc agatttccag
     1501 gagggagacg cggagggcgg cggcgacgac cgccgcgctg taatccgagc cgttgcggcc
     1561 gaaggagacg aggcgccccc cggcggccag gccgtaaaac ccggtgatga ccgggacgaa
     1621 gcggcccgcc tcgatcccga gggcgttttt tcgaacagcc cggcggaagg ccgcaaggtc
     1681 ggccgtcgcc ccctcgggga cgtcgtcggc cgccaggccg atctgctcgc tttccagagc
     1741 ccgggcgtcg gccccttcct ctcggaggat tccggccaga agcaaagccg cccagcgctc
     1801 gccgaagctc aagacatgag cccgggcata gggcgagatt tcgccgatca aggccgcgcc
     1861 ccggaggagc cgttcgagcc gggccaggcg atggccgatt tgggccgcga tctcggcccg
     1921 gatctcccgc gcggagagaa ggcctgaagc caggcggtcg ttctggagcc gaagggccgc
     1981 gatcgtccgg gcgatgccgg cttcgttttt gacggctcgg gccaggccgc gttccaactc
     2041 gtcggtaatc cccgaaacgg ccgagacgac gaccgccggc ggaacgggct cccgccggat
     2101 gacggccgcc gcccgccgga agccgtcggc gtcgcgaaga cacccgccgc cgaatttcat
     2161 cactttttga aatttcatcc gatcctcgct attcgtggag aagtccttgc cgggcggcca
     2221 gctcggcggt cagaagacag gcgccggcgg ccccgcggac ggtgttgtga accaggccga
     2281 aaaggtcgag gatgcggccc cgggagcgaa gccgcccggc cgtcacggcc attccccggg
     2341 ccctcgccgg agttcccgcc cagacgtcca aagccggctg gggccggttg tcttcggtcc
     2401 ggacgatgac cggccgctcc ggcgccgagg gcaggcccat ctcctgggga aggccgcgga
     2461 aggaggccag ggcttcggcc gcggcgtcct cgccgacggc tccctcgaat tcgacggaga
     2521 cgcttagaag gtgtccctcg cgaaccggga cgcggcagca gctcgcccgc accgggacgg
     2581 gccaaggaat gaaaccgtcc gcctcgaggg tcccgaagat gcgaccgggc tcgcgggcca
     2641 gcttctcctc ctcgccccgg atgaacggga cgcagttgcc ggcgatgtcc atcgccgcca
     2701 ggccgcgccg gccggcgccc gagatcgctt ggaacgtggt gacggcgacg gaacggattc
     2761 cgaacgggcg gagcggggcc aggagcaagg ccaggccggc cgtggagcaa ttgctgtcgg
     2821 cggcgatgaa tccgccgcgg ttggttttcc ggcgggcggc ccggacaaga tggtccgggt
     2881 tgatttccgg gacgacgacg gggacgtccg gatcgccgcg tcgggcggaa gcgttggtga
     2941 acacggcaag gccccggctc tggagggcgt cttccgcggc tccggccgcc gaggcgggga
     3001 gggcgctgaa gacgacccgg gctcccgagg cgatgaccgc ttcgaccgaa gccggttgaa
     3061 ccgttgtttt tccggcctgg gacggaagat ccccgccggc cgtccattca acggccgcgg
     3121 cgtacgggcg tccggtcgag cgctccgagc cggcgagaag ggcgatttcg aaatcgggat
     3181 ggccctcgag catccggacg aaatgctgcc ccaccaagcc ggtcgcgcct aacacggcga
     3241 cgggatattt gttcatgatg ttctcctttc gttattctcg aaatggccgg gaggctcacc
     3301 ggctgatccg ggcgggatcg acgcgcgttc ggacgatcgg cggaaggtcg ccccaaaccg
     3361 cgagcgtctt cccgacgacc gccagcagac cctccacgcc ctcgattcct cccaaggcgg
     3421 ccaaggtctc ccggagacgc gtttcgtcct cgtcggtgac ggcgtttccc aaagccgtgg
     3481 cgaccgcgtc ggccaggccg acgtccgccg aaaggacgac ggccgcgtcg gcccggccga
     3541 agctcagcga atgcccgacc gtccccgacg acgcgcagat tccgaaaatc cccggccggg
     3601 gctcgacccg gaaggcgatg tcgcggacgg gagacgggcc ggtgaagatc cccaccgtga
     3661 ccggacgctc gagcaggagg gcgatgtctc cgccgttgtc gacgagggcg tgggtcgctc
     3721 cggcttcggc catcgcccgc agggcggact cggccagcgc tccggccacc gcggccatcg
     3781 ggccgacccc ggcccgggac gcggcccggg ccatccggcg gacgatgggc ggagcctccg
     3841 gcggaacgga acgcggggcg agcgccgtcc ggaatccggg atcccgccgg atatagtcct
     3901 cgagccgtcc tcgttgtttt cgaatgtccg cctcggcgag ggggaggaaa ggctcggccg
     3961 cgacgatcgt cgcggccgtt tccttgatcc ggagcctttt gcggaacagg gcttcagcca
     4021 tggtgttccc ccggggcgct cgttttcggc cggcggattt cgagcggccg gacgacggat
     4081 tccgcaggga gagggacgac gggggcggtc aggaggaatg tcccggcctc gatccgccgc
     4141 tgaagctcgt cggcgatccg gcgggagagg acgaggctgg aaagggggag cgccggaacg
     4201 atccggcctc cgaggcgcac ccgtccggac cgcaactcgg cataggtgac ggaccggagg
     4261 gccggacggt cccgggaggg gaggccgaag tcgcggatcg tcacggcgat ctccgcgtcc
     4321 gagacggccg cgcggcgggc cgaatcggcg tcgaggatgg gaatcgggac gcccaggccg
     4381 atgcacaggg tgcagccgta gccggggaac agggccgccc ggaggaagtc cggcgacatg
     4441 cgcttcaaat cgccctggac ggccatcgtc ccgaagccgt tttgcggatc gtgctgggtt
     4501 cccgggccgg tgacgaatcc ttccgccccg ccgaggagaa ttctcgtccc caggccgatc
     4561 gtccggtagt ccgggtcgtt ggacagcggg gagagagagc ccgcgccggc atacgtcacg
     4621 ttgccgagac gcggccggag cgtccccatg taggtgtgaa gcgtccgcga cgaggagttg
     4681 gccgcggcgt tgtaccgctg atagccgttt cggggattgg agagcagggc cgtgttcagg
     4741 tcgttcagcg tcaggacggt caggaggcgc ttgcgcgggt agcaatcggt gcccggcgag
     4801 acggcttgaa ggatgacggg ccggccgcgg aggaagtcct cgatgacgtg ggcgccgccg
     4861 tagcggacgc cccgcgaagc ggagagctgg gtcgccccga ggtaggcgtc caccgcggcc
     4921 gcgccggcgt aggcctccac cccgttgagt cggacgcgtg tcattttgat cggcggctcg
     4981 gaatggccga agttgagcca gacgcccgag gagcacatcg gcccgaacgt ccccgtggtc
     5041 acgacgtcga cgttctcggc cgctttttcc ggaccgagct cgcggacgag gcggctcatg
     5101 tgatccgccc gaacgacctt cacggtcttc cgggcgattt tttcgttgat ctccgcaatg
     5161 gttttcggca tgacgacctc cctccgggaa tcaatggata gacaccggga acgaactcag
     5221 ggggcggggg attcccgaaa gccggcctcg gggagaggcc cgcttccggg cgtcacatga
     5281 tcatgccgac ggaaaagggc cggaggaaag acggacgggc ggggcggagc cgcgcggggg
     5341 cggccgggcg cttgacgtga tctcgatgat tcatcgttgt tccgctcgtt cgtaaccgcg
     5401 gttttataac acggcgggaa ccggatgtca atgaactgaa cagggtgaca caatgcttat
     5461 taaccctatt tcgatcggcc cctgcttcaa atcccgatct ggttgatcag gcctctcctt
     5521 gttcccgacc gtcaggaacg aggatttcgc catggattcc ttgccgacgg accattcccc
     5581 cggggagcgg atccgccctt cggtccaggc cgtcccggaa tccgatatcc ccaaggaagg
     5641 ctcgggggcc ttggaccttc ctcttttgtt cttgagtccg ccggggaagg tgggttaaat
     5701 taaggcaaac cagggaaagg agcgcgtccc catgagcaag cgtcggacca aacggatcgt
     5761 ccttgtcgtc ctttttctga tcttggccgc ggccgtcttc ctcgtcatta aaaaccagta
     5821 ttttcaaatg atgtccatcg gcaccggatc gaaggcgaag atcctgtgct cctcggtctt
     5881 tctttccggc cgcgacgcga ccgcggtcga ggccgaggat ttgggctttc atccgcttta
     5941 caagatcttc aagacgaagg tcgactatga acagaaacgg gtgagctgtt ctctgctggg
     6001 gacgggcttg ttcaaggcgg aagcggtctt ccgcgagggg atcggggccg tgcttctggc
     6061 cggcggagcg ttggaggaag ttgtccgggc ttggcccaaa ccggagatcg acgcggccgt
     6121 tgaagagacc gatcccgagt ccgtcgactg gccgatgggc gaccggctga tcctcgtccc
     6181 gccggggacg acgatcgacg cggcccggat ccgggccgcg ggcgaccggc ttttttcaga
     6241 ggccgatccg aatcatcctc tccggacgcg ggccttgatc gtcctccacg acggccgctt
     6301 ggtttatgaa cgctacgggc cggggatcac caagaatacc cgtctcctga gctggtcgac
     6361 ggtcaagagc gtcacctgcg tcctggccgg catcctcgtc ggccgggggc ggctcgatct
     6421 cgacctccct gtcccggtcg aggcttgggc cggagccggg gactcgcgcc ggaccatcac
     6481 tctcgaccag ctgctccgga tgagctccgg gctggaatgg ttcgaagctt acgccgaccg
     6541 ccccgtctcg gacgtcaacg acatgctctt tcgaaagccc gacatggccg cgttcgccgc
     6601 ctccaagcgc cgggcggtcc ccgccggcgc ggcctggaac tactcctccg ggacggccaa
     6661 catcgtccag ggaatcctgc ggaagacgct cggttcggac gaggagtact ggaatttccc
     6721 ccgccgggcc ctgttagacc ggatcggaat gcggagcgcg gtgatggagc gggacgcgtc
     6781 cgggacgttc gtcggatcgt cgtatctcta cgccacggcc agggatttcg cccggttcgg
     6841 cctgctctgc ctcaacgacg gcgtctggct gggcgagcgg atccttccgg aagggtggat
     6901 ggcttatgcg acggcgcccg ctccggccgc gccccagggg cgatacgggg cgttcttctg
     6961 gctgaaccgg ggcgatccgg gaaaaccgga gacgcgcccg tatcctctgt tgccggaaga
     7021 cgcgtatttc gctctcggct accagggcca gacgatcgcc gttttgccgt cgcaaaagct
     7081 cgtggtcgtc cgcctcggga tgacctacga cgacgactgg ggcggaatga acgtcttttt
     7141 gcccgaaatc gccgcggccg tcaagtgagc gggggccggc cccggattat ttctggacgc
     7201 tctccggagc cggaggcaga ggcaccagcc cggccatctc gccgatgacg ttgatcagct
     7261 gggtatcggc cgggacgacg atcttggcat tgtgggccag cgctttttcg gcgacttcca
     7321 tccgtttgag gacctgggcg ttgccgatga aataggcctg ggccgcctcg ttgacgagct
     7381 tgatggcctg ggcctcgccc tcggccgcga gaatccgggc ctgcttgacg ccgtcggctc
     7441 tcttgatttc agcccggcgg gcgccgtcgg ccatggtttc ggtcgcggtg gcgaagtcga
     7501 cggccgcgat cttctcgttc tcggccttga cgaccttgtt catcgtttct tggacgtcct
     7561 tgggcgggtc gatttccttg agctcggtcc gaacgatctc gatcccccag ctgccggttt
     7621 cctcgcggag ggttttctgg agctcggagt tgatcttgcc ccgttcgctg ttggccgact
     7681 tgagggtcaa ggtgccgatg atgttgcgga gggtggtccg ggccaggttg acgatctgcc
     7741 actggtaatt gttgacgttg tactggctgt ttttgacgct ctgctcgtcg tttttcacct
     7801 tgaagtacac ttgggcgtcg acccgggcgt tgagattgtc gttggtgatg atctcctggg
     7861 gctgggcgtc caccatcacc tcggtcacgt tgacctggta gagacggtcg atcaccggaa
     7921 tgatccagtt gaatcccgga ttggcgaacc gcttatatct tcccaggcgc tcgaccagcc
     7981 cccggtgggt cgggcggacg atgcggatgc cgatgagaaa gaaaaagacg atgatcgccg
     8041 cggcgagaat gatgatttcc gtttgtttca tgggatcctc cttcccggtg aatgcctttt
     8101 ttcattatag cccgggtttc ccggagagac aagaaacggc cggccgcccg gccgggaccc
     8161 aatccggcgc ggggtcggcg cccttcccga tgcccgatca atcggtacct gtttcaaatc
     8221 ggtacctgtt taaccatttc ctgatttttt ggttcttttc catccgaaag tcggcaagtc
     8281 gggcccgtga aaaggagcgg ccggccggcg ctttggccca tcgcctaatt tgctccctcg
     8341 gcggcgggag ggaggggcgg aagacgaaag gccgggagga tcaggcgagg cgggatcagg
     8401 gggaaatccg gaaatggata accaggttcg gattgaggtt cggatcggcc gggcggggaa
     8461 atgtggaaat ggttaaacag gtaccgattt gtttgtgata tcatcgggcc gtgaagtccg
     8521 gaaaaacggc cgcctatttc ctgatcgccg ccgcggcttt cctgtttcca tcctcctgcg
     8581 tgaaccggcg gcgggaggcg ccgccgccgt cgtcctcctc gctctcgtca tcctcctcat
     8641 tctcgtccgc cgcggcccgt aactatgccc tgatcgagcc gggtgaatcc atcgattcca
     8701 tcatccgcaa agccgccgcc gtcgttccct cgccccgcca atgggcttgg caggcccgcg
     8761 agttcatggg cttcatccat ttcgggatca acaccttcac gggccgggaa tggggcgacg
     8821 ggacggagga tccggccttg ttcaatcccg cggacttcga tgcccgccaa tgggtccggg
     8881 tcttcaagca ggccggaatg aaaacgctga tcctcacggc caagcaccac gacggattct
     8941 gcctctggcc tacggccgaa accgcccacg gcgtgaagag cggtccctgg cgcggggggc
     9001 ggggcgacgt cgtgggcgaa gtcgccgcgg cctgccggga ggccggcctc ggcttcggat
     9061 tctacttgtc cccctgggac cgccatgagc cctcctacgg cgattcgccc cgctacaacg
     9121 cttttttcct ggcccagctg cgggagcttc tgaccggcta cggccccatc gccgaggtct
     9181 ggttcgacgg cgcgccagga gacggcccgg agcggaagaa tcaaatctat gactggcccg
     9241 cgtattaccg gacgatccgc gagctccagc cggaggccgt catcgcgatg atggcccccg
     9301 acgcccgctg ggtcgggacc gaatccggct acgggcggga aacggagtgg agcgccatcc
     9361 cggtcgacgt ccggaatccg gaatcgctcg ccgccgcctc cggtctcttt cccctggacg
     9421 acgttttcgt cccccgcgac ctgacggcgg ccgatctcgg gagccgggaa aagctggccg
     9481 gggcccgggt cttggcctgg tatccggccg aaaccgacgt ctccatccgg cccggctggt
     9541 tctatcacgc ctcccaggac ggggacgtca aatcacccga gaagctcctc gatatctacg
     9601 aaagctcggt cggccgaaac ggcgtcctcc tcctcaacgt cccgcccgac cgccgcggcc
     9661 tcatccacga gaacgacgaa gaggccctcc gggagctgcg ccgctccctc gacgcggcgt
     9721 acgggaccaa cctcgccgcc ggggccgccg tccgggcgtc cgcgtccgcc accgggcacg
     9781 gcccggagac gatgttggac gacgatttga acaccttctg gacgccgccc ggagagggcg
     9841 gacgggcgga aatcacgatc gatcttccgg gtccttcgga gttcgaccgt ttcgttcttc
     9901 gggagcatat ccggtccggc cagcggatcg aatcgtacgt cctggaagcc ggggagggga
     9961 acgcctggcg gcccgtggcc cggggaacga ccgtcggagc catgaggatc ctgcgcttcc
    10021 cggcggtttc cgcccgccgc ctccgtctcg tcatcgaaca gtcccgggcc gctccggctc
    10081 tcgcctcgct cggcctccac cgcgtccgga cggagagcgg gccgcgatga acaacgtcga
    10141 aatcaaggcc cgggtccggg atctcgacgc gttcgccgcc cggctcaagg cgctccggcc
    10201 gtcgaagccg cgcgtccttg tccaggagga cgtctttttc aagatcccga ggggccgcct
    10261 caagctccgg atcctcggcc cccggaccgg ggaattgatt tactacgagc gtccggacgc
    10321 ggccgggccg aagctctcga gcttcgagat cgcccggacc tccgatccgg ccgggctccg
    10381 ggccgtcctg gcggcggcct tcggcgtccg cggcgtggtc cggaaaatcc ggctggccgc
    10441 ccgggccggc caaacgcgca tccacttgga tgccgtctcc ggattgggct tgttcgccga
    10501 actggaggtc gtcctccgcc ggggccaggc ggccgccgac ggagcggccg tcgcccgcgc
    10561 cttgatgaaa aaattgaaaa tccggccggc cgacctggtc tccggcgcgt acctggatct
    10621 tttggaaaaa gcgtccgggc cggccgtcag tccttctgcc aagacggctt gtgatcgtaa
    10681 acccagcggt aatactccgt ccgcttgagg ccggaagccg cttcttcatc caggatgacg
    10741 atcgcccggg gatgcatctg aagaatcgag gccggaacca tggccgagac cgggccttcc
    10801 acggcctggg cgatcgcggc cgctttcttc cgaccgaacg ccaggaggag acaggtccgg
    10861 gcgtccatga tggtgcccaa gcccatggtg atgacgtggt ggggaacgtt gtcctccccg
    10921 ccgaaggcgg cggcgttgtc cttgcgcgtc cggtcggtca aggttttgat ccgcgtccgg
    10981 gaggacaacg acgaggacgg ctcgttgaag ccgaggtggc cgtcggagcc gatgcccagc
    11041 agctggaggt cgatgccgcc ggccgcccgg aggcgctcct cgtattcccg gcaggtccgg
    11101 ggaatgtcct cggacattcc gtcggggagg aaaacccggg ccggatcgat gttgattttg
    11161 gaaaacaggc tcgtccgcat gcaggcgtga aaggacgccg ggtgggaggg ctccaggccg
    11221 aggtattcat cgaggttgaa cgtcgtcacc cggctgaagt cgaggccttc cgtccggtga
    11281 agctccgaca gccggtcgta cgtttcgagc ggcgtttggc cggtcgccag tccgaggacg
    11341 gcgtccggct tcgcccggac caaggcggcg attttccggg cggctaaacg ggaggcggcg
    11401 tggctgtcgg gtttgatgat gacttccatg aagattctct ctttccgatt ataaccggtt
    11461 taccgggaga agcgaagccg cggtccggcg gtccgtccgc actctttatc gatccgcgga
    11521 ccgagcttcg gcccggactt cgcggaggag attccaggcc gcgtgatcga ggagcaacgt
    11581 gtcggtggag acgcacagca gggtgaatcc ttcggcggcc gccgcccggg ccgcggcggc
    11641 gtcggggctg aagattccgg aggggaggcc tcggccggcg caggcctcgc ggatccgggc
    11701 aatcgccccg cggacgaggg gggcgtccgt tcggcccggg acgcccaggc tggccgagag
    11761 gtcgaacggc ccgacgaaga cggcgtcgag cccctcgacc gagaggatgt cggagatgtt
    11821 ttcggccgcc tcgatatgct cggcttgggc gacaaggacg attttatcgt tggcttcgtt
    11881 cagggcttgg gcgacccgcg ctccgtagcc ctgggcccgg ccgaggccga tgctccggct
    11941 tccctgggga gcgtagcggg cggccttgac ggccgcccgg gcctcggccg cttggttgag
    12001 gtgggggaaa atcaatccgt cggcccccgt gtcgagcgcc ttggcgatcc aggccgggtc
    12061 gttgaccgcg acgcggacga ggcaggcgga gcggccggag acggcctgga ccatccgctg
    12121 gacgtcggaa aaaccgagca gcccgtgttc catgtcgaga aagagccagt cgaaaccggc
    12181 cgaagcgcag gtctcggcga tttccggaga gggaagcgag aggaggattc cggtcaagag
    12241 atcccctttt ttcaaacggt ttttaaaggc cggagcgggc ggaatggtca tggcgcgcct
    12301 ccttgacgga cattatagcc ctttttgact ccccccggcg cctgtgctat aagcggttca
    12361 gaaaggctct cacgatctta tggccgatat cgccaggggc gtccgctcgt tcgccgtcgg
    12421 caccggactc agccgcatcc tcggccttgt ccgcgaacag gtcttcgcct ttctcttcgg
    12481 cgtcggggcg gcttcggacg ccttcaacgc cgcgttccga atccccaatc ttttccgcga
    12541 cctgttcgcc gaaaacgcgc tgtccaacgc gttcgtcccg gtcctgaccg ccgcccgggc
    12601 caagggccgg gaggaggaga accgctttgc ctccaacatc ctcaacactc ttctcctgac
    12661 ggtcggcctg atcgtcgtcc tggggatgat tttctcgccg gcgctggccc gcgtcgtcgg
    12721 tctcggcttc ggcgcgatcc ctggaaagac ggccctcacg gcccgcttga cgttcctcat
    12781 gtttccgttt ctcctgttca tcgttctggc cgcttgggcg atgagctatc tcaacacgga
    12841 gaacgagttt ttcatcccgg ccgtcgctcc ggcgttcttc aacctctttt cgatcatcgt
    12901 cccggtcgtt cttttcggct ggttcgtgaa tcgcggagag gatccgatcc tgggcatggc
    12961 cttcggcgtc acggccggcg gattcgtcca attcctcgtc caggtcccct cgctttcccg
    13021 ccggggttac gcctaccggc tccatctcaa tttccgggac gcggccttcc gcaaggcgat
    13081 ggcgcttttc gtcccggtgg ccatcggcct ctcggcctcc cggatcaacg tcctcgtcaa
    13141 caccatgctg atctcggtca tcccgggggg catcagctgg ttgaattacg cttaccgcgt
    13201 ttttcacctg ccgctgggga tgctgggcgt gtcggtcggg caggtgtccc tgccgctctt
    13261 ttcccggctg gtcaacgaga ggcggttcga cgatctccgc cggacgctgg acgattccct
    13321 ccgcctcgtt ttgttcctga cggtgccgac gtcggtcttg atcgccgtcc tggcccgccc
    13381 catcatcgcc gtgatctacc agcacggcct cttcaccgcg gccgacacgc gggccacggc
    13441 cgttatgctg gttctgtacc tgatcggcgc gccgttcgtc tccgccctcc gcaacctcac
    13501 ttcggttttt tacgcctgcc gggacgcccg gctcccgatg atcgccagct tcatctcggt
    13561 cgggaccaat atcgctttca acctggcgct catgggcgtc ttgggacacc gggcgtttcc
    13621 cctttcggcc tccatcgcgg cggttgtcaa cttcgcgatt ctcctcgccc ttctcccgag
    13681 aaagatcggc tctttaccgg ccggtccgct ggccgggttc gcggtccggc tcgtcctggc
    13741 ctcggccgcg ggcggcgggg cggcctgggt cggggcggcg gccgccgccc ggatcgccgg
    13801 aacgtcgttc tggagcgggc tcggggccgt cgctgtctgc gggacggccg gattggggat
    13861 cttttacgcg gtttgccgga ttctcggact ggacgaggcc cgcgatttcg cccggcggct
    13921 cctccgccgc ggccggagcg aggacgccgc tccgccggcc ggaaccgatc cggtctaatc
    13981 gggttatcaa cgacggttcg cccggccttc cgggcgcgat ctcccggttt ccgaaataag
    14041 ccgtccgccg cggccggcgg gggcggttcc ttgggtcgat ccctctcagt cgagaagccc
    14101 gagaatttcc gacggccggg agacaaggac ttgagccccg ttcttccgga gctcgtcggc
    14161 cgtccggaat ccccagagca cgccgacggc gaacatcttc gccgcggcgg ccgtgcgcat
    14221 gtcggtgttc gtgtctccga cgtacaggat ctccccgggc ggaatatgga aacgccgggc
    14281 gatatccagg gctccggccg gatcgggctt gaccggaatc tcctcgcggg cgccgcagac
    14341 gaagcggaag ggaacgcccg ggaaattctt ttccacgact tcccgggtcg aatcgtgggg
    14401 cttgttggag agaacggcca gcgggatttt ccgttcggcc aacgccgcga gaagctccgg
    14461 gatgccggga tacggccggg ttttaacggc gttgcggagg cggtattcgg cccgcatgcc
    14521 gtcgtagagc ttccggaagg cctcgggatc ctcggcccgg gaggaaggca gggcccggcg
    14581 gatgagcgtc gccaggccgt cgccgaccag gattttgcat tcctccacgg ttttcgccgg
    14641 gaaacccagc cgggcgaggg cggcgttcat cgaatcggtt aaatcctcga tcgtgtcgag
    14701 gagcgtcccg tccagatcga aaatgacggc ttgaaatctc atgattctcc gggaggcgaa
    14761 gacctgttcc cgcggccggc cttattcacg aatccatcgg acggctcggc ttcgtcgcga
    14821 agggggaaca cgcctcatgg cgatcttata aaaaagagaa gaaattatac aatttcaaga
    14881 tcttaatgat caagacaaac cgaaggaatg agaaagacgg tccggagaaa tcgaacgcct
    14941 cccatcccgg ccgacgcgcc tccctttttt tcgggccgaa acggcgaaag gagccttgtc
    15001 cctatccaag aacattgact agtttgatcg gcgtcggagt ctttcaaggg cgtccccgag
    15061 cttcccgcct cgttttcttt gcgctcggat ccagatcctt gtccctgggc gaccgggggc
    15121 ggcggggacg gtcctcccat cccgagcctt atcccggcct ggggaacgag ataaaaaaag
    15181 catccgggag aaaagagggt aaccgtttca gcgggagggg atgggacgcc gcctcccgtc
    15241 aagccgctgt caaccccatc caaaagagcc tcgtctcgcc tccggcctcc ttgggtaaag
    15301 agaagagggg aaaatgattt acggcgcgat tcgatccttt gacagaggcg aattccgtct
    15361 gctacgatga aaacgtcaag gaaaaggggg gaggaggcgc cgatgctggc catcaagaaa
    15421 atcctttgcc cggtggattt cagcgagccg tcgcttttgg gcctgtcgta ttccgcggag
    15481 ctggcccggt tgttcgaggc ggacatcgag gtcatctatg tcctgccgat tttacctccc
    15541 cagccgatcg acccccagtt ctccttccag gtccccgagt acgaaattct tcttcaccgg
    15601 gacgccgaaa cccgcctggc cgctctcgtc aaggaaaaga ttcccgaggg gatcaaggcc
    15661 gttccggtcg tcggacacgg cagcgccggc aaggagatcg tgaagcgggc cgaggagacg
    15721 aaagccgacc ttatcgtgat ctccagccac ggccacggcg gttggcatca cttggtcatg
    15781 ggctcggtcg ccgaacgggt gatccgccac tcgccctgcc cggttttcgt cgttcgggaa
    15841 gcgaggaagt aagaaaggga aaggactgaa aggagcgccg cgggggagac ccggtctcgg
    15901 acggaggcgg cggggatgcg gagcgcgttt ccgcgggaca agaggtgatc cgcacctcgg
    15961 cttttgggtc ccgggggcgc ccggccgggg cgaaaggcgg gcgtttccgc ccgaaaccgt
    16021 ccgttttcgg ttgacctgcc cccccctttt tgctatcttc tctactccac ggcgctatcg
    16081 tctaggggtt aggacggatg gttctcagcc atcaaaccgg ggttcgaatc cccgtagcgc
    16141 tactttttct ctttcctcgc ccgcttttaa attccgcctc ctcatccgcg ccctccgagc
    16201 gtttttatgc cgtagcgttt ttgcggggct ccgcccaggg cgtccagggg actcgatccc
    16261 catccgccca tgacgtagac cgagggatag cgccgctccg gattctggaa atagcccggc
    16321 ggcaggatga cggccgcccg gatcgattgc gctttcccgt agaaccgggt cagccgcggg
    16381 ctgggaacgt tcgcctcctt gatgaattcg ctttccttga attccggcgc cgggccggcg
    16441 ggaacgtgaa tcagcaggtc gagcggccgg ctcatcctcc ctttctcgat gaaaacgacg
    16501 ttcttgacgc tccgggcgtt gcccggggcc tgaagcgtcc gggccatgcg gttggccttg
    16561 agggacgctt ggacgccgta ccagccgtcc agggcggaga ggtcgccgtg ccagcagacg
    16621 gcgtcccggc cttcgaggat gatggcttcg ccgggtttcc atccgcggac atcccaggcg
    16681 aaagtcggct gagggtcgag gaggtcggga ttatcgatgg gggctgaagg gtcccggtga
    16741 aacccgatga taatccgccc ggacacgggc gacgggctca cctccgcggt gaacgaaacg
    16801 cggagacgaa gctcttggag cccggaggtc tccggagtct cttgttccgc tgcggccgac
    16861 ggaaaagctc cggcccaaag acaggcgaat aaaaaaatca gaaagccgat gatcaggaac
    16921 gttcttttca caatattttc tccttgaccg aggctgaaag atcgcgggaa ggagttctca
    16981 tcatccggcc gcgaagccgg cgtcaaaaac gaggagatcc gcgcgacgtc cgccgcgcgg
    17041 atccgcctcg ctcgaaatcc tccggatgat cttgacgccc atgaatgaca actccttctt
    17101 ccactacgca ggaaaaagga ttcaggttgc cgtctcttat gagaaggggg cgggcggacc
    17161 gaaaaaacgc ctggccgcgc gggccttttt gagggagaag gcgcaaggaa aagctcccgc
    17221 ccccccggcg gatgatccgg agggacggcc aggacacggg aggaatcgac ggccgtctac
    17281 ccggccgctg gtttctcccc gggagtcggg cgtataatat ccccggacgc ggttcagccc
    17341 atgagcggaa cggaaaacgg ctctccccgg ccgaaaaaaa agcatgtctt cggtaaggcg
    17401 gccgccgcgg gcgcggcctt gctgatcgtt ttcgccggga tggtttggct gaccctgccg
    17461 gacgtggcgg atctgaagac ggagaacccc aagtcgaccg ccctcatcga gcgccggaag
    17521 gccgacgccc gggcccaagg gaaaaccctc gtcgtccgga tccattggat cgccttcgac
    17581 cggattcccc cgctcctgaa aaaaagcgtc ctcgtcagcg aggacgcgtc gttttacgac
    17641 catcagggcg ttgatttcgc cgagctcaaa gcctctttca agaaggacct caaggaaggc
    17701 cgcttcgtcc gcggcgggag cacgatcacc cagcagctgg ctaagaatct ctacttgtcc
    17761 ccggccaagt ccccggtccg aaagctccgg gaattcctga tcgcccaacg cttggagagg
    17821 acgctgccca agaaccggat ctttcacctc tatttgaacg tcatcgagtg ggggccgggg
    17881 attttcggag cggcggccgc ggcccggcac tggtttggaa aggacgtcgc cgccttgact
    17941 ctcgaggaaa tggtccggtt gacggccgtc atcccgcggc cgctgcggtc cgatccgcgg
    18001 aaaaacgacc gctggatgcg gttcaagggc cggtggatcg ccgatacctt gagaaccgtc
    18061 ggggcgatcg ggggggatga ccatcaagct ctcgtccggg cgttcgagga atctcgatcc
    18121 cccctccctt gagaaccaag ccgctcgcca ggggcttccg ccgccgcagg atccgtttcc
    18181 gtcggggccg gctcccgccg gctgtcgaag cttcgaaacg gtcaccggga ggccgaacaa
    18241 aggaagaatc caggggcgcg tccccgggat tcgcttcgcc ttgacagcgg ctccaatcgg
    18301 actagaatga ggacttatcc cgcctaattc acgagccggg cgccgggagg aattcacggg
    18361 aagcgcggat gtcgcctcgg ccgattcaga ggatgttttc gcgaatgagg cggggtgatt
    18421 caaggaggcc ccatgggcgg attttttgga gtcgcgagcc gggccgagaa catttccgac
    18481 ctgttttacg ggaccgatta ccattcccac ctcggaacga agcggggcgg gctcgccgtc
    18541 gtccggcccg agggcttggt caagtcgatc cacaacatcg aaaacaccca gttccgggcg
    18601 aagttcgatc ccgatctcgg cggcttgacg gcgaacctcg gcatcggcgt catcagcgat
    18661 tccgacaatc agccgatcgt gattcattcc catctgggac gattcgccgt cgtcaccgtg
    18721 gccgcgatca aaaacatgga cgcgctcgtc cgccgggttt tccgcgagcg gcgcattcac
    18781 ttcgccgaat ccatgggcgg gggggtcaat ccgacggaac tggcggccgc gctgatcaac
    18841 caggaggatt cgttcgaagc cggaatccgc aacctccagg actcgatcga gggctcgtgc
    18901 tcggttcttg tcctgacgga aaagggcatc tacgccggcc gggataaata cgggcggacg
    18961 ccggtcattc tcggcgccaa gcccggggct tgggccgtga ccctggaaac gtgcgctttc
    19021 ccgaacctcg gtttcgagac ggtccgcgag ctcggacccg gagagatcgt ttttataacg
    19081 cccgaagggg cggaaacccg gcagaagccc ggcggcctcc tccagatctg cgcttttctc
    19141 tgggtgtatt acggctttcc ggcgtccaca tatgaaggga tcaacgtcga ggcggcccgc
    19201 aaccgctgcg gggaagccct tgcccgccgc cacccggccg ccgtcgattt cgccgcgggg
    19261 atccccgact cgggcgtcgg ccacggcatc ggctacgcgg ccgcggccgg cattcctttc
    19321 aagcgcccct tcgtcaagta cacgccgacg tgggcccgga gcttcatgcc tcagaaccag
    19381 gacgtccgcg acctggtcgc ccggatgaag ctgatcccga tcaaggaact catccagggg
    19441 aagaggttcc tcttctgcga ggactccatc gtccggggaa cccagctgaa ggacaccgtc
    19501 cagcgccttt acgacacggg ggccatggaa atccacatgc ggccggcctg ccccccgctc
    19561 atccacggct gcaaattcct caatttttcc gtctcccgtt cggagatgga cctggccgcc
    19621 cggcgggcga tccgggaaat cgagggagag aagccggtgg acctttcgct ttacgccgac
    19681 gagcggtcgc cccaatacga ggccatggtg gagcgcatcc gccgccgtct caacctcacc
    19741 tccctgcggt ttcaacgcct ggaggacttg atttgggcga tcgggctgcc caaagaccgg
    19801 ctgtgcacct attgctggga cggcgtcgag gtcaaaagcc cgtccctgcc gttcgcctga
    19861 tccggggaaa acgccggctg cgcttcggct tattccacgg gtccgcagtc aggggacggg
    19921 aagataaata atcccttgaa aatccgcgga agaagaccgg ggaggggaaa agaggatggc
    19981 tgggaagcta ggattcgaac ctagatacca tgatccagag tcatgggtcc tgccgttgga
    20041 cgacttccca accggaaggg gttgaatata gcggattttc ctcctctttt caagcccttc
    20101 cgggcggcgg gggggttgtg ataatatgcc ctccgcatca tgatccggaa acagtggttc
    20161 cgcctctttt tcgtcctcgg cctgaccggc gtcctgcttt atgtcttctt gcggggcgtg
    20221 gactggagcg aggccttccg atacctcaag gaacacgtcc gctgggggat gtttctcttg
    20281 gttattcccc tcagccagat ccacatcctc acccgggcct ggcggtggaa gattctgatg
    20341 gtccacgaaa aaaaggacgt tcggctgtac aacctggccg cggccaacgc cgtcggcttc
    20401 accgtcagct atcttttccc cggacggatc ggagagctgg tcaagcccct ttacctcgcc
    20461 cgccaggaga aatgccgctc cgggttcgcc atcgggacat gcgtcgtcga gcggatcttc
    20521 gatgtc
//
LOCUS       DAOOXH010000038        20511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_2459_length_20511_cov_6.888541, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000038 DAOOXH010000000
VERSION     DAOOXH010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 20511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20511
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2459_length_20511_cov_6.888541"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PKV64_06045"
     CDS             <1..484
                     /locus_tag="PKV64_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HPN16676.1"
                     /translation="ILDVFLIASGFILRVVAGGVVLRVSLSSWLIICTSLLALFIGFG
                     KRRHEIVFLEEDASLHRSILREYSPLLLDQMISVVTASTVIAYSLYTVSEETVRKFGT
                     SNLIFTMPFVLYGLFRYLYLVHQRGQGGNPEEMIFRDKPLAATIVLWIVSVVMILYVR
                     "
     gene            complement(462..1232)
                     /gene="recO"
                     /locus_tag="PKV64_06050"
     CDS             complement(462..1232)
                     /gene="recO"
                     /locus_tag="PKV64_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPN16677.1"
                     /translation="MPRDQAEAIVLRTFPLADQDKIVVFLTAERGVLRGVAKGARKFA
                     NRFGSSLEPLSHVTVFFYEKERSDLVTINSCDLRESFFDVFSDPAAGFALSYMAELSE
                     EFVPVRARENLAFRLFLSILRAVRDKVDVQILTRYFEAWLLRLNGILPDIRRCRLCRR
                     AIEDPAPAWLSPRRDGVFCDACAPSRKEEIRESLGRFLSWVRDNPPSACASLPFSAGE
                     IRSFAGALQAMIVFHLEREPKSRRWLQDENGLSGRKES"
     gene            complement(1239..2594)
                     /locus_tag="PKV64_06055"
     CDS             complement(1239..2594)
                     /locus_tag="PKV64_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPN16678.1"
                     /translation="MSQERVLVIDDESAIRSSLRGILEDEGYVVRTAASGEEGLAALS
                     KESFGLVILDIWLPEMNGLDVLAEIQRHEDRPAVVVISGHGTVETAVKAVKMGAFDFL
                     EKPLSLEKVVLTVRNSLLRARLEEENVRLRERLAGRNCLVGKSPALQNLRAKIRAAAP
                     TDGRILISGENGTGKELAARLIHQRSPRRDRRFVEVNCAALPETLIDAELFGCLKGHG
                     PDPAKEKKGKIVQAGGGTLFLEGIETLTLRTQAALNLALAKGSFEPLGGSEPVPFDAR
                     VIASTKASLGDLVRQGRFNEDLYFKINVIPLTLPPLRERLEDIPVLIAYFLRIYCREY
                     GRMPKSIHPEALQAFVNYSWPGNVAELMNVLERFVILVEDQVIGPGHLNLLVETREQG
                     PMPASRPSLAEAVRRAEREAVHQALRRHQWDEARASAELQITPENLRAKIRDLRITLL
                     P"
     gene            complement(2591..4768)
                     /locus_tag="PKV64_06060"
     CDS             complement(2591..4768)
                     /locus_tag="PKV64_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN16679.1"
                     /translation="MDENQGRRQKKGTRVVVSVILLLVILFFVIDVFLRGSAEFSPGK
                     VTSILLAALQFIVLLLALILFFVLGRYLVKLYLERKQKVVGSHFKTKLVIFFTALSFI
                     PTLLLFIFTSRIINNSIEQWFRLDITRLVKDTRAVADGFYATTSDETFHFAQQLAREI
                     QRSELADPARRPRLEDFVKGKLNEYRLDEISLFLGGEELFSLLNPNLPLQDYRDLNAD
                     EVRKAHLGLDLNDITPMGAGEFVRRGVSFIDPKMGAVLVTAGKFLPQNLTQKMNSIMA
                     LDERYRMRGTQRDLTKTLYTLTLIFVTLIIVFTAIWIGFHIAKSITVPIEKLAYATKE
                     VSRGNLSIKIEDPASDEIGTLIDSFNQMTADLRKGEAEVAAKTAEVAARQRTIEIVLN
                     AISTGVIALDARGIVATINPAAREMLDLPEKNATGRPYRDLLASERYRALRAAIETAF
                     VNRARISDREIPMTMGVQTKTLSLNLTPLRKPEEDFSGLIVAVDDLTQLITAQKIAAW
                     KEVAQRVAHEIKNPLTPIQLNAERIIRNIHKGDPSGGEVVAEGARVIIQEAQTIKALV
                     DEFSDFARLPKIHLQPASLGDILRQVANLFRGIYADIVFEIRIAPDVPAEIQLDAEQI
                     KRVFINLIDNAIDAMNKKGTITLQADYDRDSRRVRATVTDTGPGISVEDPEKIFLPTF
                     STKKKGRGLGLAIVSQIIREHDGLIRAENNRPMGAKFTIELPA"
     gene            complement(4774..5568)
                     /locus_tag="PKV64_06065"
     CDS             complement(4774..5568)
                     /locus_tag="PKV64_06065"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HPN16680.1"
                     /translation="MATLGQELRTEREKHSLSLKDISDRTKVGLRYLVALEEDRWEEM
                     PQKFFIRSIIKAHAQAMGVDPEPFLARYEEQEKARLESPGNEGESPKRKKRGAEAGGT
                     RRILVPALAVLLVLGAAAAGYLLFLKPGEKSAPPVSSTEIPAPQTEAALPEPAAAKEP
                     EPVPAETGLRLELRFNADSWMHISADGVVVLDGIKPAGSTASLRAETEFILQTGNAGG
                     FDFTLNGRPGRPLGGSGVVLTDIRITRENSTTFLREENPPAGGAVR"
     assembly_gap    5792..5891
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5908..6807)
                     /locus_tag="PKV64_06070"
     CDS             complement(5908..6807)
                     /locus_tag="PKV64_06070"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN16681.1"
                     /translation="MNGRKIGRAFRDAGGHLRRGKTRHAVSFAVLVLSFLMAGLFLSL
                     SNNLRSRARILSRDVAVVVYLSPSATAAERRTIEDRVRLSPLVASVRTVTPEEAGDRF
                     LKEFPDLRDVLENLGRNPLPASIEAILRDPAGPDAPIDRMIDGLRRLPGVEDVQFNRR
                     WSDRVRALGRLSDAVGVVVGGLLILMSIAVVSGAVRLNILSRHEEISILHLVGATNGY
                     IRGPFLLEGLILGVAGGTVAAGLVLLAARLFPLYLGHSLGALQDLIGFEPLSLLQAAG
                     LVAGGGLAGLLGSLAALGRVFRS"
     gene            complement(6804..7472)
                     /gene="ftsE"
                     /locus_tag="PKV64_06075"
     CDS             complement(6804..7472)
                     /gene="ftsE"
                     /locus_tag="PKV64_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HPN16682.1"
                     /translation="MIEFSHVWKQYHKPDWALSDVSFAVAAGEMVFITGPSGSGKSTI
                     LRLMTRECPPTDGRILIEGMDIRRIPSAKIYILRRAMGVVFQDFRLLAERRVFDNVAV
                     PLRVLGLPSKDVKRKVFLALRMVGLHHRIWNTPAGLSYGEQQRVAIARAVVNNPRLLL
                     ADEPTGNLDPDLASEIIALFREINRQGTTVVICTHDREIVRKFAGRVLALREGRMRER
                     GTAP"
     gene            complement(7504..7683)
                     /locus_tag="PKV64_06080"
     CDS             complement(7504..7683)
                     /locus_tag="PKV64_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16683.1"
                     /translation="MIFRSHLLSMILYAALVAVVLAFLRRDEAKSRLKYGLIMFGGMA
                     GGAVLFGWLMYLFTL"
     gene            complement(7680..8048)
                     /locus_tag="PKV64_06085"
     CDS             complement(7680..8048)
                     /locus_tag="PKV64_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16684.1"
                     /translation="MKIKAVVLMVLGWAVPGLGHFLQRKYLRGAVFFGCLAAMTALGL
                     ALGGRIYSFQTENPLTILAFFADIGNGLLYLLSRVLPVGIGDLKLATFEFGTAYLAGA
                     GLLNFLVALDAYDIAAGTKS"
     gene            8191..9015
                     /gene="mutM"
                     /locus_tag="PKV64_06090"
     CDS             8191..9015
                     /gene="mutM"
                     /locus_tag="PKV64_06090"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HPN16685.1"
                     /translation="MPELPEVETIAAALRSAIVGREVAAARCFLPKLLRATEPRDLDA
                     LAGARILAVRRRGKILILEAPPRALLFHLKMTGRFDWENAGRPLSKHTHFVLSFAGSD
                     GELRFRDVRKFGFLRCLPRGEVEACDEIRRLGPEPLDIGPEEFAARLAARRGRLKSVL
                     LDQRVLAGVGNIYADEALHAARLHPLTAAGRLRPDELRRLRASLRRVLAAAIAAGGSS
                     IRDYRSIGGEIGGFQTRHKVYGREGKKCLRCGGRIRRIVVGGRATFYCPSCQREKR"
     gene            9228..9680
                     /locus_tag="PKV64_06095"
     CDS             9228..9680
                     /locus_tag="PKV64_06095"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPN16686.1"
                     /translation="MAFSSLDEILAFAVLREQEAADLYAGLAAAARTEPLRALLRELE
                     AEERRHKILLEKYRAGMPVFSGGPSVPDLGLTDAAVEEPPGPEDSVQDALIRAARKEA
                     QAADLYGRLAAAVSDAAARGLFELLLREELRHKFRLESEYEARILKEG"
     gene            9720..9884
                     /locus_tag="PKV64_06100"
     CDS             9720..9884
                     /locus_tag="PKV64_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019190437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPN16687.1"
                     /translation="MTKWECTACGYIYDPAAGDPDNGIRPGTPFESLPDDWTCPQCGV
                     GKDYFRKVED"
     gene            9906..11108
                     /locus_tag="PKV64_06105"
     CDS             9906..11108
                     /locus_tag="PKV64_06105"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPN16688.1"
                     /translation="MNVVIVGNGLAGTMAAKTIRDLDDRASVVVFAAENRLYYPRPNL
                     IEFIAGRLPEEKLYAFPADWAAKNRIDLRLGTAVRRLHPSEKSVEAADGSIVRYDRLL
                     LADGASSAVPPISGADKPGVFTIRTLDDARALLEYFRTHPRVAVLGGGLLGLEIARAL
                     RLRGAEVTVAEIFPSLLPRQLDPPGGEILKARLEETGLRVLLGVAAAEITGESEARGI
                     VFKDGTRLDADVVVLAAGVRPNTALAREAGLAVDRGVTVDDRMATSAPDIFAAGDGTE
                     HRGRLYGIIPASFAQSRTAAENICGRTKIYEGTVPSNQLKVSDLPLLSAGIIRPEGPG
                     FEEFRLSRPASGVYKKIVLKDGVLAGAVWMGTKTGAAEIAAAVAAGKNAAAWKADILE
                     DGFDFKRL"
     gene            11105..12142
                     /locus_tag="PKV64_06110"
     CDS             11105..12142
                     /locus_tag="PKV64_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16689.1"
                     /translation="MKHIRLSAALPALALALALAGPAAAQDSTGLKVNGYFSFDILRG
                     PSGLPASAWSVANLRGGLIFSGTLTPGLVFILEPTFAQGEAVGLTEARAGLAVAKGVS
                     VTAGLFLVPFGKYNRSRRPHETALISDPPAVGTVYPVNWREMGIEAEASFGNFNAAVF
                     AGNGLAEGADFGAGQQYSDNNKNKGWGGRLGAALSASLEVGGSYYRGKADAANERAVT
                     MVGADASWRTENIRASGEYARATIENPAPFSKGTAEGWFGAVELHWGSWIPVVSYRRF
                     RAEDPFHGPGFAGPDTPGAGLSRNGTQWALGLVYSPAPSIFIKVEVDRGREQGGDAWQ
                     STFRAQAAFYF"
     gene            12160..13491
                     /locus_tag="PKV64_06115"
     CDS             12160..13491
                     /locus_tag="PKV64_06115"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPN16690.1"
                     /translation="MANIVILGTQWGDEGKGKIVDLLTPAFGAVARYQGGHNAGHTVY
                     LNGRKVVLHVIPSGILHPGTLCLIGNGVVIDPRAFLDELEALKSVVPVGEDSIAVSRT
                     AHLILPYHGLLERAAEAALGEKKIGTTCRGIGPAYEDKIGRRGIRAGDLCDPALLREK
                     IFRNTAAKNEIAALWGLEPLDPEKLFAQYADYGARMAPYIKDVSRLLHETLRAGTPVL
                     FEGAQGVLLDIDHGTYPYVTSSNPTAGGVCTGLGVGPTVIDAVLGVIKAYTTRVGSGP
                     FPTELFDETGKLLARRGDEFGATTGRPRRCGWFDAVAAAYACRINGISRVIMTKPDVL
                     DEFPEIKICTAYRYKGSSLRDYPVETEVLEKVVPVYETVPGWKTPVRGVREFRDLPAG
                     FKDFLKLVEDRIGAEAAMISTGVERRETLFRDDLLAGWIDLAAVRKGIAGA"
     gene            13525..14682
                     /gene="alr"
                     /locus_tag="PKV64_06120"
     CDS             13525..14682
                     /gene="alr"
                     /locus_tag="PKV64_06120"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPN16691.1"
                     /translation="MSGPCDGRFWQWIEIERKALASNVRRFRALAGPRRLLLAVVKAN
                     AYGHGLIETSGPALAAGVDWLGVHSAEEGLALREAGLGCPILMLGPAAPGRIEEAVRQ
                     DLRFTVYDRAAAEGISAAAVRLGKTARIHVKVETGTNRQGLPVAEVVSFFARLARLPG
                     LEIEGLSSHFANIEDTTSLAYPRRQMALFNEAAAAVREAGYPIPIKHIAATAAAIVFP
                     ESLCGLIRMGIGLYGLWPSKETYLSCRLRKRRPFVLKPVLSWRARIAQIRRLSKGAFI
                     GYGCSYRTTRPTRLAVIPVGYADGYDRGLSNAAYVLLRGKRAVVRGRIAMNFFTVDVT
                     DIPGAAAGDVVTLIGRDGRETVSAEALAALAGTIGYEILARLNPAIPRRVI"
     gene            14783..15742
                     /locus_tag="PKV64_06125"
     CDS             14783..15742
                     /locus_tag="PKV64_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPN16692.1"
                     /translation="MKRIAVALALVLAIGGLALSAETTVLKVKVQTANIRTQPDLNAA
                     VIKQVKMGTLFESSQTAGDWYEISVTNDLGVTLTAYVHANVVDVISGPGLAVQPEAQP
                     VVRPAPAPARTPSYERRPAPETTYPGGFKIMAAYGMASLTYSEDPAEEYNEYKKSLMG
                     LAGGVGFYSGGLLGVDVGLMYLPKGIRYEGSLSGSDFDVKMRISEISVPVLLRFNLPV
                     QGINPYLLGGGEIAYVAQAKYDYTISGGGDSDSGSKDIKDDVASIDYGLVFGGGLGIS
                     LSGINLVAELRYHLGLANLVKDPEEGDPTVKSNMLVFLAGFRF"
     gene            15876..17969
                     /locus_tag="PKV64_06130"
     CDS             15876..17969
                     /locus_tag="PKV64_06130"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HPN16693.1"
                     /translation="MTTAAAAGFSANALRILKARYFLKNEKGELADASPADLFLRVAR
                     AVAAAERSARDREAWAGKFFDLMTARDFLPNSPTLTGAGRDMCLSACFVLPIEDSMES
                     IFDAVKNAALVHKEGGGTGFDFSRLRPRGSFVRRTQGVASGPVSFLKVIDAATEAVKQ
                     GGTRRGANMGILRVDHPDIENFILMKKDGRSVGNFNISVAATDAFMEAVKKGTSYEIF
                     DPRSKKAVDRKDARKIFRMIVESAWAIGDPGLIFIDRINKANPTRSQGPIAATNPCGE
                     QPLHPYESCNLGSINLANFFAPAGKDGFDWDRFAGVIAAAVRFLDDVIDVNKYPLPEI
                     EAMSKGNRRIGLGVMGWADLLIKRKIRYDSPEALALADKIAAFLRARAEEESSRLAKI
                     RGDFPNIAKSIYKGRRMRNATVLTIAPTGTISRIAGCSSSIEPVFAFTFTSRILDAEL
                     KDVHPLYAEWAEKNPGKEPPAYFVTAHDISPEAHLRMQAAFQKHVDNSVSKTINLPHA
                     ASLKDVETAYLRAFDLKTKGITIYRDGCREEQVLVKASGPAPRIPEERPDTLTSVTDK
                     IKTGFGNLYITITFFNQKPFEVFASIGKSGYSTMADAEAIGRLLSLALRSGVDPKELV
                     SQLKGIGGSEPIFTEGGLVTSIPDAVARVLERHFGAAKNGARDMYQSQCKQCGATLPD
                     EKCPVCPNCGWNKCS"
     gene            18258..18479
                     /locus_tag="PKV64_06135"
     CDS             18258..18479
                     /locus_tag="PKV64_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPN16694.1"
                     /translation="MKDKVEAALNKIRPQLQMDGGDVELVGIDPDGVVKVRLKGACGG
                     CPMSQMTLKMGIERILKKEVPEVKEVVSL"
     gene            18503..19690
                     /locus_tag="PKV64_06140"
     CDS             18503..19690
                     /locus_tag="PKV64_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPN16695.1"
                     /translation="MKRVYLDHIAAAPLAPGVLEKMRPFLEAGFGNPQSLHSDGQAAA
                     EAVEESRERVAALIGAAPPEIIFTASGTEANNLAVKGLAQAQRAKGNHLVVSAVEHAS
                     VLNPVKTLEKSGFQATIVPVDRQGRVDPAAVAGALREDTILVSVMTANSEVGTIEPIA
                     EIVRAVKAKSPGVLVHTDAVAAAGNIPLDVEALGVDALSLSGNAFYGPRGSAALFLRK
                     GTKLVPQIEGGVQEGGRRGGTEDVPAIAGMGEAARLVAAAMPERTACMTALRDRLLDG
                     LPRRVERVHPTGSREHRLPYHASFCVEFVEGESLLLSLDFKGIAASSFSACTSKSLKV
                     SHVLLAMGLDHALAQGSLIFSLVETTSETDIDAVMDAFPPVVQRLRDMSPLYTEFLKE
                     TRR"
     gene            19687..20277
                     /gene="nifU"
                     /locus_tag="PKV64_06145"
     CDS             19687..20277
                     /gene="nifU"
                     /locus_tag="PKV64_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HPN16696.1"
                     /translation="MIYSDKVMDHFQNPRNVGTLPDADGVGQVGNPVCGDMMTFYVKV
                     KDDRLTDIKFQTFGCGAAIAVSSMVSEMALGKTVQEAMDITNEKVAEELGGLPKNKMH
                     CSNLGADALHAALENYQARKAGTASGPAAEAAAGRAVEGAAPDHCPYCDGPVQGPPNM
                     CSCKTCDLDLVECGHCHLYTNGAETRCTHCGAQLER"
     gene            20282..>20511
                     /gene="nadA"
                     /locus_tag="PKV64_06150"
     CDS             20282..>20511
                     /gene="nadA"
                     /locus_tag="PKV64_06150"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014849142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPN16697.1"
                     /translation="MTASPAPPGPSSWIRRVRDLAREREAVLLAHNYQRPEVQEAADF
                     VGDSLELSRKAAGVEGRVIVFCGVRFMAETAAL"
BASE COUNT         3592 a         6502 c         6747 g         3570 t
ORIGIN      
        1 catcctggac gtttttctca tcgcctccgg attcattctc cgggtcgtcg ccggcggggt
       61 ggtcctccgc gtctccctgt cctcctggct gatcatctgc acctcgcttc tggccctctt
      121 catcggtttc ggcaagcgcc gtcatgagat cgtcttcctg gaggaggacg cgtctctcca
      181 ccggtccatt ctccgggaat acagccctct tcttctcgac caaatgattt cggtcgtcac
      241 tgcctcgacg gtgatcgcct acagcctcta caccgtttcc gaggagaccg tccgcaagtt
      301 cgggacgtcg aacctgatct tcaccatgcc gttcgtcctc tacggcttgt ttcggtatct
      361 ttacctcgtc catcagcggg ggcagggggg aaatcccgag gagatgattt tccgggacaa
      421 gccgctggcc gcgaccatcg tcctctggat cgtctccgtc gttatgattc tttacgtccg
      481 ctgagcccgt tttcgtcctg aagccagcgg cggctcttcg gttcccgctc caggtggaag
      541 acgatcatcg cttgcaaggc gccggcgaag gagcggatct ctccggccga aaaagggagg
      601 gaggcgcagg cggacggcgg attgtcccgg acccaggaga ggaaccggcc caggctttcc
      661 cggatctctt ccttccgcga gggcgcgcag gcgtcgcaga aaacgccgtc ccggcggggg
      721 gacagccagg ccggagccgg atcctcgatc gcccgccggc agaggcggca gcgccggatg
      781 tccggcagga ttccgttgag acggaggagc caggcttcga aatatcgggt caggatctgg
      841 acgtcgacct tgtcccggac ggcccggagg atcgagagaa agaggcggaa ggccaggttc
      901 tcccgggctc ggaccggaac gaattcctcg gataattcag ccatgtagga cagggcgaag
      961 ccggccgccg gatcggagaa gacgtcgaag aacgattcgc ggaggtcgca ggagttgatc
     1021 gtgaccaggt cgctccgttc cttctcgtaa aagaacacgg tgacgtggga caacggctcg
     1081 agggagcttc cgaaccggtt cgcgaatttc cgggccccct tggccacgcc gcgaaggacg
     1141 ccccgctcgg ccgtgagaaa aacgacgatc ttgtcctgat cggccaaggg aaacgtccga
     1201 aggacgatgg cttcagcctg atcgcggggc atgcggcctc agggaagcag ggtgatgcgc
     1261 aggtcccgga tcttggcccg aagattctcc ggcgtgattt gaagctcggc cgaggcccgc
     1321 gcttcatccc attggtgccg gcggagcgcc tggtgcacgg cctcgcgctc ggccctccgg
     1381 acggcctcgg ccagggaggg ccgggaggcc ggcatcggtc cttgctcgcg ggtttcgacc
     1441 agaaggttca aatggccggg gccgatgact tgatcctcga cgaggatgac gaaacgttcc
     1501 aggacgttca tcaactcggc gacgttgccc ggccaagagt agttgacgaa cgcctggagc
     1561 gcctccggat ggatggattt gggcatccgg ccgtattcgc ggcaatagat cctcaggaaa
     1621 taggcgatga ggaccggaat gtcctcaagc cgctcccgca gcggcggcag cgtcagggga
     1681 atgacgttga tcttgaaata cagatcctcg ttgaagcgcc cctgacggac gaggtcgccc
     1741 aagctggcct tagtcgaggc gatgacccgg gcgtcgaacg gaaccggctc cgagccgccg
     1801 agcggctcga aagatccctt tgccagggcc aggttgagag ccgcctgggt ccgaagcgtc
     1861 agggtctcga tgccttccag gaacagcgtc cctccgccgg cttggacgat ctttcccttt
     1921 ttttccttgg ccgggtccgg tccgtggccc ttgaggcacc cgaagagctc ggcgtcgatc
     1981 agggtttcgg ggagggcggc gcagttgact tccacgaacc gccgatcccg ccgcgggctg
     2041 cgctgatgaa tgaggcgggc ggccagctcc ttgcccgttc cgttttcgcc ggagatgagg
     2101 atccggccgt ccgtcggggc cgccgcccgg attttggccc ggaggttctg aagggcgggg
     2161 cttttgccga ccaggcaatt gcgtccggcc agacgctcgc gcaggcggac gttctcctcc
     2221 tccagccggg cccggaggag ggagttgcgg acggtcagca cgactttttc cagggacaga
     2281 ggcttttcga ggaaatcgaa ggcgcccatc ttgacggcct tgaccgccgt ttccacggtc
     2341 ccgtggccgg agatgaccac caccgccgga cggtcctcgt ggcgctggat ctcggccaac
     2401 acatcgaggc cgttcatctc gggcagccag atatcgagaa tgaccaggcc gaagctttcc
     2461 ttggacaggg ccgccaatcc ctcctcgccg gaggcggcgg tccggaccac atacccttca
     2521 tcctccagga ttccccggag agaggaccgg atggccgact cgtcgtcgat gaccagaacg
     2581 cgttcttgac tcatgcaggc agctcgatcg tgaatttcgc tcccatcggc cggttgtttt
     2641 cggcgcggat gaggccgtca tgctcgcgga tgatttggct gacgatggcc aggccgaggc
     2701 cccggccttt tttctttgtc gaaaaagtcg gcaggaagat cttttccggg tcctcgacgg
     2761 agattcccgg ccccgtgtcc gtgaccgtcg cccggacgcg gcgggaatcg cggtcgtaat
     2821 ccgcttgaag agtgattgta ccttttttat tcatcgcgtc aatggcgttg tcgatgaggt
     2881 tgatgaacac ccgcttgatc tgctcggcgt cgagctggat ttcggccggg acgtccgggg
     2941 cgatccggat ctcgaaaacg atgtcggcgt aaatccctcg gaagagattg gcgacctgcc
     3001 ggaggatgtc gccgagggag gccggctgga gatggatctt gggcagccgg gcgaaatccg
     3061 agaactcgtc caccagggcc ttgatcgtct gggcttcctg aatgatcacc cgggcgcctt
     3121 cggcgacgac ctctccgccc gacggatcgc ccttgtggat gttgcggatg atgcgctcgg
     3181 cgttgagctg gatcggcgtc aagggattct tgatttcatg ggcgacccgc tgggccactt
     3241 ccttccaggc ggcgatcttc tgggccgtga tgagctgggt caggtcatcc acggccacga
     3301 tcaagccgga aaagtcctcc tccggcttgc gaaggggcgt caggttcagg gacagggtct
     3361 tcgtctgcac gcccatggtc atcgggatct cgcggtcgct gatcctggcc cgattgacga
     3421 aggccgtctc gatggccgcc cgcagcgccc ggtagcgctc ggaggcgagg agatcccggt
     3481 acgggcgccc ggtcgcgttt ttttccggca ggtccagcat ctcccgggcg gccgggttga
     3541 tggtcgccac gatgcctcgg gcgtccaggg cgatgacccc ggtcgaaatc gcattcagga
     3601 cgatttcgat cgtccgctgg cgggcggcca cttcggccgt tttggccgcg acctccgcct
     3661 cgcccttccg caggtcggcg gtcatctggt tgaaggagtc gatgagggtc ccgatttcgt
     3721 cggaggcggg atcctcgatc ttgatcgaca agtttccgcg cgagacttcc ttggtcgcgt
     3781 aggccagttt ttcgatggga acggtgatgc tcttggcgat atgaaagccg atccaaatgg
     3841 ccgtgaagac gatgatcagg gtgacgaaga tcaaggtcag ggtgtagagc gtcttcgtca
     3901 gatcccgctg ggtcccccgc atgcggtagc gttcgtcgag ggccatgatc gagttcattt
     3961 tctgcgttag gttctgggga aggaatttgc cggccgtcac caggaccgct cccatctttg
     4021 ggtcgatgaa cgacacccct cgccggacga actcgcccgc ccccatcggg gtgatgtcgt
     4081 tgaggtccaa gccgagatgg gccttgcgga cctcgtcggc gttgaggtcc cgatagtcct
     4141 ggaggggaag attcggattg agaagggaaa acagctcctc ccccccgaga aagaggctga
     4201 tttcgtccag ccggtattcg ttcagcttcc ccttgacgaa atcctccaag cgcggccgcc
     4261 gggcgggatc ggccagctcg gaccgctgga tttcccgggc gagctgctgg gcgaaatgaa
     4321 aggtctcgtc cgacgtcgtc gcgtagaatc catcggccac ggcccgggtg tccttgacca
     4381 ggcgggtgat atcgaggcgg aaccactgct cgatgctgtt gttgatgatc cggctggtga
     4441 agatgaaaag gagaagcgtg gggatgaagg aaagggcggt gaagaagatg acgagcttgg
     4501 tcttgaaatg ggagccgacg accttttgct tgcgctcgag gtaaagcttg accaaatacc
     4561 ggccgagaac gaagaacagg atcaaggcca gcagaaggac gatgaactgg agggcggcca
     4621 ggagaatgga ggtgaccttg cccggggaga attcggccga accgcggagg aagacgtcga
     4681 tgacgaagaa gaggatgacg agaaggagga tgaccgagac gacgacccgg gtgcccttct
     4741 tctgccgccg gccctggttt tcgtccatgg gcgtcagcgg acggcgccgc cggccggcgg
     4801 attctcctcc cggaggaatg tggtcgaatt ctcgcgggtg atgcggatat ccgtcaggac
     4861 gacgccgctt ccgcccaggg gccgcccggg gcgtccgttc aaggtgaagt cgaagccgcc
     4921 ggcattgccc gtctggagga tgaattccgt ttccgcccgg agcgaggccg tcgatccggc
     4981 cggcttgatt ccgtccagga cgacgacgcc gtcggccgag atgtgcatcc aggaatcggc
     5041 gttgaaacgg agctcgagac ggaggcccgt ttcggccggg acgggctcgg gttccttggc
     5101 cgcggccggc tcgggcaggg cggcctcggt ctgcggggcc ggaatctccg tcgaagaaac
     5161 cggcggagcg gatttttccc cgggcttcag gaaaagcaga tatccggccg cggccgcgcc
     5221 gaggacgagc aggaccgcga gggcggggac gagaattcgg cgcgttcccc cggcctccgc
     5281 gccgcgcttc tttcgttttg gagattcgcc ctcgttcccg ggggattcca agcgggcctt
     5341 ctcctgttcc tcatagcggg ccaggaaggg ctcgggatcg acgcccatgg cttgggcgtg
     5401 ggccttgatg atgctccgga tgaagaactt ctggggcatt tcctcccagc ggtcctcctc
     5461 gagggcgacg aggtagcgga gcccgacttt cgtccggtcg gagatgtcct tgagggagag
     5521 cgaatgcttc tcccgctctg tccgcaattc ttgtcccagg gtggccatgt gttgagattt
     5581 taggtcaccg gcggggaaga tgtcaatgaa ccggcggctc tatgacgcat cgcgtggaga
     5641 taaaagggcg gagttcggcc ggcggactcg gctgcccaac agtcgttgtt tctgtctgcc
     5701 ccgcggcaag ccgttcacgg aagttaattc gttgatcggg ccggggcgag gatcaggagc
     5761 ggaaaacgcg gcccagggcg gccaggctgc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn ncgggccggg gcgaggatca ggagcggaaa acgcggccca gggcggccag
     5941 gctgccgaga agaccggcca agcccccgcc ggcgacgagg ccggcggctt ggaggaggga
     6001 gagcggctcg aagccgatga gatcctggag agcgcccagg gaatggccga gatagagcgg
     6061 aaagagacga gccgcgagca ggaccagtcc ggccgcgacc gtccccccgg ccactcccag
     6121 gatcaggccc tcgaggagaa aggggccccg gatgtagccg ttcgtcgccc cgacgaggtg
     6181 aaggatggag atttcctcgt gccggctcag gatgttgagg cggaccgccc cggagacgac
     6241 ggcgatggac atcaggatga gaaggccgcc gacgacgacc ccgacggcgt cgctcaaccg
     6301 cccgagggcc cggacgcggt ccgaccagcg gcggttgaac tggacgtcct ccacccccgg
     6361 aagccgccgg aggccgtcga tcatccggtc gatgggcgcg tccgggccgg ccggatcgcg
     6421 caggatcgcc tcgatcgagg ccgggagcgg attgcggccg aggttttcca ggacgtcccg
     6481 aagatcgggg aattccttga gaaaccggtc gcccgcttcc tccggggtga ccgtccggac
     6541 cgaggcgacg aggggcgaca ggcggacccg gtcctcgatc gtccgccgct cggcggccgt
     6601 ggccgacgga ctgaggtaga cgacgacggc gacatcccgg gagaggatcc gggcccggct
     6661 ccggaggttg ttcgaaagag agagaaagag gccggccatc agaaaagaga ggacgaggac
     6721 ggcgaaagag acggcgtggc gggtcttgcc ccggcgcaga tgccccccgg cgtcccggaa
     6781 cgcccgcccg atcttccgcc cgttcacggc gccgtccccc gctcccgcat ccgtccttcc
     6841 cggagggcca gaacgcggcc ggcgaacttg cggacgattt cccggtcatg cgtgcagatg
     6901 acgacggtcg tgccctggcg gttgatttcc cggaacaagg cgatgatttc cgaggcgagg
     6961 tcgggatcca ggtttcccgt gggctcgtcg gccaaaagaa ggcggggatt gttgacgacc
     7021 gcccgggcga tggccacccg ctgttgctcc ccgtaggaca ggccggccgg cgtattccag
     7081 atccgatggt ggagcccgac catgcggaga gcgaggaaaa ccttgcgctt gacgtccttg
     7141 gacggcaggc cgaggacgcg gagggggacg gcgacgttgt cgaagacccg gcgctcggcg
     7201 agcagacgga aatcttggaa gaccacgccc atcgcccggc ggaggatgta gatcttggcg
     7261 gaggggatgc ggcggatatc catcccttcg atcaggatcc gcccgtcggt gggcgggcat
     7321 tcccgggtca tcagccggag gatcgtcgat ttccccgatc cgctcggccc ggtgatgaag
     7381 accatctccc cggccgcgac ggcgaaggaa acgtcggaga gagcccagtc cggtttatga
     7441 tactgcttcc aaacgtgact gaactcgatc atcgaggccc cggccggacc gattatacca
     7501 tcctcacagg gtgaaaaggt acatcagcca gccgaacaaa acggcccctc cggccatgcc
     7561 tccgaacatg atcaggccgt atttcaggcg gctttttgcc tcgtcccggc ggaggaaggc
     7621 caggacgacc gcgacgaggg ccgcatatag gatcatcgag agaaggtggc tgcggaaaat
     7681 catgactttg tccccgcggc gatgtcgtaa gcatcgaggg cgacgaggaa attcagaagc
     7741 cccgccccgg ccaagtacgc ggttccgaat tcgaacgtcg ccagcttcag gtcgccgatc
     7801 ccgacgggca ggacgcggga gagaagatag agaagcccgt tgccgatgtc ggcgaagaag
     7861 gccaggatcg tcaggggatt ttccgtttgg aaagaataaa tccgtccgcc cagggccagg
     7921 ccgagggccg tcatggccgc caggcagccg aaaaagaccg ccccgcggag gtatttcctc
     7981 tggagaaaat ggccgaggcc gggcacggcc cagccgagaa ccatcaggac gacggctttg
     8041 attttcatga cggaggacat cttagcagtc tttttttaga gacacaagag ccgtgtcccg
     8101 gatctccggc cggccgggcg ggggatctcg ccgggagcga aaagggaatg acggccttgt
     8161 gtctccggcg ggatcggtta caataccgga atgcctgagc tgcccgaggt cgagaccatc
     8221 gccgccgccc tgaggtcggc gatcgtcggc cgggaagtcg cggccgcccg ctgtttttta
     8281 cccaagctcc tgcgggcgac ggagccccgg gatctcgatg ccctggccgg ggcgcggatc
     8341 ctggccgtga ggcggcgggg caagatcctg atcctcgagg cgcctccccg ggcccttctt
     8401 ttccatctca agatgaccgg ccggttcgac tgggagaacg ccggccgtcc cctctcgaaa
     8461 cacacgcatt tcgttctctc tttcgccggc tcggacggag agcttcgctt tcgggacgtc
     8521 cggaaattcg gcttcctccg ctgcctgccg cgcggagagg tcgaggcctg cgacgagatc
     8581 cggcggctcg gccccgaacc tctggacatc ggcccggaag aattcgccgc ccgcctggcc
     8641 gcccgcagag gccggctcaa aagcgtcctt ctcgaccagc gcgtcctggc cggggtgggg
     8701 aatatctacg ccgatgaagc cctccacgcg gcccgccttc atcccctgac cgcggccggc
     8761 cgcctgcgtc cggacgagtt gcgccggctg cgggcgtccc tccgccgcgt tctggcggcg
     8821 gcgatcgccg ccggcggctc gagcatccgc gattaccgga gcatcggagg ggagatcggc
     8881 ggctttcaga ctcgtcataa agtctatggc cgggagggga aaaaatgcct tcggtgcggg
     8941 gggcggatcc ggcggatcgt cgtcggcggc cgggcgacgt tttattgccc gtcctgccag
     9001 cgcgaaaaac gctgaaggcg ggaagccgcc gctcttcccc cgcgatggtt ccggggggtt
     9061 ctcgttcaga atgatcgtcg acgctcccgg cccgggattg ggggacgtcc ccggaatgag
     9121 gggaaagagg atgcgaatcg ctcgggccgg cttggggttg agcgaggcgt ccctgaattg
     9181 ttataatgcc tgggccggtt ccaacttcat tcgcgggaga gtgcgacgtg gccttttcat
     9241 ccctggatga aatcctcgcc ttcgccgttc tccgcgagca ggaagcggcc gacctctacg
     9301 ccggtctggc tgcggccgcc cggaccgagc ctctccgcgc ccttttacgg gagctggaag
     9361 ccgaggagcg ccggcacaaa atcctcctgg aaaaataccg ggcgggaatg ccggtctttt
     9421 cgggcggacc ctcggttccg gacttgggac tgaccgacgc ggccgtggag gaaccccccg
     9481 ggccggagga ttcggtccag gacgcgctga tccgcgccgc ccggaaggaa gcgcaggcgg
     9541 ccgacctcta cggtcggctg gccgccgccg tttcggacgc cgcggcccgg ggcctgttcg
     9601 aattgctcct ccgcgaggag ctgaggcaca agttccgcct ggaaagcgaa tatgaagcgc
     9661 gcatcctcaa ggaaggatga gcgcccggcc tctgtcgatt catttgaaag gagccaccga
     9721 tgacgaaatg ggaatgcacg gcgtgcggct atatttatga tccggccgcc ggcgacccgg
     9781 acaacggaat ccgtccgggc acgcccttcg aatcccttcc cgacgactgg acctgtccgc
     9841 aatgcggggt gggcaaggac tatttccgga aagtcgagga ctgagcggcg gccgccttct
     9901 tctccatgaa cgtcgtcatt gtcggaaatg gtctggccgg aacgatggcc gccaagacca
     9961 tccgcgatct ggatgaccgc gcttccgtcg tcgttttcgc cgccgagaac cggttgtatt
    10021 atccccggcc gaacctgatc gaattcatcg ccggccgcct gcccgaggaa aagctctatg
    10081 cctttccggc cgactgggcg gcgaagaacc ggattgatct ccggctcggc accgccgtcc
    10141 gccgtcttca cccctcggaa aaaagcgtcg aggcggccga cggatcgatt gttaggtacg
    10201 accgcctcct cctggccgac ggagcctctt cggccgtccc cccgatctcc ggggcggaca
    10261 agcccggcgt cttcaccatc cggacgctcg acgacgcccg ggctctcctg gaatatttcc
    10321 ggacccatcc gcgcgtcgcc gtcctgggcg gaggcttgct cggcttggaa atcgcccggg
    10381 ccctccgcct ccggggggcc gaggtcacgg tggccgagat cttcccctcc cttctgccgc
    10441 ggcagctcga tccgccgggc ggagaaatcc tgaaggcccg tctcgaggag accggcctcc
    10501 gcgtgctcct gggcgtcgcg gccgcggaaa tcaccggaga gtccgaagcc cgcgggatcg
    10561 tcttcaagga cgggacgcgc ctggacgccg acgtggtcgt cctcgccgcc ggagtccgcc
    10621 cgaatacggc cctggcccgc gaagccgggc tggccgtgga ccggggcgtc accgtcgatg
    10681 accggatggc gacgtcggcg cccgatatct tcgcggccgg agacggaacc gagcaccgcg
    10741 gccgcctcta cggcatcatt ccggcctcgt tcgcccagtc gcggacggcc gcggagaaca
    10801 tctgcggccg gaccaaaatt tatgagggga cggtcccctc caaccagctg aaagtatccg
    10861 acctgcccct cctgtcggcg ggaatcatcc ggccggaagg gccgggcttt gaggaattcc
    10921 ggctgtcccg gcccgcttcc ggcgtttaca agaagatcgt cctcaaggac ggcgttctgg
    10981 ccggggcggt ctggatgggg acaaaaaccg gcgcggccga gatcgccgcg gccgtcgccg
    11041 ccggaaagaa cgcggccgcc tggaaagcgg acatcctcga agacggattt gatttcaaac
    11101 gcttatgaaa cacattcgct tgtccgccgc tctcccggcc ctcgccctgg ccttggccct
    11161 ggccggaccg gccgccgccc aggactcgac cggcctcaag gtcaacggct atttttcctt
    11221 cgacatcctc cggggaccct cgggccttcc ggcttccgcg tggagcgtgg ccaatctccg
    11281 gggggggctg atcttttccg ggactcttac gccgggcctc gtcttcatcc tggagccgac
    11341 gttcgcccaa ggcgaggcgg tcggcttgac cgaagcccgg gcagggctcg ccgtcgccaa
    11401 gggcgtcagc gtcacggccg gtctctttct cgttcccttc ggaaaataca accggtcccg
    11461 ccggccccat gaaaccgccc tgatctccga tcccccggcg gtcggaaccg tttaccccgt
    11521 gaattggcgg gaaatgggaa tcgaggcgga agcctcgttc ggcaatttca acgcggccgt
    11581 tttcgccgga aacggcctgg ccgagggagc cgatttcggc gccggccagc agtactccga
    11641 caacaataaa aacaagggct ggggggggcg cctgggggcc gccttaagcg cttccctgga
    11701 agtcgggggg tcttattacc ggggcaaggc cgacgcggcc aacgagcggg cggtcaccat
    11761 ggtcggggcc gacgcctcct ggcggacgga gaacatccgg gcttcgggcg aatacgcccg
    11821 ggcgacgatc gaaaatccgg cccccttctc caagggaacg gccgaaggat ggttcggcgc
    11881 cgtcgagctt cactggggct cctggatccc ggttgtttcc taccggcgtt tccgggccga
    11941 agatcccttc cacgggccgg gattcgccgg tccggacaca cccggcgcgg ggctctcccg
    12001 gaacggaacg caatgggccc tgggtctcgt ttattctccg gcgcccagca tcttcatcaa
    12061 ggtcgaggtc gaccgggggc gggagcaggg cggagacgct tggcaatcga cgttccgggc
    12121 ccaagcggcg ttttattttt aaggatcaag aggcgcgtca tggcgaacat tgtcattttg
    12181 ggcacccaat ggggcgacga ggggaagggg aagatcgtcg atctcctgac tccggccttc
    12241 ggcgcggtcg cccgctacca gggaggccac aacgccggcc acacggtcta cctcaacggc
    12301 cggaaagtcg tcctccacgt gattccctcg ggcatcctcc atccggggac gctctgcctc
    12361 atcggcaacg gcgtcgtgat cgatccccgg gcgttcttgg acgagctgga agctctgaag
    12421 tccgtcgttc cggtggggga ggactcgatc gccgtcagcc ggacggccca tctcatcttg
    12481 ccgtatcacg gcctgctcga gcgggccgcc gaggcggctt tgggcgaaaa gaagatcggc
    12541 acgacttgcc ggggcatcgg cccggcgtac gaggacaaaa tcggccggcg gggcatccgg
    12601 gcgggggacc tttgcgaccc ggccctcctc cgggaaaaaa tcttccgcaa caccgcggcc
    12661 aagaacgaaa tcgccgccct gtggggcttg gagccgcttg atccggaaaa gctcttcgcc
    12721 caatacgcgg attacggggc ccggatggcg ccctatatca aggatgtgtc ccgccttctc
    12781 cacgagacgc ttcgggcggg aacgccggtc ttgttcgagg gagcccaggg cgtgctgctg
    12841 gacatcgatc atggaacgta tccgtatgtg acgtcctcca atccgaccgc gggcggcgtc
    12901 tgcaccggcc tgggcgtcgg gcccacggtc atcgacgcgg tcctgggagt gatcaaagct
    12961 tacacgaccc gggtcggatc cgggccgttt ccgaccgagc tcttcgacga aaccggcaag
    13021 ctcctggccc ggcggggcga cgaattcggg gcgacgaccg gccggccgcg gcggtgcggc
    13081 tggttcgacg ccgtggccgc cgcctatgcc tgccggatca acgggatcag ccgggtgatc
    13141 atgaccaagc ccgatgttct tgacgagttc cccgagatca aaatctgcac cgcctatcgc
    13201 tacaagggct cgtcgctccg cgattatccg gtggaaacgg aagtcctgga aaaggtcgtc
    13261 cccgtctacg aaacggtccc tggatggaaa acgccggtcc gcggcgtccg ggaattcagg
    13321 gatctgccgg ccggtttcaa ggatttcctg aagcttgtcg aggaccggat cggggcggaa
    13381 gcggccatga tatcgacggg agtcgagcgc agagagacgc tgttccggga cgatctcctg
    13441 gcgggatgga tcgacctggc cgccgtccga aagggaatcg ccggcgcctg agcgccggga
    13501 ccgacgccgg gcaggaggca tccgatgagc gggccatgcg acggccgctt ctggcaatgg
    13561 attgagatcg agcgcaaagc cctggcctcc aacgtccggc gtttccgggc cctggccggg
    13621 ccgaggcggc tgctcctggc cgtggtcaag gcgaacgcct acggccacgg cttgatcgaa
    13681 acctccggcc ccgccctggc ggccggcgtc gactggctcg gcgttcattc cgcggaggaa
    13741 ggcctggccc tgcgcgaagc cgggttaggc tgccccatcc tgatgctcgg gcccgccgct
    13801 cccggccgga tcgaggaggc cgtccggcag gatctccggt tcaccgtcta cgaccgggcc
    13861 gcggccgagg ggatatcggc cgccgccgtc cgcctgggaa aaacggcccg gatccacgtc
    13921 aaggttgaga cggggacgaa ccgccagggc ctcccggtcg ccgaggtggt ctccttcttc
    13981 gcccggctgg cccgtcttcc cggtctcgaa atcgagggct tgtcctctca tttcgccaac
    14041 attgaggata cgacctcgct tgcctatccc cggcgccaga tggccctctt caacgaagcc
    14101 gcggccgccg tccgggaggc cggttatccg atccccatca agcatatcgc ggccacggcc
    14161 gcggcgatcg tctttcccga gtcgctttgc ggcctgatcc ggatgggaat cgggttgtac
    14221 ggcctctggc cgtcgaagga aacgtacttg tcctgccggc ttcgcaagag gaggccgttc
    14281 gttcttaaac ccgttctttc ctggcgggcc cggatcgccc agatccggcg cctctccaag
    14341 ggggcgttca tcgggtacgg gtgctcgtac cggacgaccc ggccgacgcg gctggccgtc
    14401 attccggtcg gatacgccga cggctacgac cggggcttgt cgaacgccgc ctacgttctt
    14461 ctccgcggaa agcgggccgt cgtccggggc cggatcgcga tgaatttttt cacggtcgac
    14521 gttactgaca ttcccggggc ggcggccggc gacgtcgtca ccctgatcgg ccgtgacggc
    14581 cgggaaacgg tctcggccga ggccctggcc gccctggccg ggaccatcgg gtacgagatc
    14641 ctcgcccggc tgaatccggc catcccccgg cgggtgatct gatcggtttt tcggttttcc
    14701 cgcttttcct cccccttgct atttttttat agtcatggca taaaggaccc gatcctacac
    14761 ggaatctgac tgggaggagt gcatgaaacg catcgccgtc gctctcgccc ttgtgctcgc
    14821 gatcggagga ctggccctgt ccgcggaaac gaccgtcctg aaggtgaagg ttcagacggc
    14881 caacatccgg acccaacccg acctcaatgc cgccgtcatc aagcaagtca aaatgggaac
    14941 cttgttcgag tcctctcaga cggccggcga ctggtacgag atttcagtga cgaacgatct
    15001 cggcgtcacc ttgaccgcct acgtccacgc caacgtggtc gacgtcattt ccggccccgg
    15061 cctcgccgtc cagccggaag cccagcccgt cgttcggccc gccccggccc ccgcccggac
    15121 gccgtcctac gagcggcggc cggcgccgga gacaacctat cccggcggtt tcaagatcat
    15181 ggccgcctac gggatggcca gcctgaccta ttccgaggac cccgccgagg aatacaatga
    15241 gtataaaaaa tccctcatgg gcttggctgg aggggtcggg ttctattccg gaggcctgtt
    15301 gggggtggac gtcggcttga tgtacctgcc caagggaatc cggtacgaag gatcgctgtc
    15361 ggggtctgat ttcgacgtga agatgcggat cagcgagatc agcgtcccgg ttctcctgcg
    15421 gttcaacctc ccggtccagg gaatcaaccc gtatcttctg ggcggcggcg agatcgctta
    15481 tgtcgcccag gccaagtacg actacacgat cagcgggggc ggcgacagcg actccggctc
    15541 caaggacatc aaggacgacg tcgcctcgat cgattacggg ctcgttttcg gcggcggcct
    15601 ggggatctct ctcagcggga tcaatctcgt agccgagctt cgctaccatt tgggattggc
    15661 caatcttgtc aaagacccgg aggaaggcga tcccacggtc aaatcgaata tgctggtctt
    15721 cctggccggc ttccgcttct aaggctccgt tccggcggac cgtcggcggc cgtcttggca
    15781 aatcgcccga agaggcgtat actaatacta tcattttaat atgataatcg tgcgggcccg
    15841 ccggcttccg ccgcccggcc cggggagcga tgcccatgac gaccgccgcc gcggccggat
    15901 tctcggccaa cgcccttcgg atcctcaaag cccgttattt tctcaagaac gagaagggag
    15961 agctcgccga cgcgtcaccg gccgatctct ttctccgcgt cgcccgggcg gtggccgcgg
    16021 ctgagcgctc ggcccgggac cgggaagcgt gggcggggaa atttttcgac ctcatgacgg
    16081 cccgcgactt cctgcccaac agcccgaccc tgaccggggc ggggcgggac atgtgtctca
    16141 gcgcctgctt cgtcctgccg atcgaggatt cgatggaatc cattttcgac gcggtcaaga
    16201 acgccgccct ggtgcacaag gagggcggcg gaacgggctt cgatttcagc cggctccggc
    16261 cgcgcggcag cttcgtccgc cggacccagg gagtcgcctc ggggccggtc tcgttcctga
    16321 aggtcatcga cgcggcgacc gaggcggtca agcagggggg gacgcgccgg ggggccaaca
    16381 tggggatcct tcgggtcgac catcccgaca tcgagaactt catcctgatg aagaaggacg
    16441 gccggagcgt cggcaatttc aacatctcgg tggcggccac ggacgccttc atggaggcgg
    16501 tcaagaaagg aaccagctac gagatcttcg acccccgctc gaaaaaggcc gtcgaccgca
    16561 aggacgcccg caagatcttc cggatgatcg tcgaatcggc ctgggcgatc ggcgatccgg
    16621 gattgatttt catcgaccgg atcaacaagg ccaatccgac gcggagccag gggccgatcg
    16681 cggcgacgaa tccctgcggg gagcagcccc ttcatcccta cgagtcctgc aacctcgggt
    16741 cgatcaacct ggccaacttt ttcgccccgg ccggaaagga cgggttcgac tgggaccgct
    16801 tcgccggggt gatcgccgcg gccgtccgct tcctggacga tgtcatcgac gtcaacaaat
    16861 acccccttcc cgagatcgag gcgatgtcca agggcaaccg acggatcggg ctcggggtga
    16921 tgggctgggc cgacctcctg atcaagagga agatccgcta cgactccccc gaggccttgg
    16981 ccctggccga taagatcgcg gcgtttctcc gggcccgggc cgaggaggaa tcctcccgtc
    17041 tggcgaaaat ccgcggcgac tttccgaaca tcgccaagtc gatctacaag ggccgccgga
    17101 tgcggaacgc gacggtttta accatcgccc cgaccgggac gatttcccgg atcgccggct
    17161 gttcctcgag catcgagccg gtcttcgcct tcaccttcac ctcccgcatc ctggacgccg
    17221 agctcaagga tgtccatccc ttgtacgccg aatgggcgga gaaaaacccc ggaaaggagc
    17281 ccccggcgta tttcgtcacg gcccacgaca tctcccccga ggcccatctc cggatgcagg
    17341 cggccttcca gaagcacgtc gacaactccg tctccaagac catcaacctg ccccacgcgg
    17401 cctcgctgaa ggatgtcgaa acggcctatc tccgggcttt cgacctgaag acgaagggaa
    17461 tcacgatcta cagagacggc tgccgcgagg agcaggtcct ggtcaaggca agcgggccgg
    17521 ccccgaggat tccggaggag cggccggaca ccttgacctc ggtcacggat aagatcaaga
    17581 ccggattcgg caatctctat atcaccatca cgttcttcaa ccagaagccg tttgaggttt
    17641 tcgcctcgat cggcaagagc gggtattcga ccatggccga cgccgaggcc atcggccggt
    17701 tgctttcctt ggccctccga agcggggtcg atcccaagga actggtctcc cagctcaagg
    17761 gaatcggggg atcggagccg attttcaccg agggggggct cgtcacctcg atcccggacg
    17821 ccgtcgcccg ggtcctggaa cggcatttcg gggcggcgaa aaacggcgcg cgggacatgt
    17881 accagtcgca atgcaagcag tgcggagcga ccctgcccga cgagaaatgc cccgtctgtc
    17941 ccaattgcgg ctggaacaag tgctcgtgac gacggcctgt cgccgtcctt gagagccgcc
    18001 ggatatccgg ctccccggaa cggggacgct ccttttgcct tggggggacg cgaagcccgg
    18061 ccgtccctcg cgaagatttc atccccgatg ggaacaatcc cggcggccgg ccgcccgcct
    18121 ggccgccgcg gcgatcgatc ccggacggcg gggcttgaca tcggcgtgga taaagcctat
    18181 aagaatacta ggaaataata actggaaaaa tcgaatcgaa gcgattatat tatggggacc
    18241 ttatcaagga gaattccatg aaagataaag tcgaagcggc tctgaataaa atacggcccc
    18301 agctgcagat ggacggcgga gacgtcgagc tggtcgggat cgatccggac ggcgtggtca
    18361 aggtgcgctt gaaaggcgcc tgcggcggct gcccgatgtc ccagatgacc ttgaagatgg
    18421 gcatcgagcg gatcctcaaa aaggaagttc cggaggtcaa ggaagtcgtc tccctctgac
    18481 cgcccgcttt tcccggccgc cgatgaaacg cgtttatctc gatcacatcg ccgccgcccc
    18541 cctggcgccc ggcgtgctgg agaaaatgcg tccgttcttg gaggccggat tcggcaaccc
    18601 gcaaagcctt cattccgacg gccaggcggc ggcagaggcc gtggaggaaa gccgggagcg
    18661 ggtggccgcc ctgatcgggg ccgccccgcc ggaaatcatc ttcacggcca gcgggaccga
    18721 agccaacaac ctcgcggtca aaggcctggc ccaagcccag cgggccaagg ggaatcatct
    18781 cgtcgtctcg gccgtcgagc acgcgtccgt cctgaatccg gtcaaaaccc tcgagaaatc
    18841 cggcttccag gcgacaatcg tcccggtcga tcgtcaagga cgggtcgatc cggccgccgt
    18901 tgccggcgcc ctccgcgaag atacgatcct cgtttccgtg atgaccgcca attccgaagt
    18961 cggaacgatc gagccgatcg ccgagatcgt ccgggcggtc aaagccaagt ctcccggcgt
    19021 gctcgtccat accgacgccg tcgcggccgc ggggaatatc cccctggacg tcgaagccct
    19081 gggcgtggac gccctgagcc tttccggaaa cgcgttctac gggcctcgag ggagcgccgc
    19141 cttgtttctg agaaagggaa cgaagctcgt tcctcaaatc gaagggggag tccaggaagg
    19201 cggccggcgc ggcgggaccg aggacgtgcc ggcgatcgcc gggatgggtg aggccgcccg
    19261 cttggtggcc gcggcgatgc cggaacggac ggcctgcatg acggcccttc gcgaccggct
    19321 tctggacggc ctgcctcggc gggtcgagcg cgtccacccg accgggtccc gggagcaccg
    19381 gcttccttat catgccagct tctgcgtcga attcgtcgag ggagagtcct tgctgctcag
    19441 cctcgatttc aaggggatcg cggcctcgag cttctcggcc tgcacgtcca agtcgctcaa
    19501 ggtttcccac gtcctgctgg ccatggggct ggaccacgcc ctggcccaag ggtcgttgat
    19561 tttcagtttg gtcgaaacga cctcggaaac cgacatcgac gccgtgatgg acgccttccc
    19621 gcccgtcgtt cagcgtttac gggatatgtc ccctctttac accgagtttc tgaaggagac
    19681 ccgtcgatga tttattccga taaagtcatg gatcatttcc agaacccgcg gaacgtcggc
    19741 accctccccg acgccgacgg agtcggccag gtcggcaatc ccgtttgcgg cgacatgatg
    19801 accttctacg tcaaggtcaa ggacgaccgg ctgaccgaca tcaagttcca gacattcggc
    19861 tgcggagcgg ccatcgccgt ctcgagcatg gtctcggaga tggccctcgg gaaaacggtc
    19921 caggaggcga tggacatcac caacgagaaa gtagccgagg agctcggcgg cctgcccaag
    19981 aacaagatgc attgttccaa cctgggggcg gacgccctgc atgccgccct ggagaattac
    20041 caagcccgga aggccggaac ggcctccgga ccggccgccg aagcggccgc cggccgggcc
    20101 gtggaaggag cggcccccga tcactgccct tactgcgacg ggccggtgca aggtcccccg
    20161 aacatgtgtt cctgcaaaac ctgcgacctc gatctcgtcg aatgcggcca ttgccatctt
    20221 tacaccaacg gggccgaaac ccgctgcacc cactgcgggg cgcagctcga acggtaattt
    20281 catgaccgcg tcgccggcgc ctcccggccc ttcctcgtgg atccggcggg tccgggacct
    20341 ggcccgggag cgcgaggccg tcctcctcgc ccacaattac cagcggcccg aagtccagga
    20401 agccgccgat ttcgtcggcg actcgctgga gctcagccgg aaggccgccg gggtcgaagg
    20461 gcgggtgatc gtcttctgcg gcgtccggtt catggccgaa accgcggccc t
//
LOCUS       DAOOXH010000039        20146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_2542_length_20146_cov_8.133741, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000039 DAOOXH010000000
VERSION     DAOOXH010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 20146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20146
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2542_length_20146_cov_8.133741"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PKV64_06155"
     CDS             <1..274
                     /locus_tag="PKV64_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100337.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPN16698.1"
                     /translation="PGKGFAVAARSMDGKIIEAIEHERYPHVLGVQFHPEFRKLWDPA
                     PEFKIGPDDGALFSFRTLIEAHPPSFEFHKRLWSWFFETVRDGSRI"
     gene            complement(305..787)
                     /gene="rfaE2"
                     /locus_tag="PKV64_06160"
     CDS             complement(305..787)
                     /gene="rfaE2"
                     /locus_tag="PKV64_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HPN16699.1"
                     /translation="MRKIRSAAALKRLRAGWRRQGLKTVFTNGCFDLIHGGHVHLFRR
                     AKAQGDILIVAVNSDASVRRLKGPSRPVFPLAERMEVLAAFGDIDYVTWFEDDTPQKI
                     IAALLPDVLVKGGDWGAGEIVGTADVEAAGGRVYRVPYRRGHSSTNLIEKIRRSARRR
                     "
     gene            complement(789..2192)
                     /locus_tag="PKV64_06165"
     CDS             complement(789..2192)
                     /locus_tag="PKV64_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN16700.1"
                     /translation="MAAIELDRVSRIYQKYSSRHRFQTFKSAIVKGSLFGSLKPDELV
                     RAVDDVSFSVEKGQTFGLIGENGSGKSTILKMVAGIAKPSSGRISVTGKVSALIELGA
                     GFHPEISGRENVYINGIMLGLSKKEIARKFDEIVRFAELEDFIEAPVKTYSSGMYMRL
                     GFSIAINVNPEVLLIDEVLAVGDAAFVPKCLDRIDDFRRRKKTILFVSHDLSTVAKIC
                     NTVAWMKNGKIRMIDEPKRVVDAYMQDVTEAQEAEFRERKEEAAAAVVAVPAPPPAAE
                     GPASARLEQGPAAEAAVTAAPAEAPAPAAEPPAAAEEGRREQRWGKREVEIRRVVLRN
                     GQGEEKHVFRPGETMVIDMDIAAREPVKDFVFGLAVFNSMGVLCYGTNTHLEEFESVA
                     FSGEGRVVCRIESLPLINGTYYLDLAAHKRDGYPYDYHRNMYSFLVSSMIRDEGVARL
                     DHAWEFSPEIKIKSPAK"
     gene            complement(2192..3001)
                     /locus_tag="PKV64_06170"
     CDS             complement(2192..3001)
                     /locus_tag="PKV64_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN16701.1"
                     /translation="MKLIANLQEVYKHRLLIRTLVGRELKARYRGTVLGFFWSFFNPF
                     LLMVVYTVVFGFILKPRDPTLGEGPWMYALYLFAGILPWTWFSSSLNDSANVLMVNGN
                     LIKKILFPAEVLPIVSVTSNFIHFLFGLPILLIFLFVFGKPPTVYLLLLPAVLLVQFV
                     FSLGFSLLLSALTVHFRDIKDILSNLLTFWFFSTPIVYSLGMLEHSPALRAVLDLNPM
                     THIIGGYHDCLFFGQMIHWKRLGVTLIVSLVLFVIGYAVFDRLRDSFPEEV"
     gene            complement(2985..3740)
                     /locus_tag="PKV64_06175"
     CDS             complement(2985..3740)
                     /locus_tag="PKV64_06175"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPN16702.1"
                     /translation="MDFPLRRGETLDDFYRGRVRLIQPRKGYRFATDAPLLAAFAGLR
                     PGDEVCELGTGNGVIAVLLGFKPFARLTAVEIQPRLADIARRNVAGNGLSGRIEVVEA
                     DLRTFRPGKRFDVLVSNPPYHGKKTGFPCPDREKAIAKHEITCDIMELMRAVSELLKD
                     DGRAFFIYPAAREEEFRAAARSRGLRPVVIRRIHPRAGEEPNLFLSELRFAAAFSGEE
                     TVLPPLVLHPGRGGEFGPEAAAMLEGPLNETHR"
     gene            complement(3757..5040)
                     /locus_tag="PKV64_06180"
     CDS             complement(3757..5040)
                     /locus_tag="PKV64_06180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPN16703.1"
                     /translation="MSGKMDQRMGGAEPAAETAELVRSRGIQAAVNALRRRELALGLR
                     KPTLAVYDHAFHFIGGAQKYGLTMAAALRDMFDLTILANRDVGPEEFRRWYGLDLSGC
                     PVKVVPIPFYDERNLIHLDPALVGRADENPFHRLSRESGGYDVFVNNSMNEMVFPLAP
                     VSVFICHFPERRPRTYFYADRYTRVVCNSRYTAAWIEKKWGFSPHRLLYPPVDMEAEG
                     EAGPKKKLILSASRFEPEGFKRQREMITVFLKLLRERPEAADGWTFVLAGGSGPGNAY
                     LDGLRELAAGAAGRIELRVNVTAAELKSLYRDAALFWHMCGLNGEDPGQTEHFGMTTV
                     EAMQNAVVPLVFAGGGLPEIVDDGINGFLAGSTARLLERTLFLLRDPGKREALGRAAR
                     EKARRFSRPRFEAETRMIFEDVLALLAGSGDPAAE"
     gene            complement(5076..5591)
                     /locus_tag="PKV64_06185"
     CDS             complement(5076..5591)
                     /locus_tag="PKV64_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16704.1"
                     /translation="MNKLEQEIRRLREELDRKREELADIRKLFGGEIDREFSALVEGY
                     SDRELELHLQEVLASMDALAEARPDLRAVSSHRKTIGRPIVFLKRVLLETTFAQFNVF
                     LERQRQFNRRILDLLRALHVRDVRGQARFKALEARIAGCEEDLVLLRAKLEDLRAAEP
                     GSSGPEHPGRG"
     gene            complement(5578..8172)
                     /locus_tag="PKV64_06190"
     CDS             complement(5578..8172)
                     /locus_tag="PKV64_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPN16705.1"
                     /translation="MRILVVTGGYPPADRTSADLACRGLVEGLTRRGHEVRVLAGGAF
                     PGAPLGTEPVLRRLVRDRADRNAWSDVFRKELANQAALRESILGFRPAAALFFGLPRI
                     SASLPLLAESLGVASCLYVDGDSLAVWERDLWYREQPRGPRGRRAIRFLTRRFGLSAF
                     PRPLHELPAVFTSRYVLEATERVGKPVRRAAVFPPGVDLRRFPFKETAGARPVRMLFL
                     GPVTPEDGADVAVRTLGLLKRDPGGAGLTLTVAGPLSGFSEILASLQDRAEQAGAAGD
                     VSFVEYAPDMSSPDMLLGHDLFLHPAARVGSITPALLEAMACGLPVVAAARGGNSEVL
                     EDGRNALVVPPENPEACARAVRRLLDDPALREMLRRNARKTIEERFRLDRTVEAVEKI
                     LEEIAADPRPFSAVSRDEEDAAGGEDVSASGRPERWLRWSGVLIRIRRLFRPKAVAAK
                     LKGGARIAACRLSLLAFPPVLKGIHRWLGRRRSRPAGGEPREVLVVQLADLGDIVLSG
                     PFLRNLRTLLPRSKIVLAVHPGGLNIIEKCPEVDEILTFPYRSFARWNDAFYGSVRWW
                     LKGLTLAVRTFRKRRFDLAVSLRWNNDAPQAASLILMAASGAPRRVAYLNPPNAPAKP
                     GRNGLNGLITDGPARGAEKHEVEYQADLLRHLGGRSEDASLKVWTTADDEQKARNLLD
                     GHRIGSGELVIAFAPGARWEFRRWPAERFVALGRWLQDVHGARILILAAKSETALAAE
                     IERGLKPEGTVNLAGKTTIREMAAVLRSCRYFVGNDSGPMHIAVAAGVVSVGLFGPGE
                     YARFRPWGPAHAVVHLGLTCNPCSQNCLFSEARCIQGISVAQVQDIMTGLLGSPSLSG
                     KAVEDEQA"
     gene            complement(8284..8577)
                     /locus_tag="PKV64_06195"
     CDS             complement(8284..8577)
                     /locus_tag="PKV64_06195"
                     /inference="COORDINATES: protein motif:HMM:NF014838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16706.1"
                     /translation="MSDKAPDRFRLRVVTSGALLVETQADEAQIPTIDGLIGVFPLHR
                     PLVVALGRGTLSYRQGAYEERFEIDGGYAEISPDSVLVFTETAAADSSADGAE"
     gene            complement(8574..10007)
                     /gene="atpD"
                     /locus_tag="PKV64_06200"
     CDS             complement(8574..10007)
                     /gene="atpD"
                     /locus_tag="PKV64_06200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPN16707.1"
                     /translation="MNQPAPAPRAGVVVQVIGPVVDILFKGQTVPEIYSAIRITSEGF
                     DSPSPVDIVVEVEQHIGEDTVRCVAMHPTEGLARGMKAVDTGGPITVPVGPEVLGRVM
                     NVIGEPVDRLGPIETSLRYPIHRQPPTLDDQNTHLEMFETGIKVIDLIQPFLKGGKIG
                     LFGGAGVGKTVIIMELIQNVAMKHGGFSVFAGVGERTREGNDLWLEMKESKVIDKAAL
                     IYGQMTEPPGARLRVGLTALSVAEYFRDEMNQDILLFIDNIFRFTQAGAEVSALLGRM
                     PSAVGYQPNLATELGELQERITSTKKGSITSVQAIYVPADDFTDPAPATTFSHLDAST
                     SLSRALTQMGIYPAVDPLASTSRILDPRVVGDEHYRVARRVKEILQRYKDLQDIIAIL
                     GIEELSDEDKLIVARARKIQRFLSQPFHVAEEYTGREGKYVTIDETVRGFREIVEGLH
                     DDKPEQAFYMVGGIDDVVRRAEELKNA"
     gene            complement(10025..10885)
                     /gene="atpG"
                     /locus_tag="PKV64_06205"
     CDS             complement(10025..10885)
                     /gene="atpG"
                     /locus_tag="PKV64_06205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPN16708.1"
                     /translation="MANLIDLRRRIRSVRNSQQVTKAMKTVATAKYKKASRTIAEGRP
                     LWHSFPDSVGRLAGWAGPDAHPFLDVREERTIDVLVMTSDKGLCGAFNSNLLAKTLEF
                     LKSKEPEARVRLELIGRKAAQFFKRLPYPIDRAYAEHTDRIGAGELRSLAAGLMEKFL
                     FLETDAVYAAYNEFKSVLAPRLAIVRILPVDSGGGEEGGAVVPDWEPLPSPLIAAYWP
                     RYVESQIVHAYAESQAAEQAARMMAMDAASNNAEELIEVLTLNLNKARQAGITKELLE
                     IMTAVEALKS"
     gene            complement(10885..12402)
                     /gene="atpA"
                     /locus_tag="PKV64_06210"
     CDS             complement(10885..12402)
                     /gene="atpA"
                     /locus_tag="PKV64_06210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPN16709.1"
                     /translation="MAIKADEISKIIQRQIEGFEESVDIREVGTVITVGDGIARVHGL
                     DRVMYNELLEFPHDVLGLALNLEEDSVGVVLLSESSMIVEGDLVRRTHRIISVPAGPP
                     LIGRVIDPLGRPLDGKGPIKSDVFMPVERLAPGVVDRLPVREPLQTGIKAIDSMIPIG
                     RGQRELIIGDRQTGKSAIVLDTILNQKDTGVICVYVAVGQKNSTVAHFIKTLEDHGAM
                     EYSVVVVAAASDPSPLQYLAPYSGCAVGEWFRDNGRHALCVYDDLSKHAAAYREISLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSLTALPIIETQAGDVSGYIPTNV
                     ISITDGQIYLEAELFNSGIRPGINVGISVSRVGGHAQTKAMKQVAGKLRGDLQQYREL
                     LSFAQFGTELDRISQAQLDRGIRLTEILKQSQFEPLPIEKQVVILYAGNRGYLDEIPV
                     GKIRDYEKKLYAFIEREHPEIFAGIADKKALDKHLDEAIAAALEEFGRRFREEAA"
     gene            complement(12392..12946)
                     /gene="atpH"
                     /locus_tag="PKV64_06215"
     CDS             complement(12392..12946)
                     /gene="atpH"
                     /locus_tag="PKV64_06215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HPN16710.1"
                     /translation="MNPLLIKRYTEGLAGALPDESEYRVVSKDLEDFLGLVKGHERLF
                     ALLSRPFLRPTKKAAVIKEILARVDYHDKSKRFLLLLVRQRRLDLLPEIIRNLPAKWK
                     ELRGIRTFDVRSVVLLSEKQKSRLEAELARLEQAPVSCSYGLDPALIGGLFIRKGNRV
                     YDASLRGELQRLKDIIGGREPHGH"
     gene            complement(12927..13514)
                     /locus_tag="PKV64_06220"
     CDS             complement(12927..13514)
                     /locus_tag="PKV64_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16711.1"
                     /translation="MNVSRALAVLLLVSFPAAGFAAGEAEHAAGGGGLDFLWKVINFA
                     VLFGALFYFLRKPLQAMLDKKAETVRDLLADARREREEAEAKLTEAATRVAELQDEAA
                     RLKERAAAEARAETERIRDIASREAERIRTLAAQEVAVRLQAGIRELKEYTTELAAGL
                     AEERLKKRLTEADHIDLIDRSIDRLKVMSDESAAH"
     gene            complement(13511..13933)
                     /locus_tag="PKV64_06225"
     CDS             complement(13511..13933)
                     /locus_tag="PKV64_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HPN16712.1"
                     /translation="MLEIDASVIVTFVLVWILVVVLNRVFFKPLRGVMDAREKRLQDD
                     MAAAQSNLDETARRLKAVDAGLKAARLEAEETRDRVELEALREKNRLITEAGQAAKTE
                     IENARAAFEAEVARLKEELRAEAVPLSEKIEEKLLHRS"
     gene            complement(14018..15346)
                     /locus_tag="PKV64_06230"
     CDS             complement(14018..15346)
                     /locus_tag="PKV64_06230"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPN16713.1"
                     /translation="MNTPRSAAPSGKGLSPAACRAYLADLERFGIKLGLDNMRALLRG
                     LGDPHEAYPLIHVAGTNGKGSVAAMLAAILARSGRRIGLYTSPHLVRVEERIRINGRP
                     VSEGDFRLRIAVIRAKIQELRAARILDVSPTYFEVLTAAALLHFREKRVDGAVLEVGM
                     GGRFDATNVVRPLVSIITTVSRDHMEYLGNTLAKIAFEKAGITKPGVPVVSGVAPGPA
                     LAVIRRRAEEEKAPLIEVFGRGASFESRFRGGGRFLYRAGGNVFRLAPSLTGEHQGRN
                     AAVAAAAARVLDKVWSPVGIREIEEGIRDTRWEGRLERAGTRPRVFLDGAHNEAGAEA
                     VAAFIKSAASRRPVLVFAVMKDKAVGRIARILFPAVRRIILTTIPYKRAASPEEILAK
                     APAFASKTVLEPDLGKALAQARRLAGPGGTVFVAGSLFLVGEVKKILRSR"
     gene            complement(15343..16134)
                     /gene="accD"
                     /locus_tag="PKV64_06235"
     CDS             complement(15343..16134)
                     /gene="accD"
                     /locus_tag="PKV64_06235"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HPN16714.1"
                     /translation="MTNLWTRCNNCQRILYKEDILDNLSVCPACAFHFRLSASEWLAL
                     LYDDARFDITDHDILPRDPLHFVDSQPYHKRLADNQRKTSLPEAVVNGLGMIGGIRTA
                     ISALDFAFMGGSMGSVVGEKITRAAERAMAEKIPFIIISCSGGARMQEGALSLMQMAK
                     TCAALGRLEQAGLPFISVLADPTTGGVTASFAMLGDLNIAEPNALIGFAGPRVIEQTI
                     KEKLPKGFQRSEFLLSHGMIDDVVPRKNLKAYLVRALRLFLNKSA"
     gene            complement(16172..16636)
                     /locus_tag="PKV64_06240"
     CDS             complement(16172..16636)
                     /locus_tag="PKV64_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN16715.1"
                     /translation="MADKKILLVDYDTKNRDAVAALFEPYRFQIFQASDGAAAYDSFK
                     IENPDLVILEAILPKLHGFDLTKRITQESKGRTSVIIVTGLYRGPQYKQEALTSFGAA
                     AYFEKPFDGEHLLQTALDLLREKSDFGVDLPTAAEVIRLLSDRLKAAPPARG"
     gene            16735..17709
                     /locus_tag="PKV64_06245"
     CDS             16735..17709
                     /locus_tag="PKV64_06245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPN16716.1"
                     /translation="MAKGIDLSIIVPVFNEEKNLRPLHAEIKAAGDKLGRTYEIILVD
                     DGSRDGSWDVLRSIQASDVRVRAIRLRKNFGQTAALSAGFDNARGRIIVTLDADLEND
                     PADIGMLIDKIEEGYDLVSGWRKDRWKKHFFTRRIPSLLANRLISAITKVRLRDFGCT
                     LKAFRREVIENIHLYGEMHRFIAAIAASVGVAIAEVPVNFRPRIHGKSKYGISRSIRV
                     MLDLLTVKFLLSYSTRPLQIFGLMGLISGAAGTVIGLILSYQRLVLKTAIGNRPLLLL
                     AVLLLVVGFQFVTMGLLGEIMVRTYHEAVEKHIYVIREVIETPPTKAE"
     gene            17720..18886
                     /locus_tag="PKV64_06250"
     CDS             17720..18886
                     /locus_tag="PKV64_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPN16717.1"
                     /translation="MKILMLAPEPFFQPRGTPISVYFRLKALSDLGHSVDLVTYPRGG
                     DVAFPGLAIKRVPNLIGVRDIKIGPSLVKLPLDALMFATAFGSLLRKRYDLIFSHEEA
                     ALLGVLLGKIFRTPHLYDMHSSLPQQLENFDFSRSALLKTAFLRMERFILKGSRSVIV
                     ICRDLLDYAARLGFGGKAVFLENILDFNDFDPAPPGPVEIAAVRRDIAGDGEKIVLYA
                     GNFEPYQGVSLLVEAFGRMKERAVLLIVGGSKADHEPARKRAAELGFADRFVLVEKVP
                     PQRVPVYISLSDVLVSPRVSGTNTPLKIYAYLKAGKPVVATDLWTNSQVLTEEIAVLA
                     PPDPEKFAAALDFALTSAEAERRARAGADLAAREYTYPRYKEKITEAVTRAVRR"
     gene            18883..19851
                     /locus_tag="PKV64_06255"
     CDS             18883..19851
                     /locus_tag="PKV64_06255"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPN16718.1"
                     /translation="MNAFVTGGTGFVGNHLCAALAASGWTVKALGRRLRNNPPPGVEF
                     VSGDIRDAALLDRASAGADVVFHLASALGAARIGAAEFMAVNAGGTRAVLAAARENGV
                     RRVVHFSSAGVLGHVRPGETADEKYPLDPRNVYDRTKLAGERAALEAASAGQDVVVIR
                     PGWIYGPGDRRTFKLIRSIAGGRFLLPGRGETLQSPAYIDDLVAGTLLCAGRGRAGEI
                     YNIAGNQAMPVRKMVETIAAALGKSLPRFHPPMGLTRAAGAVLGILFGFAGKEAPLNP
                     SRLAFFVHPKGLNIDKARRELGFEPAVGFAEGMARAVDWYRRAGWL"
BASE COUNT         3587 a         6518 c         6495 g         3546 t
ORIGIN      
        1 gcccggcaag ggcttcgccg tcgccgcccg ctcgatggac gggaaaatca tcgaggcgat
       61 cgagcacgag cgatatcccc atgtgctcgg cgtccagttc cacccggaat tccggaagct
      121 ctgggacccg gcgccggagt tcaagatcgg gccggacgac ggggcgctgt tttcgttccg
      181 gacgctgatc gaagcccatc ccccaagctt cgagttccac aagaggctgt ggtcctggtt
      241 tttcgagacg gtccgggacg gctcccggat atagggagag cttcttttcc aggccgttcc
      301 gccgtcagcg gcggcgggcc gatctccgga ttttttcgat gaggttcgtg ctggagtggc
      361 cgcggcggta tgggacgcgg tacacccgtc ccccggccgc ctcgacgtcc gccgttccga
      421 cgatttcgcc cgcgccccaa tctccgccct tgaccaggac gtcgggcagc agggcggcga
      481 tgatcttttg gggcgtatcg tcctcaaacc aagtcacata gtcgatatct ccgaacgcgg
      541 ccaggacctc catccgctcg gccagcggaa acaccggccg gctcgggccc ttcagcctcc
      601 ggaccgaggc gtcggaattg acggccacga tcaggatgtc gccctgggcc ttggcccggc
      661 ggaagagatg gacgtgcccg ccgtggatca gatcaaagca gccgttggtg aagaccgtct
      721 ttaagccttg gcggcgccag ccggcccgca ggcgcttcaa ggcggccgcc gaacgaatct
      781 ttctcatctc atttcgccgg cgatttgatc ttgatctccg gggagaattc ccaggcatgg
      841 tccaagcggg ccacgccctc gtcccggatc atcgaggaaa cgaggaagga atacatgttc
      901 cggtggtaat catatgggta tccgtcgcgc ttatgagcgg ccaggtcgag gtaataggtc
      961 ccgttgatca ggggaagcga ctcgatccgg cagacgaccc ggccctcccc cgaaaaagcg
     1021 acggactcga actcctccag gtgggtgttc gtcccgtagc acaacacccc catggagttg
     1081 aaaacagcga ggccgaacac gaaatccttg accggctcgc gggcggcgat gtccatatcg
     1141 atcaccatcg tctcgccggg acggaagacg tgcttttcct ccccctggcc gttccgaaga
     1201 acgacccgcc ggatctccac ttcccgcttg ccccagcgct gttcgcggcg tccttcctcg
     1261 gcggcggccg gcggttcggc cgcgggggcg ggagcttcgg ccggagccgc ggtcacggcg
     1321 gcttccgccg ccgggccttg ctccaagcgg gcggaggccg gcccttccgc ggccggcggg
     1381 ggggccggaa cggcgacgac ggccgcggcc gcttcctcct tccgctcgcg gaactcggcc
     1441 tcctgcgcct cggtgacatc ctgcatataa gcgtcgacga ccctcttggg ctcgtcgatc
     1501 atccggatct ttccgttctt catccaggcc accgtgttgc agatcttggc cacggtggac
     1561 aggtcgtggc tgacgaacag gatcgtcttt ttccggcggc ggaaatcgtc gatccggtcg
     1621 agacacttgg gaacgaaggc cgcgtcgccg acggccagga cctcgtcgat gagcaggacc
     1681 tcgggattga cgttgatggc gatggaaaat cccagccgca tgtacatccc cgaggaatac
     1741 gtcttgaccg gagcctcgat gaaatcctcg agctcggcga agcggacgat ctcgtcgaac
     1801 ttgcgggcga tctccttctt ggacagcccg agcatgatgc cgttgatata cacgttttcc
     1861 cggccggaga tttccggatg gaacccggcg cccagctcga tcagggccga aaccttgccc
     1921 gtcaccgata tccgcccgga ggagggcttg gcgatcccgg cgaccatctt caggatcgtg
     1981 ctcttgcccg agccgttctc cccgatcaga ccgaaggttt gccctttctc gacgctgaag
     2041 ctgacgtcgt cgacggcccg gacgagctcg tccggcttga gcgacccgaa caggcttccc
     2101 ttgacgatgg cgctcttgaa cgtctggaac cggtgccggg aggagtattt ctggtagatc
     2161 cggctgacgc ggtccaactc gatggcggcc atcagacttc ctccgggaag gagtcgcgga
     2221 ggcggtcgaa gacggcgtag ccgatcacga acaggaccag ggagacgatc aacgtgactc
     2281 ccagccgctt ccagtggatc atttgcccga agaagaggca gtcgtggtag cccccgatga
     2341 tatgggtcat cgggttgaga tcgaggacgg cccggagggc cggcgagtgc tccagcatcc
     2401 cgagagaata gacgatcggc gtggagaaga accagaacgt cagcaagttg ctgaggatgt
     2461 ccttgatgtc ccggaagtga acggtcaggg cggacaacag gaggctgaag cccagggaaa
     2521 agacgaactg gaccaggagg acggccggca gcaggaggag gtagaccgtc ggcggcttgc
     2581 cgaaaacaaa caggaagatg agcaggatcg gcaggccgaa gaggaagtgg atgaagttcg
     2641 aggtgaccga gacgatgggg aggacctcgg ccgggaagag gatcttcttg atgaggttgc
     2701 cgttgaccat caggacgttg gccgaatcgt tcaacgatga cgaaaaccac gtccagggca
     2761 ggatcccggc gaacaagtac agggcataca tccagggccc ctcgccgaga gtaggatcgc
     2821 gcggcttgag gatgaatccg aaaacgacgg tatagacgac catcaggagg aacgggttga
     2881 agaaagacca gaagaagccc aggaccgtcc cccggtagcg ggccttcagc tcccgtccga
     2941 ccagcgtccg gatgagaagc cggtgcttgt aaacctcctg gaggttagcg atgagtttca
     3001 ttcaacggtc cctcgagcat cgccgccgct tccggtccga attctccgcc ccggccggga
     3061 tggaggacga gcggcgggag cacggtctcc tcccccgaaa aagccgcggc gaaccggagc
     3121 tcggacagaa aaaggttcgg ctcttcgccg gcgcgcggat gaatccgccg gattacaacc
     3181 ggccgcaggc cgcggctccg ggccgcggcc cggaattcct cctcgcgggc ggccgggtaa
     3241 ataaaaaaag cccgtccgtc gtctttcagg agctcggaaa ccgcgcgcat gagctccata
     3301 atatcgcacg ttatttcatg ttttgcaatc gccttctccc tgtccgggca gggaaagccg
     3361 gtctttttcc catgatacgg gggattggaa accagcacgt cgaaccgctt cccgggccgg
     3421 aaggtccgca ggtcggcctc gacgacctcg atcctcccgg acaggccgtt tccggcgacg
     3481 ttccggcggg ctatgtcggc cagccgaggc tggatctcca ccgccgtgag gcgggcaaac
     3541 ggcttaaaac ccagcaagac ggcgatgacg ccgtttcccg tccccagctc gcagacctcg
     3601 tcccccggcc ggaggccggc gaacgccgcc aagagcggag catccgtggc gaaccggtat
     3661 cctttcctcg gctggatcag gcggacgcgg ccgcggtaga aatcatccag cgtttcgccg
     3721 cgtcgcaagg ggaaatccat cgccgctctc tccgtctcat tccgcggccg gatcgccgga
     3781 gccggcgagc agcgcgagga cgtcctcgaa aatcatccgg gtttccgcct caaagcgcgg
     3841 acgggaaaac ctccgggctt tctcccgggc ggcccgcccc aaggcttctc tttttcccgg
     3901 atcccggaga aggaacaacg tccgctccaa gaggcgggcc gtggagccgg ccaggaatcc
     3961 gttgattcca tcatcgacga tctccggcag cccgcctccg gcaaaaacga gcgggacgac
     4021 ggcgttctgc atggcttcca ccgtggtcat cccgaaatgc tccgtttggc cgggatcctc
     4081 gccgttcagg ccgcacatgt gccagaacag ggccgcgtcc cggtaaaggg atttcaactc
     4141 ggccgcggtg acgttgaccc ggagctcgat ccggccggcc gctccggcgg ccaactccct
     4201 caagccgtcc aaataggcgt tcccgggccc gcttcccccg gccaggacga acgtccagcc
     4261 gtccgccgcc tccggccttt cccggagaag cttcaagaaa accgtgatca tctccctctg
     4321 cctcttgaat ccctccggtt cgaagcggga ggcggaaagg atcagcttct tcttgggacc
     4381 ggcttcccct tcggcctcca tgtcgacggg cggatacaga agccggtggg gcgaaaaccc
     4441 ccattttttt tcgatccagg cggccgtgta gcggctgttg cagaccaccc gggtataccg
     4501 gtcggcgtaa aaataagtcc gcggccgccg ttccgggaaa tggcagatga acacggagac
     4561 gggagccagg ggaaagacca tctcgttcat gctgttgttg acgaagacgt catagccgcc
     4621 gctttcccgg ctgaggcggt ggaacggatt ttcatcggcc cgtccgacca aggccgggtc
     4681 gagatggatc aagttccgct cgtcgtagaa gggaatcggg accactttga ccgggcagcc
     4741 ggagaggtcc aagccgtacc atcgccggaa ttcctccggc ccgacatcgc ggttggccag
     4801 gatcgtgagg tcgaacatgt cccggagggc ggcggccatg gtcagcccgt atttctgggc
     4861 cccgccgatg aaatgaaagg catgatcata gacggccagg gtcggtttcc ggaggccgag
     4921 agccagttcc cgcctccgaa gggcgttgac ggcggcctgg attccccggc ttcggacgag
     4981 ttcggccgtt tcggcggccg gttcggcccc gcccatccgc tgatccattt ttccgctcat
     5041 ctcccttccg tccgtgtcct tccgaaagcc ggcgttcagc cccgccccgg atgttcggga
     5101 ccgctcgaac ccggctccgc cgcccgcaag tcctcgagct tcgcccggag aaggaccagg
     5161 tcctcctcgc agccggcgat tcgcgcttcc aaggctttaa accgcgcctg cccccggacg
     5221 tcccgcacat gcagggcccg caacaggtcc aagatccgcc ggttgaactg gcgctgtcgc
     5281 tccaggaaga cattgaactg ggcgaacgtc gtttccagaa ggacccgttt caagaaaacg
     5341 atcggccggc cgatggtttt ccgatgggag gagaccgccc ggagatccgg ccgggcctcg
     5401 gccagggcgt ccatcgaggc cagaacctcc tggagatgga gctcgagctc ccggtccgag
     5461 tatccttcca cgagagcgga aaactcccgg tcgatttctc cgccgaagag cttccggatg
     5521 tcggccagct cctcccgctt gcggtccaac tcctccctca gccgccggat ttcctgctca
     5581 agcttgttca tcttcgaccg cctttccaga aagagatggg gagcccagca ggcccgtcat
     5641 gatatcctga acctgggcga cggaaatgcc ctggatgcag cgcgcctcgg aaaacaggca
     5701 gttttgcgag cagggattgc acgtcaaacc caggtggacg accgcgtggg ccggccccca
     5761 aggccggaag cgggcgtatt cacccggccc gaacaggccg acggaaacga cccccgcggc
     5821 gaccgcgata tgcatcgggc cggagtcgtt gccgacgaag taccggcagg atctcaggac
     5881 ggcggccatc tcccggatcg tcgtcttccc ggccaggttc accgtcccct ccggcttcaa
     5941 tccgcgctcg atctccgcgg ccagcgccgt ttcggatttc gcggccagga tcaggatccg
     6001 ggcgccatgg acgtcctgga gccaccgccc gagggcgacg aaccgctccg ccggccagcg
     6061 gcggaattcc cagcgcgcgc cgggagcgaa ggcgatcacg agctccccgg agccgatccg
     6121 atgcccatcc aggaggttcc gggctttttg ttcatcgtcg gcggtcgtcc aaaccttcaa
     6181 ggacgcgtcc tcggaccgtc ctccgagatg acggaggaga tccgcctggt actcgacctc
     6241 atgcttctcg gcgccccgcg ccgggccgtc ggtgatcagg ccgttgaggc cgtttcttcc
     6301 cggtttggcc ggggcgttcg gcggattgag gtaagccacc cgcctcgggg cgccgctggc
     6361 ggccatcagg atcagggaag ccgcctgggg ggcgtcgttg ttccagcgca gggaaaccgc
     6421 caagtcgaag cggcgcttcc ggaacgtccg gacggccagg gtcaatcctt tcaaccacca
     6481 ccggacgctt ccgtaaaagg cgtcgttcca acgagcgaag ctccggtagg gaaaagtcag
     6541 gatctcgtcg acctcgggac atttttcgat gatattcagg ccgccgggat ggacggccag
     6601 gacgatcttc gaccggggca ggagcgtccg caggttccgc agaaacggcc cggacaagac
     6661 gatatcgccg aggtcggcca attggacgac gagcacttcc cgcggctcgc cgccggccgg
     6721 ccgggagcgc ctccggccga gccagcgatg gattcccttg aggaccggag gaaacgcgag
     6781 gagagacagc cggcaggcgg cgatccgcgc cccgcctttc agcttggcgg ccaccgcctt
     6841 cggccgaaac aggcgccgga tccgaatgag tacgccgctc caacggagcc agcgctcggg
     6901 gcggccggac gcggacacgt cctcgccgcc ggccgcgtcc tcctcgtccc gggatacggc
     6961 cgaaaacggc cggggatccg cggcgatctc ctccaggatc ttttcgaccg cttccacggt
     7021 ccggtcgagc ctgaaccgct cctcgatcgt tttccgggcg ttccgccgca gcatttcccg
     7081 gagggccgga tcgtccagca gccgccggac ggcccgggcg caggcttccg gattttccgg
     7141 aggaacgacc agggcgttgc ggccgtcctc aaggacctcg gaattcccgc cccgggcggc
     7201 ggccacgacc ggaaggccgc aggccatcgc ttccagcaag gccggggtga tggatcccac
     7261 tcgggccgcg ggatggagga acaggtcgtg gccgagaagc atgtccggcg acgacatgtc
     7321 cggggcatac tcgacaaagg aaacatcgcc ggccgcgccg gcttgctccg cccggtcctg
     7381 cagggacgcg aggatctcgg agaaaccgga aagcggcccg gcgaccgtca gggtcaagcc
     7441 agccccgcct ggatctctct tcagcaagcc cagggtccgg acggcgacat cggcgccgtc
     7501 ttcgggcgtc accggcccga gaaaaagcat gcggaccggc cgcgccccgg ccgtttcctt
     7561 gaaaggaaaa cggcggagat cgacgcccgg cgggaagacg gccgcccggc ggaccggctt
     7621 gccgacgcgt tccgtcgcct ccaggacata ccggctggtg aagacggccg ggagctcgtg
     7681 gagaggacgg gggaaagcgg aaaggccgaa gcggcgggtg agaaagcgga tcgccctccg
     7741 cccgcgcggt cctcggggct gctcccggta ccagaggtcc cgttcccaaa cggccaggct
     7801 gtcgccgtcg acatagaggc aggaggcgac tcctaagctt tcagccagaa gcggcagcga
     7861 agccgaaatc cggggcaaac cgaagaagag cgccgccgcg ggccggaatc cgaggatgga
     7921 ttcccgaagg gcggcctggt tggccagctc cttccggaaa acgtcggacc aggcgttccg
     7981 gtcggcccgg tcgcggacaa gccggcgaag gacgggctcc gtcccaaggg gcgcgcccgg
     8041 aaacgccccg ccggccagta cgcggacttc gtgtcccctc cgggtcagac cctcgacaag
     8101 gccgcggcag gcgagatccg cggaagtccg gtccgcggga ggatagccgc cggtcacgac
     8161 gaggatgcgc atgcgtcttc tctctttatc tctttttttt caaatatata tcatcattgg
     8221 ataaatcttg gcaaaccggg cgcgggcggg ggattccagg gaacgaaacg cggaatccgg
     8281 taattattcc gccccgtcgg ccgaggaatc ggcggccgcc gtttcggtga agaccaggac
     8341 ggaatccggg ctgatttcgg cgtacccgcc gtcgatttcg aaccgctcct cataagcgcc
     8401 ttgacggtaa gacagcgtgc cccgccccag ggcgacgaca aggggccgat ggaggggaaa
     8461 gacgccgatc aggccgtcga tggtcggaat ctgggcctcg tcggcctgcg tctcgaccaa
     8521 gagcgctccc gaagtgacga cccggaggcg aaagcggtcc ggcgctttat cgctcacgcg
     8581 tttttcagct cctcggcccg gcggacgacg tcatcgatcc ccccgaccat gtaaaaagct
     8641 tgctcgggct tgtcgtcgtg gaggccctcg acgatctcgc ggaagccgcg gacggtctcg
     8701 tcgatcgtca catatttgcc ttcccggccg gtgtattcct cggcgacatg gaagggctgc
     8761 gagaggaacc tctggatctt gcgggcccgg gcgacgatca gcttgtcctc gtcggacagc
     8821 tcctcgatcc cgaggatggc gataatgtcc tgaaggtcct tgtagcgctg gaggatctcc
     8881 ttgaccctcc gggcgacgcg gtaatgctcg tcgccgacga cgcggggatc gaggatgcgg
     8941 ctggtcgagg ccaggggatc gaccgccggg tagatcccca tctgggtcaa ggcccggctc
     9001 aaactggtcg aggcgtcgag gtgggagaac gtcgtggccg gggccggatc ggtgaaatcg
     9061 tcggccggga catagatcgc ttggaccgag gtgatcgatc ctttcttcgt cgaagtgatc
     9121 cgttcctgca gctcgcccag ctcggtggcc aggttcggct ggtagccgac ggccgagggc
     9181 atccgtccga gcagggccga gacctccgct ccggcctggg tgaagcggaa gatgttgtcg
     9241 atgaagagga ggatgtcctg gttcatctca tcgcgaaaat actcggctac cgagagggcc
     9301 gtcaggccga cccggagccg ggcgcccggc ggctcggtca tctgcccgta gatcagggcg
     9361 gccttgtcga tgaccttgct ttccttcatt tccagccaca gatcgttgcc ctcgcgcgtc
     9421 ctctccccga ctccggcgaa aaccgaaaag ccgccgtgct tcatggccac gttttggatg
     9481 agctccatga tgatgacggt cttgccgacg cccgctccgc cgaacaggcc gatcttgccg
     9541 cccttaagga acggctggat gaggtcgatg accttgatcc ccgtctcgaa catctccaag
     9601 tgggtgtttt ggtcgtccag ggtggggggt tggcggtgga tggggtaccg gagggacgtc
     9661 tcgatcgggc cgaggcggtc gaccggctcg ccgatgacgt tcatgacccg gcccaggact
     9721 tcgggcccga cagggaccgt gatcgggccg cccgtgtcga ccgctttcat cccccgggcc
     9781 aggccttcgg tcgggtgcat ggccacgcag cggacggtgt cctcgccgat gtgctgttcg
     9841 acctcgacga cgatgtcgac cggcgagggc gaatcgaacc cctcgctcgt gatccggatg
     9901 gcggaataaa tttcaggaac ggtctggccc ttgaacaaaa tatccacgac gggaccgatg
     9961 acttggacga cgacgccggc gcggggcgcg ggtgcgggtt gattcatggc gttctccgtt
    10021 ggattcagga cttgagcgcc tccacggcgg tcatgatttc gagaagctct ttggtgatgc
    10081 ccgcctggcg ggctttattc aggttgaggg tcaggacctc gatcagctcc tcggcgttgt
    10141 tggacgcggc gtccatggcc atcatccggg cagcctgctc ggccgcctgg gactcggcgt
    10201 aggcatggac gatttgggat tcgacgtatc ggggccagta ggcggcgatg agcggagatg
    10261 gaagaggctc ccaatccggg acgacggccc cgccctcctc tccgcccccg gaatcaacgg
    10321 gcaggatccg gacgatggcg agccgcgggg cgagaacgga cttgaattcg ttgtaggccg
    10381 cgtagacggc gtccgtttcc aggaagagga atttttccat caacccggcg gccaaagacc
    10441 tcagctcccc cgctccgatc cgatccgtat gctcggcata agcccggtcg atgggatagg
    10501 ggaggcgctt gaaaaactgg gccgctttcc gtccgatcag ctcgagccgg acacgggctt
    10561 ccggctcctt gcttttgagg aattcgaggg ttttcgccaa aagattggag ttgaaggcgc
    10621 cgcacagccc cttgtccgaa gtcatgacga ggacatcgat cgtccgttcc tcccggacgt
    10681 ccaggaacgg gtgggcgtcc ggcccggccc agcccgccag ccgcccgacg gaatcaggga
    10741 aggagtgcca aagcggccgg ccttcagcga ttgtccggga ggctttcttg tacttggccg
    10801 tggcgaccgt cttcatcgcc ttcgtaacct gctgggagtt gcggacgctc cggatgcgcc
    10861 gccgcaggtc gatcaggttg gccatcaggc ggcctcctcg cggaagcgcc ggccgaattc
    10921 ctccaaggcg gcggcgatcg cctcgtccaa gtgcttgtcg agggcctttt tatcggcgat
    10981 cccggcgaaa atctccgggt gctcccgctc gatgaaggca tagagcttct tttcataatc
    11041 gcggatcttg ccgacgggga tctcgtccaa gtacccgcgg ttgccggcat aaaggatgac
    11101 cacctgcttt tcgatcggga ggggctcgaa ctggctctgc ttgagaattt ccgtcagccg
    11161 gatcccccga tcgagctggg cctgggaaat gcggtcgagc tccgtgccga actgggcgaa
    11221 cgagaggagc tcgcggtatt gctggagatc gccgcggagc ttgccggcga cctgtttcat
    11281 ggccttggtc tgggcgtggc ccccgacccg ggagaccgag atgccgacgt tgatcccggg
    11341 ccggatgccg gaattgaaaa gctcggcctc gaggtagatc tggccgtcgg tgatcgagat
    11401 cacgttggtc gggatgtagc cggagacgtc gcccgcctgg gtttcgatga tcggaagggc
    11461 ggtcagcgac ccgccgccga gatcgtcatt caacttggcc gcccgctcga gcagccgcga
    11521 atggagatag aacacgtcgc cgggataggc ctcgcgtccg ggcggccggc ggaggagaag
    11581 ggagatctcg cggtaagccg cggcgtgctt ggacaggtcg tcatagacgc agagggcgtg
    11641 gcggccgttg tcccggaacc actcgccgac ggcgcatccg ctgtagggag cgaggtattg
    11701 gagaggcgac ggatcggagg cggcggccac gacgaccacg gaatattcca tcgctccgtg
    11761 atcctccagg gttttgatga agtgggcgac cgtggaattc ttctggccga cggcgacata
    11821 gacgcagatg acgccggtgt ccttctggtt gagaatcgtg tccaggacga tggccgactt
    11881 tccggtctgg cggtcgccga tgatcagctc gcgctggccg cggccgatcg ggatcatgga
    11941 gtcgatcgcc ttgatcccgg tctggagcgg ctcccgaacc gggagccggt cgaccacgcc
    12001 cggagcaagg cgttcgacgg gcatgaagac gtcgcttttg atcggcccct tgccgtcaag
    12061 cggccggccg agagggtcga tgacccggcc gatcaggggc gggccggcgg gcacggagat
    12121 gatccggtgg gtccgccgga cgaggtcgcc ctcgacgatc atggagcttt cgctgaggag
    12181 gacgacgccg acgctgtcct cctcgaggtt cagggcgagg ccgaggacgt cgtgagggaa
    12241 ttccagaagc tcgttgtaca tgacccggtc caagccgtgg acccgggcga tgccgtcgcc
    12301 cacggtgatg acggtcccga cttcgcggat gtcgaccgac tcctcgaagc cctcgatctg
    12361 acgctggatg atcttgctga tttcgtccgc tttaatggcc atggggctcc cttccgccga
    12421 tgatgtcttt aagccgctgg agctcgcccc gcaacgaggc gtcataaacc cggtttccct
    12481 tgcggatgaa caagccgccg atgagagccg ggtcgagtcc ataggaacag gagaccgggg
    12541 cctgctccag gcgggcgagc tccgcctcca gccggctctt ttgtttctcg gacaacagca
    12601 cgaccgaccg gacgtcgaac gtccggattc cccggagctc cttccacttg gccggcaggt
    12661 tacggatgat ctcgggcagg aggtcaagcc gccgctgccg gacgagaagg agaagaaacc
    12721 gcttcgactt gtcgtgataa tcgacccggg cgaggatttc cttgatgaca gccgctttct
    12781 ttgtgggacg caggaacgga cggctcagca gggcgaacag ccgctcgtgg cccttcacga
    12841 gcccgaggaa atcctccaag tccttcgaca caacccggta ctcggattca tccgggagag
    12901 ctccggccaa gccctccgta taccgtttaa tgagcagcgg attcatcgga catcaccttc
    12961 agccggtcga tggaccggtc gatcaagtcg atgtgatcgg cttcggtcag ccgttttttg
    13021 agccgttcct cggccaggcc ggcggccagc tcggtcgtgt attccttgag ctcgcggatg
    13081 ccggcctgaa gccggacggc cacttcctgg gcggccagcg tccggatccg ctccgcttcc
    13141 cgcgaggcga tatcccggat ccgctccgtc tcggcccggg cctcggccgc ggcccgttcc
    13201 ttcagccggg cggcctcgtc ttggagctcg gcgaccctgg ttgcggcctc ggtcagcttg
    13261 gcttccgcct cctcccgctc gcgccgggcg tcggccagca ggtcgcggac cgtttcggcc
    13321 ttcttgtcca gcatcgcctg gaggggcttc cgcagaaaat agaacaacgc cccgaacagg
    13381 acggcgaagt tgatgacttt ccagagaaaa tccaatccgc cgccgccggc cgcgtgctcc
    13441 gcctccccgg cggcgaaacc ggccgccgga aaggagacca gcaggagaac ggcgagggca
    13501 cgagagacat tcatgaccga tgcagcagct tttcctcgat tttttccgaa agggggacgg
    13561 cctcggcccg cagttcctct ttcagccggg cgacctcggc ctcgaacgcg gcccgggcgt
    13621 tttcgatctc ggtcttggcc gcttgtcccg cttccgtgat caaccggttc ttttcccgca
    13681 gcgcctcgag ctcgacccga tctcgcgttt cctcggcctc caagcgggcc gccttgaggc
    13741 cggcatcgac cgccttcaac cggcgggccg tctcgtccag gttcgactgg gcggcggcca
    13801 tgtcgtcttg gaggcgtttt tcccgcgcgt ccatcacccc ccggagcggc ttgaaaaaaa
    13861 cccggttcag gacgacaaca agaatccaga ccaggacgaa ggtgacaatg acactcgcgt
    13921 cgatctcaag catgaaaggc cgttccgttc ggttatcgga aatccgagtt tatcatatcg
    13981 attcgttaaa aatccaattc cgggcgggaa cggaggctca gcgcgaacgg aggatttttt
    14041 tgacttcccc gaccaaaaaa agactccccg caacgaagac cgtcccgccc gggccggcca
    14101 ggcggcgggc ctgggccagg gcctttccca ggtcgggttc aagaacggtt ttcgaagcaa
    14161 acgccggcgc cttggccagg atttcctccg gcgaggccgc ccgcttgtag gggatcgtcg
    14221 tcaggatgat ccggcggacg gcggggaaga ggatccgggc gatccggccg accgccttgt
    14281 ctttcatgac cgcgaaaacc aaaacgggcc ggcgggacgc cgccgacttg atgaacgcgg
    14341 ccacggcctc ggccccggct tcgttgtggg ccccgtccag gaagacgcgc ggcctcgttc
    14401 cggcccgctc gagccggcct tcccagcggg tgtcccggat tccctcctcg atttcccgga
    14461 ttccaaccgg agaccagacc ttgtccagga cgcgggcggc ggccgcggcc acggccgcgt
    14521 tgcggccctg gtgttccccg gtcagggaag gcgcgaggcg gaagacgttc ccgcccgccc
    14581 ggtagaggaa ccttcctccg ccgcggaacc gcgactcgaa cgaggcgccg cggccgaaga
    14641 cctcgatcaa gggggctttc tcctcctccg cccggcgccg gatcacggcc agggccgggc
    14701 cgggggcgac gccgctgacc acgggaacgc cgggcttggt gatcccggcc ttttcgaagg
    14761 cgattttcgc cagggtgttt cccaggtatt ccatgtggtc ccgggacacg gtagtgatga
    14821 tcgagacgag aggccggacg acgttggtcg cgtcgaaccg cccgcccatc ccgacctcta
    14881 aaaccgcgcc gtcgacgcgc ttctcccgga aatggagcaa ggccgcggcc gtcaggacct
    14941 caaaataagt cggcgagacg tcgaggattc gggccgcccg gagctcctgg atttttgccc
    15001 ggatgaccgc gatccggaga cggaagtccc cttcggaaac cggccggccg ttgattcgga
    15061 tccgctcctc gacccggaca aggtgggggg atgtgtacag accgatccgg cggccggacc
    15121 gggccaggat cgcagccagc atggccgcga ccgatccttt tccgttggtt ccggcaacgt
    15181 gaatcagggg ataagcctcg tgaggatcgc ccagccccct cagcagggcc cgcatgttgt
    15241 ccaggccgag cttgatgccg aaccgctcca agtcggctaa atacgcccgg caggcggccg
    15301 gagagaggcc cttcccggaa ggcgcggcgg accgcggcgt gttcacgcgg atttgttgag
    15361 gaaaagacgg agagcgcgga ccaggtacgc tttaaggttt ttccgcggaa cgacgtcgtc
    15421 gatcatgccg tggctgagga gaaactcgga tcgctggaat cccttcggca gcttctcctt
    15481 gatggtctgc tcgatgacgc gggggccggc gaaaccgata agggcgttgg gctcggcgat
    15541 gttcagatcg cccagcatgg cgaagctggc cgtgaccccg cccgtcgtgg gatcggccag
    15601 gacggagatg aacggcaggc cggcttgttc caggcggccg agggccgcgc acgtcttggc
    15661 catctgcatg aggctgaggg cgccttcctg catccgcgct ccgcccgagc acgagatgat
    15721 gatgaaagga attttttcag ccatcgccct ctcggcggcc cgggtgatct tttccccgac
    15781 gaccgagccc atgcttcccc ccatgaaggc gaagtccagg gcggagatcg ccgtccgaat
    15841 gcccccgatc atccccagcc cgttgaccac ggcctcgggc agggaagtct tccgctggtt
    15901 gtccgccagg cgcttgtgat agggctggct gtcgacgaaa tgcaggggat cccggggcag
    15961 gatgtcgtgg tcggtgatat cgaaccgggc gtcgtcgtac agcaaggcca gccattccga
    16021 ggcggaaagc cggaaatgga acgcgcaggc cggacagacg gacaggttat ccaggatatc
    16081 ctccttgtaa aggatacgct ggcaattgtt gcatcgtgtc cacaagttgg tcatgacggt
    16141 tcaagagtct aacagcaagc ccgaacaggt gtcaacctcg ggccgggggg gcggccttga
    16201 gacgatcgga aagcaaacgg atgacctcgg ccgccgtcgg gaggtccact ccgaagtccg
    16261 acttttccct gagcaggtcg agagcggtct ggagaaggtg ctcgccgtcg aagggctttt
    16321 cgaagtacgc ggccgccccg aacgaggtta aggcctcctg cttgtactgc ggcccccggt
    16381 agagaccggt gacgatgatc accgatgtcc ggcccttgga ttcctgggtg atccgcttgg
    16441 tcaggtcgaa gccatggagc ttggggagga tggcttcaag gatcaccagg tcgggattct
    16501 cgattttgaa ggaatcataa gccgccgccc cgtcggaggc ctggaaaatc tgaaaacggt
    16561 acggctcgaa cagggcggcc acggcgtccc ggttcttcgt gtcgtaatcg accaggagga
    16621 ttttcttatc ggccatcgga aataaggccc tccgcgccgg gagaatctta ttatgaccga
    16681 acggcggcgg aaattcaatt cccggccgaa ttgtgctata gttgccccgt ttccatggcc
    16741 aaaggcatcg atctctccat catcgtcccc gttttcaacg aggaaaagaa ccttcgccct
    16801 cttcacgccg aaatcaaggc cgccggcgat aagctcggcc ggacctatga aatcatcctg
    16861 gtcgacgacg gcagccggga cggttcctgg gacgtccttc ggtccatcca ggcctccgac
    16921 gtccgggttc gggcgatccg gctccggaag aatttcggcc agacggccgc tctttcggcc
    16981 gggttcgaca atgcccgggg ccggatcatc gtcaccttgg acgccgactt ggaaaacgac
    17041 ccggccgaca tcgggatgct catcgacaag atcgaggagg gatacgacct ggtcagcggc
    17101 tggcgcaagg accgttggaa aaaacatttc ttcacccggc gcatcccctc gctcttggcc
    17161 aaccggttga tctcggccat caccaaggtc cgtctccggg atttcggctg caccttgaag
    17221 gccttccgcc gcgaagtcat cgaaaacatc cacctctacg gggagatgca ccggttcatc
    17281 gcggccatcg ccgcctccgt cggcgtcgcc atcgccgagg tcccggtgaa cttccgcccc
    17341 cgcatccacg gcaagtcgaa atacggcatc tcccgctcga tccgggtgat gctcgacctc
    17401 ctgacggtga aattcctcct cagctattcg acccggccgc tgcagatatt cggcctgatg
    17461 ggtctcatct ccggcgcggc cgggacggtc atcggactga ttctctccta ccagcggctc
    17521 gtcctgaaaa cagccatcgg caaccggccg cttctcctgc tggccgtcct gctcctggtc
    17581 gtcgggttcc agttcgtgac catgggcctc ctgggcgaaa tcatggtccg aacctaccac
    17641 gaggcggtcg aaaaacatat ctacgtcatc cgcgaggtca tcgaaacccc accgaccaag
    17701 gccgagtgag cccgtggcga tgaagatcct gatgctcgcc ccggagccgt tcttccagcc
    17761 ccggggaacg cccatcagcg tctatttccg tctgaaagcc ctgtccgatc tgggccattc
    17821 cgtcgatctg gtcacctacc cgcgcggcgg agacgtggcc tttcccggcc tggccatcaa
    17881 gcgcgtcccc aacctgatcg gcgtccgcga tatcaagatc ggcccctcgc tggtcaagct
    17941 tcccctggac gccctgatgt tcgcgacggc cttcgggtcg ctcctccgaa agcgctacga
    18001 tctcatcttc tcccacgagg aagccgccct cctcggcgtc ctgctcggga agatcttccg
    18061 cactccgcat ctctacgaca tgcactccag cctcccccag cagctcgaaa acttcgattt
    18121 ttcccggagc gccctcctga agacagcctt cctccggatg gagcgcttca tcctcaaggg
    18181 atcgcgctcc gtgatcgtca tctgccgaga cctgctcgac tatgccgccc ggctgggatt
    18241 cggcggcaag gccgtttttc tcgaaaacat cctggacttc aacgatttcg atccggcgcc
    18301 gcccggtccg gtggaaatcg ccgccgtccg gcgggatatc gccggcgacg gtgaaaaaat
    18361 cgttctctac gcgggcaatt tcgagcccta tcagggcgtg tctctgctcg ttgaagcctt
    18421 cggccggatg aaggagcgag ccgttctttt aatcgtcggc gggtcgaagg ccgaccacga
    18481 accggcccgg aagcgggcgg ccgagctggg cttcgccgac cggttcgtcc tcgtggaaaa
    18541 agtgccgccc cagcgcgtgc ccgtctatat cagtctgagc gacgtcctcg tctcaccccg
    18601 cgtctcgggg accaacacgc cgctgaaaat ttacgcctat ctcaaagcgg gcaagcccgt
    18661 cgtggccacc gacctgtgga caaacagcca ggttttgacg gaggagatcg ccgtcctggc
    18721 gccgcccgat cccgaaaaat tcgccgccgc cctggacttc gccctgacat ccgcggaggc
    18781 ggagcgccgg gcccgggccg gggcggacct ggccgcccgg gagtacacgt acccgcgcta
    18841 caaggaaaag atcaccgagg cggtcacgag ggccgtccgt cgatgaacgc cttcgtcacc
    18901 gggggaacgg gtttcgtcgg aaatcatctc tgtgcggccc tcgcggcctc cggctggacg
    18961 gttaaagcgc tcggccgcag gcttcggaac aaccctcccc cgggcgtgga gttcgtctcc
    19021 ggcgacatcc gcgacgccgc cctcctggat cgggcttcgg ccggggccga cgttgtcttc
    19081 cacttggcct ccgccctcgg ggccgcccgg atcggggccg cggaattcat ggccgtaaac
    19141 gccggcggga cgcgggcggt gttggcggcc gcccgggaaa acggcgtccg ccgggtggtt
    19201 cacttcagct cggccggagt cctgggccat gtccggcccg gcgaaaccgc cgacgaaaaa
    19261 tatcccctcg atccccggaa cgtctacgac aggaccaagc tcgccggcga gcgcgccgcc
    19321 ctggaagcgg cctcggccgg ccaggacgtg gtcgtcatcc gtccgggctg gatctacggg
    19381 cccggcgacc gccggacttt caagctcatc cgatcgatcg ccgggggcag gtttctgctg
    19441 cccggccggg gcgaaaccct ccaatctccg gcgtatatcg acgacctggt ggccggaacg
    19501 cttctctgcg ccggccgcgg acgggcgggg gaaatttata atatcgccgg aaaccaggcg
    19561 atgcccgtcc ggaagatggt cgagacgatc gccgccgccc tcggaaagag cctgccgcgc
    19621 ttccatcccc ccatgggact gacccgggcg gccggggcgg tgctggggat cctgttcggc
    19681 ttcgccggga aagaagctcc gctcaatccc tcgcgcctgg cttttttcgt ccaccccaag
    19741 ggcttgaaca tcgacaaggc ccggagggag ctcggcttcg aaccggccgt cgggttcgcc
    19801 gaggggatgg cccgggcggt ggattggtat cgccgggccg gctggctctg agggggtcct
    19861 ccccggttat catttaaagg gatgaatatc agccggatat ccgggccgga aaatatatag
    19921 gtgattcaac cggaataaaa acctggaatg cgcctcgagc gcgaatcccg cttcttcgca
    19981 catcctgatc accgctttct tcgaaagccc gcccgtttct cccgtcttca tgccgccccg
    20041 cttcttgtct tcgctgtacc accaaattcg atggccgatt cctcccaaaa cgggaccgat
    20101 catggtgagg atcattttcc cggacggccg gaccaagcgg cgcgcc
//
LOCUS       DAOOXH010000040        19877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_2601_length_19877_cov_7.731157, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000040 DAOOXH010000000
VERSION     DAOOXH010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 19877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19877
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2601_length_19877_cov_7.731157"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..372
                     /locus_tag="PKV64_06260"
     CDS             4..372
                     /locus_tag="PKV64_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HPN16719.1"
                     /translation="MLVLGGLVFSAALAQAPPEKKAPAPAKSPLPMEQLAEAMAQRLA
                     ESLQVKTAIGDPIKAGKVTLVPIIMIDIGFGGGGGGAPQNPEMEGKGFYMGGEARPLG
                     FIVVTKSGTRFVSVGKIPRK"
     gene            383..1321
                     /locus_tag="PKV64_06265"
     CDS             383..1321
                     /locus_tag="PKV64_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HPN16720.1"
                     /translation="MNAQRVLIVSFFTLFFGAGILMAQAPLEKPLADFDRLVSGLKVG
                     AVVGQPVTVQDTVIVPFAKIKFGLGGGGAMMSFGGGMSGKTVPLGVLIIEGENVRVEV
                     FPEEEPKPSLLQELLPILLKALPQMIGNKMPGTTTPPTSESAAGTAAAALPKDASLDQ
                     AKKLFEEKKYPQALDMIEALIAKEPGKADFHAWKGHIMGNMAQGNPLDMMKYGMGAMR
                     EYEAALQLDPKNADAHFGRGMARMSAPPGFGGDLDGAIEDFEAALIKPCPETYFHLGE
                     AYKKKGLNDKAKEAYRKALALDPEYAEAKKALEKLG"
     gene            complement(1465..2745)
                     /locus_tag="PKV64_06270"
     CDS             complement(1465..2745)
                     /locus_tag="PKV64_06270"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPN16721.1"
                     /translation="MKMKQMGAGLILLIMMTVAAAAGNVEETFAVCGGRVVPIAGPEI
                     ENGVILVENGKIKAVGAASEISIPPGIPIIDAAGGWILPGLVDSFASAGQAGGDEISD
                     PVTAGLRILDDLNPFDKRFEQTARAGITTLMVGPGRVNVIGARTAVIKPRGVTAEDMV
                     LLEPGGVKISLGEGPKAAFGEKGRLPGTRMGSAYVVRKALLEAAEYNHKRRQAETDKA
                     AAARKKDAKPASPLARNLDLEPLADLLEGKLPAFIECHRVDDIQTALRLVDEFGFRAV
                     LFGAEEGWKIADQIAGRNIPVVVGSMGVGPKREETKNVKLGNAAELVKAGIKVAIGAE
                     DSLGIGAREELVLSAALAVKAGLDRPTALRAVTLTAAEILGVASRVGSIEPGKDADLV
                     IFTGDPLHYQTRVARVLIEGRPIPVPEQLRKPRP"
     gene            complement(2754..4025)
                     /locus_tag="PKV64_06275"
     CDS             complement(2754..4025)
                     /locus_tag="PKV64_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPN16722.1"
                     /translation="MIHRKTISAAIAAALIMAAPMVQAMEAEEITAVVGARVLTAAGL
                     EIDNGVILIKEGKIAAVGAAGDISVPAGARIIAGAGLVVTPGLIDSHSHLGLGPSGGV
                     TEDNETSDPITPQLRIIDSIHPEGVGPDQHQFRNALAEGVTTVAARPGSSNVIGGQSA
                     VLKLRGGTVDDMILRFPSDMKMALGRKGYYASKGVMPTTKMGAVFLARKALVEAEEYG
                     KAQDRRKKAGTADSKDVPARDLKKEALLKVIRREIPVHIHVNSADDIMTAARLSDEFG
                     FLRLSLGHAVDAYKVAGELARRGIIVVVGPQMITYDDQGRLVNLAGYLKDKGVQVDIM
                     TDADVVQQAFLRCQAGVAVKYGMLPQDALRSITINPARTLGLEDRIGSIEPGKDADLA
                     FFDGDPFDPASQVVMVFIDGRAVYEAEKEPK"
     gene            complement(4278..5201)
                     /locus_tag="PKV64_06280"
     CDS             complement(4278..5201)
                     /locus_tag="PKV64_06280"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HPN16723.1"
                     /translation="MKRLVNILEAIGRTPLVDISGLVEAKHGIRLLAKIEGLNPGGSI
                     KDRTSLFMIREAEDQGLLRPERTILEPTSGNTGIALAMIASVLGYQAHLVMPACVSSE
                     RRAIIAAFGAEVDLTPAEAGTDGAIARARELAAADPGKYFMPDQFANPANVRAHYETT
                     GPEIFRQAEGEVDVFVAGLGTGGTLMGAGEYLKAAKPGIKIFGVEPLPGHRIQGLKNM
                     SESEKPSIFNRSLLDGIIAVDDEEAFVAARELVTRAGVFAGLSSGAAVAGARKIAADF
                     PGAVIATVLPDRGERYLSTEIFRSYCGRCRP"
     gene            5525..6253
                     /locus_tag="PKV64_06285"
     CDS             5525..6253
                     /locus_tag="PKV64_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16724.1"
                     /translation="MAAASECPICSRLRDVETSFSKYLAPQLDNPLPEAAGRLVVLPD
                     PSGQDPEKRHTRRCPACGTLYDYVSCYEYHMNGGEESEELTRLNPDQSASFLFSQARA
                     LEERRREINLLEDSAGGLGDLIDRGRPEAGEVREAAAEMETLRRRADSLRLRLRELVE
                     ALRRACPEILFTWAGAHGRVCRKFLDAPGDGGEDSRMARFEARSSLDFWEKLPFGGET
                     YIAGNSPWLPGYDDLLDRELAPER"
     gene            complement(6295..7455)
                     /locus_tag="PKV64_06290"
     CDS             complement(6295..7455)
                     /locus_tag="PKV64_06290"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HPN16725.1"
                     /translation="MSITRRDFVRLAGMGATAAAAGLATASANTGQPEPQAASGGLSP
                     EERGIHPILEKDHPYIFIDSCMQIWPDAPFADAHRYGATVYGVTAWDPHLDVDQALEG
                     LMFWRAAARKHPNLAVVTTAAEIPDLKKAGRSGLLLASQCGDFMGDKLHRLEAFYRLG
                     LRMLIFAYSQSNRLCGGCLDQADGGLTSLGELAVAECNRLGLLLDCSHVGARSALEII
                     ERSAQPVVFSHSGAEAVSSNPRNIDDDRIKACAAKGGVVGLCPWGPMLLKKGRTTRPT
                     VDDFLNHIDHAAQLVGGVDHIGVGTDMSLGTYPEHKVDPWGTPALKDVTGSYDKHISA
                     NMRSPLRMAEGFSQYADVVHFAERLGKRGYGDAGIRKILGGNFLRVFSQVWK"
     gene            7949..8599
                     /locus_tag="PKV64_06295"
     CDS             7949..8599
                     /locus_tag="PKV64_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HPN16726.1"
                     /translation="MKRPFINGLGLLGVVSLLSYAAAVVWAPSAYPGYDWMRQAVSDL
                     SAANAPSKDLWNQLSCLYGVGGLVSIMMACVFIQGKLNKTLRIGIYAFAAMNWVSAVG
                     YPLFPLSASGYAGSFQDMMHVYVVTTLVVILSLVSLILIMVGGYRRNGYRSLAIWASI
                     ALAAMGAGAVGTSVAPRGYFALFERLSLFAATGFNAVLGVYLFNGFRSHRSELEKA"
     gene            8660..9046
                     /locus_tag="PKV64_06300"
     CDS             8660..9046
                     /locus_tag="PKV64_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16727.1"
                     /translation="MASLGSPAKSEETASLSIYPPAGPVNQQIRRTMIVSFHGVAPHS
                     SILRIVNRPQSPFFKGEISDNACRKADNDRFNEKPRSFLVESDDFDLCPRNRLDGRLL
                     GHRSSAEDHSFALIVLSFSGDHPFPC"
     gene            9252..9623
                     /locus_tag="PKV64_06305"
     CDS             9252..9623
                     /locus_tag="PKV64_06305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPN16728.1"
                     /translation="MPAYFDWTNFELLRQYPKNMVLWTCIGYGVFNVFVGPVVEELFF
                     RGYLTSKISRFGRRAPIIMTVLFSLYHLWLPFQNLFRISAFLPAAYMAWKENNISIAI
                     VFHCLCNLVSTVSFILVLYSV"
     gene            complement(9775..10320)
                     /locus_tag="PKV64_06310"
     CDS             complement(9775..10320)
                     /locus_tag="PKV64_06310"
                     /inference="COORDINATES: protein motif:HMM:NF039476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6512 family protein"
                     /protein_id="HPN16729.1"
                     /translation="MHQTTDRRAIAVWEAAGAVFIILAGSALHFVFVWSGKQRPIAWL
                     AAVNESTWEHFKLAFWPALLFALVELPFLKKRTGNFWTAKAAGLWAMPVVIGAVFYAY
                     RAVLGRHFLWADILLFVVAVCAGQAASVRILTAPPQPRTRRRLAYFALVLLFLAFALF
                     TYFPPKIFLFADPRNGGYGIL"
     gene            complement(10334..13159)
                     /locus_tag="PKV64_06315"
     CDS             complement(10334..13159)
                     /locus_tag="PKV64_06315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPN16730.1"
                     /translation="MRGYLLRNRVPAAAVAALLALALPARAQVLPFEVLGLKEGIPQS
                     QVSALAQDREGYLWVGTWGGLARFNGAEFKSFFREDGLPSTRIQELLVASDGTLWIAT
                     VAGVSRIAGRRLEILPDQAVSMVRCRALAEDARKRIWVGTDDGPAVFDGETWFLFHPG
                     SREKGPVVYDILSDREGVLVAAANGLWRFSGDAPPDAVVSPAGLEPADFRALAVTAEG
                     LWLGTAGAGVWLRDGADWRKITGSPVPRAVYSLTVQPSGTLYAATVDDGVFLKRPGAS
                     VFEHWGVENGLPSNLVSVVLEDREKSVWVGTDIGGLARLSNLAVINHTGNQGLPSACV
                     FGITPGEDSDSLWLGTMRGAVHYLVRPHPRVLETIRAADGLDNEWVWKALRTADGTLW
                     ILTDTALHFRRPGEKAVHPLPPHVSFPRTNPYDIVLDGAGNLWGCGEWSGGGLARRDA
                     GGTWRIWNTTVTGEPLTAVHHLARSRRGGVWAVAKSRFFYCDGETVAPLEIRCPLETG
                     VNISSILEDSAGRLWAGNDAGLAVLEAGGRWRLFNAKDHPGFANHHVFSIGEDWKGTI
                     WVNTARGVFRFLSEYRVEAFTPDDGLADWETNAQGFFNDSRGDIWIGTVNGLSQYNPT
                     GRIPNTEPPGLAIESVRLPGRTVEYPAGLDLAWNERTIVCNVAVLSFRGRSRAAYRAR
                     LEGLEGDWLPLSRREELRYTNLPAGDLKLLLQPVNESGVWGEVVTLPIRVRPPFWMTG
                     AFRAGAVVFLLAAAFAAYRWRTLLLLRRNRELQREVGKRTAELEYLAAYDPLTALYNR
                     RAVLAFLERELKPERGGNRRLGCIMVDLNRFKQVNDTLGHAAGDQVLKDMAGKIKECL
                     RPGDALGRLGGDEFLVVLPGADGAALKSVEGRISGLACQAGEGSAAVTVTAACGSVEV
                     PPATNAAVAAVLAAADDLLFQVKRSRR"
     gene            13691..14455
                     /locus_tag="PKV64_06320"
     CDS             13691..14455
                     /locus_tag="PKV64_06320"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPN16731.1"
                     /translation="MSKKLFPFAVFIAVFLGAAFAPGQSEDPKARELARKIDDLYRSD
                     SSYARIEMEIVTPDWRRTLALKGWTQGQSKTLIRILEPKKEAGIGTLRLGNEMWNYLP
                     KTDKVIKIPPSMMMSSWMGSDFTNDDLVKEFTFVDDFTFRMVTPPEAEKGILYLECRP
                     KPGVAVVWDKIVIAVAEADTLPVWQKFYDEKGQVARIMSYSEVRTFGRRTVPAVMEMV
                     PRTKTGCKTVLRYREIQFDLSLKDDLFSLRALQSGK"
     gene            14466..15680
                     /locus_tag="PKV64_06325"
     CDS             14466..15680
                     /locus_tag="PKV64_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPN16732.1"
                     /translation="MIKMAFRNIFRQKRRSLLTTLTMFGGFVLASLALGVIDGTYNRI
                     VDEFTRSRMGHIQIHARGYLERPTLQKAIRNGREIGVEIDRSRDAEAWAPRIIAAALA
                     SVGERTDTARILGIDPVRENAATRFERKITRGRSFSPDPSKEIILGPGLAQALKAAIG
                     DEVVLVSQAADGSLANDVYRLIGLADTGDMATDRTSAYLHLEDARSLFVLEEAVHEIV
                     VVSRSLKKATNLNASLRESLAAFPVDVQPWQEFAKSFYNAMQADSRGHEMMLLILFIV
                     VSFGVLNTTLMSVLERRREYGVLKALGTRPRQVFRLILCEVLLLAAASVVLGAGAGFL
                     INSAVAQHEIVIFSKGVTFGGMEMRSFTTEVNARTLAIPALTVLFSALFVSLWPAVRA
                     ARTEPARSIRIF"
     gene            15691..16917
                     /locus_tag="PKV64_06330"
     CDS             15691..16917
                     /locus_tag="PKV64_06330"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPN16733.1"
                     /translation="MSLYSRLAWRNIFRNKRRTYITGIAVAVGLAALMFLDALYIGMA
                     DNMIAAATASFTGEAQIHGRDFLLARDANLTVAGSERLLADLRNDTLVKAFTPRTLSQ
                     GMVTSAANVNPVLLVGVRPDTERALSQMDDVIEAGDYFGGNDPQNIVIGRKLADLLEV
                     GVGDRVVVTVSRAGSGELSQDLFRVSGIYRFNTDDLDDGLAFVRIEKAQDMLGLAGRV
                     NEIALSFKDPRVSQDETHPFWTDYSTGGNEALGWPRLFPALKSVYQMTTAASLILGLL
                     LFTVVALGIVNTMFMSIYERMFEFGVLRAVGTRPGGIRRLVILEAGSLALLSIALGVG
                     LGFLVTLIVSLTGVNYQNIEFAGVTIKELIYPVFHLKQYVIFPAGVLLFTLLVGLYPA
                     GFAARMSVTRAMKRTM"
     gene            16930..17631
                     /locus_tag="PKV64_06335"
     CDS             16930..17631
                     /locus_tag="PKV64_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN16734.1"
                     /translation="MNPQHRHLIETEGLIKTYSDNGVQVPALRGVDLTIHAGEFTAIV
                     GPSGSGKTTLLNIISGLDKPTSGKVWLDGRLVSDMSGAELSDFRRDHIGFIFQAYNLI
                     PVLKVEENIEYIMLLQGVPKEERHRRVHDILRDVDLEDYAHRYPNRLSGGQQQRVAIA
                     RAMVSHPLMILADEPTANLDSKTGADLLDMMRHLNAEHGMTFIFSTHDRMIVDKAKRV
                     IVIRDGIVASDETRN"
     gene            17639..18910
                     /locus_tag="PKV64_06340"
     CDS             17639..18910
                     /locus_tag="PKV64_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16735.1"
                     /translation="MRRPRASIGAGALAAALAMAALPPAAEGELKLGGYLKNHSVVFR
                     LPESKTAASDLPLPGISQFRLRVFLAGDLSPDVSFQAAYDLSPRIQDTLFFRASPFIP
                     SPEAGDYRAADFRSRLVPAPGKPAGSFGLYHNLDRLSLSVKMTFGDLILGRQPVAWGS
                     GRITNPTDLIAPFSFQELDKEERFGVDAVRLRIPLGALSELDAGAVFGRDLEFKRSAF
                     FLRGKVNVLETDAALLLLGFREDLLLGLDLARSLGGAGVWLEGACVFPDALNGKKPKA
                     SSYARLTAGIDRSLSDRAYAFFEYHYNSAGSSEPGDYTALFSRPAYTRGAAYLMGRHY
                     LAAGLTWQATPLIPVSGLLLWNATDGSLALSPQAEYNIAENIYLGAGAFVGVGRPPLR
                     SPFDATGTGPVLDSEFGSYPDFVFLSFRVYF"
     gene            18924..19589
                     /locus_tag="PKV64_06345"
     CDS             18924..19589
                     /locus_tag="PKV64_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPN16736.1"
                     /translation="MKTRARTKTGTTAAKKARNPYPPGGREDAIRQFILEHSEHLFVT
                     HGYGKTSMDMVARECGLSKPTLYKYFKNKYEMFTSLYERLYRSLNEMFKLILGRNRDK
                     AQVLEEIILGYFLLMTSKKDFLKMYFREQHLVVHENIEEHMSWHVESRKKMEEMLAQC
                     LQGILRPDIAKRYGSAMVASTLFDILEGMVSNLILHGEQDPAKEKAFILEFLRSGVLA
                     RSA"
BASE COUNT         3726 a         6255 c         6116 g         3780 t
ORIGIN      
        1 gctctgttgg ttctcggcgg gctcgtcttt tcggccgcgc tcgcccaggc acccccggaa
       61 aaaaaggccc ccgctccggc caagtctccc ctgccgatgg aacagctggc cgaagccatg
      121 gcccagcggt tggcggagag tcttcaggtt aaaaccgcga tcggcgatcc gatcaaagcc
      181 gggaaagtca ccctcgtccc gatcatcatg atcgatatcg gcttcggggg cggaggcggc
      241 ggcgcgcccc agaatccgga aatggaaggc aagggcttct atatgggcgg agaggcccgg
      301 ccgttgggat tcatcgtcgt caccaagtcc ggaacgcgat tcgtaagcgt cggcaagata
      361 ccgcgcaagt aaggaggatg ccatgaacgc ccaacgagtt ctcatcgtct cttttttcac
      421 gctctttttc ggcgcgggca tcctgatggc tcaggctccg ctcgagaaac ccctcgcgga
      481 tttcgaccgc cttgtctccg gcctcaaggt cggcgccgtt gtcggccagc cggtcacggt
      541 ccaggacacg gtcatcgtcc ccttcgccaa gatcaagttc ggtctcggcg gcggaggagc
      601 tatgatgtcg ttcggcggcg ggatgagcgg caagaccgtc cctctgggcg tgttgatcat
      661 cgagggcgag aacgtccgag tcgaggtttt cccggaagaa gaaccgaagc cgtccttgct
      721 gcaggagctt ctcccgattc tgctcaaggc cctgccccaa atgatcggga acaagatgcc
      781 ggggacgaca acgcccccga cgtccgaatc cgcggcggga acggcggcgg ccgctctccc
      841 gaaggacgct tccctggatc aggccaaaaa actatttgag gagaaaaaat atccccaagc
      901 cctggacatg atcgaggccc tgatcgccaa agagccgggc aaggccgatt tccacgcctg
      961 gaagggacat atcatgggga atatggccca gggcaatccg ctcgacatga tgaaatacgg
     1021 aatgggcgcg atgcgtgaat atgaagccgc gctccagctg gacccgaaaa acgcggacgc
     1081 tcatttcggc cggggcatgg ccaggatgtc cgcccccccc ggattcggcg gggacctgga
     1141 cggagcgatc gaggacttcg aagcggccct catcaagcct tgccccgaga cttattttca
     1201 tctcggcgaa gcctataaga aaaagggcct gaacgacaag gcgaaagagg cttaccgtaa
     1261 agccctcgct ctcgatcccg aatacgcgga ggcgaagaag gccctcgaga agctcggctg
     1321 atatcaacgc caggagatga agatccctcg gccgccggcc cgaagccgcg gggggcggac
     1381 gtcatccccc aaacgagggg aagccgggcc gcctcctccg cggccggagg ataggccgcc
     1441 tcgggctgga cgaaaccggc ggttctacgg cctcggcttc cgcagttgct caggaaccgg
     1501 gatcggccgc ccttcgatca agacgcgggc gacgcgcgtc tggtaatgca gcggatcgcc
     1561 cgtgaagatc accaggtcgg cgtctttccc cggctcgatg cttccgacgc gggaggccac
     1621 gcccagaatc tcagccgctg tcaaagtcac cgcccggagg gccgtcggcc ggtcgagacc
     1681 ggctttgacg gccaaggccg ccgacaggac aagctcctcc ctcgcgccga tcccgagcga
     1741 gtcctcggcc ccgatggcca ctttaatccc ggccttgacc agctcggcgg cgttgccgag
     1801 cttgacattt ttcgtctctt cgcgtttcgg gcccacgccc atcgatccga caacgaccgg
     1861 aatattccgg ccggcgatct gatccgcgat tttccatcct tcctcggccc cgaacagcac
     1921 ggcccgaaag ccgaactcgt cgaccaggcg aagagcggtt tgaatatcat cgacccggtg
     1981 gcattcgatg aaggccggaa gcttgccctc tagaagatcg gccagcggtt cgagatcgag
     2041 attccgggcg aggggcgacg ccggtttcgc gtcctttttc cgggccgcgg cggctttgtc
     2101 cgtctccgcc tgccggcgtt tgtggttata ctcagcagcc tccaacaggg ctttgcggac
     2161 aacgtaggcg ctgcccatgc gagtcccggg caggcggccc ttttccccga acgcggcctt
     2221 cggtccctcg cccaacgaga ttttgacgcc gccgggttca agaagaacca tgtcctcggc
     2281 cgtgacgcct cggggcttga tgaccgccgt gcgggcgccg atcacattga cccggcccgg
     2341 cccgaccatg agcgtcgtga tcccggcccg ggccgtttgt tcgaaccgct tgtcgaaggg
     2401 attcaagtca tcgaggatcc gcaggccggc cgtgacggga tcggagatct cgtcgccgcc
     2461 cgcctgcccg gccgaggcga aggaatccac cagccccggc aggatccagc cgccggccgc
     2521 gtcgatgatc gggattcccg gcgggatgga aatttcggac gccgctccga cggccttgat
     2581 tttcccgttt tcgacgagga tgacgccgtt ttcgatttcg ggtccggcga tcggaacgac
     2641 gcggccgccg caaacggcga atgtctcctc gacgtttccg gccgcggccg cgaccgtcat
     2701 catgatcaac aggatcaaac ccgcgcccat ttgtttcatt ttcatgttcc tcttcacttc
     2761 ggttcctttt ccgcttcgta aaccgcccgg ccgtcgataa ataccatgac cacttgggaa
     2821 gccgggtcga agggatcgcc gtcgaagaag gccagatcgg cgtctttgcc gggctcgatg
     2881 cttccgatgc ggtcttcaag ccccaaggtg cgggcgggat tgatggtgat ggagcgaagc
     2941 gcgtcttgag ggagcatgcc gtatttcacg gccaccccgg cttgacagcg aaggaacgcc
     3001 tgctggacga cgtcggcatc ggtcatgatg tcgacttgaa cccctttgtc cttgagatag
     3061 cccgccaagt tgaccagccg gccttggtcg tcgtacgtga tcatctgggg gccgacgacg
     3121 acgatgatcc cgcggcgggc cagttctccg gcgaccttgt aggcatcgac cgcgtggccc
     3181 agcgagagcc tgaggaaccc gaactcgtcg gacaagcggg cggccgtcat gatgtcatcg
     3241 gccgagttga cgtggatatg gaccggaatc tcccgccgga tcaccttgag cagggcttct
     3301 ttcttaagat cgcgggccgg gacgtctttc gagtcggccg ttccggcctt cttccgccga
     3361 tcctgcgctt tcccgtactc ttccgcctcg acgagagcct tgcgggccag gaaaacggcc
     3421 cccattttcg tcgtcggcat gacgcccttg gaggcatagt atcccttccg gccgagggcc
     3481 attttcatgt cggaggggaa acgaaggatc atatcgtcga ccgttcctcc cctgagcttg
     3541 agaaccgctg attggccgcc gatgacgtta ctgctgccgg gcctggccgc gacggtggtg
     3601 acgccctcgg ccagggcgtt gcggaactgg tgctgatcgg ggcccacgcc ctcggggtga
     3661 atggaatcga tgatccgcag ctgaggcgta atcggatcgg atgtttcatt gtcctcggtg
     3721 acgccgcccg agggtcccag ccccaagtgg gagtgggagt cgatcagccc cggcgtgacg
     3781 acgaggccgg cgccggcgat gatccgggcc ccggcgggga cggagatgtc gccggccgct
     3841 ccgaccgccg cgattttccc ttccttgatc aggatgacgc cgttgtcgat ttcaaggccc
     3901 gccgccgtca gaacgcgcgc tccgacgacg gccgtgatct cttccgcttc catggcctgg
     3961 accatgggcg cggccataat cagggcggcc gcgatcgcgg ccgagatcgt tttcctgtgg
     4021 atcatgggaa tccactcctt atcacgattt gcggacatga gccgaaacgt gccggccgcc
     4081 gctccgccca tgatatcacg cccgcggcct cgtggggaga tccgcattct ccttcggctc
     4141 tggaatccgg ccttgcgccg catttaaaaa caacgatgtc ccccgcttat tgctcgagcg
     4201 cccggccctg ccggtcgata atcattactc gaatagaacg ccgaacatgg ggcttgaatc
     4261 cggaattccc ttagttttca cggtcggcag cggccgcaat aagaccggaa gatttccgtg
     4321 ctgagatacc gctcgcccct gtcgggcaga acggtcgcga tgaccgcgcc gggaaaatcc
     4381 gcggcgattt tccgggcgcc ggccaccgcc gccccgctgg acaggccggc gaagacgccg
     4441 gcccgggtca cgagctcccg ggccgcgacg aacgcctcct catcgtcgac ggcgatgatc
     4501 ccgtccagaa gggaccggtt gaagatggac ggcttttcgg attcgctcat gtttttcaag
     4561 ccctgaatgc gatggccggg aagcggctcg actccgaaaa tcttgatccc gggcttggcc
     4621 gccttcaggt attcccccgc gcccatcaag gtgccgcccg tgccgaggcc ggccacgaaa
     4681 acgtccacct ccccttccgc ctggcggaaa atctccggtc cggtcgtttc gtaatgagcc
     4741 cggacattgg ccggattggc gaactggtcg ggcatgaaat atttcccggg atcggcggcc
     4801 gccagctccc gggcccgggc gatggccccg tccgttccgg cttcggccgg agtcaggtcc
     4861 acctccgcgc cgaaggcggc gattatcgcc cgccgctcgc tggaaacgca agccggcatc
     4921 acgagatggg cttgatagcc gagaacggaa gcgatcatgg ccagggcgat gccggtattc
     4981 cccgacgtcg gctcgagaat cgtccgctcc ggccggagaa gcccttgatc ctccgcttcc
     5041 cggatcatga acagggaagt ccggtccttg atggatccgc ccggattgag gccttcgatc
     5101 ttggccagaa ggcggatccc gtgtttcgcc tcaaccagcc cgctgatatc caccagcggc
     5161 gttcggccga tggcttctaa aatattcaca aggcgtttca tattcatcat gctcctgtcg
     5221 attgacggac aacatcatgg aaagaaaaac ggcggcgggg gcgggggatt cagcgggcgg
     5281 cgggtcgccc gcagcagaga cagccgagaa cggagagccg ggataggatt ccgcggtttc
     5341 tttggctgtc gatcgtcatg acgggcggca ttttagcgca ttcccgggcc gccggcaaga
     5401 aaaatcttgg ttaaacgaga ggatcggcat cataggcgaa tcgattcagg gccggcggcg
     5461 gtcggatcga ggccgcttcc ttgtgtcctt gccgtcccgc cgggagcccc gtataatcga
     5521 ggccatggcc gccgcaagcg aatgcccgat ctgcagccgg ctccgggatg tcgaaacctc
     5581 cttttccaag tatctcgccc cgcagctcga caatccgctt cccgaagcgg ccggacggct
     5641 ggtcgttctt cccgatccct cgggccaaga cccggagaaa cgccataccc ggcgctgccc
     5701 cgcctgcggc acgctctacg attacgtcag ctgttatgaa tatcacatga acggcggcga
     5761 agagagcgag gagctgacgc gcctgaatcc ggaccaatcg gcctcgttcc tcttctccca
     5821 agcccgcgcc ctggaagaac ggcgccgcga gatcaatttg ctggaggact cggccggcgg
     5881 cctgggcgac ctcatcgacc gcggccggcc cgaagccgga gaagtccggg aggccgcggc
     5941 cgaaatggaa acgcttcggc ggcgggccga ttcgctgcgt ctccggctgc gggagctggt
     6001 cgaggcgctt cgccgcgctt gtccggagat attatttact tgggccggag ctcacggccg
     6061 cgtctgccga aaatttctgg acgctccggg cgacggcggc gaagacagcc gcatggcccg
     6121 cttcgaagcc cgctcctccc tggatttttg ggaaaagctg cctttcgggg gagaaacata
     6181 tatcgccggc aactccccct ggcttccggg ttacgacgat ttattggacc gcgagctggc
     6241 tccggaaaga tagggagaag agcatgggac gagggacccg gttctcctcc cggctcactt
     6301 ccatacttgg gaaaagaccc ggaggaaatt tccgcccagg atcttgcgga tgccggcgtc
     6361 gccgtagccg cgctttccca ggcgttcggc gaaatggacg acatcggcat attggctgaa
     6421 accctccgcc atccgaagcg gggagcgcat attcgcgctg atatgcttgt cataagatcc
     6481 ggtcacgtcc ttgagggccg gcgtgcccca cgggtcgacc ttatgctccg gatacgttcc
     6541 caggctcatg tccgttccga cgccgatatg gtcgacgccg ccgacaagct gggcggcatg
     6601 gtcgatgtgg ttgaggaaat catcgacggt cggccgcgtg gtccggccct tcttgagaag
     6661 catcgggccc caggggcaca accccacgac tccgcccttg gccgcgcagg ccttgatccg
     6721 gtcatcgtcg atgttgcgcg gattggacga gacggcttcg gctccggagt ggctgaagac
     6781 gaccggttga gcgcttcgct cgatgatctc cagggcggag cgggccccga catgggaaca
     6841 atccagaagc aatccgaggc ggttgcattc ggctacggcc agctcgccca gcgaggtcaa
     6901 tcccccgtcg gcctggtcga ggcagccgcc gcagaggcga ttgctctggc tgtaagcgaa
     6961 aatcagcatc cgcaggccca agcggtaaaa ggcctcgagc ctatggagct tgtcgcccat
     7021 aaaatccccg cattgggaag ccaagagcag cccggaccgg ccggcttttt ttagatcagg
     7081 gatctcggcg gctgtggtga cgacggccag gttcggatgc ttgcgggccg cggcgcgcca
     7141 aaacatcagc ccttccaggg cctggtccac gtccaggtgc ggatcccagg cggtgacgcc
     7201 gtaaaccgtg gctccgtagc gatgggcgtc ggcgaagggc gcgtcgggcc atatctgcat
     7261 gcaggagtcg atgaaaatat acgggtgatc cttttccagg atcgggtgga tgccccgctc
     7321 ctcggggctc aatcccccgg aagcggcctg cggctcgggc tgtcccgtat tcgcggacgc
     7381 cgtcgcaagg ccggcagccg ccgccgtcgc ccccatcccc gccaagcgaa cgaaatccct
     7441 ccgagtgatg ctcatgccgc gctatccttt cttcaaaacg attgcctcaa cgagttttat
     7501 acgcgaggcc ggcggggatg tcaaaaccgg agcggatcat tcaaggggtt gaatgaccgg
     7561 ccgaccatcc catcgcccgt tttctctaat ccaccatgaa gaacagattc aaatccgatc
     7621 cgcctcggat catggagggc ctggagagag gaatcgaaac ttcagcgcct ccgggccggc
     7681 ggtccgtctc aacccggcca aagcggcgga cggcgcctgc agaaagacgc tcatgggaaa
     7741 acaggttgtt tccagggggg ggcgattcta tgataaagtc atcccggtgt ttccacgccc
     7801 ggaaaacgct cgtgataacc gcgggggctt ggagacgcga agggcaatga aaagaatcca
     7861 aggcggaaat ctcgaggagc ctgccgtgtc ccgcggagga gaagccgcat cgagggatag
     7921 aaaagcataa aaacggcata aacaaaaaat gaaaaggccg ttcatcaacg gactgggttt
     7981 attgggcgtc gtcagcctgc tttcctacgc cgccgcggtt gtctgggctc catcggccta
     8041 tcccgggtat gactggatga ggcaggcggt cagcgatttg agcgcggcca acgccccctc
     8101 aaaagatctg tggaatcaat tgagctgcct ttacggcgtg ggcggactgg tttccatcat
     8161 gatggcgtgt gttttcatcc agggaaaatt gaataaaacg ctgcgaatcg gaatttatgc
     8221 ttttgccgcg atgaattggg tttccgcggt cggctatccc ctgtttccgc tttccgccag
     8281 cggatatgcg ggatcattcc aggatatgat gcacgtgtac gtggtcacga cgctggttgt
     8341 gatcctttcc ctcgtttcgc tgattttgat catggtcgga gggtatcgga ggaatggtta
     8401 tcgatccctg gcgatttggg cgtccatcgc tcttgccgcg atgggcgcgg gcgcggtggg
     8461 aacgagtgtg gccccgcgcg ggtatttcgc cctctttgag cgtctcagcc tgttcgccgc
     8521 gaccggattc aacgctgttc tgggagtcta tttattcaac ggatttcgtt ctcatcgttc
     8581 cgagctcgag aaagcctgaa cgggtatctg aaaggccggc ttggtgcgcg cggaagcaag
     8641 tcaaagagaa gaccgggata tggcttccct cggatctccg gcgaaatccg aggagaccgc
     8701 gtctctttcg atctatccgc ccgccggtcc cgtgaatcaa cagatccgcc gaacgatgat
     8761 cgtctctttc catggggttg ctccgcattc ttcgatcttg cggatcgtca atcgcccgca
     8821 aagcccgttc tttaaaggag aaatctcgga caatgcatgt cgaaaagccg ataacgatcg
     8881 attcaatgaa aagccgcgat ctttccttgt cgaaagtgat gattttgatc tttgtccccg
     8941 caaccgtctt gacggccgcc tattgggcca tcggtcatct gcagaagatc attccttcgc
     9001 tcttattgtt ctttctttta gcggcgatca tccttttccc tgctgaactg gcgatcatcc
     9061 tcttcgccgg gaaaaaggag ttcggccgtt attcattgaa gagcgccctt gtgaatcaga
     9121 aaaagccgaa agcggcgcgg acccttctga tcggagtcat gctctttggc ttggcgggaa
     9181 ttctgtcgat tgtcgtgaag cccttggaaa gccttctgac gtcgccgata tccggaaaat
     9241 taggggccgt cttgcccgcg tattttgact ggacgaattt cgagctcttg agacagtatc
     9301 ccaagaatat ggtgttgtgg acctgcattg ggtacggcgt tttcaacgtg tttgtcggcc
     9361 cggttgtgga agagcttttt tttagaggat acctcacttc aaaaatcagc cgatttggaa
     9421 gacgcgcgcc gatcatcatg accgtcctgt tttccctgta tcatctgtgg ctgccttttc
     9481 aaaacctttt ccggatcagc gcgtttttac cggccgcgta catggcctgg aaagaaaaca
     9541 acatctccat cgccatcgta tttcattgtt tatgcaactt ggtttccacg gtcagcttta
     9601 tcctcgtttt atattccgtc tagagaggaa gacgagattc atcgggggaa aggacacggc
     9661 ctcgttgaat acgaggggtt ttctcccgcg ggcggatcat tgcgccggcc gccgggaaac
     9721 ggcctgcctt gccgggcgat tttgaaatcc gccgggtcga attccgcaca agcgtcagag
     9781 gattccgtat cccccgtttc gcggatcggc gaaaaggaag atcttcgggg gaaaataagt
     9841 gaacagggcg aaggcgagga aaagaagaac caaggcgaaa taagccagcc gccgccgcgt
     9901 ccggggctgg ggaggagcgg tcaggatccg aacgctcgcc gcctgtccgg cgcagacggc
     9961 gacgacgaac aagaggatgt cggcccaaag gaaatgcctc cccaggaccg cccggtacgc
    10021 gtagaagacg gcgccgatga cgaccggcat ggcccagagg ccggcggcct tcgcggtcca
    10081 gaaattcccg gtccgctttt tcagaaacgg aagctcgacc agggcgaaga gcagggccgg
    10141 ccagaaggcc agcttgaaat gctcccacgt gctctcgttg accgcggcca gccaagcgat
    10201 cggacgttgc ttcccgctcc agacgaacac gaaatggagg gccgagccgg ccaagatgat
    10261 gaagaccgcc ccggccgctt cccagacggc gatcgccctt cggtctgtcg tctgatgcat
    10321 ccgcaaacct ccctcagcgg cgcgaccgct tgacctggaa aagcaagtcg tcggccgcgg
    10381 ccaggaccgc ggccacggcg gcgttcgtcg ccggaggaac ctccaccgag ccgcaggcgg
    10441 cggtgacggt cacggccgcg cttccctcgc ccgcctgaca ggccaagccg ctgatgcggc
    10501 cctcaaccga tttcagggcc gccccgtcgg ccccgggcag aacgaccagg aactcgtccc
    10561 cgcccaagcg gcccagggcg tcgccgggac gcaggcactc cttgattttc ccggccatgt
    10621 ccttgaggac ttgatctccg gcggcatgac ccagggtgtc gttgacctgc ttgaagcggt
    10681 tgaggtccac catgatgcag cccaggcggc ggttcccgcc gcgctccggc ttcaattccc
    10741 gctcgaggaa ggccaggacg gcccggcggt tgtaaagggc ggtcagcggg tcatacgcgg
    10801 ccaggtattc cagctcggcc gtccgcttgc cgacctcacg ttggagctcg cggttgcggc
    10861 gcagaagcag gagggtccgc cagcggtagg cggcgaaggc cgcggccagg aggaagacaa
    10921 ccgcgcccgc gcggaacgcc ccggtcatcc agaacggcgg ccggacgcgg atgggcaggg
    10981 taacgacctc gccccagacg cccgactcgt tgaccggctg gagaaggagc ttcaaatcgc
    11041 cggccgggag gttggtgtaa cggagctcct cccgccggct caagggcagc cagtcgccct
    11101 ccaatccctc caggcgggcg cggtaggcgg cccggctgcg gcctcggaag gagaggacgg
    11161 cgacgttgca gacgatcgtc cgctcgttcc aggccaggtc gagcccggcc gggtattcca
    11221 ccgtccggcc gggcaggcgg acgctttcga tcgcaagccc gggcggttct gtattgggga
    11281 tgcggccggt cggattgtac tgggacaaac cgttgacggt tccgatccag atgtctccgc
    11341 gtgaatcgtt gaaaaagccc tgggcgttgg tttcccagtc ggccaggcca tcgtcgggcg
    11401 tgaaggcctc cacccggtac tcggacagga agcggaaaac gccgcgggcc gtgttgaccc
    11461 aaatcgtccc cttccagtcc tcgccgatcg agaacacgtg atgattggca aagccgggat
    11521 gatccttcgc gttgaacagg cgccaccggc cgccggcctc gagcacggcc aggccggcgt
    11581 cgttgccggc ccagaggcgg ccggcgctgt cctcgaggat ggaggagatg ttgaccccgg
    11641 tctccagggg acaacggatt tcgagcgggg cgacggtttc gccgtcgcaa taaaagaacc
    11701 ggctcttggc cacggcccag acgccgcccc gccggctccg ggccaagtga tggacggctg
    11761 tcagaggttc ccccgtcacg gtcgtgttcc aaatgcgcca cgttccgccg gcatcgcggc
    11821 gggccaggcc gccgccgctc cactcgccgc acccccagag attccccgct ccgtcgagaa
    11881 cgatgtcgta gggattcgtc cgcgggaagg agacatgggg aggcagggga tggacggcct
    11941 tttccccggg ccggcggaaa tggagggcgg tgtcggtcag aatccaaagg gttccatcgg
    12001 cggtccggag cgccttccag acccactcgt tgtccagacc gtcggccgcc cgaatcgttt
    12061 cgagaacccg gggatgcggc cggaccaggt agtggaccgc tccgcgcatc gtccccagcc
    12121 agagcgagtc ggaatcctct cccggagtga tgccgaaaac acaagcgctc ggcaatccct
    12181 ggttgccggt gtggttgatc acggccaggt tgctcaggcg ggccaggccg ccgatgtccg
    12241 tgcccaccca gacgcttttc tcccggtcct caaggaccac gctgacgagg ttcgagggaa
    12301 ggccgttttc gaccccccag tgttcgaaga cggacgcgcc ggggcgcttg agaaacacgc
    12361 cgtcgtcgac cgtggccgcg tacagcgttc ctgacggctg gacggtcagg ctgtatacgg
    12421 cccgtggaac gggggaaccg gttatcttgc gccagtccgc cccgtcccgc agccagacgc
    12481 cggccccggc cgtgcccaac cacagcccct cggcggtgac cgccaaggcc cggaagtccg
    12541 ccggttcgag cccggccgga gaaacgaccg catcgggcgg cgcgtcgccg gaaaagcgcc
    12601 acaagccgtt ggcggccgcg accagaacgc cttcgcggtc ggaaaggatg tcatagacca
    12661 cgggtccctt ctccctgctt ccgggatgga agaggaacca ggtttccccg tcgaagaccg
    12721 ccgggccgtc gtcggttccg acccagatcc gctttcgggc gtcctcggcc aaggcccggc
    12781 agcgaaccat gctcacggcc tgatcgggga ggatttcgag gcgacggccg gcgattcgcg
    12841 agacgccggc cactgtggcg atccacaggg tcccgtcgga cgcgaccagc agctcctgga
    12901 tccgggtgct cggcaggccg tcctcgcgga agaagctttt gaactcagcc ccgttgaagc
    12961 gggccagccc gccccaggtc cccacccaca gataaccctc gcggtcctga gccagggcag
    13021 aaacctgcga ctggggaatc ccctccttca agcccaagac ctcgaacggg agaacctggg
    13081 cgcgggcggg gagggccaaa gcgagaagcg ccgcaacggc ggcggccggg acacgatttc
    13141 tcagtaaata acctcgcatc tattttccct ttttgaaatg aagccgttgc ctgacggccg
    13201 gcggggattg gcggccagga tctcgagggt cgcaggcggt ccggcggcgg ggccgaggga
    13261 caggattttc ttttctccgc ggatcatcgc cgaaaagccc cgtcttgtct tgaaatccca
    13321 tcctgccatc gggaatgagg ataattgagc gtttgaggag ttgtcaagaa aagaaaaggc
    13381 cgtttgccag gaagcccctc tcgcggcagc ggacgccctc ctccatgagc tttgccctcc
    13441 tttacggccc ttggccgtcc tctccgggcc ctgtcctcgc caaaaggccc atctaaaaag
    13501 gattggcccc ttgactaaac cgggcctttt gacgttgcag aaaaccggcg ttttcacttt
    13561 gtctttacag acgaaataaa actcttgaca tccggagcgc gaagccttag attttactcg
    13621 gcgttaatat tattgattat tagtaatatt attatactct tagtatttgt catttgaacg
    13681 ggaggagatg atgagcaaga aactctttcc attcgcggtt ttcatcgcgg tttttctcgg
    13741 ggcggcgttc gccccgggcc agtcggagga cccgaaagcg cgggagctgg cccgcaagat
    13801 cgacgacctt taccggagcg attcgagcta tgcccgcatc gaaatggaaa tcgtcacccc
    13861 cgattggcgg aggacgctgg ccttgaaggg atggacccaa ggacagtcga aaacgctgat
    13921 ccgcatcctg gagcccaaga aggaagccgg gatcgggact ctccgcctgg gcaacgaaat
    13981 gtggaactac ctgccgaaga ccgacaaggt catcaagatt ccgccatcca tgatgatgag
    14041 ctcctggatg ggctcggact tcacgaacga tgatctggtc aaggaattca cgttcgttga
    14101 tgatttcacg ttccgaatgg tgactccgcc ggaggccgaa aagggaattc tctacctgga
    14161 atgccggccc aagccgggag tggccgtcgt ctgggacaag atcgtcatcg ccgtcgctga
    14221 agccgatacg ctcccggtct ggcagaaatt ctatgacgaa aagggccaag tggcccggat
    14281 catgagctac agcgaggtgc gaaccttcgg ccggcggacc gttcccgctg tcatggaaat
    14341 ggttccccgg acgaaaacgg gctgcaaaac cgtcctgcgc taccgggaga ttcaattcga
    14401 tctgtccttg aaggacgatc tcttttccct gcgggcgctg caatccggca agtagaggat
    14461 atcccatgat aaaaatggct ttccgcaata ttttccgcca gaagcggcgt tccctcttga
    14521 ccaccctgac gatgttcggc ggctttgtcc tggcttccct ggccctggga gtcatcgacg
    14581 gaacgtacaa ccggatcgtc gacgagttca ccagaagccg catgggacac atccagattc
    14641 acgcccgcgg ctacctggaa cgcccgaccc tccaaaaagc catccggaac ggccgcgaga
    14701 tcggcgttga aatcgaccgg tcgcgggacg cggaggcgtg ggcccctcgg attatcgccg
    14761 cggccctggc ctcggtcgga gaacggacgg atacggcccg gatcctgggc atcgatccgg
    14821 tccgggagaa cgcggccacc cgctttgaga gaaagatcac ccggggccgg agcttttctc
    14881 cggatccctc gaaggaaatc attctcggcc cgggcttggc ccaggcgctg aaagccgcga
    14941 ttggagacga ggtcgttctg gtttctcaag ccgctgacgg atccctggcc aacgacgtct
    15001 accggctcat cggcctggcc gacaccggcg acatggcgac cgaccggact tccgcctacc
    15061 ttcatctcga ggacgcccgg agcttgttcg ttctcgagga agcggtccac gagatcgtcg
    15121 tcgtttcccg ttccctgaaa aaagcaacga acctgaacgc ctccctccgc gaatccctgg
    15181 ccgcctttcc ggtcgacgtc caaccttggc aggagttcgc caaaagcttt tacaacgcca
    15241 tgcaagccga cagccgcggc catgaaatga tgctcctgat cttgtttatc gtcgtctcgt
    15301 tcggcgttct gaacacgact ctgatgtcgg tcctggagcg gcggagggaa tacggcgtgc
    15361 tgaaggccct ggggacgcgg ccccggcagg tcttccggct gatcctttgc gaagtcctgc
    15421 ttctggccgc ggcgagcgtc gtcttggggg ccggggccgg gtttttaatc aattccgccg
    15481 tcgctcaaca cgagattgtg attttctcca agggggtcac cttcggggga atggagatga
    15541 gatccttcac cacggaggtc aatgctcgga cgttggcgat ccctgccctg accgttcttt
    15601 tctccgcgct tttcgtcagt ctgtggccgg ccgttcgggc cgcccggacc gagccggccc
    15661 gctcgatccg catcttctag gaggaccatc atgagcctct attcgcgact tgcctggcgc
    15721 aatatcttcc gcaacaagcg ccggacctac atcaccggaa tcgccgtcgc cgtcggcctg
    15781 gccgccttga tgttcctgga cgcgctttat atcggcatgg ccgataatat gatcgccgcg
    15841 gccacggctt ccttcaccgg cgaggcgcag atccacggac gggatttcct gctcgcccgg
    15901 gacgcgaacc tgaccgtggc cgggagcgag cggctcctgg ccgacttgag gaacgatacg
    15961 ctggtcaagg cgttcacccc gcggacgctg agccagggaa tggtcacctc cgcggccaac
    16021 gtcaatcccg tcctcctcgt cggggtccgc ccggacaccg agcgggctct cagccagatg
    16081 gacgacgtca tcgaggccgg cgattacttc ggcgggaatg acccgcagaa catcgtcatc
    16141 ggccgcaaac tggccgatct cctcgaagtc ggcgtgggcg accgggtcgt ggtgaccgtc
    16201 tcccgggcgg gaagcgggga gctctcccag gacctgtttc gcgtctcggg catctatcgt
    16261 ttcaacaccg acgatctgga cgacgggttg gccttcgtcc ggatcgaaaa agcccaggac
    16321 atgctcggcc tggccggccg ggtgaatgaa atcgctttgt cgttcaagga tcctcgcgtc
    16381 tcccaggacg agacccatcc tttctggacg gactattcga ccggggggaa cgaagccctg
    16441 ggatggcccc ggcttttccc ggccctgaaa tccgtttatc agatgacgac ggcggccagc
    16501 ctgatcctcg gcttgctgct cttcaccgtg gtcgccctcg gcatcgtcaa taccatgttc
    16561 atgtcgatct acgagcggat gttcgagttc ggcgtcctgc gggcggtcgg aacgaggccc
    16621 ggaggaatcc gccggcttgt gatcctggaa gcgggatcgc tggccctgtt gagcatcgct
    16681 ctcggcgtcg ggctgggatt ccttgtcacc ctgatcgttt ccctgaccgg agtgaattat
    16741 cagaacatcg aattcgccgg ggtgaccatc aaggagctca tctatccggt cttccatctc
    16801 aaacagtacg tcattttccc ggccggcgtg cttctgttca ccctgctggt gggattatac
    16861 ccggccggct tcgccgcccg gatgtccgtc acccgggcca tgaaaagaac gatgtgaagg
    16921 agaacaacca tgaatcccca acaccggcac ctcatcgaaa cggagggact gataaaaacg
    16981 tattcggaca acggggtcca ggtcccggct ctgcggggcg tcgatctgac cattcatgcc
    17041 ggggaattca cggccatcgt cgggccgtcg ggctcgggca agacaaccct gctcaacatc
    17101 atctccggcc tggacaaacc cacgtcgggc aaggtctggc tggacggccg gctcgtctcg
    17161 gacatgagcg gggcggagct gtccgatttc cgccgcgatc acatcggatt catcttccag
    17221 gcctacaacc tcatccccgt cctcaaggtc gaggagaaca tcgagtacat catgcttctt
    17281 cagggcgtgc ccaaggaaga acgccaccgg cgcgtccacg acatcctgcg cgacgtggac
    17341 ctcgaggact atgcccatcg ctatcccaac cggttgtcgg gcggccagca gcagcgcgtg
    17401 gccatcgccc gggccatggt ctctcatccc ttgatgatcc tggccgacga gcccacggcc
    17461 aacctggact cgaaaaccgg cgcggatctt ctggacatga tgcgccatct caatgcggag
    17521 catgggatga ccttcatctt ctcgactcac gaccggatga tcgtcgataa agccaagcgg
    17581 gtgatcgtca ttcgcgacgg gatcgtcgcg agcgatgaaa cccggaattg aggccgcgat
    17641 gagaagaccg cgggcatcaa tcggggccgg agcgctggcc gccgccctgg ccatggcggc
    17701 ccttcccccg gcggcggaag gagagctcaa gctcggcggc tacctgaaaa atcattcggt
    17761 cgtcttccgc ctgcccgaat cgaagacggc cgcttcggac ctgccgctcc cggggatctc
    17821 ccaatttcgc ctccgggtgt tcctcgccgg ggacctgtcg ccggatgtct cgttccaagc
    17881 ggcttacgac ctttcgcccc ggatccagga tacgcttttt tttcgggcct cgccgtttat
    17941 tccgtccccg gaagcgggcg attaccgggc ggccgatttc cgctcgcgcc tcgtcccggc
    18001 gccgggaaag ccggccggaa gcttcggcct ctatcacaac ctcgaccgcc tgtcgctgtc
    18061 ggtcaagatg acgttcgggg atctcatcct cggccgccag ccggtggcct ggggaagcgg
    18121 ccggatcacg aatccaaccg atctcatcgc tccattctcc ttccaggaat tggacaagga
    18181 ggagcgtttc ggcgtggacg ccgtccgcct gaggattccc ctgggcgcct tgagcgagct
    18241 cgacgcgggg gccgttttcg gccgggactt ggagttcaag cggagcgcct ttttccttcg
    18301 gggcaaggtc aatgttctgg agaccgacgc ggccctcctc cttctcggat ttcgcgaaga
    18361 cctgctcctg ggcctcgacc tggcccgctc cttgggcggg gccggcgttt ggctcgaggg
    18421 ggcttgcgtc tttccggacg ccttgaacgg aaaaaagccc aaggcttcaa gctatgcccg
    18481 ccttacggcc ggtatcgacc gcagcctgag cgaccgtgct tatgccttct tcgagtatca
    18541 ttacaactcg gccgggtcct cggagcccgg ggattacacc gccctctttt cccggccggc
    18601 ttatacgagg ggagccgcgt atctgatggg ccgtcattac cttgccgcgg gcttgacctg
    18661 gcaagcgacg ccgctgatcc ccgtttccgg gctcctcctc tggaatgcga ccgacggctc
    18721 gctggcgctt tcgccccagg cggaatacaa tatcgccgaa aatatctacc tcggagccgg
    18781 agcattcgtg ggagtcggcc gtccgccgtt aaggtccccg ttcgatgcaa ccgggaccgg
    18841 gccggtcctt gattcggaat tcggaagcta cccggatttc gttttccttt ctttccgggt
    18901 atatttttga gggaggcttg ccgatgaaaa ccagggcgcg gacgaaaacc ggaacgacgg
    18961 cggcgaagaa ggcccgcaat ccctatccgc cgggcggccg cgaggacgcc atccgccagt
    19021 tcatcctcga acacagcgag catcttttcg tcacccacgg atacggcaag acctccatgg
    19081 acatggtcgc ccgggagtgc ggattgtcca agccgacgct ttacaaatac tttaagaaca
    19141 agtacgagat gttcaccagc ctgtatgagc ggctctaccg gagcctcaat gagatgttca
    19201 agctcatcct cggacggaac cgggacaagg cccaggtcct ggaagagatc atcctcggtt
    19261 actttctctt gatgacctct aaaaaggact ttttgaaaat gtacttccgc gaacagcatc
    19321 tggtcgtcca cgagaacatc gaagaacaca tgtcctggca cgtcgaaagc cgcaaaaaaa
    19381 tggaggagat gctggcccaa tgcctccagg ggatcctccg gccggacatc gccaagcgct
    19441 acggctcggc catggtcgcc tcgaccttgt tcgacatcct ggagggcatg gtttcaaacc
    19501 tcatcctcca cggcgagcag gacccggcca aggaaaaagc gttcatcctg gaatttctcc
    19561 ggtccggcgt tctggcccgg tccgcctgag gaccgcctcc tcttccgcgg cctcgcccct
    19621 ttccccggga ttccgccgtc tccggcttca gtccgtcgtt cattccctcc tctcctctga
    19681 ctcgtgaggg gaaaggatcg atcgggagaa gccaaggcgc tcgtgaaatg aggaaccgcg
    19741 ccggggggcg ccggccttac cgacccggcc ctgggcgaaa cctaccagat cgaagcctgc
    19801 cgcgatgatt atgggtcgcg cggcgggacg aaccaggtcg ccgggacctc ccgggccaag
    19861 aatactgcca atcgcaa
//
LOCUS       DAOOXH010000041        19788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_2621_length_19788_cov_7.211169, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000041 DAOOXH010000000
VERSION     DAOOXH010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 19788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19788
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2621_length_19788_cov_7.211169"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..509
                     /locus_tag="PKV64_06350"
     CDS             141..509
                     /locus_tag="PKV64_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN16737.1"
                     /translation="MARKIVVIDDDKVTRKLLTELLNRNGYEAYGAHDGMEGVALVKW
                     EQPELVLTDLLIPLLDGIGVCQQIKSDPELSGIKVIVMSALKNPLLVREARAVGADDF
                     IDKPIRTNTLLELIARLLQN"
     gene            complement(683..3565)
                     /locus_tag="PKV64_06355"
     CDS             complement(683..3565)
                     /locus_tag="PKV64_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPN16738.1"
                     /translation="MKILRPAFALLLLVFLAVLSGGVPAAGQEAPAPAQPAGPRPIEL
                     RDILAWRTIVSAVVSDNGRWFAARLAPQEGNGEVVFKEIRGDKAYAFPAGEGRKGAVA
                     ISADGAFGAFAVNPEPEEARRLRKERAPLFAGAAVVNLASGEKTVYDKIKSFSFPAEN
                     PAWIALHKTAPEGQAKEKDKGTGSDLILRELATGRELVLGNVSEFAFDKKGARLALLI
                     DAVGQAGNGLLLRDMTTGVLAVLDSGKASYQRLSWTENGGALACLKGREDKDYEDKLF
                     SLIGFTFPAGGPRKHAYDPQDDKSFPAGLTISPNRTPRWTESLDALLFGIHAPKKKSA
                     GSGPGPVAPVKPGEEPDENIPDLVLWHFNDPRLQSQQQVEAERDRNFSYLSVYRIKEN
                     KFIRLADEDVRDVRPAPKDRFALGRDVRAYELDGSLDGRRYEDLYVIDMISGTRTLAL
                     KKTRWPVLPSPDGTKLLHYAGGVYSVYDMAAAVSRPITRDVPADFVNEDDDHNEIDPP
                     DPPVGWMKDSRSVLLSDGWDVWSVPVQGGPSLNLTGNGRKEGLRYQRLRLDPDEKGID
                     PARPIYFGTYGEWTKKAGFSVIEKGSPGPRVLVWEDAAFGPLLKARLADVYLTTRETF
                     RDFPDWRAAGADLKSWIRLTEANPQQKDFLWSSGVRLVDYVIDPKSGKTNRLQGALFL
                     PAGYEPGRSYPTIVYYYEKMSQMLNRHLPPASNGFNKTVYTSNGYAVFFPDITYKIND
                     PGMSAAWCVLPALDAAMATGVVDRAKVGLQGHSWGGYETSFLVTQADFAAAVAGAPLT
                     NMISMYSSIYFNSGVSNMAIFESSQGRFKGGYWDNLEAYQRNSPVYHASAVRTPLMIL
                     HNDKDGAVDWNQGIEYFNTLRRLKKTVVMLQYTGENHGLAKPANQRDYTVRMKEWFDH
                     WLKGSPAPDWLKEGVPHLNLKDHLRERSKLWRPEEKKEPEKGEGKK"
     gene            3935..5287
                     /locus_tag="PKV64_06360"
     CDS             3935..5287
                     /locus_tag="PKV64_06360"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HPN16739.1"
                     /translation="MKSVFRWAAALLIVSLASAAGASGQPNPAASSFASKPYEAISLF
                     GRPLVSPAPSPAAVKSYEDALKDFDVDPSEDNWIWLGRRTAYLGRFREAIAVYSEGLK
                     KHPDSCRLYRHRGHRYISIRQFGRAISDFEKAAALAAGTPLEVEADGNPNPSGIPVSN
                     TQFNIYYHLGLAYYLTRDFVKAEAAFRECLKWSNNDDSVVAATDWFYLILRRTGKADE
                     ARALLSKINSRMKLLENGAYLERLLMFKGEIETDDFGRGRSGETAIETAIRSYGLGMA
                     RLWTGDKTGARRLFEEMLRREDWASFSVIAAEAELADLVRAEPDRSSPGATLAAWTLS
                     WNVYDLDLTQKLFEPSPRTTYFSSEKAGRIQGMDALMDHSRSFGFIPGGKTADNRLWL
                     DGLDVLGVEDMALVTAFWYFDRDVAGPGPVQKGPVTFAMMREGGNWLIVHAHFANDPR
                     "
     gene            complement(5413..6723)
                     /locus_tag="PKV64_06365"
     CDS             complement(5413..6723)
                     /locus_tag="PKV64_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HPN16740.1"
                     /translation="MTVYNLVSFAGIFVLAGVAWLFSSNRKIVNVRVLFWGIGLQLLV
                     ACFVFVIPAGTKAFLWVNDAVTTVLSAATAGTKFVFGRLALPPGATNEAGETSLGYFL
                     AFQGLPTIIFFAALLGALYYLRIMPFFVRLFARLFSRSMKISGAEALSVSANIFVGVE
                     SALVVRPHLKDMTRSELGTIITAGMGTIASTVLAMYVMMLQKSIPTIAGHLVSASFIS
                     APASIVMAKLLMPETEKPLTLGLDVKPHYEREDNLIMAIINGAGEGLKLLGGIVTLLI
                     AFLGILALVNLGLGWAGGLVNGLFGWHFDWSIEALLGYLFTPLTLAMGVVPADAFSIA
                     KILGDRTILTEVFSYGRLSDLLAAGALKDPRSAIIASYALCGFAHVASLAIFVGGTGA
                     LAPTRIRDISRIGFRALLAATLACLMTGAVAGTFYTGSSVLFGR"
     gene            6940..8538
                     /locus_tag="PKV64_06370"
     CDS             6940..8538
                     /locus_tag="PKV64_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN16741.1"
                     /translation="MRNAALALALSFLLVSCTSSPRRAVRTAVPPADDMKTAETASAL
                     AGRGSYVFLKDACRIYGGLYAKAGLRERVAAPYFRAVFLLFLRERELGIGGTETFARA
                     ERLAGENPGLTGYRNWLAAAREIPLRVKGVVRDSIPWLENTFDPEELDRLRADLYARA
                     PADGEAAYFYLALACPGKSSYDRADDRAAILAAHPGSIPILFRTGFCPAPAAGRFDEV
                     LQADPEFWEAYGFRGEAALGRGEILSAEKDLLTAFARIPESALYPVLLASLYFFTEEF
                     EKSILYCEKALALLPEYRDALLIKAIGLSQLGRNTDAIGVLNRIIEMRYYLQGEAHYW
                     LAWNYHALNERDSARGHIEAAKGPMPTNTHVFSLSGTLALEANEPAAAEADFQQAVEF
                     DGANDEALLGLARIAERRERWTDAAGWYERTITAMEGKAEGLKAKIEEIAGSGLAAER
                     KAGMLARRESQIRTAEIVAAAAAFNAAVAWANAGDTAAARAAAERAAGHPRFKERAAE
                     LLQRIKGAPAPSPSRKNDGQGEGV"
     gene            complement(8721..9515)
                     /locus_tag="PKV64_06375"
     CDS             complement(8721..9515)
                     /locus_tag="PKV64_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16742.1"
                     /translation="MRRALLWILAVVLTLAAAVYQRISGPTYPLRGKVEVSGTEVPYR
                     LPRSAETGRDLEIPVNNAEGRLSGFIEFKRHPTEDPWTRVEMVPAGDVLSAELPAQPP
                     AGKLAYRIVLSKDGREMPLAGGESVIVRFRGAVPPLIQIPHIIVMFLAMLFSVRAGLA
                     ALNRREHPRKLVLATCALLFLGGFILGPLMQQYAFGKLWTGFPFGTDLTDNKTLVAMI
                     AWLAALWAGRKGRAARGWVLAASLILLIVYSIPHSLLGSELDYTKL"
     gene            complement(9530..10234)
                     /locus_tag="PKV64_06380"
     CDS             complement(9530..10234)
                     /locus_tag="PKV64_06380"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HPN16743.1"
                     /translation="MSAENRRSDPGRIFWGLVLILIGALVLFLRYDFWDFGYLLSTWW
                     PSILILIGISVLIGNRFRRWGAGLILILFGGLFQAWELGYLEVDVWRKIWPAVLIVLG
                     AWIIFRPSRRRQDRFRAAPAPGGDIDVTAVFSGAKRRVEARNFKGGEATAVFGGVDLD
                     LTGAGLEGGQATMEATAIFGGVDIIVPRDWKVVLDGTPILGGVSMKHANPPESEVQST
                     LHVRGTAIFGGVTVKD"
     gene            complement(10311..11513)
                     /locus_tag="PKV64_06385"
     CDS             complement(10311..11513)
                     /locus_tag="PKV64_06385"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPN16744.1"
                     /translation="MANTKSKKGVIGILTGGGDVPGLNPAIRAVTLRALSEGYKVIGL
                     KRGWAGLMEMVRDKEADNTAHYCLLTEEYVRKAGRTGGTFLHTSRTRPSHVPQAAVPA
                     HLREAYKEKTNDLTPDILKNLDYLGIDYLVPIGGDDTLSYGVHLHQQGVKVVAVPKTM
                     DNDVPGTDYCIGFSTCVTRTIALSNALRTSAGSHERFLVIEVFGRYAGFTALLPTLAA
                     AADRCLIPEHPFDMERLAELLSQDRAENASRYAIVIVAEGATPAGGDMVFCDHETDMF
                     GHKKLGGIGDLVSQKLKELAPKYNNGRKVDVLNQKLGYLVRCGEPDALDYIIPMAFGN
                     IAFDLIQEGKSGRMACLRKGEYDDVPLEVVTSRKKIVDIDTSYDRDRLRPLYSHFSRK
                     PFLLIEGA"
     gene            11815..12435
                     /locus_tag="PKV64_06390"
     CDS             11815..12435
                     /locus_tag="PKV64_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HPN16745.1"
                     /translation="MLEAMQEALIGGRKADVEALVDRAIAGGLPTRRILNEGLIAGME
                     KLGELFKNNEVFIPEVLVAARAMNAALVKLEPLLIKEGIEPRGCVVIGTVKGDLHDIG
                     KNLVAMMLKGAGFKIIDLGADVAADKFVEAAKSNEADVIALSALLTTTMVQMKTVVAA
                     LEAAGLHVPVIVGGAPLTADYASQIGARGYAPDAASAVDVVHRLVG"
     gene            complement(12490..14517)
                     /locus_tag="PKV64_06395"
     CDS             complement(12490..14517)
                     /locus_tag="PKV64_06395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HPN16746.1"
                     /translation="MPRSPVLVPLLLFIVLLLLASAAGWIYYSQSKEQILATAAANLN
                     AVGNLKVSLLDGWRRERLRDAAIISANPFNQAMIIPFLEADPAVSTADPRIKAWLDSL
                     VKAKGYMDAVLLDTDGRVRMASGSSGPVIGTNAEETIDRVLATGEPYLSGFHTSSEIG
                     LKAHLDLYAPVSGRGPDGKVSCDGIFLFRIDPADFLIPTMRSRGAPGRSVETLLVRRE
                     GDDILFLQDPRFRPDSGLKLRLPAAEADLPASMAEREKNGAFEGTDYRGVRVLSALKA
                     VPGTSWRVVAQEDLSEILEPLLARMSSFVSMIIILCFGTGVMLLFWIKRREAAYFRKQ
                     YEIEHDRLALVQHFEYLHKYANDIVILADRDHRVIEANDRALAVYGFSRAELIGFRVP
                     DLRPWEARADFPRHLEEAEKTGSLIFETLHRDKDGIAFPVEVSLRLIDIGGARYHQAI
                     IRDIRERKRAEKRILDALREKEVLLREIHHRVKNNMQVISSLLRLQSARFTEAEVREA
                     FLESQGRIKSMALVHEKLYMTKDLSRIDFADYIKSLTSTIFASHEEDGRIALKLDLDK
                     VYLDINTAVPCGLILNELILNALKHAFPNNRRGRIRVELRENEKEIIRLTVRDDGIGL
                     PEGCKPGKVESLGFQIIALLAEQLRGWIDVRREGGAAISLWFKLPSVPETN"
     gene            complement(14659..15561)
                     /locus_tag="PKV64_06400"
     CDS             complement(14659..15561)
                     /locus_tag="PKV64_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPN16747.1"
                     /translation="MFDSLMQTPHIGEILSLASAVVWAVSVILFRIAGDTIHPLVMNL
                     FKNVLALVLLGGTVLFIGGAPDLPPRIFALFVLSGILGIALSDWLFFTALVRLGAELT
                     AIVDCAYSPFVIGLSFLFLGERMNRLQIAGVGLILGAVILITRKKTDERLSRRALLSG
                     VGIGIVSMLLTAGGIVLVKPFLGGISVVWATLIRMTGGAAAGLVLWALHPRRRELLRP
                     LRAGANYKLLIPASVLSAYFSVLIWMAGMKYTLASVASALNQLNTIFVFLLAAVVLKE
                     KITPLKAAAVALAFAGAILVSIRF"
     gene            15838..16863
                     /gene="rlmN"
                     /locus_tag="PKV64_06405"
     CDS             15838..16863
                     /gene="rlmN"
                     /locus_tag="PKV64_06405"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPN16748.1"
                     /translation="MRDIRDLSFDELRAVLAEDGEPAYRAEQVFAWLYRRGASSFAEF
                     TDLPRPLRDRLAARFSLRPLEAVEIRCSADGTTKTLFRLSDGWNIETVFIPSGTRRTI
                     CLSTQAGCKFSCAFCASGKGGFHRHLTPGEILGQVLHFRSGPDAGLTNLVFMGMGEPL
                     DNLDNVERVLRVLNDPRGFGFAARRMTVSTCGLVPGIERFARIDLQINLSISLHAADD
                     ETRNRLMPVNRRYPLAELISACEDYLAAGGRKITFEYVLLKGVNDRPADAERLARLAR
                     RLGAKVNLIAFSPVEGLPFERPDPEAASAFAGRLRNKGTAVTVRSSKGSDIQAACGQL
                     AGSPAAG"
     gene            complement(16868..18175)
                     /locus_tag="PKV64_06410"
     CDS             complement(16868..18175)
                     /locus_tag="PKV64_06410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPN16749.1"
                     /translation="MTFEKGTRIRLSIEKIVYPGRRLARHEGRVYFTDEGLPGETVEI
                     EILKERASFVEARTTAVLSPSPRRIPARCGHFSACGSYQVLPYSEQLDLKKSQLREMF
                     RRLGTGPFFEPEVTASPLEWNYRNKIRFSVRGSGPDVFLAYHSPGSRAAFVPTDGRCH
                     LASEASCAAARTVLEAVRDGGLTDLEDVEARESRTEGTLLLNLFWRKRRPEKAAAAVR
                     DALAARFPVSGLVSWAPGRSGRSLPFLEDGREILLERAAGLTFEIGAGSFFQVNGAIL
                     PLVLEAVSEAAGLRGREIVADVYSGVGTFGLALARSAARVFVIESFPDNVRRLEANIV
                     RNGLGNVEVLDGSAEAWMKDLAGRGLDTVILDPPRKGLGPAVVRALIERPAGKIIYLS
                     CNPATLIRDLGLLGAAYAPASVRLFDFFPQTPHIETLAVLVRK"
     gene            complement(18258..18884)
                     /locus_tag="PKV64_06415"
     CDS             complement(18258..18884)
                     /locus_tag="PKV64_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPN16750.1"
                     /translation="MFLLVGLILGFVAAIPLGPVNAFVISQSMKRNFFHGFVAGATAA
                     VLDFLYCLISLLGIAFITVNITRFQIPLKIAAGIIMLIIGWRLYLTSKKPVIPKQEKL
                     PTSFTPRPILGVILLYVSNPSLYFFWIWAAGWAAGHGWILNYAAAPYLFAASCGLGGF
                     IWYTVVSYYVSHHHHQFKPRTFQRIFLILAIVMTGFAVYTFAGIFLHF"
     gene            complement(19133..>19788)
                     /locus_tag="PKV64_06420"
     CDS             complement(19133..>19788)
                     /locus_tag="PKV64_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPN16751.1"
                     /translation="VADKAVDAKQTGSGFTPILSLHYSPIPELNLSARYEFNTKIELT
                     NKTTVDGTGLFPDGQKTHGDIPGFLALGADYGISPDFRASLSFSYYFDKNADWDGRED
                     LLDKNSIDLSFGLEYDISDLIMVSAGYQLSRYGLVDDYQSDMSYDLNADTFGFGGRIR
                     LSPNFNVDLGALIVNYKDYSKNLPYPALGASFRELYQQTTFVFSIGASYHFGGAAQE"
BASE COUNT         3775 a         5968 c         6494 g         3551 t
ORIGIN      
        1 tccgcagcgt ctttaacata acgaatatat tatataacga aattataata tgtcaaggga
       61 gccccgcgtt ttcgtcctgc cggcgtccac tggaatatct ttctctgttt atgtaaaatg
      121 atcctccggc ggggaacgtc atggctcgaa aaatcgtggt gatcgacgac gacaaggtga
      181 cgcggaagct tttgaccgag ctgctgaacc gcaacggcta tgaggcttac ggggctcacg
      241 acggcatgga aggcgtcgcc ttggtgaaat gggagcaacc cgagctggtt ttgaccgatc
      301 tgttgatccc ccttcttgac ggcatcggcg tctgccagca aatcaaatcc gatcccgagc
      361 tcagcggaat caaggtcatc gtgatgtcgg ccctgaaaaa cccgctcttg gtccgcgaag
      421 cccgggccgt cggggcggat gatttcatcg acaaacccat ccggacgaat actttgctcg
      481 agctgatcgc ccggctgctt cagaattgag gccccgcggc ccctcttgcc ttttgacttt
      541 tgcttgactt cgtactattc tatccgcgga tgctggctgg agaggatctt tcaagccccg
      601 cttccttttc aaaccttggc gcctcgacag ccagcgcctc tattttacgg ggcatggggc
      661 gccataaagc cgccgttcgt tcttatttct tcccttcgcc tttttccggt tccttctttt
      721 cctcgggccg ccagagcttc gaccgctccc gcagatggtc cttgagattc aggtggggga
      781 cgccttcctt cagccagtcc ggggcgggcg agcccttcag ccagtggtcg aaccattcct
      841 tcatccggac ggtgtaatcc cgctggttgg ccggcttggc caggccgtgg ttctctccgg
      901 tgtactggag catgacgacc gtctttttca accggcggag cgtgttgaaa tactcgatcc
      961 cctggttcca gtcgacggcc ccgtccttgt cgttgtggag aatcatcagg ggcgtgcgga
     1021 cggcggacgc gtgatagacg ggggaattgc gctggtaggc ctcgaggttg tcccaataac
     1081 cgcccttgaa ccggccttgg ctgctttcga agatggccat gttggaaacg ccggaattga
     1141 aatagatcga gctgtacatg gagatcatgt ttgtcagcgg agccccggcc acagccgcgg
     1201 cgaaatcagc ctgggtcacc agaaaagacg tctcgtatcc gccccatgaa tgaccctgga
     1261 ggccgacctt ggcccggtcg accaccccgg tcgccatggc cgcgtccaga gctggaagga
     1321 cgcaccaggc ggcgctcatc ccgggatcgt tgatcttgta cgtgatatcc gggaaaaaga
     1381 cggcgtatcc gttgctcgtg tacaccgtct tgttgaagcc gttggacgcg ggcggcagat
     1441 ggcggttgag catctgggac attttttcat agtaatagac aatcgtcgga taagaccggc
     1501 ccggttcgta tccggccggg agaaagagcg cgccctggag ccggttggtt tttccgctct
     1561 tcggatcgat gacataatcg accagcctta cgccgctcga ccagagaaaa tccttctgtt
     1621 ggggattggc ctcggtcagg cggatccagg atttcaggtc cgccccggcg gcccgccagt
     1681 ccgggaaatc ccggaacgtt tcgcgggtgg tgagatagac atccgctaga cgggccttca
     1741 ggagaggtcc gaaggcggcg tcctcccaga cgagaacgcg cggcccgggc gatccctttt
     1801 cgatcacgga gaatcccgct tttttcgtcc attcaccgta cgttccgaag tagatcggcc
     1861 gggcgggatc gatgcccttt tcgtcgggat cgagacgcag acgctgatac cggaggccct
     1921 ctttccggcc gttcccggtg aggttgaggc tcggcccgcc ctgaacgggg acggaccaga
     1981 catcccagcc gtccgagagg aggacggacc gggaatcctt catccagccg acggggggat
     2041 cgggcggatc gatttcattg tggtcgtcgt cctcgttgac gaaatcggcc gggacgtccc
     2101 gggtgatcgg ccgggagacg gccgcggcca tgtcatagac ggaatagacg ccgccggcgt
     2161 agtggagcaa cttcgtcccg tcgggggacg gcaggacggg ccagcgggtt ttcttcaagg
     2221 ccagggttcg tgttcccgag atcatgtcga tcacgtacag gtcctcgtac cgccggccgt
     2281 cgagggatcc gtccagctcg taagcccgga cgtcgcggcc gagggcgaag cggtctttcg
     2341 gcgccggacg gacgtcgcgg acgtcttcgt cggccaggcg gatgaacttg ttttccttga
     2401 tccggtagac cgacaggtaa ctgaaattcc tgtccctctc ggcttcaact tgctgctggg
     2461 attgaagccg cggatcattg aaatgccaga ggacgaggtc cggaatgttt tcgtccggct
     2521 cctctccggg cttaaccggc gcaaccggcc ccgggccgga cccggcgctt ttcttttttg
     2581 gcgcatgaat gccgaacagg agagcgtcca ggctttcggt ccagcgcgga gtccggttcg
     2641 ggctgatcgt caggcccgcg gggaagctct tgtcgtcttg cggatcatag gcgtgctttc
     2701 tcgggccgcc ggccggaaaa gtgaagccga taaggctgaa cagcttgtcc tcgtagtcct
     2761 tgtcttcccg gcccttgagg caggccagag cgccgccgtt ttccgtccag gaaaggcgtt
     2821 gataagacgc ctttccggaa tcgagaaccg cgagaacccc ggtggtcatg tcgcggagaa
     2881 ggagcccgtt gccggcctgt ccgacggcgt cgatgaggag agccaaccgg gcgcctttct
     2941 tgtcgaaggc gaactcgctc acgttgccga gaacgagttc ccggccggtg gccagctcgc
     3001 ggagaatgag gtcggagccg gtccctttat ccttttcctt ggcctgtccc tccggggcgg
     3061 tcttgtgaag ggcgatccag gccggattct cggccgggaa agaaaacgac ttgattttat
     3121 cgtagacggt cttctccccg gaggccagat tcacgacggc cgctccggcg aagaggggag
     3181 cgcgttcctt ccggaggcgc cgggcttcct cgggctcggg attgaccgcg aaggcgccga
     3241 aggcgccgtc ggccgaaatc gcgaccgcgc ctttccggcc ttccccggcg gggaaggcat
     3301 aggctttgtc cccccggatt tccttgaaaa cgacttcccc gtttccttcc tggggagcga
     3361 ggcgggcggc gaaccatcgg ccgttgtccg agacgacggc cgagacgatt gtcctccagg
     3421 cgaggatgtc acggagctcg atcggccgcg gcccggccgg ttgggccgga gccggggctt
     3481 cttggcccgc ggccggaacg ccgccggaaa gaaccgccag gaaaacgaga agaagaaggg
     3541 cgaaagccgg gcgcaggatt ttcatggagc ctcccgaaaa gattggatgt ctttctggca
     3601 aaggaagtca tggtaaggga aagaccggtg gttttcaagc ccgcgaatcg cgccgtttaa
     3661 aatcctaatc cgattggaaa aggatatccc ggatgattat caggccggtc ggcccgtctc
     3721 catcgtcttg tcgtcccggg atagatcttg acttccccgg atcgacgatt tatcctccgc
     3781 cgcggaagat cattgattcc aaagaaatcc cggccgctcg cttaagccgg ggatcgaaag
     3841 cacctccatc ggcggggcaa agccccgccg gccgtcttca ggcgaaaaat cgggcttgaa
     3901 gaaaacggcc gggcggacgg gaaggagagt ctcgatgaaa agcgtgttcc gatgggccgc
     3961 ggcccttttg attgtcagcc tcgcttcggc ggccggcgcg tccggtcaac cgaatcccgc
     4021 cgcctcctcc ttcgcctcta agccctatga agcgatttct ctgttcggcc gtccgcttgt
     4081 ttccccggcc ccttccccgg cggccgtgaa aagctacgag gacgccctca aggatttcga
     4141 cgtcgatccg agcgaagaca actggatctg gctcggccgc cgcacggcgt acctcggccg
     4201 cttccgcgaa gcgatcgccg tctacagcga aggcttgaaa aagcatcccg attcctgccg
     4261 tctgtaccgc caccgcggcc accgctatat ttcgatccgc cagttcggcc gggcgatctc
     4321 cgatttcgag aaggcggccg ccctggccgc cggcacgccg ttggaggtcg aggccgacgg
     4381 aaatccgaat ccgtcgggca ttcccgtttc caacacccag ttcaacattt attaccatct
     4441 cggcctggct tattacctga cccgggactt cgtcaaagcc gaagcggcct tccgcgagtg
     4501 cctcaaatgg tccaacaacg acgactccgt cgtggccgcg accgactggt tttatttgat
     4561 tctgcgtcgg acgggaaagg cggatgaagc ccgcgccctc ctgtccaaga tcaattcgag
     4621 gatgaagctt ctcgagaacg gagcctatct cgagcgtctt ttgatgttca agggagagat
     4681 cgaaacggac gatttcggcc gcggccggtc cggcgaaacc gccatcgaaa cggcgatccg
     4741 ttcttacggc ctgggcatgg ctcgtttatg gacaggcgac aaaaccgggg cccggcggct
     4801 cttcgaggag atgctccgcc gcgaggactg ggcgtcgttt tccgtgatcg ccgcggaggc
     4861 ggaactggcc gatcttgtcc gggccgagcc ggacaggagt tcacccggcg cgacgctcgc
     4921 ggcttggacc ttgagctgga acgtgtacga cctcgacctg actcaaaagc ttttcgagcc
     4981 ttcgcccagg acgacttact tttcctccga aaaggccggc cggatccagg ggatggacgc
     5041 cctgatggac cattcccgga gctttggctt cattcccggg ggaaaaaccg cggacaaccg
     5101 cctctggctc gacggtctcg atgtcctggg cgttgaggat atggccctgg tcacggcctt
     5161 ctggtatttc gaccgggacg tggccggccc gggccccgtc caaaaaggtc cggtgacgtt
     5221 cgccatgatg cgggagggcg gaaactggct gatcgtccac gcccatttcg ccaacgatcc
     5281 ccggtgaaac cggaggagcg ggcttttatt aagcaagcgc cggggccggg gcttgttccc
     5341 cggggacatt ccgaagggtg ctcatccccg gcggaaacga acaggatgac gtcagcccgg
     5401 ccgggcggtc tctcatcgtc cgaataaaac cgacgagccc gtataaaacg tcccggccac
     5461 ggccccggtc atgaggcagg ccagggtggc ggcgagcaaa gcccgaaatc caatccggga
     5521 gatgtcccgg atccgcgtcg gggcgagcgc gcccgtcccg ccgacgaaga tcgccaggga
     5581 ggccacgtgg gcgaagccgc agagggcata ggaggcgatg atggccgagc gcggatcttt
     5641 gagcgcgccg gcggccagga gatctgacag gcgcccgtag gaaaaaacct cggtcaagat
     5701 cgtccggtcg ccgaggatct tggcgatgga gaacgcgtcg gccggaacga cgcccatggc
     5761 cagggtcagg ggggtgaaga ggtagccgag gagagcttcg atggaccagt cgaaatgcca
     5821 gccgaagagc ccgttgacca ggccgccggc ccatccgagg ccgaggttga ccagggccag
     5881 gattccgagg aaggcgatga gcagggtgac gatccccccc aggagcttca ggccttcccc
     5941 ggcgccgttg atgatcgcca tgatcaggtt gtcctcgcgc tcatagtggg gcttgacgtc
     6001 gagccccagg gtcagcggct tctccgtctc gggcatgaga agcttggcca tgacgatcga
     6061 ggccggagcc gagatgaacg aggcggagac gaggtggccg gcgatcgtcg ggatggattt
     6121 ctggagcatc atcacgtaca tcgccaggac ggtcgaggcg atcgtgccca tgccggcggt
     6181 gatgatggtc ccgagctcgg accgggtcat gtccttgagg tggggccgga cgacgagggc
     6241 cgattcgacg ccgacgaaga tgttggccga aacggagagc gcctcggccc cgctgatttt
     6301 catcgaccgg gagaagagcc gggcgaacaa ccggacgaag aacggcatga tccgcaggta
     6361 gtagagggcg cccaaaaggg cggcgaagaa aatgatcgtc ggcaagccct ggaaagccaa
     6421 aaaatagccg agggaggttt cgccggcttc gttcgtcgcg cccggcggca gggccaggcg
     6481 gccgaagacg aacttcgtcc ccgccgtggc ggcgctcaaa accgtggtga cggcgtcgtt
     6541 cacccacaga aacgccttgg tcccggccgg gatgacgaag acgaagcagg cgacgagaag
     6601 ctggagcccg atgccccaga agagaacccg gacgttgacg attttccggt tcgacgagaa
     6661 aagccaggcg acgccggcca ggacgaagat gccggcgaag ctgacgaggt tataaaccgt
     6721 catgcctttc ctccttcagg tctcgagccg ccgcgggccg aaatctcctt gatgtcccgc
     6781 ggccgagtgt aatcagccgc agaagggatg tcaaatgacc gaacggcgcc gttaaaaatc
     6841 ttaccctcct ccgcttgtcc cccggtttct tgattccggc cggcgatcgg attacactga
     6901 atccgcgaag agagcggaag gatcggagaa aggattccga tgagaaacgc ggctctcgct
     6961 ctcgcgcttt ccttccttct cgtttcctgc acctcgagcc cccgacgcgc cgtccggacg
     7021 gccgttcccc cggctgacga catgaaaacg gccgagaccg cctcggcttt ggccggccgg
     7081 ggatcgtacg tcttcctgaa ggacgcctgc cggatatacg gcggcctgta cgccaaggcc
     7141 ggcctgaggg agcgcgtcgc cgccccgtat ttccgggcgg tttttctcct tttcctccgg
     7201 gaacgggagc tgggcatcgg tggaaccgaa accttcgccc gggccgagcg tttggccggg
     7261 gaaaatcccg gcctgaccgg gtaccggaat tggctggccg cggcccggga aatcccgctc
     7321 cgggtcaagg gggtcgtccg ggactccatt ccctggctgg aaaatacctt cgatccggaa
     7381 gaactcgatc gccttcgggc cgacctgtac gcccgggctc ccgccgacgg cgaagcggcg
     7441 tatttttacc tggccctggc ttgtcccggg aaatcttcct acgaccgcgc cgacgaccgg
     7501 gcggcaatcc tggccgcgca tcccggttcg atcccgatcc tgttccggac gggattctgt
     7561 cccgcgccgg cggccggacg attcgacgag gtcctccagg ccgacccgga attctgggaa
     7621 gcttacggct tccggggaga ggcggccctg ggacgggggg agatcctaag cgcggaaaaa
     7681 gacctcctga ccgccttcgc ccgcatcccc gaatcggcct tgtatcccgt tctgctcgcc
     7741 agcctctatt tttttaccga ggaattcgag aaaagcatcc tctattgcga gaaggccctc
     7801 gccctgctcc cggaataccg cgacgccctt ctgatcaaag cgatcggcct ttcccagctc
     7861 ggccggaaca cggacgcgat cggcgttctg aaccggatca tcgagatgcg ttactacctc
     7921 cagggggagg cccattactg gctggcttgg aattatcatg ccttgaacga gagggactcc
     7981 gcccgggggc atatcgaggc ggcgaagggg ccgatgccca cgaacacgca tgtcttcagc
     8041 ttgtccggaa ctctggccct ggaagcgaac gaaccggccg cggccgaagc cgatttccag
     8101 caggccgtgg aattcgacgg ggccaacgac gaggccttgt tgggcttggc ccggatcgcc
     8161 gagcggaggg aacggtggac ggatgcggcc ggctggtacg agcggacgat cacggccatg
     8221 gaggggaagg cggaggggtt gaaggcgaag atcgaggaaa tcgccggctc ggggctggcc
     8281 gctgaacgca aggcgggaat gctcgcccgg cgcgagtcgc agatccggac ggcggaaatc
     8341 gtggccgcgg cggccgcttt caacgccgcg gtcgcatggg ccaatgccgg cgacacggcc
     8401 gcggcccggg cggcggccga acgggcggcc ggacatccgc gattcaaaga gcgggccgcc
     8461 gagctccttc aaaggatcaa aggcgcgccg gcgccctcgc cctctcggaa gaacgacggt
     8521 cagggagagg gtgtttgacg ggaccgcgga tgtccggact tcagccgtaa atccggcgaa
     8581 tccgcccttc ttcaagatcg aattgcggcg accaaggcct cggacaagga tgtcgaggcg
     8641 cgacgccgcc ggatccgtgg cgccttcaac cgggaatcgg cggctttctt ctccgccatg
     8701 ggccggttca aattgaagag ctagagcttc gtatagtcga gctccgagcc gagaaggctg
     8761 tgggggatgg aatagacgat caagaggatg agcgaagcgg ccaggaccca gccccgggcc
     8821 gcccgtccct tccgcccggc ccacaaggcc gccagccagg cgatcatcgc gaccagggtt
     8881 ttattgtccg tcaggtcggt tccgaacggg aatccggtcc agagctttcc gaaggcatac
     8941 tgctgcatca gcgggccgag gatgaatccg ccgagaaaga gaagggcgca ggtcgccaag
     9001 acgagcttcc gggggtgttc cctgcggttc agggcggcca ggccggcccg gaccgaaaac
     9061 agcatcgcca ggaacatcac gatgatatgg gggatctgga taagaggcgg gacggcgccc
     9121 cggaaccgga cgatgacgga ttctcctccg gcaagcggca tctcccggcc gtccttcgat
     9181 aagacgatcc ggtaggcgag cttgccggcc ggcggctggg cgggaagctc ggccgaaagg
     9241 acgtccccgg ccgggaccat ctccacccgc gtccatggat cctccgtcgg atgccgcttg
     9301 aattcgatga agccggaaag ccgtccctcg gcgttgttca ccgggatttc caggtcgcgg
     9361 cccgtttccg cgctccgggg aaggcgatag gggacttccg tcccggagac ttcaaccttt
     9421 ccccggaggg ggtaggtcgg tccggatatc cgctggtaga cggccgcggc cagggtcaaa
     9481 acaaccgcca ggatccagag aagcgccctt ctcatgatga cggatccggt caatccttaa
     9541 cggtgacgcc gccgaaaatc gccgtccccc ggacgtgcaa ggttgattgg acctcggatt
     9601 caggcggatt cgcatgcttc atggacaccc cgcccaggat cggcgttccg tccaggacga
     9661 ccttccaatc ccgggggacg atgatgtcca ctccgccgaa aatcgccgtc gcttccatgg
     9721 tcgcttgtcc gccctcgagg ccggccccgg ttaaatccag atcaaccccg ccgaatacgg
     9781 ccgttgcctc tccgcccttg aaattccggg cttcgacgcg gcgcttggcc ccggaaaaaa
     9841 cggccgtcac gtcgatatcg ccccccggcg ccggggcggc ccggaaacgg tcctgccgcc
     9901 ttctcgacgg ccggaagatg atccacgcgc cgagaacgat caagaccgcc ggccaaattt
     9961 tccgccagac gtccacttcg aggtaaccga gctcccaggc ctggaacaag cccccgaata
    10021 ggatgaggat gagtccggcc ccccagcggc ggaagcggtt cccgatcagg accgaaatcc
    10081 cgatcaggat caggatcgac ggccaccagg tggacagcag gtatccgaaa tcccagaaat
    10141 cgtagcggag gaacaagact agggccccga tcaggatcag gaccaagccc cagaagatgc
    10201 ggccgggatc ggaacggcgg ttttcagcgg acatggcgtc ctccttcttc agcatttgcg
    10261 gcgaatgata tcacagccgg accgccggag aaacggcgtc cggtccggac tcaagcgcct
    10321 tcgatcagca ggaacggctt tcggctgaaa tgggagtaga gcgggcgcag ccggtcccgg
    10381 tcataggacg tatcgatgtc gacgatcttc tttcggctgg tcacgacctc cagcggaacg
    10441 tcgtcgtatt cgcccttgcg gagacaggcc atccggccgg acttgccttc ctggatcagg
    10501 tcgaaggcga tgttgccgaa agccatggga atgatgtaat cgagcgcgtc cggctctccg
    10561 caacggacga ggtagccgag cttctggttg aggacgtcga ctttccggcc gttgttgtat
    10621 ttgggggcca gctccttgag cttttgggac accaggtcgc cgattccccc gagcttcttg
    10681 tggccgaaca tgtcggtctc gtggtcgcag aagaccatgt ccccgcccgc cggcgtcgcc
    10741 ccttcggcga cgatgacgat ggcgtagcgg ctggcgttct cggcccggtc ctgggacagc
    10801 agctccgcga ggcgctccat gtcgaacggg tgctcgggga tcaggcaccg gtcggccgcc
    10861 gcggccaggg tgggcagaag ggcggtgaag ccggcgtaac ggccgaagac ctcgatgacc
    10921 aggaagcgct cgtgcgagcc ggccgaggtc cgcagggcgt tggacaaggc gatcgtccgg
    10981 gtgacgcagg tcgaaaatcc gatgcaatag tccgtcccgg gaacgtcgtt gtccatcgtc
    11041 ttgggcacgg ccacgacctt gacgccctgc tggtggagat gaaccccgta gctcaaggtg
    11101 tcgtctccgc cgatcgggac cagataatcg atgcctaggt aatcgaggtt tttcaagata
    11161 tccggcgtca ggtcgttggt cttttccttg taggcctcgc ggagatgggc cggcacggcc
    11221 gcctgcggga catggctggg gcgggtccgg gatgtatgaa gaaatgtacc gccggtccgg
    11281 ccggctttcc ggacgtattc ctcggtcaag agacagtaat gggccgtgtt gtccgcttcc
    11341 ttgtcccgga ccatttccat caacccggcc cacccccgct tcaatccgat gaccttgtag
    11401 ccttcgctga gggcccggag ggtgaccgcc cggatggccg gattcagccc ggggacgtcg
    11461 cccccgccgg tcagaatccc gatgacgcct tttttggatt tggtgttggc catgatcctt
    11521 ctccttcctc aagcggccga gtggcggccc tcggtttcaa aattatacgg gaacgccggg
    11581 gaaaaatcca tgtttcggga cctcggacgc cgccggacgc cggttttccg ggcgattccc
    11641 cggtttgttt gacttcggat attcgtcctg gtatattcgc ggagatttcc gcgtttcctc
    11701 cccccgggca tgaaggccgt ctgtcgagcc gccgggaggg ggtccggccg ggagggcggg
    11761 ttcgggggga accgtccgcg gcccgggccg cggaggatca gccaggaggg attcatgctt
    11821 gaggccatgc aggaggcgtt gatcggcggc cggaaggccg acgtcgaggc tctcgtcgac
    11881 agggcgatcg ccgggggtct gcccacccgc cgtattttga acgaaggcct gatcgccgga
    11941 atggaaaagc tcggcgagct gttcaagaac aacgaagtct ttatccccga agtccttgtc
    12001 gccgcccggg ccatgaacgc cgccctcgtc aagctcgaac ccctgctgat caaggagggc
    12061 atcgagcccc ggggatgcgt ggtcatcggc acggtcaaag gggacctcca cgacatcggc
    12121 aagaatctcg tggccatgat gctcaaggga gcgggcttta aaatcatcga cttgggcgcg
    12181 gatgtggccg ctgataagtt cgtcgaggcc gcgaaatcca atgaagcgga cgtgatcgct
    12241 ctttcggctc tcctgacgac gaccatggtt caaatgaaga cggtcgtcgc cgccctggaa
    12301 gcggccggcc tgcatgtccc ggtgatcgtc ggcggggctc ccctgacggc cgattacgcc
    12361 tctcaaatcg gggctcgggg atacgccccg gacgcggcca gcgccgtgga cgtcgttcat
    12421 cggctggtcg gctgagaaac tttccttagg ggcgcgcttt ccgggcggcg cccgggagaa
    12481 cggacgccgt cagttcgttt cgggaacgga cggcagcttg aaccacaggc tgatcgccgc
    12541 gcccccctcg cggcggacgt cgatccatcc cctgagttgt tcggccagca gggcgatgat
    12601 ctgaaatccg agggattcaa cctttccggg cttgcatccc tccggcaggc cgattccgtc
    12661 gtcccggacc gtcagccgga tgatttcctt ttcgttttcc cgtagctcga cccggatcct
    12721 tccccgccgg ttgttgggga aggcatgctt caaggcattg aggatgagct cgttgagaat
    12781 gaggccgcag gggacggcgg tgttgatgtc gagataaacc ttatccaggt caagcttcag
    12841 ggcgatgcgg ccgtcctcct catgggaggc gaaaatcgtc gaggtcagcg acttgatgta
    12901 gtcggcgaaa tcgatccggg acaggtcctt cgtcatgtag agcttctcgt ggaccagggc
    12961 catggacttg atccgcccct ggctttccag gaaggcctcg cggacctcgg cctcggtgaa
    13021 cctggcggac tggagacgga gaaggctcga aatgacctgc atgttgttct tgacccggtg
    13081 gtggatctcc cgcaggagga cttccttttc ccgaagggcg tccaggatcc gcttctcggc
    13141 ccgttttctc tcccggatgt cgcggatgat ggcttggtga taccgggcgc cgccgatatc
    13201 gatcaagcgg aggctgactt cgacgggaaa ggcgatgccg tccttgtcgc ggtggagggt
    13261 ctcgaaaatc aggctgccgg tcttttccgc ctcctcgaga tggcggggga agtcggcccg
    13321 ggcttcccat ggccgcagat ccggaaccct gaacccgatc aattccgccc ggctgaatcc
    13381 gtaaaccgcc agggctcggt cgttggcctc gatcacccgg tgatcccggt cggctaggat
    13441 gacgatgtca ttggcgtact tgtggaggta ttcgaagtgc tggaccaggg ccaagcggtc
    13501 gtgctcgatc tcatactgtt tgcggaaata agccgcctcg cgccgcttga tccaaaacag
    13561 gagcatgacc ccggtcccga aacagaggat gatgatcatg gaaacgaaag acgacattct
    13621 ggctaaaagg ggctccagga tttcggacaa gtcctcctgg gcgaccaccc gccaggatgt
    13681 tccggggacg gctttgagag ccgagaggac gcggacgccc cggtaatccg ttccctcgaa
    13741 cgcgccgttc ttctcccttt cggccatgga ggccggaagg tcggcctcgg ccgcggggag
    13801 gcgcagcttg aggccggagt ccgggcggaa ccgcggatct tgcaggaaaa ggatgtcgtc
    13861 tccctcgcgg cggacaagca atgtctcgac gctccggccg ggcgcgcccc ggctccgcat
    13921 cgttggaatc agaaaatcgg ccgggtcgat ccggaacagg aagatgccgt cgcaggagac
    13981 tttcccgtcc ggcccgcggc cggagaccgg cgcgtacaga tcgaggtgcg ccttcaaccc
    14041 gatttcggag gaggtgtgaa agccggaaag atacggttcg cccgtggcga gcacgcggtc
    14101 gatggtttcc tcggcgttgg ttccgatgac gggcccgctg gagcccgaag ccattctcac
    14161 tcgtccgtcc gtgtcgagca ggacggcgtc catgtagcct ttcgccttga cgagggaatc
    14221 cagccaagcc ttgatccggg ggtcggccgt cgaaaccgcg ggatccgctt ccaggaaagg
    14281 gatgatcatc gcctgattga acgggttggc ggagatgatg gccgcgtccc ggagccgctc
    14341 ccggcgccag ccgtccagga gggagacctt gagatttcca acggcgttca ggttggccgc
    14401 ggccgtcgcc aggatctgtt ccttggattg ggaataataa atccagcccg cggccgacgc
    14461 caggaggaga agaacgatga acaagaggag ggggacaaga accggagagc gcggcatctt
    14521 cactctccca ttgtaataat tccaaggcgg ggctgcaata tcgacggcgg gaccgccgcc
    14581 gagaattccc tgcagcgatt cggccgcctt ggttcccggg cggcgcggga aggcccggcc
    14641 ggtttccccg ggacgggctc agaaccggat cgagaccagg atggcgccgg cgaaggccag
    14701 ggcgacggcc gccgctttca ggggggtgat tttttccttc aagacgacgg cggccagcag
    14761 gaagacgaag atggtgttga gctgattgag ggcggaggcg actgaggcca gcgtatattt
    14821 catcccggcc atccagatga ggacggagaa gtaagccgaa agcacggagg cgggaatcaa
    14881 aagcttgtag ttggcccccg cccgcagcgg ccgcaaaagc tcccggcggc ggggatgcag
    14941 agcccataag accagcccgg cggccgcccc tccggtcatc cggatcagag tcgcccagac
    15001 caccgaaatc cctccgagga agggtttgac gaggacgatg ccgcccgcgg tgagcagcat
    15061 cgagacgatc ccgatcccga cgccggacag gagggcgcga cgggaaagcc gctcgtcggt
    15121 ttttttgcgg gtgatcagga tcacggcccc gaggatcaag cccaccccgg cgatctggag
    15181 gcggttcatc cgctcgccga gaaagaggaa cgacaggccg atgacgaagg ggctgtaagc
    15241 gcagtcgacg atcgccgtca gctcggctcc gagccggacc agggccgtga aaaacagcca
    15301 gtccgagagg gcgatgccca ggatgccgct gagaacgaag agggcgaaga tccggggcgg
    15361 gaggtccggc gccccgccga tgaagaggac cgtcccgccc aggagaacca gggccagaac
    15421 gttcttgaac aagttcatca ccaggggatg gatcgtgtcg ccggcgatcc ggaacaggat
    15481 cacggagacg gcccagacga cggccgaggc gagagaaagg atctctccga tgtgcggcgt
    15541 ctgcatcaag ctgtcgaaca tgacggtcgg aagctcccac ttatagcaga gttcccgtca
    15601 aatatccagg agcctgctcg cgtccccgaa gccggtttga tgtcgggatg aggccggata
    15661 acgcctcgaa tccttcttct gaaattctca tcggccggaa aagggaaggt cggcgtcttc
    15721 gtggagaaac cccgaaataa aggccgcttg gcggcccatg gtttggagaa ggcgatttcg
    15781 gccgtttcga ttcgtgtata atgaaggctc aataagcgcg ccgagggcaa atcgaccatg
    15841 cgggacatcc gggacctgag ctttgacgag ctgcgggccg tcctggccga ggacggcgag
    15901 ccggcttacc gggccgagca ggtgttcgcc tggctctacc ggagaggggc gtcctcgttc
    15961 gccgaattca ccgacctgcc gcggcctctc cgcgaccggc tggccgcccg gttttccctc
    16021 cggccgctgg aggccgtgga aatccggtgc tcggccgacg ggacgacgaa aaccttgttc
    16081 cggctttccg acggatggaa tatcgaaacg gtgttcatcc cctccgggac ccggcggacg
    16141 atctgccttt cgacccaggc gggatgcaag ttcagctgcg cgttttgcgc cagcgggaag
    16201 ggcggcttcc accgccactt gacgccgggc gagattctcg gtcaagtcct gcatttccgg
    16261 agcggcccgg acgccggctt gacgaatctc gtattcatgg ggatgggcga gccgctggac
    16321 aacctggaca acgtcgagcg ggtcctccgc gtcctcaacg atccccgcgg attcggattc
    16381 gccgcccggc gcatgaccgt ctccacctgc gggctcgttc cggggatcga gcgcttcgcc
    16441 cgcatcgacc tccagatcaa cctttcgatt tccctccatg cggccgacga cgagacgcgg
    16501 aaccgcctga tgccggtcaa ccggcgatat cccctggccg agctgatttc cgcctgcgag
    16561 gattatctgg cggccggcgg gcggaaaatc accttcgaat acgtcctgct caagggcgtc
    16621 aatgaccggc cggccgacgc cgagcggctg gcccggctcg cccgccgtct cggggcgaag
    16681 gtcaacctga tcgccttcag cccggtcgag gggcttccgt tcgagcggcc ggatcccgag
    16741 gcggcctcgg ccttcgccgg gcggctcagg aacaaaggga ccgcggtgac cgtccggagc
    16801 tccaagggaa gcgacattca agcggcttgc gggcagttgg ccggctcgcc ggccgccggc
    16861 tgattggtca tttccggacg aggacggcca acgtttcgat gtggggggtc tggggaaaga
    16921 agtcgaacag ccggaccgag gccggggcgt aagccgcccc gagaaggccc aggtcgcgga
    16981 taagagtggc cggattgcag gataaataga tgatttttcc ggccgggcgt tcaatcaggg
    17041 cccggacgac cgccgggccc aatcctttcc tcggaggatc gaggatgacg gtgtccagtc
    17101 cgcgcccggc cagatccttc atccaggctt cggccgatcc gtccaggact tcgacgttgc
    17161 cgaggccgtt tcggacgata ttggcctcga gccggcggac gttgtcggga aacgactcga
    17221 tgacgaagac ccgggcggcg gagcgggcca gcgccaagcc gaacgtcccg acgccgctgt
    17281 aaacgtcggc gacgatttcc cggccccgga ggccggccgc ttccgagacg gcctccagaa
    17341 cgagagggag gatcgccccg ttgacttgaa agaaggatcc ggccccgatt tcgaaggtca
    17401 aaccggccgc tcgctcgagg agaatctccc ggccgtcttc caggaaggga agggatcggc
    17461 cgcttcttcc cggcgcccag gaaacgaggc cggagacggg aaaacgcgcg gcgagggcgt
    17521 ctcgaaccgc ggccgcggcc ttctcgggcc ggcgtttccg ccagaacaag ttgaggagga
    17581 gggtcccctc ggtccggctt tcccgggctt cgacgtcttc gagatccgtg aggccgccgt
    17641 cccggacggc ctccaggacg gtccgggccg cggcgcagga ggcctccgag gcgaggtggc
    17701 agcggccgtc cgtcgggacg aacgccgccc gggaacccgg ggaatgataa gccaggaaca
    17761 cgtccgggcc ggatcctctc acggaaaacc ggattttatt ccggtagttc cattccaggg
    17821 gggaagcggt gacctccggc tcgaaaaacg ggccggtccc aagacgccgg aacatctccc
    17881 ggagctggga cttcttcaga tcgagctgct cggagtaggg caagacttgg taggatccgc
    17941 aggccgaaaa gtggccgcag cgggcgggga tccgccgcgg agagggggaa aggacggccg
    18001 tggtccgggc ctcgacgaag gaggcgcgtt ccttgaggat ctcgatttcg acggtctccc
    18061 ccggcaatcc ctcgtcggtg aaatacaccc ggccttcatg ccgggccagg cgccggccgg
    18121 gatagacgat tttctctatc gacagccgga ttctcgttcc cttctcgaag gtcatgggag
    18181 ggcgggaagg cggccgccgc ggaaagcgga ggggaatcca tggccgggac ggagacggat
    18241 gtcgggcacg ccggctccta gaaatggaga aagatgccgg cgaacgtata aacggcgaat
    18301 ccggtcatga caatggccaa gatcaggaag atgcgctgga aggtccgggg tttgaattgg
    18361 tgatggtgat gcgagacgta ataggaaacc accgtgtacc aaataaagcc gcccagcccg
    18421 cacgaagcgg cgaataaata gggggcggcg gcgtaattga gaatccagcc gtgcccggcg
    18481 gcccaaccgg ccgcccagat ccagaagaaa tacagcgacg ggttggagac gtagagcagg
    18541 atgacgccga ggatcggccg aggcgtaaag gaggtaggga gcttttcctg cttggggatg
    18601 accggcttct tcgacgtcag gtagagccgc cagccgatga tcaacatgat gatgccggcc
    18661 gcgattttca gcgggatttg gaaacgagtg atgttgacgg tgatgaaggc gatcccgagc
    18721 agggagatca agcaataaag gaaatcgaga acggcggcgg tcgccccggc cacgaagccg
    18781 tggaagaagt tccgtttcat cgactgggaa atgacgaagg cattcaccgg cccgaggggg
    18841 atggcggcga cgaaaccgag aatgagaccg accagcagga acatgttgca gggaaaaata
    18901 ccagaagaaa cggccgggtg tcaacgagga ggggggggaa cgaacgaact tgactttatc
    18961 taaacgatag gttatagtgt actcactcct gttgtgcagg ctggacagga acccctcctt
    19021 ttaccccttt agcttaattc cctgaaaaac agcctgcttc acccctcaaa gaaaaaggcg
    19081 ggagggaaag ccctcccgcc ttttcatttt aaatccgatg atcgtttctt ctctactcct
    19141 gggccgctcc gccgaaatgg taggacgcgc cgatggagaa gacgaaggtc gtctgctggt
    19201 aaagctcccg gaaggaagcc ccgagcgcgg gataaggaag gttcttcgag taatccttgt
    19261 aattgacgat cagggcgccc aggtcgacgt tgaagttcgg gctcagccgg atccggccgc
    19321 cgaatccgaa cgtgtcggcg ttcagatcat agctcatgtc cgactggtag tcgtcgacaa
    19381 gcccgtaccg ggacagctgg tagcccgcgc tgaccatgat caggtcggag atgtcatact
    19441 cgaggccgaa ggaaaggtcg atggaattct tgtcgagcaa gtcttcccgc ccgtcccaat
    19501 cggcgttctt gtcgaagtaa taggagaagg acagcgaggc ccgaaaatcg ggcgaaatgc
    19561 cgtaatccgc cccgagggcg aggaaacccg gaatgtcgcc gtgcgtcttc tggccgtccg
    19621 ggaacaatcc cgtcccgtcg accgtcgttt tgttggtcag ctcgatcttg gtgttgaatt
    19681 catagcgggc gctgaggttg agctccggga tggggctgta gtggaggctg aggatcgggg
    19741 tgaagcccga acccgtctgc ttggcgtcga cggccttgtc ggcgacca
//
LOCUS       DAOOXH010000042        19721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_2634_length_19721_cov_7.300519, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000042 DAOOXH010000000
VERSION     DAOOXH010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 19721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19721
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2634_length_19721_cov_7.300519"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(280..354)
                     /locus_tag="PKV64_06425"
     tRNA            complement(280..354)
                     /locus_tag="PKV64_06425"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(317..319),aa:Arg,seq:cct)
     gene            complement(470..2104)
                     /locus_tag="PKV64_06430"
     CDS             complement(470..2104)
                     /locus_tag="PKV64_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPN16752.1"
                     /translation="MTNIASVSQTVYQDLAVAFGVLAAIVILSALARAAFSRFPGAGK
                     KGRWILASRLAFPLIALAVLLAVRAVLSLSQAMESYLNAAFFFFVIFLLVRLFDAAIL
                     AWYVDRRKAYPLPKVLRGLIIGLILLVALFSILKNMLQMDISSLLAGSAILTAVIGLA
                     LQGVLSNILSGMSLHFTRAFKQGDWISLGTHEGVVMDTNWRETRLLDRQSNVLILPNN
                     VVAGEKIVNYSEPDRRTALFLNLKVSPVAPAAGILEALTEAAVECPFVAAEPKPLAYL
                     TSFDETGISYALKFFVEEYGLKPVIITDVGRLAWYKLRRRGFEVAVSWPDRLGDMKEA
                     IQAAGPPRPAGEGEIETLTAREREARRTAEILLASSFLRYPDGEKAGQSMVPESELRA
                     LAGRIRRSTFTKGEVLARQGDRGASCFVVARGRIRGEIVYEENGKRFVKEFETVPGGL
                     FGEMTLFSGMPRTATGIIAEESELLEIEARDFRSLLVRNPEAAEAIADLVSARNAQNK
                     EFLLKIKELSARDVEEGSDRRTVLAYLKRFIHGLLG"
     gene            complement(2109..2933)
                     /locus_tag="PKV64_06435"
     CDS             complement(2109..2933)
                     /locus_tag="PKV64_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPN16753.1"
                     /translation="MRIEFFGVRGTAPTAPRRGSVYGGHTPCALVRTSAGTDFIVDVG
                     TGLVSLAKSPAFSRRTSLRILHILLTHFHFDHVSGLPFFPEIFRAGADLRFYSSGPAA
                     ALRRRLEEFMTGPYFPVEFKATPSRKSFGRVGRRPASVGGAKVSSHPLHHPQGCAAFR
                     IEENGRSVVFATDTEHGPGGADEGLAAFARGTDALIYDATFTPAEYAAGRKGWGHSTW
                     AAGVELARAAGAARLVLSHFNPDHSDAKLGRLEDAARRRFPRSLFAREGLILRLER"
     gene            complement(2957..5065)
                     /locus_tag="PKV64_06440"
     CDS             complement(2957..5065)
                     /locus_tag="PKV64_06440"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPN16754.1"
                     /translation="MLKKRGARGLAVGAAAFALILGLDAAGVFRRLEWKAWDARMSVF
                     ARPAKASPDIVLILVDQYSLDFYQEQGITWPWPRELYGAIIRHLAAGGAAAIFFDIAM
                     TEGSRFGVEDDRAFAAAAAAAGNVFVPVFLSLDEGSSGPGIDPAAGELFRAKAEAASG
                     PSGPPGSAAEVFPSVTLPLTEILGAARGAVNVRVNPDADSIYRRIPLFFSYRDDFRVP
                     SVPWALASAVRPGLDPRTVPVDREGRIVIRFYGPAGTYRAYPAAAVINAYAQTLEGTN
                     PQIPPEEFAGKTVLVGLSAVGLFDIKAGPLSGVMPGVEIQAAALDTLLHGKGFRFPSR
                     GAAWAYLAFLALAAGLVLSLLRKTAAQAAASAGFLLLPAFGAAAAFQAGIWLDFSAPA
                     AAVLLSLVASAVLNYGVEGRERRFLKGVFHHYLSPAVIDRILEDPGRLRLGGEEREIT
                     SFFSDVAGFTSISEALEPAVLVSFLNEFLTGMTDLILDAGGTLDKYEGDAIIAFWNAP
                     LDDPDHALRAAQTALACQARLAAAAPEWEKKYGRRIRMRIGLNTGPAIVGNMGSSRRF
                     DYTAIGDTVNLASRLESAGKQYGADLLAGEATVKKAGEELVFREVDIVRVVGKTKPVR
                     IYELIGEKGKISGETARRLELYGRALGLFRRRAFGEAEDVFSRIENDAPAVLYRDRCR
                     KLRASPPPDDWDFIYDLQVK"
     gene            complement(5114..6028)
                     /locus_tag="PKV64_06445"
     CDS             complement(5114..6028)
                     /locus_tag="PKV64_06445"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPN16755.1"
                     /translation="MNARSRAARALLLAVLLAAGAACSLIDQGTEILGKAEDAVAKGD
                     KAVKAYGKLRSAFGDFTEEEEYYIGRSVAALILGRYPALNDAALNLYVNKVGNAVALS
                     SERPEIFAGWHFQALDTDEVNAMAAPGGMIFITKGLLKRCPDEESLALILAHEVGHVA
                     ARHGLQSIRKANVTEAFTSIGLEAIKTYGPEQLARVTAAFEGVLGDIVGTLVERGYDR
                     KLEYEADESAVLFGCRTGYDPAGLARFLKTMVGDMSAVSGKGWFKTHPAPEQRIDRVQ
                     AEIDRLQPVPAPAAVRTQRFKSAVSAVR"
     gene            complement(6025..6501)
                     /locus_tag="PKV64_06450"
     CDS             complement(6025..6501)
                     /locus_tag="PKV64_06450"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPN16756.1"
                     /translation="MKHPRKAVLAAGVLAVFCLAAAADVLVVKIQSTQIRAQAQFFAP
                     VVAGLKAGDALTQIGVSGAWIQVRTAAGLSGWIHQSAVEVPRYALAAAAGGTRTQATA
                     SEAALAGKGFNKQVEENFRAGHAEADYASVDRMLQNKMLWSQLKEFLDQGKLGGGR"
     gene            complement(6529..8742)
                     /locus_tag="PKV64_06455"
     CDS             complement(6529..8742)
                     /locus_tag="PKV64_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HPN16757.1"
                     /translation="MSETLEGTIEHILFFNPDNGYSVLRFVSDGGERLTVVGEFPPLS
                     AGERLRVAGTWEMSPKFGRQFKAETFIPSLPAGRTGIEKFLACGLIKGIGPVLAKRIV
                     DAFGGETLEVLEKTPERMREVAGIGEVKYKEIRKSWAEHEDIRGLIIFLQDHGVSTNL
                     ATKIYRHYGPKAYDVLRTNPYRLAQDIWGIGFKTSDAIALKLGLDPASPERIKAFLLY
                     LLDKDNEQGHVFSFAAGLARSCREELGTEEEAFGRALADLIRDRAVVVEELPGDRAVY
                     RPYYYDAQEDVVAAIRNLAGAPSFAPPFDIDAAAAEIELSFGITFSELQRRAVRESVQ
                     RKILVITGGPGTGKTTIIRAVAALFAKWGKTVLLAAPTGRAAKRLSEATGREAKTIHR
                     TLEYNPKTGRFRRDPGRPLQGDAIIIDEFSMVDLSLMHGLIRAIPSSMRLIIVGDKDQ
                     LPALGPGTLLRDLIECGRVAVVRLDEIFRQERDSLIVVNAHRINQGQGILHPKKGDPE
                     SDFYFIRADEDAKAFAAIMQLCGYRIPRRFHLSPLSPQIQVISPMYKGFCGVDRLNAE
                     MQARLNKSGEVLRLGSREIRARDKVMQVRNNYEKDVFNGDVGTVVHIDPVRYGLVVEF
                     DGRPVFYDRDELNDLALAYAVSVHKAQGSEYQAVVLPLLTQHFIMLQRNLFYTALTRA
                     KKLSVVVGSYKALYIAIKNDKPVQRNSRIKEKLLAALETSGSSGGEAFDLTGGLF"
     gene            complement(8951..12148)
                     /locus_tag="PKV64_06460"
     CDS             complement(8951..12148)
                     /locus_tag="PKV64_06460"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HPN16758.1"
                     /translation="MYSASSLFFFTALALTFILAGLLAGYLRRRRDLPGARAFFWLAV
                     AQSLLAFAEILSLFAGSAGQALFWFKIRFVFSALMPVFWIWFALDYSGRKKWIGRRLA
                     AGLFLIPVITQVMVWTNDRHHLFVKREVGFVLKGPFWIADTGVRTAGVWFLVHSFYGL
                     ILLFAGMALVTFALLKKRSRSVGPVFFINAAAFVAVANAAVSAFNLFPQAEFNPYTPG
                     MGVSFLLVAIAVFPFRLFHRAPAGETGEEDVSADRSLAAFLFILIVLASGITAAGYVS
                     FRTFQSNLRVQAENQLTIISRLKADELRDWRRERMADASLLQADSYFAELVERFLSRP
                     SDFEARDEIQSRLLRYQAYRQYLRAYVVDMEGTARLTVPLPPRAHEAFNKSPIAVAEA
                     IAANQAVLHDFHREGEESIHLSLYVPLVLYRPGSASRPLGAVVFWIDPAAHLYPYVLN
                     WPGPSASAETLLVRRDGDTVLYVSDPKFRPGAALNLRLPLTRTEIPAVQAVLGREGIF
                     EGKDDHGTAVLAFLRAVPESPWYLTVRMDLAEINAPARERLWQMILMLAALITALGAA
                     LRTIWRRQRLQFFRSRAEIGQKLRDSLERFELANKATFDIIWDWNLQTGTYWLNENFH
                     ELYGYSPAETEPNITSWIRRIHPDDRDRVVAGIRAVIDGGEEFWSDQYRFLCKDGRSL
                     EIFDRGFVTRDGAGKPLRMIGAMQDISEQRKAESALSHFHDLMRYIIEHNRSAVAVHD
                     RNLRFLYVSQRYLDDYRVKEKDVIGKRPDDVFSDFPGKWSEIYQKGLAGLISSAEDDP
                     YPRADGTTDWTRWECRPWYEADGAIGGIIVYTEIVNERKKADEAIRASLREKEVLLKE
                     IHHRVKNNMQVISSLLSLQAAQFNEDRFRDAMRETQRRIRSMALLHEKLYKSRDLSRI
                     DFVEYARSLATHLLQSHAIDPTRITLRFEMEPVFFDLTTANPLGLILNEFLSNAMKHA
                     FPGNRKGEIFVSLRRRGDEYLLHVRDDGIGLPEAFDPRGKESFGIQIIDALVHQLNGR
                     LRVEGEGAGATFELAFRELVYGPRLDPALQSTDKS"
     gene            12375..14030
                     /locus_tag="PKV64_06465"
     CDS             12375..14030
                     /locus_tag="PKV64_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPN16759.1"
                     /translation="MRVLRLLANGLICGIYFCFLLALLVLDLNINRPAAAFDFVRLAV
                     FLFPTYGLLSALASILTASLYRFFVTRPSERNFLSPRFLTLGFSLLTLIFLVVFRENT
                     IHFASFFTPAVRRSVQMQAAVLFLAASAGLVLLFRYFHRRRRAAFLGASLAAAVVALA
                     AACLLRIAYPAADTPFRLSSMEAKPIARRVTILNLEGLSFDFIYPLVNRRLLPNFAYL
                     MENGSWGHLAGFTPSDTAVLRRTLKTGKLPGKHRRLSDVRYTIPGLPGHLEAVPRFIL
                     FRQLKQLGLLKIEPYDPPPAVKDIEMIYGEEQAVVVRSEERTALAPETLPEAPAADKL
                     FLSLYKDLQDERAPICRRLEAALIRDRITEERAFRSKAELPPQLFLLTLDGLDDVQTY
                     FYKYSVPAEFGEIRPDEIQKFGTVIERYYQYYDQIISKYTAAMKDDEILIVCSPFGVA
                     PLPFWKRLVEWVLGNADVSAYHEQAPDGAVFFYGSGVSRGRSADGLRLADLAPTLLYY
                     AGLPVAKDMDGFARTTIFTREFKERNPLIAISSYEDVTIRKRR"
     gene            complement(14062..14775)
                     /gene="lepB"
                     /locus_tag="PKV64_06470"
     CDS             complement(14062..14775)
                     /gene="lepB"
                     /locus_tag="PKV64_06470"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPN16760.1"
                     /translation="MTKEKADGKKKRTKSVFREYFELIAETAVFVFFVMTFVVQAFQI
                     PTGSMEPTLLVGDFLLVNKFVYDNGSPPLDRLLLPRRDIKRNDIIVFKYPNELSKDYV
                     KRVIGLPGEKFEVRDKQVYINGQPLREPFKVHSDAQVFAKNGYYHYEDTIRDNFGPVT
                     IPPGHLFAMGDNRDQSSDSRYWGFVPLSYVKGRPWIIYFSYAAERDAYLKTSVRDRLK
                     KLVRFIPKARWNRMLKIIR"
     gene            15104..16228
                     /gene="tgt"
                     /locus_tag="PKV64_06475"
     CDS             15104..16228
                     /gene="tgt"
                     /locus_tag="PKV64_06475"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPN16761.1"
                     /translation="MKKRFIVETTAAGSSARAGIIRTPHGDIPTPAFAPVASQGAVKG
                     LTHDQVTALGAHLILCNSYHLFLRPGAEAVRAMGGLHAFISWPKPILTDSGGFQIYSL
                     EPLAKVRRDGVAFASHLDGSKLFLTPEDVVDIQALLGSDIMMVLDHFVPFASPESKVR
                     EAVETTIRWAGRAREAFRKRESRAHLWAIAQGGVIPELRRRCAEELRDMDFDGYAVGG
                     LGIGEAKAQLHKTLEQSDALLPKDKPRYLMGMGYPADILEAVDRGVDLFDCVLPTRNA
                     RNGSLFTRRGVVTIKNRKYADDLRPIDEDCGCPTCRRYSRAYLRHLYERKEITSAVLN
                     TTHNLHFYLDFFKEMRQSIQSHSFQQFKLNLMNTLKEVNR"
     gene            16225..16584
                     /gene="yajC"
                     /locus_tag="PKV64_06480"
     CDS             16225..16584
                     /gene="yajC"
                     /locus_tag="PKV64_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPN16762.1"
                     /translation="MIFSALFAAQQGQVPAGQAAPQGNMLTALIPFVLVFGIFYLLII
                     LPQRKKQKKHQERIEALKPGDKVVTTGGIFGTVMGVLKDRIELKIAANVKIEITKGAI
                     GVILSQGEASPAEKPES"
     gene            16678..18213
                     /gene="secD"
                     /locus_tag="PKV64_06485"
     CDS             16678..18213
                     /gene="secD"
                     /locus_tag="PKV64_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPN16763.1"
                     /translation="MKKGLRWKVLLTLGALGLSLFLALPLKEKIKLGLDLKGGIHLVL
                     QVMTDDALNGETDQEIIRFTDLFKKNSIRFTTAAKEKPGRFVFTGLDVDQQIKARDLV
                     AQYTRDWDASFMADRVTFTLKASAVTFLKDQAVVQTVETIRNRIDQFGVSEPLIQRQG
                     ENQIVVELPGVDDPERVKDLIKVTAVLEWKLVKAGPAEDEATLLQSFGGTVPDDAEIV
                     RGDPARGHPGVYLVEKVATVSGKDLRMVRRGTDEWGNPAVSFTLSSDGGRRFEQVTGA
                     NIGRALAIILDDRVQSAPNINARIGDSGIIQGQFTIQQADDLVVVLKAGALPAGIKYL
                     EERMIGPALGSDSIRQGMAAGLVAILLVMTFMVVYYRLSGVNAVIAMILNVVILFGVL
                     AYFKANLTLPGIAGLILAIGMSVDANVLIFERIKEERALGKNVPGAISLGFSRAFTAI
                     FDSNLTTIISAVFLFQFGTGPIKGYAVTLIISLVANMFTAVFVSRLIFDLVIPKTAVK
                     LSI"
     gene            18215..19378
                     /gene="secF"
                     /locus_tag="PKV64_06490"
     CDS             18215..19378
                     /gene="secF"
                     /locus_tag="PKV64_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPN16764.1"
                     /translation="MEIFKKTPQIRFLKYRGIAFIVTIVIIAAGMLNIFAGRGLKLGV
                     DFGEGTLIRVMFKNPTDVGDIRDVLADIGLGSSMIQELGRGGREFQIRAVEVKSTVEE
                     SRLESHEVLGNRIIEALKGTDGQNETAQGLADLNMIDQKSLAALLEPSFLERSSGLAE
                     QILSVRKNDRNQGIFKDYADLSADGVPAEVVTSLKGKTYLGKLNILSRESVGPQVGKD
                     LRKKALMATLWSLVGMLIYIAVRFKMANGVAAIFTLTQDVLITISLYSFSGREINLPI
                     VAALLTIVGFSINDTIVIFDRIRENQKANRKMPVEDVMNLSLNQMLGRTFITSGTVLM
                     TVLALFLFGGPVINDFAFCMLIGTVEGIYSTIYLACPVILAWQKWFPSKRSRR"
     gene            19486..>19721
                     /locus_tag="PKV64_06495"
     CDS             19486..>19721
                     /locus_tag="PKV64_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16765.1"
                     /translation="MAEQTKPTSPVKERTADIFDESFFVAGKAAGRKALAFPVAVVLH
                     LILVVALVVLPLLSVSNLPQIEITSAFLAPPPPPP"
BASE COUNT         3806 a         6308 c         6071 g         3536 t
ORIGIN      
        1 ttacgatctg ctacctggtc gaatatacgg aggactggag cggggagcga agctatatcc
       61 gggaggataa gatcatcgga tccttggaaa agttccacga gctgacgagt caaatcgcat
      121 gctgaagaga gttacgatgg cccccatttt gcaaacatcc gttgacacgg cccaagagag
      181 aggaagtgtg cttaaaaaga gacataaaaa gacataaatc agctaaccca aaaaatagaa
      241 acatccttct atctaacaga taataatagc aatatgaaat gcgcctggcc cggctcgaac
      301 gggcgaccta gggattagga atcccttgct ctatccatac tgagctacag gcgcacaaat
      361 tgaaggcgtt ttcatcttaa tccaagcggg gcggaaattc cacttccttc cgcgcctcct
      421 tccgcgccgc cctcaaacgc cgaagcggga agatccgcga caatccctct taacccagca
      481 gtccgtgaat gaaccgtttg agataggcca gaacggttcg gcgatccgag ccctcctcga
      541 cgtcgcgggc tgagagctcc ttgatcttca aaagaaattc cttgttctgg gcgttccggg
      601 cgctgacgag atcggcgatc gcctcggccg cctcgggatt ccggacgagc agcgaccgga
      661 agtcccgggc ttcgatctcc aggagctcgg attcctcggc gatgatcccg gtggccgtcc
      721 ggggcatccc cgaaaacaaa gtcatctcgc cgaacaggcc gcccgggacg gtttcgaatt
      781 ccttaacgaa tcgcttgccg ttctcctcgt agacgatctc tcccctgatc cggccgcggg
      841 ccacgacaaa acagctcgct cccctgtccc cctggcgggc aaggacttcg cctttcgtga
      901 aagtcgagcg gcggattcgg ccggccagcg cccggagctc ggattccgga accatcgatt
      961 gtccggcttt ttccccgtcc ggataccgga ggaaggagga ggccaggaga atctcggccg
     1021 tgcgccgggc ttcccgttcc cgggcggtga gcgtctcgat ttccccctcc cccgccggac
     1081 gcggcggccc cgcggcctgg atcgcctcct tcatgtcccc cagccggtcc ggccaggaaa
     1141 cggcgacttc aaatccgcgg cggcgcagct tgtaccaggc caggcggccg acgtcggtga
     1201 tgatcaccgg cttgaggccg tattcctcga cgaagaattt aagggcgtag gaaatccccg
     1261 tttcgtcgaa gctcgtcaag tacgccaggg gcttgggctc ggcggcgacg aaggggcatt
     1321 cgaccgccgc ctccgtcagc gcctccagaa tcccggcggc cggggcgacg gggctgacct
     1381 tcaaattcag gaaaagagcc gtccgccggt cgggctcgga ataattgacg attttttctc
     1441 cggcgacgac gttgttgggc aggatgagga cgttggactg gcggtcgagc agccgcgtct
     1501 cccgccagtt cgtgtccatg accacgccct cgtgcgttcc gaggctgatc caatcgccct
     1561 gcttgaacgc ccgggtgaaa tgcagcgaca tgccggacag gatgttgctg aggacgccct
     1621 ggagggccag gccgatgacg gcggtcagga tcgccgatcc ggccaaaagg ctggagatgt
     1681 ccatctggag catgtttttc aggatggaaa agagggcgac caggagaatg aggccgatga
     1741 tcagaccccg gagcaccttc ggcagcggat aagccttccg ccggtcgacg taccaggcca
     1801 ggatggccgc gtcgaaaagc cggaccagaa gaaagataac gaagaaaaag aaggccgcgt
     1861 tcagatagga ttccatcgcc tgggacaggg aaagaaccgc ccggacggcc aacaggacgg
     1921 ccagggcgat caggggaaag gccagccggg aagccaggat ccaccgccct tttttccccg
     1981 cgccggggaa gcggctgaag gccgcccggg ccaaggccga aaggataacg atcgcggcca
     2041 ggacgccgaa ggcgacggcc aggtcctggt agacggtctg ggaaacggag gcgatgttgg
     2101 tcatggcctt atctctccaa ccgaagaatc aagccctccc gggcgaacag ggagcgggga
     2161 aacctccggc gggcggcgtc ttccagccgg ccgagcttcg cgtctgaatg atcgggattg
     2221 aaatgcgaaa ggacgagcct tgccgccccg gccgcccggg ccagctcgac tccggcggcc
     2281 caggtgctgt ggccccatcc tttccggccg gccgcgtact cggccggggt gaaggtcgcg
     2341 tcatagatca gcgcgtcggt cccgcgggcg aaggcggcca acccttcgtc ggcgcctccg
     2401 ggaccgtgtt ccgtgtcggt ggcgaagacg acggaccggc cgttttcctc gattcggaag
     2461 gcggcgcatc cttggggatg atggaggggg tgggaggaaa ccttcgcccc gccgacggat
     2521 gccgggcgcc ggccgacgcg gccgaaggat tttcgggaag gcgtggcctt gaattccacg
     2581 ggaaaatagg gcccggtcat gaattcctcc aagcggcggc ggagggcggc ggccgggccg
     2641 gacgagtaaa accgcaggtc ggcgcccgcc cggaaaatct ccgggaagaa cggaagtccg
     2701 ctgacatgat cgaaatgaaa atgggtcaaa aggatgtgga gaatccggag cgatgtcctc
     2761 cggctgaagg ccggggactt cgccaaggaa acgaggccgg tgccgacgtc gacgatgaag
     2821 tccgttccgg cggaggtccg aacgagggcg cagggcgtgt ggccgccgta aacggagccc
     2881 cggcgcggag ccgtcggcgc cgttccccgc actccgaaga attcgatgcg catcggtcct
     2941 ctctttcttc ccggcctcac ttgacctgga gatcgtaaat aaaatcccag tcgtccggag
     3001 ggggcgacgc ccgcagcttc cggcagcgat cccggtaaag gacggccggg gcgtcgtttt
     3061 cgatccggga aaagacgtct tcggcttcgc cgaaggcccg ccgccggaaa agcccgaggg
     3121 cgcggccgta aagctcaaga cggcgcgccg tctccccgga gatcttccct ttctccccga
     3181 tcaactcgta aatccggacg ggtttcgttt tgccgacgac ccgaacgata tccacctccc
     3241 ggaagaccag ctcctccccc gcttttttca ccgtcgcctc cccggctaaa agatcggcgc
     3301 cgtactgttt gccggcgctt tccaggcggg aggccaggtt gaccgtgtcc ccgatcgccg
     3361 tgtaatcaaa ccgccgcgaa gaccccatgt tcccgacgat cgccgggccg gtgttgaggc
     3421 cgatccgcat ccggatccgc cggccgtatt tcttctccca ttcgggggcc gccgcggcca
     3481 ggcgggcttg gcaggccagg gccgtttggg ccgcccggag agcgtgatcc ggatcgtcca
     3541 gcggggcgtt ccagaaggcg atgatcgcgt cgccttcgta cttgtcgagc gttccgcccg
     3601 cgtcgagaat caaatcggtc atcccggtca ggaattcgtt gaggaaggac accaggacgg
     3661 ccggctccag ggcttccgaa atcgacgtga agccggccac gtcggaaaaa aacgaggtga
     3721 tttcgcgctc ttctccgccg agccggaggc ggcccggatc ctccaggatc cggtcgatca
     3781 cggccgggct caagtaatgg tgaaaaacgc ccttaaggaa gcgccgctcc cggccctcga
     3841 cgccgtaatt caaaacggcc gaggcgacca ggctcaaaag gacggcggcg gccggggcgg
     3901 agaaatccag ccaaatcccg gcttgaaaag ccgccgccgc tccgaaggcc ggcaggagaa
     3961 gaaagccggc ggaggccgct gcctgagcgg ccgtcttccg gaggagggac aggaccagcc
     4021 cggcggccag agccaggaag gccaaatagg cccaagccgc cccgcgggaa ggaaaacgaa
     4081 atccttttcc gtgaaggagc gtgtccagcg cggccgcttg gatttcaacg cccggcatga
     4141 cgccggacaa cgggccggcc ttgatgtcga agaggccgac ggcgctcaag cccacgagca
     4201 ctgttttccc ggcgaattcc tccgggggaa tctgggggtt cgtcccttcc agggtttgag
     4261 cgtaagcgtt gatcacggcc gcggccgggt aagcccggta tgtcccggcc ggcccgtaaa
     4321 agcggatgac tatccgcccc tcccggtcga ccggcaccgt ccggggatcg agaccggggc
     4381 ggacggccga agccaaggcc cagggcaccg agggaacgcg gaaatcatcc cggtaagaaa
     4441 aaaagagggg aatccggcgg tagatcgaat cggcgtccgg attgacccgg acgttgaccg
     4501 cgccgcgggc cgcgcccagg atttccgtca agggcagggt gaccgacgga aacacctcgg
     4561 ccgcggaacc cggcggcccg ctcgggccgg aggcggcctc ggccttcgcc cggaagagct
     4621 ccccggcggc cggatcgatt cccggcccgc tcgagccctc atccaggctg agaaaaacgg
     4681 gcacgaaaac attgccggcc gcggccgccg cggccgcgaa cgcccgatcg tcctcgacgc
     4741 cgaaccggga gccctcggtc atggcgatgt cgaaaaaaat ggcggccgct ccgccggcgg
     4801 ccagatggcg gatgatcgcc ccgtagagct cccgcggcca gggccaggtg attccttgtt
     4861 cctggtaaaa atcgaggctg tactgatcga cgagaatcag gacgatgtcc ggcgacgctt
     4921 tcgccggccg ggcgaagacg ctcatccgcg cgtcccaggc tttccactca aggcgccgga
     4981 aaaccccggc cgcgtccaat cccaggatga gagcgaaggc cgccgccccg acggccagtc
     5041 cccgcgcccc ccgttttttc aacatgacga atccgccggc cggaaatccc ctcctccccc
     5101 tccggtccgc ccttcaccgg accgcggaga cggccgactt gaaccgctgc gtccggacgg
     5161 cggccggggc cggcacgggc tgcaggcggt cgatctcggc ctggacccgg tcgatcctct
     5221 gctccggcgc cgggtgggtt ttaaaccagc ccttgcccga gacggccgac atgtcgccga
     5281 ccatcgtctt gagaaaacgg gcgagccccg ccggatcgta accggtccgg cagccgaaaa
     5341 gaacggccga ttcgtcggcc tcgtattcga gcttccgatc gtatccgcgc tcgaccagcg
     5401 tcccgacgat atcgccgagg acgccttcaa aagcggccgt gacccgggcc agctgctccg
     5461 gaccgtaggt cttgatcgcc tccaagccga tggacgtgaa cgcctcggtc acattggctt
     5521 tccggatgga ctggagcccg tgccgggcgg cgacgtgtcc gacttcgtgg gccaggatca
     5581 aggccagcga ttcctcgtcg gggcagcgct tgagaagccc cttcgtgatg aagatcatcc
     5641 cgcccggggc ggccatggcg ttgacttcgt ccgtgtcgag cgcctggaag tgccagccgg
     5701 cgaagatctc cggccgttcc gaagacaggg ccacggcgtt gccgaccttg ttgacataga
     5761 gattgagggc ggcatcgttc aaggccggat agcggccgag aatgagggcc gcgaccgacc
     5821 ggccgatgta atactcctct tcctcggtga agtcgccgaa ggcggaccgg agcttgccat
     5881 aggctttcac cgccttgtcg cccttggcca cggcgtcctc ggctttaccc aagatctcgg
     5941 tcccctggtc gatcaagctg caggcggccc cggcggccag gagaacggcc aaaagaaggg
     6001 cccgcgcggc ccggctccgg gcgttcaccg gccgcctccg agctttcctt gatccagaaa
     6061 ttccttcaac tgggaccaca acattttatt ctggagcatc cggtcgacgg aggcgtaatc
     6121 ggcctcggcg tgtccggccc ggaaattctc ctcgacctgc ttattgaatc ccttgccggc
     6181 caaggccgct tcggaggccg tggcctgggt ccgggttccg ccggccgcgg ccgccagggc
     6241 gtaacgggga acctcgaccg cgctttgatg gatccagccg ctcagcccgg ccgccgtcct
     6301 cacctggatc caagcgccgg agacgccgat ttgagtcagc gcgtcgccgg ccttgaggcc
     6361 ggccacgacg ggggcgaaaa actgcgcctg ggcgcggatc tgcgtcgact ggatcttgac
     6421 gacgagaacg tcggcggccg cggccaggca gaagacggcc aggacgccgg ccgccaggac
     6481 cgcttttcga ggatgtttca tgaggtcctc cttccctttg ttttcatctt agaaaaggcc
     6541 gccggtcaag tcaaacgcct ctccgccgga ggagccggag gtctccaggg cggccaggag
     6601 cttctccttg atccggctgt tgcgctggac gggcttgtcg ttcttgatgg cgatgtaaag
     6661 cgccttgtag gagccgacga cgacgctcag cttcttggcc cgggtcaggg ccgtgtagaa
     6721 cagattccgc tgaagcatga tgaaatgctg ggtcaggagc ggcaggacga cggcttggta
     6781 ttcgctgccc tgggctttgt ggacggaaac ggcataggcc agagccaggt cgttgagctc
     6841 gtcccggtcg taaaacaccg gccgcccgtc gaactcgacg accaggccgt accggaccgg
     6901 atcgatatgg acgacggttc cgacatcgcc gttgaacacg tccttttcgt aattgttccg
     6961 gacctgcatg accttgtccc gcgcccggat ctcgcgggat ccgagccgga gaacttcgcc
     7021 ggacttgttg aggcgggcct gcatctcggc gttgagccgg tcgacgccgc aaaacccctt
     7081 gtacatcgga ctgatcacct ggatctgggg agagaggggg ctgagatgaa agcgccgggg
     7141 aatccggtac ccgcacagct gcatgatggc cgcgaaggcc ttggcgtcct cgtcggcccg
     7201 gatgaaataa aaatcggact cgggatctcc ctttttcgga tggaggatgc cctggccttg
     7261 gttgatccgg tgggcgttga cgacgatcag gctgtcccgc tcctggcgga agatctcatc
     7321 gaggcggacg accgccaccc ggccgcactc gatcaggtcc cgcaggaggg ttcccggtcc
     7381 aagggcgggc agctgatcct tgtcgccgac gatgatgagc cgcatggacg acgggatcgc
     7441 ccggatcaag ccgtgcatca gcgacaggtc gaccatcgag aactcgtcga tgatgatcgc
     7501 gtccccctgg aggggacggc cgggatcgcg gcggaagcgg ccggttttcg ggttgtactc
     7561 cagcgtccgg tggatggtct tggcctcccg gccggtcgcc tcggaaagcc gcttggccgc
     7621 ccggccggtg ggggcggcta aaaggacggt cttcccccat ttggcgaaca gggcggccac
     7681 ggctcggatg atcgtcgtct tgcccgtccc ggggccgccg gtgatgacca ggatcttccg
     7741 ctgaacgctc tcccggacgg cccggcgctg aagctccgaa aacgtgattc cgaacgagag
     7801 ctcgatctcg gccgcggccg cgtcgatgtc aaacggcggg gcgaaagacg gggccccggc
     7861 caggttccgg atcgccgcga cgacgtcctc ctgggcgtca taataataag gacggtaaac
     7921 cgcccggtcc ccgggaagtt cctcgacgac gaccgcccgg tcccggatca aatccgcgag
     7981 cgcccggccg aacgcctcct cttccgtccc caactcctcc ctgcaggatc gagccaagcc
     8041 ggcggcgaag gaaaaaacgt gcccttgctc gttgtcctta tcgagaagat aaaggaggaa
     8101 ggccttgatc ctttcgggag acgccgggtc gaggccgagc ttgagggcga tggcgtcgga
     8161 cgtcttgaag ccgatccccc aaatgtcctg ggccaggcgg tagggattcg tccggaggac
     8221 gtcataggct ttcggcccat agtggcggta gattttcgtc gccagattgg tcgaaacgcc
     8281 gtggtcttgg aggaagatga tcaggccgcg gatgtcttcg tgctcggccc aggatttccg
     8341 gatttccttg tatttcacct ccccgatgcc cgcgacctcc cgcatccgct ccggcgtttt
     8401 ctccaggacc tcgagcgttt ccccgccgaa cgcgtcgacg atgcgcttgg ccaagacggg
     8461 accgatgccc ttgatcaggc cgcaggccag gaatttctcg attcccgtcc ggccggccgg
     8521 aagggaggga atgaaggttt cagccttgaa ttgccggccg aatttcgggc tcatctccca
     8581 agtccccgcc acccggagcc gctcgccggc cgaaagcgga ggaaattccc cgacgaccgt
     8641 gaggcgttcc ccgccgtcgg agacaaagcg gaggaccgaa tagccgttgt cgggattgaa
     8701 gaaaaggata tgctcgatcg ttccctcgag cgtctcggac atgccgacat cttacaataa
     8761 atccggcacc ggggccaccg agcggaggga tctccaggcc ggggattcgg gcgcgggccg
     8821 tgaatcccgt ctccgccgct tctttctttc acccggcttc gccgtctttc cctcgccgcc
     8881 cggatgcccg gttccggacg ggaacgcgag agccgggcga ccgggacgcg ggaggcgccg
     8941 gcggacgcct tcagctcttg tccgtgctct gaagcgccgg atcgaggcga ggaccgtaaa
     9001 caagctcccg gaaggcgagc tcgaaggtcg cccccgcccc ttctccctcc acgcgcaggc
     9061 ggccgttcaa ttgatgaacc agcgcgtcga tgatttggat cccgaaggac tccttccccc
     9121 gcggatcgaa ggcctccggc agcccgatgc cgtcgtcccg gacatggagg agatattcat
     9181 ccccgcggcg gcggagactg acgaagatct ctcccttccg gtttccggga aaagcgtgct
     9241 tcatcgcgtt ggaaaggaat tcgttgagga tcagccccag gggattggcc gtggtcaagt
     9301 cgaagaagac cggttccatc tcgaatcgca gcgtgatcct cgtcggatcg atggcgtgcg
     9361 actggagaag atgggtcgcc aggctccggg cgtactcgac gaaatcgatg cgggagagat
     9421 cccggctttt ataaagcttc tcgtggagca gagccatgga ccggatccgc cgctgggttt
     9481 cccgcatggc gtcccggaac cggtcctcgt tgaactgggc ggcctggagg ctcaacaagc
     9541 tcgagatcac ctgcatgttg ttcttgaccc ggtgatggat ctccttgaga aggacttctt
     9601 tttcccggag cgaggcccgg atcgcctcgt ccgccttttt ccgttcgttg acgatctcgg
     9661 tgtagacgat gatgccgccg atggccccgt cggcttcata ccacggccgg cactcccagc
     9721 gggtccaatc cgtcgtcccg tcggcccgcg gataaggatc gtcctcggcg ctcgagatga
     9781 gaccggccag gcccttttgg tagatttcgc tccacttccc gggaaagtcg gaaaacacgt
     9841 cgtccgggcg cttcccgatg acatcctttt ctttcacgcg ataatcgtcc aggtaccgct
     9901 ggctgacata gaggaagcgg agattgcggt cgtggacggc cacggcgctc cggttgtgct
     9961 cgatgatgta gcgcatcagg tcgtggaaat gggacagggc gctttccgct ttccgctgct
    10021 ccgagatgtc ctgcatcgcc ccgatcatcc ggaggggctt ccccgcgccg tcccgggtga
    10081 caaagccccg gtcgaagatt tccaaggagc ggccgtcctt gcagaggaag cggtactggt
    10141 cggaccagaa ttcctctccg ccgtcgatca ccgcgcggat gccggcgacg acgcggtccc
    10201 ggtcgtccgg atgaatgcgg cggatccaag acgtgatatt cggttccgtt tcagccgggg
    10261 aataaccata caactcgtgg aaattctcgt tcagccaata ggtccccgtc tggagattcc
    10321 aatcccagat gatatcgaag gtggccttgt tcgccagctc gaagcgctcc agcgagtcgc
    10381 gcagcttttg cccgatttcc gcccggctgc ggaaaaattg gagccgttgg cggcgccaga
    10441 tcgtcctcag ggccgcgccc aaggccgtga tcagggcggc cagcatcagg atcatttgcc
    10501 acaaccgctc ccgggccggg gcgttgattt ccgccaaatc catccggacc gtcaaatacc
    10561 agggggattc cgggaccgcc cggagaaagg ccaggaccgc cgttccgtgg tcgtctttcc
    10621 cctcgaagat cccctcccgc cccaggacgg cctgaacagc cggaatttca gtccgggtca
    10681 gggggagacg gaggttcaag gccgctccgg gccgaaactt gggatcgctg acgtaaagaa
    10741 ccgtatcgcc gtcgcggcgg acaagcagcg tttcagcgct ggcgctcggt cccggccagt
    10801 tcaggacata gggataaaga tgcgcggccg gatcgatcca gaaaacgacg gctcccagcg
    10861 gccgggaagc gcttcccggc cggtaaagga cgaggggaac gtacagggaa aggtggatcg
    10921 attcctcgcc ctcgcggtga aaatcatgga gaacggcctg gttcgcggcg atcgcctcgg
    10981 ccaccgcgat cggactttta ttgaaggcct cgtgggcccg gggaggcagg ggaacagtca
    11041 gccgggcggt cccctccatg tccacgacgt aagccctgag gtattgccgg taagcctggt
    11101 accgcagaag ccgggattga atctcgtccc gggcttcgaa gtcggaggga cgggagagaa
    11161 atctttccac gagctcggcg aaataggaat ccgcctgaag aagagaggcg tcggccatcc
    11221 gctcccgccg ccaatccctc agctcgtcgg ccttgagccg ggagatgatg gtcagttgat
    11281 tttcggcttg gacgcggaga ttcgactgga aggtccggaa agagacgtaa cccgcggccg
    11341 tgatgcccga ggccagcacg atcaggatga aaagaaaagc cgccagggag cgatcggccg
    11401 agacatcttc ttcgccggtc tccccggccg gcgcccggtg gaagaggcgg aagggaaaaa
    11461 cggcgatcgc caccaggagg aagctcaccc ccatgccggg agtgtacgga ttgaattccg
    11521 cctgcgggaa caggttgaac gcggagacgg cggcattcgc cacggcgacg aaggccgcgg
    11581 cgttgatgaa gaagaccggc ccaacgcttc gagatcgctt tttcagcaag gcgaaggtga
    11641 ccagcgccat tccggcgaac agcaggatca agccgtagaa ggaatgcacc aggaaccaga
    11701 cgccggccgt ccggacaccc gtgtcggcga tccaaaaggg tcccttgagg acgaatccga
    11761 cctcccgctt gacgaagaga tgatggcggt cgttggtcca aaccatcact tgggtgatca
    11821 cggggatgag aaaaaggccg gccgcgagcc gccggccgat ccatttcttc cggccgctgt
    11881 aatccagggc gaaccagatc cagaacacgg gcatcagggc gctgaagacg aaccggatct
    11941 tgaaccagaa cagcgcctgc ccggcggagc cggcgaaaag agaaaggatt tcagcgaagg
    12001 cgagaagcga ttgagcgacg gccagccaaa aaaacgcccg ggcccccggc agatccctcc
    12061 gccgccggag ataaccggcc agcaggccgg ctaaaataaa cgtcagggcg agggcggtaa
    12121 aaaaaaacaa gctcgaggcg gaatacatcg aattgattcc taattatgcc gaggctttag
    12181 cctttgtcaa gccgccgacg gccctctgac gattcgagcg gccgagccgc cgcggcgtcc
    12241 tttttccgat ttcgcccccc gccccttttc cggggacggg gggaacacga tccggccgaa
    12301 tcccgccgat cctcccgggc ccggacaagg gacgattgac cggcccccgc ggggcgtgtt
    12361 aacgtgaaac ggcgatgcgc gtcctccgcc ttttagccaa cgggctgatc tgcgggatct
    12421 atttctgctt cctgctcgcc cttctggtcc tcgacctcaa catcaaccgg ccggcggccg
    12481 cttttgactt cgtccgcctg gccgtcttcc tcttcccgac gtacgggctc ctgtccgccc
    12541 tggcctcgat tctcaccgcc tctctctacc ggtttttcgt cacccggcct tcggaacgga
    12601 attttctctc gccccggttt ctcaccctgg gcttttcgct tctgacgctc attttcctcg
    12661 tcgttttccg tgaaaacacg atccatttcg cctcgttctt cactccggcc gtccgccggt
    12721 ccgttcaaat gcaagcggcc gtcctgttcc tggcggcctc ggccggcctg gtgcttctct
    12781 tccgctactt ccaccggagg cggcgggccg ccttcctcgg cgcctcgctc gccgccgccg
    12841 tcgtcgccct cgccgccgcc tgtcttctcc ggatcgcata cccggccgcg gacacgccgt
    12901 tccgcctgtc cagcatggaa gcgaagccga tcgcccggag agtgacgatt ctcaacctcg
    12961 agggcttgag cttcgatttc atctatccgc tggtgaaccg gcggcttctg cctaatttcg
    13021 cctacctgat ggaaaacggc tcctgggggc acctggcggg attcacgccc agcgacacgg
    13081 ccgttctgcg gcgcaccttg aaaaccggca agcttccggg gaagcaccgc cgcctgtccg
    13141 acgtccgcta tacgatcccg ggactccccg gacacctgga agccgtgccc cggttcattc
    13201 tcttccgcca gctcaaacag ctcggccttc tgaaaatcga gccgtacgat ccgccgccgg
    13261 cggtcaagga catcgagatg atttacggcg aggagcaggc cgtcgtcgtc cgttcggagg
    13321 aacgaaccgc tctcgccccc gagacgctcc cggaggcgcc ggccgccgac aagctgttcc
    13381 tctccctcta caaggacctc caagacgaga gagctccgat ctgccgccgg ctggaggcgg
    13441 ccctgatccg ggaccggatc accgaggaac gggccttccg gtcgaaagcg gaactccccc
    13501 cccagctctt cctcctgacc ctggacggcc tggatgacgt ccaaacctat ttttacaaat
    13561 acagcgtccc cgcggagttc ggcgaaattc gtccggacga aatccagaag ttcggcacgg
    13621 tcatcgagcg ctactatcaa tactacgacc agatcatcag caaatacacg gcggcgatga
    13681 aggacgatga aatcctcatc gtctgctcgc cgttcggcgt cgcccctctc cccttctgga
    13741 aacggctggt ggagtgggtc ttgggcaacg ccgacgtctc ggcttaccac gaacaggcgc
    13801 ccgacggggc cgtctttttc tacggatcgg gcgtttcccg gggacggagc gccgacggtc
    13861 tccgcctggc ggatctcgcc ccgacgctcc tgtattacgc cggcctgccg gtggccaagg
    13921 atatggacgg attcgcccgg acgacgatct tcacccggga attcaaggag agaaatccgc
    13981 tcattgcgat ctcgtcgtac gaggacgtca ccatccggaa gcggcgttga tggaaaagga
    14041 aggccgggcg gggcgccggc ctcagcggat gattttcagc atccggttcc agcgggcctt
    14101 ggggatgaac cggacgagct tcttcagccg gtcgcggacc gaggttttca agtaagcgtc
    14161 ccgctccgcg gcataggaga aatagatgat ccagggacgc cccttgacat agctcaacgg
    14221 gacgaagccc caataccgcg aatccgagct ctggtcccgg ttatcgccca tggcgaacaa
    14281 gtgtccgggg ggaatggtca ccggcccgaa attatcccgg atggtgtcct cgtaatgata
    14341 gtagccgttc ttggcgaaaa cctgggcatc gctatggacc ttgaacggct cccggagggg
    14401 ctgcccgttg atgtagacct gcttgtcccg gacctcgaat ttttccccgg gaagcccgat
    14461 gacccgcttc acgtagtcct tggacagctc gttcggatac ttgaagacga tgatgtcgtt
    14521 gcgcttgatg tcgcgccgcg ggaggaggag gcggtcgagc ggcgggcttc cgttgtcata
    14581 gacgaatttg ttgaccagga gaaagtcccc gaccaggaga gtcggctcca tggagcccgt
    14641 cggaatctgg aacgcctgga cgacgaaggt catcacgaaa aaaacgaaga cggccgtctc
    14701 cgcgatgagc tcgaaatact cgcggaagac gcttttcgtc cgcttctttt ttccgtccgc
    14761 cttctccttg gtcatcgcct tttcctttaa aagagggata ctatagccca aaatgtcccg
    14821 ggcttcaacc gaaggagcgc ccgccgtgac ggcttgacga tcatgtcccc cgtaacggcc
    14881 tgacgaaaat atccccttgg gcggaggcgg acatgccgga gagaagcccc gtgagccgac
    14941 aggaggcccg gcggaggcaa gggcttgaac agggcgtaaa gaggaccccg gcgcttaacg
    15001 cggcggatcc cggagccgtg tcgcggaccg gacgtttggc cttcgacggc gaatccggta
    15061 tacttccccc catccttata tatatcttgt attgatcccg ttcatgaaaa aacgcttcat
    15121 cgtcgaaaca acggccgccg gctcctcggc ccgggctgga ataatccgga cgccccacgg
    15181 agacatcccg acgccggctt tcgcccccgt cgccagccag ggcgcggtca agggcttgac
    15241 ccatgaccag gtcacggccc tcggagccca cctgatcctc tgcaattcct accacttgtt
    15301 cctccgcccc ggagccgaag ccgtccgggc gatgggcggg ctgcatgcct tcatctcctg
    15361 gcccaagccc attttgaccg acagcggcgg tttccagatt tacagcctgg aaccgctggc
    15421 caaggtccgc cgcgatgggg tggccttcgc ctcccacctg gacggctcga agctgttcct
    15481 gacgcccgag gacgttgtgg acatccaagc gcttctcggc tcggacatca tgatggtcct
    15541 tgatcatttc gtccctttcg cctcccccga atccaaggtc cgggaggccg tggagacgac
    15601 gatccggtgg gccggccggg cccgcgaagc ttttcggaaa agggaatccc gggcgcatct
    15661 ctgggccatc gcccagggcg gcgtcatccc ggagttgcgg cgccggtgcg ccgaggagct
    15721 gagggacatg gatttcgacg gctacgccgt cggcggattg ggcatcgggg aggccaaggc
    15781 ccaacttcac aaaacgttgg aacaaagcga cgcccttctg ccgaaggaca agccccgcta
    15841 tctcatgggc atgggctatc cggccgacat cctcgaggcc gtcgaccggg gggtcgacct
    15901 cttcgactgc gtcctcccca cccgcaacgc ccggaacgga tcgctgttca cccgccgggg
    15961 cgtcgtgacc atcaaaaacc ggaaatacgc cgacgacctc cggcccatcg acgaagactg
    16021 cggctgcccg acgtgccggc gctattcgcg ggcttacctg cgccatctct acgaacgcaa
    16081 ggaaatcacc tcggccgtcc tgaacacgac ccacaatctc catttttatc ttgacttctt
    16141 caaggaaatg cggcaatcta ttcaatcgca ttcgtttcag caatttaagt tgaatctaat
    16201 gaacacgtta aaggaagtga accgatgatt ttcagcgccc ttttcgcggc tcaacaaggc
    16261 caggttccgg cgggacaggc ggctccccaa ggcaacatgc tgaccgccct gattcccttc
    16321 gtcctcgtct tcgggatttt ctatctcctc atcattcttc ctcaacggaa gaaacagaaa
    16381 aaacaccagg aacgaatcga agctctgaag cctggagaca aagtcgtcac gacgggcggc
    16441 atcttcggca cggtcatggg cgttttgaag gaccggatcg agctcaagat cgcggccaac
    16501 gtcaagatcg agatcaccaa gggggccatc ggcgtcattc tctcccaggg agaggcgtcc
    16561 ccggccgaaa agccggaatc ctgaggaatt cgtgaactcc ggccggccgt ctccgggaca
    16621 ccggccgcgc ccgcgccctc cggcgccggc ccatattatt aaggaaagag acaaccgatg
    16681 aagaaaggac ttcgctggaa agtgcttctg acgctcggcg cgctgggcct cagcctgttt
    16741 ctcgcccttc cgctcaagga aaaaatcaag ctcggcttgg atctcaaggg aggcatccat
    16801 ctggtcctcc aagtcatgac cgacgacgcc ctcaacggcg aaaccgacca ggaaatcatc
    16861 cgattcaccg acctgttcaa gaaaaactcc atccggttca cgaccgcggc caaggaaaag
    16921 ccgggccgct tcgtcttcac cggcctggat gtggatcaac agatcaaggc ccgggacctg
    16981 gtcgcgcagt acacccgcga ctgggacgct tcgttcatgg ccgaccgggt cacgtttacc
    17041 ttgaaggcgt cggccgtgac ctttctcaag gaccaggccg tcgtccagac agtggagacc
    17101 atccggaacc gcatcgacca attcggcgta tccgagcctc tcatccagcg ccagggggag
    17161 aaccagatcg tggtcgagct ccccggagtg gacgatcccg agcgggtcaa ggatctgatc
    17221 aaggtcacgg ccgtcctgga atggaagctg gtcaaagccg gtccggccga ggatgaggcg
    17281 accctgcttc agtccttcgg cggaacggtc ccggatgacg ccgaaatcgt ccggggcgac
    17341 ccggcccgag ggcatcccgg cgtctacttg gtcgaaaagg tcgcaaccgt cagcggcaag
    17401 gacctgcgga tggtccgccg gggaaccgac gagtggggca atccggccgt ttccttcact
    17461 ctttcctccg acggcggacg ccgtttcgaa caagtcaccg gagcgaacat cggccgggct
    17521 ctggccatca tcctggacga ccgggtgcaa tccgccccca acatcaacgc ccgaatcggc
    17581 gattcgggga tcatccaggg acagttcacg atccagcagg ccgacgacct cgtcgtcgtc
    17641 ctcaaggccg gggcgctccc ggccggcatc aagtacctcg aggaacggat gatcggcccg
    17701 gccctcggct cggattccat ccgccaggga atggccgccg gcctggtggc catcctcctg
    17761 gtcatgacct tcatggtcgt ctattaccgg ctctcgggtg tcaacgcggt gatcgcgatg
    17821 atcctgaacg tcgtcatcct cttcggcgtt ctggcttatt ttaaagccaa cctgactctt
    17881 cccggcatcg ccggcctgat cctggcgatc ggcatgtcgg tcgacgccaa cgtcctcatc
    17941 tttgagcgga tcaaggagga acgggccttg gggaagaacg ttccgggggc gatttccctc
    18001 ggcttcagcc gggctttcac ggccattttc gactccaacc tgacgacgat catctcggcc
    18061 gtcttcctct tccagtttgg aaccgggccg atcaagggct atgccgtcac cctgatcatc
    18121 agcctcgtgg ccaacatgtt cacggccgtc ttcgtctccc ggctgatctt cgacctggtc
    18181 attcccaaaa ccgccgtaaa gttgagcatc taacatggaa atcttcaaga aaacccccca
    18241 gatccgattt ttaaaatacc ggggcatcgc cttcatcgtt accatcgtca tcatcgccgc
    18301 cgggatgctc aacatcttcg ccggccgcgg cctcaagctc ggcgtcgatt tcggcgaagg
    18361 aaccctgatc cgggtcatgt ttaaaaaccc gaccgacgtc ggggacatcc gcgacgtgct
    18421 ggccgacatc ggcctgggaa gcagcatgat ccaagaactc ggccggggcg gccgggagtt
    18481 ccagatccgg gccgtcgaag tgaaaagcac ggttgaggaa agccggctgg agagccacga
    18541 ggtcctgggc aaccggatca tcgaagccct caagggaacg gacggccaaa acgaaaccgc
    18601 ccaggggctg gctgatctta acatgatcga ccagaagagc ctggccgccc ttctcgaacc
    18661 ctccttcctc gaacgaagct ccgggctggc cgagcagatc ctctccgtcc gcaaaaacga
    18721 ccggaaccag ggcatcttca aggattacgc ggatctctcc gccgacggcg tcccggccga
    18781 ggtcgtgact tctctgaagg gcaaaacgta tctcggcaag ctgaacatcc tcagccgcga
    18841 atccgtcggc cctcaggtcg ggaaagacct ccggaagaaa gctctcatgg cgacgctctg
    18901 gtcgctcgtc gggatgctga tttacatcgc cgtccggttc aagatggcca acggagtggc
    18961 cgccatcttc accctgaccc aggacgtcct gatcacgatt tccctctatt ctttcagcgg
    19021 ccgcgagatc aacctgccca tcgtcgccgc cctcctgacg atcgtcggtt tctcgatcaa
    19081 cgatacgatc gtcattttcg accggatccg ggaaaaccag aaagccaacc ggaaaatgcc
    19141 ggtcgaggac gtcatgaacc tcagcctcaa ccagatgctg ggccggacgt tcatcacgtc
    19201 cggaacggtc ctcatgaccg ttctggccct cttccttttc ggcggcccgg tcatcaacga
    19261 cttcgctttc tgcatgctca tcgggaccgt cgagggcatt tactcgacga tttatcttgc
    19321 ctgcccggtc attttggcgt ggcaaaaatg gtttccgtcg aagcgctccc ggcggtaagg
    19381 cgggattcag ccaggggcga ggatttgact tgctaagaga aaacaattgg tttataatgc
    19441 ctcccgtcga ttgctgatta tccatcactt tatacgaggt acgaaatggc tgaacaaacg
    19501 aagccgacaa gcccggtaaa ggaaaggacc gcggatattt tcgacgaatc cttttttgtc
    19561 gccggaaaag ccgccggacg aaaggccctg gccttccccg tcgccgtcgt tcttcacctg
    19621 attctcgtgg ttgcgttggt cgttcttcct ctcctgtccg tttcgaatct tccccaaatc
    19681 gagatcacga gcgcgttcct ggcgcctccg ccgccgcctc c
//
LOCUS       DAOOXH010000043        19294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_2725_length_19294_cov_7.959094, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000043 DAOOXH010000000
VERSION     DAOOXH010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 19294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19294
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2725_length_19294_cov_7.959094"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKV64_06500"
     CDS             <1..516
                     /locus_tag="PKV64_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16766.1"
                     /translation="WVRLAVLAIFVLAALSTASAQSLHAKWFKVLVKADTCRVNPYTG
                     FFSSYKFQFYIYVYLHYAGPGESPRGSLYDWEVWSKTEGGRWECVMEFSESSSSQSEN
                     FFPDINMSLPTEKGDNFSTYVTPRIVASPLSNTFVAGGEIYAGRDINGRRLYGWLTMT
                     GKLVPMPKWVD"
     gene            666..1673
                     /locus_tag="PKV64_06505"
     CDS             666..1673
                     /locus_tag="PKV64_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPN16767.1"
                     /translation="MKEDAARLISLVDRFPLLRIAVWGDFILDEYLYGFTRRISREAP
                     VMVISYRNREYALGGAGNAAYNLAALGVEVEAVGVVGRDETGRDVRRLLRRAGIADGS
                     ILAEQEFATPLKTRILAGEENTKKQQILRIDREGEVPGIKPLHNKMGSLLKKAAGSCD
                     ALLVSDYHSRTVTEGVYETARDAFRAAGRPVAVDSRFRLVRFKGCTIATPNEPEVEIQ
                     MKTAFEGGTAVLIAAGRALQRKLQSPALLITRGSQGMALFERGKPPYLLPVFGSTDIV
                     DVTGAGDTVIAVFTAVLAAGGTFREAARLANFAGGLVVMKKGTAVVSSPELKQAVLVG
                     N"
     gene            1670..2179
                     /locus_tag="PKV64_06510"
     CDS             1670..2179
                     /locus_tag="PKV64_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HPN16768.1"
                     /translation="MKTAKILSLSALRRIADEARARGLRVVLANGGFDLIHAGHIRYL
                     REARRKGDLLVVALNSDASLAGLKGPGRPILPQRERAEIIAAVEGVDYVVIFDDPDVG
                     RILRALRPDVHVKGSDYTKETVPEREIVRSYGGKVAIAGGPKIRSTSDIISRILNFPK
                     DGRRPRPSR"
     gene            2176..3162
                     /locus_tag="PKV64_06515"
     CDS             2176..3162
                     /locus_tag="PKV64_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPN16769.1"
                     /translation="MNEYLIIRLSSLGDIVHALPAFAALRRREPEARISWVVEPKGRE
                     ILDLVEGLDEIIAVGEPGWTEKLRRRDRIALDFQGLLKSAWIGWRSRSRRRLGFSAGN
                     LREPAARWFYTETAPPLSEDDHVILKNLRMLELLGYKETLVRFPLSVPAALVQAAHER
                     LSALGWNPGLRPVIFNVGAAWPTKRWFPERWTETLRRLDRAGLFPALLWGTEEERRTA
                     EDVGRASGVPVLPFFSVRETFGLLKISALIVSGDTFALQAAAAIGLPAVAVFGPTNPR
                     RNGPFTPRDRVVYHELPCGRCYRRACPDPKCLKAVAVDEVLEAVREALASSE"
     gene            3164..3706
                     /locus_tag="PKV64_06520"
     CDS             3164..3706
                     /locus_tag="PKV64_06520"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPN16770.1"
                     /translation="MTLREILYRWRVRAALFAFVAVLVLARPTAASIAAGAAIGLAGL
                     AVRGWAAGHIRKDKDLAVTGPYRFTRNPLYVGSFILGLGLVCCAWTWWGAAIFGLYFL
                     VFYVPVMAEERDRLRRLFPQAYAEYEKRVPLFFPTLRGAAPSEPRKWEAALFRRNKEF
                     RAWLGTAIVWGLFILRGWIR"
     gene            3703..4287
                     /locus_tag="PKV64_06525"
     CDS             3703..4287
                     /locus_tag="PKV64_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPN16771.1"
                     /translation="MTRTPGRLARLRTVLGRRQTDLRIVLEEITNAHNAAAVLRTCDA
                     AAILHVDLIQAEPAPLPFNQAITTRADKWLVLHHHPSPAACYETLKAAGFQIAAADLS
                     PKAIPHTELDYCRPTAIVFGSEREGLSPAARDGADVRIKIPMFGMVQSLNLSVSAGII
                     LYEAVRQRLARGFFDSPRLSPEEFEKLLAGWMEL"
     gene            complement(4313..5887)
                     /locus_tag="PKV64_06530"
     CDS             complement(4313..5887)
                     /locus_tag="PKV64_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPN16772.1"
                     /translation="MSHRHFLFKAALLALVLAAGSLSAVAAAVPADERNIESILKSTA
                     PSVVKVEARNGVRKIATGVVIDAEGTIVTTALISPRDEKITVRTFDGKQYPADFKGFD
                     TLTGLAVIEVKNKGLAPIALGTTADARPGVWAGAVGFSPEDTPAVTQGIVSSATADRI
                     RLNLWVMPGSSGSPVVNSEGRLIGVLRGTFFEDQPVYFEFREQQVVGTGTVFSRAEGP
                     SSGMALAVPADVVRSVANDIRKDGKVLRGWIGIMIAEKDGRLEIREVEPESPAEAAKL
                     KPGDVIVKAAGAEIPSGAAFSREIRKRKPGTEVVLEILRDKESKEVKVKLGEFTPEDG
                     RLELETRFPRLFPPLVKGTERPAAPGGPERFFVWENRRYIGVTLQELTRDLAAFFGVK
                     DGRGLLVTEFAPDSPAQKAGLKAGDVILKADGRTMDTVARLSDLIRDKKAGDKVKLEI
                     LRDRKPMTIEIPVAEEDKRPEGAASVFRGAVRDLRDLFGDRPLGTLSREQMRRLAEEF
                     EKEGTRPPRLRGSYRI"
     gene            complement(5898..6272)
                     /locus_tag="PKV64_06535"
     CDS             complement(5898..6272)
                     /locus_tag="PKV64_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16773.1"
                     /translation="MDDAKAWKGLARVAAPEDFEDRVLRELDRRRRARPQERRVRLFK
                     WALSGSAAALLAFFTILNFVVPGKEPRTAGGYAVSAAEPSAIPVMETIDYGREIRAGS
                     TDAEAVYLLEQISDSSFAMIRY"
     gene            complement(6259..6846)
                     /locus_tag="PKV64_06540"
     CDS             complement(6259..6846)
                     /locus_tag="PKV64_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPN16774.1"
                     /translation="MSMDDQALIARVQQGDGSAFAEIVRAYKDKIVNYLYQVTSDYQK
                     AVDLSQETFIRVYFKADRYRPIAPLSSWIYAIASNLAKTDHKRRRRMMAVSLEDLPPT
                     VEISTPAGEESDTGLTRNLRAALAALHPRYRIPVVLKDVEGYSQEEIAAIVKSPVGTV
                     KARISRGRAMLRKSLEGASDGVTAFAKREISDGRC"
     gene            6990..8102
                     /locus_tag="PKV64_06545"
     CDS             6990..8102
                     /locus_tag="PKV64_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HPN16775.1"
                     /translation="MNGTKERSILFSPLKVGTAEIPNRFVRSATHEFLAGKDGRMTER
                     HRDLYRALAEGGVGLIITGHAYVNRAGIASPGQTAADDDRLIELFRPVPEAVHGTPSK
                     IFLQIAHAGRQTKERLTGGVPLAPSAVPDPVSKVTPREMTEGEIHGLVRDFIQAARRA
                     AAAGFDGVQIHAAHGYLLSGFLSPHTNRRTDAWGGSTENRARVVVEILAGIRAELGRK
                     FPVIMKINASDFLPSGLEPEEAIAVARQLEPLGLDGVEVSGGMAEAGRGSVWPGLRTE
                     DEEGYFVGDAARFKAALKIPVFGLGGIRTFRVMERFVQEGRVDFISLSRPLVREPDLV
                     RRFRLGQAERSACLSCNKCFNPRGLSCGDLRKRPSP"
     gene            8408..8914
                     /locus_tag="PKV64_06550"
     CDS             8408..8914
                     /locus_tag="PKV64_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPN16776.1"
                     /translation="MLVDLFSKKVFNLSYQFAGSYQEAQDLTQEIFVKLFHALPKYEP
                     GRNFSAWLTTLAKNHLIDKYRRTRWEKKNRDDFEDHLFSAAAPDGDPEADLLRGERRR
                     ILWEGLNQLPPDIRLAVLLKEIHGKSYGETAEITGVPEGTVKSRVNRGRLQLARILQK
                     TREKADVL"
     gene            8904..9398
                     /locus_tag="PKV64_06555"
     CDS             8904..9398
                     /locus_tag="PKV64_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPN16777.1"
                     /translation="MSCNRIEELLPGFLDGDLGPGERAMVEDHLRTCPACASLLALLG
                     ETDAALASFPEVEVGDDLRERLAAIAGPIRRFSLFAWLRTPAFQPVLAAVTAVGLAVS
                     LYFLNPGRRDFEKKVVRTFHRGVGQVERIYARTGFLAGSIESTAENLYASLRSMKSPD
                     RDKD"
     gene            9424..9915
                     /locus_tag="PKV64_06560"
     CDS             9424..9915
                     /locus_tag="PKV64_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HPN16778.1"
                     /translation="MADPIPTEKRPSKSPAVAGILSVVLPGAGALYNGLYTKGILYIL
                     IFAGLITMQDRGGQPFFALMLAAFYFFQIIDSVNNAKAVNKTAAGGAAASEEPVPAPA
                     PLSPSPSGSIFWGVLMIALGGIFLLGNFDVIDFDRLWDFWPVIVIVIGVKMISDYFSR
                     KNA"
     gene            9931..10149
                     /locus_tag="PKV64_06565"
     CDS             9931..10149
                     /locus_tag="PKV64_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HPN16779.1"
                     /translation="MAKRKDPLIWGLVLVALGLVFILENFHVDAWDFVWRFWPVVLIV
                     WGATKLVDGLKSKSRRDDAPLPPAPPQA"
     gene            10151..11464
                     /locus_tag="PKV64_06570"
     CDS             10151..11464
                     /locus_tag="PKV64_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16780.1"
                     /translation="MRGREIALAVLLIALGVLVTYEKSGRLDGWFQSPDGPWFGRDEY
                     VYEESRDIDGPPPAEIQVINAHGRIEVTGTAESGPVRVVFRKHVYARNKTEADRVAAA
                     LRMKAAPSGPRLILETNRSEFERKRFSTAFLLAVPAGTAVVLKNSHGLVRAEAVASAE
                     ISNPNGDVVVRDLAGPLVLSSSYESVDVAGVGGNARIDAPHSRVVLKKIKGGLIVSHS
                     YDKIDLEEIDGPVSVEGTHSKVTARGLASTAGISTTYEAVRVEDAGAVTIRARHSDIF
                     AKNIGGTLDVTNTYGRIRLENIEGELKIDGRNVAVEGRGVRSSDVFVQTTYENVFLAG
                     GLGKISVALTHGRLRLEPEAALTGPVDVRGDYADVRLLWPAGFRAPFQARIRDGRMIW
                     NLPDPLDSEKSNGVTEVRAFSGAAGNPGVTIETAHGDVQVDAAGR"
     gene            complement(11471..12367)
                     /locus_tag="PKV64_06575"
     CDS             complement(11471..12367)
                     /locus_tag="PKV64_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16781.1"
                     /translation="MNEVEELLKIDLKDLPPIPSLVLDDLHQKVLKNLYLELGSGPVL
                     HLLTPSYSHPSSTPSPAVSELVTKKEGLLDYLKELLVQNLAVYSALVQVNSYFIEQNN
                     FLVLARLGERDSGGRRYEVKYYTHSPVELLSHYEDKIYIGRDFIDLYNFRRKYFGVRE
                     LIASLKTQSEKLIDRAEAKLKKPLEYKSFFQEIRESVGEINSESLAILESLPPHLEAA
                     KVSEIDLIEINAQYRAIAHYLIELNDEVGEFENLLRFKMEIGFVRYVTKFKKDLTNLI
                     NLFNIKINGALSARILCPRSKP"
     gene            complement(12444..13253)
                     /locus_tag="PKV64_06580"
     CDS             complement(12444..13253)
                     /locus_tag="PKV64_06580"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HPN16782.1"
                     /translation="MMSGKKWRLIVDRVPTAGDWNMAVDEFLFRSLTDDPSTIVRFYR
                     WERPTVSLGAGQTTARVVDLEFCRRNGLGVVRRPTGGKLVLHDREVTYAVVSSDDGTF
                     TSTLGGSYLWISQALVKGLESLGLKPSLAAETPSGYAKGTMPCFSRPARDEIEIDGRK
                     IIGSAQKRIGNRFLQHGSIPLAKDEELLRAVSLRKEDPAGVIRMTSLSEALGRPVGFD
                     EAAGRFIEGLAAFGGVEFEARSLEDAERAAIEDLRRRKYGTEAWTIDRREP"
     gene            complement(13280..13351)
                     /locus_tag="PKV64_06585"
     tRNA            complement(13280..13351)
                     /locus_tag="PKV64_06585"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13317..13319),aa:Gly,seq:ccc)
     gene            complement(13549..13734)
                     /locus_tag="PKV64_06590"
     CDS             complement(13549..13734)
                     /locus_tag="PKV64_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008579483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN16783.1"
                     /translation="MTGGVLIASDLPFSKREGIFKDPMVTAAAVDRLVHHSVILGLNN
                     RSHQRPLSALINIYAKQ"
     gene            13813..14181
                     /locus_tag="PKV64_06595"
     CDS             13813..14181
                     /locus_tag="PKV64_06595"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPN16784.1"
                     /translation="MSRADESRTAAPVIPPDIPRLSPGVVFEGELASSEDILIQGEFK
                     GTLRLLPQASVYIDRGARVEASVAAGNVYVFGTLIGPARASACVFLSAEAEMKGDISA
                     ARLFVQEGARFRGSVQTTAD"
     gene            14244..16232
                     /gene="uvrB"
                     /locus_tag="PKV64_06600"
     CDS             14244..16232
                     /gene="uvrB"
                     /locus_tag="PKV64_06600"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPN16785.1"
                     /translation="MDAFELVSDFKPKGDQIRAIEDLSEGLDRGLLHQVLMGVTGSGK
                     TFTVANVIARANRPVLVISHNKTLAAQLFQEFRRFFPRNAVEYFVSYYDYYQPEAYLP
                     SSNTYIAKEVTINDEIDRMRLNATNRLFERRDVIIVASVSCIYGIGAPETYYAMSFPL
                     AAGERGPRKRIIQALVDIQYERQEDDFKRGGFRVRGDVIDVFPAYADFAYRIELDGDA
                     VGRITSIDPLLGRQREEFSKIIIHPRTFFSTPRDILEASVDGIEKELKQRVDDLRTRG
                     RIIEAERLEERTLFDLDMLREFGYCPGIENYSLYLSRRKPGQPPYTLLDYFPRDFLTI
                     IDESHVTIPQIGGMYHGDRSRKETLIEHGFRLPSALDNRPLQFREYEERVGPVLYVSA
                     TPAPYEIKASRKRIIEQIIRPTGLIDPEIEIRPVAGQVEDLRREIERRAARKERVLVT
                     TLTKKMAEDLTRHYHDLGLRVRFLHAEIETLERVVLLRDLRKGVFDVLIGINLLREGL
                     DLPEVSLVAVLDADKEGFLRSTTALIQTFGRAARHVEGRAILYADVLTDSIKAAVAET
                     NRRRALQAAYNAANGITPRSIRKEIDEGLASAAERDYFDYTRIAEDRAVFLSPERRKR
                     KIEELEKAMKTAARDLDFEKAAGLRDEIARLKKGELAI"
     gene            16243..17859
                     /gene="uvrC"
                     /locus_tag="PKV64_06605"
     CDS             16243..17859
                     /gene="uvrC"
                     /locus_tag="PKV64_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPN16786.1"
                     /translation="MSHPLKEAAAGLPDKPGVYLFKNARGEPVYIGKARSLVDRVKSY
                     FQPTDDPKVRNILAETVRLDFILTASEREAAFLENNFIHHLQPKFNLRLKDDKSFPYL
                     RLMVRDSVPGLFFSRKVDASDGARYFGPFSPAREAGKTLQLLTKSFRLRTCAEAVFRT
                     RRRPCLEFEIGNCSAPCVGKISPEDYAASVRDAVLFLEGRTRELAGILRGRMDAAASA
                     ERFEDAARWRDLLRTVEQIRSRPKAISVKLENQDVIGLAQAGALQAFCVFLMRDGKVR
                     ESVSLIRERDAGPSPEKALRDFLEDHYSGRPLPDRILVPALPEGAAALRARWKTAAKR
                     RVGLVVPRRGDSRTLLDWAVKNAESLLRRADGERAAVEDLQSALGLQSVPVRIDGFDV
                     SHTQGVETIASLVTFRGGRPAKEGYRRYRIRTAGPADDTAALAEALERRYGRVLREKQ
                     ILPDLVLIDGGLPQLGAARAVLDRLGLNGLPAVSLAKREEILYTPDRPGGIRLAPTSP
                     ALKLAQYIRDEAHRFALALHRKRRADRSLK"
     gene            17915..18262
                     /gene="acpS"
                     /locus_tag="PKV64_06610"
     CDS             17915..18262
                     /gene="acpS"
                     /locus_tag="PKV64_06610"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPN16787.1"
                     /translation="MILGTGVDMIEVARVQKAIERNPRFVDKVFSPLEITYCEGKKNR
                     FQHLAARFAAKEAFIKALGRGVPWTSVEVVNLPSGQPTLSVRDGKDYGFSRAHVSLTH
                     LADHAIAVVVLER"
     gene            18333..>19294
                     /locus_tag="PKV64_06615"
     CDS             18333..>19294
                     /locus_tag="PKV64_06615"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPN16788.1"
                     /translation="MNKTRVRNIWTGLLVLVLAGPVLADEGLWLFNMPPAEILKAKYN
                     FVPSPEWLDHLRLSSIRFGGASGSFVSPDGLALTNHHVGQGAIQRLSTPERDLMKTGF
                     YARTRAEELKVPGLELSVLQSIEDVTARIKGVERPEMTAAEAAEARDREIAALEEEES
                     EKTGLRCAVVNLFSGGMYHLYRYKIITDVRLVFAPDYLIAFFGGDQDNFTYPRYDLDI
                     CLFRLYENDRPYPTPHYLKWNTSGQKEGDLVVVSGHPGSTGRLLTVSQLAFLRDVAYP
                     WTLANYERRRAGLQYFSKRGGEAARNARGPLFGIENSLKAVTGY"
BASE COUNT         3411 a         6416 c         5846 g         3621 t
ORIGIN      
        1 tgggtccgct tggccgtcct ggcgatcttc gttcttgccg ccctgtcgac cgcctcggcc
       61 cagtctctgc acgccaaatg gtttaaggtc ctcgtcaagg cggatacctg ccgcgtgaat
      121 ccgtacacgg gattcttctc atcatataaa ttccagttct atatctatgt ttatctccac
      181 tacgccggcc cgggcgaatc gccgcgcggt tccttgtatg actgggaagt ctggtcgaaa
      241 accgaggggg gaaggtggga atgcgtgatg gagtttagcg agagttccag ttctcagagc
      301 gaaaatttct ttcccgacat caatatgtcg ctgcccacgg aaaaaggcga taatttttca
      361 acctatgtca cgccccggat cgtggcctct ccgctcagca acaccttcgt cgccggaggg
      421 gaaatctatg ccgggcgcga catcaacggg cggcgtcttt acggctggtt gacgatgacg
      481 ggaaagctgg ttcccatgcc gaagtgggtg gattgatcaa cactccgcgg ccggatcggc
      541 cgctctgaac cggatagagc cggattgagg aagagggccg tccatttccc cgggggcggt
      601 cttcttcctc cgtttcgttg acctccggcc cgcttttgaa tcataatgca cgtcgcaggt
      661 ccacgatgaa agaagacgcc gcccggttga tctccctcgt cgatcgtttt cctctgcttc
      721 ggatcgccgt ctggggggat ttcatcctgg acgaatatct atacggtttc acccgccgga
      781 tttcccgcga agcccccgtt atggtcattt cctaccggaa tcgggagtac gcgctcggcg
      841 gggccggcaa cgccgcatac aatcttgccg ctctcggcgt cgaggtcgag gccgtgggcg
      901 tcgtcggccg ggacgagacg ggccgggacg tccgccgcct cctccggcgg gccggaatcg
      961 ccgacggatc gatcctcgcc gaacaggagt tcgccacccc cctcaaaacc aggatcctgg
     1021 ccggggagga aaacacgaaa aagcagcaga ttctgcggat cgaccgggag ggcgaagttc
     1081 cggggatcaa gccgctccac aataaaatgg gatctcttct gaaaaaagcg gccggttcct
     1141 gcgacgccct tcttgtctcg gactaccatt cccgaacggt caccgagggc gtttacgaaa
     1201 ccgcccggga tgctttccgg gccgccggcc gtccggtcgc cgttgattcc cgtttccgct
     1261 tggtccggtt caagggatgc accatcgcca cacccaacga gcccgaggtc gaaatccaga
     1321 tgaaaacggc cttcgagggc gggacggcgg tcctgatcgc ggccgggcgg gcgcttcagc
     1381 gcaagcttca gtcccccgcc ctgctgatca cccgcggctc ccagggtatg gctctttttg
     1441 aacgcggcaa gccgccctat ttattgccgg tcttcggatc gaccgacatc gtcgacgtca
     1501 ccggcgcggg cgacacggtc atcgccgtct ttacggccgt tctggccgcc ggaggaacgt
     1561 tccgcgaagc cgcccgcctg gccaatttcg ccggcgggct ggtcgtgatg aaaaagggga
     1621 cggccgtcgt ctcgtctccg gaactcaagc aggcggtcct cgtcgggaat tgaaaacggc
     1681 caagatcctt tccctctccg ccctgcgccg catcgcagat gaagcgcgcg cccgcggatt
     1741 gcgtgtcgtc ctggccaacg gcggcttcga cctgatccac gccgggcata tccgttacct
     1801 tcgggaagcc cggaggaaag gcgacctgct ggtcgtggcc ctcaacagcg acgcctccct
     1861 ggccgggctg aaggggcccg gccggccgat tcttccccaa agggaacgag ccgaaatcat
     1921 cgccgccgtc gagggcgtcg attacgtcgt catcttcgac gatcccgatg tcgggcggat
     1981 tctccgcgcc ctccggcccg acgtccacgt caagggttcg gattacacga aagagaccgt
     2041 tcccgaacgg gaaatcgtcc gttcctacgg cggaaaagtc gccatcgccg gcgggccgaa
     2101 aatccgctcg acgtcggaca tcatctcccg gattctcaat tttccgaaag acggccggag
     2161 gcctcgtccg tcccgatgaa cgaatacctg atcatccggt tgagctctct cggggacatc
     2221 gtccacgccc tccccgcttt cgccgccctc cgccgccgcg agcccgaagc ccggatttcc
     2281 tgggtggttg agccgaaggg acgggaaatc ctcgatctcg tcgaggggct ggacgagatc
     2341 atcgccgtcg gagagcccgg ctggacggag aaactccgcc gccgggaccg aatcgccctg
     2401 gatttccagg gcctcctcaa gtcagcctgg atcggctgga ggtcgagatc ccgtcgccgg
     2461 ctgggatttt cagccggcaa tcttcgcgag ccggccgccc ggtggtttta cacggagacg
     2521 gccccgcctc tttcagagga cgaccacgtc atcctgaaaa acctgcggat gctggagctt
     2581 ctcggataca aggaaacgct cgtccgcttt cccctgtccg tgcccgcggc gctcgttcag
     2641 gccgcccatg aacggctttc cgccttggga tggaatcccg gcctccgccc ggtgattttc
     2701 aacgtcgggg ccgcctggcc gaccaagcga tggttccccg agcgctggac ggaaacgctc
     2761 cggcggctgg accgggccgg gctgttcccg gcgcttctct ggggcacgga ggaggaacga
     2821 cggacggccg aagacgtcgg ccgggcgtcc ggcgtccctg ttcttccctt tttctccgtc
     2881 cgggaaacgt tcggcttgtt gaaaatctcg gccttgatcg tatcgggaga cacgttcgcc
     2941 ctccaagccg cggccgcgat cgggcttccg gcggtcgccg ttttcggtcc gaccaatccc
     3001 cgccgcaacg gcccgttcac tccgcgggac cgggttgtct accatgagct cccctgcggg
     3061 cgctgctatc gccgggcgtg tcctgatccc aagtgcctga aagcggtcgc cgtcgacgag
     3121 gtcctcgagg cggtccggga agccctggcc tcctccgaat gacatgacgc tccgggaaat
     3181 cctttatcga tggcgggtcc gggcggcctt gttcgcgttc gtcgccgtcc tcgtcctggc
     3241 ccggccgaca gcggcctcga tcgccgccgg ggcggcgatc ggcttggccg ggttggccgt
     3301 ccgcggctgg gcggcggggc acatccggaa agacaaggac ctggcggtca cgggacctta
     3361 ccggttcacc cgaaatccgc tttatgtcgg aagcttcatt ctcggcctcg gcctcgtctg
     3421 ttgtgcttgg acctggtggg gagcggcgat tttcggtctc tattttctcg tgttctatgt
     3481 gccggtcatg gccgaggagc gcgaccgcct ccggcgcctt tttccccaag cctacgctga
     3541 atacgagaaa cgcgttcccc tctttttccc gacgctccgg ggcgcggccc caagcgagcc
     3601 ccggaaatgg gaggccgccc tgtttcgccg aaacaaggaa ttccgggctt ggctcgggac
     3661 ggcgatcgtc tggggactgt tcatcctccg gggatggatt cgatgacgcg gacgcccggg
     3721 cgcctggccc gtcttcgcac cgtcctcggc cgtcgccaaa cggacctccg gatcgtcctg
     3781 gaggaaatca ccaacgccca caacgcggcc gcggtcctcc gcacctgcga cgcggccgcg
     3841 atccttcatg tcgaccttat ccaggccgag ccggccccgc tgcccttcaa ccaggcgatc
     3901 accacccggg cggacaaatg gctcgtcctg catcaccatc caagccccgc ggcgtgctac
     3961 gaaacgctca aagccgccgg cttccagatc gcggccgccg acctgagccc gaaggcgatc
     4021 ccccacaccg agctggatta ctgccggccg acggcgatcg ttttcggcag cgagcgcgag
     4081 ggcctctcgc cggcggcccg agatggggct gacgtccgga tcaagatccc gatgttcggg
     4141 atggtccaga gcctcaacct ctcggtctcg gccgggatca tcctctatga agccgtccgc
     4201 cagcggctcg cccgagggtt cttcgattcg ccccgcctgt ctcccgagga attcgagaag
     4261 ctcctcgccg gctggatgga gctttaaggg gttactcgag cccctcccct cctcaaattc
     4321 gataagaacc gcggagccgc ggcggccgcg tcccttcctt ttcgaattct tcggccagcc
     4381 ttctcatctg ttcccggctc agggtcccga gcggccggtc tccgaagaga tccctcaaat
     4441 cccggaccgc cccccgaaac accgaggcgg ctccctccgg ccgtttatcc tcctcggcga
     4501 ccggaatctc gatcgtcatg ggcttgcggt cacggagaat ctccagcttg accttgtccc
     4561 ccgctttttt atcccggatc aagtcgctca agcgggcgac cgtatccatc gttcggccgt
     4621 cggccttgag aatcacgtcg ccggccttga ggcccgcttt ttgggccggg ctgtccggag
     4681 cgaactcggt cacgagcagc ccccggccgt ccttgacgcc gaagaacgcg gccagatcac
     4741 gggtcagctc ttggagagtg accccgatgt agcggcggtt ttcccagacg aagaaccgtt
     4801 ccggaccgcc gggcgcggcc ggccgttcgg ttcccttgac aagcggaggg aacagcctcg
     4861 gaaaccgggt ttccagctcg aggcggccgt cctcgggggt gaattctccc agcttgacct
     4921 tcacctcctt ggattctttg tcccggagaa tttccaggac aacctcggtc ccgggcttcc
     4981 gcttccggat ctcccgggaa aacgccgccc cggaggggat ctcggccccg gccgccttga
     5041 cgatgacgtc gccgggtttc agcttggccg cttcggccgg gctttccggc tcgacttccc
     5101 tgatttcgag acggccgtcc ttttcggcga tcataatccc aatccatccg cggaggactt
     5161 tgccgtcctt ccggatgtca ttggcgaccg agcggacgac atccgccgga acggccaggg
     5221 ccatcccgga ggaaggccct tcggcccggc tgaagaccgt tccggtgccg acgacttgct
     5281 gctcccggaa ttcgaaatag accggctggt cctcgaagaa agttccgcgc aggacgccga
     5341 tcagcctccc ctcggaattg acgaccgggc tgcccgacga gccgggcatc acccagaggt
     5401 tgagccggat gcggtccgcg gtcgccgagc tgacgatccc ttgggtcacg gccggcgtgt
     5461 cctcgggaga gaagccgacc gccccggccc agacgcccgg ccgggcgtcg gccgtcgtcc
     5521 cgagggcgat gggggccagg cccttattct tgacctcgat cacggccaag ccggtcaggg
     5581 tgtcgaatcc cttgaaatcg gctggatatt gttttccgtc gaatgtccgg acggtgattt
     5641 tttcatcccg cggcgagatc aaggcggtgg tgacgatcgt cccctcggcg tcgatcacca
     5701 cgccggtggc gatcttgcgg acgccgtttc gggcctcgac tttcacgacc gagggcgccg
     5761 tcgatttcag gatgctttcg atattccgct cgtcggcggg gacggcggcc gcgacggccg
     5821 acaacgatcc ggccgccagg acgagggcca ggagggcggc cttgaaaagg aaatgacgat
     5881 gggacatggg atcctcctta ataacggatc atcgcgaacg acgaatccga gatttgttcc
     5941 agaagataga cggcttccgc gtccgtcgag ccggcccgga tttcccggcc gtaatcgatg
     6001 gtttccatga ccgggatcgc ggacggctcg gcggccgaga cggcatatcc gccggcggtc
     6061 cgcggctcct tccccggaac gacgaaattc aagatggtga aaaaagccag gagagcggcc
     6121 gccgacccgg aaagcgccca cttgaataag cgcacccgcc gttcttgggg ccgcgcccgt
     6181 cgcctccggt ccagttcccg caacacccgg tcctcgaaat cctccggggc cgcgacccgg
     6241 gccaatcctt tccacgcctt agcatcgtcc atcgctgatc tcccgtttag cgaacgcggt
     6301 gacgccgtcc gacgccccct ccaagctttt ccggagcatg gcccgccccc ggctgatgcg
     6361 ggccttgacc gttccgaccg ggcttttgac gatcgccgcg atttcctcct ggctgtagcc
     6421 ctcgacgtcc ttgaggacga cggggatccg gtaccgcgga tgaagagcgg ccaaggccgc
     6481 ccgcaagttc cgggtcaatc ccgtgtccga ttcctcgccg gccggcgtgc tgatttcgac
     6541 ggtcgggggg agatcctcca aggaaacagc catcatccgg cggcgcctct tgtgatcggt
     6601 tttcgccagg ttcgaggcga tggcatagat ccaggaggag agcggggcga tcggccggta
     6661 gcggtcggct ttgaaataaa cccggatgaa cgtctcctgg gacaggtcga ccgccttttg
     6721 ataatcgctc gtcacctggt acaagtaatt cacgatcttg tccttgtagg cacggacgat
     6781 ctcggcgaag gccgagccgt cgccttgctg cacgcgggcg atgagcgcct gatcatccat
     6841 gctcatgatg attaaaccct cggggcgttc cggacgttgc gttcctccct ccaacataga
     6901 ctgatccgcc cggcttttca agaacgccct ccaggggccg gcggcccggc cggttgggcc
     6961 tgaaaaccgc ttccggtata atgtccagca tgaacgggac aaaggaacga tcgattctct
     7021 tttccccctt gaaggtcgga acagcggaga ttcccaaccg tttcgtccgg tcggccaccc
     7081 atgagttcct ggccggaaag gacggccgga tgacggagcg gcaccgggat ctataccggg
     7141 ccttggccga gggaggcgtc gggctgatca tcaccggtca cgcctatgtc aaccgcgcgg
     7201 gcatcgccag ccccggccag acagccgccg acgacgaccg cctcatcgag ctcttccggc
     7261 cggttcccga ggcggtccac gggacgccct ccaagatctt cctccagatc gcccacgccg
     7321 gccgccagac caaggaacgc ctgacggggg gcgtccccct cgccccctcg gccgttcccg
     7381 atcccgtctc caaggtcacg ccccgggaaa tgaccgaggg cgaaatccac ggccttgtcc
     7441 gggatttcat ccaggccgcc cggcgggccg cggccgccgg tttcgacggg gtccagatcc
     7501 acgccgccca cggatatctc ctcagcggct tcctttcgcc ccataccaac cgcaggacgg
     7561 acgcctgggg cgggtcgacc gaaaatcgtg cccgggtcgt cgtcgaaatc ctcgccggca
     7621 tccgggcgga gctcggccgg aaatttcccg tgatcatgaa aatcaacgcc tccgatttcc
     7681 tcccctccgg attggagccg gaggaagcga tcgccgtcgc ccgccagctt gagcccctcg
     7741 ggctcgacgg tgtggaggtc agcggcggaa tggccgaggc gggccgcggc tcggtctggc
     7801 cggggctcag gaccgaggac gaggagggtt atttcgtcgg ggacgccgcc cggttcaaag
     7861 ccgccttgaa gattccggtc ttcggcctgg gaggaatccg gaccttccgg gtcatggaac
     7921 gcttcgttca agaaggacgc gtggatttca tctcgctcag ccggccgctg gttcgggaac
     7981 cggacctggt ccgccgcttc cggctggggc aggccgaacg atcggcctgc ctctcctgca
     8041 acaaatgctt caatccccgc gggctctcct gcggggatct ccggaagcgg ccgtctccct
     8101 gagcgggact cccggggatc gtcccccccc ggccggatcg gcggattgac tcggccgccg
     8161 ttttaggatg aaatcgtttt gtttctttct tcagggtttc attaagctat tgatatattt
     8221 ataattcaat gacgggaacg gccgccatga tcctttttcc gatatcttcc gctctttcca
     8281 cggaccggga aaatcacgct tccggtacgg aactccgctc tcctggaatc cgtttataaa
     8341 ggtgaagtga gtggatttgg acgcgatcat acaaggttgc cgggacggca acgaaggaga
     8401 ctggaaagtg ctggttgatc ttttctcgaa aaaagtattc aacttgtctt accagtttgc
     8461 cggatcatac caggaagccc aggatctcac ccaggaaatc ttcgtcaagc tgtttcatgc
     8521 cctcccgaaa tacgagcccg gccggaattt ctcggcctgg ctgaccaccc tggccaaaaa
     8581 ccacctcatc gacaaatacc ggcggacccg gtgggaaaag aagaaccggg acgatttcga
     8641 ggaccatctt ttctcggccg cggcccccga cggcgacccc gaggcggatc ttctgcgcgg
     8701 cgaacggcgg agaatcctct gggagggcct caatcagctc cctcccgaca tccgcctggc
     8761 cgtccttctc aaggaaatcc acggcaaaag ctacggggaa acagccgaaa tcaccggcgt
     8821 ccctgaaggg acggtcaaat cccgggtcaa ccggggccgc cttcaactgg cccggattct
     8881 ccaaaaaaca agggagaaag ccgatgtcct gtaaccgcat cgaagagctc cttcccggtt
     8941 tccttgacgg cgatctcggc cccggggaac gggccatggt cgaggaccat cttcggactt
     9001 gcccggcctg cgcctcgctt ctagccctct tgggcgagac cgacgcggcc ctggcctcgt
     9061 ttcccgaagt cgaagtcggg gacgatctgc gggaacggct ggcggcgatc gccgggccga
     9121 tacggcgttt ctccctgttc gcctggctgc ggacgcccgc tttccaaccc gtcctggccg
     9181 cggtcacggc cgtcggcctc gccgtttcgc tgtatttttt gaatcccggg cggcgggatt
     9241 ttgaaaagaa ggtcgtccgg acgtttcacc gcggcgtcgg ccaagtcgag cggatctacg
     9301 cccgaacggg attcttggcc ggctccatcg aatccacggc cgaaaatctt tatgcctcgc
     9361 tccggtcgat gaaatccccg gaccgggaca aggattgaat attcttccaa ggaggcgcat
     9421 atcatggccg atcccattcc gacggaaaag cgtccgtcaa aatcccccgc cgtggccggc
     9481 attttgtcgg tggtcctgcc cggagccggc gccttgtaca acggcctgta tacgaagggc
     9541 atcctgtaca tcttgatctt cgccgggctc atcaccatgc aggaccgcgg cggccagccg
     9601 tttttcgcgc tgatgctggc cgccttctac ttcttccaga tcatcgacag cgtcaacaac
     9661 gccaaggccg tcaacaagac ggcggccggc ggcgcggcgg cttccgagga acccgttccg
     9721 gcgccggcgc cgctttcccc ctccccttcc ggctcgatct tctggggcgt cttgatgata
     9781 gccctcgggg ggattttcct cctgggcaac ttcgacgtga tcgatttcga ccggctctgg
     9841 gatttctggc cggtcatcgt catcgtgatc ggagtgaaaa tgatctccga ttacttctcg
     9901 agaaagaacg cctgagaaag gagatgttcc atggccaagc gtaaagaccc ccttatttgg
     9961 ggacttgttc tcgtcgccct cggtctcgtt ttcatcctcg aaaatttcca tgtcgacgcc
    10021 tgggatttcg tttggcggtt ctggccggtc gtcctgatcg tctggggcgc gaccaagctg
    10081 gtcgacggcc tcaagagcaa atcccggcgc gacgacgccc cgctcccccc ggcgcctccc
    10141 caggcctgag atgcgcggcc gcgaaatcgc tctcgccgtt ctcctcatcg ccctgggcgt
    10201 gctcgtgacg tatgaaaagt ccggccgcct ggacggatgg ttccagagcc cggacggccc
    10261 ctggttcggc cgcgacgagt atgtctacga ggagtcccgg gacatcgacg gccccccgcc
    10321 ggccgagatc caggtgatca acgcccacgg gcggatcgag gtgacgggga cggccgagtc
    10381 cggcccggtc cgggtcgttt tccgcaagca cgtctacgcc cggaacaaaa cggaagccga
    10441 ccgcgtcgcg gccgccctcc ggatgaaggc cgcgccctcc gggccccgct tgatcctgga
    10501 gaccaaccgg agcgaattcg agcgcaagag gttttcgacc gcgttcctcc tcgccgttcc
    10561 ggccgggacg gcggtggttc tgaagaattc gcacggcctc gttcgagcgg aggccgtcgc
    10621 ctcggccgag atctccaatc ccaacggcga cgtcgtcgtc cgggacttgg ccggccccct
    10681 cgtcttgagc tcaagctacg agagcgtcga cgtcgccggc gtcggcggca acgcccggat
    10741 cgacgcccct cactcccggg tcgtcctgaa aaagatcaag ggcgggctga tcgtgagcca
    10801 ttcctacgat aagatcgacc tggaggaaat cgacggcccg gtttccgtcg agggaacgca
    10861 ttccaaggtc acggcccgag gcctggcctc gacggccggg atctccacaa cgtacgaagc
    10921 cgtccgggtc gaggacgccg gggccgtcac gatccgggcc cgtcacagcg acattttcgc
    10981 gaaaaacatc ggcgggacgc ttgacgtgac gaacacgtac ggccgcatcc gcttggagaa
    11041 tatcgagggc gagctcaaaa tcgacggccg gaacgtcgcc gtcgaggggc ggggcgtccg
    11101 gtcgtccgat gtcttcgtcc agacgactta cgaaaacgtc ttcctggccg gcggcttggg
    11161 aaaaatttcc gttgccttga cccacggccg tctccgcctg gagcccgagg cggccttgac
    11221 cgggccggtg gatgtccggg gcgattacgc ggacgtccgc ttgctttggc cggccggatt
    11281 ccgggctccg ttccaagccc gcatccgcga cggccggatg atctggaatc tgcccgatcc
    11341 cctcgattcg gaaaaatcga acggcgtcac cgaagtccgg gcgttctccg gggcggccgg
    11401 gaatccgggg gtgacgattg aaacggccca cggcgacgtc caggtcgacg cggccgggcg
    11461 gtgaggcgcc tcagggtttc gaccgcgggc agaggatccg ggcgctcagc gcgccgttga
    11521 tcttgatgtt gaacaggttg atcaagttgg tgagatcctt cttgaacttc gtcacgtagc
    11581 ggacgaaccc gatttccatc ttgaagcgga gcagattctc gaattccccc acttcgtcgt
    11641 tgagctcgat caggtaatgg gcgatcgccc ggtactgggc gttgatctcg atgagatcga
    11701 tttcactgac cttggccgcc tccaggtggg gcggcaggga ttcgagaatg gccaggcttt
    11761 cgctgttgat ctccccgacc gattcgcgga tctcctgaaa aaacgacttg tactcgagcg
    11821 gctttttcag cttggcctcg gcccggtcga tcagtttttc gctctgggtt ttaagcgacg
    11881 cgatcagctc tcgcacgccg aaatatttcc gccggaaatt gtagagatcg atgaaatcgc
    11941 ggccgatgta gatcttatcc tcgtagtggc tcagcagctc gacgggtgaa tgcgtgtaat
    12001 acttgacctc gtatctccgt ccgcccgagt cccgctcccc cagccgggcc aggacaagga
    12061 aattgttctg ttcgatgaaa tagctgttga cctggacgag ggctgaatag acggccaggt
    12121 tttgaacgag cagctctttg agataatcca ggaggccttc ctttttcgtc accagctcgg
    12181 agacggccgg gctgggcgtg gagctgggat gggaatacga gggggtcagg agatggagga
    12241 cgggacccga tcccagctcc aagtagaggt ttttcaaaac cttctggtga agatcgtcga
    12301 ggacgaggga ggggatcggg ggaaggtcct tcaggtcgat cttgaggagc tcttcaactt
    12361 cgttcatgtc cctttacttc cgtcgcgatg ttattcgagt ttatcaccga ggaagggaaa
    12421 aagcaatgaa tccggcccgc gtttcaaggc tcccgcctat cgatcgtcca ggcttccgtc
    12481 ccgtatttcc ggcggcgcag gtcctcgatc gcggcccgtt ccgcgtcctc caaggaacgc
    12541 gcctcgaact cgactccgcc gaaggcggcc aggccctcga tgaaacgtcc ggccgcctcg
    12601 tcgaaaccga ccggccgccc cagggcttcc gacagcgagg tcatccggat cactcccgcc
    12661 ggatcctcct tcctcaggga gacggcccgg aggagctcct catccttcgc cagggggatg
    12721 gagccgtgct ggagaaagcg gttcccgatc ctcttttggg cgctgccgat gattttccgg
    12781 ccgtcgattt cgatctcgtc gcgggccgga cgggaaaagc acggcatcgt ccccttcgca
    12841 tagccggacg gcgtctcggc ggccagggac ggcttcagac cgagcgattc caagcccttg
    12901 accagggcct gggaaatcca gaggtaggag ccccccagcg tcgaggtgaa ggttccgtcg
    12961 tcggaggaga cgacggcgta agtcacctcc cggtcatgaa ggacgagctt gccgcccgtc
    13021 ggccgccgca ccacgccgag gccgttcctc cggcagaact ccaggtcgac gacccgggcc
    13081 gtcgtttggc cggctccgag ggacaccgtc ggcctctccc atcggtagaa gcggacgatc
    13141 gtggagggat catccgtcag cgagcggaag aggaattcgt cgacggccat gttccagtcc
    13201 ccggccgtcg gaacgcggtc gacgatgaga cgccactttt ttccagacat catcctttcc
    13261 gataaaaaga aagagagaga gcgggcaatg ggactcgaac ccacaacacc cggcttggga
    13321 agccggtact ctaccattga gctatgcccg cgctctctcc accggtcgaa gccggtcggg
    13381 ccacgggaat atttagcccg gatggggccg gattgtcaac cggttgtata ggtccgccgc
    13441 gcggggacat catctgactc cgattgtttt ccatgtgtct ggacctgccc cccccggagg
    13501 agtcacgaat tttatcaacg gatgaaaggc gccgaaagcg atgattcatc attgttttgc
    13561 gtaaatattt atcagggcgg atagaggacg ttgatggctg cggttattca gccccaagat
    13621 gacgctgtgg tgaacgagac gatcgacggc cgcggccgtc accatcggat ccttgaagat
    13681 cccttcccgc ttggaaaacg gaaggtccga ggcgatcaga acgccgccgg tcataaaatt
    13741 cctcgaaagc gttgacttct ccgaaggtgg tttttataat cggccgcgaa tcccttttca
    13801 aggaggccgc tcatgtcacg ggccgatgaa tctcgaaccg ccgctcccgt gatcccgccc
    13861 gacattcccc ggctgagccc gggagtcgtt ttcgagggcg aactcgcctc ttccgaggac
    13921 atcctcatcc agggagagtt caagggaacg ctccgactcc ttccccaagc gagcgtttac
    13981 atcgaccgcg gcgcccgggt cgaggcctcc gtcgccgccg gaaacgttta tgtcttcggc
    14041 acgctgatcg ggccggcccg ggcctcggcc tgcgtgttcc tctcggccga ggccgaaatg
    14101 aaaggcgaca tctcggccgc ccgcctcttc gtccaggagg gcgcccggtt ccgcggcagc
    14161 gtccagacca cggccgactg aggcgtccgt tccagcggcc tcgttccacc ctcgccgccc
    14221 ctggagtata ataatcagcc gtcatggacg ccttcgagct cgtctccgat ttcaagccca
    14281 agggcgacca gatccgggcg atcgaagacc tctcggaagg actcgaccgg gggcttctcc
    14341 accaggtcct gatgggcgtg acgggatcgg gaaagacgtt cacggtggcc aacgtcatcg
    14401 cccgggccaa ccgcccggtc ctcgtgatct cccacaataa gaccctggcc gcccagctct
    14461 tccaggagtt ccgccgcttc ttccctcgca acgccgtcga atacttcgtt tcctattacg
    14521 attactacca acccgaggcc tacctcccct cctccaacac gtatatcgcc aaggaagtca
    14581 cgatcaacga cgaaatcgac cggatgcgcc tgaacgcgac caaccgcctc ttcgagcgcc
    14641 gggacgtgat catcgtcgcc tcggtctcct gcatctatgg aatcggcgcc ccggaaacgt
    14701 actacgccat gagctttccc ctggccgccg gcgagcgcgg gccgcggaaa cggatcatcc
    14761 aggccctcgt cgacatccaa tacgagcgcc aggaggatga tttcaaacgc ggcgggttcc
    14821 gggttcgggg cgacgtcatc gacgtctttc cggcctacgc cgatttcgcc taccgcatcg
    14881 agctggacgg ggacgccgtc ggccggatca cctcgatcga cccccttctc ggccgccaac
    14941 gggaggaatt ttccaagatc atcatccacc cccggacttt tttctccacc ccccgggaca
    15001 tcctcgaggc ctcggtcgac ggcatcgaaa aggagctgaa acagcgggtg gacgatcttc
    15061 ggacccgcgg acggatcatc gaggcggaac gtctcgagga acggaccctc ttcgacttgg
    15121 acatgctccg ggaattcggg tattgcccgg ggatcgagaa ttactccctc tatctcagcc
    15181 ggaggaagcc cggccagccg ccctacaccc tcctcgatta tttcccccgg gatttcctga
    15241 cgatcatcga cgagtcgcac gtcacgatcc cccaaatcgg ggggatgtac cacggcgacc
    15301 gatcccgcaa ggaaaccctg atcgagcacg gcttccgcct gccctcggcc ctcgacaacc
    15361 ggcctctcca gttccgcgag tacgaggagc gggtcggccc cgtcctttac gtctcggcca
    15421 cgccggctcc ttacgaaatc aaggcctccc ggaaacggat catcgagcag atcatccggc
    15481 cgaccggcct gatcgatccc gagatcgaga tccgcccggt ggccggacag gttgaggatc
    15541 tccgccggga aatcgaacga agggccgccc ggaaggaacg ggtgctggtc acgaccctga
    15601 ccaagaagat ggccgaggac ctgacccgtc attatcacga cttgggactc cgggtccgct
    15661 tcctccacgc cgagatcgaa acgctcgagc gcgtcgttct cctccgggat ctccggaaag
    15721 gagtcttcga cgttctcatc ggcatcaacc tcctgcgcga aggcctggac cttcccgagg
    15781 tctccctcgt cgccgttctc gacgccgaca aggagggatt cctccggtcg acgacggccc
    15841 tgatccagac cttcggccgg gccgcccgcc acgtcgaggg ccgggcgatc ctttacgccg
    15901 acgtcctcac cgactcgatc aaggcggccg tagccgagac gaaccgccgc cgggccctcc
    15961 aggccgccta caacgcggcc aacggcatca ccccgcggtc gatccgcaag gaaatcgacg
    16021 aaggactggc ctcggcggcc gagcgcgatt atttcgatta caccaggatc gccgaggacc
    16081 gggccgtctt cctctcgccc gagcggcgca agcggaaaat cgaggagctg gagaaagcca
    16141 tgaagacggc cgcccgggat ctggatttcg aaaaagcggc cgggctgcgc gacgagatcg
    16201 cccggttgaa aaagggcgag ctggcgatct agggagcgga cgatgtcgca ccccttgaag
    16261 gaggccgccg ccgggctgcc ggacaagccc ggcgtctacc tcttcaaaaa cgcccggggc
    16321 gagccggtct acatcggcaa agcccgttcc ctggtcgacc gggtcaaatc gtatttccag
    16381 ccgaccgacg acccgaaagt ccggaacatc ctggccgaga ccgtccgcct tgatttcatc
    16441 ctgacggcct ccgagcggga agccgctttt ctcgaaaaca acttcatcca tcatctccag
    16501 ccgaagttca acctccgctt gaaggacgac aagagcttcc cctatcttcg cctcatggtt
    16561 cgggactccg tccccggcct ttttttcagc cggaaggtcg acgcctctga cggagcccgg
    16621 tacttcggcc cgttcagtcc cgcccgggag gcgggaaaaa ccctccagct gctcacgaaa
    16681 tccttccggc tgcggacctg cgccgaagct gtcttccgca cccgccggcg cccttgcctc
    16741 gaattcgaga tcgggaactg ttcggccccg tgcgtcggaa agatcagccc tgaggactac
    16801 gccgcgtccg tccgggacgc cgtcctcttt ctggagggcc ggacgcggga gctcgccgga
    16861 atcctgcgcg gccggatgga cgccgcggcc tcggcggaac gcttcgagga tgccgcccgc
    16921 tggcgggacc tgctccggac ggttgagcag atccgctccc ggccgaaagc gatttcagtc
    16981 aagctggaaa accaggacgt catcggcctg gcccaagcgg gcgcccttca ggctttttgc
    17041 gtctttctca tgagggacgg caaggtccgg gaatccgtct ctcttatccg agaacgagac
    17101 gccgggcctt ccccggaaaa agctcttcgg gatttcctcg aggatcatta ttccggacgt
    17161 ccgcttcccg accggatcct cgtgccggcc ttgcctgagg gcgcggccgc cctacgggct
    17221 cggtggaaga cggcggcgaa gcgccgcgtc ggcctcgtcg ttccccggag aggcgacagc
    17281 cggacgcttc tcgattgggc ggttaaaaac gccgaaagcc tcctccggcg ggcggacggc
    17341 gagagggccg cggtcgagga tcttcaatcc gccctcggcc ttcagtccgt ccccgtccgg
    17401 atcgacggct tcgacgtctc gcacacccaa ggcgtggaaa cgattgcctc cctggtcacc
    17461 ttccgcggcg gccgtccggc caaggagggc taccggagat accggatccg gaccgccggc
    17521 ccggccgacg acacggccgc cctggccgag gccctggaaa ggcgctacgg ccgggttctt
    17581 cgggaaaagc agatcctgcc cgacctggtc ttgatcgatg gcggcctgcc ccagctgggg
    17641 gcggctcggg ccgtcctgga ccgcttgggc ctgaacggcc ttcccgccgt ctccctggcc
    17701 aaacgcgagg aaatccttta cactcccgac cggcccggcg ggatccggct ggcgcccaca
    17761 tcgcccgccc tcaagctcgc ccagtatatc cgggatgaag cccaccggtt cgccctcgcc
    17821 ctccaccgga agcgacgggc ggatcgaagc ctgaaataag cgaaggattc cgcctcctcc
    17881 cggccccggg ccggcggcgg acaaggagac gagcatgatc ctgggaaccg gcgtcgatat
    17941 gatcgaagtc gcccgcgtcc aaaaggcaat cgaacggaac ccgcgtttcg tcgacaaggt
    18001 cttttccccc ctcgagatca cctattgcga aggcaaaaaa aaccgcttcc aacacttggc
    18061 cgcccgtttc gcggccaagg aggcgttcat caaggccctg ggccgcggcg tgccctggac
    18121 atcggtcgag gtcgtcaatc tgccctcggg gcagccgacc ctgtccgtcc gggacggaaa
    18181 ggattacgga ttcagccggg cccatgtttc cctgacccac ctggcggatc atgccatcgc
    18241 ggtcgtcgtc ctggagcggt aagtgactcc ggctcttgat ttcccgcccg cattgcttat
    18301 cataggctca agagttctaa agaggagatt tcatgaataa aacgcgagtt cgaaacatct
    18361 ggaccgggct tctggttctc gtcctggccg ggccggtcct ggccgacgaa ggcctctggc
    18421 tgttcaacat gccgccggcc gagatcttga aggcgaaata caatttcgtc ccctcgcccg
    18481 aatggctcga ccatctccgt ctgtcctcca tccggtttgg aggagcttcg ggatcgttcg
    18541 tctcgcccga cggcctcgcc ctgacgaacc accacgtcgg gcagggggcc atccagcgtc
    18601 tcagcactcc cgagcgcgac ctgatgaaga ccggcttcta cgcccggacc cgggccgagg
    18661 agctcaaggt tccgggattg gagctttccg tcctccaaag catcgaggac gtcaccgcca
    18721 ggatcaaggg cgtcgagcgt ccggagatga ccgcggccga agcggccgag gcccgcgacc
    18781 gtgaaatcgc ggcgctcgag gaggaagaga gcgaaaaaac cggccttcgc tgcgcggtcg
    18841 tcaatctctt ttccgggggg atgtaccacc tctaccggta caagatcatc accgacgtcc
    18901 gcttggtctt cgctccggat tacctgatcg ccttcttcgg aggcgaccag gacaatttca
    18961 cgtatccccg gtatgacctc gatatctgcc tcttccgcct ttatgaaaac gaccggccct
    19021 accccactcc gcattacctg aagtggaaca cttccgggca gaaggaaggc gatctggtcg
    19081 tggtctcggg ccaccccggt tccacgggcc gccttctgac ggtttcccag ctcgcctttc
    19141 tccgcgacgt cgcctatcct tggacgctgg ccaactacga gcgccgccgg gccggcttgc
    19201 agtacttcag caagcgcggc ggcgaggccg cccgcaacgc ccgcggtccg ctgttcggaa
    19261 tcgaaaacag cctcaaggcc gtgaccggat atca
//
LOCUS       DAOOXH010000044        19200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_2748_length_19200_cov_6.424706, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000044 DAOOXH010000000
VERSION     DAOOXH010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 19200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19200
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2748_length_19200_cov_6.424706"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1566
                     /locus_tag="PKV64_06620"
     CDS             100..1566
                     /locus_tag="PKV64_06620"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPN16789.1"
                     /translation="MNTEKEPAFGPQTAFEIMFRLYVVLKIAKIHDANNVNFRDQIRH
                     LMAAMEEPLRRYKRIHIQMRRNAFFINYTRVRMSYADFHINRTMLAEMIRREIGTLTF
                     SEGLDAAELSRFIAFFSGSDPHGSFTFEEFDESFTAQGFRHIQAEKAEQLDFNATRDK
                     QNRLAAQTYFLGIAHLKEISKAEEKVSSFHVTKIWIQTVFNHLTDNESFLYGLTNIKN
                     HDEYTLNHSVNVCILSMALGRRLGLSNRELMELGIGAFLHDLGKIEIPDAILNKPAAL
                     TPEEKKLMDQHARFGAAHLLRIQGDRGFSGRALSIAFEHHYKANKDNWPSYRKKDTIN
                     LFSRIVKITDYFDAITTKRVYRPRAFTREEALNIMIQNKDEEFDPLVFKVFVEMIGIF
                     PIGSLVVLDTGEIGIVTEANPHASMAQRPLVKLIADPTGRKIDGEVVDLMDVNPRTRR
                     FARTIVKTLDPEKYGIRVADYFLSQVSEEAAFNRGRPD"
     gene            complement(1544..2947)
                     /locus_tag="PKV64_06625"
     CDS             complement(1544..2947)
                     /locus_tag="PKV64_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPN16790.1"
                     /translation="MTGLERLMNRLEILYKKRTPRSFRLFRRAQNVMIQGGSHSLRLL
                     APYPFFVRRASGPEVVDIDGNVYVDYWQGHYANILGHNPVIPDDPPAGEEPEDPSLHT
                     GFEGEDQVKLAEILIRQAGWKGHKVRFTTSGTLAAMYAVMLACGRTGREEVLKIGGGW
                     HGASPLLLKGIAYDRSLGFTKVESAGVPSAFSKKIHTVRYNDSEGLIRFLRRRGEKIA
                     CFILEPYLGAGGFIPADREYLQTAREWTSRLGIVLVFDEIISGFRFAPTGVGRLAGVE
                     PDLATFGKAIGGGHAVAAVMGKTDILECLLPRAGRRTAVRFSGGTYSAHPQYMRAGLR
                     MIRHLARHGPEIYPRIAAMGERLRRGIEDAFAAEGIDARCAGRGNDVIPGSSLFMPHF
                     LRVRHEIREAGDVLDPRRADVRMKEEILRLALLTRSVHVVHGGGAVSAAHREADIDRT
                     IEAYRDVARLLNPAVLG"
     gene            3089..4384
                     /locus_tag="PKV64_06630"
     CDS             3089..4384
                     /locus_tag="PKV64_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPN16791.1"
                     /translation="MTTTKKKFKNRVCVIGLDGVPFGLLEHLARTGVMSSLGRIIDAG
                     RLHRMKASLPEISSVSWTSFMTGTNPGTHGIFGFTDFKPKSYEIRFPNFNDVKAPVFW
                     DILAEKGVRSVVINQPSTYPARPINGVLISGFVAVDLAKAVWPLELKDELEQTGYQID
                     IDTIKSRGNPGFLWQDLIKTFMGRQKALNMFWETDWDYFQLVLTGTDRLHHFFWNAGQ
                     DPRHPQHQNFLEYYRQIDRLISKIYGAFRKTAADEENIFFLSDHGFTGITQEVYLNAW
                     LAKEKYLKFSKPQPNGFEDVSAKTRAFALDPNRIYFHHAKRFPNGTIRAAEAKILREE
                     IARKLEKLEFEGKKVVRKVINTKEIYSGPYASAGPDLIVLSEPGFDMKGSIKRKEIFG
                     RTPGLQGMHTWDDAFFLAKNDYGPDLQISDVAKIIMDRF"
     gene            4384..6594
                     /locus_tag="PKV64_06635"
     CDS             4384..6594
                     /locus_tag="PKV64_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPN16792.1"
                     /translation="MFGRNPSPRPRQESSRPASRRVVRAALFAAGLLSLGLPLAAYIG
                     PGAGFAFLSSFLVLFLTFVLALLSLLVWPFRALWRLIRGQKAYRQSRVDRVVILGLDG
                     LAPELAEKWMARGKLPNMARLREEGFYGRLRTTTPAISPVAWSSFMTGAEPSKHNIFD
                     FLSRDPKTYLPDLSSARISKPKRTIRIGRYQVPLSKPVIQGLRRSIPFWKILGEKGVM
                     STVLRVPITFPPEKFNGHLLSGMCAPDLKGSQGTFSFFTSDPAKIQKAEGGVFFPVEV
                     KDGRVETFLPGPENTMLRDPAEIKLPLRITIDTAKETARIQISGKAFALKKGEFSPWI
                     GLTFKPGLGIKIRAIAKFLVTSLSPHFEMYVTPLNLDPEKPALPISHPFYYSVYLSKL
                     LGSYITLGEANDTWALNEGALDENQFLELTYANHKEWEGMLFNALRKMKRGMIAIVFE
                     TTDSISHMFWRYLDPGHPALKKGPAALGPEVIEDLFVRMDDLVGRVRAKMGPREALFI
                     MSDHGFKPFRRGVNLNTWLHQNGYLALAEGVDEGGEWFKGVDWSRTKAYALGLGGVYV
                     NQKGREAKGIVPPAEARALRKELQAKLQGLRDGETGSVSINRMYDKDEIYSGPYKDNG
                     PDLIVGYNTGYRASWEGVTGKVTGRVFEDNPKCWSGDHCIDPAVVPGILFSSLKLADR
                     EPSIMDIAPTVLELFGQERPAHMDGRSLLPKGGPAAVSPAVRTAAAPNKKDKKK"
     gene            6591..8570
                     /locus_tag="PKV64_06640"
     CDS             6591..8570
                     /locus_tag="PKV64_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPN16793.1"
                     /translation="MNRRSFLKAGAGAAAMAALGTRRELVQKVLGKTATRGKVLVLGF
                     DGLDPRLTQTWMSEGKLPTFRALAARGGFSRLGTSTPPQSPVAWSNFITGMNPGGHGI
                     FDFMHRDPKTYYPVFSASATDEAVKTVRLGKYVLPVKGGKVRNLRKGRAFWQILEDHD
                     IPATIFKIPSNYPPVPTKQRTLAGMGTPDLVGSYGIFNYYTTEPLELNQDVGGGRIHM
                     AYVIGNRVDAKLPGPVNTFLEDRPEAEIDFRVYLDPSEPVAKIALPDQELILKEKEWS
                     RWARVRFPLMPTQNVTGLVRFYLKEIRPAFKLYVSPVNLDPADPALPISTPESYAAEL
                     EKRFGPFYTKGLPADTSALDNDVLDEGEFLAQDDQVLEESRAMLEYELKRFDSGLLFY
                     YISSTDQRQHMFWRLLDEKHPMYDPALAAVYGGTIERIYRETDDILAQALAAIDKDTT
                     VIVLSDHGFNPFYRCFNLNAWLKAEGYHVFANPFKKEERGLGFPSTDWSRTRAYGLGL
                     NSLYLNQRGREAEGIVAPGAEAEALAREIAAKLEAYRDPQTGEQVILRAAVARDVYSG
                     PCVADAPDIILGFSRGYRISNSSPLGQVNREIMETNNEKWSGDHMTAAEVTPGIVLTD
                     RSIRAEAPALYDLTATILQSFGIERPADMVGKPIF"
     gene            8580..8771
                     /locus_tag="PKV64_06645"
     CDS             8580..8771
                     /locus_tag="PKV64_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16794.1"
                     /translation="MATEKVKIDADLMDKVRKLAKMSGYASPEEFVQHCLEREIAALE
                     ESDSEDEVKKKLKGLGYIG"
     gene            8771..9595
                     /locus_tag="PKV64_06650"
     CDS             8771..9595
                     /locus_tag="PKV64_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16795.1"
                     /translation="MGVLNVILEAVFDVLFAPFRSLGPWPGMIFISLATGILMLLIFK
                     KTSNQEGIKRAKNQIKARLLEIRLFKNDTSGAFRAQGAMLGANFRYMGHALRPMLVMF
                     IPVLFVLAQLNLRYGAAALQPGDQALVKVILAEGVSADESAASLQAPAGLVIETPPLR
                     IEEEGEVDWRIRAGSPGLHRLTLTVGGETVEKLVAVGEEMPAKVSPLRSRKFFDLVLY
                     PGEKPLPKGSPVSKIKIDFASRNLPLFGGSVNWLIAFFVLSLVFGFGLKGVFKVEI"
     gene            complement(9597..10733)
                     /locus_tag="PKV64_06655"
     CDS             complement(9597..10733)
                     /locus_tag="PKV64_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16796.1"
                     /translation="MGFKKTAFALILAAAVVAAASAQWRDAAARTISGRGDFRALAAM
                     IEDAYPGMPESEKSEASGILAFCAFKTGDAGSETRWIAEFFESGRSDETGFIFLDFDG
                     QAAVESYLDRWRIRYPRIREIALVAGTGDEAIIPEGTLPLAVDITAPVFFKFFEGEDV
                     LRAGAFQPGFNVLGLDANALFRETRRRTYVLEVKSGSLILRKKIILDVAVSWPEGLKP
                     KPPEPPKAGWLARALGTPDPPPPPAQPPKAREYRLSLYVGGDLLLSSRKQETAQPLTL
                     GLKPSNNPMYLKPDWNIKRNDPWANPGLNSSFSIIDAIGLVGGLLKDLLQKKKTGEPG
                     SARVRTVQDLALDFRIRDAEGRPLEMRVTMNLDTRERPVVRSGL"
     gene            complement(10723..11652)
                     /locus_tag="PKV64_06660"
     CDS             complement(10723..11652)
                     /locus_tag="PKV64_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPN16797.1"
                     /translation="MTRAAILAFSVALHAVLVYLALTARIDIKILKFNEKTTTVILVP
                     RPPIRFTASPHAGEALPAAVEPAGAAAPQPGMAPRERAGGEEGLPAGSGGGGGVSGGT
                     GAPPGGSAGEMIGGAGRGGEAGLTSGFSLVYPADAMLNLSKYAREPEDAWLQPLREAA
                     KSRGSFSRYLHPSPGSAEAAVTGSGGPGIAGGTNTLKPPPAGVVAAFVPENVKTFDLS
                     GWAGGVLNAVQKNWTLGGEAWTAEWIGRVTVTVLVMKDGLLNAVEVAASSGLSLLDAS
                     ALRAIEQSGPWPALPDGFPGSSLEIQLVFQYGL"
     gene            complement(11870..14446)
                     /locus_tag="PKV64_06665"
     CDS             complement(11870..14446)
                     /locus_tag="PKV64_06665"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HPN16798.1"
                     /translation="MRIRRWSAAALALILGAAGLQAGTERVPRFALETSGLTLSRTAR
                     PAAYFDKAGRAFAVFGTESGTFEAWAYPLKLFRGCELSFFIGSSTQPIYARDIVRHID
                     ASPEATTLTYVFQSFTVRATYITSVFEPGAVILLAVDATEPLTIVCSFQPVLQPMWPA
                     GIGGQSASWDDKLKAYVISEPTRKNHGFLGSPAASGISYTPAHMLSDAPNQFQILIER
                     PAEVRHSYLPIIMAGGKGAREDVRKIYERLAADPEAVYREAAAHFRALRAATLRIRTP
                     EPELDLAFEWAKAAYDNLFVDNPDLGLGMVAGLGMSGTGGRPGFGWFFGGDAFLNSFS
                     LVRYGAWAEVKAALLFARKWQRADGKMAHELSQAAGYLKWFEDYPYAYIHGDTTPFYL
                     AACLDYYRWSGDLDFITQGWPSILKAYAWCKSTDVDGDGLMDNAKAGLGALEFGALTG
                     ILTDIYLAGAWTRAALAMRELAGAVGDQAMEATAAADFDKARRALDDRFWNEASGQYV
                     YAFNAGGRQVDEATPWSAVPITWGLTDEERAARALQRLGSADLTTDWGVRMLSNQSAF
                     YELLNYNYGAVWPFLTGWAAAAMYQRNFVLQGYGLLRTAVRHTFDNGQGFVTELFSGD
                     LNVWPREAVAHQGFSSSGVVFPLLGGLLGLSADAPSGAFGFAPRFPADWEAVDVENIR
                     CGEAVLDIAYRRVPEGVTVQVRSRSTRPVRMTFAPVFGPGTGISGAAVNGRPVQPEIA
                     DKPWDPAVQPVLEAVLTGEDVFSIDLIPAFEILPPENPTRTGETSRGLRIIGWERAAG
                     RGLKVTLEGLAGTEYSLGLIRPELVGTVTGGAISEGRLIVRIPAGAAGAYLRHAVVLE
                     GR"
     gene            complement(14461..15522)
                     /locus_tag="PKV64_06670"
     CDS             complement(14461..15522)
                     /locus_tag="PKV64_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16799.1"
                     /translation="MTEGYSIEAFRGDYEALERMAHASWRDEYGEASFPNFYRPAFLR
                     YLIDRIPGEKRDHVVAAYRGDKIIAFFLNLPQSFSLEGKIRRGVYSCLLVTRREDYRR
                     GVALAVIRRALEISRSYDYDFSLFTLETGHRSTRMMAKVAVEGRMERIRKTGVVARIL
                     DFPRAVYSEGLKGYETAALRLIGAHRPPRRKGLLPIRKYRPSDLDGCLALLAKYQRTL
                     RLALVWDREELGWELDCPDVSQTLVYEKDGRLAGLVNFILHDHLGRSVERWAWVNHLA
                     FPGLPPEERAAFIRSYLVAVQDAGFAGTVEWKRRNYPVRTLYRNRFFPYPRGVNQMSW
                     TFNPDLSLAEIPDVYEIQI"
     gene            complement(15601..16764)
                     /locus_tag="PKV64_06675"
     CDS             complement(15601..16764)
                     /locus_tag="PKV64_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPN16800.1"
                     /translation="MRDFGFILFLVLVLSVYGFSHVYLFKRLLRALPPAGPLRFGLFL
                     FALAWALLFIGGRFLFGRHPGPAAGAVIGFGYLYLALWNVTLWLTLAYDLLRLADKRL
                     FFFPPGMRADAAAGGGIAFRYILGAAAVLCLLGAWNAARPRLRMETVEIAKPAVGIRE
                     LRIAVASDFHLSPVLRTRFLDRTLNKIKALRPDLVLLPGDIVGEDTPAADREKIAAAF
                     AGLAPPYGVFACTGNHEYFGDLEKNVETLRRGNVRVLLDEAELAAGAFYLVGRKDSAG
                     ARAGDAPREWAELTAGLDPGKPLLALSHQPVRLAEPARAGVDLLVCGHTHNGQMFPIT
                     QINWLVYEHNHGLHRKDRMFVDVTSGVGTWGPPVRLGTVPEIVLITVRFAAGS"
     gene            16992..18017
                     /locus_tag="PKV64_06680"
     CDS             16992..18017
                     /locus_tag="PKV64_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16801.1"
                     /translation="MKTKKTIALAGLVALLAGSAFAGVTRSQKAAIAFKGFGSLSTTM
                     TETLAGSRKLTDSDASFEGKGLLGGLAGKAMMPSGHTGEFIDLAAMTTAQIDHKRKTY
                     TVTPIEKWAETRKEMESAGKPDETAEEQAKSPIRIIKTDFRVTDTGETRTINGFDCRN
                     YTALWTVDWENTETGEKGTDRLETAAWTTPLTGALQAVQAEETAFAEAYMKAIGIDMR
                     DFQKDILGTQWISLLAAFDPVNGQAARMEVDPAKLAREMGKIQGYPIVIDGKYFATPK
                     IKKQQDEPSTGGGLVGKALGGLLKKKSAPEEDKAPSLSFYTEVTLLKAVDVDPASLQV
                     PAGYKQK"
     gene            complement(18037..18597)
                     /locus_tag="PKV64_06685"
     CDS             complement(18037..18597)
                     /locus_tag="PKV64_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPN16802.1"
                     /translation="MTETTPLRGLIESRRSIRRFLPGPVEREKIAACLEAARLAPSAE
                     NDQGWRFLVIDDPELKARFARKAFSGIYAPTRFAAEAPVLVLLLSKIENVGLRTGQHI
                     QGIPYYALDAGIAGEHFVLQAEELGLGTCWIGWFDVRAVRRFFKIPRRYKIVSLIAAG
                     YAARRPPHERVRKSMDEIAWWNRFGE"
     gene            18930..>19200
                     /locus_tag="PKV64_06690"
     CDS             18930..>19200
                     /locus_tag="PKV64_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16803.1"
                     /translation="MKKTVVGLLTLVLLAASFTACGKKAGIPVAGSATAEDMLTLIPK
                     NVQSVFVIDVHRGMNIPFVDKALKTGEEAEKYKEAIAKIGLDPQKD"
BASE COUNT         3801 a         6229 c         5884 g         3286 t
ORIGIN      
        1 ggaggccttg gccgccggcg tcaaagtatc gaaccaagcc gtcgccaccg cttgcgccca
       61 ggccttgaag cgtcggggcg aaaggcaccg ggaggcggaa tgaacacgga aaaagaaccg
      121 gcgttcggcc cccaaaccgc cttcgaaatc atgttccgct tgtacgtcgt cctgaagatc
      181 gcgaaaatcc atgacgccaa caacgtcaat ttccgggacc agatccggca tttgatggcg
      241 gccatggagg agcccctccg ccgatacaag cggatccaca tccagatgcg gcggaacgct
      301 tttttcatca actacacccg ggttcggatg agttacgcgg actttcacat caaccggacg
      361 atgctagccg agatgatccg gcgggaaatc ggcacgctga cgttttccga ggggctcgac
      421 gcggccgagc tgagccggtt catcgccttc ttctcgggct cggatcccca cggcagcttc
      481 accttcgagg agtttgacga atccttcacg gctcagggct tccgccacat ccaggcggaa
      541 aaagcggaac agctggattt caacgcgacc agggacaagc agaaccgcct ggccgcccag
      601 acgtatttcc tggggatcgc ccacctgaag gagatcagca aggccgagga gaaagtctcg
      661 agcttccatg tcacgaagat ctggatccag acggtcttca accacctcac cgacaacgaa
      721 tctttcctct acggtctgac caacatcaag aaccacgacg aatacaccct caaccattcg
      781 gtcaacgtct gcatcctgtc catggctttg ggccggcggc tgggtctcag caaccgggag
      841 ctgatggagc tcgggatcgg cgcgtttctt cacgacttgg ggaaaatcga aatccccgac
      901 gcgatcttga acaagccggc cgcgctgacc cccgaggaaa agaagctgat ggaccagcac
      961 gcccggttcg gcgccgccca tctcctccgc atccagggcg accgcggctt ctcgggccgg
     1021 gccctgtcca tcgccttcga gcaccattac aaggccaaca aggacaattg gccgtcttac
     1081 cggaaaaagg ataccatcaa cctcttcagc cggattgtta aaatcaccga ttatttcgac
     1141 gccatcacaa ccaagcgggt ctaccggccg cgggcgttca cccgggaaga agccctgaac
     1201 atcatgatcc aaaacaagga cgaggaattc gatccgctcg ttttcaaggt cttcgtggag
     1261 atgatcggca tcttcccgat cggctcgctg gtggtcctgg acaccggaga aatcgggatc
     1321 gtcaccgagg ccaatcccca tgcctcgatg gcccaacggc ctctggtcaa gctgatcgcc
     1381 gatccgaccg gccgaaaaat cgacggggag gttgtcgact tgatggacgt caatccccgg
     1441 acccgtcggt tcgcccggac gatcgtcaaa acgctggatc ctgaaaaata cggcatccgc
     1501 gtggccgatt attttctctc ccaggtgtcc gaggaagcgg cttttaaccg aggacggccg
     1561 gattgaggag gcgggcgacg tcccggtaag cctcgatcgt gcggtcgatg tccgcctccc
     1621 ggtgggccgc ggaaaccgcc cctcccccgt ggacgacatg gacgctccgg gtcaacagcg
     1681 ccagccggag gatctcttcc ttcatccgga cgtcggcccg ccggggatcg agcacgtccc
     1741 ccgcctcgcg gatctcatgc cggacgcgga ggaaatgggg catgaacagc gagcttccgg
     1801 gaatgacgtc attgccccgg ccggcgcagc gcgcgtcgat cccctcggcg gcgaaggcgt
     1861 cctcgattcc gcggcggagt cgctcgccca tcgccgcgat ccgcggataa atttccgggc
     1921 cgtgtcgggc caaatggcgg atcatcctca gaccggcccg catatactgg gggtgggcgg
     1981 aatacgtccc gcccgagaat cggaccgcgg tccggcggcc ggcccggggc aggagacatt
     2041 cgaggatgtc cgtctttccc atgaccgcgg cgacggcatg cccgcccccg atcgctttgc
     2101 cgaatgtcgc cagatcgggt tcgacgccgg ccaggcgccc gactccggtc ggggcgaatc
     2161 ggaatccgga aatgatctcg tcgaaaacga ggacgatccc caggcgcgat gtccattccc
     2221 gggccgtctg aagatattcc cggtcggccg ggatgaatcc tccggcgccg agataaggct
     2281 ccaggatgaa acaggcgatt ttctcccccc gacggcggag aaaacggatc aggccttcgg
     2341 agtcgttgta gcggacggtg tggatcttct tgctgaaggc ggacggaaca cccgccgact
     2401 cgactttcgt aaaacccagg gaccggtcgt aggcgatccc cttgagaaga agcggcgagg
     2461 cgccgtgcca gccgccgccg atctttaaaa cctcttcccg gccggtccgg ccgcaggcca
     2521 gcatgaccgc gtacatcgcg gccagcgttc cggacgtcgt gaaacggacc ttatggccct
     2581 tccagccggc ctgccggatg agaatctcag ccagcttgac ctggtcttcc ccctcgaagc
     2641 cggtatgcaa ggaggggtcc tccggttcct ctccagccgg aggatcgtcc ggaatcacgg
     2701 gattgtggcc gaggatgttg gcataatgcc cctgccagta atcgacatac acgttcccgt
     2761 cgatgtcgac gacctcgggg ccggaggccc ggcggacgaa aaacggatag ggcgccagga
     2821 gccgcaggga gtggcttcct ccctggatca tgacgttttg ggcccggcgg aagaggcgga
     2881 agcttcgggg ggtgcgtttt ttgtaaagga tttccagacg attcatcaac cgttcaagtc
     2941 cggtcatcgg ccgtgctcct caagggaaaa aactctacca agcccgggac gccctgtcaa
     3001 tttttttggg gggagacgtc ttttccggcc ggatttccca agaaaaccgt tggcaaaccc
     3061 gactcttatg ctataatatg ccgctccaat gaccaccacg aaaaaaaaat ttaaaaaccg
     3121 cgtctgcgtc atcggtttgg acggcgtccc cttcgggctg ttggaacacc tggcccggac
     3181 cggggtcatg tcctcgctcg gccggatcat cgatgccggc cgtctccacc ggatgaaagc
     3241 cagccttccc gaaatctcct cggtcagttg gaccagcttc atgacgggga cgaatcccgg
     3301 gacccacggc atcttcggct tcaccgactt caagcccaaa tcctacgaaa tccgtttccc
     3361 gaacttcaac gacgtcaagg cccccgtttt ctgggatatc ttggccgaaa aaggcgtccg
     3421 ctcggtcgtc atcaatcagc cctcgaccta ccccgcccgg ccgatcaacg gcgtcctgat
     3481 ctccggtttc gtggccgtgg acctggccaa ggccgtctgg cccctcgaac tcaaggacga
     3541 actggaacag acgggctacc aaatcgacat cgataccatc aagtcccggg ggaatcccgg
     3601 ctttctctgg caggatctca tcaaaacctt catgggacgg caaaaggccc tgaacatgtt
     3661 ctgggagacg gactgggatt atttccagct cgtcctcacc ggcacggacc ggctgcatca
     3721 tttcttctgg aatgccggcc aggatccccg ccatccccag caccagaatt ttcttgagta
     3781 ttaccggcag atcgaccgtt tgatctccaa aatttacggc gccttccgga aaacggcggc
     3841 cgacgaggaa aacatctttt tcctgtccga tcacggcttc accgggatca cccaggaggt
     3901 ctacctcaac gcctggctgg ccaaggaaaa atacctgaag ttctccaagc cccagccgaa
     3961 cggcttcgag gacgtatctg cgaaaacccg ggccttcgcc ctggatccga acaggattta
     4021 tttccatcac gccaagcgct ttccgaacgg aacgatccga gccgccgagg ccaagattct
     4081 ccgggaagaa atcgcccgga agctcgaaaa actcgaattc gaggggaaaa aggtcgtccg
     4141 gaaggttatc aacaccaagg agatctattc cggcccctac gcgtccgccg gtcccgatct
     4201 gatcgtcctc tccgaaccgg gattcgacat gaaaggatcg atcaagagga aagagatctt
     4261 cggccggacc ccagggctcc agggcatgca cacctgggac gacgcatttt tcctggcgaa
     4321 aaacgattac ggccccgacc ttcagatttc cgacgtggcc aaaatcatca tggatcgatt
     4381 ctgatgttcg gccggaatcc gtccccccgt ccccgtcagg aatcctcgcg gccggcttcc
     4441 cggcgggtcg tccgggcggc tcttttcgcc gccgggctcc tgagcctcgg cctgccgctg
     4501 gccgcttaca tagggccggg agccggcttt gccttcctgt cttccttcct ggtccttttt
     4561 ctgacgttcg tcctggcgct cctgtccctc ctggtctggc cgttccgggc cctttggaga
     4621 cttatccggg gacagaaagc ctaccgtcag agccgggtgg accgggtggt gatcctcggc
     4681 ctcgacgggc tggctcccga gctggccgag aaatggatgg cccggggcaa gcttccgaac
     4741 atggcccgcc tccgggagga aggattctac ggccggctcc ggacgacgac gccggcgatt
     4801 tccccggtcg cctggtcctc gttcatgacc ggggccgagc cctccaagca caacatcttc
     4861 gattttttaa gccgcgaccc gaaaacctat ctcccggacc tctcctcggc ccggatctcc
     4921 aagcccaagc ggacgatccg aatcggccgg taccaggtgc cgctgtccaa gccggtgatc
     4981 cagggactcc ggagaagtat ccccttctgg aaaatcctcg gcgagaaggg cgtcatgagc
     5041 accgtgctcc gggtgccgat cacctttcca ccggagaaat tcaacggcca cctcctctcc
     5101 gggatgtgcg cccccgacct caagggaagc caggggacgt tctccttttt cacatccgac
     5161 ccggccaaga tccagaaagc cgagggcggc gtcttcttcc ccgtcgaggt gaaggacggc
     5221 cgcgtcgaga cgtttctccc gggaccggaa aacaccatgc tccgcgaccc ggcggagatc
     5281 aagctccccc tccggatcac gatcgacacg gccaaggaaa ccgcccggat ccagatttcc
     5341 ggcaaagcgt tcgccttgaa gaaaggcgag ttctccccct ggatcgggct gacgttcaag
     5401 cccggcctgg gaatcaagat ccgggcgatc gccaagttcc tggtgacgtc tctttcgccc
     5461 catttcgaaa tgtacgtcac ccccctcaat ctggacccgg aaaagccggc cctgcccatt
     5521 tcccacccgt tctattactc ggtatatctg tcgaagctcc tgggctccta catcaccctg
     5581 ggggaggcca acgacacctg ggccctcaac gagggcgccc tggacgaaaa tcaattcctc
     5641 gagctgactt acgccaacca caaggaatgg gaggggatgc tcttcaacgc cctccgcaag
     5701 atgaagcggg ggatgatcgc catcgtcttc gagacgacgg attccatcag ccacatgttc
     5761 tggcgctatc tcgatcccgg ccacccggcc ttgaaaaaag ggccggcggc cctggggccc
     5821 gaggtcatcg aggacctgtt cgtccgcatg gacgacctgg tcggccgggt ccgggccaag
     5881 atgggcccgc gggaagcctt gttcatcatg tccgaccacg gcttcaagcc gttccgccgg
     5941 ggcgtcaatt taaacacctg gcttcaccag aacggctacc tcgccctggc cgagggagtc
     6001 gacgagggcg gagaatggtt caagggcgtc gattggagcc ggacgaaggc ttacgccctg
     6061 ggcctcggcg gcgtctatgt caatcaaaag ggtcgggaag ccaaggggat tgtccccccg
     6121 gctgaagccc gggcgctccg aaaggagctc caagccaagc tccagggcct ccgcgacggg
     6181 gagacgggga gcgtgagcat caaccggatg tacgacaagg atgagattta ttccggcccc
     6241 tacaaggaca acgggccgga cttgatcgtc ggctataaca cgggctaccg ggcctcctgg
     6301 gaaggcgtca ccgggaaagt caccggccgg gttttcgaag acaatcccaa gtgctggagc
     6361 ggcgatcact gcatcgaccc ggccgtcgtc cccgggattc ttttctcgag cctcaagctg
     6421 gccgatcgcg aaccgtccat catggacatc gcccccaccg ttcttgagct cttcggccag
     6481 gaacgacccg cccacatgga cggccggtcc cttctcccga agggcgggcc ggcggccgtt
     6541 tccccggccg tccggaccgc ggccgccccg aataaaaagg acaagaaaaa atgaaccgga
     6601 ggagcttcct caaggccggc gccggagcgg ccgccatggc cgccctagga acgcgccgcg
     6661 aactcgttca gaaggttctc ggcaagacgg cgacgcgggg aaaggtcctc gttctcggct
     6721 tcgacggcct ggatccgcgc ctgactcaaa cctggatgag cgagggcaag ctcccgacgt
     6781 ttcgagccct ggccgcccgg ggcggcttca gccgtctcgg cacgtccacc cccccgcaaa
     6841 gccccgtcgc ctggtcgaac ttcatcaccg ggatgaatcc gggcggtcac ggcatcttcg
     6901 atttcatgca ccgcgacccc aagacctatt atcccgtgtt ttcggcctcg gcgacggatg
     6961 aagccgttaa aaccgtccgg ctcggcaagt acgtcctgcc ggtcaagggc ggcaaggtcc
     7021 gcaacctgcg caagggccgg gctttctggc agattctcga ggatcacgac atcccggcga
     7081 ccattttcaa aatcccctcc aactacccgc ccgtcccgac caagcagaga acgctggccg
     7141 gcatgggcac gcccgatttg gtcggctcct acgggatctt caattactat acgaccgagc
     7201 ccttggaact gaatcaggac gtcggcgggg gccggatcca catggcctac gtgatcggca
     7261 accgggtgga cgccaagctt cccggtccgg tcaatacctt cctcgaagac cggcccgaag
     7321 ccgaaatcga cttccgggtc tacctcgatc cctcggagcc cgtggccaag atcgcccttc
     7381 ccgaccaaga actcatcctg aaggaaaagg aatggagccg ctgggctcga gtccgctttc
     7441 ccttgatgcc gacccagaac gtcaccggcc tcgtccgttt ctatctcaag gagatccggc
     7501 cggcgttcaa gctctacgtc agcccggtca acctcgaccc ggccgacccg gccctgccca
     7561 tatccactcc ggaatcatac gcggccgagc tggaaaaaag attcggcccg ttctacacga
     7621 aaggcctccc agccgacaca agcgccctgg acaacgacgt tctcgacgag ggtgaattcc
     7681 tggcccagga cgaccaggtc ctggaggaaa gccgggcgat gctggaatac gagctgaagc
     7741 ggttcgactc cggcctgctg ttctattaca tctccagcac cgaccagcgc cagcatatgt
     7801 tctggcgcct cctggacgag aagcatccga tgtacgatcc ggccctggcg gccgtgtacg
     7861 gcgggaccat cgaacgcatc taccgcgaga cggacgacat tctggcccag gctctggccg
     7921 cgatcgacaa ggacacgacc gtgatcgtcc tgtccgatca cggtttcaat cccttctacc
     7981 gctgcttcaa tctcaacgcc tggctcaagg ccgagggcta ccacgtcttc gccaatccgt
     8041 tcaagaaaga ggagcggggc ctcggcttcc cctcgaccga ctggtcgagg acccgggcct
     8101 acggcctggg tctcaacagc ctctatctca atcagcgcgg ccgggaagcg gaggggatcg
     8161 tcgccccggg ggcagaggcg gaggccctgg cccgcgagat cgcggcgaag ctggaggctt
     8221 accgcgaccc ccagaccggc gagcaagtca ttctccgcgc ggccgtcgcc cgggacgtct
     8281 attccgggcc gtgcgtcgcc gacgcgcccg acatcatcct cgggttcagc cgcggctacc
     8341 gcatatccaa cagctccccc ctcggccagg tcaaccggga aatcatggag accaacaacg
     8401 agaagtggag cggcgaccac atgaccgcgg ccgaagtcac cccgggaatc gtcctgaccg
     8461 accggtccat ccgggcggaa gctccggctc tttatgattt gacggcgacc atcctccagt
     8521 cgttcgggat cgagcgtccc gcggatatgg tcggaaaacc gattttttag gagaacccga
     8581 tggccacgga aaaagtgaag atcgacgcgg atctcatgga caaggtccgc aaactcgcca
     8641 agatgtccgg ctacgcctcg cccgaggagt tcgtccagca ctgcctggag cgggagatcg
     8701 cggccctcga ggagtccgat tccgaggacg aggtcaagaa gaagctcaaa ggtctcggat
     8761 acatcggctg atgggcgtct tgaacgtgat cctcgaggcc gtcttcgacg tcctcttcgc
     8821 tccattccgc tcgctcggcc cttggccggg gatgattttc atctccctgg ccaccgggat
     8881 cctgatgctt ctcattttca agaagacgtc caatcaggaa gggatcaagc gggccaagaa
     8941 ccagatcaag gcccggctcc tggaaatccg cttgttcaag aacgacacga gcggcgcctt
     9001 ccgggcccag ggggcgatgc tgggggccaa tttccgctat atgggccacg ccctccggcc
     9061 gatgctggtc atgttcatcc cggtcctgtt cgtcctggcc cagctcaacc tccgttacgg
     9121 cgccgccgcg ctccagccgg gcgatcaagc cctcgtcaag gtgatcttgg cggagggcgt
     9181 gagcgccgac gaatcggccg cctctcttca agcgccggcc ggcctcgtca tcgagacgcc
     9241 gccgctccgc atcgaggagg aaggcgaggt ggactggcgg atccgggccg gctccccggg
     9301 cctgcaccgc ctgacgctca ccgtcggcgg cgaaacggtg gaaaagctgg tcgccgtggg
     9361 agaggagatg ccggccaagg tctcccctct ccgctcccga aagtttttcg acctggtcct
     9421 ctaccccggc gaaaagccgc ttcccaaggg ctcccccgtt tccaagatca aaatcgactt
     9481 cgcctcgcgg aacctgcccc tgttcggggg atccgtcaac tggctgatcg cctttttcgt
     9541 cctctcgctc gtcttcggct tcggcctcaa gggcgtcttt aaagtcgaga tttgagtcag
     9601 aggccggagc ggacgacagg ccgctcccgc gtatcaagat tcatcgtgac ccgcatctcg
     9661 agggggcgcc cttcggcgtc gcggatccgg aaatccaggg ctaagtcctg taccgtccga
     9721 acgcgagccg aacccggttc gccggttttc ttcttttgaa gcagatcctt gagcagcccg
     9781 ccgacgaggc cgatcgcgtc gatgatcgag aacgagctgt tgaggccggg attcgcccac
     9841 ggatcattgc gcttgatgtt ccagtcgggc ttcagataca tgggattgtt ggaaggcttc
     9901 aatcccaagg tcagcggctg ggccgtctcc tgcttccggc tggaaagcaa aagatcgccg
     9961 ccgacgtaca gggaaaggcg gtattcccgg gctttgggcg gttgggccgg aggcgggggc
    10021 gggtcgggcg tgcccagagc ccgggccagc caaccggctt tcggaggctc cggcggcttg
    10081 ggtttcaagc cttccggcca ggagacagct acgtccagga tgatcttctt ccgtaaaatc
    10141 agggaacccg atttcacctc gaggacgtac gtccgccgcc gggtttcgcg gaagagggcg
    10201 ttggcgtcca ggccgaggac gttgaatccc ggctgaaagg cgcccgcccg gagaacgtcc
    10261 tccccctcaa agaacttgaa aaacaccggc gcggtgatat ccacggccag cggcagcgtc
    10321 ccctcgggga tgatggcctc gtcccccgtt ccggccacga gggcgatttc ccggattctc
    10381 gggtaccgaa tccgccagcg gtcaaggtag gattccacgg ccgcctggcc gtcgaaatca
    10441 agaaaaatga atcccgtctc gtcggaccgg ccgctttcga aaaattccgc gatccagcgc
    10501 gtttcggagc cggcgtcgcc ggttttaaac gcgcagaaag ccaggattcc ggaggcttcg
    10561 gatttttcag actcgggcat cccgggataa gcgtcttcga tcatcgccgc cagggcccgg
    10621 aaatcgcccc gcccggagat ggtccgggcg gccgcgtccc gccactgggc ggaggcggcg
    10681 gcgacaacgg ccgcggccag aatcagggcg aaggcggttt tcttaaagcc catattgaaa
    10741 caccagctgg atttcgaggc tcgaaccagg gaacccgtcg ggcaaggccg gccaagggcc
    10801 gctttgctcg atcgcccgaa gggccgaggc atcgagcagg ctgaggcccg acgaagccgc
    10861 gacctcgacg gcgttgagca agccgtcttt catcaccagg accgtgaccg tcacccggcc
    10921 gatccattcg gccgtccagg cttccccgcc caaggtccaa tttttctgga cggcgttgag
    10981 aacgccgccg gcccagccgg aaagatcaaa cgtcttcaca ttctcgggga cgaaggcggc
    11041 cacgacgccg gccggagggg gcttcagggt gttggttccc ccggcgatgc ccgggccgcc
    11101 cgatccggtg acggccgcct cggccgagcc gggggaagga tgaaggtagc gggaaaagga
    11161 acccctggac ttggccgcct cccgcagggg ctgaagccag gcgtcctccg gctcccgggc
    11221 gtacttggag agattgagca tcgcatcggc cgggtagaca agggaaaagc cggacgtcaa
    11281 gcccgcctct cctccccggc ccgccccgcc gatcatctct ccggccgaac ctccgggagg
    11341 ggcgcccgtc cccccggaga cgccgcctcc tccgccggat ccggccggga gcccttcctc
    11401 tccgccggcc ctctcccgcg gcgccatccc cggctgaggc gcggcggccc cggccggttc
    11461 aaccgcggcc gggagggcct cgccggcatg cggagaagcc gtgaaccgga tcgggggccg
    11521 gggcacgagg atgacggtgg tggttttttc gttgaacttg aggatcttga tgtcgatccg
    11581 cgcggtcaag gcgagataga cgagaacggc atgcagggcg acggaaaaag ccagtatggc
    11641 cgcccgcgtc aaccgtgttt tcttatcgtc gtcgatggga tgcgctcctg acggatcgga
    11701 tcaagggaat agtgtatcac actcgagcgg tcgctctctt tcggttctct tcccctcggc
    11761 cgggggaaaa gcggcgggat catgacggct tccgctcaaa catccggatc gttacgattt
    11821 ttctcaatat ctaattacta aacctcgccg gtccgggatc gttgcggggt cagcgtccct
    11881 ccaggaccac ggcgtgccgg agataggcgc cggcggctcc ggccgggatc cggacgatca
    11941 ggcggccttc ggaaatcgct ccccctgtca ccgtcccgac gagttcgggc cggatcaaac
    12001 ccagggagta ttccgtccca gccaggcctt cgagggtcac cttcaggccc cggccggccg
    12061 cccgttccca gccgatgatc cggagcccgc ggctcgtctc ccctgtccgg gtgggatttt
    12121 ccggcggcag gatttcgaag gcggggataa gatcgatcga gaaaacgtcc tcgcctgtta
    12181 aaacggcctc gaggaccggc tgaacggccg gatcccaagg tttgtccgcg atttccggct
    12241 ggaccgggcg gccgttgacc gcggccccgg agattccggt gcccggaccg aaaaccggag
    12301 cgaacgtcat ccggaccggc cgggtcgagc ggctccggac ctggaccgtg acgccttccg
    12361 gaacgcggcg gtaagcgatg tccaggacgg cttcgccgca ccggatgttc tcgacatcca
    12421 ccgcctccca gtccgcggga aatcgggggg cgaagccgaa ggcgccggac ggagcgtccg
    12481 cgctcaggcc cagcaatccc ccgagcaagg gaaagacgac gccggaggag gaaaatccct
    12541 ggtgggcgac ggcctcccgg ggccagacat tcaggtctcc cgagaaaagc tcggtcacga
    12601 atccctgccc gttatcgaag gtgtggcgga cggcagtccg cagcaggccg tatccctgga
    12661 ggacgaaatt ccgctggtac atcgcggccg cggcccagcc cgtcagaaac ggccagacgg
    12721 ccccgtaatt gtaattcaac agctcgtaaa acgcgctttg attcgagagc atccggacgc
    12781 cccaatccgt ggtcaggtcg gccgatccca ggcgttgaag agcccgggcc gccctctcct
    12841 cgtctgtcaa accccaggta atcgggaccg cgctccaagg cgtggcctca tccacctgcc
    12901 ggccgccggc attgaaggca tagacatact gtccggaagc ttcgttccag aaccggtcgt
    12961 cgagtgcccg gcgggccttg tcgaaatccg ccgcggccgt cgcttccatc gcctggtccc
    13021 ccaccgcccc ggccagctcc cgcatcgcca gggccgcccg ggtccaagct ccggccaggt
    13081 agatatccgt caaaatgccg gtcaaggccc cgaattccaa ggctcccagg ccggccttgg
    13141 cgttgtccat caggccgtcg ccgtcgacgt cggtcgattt gcaccaggcg taagccttga
    13201 ggatcgacgg ccagccttgg gtgatgaaat cgagatcgcc gctccagcgg taataatcca
    13261 agcaggcggc caggtaaaac ggggtcgtgt ccccgtggat gtaagcgtag ggataatcct
    13321 cgaaccattt caggtagccg gcggcctggg acagctcgtg ggccatcttg ccatccgccc
    13381 gctgccattt ccgggcgaat agaagggcgg ctttcacctc ggcccaggcc ccgtaccgga
    13441 ccaagctgaa ggaattcaaa aaggcgtcac cgccgaaaaa ccagccgaac ccgggccggc
    13501 cgccggttcc ggacatcccc agcccggcga ccatccccaa gcccaggtcg ggattgtcca
    13561 caaacaggtt gtcataggcg gccttggccc attcgaaggc cagatcgagc tcgggctcgg
    13621 gcgtccggat ccgaagcgtc gccgcccgga gcgcccggaa atgcgcggcc gcttcccggt
    13681 acacggcctc gggatcggcc gccagccgct cgtagatttt cctgacgtcc tcccgggcgc
    13741 ctttcccgcc ggccattatg atcgggagat aggaatgcct cacctcggcc gggcgctcga
    13801 tgaggatttg gaactggttg ggcgcatccg acagcatgtg ggccggcgtg taggagatgc
    13861 ccgaggcggc cggcgacccc aggaagccgt ggtttttccg ggtcggctcg gagatgacgt
    13921 aggccttgag tttgtcatcc caggaggcgg actgcccgcc gattccggcc ggccacatcg
    13981 gctgaagcac cggttgaaag ctgcaaacga tcgtcagcgg ctcggtcgcg tccacggcca
    14041 gaagaatgac cgctccgggt tcgaaaacag aggtaatgta cgtcgcccgg acggtgaacg
    14101 attgaaagac atacgtcagc gtcgtcgcct ccggcgaggc gtcgatgtgc cggacgatgt
    14161 cccgggcgta aatcggctgc gtgctcgaac cgatgaaaaa agacagctcg cagccgcgga
    14221 aaagcttcag cggataggcc caggcctcga acgtcccgct ctccgtcccg aaaacggcga
    14281 acgcgcggcc ggccttgtcg aaataagccg ccggacgggc cgtccggctg agcgtcaggc
    14341 cggacgtttc cagggcgaac cggggaacac gctccgttcc ggcctgaagg ccggcggccc
    14401 cgagaatcaa ggcaagcgcg gcggcgctcc aacgacggat gcgcatggaa gccctcccgt
    14461 tcagatttgg atttcgtaga cgtcggggat ctccgcgagg gagaggtccg gattgaacgt
    14521 ccagctcatc tggttgacgc cgcgcgggta gggaaagaac ctgttccggt aaagcgtccg
    14581 gaccgggtaa ttgcggcgct tccattcgac cgtcccggcg aaacccgcgt cttggacggc
    14641 cacgagatag gatcggatga aggccgcccg ctcctccggc ggaagcccgg gaaaagccag
    14701 gtggttcacc caagcccagc gctcaacgga ccggccgaga tgatcgtgga ggatgaagtt
    14761 gacgaggccg gcgaggcggc cgtctttttc ataaaccaag gtctgggaca catccgggca
    14821 atccagctcc cagccgagct cctcccggtc ccagaccagg gccaggcgca gggtccgctg
    14881 gtatttggcg aggagggcca ggcatccgtc caggtcggac ggccgatatt ttcgaatcgg
    14941 cagcagcccc ttccgccgcg gcggccggtg ggcgccgatc agccgcaagg ccgcggtttc
    15001 ataccctttc aagccttcgg aatagacggc ccggggaaag tcaagaatcc gggcgacgac
    15061 cccggttttc ctaatccgct ccatccggcc ttcgacggcg accttggcca tcatccgggt
    15121 cgaacgatgc cccgtctcga gggtgaagag agagaaatca tagtcgtagc tccggctgat
    15181 ttcgagggcc cggcggatga cggccagggc cacgccgcgg cggtaatctt cccttcgagt
    15241 gaccaaaagg cagctgtaga cgccccggcg gatctttcct tcaagagaga agctctgcgg
    15301 caggttcaga aaaaaggcga tgatcttgtc gccccggtac gcggcgacga catgatccct
    15361 tttttccccg ggaatccggt cgatcaggta gcggaggaac gccggccggt aaaagttcgg
    15421 aaaggacgct tccccgtact catcccgcca ggaagcatgg gccattcgtt cgagagcctc
    15481 atagtcgccc cggaacgcct cgatggaata tccttcggtc atgagatttc cttttccgaa
    15541 ttatttatca caagatcccg ggagggacaa gaaagaacga tcggagggga agacggcggc
    15601 tcaggagccg gcggcgaagc ggacggtgat caggacgatt tccgggacgg tgccgagccg
    15661 gacgggcggt ccccaagttc caacgccgct cgtgacgtcg acgaacatcc ggtccttccg
    15721 atgaaggccg tgattgtgct cgtagacgag ccaattgatc tgggtgatcg ggaacatctg
    15781 gccgttgtgg gtgtggccgc agacgaggag gtcgacgccg gctcgggcgg gttcggctaa
    15841 tctcaccggc tggtggctga gagcaagcag aggctttccg ggatcgaggc ccgcggtcag
    15901 ctccgcccat tcccgggggg cgtcgcccgc ccgcgctccg gcggaatcct tgcggccgac
    15961 gagataaaac gccccggccg caagttccgc ctcgtcgaga aggacgcgga cgttcccccg
    16021 gcggagcgtt tcgacgtttt tctccaggtc cccgaaatat tcgtggttcc cggtacaggc
    16081 gaaaacgccg tatggcggag cgagtccggc gaaagcggcc gcgatcttct cccggtcggc
    16141 ggccggagta tcctctccga cgatatcgcc ggggagcaag accaggtcgg gccggagggc
    16201 cttgatttta ttgagggttc ggtcgaggaa ccgcgtccgc agaaccgggc tgaggtggaa
    16261 atcggaggcg acggcgatcc tcagctcccg gatcccgacg gccggcttgg cgatctcgac
    16321 cgtctccatc cggaggcgcg ggcgggcggc gttccaagcc cccagaaggc agaggacggc
    16381 cgccgccccg agaatgtacc ggaaggcgat tcctccgccg gccgcggcgt cggcccgcat
    16441 ccccgggggg aaaaagaaaa ggcgcttgtc ggccaggcgg aggaggtcat aagccaaggt
    16501 gagccacagg gtgacgttcc aaagggccag gtagagatag ccgaagccga tgaccgctcc
    16561 ggccgccggc ccgggatgtc gtccgaaaag gaaccggccg ccgatgaaga gcagggccca
    16621 cgccagggcg aacaggaaaa gaccgaagcg gagaggcccg gccggcggca aagcccggag
    16681 aaggcgcttg aacaaataga cgtgggaaaa accgtacacg gacaggacga ggacgaggaa
    16741 gaggataaat ccaaaatcgc gcatcgtctc ggccgtggaa agggattact atacctgaac
    16801 ggccggggcc tgccaagcgc caaagccggc ccgaaggatg gggaacccgt ttccctcgtt
    16861 ccgggatctc cccgaagccc gggcgggccg tctcccccct ccgcctggac cgggacagcg
    16921 tcctcggggg cctgcggatt caggcctccg cgtttaaaat gttttttata agatttccca
    16981 ggaggtctcc catgaagacg aaaaaaacca tcgccctcgc cggcctcgtc gccctgctgg
    17041 ctggatccgc cttcgccggc gtgacccggt cgcaaaaagc cgctatcgcc ttcaagggct
    17101 tcggcagcct gtccacgacc atgactgaaa ccctggccgg aagccggaag ctgaccgaca
    17161 gcgacgcctc attcgagggc aagggcttgc tgggaggctt ggccgggaag gccatgatgc
    17221 cttcgggcca caccggagaa ttcatcgacc tggcggcgat gacgaccgcc caaatcgatc
    17281 acaagaggaa gacgtatacc gttacgccca tagagaaatg ggccgaaacc cgcaaggaga
    17341 tggagagcgc cggcaagccg gacgagacgg ccgaggaaca ggcgaaatcg cccatccgga
    17401 tcatcaagac cgatttccgg gtcaccgaca ccggggaaac ccgcacgatc aacggtttcg
    17461 actgccggaa ttacacggcg ctttggacgg tggactggga aaacaccgag accggagaaa
    17521 aaggcaccga ccggctggaa accgcggcct ggacgactcc gctgacgggc gcccttcaag
    17581 cggtccaggc cgaggaaacg gccttcgccg aggcgtacat gaaagccatc gggatcgaca
    17641 tgagggactt ccagaaggat atcctcggaa cccaatggat cagcctgctg gcggcctttg
    17701 atcccgtcaa cggccaggcg gcccggatgg aagtggatcc cgccaagctg gcccgggaaa
    17761 tgggcaagat ccaaggctat cccatcgtca tcgacggcaa gtatttcgcg acccccaaga
    17821 tcaaaaaaca gcaggacgag ccctcgacgg gcggcggcct cgtcggaaag gcgctcggcg
    17881 gactgctgaa gaagaaatcc gccccggagg aagacaaagc cccctccctt tccttttaca
    17941 cggaagtcac gttattgaaa gccgtcgacg tcgatccggc ctcgcttcaa gtcccggccg
    18001 gctacaagca gaaataacga gaatccgggc cggccgtcac tcgccgaacc ggttccacca
    18061 agcgatttca tccatcgatt tgcggacccg ttcgtgcggc ggccggcggg cggcatagcc
    18121 cgcggcgatc agggaaacga ttttataacg ccgcgggatc ttgaaaaagc gccgaaccgc
    18181 ccggacatcg aaccagccga tccagcacgt ccccagcccc agctcctcgg cttgaaggac
    18241 gaagtgctcg ccggcgatgc ccgcatccag ggcgtaatac gggatcccct ggatgtgctg
    18301 acccgtccga agacccacgt tctcgatttt ggacaggaga agaacgagaa cgggagcctc
    18361 ggccgcaaac cgcgtcgggg cgtagatccc tgaaaaggct ttccgggcga agcgcgcctt
    18421 gagctccgga tcgtcgataa cgaggaatcg ccagccctgg tcgttttcgg ccgagggggc
    18481 cagccgggcc gcctcgaggc aggccgcgat tttttcccgc tcgaccggac ccggcagaaa
    18541 ccggcggatg ctccggcggc tctcgatcag ccctctgagc ggcgtcgttt cggtcatctc
    18601 ggttcctcct ccacggccgc ggccgggcgg gattcatatc ttgatatggt catcaacggc
    18661 gtattcatga tgaatttaca ccctttgggc aagccgatgg gacccgtcgg ctccgcggcg
    18721 caaggccctc ggcggggcgg ccggcgcggt ttaacatccc gctcccgcgg cccttccggc
    18781 tttgaattca cgccgtttat atcccagccc ccggccgaat tcaagggccg ggtccgttcc
    18841 ggcccccgtt tacatggccc cccccgatgt gttattcata cgttgaactc cgccggggcg
    18901 gatttcgtat ggaaaggagg aggtgttcca tgaaaaaaac cgttgtcggt ctgttgactc
    18961 tcgttctctt ggccgcatcc ttcaccgctt gcgggaagaa ggccggaatc cccgtcgccg
    19021 gctcggccac ggccgaggat atgctgacgc ttatccccaa aaacgtccaa tccgttttcg
    19081 tgatcgacgt tcaccggggg atgaacatcc ccttcgtcga caaagccttg aaaacgggcg
    19141 aagaggcgga aaaatacaag gaagccatcg ctaaaatcgg cctggaccct caaaaagacg
//
LOCUS       DAOOXH010000045        18792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_2834_length_18792_cov_9.085126, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000045 DAOOXH010000000
VERSION     DAOOXH010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 18792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18792
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2834_length_18792_cov_9.085126"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PKV64_06695"
     CDS             complement(<1..816)
                     /locus_tag="PKV64_06695"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPN16804.1"
                     /translation="MSAPKPFKLFPQRPDRTRYRRVSDRFVRRVKCSGGEALAVDHAG
                     LVLLAAEAFSDLAFYLRPAFLKRIRAIVDDPRASDNERFVAGAILRNAAIAGEGVLPL
                     CQDTGSAQVVGFKGHRVLTDGRDEEALTSGLRDVYRKRNLRYSLVAPLSVFEEANTGT
                     NLPAQVDLLASEGEEYHFYFMAKGGGSSNKTALFQESKALLEEKKLEAFLAEHVRALG
                     VAACPPYRLAVVLGGLSPEMTMKTVKLASAGFLDSLPTRGKKSGIAFRDAEWEG"
     gene            complement(827..1789)
                     /locus_tag="PKV64_06700"
     CDS             complement(827..1789)
                     /locus_tag="PKV64_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine-tRNA synthetase second additional
                     domain-containing protein"
                     /protein_id="HPN16805.1"
                     /translation="MMKSSPQRTVHRETLIYATYYAPRGRNRLYLVAAELSQKYLTPT
                     DLVIGVLGAEGSGKSTLIKGLFPGLELTNDDDGINVRPTPLIDFDPKDAFSGHTFHID
                     VRYELAFHQKFELAEVIHKAVTHGRRVIIEHFDLVYQALGYNAQVLFGIGEEIIVARP
                     TVFGPFPQAIKDIVDRTIKYRLMAHSAEDITCSILKRDYHFPFPPLHSDVRQGFVLNF
                     NHRPNVDLKELERKVNEVIARNEPIAACGENRIRIGRHAMPCTGTRTHVKTAGEIENF
                     RLLHEYRFDSRTKEYYIVGLVGQKSSAGFEEMIDIEEYLEGYGE"
     gene            complement(1807..2118)
                     /locus_tag="PKV64_06705"
     CDS             complement(1807..2118)
                     /locus_tag="PKV64_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HPN16806.1"
                     /translation="MKRSILSVAKVIRSKNSGPYELTLDIIFKDRKGYDLFRRSNAVT
                     KATIARLYRCRPADVLKIVYFEPAKALKVTLKRPIPSGHPGETDIYGAQQHAPLLSLM
                     F"
     gene            complement(2135..3499)
                     /locus_tag="PKV64_06710"
     CDS             complement(2135..3499)
                     /locus_tag="PKV64_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HPN16807.1"
                     /translation="MTSREKEFRVLSATAILGYGFPEKSFRAGLARKPHLIGADAGST
                     DPGPYYLGAGKAFTNRSGVKRDLRFMLREGVRRGIPVVIGTAGGSGARPHVDWCEAII
                     REIAREEKLTFTLGIIYADIPKERIRKHLRKGEIVPLAYVPPLTEKMLDDSLHIVAQM
                     GVEPIQEALRRGCQVVLAGRCYDPAVFAALPVMRGFDEGLALHMGKILECAAIAATPG
                     SGADCALGVLRGDSFILETLNPARTFTPESTAAHTLYEKTDPYHLPGPGGELDLTACT
                     FTALPGGRVEVRGSRHVPTPEYYVKLEGVRRTGFRTISVAGTRDPIMIKEIDAILEAV
                     TGQVRDILKAEKIDGRIQFHVYGKDGVMGPLEPETKIRSHELGIVIEAVGSTPEAADS
                     LCSITRSTLLHYGYPGRISTAGNLAFPFSPSDVRMGETFEFSVYHLMPLTGRTPFPVK
                     IVTI"
     gene            complement(3502..4752)
                     /locus_tag="PKV64_06715"
     CDS             complement(3502..4752)
                     /locus_tag="PKV64_06715"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HPN16808.1"
                     /translation="MKIVKTLFVPGSSAFYFDDQRAIKAGAGHDGFFYTGQPATPHFK
                     SVRVAGEILSILLVLENGAVAGGDCAAVQYSGAGGRDPLFLAADYLPLLETRIRPLLE
                     GREIGPFRTMAGEVENLEFDGVRLHTAVRYGLSQALLQARAFDRHRLMAEIICEEYNL
                     PVVAERVPVFGQTGDDRYVNADKMILKGVDVLPHALINNVDEKLGRNGELLRAYIRWL
                     ADRIRAFRTEPSYRPTLHIDVYGTIGQIFGLDPGRVADYLASLEEDSAEFPLYIEGPV
                     DAEEKARQIELLGKVRETLRRRGSSVKIVADEWCNTLEDVRDFTDAACCDMVQIKTPD
                     LGGIQNTIESVLYCRRHGMEAYQGGTCNESDVSARACVHAAVAARAERLLAKPGMGFD
                     EGFMIVRNEMERILAVLRARPEGK"
     gene            complement(4749..6197)
                     /locus_tag="PKV64_06720"
     CDS             complement(4749..6197)
                     /locus_tag="PKV64_06720"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005886455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HPN16809.1"
                     /translation="MRYEPMAWKDFLTQRKSVLATWPTGKDVDLEGGLRYQRDIPEAK
                     NFARAMSGAKAKGRTLIQPRAGVALIDDHIRLLRFLETEGTADLLPTTIDAYTRQNRY
                     EEAAVGIEKSTAAGHSLLNGFPAVNHGLGGCRRVTEAVTKPIQVRHGTPDARLLAEIS
                     LAGGFTSFEGGGISYNIPYAKKVELGDSLRAWRYIDRLVGWYEENGVSINREPFGPLT
                     GTLLPPFIGHTVAILEGLLALEQGAKSITLGYGQGGNVLQDLAALSSLRELAGEYFEK
                     AGFTDFDLTIVFHQWMGGFPEDEAKAFAVISLGSFVAAAAGVEKIIVKSPHEAAGIPT
                     KEANAAGLKTTRQILSMLTDQIICGDNDIIRREKNLIREEVACLMNAVENASGGDPIQ
                     GIVRAFAGGLLDVPFAPSIFNRGRVLPARDNEGFIRLFAKGNLPLSDEIMAFHKERLA
                     ERAKAEGRAVTFQMVTDDIYAISKSKLIGRPK"
     gene            complement(6202..7596)
                     /locus_tag="PKV64_06725"
     CDS             complement(6202..7596)
                     /locus_tag="PKV64_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HPN16810.1"
                     /translation="MTPSALASIDIGSTYTKGALFALEAGAFRLLSRAETPTTADNLA
                     VGFGRVREKLGPGGRGPEEFPVYLSSSARGGLKIAALGLTADLTLSIARMAAYSAGGK
                     IVRTYSYELAADEIAGLEALQPDIILFTGGTDGGNSAVVLRNAELLGRSSTAAFILYA
                     GNAKVRDRVLALLDGKAAAATDNVMPEVGTIQIEPAREVIRKIFLKRIVDGKGLSTVV
                     RETGRDPWPTPLAVFELVRRIGASQPGWSDFGLVDMGGATTDFYSHTEAFTGGDSVLL
                     RGLHEPLLKRTVEGDLGLRVSAAALWESERAYLTSRLAAAGRSIEDFDAYIHRVSAQP
                     ESRPEGGAERAYDEMMAAACVRAAALRHAGTLSRVYTPQGGFHVQRGKDLRRITRLIG
                     TGGYLSRTENAAILLEACAPGQADADSQPLLPVSPAAYADEGYLFPLLANLAGDYPDA
                     AARTAVGGLRVLKG"
     gene            complement(7593..8015)
                     /gene="glmS"
                     /locus_tag="PKV64_06730"
     CDS             complement(7593..8015)
                     /gene="glmS"
                     /locus_tag="PKV64_06730"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HPN16811.1"
                     /translation="MNPQNIVLGVIGADCHSVGNKILDAYFSEAGFHVTNLGVMVSQE
                     EFIDAAVETDAAAILVSSLYGHGEIDCEGLRAKCIERGLDRILLYVGGNLVVGKTPYE
                     EVEKKFLDMGFNRVFRPQVDLAEVVKMLKADIAAASRP"
     gene            complement(8015..9019)
                     /locus_tag="PKV64_06735"
     CDS             complement(8015..9019)
                     /locus_tag="PKV64_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPN16812.1"
                     /translation="MEKMKLDKSSSAPPAYQQIISFFDQQIKNRVLLPGDKLPPEREL
                     AEELGTARGTVKRAYEELVRRGLIEAFSGRGSFVAGEAVAPVEDRKDRAIRLIDELLD
                     QLGRLKFSAQDIRMFFDLRLLGREDSLRNLSVAAIDCNPETLTIFERQFLLIPNVNLA
                     RIDLDDLFEDPGAGARLEIFDLVLTTTTHYQELIDRFPEIRSKAVQVAVAPTRTSIIS
                     LARLTPAQRIGILSESRKFQAIILDHLRTFDIPSTGVKTLFISQLERLDEFIAALDAV
                     IVPAGYSLQKNREHLPALQAFTERGGIIIPFDYQIERGSLLHVEERLRTLLDEEKRSV
                     "
     gene            9137..13753
                     /locus_tag="PKV64_06740"
     CDS             9137..13753
                     /locus_tag="PKV64_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPN16813.1"
                     /translation="MKKGGSAAGRYRSSPWHTLQFESPTPLRAREILEAAESAVSSGR
                     LPAAASAKEYLNLTSSRTFLQALGEPDLLNRWAETVFAFIRLTGYNLLDLFESRIREQ
                     PHKILFQDMSASTPVQWTYEQVGRRVRELAGSFYAMGQEDGAEPRVAIYAENNVECAS
                     ADLACLLYDILDTPINPHFNIDNLVEIFDALNITIALTDRPDRRKILGEVRRRTARPF
                     RILTVAPAGKPENEKDGVLIEYAKRRPVRSLESLLEARRRRPVNEVATVMFTSGSTGR
                     PKGVSFSSYHLVSKRFARAAALPDVGDDEVFLSFLPLFHTFGRYLELLGMIFWRGTYV
                     FAGNPSADTLFTLFPKVNPTGFISVPVRWAQLYERCLEKLDETSGADRKSVIRSVVGQ
                     RLRWGLSAAGYLDPRVFRFFEQEGINLSSGFGMTEGTGGITMTPPGRYVDNTHGLPLP
                     GVRLRLGRDSELEASGHYIAPYLEAAAPESLIPFPENPETDYWLQTGDIFRRLSNGYY
                     QIIDRIKDIYKNNRGQTIAPRKVEDKFIGVPGIKRTFLVGDGRPTNVLFIVPDRSDTV
                     LASALVQDNGREYYRRIVTAANADLAPYERVINFAVLDRDFDAAWGEVTPKGSFNRKR
                     IEENFSGRIEELYRKPYLELAGPGGTVRIPLWFFRDLGILEDEITRDARGLRDRNRKL
                     RLPLRPTARPKVWLVGDLEYEIQGTVVDLGLFARQPILWAGNPALIRFSPCKEGWDLP
                     LEPATDRVFLPDRRVRLAPTEDIAQPSNIRNTPLLRLNEILVRILFSGRKPLDGLLDE
                     LEKLFQEPDLRTTNLIRSRLRALAGHPAEDVRCWAYRLLLLDNPNVDYSRLLPAFVQS
                     GKPFLNARSIEGIVSARPGKSRLDSLRRRMIAYRETMTWPANPVTRLQFDRILRLLAN
                     LTRVHPEFFGSIRAELAAWALHTADPGLAASAEKLLAKLTHGIEKDGKKAAFGVLRKE
                     DRLVFEEGLGPAEIRRLKTLLLGTTFLHESIRFAFGPDDFNPRDIPAAGIWISRAHSV
                     SSFPDYRISLNTTSSRHYDLKLFLKGRPWSDREWRTILWSAFLADHPEETAAMAPLGA
                     IRLGEGAVSRRYIGDLTLWEKIREYAGRRAAGAPPPELAVWRKLYIEAMSVFFRAWKM
                     TGRRIVPGPVSPLNAIVPEIEYRDATIIASLDSRTPYRNTLSIVRPLVDRFYRRTAAD
                     YPWIREQTDLEWIFDACYNALGRDEAAAFFVELQNDLGGGKPASPELAGLRRLLAEYR
                     ARFEKSYRIPLAGLNAVRAFQEWEAANPKADSRDRERKVLDLHRKYDLGRFPEIVRYH
                     LYRHTYFAGRSLKEEELFDRLLALMSENPKAPAVRHVELSDLQAALNDERDRAVFSRM
                     IFPHLEPDRRLDVVVEGEEGAERIVVRSFLVDRRGETYAFSETTDPADIGRLYRLFYK
                     ENFPKVISQQDRHFILQDRQNRIVGGLCYRVMSRSVVFIDAVVITSRLKSSGLGGAVV
                     DDFCGRMAGQGYTTVLTHFYLPGFFLRRGFRLDKKWGALAKSIG"
     gene            complement(13750..14163)
                     /gene="rnk"
                     /locus_tag="PKV64_06745"
     CDS             complement(13750..14163)
                     /gene="rnk"
                     /locus_tag="PKV64_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HPN16814.1"
                     /translation="MAKTITLTQTDYDRLKALTSVDPDPSDLDKNAFQELRAELDRAV
                     VVDPKEIDPNVVTVNSRVLIQDVATGEEKEYTLVFPLAADFSVGKISVLVPLGTALLG
                     FRKGDVVEWKVPGGLRKFKIKKVLYQPEAAGNFES"
     gene            14282..14839
                     /locus_tag="PKV64_06750"
     CDS             14282..14839
                     /locus_tag="PKV64_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPN16815.1"
                     /translation="MKSKRAKSRPPALQGQVQIYTGDGKGKTTAALGLALRAAGRGYR
                     TYFGQFMKARSSGERAAARRLGGLITFATFGRPGLIRTAGRPDPEDVRRARRGLDLCR
                     KAMLSGRYDIVVLDEINVALHFGLLGLPDVLALLDEKPPSVELVLTGRRAPEALIRRA
                     GLVTEMKEIKHYFQAGVPARHGIED"
     gene            15029..16027
                     /gene="pta"
                     /locus_tag="PKV64_06755"
     CDS             15029..16027
                     /gene="pta"
                     /locus_tag="PKV64_06755"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HPN16816.1"
                     /translation="MTLLESLKEKAKATPRRIVLPEGEEPRTIKAAAQIAKEQLAQVT
                     LVGNRAKIEAVAKEQGVDVSGIPSVDPAASPKFEEYVQAYYEIRKAKGMTPEEARKLM
                     SDPVYFGTMMLHKGEADGLVSGVVHSTGETIRPALQIIRTKPGINIVSSSFVMIVPDC
                     SYGDNGMFVFADCAVMPAPSDVELAQIAVASAETARVLCGMDPKVAMLSFSTYGSAKH
                     ELVDKVKKAVAVAKELAPGLKIDGEMQADAAIIPKIGKAKAPNSAIAGQANVLVFPDL
                     QSGNIAYKLVERLAKAQAVGPILQGIAKPVNDLSRGCSVDDIVHLTAITAVQAAGL"
     gene            16069..17409
                     /locus_tag="PKV64_06760"
     CDS             16069..17409
                     /locus_tag="PKV64_06760"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPN16817.1"
                     /translation="MIVLSLNCGSSSAKYRLYDYDKKVTLATGIVERVGIGKSFIQQA
                     GLGRAPIQIDHDCANHKDALKLIIDTLLDPKVGVLDDLKKISAVGHRVVHGGEKFKKS
                     VIIDAASLDTFKALYSLAPLHNPPNVLGIEAAMDVLPSIPHMAIMDTAWHQTMPAHAY
                     MYALPYEWYEKYGVRRYGFHGTSFLYVAKRAALLLGKDPFKTNLICLHIGNGVSANAI
                     KNGVSYDTSMGLTPLEGLVMGTRAGDHDAAIDLMMMEKENIAPAKMNDLLNKKSGLLG
                     ITGKYTDRRDVQKAAEAGEARAALAIEMESYRIKKYIGAYLAAIGGADAIVFTAGVGE
                     MSDLIREKSLDGLEFLGIKLDKAKNKLAKTRNAETEISAEDSKVKIFVIPTDEERVFI
                     EDVIALLQGTYDEHTRFTYRFQHPDYVNSQREQDFAADCAKKPGLDKIQVKPKK"
     gene            17583..18617
                     /locus_tag="PKV64_06765"
     CDS             17583..18617
                     /locus_tag="PKV64_06765"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPN16818.1"
                     /translation="MKAAFLTAPRKVEIRDVPKPLLKNDDDVLLRTALAGLCGSDLHY
                     FTADRIGGEAVPYPAVVGHECSAFVEAVGPSVKQFKAGDRVAVEPSISCGTCDQCRAG
                     RPHTCRHVSFLGHPGERSGCLAEYFVMPERNLLSVPETLTMTEAMLVEPLSIALHARA
                     LAVGTLGPSIGVLGTGPIGLLLVQVLKTEGHGDIFATDRSDARIAAALKAGAAWSGNP
                     DRLDVVSEIFKRKPLGLDAVFEASGDPAAIEQAVELVKPGGWIYQIGIPPDERISYLS
                     ARLRRKEIGLRFLRRQNRCVNQGLMLVKAKHLDIDWLATHTFKIEEAEKAFATAADRT
                     DGVLKSSIVF"
BASE COUNT         3480 a         6030 c         5873 g         3409 t
ORIGIN      
        1 tccctcccat tccgcgtcgc ggaaggcgat tccgcttttc tttccccgcg tcggcaggga
       61 atccaggaat ccggccgagg ccagcttgac cgttttcatc gtcatctcgg gcgagagtcc
      121 ccctagaaca acggccagcc ggtagggcgg gcaggcggcc acgccaaggg cccgaacgtg
      181 ctcggctagg aaagcctcga gcttcttttc ctccaggagg gccttggact cctggaacaa
      241 ggccgtcttg ttggacgatc cgccgccctt agccatgaaa tagaaatggt attcctcccc
      301 ctcggacgcc aggaggtcga cctgggccgg aagattcgtc cccgtgttgg cttcctcgaa
      361 aacggagagc ggggcgacga gcgaataccg gaggttccgc ttccggtaaa cgtcccggag
      421 gccggacgtc agggcttctt catcccggcc gtcggtcaga acacggtgtc ccttgaagcc
      481 gaccacctgg gccgagccgg tatcctggca caggggcaga acgccctcgc ccgcgatggc
      541 cgcgttcctg agaatcgccc cggcgacgaa gcgctcgttg tccgaggccc gcgggtcgtc
      601 gacgatcgcc cggatgcgct tcaagaaggc cggccgcagg taaaaggcca ggtcggaaaa
      661 agcttccgcg gccagcagaa ccaagcccgc atgatcgacg gcgagcgcct cccccccgga
      721 gcacttcacc cggcggacaa accggtccga aacccgccgg tatcgcgtcc ggtccggccg
      781 ctgcgggaaa agcttgaaag gcttgggggc gctcattctc gtctccttat tctccatatc
      841 cttcgagata ctcctcgata tcgatcattt cctcgaaccc ggccgaggat ttctgcccca
      901 ccaggccgac gatgtagtac tccttggtcc gggaatcgaa acggtattcg tgcagaagcc
      961 ggaagttttc gatctctccg gctgttttca catgagtccg ggtcccggtg cagggcatgg
     1021 cgtgccggcc gatgcggatg cggttttcgc cgcaggcggc gatcggctcg ttccgggcga
     1081 tgacctcgtt gaccttgcgc tcgagctcct tgaggtcgac gttgggccgg tggttgaaat
     1141 tgaggacgaa tccctgccgg acgtcggagt ggagcggagg gaacgggaaa tggtaatccc
     1201 gtttcaagat cgaacaggtg atgtcttcgg ccgagtgggc catcagccgg tacttgatcg
     1261 tccggtcgac gatgtccttg atcgcctggg gaaacggacc gaaaaccgtc ggccgggcga
     1321 cgatgatctc ctcgccgatg ccgaacagaa cctgggcgtt gtagccgagg gcttggtaaa
     1381 ccaggtcgaa atgctcgatg atcacccgcc ggccgtgggt aacggccttg tggatgacct
     1441 cggccagctc gaacttctgg tggaaagcca gctcgtagcg gacgtcgatg tgaaacgtgt
     1501 gcccggaaaa ggcgtccttg ggatcgaagt cgatgagcgg cgtcggccgg acgttgatcc
     1561 cgtcatcgtc gttggtcagc tccaatccgg ggaagagccc cttgatgagg gtcgatttcc
     1621 cggagccctc cgcccccagg acgccgatca cgagatccgt cggggtgagg tatttctggg
     1681 acagctcggc cgcgaccagg taaaggcggt tccggccgcg cggggcgtaa tacgtggcgt
     1741 agatgagcgt ttcccggtga accgtccgct gcggactgga cttcatcatg tccggcctcc
     1801 tccgcgtcag aacatcaggc tcaagagcgg cgcgtgctgc tgggcgccgt agatgtcggt
     1861 ctcgcccggg tggccggacg gaatcggccg cttgagggtg accttcaggg ccttggccgg
     1921 ctcgaagtaa acgatcttga ggacgtcggc gggccggcag cggtacagtc gggcgattgt
     1981 ggccttggtc acggcgttcg accgccggaa caggtcatag cctttccggt ccttgaaaat
     2041 gatgtccagg gtcagctcgt acgggccgct gtttttcgac cggatgacct tggccacgga
     2101 taatatgctg cgtttcatgg gacggctcct cgtctcaaat ggtgacgatc ttgaccggga
     2161 aaggggtccg gccggtcaac ggcatcaggt ggtagaccga aaattcgaac gtctctccca
     2221 ttctgacatc cgaaggcgaa aaaggaaagg ccaggttgcc cgccgtcgag atccggcccg
     2281 ggtagccgta atggaggaga gtcgagcggg tgatgctgca gagcgaatcg gccgcctccg
     2341 gcgtcgatcc gacggcctcg atgacgatcc caagctcgtg gctccggatc ttcgtctcgg
     2401 gctccaaggg gcccatgacc ccgtccttgc cgtagacgtg gaactggatg cggccgtcga
     2461 ttttctcagc cttgaggatg tcgcggactt ggccggtgac ggcttccagg atcgcgtcga
     2521 tctccttgat catgatggga tcccgcgtgc cggcgaccga gatcgtccgg aaaccggtcc
     2581 gccgaacgcc ctcgagcttg acgtaatatt cgggcgtcgg gacatggcgg cttccccgaa
     2641 cctcgacccg gccgccgggc agggcggtga acgtgcaggc ggtcagatcc agctcgccgc
     2701 cggggcctgg aaggtggtag ggatcggttt tttcgtagag ggtgtgggcg gccgtggatt
     2761 cgggagtgaa cgtccgggcc ggattgagcg tttccaggat gaaggaatcc ccgcggagaa
     2821 cgcccagggc gcaatccgcc cccgagcccg gggtggccgc gatggccgcg cactcgagga
     2881 tcttgcccat gtggagggcc aaaccctcat cgaatccccg catgaccgga agggcggcga
     2941 agaccgccgg atcatagcat cgtccggcca ggacgacctg gcatcccctc cggagggcct
     3001 cttggatcgg ttcgacgccc atctgggcga cgatgtggag ggagtcgtcc agcatttttt
     3061 ccgtcagggg cgggacgtag gccaggggga cgatttcccc cttgcgaagg tgtttccgga
     3121 tgcgctcctt cggaatgtcg gcgtagatga tgcccagggt gaaggtcaac ttttcctccc
     3181 gggcgatttc ccggatgatc gcctcgcacc aatcgacatg gggacgggcg ccgctgccgc
     3241 cggccgtgcc gatgaccacg gggatgcccc gccgcacgcc ttccctcagc ataaaacgaa
     3301 ggtcgcgctt gacgccggac cgattggtga aggccttccc ggctccgaga taatacgggc
     3361 cggggtcggt cgagccggcg tcggctccga tcaagtgggg tttgcgggcc aggccggccc
     3421 ggaacgattt ctccggaaag ccgtagccga gaatggcggt ggcggaaaga acacggaatt
     3481 ctttttcacg ggaagtcatg gttatttccc ctcagggcgg gcccgaagca ccgccagaat
     3541 gcgctccatc tcgttgcgga cgatcataaa gccctcgtcg aatcccatcc cgggcttggc
     3601 cagcagacgt tcggcccggg cggccacggc cgcgtggacg caggcgcggg ccgagacgtc
     3661 cgactcgttg caggtgccgc cctggtaggc ttccatgccg tgcctccggc agtaaaggac
     3721 gctctcgatg gtgttctgga tcccgcccag gtcgggcgtc ttgatttgga ccatgtcgca
     3781 gcaggcggcg tcggtgaaat cgcggacgtc ttccaaggtg ttgcaccact cgtcggccac
     3841 gatcttgacc gaggagccgc gccggcggag agtctcccgg accttcccga gaagctcaat
     3901 ctgccgggcc ttttcctcgg cgtcgaccgg cccttcgatg tacagcggga actcggccga
     3961 atcctcctcg agcgaggcga ggtaatcggc cactcgtccc gggtcgagcc cgaagatttg
     4021 tccgatcgtc ccgtagacgt cgatatggag ggtcggacgg taggagggtt cggtccggaa
     4081 cgcgcggatc cggtcggcca gccagcggat gtacgcccga agcagctccc cgttccgacc
     4141 gagcttttcg tcgacgttgt tgatcagggc gtggggcagg acgtccacgc ctttgaggat
     4201 catcttgtcg gcgttgacgt agcggtcgtc cccggtctgg ccgaagacgg ggacgcgctc
     4261 ggcgacgacc gggagattgt attcctcgca gatgatctcg gccatgagcc ggtgacggtc
     4321 gaacgcccgg gcttggagga gggcttggga cagtccgtag cggacggcgg tatggagccg
     4381 cacgccgtcg aattccaggt tttcgacctc cccggccatc gtccggaacg gtccgatctc
     4441 ccgcccttcg agcagcggcc ggatgcgcgt ctcgagcagg ggcaggtaat ccgcggccag
     4501 gaagagcgga tcgcgccctc cggctccgga atactgaacc gcggcgcaat cccctccggc
     4561 gacggctccg ttctccagga cgaggagaat ggacaagatt tctccggcca cccgtacgga
     4621 cttgaaatgg ggcgtggccg gctgcccggt gtaaaaaaat ccgtcgtggc cggccccggc
     4681 cttgatggcc cgctggtcgt cgaaatagaa ggcggacgac ccgggcacga acagcgtttt
     4741 gacaattttc atttcggcct cccgatcagt ttgctcttgc tgatggcata gatatcatcg
     4801 gtcaccatct ggaaggtgac cgcccggcct tcggccttgg ccctctcggc caggcgttcc
     4861 ttgtggaagg ccatgatctc gtcggacaag ggaagattgc ctttggcgaa gaggcggata
     4921 aacccctcgt tgtcgcgggc cggaagaacc cggccgcggt tgaagatgga cggggcgaac
     4981 ggcacgtcca gcaggccgcc ggcgaacgcc cggacgatcc cttggatcgg gtcgccgccc
     5041 gaggcgttct ccacggcgtt catcaagcaa gccacttcct cccggatcaa gttcttctcc
     5101 cggcggatga tgtcgttgtc gccgcagatt atttggtccg tcagcatgga taaaatctgg
     5161 cgggtggttt tgaggccggc ggcgttggct tccttggtcg gaatcccggc ggcctcatgg
     5221 ggcgacttga cgatgatttt ttccacgccg gccgcggccg cgacgaacga gccgaggctg
     5281 atgacggcga acgccttggc ctcgtcctcg ggaaatccgc ccatccactg atgaaaaacg
     5341 atcgtcaggt cgaaatcggt gaaaccggcc ttttcgaaat attcgccggc cagttcccgc
     5401 agcgaggaga gggcggccag gtcctggaga acgttgccgc cctggccgta gccgagagtg
     5461 atggatttgg cgccttgttc gagggccagg agaccctcca ggatggccac ggtgtgtccg
     5521 atgaacggcg gcaggagggt tccggtcagc ggcccgaacg gctcgcggtt gatcgagacg
     5581 ccgttttcct cgtaccagcc gaccagccgg tcgatatagc gccacgcccg gagcgagtcc
     5641 ccgagctcga ctttcttggc gtacgggatg ttgtaggaaa tgccgccgcc ctcgaagctg
     5701 gtgaatccgc cggccagcga gatctcggcc aggagccggg cgtcaggcgt gccatggcgg
     5761 acttggatcg gtttggtcac ggcttcggtc acccggcggc agccgcccag gccgtggttc
     5821 accgccggaa agccgttgag aagggaatgg ccggcggccg tcgatttctc gatcccgacg
     5881 gccgcttcct cgtaccggtt ctggcgggta taggcgtcga tcgtcgtcgg aagcaggtcg
     5941 gccgtgccct cggtctccaa gaagcgcagg agacggatgt gatcatcgat cagagccacg
     6001 cccgcccggg gctggatcaa ggtccggccc ttggccttgg ccccgctcat cgcccgggcg
     6061 aagttcttcg cctcgggaat gtcccgttgg tagcggagcc ctccctccaa gtcgacgtcc
     6121 ttgccggtcg gccaggttgc cagaacggat ttgcgctggg tcaagaaatc cttccaggcc
     6181 atgggttcgt atctcatgcg ttcatccttt taaaacacgg agtccgccga cggccgtccg
     6241 ggcggcggcg tcgggataat ccccggccag gttcgccagc agggggaaga gatacccctc
     6301 atcggcgtaa gccgcggggg aaaccggaag caggggttgg gaatcggcgt cggcttggcc
     6361 gggggcgcag gcctccagga gaatcgcggc gttttcggtc cgggagagat atcccccggt
     6421 cccgatcaag cgagtgatcc ggcggagatc cttgccccgc tgaacgtgaa agccgccctg
     6481 gggcgtgtaa acccggctga gcgttccggc gtgacggaga gcggccgccc ggacgcaggc
     6541 ggcggccatc atctcgtcat acgcccgctc ggcgccgcct tcgggccgcg actccggttg
     6601 agcggaaacg cggtggatgt acgcgtcgaa atcctcgatc gaacggccgg cggccgccag
     6661 gcgcgaggtc aagtacgccc gctcggattc ccacaaggcg gcggccgaga cgcggagtcc
     6721 gaggtcgccc tcgaccgtcc gcttgagaag gggttcgtgc aggccgcgaa gcaggaccga
     6781 atcccctccg gtgaaggcct ccgtgtggga gtagaagtcc gtggtcgccc cgcccatgtc
     6841 gacgagaccg aaatcggacc agcccggctg ggaggccccg atccggcgga cgagctcgaa
     6901 gacggccagc ggcgtcggcc agggatcgcg accggtctcg cggacgacgg tcgacagtcc
     6961 cttgccgtcg acgatccgct tgaggaagat cttccggatg acttcgcggg ccggctcgat
     7021 ctggatcgtc ccgacctcgg gcatgacgtt atcggtggcg gccgccgcct tgccgtcgag
     7081 caaggccagg acccggtcgc ggaccttggc gttgccggca tagaggatga acgcggcggt
     7141 cgaggaccgg cccaggagct ccgcgttgcg gaggacgacg gccgaattgc ctccgtcggt
     7201 gcccccggtg aagaggatga tgtcgggttg gagggcttcg aggccggcga tctcgtcggc
     7261 cgcgagctca taggaatacg tccggacgat cttgcctccg gccgaatacg cggccatccg
     7321 ggcgatcgaa agagtcaagt cggccgtcag cccgagagcc gcgatcttaa gaccgcctcg
     7381 ggcggaggag gacaagtaaa ccggaaattc ctccggccct cggccgccgg ggccgagttt
     7441 ctcccggacc cggccgaaac cgacggccag gttgtcggcc gtcgtcggcg tctcggcccg
     7501 ggagagaagc cggaaagccc ccgcctccag ggcgaacagg gcgcccttgg tgtaggtcga
     7561 cccgatgtcg atcgaggcga gggccgaggg cgtcatggac ggctcgcggc ggcgatatcg
     7621 gctttcagca tcttgacgac ctcggccagg tcgacctggg ggcggaagac gcggttgaag
     7681 cccatgtcca agaacttctt ctcgacctcc tcatagggcg ttttgccgac cacgaggttg
     7741 ccgccgacat agaggagaat gcggtccagg ccgcgctcga tgcatttggc ccgaaggcct
     7801 tcgcagtcga tttctccgtg accgtaaaga gaggagacga ggatggccgc ggcatccgtc
     7861 tcaacggccg cgtcgataaa ttcctcctgg ctgaccatca ctccgaggtt ggtcacatga
     7921 aaacccgcct cggagaaata ggcatccagg atcttgttgc cgaccgagtg acagtcggcc
     7981 ccgatgacgc cgagaacgat attttgcgga ttcatcagac gctccttttt tcctcgtcca
     8041 ggagcgttcg cagccgctct tcgacatgaa ggagcgagcc ccgctcgatt tggtaatcga
     8101 aggggatgat gatcccgccc cgctcggtga acgcctggag agcgggcaag tgctcccggt
     8161 ttttctgcag ggaatacccg gccggaacga tgaccgcgtc gagcgcggcg atgaactcgt
     8221 cgagacgctc gagctgggag atgaagaggg ttttgacgcc ggtggagggg atgtcgaacg
     8281 tccggagatg gtccaggatg atcgcctgaa actttctcga ttcgctgagg atgccgatcc
     8341 gctgagccgg cgtgagccgg gccaggctga tgatggacgt ccgggtcggg gcgacggcca
     8401 cctggacggc cttgctccgg atttcgggga agcggtcgat caattcctgg taatgggtgg
     8461 tggtcgtcaa aacgaggtcg aagatctcga gacgggcgcc cgccccggga tcctcgaaca
     8521 agtcgtccag gtcgatccga gccaggttga cgttggggat caggagaaac tggcgctcga
     8581 aaatcgtcag ggtttcggga ttgcaatcga tggcggccac cgagagattc cgcaggctgt
     8641 cctcccggcc cagcaggcgg aggtcaaaga acatccgaat gtcctgggcg gaaaacttaa
     8701 ggcgacccag ttgatccaat agctcgtcga tcagccggat cgcccggtct ttgcgatcct
     8761 cgaccggagc gaccgcttcc ccggcgacga aagatccgcg gccggaaaac gcttcgatga
     8821 gcccgcggcg gacgagctcc tcgtaggctc ttttaaccgt tccccgggcc gtcccgagtt
     8881 cctcggcgag ctcgcgctcg ggagggagct tgtcgccggg aagaagaacg cggtttttta
     8941 tctgctggtc gaaaaaggag atgatttgct gataagcggg cggagcggaa gagcttttgt
     9001 ccagcttcat cttttccatg atttcctcca aatggactaa tccaccttat aatggcctaa
     9061 tccttctata gaccattttt accggcatgt caagaattgc tggaaacacc ggtcgttccg
     9121 gagtataata ttttttatga agaaaggcgg atccgccgcg gggcgatacc gatccagccc
     9181 ttggcatacg cttcaattcg aatctccgac tcctcttcgg gcgagggaaa tcctggaagc
     9241 cgccgaatcc gccgtctcct cgggccgcct tccggcggcc gcctcggcca aggaatacct
     9301 gaacctgacg tcgagccgga cgtttcttca ggcgctcggc gaaccggacc tgttgaaccg
     9361 atgggcggag acggtcttcg ccttcatccg tctcaccggc tacaacctcc tcgacttgtt
     9421 cgaaagccgg atccgggaac agcctcacaa gatccttttc caagacatgt ccgcgtccac
     9481 gcccgtccaa tggacctacg aacaggtggg ccgccgggtc cgggagctcg ccggctcatt
     9541 ctacgccatg ggccaggagg acggagcgga gccccgggtg gcgatctacg cggaaaacaa
     9601 cgtcgaatgc gcctcggccg atttggcctg cctcctttac gacattctgg acacgccgat
     9661 caatccccat ttcaacatcg acaacttggt cgagatcttc gacgctctga acatcacgat
     9721 cgccctcacc gaccggccgg accgccggaa aatcctgggc gaagtccgcc gccggacggc
     9781 ccggccgttc cggatcttga ccgtggcccc ggccgggaag ccggaaaatg aaaaggacgg
     9841 cgtcctcatt gaatatgcca aacgccggcc ggttcgttcc ctcgaatcgc ttctcgaggc
     9901 ccgccgccgc cggccggtca acgaagtcgc cacggtcatg ttcacctcgg gctcgaccgg
     9961 gcggccgaaa ggcgtgtcct tttcttccta ccacctggtc tccaagcgct tcgcccgggc
    10021 ggcggccctg cccgacgtcg gcgacgacga ggtcttcctg agctttcttc ccctctttca
    10081 cacgttcggc cgttacctgg agcttctcgg catgatcttc tggaggggga catacgtctt
    10141 cgccgggaat ccctcggccg acacgctgtt caccctcttc cccaaggtca atccgacggg
    10201 attcatcagc gtccccgtcc gctgggccca gctctacgag aggtgcctgg aaaagctgga
    10261 cgagacctcc ggcgccgacc ggaagagcgt catccgctcg gttgtcggcc agcggctccg
    10321 ctggggcctg tccgccgccg gttacctgga tccgagagtc ttccggtttt tcgaacagga
    10381 gggcatcaac ctttcgagcg ggttcggcat gaccgagggc accggaggaa tcaccatgac
    10441 tcctcccggc cggtacgtcg acaacaccca cgggctgccg cttccgggcg tccgcctccg
    10501 cctcggccgg gacagcgagc tcgaggcctc cggccattac atcgcccctt acctggaagc
    10561 ggccgcgccg gaaagcctca tccccttccc ggaaaatccg gagaccgact actggcttca
    10621 gaccggcgac atcttccggc ggctgtccaa cggctattac caaatcatcg accggatcaa
    10681 ggacatttat aaaaacaacc ggggccagac gatcgctccc cgcaaggtcg aggataaatt
    10741 catcggcgtc cccggcatca agcggacgtt tctcgtcgga gacgggcggc ccaccaacgt
    10801 gctgttcatc gttcccgacc gcagcgacac cgtcctggcc tcggccttgg tccaggataa
    10861 cggccgggaa tattaccggc ggatcgtcac cgcggccaac gccgacctgg ctccttacga
    10921 acgggtcatc aatttcgccg tcctcgaccg cgatttcgac gccgcctggg gcgaggtgac
    10981 tcccaagggt tccttcaacc ggaagcggat cgaggagaat ttttccggcc ggatcgagga
    11041 gctctaccgc aaaccttatc ttgagctggc cggccccggc ggaaccgtcc gcatcccgct
    11101 gtggttcttc cgcgacttgg gcatcctgga ggacgaaatc acgcgcgacg cccgcggcct
    11161 ccgcgaccgg aaccggaagc tccgcctgcc gttgcggccg acggcccggc cgaaagtctg
    11221 gctggtgggc gatctggaat acgaaatcca ggggacggtc gtcgatttag gcttgttcgc
    11281 ccgccagccg attctttggg ccggcaatcc cgccttgatc cgcttttccc cctgcaagga
    11341 aggctgggac ctgccgctgg agccggcgac ggaccgcgtc ttccttcccg accggcgcgt
    11401 ccgtctcgcc ccgacggagg atatcgccca gccgtccaat atccggaaca ctcccctcct
    11461 ccgcctcaat gaaatcctcg tccggatcct attttccgga cggaagccgc tcgacggcct
    11521 tctcgacgag ctggaaaagc ttttccagga gccggatctc cgaaccacga acctcatccg
    11581 cagccgtctc cgggcccttg ccggccatcc ggccgaagac gtccgctgct gggcctaccg
    11641 cctccttctc ctcgacaatc caaacgtcga ttacagccgg cttctgccgg ccttcgtcca
    11701 gtcgggcaag ccgttcctca atgcccggag catcgaggga atcgtctcgg cccggccggg
    11761 caaaagccgc ctggattcac tccgccggcg gatgatcgcc taccgggaaa cgatgacctg
    11821 gccggccaat ccggtcaccc gactccaatt cgaccgcatc ctccggctgc tggcgaacct
    11881 gacccgggtt cacccggaat tcttcggctc catccgggcg gagctggcgg cctgggctct
    11941 tcatacggcc gatccagggc tggccgcctc ggccgaaaag ctcctggcca agctcacaca
    12001 cgggatcgaa aaagacggca agaaagccgc cttcggcgtc ttgagaaaag aagaccggct
    12061 ggttttcgag gaagggctcg gtcccgccga gatccgccgg ttgaaaacgc ttctcctcgg
    12121 gacgacgttc ctccacgagt ccatccggtt cgccttcggc ccggatgatt tcaacccgag
    12181 ggacatcccg gccgccggca tttggatttc ccgggcccat tccgtctcct ccttccccga
    12241 ttaccggatc agcctgaata cgacctccag ccgccattat gacctgaagc ttttcctcaa
    12301 agggcggccg tggtcggacc gcgaatggcg gacgattctc tggtcggcct tcctggccga
    12361 ccacccggag gagacggcgg ccatggctcc ccttggggca atccggcttg gggaaggcgc
    12421 cgtctcccgg agatacatcg gcgacctgac cctctgggaa aagatccgtg aatacgccgg
    12481 ccgccgggcg gcgggcgcgc ctccgcccga actggcggtt tggcggaagc tctacattga
    12541 agcgatgtcc gtcttcttcc gggcctggaa aatgaccggc cgccggatcg tgccgggccc
    12601 cgtttccccc ctcaacgcca tcgtcccgga aatcgagtac cgggacgcga cgatcatcgc
    12661 ttctctcgac tcccggacgc cgtaccggaa cactctgtcg attgtccgtc cgctcgtcga
    12721 ccgcttttac cgtcggacgg ccgccgatta tccttggatc cgggagcaaa cggacttgga
    12781 atggattttc gacgcctgct acaacgccct gggccgtgat gaagcggcgg cctttttcgt
    12841 cgaacttcaa aacgacctcg gcggggggaa gcccgcctca ccggagctgg ccggcctgcg
    12901 ccggctgctg gccgagtacc gcgcccggtt cgaaaaaagc taccgaatcc cgctggccgg
    12961 gctcaacgcc gtccgggcct ttcaggaatg ggaggcggcc aatccgaagg ccgactcccg
    13021 ggaccgggaa cgaaaggtcc tcgatcttca ccggaaatac gacctgggcc gcttcccgga
    13081 gatcgtccgc taccatctct accgccacac ctatttcgcc gggcggagct tgaaggagga
    13141 ggagctgttc gaccggctcc tggccttgat gtcggagaat cccaaggccc cggccgtccg
    13201 gcacgtcgag ctgtccgatc tccaagccgc cttgaacgac gagagggacc gggccgtctt
    13261 cagccggatg atttttcccc atctcgagcc ggaccgccgt ctcgacgtcg tcgtcgaggg
    13321 cgaagagggg gcggagcgga tcgtcgtccg gagctttctt gtcgaccgcc ggggcgaaac
    13381 gtacgccttc agcgaaacaa ccgacccggc cgacatcggc cgcctctatc ggctcttcta
    13441 caaggagaat ttccccaagg tcatttccca acaagaccgg catttcatcc tccaagaccg
    13501 ccagaaccgc atcgtcggag gattgtgcta ccgggtcatg tcccggtccg tcgttttcat
    13561 cgatgcggtc gtcatcacgt cccggctgaa gtcaagcggc ttgggcggag ccgtggtgga
    13621 cgatttttgc gggcggatgg ccggccaggg atacacgacg gtcctgaccc atttctacct
    13681 tcccggcttt ttcctccgcc ggggattccg cctggacaag aaatgggggg ccctggccaa
    13741 gtcgatcggc taggattcga aattcccggc ggcttccggc tggtagagga ctttcttgat
    13801 cttgaattta cggagtccgc ccggcacctt ccactcgacg acgtcgccct tgcgaaatcc
    13861 gagcagagcg gttcccaggg ggaccaggac cgagattttt cccacgctga aatcagccgc
    13921 cagcggaaaa acgagcgtgt attccttttc ctcgcccgtc gcgacgtcct ggataaggac
    13981 ccgcgagttc acggtgacga cgttcgggtc gatttctttc ggatcgacga cgacagcccg
    14041 atcaagctcg gcccggagct cctggaaagc gtttttatcg agatccgagg gatccggatc
    14101 gacggaagtc aacgccttca accggtcata gtcggtttgg gtcagggtga tggtcttggc
    14161 catgggagac tccttcgcct ttttttcgtg attggatgac aggtggaccc gctgtcaatg
    14221 gcaatataag tcaagacagg gaattatgca agccgaacgg ctcgtggtag aatcgggaac
    14281 aatgaagagc aagagagcga aatcaagacc gcccgccctc cagggccagg ttcaaatcta
    14341 caccggtgac ggaaaaggaa aaaccacggc ggccctgggc ctggcccttc gagccgccgg
    14401 ccgcggctac cgcacgtatt tcggccagtt catgaaagcc cggtcctcgg gagagcgggc
    14461 cgccgcccgg cggctcggcg gcttgatcac gttcgcgacc ttcggccgtc ccggcttgat
    14521 ccggaccgcc ggccgtccgg atccggaaga cgtccggcgg gcgcgccgcg gactggacct
    14581 ctgccggaag gcgatgcttt ccggccggta tgacatcgtc gtcctggatg aaatcaacgt
    14641 cgcccttcac ttcggcctcc tcggcctgcc ggacgtcctg gcccttctcg acgagaaacc
    14701 tccgtccgtc gagctcgttt tgaccgggcg ccgggctccg gaagcgctca tccggcgggc
    14761 cggcctcgtc accgagatga aggaaatcaa gcattatttc caggccggcg tccccgcccg
    14821 ccacggcatc gaggattgat tccccggccg cgaaacaaag aaaattccat ggattccctc
    14881 tataaaagag gcggattcgc ggccgaaatc atggaccagt ccaaaacatt gacttttcag
    14941 gcgcggaaag gcaaaatacg ccttccaacc ccttgtttta taaacattta ataatcgaca
    15001 agggaattgc cgtatggagg attcatccat gaccctgctc gagagtctca aggaaaaagc
    15061 gaaggcgacg ccccgccgca tcgtccttcc cgaaggcgag gagccccgga cgattaaggc
    15121 ggccgcccag atcgccaaag aacagctcgc ccaagtcact ctggttggca accgggcgaa
    15181 gatcgaggcc gtggccaaag aacaaggcgt cgacgtctcc gggatcccct cggtcgatcc
    15241 ggcggcgtcc ccgaagttcg aggaatacgt ccaggcttat tatgagatcc ggaaagccaa
    15301 gggaatgact cccgaggaag cccgcaagct catgtccgac ccggtctatt tcgggacgat
    15361 gatgctccac aagggcgaag cggacggcct cgtctcgggc gtggtccact cgaccggcga
    15421 aaccatccgg ccggccctcc aaatcatccg gaccaagccg gggatcaaca tcgtctccag
    15481 ttcgttcgtc atgatcgtcc ctgactgctc ctacggcgac aacggcatgt tcgtcttcgc
    15541 cgactgcgcc gtcatgcccg ccccatccga cgtcgagctg gcccaaatcg ccgtcgcctc
    15601 ggccgagacc gcccgggttc tctgcggaat ggatccgaag gtggccatgc tatccttctc
    15661 gacctacggc agcgccaagc acgagctcgt ggacaaagtc aagaaggccg tggccgtcgc
    15721 caaggagctc gcccccggcc tgaagatcga cggagaaatg caggccgacg cggccattat
    15781 cccgaaaatc ggaaaggcca aggcgccgaa ctcggcgatc gccggccagg ccaacgttct
    15841 cgttttcccc gacctccagt cgggcaatat cgcctacaag ctcgtcgagc ggctggccaa
    15901 ggcccaggcc gtcggaccga ttcttcaggg catcgccaag ccggtcaacg acctgtcccg
    15961 cggctgctcc gtcgacgaca tcgtccatct cacggccatc accgccgtcc aggccgccgg
    16021 cctgtaagcg gtcaagaata gatcgaacgc gtcacatagg agaattccat gatcgttctc
    16081 tccctcaact gcggcagctc ctcggccaag taccgtctgt acgattacga caagaaggtc
    16141 accctggcca ccggcatcgt cgagcgcgtc ggcatcggca aaagcttcat tcagcaggcc
    16201 ggtctcggcc gcgcgccgat ccagatcgac cacgactgcg ccaaccacaa ggacgccctc
    16261 aagctcatca tcgacacgct gctcgacccg aaggtcggcg tgctcgatga cctgaagaag
    16321 atctcggccg tcggccaccg ggtcgtccac ggaggcgaaa agttcaaaaa atccgtcatc
    16381 atcgacgccg cctccctcga cacattcaag gctctctaca gcttggcccc tcttcacaat
    16441 ccgcccaacg ttctgggcat cgaggcagcc atggacgtcc ttccgtccat tccccacatg
    16501 gccatcatgg acacggcctg gcaccaaacc atgccggccc acgcctacat gtacgccctg
    16561 ccctacgagt ggtacgagaa gtacggcgtc cgccgctacg gcttccacgg cacatcgttt
    16621 ctctacgtag ccaagcgggc cgccctgctg ctgggcaagg atcccttcaa gaccaatctc
    16681 atctgcctcc atatcggcaa cggcgtctcg gccaacgcca tcaagaacgg cgtgtcgtat
    16741 gacacctcga tgggcctgac gcctctggaa gggctggtca tgggcacccg cgccggcgac
    16801 cacgacgcgg ccatcgacct catgatgatg gagaaggaga acatcgcacc ggccaagatg
    16861 aacgacctgt tgaacaagaa gtcgggtctt ctgggcatca cgggcaaata caccgaccgc
    16921 cgcgatgtcc agaaagcggc cgaggccggc gaggctcggg ccgccctggc catcgagatg
    16981 gagtcctacc ggatcaagaa atacatcggg gcctacctgg ccgccatcgg cggggccgac
    17041 gccattgtgt tcacggccgg cgtcggcgaa atgtccgacc tcatccggga aaaatctctc
    17101 gacggcctcg aattcctcgg catcaagctg gacaaggcga aaaacaagct ggccaaaacg
    17161 agaaacgccg aaaccgagat ttcggccgaa gactccaagg tcaagatttt cgtcatcccc
    17221 accgacgagg aacgggtctt catcgaggac gtcatcgccc tgctccaggg gacgtacgac
    17281 gaacacaccc ggttcactta ccggttccag catcccgact acgtcaattc ccagcgggag
    17341 caggatttcg cggccgattg cgcgaaaaaa cccggcttgg ataaaatcca ggtcaagccg
    17401 aaaaaataaa aatccggttt ccccgcgctc gtcccaagcc ccgtccctcc gcggacgggg
    17461 ctttttttag gaaggaccgg ggcattctct ttttccaaag aagcggaaaa gggtgatttt
    17521 acggcgggac ttttgatatg atgaagggca acggcctggt ctcggccggg gagaaggatc
    17581 acatgaaagc cgcctttttg accgccccgc ggaaagtcga aatccgcgac gtcccgaaac
    17641 ccctcctcaa gaacgacgac gatgtgcttc ttcgaacggc gctcgccggc ctgtgcggca
    17701 gcgatcttca ctatttcacg gcggaccgca tcgggggcga agcggtcccc tatccggcgg
    17761 tcgtcggcca cgagtgctca gccttcgtcg aggccgtcgg gccgtcggtt aaacagttca
    17821 aagccggcga ccgggtggcc gtggagccgt cgatctcctg cggaacgtgc gaccaatgcc
    17881 gggccgggcg gccccacacg tgccggcatg tctcttttct cggccacccc ggcgagcgga
    17941 gcggctgcct ggcggaatat ttcgtcatgc ccgaacgcaa tcttttgtcc gtgcccgaga
    18001 cgctgacgat gacggaagcg atgctggtcg agccgttgtc gatcgccctc cacgcccggg
    18061 ccctggccgt cggaacgctc gggccgtcga tcggagtgct gggaacggga ccgatcggcc
    18121 ttttactcgt ccaggttctc aaaaccgagg gccacgggga catctttgcc accgaccgct
    18181 ccgacgcccg gattgcggcc gcgctcaaag ccggcgccgc ctggagcggc aatcccgacc
    18241 gcctcgacgt cgtctcggag attttcaagc ggaaaccgct cgggctggac gccgtcttcg
    18301 aagcgtccgg cgacccggcg gcgatcgaac aggccgtcga gctggtcaag cccggcggct
    18361 ggatctacca gattggaatc cccccggacg aacggatttc ctacctttcg gcccggctgc
    18421 ggcgcaagga gatcggcctc cggttcctcc ggcggcagaa ccggtgcgtg aatcagggac
    18481 tgatgctcgt caaggccaag cacctggaca tcgactggct ggccacccac accttcaaga
    18541 tcgaggaggc ggaaaaagcc ttcgccacag ccgccgaccg gaccgacggc gtcctcaaat
    18601 cctcgatcgt tttttagtcc gcctcattcc cgaactggga cgaccgcggg cccgccccgc
    18661 ggaacgcgaa ggggccgggc gggcggaatc cgctggggcg ttttttcccg aaaccgcctt
    18721 agaagcccag atgctcggtc cggcggatga tctcgttttg caggtcgacc gagcaatcca
    18781 ggaagtaatc gc
//
LOCUS       DAOOXH010000046        18579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_2874_length_18579_cov_7.018355, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000046 DAOOXH010000000
VERSION     DAOOXH010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 18579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18579
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2874_length_18579_cov_7.018355"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="PKV64_06770"
     CDS             <1..1049
                     /locus_tag="PKV64_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPN16819.1"
                     /translation="SDDAVRDYVRFIRKISDEGGLLPFDRTGMAAVIEHGIRLSGRRE
                     KLSTLFHRLADVIREADYWARKDGQETVQGVHVEQAVRERFERINLVERKIQEMIAEG
                     TILIETSGGVVGQVNGLAVYSMGQAAFGKPSRITARTSVGRAGVINIEREADLSGQTH
                     NKGVLILGGYLRGKYAVDRPFALSASIAFEQSYGGIDGDSASSTEVYAILSALSGLPL
                     RQDIAVTGSLNQKGEIQPIGGVNEKIEGFFDVCRAQGLTGTQGVMIPVQNVRNLMLRR
                     DVVAAAAEGRFHIYPVRTIDEGIEILTGVPAGRKNETGTWEERTVNFLVDRELERLAR
                     ASKGFFEERNKEKP"
     gene            complement(1224..1970)
                     /locus_tag="PKV64_06775"
     CDS             complement(1224..1970)
                     /locus_tag="PKV64_06775"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPN16820.1"
                     /translation="MIRLNRFLAQAGVASRRKADGLIADGRVAVNGDVVEDLGTKIDE
                     SSDRVTVDGRPVRVRGRAVYWMLHKPAGYLVTLDDPFERRTIRDLIPDLPGGVFPVGR
                     LDRDSEGLLILTSDGELAYRLTHPRYEVAKRYVVHVRGEMDETAAGRLRAGVFVEGRK
                     TAPAKVEVFERSARGSVLQIEIHEGRKHEVRNMCEAVGHPVVSLKRVMFAGLSLSHLP
                     AGDHRSLTGAEILRLKKAAKIGKIGRHNTA"
     gene            complement(1989..2549)
                     /locus_tag="PKV64_06780"
     CDS             complement(1989..2549)
                     /locus_tag="PKV64_06780"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPN16821.1"
                     /translation="MKRRLIIPVVASVLVFALPARALVGVYAGGFGGVSSQKLSFDDV
                     NFDADTTFLYGVRAGLQAAVFALEFNYYRAGHNVAMQDFFLFNWDGLENDTSFIGGNL
                     RIMFPVAVFRPFLALGYGYYTADVRNIDKDTEAGFNFGAGLEIKLGKLGLIGEGKYNL
                     VNVNLDEMDFGLRHFTFTVGLHFYFF"
     gene            2893..4128
                     /locus_tag="PKV64_06785"
     CDS             2893..4128
                     /locus_tag="PKV64_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN16822.1"
                     /translation="MAKINFGESLSLAFSSLWESKLRTFLTLLGIIIGVLTIITVVSV
                     IQGLNNYVYTKMAFFGANDFSVSKFSSFASTVKEYREQMKRRDLTLEDVRLLREKASA
                     CELIGASDQVRRTVKFGSAEIQNAGIIGVTAVDHLIGSVIELESGRHLLQEEEDRSRF
                     SAVIGSDVAEKLFPRLDPVGRTIKVGADRFRIVGVGKKLGKILGMSRDNYVRVPITTF
                     LKTFGSRRSLSINIHTSSPEAMARAQDEVRTILRSKRKLSYDKPDDFSFQTSETFIQF
                     YKTATNGIYFAMIGIASIALLVGGIVVMNIMLVAVTERTKEIGIRMAIGARRKDILVQ
                     FLIESSAIAGVGGLIGIVLGVAAAKIISAATSLPSAVDPGSVIAGIVMSATLGLFFGI
                     YPANKAAKLDPVVALRSET"
     gene            4125..5369
                     /locus_tag="PKV64_06790"
     CDS             4125..5369
                     /locus_tag="PKV64_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN16823.1"
                     /translation="MRLGIFREIVAMAFDSIRTHKLRSFLTLLGIMIGVMTVIGMVSI
                     IQGLNASFLQELQSAGSDLIIVHKYDPVAMRERTAEERARPDLTYDDARAIEEECDLV
                     KAVSVALAPSFLDPIEIKYQNQKSEGSVVLGINDRFPAVYNIYAPKEGRFLTASEIAR
                     SARTCVLGSELADILFPHQSPLGKTIRIGPGNFTVAGVLAKRGQMFGQSRDNIVAIPI
                     TTLMKHFPYDPESIEIGIVPRKPGTIQETIEQIRATLRLRRKVPFGRPDNFSLFTQDT
                     MLDLYNQITGAAYLVMIIISSIGLLVGGIGVMNIMLVAAKERTREIGIRKALGAQSSD
                     ILRQFLVESMFLTGTGGILGMLVGFAVALIVRAATPLPAAVTGWSIFLGLFVSISVGL
                     FFGIFPAQKAARVDPIVSLRYE"
     gene            complement(5583..6809)
                     /locus_tag="PKV64_06795"
     CDS             complement(5583..6809)
                     /locus_tag="PKV64_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HPN16824.1"
                     /translation="MSETKRLYFEDAGRLEFEARITGRRTVDGRPAVILDETAFYPES
                     GGQPWDLGTIDGIPVVKVLDEEGTIVHVLEREPGQEAGEGAEKVKGLVDGRRRFDHMR
                     QHSGQHVLSQAFIEILNGETRSFHLGADASTLEIGIENITDETLDRVERRANAVVFED
                     RAVKTYFVPAGRIAEVPLRRPPKVSGLVRVVEIEGFDYSACGGTHVRRTGEIGLIKII
                     AAERIRGNLRFTFVCGGRALADYQVKTRVCRGLAGKFNVPDREIPAAVEKQGAELKDV
                     KKRLKTVEQAAAGYEARELAEGASGRIVARVLAEKSPEGARALALSLVKLGPAAAVFA
                     TRSGGKARLVLARADGVAADLRPLAGVLAPLFNGKGGGGPSLIEIAGDQEADLDALVA
                     RASSAVAEEIGRHNPN"
     gene            complement(6855..8072)
                     /locus_tag="PKV64_06800"
     CDS             complement(6855..8072)
                     /locus_tag="PKV64_06800"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HPN16825.1"
                     /translation="MIVLLKILAVIFALLFLVKLKVDLGIVLVLDAILTGVLFAMPAA
                     AFVKQAGAALIDPETLNLIGILILVLYLGRFLQAGGHFKEMVAALKNYVRDARLILAI
                     PSAFIGLLPMTAGAMMGAPIVEEAARRWTLSPAWKTFLNYWFRHIWEYSWPMYPNLIL
                     AATIAGIPIVKVCLNQFPFTVIAVAAGLAILFRHVRYERNEQASRPRVKDIVRTFVSI
                     WPIFLTILLIFIFRIPMLWALASACVLSAFFVKLPGRERWAVVKESLSLRMILLTAAV
                     MVFKRILETSGALDAVMTIVSPTGPSGIFLLFAAPFTIGLLTGVNQAFAGIAFPLLVP
                     VIGRESPDMVLFLFAYVSGFAGVLLSPAHLCLALTADYFKADLKDVYKILIPPVAVVF
                     GAALLALVVFRLL"
     gene            complement(8069..8728)
                     /gene="rnhC"
                     /locus_tag="PKV64_06805"
     CDS             complement(8069..8728)
                     /gene="rnhC"
                     /locus_tag="PKV64_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HPN16826.1"
                     /translation="MKQQDLIPAEPGRIGTDESGKGDYFGPLVVAGFYLPPDQEGVLR
                     ELGVRDSKAISDNRCREIAETLQRGYPHAVVAIGPERYNELYEKLRNLNRLLAWGHAR
                     AIENIMEKMDCRRAVTDKFGDERYVRNALLARGKTVTLTQMVRGESDPAVAAASILAR
                     AEFLKKLYFLGRDWGMEFPKGASSAVEAAAAEFVRRHGRESLAKVAKVHFRTTGKALG
                     S"
     gene            complement(8778..10163)
                     /gene="gltA"
                     /locus_tag="PKV64_06810"
     CDS             complement(8778..10163)
                     /gene="gltA"
                     /locus_tag="PKV64_06810"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPN16827.1"
                     /translation="MKLPRQPMPQRGAGERGRDFLEVNRGLSPEAAAAEARRCLDCAK
                     PGCVSGCPVSIDIPSFIKLVEKGEFIAAAFKIKETNALPAICGRVCPQESQCEAVCNL
                     KRAAGVPVAIGNLERFVADYERAAGEVALPELPPPTGRKVAVIGAGPAGLTAAGELAK
                     KGHAVTIFEALHEPGGVLIYGIPEFRLPKAILKAEVEYLRRLGVEIRYNFVAGKTAAI
                     DDLKARGYEAFFIGTGAGLPNFMKIPGENLVGVYSANEYLTRVNLMRAYDFPAYDTPV
                     FRAKNAAVIGGGNVAMDSVRTAKRLGAERAMIIYRRSRREMPAREEEVAHAEEEGIEF
                     HFLTVPIRYEGDAYGRLASMTCLRMELGEPDASGRRRPVPVPGSEFTAPVDLVVVAIG
                     QSPNPLLLETMPGLKVGKWGTIEVDPATMASSLPGVYAGGDIIRGGATVILAMGDART
                     AAASIDAYLKS"
     gene            complement(10289..11119)
                     /locus_tag="PKV64_06815"
     CDS             complement(10289..11119)
                     /locus_tag="PKV64_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPN16828.1"
                     /translation="MPRIIAQRRLVPNLHLIEVEAPDTARAAKPGQFVILMPDARGER
                     IPLSIADWNAARGTVTSVFLVLGTATRKLALLKSNDEIPVFVGPLGRPTEIANFGTVV
                     LAAGCYGIGALWPTARALRRAGNRVITVLDAKASYLVYWDDRYRRVSERLIVSSRDPG
                     IGGGEYVAEKIKALAAAEKIDRVMAVGCTAFMRSVSEATRPAGIKTIVSLNPIMVDGT
                     GMCGACRCTAAGKTKFACVDGPDFDGHEVDWDELFLRRKSYFDEEIQALCRWEKENLP
                     "
     gene            complement(11120..13345)
                     /locus_tag="PKV64_06820"
     CDS             complement(11120..13345)
                     /locus_tag="PKV64_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPN16829.1"
                     /translation="MKITIDGRTADVAAGATVLDAARAMGISIPTLCDHRRLVPYAAC
                     RICLVEVAGRRLPAPACATMVEEGMTVVTNSPRILDLRRDVLDLLLSEHPSACLVCLE
                     KNDCEESKATSRKTDEPTGCILCPADERCELQKVVAEVGLKRLSHPMAYRGTGVLRDD
                     PFIDRDDGLCILCGRCVRVCREIRGAEAIAFVGRGAPVSVGTSMGKRLLDVGCRFCGA
                     CVDVCPTGALAERAVRYGAKFNETRTFVCGLCGQGCRLGLRLRDGRPVDVRPQDGPPS
                     DGQACVKGRFIIGDLLGHPKRLLRPMVRRGGVLVETSWEDALASAAAGLGGLKGDIAA
                     VLSAQDSCEDLWSLGRLSLDVLGARSLHLAEDISVYRSGFPAGRPGGDILAPSFSFDD
                     LAKARLIVIFEEALPLTAPILGTAVNRAVLGGAKLVLVGGDGDSCLDRCASLKIKLGP
                     ADAGRFLLEVLRSVGENRSGRVPAGAPGLEELRRSLRELPVWSRAETVSAKTEKLIAL
                     LERRHPAAFVFGPAFVAGPWGPRNAAAVWNLAGMTGGLYVPAGWEANARGAVEIMADF
                     GASAGSASPRPRGLLSAGPARAFVRDGEEFVVVLGAFQGKAAAQADVVLPRTLFPEQA
                     GTYVNDEGRIQFSEAVLAPAGEARPARGIAGELARRLGAEIAAPAATAFFAKSRRSGG
                     EARPLFIGGASFAEMGFRAIPDRLTEFDPLAAGERGDPDDCLGFPLALEIKSLRTARR
                     R"
     gene            complement(13342..15066)
                     /gene="nuoF"
                     /locus_tag="PKV64_06825"
     CDS             complement(13342..15066)
                     /gene="nuoF"
                     /locus_tag="PKV64_06825"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPN16830.1"
                     /translation="MTRLQHPGHLEDWRKRLLEETTSLRRTIVVTAGTCGRAGGALEI
                     RDALQAEIDRLGAGDKVGLLVTGCHGFCEMEPSVVVQPEGIFYGRLKPADAAAIVKQT
                     GLAGKVVERLTFEIPSTGRRVARMDQIPFYKKQLRLVSEANLKVDPERIEDYIRIGGY
                     RGLAKGLFDMTSEGVIEEVKAAGLRGRGGAGFPTAVKWELCRRASGHPKYVVCNADEG
                     DPGAYMDRSLLEGNPHAVIEGMILGAYAMGAAEGFIYVRGEYPLAVKNVERAISQARV
                     CGLLGQNILGSEFHFDLQVVQGAGAFVSGEETAMMASIEGRKAFPRQRPPFPSESGLW
                     GKPTNINNVETWANVPIVLDRGSARFASVGTEKSKGTKIFSLVGQIRNTGLVEVPMGI
                     SLREIIEDIGGGVPEGRTLKAVQTGGPSGGCIPASALDLRIDFESLAKAGSIMGSGGM
                     IVLDDRTCIVDLARYYLHFLEEESCGKCVPCRAGTRRMSELLKRMTSGLAEEGDLARL
                     ESLALTVKTASLCGLGQTAANPVLSTVAHFREEYEIHIREKRCPAGVCRDLVSPPSAR
                     PAGKKERP"
     gene            complement(15063..15542)
                     /locus_tag="PKV64_06830"
     CDS             complement(15063..15542)
                     /locus_tag="PKV64_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPN16831.1"
                     /translation="MDRDWKSELDELLKDFGTRQRDTIPVLHEVQRHFDHIPPAALPV
                     IARRLRVSESELYGVLSFYKAFSLRPKDAHTISVCMGTACHVRGGDRIVEELEKVLGV
                     SAGKSTPDGMFTLETVNCLGCCAIGPVLVLDGVTHARITPAAARDIVGRIRTGEKPA"
     gene            complement(15615..17294)
                     /locus_tag="PKV64_06835"
     CDS             complement(15615..17294)
                     /locus_tag="PKV64_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPN16832.1"
                     /translation="MNGRRLLFGLLVASIAAFGANCRSGGAMADSIIINANVRTGDPA
                     KPAAEAVALRGDRILLVGSNAEVKRAASRGAEIIDAEGALVLPGFIDSHVHLAAGGFG
                     LLGPDLREAASKEEFVARVAARVRDVPPGEWISAGTWDNQKFQPVEMPRREWIDAVTP
                     DHPVCLDRMDLHTVLVNTAALRRAGIDRTTTDPPGGEIARDPATGEPTGILKDAACDL
                     IREVIPEPSSAQKKRAVEAALRAAAARGLTSVHDVAGEESLGACRDLLREGRLTVRVR
                     FYLPIAMIDRLADVDVRAEGGSGFLRFGGLKGFVDGSLGSQTAWFEAPYADDPSSSGF
                     PAAGMFPEGLMERRIRAADAAGLQTAVHAIGDRAVAALLDLYERTAADGRPRDRRFRM
                     EHAQHVRPADFPRFARLGVIASVQPYHLVDDGRWAEAKIGPERARTSFAFRSFLEAGA
                     VLAFGSDWPVAPLDPILGVWAAATRATIDGRRPEGWIPEQKISVEDAVRAFTAGGAYA
                     EFAERDKGRLVPGAWADLTVLDMDLFRVPPERVREARVLRTILGGRTVFEA"
     gene            17422..17988
                     /locus_tag="PKV64_06840"
     CDS             17422..17988
                     /locus_tag="PKV64_06840"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HPN16833.1"
                     /translation="MEKGQAMKSVFKSDPDKKRIAALFDGVFAVAMTILVLELKLPRL
                     QDGDSIGELWAALRNHGSTFFSYLLSFFILGSLWIVHSRIFGKLLRISGAALAVHMAL
                     LITAAFFPFSVHLYGSYTGNTLGGLIYFGTIILLYAEMLALVIVADRGRLFEPGVPRN
                     DVKKLRRGFLWTILLLAAAYILIYLFPF"
     gene            18042..>18579
                     /locus_tag="PKV64_06845"
     CDS             18042..>18579
                     /locus_tag="PKV64_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HPN16834.1"
                     /translation="MVNPTLRVSSPGRICLLGEHQDYFGLAIIAAAINLRISFSGRPR
                     SDRRIVLDLPDMGEREEFVLDGGQIPYVKKRDYLKSAVNIHLRAGLPMSGWDVEVRGD
                     IPINAGTSSSSAMVVAWNKFLLEAAGDPRASDPRELAELGYETEVAEFKEPGGRMDHY
                     ASAHGGVVWIRFEEQLVPG"
BASE COUNT         3243 a         6243 c         6007 g         3086 t
ORIGIN      
        1 gctcggacga cgccgtccgg gactacgtcc ggttcatccg gaaaatctcc gacgagggcg
       61 ggcttctccc cttcgaccgg accggcatgg ccgcggtcat cgagcatggg atccggttgt
      121 ccggccgccg ggagaagctg tccacgctgt tccatcgcct ggccgacgtg atccgggagg
      181 ccgattactg ggcccggaag gacgggcagg agacggtcca gggcgtccac gtcgaacagg
      241 ccgtccgcga gcgcttcgag cggatcaacc tcgtcgaaag gaagatccag gaaatgatcg
      301 ccgaaggcac gatcctgatc gagacctccg gcggcgtcgt cggccaagtc aacgggctgg
      361 ccgtctacag catgggccag gccgccttcg gcaagccgag ccggatcacg gcccggacgt
      421 cggtggggcg ggccggggtg atcaacatcg agcgcgaggc cgacctcagc ggccagaccc
      481 acaacaaggg cgttttgatc ttgggcggct acctgcgcgg caagtacgcc gtcgaccggc
      541 cgttcgcctt gtcggcctcg atcgccttcg agcaaagcta cggggggatc gacggcgaca
      601 gcgcctcgtc gaccgaggtc tacgcgattc tctcggccct gtccgggctc cccctccgcc
      661 aggatatcgc ggtcaccggg tcgctcaacc aaaaaggcga gatccagccg atcggcggag
      721 tcaacgagaa aatcgagggc ttcttcgacg tctgccgggc ccagggcctg accggaacgc
      781 agggcgtcat gatccccgtc cagaacgtcc ggaacttgat gctccgccgg gacgtggtcg
      841 cggccgcggc cgaggggcgt ttccacatct acccggtccg gacgatcgac gagggaatcg
      901 agattctgac cggcgtgccg gccggccgga agaacgagac cggaacctgg gaggagcgaa
      961 cggtcaactt cctcgtcgac cgcgagctcg agcgcctggc ccgcgcctcc aagggctttt
     1021 tcgaggaaag aaataaagaa aagccctaat catcgggaga cacaatacta attcacctca
     1081 tgtctccgtg ttttccggtc gacggcgacg caaaaaatcg tgtctcgtgt gtttgccccg
     1141 gggcgtgatt cgaggaatct ccgcgttttc tcaggcatac gccctaagaa cggagaacga
     1201 agacagccgt cggggaaata ggatcaagcg gtattgtgtc ttccgatttt cccgattttc
     1261 gcggcttttt tcagccggag gatttccgcc ccggtcaagg accggtgatc gcccgccgga
     1321 agatgcgaca gggacaggcc ggcgaacatc acccgcttca aggaaacgac gggatggccg
     1381 accgcctcgc acatgttccg gacctcgtgc ttgcggccct cgtggatctc gatctggagg
     1441 acggatcccc gggcggaccg ttcgaacacc tcgaccttgg ccggagccgt tttccggccc
     1501 tcgacgaaaa cccccgcccg caggcgcccg gccgcggttt cgtccatctc cccccggaca
     1561 tggacgacat accgcttggc gacttcgtac cggggatggg tcagccggta ggccagttcc
     1621 ccgtcgctcg tcagtatcaa caatccctcg ctgtcccggt ccagccggcc gacgggaaag
     1681 acgccgcccg gcagatcggg gatcaggtcg cggatggtcc gccgctcgaa gggatcgtcc
     1741 agggtgacga gatagccggc cggcttgtgg agcatccagt agacggcccg tccgcggacc
     1801 cggaccgggc ggccgtcgac ggtcacccga tcggagctct cgtcgatttt cgttcccagg
     1861 tcctcgacga cgtccccgtt gacggcgacc cggccgtcgg cgatcagccc gtcggccttc
     1921 cgccgcgagg cgacgccggc ttgggccagg aaacggttga gacggatcac ggccgggacg
     1981 aagggggatt aaaagaaata aaaatggagc ccgacggtga aggtgaagtg gcggagcccg
     2041 aaatccatct cgtcgagatt cacgttgacg aggttgtact tgccttctcc gatgaggccc
     2101 agcttgccga gcttgatttc gagccccgct ccgaaattga agccggcctc cgtgtccttg
     2161 tcgatgttcc tcacgtccgc cgtgtaatac ccgtagccaa gggcgagaaa cggacggaaa
     2221 acggccaccg gaaacatgat ccggaggttc cccccgatga agctcgtgtc gttctccagg
     2281 ccgtcccaat tgaagagaaa gaaatcctgc atggccacgt tgtggccggc ccggtagtag
     2341 ttgaactcca gggcgaagac cgccgcttgg aggccggccc gaacgccgta gagaaacgtc
     2401 gtgtcggcgt cgaagttcac gtcgtcgaag ctgagcttct ggctggaaac gcccccgaat
     2461 cccccggcgt agacgcccac gagggcgcgg gccgggaggg cgaaaaccag gaccgaagcg
     2521 acgaccggga tgatcaagcg gcgcttcatg ggaacctcct tttccgatcc tgatcccggc
     2581 gggcggcgcc ctccggtccg tcttttattc ttgcggactc ccgccgccgt gtcaagaaca
     2641 tgtctccggg ttttccggcc ggccgcgcaa ataatccgta tcgcgcgcgg aatccgagga
     2701 atgaaaccgg cgttttctcg acatgaggac aagccgcgtg aatgagaccg cgatcgggga
     2761 aacaggggga atcggccttg tgtcccccct ttgccgaacc ggcccgcgga ggcggccgtc
     2821 aagcctttga caaacctttg ccccggcgat ccgtatgaac gagcgtgtcc ttgtggagac
     2881 aaacggtcga acatggctaa aatcaacttc ggcgaatccc tttccctggc cttttcctcc
     2941 ctctgggaga gcaagctccg gacgttcctg accctcctgg gcatcatcat cggcgtcctg
     3001 acgatcatca ccgtggtctc cgtcatccag ggattgaaca attatgtcta cacgaagatg
     3061 gcctttttcg gggcgaacga tttctccgtc tcgaaatttt ccagcttcgc ctcgaccgtc
     3121 aaggaatacc gggaacagat gaagcgccgg gaccttaccc tggaggacgt ccgccttctc
     3181 cgggaaaaag cctcggcctg cgagctgatc ggcgcctcgg accaagtccg ccggacggtg
     3241 aaattcggaa gcgccgagat ccaaaacgcc gggatcatcg gcgtcacggc cgtcgatcac
     3301 ctgatcggct cggtcatcga gctggagagc ggccgccacc tcctccagga ggaggaggac
     3361 cggtcccggt tctcggccgt gatcgggtcc gacgtggccg aaaagctttt cccccgcctc
     3421 gatcccgtcg ggcggacgat caaggtcggg gccgatcgtt tccggattgt cggcgtgggg
     3481 aaaaagctcg ggaagatcct gggcatgagc cgggacaact atgtccgcgt cccgatcacc
     3541 accttcctca agacattcgg ctcgcgccgc tcgctctcca tcaacatcca cacgtcctcg
     3601 cccgaggcca tggcccgggc ccaggacgaa gtccggacga tcctccggag caagcggaag
     3661 ctgtcctatg acaagcccga tgatttttcc ttccaaacgt ccgaaacgtt catccagttc
     3721 tacaagacgg ccaccaacgg gatttacttc gccatgatcg gcatcgcctc gatcgccctg
     3781 ctcgtcgggg ggatcgtggt catgaacatc atgctggtgg ccgtgaccga acggaccaag
     3841 gagatcggga tccggatggc catcggagcc cgccgcaagg acatcctcgt ccagttcctg
     3901 atcgagtcgt cggccatcgc cggggtcggc ggattgatcg gaatcgtcct gggcgtcgcc
     3961 gcggcgaaaa tcatctcggc ggccacgtcc cttccctcgg cggtcgatcc cgggtccgtc
     4021 atcgccggga tcgtcatgtc agcgaccctg ggcctgtttt tcggcatcta cccggccaac
     4081 aaggcggcca agctcgaccc ggtcgtggcc ttgcggagcg aaacatgagg ctgggcatct
     4141 tccgggaaat cgtcgcgatg gccttcgatt ccattcggac ccacaagctc cgctccttcc
     4201 tgaccctcct ggggatcatg atcggggtga tgaccgtcat cgggatggtc tccatcatcc
     4261 agggactcaa cgcctccttc ctccaggaac tgcaatcggc cggatccgac ctgatcatcg
     4321 tccacaagta cgacccggtc gccatgcgcg aacggacggc cgaggagcgc gcccgccctg
     4381 acctgaccta cgacgacgcc cgggcgatcg aggaggaatg cgatctggtc aaggccgtct
     4441 ccgtcgccct cgccccgtcg tttctcgacc cgatcgagat caagtaccaa aaccagaaaa
     4501 gcgaggggtc ggtcgttctc gggatcaacg accgcttccc ggccgtttac aacatctacg
     4561 cccccaagga gggccggttc ctgacggcgt ccgaaatcgc ccgcagcgcc cggacctgcg
     4621 tcctgggatc cgagctggcc gacatcctct tcccccatca aagccccctg gggaagacga
     4681 tccggatcgg tccggggaat ttcaccgtgg ccggcgtcct cgccaagcgc ggtcagatgt
     4741 tcggccagag ccgggacaac atcgtcgcca tcccgatcac gactctgatg aagcattttc
     4801 cctacgatcc cgagtcgatc gagatcggga tcgttccccg caagccggga acgattcaag
     4861 agacgatcga acagatccgg gccaccctcc gcctccggcg gaaagtgccc ttcggccggc
     4921 ccgataattt ctccctcttc acccaagaca cgatgctcga cttgtataat caaatcaccg
     4981 gggcggccta cctggtgatg atcatcatct cctcgatcgg cctgctcgtc ggcggcatcg
     5041 gggtcatgaa catcatgctg gtcgcggcca aggagcggac ccgggaaatc ggcatccgga
     5101 aagccctcgg ggcccagtcc tccgacatcc tccgccagtt cctggtcgaa tcgatgttcc
     5161 tgaccggaac gggcgggatc ctgggcatgc tcgtgggatt cgccgtggcc ctgatcgtcc
     5221 gggcggcgac accgctgccg gccgccgtga ccggctggtc gatttttctg ggcttgttcg
     5281 tctcgatttc cgtcgggctc ttcttcggga tcttcccggc ccagaaagcc gcccgcgtcg
     5341 atcccatcgt ctccctccgc tacgaataaa ttaagacgac accttgccaa ttcaccttat
     5401 ttctccgttt tttccggccg gccgcgcaaa atatccgtat cgcgcccggc atccgaggaa
     5461 tgaaaccggc gttatctcga catgaggaag agccgcgtga atgagacagc gatcggggaa
     5521 ataaggtgaa tgggtattat gtctcctatt ttttggctga tcgggagaaa gggaaaattg
     5581 cgttaattgg gattgtgtct cccgatttct tcggcgacgg cggaggaggc ccgggcgacc
     5641 aaggcgtcca ggtcggcctc ctgatcgccg gcgatctcga tcagcgacgg ccctccccct
     5701 cccttgccgt tgaacagggg cgccaggacg ccggccagcg gccgaaggtc ggccgcgacg
     5761 ccgtccgccc gggccaggac caggcgggct ttcccgccgc tccgggtcgc gaaaacggcg
     5821 gccgccggcc cgagcttgac caagctcaag gccagggccc gggctccctc ggggcttttt
     5881 tcggccaaga cgcgggcgac gatccggccc gacgctcctt ccgccagctc ccgggcttcg
     5941 tacccggccg cggcctgctc gaccgtcttg agccgcttct tcacgtcctt gagctcggcc
     6001 ccctgcttct cgacggccgc cgggatctcc cgatcgggga cgttgaattt cccggcaagc
     6061 ccccggcaga cgcgcgtttt gacctggtaa tcagccaggg cccggccgcc gcagacaaaa
     6121 gtgaagcgga ggtttccccg gatccgctcg gcggcgatga tcttgatgag gccgatctcg
     6181 cccgtcctcc ggacatgcgt cccgccgcag gcggaataat caaagccctc gatctcgacc
     6241 acccggacga ggcccgaaac cttgggcggc cggcgcaagg gaacctcggc gatccgcccg
     6301 gccgggacga aatacgtctt gaccgcccgg tcttcgaaaa cgaccgcatt ggcccgccgc
     6361 tcgacccggt cgagcgtctc gtcggtgata ttctcgatcc cgatttcgag cgtggacgcg
     6421 tccgccccca ggtgaaagga gcgggtttcg ccgttcagga tctcgatgaa ggcctggctg
     6481 aggacgtgct gtccggaatg ctgccgcatg tggtcgaacc gccgcctccc gtcgaccagg
     6541 cccttgacct tctccgcccc ctcccccgct tcctggcccg gctcccgctc caaaacgtgg
     6601 acgatcgtcc cctcctcgtc gaggaccttc acgacgggaa tcccgtcgat cgtccccagg
     6661 tcccagggct ggccgccgga ctcggggtaa aaagccgttt cgtctagaat cacggccggc
     6721 cggccgtcca ccgtccgccg ccccgtgatc cgggcctcga attccagccg gccggcgtcc
     6781 tcgaaataca atcgtttcgt ttcactcatg atcgactccg tctccctttt ccgcggaagc
     6841 cttcccgtct cccctcacaa caacctaaaa acgaccaagg ccaacagggc cgccccgaaa
     6901 accacggcga ccggggggat caaaatctta taaacatcct tcaggtccgc cttgaaataa
     6961 tccgcggtga gggccaggca gagatgggcc ggcgaaagaa gaaccccggc gaacccgctg
     7021 acataagcga acaggaaaag aaccatgtcc gggctctccc ggccgatcac cggcacgagc
     7081 agcggaaagg cgatcccggc gaaagcctgg ttgacgccgg tcaggaggcc gatcgtgaac
     7141 ggagcggcga acaggagaaa gatccccgag gggccggtcg gcgagacgat cgtcatgacc
     7201 gcgtccaagg cccccgacgt ctccaggatc cgcttgaaga ccatgaccgc ggccgtcaaa
     7261 agaatcatcc ggagcgacaa actctccttg accacggccc agcgctcccg ccccggcagc
     7321 ttcacgaaaa aggcgctcag gacgcaggcc gaggccaggg cccagagcat cgggatccga
     7381 aaaatgaaaa tcagaaggat cgtcaggaag atcggccaga tgctgacgaa tgtccggacg
     7441 atgtccttca cccgcggccg ggaggcctgc tcgttccgct cgtaccgcac gtggcggaag
     7501 aggatcgcga ggccggccgc gacggcgatg accgtgaacg gaaactggtt caagcagacc
     7561 ttcacgatcg ggatcccggc gatcgtcgcg gccaggatca ggttgggata catcggccag
     7621 ctgtattccc agatgtggcg gaaccaataa ttcaggaagg ttttccaggc cggggaaagc
     7681 gtccatcgcc gggccgcctc ctcgacgatc ggggcgccca tcatcgcccc ggccgtcatc
     7741 ggcagaaggc cgatgaaggc cgaggggatg gccaggatga ggcgggcgtc gcggacgtaa
     7801 ttcttcaggg cggcgaccat ctccttgaaa tggccgccgg cctggagaaa acggccgagg
     7861 tagagaacca ggatgagaat gccgatcaag ttcagggttt ccggatcgat cagggcggct
     7921 cccgcctgtt tgacgaacgc ggccgccggc atggcgaaaa gaacgcccgt caggatcgcg
     7981 tccaggacaa ggacgatccc caagtcgacc ttgagcttga cgagaaacaa cagggcgaaa
     8041 atgacggcga ggatcttgag aaggacgatc atgagccgag ggccttgccc gtcgtccgga
     8101 agtgaacctt ggcgaccttg gccaggcttt cccggccgtg gcggcggacg aactcggccg
     8161 cggccgcttc aaccgccgac gaggcgccct tggggaattc catgccccaa tcccggccga
     8221 gaaaatacag ctttttgagg aactcggccc gggccaggat cgaggccgcg gccaccgccg
     8281 ggtcggattc cccccggacc atctgggtga gggtcacggt cttgccccgg gccaggaggg
     8341 cgttgcggac ataccgctcg tcgccgaact tgtcggtcac ggcccgccgg cagtccatct
     8401 tttccatgat gttttcgatg gcccgggcgt gcccccaggc cagcagccgg ttcaagttgc
     8461 gaagcttctc gtagagctcg ttgtaccgct ccgggccgat cgccacgacg gcgtggggat
     8521 aaccccgctg gagcgtctcg gcgatctccc ggcaccggtt gtcgctgatc gccttgctgt
     8581 cgcggacgcc cagctcccgg aggacgcctt cctggtccgg cggcaggtag aatccggcca
     8641 cgacgagggg gccgaagtaa tcccccttgc ccgactcatc ggtgccgatg cggccgggct
     8701 cggccggtat caaatcttgt tgtttcatgc ggcgtccgaa accggaagaa ccggcggacg
     8761 attttacccc gctcccgtca ggatttcaaa taggcgtcga tcgaggcggc cgccgtccgg
     8821 gcgtcgccca tggccaggat gaccgtcgct ccgccgcgga tgatgtcccc gccggcgtac
     8881 acgcccggca ggctggaggc catcgtcgcc ggatcgacct cgatcgtccc ccacttgccg
     8941 accttgaggc cgggcatcgt ctccagaagc agcgggttgg ggctctggcc gatggccacg
     9001 acgaccaggt cgaccggggc cgtgaattcc gagcccggga cgggaacggg ccgccgccgt
     9061 cccgaggcgt cgggctcgcc cagctccatc cggaggcagg tcatggacgc gagccgccca
     9121 taggcgtccc cctcgtagcg gatcgggacg gtcaggaaat ggaactcgat gccctcctcc
     9181 tcggcatggg cgacctcttc ctcccgggcg ggcatctccc ggcgggagcg gcggtagatg
     9241 atcatcgccc gctccgcccc cagccgtttc gccgtccgga cgctgtccat ggccacgttc
     9301 ccgccgccga tcacggccgc gttcttcgcc cggaagacgg gcgtatcgta ggccggaaaa
     9361 tcgtaggccc gcatcaggtt gacccgggtc aggtattcgt tggccgaata cacgccgacc
     9421 aggttctcgc cggggatctt catgaaattg ggaaggccgg cccccgtccc gatgaaaaag
     9481 gcctcgtatc cgcgggcctt gaggtcatcg atcgcggccg tcttgccggc gacgaagttg
     9541 tacctgatct ccacgccgag ccgccggagg tattccacct cggccttgag aatggccttg
     9601 ggcaatcgga actcggggat gccgtagatc aaaacgcctc cgggctcgtg gagggcctcg
     9661 aaaatcgtca ccgcgtggcc cttcttagcc agctcgccgg cggccgtgag gcccgccggc
     9721 ccggccccga tgaccgcgac cttccggccg gtcggaggcg gaagctccgg gagggcgacc
     9781 tcgccggccg cccgctcgta atcggcgacg aaccgctcga ggttgccgat ggccaccggg
     9841 acgccggccg ctcttttcag gttgcagacg gcctcgcact ggctttcctg cggacaaacc
     9901 cggccgcaga tggccggaag agcgttggtt tccttgatct tgaatgcggc ggcgatgaat
     9961 tcgcctttct ccaccagttt gatgaacgag gggatgtcga tcgaaaccgg gcagcccgag
    10021 acgcatcccg gcttggcgca gtcgaggcag cgccgggcct cggccgcggc cgcctcgggc
    10081 gaaaggccgc ggttgacctc gagaaaatcg cggccgcgct cgcccgcccc gcgctggggc
    10141 atgggctgcc gggggagttt catccgctcc ttgaccggcg ggttccggcg gagctcggcc
    10201 ctccaatcct tgacatattc cgccttcagg cgcgcggcca gctcgggcgg aagctcgatc
    10261 ggtttctcgg ccatgatgaa cgcccgccct agggcaggtt ctccttttcc cagcggcaga
    10321 gcgcctggat ttcctcgtcg aaatacgatt tccgccggag gaagagctcg tcccagtcca
    10381 cctcgtggcc gtcgaagtcg ggtccgtcga cgcaggcgaa cttcgtcttc ccggcggccg
    10441 tgcagcggca ggcgccgcac atccccgtgc cgtcgaccat gatcgggttg aggctgacga
    10501 tcgtcttgat gccggccggc cgggtcgcct cggagaccga gcgcatgaag gccgtgcagc
    10561 cgacggccat cacccggtcg attttttcag ccgcggccag ggccttgatt ttctcggcga
    10621 cgtattcccc gcccccgatc cccggatcgc gggaggaaac gatgaggcgc tcgctcaccc
    10681 tccggtagcg gtcgtcccaa tacacgagat aggaagcctt ggcgtcgagc acggtgatga
    10741 cccggttgcc cgcccggcgg agggcccggg ccgtcggcca gagggccccg attccgtagc
    10801 atccggccgc cagcaccacc gttccgaaat tcgcgatctc ggtcgggcgg ccgagcggac
    10861 cgacgaagac cgggatctcg tcgttggact tgagcagggc gagcttgcgg gtcgccgtcc
    10921 caagcaccag gaagaccgag gtcacggtcc cccgggcggc gttccaatcg gcgatcgaga
    10981 gcggaatccg ctctccccgg gcgtcgggca tgaggatgac gaactggccc ggcttggccg
    11041 cccgggcggt atcgggcgcc tcgacctcga tcaaatgcag gttgggcacg agccggcgct
    11101 gggcgatgat ccggggcatt tatcgtctcc gggccgtccg cagggacttg atttccaaag
    11161 ccagggggaa tcccaggcag tcgtcgggat cgccgcgttc gccggcggcc agcggatcaa
    11221 actccgtcaa gcggtcggga atcgcccgga atcccatctc ggcgaacgaa gctccgccga
    11281 tgaaaagcgg ccgggcctct cctccgctcc ggcgggactt ggcgaagaag gccgtcgcgg
    11341 ccggggcggc gatctcggcc cccagccgcc gggccagctc tccggcgatc ccgcgggccg
    11401 gccgggcttc gccggccggg gccaggaccg cctccgaaaa ttggatccgg ccctcgtcgt
    11461 tgacgtatgt cccggcctgt tcgggaaaca gggttcgagg aagaaccaca tcggcttggg
    11521 cggccgcctt gccttggaaa gcgcccagga cgacgacgaa ttcctccccg tcgcggacga
    11581 acgcccgagc cggcccggcc gaaaggaggc ctcgcggcct cggggacgcg gagccggcgg
    11641 acgcgccgaa atcggccatg atctcgaccg ccccgcgggc gttggcttcc cagcccgcgg
    11701 gaacatataa acctccggtc ataccggcca ggttccacac ggcggccgcg ttccgcgggc
    11761 cccacggccc ggcgacgaac gcgggaccga agacaaaggc ggccgggtgc cgccgttcga
    11821 gaagagcgat cagcttttcg gtcttggccg agaccgtctc cgcccgggac cagaccggga
    11881 gctcccggag cgatcggcgg agctcctcga gtcccggagc cccggccggg acgcgtcccg
    11941 aacggttttc gcccacggac cggaggacct cgagaaggaa ccggccggcg tcggccggac
    12001 ccagcttgat tttcagggac gcgcaccggt cgaggcagga gtcgccgtcc cccccgacga
    12061 gaacgagctt cgccccgccc aggacggccc ggttgacggc cgttcccaaa atcggggcgg
    12121 tcagggggag ggcttcctcg aaaatcacga tgagacgggc cttggccagg tcgtcgaagg
    12181 agaaggacgg cgccaggatg tcccctcccg gccggccggc cggaaagccg gaccggtaaa
    12241 ccgaaatatc ctcggccaga tggagggacc gggcgccgag aacgtccagg gaaagccgcc
    12301 ccagcgacca aaggtcctcg caggaatcct gggccgaaag gacggcggcg atgtctccct
    12361 taaggccgcc caggccggcg gccgccgaag ccagggcgtc ctcccagctc gtttcgacga
    12421 gaacgccgcc gcggcggacc atcggccgga ggaggcgttt gggatggccg agcaggtcgc
    12481 cgatgatgaa ccggcccttg acgcaggcct gtccgtcgga gggcgggccg tcctgcggcc
    12541 ggacgtcgac cggccggccg tcgcggagac ggaggccgag gcggcagccc tggccgcaga
    12601 gcccgcagac gaacgtccgg gtctcgttga atttcgcccc gtagcggacg gccctttcgg
    12661 ccagagcccc cgtcgggcag acgtcgacgc aggccccgca gaaccggcag ccgacgtcga
    12721 gcaggcgctt gcccatcgag gtcccgaccg agaccggggc gccccggccg acgaaggcga
    12781 tcgcctcggc cccccggatc tcccggcaga cccggacgca ccggccgcag aggatgcaga
    12841 gcccgtcgtc ccggtcgatg aacgggtcgt cccgcaggac gccggtgccg cgataggcca
    12901 tcgggtggga gaggcgcttc aagccgacct cggccacgac cttctggagc tcgcagcgct
    12961 cgtcggccgg gcaaaggatg cagccggtcg gctcgtccgt tttccggctc gtcgccttgc
    13021 tctcctcgca gtcgttcttt tccaagcaga cgaggcaggc cgagggatgc tcggacaaga
    13081 gaaggtcgag gacgtcgcgg cggagatcca aaatccgggg cgaattcgtg acgaccgtca
    13141 tcccctcctc gaccatcgtg gcgcaggccg gggcgggcag ccggcggccg gcgacttcga
    13201 ccaggcagat ccggcaggcg gcatagggaa cgaggcgccg gtgatcgcag agcgtcggga
    13261 tcgaaatccc catcgcccgg gcggcgtcga ggaccgtcgc gcccgcggcg acgtcggccg
    13321 tccggccgtc gatcgtgatc ttcacggccg ctccttcttc ccggccggcc gggcggacgg
    13381 cggggaaacg aggtcgcggc agacgccggc cggacatctt ttttcccgga tatggatttc
    13441 gtattcctcg cggaaatggg ccacggtgga aaggacggga ttggccgccg tctgtcccag
    13501 gccgcagagg gaggccgtct tgaccgtcaa ggccaaggac tccagccggg ccaagtcgcc
    13561 ctcttccgct aggcccgagg tcatccgctt gagaagctcg gacatccggc gcgttccggc
    13621 gcggcagggc acgcacttcc cgcaggattc ctcctccagg aaatggagat aatagcgggc
    13681 caggtcgacg atgcatgtcc ggtcgtccag gacgatcatt ccgcccgagc ccatgatcga
    13741 gccggccttg gccagcgatt cgaaatcgat gcgcaggtcg agggcggagg cggggatgca
    13801 tccgcccgac ggccctccgg tctggacggc tttcaaagtc cggccctcgg ggacgccgcc
    13861 cccgatgtcc tcgatgatct cccgcaggct gatgcccatc gggacctcga ccagcccggt
    13921 gttgcggatc tgcccgacca gggagaagat cttcgtcccc ttgctttttt ccgtcccgac
    13981 ggaggcgaac cgggcgcttc cccggtcgag aacgatcggg acgttggccc acgtctcgac
    14041 gttgttgatg ttcgtcggct ttccccagag gcccgattcg gaggggaacg gcgggcgctg
    14101 gcggggaaag gctttccggc cctcgatcga ggccatcatc gccgtctcct cgccggaaac
    14161 gaacgccccc gccccctgga cgacctgcag gtcgaaatgg aattccgagc cgaggatgtt
    14221 ttgaccgaga agtccgcaaa cccgggcctg gctgatcgcc cgctcgacgt tcttcacagc
    14281 cagcgggtat tcgccccgga cgtagatgaa gccctcggcc gcccccatgg cgtacgcccc
    14341 gaggatcatg ccctcgatga cggcgtgggg attgccctcg agcaggctcc ggtccatgta
    14401 ggccccggga tccccctcgt cggcgttgca gacgacatac ttgggatggc cggaggcccg
    14461 ccggcagagc tcccacttga cggccgtggg gaacccggcc ccgccccggc cccgcaaccc
    14521 ggccgccttc acctcctcga tcacgccctc ggaggtcata tcgaaaaggc ccttggccag
    14581 gccgcggtat ccgccgatcc ggatgtaatc ctcgatccgc tccgggtcga ccttgagatt
    14641 ggcttccgag acgagccgga gctgcttctt ataaaaagga atctggtcca tccgggcgac
    14701 ccggcgcccg gtggacggga tttcgaacgt cagccgttcg acgaccttgc cggccaagcc
    14761 cgtctgcttg acgatcgccg cggcgtcggc cggcttgagc cggccgtaga aaatcccctc
    14821 gggctggacg accacgctcg gctccatctc gcagaagccg tggcagccgg tgacgagaag
    14881 gccgaccttg tcgccggccc cgaggcggtc gatctcggcc tggagggcgt cgcggatttc
    14941 gagggccccg cccgcccggc cgcaggtccc ggcggtcacg acgatcgtcc gacggaggga
    15001 cgtcgtctcc tcgaggaggc gcttccgcca atcctcgaga tggccgggat gctggaggcg
    15061 ggtcatgccg gcttctcccc cgtccggatc cggccgacga tgtcccgggc ggcggccggc
    15121 gtgatccggg catgggtgac gccgtcgagc accaagaccg gcccgatcgc gcagcagccg
    15181 aggcagttga ccgtttccag ggtgaacatc ccgtcggggg tcgacttgcc ggccgagacg
    15241 ccgaggacct tctccagctc ctcgacgatg cggtcgccgc cccggacatg gcaggccgtc
    15301 cccatgcaga cgctgatcgt gtgggcgtcc ttgggccgca ggctgaaggc tttataaaac
    15361 gagagcacgc cgtacagctc gctttcggag acgcggaggc gccgggcgat aactggaagg
    15421 gcggccggcg ggatgtggtc gaaatgacgc tggacttcgt gaaggacggg gatcgtgtcg
    15481 cgctgacggg ttccgaagtc cttcagcagc tcgtcaagtt cgcttttcca gtctcgatcc
    15541 atgaggtccg tcctgaattt tagccgctat cttaccaaat atccgaaagg gagacaacgc
    15601 ggcttcggcc ggcctcacgc ctcgaagacc gtccgtccgc cgaggatcgt ccgaaggacg
    15661 cgcgcctccc ggacgcgctc cgggggaacc cggaaaagat ccatgtccag gacggtcaaa
    15721 tcggcccagg cgccggggac cagccgcccc ttgtccctct cggcgaactc ggcgtaggcg
    15781 ccgccggccg tgaacgcccg gaccgcgtcc tccacggaga ttttttgctc cgggatccag
    15841 ccctccggcc gccggccgtc gatcgtcgcc cgggtcgcgg ccgcccaaac tcccagaatc
    15901 ggatcgagcg gggcgaccgg ccaatcggag ccgaaggcca aaacggcgcc ggcctcgaga
    15961 aacgagcgga aggcgaacga ggtccgggct ctttccgggc cgatcttcgc ctcggcccag
    16021 cggccgtcgt cgaccaggtg gtacggctgg acggaggcga tgacgcccag ccgggcgaac
    16081 cgggggaaat cggccggccg gacgtgttgg gcgtgttcca tccggaaccg ccggtcgcgg
    16141 ggccggccgt ccgccgccgt tcgctcgtac aggtcgagga gagccgcgac ggcccggtcg
    16201 ccgatggcgt ggaccgcggt ctggaggccg gccgcgtcgg ccgcccggat ccgccgctcc
    16261 atgaggccct cgggaaacat gccggccgcc ggaaacccgg acgaggatgg atcgtcggcg
    16321 taaggcgcct caaaccaagc cgtctgcgag ccgagcgagc cgtcgacgaa tcccttcagg
    16381 ccgccgaagc ggaggaagcc ggacccgcct tccgcccgga cgtcgacgtc ggcaagccgg
    16441 tcgatcatcg cgatcggaag atagaagcgc acccggaccg tcaaccgtcc ctcccggaga
    16501 agatcccggc atgcgccgag gctctcctcg ccggcgacgt cgtggaccga ggtgaggccc
    16561 cgggcggccg cggctcggag ggccgcttcc accgcccgct tcttctgggc ggacgacggc
    16621 tcggggatca cctcccggat caagtcacag gccgcgtcct tgagaatgcc ggtgggctcg
    16681 ccggtcgccg gatccctggc gatctccccg ccgggcggat cggtagtcgt ccggtcgatg
    16741 ccggcccgcc ggagggcggc cgtgttgacc aaaacggtgt gcaggtccat ccggtcgagg
    16801 cagacgggat ggtccggggt gacggcgtcg atccattccc ggcggggcat ttcgacgggc
    16861 tggaattttt ggttgtccca cgtcccggcc gaaatccact cccccggcgg gacgtcccgg
    16921 acccgggccg cgacgcgggc gacgaattcc tccttggagg cggcctcgcg cagatccggt
    16981 ccgagaaggc cgaatccccc cgcggccaag tgaacatggc tgtcgatgaa gccgggcagg
    17041 accagggcgc cttcggcatc gatgatttcg gcgccgcggg aagcggcgcg tttcacctcg
    17101 gcgttcgaac cgacaagcag gatccggtcc ccgcgcaggg ccaccgcttc ggccgctggc
    17161 ttggccggat caccggtccg gacgttcgca ttgatgatga tcgaatcggc catggcgcct
    17221 cctgaccggc aattcgcgcc gaacgccgca atactcgcga cgagcagccc gaagagaagc
    17281 cggcgtccgt tcatgcgttc tcccgccttc cattgtatac cctggtgtcc gtcggcccgg
    17341 cccgctcccc gcgcccgcgg gcctcttttc cggcgttttc cggctgttga caagccgacg
    17401 ggaagtggtt aaagatgata aatggaaaag ggccaagcca tgaaatccgt tttcaagagc
    17461 gatccggaca agaaaaggat cgccgcgctt ttcgacgggg ttttcgccgt ggccatgacg
    17521 atcctggttt tggagctgaa gctgccgcgg cttcaagacg gggattcgat cggcgagctc
    17581 tgggcggccc tgcggaatca cggctcgacc tttttcagct atctgttgag ctttttcatc
    17641 ctaggcagcc tgtggatcgt ccacagccgg attttcggga agcttctgcg gatctccggg
    17701 gccgccctgg ccgtccatat ggcgctgttg atcaccgcgg cgttttttcc cttcagcgtt
    17761 catttgtacg gaagctacac cgggaacacg ctggggggat tgatctattt cggaacaatc
    17821 attctcctct acgcggaaat gctggccctg gttatcgtcg ccgaccgcgg gcgtctcttt
    17881 gagcccggcg tgccccggaa cgatgtcaaa aaactccgcc gcggattcct ctggacgatt
    17941 ctgctgctcg ccgcggcgta catccttatc tatcttttcc ccttctgatc tcttttttaa
    18001 atccgattga caggccggcc ccgatgggct aaaagaacgg catggtgaat ccgactctgc
    18061 gcgtatcctc gcccggacgc atctgccttt tgggcgaaca ccaggattat ttcgggctgg
    18121 cgatcatcgc cgcggccatc aacctccgaa tctcgttctc cggacgcccc cggagcgacc
    18181 ggcggatcgt cctcgacctg ccggacatgg gcgaacggga ggaattcgtt ctcgacggcg
    18241 gccaaatccc ctacgtgaag aagcgggatt acctgaaaag cgcggtcaac atccacctcc
    18301 gggccggcct gccgatgtcc ggctgggacg tcgaggtccg gggcgacatc cccatcaacg
    18361 ccgggacgtc gagctcctcg gccatggtcg tcgcctggaa caagtttctg ctcgaggccg
    18421 ccggagatcc ccgggcctcc gatccccggg agctggccga gctcggatac gagaccgagg
    18481 tggccgaatt caaggaaccc ggcggccgga tggatcacta tgcctcggcc cacggcggcg
    18541 tcgtctggat tcgtttcgag gagcaacttg ttccaggcg
//
LOCUS       DAOOXH010000047        18291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_2933_length_18291_cov_8.717975, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000047 DAOOXH010000000
VERSION     DAOOXH010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 18291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18291
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2933_length_18291_cov_8.717975"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PKV64_06850"
     CDS             complement(<1..867)
                     /locus_tag="PKV64_06850"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPN16835.1"
                     /translation="MFRFRARLQAFAVLALMVAALGMLVPPAAAAQNSSAPERFFDTA
                     RAPKDLVRLVIFYPAPNTFRHLLALKAEGMLPIDNLEIVGVYHVKERTDYRESVRFVR
                     DNKLANVHFHAVSAELGIASLYQANAASAEFRKIFDLSDGIIFFGGPDMPPAAYGEET
                     DFRTVVENPYRHYLELSMIFHLLGGGRNPDFKAFLEDRPDYPVLGICLGMQSLWVGTG
                     GTMIQDLWSEIYGKTTVEDVLALPRDEWHKNPYEMIAPHDPALAPYMLHPVRLLPESR
                     VWKAMGPALDGYP"
     gene            1393..4734
                     /gene="mfd"
                     /locus_tag="PKV64_06855"
     CDS             1393..4734
                     /gene="mfd"
                     /locus_tag="PKV64_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPN16836.1"
                     /translation="MSLDFLKETPEFKDLVRAVSALPKTPEGQALLFGEEDRPRLSIS
                     GLTEAAVPYLFVQLALTGPRRVVFIRPSARPLSGFEDQARFFLQNLGSTKTVRSFPAL
                     SENPAPDAAPSLESAATRMRYFYDLLNRPPALTVTNLFGLLRPFPAPECLPELFLRLN
                     KDAECGRDRVIERLASFGYAREDLIAFRGEYAYRGGIVDVFSPWQKNPVRIEFGGDRV
                     VSLREFDPSTQRAVRQTEAALIPSLSEGTAAPAAASVPFTRYLADAVFVFDHPEEIEK
                     EWDEARTDLPSRAGAGPQDGPAGPPFESIYPPALFERLRAEAVYCGELAPAGARREIA
                     FGFQPVPRFDNKIFFFLETMRRIYEERDRCVIFLSTPPMRKKIGALLETKEIPSLEAD
                     SPLPAAAGGEIRLCLGDLERGFSYPRQKIHFFAEKDILTEERIIPARPAARPSITHFQ
                     DLRAGDYIVHADYGVGVFRGLVKMDVEDRAAEFMELGYRDGDTLFVPVEDLSLVQKYT
                     PVGAGQPLLDKLGTGHWLKTKERTKKAVQKLARELLDLYARRKAIKGFAFSPEGRWQD
                     EFEKTFEFEETEDQLRSIKEIKTDMETAAPMDRLLCGDVGYGKTEVAVRAAFKAVMDG
                     KQVAVLCPTTVLASQHYKTFSGRMVLFPVRVAALSRFQPAAERRRIIEDLKNGRVDII
                     IGTHRLLSADVAFRDLGLLVVDEEQRFGVAHKEKIKHLKASVDVLTLTATPIPRTLNL
                     SLTGLRDISLIETPPKDRLAVHTVVTTFSPRLIAAAIRQEIARGGQVYYVLNKIEEMD
                     SLSAKIREWVPDARLAVVHGRMAPAELEMKMIDFINQKYDVLVSTTIIENGIDIPLVN
                     TLIVQRADHFGLAQLYQLRGRVGRSSRQAFAYFLVPPFAELTPIARKRLEAIKEFSEL
                     GSGFRLAMKDLEIRGAGHLFGEQQHGTMEAVGYDYFIHLLEQAIKELKGGEVEEARCE
                     INLRVEVRIPEAYIPAINVRLSLYKRISSAADLDEIRSLEEEIRDRFGPQPPSVEHLL
                     QYGRIKILAQRLKLSAVDRVDRKLILKFQPSTDVDPARISSLLNKMRGSMTPEGVLSL
                     PLRSPASAGVLRETARLLLELSGMG"
     gene            4760..5731
                     /locus_tag="PKV64_06860"
     CDS             4760..5731
                     /locus_tag="PKV64_06860"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPN16837.1"
                     /translation="MKRNPDFLAAAVLAAAALCLGAGACGGKTGSPPASEGFRLDDPQ
                     KAALPVLTVEGIAFTNADFARFARLTVGEPEASLTAEAAGRLFDDFVERKLIVLSAAA
                     RGIDLSDEEKEKSLAVFKLNRAEGAVSSSEADNADLLEGVLVEKYMALEISGITVPED
                     EIAAYYEAHKNEFLQPERLQVSQILLSSEGRASEILEKIRSAGENEFREAARTSSEGP
                     EAKNGGLMGVFSLGQLPLELEKVIFALEEGRISRVVQSPYGYHIFRLDRKFEPRLRSR
                     GETVPLIEARLLEQKNQAARDARVASLKETMSWKIRTENLPFVYPGS"
     gene            5745..6725
                     /locus_tag="PKV64_06865"
     CDS             5745..6725
                     /locus_tag="PKV64_06865"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPN16838.1"
                     /translation="MSKKKIILAVSLLLVAAVAVPARQVIEEIIAVVNDDIITLSDFK
                     TEYEERLMAARAQYRGEELERAIEYTKAHILDEMITDLLLIQMARKMNINVTDQLKMV
                     VENIKRANDIENDEMLKRALAQQGYNYDEWIATMEKQILREAVLRNQIGQKVVIDDAE
                     IVDYYKKHQDEFVVPEEYELRAVYLAIEGREAAALEARKAEIEAKIKGGAPFAEVAEA
                     DSDEPLKAAKGSLGTFKEGELDRTLLAAVKPLNKGGMTPWVQSKNGWYLILVEEKKDS
                     RLRTFDESRGAITEKIGTQKQNAEVDKFLAEIKSKNYIKILKPDPFADLN"
     gene            complement(6718..7506)
                     /locus_tag="PKV64_06870"
     CDS             complement(6718..7506)
                     /locus_tag="PKV64_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16839.1"
                     /translation="MNTEKAEGGGVPPRLPAAGGGVGWPAGRLGKAVGVFIFFTLVAS
                     AVYALIGAVRAPGAAAVSTAASGKVRSDYVLMLLESCLGLLILFLPSMLEKRFRIILP
                     SRMYLLFVVFLYAAIFLGEVRDFYYRIPHWDVILHAFSGIMLGALGFSIVSLLNKSEK
                     VAVTLSPQFVALFAFSFALSLGVIWEIYEFAADGVLGLNMQKFALEDKTSLVGRAALV
                     DTMKDLIVDALAALAVSVFGFLSMKAEKSWIARVALKRKPEAVS"
     gene            complement(7574..8158)
                     /locus_tag="PKV64_06875"
     CDS             complement(7574..8158)
                     /locus_tag="PKV64_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16840.1"
                     /translation="MMRRIFPASGVALAVVAALIWLGQTGCKSYNEQEVHSSLEVVDV
                     STSWTMKEYRQWPNPKLTLVPTVTFKLKNNSSETLRYINVNAIFKEVDSVENLGDSFH
                     TAVRGEGLAPGATSPPITLKSNFGVAGKTLDSFKANPHWKSYYVKLFVQMGGPRHISM
                     GEYPVSRQIDFQEDQAVHQKDESSAVLQEEPAKD"
     gene            8292..10052
                     /gene="speE"
                     /locus_tag="PKV64_06880"
     CDS             8292..10052
                     /gene="speE"
                     /locus_tag="PKV64_06880"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HPN16841.1"
                     /translation="MGLVKRGKDLWFYEDLYSDVTYGFKVKRIVVPETPTGFQKLMIL
                     DTERFGRVLILDGIVQLTEEDEGIYHEWIGLWPLFATPKTPENVLIIGGGDGGVARAV
                     LRHPGVKSVTMAEIDRVVIEQCRTHLPGISAGVWDDPRFRLIIGDGAEVIRKMKGRCD
                     VIIIDSTDPVGPAKSLFDTSFYESVYDALREGGVTIHQTGALLLQPFEAPGSWRQMER
                     IFDDVQVVQFTNVSYLGGPFSLTAGSRGRNVFKAAARNARRNFKAAGIRTSWYSPDIS
                     AVPYPEFQRRLEVDKYGEEIVLDFPLSGRPPSRPRVGKWSRELCQAIGMLPFGDPMVS
                     DPAWRDDDTLVQYIETSAINFRRFGNTASANCFTCARLPRDEAAAFTTGFFGADAAVC
                     WSLPRGVFADIRKVRRDSLIYRSGASGGPAGEIRRPRPAEAAEIFKPSFRLPVETGFA
                     PAFELVMDIFDCDFDRISSCEAVAAWARESARTAGLKTIGRPDAPDFGHAKKKTAGPS
                     VTQFLRGGSNISHYSINWLMIVLNLVAREPVPLRRIITHAMDYFQGKKAHCWILPRGA
                     SGKSLKDIAENTVLFEVRRD"
     gene            10282..10902
                     /locus_tag="PKV64_06885"
     CDS             10282..10902
                     /locus_tag="PKV64_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPN16842.1"
                     /translation="MRKFSAAAAVLPLFLTLAVVPAQAVQITGKGIMLGLSLSRWSGE
                     DIGRHDAWMSFALGGFLTFNIIDELSVQPELLFVQKGTKWPDNDLNLNFRLSYLELPI
                     LAKYSFTVGPGVKVFIFGGPAFAFKVGATMRFEWQGELITDDIKGMKSLDLGLAFGAG
                     GELPIGVTRLTVGLRYTLGLMNCYEVEGEALEAKNGALLIMAGIGF"
     gene            complement(10922..11761)
                     /locus_tag="PKV64_06890"
     CDS             complement(10922..11761)
                     /locus_tag="PKV64_06890"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HPN16843.1"
                     /translation="MKRKRRRPLAWLIPLAFAILTAVFAFLLTRPRPTGTSPAGPPSK
                     SGPVAKKRMPPASSVPLAAIIIDDIGYSLEAVESIYAVGRPLTVAVLPDADLTEESAR
                     LAAACGLEVMLHLPLESNGNKTDPAGSGPAIREGKSRGEIRAEIDRCLARVPGARGLN
                     NHEGSATTEKAELMRPILEILKERGLYFIDSRTTAATVALREAGRIGVRAAARRVFLD
                     AERGDEAFAAGLRELFRSAKENGRAVAICHPKRESLAALARHVGLAESFGVRLVFASA
                     VVE"
     gene            complement(11758..12903)
                     /locus_tag="PKV64_06895"
     CDS             complement(11758..12903)
                     /locus_tag="PKV64_06895"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPN16844.1"
                     /translation="MKLPRPRILVSYGLAAAAFFLLLWVRPPLQSSKLPPAKDFDILD
                     RAIEHIRDDYVNETDPLQTMEGAFRGLIGALDPASAYLSSELTARASSRRLERLYDIG
                     LAAYKRPGAFPVVVAVVEGSPAEKAGIQPGDAVSAVDDRSTLVWSHNEFRFALKSEKP
                     APVKLRLIHENETLEKTVDRYAPYPAPVAWTAEPGTAGIVRIHHLFSGAMDAFRTIVL
                     PQVAGRKNTLILDLRGCREGEIEEARGLVNLFLKSPRAGTFEKKGGIKTVWACPAEPV
                     LPDVPLVVWVDPATMGPAEIAAGVLRDFKRATVVGTPTAGLTAEQRLLPLDAGDGLLL
                     TVGAFTLASGEALFEKGLTPDVKIGPGKKTGADYLEKTKGLPGSRRP"
     gene            complement(12936..14123)
                     /locus_tag="PKV64_06900"
     CDS             complement(12936..14123)
                     /locus_tag="PKV64_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPN16845.1"
                     /translation="MSRRRIVTAVLGGWLVFAPFLARSAFGQSEPAAASGQAVEYEKR
                     LAEIRGDIERLRAKLGDEEKRERTVLSQLDRIAVRKRLLRSEWKLLQVQLARTRADRD
                     AVGRSIPEMRNALAADRDRLARVLLTIYKHGRFSVARYALEARDLRTFVDQIRALEAV
                     AGAQDRVIADFLSRLAALGQADRELGEKEAEIAALTKQSTAKQVELEAEEKKNRTLVA
                     QIKTNQKTYEQAIGELNQRARELEKLLRDLESRPHPGVFPGLPFGETKGRLDWPVDGR
                     VTQNYGLQKGSFNTKTENNGIEITPSPGDLTIRAIHAGKVVYADFFPSYGNLLILEHG
                     GGYHSLYGHCAEFLVRNGDFVGPGTPLAVAGDTASLSGISLYFEIRYQTKPVNPLLWL
                     RRR"
     gene            complement(14120..15061)
                     /locus_tag="PKV64_06905"
     CDS             complement(14120..15061)
                     /locus_tag="PKV64_06905"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPN16846.1"
                     /translation="MRRKTLRFLLRLALGLGGLAAVFFLSRAKLPEIGRALGGADWGL
                     LALSFCLHALGLLISVHRWRILARAQGDDIPFGFLAKSYLIGKFFNTFLPTSFGGDVV
                     RIWDGSKYSSSIVKSSAIVVVERMTGVIVLFFFALAASLGRLEMAGKVPFIGAALVLG
                     TAGLASAAVFFLPLTGRWLRGGAPDGLLRKARDKALLFREAILSYRRRPRPFFRAMLW
                     AVFLQLNVIVYFILIGISLDLPIPAFDYFIFIPLVLLVQMLPVTINGLAVREGAYVAI
                     FAYYGLSSSAALSYAFVDLAYSLVIGAAGAVLYLTRR"
     gene            15332..15865
                     /locus_tag="PKV64_06910"
     CDS             15332..15865
                     /locus_tag="PKV64_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16847.1"
                     /translation="MKRKIMVTVLALAVLAGTAAAGTKEFWKGGIYVTPQIGVNSWGG
                     SIPFGVNAEYGLTENIGIGGSVMLNMWSEEYWSSTLINFNADVAYHFTKLNAEKFDLY
                     AGGGLGYSAYSWKWKDSSGGGIGGTGSSGIYIPIFVGGRYYFNPKMAVSLRLVGSLTG
                     HWSGFGGVLGVTFVLGK"
     gene            15942..16670
                     /locus_tag="PKV64_06915"
     CDS             15942..16670
                     /locus_tag="PKV64_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16848.1"
                     /translation="MKKFIAGMLIIAASALFLSADVYIKSNSHTDAMSMMGQTTPATD
                     TVSELWIGDNALAMVSQDGRTVIDLAKNTFAMINMAEKSYVETALPVDFAKLLPAEMA
                     SMAGMFVMSATVNPTGETKKVGSWNCQGYDMTMTVMGMPMKTKIWASTEVAFDWAKFS
                     ERFMPAMTKGMMRLDDASVKEMMKIKGYQIASEVNAEMMGAKIHSTTTVAEISMKAAP
                     AGIYGIPAGFTKKATLSMDDVQKR"
     gene            16879..17625
                     /gene="rlmB"
                     /locus_tag="PKV64_06920"
     CDS             16879..17625
                     /gene="rlmB"
                     /locus_tag="PKV64_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPN16849.1"
                     /translation="MDQVNRINPVVEILRASPRRINKLFLQKEEGHRPVGTIVGLAKA
                     NRIPCVFLPKQALDKMSPHHQGVVAVLSPKEYVSLEEILASAGPSPFLVLLDEIEDPQ
                     NLGAVLRSAEGAGADGIVLPERRSAGLTETVFEVSAGALEHLRVARVPNLAQTMDALK
                     KRGFWLVGAESGGDGRYFEFDYTVAVAVVLGSEGKGLRPLIRRKCDRVLSIPMRGRVN
                     SLNVASAASVFFFEIARQRSIPSGALRPDR"
     gene            17805..18020
                     /locus_tag="PKV64_06925"
     CDS             17805..18020
                     /locus_tag="PKV64_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013751714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HPN16850.1"
                     /translation="MKTNMGVVDRLVRIAAAAAIVVLYCLEVINGVWAVVLGIVAVVF
                     IVTSFIGFCPLYVPFRISTKKKPKPSP"
BASE COUNT         3569 a         5517 c         5817 g         3388 t
ORIGIN      
        1 gggatagccg tccaaagccg ggcccatggc cttccaaacg cggctttcag gaaggagacg
       61 gacggggtgg agcatgtaag gggccagggc cggatcgtgc ggagcgatca tctcgtaggg
      121 attcttatgc cactcgtccc gggggagggc gaggacgtcc tcgacggtcg ttttcccgta
      181 gatttcgctc cagagatcct gaatcatcgt tccgcccgtg ccgacccaaa ggctctgcat
      241 gccgagacag atgccgagga cggggtaatc cggccggtcc tccaagaagg ccttgaaatc
      301 cgggttccgg ccgccgccca ggagatggaa gatcatcgac agctcgagat aatgacggta
      361 aggattctcg acgacggtcc ggaaatccgt ctcttcgccg taggccgccg gaggcatgtc
      421 cggtccgccg aagaaaatga tcccgtcgga gaggtcgaag attttccgga actcagccga
      481 ggccgcgttc gcttggtaaa gcgaggcgat tcccagctcg gccgaaaccg catggaagtg
      541 gacgttggcc agcttgttgt cccggacgaa acggacggat tcgcggtagt cggtccgctc
      601 cttgacatgg tagacgccga cgatttccag gttgtcgatg ggaagcatcc cctcggcctt
      661 gagggcgaga agatgacgga acgtgttggg ggccgggtag aaaataacca accggacgag
      721 atccttgggg gctcgggccg tgtcgaaaaa gcgttccggg gccgatgaat tttgggcggc
      781 cgcggccggc ggaacgagca ttcccaaggc ggcgaccatc aaagcgagga cggcgaaggc
      841 ctggaggcgg gctcggaatc ggaacatgca ttactccttg cggccggcgt ctccccggaa
      901 atccggcgga atccggcggc cgggaagagc tcattatacg tgcggccgga cggggagaca
      961 atctctctcc cccgcgctcc gtttttcggc ggcgtttcct ccgcgccgac cgacccttcc
     1021 tctttgtttt aatcgagttc ttttcattgc gttcatcccg cgctcgcgaa acgaacgccg
     1081 aaaacaaaac ggcctcccct ttccccgact tccagggaca tgcggtagaa gtccgggtcg
     1141 agcgtttctc cgatccatct ggacgagagc gacgcaacgc ttctcccgag atcggcgtcc
     1201 gtttttgccg tcgttccctt ttggaggata atctcgagga ggggggcggg gcaaacgccg
     1261 gttccgtccg cccgggcttc gccgggaacg cgagggtcaa aaagacggaa aatcatgaaa
     1321 catgcttgag gcggtcgagc ggcgggacgc gggaattgcg gcccggattc gcgtataatc
     1381 ttcgttcgtc ccatgagcct ggattttctg aaggagacgc cggaattcaa ggacctcgtc
     1441 cgggcggttt cggcccttcc gaaaaccccc gagggccagg cccttctttt cggagaagag
     1501 gaccggccgc gtctttcgat ttccggcttg acggaggcgg ccgtccccta ccttttcgtc
     1561 cagttggccc tgactgggcc gcgccgggtt gtcttcatcc gtccgtccgc ccgtccgttg
     1621 tccggcttcg aggatcaggc ccgttttttc ctccagaacc tcggctcgac gaaaaccgtc
     1681 cggagctttc ccgcgctctc ggagaatccc gccccggacg ccgccccgtc cctggagtcg
     1741 gcggccaccc ggatgcgcta tttttatgat ctgctgaatc gcccgccggc cctgaccgtg
     1801 accaatcttt tcgggctcct gcggccgttt ccggctccgg aatgcctccc tgagcttttt
     1861 ctccgcctga acaaggacgc cgagtgcggc cgggaccggg tgatcgagcg gctggcctct
     1921 ttcgggtatg cgcgcgagga tttgatcgcc ttccgcgggg agtacgccta tcggggcgga
     1981 atcgtcgatg tcttctcccc ctggcagaaa aatccggtcc ggatcgaatt cggcggcgac
     2041 cgcgtcgttt ccctccgcga gtttgatccg tccacccagc gggccgtccg ccagaccgag
     2101 gcggctctca tcccctcgct ttcggagggg accgccgctc cggccgccgc ctccgttccg
     2161 ttcacccggt acctcgccga cgcggtgttc gtcttcgacc atccggaaga gatcgagaag
     2221 gagtgggacg aggcgagaac cgacctcccc tcgcgggccg gggccgggcc gcaggacggc
     2281 cccgcaggtc cgcccttcga atcgatctat cctccggccc tgttcgaacg gctccgcgcc
     2341 gaggcggttt attgcgggga gctcgcaccg gccggagccc gccgggaaat cgccttcggc
     2401 tttcagcccg tgcctcgttt cgacaacaag atcttcttct tccttgagac gatgagacgg
     2461 atttacgagg agcgcgaccg ctgcgtcatc tttttatcca cgccgccgat gcggaagaag
     2521 atcggggccc ttctcgaaac caaggagatc ccctcgctcg aagcggattc gcctcttccg
     2581 gcggccgccg gcggcgagat ccgtctctgc ctgggcgatc tcgagcgggg cttcagctat
     2641 ccgcggcaaa agatccattt cttcgccgag aaggacattc tcaccgagga gcggatcatc
     2701 cccgcccggc ccgcggcccg gccctcgatc acccattttc aggacctgag ggccggagac
     2761 tatatcgtcc atgccgatta tggcgtgggc gtgttccggg gcctggtcaa aatggacgtg
     2821 gaggaccggg cggcggaatt catggagctc ggctaccggg acggagacac gttgttcgtc
     2881 ccggtcgagg acttgagcct cgttcagaaa tatacgcccg tcggagccgg ccaaccgctg
     2941 ctggacaagc tcggaaccgg ccattggctg aaaaccaagg agcggacgaa aaaagccgtc
     3001 cagaagctgg cccgggagct cctggatctt tatgcccggc gcaaagccat caagggattc
     3061 gctttcagcc cggagggccg ctggcaggac gaatttgaaa aaaccttcga gttcgaggaa
     3121 accgaggacc agctccgctc cataaaggag atcaagacgg atatggagac ggccgcgccc
     3181 atggaccggt tgctctgcgg cgacgtcggc tacggaaaga cggaggtcgc cgtccgggcc
     3241 gccttcaagg cggtcatgga cggcaagcag gtcgccgtcc tttgtcccac gaccgtcctg
     3301 gccagccagc attacaagac gttctccggg cggatggttc tgttcccggt ccgggtcgcc
     3361 gccctgtccc ggttccaacc ggccgccgaa cggcgccgga tcatcgagga cctgaagaac
     3421 ggccgcgtcg acatcatcat cggaacgcac cgtctccttt cggctgacgt ggctttccgc
     3481 gatctgggac tgcttgttgt cgacgaggag cagcggtttg gagtcgccca caaggaaaaa
     3541 atcaagcatc tcaaggccag cgtcgacgtc ttgacgctga cggccacgcc gatcccccgg
     3601 acccttaacc tgtccctgac cggcctccgc gatatcagcc tgatcgaaac gccgcccaag
     3661 gaccggctgg ccgttcacac ggtcgtgacg acgttttctc cgaggctgat cgcggccgcg
     3721 atccggcagg agatcgcccg cggggggcag gtctattacg tcctcaacaa aatcgaggag
     3781 atggattcct tgtcggcgaa gatccgggaa tgggtcccgg acgcccgctt ggccgtggtc
     3841 cacggccgga tggctccggc cgagctcgaa atgaagatga ttgatttcat caaccagaag
     3901 tacgatgtcc tggtctcgac gacgatcatc gaaaacggaa tcgacatccc cctggtcaac
     3961 accctgatcg tccagcgggc cgatcatttc ggcttggccc agctctacca gctgcgcggc
     4021 cgggtcggac ggtcttcccg ccaggccttc gcctattttc tcgttccgcc gttcgccgag
     4081 ctgactccga tcgcccgcaa gcgcctcgaa gccatcaagg aattcagcga gctcggatcc
     4141 ggattccgtc tggcgatgaa ggatctggaa atccggggcg ccggccatct gttcggagag
     4201 cagcaacatg ggacgatgga agccgttgga tacgactact tcatccatct cctggagcag
     4261 gcgatcaagg agctcaaggg cggggaggtc gaggaggccc ggtgcgagat caatcttcgg
     4321 gtcgaggtcc gaatccccga ggcctacatc ccggccatca acgtgcggct cagcttgtat
     4381 aagcggattt cctcggccgc ggacctggat gagatccggt cgctggagga ggaaatccgg
     4441 gaccgcttcg gcccgcagcc gccgagcgtc gaacatctcc tccaatacgg ccggatcaag
     4501 attctggccc agcgtctgaa gctttcggcc gtcgatcgcg tcgaccggaa gctgattctc
     4561 aaattccaac cttcaacgga tgttgatcca gcccggattt caagtcttct caacaagatg
     4621 cggggatcaa tgactccgga aggcgttctc agcctgccgc tccgctcccc cgcctcagcc
     4681 ggcgttctgc gtgaaaccgc ccggctcttg ctggagttgt cagggatggg ataaaataac
     4741 tcattggcat ttggcacaga tgaagcgcaa tccggacttt ttggcggcgg ctgttttggc
     4801 ggcggcggct ctctgcctgg gggcgggagc ctgcggcggc aagaccggat ctcctcccgc
     4861 ttccgaggga ttccgtctgg acgatccgca aaaagccgct cttccggtct tgacggtcga
     4921 gggaattgcc ttcaccaatg ctgatttcgc ccggttcgcc cgcttgacgg tcggcgagcc
     4981 cgaggcctcc ttgacggccg aggcggccgg ccggctgttc gatgatttcg tcgaaaggaa
     5041 gctgatcgtc ctctcggcgg ccgcccgcgg gatcgacttg agcgacgagg aaaaagagaa
     5101 atccctggcg gttttcaagc tgaaccgggc cgagggagcc gtttcgtcct ccgaggccga
     5161 caacgcggat ctcctggaag gcgtcctcgt tgagaagtac atggccttgg aaatatcggg
     5221 aatcacggtt ccggaggatg agatcgccgc ctattacgaa gcccacaaga acgaattcct
     5281 ccagccggag cgcctccagg tgagccagat tctcctttcc tcggaaggcc gggccagcga
     5341 aatcctggaa aagatccgtt cggccggcga aaacgagttc cgtgaggccg cccggacgtc
     5401 ttcggaagga cccgaggcga aaaacggcgg actgatgggc gttttcagcc tcggccaact
     5461 ccccctggag ctggaaaaag tcatcttcgc cctggaggaa ggccggatca gccgggtggt
     5521 ccagtctccc tatggctacc atatcttccg gctcgaccgg aaattcgagc cccggctccg
     5581 gtctcgaggc gagaccgtcc ctttgatcga agcccgtctt ctcgaacaga aaaatcaagc
     5641 ggcccgggac gcccgcgtcg catccctgaa agaaacgatg tcctggaaaa tccggacgga
     5701 gaatctgccg ttcgtgtatc cgggatcctg ataaggaagg tgccatgtcg aagaagaaaa
     5761 tcatcctggc cgtctctctc cttcttgtcg cggcggtcgc cgtcccggcc cggcaggtca
     5821 tcgaggaaat catcgccgtc gtcaacgacg acatcatcac cctctccgat ttcaagacgg
     5881 aatacgagga gcgtctcatg gccgcccggg cccagtacag gggggaggaa ctggagcggg
     5941 cgatcgaata caccaaggcc cacatcctcg acgaaatgat cacggacctg ttgctcatcc
     6001 agatggcccg caagatgaac atcaacgtca cggaccagtt gaaaatggtc gtcgagaata
     6061 tcaagcgggc caatgatatc gaaaacgatg agatgttgaa gcgggccctg gcccaacagg
     6121 gatacaacta cgacgagtgg atcgccacga tggaaaagca gatcctgcgg gaagccgtcc
     6181 tccgcaatca aatcgggcag aaagtggtga tcgacgacgc cgagattgtc gattattata
     6241 aaaagcacca ggacgaattc gttgttcccg aggaatatga gctccgggcg gtctacttgg
     6301 cgatcgaggg ccgggaggcc gccgccctgg aagctcgcaa ggccgagatc gaagcgaaga
     6361 tcaagggagg cgcgccgttc gcggaggtgg ccgaggccga ttccgacgag ccgctcaaag
     6421 cggccaaggg aagcctgggg acgttcaagg aaggcgagct cgaccggacg ctgctggcgg
     6481 ccgtcaagcc gttgaacaaa ggcgggatga cgccctgggt tcagtccaag aacggctggt
     6541 atttgatcct ggttgaggaa aagaaggaca gccggctccg gacttttgac gaatcccggg
     6601 gggcgatcac cgaaaaaatc ggcacgcaga agcaaaacgc cgaggtggat aagtttctgg
     6661 ccgagatcaa atcgaaaaac tacatcaaga ttctcaagcc cgatccgttc gccgacctca
     6721 actgacggct tccggtttcc gcttgagggc cacccgggcg atccagcttt tttcggcctt
     6781 catcgaaagg aagccgaaga ccgacacggc caaggcggcc agggcgtcga cgatgagatc
     6841 cttcatcgtg tcgacaaggg ccgcccgccc gacgagcgag gttttatcct ccagggcgaa
     6901 cttctgcata ttgagcccga ggacgccgtc ggccgcgaat tcatagatct cccagataac
     6961 gcccagcgaa agcgcgaacg agaaagcgaa cagggcgacg aactgggggc tcagcgtcac
     7021 ggctactttt tcgcttttat tgagaaggct gacgattgaa aacccgagcg ctccgagcat
     7081 gatcccgctg aacgcgtgga ggatgacgtc ccagtgcggg atccggtagt aaaagtcgcg
     7141 gacttccccc aggaagatgg cggcgtagag gaagacaacg aagaggaggt acatccggct
     7201 gggaaggatg atccggaagc gcttctcgag catcgaggga agaaacagga taagaagacc
     7261 gagacaggac tcgaggagca tgagaacata gtcgctgcgg accttcccag aagcggccgt
     7321 ggagacggcg gccgcccccg gagcgcggac cgccccgatc aaggcataga ccgccgaagc
     7381 gacgagagtg aaaaagatga agacgccgac cgcttttccc aagcggccgg ccggccagcc
     7441 gacaccgccc ccggcggcgg gaagccgcgg cggaactcct ccgccttccg ccttctcggt
     7501 gttcatgagg actcccccgc tgccggacgg cggcggagag aaagaatccg gccggcttgt
     7561 ctcctccggg aggctaatcc ttcgccggct cttcctgcag gacggccgac gattcgtcct
     7621 tctggtggac ggcctggtct tcttggaaat cgatctgccg ggacaccgga tattcgccca
     7681 tcgaaatatg gcgcggaccg cccatttgga cgaaaagctt gacgtaatac gacttccaat
     7741 ggggattggc cttgaaagag tcgagggttt taccggccac gccgaaattg gatttcaggg
     7801 tgatcggcgg gctggtcgcg ccgggggcga ggccttcgcc ccggaccgcc gtatgaaagc
     7861 tgtcgcccag gttctcgacc gagtccacct ctttgaagat ggcgttgacg ttgatgtagc
     7921 ggagggtttc ggacgagttg ttcttgagct tgaaggtcac cgtcgggacc agggtcagct
     7981 taggattcgg ccattgccgg tattccttca tcgtccagct ggtgctcacg tcgacgacct
     8041 ccagggacga atggacctcc tgttcgttgt aggatttgca ccccgtctgt cccagccaga
     8101 tcagggccgc gacgaccgcc aaggcgactc cggaagcggg gaagatgcgt ctcatcatgt
     8161 tcctcctgtc ggacctcttg atcataaccc gtccggtggg ggaaagcaag aacggtcccg
     8221 ggtttccttg cgcctcggcg cggtttcgct ataataaaat tccacgaaat gtcgatgaaa
     8281 ggacgaatgt catgggcctg gtgaagcgcg gcaaggatct ctggttttac gaagatctct
     8341 actccgatgt gacctacggg ttcaaggtca agcggatcgt cgtccctgaa acgccgaccg
     8401 gcttccagaa gctgatgatc ctcgacaccg agcggttcgg gcgggtcctg attctggacg
     8461 gaatcgtcca gctgaccgag gaagacgagg gcatttatca tgaatggatc ggcctttggc
     8521 cgctgttcgc cacccccaag actccggaaa acgtcttgat catcggcggc ggcgacggcg
     8581 gcgtcgcccg ggccgtcctc cgccatcccg gagttaaaag cgtgacgatg gccgagatcg
     8641 accgggtcgt catcgagcag tgccggacgc accttccggg gatttccgcc ggagtttggg
     8701 atgatccccg cttccgcctg atcatcggcg acggggccga ggtgatccgg aagatgaaag
     8761 gccggtgcga cgtgatcatc atcgactcga ccgaccccgt cggcccggcc aaaagcctct
     8821 tcgacaccag cttttacgag agcgtctacg acgccctccg cgaaggcggc gtcaccatcc
     8881 atcagaccgg ggctcttctt ctccagccgt ttgaggcgcc gggaagctgg cggcagatgg
     8941 agcggatttt cgacgacgtc caggtcgtcc agttcacgaa cgtcagctac ctgggcgggc
     9001 cgttcagcct gaccgccggg tcccgcggcc ggaacgtttt caaggcggcc gcgcgcaacg
     9061 cccggcgcaa tttcaaggcc gcggggatcc ggaccagctg gtattccccc gacatctcgg
     9121 ccgttccgta tcccgagttc cagaggcgcc tcgaggtcga caagtacggc gaggaaatcg
     9181 tcctggactt ccctctttcc ggccggccgc cgtcccggcc gcgggttgga aagtggagcc
     9241 gggagctttg ccaggcgatc ggcatgcttc ccttcgggga ccccatggtc tccgatcccg
     9301 cctggaggga tgacgacacg ctcgtccagt atatcgagac gagcgccatc aatttccgcc
     9361 ggttcgggaa tacggcctca gccaactgct tcacctgcgc ccggcttccg cgggacgagg
     9421 ccgcggcgtt caccaccgga tttttcgggg ccgatgcggc ggtctgctgg tccctgccgc
     9481 ggggcgtttt cgccgacatc cggaaggtgc gccgcgactc cctgatttac cgctcagggg
     9541 cgtccggggg gccggccggg gagatccgcc gtccgcggcc ggccgaagcg gccgaaatct
     9601 tcaagccctc gttccggctg cccgtcgaga cgggcttcgc cccggccttc gagctcgtca
     9661 tggacatctt cgattgcgat ttcgatcgaa tttcgtcctg cgaggcggtc gccgcttggg
     9721 cccgggaatc ggcccggacg gccggcttga aaaccatcgg aagaccggat gctcctgact
     9781 tcggccacgc caagaagaaa acggccggtc cgtccgtgac ccagttcctc cgcggcggtt
     9841 cgaacatcag ccactattcg atcaactggc tgatgatcgt cttgaacctg gtcgcccggg
     9901 aaccggtccc cctgcggcgg atcatcaccc acgctatgga ttatttccag gggaaaaaag
     9961 cccactgctg gatcctgccg cgaggggcgt ccggcaagag cctcaaggac atcgccgaaa
    10021 acacggtctt gttcgaggtc cggcgggact aggccggcgg gggctctcgg aacgaaagaa
    10081 gggttcggcc gcagcgttct ataaaatcct tgaccgaaga caaatcgaaa aaaccctttt
    10141 ttcaatccag ccggggtaag atttttaaat ggaaggacat atcgcccctc ggctggattt
    10201 tagaaaacgt gatccacggc cttgacgctc gtccctctga ttgatagatt gcttccatca
    10261 tggttttgag gaggagtgaa catgagaaaa ttctcggccg ccgcggccgt tttacccttg
    10321 tttctaaccc tggccgtcgt cccggctcag gcggttcaaa tcacgggcaa ggggattatg
    10381 ctgggtcttt ctctctccag atggagcggc gaggatatcg gaagacatga tgcctggatg
    10441 agcttcgccc tgggcggatt cctgaccttc aatatcatcg acgaattgtc cgttcaaccg
    10501 gagctcttgt tcgttcagaa agggacgaag tggccggaca acgatctcaa tctcaatttc
    10561 cgcttgagct acctggaact gccgattctg gcgaaatatt cttttaccgt cgggccgggc
    10621 gtgaaggtct tcattttcgg cggaccggct ttcgccttca aggtcggagc gaccatgcgt
    10681 tttgaatggc aaggcgagtt gattaccgat gatatcaagg gaatgaagtc tttggacctc
    10741 ggcctggcgt tcggggcggg gggggagttg cccatcggcg tcactagact cacggtcggt
    10801 ctccgttata cgctcggcct gatgaactgc tatgaagtgg agggggaagc cctcgaggcc
    10861 aaaaacggcg cgcttttgat catggccggg atcggcttct gataccggcc ggatcgggtc
    10921 ctcattcgac gaccgcggag gcgaaaacga ggcggacgcc gaaggattcg gccaagccga
    10981 cgtgacgggc cagggcggcg agcgactcgc gcttcggatg gcagatcgcg acggcccgtc
    11041 cattttcctt cgctgagcgg aagagctccc ggagcccggc ggcaaaggcc tcatcgccgc
    11101 gttccgcgtc gagaaaaacc cggcgggcgg cggcccggac tcctatccgg ccggcttccc
    11161 gcagggcgac ggtggccgcg gtcgtccggc tgtcgatgaa ataaagtccc cgctccttga
    11221 ggatctcgag aatcgggcgc atcaactccg ccttttccgt cgtcgccgag ccctcgtgat
    11281 tgttgaggcc gcgggcgccc gggacccggg cgaggcaacg atcaatctcg gcccggatct
    11341 ctccgcggga cttcccctcc cggatcgccg ggccggagcc cgccggatcg gtcttgttcc
    11401 cgttggattc caggggcaga tggagcatga cctccaatcc gcaggcggcg gccaagcggg
    11461 ccgattcctc cgtcaagtcc gcatccggca ggacggccac ggtgagcggg cggccgacgg
    11521 cgtagatgct ttcgaccgcc tccaggctgt aaccgatgtc gtcgatgatg atcgcggcca
    11581 ggggaacgga agacgccggc ggcatccgct ttttcgccac cgggccggat ttggagggag
    11641 ggccggcggg ggatgtccct gtcggccgcg gcctggtcag aaggaaggcg aaaacggccg
    11701 tcagaatagc gaaggcgagc gggatgagcc aggccaaggg ccggcgccgc ttccgcttca
    11761 tgggcgccgg ctgccgggca ggcccttggt tttttcgaga taatccgcgc cggttttttt
    11821 ccccggaccg attttcacgt ccggcgtgag gcctttttca aacagggcct cccccgaggc
    11881 gagggtgaat gcgccgaccg tcagcagcaa tccgtctccg gcgtcgagcg gcaggagcct
    11941 ctgttcggcc gttaagccgg ccgtcggggt tccgacgacg gtcgcccgct tgaagtcgcg
    12001 gaggacgccg gccgcgattt cggccgggcc catcgtcgcc gggtccaccc agacgaccag
    12061 agggacatcg ggcagaacgg gttcggccgg gcaagcccaa acggtcttga tgccgccttt
    12121 tttctcgaac gttccggccc gggggctttt caggaagaga ttgaccaagc cgcgcgcctc
    12181 ctcgatctcg ccttcgcggc agccgcgcag gtcgagaatg agcgtgttct tgcggcccgc
    12241 aacttgagga agaacgatcg tgcggaaggc gtccatcgct ccggaaaaaa gatggtggat
    12301 ccggacgatc cccgccgttc cgggctcggc cgtccaggct acgggagcgg gataaggggc
    12361 gtaccggtcg acggttttct cgagggtttc attttcatgg atcaagcgga gcttgaccgg
    12421 cgcgggcttc tcgctcttca aggcgaagcg gaactcgttg tggctccaga caagggtgga
    12481 gcggtcgtcg accgcgctga ccgcgtctcc cggctggatg ccggctttct ccgcgggcga
    12541 cccctcaacg accgcgacga cgacgggaaa cgcgcccggc cgcttataag cggccaagcc
    12601 gatgtcgtaa agccgctcga ggcggcgaga agacgcccgg gccgtcaatt cggagctcag
    12661 ataggccgag gccgggtcga gggctccgat gagccctcgg aaagcgcctt ccatcgtctg
    12721 aagcgggtcc gtttcattga cataatcgtc gcggatatgc tcgatggccc ggtccaggat
    12781 gtcgaaatcc ttggccggag gaagtttcga ggactgcaag ggcgggcgga cccagaggag
    12841 gaggaagaag gccgcggccg ccaggccata ggaaacaagg atccgggggc ggggtagctt
    12901 catcggggtc gttggctccg taaggagaaa tcctatcacc ttcgccgaag ccaaagcaag
    12961 ggattgacgg gcttggtctg gtagcggatc tcgaaataga gggaaatccc cgagagcgag
    13021 gcggtgtcgc cggcgacggc caggggcgtt ccgggcccga cgaagtcgcc gttgcggacg
    13081 aggaattcgg cgcaatgacc gtagagcgag tggtagccgc cgccgtgctc gaggatgagg
    13141 aggttgccgt agctcgggaa gaaatcggcg tagacgacct ttccggcatg gatggcccgg
    13201 atagtcaaat cccctgggga cggggtgatc tcgatgccgt tgttctcggt tttcgtgttg
    13261 aagctgccct tttgcagtcc gtaattttgg gtgacgcgtc cgtcgaccgg ccagtcgagg
    13321 cggcccttgg tctcgccgaa cggcaggccg gggaaaacgc ctggatgagg acgggattcc
    13381 aggtcgcgga gaagcttttc gagctcccgg gcgcgctggt tgagctcgcc gatggcctgt
    13441 tcgtaggttt tttggttggt tttaatctgg gcgacgagcg tccggttctt cttttcctcg
    13501 gcctcgagct cgacctgctt ggccgtcgac tgcttcgtca aggcggcgat ctcggcctcc
    13561 ttttccccca gctcccggtc ggcttggccc agggcggcca gccgggagag gaaatcggcg
    13621 atgacccggt cctgggctcc ggcgacggct tcgagcgccc ggatctggtc gacgaaggtt
    13681 cgcagatcgc gcgcttccag ggcatagcgg gcgacgctga aacggccgtg cttgtagatg
    13741 gtgaggagaa cccgggccag ccggtcgcgg tcggcggcca gggcgttgcg catttccggg
    13801 atgcttcggc cgacggcgtc ccggtcggcc cgggtccggg cgagctggac ctggaggagc
    13861 ttccattcgc ttcggagaag gcgtttccgg acggcgatcc ggtctagctg ggagagaacg
    13921 gttctttccc gcttttcctc atcgccgagc ttcgcccgga gccgttcgat gtcgccgcgg
    13981 atctcggcca ggcgcttttc gtattcgacg gcttgaccgg aggccgcggc cggctcggat
    14041 tggccgaaag cggaccgggc gaggaagggg gcgaagacaa gccagccgcc caggacggcc
    14101 gtgacgatcc tccggcggct catcggcgcg tcaggtagag gaccgccccc gccgccccga
    14161 tgaccagact gtaggccaag tcgacgaagg cgtaagacag ggcggccgat gaggacagcc
    14221 cgtaataagc aaagatcgcc acataggccc cctcccggac ggccaggccg ttgatcgtga
    14281 ccggaagcat ctggacgagg agaacgaggg ggatgaagat gaaataatcg aacgccggga
    14341 tggggagatc cagggatatt ccgatcagga tgaagtagac gatgacgttc agctggagaa
    14401 acacggccca cagcatcgcc cggaaaaacg gccggggccg gcggcggtag gacaggatcg
    14461 cctcccggaa aagaagcgcc ttgtcccgcg ctttccggag gagtccgtcc ggcgcgcccc
    14521 cgcggagcca ccggccggtg agggggagga agaagacggc cgcggacgcc agcccggccg
    14581 ttccgaggac taaggccgcc ccgatgaacg ggaccttccc ggccatctcc aggcggccga
    14641 gcgaggcggc cagggcgaag aagaagagga cgatgacgcc ggtcatccgt tcgacgacga
    14701 cgatggccga cgacttgacg atcgacgagg aatatttcga tccgtcccag atgcggacga
    14761 cgtcgccgcc gaagctcgtc gggagaaagg tgttgaagaa cttgccgatg aggtaggatt
    14821 tcgccagaaa gccgaacggg atgtcgtcgc cctgggcccg ggccaagatc cgccagcggt
    14881 ggacgctgat gagaagaccc agggcgtgga gacagaacga gagggccagg agcccccagt
    14941 cggcgccgcc gagggcccgg ccgatttcgg gcagcttggc ccgggagaga aagaaaaccg
    15001 cggccaggcc gcccagtccg agggccaggc ggagaagaaa acggagggtt ttccggcgca
    15061 tcgattcttt ctaccataag gcaagcccga aacggaaggg gagggggacg tccggatcga
    15121 ttttattcag agaggattca gccgaaaccg gatcgacccg gccccaaaaa gaagaaaccg
    15181 gcggcgttcc cggagggggc ggcaaggcgg gaaataaggt ggaaaaagag agatatgact
    15241 taaatcatag gaaaaataaa aaattcttga caatcgccct cccggtttct atcatgggac
    15301 tcaagccact atctgattga aaggagaatc catgaaacga aagattatgg tgacggtatt
    15361 agctctggcg gttctggccg ggacggccgc ggccgggaca aaggaatttt ggaagggcgg
    15421 aatctatgtg actccgcaga tcggggtgaa cagctgggga gggagcatcc ccttcggcgt
    15481 gaacgccgaa tacggcctca cagagaacat cgggatcggc ggatcggtca tgctcaacat
    15541 gtggagcgaa gaatactggt cgtccaccct catcaacttc aacgccgacg tcgcctacca
    15601 tttcaccaag ctcaacgcgg aaaagtttga tttgtacgcc ggaggcggcc tcggctactc
    15661 cgcctattcc tggaaatgga aagattcttc cggcggcgga atcggcggca cgggcagctc
    15721 gggcatctat atcccgattt tcgtcggcgg gcggtactat tttaatccga aaatggccgt
    15781 cagcctgcgc ctggtcggat ccctgacggg gcactggtcc ggattcggcg gcgtgctcgg
    15841 ggtgacgttc gtcctgggca agtaagcggt ccggacggat acgaccaaga caccgcgccc
    15901 gcccgcggcg ttccgcggcg cgcgggaaag gaaaaatcgt catgaaaaaa ttcatcgcgg
    15961 gaatgttgat catcgccgcg tccgccctgt tcctgagcgc ggacgtttac atcaagagca
    16021 attcccatac cgacgccatg tcgatgatgg gacagaccac gccggccacc gacaccgtct
    16081 ccgagctgtg gatcggcgac aacgccctgg ccatggtcag ccaggacggc cggacggtga
    16141 tcgacttggc caagaacacc ttcgccatga tcaacatggc cgaaaagtcc tacgtcgaaa
    16201 cggcgcttcc ggttgatttc gccaagctcc tgccggccga aatggcctcg atggccggga
    16261 tgttcgtcat gtcggcgacc gtgaatccga ctggagagac gaaaaaggtc gggtcgtgga
    16321 actgccaggg ctatgacatg accatgacgg tcatgggcat gcccatgaaa accaagattt
    16381 gggcctcgac cgaagtcgcc ttcgactggg cgaagttctc cgaaagattc atgccggcca
    16441 tgacgaaagg gatgatgcgg ctggacgacg cctcggtgaa ggaaatgatg aagatcaagg
    16501 gctaccagat cgcctcggaa gtgaacgccg agatgatggg ggccaagatc cactcgacga
    16561 ccaccgtcgc ggagatctcc atgaaggccg cgccggccgg aatctacggg atcccggcgg
    16621 gcttcaccaa gaaggccacg ctcagcatgg acgacgtcca gaaacggtaa gatttctttc
    16681 ctctgcgttt cttcccgggc gccggaacgg atgttccggc gcccggtttc cccccttcgg
    16741 ccaaatcaga acctgtttaa ccatttcccc atttcccggc cgatcgcgga tccggcatgg
    16801 ccggccgggc gccgaaggaa ggatacggcg ttcccggatt ttccggccgg atcggcgtat
    16861 aatgcggaag gattccccat ggatcaagtc aaccgcatca atcccgtcgt cgagattctc
    16921 cgggcctccc cgcggcggat caacaagctg tttctccaga aagaagaggg ccatcgcccc
    16981 gtcggaacga tcgttggctt ggcgaaggca aaccgcattc cctgcgtctt tctcccaaaa
    17041 caggctctcg acaaaatgtc tccccatcat cagggcgtcg tggcggttct ctctccgaag
    17101 gaatatgttt ccctcgaaga gatcctcgcc tccgccggtc cgtccccatt cctcgtcctt
    17161 ctggatgaga tcgaggatcc ccaaaacctg ggggccgttc tccggagcgc cgagggcgcg
    17221 ggcgccgacg gcatcgtcct gcccgagcgc cgctcggccg gcctgaccga gaccgtcttc
    17281 gaggtgtcgg ccggagccct cgaacacctc cgggtcgccc gcgtcccgaa cctcgcccaa
    17341 accatggacg cgcttaaaaa gcgcggcttt tggcttgtcg gggcggaaag cggcggcgac
    17401 ggccggtatt tcgagttcga ctacaccgtc gccgtggccg tcgtcctcgg ttcggaggga
    17461 aaaggccttc ggccccttat ccgccggaaa tgcgaccggg ttctctccat cccgatgcgg
    17521 gggcgggtta attccctcaa cgtcgcctcg gccgcctcgg tctttttctt cgagatcgcc
    17581 cgccagcggt cgatcccctc gggcgcgctt cgcccggacc gttgatgcga aaaatcaata
    17641 acattccgcg gacggatcgt cgataacgcc gttgtcacag ggcgcggatt ccgatgcctc
    17701 ttcctccgga gccgttccgg cgcccggtct ccgccttgac ctttcctctc tttggaatta
    17761 tcttttaaaa aagggggggc ttgtccctcg gaaaggagag actgatgaaa accaacatgg
    17821 gggtggttga ccggctcgtc cggatcgcgg cggccgccgc gatcgtggtc ctctactgcc
    17881 tggaagtgat caacggagtt tgggccgtcg ttttgggaat cgtcgcggtc gttttcatcg
    17941 tgacgagctt cattggattt tgtcctctgt acgttccctt ccggatttca acgaaaaaaa
    18001 agccgaagcc gtccccctga gttcccgtct cgcgggccgg gagcggcggg atctccgttc
    18061 cctgtcccgc gttataaaga ctttgcccaa aagggcatgg tcttgccatt taagaacctg
    18121 accggagccg aggcttgacg agatcgtttt gacctgaatt ccatgagctc cctccggctg
    18181 tgaagatcca ttctccgccc tcctcagata aagaggagag cgtaaaaaaa cgtccttttc
    18241 cttgtccagt cccggtaaga tttttaaatg gcaagatata tcacccttcg g
//
LOCUS       DAOOXH010000048        18197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_2960_length_18197_cov_8.069287, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000048 DAOOXH010000000
VERSION     DAOOXH010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 18197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18197
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2960_length_18197_cov_8.069287"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PKV64_06930"
     CDS             complement(<1..189)
                     /locus_tag="PKV64_06930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HPN16851.1"
                     /translation="MKDDSAGSGVQNRRDFLKAAAAGAAGVSAVLAGFKPLLGDQRRV
                     PGEGRTAFAAAPLPRVRIG"
     gene            517..1551
                     /locus_tag="PKV64_06935"
     CDS             517..1551
                     /locus_tag="PKV64_06935"
                     /inference="COORDINATES: protein motif:HMM:NF027219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HPN16852.1"
                     /translation="MSASNVKSLVQSWETVRFELLNRRISDLNLKIEGSPAERFVQRL
                     RRELETKKFIFKPSFYLTDAWGCPDRTPVIGVPFYLADERLTRLEEEQNGEMEDPKTI
                     MMLFRHEAGHAINYAFKLWERSSWKEIFGSFTKPYRDIFRPDRFSRQFVRHIDAHPYG
                     RTYAQKHPDEDFAETFAVWLAPRSDWRNRYKHWPVLRKLKYVDRLMREIRRQKPRNIR
                     RKVVLPVEKMNLTLADFYGKKADVFRRSARGYVDDRLRQVFPPSRAEVLLPAVDLFRR
                     RRAELLDSIVRWSSLDEKEADAILRKLESRTKTLDLRYPQGKKRSREMEIAALAVALA
                     MDYAYTGRLA"
     gene            complement(2027..3097)
                     /locus_tag="PKV64_06940"
     CDS             complement(2027..3097)
                     /locus_tag="PKV64_06940"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16853.1"
                     /translation="MRKLRVALVYNAYADAQESAEDDQSGAAYLREMIFTIARAVRRL
                     GHKVTVIPLTDDLNYLQRRLRRLKPHVVFNQYDDVVHGALYEMRVAAFIRMLGFPITG
                     SPAIALGLSRHKYMAASLLQGAGVPIPGQTMLIERIGELNGRDWKFPLIVHASQEHAG
                     IGLDRHSVVHTKAALKDKVREILKTYRQPALVQDFLAGREFNVGVIGGRKLRVLPLAE
                     VDYSALPGTIPPIMSYAAKWIETTVEFQKTRVLCPAKTKPELTQAIGETAKKAFRVVG
                     GWGYGRVDIRLDSEGRPRVLEVNCNPCLDNDMGLARAAKRAGINYSALMGIIIKAAFE
                     GPPFDMNLPIFGLNNRPAPRPK"
     gene            complement(3339..3944)
                     /locus_tag="PKV64_06945"
     CDS             complement(3339..3944)
                     /locus_tag="PKV64_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HPN16854.1"
                     /translation="MIGRFRTTRLPPPTRHSLTCGGETVVFLWVRGRGRRLRLEVRED
                     GEIRVHSPARAGADAVLSFVAGKAAWILRTRAKALARRRLTWPEDRREQARLRTECER
                     RLQERLEACLPLAAAHNLPRPAVSFRMMKRRWGSCARDGRIVLNVRLAQLPPACIDYV
                     ILHELCHLRHHHHGRPFYDLLARLVPDGKERRTELARFRIA"
     gene            4011..5063
                     /locus_tag="PKV64_06950"
     CDS             4011..5063
                     /locus_tag="PKV64_06950"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPN16855.1"
                     /translation="MPNPKAAARSPRRPKVGLVLGSGGARGLVHFEIIEGLLRMDIPI
                     DLIAGSSIGAVVAGIYAAGGLPRLREDLQTMKREDFVRLFDPALSRYGFFAGRKVMVF
                     LRRYIPPETRLEDLGLPLGIVATDYETGRPVVFRKGNLLEAIRASISIPGVFTPVRVG
                     SKLLLDGGVVSPLPIDIAEQMGGHLIVAVSLLPTIGKLRGILPLGWKKRRGGDKPPPG
                     RLPLKEKIARRLPPGRKEERRWLRIADQWLTAKNGEAGEPEKTPNLIDIVTRTIDIMA
                     YTETRQMLAAHPPAVLFEFDFPDLGVLDFTRSQELLDAARRAFELKIPELTEKVKKRL
                     SLPMPPSFGKARREIR"
     gene            complement(5070..6983)
                     /locus_tag="PKV64_06955"
     CDS             complement(5070..6983)
                     /locus_tag="PKV64_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPN16856.1"
                     /translation="MTEPRTLPRLLETSADRFPGNPLLWEKKTDVYEPMTYARTRERV
                     HAFGAGLMSLGLNKGDRVALISEGRNDWVVSEMGILYCGAINVPISIKLDEPNDLKFR
                     LSHSGCRFVIVSRGQVEKIRRIKNDLPQLEKVIVLDELKSFESDEIYAGDILTAGESL
                     PPAAREAFAERWRSVQESDYANICYTSGTTADPKGIILTHRNYTANCEQSLDLVRCEE
                     DWLSLIILPWDHAFAHTCGVYTLMMRGASLASIQAGQTGMETLRNIPVNIKETRPHIL
                     LSVPSLAKSFRKNIEKGVREKGPKVEALFRKALKTAVAYNAEGCNRGRGRQALKKPLV
                     LLYDKILFSKIRENFGGRLQFFVGGGALLDIELQRFFYALGIPMFQGYGLTEAAPVIS
                     ANSGAAHKLGSSGRVAKGIEIRICDSSGTPLPVGGRGEIVIRGENVMAGYWKNEKATA
                     EALKNGWLYTGDLGYLDEDGYLYVLGRTKSLLIANDGEKYSPESIEEAIIESSPYIEQ
                     FMLYNNQSPYTVGLLVPNKEALLAWLKKSGLSARAPEGQEAVLRLLDGEIAKYREGGP
                     FAGQFPARWLPATVAVLGEGFTEQNHLMNSTMKIVRGKIEDFYRSRIDNLFTAEGKTI
                     DNPQNRMIISRFE"
     gene            7132..7485
                     /locus_tag="PKV64_06960"
     CDS             7132..7485
                     /locus_tag="PKV64_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16857.1"
                     /translation="MNADPQADPAARAKLDEILHPSTTADSFRVTVVFGHKLDSRYQK
                     AVFLAKRNPTYREEGEGEWIRHSAVYTPAEVEDLFDLFNIVHDWEHTEILVNHKPLPY
                     GHQLWLPLMWFYRIT"
     gene            complement(7797..10400)
                     /locus_tag="PKV64_06965"
     CDS             complement(7797..10400)
                     /locus_tag="PKV64_06965"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HPN16858.1"
                     /translation="MMFQFSPWAAVFALGVIVSIFLAVSAWSKRRSPGGRTFFWLMLA
                     LVWWQVFAILEFILVSSSDKILCAQFRYIAVSGVSLLYMLFCYDYHEFHARIPVVFRW
                     ILWIPGLVVAALAFTNPLHHLVWTSVTPLPGAKLSILIYNYGPAVWVHVGYACTLVLI
                     GTAILFGLIVKSKERPVRHQAVWLILGALAPGVANLVHLIDLGPKGLDLTPFGFVLSG
                     LFISRSFFRHHLLKITPVAFGSIFDYMTDAVIVFDDKNRVIEANPAAERLFEMPPEYV
                     GWPFDQILERWPEYRDRLASFLRMTGVRVFKGSRGANWLETRIFNIYSRSGEVKSWYL
                     IARDITESHTAEEERAAAAERINAQRKALLDLSFSRAAAEGDFPIAIREITEALARNL
                     EVERASFWLGNAREGLIRCIDLYERSKDHHAQGPVLMADRFPRYFEALDRDRVIDASA
                     AASDPRTREFTDVYFKPLGIGSLIDAPVRVSADVIGVISGEYVGPPRRWLEDEVRFVA
                     EIADAAAHAYISWEKRTIEDARRESEERFRKLVEGAPDAIVVQAEGVFAYLNEAAVRL
                     FGARSADQLLGRPFAERVRPEARPKLLERIKLINEKKRSFPHQEEIFLQLDGTPVETE
                     TASVPIRYRELDGALVFIRDIAERKRAEESLRSAFEEKVILLREVQSRVRNNMQIVTS
                     LLNHQAAAISDPALRQAFRASRDRIKAMSLVHEKLYRSEDLARVDFADYIQSLVVHLF
                     QVYQIPSERIRRTYDLSPSVFGVDVSIPLGLILNELLVNALRHAFPDDRTGEIFIRLE
                     EEQNGRHTLQVRDDGVGMPGPVDLAEAETIGFQLIGMLVEQIRGEVRIETGPGVSFTI
                     TFPDRPVEPPA"
     gene            complement(10506..11879)
                     /locus_tag="PKV64_06970"
     CDS             complement(10506..11879)
                     /locus_tag="PKV64_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HPN16859.1"
                     /translation="MPPPVEPFRIKMVEPLGRTTRRERDKLIRAAGFNVFNLPAEKVF
                     IDLLTDSGTSAMSDRQWAGIMMGDESYAGARSFFRFEKAVRDIFGFAHVIPTHQGRAA
                     ERILFESVLGRNSAVPSNIHFDTTRANIEVRNAKAVDLAGAAAYDPDADLPFKGDMDT
                     AKLESFLDRSAGRVPIVMMTVTNNSGGGQPVSLANIRKVSAVCRLRKIPFFFDACRFA
                     ENAWFIKRREKSYGSWTVRDIARKMFSYADGATMSAKKDALVNIGGFVTLNDSDLAAR
                     IKNMLIIGEGFPTYGGLAGRDLEAMAQGLQEVLDESYLEHRMRQVAYLGNLLDEAGVP
                     YFKPAGGHAVYVLADRLLPHIPRDRYPGWALTVALYRESGIRAVEIGGVMFGRKNPRT
                     GREEFPDLELVRLAIPRRVYTASHMVFVAQALGAIARKPERVKGVRIVEEPRFLRHFT
                     ARFEEID"
     gene            complement(11924..12826)
                     /locus_tag="PKV64_06975"
     CDS             complement(11924..12826)
                     /locus_tag="PKV64_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPN16860.1"
                     /translation="MSLVIVGSVAFDTIQTPEARRERIIGGSCTYSALAASYFTRPRI
                     VAVVGEDFPKPTADFLKKRGVDLKGLTVVPRGKTFFWEGRYGDDPNQRDTVRLDLNVF
                     RDFRPTLPSSYRDPDILFLANIGPGLQEHVLSQVKKPRIVAMDTIRHYIESEPEALER
                     VLQRTDIFFANDEEARLMTGELNLIRAGRRLVERGPRLVVLKKGEHGAFVFGRHWLFG
                     IMAHPCDRVVDPTGAGDSFAGAFLGFLDRAGTLRSPAVRKAAVYGSVLASFTIEDFGI
                     ERFKTMKPADVEGRFAALKKLVSV"
     gene            complement(12823..13770)
                     /gene="mtnP"
                     /locus_tag="PKV64_06980"
     CDS             complement(12823..13770)
                     /gene="mtnP"
                     /locus_tag="PKV64_06980"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HPN16861.1"
                     /translation="MTPRKAVGKPVKATAKKSQAIRKKAAPRAEIGILGGTGLYEIEG
                     IHNLEEVRLKTPWGDPSDEYILGALEGRKVAFLSRHGRGHRFSPSEINYRANVYGFKM
                     LGVERLISVNSVGSLREEIRPRDIVFADQFIDKTKRDCTFFGGGCVAHISLSEPVCPQ
                     LSRHCHVVAVGLGIRSHLGATYVCMEGPAFSTRAESKMHQVWGGDLIGMTAATEAKLF
                     REAEICYATMNLATDYDTWRPHEEGVTVGMIFENLRVNIVKAKEIIRKAVAALPPHDE
                     TACSCRHVLAAAVVTAAKHVPAATRKKLALLVPEYRTGR"
     gene            13927..15111
                     /gene="larC"
                     /locus_tag="PKV64_06985"
     CDS             13927..15111
                     /gene="larC"
                     /locus_tag="PKV64_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HPN16862.1"
                     /translation="MKFVYFDASAGLSGDMISAALLDLGVPPAAYRAAVDGLRLPVRV
                     KVGIVERAHLRARKIDVRILGKGSPARRWKDVSSLVKKADLPAAVKERGLSVFEALFK
                     AEAKVHGEAIDQVHLHEAGADDALVDILGACWLAESLGVGEFYCSPLNVGGGSVRTAH
                     GVLPVPAPAAAELLRGIPVYSAHVQTELVTPTGAALVKALVSKFLPFPEITYDRIGCG
                     AGGRDLETLPNILRAFSGGRQSFSPDKRVYLLEAAVDDASPQVLAAFMDRALEGGALD
                     VYFTPVVMKKNRLGTKLTLLAEARRLEDLIAEVFTETTSIGLRYFPVERRALVRRIET
                     VRLDGRNVRVKIALLDGRPVNVQPEYEDCLALARAKKLPLKDVLRRAAEAAGKPRRMK
                     SP"
     gene            15135..15716
                     /gene="folE"
                     /locus_tag="PKV64_06990"
     CDS             15135..15716
                     /gene="folE"
                     /locus_tag="PKV64_06990"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPN16863.1"
                     /translation="MDLAKIERGVRLILEGAGENPDRPDLRGTPRRVARMYAEILGGL
                     HESPETHLRQVHEERHDEMVLIKNIPLYSMCEHHMLPFAGVAHVAYIPKGGRIVGLSK
                     IARVVETLSRRLQVQERLTKQVADIIMDHLDPLGVIVVIEAEHMCVSMRGAKKPKSVT
                     VTSAVRGLFRSRSVTRLEAISLIRGGFSSGKEV"
     gene            15700..17676
                     /gene="metG"
                     /locus_tag="PKV64_06995"
     CDS             15700..17676
                     /gene="metG"
                     /locus_tag="PKV64_06995"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPN16864.1"
                     /translation="MEKKSKPPFYVTTPIYYVNDVPHIGHAYTTIIADALARFMKMDG
                     REVFFLTGTDEHGQKVEKAAAEHGVDPRTWADRVVVRFFDLWKALDCEPDFFIRTTME
                     LHEKGVQKMFRLLLDKGDIYKGEYKGWYCVSDENFLTDDVPASADGTKTCPDCGRKAQ
                     VVSEETYFFRLSAFQQRLLDHYRDHPRFVRPQSRMNEVASFVRGGLKDLSITRTTVKW
                     GVPVPGDPAHTIYVWYDALHNYVTGIGYDRDPALFEKFWPATVHLIGKDILRFHAVFW
                     PAFLLAAGLPLPECVFGHGWWLKDEAKMSKSKGNVLDPYVLLRAMGPDPLRYFLLREV
                     PIGLDGNFSHEGFIHRVNSDLANDLGNLVSRALTMVRNYFQGTIPPPAPEEPDDAEIR
                     KTFGEMKDRAVAHYESYALNKALEEIWSFITAVNKYLADKAPWKLAKDPALRPQLARV
                     LHQAVAAIRGTAALIAPVMPGSAQKMWEFTGEPGRVRDVRFDTLRFEDLPAGRPMKEP
                     EQLFPRVNLKEFLAEDAGTAKEKTMNEIPSPPERPAAPAVSEAPLITYDEFAKLDLRV
                     AHVLEAVKVEGAKKLLKLKIDLGTEQRQIVAGVAESYAPEQLVGKKFIIIANLQPAVI
                     RGVESQGMLLAAAAGGKAMIPFFEPDVPAGTKVK"
     gene            complement(17683..>18197)
                     /locus_tag="PKV64_07000"
     CDS             complement(17683..>18197)
                     /locus_tag="PKV64_07000"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPN16865.1"
                     /translation="GANAFSAEAVKAVYALKHRDRKKPLPIVAADAGAVLSLGRRLPR
                     VFFDLIRQFWPGPLNLVVHARPVFPPEMLGPDETIAVRVPGLSWLREFLADLGVPLTS
                     TSANLSGNSENADPLMLTRDFKNRDVTIIDAGRTPGGLASTIVDVTMSPPKIIREGAI
                     PVECLAPFLA"
BASE COUNT         3410 a         5780 c         5793 g         3214 t
ORIGIN      
        1 gccgatgcgg acgcggggaa gcggagcggc ggcgaaagcg gtccggcctt cgccaggaac
       61 ccggcgctga tcgccgagaa gaggcttgaa accggccaag acagcgctca cgccggcggc
      121 cccggcggcg gcggctttca gaaaatcacg gcggttttga acgccggagc cggcggaatc
      181 gtctttcatg tcatcctcct tgccggtatc cgccccgcgg acgggcggga atgattatat
      241 ccaaatcgga gacacattgc ttattcccca aatgatcacg gtttacggcc ggtcaaaaca
      301 caaaatcgaa aaagcgagga atgaagctaa aatgaggcga atatgcatgg ccccacctgt
      361 ctaattgttt catattaata taaatacata ttattggatt atgccttcaa aaatccgggt
      421 cgattcttct tgacaagcct ctcttttctt cctatgttgt cgacttaagg gaggcccggc
      481 ggtctcccgt cccgtcaggg aggaaatccc ccgatcatga gcgcctcgaa tgtcaagagt
      541 ctcgtccaaa gctgggagac cgtgcgcttt gagctgctga accgcaggat ttccgacctc
      601 aacctgaaga tcgagggatc cccggccgag cgtttcgtcc aacggctgcg ccgcgagctc
      661 gagaccaaga aattcatctt caaaccgtcc ttctacctga ccgacgcctg gggctgcccc
      721 gaccggacac cggtcatcgg cgtccccttc tacttggccg atgagcggct gacccgcctc
      781 gaggaggagc aaaacggcga aatggaggat cccaagacga tcatgatgct cttccgccac
      841 gaggccgggc atgcgatcaa ctacgcgttc aagctttggg agcggtcgtc ctggaaggag
      901 atcttcggtt ctttcaccaa accctatcgg gacatcttcc ggccggaccg cttcagccgg
      961 caattcgtcc gccatatcga cgcccatccc tacggccgga cctacgccca aaaacatccc
     1021 gacgaggatt tcgccgaaac cttcgccgtc tggctcgccc cgcggagcga ctggcggaac
     1081 cgttataaac actggcccgt tctgcgcaag ctcaagtacg tcgaccgctt gatgagggag
     1141 atccgcaggc agaagccgcg caacatccgc cgcaaggtcg tcctgccggt cgaaaagatg
     1201 aacctgaccc tggccgattt ctacggcaag aaggccgatg tcttccgccg atccgccagg
     1261 gggtatgtcg acgaccggct ccggcaggtc ttcccccctt cgcgggccga agtcctcctc
     1321 ccggccgtgg acctgttccg ccggcgccgg gcggagcttt tggacagcat cgtccgctgg
     1381 tcgagcctcg acgaaaaaga agccgacgcc atccttcgca agctcgaaag ccggacaaag
     1441 accctcgacc tccgctatcc tcagggtaag aaacgatcgc gggaaatgga gatcgccgcc
     1501 ctggccgtgg ccctggccat ggactacgcc tacaccggcc gcctcgcctg atctgatcgg
     1561 ccgcgattcc atccgggatc gtcggtttcc cctatccgcg acctcccggc cccgctccat
     1621 gtcgatccgc gggtaaaaat aggacggcga tcggcatgga aagcgcatcg cctgaggacg
     1681 gaacggatgg ccttttgttt ccgcgaacag cggagagagc gccggatgat cgccaaggag
     1741 gcatcactga gcggacatta aggcaaggcc ggggaacggg aaaagcaatc ctggcgatcg
     1801 ccaaggagcc gggcccgcat cgggcgttgg aaaagccgcc tcttccgccg cggaaccggg
     1861 gggaaggcgc cgttggaaaa gccgccgccg actcaagcgc cgcttttccc agccttaaca
     1921 aatcgggaaa tggataaaca ggtaccgata tgaatcggga aatggataaa caggtaccga
     1981 tatgaatcgg gaaatggttg accaggtacc gattggcggg ccggatttat ttgggacggg
     2041 gggccgggcg gttgttgagt ccgaaaatcg gcaggttcat atcgaacgga gggccctcga
     2101 aggccgcctt gatgatgata cccatgagtg cggaataatt gatgcccgcc ctcttggccg
     2161 cccgggcgag tcccatgtcg ttgtccaggc aggggttaca gttcacctcg agaacgcgcg
     2221 gccggccctc ggaatccagg cggatgtcga cccggccgta tccccacccc ccgaccaccc
     2281 ggaaggcttt cttggcggtt tcgccgatgg cctgggtcag ctccggcttg gtcttggccg
     2341 ggcagaggac ccgcgtcttc tggaattcca cggtggtctc gatccatttg gccgcatagg
     2401 acatgatcgg gggaatcgtc ccgggcaggg cggaataatc gacctcggcc aggggcaaaa
     2461 cgcgaagctt cctcccgccg atgaccccga cgttgaactc ccgcccggcg agaaaatcct
     2521 ggacgagcgc cggctggcgg tatgtcttca ggatctcgcg gaccttgtcc ttcaaggcgg
     2581 ccttggtatg gacgacggaa tgacgatcca ggccgatgcc cgcgtgctcc tggctggcgt
     2641 ggacgatgag ggggaatttc cagtcgcggc cgttcagctc gccgatccgc tcgatcagca
     2701 tggtctggcc cggaatgggg acgccggcgc cctgcagcag gctggccgcc atgtacttgt
     2761 ggcggctgag gccgagggcg atggccggcg acccggtgat cggaaatccc agcatccgga
     2821 tgaaggccgc gacccgcatc tcgtacaagg ccccgtgaac gacgtcgtcg tattggttga
     2881 agacgacgtg cggcttcagc cggcggaggc ggcgctggag atagttgaga tcatcagtca
     2941 acgggatgac ggtcaccttg tgcccgagcc ggcggaccgc ccgggcgatg gtgaagatca
     3001 tctcccgcag atacgccgcg ccgctctggt cgtcctcggc ggactcctgg gcgtcggcgt
     3061 aggcgttgta aacgagcgcg acccggagtt tcctcatctc ttcctcaatt tcaatggaac
     3121 ggcgctttca tcataacgaa ggacgcgcgc cggcgcaagc gcgcgcatgg aatcaccggt
     3181 caaaatcccg atccttcctc ggcaacagtt tatatgattc aacggccctg cgttcggtca
     3241 catggctcta cgagttgaca cggcggcgcg gccgggggga cgggcgggcc gggatgtcct
     3301 ccgcggcggg aatatcccgg gaggagcggc cggccgcctc aagcgatccg gaagcgggcg
     3361 agctccgttc tccgctcttt cccgtcgggg acgaggcggg ccagcaaatc gtaaaacggg
     3421 cgcccgtgat gatgatggcg gagatggcag agctcgtgga gaatcacata gtcgatgcaa
     3481 gccggcggaa gctgggccaa ccggacattg agcacgatcc gcccgtcccg ggcgcagctc
     3541 ccccagcgcc gcttcatcat ccggaaggaa accgccggcc gggggaggtt gtgggccgcg
     3601 gcgaggggga gacaggcctc caagcgttcc tgaagccggc gttcacattc ggtccggagc
     3661 cgggcttgtt cccggcgatc ctccggccac gtcagccgcc ggcgggccag ggccttggcc
     3721 cgcgtccgga gaatccaggc cgcctttccg gccacgaacg acaggaccgc gtccgccccg
     3781 gcccgggcgg gggaatggac gcggatctct ccgtcctccc ggacctcaag gcgcagacgg
     3841 cggccccggc cccgaaccca aaggaaaacg acggtctccc cgccgcaggt caggctgtgg
     3901 cgcgtcggcg gcggaagacg cgtcgtccgg aatcgtccga tcatcccggc catcttaccc
     3961 gagaacgggc gcccgttgcc attcgccggc gatcatgata tttttttttc atgccgaatc
     4021 cgaaagccgc cgcccgctcc ccgcgccgcc ccaaggtcgg cctcgtcctc ggaagcggcg
     4081 gagcccgggg cctcgtccac ttcgaaatca tcgaagggct tctccggatg gacatcccga
     4141 tcgacctcat cgccggttcg agcatcggcg ccgtcgtcgc cggcatctac gccgccggcg
     4201 ggcttccccg ccttcgggag gaccttcaaa cgatgaagcg ggaggatttc gtgcggcttt
     4261 tcgatccggc tctttcccgc tatggattct tcgccggccg gaaagtgatg gtcttcctcc
     4321 gccggtatat cccgcccgaa acgagactcg aggacctcgg ccttcccctg ggcatcgtgg
     4381 ccaccgatta cgagaccggc cggccggtcg ttttccgcaa aggcaatctc ctggaggcga
     4441 tccgggcgag catttccatc cccggcgtct tcaccccggt ccgcgtcgga tcgaagcttc
     4501 tcctggacgg cggagtcgtc tccccgctgc cgatcgacat cgccgaacag atgggcggcc
     4561 acctgatcgt cgccgtcagc cttctgccga ccatcggaaa gctccgggga atcctccccc
     4621 tgggctggaa aaaacggagg ggcggggaca agcccccccc cgggcgcctg ccgctgaagg
     4681 agaaaatcgc ccgccgcctc ccgcccggcc ggaaggaaga gcgccgctgg ctgaggatcg
     4741 ccgaccagtg gctgacggcg aagaacgggg aagccggcga gccggagaag acgccgaatc
     4801 tgatcgacat cgtcacccga acgatcgaca tcatggctta cacggaaacc cggcagatgc
     4861 tggccgccca tccgccggcg gttctcttcg agttcgactt tcccgacctc ggcgttctcg
     4921 atttcacccg gagccaggag cttctggacg ccgcccgccg ggcgtttgag ctcaaaattc
     4981 ccgaattgac cgaaaaggtt aaaaaacgcc tgtccctgcc gatgcctccg tcgtttggga
     5041 aagcccggcg cgagatccgc tgagcccggt tactcgaacc gggagatgat catccggttc
     5101 tggggattgt cgatggtctt tccctcggcc gtgaacaggt tatcgatccg ggaccggtaa
     5161 aagtcctcga tctttccccg gacgatcttc atggtcgagt tcatgagatg gttctgctcg
     5221 gtgaatccct cgcccaggac ggcgacggtc gccggcagcc agcgggccgg gaactggccg
     5281 gcgaaaggac cgccctcgcg atacttggcg atttccccgt ccaagagccg gagaacggcc
     5341 tcctgcccct cgggcgcgcg ggccgacaaa ccgctctttt tcagccaggc gaggagggct
     5401 tccttgttgg gaacgagaag cccgaccgta tagggcgact ggttgttgta aagcatgaac
     5461 tgctcgatgt aaggcgagga ttcgatgatc gcctcctcga tggattcggg actgtatttc
     5521 tccccgtcgt tggcgatcag caggctcttg gtccggccga ggacatagag atagccgtcc
     5581 tcatcgagat agccgagatc gccggtgtag agccagccgt tcttgagcgc ctccgccgtg
     5641 gccttttcgt tcttccagta gccggccatg acgttttcgc cgcggatgac gatttctccc
     5701 cggccgccga ccggcagggg cgtgccggag gagtcgcaga tgcggatttc gatccccttg
     5761 gccacccggc cggaggagcc cagcttatgg gccgcgccgc tgttggccga gatgaccggg
     5821 gcggcctcgg tgagaccgta gccctggaac atcgggatgc caagggcgta gaaaaaccgc
     5881 tggagctcga tgtcgaggag agctccgccg ccgacgaaaa actggagccg gccgccgaaa
     5941 ttctcccgga tcttgctgaa gaggatcttg tcgtaaagga gaacgagcgg cttcttcagg
     6001 gcctgccggc cgcggccccg gttgcagccc tcggcgttgt aggcgacggc cgttttgagg
     6061 gccttccgga acagggcctc gactttcggg cctttctccc gcacgccctt ctcgatgttc
     6121 ttccggaagc tcttggccag ggaagggacg ctgaggagaa tgtggggccg ggtttccttg
     6181 atattgacgg ggatgttgcg cagcgtttcc atgcccgtct ggccggcctg gatcgaggcc
     6241 aggctcgccc cgcgcatcat cagcgtataa acgccgcagg tgtgggcgaa ggcgtgatcc
     6301 cagggaagga tgatcaggct gagccagtcc tcctcgcacc ggaccaagtc gagcgactgc
     6361 tcgcagttgg ccgtgtagtt gcggtgggtc aggatgatgc ccttgggatc ggccgtcgtc
     6421 cccgacgtat agcagatgtt ggcgtaatcc gattcctgga cggaccgcca acgctcggcg
     6481 aaggcttccc gggccgcggg cggcagggac tccccggccg tgaggatgtc gccggcgtat
     6541 atctcgtcgc tttcaaacga cttgagctcg tccaggacga tgaccttttc cagctggggg
     6601 aggtcgttct tgatccttcg gattttttcc acctggccgc gcgagacgat cacgaagcgg
     6661 cagcctgaat ggcttaggcg gaacttgagg tcgttcggct cgtcaagctt gatcgagatg
     6721 gggacgttga ttgccccgca gtagagaatg cccatctcgc tcacgaccca atcgttccgg
     6781 ccttcggaga tcagggcgac tcggtcgccc ttattgaggc ccaaggacat caacccggcc
     6841 ccgaaggcgt gaacccgctc gcgcgtccgg gcgtaggtca tcggctcgta gacgtcggtc
     6901 ttcttctccc aaagcagcgg attgccggga aagcggtcgg cgctggtttc caggagccgg
     6961 ggcagggtcc ggggttcggt catggcatcc tcctccgtgg cctatgactg cgggcgcggg
     7021 ccctcgtcga tgatgggtgt attctatact aaaaccgcgc ccggaaggga atccccgaat
     7081 tccctttcac cgcggttgtc ccggccggcc gttttcgggt agaatgtttc catgaacgcg
     7141 gatccccaag ccgacccggc ggcccgagcc aaactggacg aaatcctcca tccgagcacg
     7201 accgccgatt ccttccgcgt taccgtcgtg ttcggccaca agctggattc caggtatcag
     7261 aaggccgtct tcctggccaa gcgcaatccg acctaccggg aggaagggga aggcgaatgg
     7321 atccggcatt cggccgtcta cacgccggcc gaggtcgagg atttgttcga cctgttcaac
     7381 atcgtccacg actgggaaca caccgaaatc ctggtcaacc acaagcccct cccctacggc
     7441 caccagctct ggctccccct gatgtggttt taccgcatca cctgagccgc gccccggacc
     7501 accggctgtt ttcatcctgt ccctgagcgg ggaaaagccc gtccgtccgt ttttggattc
     7561 cgctgggatt tcccggcggg aggagacggg cggctgaaag ctcatcgtcg ccggcggaga
     7621 ttcctctcga ggaaagaagg cggcggatct tccccttcat ggacttcgag ggggaacggc
     7681 cgaggaatcg tcgtccccgc cttccgatcc cgcccttgtc ccgccgagga tgacgcccgg
     7741 gattcccccc tgaacggatt tccgccctgg atccgaagga gaaaatcctc cgctcgtcag
     7801 gccggaggct cgacgggacg gtcgggaaac gtgatcgtga acgaaacccc cggcccggtc
     7861 tcgatcctga cttcgccccg gatctgctcg acaagcattc cgatgagctg gaagccgatc
     7921 gtctcggcct cggccaggtc gaccggcccg ggcatcccga ctccgtcgtc ccggacctgg
     7981 agcgtgtgcc ggccgttttg ttcctcctcc agccggatga agatctcccc cgtccggtcg
     8041 tcggggaagg catgccggag ggcgttgacg aggagctcgt tgaggataag acccaagggg
     8101 atggagacgt cgacgccgaa aaccgagggg gacaagtcgt acgtccgccg gatccgttca
     8161 gacgggatct ggtagacctg gaacaggtgg acgaccagac tttggatata atcggcgaaa
     8221 tcgacgcggg ccaggtcttc cgaccggtaa agcttttcat ggaccaggga catcgcctta
     8281 atccggtccc ggctggcccg gaacgcttga cgcagggcgg gatcggaaat cgcggcggcc
     8341 tggtggttga gcaggctcgt aacgatctgc atgttgttgc ggacccggct ttggacttcc
     8401 cggagaagga tgactttctc ctcaaaggcg gaccggaggc tttcctcggc ccgcttgcgc
     8461 tcggcgatgt cccggatgaa gacgagggct ccgtcgagct cccggtagcg gatgggaacg
     8521 gaggccgttt ccgtctccac cggcgtcccg tcgagctgga ggaagatttc ctcctggtgg
     8581 ggaaagctcc gtttcttctc gttgatcagc ttgatccgct ccaagagctt gggccgggcc
     8641 tcgggacgga cgcgctcggc aaagggccgg cccagcagct ggtcggcgga ccgggctccg
     8701 aagaggcgga cggcggcctc gttgagatac gcgaagactc cctcggcttg gacgacgatc
     8761 gcgtcgggcg cgccctcgac gagcttccgg aaccgctcct cgctctcccg gcgggcgtcc
     8821 tcgatcgtcc gcttttccca gctgatatag gcatgggcgg ccgcgtcggc gatttcggcg
     8881 acgaatcgga cctcgtcctc cagccagcgg cgcggcgggc cgacgtattc cccgctgatg
     8941 acgccgatga cgtcggccga gacccgcacg ggcgcgtcga tcagggagcc gatcccgaga
     9001 ggtttgaagt aaacgtcggt gaattcccgg gtccgcggat cagaggcggc ggcggacgcg
     9061 tcgatcaccc ggtcccggtc cagggcttcg aagtaccggg gaaaccgatc ggccatgaga
     9121 acgggcccct gggcatggtg atccttcgac cgctcataaa ggtcgatgca gcggatgagc
     9181 ccttcccggg cattgcccag ccagaagctc gcccgctcga cttcaagatt ccgggccagg
     9241 gcctccgtga tctcccggat ggcgatcggg aaatcgccct cggccgccgc ccgggagaag
     9301 gataaatcca aaagcgcttt tctctgggca ttgatgcgct cggccgcggc cgcccgttcc
     9361 tcctcggccg tatggctctc ggtgatgtcc cgggcgataa gataccagga tttgacctcg
     9421 ccggaacggc tgtagatatt gaaaatccgc gtttccagcc agttcgcgcc gcgcgacccc
     9481 ttgaagacac gaactccggt catgcgaaga aaggaggcga gacggtcccg gtactccggc
     9541 caacgctcca ggatttgatc gaacggccag ccgacatact cgggaggcat ctcgaaaagc
     9601 cgctcggccg cgggattggc ttcgatcacc cggttcttat cgtcgaagac gatgaccgcg
     9661 tcggtcatgt aatcgaagat gctgccgaag gcgaccgggg tgatcttcag gagatgatgc
     9721 cgaaaaaaac tccgggagat gaaaagcccg gaaaggacga aaccgaacgg cgtgaggtcc
     9781 aaacccttcg gtcctaggtc gatgagatgg accaggttgg ccaccccggg cgcgagcgcg
     9841 cccaggatga gccaaacggc ttggtgccgg accggccgtt ccttgctctt gacgatcaaa
     9901 ccgaaaagaa tggccgttcc gatcagcacc agagtgcagg catagcccac gtggacccag
     9961 acggccgggc cgtagttata aatcagaatc gagagcttcg ccccggggag cggggtgacc
    10021 gatgtccaga cgagatggtg gaggggattg gtgaaggcca aggccgcgac gacgagcccc
    10081 ggaatccaga gaatccaccg gaatactacg ggaatccggg catgaaactc gtgataatcg
    10141 taacagaaga gcatgtacag cagggagacg cccgacacgg cgatatatct gaactgggcg
    10201 cagaggattt tgtcggacga cgaaaccagg atgaattcca gaatggcgaa gacttgccac
    10261 cagacgaggg cgagcatcaa ccagaaaaac gtccggccgc cgggcgagcg gcgcttggac
    10321 caggctgaga ccgccaggaa tatggaaacg atgacgccga gggcgaagac cgccgcccag
    10381 ggggaaaatt gaaacatcat cgccatgctt ctccaaaggg atgcggaata tcgcatcccc
    10441 gcgccccgtg attataccaa agacggggga tcgccgtcag gaaagccggg acggagcgcg
    10501 gacgctcagt cgatctcttc gaagcgggcg gtgaaatgcc ggaggaagcg gggttcctcg
    10561 acgatccgaa cgcccttgac ccgctccggc ttccgggcga tcgcacccag agcctgggcc
    10621 acgaagacca tgtgcgaagc cgtgtagacg cgccggggga tcgccagccg gaccagttcc
    10681 agatccggga actcctcccg gccggtccgg ggattcttcc gcccgaacat cactccgccg
    10741 atttcgacgg cccggatccc cgattcacgg tagagggcca cggtcagagc ccagccggga
    10801 tagcggtccc gggggatatg cggaagaagc cggtcggcca ggacgtagac ggcgtggcct
    10861 ccggccggct tgaaatacgg cacgccggcc tcgtcaagca ggttccccaa ataggccacc
    10921 tgcctcatcc gatgctccaa gtaagattcg tccaggactt cctgcagtcc ctgggccatc
    10981 gcctcgaggt cccgtccggc caagccgccg tacgtcggga atccctcccc gataataagc
    11041 atgttcttga tccgggcggc caagtccgag tcgttgaggg tgacgaatcc gccgatattg
    11101 accagcgcgt ccttcttggc gctcatcgtc gccccatcgg cgtaggaaaa catcttccgg
    11161 gcgatgtccc ggaccgtcca gcttccgtag ctcttctccc ggcgcttgat gaaccaggcg
    11221 ttttcggcga accggcaggc gtcgaagaag aacgggattt tccggagacg gcagacggcc
    11281 gagaccttcc ggatgttggc cagcgaaacc ggctggccgc ccccgctgtt gttggtcacg
    11341 gtcatcatca cgatgggcac gcggccggcg ctgcggtcca agaacgactc gagcttggcc
    11401 gtatccatgt cgcccttgaa cggcaggtcg gcgtccggat cataagcggc ggctccggcc
    11461 aggtcgacgg ccttggcgtt ccggacctcg atgttggccc gggtcgtgtc gaaatgaatg
    11521 ttgctcggca cggcgctgtt ccggccgaga accgattcga agagaatccg ctcggccgcc
    11581 cggccctggt gggtggggat gacgtgggcg aagccgaaga tgtcgcggac cgccttctca
    11641 aaccggaaga agctccgggc tccggcgtag gactcatcgc ccatcatgat tccggcccat
    11701 tgtcggtcgc tcatggccga ggttccagaa tcggttaaaa gatcgatgaa cactttttcg
    11761 gcgggcaggt tgaagacgtt gaacccggcg gcccggatca gcttgtcgcg ctcgcgcctt
    11821 gtggtccggc ccagcggctc gaccatcttg atccggaacg gctcgacggg cgggggaatc
    11881 atgctcataa acgacctccc atgattatgg aatgaatgcg gtttcatacg gagacgagtt
    11941 ttttcaaggc ggcgaagcgg ccttcgacgt cggccggttt catggtcttg aaccgctcga
    12001 tgccgaaatc ctcgatcgta aacgaggcca ggacgctgcc gtatacggcg gctttgcgga
    12061 cagccgggga ccggagggtc ccggcccggt cgagaaagcc gagaaacgcc ccggcgaagc
    12121 tgtctccggc gccggtcgga tcgacgaccc ggtcgcacgg atgggccatg ataccgaaaa
    12181 gccaatgccg gccgaacaca aaagccccat gctccccttt cttcaggacg acgaggcggg
    12241 ggccgcgttc gaccaggcgg cggccggccc ggatgaggtt cagctctccg gtcatcagcc
    12301 gggcctcctc gtcattggcg aaaaagatat ccgtccgctg gaggacgcgc tcgagggcct
    12361 cgggctccga ctcgatgtaa tggcggatcg tgtccatggc cacgattcgg ggcttcttca
    12421 cctgcgacag gacgtgttcc tgcaaaccgg ggccgatgtt ggccaggaaa aggatgtcgg
    12481 ggtcgcggta agacgagggc agggtcggac ggaagtcccg gaagacgttc agatcgagcc
    12541 ggacggtgtc ccgctggttg ggatcgtcgc cgtagcggcc ttcccagaaa aacgtcttcc
    12601 ccctgggaac aaccgtcaat cccttgagat cgacgcctcg ttttttcaga aaatcggccg
    12661 tcggcttcgg gaaatcctcg ccgacgacgg ccacgatccg gggccgggtg aaatagctgg
    12721 cggccagcgc gctgtaggtg caggagccgc cgatgatgcg ctcccggcgc gcttccggcg
    12781 tttggatcgt gtcgaaggcg accgatccga cgatgaccag gctcatcggc cggtccggta
    12841 ttcaggaacg agcagggcca gctttttccg ggtggccgcc gggacgtgct tggccgcggt
    12901 gacgacggcc gccgccagga catgccggca ggaacaggcc gtctcgtcgt ggggcggaag
    12961 cgcggccacg gctttccgga tgatttcctt ggccttgacg atgttcaccc gcaggttttc
    13021 gaaaatcatc ccgaccgtga ctccttcctc gtgcggccgc cacgtgtcgt agtcggtggc
    13081 caggttcatc gtcgcgtagc agatctcggc ctcgcggaag agcttggcct cggtggccgc
    13141 ggtcatcccg atcaaatcgc ccccccagac ctgatgcatc ttcgattccg cccgggtcga
    13201 aaacgccggc ccctccatgc agacgtaagt cgcccccagg tgggagcgga tgccgagacc
    13261 gacggcgacg acgtggcaat gccgggacaa ctgggggcaa accggctcgg acagagagat
    13321 atgggcgacg cagcccccgc cgaaaaaggt gcaatcgcgc ttcgtcttgt cgatgaactg
    13381 gtcggcgaag acaatgtcgc ggggacggat ctcctcgcgg agcgagccga cggagttgac
    13441 cgagatcaac cgctcgacgc cgagcatctt gaagccgtac acgttggccc ggtagttgat
    13501 ctcggacggg ctgaaccggt gaccccggcc gtgacggctg aggaaagcca ccttgcggcc
    13561 ttccagggcg ccgagaatat actcatccga gggatccccc cacggggttt tcagccggac
    13621 ttcctccagg ttgtggatgc cctcgatctc gtataatcct gtcccgccga ggatgccgat
    13681 ttcggcccgg ggagctgctt ttttccggat cgcctgagat tttttcgcgg ttgctttcac
    13741 cggcttgccg acggcttttc tcggagtcat gggatctcct tcttaaggaa aggataaaga
    13801 tatcttttcc cccgcgggga gtcaacttga ggggggaatc cgccctatcc acggccgccc
    13861 atttgtgcta tcgttattca tcgcgcggcc tgctcggcgg acgggcggct ccgcaggaaa
    13921 accaccatga aattcgtcta tttcgacgcc tccgccgggt tgagcggcga tatgatctcg
    13981 gccgccctgc tcgacctggg cgtcccgccg gccgcctatc gggccgccgt cgacggcctc
    14041 cgccttcccg tccgggtgaa ggttgggata gtcgaacgag cccatctaag agcccggaaa
    14101 atcgacgtcc ggatcctggg aaaaggctcg ccggcccgcc ggtggaagga tgtctccagc
    14161 ctggtcaaaa aggcggatct cccggccgcg gtcaaggaga ggggcctgtc cgtgttcgag
    14221 gccctgttca aagccgaggc gaaagtccac ggcgaagcga tcgatcaagt tcacctccac
    14281 gaggccgggg cggacgacgc cctggtcgac atcctggggg cctgctggct ggccgaatcg
    14341 ctcggcgtcg gcgaattcta ttgctctccc ctgaatgtcg ggggaggatc ggtccggacc
    14401 gcccacggcg tcctcccggt tccggccccg gccgcggccg agcttctccg ggggatcccg
    14461 gtctattccg cccacgtcca aaccgagctc gtcactccga cgggagccgc gctcgttaaa
    14521 gcgctcgtct cgaaatttct ccccttcccg gagatcacat acgaccgaat cggctgcggg
    14581 gccggcgggc gggacctgga aacccttccc aacatcctcc gcgccttctc cggcgggcgg
    14641 caaagcttct ccccggacaa gcgggtctat cttttggagg ccgccgtcga cgacgccagc
    14701 ccccaggtcc tggccgcctt catggaccgg gccctggaag ggggggccct cgatgtttac
    14761 ttcacgcccg tcgtcatgaa aaagaaccgg ctggggacga agctgaccct gctcgccgag
    14821 gcccggcggc tggaggacct catcgccgag gtcttcaccg aaaccacctc gatcggtttg
    14881 cggtatttcc ccgtggagcg gcgggcgctt gtccgccgga ttgagaccgt ccggctggac
    14941 ggccggaacg tgcgggtcaa aatcgccctt ctcgacggcc ggccggtcaa cgtccagccg
    15001 gaatacgaag actgcctcgc cctggcccgg gcgaaaaagc tgcccctcaa agatgtcctg
    15061 cggagggcgg ccgaggccgc gggcaagcct cgaaggatga agagcccatg acaccgaagc
    15121 ccaaggccaa ggggatggat ctcgcgaaaa tcgagcgcgg cgtccgactg attctcgagg
    15181 gggccggcga gaacccggac cgcccggacc tgcgcggcac gccccggcgg gtcgcccgga
    15241 tgtacgccga aatcctcggc ggccttcatg aaagccccga gacccacctc cgccaagttc
    15301 acgaggaacg ccacgacgag atggtcctga tcaagaacat ccccttgtac tcgatgtgcg
    15361 agcaccacat gctgccgttc gccggcgtcg cccacgtcgc ctacattccc aagggcggcc
    15421 ggatcgtcgg cctctccaag atcgcccggg tggtggaaac gctgtcccgc cggctccaag
    15481 tccaagagcg gctgaccaag caggtcgccg acatcatcat ggatcacctg gacccgctcg
    15541 gcgtcatcgt cgtgatcgag gccgagcaca tgtgcgtctc catgcggggg gccaagaagc
    15601 ccaagtccgt caccgtcacc tcggccgtcc gcgggctgtt ccgaagccgt tcggtgaccc
    15661 ggcttgaagc catttccctg atccggggag gattttccag tggaaaagaa gtctaagcct
    15721 ccattttacg tcacgacgcc gatctattat gtcaacgacg tcccccatat cggccacgcc
    15781 tatacgacga tcatcgccga cgccttggcc cggttcatga agatggacgg acgggaggtg
    15841 ttctttctca ccgggacgga cgagcacggc cagaaggtgg aaaaggccgc ggccgagcac
    15901 ggcgtcgatc cgcggacctg ggcggacagg gtcgtcgtcc ggttcttcga tctttggaag
    15961 gccctcgact gcgagcccga tttcttcatc cggacgacca tggagctcca cgagaagggc
    16021 gtccagaaga tgttccgcct cctcctcgac aagggcgaca tctacaaggg cgagtacaag
    16081 ggctggtact gcgtctcgga cgagaatttc ctcaccgacg acgttccggc ctcggccgac
    16141 ggcacgaaaa cgtgtccgga ctgcggccgg aaagcccagg ttgtttccga ggagacgtat
    16201 ttcttccgcc tctccgcctt ccagcagagg ctgctcgatc attaccggga ccatccccgg
    16261 ttcgtccggc cgcaaagccg gatgaacgag gtcgcgagct tcgtccgcgg cggcctcaag
    16321 gacttgagca tcacccggac gacggtcaag tggggcgttc ccgttccggg ggacccggcc
    16381 cacacgattt atgtctggta tgacgccctg cacaactacg tgacggggat cggctacgac
    16441 cgggacccgg ccctgttcga gaaattctgg ccggccaccg tccacctcat cggcaaggat
    16501 atccttcgct tccacgccgt tttctggccg gcttttctcc tggccgccgg cctgccgctt
    16561 cccgaatgcg tcttcggcca cggatggtgg ctgaaggacg aagccaagat gtccaaatcg
    16621 aagggcaacg ttctggatcc ctatgttctt ctccgggcga tgggtcccga ccctctgcgg
    16681 tatttcctcc tgcgggaggt tccgatcggc ctggacggga atttcagcca cgagggtttc
    16741 atccaccggg tcaactccga cctggccaac gacctgggca acctcgtcag ccgggccctg
    16801 accatggtcc gcaattactt ccaggggacg atcccccccc ccgctccgga ggaaccggac
    16861 gacgcggaga tccggaagac gttcggagag atgaaggacc gggccgtcgc tcattacgaa
    16921 tcctacgccc tcaacaaggc cctggaggaa atctggagct ttatcacggc cgtcaacaag
    16981 tacctggccg acaaggcccc gtggaagctg gccaaggatc cggcgctccg gccgcagctg
    17041 gcccgggtgc tccatcaggc ggtcgcggcc atccgcggaa cggccgccct catcgccccg
    17101 gtcatgcccg gctcggccca gaagatgtgg gagttcaccg gggagcccgg ccgggtccgg
    17161 gacgtccgct ttgataccct gcgtttcgag gatttgccgg cggggaggcc gatgaaggag
    17221 cccgaacagc tctttccccg ggtgaatcta aaggagttcc tggccgaaga cgccgggacg
    17281 gcaaaggaaa aaaccatgaa cgagattcca tctccccccg aacgcccggc cgctccggct
    17341 gtttcggagg cgcccctcat cacgtacgac gaattcgcca agctggacct gagagtcgcc
    17401 catgtcctcg aggcggttaa ggtcgaggga gccaagaagc tcctgaagct caagatcgac
    17461 ctggggacgg agcagcggca gatcgtggcc ggagtggccg aatcctatgc cccggaacag
    17521 ctcgtgggga agaaattcat catcatcgcc aacctccagc ccgcggtcat ccggggagtc
    17581 gaatcgcaag gcatgctgct ggcggccgcc gccggcggca aggcgatgat tccgtttttc
    17641 gagccggacg tgccggccgg aacgaaggtg aaataggccg cctcaggcga gaaaaggcgc
    17701 gagacattcg accgggatcg ccccttcgcg gatgatcttc ggcggcgaca tggttacgtc
    17761 gacgatcgtg ctcgccaatc cgccgggggt ccgcccggca tcaatgatcg tgacgtcccg
    17821 attcttgaaa tcccgcgtca gcatgagcgg atcggcgttt tcgctgttgc ccgacaggtt
    17881 ggcgctcgtg gacgtcaggg gaacccccag gtcggctaaa aactcccgga gccaggacag
    17941 cccgggaacg cggacggcga tcgtttcgtc cggcccgagc atttccggcg gaaacaccgg
    18001 gcgggcatgg acgacgaggt tcaaggggcc cggccaaaac tgccggatca aatcgaagaa
    18061 gacgcgggga aggcggcggc cgagggaaag aacggcgccc gcgtccgcgg ccacgatcgg
    18121 caggggcttt ttccggtcgc gatgcttgag cgcgtaaacc gccttcaccg cctcggccga
    18181 aaacgcgttc gccccca
//
LOCUS       DAOOXH010000049        17297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_3197_length_17297_cov_9.107876, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000049 DAOOXH010000000
VERSION     DAOOXH010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 17297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17297
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3197_length_17297_cov_9.107876"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /gene="nadD"
                     /locus_tag="PKV64_07005"
     CDS             complement(<1..505)
                     /gene="nadD"
                     /locus_tag="PKV64_07005"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HPN16866.1"
                     /translation="MTSARRVGIFGGTFDPVHNGHLQAAAAVRSRFKLDRVLFIPSRI
                     PPHKRRSDMAPAAARLAMVEAAVRGTKGFSASPLEVRSPRTSYSIHTLAKIRKQMPAA
                     RLFFILGADAFGEIKTWREWRRVLGQCIFIVTTRPGTSLRAARAVLMPEYAVRMAAVR
                     KTSDIEAL"
     gene            complement(502..1542)
                     /gene="obgE"
                     /locus_tag="PKV64_07010"
     CDS             complement(502..1542)
                     /gene="obgE"
                     /locus_tag="PKV64_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPN16867.1"
                     /translation="MFIDQVKVYLKAGRGGDGAVSFRREKGVPKGGPDGGHGGHGGHV
                     LLVAEKNLATLAYYRFHPVNRAKNGEPGARARKQGKRGANLELRVPVGTVVKDAVTGR
                     ILCDLIEPGQGYIAAKGGKGGRGNTSFATSTHQAPREHEPGRPGEERELILELKMIAD
                     VGLIGFPNVGKSTLISKISAARPVIADYPFTTLIPNLGVVDVRTGRSFIVADIPGLIE
                     GAHRGQGLGIQFLKHVERTRILVHLIDVSPYSGRDPIEDYWIVRKEIAAFDPALAARP
                     QYIAANKIDLLREEDAGRLAAVKKLAAAEKCRFFAVSAMKKEGLDRLVRALDEAVARL
                     EAESSASRGNQR"
     gene            complement(1611..1865)
                     /gene="rpmA"
                     /locus_tag="PKV64_07015"
     CDS             complement(1611..1865)
                     /gene="rpmA"
                     /locus_tag="PKV64_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPN16868.1"
                     /translation="MATKRGSGVGRNGRDSAGRRLGVKKYGGEMVKAGSILVRQRGTP
                     FKPGPNVGRGQDDTLYAKISGVVRFSDKGERGKIVSVHPV"
     gene            complement(1878..2447)
                     /gene="rplU"
                     /locus_tag="PKV64_07020"
     CDS             complement(1878..2447)
                     /gene="rplU"
                     /locus_tag="PKV64_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPN16869.1"
                     /translation="MFAVIKTGGKQYVVKEGDELHVEKLATGAGGSVLFEEVLLIDDG
                     ENTLVGAPVVGKAVVKADVLETLRDDKVIVFKKKRRKQYRRTRGHRQFLAKIRIARIY
                     PDRTVVPAEELAVVAAPIVVSESVEPKAKPKTKAKAEKPAPKTAEGKPAAVKPAAKKP
                     AADKAAKPAKPKKTAETGKPKSRAKKAEK"
     gene            2772..2844
                     /locus_tag="PKV64_07025"
     tRNA            2772..2844
                     /locus_tag="PKV64_07025"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2805..2807,aa:Val,seq:tac)
     gene            3040..3915
                     /locus_tag="PKV64_07030"
     CDS             3040..3915
                     /locus_tag="PKV64_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN16870.1"
                     /translation="MAKARILIVEDEFLVAHDISNMLIELGYEVAAIVSTAEDALAVI
                     RNRAPDLILLDIRLKGPIDGIQAANIIKEEWSIPFIYLTAHADDLTLARAKSSDPLGY
                     LLKPFEFRELKTVIELAFYKYEKERQAAFWAIHDRLSGLPNKFLFLDHLSKAIYLGDR
                     HHKRIAILSFVVLPAGGSAGAPAPGLHDIVPEVTERLTRILRKSDTLARMESGEFIIL
                     LPDLETEEQSRIAARRILGVFDDPVDHEGRAVPVLVFYGLAMTDRTGQDPETLLQKSL
                     ANLAENLKINAGVSP"
     gene            3912..5255
                     /locus_tag="PKV64_07035"
     CDS             3912..5255
                     /locus_tag="PKV64_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPN16871.1"
                     /translation="MKNLQIAVVEDESLVAKDIVNMVRGLGYSVPAVVSTGEDAILIA
                     EKTRPDIILMDIVLKGRIDGIEAAHRIWETLSIPVVYLTAFADEATLQRAKVTEPFGY
                     ILKPFDERELQTTIEMAFYKAQMEKKLREREQWLTTILRSIGDGVIVTDADGRVTFMN
                     GVAERLTGWSQAEVLQRSLDGFIQIPIAPDPASDAPETAAGEGVLTMRDGISIPIEQS
                     VSPIFGEKKDVFGSVYIFRDIRSRKKAEDELRQSYLRHQKALAGTIQAIGLTIEMRDP
                     YTAGHQRRVSKLSCAIAEEMGLDPARIEGVRMAGDIHDIGKIYVPAEILSKPGRLSDI
                     EMSIIRPHPQVGYDILKNIEFPWPISQIIYQHHERLDGSGYPNRLKAEAILLEARIIS
                     VADVVEAMSSHRPYRPAPGIEKALEEIQRGRGVQYDREVVDHCLRLFLEKKFVFE"
     gene            5256..5492
                     /locus_tag="PKV64_07040"
     CDS             5256..5492
                     /locus_tag="PKV64_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16872.1"
                     /translation="MRKGLSVFLIVTAAGLSLAGIIGFLFLILPRMNAFWIVLAPVIF
                     AVYQIPAVAVYALWKRGRTEDRAGQKNPPPPEES"
     gene            complement(5593..5665)
                     /locus_tag="PKV64_07045"
     tRNA            complement(5593..5665)
                     /locus_tag="PKV64_07045"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5629..5631),aa:Glu,seq:ttc)
     gene            complement(5677..5749)
                     /locus_tag="PKV64_07050"
     tRNA            complement(5677..5749)
                     /locus_tag="PKV64_07050"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5714..5716),aa:Lys,seq:ttt)
     gene            complement(5874..6563)
                     /locus_tag="PKV64_07055"
     CDS             complement(5874..6563)
                     /locus_tag="PKV64_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16873.1"
                     /translation="MKKNGLIILVICLAVIAAQSCKSHPEEALLKSYFSASSMNDVAT
                     MSSMAFDPMKIDAVSWKIVQVSEEKVTPAALAEMNQKELDLKKQFEASSGPALDAQDA
                     LNMAKDEYTAARTPAARAAAKKKQEAAQLKYDEAYKANRDLVKAYNDAKEAAAREEEK
                     MAFSLGIKDLVNIRELKGDVASKDIQVEVQTKDGQTKNLKIHMEKYTMKDEAAGLAHR
                     GRWVLTKFENL"
     gene            6734..8557
                     /locus_tag="PKV64_07060"
     CDS             6734..8557
                     /locus_tag="PKV64_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPN16874.1"
                     /translation="MPAEGSLASILARHRRDRTRLIDILREVQAKNGHISPEAAAEIA
                     AGLGLSTVEVEGAATFYHFFSARPAGTYAVYLNDNIVSVMKGRAAVASAFEKAAGCRF
                     GETTPDGRIGLYRTSCIGMNDQEPAALINGVVFTKLTPAKVKLIVEGMNAGMNVRAMI
                     AATGDGANAAKLVRAMVKNNIRKEGPVILASFRSGAAVKKAVKITPEGVIAEVKKANL
                     LGRGGAGFPTGLKWEFCRREKGENHYVVCNADEGEPGTFKDRVILTERPHLLFEGMTV
                     AGYAVGARKGLLYLRGEYAYLAPYLESLLAGLRKKNLLGKRIAGKAGFSFDIEIKLGA
                     GAYVCGEESALLQSAQGERGEPRDRPPFPVQSGFLFQPTTVNNVETLCAAARIIEKGA
                     DWFKAMGTPRSAGTKVLSVSGDCKKPGVYEVPFGLTVRRLLEMAGGPDAQAVQIGGPS
                     GACISRGQLERAIAFEDLATGGSIIVIGPGRDLFEVIRNFMEFFVEESCGWCVPCRAG
                     NPLLLRKLEKIMSGKGTPADIIEIESWGRIIKAMSRCGLGQTSPNPILTTIQNFREMY
                     ESKVERGRDFLPEFDLAEAVKEASAVTGQVFAASDTEQSHE"
     gene            8550..9263
                     /locus_tag="PKV64_07065"
     CDS             8550..9263
                     /locus_tag="PKV64_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPN16875.1"
                     /translation="MSKPKARISFTVDGKKCSAAPGQTILQAAKDNGIYIPALCRWEG
                     FKPAGACRVCTVRVGGRYMAACTQPVTDGMAVENDVPDLQDMRKALIEMLFVEGNHMC
                     PTCEKSGNCELQALAYRFLMLVPRFPFQFPARRVEPAGKKILLEHNRCIKCLRCVRGV
                     RTRDNKAVFGTLRRSRDKKISVDQVLASALTDKQARQAMDRCPVGSILKKEVGFAVPI
                     GRRKYDKEPIGSDVEKARG"
     gene            9274..9822
                     /locus_tag="PKV64_07070"
     CDS             9274..9822
                     /locus_tag="PKV64_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HPN16876.1"
                     /translation="MSKPVVATASLAGCFGCHMSFLDIDERILTLIDLVEFNKSPIDD
                     IKTFTKPVDIGIIEGGCCNSENVEVLREFRKHCQVLISLGECAIMGGLPAMRNAIPVR
                     ECLEEAYFHSPTIDPSEPKIMPGDEDLPIILDKVLPLHEIVKIDAFLPGCPPSADLIW
                     KALTSLLEGKPLDLSYNLVKFD"
     gene            9833..11281
                     /locus_tag="PKV64_07075"
     CDS             9833..11281
                     /locus_tag="PKV64_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HPN16877.1"
                     /translation="MARKIVIEPVTRVEGHGKVTIRLDDRNNVASSRLHIVEFRGFER
                     FVQGRPYWEAPVLVQRLCGICPVSHHLAAAKAMDQVIGVEADGLSPTAEKMRRLMHYG
                     QMFQSHALHFFHLAAPDLLFGADADPAVRNVIGIALTNKELATRAVLLRKYGQEVILA
                     TAGKKIHGTGAVPGGINKNLSIEERDALLQGAGAPAVETMIDWAEEAVEFLKDYHAKN
                     RALMDGFAAFPSNHLGLVGRDGALDFYHGAMRAVDAGGKRILDDVDYRDYLKYIAEEV
                     RTWSYMKFPYLKSLGKAEGWYRVGPLARLNVCDFIPTPRAQKQFEAFRAYTGGKPNNM
                     SLHMHWARLIELLHSGEVIRELLLDPDLQKTELVRKGRKRGEGVGLVEAPRGTLFHHY
                     RVDENDQISMANLIVSTTNNNEPMNRAVHWVAMNVLSGKRDITEGMLNRVEIAIRAYD
                     PCLSCATHALGRMPLAVELYDAGGNLIDRKAR"
     gene            11278..11757
                     /locus_tag="PKV64_07080"
     CDS             11278..11757
                     /locus_tag="PKV64_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HPN16878.1"
                     /translation="MKTLLIGIGNPGRGDDGLGPALVERLTGNRPPERTVIPAAGGSV
                     DAYWTYQLNIEDASLLREYGRVVFADAAVDAAAPAELLPLDPAASIAFTSHEMSPASV
                     LALAEELYGGSPAARLLRIGGAEWGFVERLSAEAGRHLDEAERLFWEFAGPARTITR"
     gene            complement(11747..13165)
                     /locus_tag="PKV64_07085"
     CDS             complement(11747..13165)
                     /locus_tag="PKV64_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013925816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HPN16879.1"
                     /translation="MFKKLWTRLGFLPRKRVSVVIPVLNEEKTIGQVVRLAKKDKTVG
                     EVIVVDDNSVDQTVAEAEKSGAAVIKSSVLGKGYSMGEGLDLAKFEVVVFLDGDIGNY
                     ETDIVRRLSEPVIRDWCDFAKGTFRREAGRVTELVAKPLLSILFPDLSGYSQPLSGMI
                     AAKKEFLKGLKFENDYGVDIGILIDVHNRGARIMEVDLGRIDNKSKPWQALGKMSREV
                     SKAILKRAEIKKILSLDELETINVIRDEMEDSINDSIQKLNKMILFDMDDTLLLERFI
                     NKAADAFGFRAKLEEIRANNSEPFVVTKLIAALLKDVKLEEIFEIADGLQIVPDAAEV
                     IKILKSRGYVVGLVTDSYDLIADFIRNKVGADFALANQLEVKNRTVTGEVRIPSYFIR
                     GEKSPCSHAVCKVNALVYLSEKHGLTTADMIAVGDSVNDLCMVKAAGIGVAFCSKNGL
                     LNVIADKVISTRSFRDLLDLTA"
     gene            13328..13996
                     /gene="hypB"
                     /locus_tag="PKV64_07090"
     CDS             13328..13996
                     /gene="hypB"
                     /locus_tag="PKV64_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HPN16880.1"
                     /translation="MAKKDVFVLQDILLSNAQTAAAIRRELESRKILAVNLLSSPGAG
                     KTTLLEALAERLKGRARMAVIEGDIETERDAERIRARGIPAWQITTGGACHLEAKMIG
                     KVLPDIPTDIDFLFIENVGNLVCPAGYDLGERLRMVLLSAPEGDDKPKKYPLAFVTAD
                     VLLLSKSDLRPFLPFDAEKAQAEALALNPKLRVFIISAVSGQGLDDLVDFLFRAREEI
                     ASRA"
     gene            13989..14354
                     /gene="hypA"
                     /locus_tag="PKV64_07095"
     CDS             13989..14354
                     /gene="hypA"
                     /locus_tag="PKV64_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HPN16881.1"
                     /translation="MHELSLVASMFETLLEQARLHKARRITGVRIQVGALAGVVPELL
                     ESAFDSYKKGTIADGAVLTIVKPPFTIRCRACGDETVREDFALACPACASTDLEIVHG
                     MELVLEKIEMETDDPLPAP"
     gene            complement(14427..16463)
                     /locus_tag="PKV64_07100"
     CDS             complement(14427..16463)
                     /locus_tag="PKV64_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HPN16882.1"
                     /translation="MNKGTVWGGLRTGVAAGLAAGLVDALFAVAQSGGGLAGSSLAAF
                     LISLSLWGMIGGLGTAAVGAVFPEAGGDAPARQKTTVISFCKKALFDFVLTVLFVSAA
                     GLLAGRTAILVSGAKDFAGLAAGVAATAFVLSAAGIATWRQPPGRRTRFLRIAGFAGL
                     GLVLIIIALDRGGTRAGKIDAGGGAPAGRPAPPAGSPSVLLIVMDTARADHVSLREGE
                     RETMPFLRRLAAESAVYERAVSPSPWTVPAHASLFTGQYAEVHQATFEHRTLRPELET
                     MAELLRSAGYSTAAFSSNPNVSSLFHFDQGFDRFYEMFRLSGLWDARGLDPVENLKIL
                     KGLGAALGSPRKKRSDRAAAETQPWIARWLDSQNRRDKNRPFFIFVNYMPPHLPYNPP
                     PAYRRRFLKNRPSPPVRKLMTDACYPEVWRLIAFPTLMRPADYGRLAELYDGELAFLD
                     SCLERLIAMFRERGLLEETVVIITSDHGENLGEHGGLLNHSFSIHQTLLRVPLIVRYP
                     RVFGKNKRYAGVVSTGSIFPTVLKLAGVSPADNWPSAFGLLPDAGPTAGIAPEEEAAV
                     SEYELPLSELGALAELPGADASAFSLSQKAVQTDSWKLVSRSDGRSFLYNLAADPGEE
                     IPLDPRSSPQGARLERLLEHWRAGLHIPHFSPEPPGPTADEKTRETLRSLGYLR"
     gene            complement(16519..17280)
                     /locus_tag="PKV64_07105"
     CDS             complement(16519..17280)
                     /locus_tag="PKV64_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HPN16883.1"
                     /translation="MNAKTHISIKNLTVTFGRQKVLKDVSVDLPDHRVTAIIGPSGCG
                     KTTLLKTLNRLLDKVDGVKVEGQVLVDGENIYDPKIEVTRIRKKMGLLSQKPQVLPMS
                     IFDNIAYGPRIHGVREKAKLRQIVERQLAAAGLWDEVKTRLHEPASRLSVGQQQRLCL
                     ARGLAVEPEIILGDEPTSALDPQSSRTVEQRLIDLKTDYTVIVVTHILRQARRIADYI
                     VFMYLGELVEHGPAAEVFAEPKDPRMKAYLTGEIS"
BASE COUNT         3384 a         5525 c         5177 g         3211 t
ORIGIN      
        1 ggagagcctc gatatcgctc gtcttccgaa ccgcggccat gcggaccgcg tattcgggca
       61 tcaggaccgc ccgggccgcc cgtaggctgg ttcccggccg ggtcgtgacg atgaaaatgc
      121 attggccgag cacgcggcgc cattcccgcc aggtcttgat ctcgccaaag gcgtcggctc
      181 ccaggatgaa aaacagccgg gcggccggca tctgcttccg gattttcgcc agggtatgga
      241 tcgagtagga cgttcgcggc gaacggacct ccaaagggct ggcgctgaat cccttcgtcc
      301 cccggacggc ggcctcgacc attgccaggc gggcggcggc cggagccata tcggaacggc
      361 gcttgtgcgg cggaatgcgc gagggaatga aaagcacgcg gtccagtttg aagcgcgatc
      421 gaacggccgc ggccgcctga agatggccgt tatggacggg atcgaaggtc cccccgaaga
      481 ttccgactcg ccgagcggag gtcacctttg atttcctcgg gacgcggagg actccgcctc
      541 caggcgggcg accgcttcgt ccagggctcg gacaagccgg tccagcccct ctttcttcat
      601 cgccgagacg gcgaagaacc ggcatttttc ggctgcggcg agtttcttga ccgccgccag
      661 gcggccggcg tcttcctcgc ggagcaggtc gatcttgttg gccgcgatgt actgcggccg
      721 ggcggccagg gccgggtcaa aggccgcgat ttccttccgg acgatccaat aatcctcgat
      781 cggatcgcgg ccggaatagg gcgaaacatc gatcaagtgg acgagaatcc gggtgcgctc
      841 gacgtgcttg agaaactgaa tgcccagccc ttggccgcga tgggcccctt cgatcaagcc
      901 ggggatgtcg gcgacgatga agctccgtcc ggttcggaca tccacgaccc cgagattcgg
      961 aatcagcgtc gtgaagggat agtcggcgat gaccgggcgg gccgcggaga ttttcgaaat
     1021 cagggtggat ttcccgacat tggggaatcc gatcagcccc acgtcggcga tcatcttgag
     1081 ctcgaggatc aactcccgct cctcgcccgg acggccgggc tcgtgctccc gcggggcctg
     1141 atgggtggat gtggcgaagg aagtgttgcc gcgcccgccc ttgcctccct tggcggcgat
     1201 atatccctgg ccgggttcaa tcaggtcgca caggatcctt cccgtcacgg cgtccttgac
     1261 gacggttccg acgggaacac gaagctcgag gttcgccccc cgtttcccct gtttgcgggc
     1321 ccgcgctcca ggttcgccgt tcttcgcccg gttgaccgga tggaatcggt aatacgccag
     1381 ggtcgccaga tttttttccg ccaccaaaag aacatggccg ccgtgaccgc cgtgaccgcc
     1441 atcggggcct cccttcggga cgcccttctc gcgccggaag ctgaccgcgc cgtcgcctcc
     1501 ccggccggct ttcaaataaa ccttgacctg gtcgatgaac atgaacggtt tagagaaaag
     1561 acatcccccg cctcacggaa aacgaggaat cgagatagag ccgatgaacg ttaaacgggg
     1621 tggaccgaga caattttgcc cctctcgccc ttatcggaaa accggaccac gccggaaatc
     1681 ttggcgtaaa gggtgtcgtc ctgcccgcgg cccacgttgg ggccgggctt gaacggagtc
     1741 ccgcgctgcc ggaccaggat cgatcccgcc ttgaccatct cgccgccgta ttttttcacg
     1801 ccgagccgcc ggcccgcgct gtcccggccg ttccgcccga ctccgcttcc tcgttttgtg
     1861 gccatggatc gtctccttta cttctccgct ttcttggcgc gggatttcgg tttaccggtc
     1921 tcggcggttt tcttgggttt cgcgggcttg gcggccttat cggccgcggg cttcttggcc
     1981 gccggcttca cggcggccgg ttttccctcg gccgtcttgg gcgccggctt ttcagctttc
     2041 gccttggttt tgggcttggc cttgggctcg acggactcgg agacaacgat cggggccgcg
     2101 acgacagcca gctcttcggc cggaacaacc gtccgatcgg ggtaaatccg ggcaatccga
     2161 atcttggcca ggaattgacg atgcccccgg gtccgacggt attgtttacg gcgtttcttt
     2221 ttaaaaacaa tcactttatc atcccggagc gtctccagga catccgcctt cacgaccgcc
     2281 ttcccgacaa ccggcgctcc gacaagggtg ttttccccgt catcgatcag gaggacttct
     2341 tcgaaaagaa cggatcctcc ggcgcccgtg gcgagctttt cgacatgcag ctcatcgcct
     2401 tccttgacga catactgttt cccgccggtt ttaatgactg caaacatggt agttttcctc
     2461 gaattgaagt caaaaatccg caaaaaggag cataacttta acatgggctt gcgtttatgt
     2521 caaacggctt ttctttcccg gggaaaagac ttccctcgga acgcggcgtc cggcaaaatc
     2581 cggcgcggac ggccccgcct gaccgggagc ggccaggcat atccgcgtct cgagaggtct
     2641 ctcccgcaaa gggtggaaaa atgccgtctt caattgaatt tgaggaggcc tgatcgtccg
     2701 aaacacgctt gccgggttca tcggcccgcc ccgggagagg aggaaaatga tgtataatca
     2761 acggacggaa cgggcggtta gctcagccgg tagagcatcg gtcttacacg ccggtggccg
     2821 cgggttcgaa tcctgcaccg cccatcctcc cccccgcaca tcattcatgc cggaccgagg
     2881 aggggggctc caagacccgg aaacggcggc tgccgggcaa aaagtcgggg aaaacgcccg
     2941 gtctccagga aaagccggcg gcgccggaaa gggacgtcgg gggcgttctt ttgacatccc
     3001 cccccgggga tggtataaaa tcctgcgggg aggagagtcg tggccaaagc cagaatcctc
     3061 atcgtcgaag acgaatttct cgtcgcccac gatatcagca acatgctgat cgagctgggt
     3121 tacgaagtcg cggcgatcgt atcgacggcg gaagacgccc tcgccgtgat ccgcaaccgc
     3181 gctcccgacc tcatcctcct ggatatccgg ctcaagggac cgatcgacgg catccaggcc
     3241 gccaatatca tcaaggagga atggtcgatc cccttcatct acctgacggc ccacgccgat
     3301 gatctaaccc tcgcccgggc caagagctcc gatcccttgg gatacctgct caagccgttc
     3361 gaattccgcg agctcaaaac ggtcatcgag ctggcttttt acaagtatga aaaagagcgc
     3421 caggccgctt tctgggccat ccacgaccgt ctgtccggcc tgccgaacaa gtttctcttc
     3481 ctggaccatt taagcaaagc gatttacctc ggcgaccgcc accataaaag aatcgccatc
     3541 ctgtcctttg ttgtcctccc ggccggcggc tccgccggag ccccggcgcc cggcctgcac
     3601 gacatcgtcc ccgaggtgac ggaacggctc acgcgcattc tccgcaagag cgacacgctg
     3661 gcccggatgg aaagcggaga attcatcatt cttctccccg acttggaaac cgaggagcag
     3721 tcgcggatcg ccgcccgccg gatcctgggc gttttcgatg atccggtcga ccacgagggc
     3781 cgggccgtgc cggtcctcgt tttctacggc ctggcgatga ccgaccggac cggacaggat
     3841 cctgaaaccc tgctccaaaa atccttggcg aatctggccg aaaacctgaa aatcaacgcc
     3901 ggcgtgagtc catgaaaaat ctccagatcg ccgtcgtcga agacgaaagc ctcgtcgcca
     3961 aagacatcgt caacatggtg cggggtctcg gctactccgt tccggcggtc gtatccaccg
     4021 gcgaggacgc gatcctcatc gccgaaaaaa cgcgccccga tatcattctc atggatatcg
     4081 tcctgaaagg gcggatcgac ggcatcgaag ccgcccaccg catctgggaa acgctgtcca
     4141 tcccggtcgt ctatctcacg gcgttcgccg acgaagcgac gctgcagcgg gccaaggtga
     4201 ccgagccgtt cggatacatc ctcaagccgt tcgacgaacg ggagcttcag acgacgatcg
     4261 aaatggcctt ttacaaggcc cagatggaaa agaagctgcg cgagcgcgag cagtggctca
     4321 cgacgatcct tcgatccatc ggggacgggg tgatcgtgac cgacgcggac ggccgcgtca
     4381 cgttcatgaa cggagtagcc gaaagactca cgggatggag ccaggcggag gttcttcaac
     4441 ggtcgttgga cggcttcatc cagatcccca tcgccccgga tccggcttcg gacgccccgg
     4501 agacggcggc cggcgagggg gtgctgacca tgcgggacgg aatctccatc ccgatcgaac
     4561 aatcggtctc ccccattttt ggggagaaaa aggatgtctt cggctccgtt tacatcttcc
     4621 gggatatccg gagccggaag aaagccgagg acgagctccg gcaaagctat ctccgccatc
     4681 aaaaagccct ggccggaacc atccaggcga tcggcctgac catcgaaatg cgcgacccgt
     4741 acacggccgg ccaccagcgc cgggtcagca agctgtcctg cgcgatcgcc gaggaaatgg
     4801 gactggatcc cgcccggatc gagggcgtcc gcatggccgg cgacatccat gacatcggaa
     4861 agatctacgt tccggccgaa attttaagca agcccggccg gctgagcgac atcgagatgt
     4921 cgatcatccg cccccaccct caggtcgggt acgacatcct caagaacatc gaattcccct
     4981 ggccgatctc ccagatcata taccagcatc acgaacggct ggacggatcg ggctacccga
     5041 accgcctcaa ggcagaagcg attctgctcg aagcccggat catcagcgtc gccgacgtcg
     5101 tcgaggccat gtcctcgcac cgcccctacc ggccggcccc gggcatcgaa aaggcgctag
     5161 aagaaataca gcgcggccgc ggcgtccagt acgaccggga ggtcgtcgac cattgtcttc
     5221 gcctcttcct ggaaaagaag ttcgtctttg aataaatgcg caagggcttg tccgttttct
     5281 tgatcgtcac ggccgcaggg ctgtccctgg ccggaatcat cgggttcctc ttcctcattc
     5341 ttccccggat gaacgcgttc tggatcgtcc tcgccccggt gattttcgcc gtttaccaga
     5401 tacccgccgt cgccgtctat gccctctgga agcgagggcg gacggaagat cgggccggac
     5461 agaaaaatcc tccgccgccg gaggagtctt aaccggcgcg gaaaacacgg tcgagcggcg
     5521 aggaagggga agaaccgccg ggacggccgg gcctggcgag aggcttcctt tgagattttt
     5581 ttaacaggaa tacgcccccg acgggattcg aacccgtgtt gctgccgtga aagggcggtg
     5641 tcctgggcct ctagacgaca ggggcgcaaa gcattatgag ccgagcggga ttcgaacccg
     5701 cgacacccgc cttaaaaggg cggtgctcta ccagctgagc tatcggcccg ccgaagagac
     5761 gcatatttat agccgaatcc cccgggagag tcaagaaaat ccgctctgga aacggaaatc
     5821 cccctgtccc cgacggaaca gggggatgga gctcgttaca aagaggcttt aactcagagg
     5881 ttctcgaact tcgtgaggac ccagcgccca cgatgagcca agccggccgc ctcatccttc
     5941 atcgtgtatt tttccatgtg gatcttgagg ttcttggtct ggccgtcctt tgtttggact
     6001 tccacctgga tatccttgga cgcgacatcg cctttcagct cgcggatgtt gaccaaatcc
     6061 ttgatcccga gcgagaaggc cattttctct tcttcccgcg cggccgcttc cttggcatcg
     6121 ttgtacgctt tcaccaaatc ccggttggcc ttgtacgcct catcgtattt gagctgggcc
     6181 gcttcttgtt ttttcttggc agcggcccgg gcggcgggcg tccgggcggc cgtgtactcg
     6241 tctttcgcca tattcagcgc gtcttgggcg tcaagggcgg gccccgaact ggcctcgaac
     6301 tgctttttca ggtcgagctc tttctgattc atctccgcca gcgcggccgg agtgactttt
     6361 tcctcgctga cctggacgat cttccagctg acggcgtcga ttttcatcgg atcgaacgcc
     6421 atcgacgaca tcgtcgcgac atcgttcatg gagctggcgc tgaagtaact cttcaacaag
     6481 gcttcttccg gatgggattt gcagctttga gccgcgatga cagccaggca aatcaccagg
     6541 atgattaagc cgttcttctt cattcctcct cctcctgtcg gacgatataa taagaacact
     6601 acttcgaatt ctagcccatt tgacgaagaa aaggcaagcc cccttcaaaa caaaaacgcc
     6661 gattgagccc ggcggatgat tgtgttataa aaaagcttcc tgttttaaga atcctttcgc
     6721 aggagaggtt ctcatgccgg cagaaggatc cctcgcttcc attctcgccc gtcaccgccg
     6781 ggaccggacc cgcttgatcg acatcctccg ggaagttcag gctaaaaacg ggcatatttc
     6841 gcccgaagcc gcggccgaaa tcgccgccgg cctggggctc tccacggtgg aggtcgaagg
     6901 ggcggccacg ttctatcatt tcttttccgc ccgtcccgcc gggacctacg ccgtttatct
     6961 caacgacaac atcgtctcgg tcatgaaggg ccgggcggcc gtcgcctcgg cttttgaaaa
     7021 agcggccgga tgccggttcg gtgaaacaac gcccgacggc cggatcgggc tttaccggac
     7081 ctcctgcatc gggatgaatg atcaggagcc cgcggctctc atcaacggcg ttgtgttcac
     7141 gaaattgacg ccggccaagg tgaagctgat cgtcgaaggg atgaacgcgg gcatgaacgt
     7201 ccgggcgatg atcgccgcga cgggggatgg ggccaacgcc gccaagctcg tccgggcgat
     7261 ggtcaaaaac aacatccgca aggaaggccc ggtcatcctc gcttccttcc ggtccggcgc
     7321 ggccgtcaag aaagcggtga aaatcacgcc cgagggcgtc atcgccgaag tcaagaaagc
     7381 caacctgctg ggccgcgggg gggcgggatt cccgacggga ttgaaatggg agttctgccg
     7441 gcgggaaaag ggagagaatc actacgtcgt ctgcaacgcc gacgaaggcg aacccgggac
     7501 attcaaggac cgggtcatcc tgaccgagcg cccccatctc ttgttcgagg gcatgaccgt
     7561 ggccggctac gcggtcgggg cccggaaagg cctcctctac ctccggggcg aatacgccta
     7621 cctcgcgccg tatcttgaaa gcctcctggc cgggctccgc aagaaaaatc tcctggggaa
     7681 aaggatcgcc ggcaaggcgg gcttctcctt cgacatcgag atcaagctcg gggccggagc
     7741 ctatgtctgc ggcgaggaat cagctctcct ccaatccgcc caaggcgaac ggggcgaacc
     7801 gcgcgaccgg cctcccttcc ccgtccaatc cggcttcctg ttccagccga ccacggtcaa
     7861 caacgtggaa acgctgtgcg cggccgcccg catcatcgag aagggagcgg actggttcaa
     7921 ggcgatggga acgccccgtt cggccggaac caaggttctg agcgtgtcgg gcgactgcaa
     7981 gaagcccggc gtctatgaag tgccgttcgg cctgaccgtt cgccggcttc tcgaaatggc
     8041 cggaggcccc gacgcccagg ctgttcagat cggcggtcct tcgggcgctt gcatctcccg
     8101 gggacaactg gaacgggcga tcgccttcga ggatttggcc acgggagggt cgatcatcgt
     8161 catcgggcca ggccgggatc ttttcgaagt catccgcaat ttcatggagt tcttcgtcga
     8221 ggagtcctgc ggctggtgcg tcccgtgtcg ggcgggcaac cctctcctcc tgcgcaagct
     8281 cgaaaagatc atgtcgggca agggaacccc ggcggatatc atcgaaatcg aatcctgggg
     8341 ccggatcatc aaagccatga gccggtgcgg cctcggacag acctcgccca acccgatcct
     8401 gaccaccatc cagaacttcc gggagatgta cgaatccaaa gtcgagcggg gccgcgactt
     8461 cctgcccgaa ttcgatctgg ccgaagccgt caaggaagcg tcggccgtca ccggccaggt
     8521 tttcgccgct tcggacacgg agcaatccca tgagtaagcc caaagctcgg atttcattca
     8581 ccgtcgacgg caagaaatgc tcggcggcgc ccggccagac cattcttcag gcggccaaag
     8641 acaacgggat ctacatcccg gccctctgcc gctgggaggg atttaagccg gccggcgcct
     8701 gccgcgtctg caccgtccgc gtcggcggcc gctacatggc cgcctgcacc cagccggtga
     8761 cggacgggat ggctgtcgaa aacgacgtcc cggacctcca ggacatgcgg aaggccctga
     8821 tcgagatgct cttcgtcgag ggcaaccaca tgtgcccgac ctgcgaaaaa agcggcaact
     8881 gcgagcttca agccctggcg taccgctttt tgatgctcgt cccccggttt cctttccagt
     8941 tcccggcccg ccgcgtcgaa cccgccggga aaaagatcct tctcgagcat aaccgctgca
     9001 tcaaatgcct tcgctgcgtc cgcggcgtcc ggacccggga caacaaggcc gtctttggga
     9061 cgctgcgtcg ttcccgcgac aaaaaaatct ccgtggacca agtcctggcc tccgccttga
     9121 cggacaaaca ggcccggcag gccatggacc ggtgcccggt cggctccatc ctgaagaagg
     9181 aagtcggctt cgccgtcccc atcggccggc ggaagtacga caaggaacca atcggcagcg
     9241 acgtcgaaaa agcccgcggc tgaggagaat tccatgagca aacccgtcgt cgccacggcc
     9301 tcgctggccg gatgcttcgg ctgccacatg tcttttttgg acatcgacga gcggattctc
     9361 actctcatcg acctcgtcga gttcaacaaa tcccccatcg acgacatcaa gacattcacc
     9421 aaacccgtgg acatcgggat catcgagggc ggatgctgca acagcgaaaa cgtcgaagtg
     9481 ctccgcgagt tccgaaagca ctgccaggtc ctgatttctc tcggggaatg cgccatcatg
     9541 ggcggtcttc cggcgatgcg gaacgccatc ccggtccgcg aatgcctgga ggaagcctat
     9601 ttccactcgc cgacgatcga tccgtccgag ccgaaaatca tgcccggcga cgaagacctc
     9661 ccgatcatcc tggacaaagt ccttcccctt catgaaatcg tgaagatcga cgccttcctt
     9721 ccgggctgcc ccccttcggc cgacttgatc tggaaggccc tgacctcgct gctcgagggc
     9781 aagccgctcg atctgagcta caacctcgtc aagttcgact gagaggaaca ccatggcgcg
     9841 caaaatcgtc atcgaacccg tcacccgagt cgaaggccac ggcaaggtca cgatccgcct
     9901 cgacgaccgg aacaacgtcg cttcgagccg gcttcacatc gtcgagttcc gcggcttcga
     9961 gcgcttcgtc cagggccggc cgtactggga agcgccggtc ctcgtccagc ggctctgcgg
    10021 catctgcccc gtcagccatc acttggccgc ggccaaggcg atggaccagg tcatcggcgt
    10081 ggaggccgac ggtctctcgc ccacggccga aaagatgcgg cggctcatgc attacggaca
    10141 gatgttccag tcccacgcgc ttcacttttt ccacctggcc gcgcccgacc tgctctttgg
    10201 agccgacgcc gatccggccg tccgcaacgt catcggcatc gctctgacga ataaggagct
    10261 ggccacccgg gccgtgctgc ttcggaagta cggccaggaa gtcatcctgg ccacggccgg
    10321 aaagaaaatc cacggaaccg gggccgttcc cggcggaatc aacaagaacc tgtccatcga
    10381 ggagcgggac gcccttctcc aaggagccgg cgcccccgcc gtcgagacga tgatcgattg
    10441 ggccgaggag gccgtcgagt tcctcaagga ctaccacgcc aaaaaccggg ccctcatgga
    10501 cggcttcgcc gcctttcctt cgaatcacct cggcctcgtc ggccgggacg gcgccctcga
    10561 tttctaccat ggggcgatgc gggccgtgga cgccggggga aaacgaatcc tcgacgacgt
    10621 cgactaccgg gactacctga aatatatcgc cgaggaagtc cggacctggt cctacatgaa
    10681 gttcccctac ctgaaatccc tcggcaaggc tgaaggctgg taccgggtcg ggcccctagc
    10741 ccggctcaac gtctgcgact tcatcccgac gccgcgggcc cagaagcagt tcgaggcctt
    10801 ccgggcgtat accggcggca agcccaacaa catgtccctc catatgcatt gggcccggct
    10861 gatcgagctt ctccacagcg gcgaagtcat ccgggagctc ctcctcgacc cggacctgca
    10921 gaaaaccgaa ctcgtccgca aaggcaggaa acggggcgag ggagtgggcc tcgtggaagc
    10981 cccccggggg acgctgttcc accactaccg ggtggacgaa aacgatcaaa tctccatggc
    11041 caacctgatc gtctcgacga ccaacaacaa cgagcccatg aaccgggccg tccactgggt
    11101 ggccatgaac gtcctcagcg ggaaaaggga tatcaccgag ggcatgctca accgggttga
    11161 gatcgcgatc cgggcctatg atccctgtct gtcctgcgcc acccacgcgc tcggccggat
    11221 gcccctggcc gtcgagcttt acgacgcggg cggaaacctg atcgaccgca aggcgcggtg
    11281 aagacgcttc ttatcgggat cggcaatccc ggccggggag atgacggact cggcccggcc
    11341 ctcgtcgagc ggttgacggg aaaccgcccg ccggagagga ccgtcatccc cgccgccggt
    11401 ggatcggtcg acgcctactg gacataccag ctcaacatcg aggacgcttc ccttcttcgg
    11461 gaatacggcc gcgtcgtttt cgccgacgcg gccgtcgacg cggcggcccc ggccgagctt
    11521 ctccccctgg acccggcggc ttcgatcgcc ttcaccagcc acgagatgtc cccggcctcg
    11581 gtcctggccc tggccgagga actgtacggc ggatccccgg cggcccgtct tctccgcatc
    11641 ggcggggcgg agtggggctt cgtcgagcgg ctttcggccg aagccggacg gcatttggac
    11701 gaagcggaac ggctattctg ggaattcgcc ggtccggccc ggacgatcac gcggtaagat
    11761 ccaaaaggtc ccggaaagac cgggtggaaa tcactttatc cgcgatcacg ttcaacaacc
    11821 cgttctttga gcagaaggcg accccgattc ccgcggcctt gaccatgcag aggtcgttga
    11881 cgctgtcgcc gacggcgatc atgtcggccg tcgtgaggcc gtgtttctcc gaaagataaa
    11941 ccaaggcgtt gaccttgcag acggcgtgag agcaggggct cttttcgccg cggatgaaat
    12001 aagacgggat ccggacttcc ccggtgaccg tccggttttt aacctcgagc tggttggcca
    12061 gggcgaaatc ggccccgacc ttgttccgga tgaagtcggc gatcaggtcg tagctgtccg
    12121 tgacgaggcc gacgacatat cccctggatt tcaggatttt gatcacctcg gccgcgtccg
    12181 gcacgatctg gaggccgtcg gcgatctcga aaatctcttc cagcttcacg tccttgagga
    12241 gggcggcgat cagcttcgtc accacgaacg gctcgctgtt gttcgcccgg atctcctcga
    12301 gcttggcccg gaagccgaac gcgtcggcgg ctttgttgat gaaccgctcc aggaggaggg
    12361 tgtcgtccat atcgaacaag atcatcttgt tcagcttctg gatggagtcg ttgatcgagt
    12421 cctccatctc gtcgcggatg acgttgatgg tttccaactc gtccaggctc aggatcttct
    12481 tgatctcggc ccgcttcagg atcgcctttg aaacctcccg ggacatcttc cccagggcct
    12541 gccagggttt ggatttattg tcgatccgtc cgaggtcgac ttccatgatc cgggcgcccc
    12601 ggttgtggac gtcgatcagg atgccgatat ccacgccgta gtcgttctcg aatttcaagc
    12661 ccttcaggaa ttccttcttg gccgcgatca tcccgctcaa cggctgggaa tagccggaca
    12721 gatcggggaa gagaatgctc aggagcggct tggccacgag ctccgtcacc cggccggcct
    12781 cgcggcggaa ggtccccttg gcgaaatcgc accagtcccg gatcaccggt tcggacagcc
    12841 ggcggacgat gtcggtctcg taattgccga tgtccccgtc gagaaagacg acgacctcga
    12901 atttcgccag atccagccct tcccccatgg aatagccctt gcccagaacg gagcttttaa
    12961 tcacggcggc gccgcttttt tccgcctcgg cgacggtctg gtcgaccgag ttgtcgtcga
    13021 cgacgatcac ttccccgacg gtcttgtcct tcttcgccag ccggacgact tggccgatgg
    13081 ttttttcctc gttgaggacg gggatgacga cgctgacccg tttccgggga aggaatccga
    13141 gccgtgtcca aagtttttta aacatttcgt atccgacgcc gctccgcccc tcgggcggaa
    13201 aatccctttt ttttcatgat agggatttca tccgggaaat gcaacgattt tcaaaaacga
    13261 agaaatccac tagaattcaa aaggtttttt gaataatgcg ggtataatag ggccgaaagc
    13321 gaatcccatg gccaagaaag acgtcttcgt cctccaagac atcctcctgt ccaacgccca
    13381 aaccgccgcg gccatccgcc gggagctcga atcccggaag attctggccg tcaatctcct
    13441 cagctcgccc ggagccggga aaaccacgtt gctcgaggcg ctggccgagc ggctcaaggg
    13501 acgggcccgg atggcggtga tcgagggcga tatcgagacc gagcgcgacg ccgagcggat
    13561 ccgagcccgg ggaatcccgg cgtggcagat caccacgggc ggcgcctgcc atctcgaggc
    13621 gaagatgatc ggcaaggttc tgccggacat cccgaccgac atcgacttct tgttcatcga
    13681 aaacgtcggc aatctcgttt gcccggccgg ttacgatctt ggagaacggc tccgcatggt
    13741 tcttctttcc gcccccgagg gagacgacaa gccgaaaaaa tatcctctgg ccttcgtcac
    13801 ggccgatgtc ctcctcctct ccaagtccga ccttcgcccc ttccttcctt tcgacgcgga
    13861 gaaggcccag gccgaagcct tggccctcaa tccgaagctc cgggtgttca tcatctcggc
    13921 cgtcagcggc caggggctgg acgatcttgt cgactttctt ttccgcgccc gggaggagat
    13981 cgcctcccgt gcatgagctt tctctggtgg cctcgatgtt cgagaccctc ctcgaacaag
    14041 cccgccttca taaggcccgc cgcatcaccg gagtgaggat ccaggtcggg gccctggccg
    14101 gagtggttcc cgagcttctc gaaagcgcgt tcgactcgta taagaagggg acgatcgccg
    14161 acggagcggt tttgaccatc gtcaagccgc cgtttaccat ccgctgccgt gcctgcggcg
    14221 acgaaaccgt ccgggaagac ttcgccctgg cctgccccgc ctgcgcctcc accgacctcg
    14281 agatcgttca cgggatggag ctggtcctcg aaaaaatcga gatggagacg gacgacccgc
    14341 ttccggcgcc ttagacgacg atggcgccgg aggcccctcg gcggcttccg gccggataaa
    14401 acatcccgtt tcgcgagaga cgtcgatcag cgcagatacc caagcgatct cagcgtttcc
    14461 cgcgtttttt catcggccgt cgggccgggg ggttcgggtg aaaaatgcgg gatatgaagg
    14521 cccgcccgcc aatgctcgag aagacgctcg agccgcgccc cctggggcga ggaacgggga
    14581 tccaagggga tttcctctcc gggatccgcg gccaggttat aaagaaagga tcgtccgtcc
    14641 gagcgggaga ccagcttcca ggaatccgtc tgaaccgcct tctggctgag gctgaaggcg
    14701 gaggcatccg cgccgggcag ctcggcaagc gccccgagct cggagagggg aagttcgtat
    14761 tcagaaacgg cggcttcctc ctccggggcg attcccgccg tcgggccggc gtccggaaga
    14821 agcccgaaag cgctgggcca gttgtccgcc ggcgagacgc cggcgagctt caaaacagtc
    14881 gggaagatcg atcccgtcga cacgacgccc gcgtaccgct tgtttttccc gaaaaccctc
    14941 ggatacctca cgatcaaggg aacgcggaga agggtctggt ggatggagaa cgaatgattc
    15001 aacaagccgc cgtgctcgcc gaggttctcc ccgtgatcgg aggtaatgat cacgacggtc
    15061 tcctcgagca aacccctctc ccgaaacatc gcgatcaggc gctccaagca ggaatccagg
    15121 aaagcaagct cgccgtcgta cagctcggcc agccggccgt aatcggccgg cctcatcagg
    15181 gtcgggaacg cgatcagcct ccagacctcc ggatagcaag cgtcggtcat cagcttgcgg
    15241 acgggaggag aaggccgatt cttcaggaag cgtcggcgat acgccggagg cggattatag
    15301 ggaagatggg gcggcatgta gttgacgaaa atgaaaaacg gccggttctt gtcccgccgg
    15361 ttttgcgaat cgagccaccg ggcgatccaa ggctgcgtct cggcggccgc ccggtcggac
    15421 cgcttttttc ggggcgatcc caaggccgcg cccagcccct ttaggatttt caggttctcg
    15481 acgggatcca ggcctcttgc atcccacagc ccggaaagcc ggaacatttc atagaagcgg
    15541 tcgaatccct ggtcgaagtg aaacaaagag gacacattcg gattggagga aaaagcggcc
    15601 gtggaatagc cggcgctcct caagagttcg gccatggttt ccaattccgg acggagcgtg
    15661 cgatgctcga aggtcgcttg atgaacctcg gcgtactgtc cggtgaaaag ggaagcgtgg
    15721 gccggaaccg tccaggggct gggagaaacc gcccgctcat aaacggcgct ttcggccgcc
    15781 agccggcgga gaaacggcat cgtttcccgc tccccttccc ttaaggaaac atgatccgcc
    15841 ctggccgtat ccatcacgat gagcagaacg gacggggatc cggccggagg agcgggtcgg
    15901 cccgcggggg cgccgccgcc ggcatcaatt ttcccggcgc gggttcctcc tctgtccagg
    15961 gcgatgatta ttaggacgag accgaggccg gcaaaaccgg cgatccgtaa aaaacgcgtc
    16021 cgcctgccgg gaggttgtct ccaggtcgcg atcccggcgg ccgagagaac gaaggccgtg
    16081 gccgcgactc cggccgccag ccccgcgaaa tccttcgccc ctgacacgag gatggccgtc
    16141 cggccggcca ggaggcccgc cgccgaaacg aaaagaacgg tcaaaacaaa atcaaaaaga
    16201 gccttcttgc aaaaagaaat cacggtcgtt ttctgccggg cgggcgcgtc tccgccggcc
    16261 tcgggaaaaa cggcgccgac ggcggccgtc cccagtcctc cgatcattcc ccacagggaa
    16321 agggagataa ggaacgccgc caatgatgat ccggccaggc cgccgccgga ttgggcgacg
    16381 gcgaagagcg cgtcgacgag cccggccgcc aagccggccg cgacccccgt ccggagcccc
    16441 ccccaaactg ttcctttatt caccgctcct cccaagacga ggggaagccg aggacgcctc
    16501 aggcgcgcgg cggtttcctc aggaaatttc cccggtcaag taggctttca tccggggatc
    16561 cttcggctcg gcaaagacct cggccgccgg gccgtgctcg accagctccc ccaggtacat
    16621 gaaaacgata taatccgcga tccgccgggc ttggcggaga atatgggtca cgacgatgac
    16681 cgtataatcg gttttcaggt cgatcaaccg ctgctcgacg gtgcggctgg attgcgggtc
    16741 aagggcggag gtgggctcgt cgcccaggat gatctccggt tcgacggcca ggccgcgggc
    16801 caggcacagc cgctgctgct gaccgacgga cagccgggag gcgggctcat gaagacgggt
    16861 tttcacctcg tcccagagcc cggcggccgc aagctggcgc tcgacaatct gccgcagctt
    16921 cgctttctct cgaacgccgt ggatccgcgg accataggcg atgttgtcga aaatcgacat
    16981 cggcagaacc tgcggtttct gggacaacag ccccattttc ttccggatcc gcgtcacctc
    17041 gatcttcgga tcgtaaatgt tttccccgtc gaccaggacc tgaccctcga ccttcacgcc
    17101 atccactttg tccaaaagcc ggttgagggt tttaagcaag gtcgtcttgc cgcagcccga
    17161 ggggccgatg atggccgtga cccggtgatc agggagatcc accgagacgt ccttaaggac
    17221 tttctgccgg ccgaaggtca ccgtcaggtt cttgatggaa atatgcgtct tggcgttcat
    17281 ggcaatcctt cggtcaa
//
LOCUS       DAOOXH010000050        17155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_3233_length_17155_cov_7.709006, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000050 DAOOXH010000000
VERSION     DAOOXH010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 17155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17155
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3233_length_17155_cov_7.709006"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PKV64_07110"
     CDS             complement(<1..628)
                     /locus_tag="PKV64_07110"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPN16884.1"
                     /translation="MKKAAVFLSLSIFLASLFVFIADAQAGVQGSIKGVVKDKKGNVI
                     EGVKITIVSLQYSAVKYTLKTNAKGEYIQIGLQPDYYQVKAEKDGFFPIFVEKQVRIQ
                     DVLEVNLEMEEGNYYVGGESPGEKDFKQGNDLFQAGKYEEAIAAYEAAIGKEAGEPTY
                     LNNLGTTYMKIGNNEKAIATFTKMLEIQPESYSANKNLGSLFGEAKDYQ"
     gene            complement(843..1598)
                     /locus_tag="PKV64_07115"
     CDS             complement(843..1598)
                     /locus_tag="PKV64_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16885.1"
                     /translation="MEKEDFLRTAGIVGTVGVLGGVTKSVRITLGNERGTHDAHVQTI
                     DQAAAKFEGQGGQWELNFRDTYKFNVAAYLLDRMLDLNMVPVTVERIVDGKPSAVTWW
                     IDDVLMDEKERLAKNVTPPRWARPNWNRQINILLVFDQLIANRDRNLGNIIITKNWTV
                     WLIDHTRAFRMYKDLIDPGNLTACDRALLADLRRLDRPSLEENLGGCLNGMEIDGLLA
                     RRDRIVEIFDGKIAREGEDAVLFDYLIARRPRR"
     gene            complement(1716..1791)
                     /locus_tag="PKV64_07120"
     tRNA            complement(1716..1791)
                     /locus_tag="PKV64_07120"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1756..1758),aa:Ala,seq:ggc)
     gene            complement(1931..2389)
                     /locus_tag="PKV64_07125"
     CDS             complement(1931..2389)
                     /locus_tag="PKV64_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16886.1"
                     /translation="MKCLDFLDDAAALEDALAESYGLLAGLTADPGVSEKLNRLRREE
                     ENHARIIRTGKNYVRRAPDLFGEAIISGAELQAGLASCREVTADIRSARLPFREGLVR
                     LAGLEDQFEKIHLDSALEVKDENLGRLFRRMARDDREHRVVLEEILQRPV"
     gene            2538..4409
                     /gene="ftsH"
                     /locus_tag="PKV64_07130"
     CDS             2538..4409
                     /gene="ftsH"
                     /locus_tag="PKV64_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPN16887.1"
                     /translation="MAVKRNQSLKNMLFWLVAGILVITLWSVFQAREMSSADIPFSRF
                     IDDVEAQRVEKVTIDGVNVTGRYRSDGAAFKTIIPSQYDGLVPILRQNKVDITVKDAN
                     RATWLSVLFTWSPLLLIVVFWFFFMRQMQAGGNKAMSFGKSRAKLFSTVQKKVTFKDV
                     AGVEEAKEELKEIVGFLKEPQKYQKLGGRIPKGVLLVGAPGTGKTLLARAIAGEAEVP
                     FFSISGSDFVEMFVGVGASRVRDLFENGKKHAPCLIFIDEIDAVGRQRGAGLGGGHDE
                     REQTLNQLLVEMDGFDSNEGIILIAATNRPDILDSALLRPGRFDRRIVVNMPDVKGRE
                     EILKVHTRKIPLADDADLKVIARSTPGFSGADLANMINEAALNGARLEQDKVRMKDLE
                     YAKDKVLMGVERKSMIISDEEKKSTAYHEAGHALVAAMIPEADPIHKVTIIPRGMALG
                     LTQQLPLDDRYTYGKAYLESQLAVLMAGRVTELMFLNQITTGAANDFERATEIARRMV
                     CQWGMSDLGPLTFGERDDLIFLGRELTAHKNYSEKTAEAIDEEVKKIVKRNYGRAEKI
                     IGEHRDLLEAIVKQLLEREVLSSDEINAILGKPASSPPPSSEEEGKSPEPAGNREVK"
     gene            4406..5299
                     /gene="folP"
                     /locus_tag="PKV64_07135"
     CDS             4406..5299
                     /gene="folP"
                     /locus_tag="PKV64_07135"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPN16888.1"
                     /translation="MRTGTVFRVKGARHLLGTRTWVMGILNVTPDSFFDGGRYFDPGP
                     AVDHGLRLADEGADIIDVGGESARPGSDPIPAEEEIRRIVPVISSLRTQTRVLLSVDT
                     TKFETARAALGAGADIINDISSFEMDPRLLVLAAETGAGFILMHMQGVPKTMQAAPRY
                     EDVVAEVRGFLAEKIEIAAAYGLNKESLAVDPGIGFGKRLEDNCALIRELETIAGIGR
                     PVVMGVSRKSMIGKILDVPPEERLEGSLAAAVVGLARGAHILRVHDVQSTRRAARVAD
                     AILSRPAGGENPEKREAGRAR"
     gene            5289..6059
                     /gene="cdaA"
                     /locus_tag="PKV64_07140"
     CDS             5289..6059
                     /gene="cdaA"
                     /locus_tag="PKV64_07140"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HPN16889.1"
                     /translation="MLDNILTALHLFKLVDFIDILVVSVIIYSFFILIKESKAYPMAA
                     GLGLIGLLYLVTRWANLFVANWLIRNFVAYIIITVIILFQTEIRRFLTRIGSRSFRKP
                     LALRSFQEKIEDLCLAVDYMADRKIGALIAVENEVSLEPFAERGTPIDAVLTKDLLVS
                     LFFPHSPLHDGAVIIRGDKIMAAGCLLPLPATHEMRVSFPTRTRHLAAIGLTQETDAA
                     VVIVSEESGGISLATGGTLERLIDRNKLEDRLLEYLKK"
     gene            6059..6721
                     /locus_tag="PKV64_07145"
     CDS             6059..6721
                     /locus_tag="PKV64_07145"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HPN16890.1"
                     /translation="MKKIRDLFLRNGLLKLLSFLMAVFLWIALMPEEKISSEKTFQVR
                     LDAQGLPAEFEMTERPPSMIDVTIRATNRMLGQMTSTDIQAVLNLNRANVNQEDYPLN
                     PDIVTVPAGAKAVRVFPNKVHLKIERSAEVLMEIQPVLVGQPKAGLKIDQVDLLPSKV
                     FVRGPESKIKPQDRIRTSPMDITGLDQAKTFEVDLILPRPELRFATAQTRASVRITLI
                     EK"
     gene            6752..8092
                     /gene="glmM"
                     /locus_tag="PKV64_07150"
     CDS             6752..8092
                     /gene="glmM"
                     /locus_tag="PKV64_07150"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPN16891.1"
                     /translation="MQKLFGTDGIRAAAGEYPLDEPSVRTLGRALVDLLQAEGLPPRI
                     LIGRDTRESGDWIEEALIRGISSAGGQCASAGIIPTSAVSHLARTFAFSAGIVVSASH
                     NPYKDNGIKIFSSAGLKISDAWEDRLETEIARRPEVRGLEKANVRPDPAYRREYEHFL
                     RRRFAPGPGPKGMKIVLDCANGSASAIAPEVFGGLGFTIVATEAEPDGRNINEECGAL
                     HPEKLAQAVLARKADLGIAFDGDADRALWVDHRGRILSGDHTLFVLSRAMKARGLLRG
                     RSVVATTMSNMGLEKALAADGLSLVRTRVGDKYVLEKMLSIRANLGGERSGHTILSDE
                     CPTGDGLLTALKLLEVMVEKNLPLADLTRDYAEYPQVLKNVRVARKPAMDGLPELVAA
                     IADVRERLGKNGRLEVRYSGTEPLARVMVEGEDEDLIEILAARITAAIRKTIGV"
     gene            8181..8906
                     /locus_tag="PKV64_07155"
     CDS             8181..8906
                     /locus_tag="PKV64_07155"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HPN16892.1"
                     /translation="MKLSFNVDHFASLRQARRAAEPEPVLAALIAEQAGAAGITAHLR
                     GDRRHINERDLRMLREAIKTKLTVEMAAAAEMKAAALAVRPDIVCLVPEKPEELTTTG
                     GLDVVGRRKALAAHITALTKGGIKVSIFVDPSPAQIRAIADLGVRKIEIHTGVYAEAA
                     PGQAREKALDEIRAAASAAARLGLDVTGGHGLDYHNVGPIAAIPEMTELSIGFSIVAR
                     AAIVGVDRAVRDMVALLNPRRAR"
     gene            8967..10205
                     /locus_tag="PKV64_07160"
     CDS             8967..10205
                     /locus_tag="PKV64_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HPN16893.1"
                     /translation="MIIRVNTSRLQSNLEALGRIGRDAAGGITRPSFSLADLEARAWF
                     RARIEEGGLEYREDGAGNQFGRLEGAAGPEANVVMAGSHLDTVPNGGMFDGAAGVVAA
                     LECLRTIREEGLRLRLPLEAASFTDEEGNLVGDFLGSRAFTGKLDRGLLERGTTSFGR
                     PLAEILSATPYTIETILSAADRAPAVAAYLELHIEQGPVLETEGISLGLVETINGKHY
                     RWCAFEGESGHSGTVPLELRRDALLGLADFALRATRHVASARYGSFVTIGKALIQPGT
                     FSSIPRRADFSLEFRSSSKESMEALERELLGIAGEVASARRLRFASRLMDRTDPYRIP
                     GRLAGILAAEADRLGYAHMPVTSGAGHDAQILGTVTDAAMIFIPSPDGLSHAPEESLR
                     WEDLEKGANLLLAALLRLAS"
     gene            complement(10210..10581)
                     /locus_tag="PKV64_07165"
     CDS             complement(10210..10581)
                     /locus_tag="PKV64_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HPN16894.1"
                     /translation="MERLGLRDSDFVERFMRSGGKGGQNVNKVETCVHLIHVPTGLSV
                     KCQRERSQAINRFLARRILVDKIETLRKGEASAREQRIEKIRRQKRKRSRRAKAKMLD
                     DKRRQGEKKAGRSFRPDPDVF"
     gene            complement(10631..11458)
                     /locus_tag="PKV64_07170"
     CDS             complement(10631..11458)
                     /locus_tag="PKV64_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPN16895.1"
                     /translation="MDKRDVLGISHFSDYFKISQLVFELKALEKVAAVEELIDVLAKQ
                     KLVRNRKMILTRLMDRETLESTALGDGIAVPHARVDSDGEIVIAVGRSSEGIDFSAPD
                     KKKVHLIILIIWNPTLPGLFNHLFAGLAHFLRDPHFRRRIFEAKDKNALWEALGEIEL
                     RLPQEDKIISRAGLLRKLQEIEKKKKKATKAQMPTLKAQADLLREELDEALLTRFQKL
                     MERYGFAVAEVEDGVCQGCNISVSTEMSSAIEGSNDIYICENCGKYLVAASPKKKKK"
     gene            complement(11502..13133)
                     /locus_tag="PKV64_07175"
     CDS             complement(11502..13133)
                     /locus_tag="PKV64_07175"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPN16896.1"
                     /translation="MTRAKTAFFLLAAAALAAALPACRTDASAPRPITPAEIEAHIRF
                     LSDDLLEGRALGGRGIEIAALYQEQVFRGLGLEPAFPGGSYRQTFEARECAPDPKPSL
                     SVAAGGVNVSLEPREEFVVNSYREDAPPSVSGELVYAGYGIQAPERDWDDLKGMDLAG
                     KVLLVEINEPGNVPGGLFDGESMTYYGRWVYKYEKAAELGAAGCLIIHDAKGAAYGWD
                     VVRNGWSNEEYFLPDKPQRLFFRGWISAGAAERICAAAGRDRGALRAAAETREFVPVA
                     LGATAEVRQRCRYRSVSASNVAGLLRGSVRGPGERFIVFSAHYDHLGVDPTRDGDRIF
                     NGAVDNGSATAAMLALARLCAEAPERLRATLVFAAVTGEESQFLGSDYFVRHLPFPAD
                     AVLADLNFEMTGVWGETEDVYAIGASHSDLDEIASRAAARLDLRYTPERDTELGYFFR
                     SDQFSFARNGLPAAWLHEGVVSRTRGPEYLPEKTAAYKKNGYHQVGDEIQPDWDFAGT
                     IQIALWAREIAALLDASPGFPQFKPESSFKRKQPH"
     gene            complement(13135..14109)
                     /locus_tag="PKV64_07180"
     CDS             complement(13135..14109)
                     /locus_tag="PKV64_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPN16897.1"
                     /translation="MTCCGLIGTITADTIEIEDGRTLRAFRSVGGILYQAAALSGLGV
                     PAALFSRCGGELRPEVEAVLGGRPDLDRRGLRFVPGPGNRVRLRYAGRSREREEILES
                     VVPPLDPGPVLEALPGLDFLMMVFNSGFDIELADWRAVAGRASCPVWMDIHSLALEPR
                     VGSRRGYRTVPEWEAWVEGAAYLQANRREAACLVGRPDREAGEAEIEELARRAFRLGV
                     RAVIVTLGRAGAAVSTPESVRRIPPAAAPVFVDPTGCGDVFAAAASAALLRGGTVFEA
                     AEAGAALAARAAGVSGVHEIFGLASRERESRRGPDRPPPSSAGGKGEK"
     gene            complement(14320..15564)
                     /locus_tag="PKV64_07185"
     CDS             complement(14320..15564)
                     /locus_tag="PKV64_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HPN16898.1"
                     /translation="MQDTELYRHVLGIEDPWRVDRVQLSVREEQVDVWAVHGESIRFS
                     CPECGTPLPIYDHAPERVWRHLDTCQFKTFLHARIPRVECPAHGVKQVRVNWAEEKSR
                     FTALFERLAIMVLQETNIEGAGRILRISWDEAWHILERAVRRGQERKKPGIVKKMGVD
                     EKSLGKGHNYLTLIYDLQRSTVEHIEDDRKKESLDGYFRKMTPAQRAKIKAIATDIWD
                     PYLASIREHVPGYEEKLVFDRYHLMTHMVQAVDMVRKQEHRDLRGEGNEILSGTKYLW
                     LYSRENLPVKKRKAFSALRRKRLRTARAWAIKEDLRRFWSYQTETWARKHFSGWYGWA
                     IRSQLKPVKAVARMFRKYLPNIMTYLRHRITNAVSEGVNSMIQTIKKMACGFRNREHF
                     KLAIFFHCGGLNLYPVTHKNVG"
     gene            complement(15903..16898)
                     /locus_tag="PKV64_07190"
     CDS             complement(15903..16898)
                     /locus_tag="PKV64_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPN16899.1"
                     /translation="MNYRYLGRTGVRVSSLCFGTMTFGKEADEAVSGELFRACREAGI
                     NFFDCADMYQAGRAEEILGRLIEDRREEVVVTTKGFFPTGTNPNAQGASRYHLRNAVE
                     ASLRRLRTDRIDVYFIHRFDDRTPLEETLRALDDLVRQGKILYPGLSNFAAWQAEKAL
                     GLSALHGWAPPGVLQPMYNLLKRQAEAEILPMAEAEGLGVIPYSPLAGGFLSGKFGSN
                     RRPASGRLTEHKIYQARYFEEANFEIVDRFCEFARARGWSPAALAAAWAGGHPAVTAP
                     IIGARNLAQLRDVLGSLEIPMDDGLRAEISALSPAPPPATDRSEERTDTHFFIRT"
BASE COUNT         3174 a         5614 c         5257 g         3110 t
ORIGIN      
        1 cctgatagtc cttggcctcg ccgaagagcg aacctagatt tttgttggcg ctgtagcttt
       61 ccggctggat ttccagcatt ttcgtgaagg tcgcgatggc tttctcgttg ttcccgatct
      121 tcatgtaagt cgttccgagg ttgttgagat aggtgggctc gccggcttcc ttgccgatgg
      181 cggcctcgta agcggcgatc gcttcctcgt acttgccggc ctggaacaga tcgttcccct
      241 gcttgaaatc tttctcgccg ggcgactccc ccccgacgta ataattgcct tcctccatct
      301 cgagattaac ctcgagcacg tcctggatcc tcacctgctt ttcgacgaag atcgggaaaa
      361 agccgtcttt ctccgccttg acctgatagt aatccggctg aaggccgatc tggatgtatt
      421 cgcccttggc gttggtcttg agcgtgtatt tcacggccga atactggagg gagacgatcg
      481 tgatcttgac gccctcgatg acattccctt ttttatcctt gacgacgccc ttgatcgacc
      541 cttgtacgcc ggcctgggca tcggcgatga aaacaaaaag agaagccagg aaaatcgaaa
      601 gactgaggaa aacggcggct tttttcatgc tcgcatcctt tctcaattca ggcggtctca
      661 acctctccta tcctaccgtc ccggcgcggc caagtcaaga atacgggggg gcggccgcgc
      721 gctccaaaat ccattgtgca agattcatac caaggatgcg gacgcccgat catcagggtg
      781 atcgcggtcg gcggtccgtc caaaaacggc gacagccgtc tccggcggag gccggaacgc
      841 cctcagcgcc gcggccggcg ggcgatcaag taatcaaaca ggacggcgtc ctccccctcg
      901 cgggcgatct tgccgtcgaa aatctccacg atccggtccc ggcgggccag gagcccgtcg
      961 atctccatgc cgttgaggca gccgcccaga ttctcctcga gggacggccg gtccagccgg
     1021 cggaggtcgg ccaaaagggc ccggtcgcag gccgtgagat tcccgggatc gatcaaatcc
     1081 ttgtacatgc ggaatgcccg ggtgtggtcg atcagccaaa ccgtccaatt cttcgtgatg
     1141 atgatatttc ccaggttgcg gtcccggttg gcgatgagct gatcgaagac cagcaggatg
     1201 ttgatctgcc ggttccaatt gggacgggcc caccggggcg gagtcacgtt cttggccagg
     1261 cgctcctttt catccatcag gacgtcgtcg atccaccagg tgacggcgga cggtttcccg
     1321 tccacgatcc tttcgaccgt caccggaacc atgttcaggt cgagcatccg atcgaggagg
     1381 taggcggcga cgttgaattt ataggtatcc cggaaattga gctcccattg gccgccctgg
     1441 ccctcgaact tcgccgccgc ctggtcgatc gtctggacgt gggcgtcgtg cgtcccccgc
     1501 tcgttcccca acgtaatccg gacggacttg gtgacgcccc ccaggacgcc gaccgtgccg
     1561 acgatcccgg ccgtccgcag aaaatcctct ttttccaaac ggctccacga ggccgggccg
     1621 gccgacgcgt cttgacggcc gacgcccgga gggagagccg cctcagcggc ggaaacgaag
     1681 ggaaaagcaa tcaagaacag gaaaaggagc ggcgctggtg gagctgacca ggatcgaact
     1741 ggcgacctct tgaatgccat tcaagcgctc tcccaactga gctacagccc cacgggaagg
     1801 gcccagatca tacgccaaaa ggcggcgatc gtcaagcggc cttcccgctt ccggaagctc
     1861 tttcttccgc cgtttccggc cggattcgag gcccgcggtt ccgcgccgcg gccgccgcgt
     1921 cgattccttc tcaaaccggc cgctgaagga tttcctccag gacgactcga tgctcccggt
     1981 cgtcccgggc catccgccgg aagagccggc cgaggttttc gtccttcacc tccaaagcgg
     2041 agtccagatg gatcttttca aattgatcct ccaggccggc caggcggaca agcccctccc
     2101 ggaacgggag ccgggccgag cggatatcgg ccgtcacctc ccggcaggaa gccagccccg
     2161 cctgaagttc cgccccgctg atgatcgctt ccccaaaaag gtcaggggcc cggcgcacat
     2221 aattcttccc cgtccggatg atccgggcat ggttttcctc ttcccggcgg agcctgttca
     2281 gcttttccga cacgccggga tcggcggtca ggccggcaag caagccgtag ctttcggcca
     2341 aggcgtcctc cagggcggcc gcgtcgtcca agaaatcaag gcacttcatc ccgttctcct
     2401 tttcggtcgg atggttgcga agttgattct tattatagat gaaaaccatc cgggccgccc
     2461 gcaagaacgc cgcgacgcaa ttcctccttt ccccttccct gattccgtgt taaaatgagg
     2521 cgcccaaagg aaggtccatg gccgtcaaac gtaatcaatc tctgaagaat atgctgtttt
     2581 ggctggtggc ggggattctc gtcatcaccc tctggagcgt tttccaagcg cgggagatgt
     2641 ccagcgcgga catcccgttc agccggttca tcgatgacgt cgaagcccag cgcgtcgaga
     2701 aagtcacgat cgacggcgtc aacgtcaccg ggcgctaccg gagcgatgga gccgcgttca
     2761 agacgatcat cccctcgcaa tacgacggcc tcgttcccat cctccgccag aacaaggtcg
     2821 acatcaccgt caaggacgcc aaccgggcga cctggctgtc ggtcctgttc acctggtcgc
     2881 cgctcctgct gatcgtcgtc ttctggttct ttttcatgcg gcagatgcag gccggcggca
     2941 acaaggccat gtccttcggc aaaagccggg ccaagctctt ctcgaccgtc cagaaaaaag
     3001 tgacgttcaa ggacgtggcc ggcgtcgagg aggccaagga ggagctcaag gaaatcgtcg
     3061 gtttcctcaa ggagccgcag aaataccaga agctcggcgg gcggatcccc aagggggttc
     3121 tcctcgtcgg ggccccgggc acgggcaaga cgctcctggc ccgggcgatc gccggcgaag
     3181 ccgaggtccc cttcttttcc atcagcgggt ccgatttcgt cgagatgttc gtcggcgtcg
     3241 gggcctcgcg ggtccgggac ctgttcgaaa acggcaagaa gcacgccccc tgtctcatct
     3301 tcatcgacga gatcgacgcc gtcggccgcc agcgcggggc cgggctgggc ggcgggcacg
     3361 acgagcgcga acagacgctg aaccagctgc tcgtcgaaat ggacggcttc gactccaacg
     3421 agggcatcat cctcatcgcc gcgaccaacc gtcccgacat cctcgacagc gcccttctcc
     3481 ggccgggccg cttcgaccgg cggatcgtcg tcaacatgcc cgacgtcaag ggccgcgagg
     3541 aaatcctcaa ggtccatacc cgcaaaatcc ccctggccga cgacgccgac ctcaaggtca
     3601 tcgcccgctc gacccccggt ttcagcgggg ccgacctggc caatatgatc aacgaggccg
     3661 ccctcaacgg ggcccgcctg gagcaggaca aggtcaggat gaaggatctt gaatacgcca
     3721 aggacaaagt cctcatgggc gtcgagcgga aaagcatgat catcagcgac gaagagaaaa
     3781 agagcacggc ctatcacgaa gccgggcacg ctctggtcgc ggccatgatc cccgaggccg
     3841 acccgatcca caaggtgacc atcatccccc gggggatggc cctcggtttg acccagcagc
     3901 tccccctcga cgaccgctac acgtacggga aagcctatct cgaatcccag ctggccgtgc
     3961 tgatggccgg ccgggtcacg gagctcatgt tcctcaacca aatcacgacc ggcgcggcca
     4021 atgatttcga gcgggccacg gaaatcgccc ggcggatggt ctgccagtgg ggcatgagcg
     4081 acctcggccc gctgaccttc ggcgagcggg acgacctgat tttcctcggc cgcgagctga
     4141 ccgcccacaa gaactacagc gagaaaaccg ccgaggcgat cgacgaggaa gtcaagaaga
     4201 tcgtcaagcg caattacggc cgggccgaga agatcatcgg cgaacaccgc gatctgctgg
     4261 aagccatcgt caagcagctc ctcgagcggg aagtcctgtc gtcggacgag atcaacgcga
     4321 ttttgggcaa accggcctcc tcccctcccc cctcatccga agaggaggga aaatcgccgg
     4381 aaccggccgg gaaccgcgag gtgaagtgag gaccggaacc gtcttccgcg tcaagggcgc
     4441 gcgtcacctt ctggggacgc ggacctgggt gatggggatc ctcaacgtca cgccggattc
     4501 ctttttcgac ggcgggcgct atttcgatcc cggaccggcc gtcgaccacg gcctccggct
     4561 ggccgacgag ggcgccgaca tcatcgacgt cgggggggaa agcgcccgcc ccggctccga
     4621 ccccattccg gccgaggagg agatccggcg catcgttccg gtgatttcct cgctgcgaac
     4681 ccaaacgcgg gttctcctct cggtcgacac gaccaagttc gagacggccc gggcagcctt
     4741 gggcgccggg gcggacatca tcaacgacat cagctccttt gagatggacc cccgtctgct
     4801 cgtcctggct gcggaaaccg gcgcgggatt catcctgatg cacatgcaag gcgtcccgaa
     4861 aaccatgcag gccgccccgc gctacgaaga cgtcgtggcc gaggtccggg gctttctggc
     4921 cgaaaagatt gaaatcgcgg ccgcctacgg cctgaacaag gagagcctgg ccgtcgatcc
     4981 gggcatcgga ttcggcaaac ggctcgagga caattgcgcc ttgatccggg aattggaaac
     5041 gatcgccggg atcggccggc cggtggtgat gggcgtgtcc cggaagtcga tgatcgggaa
     5101 gattctggac gttcctccgg aagagcgcct ggagggatcg ctggccgcgg ccgtcgtcgg
     5161 cctggcccgc ggggcgcaca tcctccgggt tcacgacgtc caatccaccc ggcgggcggc
     5221 ccgcgtggcc gacgccatcc tctcccgccc ggccggcggg gaaaatcccg agaagagaga
     5281 ggccggccgt gctcgataac atcctgaccg cccttcatct cttcaagctg gtcgatttca
     5341 tcgacatcct cgtcgtctcg gtcatcatct actccttttt catcctgatc aaggaatcca
     5401 aagcctatcc catggccgcc gggctcgggt tgatcggcct gctttacctc gtcacccgct
     5461 gggccaacct gttcgtcgcc aactggctca tccgcaactt cgtcgcctat atcatcatca
     5521 ccgtcatcat cctcttccag accgagatcc ggagattcct gacgcgcatc ggctcgcggt
     5581 cgttccgcaa gcccctggct ctccgctcgt tccaggaaaa aatcgaggac ctctgcctgg
     5641 ccgtcgatta catggccgac cgcaagatcg gggccttgat cgcggtggag aacgaggtca
     5701 gcctcgagcc cttcgccgag cggggcacgc ccatcgacgc cgtgctgacc aaggatctcc
     5761 tggtcagctt gtttttcccg cattcgcccc tgcacgacgg ggcggtgatc atccggggag
     5821 acaagatcat ggccgccggt tgccttctcc cgctcccggc cacccatgaa atgcgggttt
     5881 cctttccgac ccgaacccgt cacctggcgg ccatcggcct gacccaggaa accgacgcgg
     5941 ccgtcgttat cgtctcggag gaatcgggcg gaatctcgct ggccacgggg ggaaccctcg
     6001 agcgcctcat cgaccggaac aagctggaag accggctcct ggaatatttg aaaaaatgat
     6061 gaaaaagatc agggatcttt tcctccgcaa cggacttctc aagctcctgt cgttcctcat
     6121 ggccgttttc ctctggatcg ctctgatgcc ggaggaaaaa atttcttccg aaaagacgtt
     6181 ccaggtccgg ctcgacgccc agggcctccc ggccgagttc gagatgaccg agcggccgcc
     6241 gtcgatgatc gacgtgacca tccgggcgac gaaccggatg ctcggacaga tgacctcgac
     6301 ggacatccaa gccgttctca acctgaaccg ggcgaacgtc aaccaggaag attacccgct
     6361 gaaccccgac atcgtgaccg tcccggccgg ggccaaggcc gtccgggttt ttcccaataa
     6421 agtccatctc aagatcgagc ggagcgcgga agtcctgatg gagatccagc cggtcctcgt
     6481 cggccagccc aaggccgggc tcaaaatcga ccaggtcgat ctcctcccgt ccaaggtctt
     6541 cgtccggggt ccggaaagca agatcaagcc ccaggaccgg atccggacca gtcccatgga
     6601 catcaccgga ttggaccagg cgaaaacctt tgaagtcgac ttgatcctcc cccggcccga
     6661 gctgcggttc gccacggcgc agacgcgggc ctcggtccgg atcactctca tcgaaaaata
     6721 gtcgggcggc gcgggaaggg ggaaagaaga aatgcaaaag ctgtttggga ccgacggcat
     6781 ccgggccgcg gccggagaat atcccctgga tgagccttcg gtccggacgc tgggccgcgc
     6841 cctggtcgac ctcctccaag cggaaggcct gccgccccgg atcctgatcg gacgggacac
     6901 gcgggaatcc ggcgattgga tcgaggaagc cctgatccgg ggcatctcct cggccggggg
     6961 gcagtgcgcc tccgccggaa tcattccgac gtcggctgtt tcccatctgg cccgcacgtt
     7021 cgcattctcg gccgggatcg tcgtttccgc ctcccacaat ccctacaagg acaacgggat
     7081 caagattttt tcgagcgccg gcctgaagat ctccgacgcc tgggaagacc gcttggaaac
     7141 tgaaatcgcc cgccggccgg aagtccgggg cttggaaaaa gcgaacgtcc ggccggaccc
     7201 ggcctatcgc cgggaatacg agcacttcct ccgccgccgg ttcgccccgg ggcccggccc
     7261 caaggggatg aaaatcgtct tggattgcgc caacggctcg gcctcggcca tcgccccgga
     7321 ggtgttcggc gggctcggct tcacgatcgt cgcgaccgag gccgagccgg acggccggaa
     7381 tatcaacgaa gaatgcgggg cgctccaccc cgaaaaactc gcccaggcgg tgctggcccg
     7441 gaaggccgac ttggggatcg ccttcgacgg cgacgccgac cgggccctct gggtcgatca
     7501 tcgcggccgg atcctcagcg gcgatcacac gctgttcgtc ctcagccggg cgatgaaggc
     7561 ccgcggcctc ctccgcggac ggtcggtcgt ggccacgacc atgagcaaca tgggcctgga
     7621 gaaagccctg gccgccgacg ggctctccct ggtccgaacg cgggtcggag acaaatatgt
     7681 cctggaaaaa atgctctcga tccgggccaa cttgggcgga gagcgctcgg gccacaccat
     7741 cctttcggac gagtgcccca ccggcgacgg cctgctgacg gccctcaagc tgctcgaggt
     7801 catggtcgaa aaaaatctcc ccctggccga tctgacccgg gactacgcgg aatatcccca
     7861 ggtcctgaaa aacgtccggg tggctcgaaa gccggccatg gacggccttc ccgagctcgt
     7921 cgcggcgatc gccgacgtcc gggagcgctt ggggaagaac gggcggctgg aggtccggta
     7981 ttccgggacc gaaccgctgg cccgggtcat ggtcgagggc gaggacgagg acctgatcga
     8041 gatcctggcc gcccggatca cggccgcgat ccgcaagacc atcggggtct gaagccgcgg
     8101 ccggccctgc gccggacgat ccctttgagg tataatcgga aagcgccgga aagagggatc
     8161 ctttccggga ggagtgcctc atgaaactgt ccttcaacgt cgatcatttc gccagcctgc
     8221 gccaagcccg gcgggcggcc gagcccgagc cggtcctggc cgccttgatc gccgagcagg
     8281 ccggcgcggc cgggatcacc gcccatctcc gcggcgaccg gcgccatatc aacgagcgcg
     8341 acctccggat gctgcgggag gcgatcaaga ccaagctcac cgtcgagatg gccgccgccg
     8401 ccgagatgaa agccgccgcc ctcgccgtcc gccccgacat cgtctgcctc gttccggaaa
     8461 aacccgagga gctgacgacc accggagggc tggacgtcgt cggccgccga aaagcgctcg
     8521 ccgcccacat cacagccctg accaagggcg gaatcaaggt ctcgatcttc gtcgatccat
     8581 cccccgccca aatccgggcg atcgccgacc tcggcgtccg gaaaatcgag atccataccg
     8641 gcgtttacgc cgaggccgcg cccggacaag cccgggagaa agccctcgac gaaatccgcg
     8701 cggccgcgtc cgcggccgcc cggctcggcc tggacgtcac cggcggccat gggctcgatt
     8761 atcataatgt cgggccgatc gcggccattc ccgaaatgac cgagctgtcc atcggatttt
     8821 cgatcgtcgc ccgggcggcg atcgtcggcg tcgaccgggc cgtccgcgac atggtcgctt
     8881 tgttgaatcc ccggcgcgcc cggtaggtcc gccgccctcg acggagaacg gcgcccggcc
     8941 ccggaaggcg cgggaggagg cccgccatga tcatccgcgt caacacgtcc cgcctgcaat
     9001 cgaatctcga ggctctcggc cggatcggcc gcgacgcggc cggagggatc acccgtccgt
     9061 ccttcagttt ggccgatctc gaagcccggg cctggtttcg agcccggatc gaggaaggcg
     9121 ggctggagta ccgggaggac ggagccggaa accagttcgg ccggctcgag ggggcggccg
     9181 ggccggaggc caacgtcgtg atggccggct cccatctcga caccgtcccc aacggcggca
     9241 tgttcgacgg ggcggccggc gtcgtcgccg ccctggaatg cctccggacg atccgggaag
     9301 aaggcctgcg cctccgcctg cctctcgagg cggcctcgtt caccgacgag gagggcaacc
     9361 tggtcggcga tttcctcggc agccgggcgt tcaccggaaa gctggaccgg gggctcctcg
     9421 agcgcgggac gacttccttc ggccggcccc tggccgaaat tctgtcggcc acgccgtaca
     9481 ccatcgaaac gatcctctcg gcggcggacc gggcgccggc cgtcgcggcg tatctcgaac
     9541 tccatatcga acaagggccg gtcttggaaa cggaggggat ctcgctgggt ctcgtcgaaa
     9601 ccatcaacgg aaagcattac cgatggtgcg ccttcgaggg ggaatcgggg cactccggga
     9661 ccgttcccct ggaattgcgg cgcgacgccc ttctcggatt ggccgacttc gccttgaggg
     9721 cgacgcggca tgtcgcctcg gcccgctatg gaagcttcgt caccatcgga aaagccctca
     9781 tccagcccgg caccttcagc tccatccccc gccgggcgga tttttccttg gaattccgaa
     9841 gttcctcaaa ggaatccatg gaagccttgg aacgggagct gctcgggatc gccggcgagg
     9901 tggcctcggc ccgccgcttg agattcgctt cgcgcttgat ggaccggacg gatccgtacc
     9961 gcatccccgg ccgcttggcc ggaatcctgg cggccgaagc cgaccggctc ggctacgccc
    10021 acatgccggt cacaagcggc gccggccacg acgcccagat tctcggaacg gtgacggacg
    10081 cggccatgat tttcatcccg agccccgacg gcctcagcca cgcgccggaa gaatccctcc
    10141 gctgggagga tctggaaaag ggggcgaacc tcctgctggc cgctctcctg cggctggcct
    10201 cttaacaggt taaaacacgt cgggatcggg acggaaggac cggccggctt tcttttcccc
    10261 ctgccggcgc ttgtcgtcca gcattttcgc cttggcccgc cgggaccgct tgcgcttctg
    10321 ccggcggatc ttttcgatcc gctgttcccg ggcgctggct tcgcctttcc gcaatgtttc
    10381 gattttatcg accaggatgc gccgggcaag aaaccggttg atcgcttggg agcgctcgcg
    10441 ctggcatttg accgaaaggc cggtcgggac atggatcaaa tgaacgcagg tctcgacctt
    10501 gttgacgttc tggccgccct ttccccccga tcgcatgaag cgctcgacga aatcggagtc
    10561 gcgcagtccg agccgctcca tcttttcccg gagggcggcg atcttttccg gacggacggg
    10621 tccgcacacc ctactttttt ttcttcttcg gcgaggcggc gacgaggtac ttgccgcagt
    10681 tttcacaaat gtagatgtcg ttgctgccct cgatggccga gctcatctcc gtggacacgc
    10741 tgatgttgca gccctggcag acgccgtcct cgacctcggc tacggcgaag ccgtaccgct
    10801 ccatcagctt ctggaaacgg gtcaagaggg cttcgtcgag ctcctcccgc agaagatcgg
    10861 cttgggcttt gagggtgggc atctgggcct tcgtcgcttt ctttttcttc ttctcgatct
    10921 cctggagctt gcgcaggagg ccggcccggc tgatgatctt gtcttcctgg ggaaggcgca
    10981 gctcgatctc gcccagggct tcccacaagg cgttcttgtc cttggcctcg aagatccgcc
    11041 ggcggaaatg cggatcccgg aggaaatgag ccagcccggc gaagaggtgg ttgaagagcc
    11101 ccggcagggt tggattccag atgatgagga tgatcaggtg gactttcttc ttatccgggg
    11161 cggagaaatc gatcccttcc gacgaccggc cgacggcgat gacgatctcg ccgtcggaat
    11221 cgacccgggc gtggggaacg gcgatgccgt cgcccaaggc ggtcgactcg agggtttccc
    11281 ggtccatcag ccgggtcagg atcattttcc ggttccggac gagcttttgc ttcgccagga
    11341 cgtcgatcaa ttcctcgacg gccgcgactt tctccaaagc tttgagctcg aagacaagct
    11401 ggctgatctt gaagtaatcc gagaagtgac tgatgcccag gacgtctctt ttatccatgc
    11461 gccaccgtgg ctgatatatt accctaggca gccgtccgcg gtcaatgcgg ctgttttctt
    11521 ttgaacgagc tctccggctt gaactgcgga aagcccggcg aggcgtcgag aagggcggcg
    11581 atttcccggg cccagagagc gatctggatc gtcccggcga aatcccagtc gggctggatt
    11641 tcgtcgccga cttgatggta gccgtttttt ttgtaagcgg ccgttttctc cggcaggtat
    11701 tccggccccc gcgtccggga gaccacgccc tcgtgaagcc aggcggccgg aaggccgttg
    11761 cgggcgaagc tgaattgatc ggaccggaaa aaatagccca gctccgtgtc ccgctccggc
    11821 gtgtaccgga gatcgagacg ggcggccgcc cgggaagcga tttcgtccaa gtcggagtgg
    11881 gacgccccga tcgcgtagac atcctcggtt tcgccccaga cgccggtcat ctcgaaattg
    11941 agatcggcca gaaccgcgtc ggctggaaag ggaagatggc ggacgaaata atccgagccc
    12001 aggaattggc tctcctcgcc ggtcacggcg gcgaaaacga gcgtcgcccg cagccgctcc
    12061 ggcgcctcgg cgcagagccg ggccagggcc agcatcgccg ccgtggccga gccgttgtcc
    12121 acggccccgt tgaagatccg gtcgccgtcg cgggtcggat cgacgcccaa gtggtcatag
    12181 tgggcggaaa agacgatgaa ccgctccccg gggccccgga cggatccgcg gagcagcccg
    12241 gcgacgttgg aagccgaaac cgaacggtag cggcaccgct gccggacttc cgccgtcgcc
    12301 ccgagggcga ccgggacgaa ctcccgcgtt tcggccgccg cccggagggc cccccggtcc
    12361 cgtccggccg cggcgcagat ccgttcggcc gccccggcgc tgatccagcc ccggaaaaac
    12421 aggcgctgcg gcttgtcggg aaggaaatac tcctcgttgg accatccatt ccggacgacg
    12481 tcccagccgt aagccgcgcc cttcgcgtcg tggatgatga gacagccggc cgccccgagc
    12541 tcggccgctt tctcgtactt gtagacccat cggccgtaat acgtcatcga ttccccgtcg
    12601 aaaaggccgc cgggaacgtt gcccggctca ttgatctcga ccaggaggac tttccccgcc
    12661 aggtccatcc ccttcaggtc atcccagtcc cgttcggggg cttggatgcc gtagccggcg
    12721 tagaccagct cgccggaaac gctcgggggc gcatcctccc ggtaggaatt cacgacgaac
    12781 tcctcccggg gttccaggga aacgttcacc ccgccggccg cgacggacag ggacggcttc
    12841 gggtcggggg cgcattccct cgcctcgaac gtctggcgat agcttccgcc cgggaaggcc
    12901 ggctcgaggc cgagaccgcg gaaaacctgt tcctggtaga gggcggcgat ctcgattccc
    12961 cggcctccga gcgcccggcc ctcgagcagg tcgtcggaga ggaaccggat atgggcttcg
    13021 atttcggccg gggtgatcgg acgcggggcg gaagcgtccg tccggcaggc cggaagagcg
    13081 gccgccaggg cggccgcggc cagaaggaaa aaagcggtct tcgcgcgagt catgtcattt
    13141 ttctcctttg ccgccggcgg aagacggcgg cggacgatcg gggccccggc ggctttcccg
    13201 ttcccgggag gcgaggccga agatttcatg gacgcccgat actccggccg cccgggccgc
    13261 caaggcggcg ccggcctcgg ccgcttcgaa gaccgtgcct ccgcgaagca aggcggcgga
    13321 cgcggccgcg gcgaagacgt cgccgcagcc cgtcgggtcg acgaagaccg gcgcggccgc
    13381 gggcggaatc cggcggacgc tctccggcgt cgaaaccgcc gcgccggccc ggcccagggt
    13441 gacgataacg gcccggacgc cgagacggaa cgcccgccgg gccaattcct cgatttcagc
    13501 ctccccggct tcgcggtccg gccggccgac aagacaagcc gcctcccggc ggttggcttg
    13561 aagataggcc gcgccctcga cccacgcctc ccattcggga accgtccgat acccgcggcg
    13621 ggacccgacg cgcggctcca aggcgaggga atggatgtcc atccagacgg gacacgacgc
    13681 ccgcccggcg acggcgcgcc agtccgcgag ctcgatatcg aagccggaat tgaagaccat
    13741 catcaaaaaa tccaacccgg ggagagcctc gagaaccggg ccggggtcaa gcggcggcac
    13801 gacggattcg aggatctcct cccgctcccg ggagcgcccg gcatatcgga gccggacgcg
    13861 gtttccgggt ccggggacga agcgcaggcc ccgccggtcc aggtccggcc ggccgccgag
    13921 gaccgcttcg acctccggac ggagctcccc gccgcagcgg gaaaagagag ccgccggaac
    13981 gcccaggccc gagagcgccg ccgcttgata aagaatcccg ccgacggacc ggaacgcccg
    14041 gagggtccgg ccgtcctcga tttcaatcgt gtccgccgtg atcgttccga tgaggccgca
    14101 gcaggtcatg agatttcatc ctaggcgagc ccgggatttt tttcaaggaa agagcagggc
    14161 gcggcgatga aggccgagtt cgccttggcg gcgatccaag gggaaaagac gatcgccccg
    14221 attgagggag aggatggaat ccccttcgcc ggattcggat ccggcgggat acagcttgag
    14281 ccgcttttct ggacgatttt tgttttttta tcttctggct catccgacat tcttgtgggt
    14341 aacagggtag aggtttaggc cgccacagtg gaagaaaata gccagcttga agtgctcgcg
    14401 attgcggaag ccgcaggcca ttttcttgat cgtttgaatc atcgagttca cgccttcgct
    14461 gaccgcgttc gttatccgat gtctcaagta agtcataata ttgggcagat atttccggaa
    14521 cattcgggcc accgccttca ccggtttcaa ttgcgagcgg atcgcccatc cataccaacc
    14581 ggaaaagtgt tttctcgccc aggtttccgt ctgatacgac cagaacctcc tcaagtcttc
    14641 ctttatcgcc cacgcgcggg ctgttctcaa gcgcttcctc ctcaaggccg aaaacgcctt
    14701 tcgtttcttg accggaagat tttccctcga atacagccaa agatactttg ttcccgacag
    14761 gatctcgttt ccttcccccc tcaggtcccg atgctcctgt tttcgaacca tatcaacggc
    14821 ttggaccata tgagtcatca agtggtatcg gtcgaaaacg agcttttctt cgtagccggg
    14881 aacatgctct cgaatcgagg ccagataggg atcccagata tcggtggcga tggccttgat
    14941 tttggcccgt tgagccggcg tcatcttgcg aaaatatccg tccaggcttt cttttttcct
    15001 atcatcctcg atatgttcca cggtcgatcg ttgcaggtca taaatcaacg tcagatagtt
    15061 gtgccctttc cccagagatt tttcatccac gcccattttc ttaacaattc cgggcttctt
    15121 cctttcctgg cctctcctca cagcccgctc caaaatatgc caggcctcat cccagctgat
    15181 ccgcaggatt cttcccgccc cctcgatatt cgtttcctgc aagaccatga tcgccagtcg
    15241 ttcaaaaagg gccgtaaatc tcgatttttc ttcggcccag ttgactcgga cctgtttaac
    15301 gccgtgggcc ggacattcca cccggggaat ccgggcatga agaaatgttt tgaactggca
    15361 ggtgtccaga tgccgccaga cacgttccgg agcgtggtca taaatcggaa gcggcgttcc
    15421 acattccggg caggaaaagc ggatgctttc cccatgaacc gcccaaacgt cgacctgctc
    15481 ttccctcacg ctcaattgaa ctcgatccac ccgccacgga tcttcaatcc ccaaaacatg
    15541 ccgataaagc tccgtatctt gcatggctcc attctcctcg gagccatttt atcccagacg
    15601 agtacccatt aaaatgtcgg atgagcctat cttcttcctt ctggaaaaga ccgttctttt
    15661 gttgaatagg gatttctggg gaaccggcat gttgcgagaa aacggcgatt aagaatataa
    15721 gcgtttttat tcatgacctc acctttcctg taaatatctt tgcacgactt ctctgaggaa
    15781 ttccggatca aaattcgaag ccatctgtcg gctgagataa agaatacact tcgcttttaa
    15841 agactcgttt ttattggaag tattcccgcg ataaacgaac ggaagaatgg agaaaaccgc
    15901 cgttacgtcc ggatgaagaa atgcgtgtcc gtccgctcct cgctccggtc ggtcgccgga
    15961 ggcggggccg gcgagagggc cgagatctcc gcccggaggc cgtcgtccat cgggatctcc
    16021 agggatccca aaacatcgcg aagctgggcg aggttgcggg ccccgatgat cggggcggtg
    16081 acggccgggt gcccgcccgc ccaggcggcc gccagggcgg ccggagacca cccccgggcc
    16141 cgggcgaatt cgcagaaccg gtcgacgatc tcgaaattcg cctcctcgaa atagcgcgcc
    16201 tggtagatct tgtgttcggt caaacggccg gatgccggcc gccggttcga gccgaatttc
    16261 ccgctcagaa agcctccggc cagcgggctg taggggatca cgcccaatcc ctccgcctcg
    16321 gccatgggaa ggatttcggc ctcggcctgg cgtttgagaa ggttgtacat cggctggagg
    16381 acgcccggcg gcgcccaccc gtggagagct gaaaggccga gggctttctc ggcctgccag
    16441 gcggcgaaat tgctcaggcc gggatagagg attttccctt ggcggacgag gtcgtccagc
    16501 gcccggagcg tttcctcgag cggcgtgcgg tcgtcgaagc ggtggatgaa atagacgtcg
    16561 atgcggtcgg tccggagccg ccggaggctg gcttcgaccg cgttccggag atgatagcgc
    16621 gaggcgccct gggcgttggg attcgttccc gtcgggaaaa aacccttcgt cgtgacgacg
    16681 acttcttccc ggcggtcctc gatcagccgg ccgaggattt cctcggcccg gcccgcctgg
    16741 tacatgtcgg cgcaatcgaa aaagttgatg ccggcctcgc ggcaggcgcg gaacagctcc
    16801 ccggaaaccg cctcgtcggc ttccttgccg aaggtcatcg tgccgaaaca caaggacgag
    16861 acgcggacgc cggtccggcc gagatatcga tagttcatga ctcgctcctc tccgccggac
    16921 ggccccctcg agcggccgcg gcgatcatcg aaaacggagg gacacgcgga gaccggccgg
    16981 gctcaatcgc cccgccgccg gaggatgttc atgatcaaag cgctttcccc gctcataaag
    17041 gtcagctgga tgaccatgtt ctccctctcc agactggtga atgcgccctg ggggacgtcc
    17101 gactccagga caaggctcca accgtcgccc ttcaggtcct cgatgtaggc cgcga
//
LOCUS       DAOOXH010000051        16985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_3280_length_16985_cov_7.964678, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000051 DAOOXH010000000
VERSION     DAOOXH010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 16985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16985
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3280_length_16985_cov_7.964678"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="PKV64_07195"
     CDS             complement(<1..1168)
                     /locus_tag="PKV64_07195"
                     /inference="COORDINATES: protein motif:HMM:NF020086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPN16900.1"
                     /translation="MNGLRPPRRDVVLITGEIHVDHPLSASGVIARVLEAEGRTVGII
                     EHPDWKTDADFLQWGAPRLFFGITSGSIDSLLANSTPLKRRREKDPHAPRKTAMPDRA
                     VLVYANKIRRLFPGTPIVLGGIEASLRRFAHYDYWENRVRRSLIVDARADILVYGPGE
                     KAAVEIARRLEEGLDLQGIAGTCVVSRNVPADFVLLPPAEAVAGSPDAFAEAQMKLSV
                     RTPLAQGHAGRFVLQYPAPRWTPADLDRIYGLPYSRLIPPDFPEFGMARFSVVSHRGC
                     FGRCSFCALALHQGDTIVSRSEESILEEIRRLVRHPEFKGSIDDLGGPTANMYGMDCE
                     AACGESCLDCGRRPGHERLISLLRRAREIPGVRNVFVRSGVRYDLALSSEEYIRE"
     gene            complement(1188..5882)
                     /locus_tag="PKV64_07200"
     CDS             complement(1188..5882)
                     /locus_tag="PKV64_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN16901.1"
                     /translation="MGGASLRDRFPFFFVPQIRPSTDAGPGRRGREKIRLISSVVSIL
                     VLIGGAAVAPLRAEAPPRRFGNDFVRLSVNEGMSQGSGISIVQDRPGFIWIMTEDGLD
                     RYDGRRFRVYRVTEEPASLSDVTGSVLFEDSGGILWVGTNSGLNRYNREQDDFTRYVH
                     DPDDPKTISHNMVYSLCEGRSGDLWVGTENGLNAFDPKTGTFTRYIRTSRRRVPSSPA
                     AIRSLVEDAEGVLWMATNGGLHAFDRARGRVKRYVHDPDDPRSLSRDEVNTLFLDEDG
                     TLWVGTNGGGLNALNPKTGTFTRYLPDFRDPRSLSHAVINVIYRDRAGRLLVGTYDGL
                     NIFDEETKDFTVIRHDPADPDSLSSSNIISIIEDRTGVYWIGTRGRGVNKYVPDRNKF
                     HKLQEIHGGRKELISNLIRSFAEDEAGNLYLASEDKGLEYWNRTTGAHAFIRHDPGNP
                     SGLSDDAVYALSLDEGGALWIGTLGGGLNRYDPRTESFQHWRRDSRDPSSLSHDSIRC
                     LLRDRAGALWVGTDGGGLNRFLPEKNAFVRYPHDPARPDSLGFDLVRAIFEDASGTLW
                     IGLAGGGLDRFDRARETFTHFRKRPGDPGSLSNDYVMAITQDSSGALWIGTADGLNQF
                     DPRTETFVKYGVAEGLPNAMIYALQIDEGGQIWGSTNAGLFRLNVRTGAVKSFDVSDN
                     LQGNEFLGGSSFKTKAGEMFFGGVSGLNYFFPSDIVDNPFPPDVVLTDFQIYNKSVPI
                     GREVEGRVVMAQAIWSTPEIELTFRDRLITFEFASLHYAAPEDNVCAYMMEGLEEDWI
                     TVKERRFASYTNLPPGRYVFRVRASNSDGLWNNDGVALRIRVIPPFWRTWWFLGLAGT
                     AILAILGAYITTGIQRNRRRTKFLESRVRERTAELETQIAVREAAERELDKRRQYLES
                     ILLNAPNAIITVDPKGRITEWNPGAERLFGWAREEAQGKEADGLIVAKDLQGEAARLT
                     EMTFAGTALPPRESVRLHKDGRPVPVLFGGSPIRSGGEIVGTLFVYTDITEIKKAEEA
                     AQEANRVKSEFLANMSHEIRTPMNGVFGMTELALETDLTAEQREYLEAVQYSAASLMG
                     ILNDILDYSKIEARKIEMESIPFRLRDTIHIMIASVALLAEKKGLELAYHVAPDVPDR
                     VKGDPGRLRQVLTNLISNAVKFTSAGEVIVTVGVEERLPDRVKLHFQVSDTGIGISPD
                     KLRSIFAPFTQADSSATRLFGGTGLGLAITAQLVELMKGKIWAESVLDRGSTFHFIVP
                     LDLQTQPEEETAPSRYEDLLDLPVLVVDDNAANRMILNDLLTQWGLKPAQAESGEKAL
                     DMLRERAAAGRPFRFVITDANMPRMDGFALAAAVKTMPEYADVQIMMLSSAGLRGDSA
                     HCRKLGLTAYLTKPVKPAVLLDAILAAVGISPEKRAQAPLITRHSLGRSPARYEILLA
                     EDNIINQKLAVRILENQGHRVTVAGNGAEALEALNRGRFDAVLMDVQMPVLDGIQATT
                     EIRRREMATGAHIPIIAMTAHAMTGDREKCLAVGMDDYVSKPLKPLDLMKTIQRAVDR
                     ITRERRGIPREP"
     gene            complement(6012..6962)
                     /locus_tag="PKV64_07205"
     CDS             complement(6012..6962)
                     /locus_tag="PKV64_07205"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPN16902.1"
                     /translation="MKRIGMNKAVVIAVFGVFLLAGSLAARDTYEEKFDRTEALDRNG
                     RVSLENISGTIEVRSWDKAEVRIDALKVSKASSRDQAKENAGLVRIEVVKEGGVLRIE
                     TKYPERRFRGNNLSVSVNYVLTIPDQASLRVKNVSGDIDVQKIGGDLDIDEVSGGVRI
                     AGAAKTVDCKTVSGGITLRGAGGAVSLKAVSGGIRAEDVKGSIEAETVSGGITLGNVR
                     EASSVRAKTVSGGIDCETEILPSGRYSFDALSGGITLLLPASAAFEVEAETFSGHIAT
                     DFPVVVSGKISPKELRGTVGTGGATVRIKSFSGRVEIRKK"
     gene            complement(6983..7693)
                     /locus_tag="PKV64_07210"
     CDS             complement(6983..7693)
                     /locus_tag="PKV64_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPN16903.1"
                     /translation="MKCTQAHELIGELLEGTIREEDRRALEEHLESCPDCRELLADFQ
                     EIKTQAAALPRIEPSPAVWPNILDAVRRERRERSARAVLSVSWWDRLFAPGRPRYALA
                     AALALVLVVGGALLILPSRKPAAPAVSSGNGGEYAMAKLQEAEIHYRLAIRALSDAAA
                     GQQTGLDAKTMEALDRDLRTIDAVIASCREAVLRDPRNVEARVYLLGAYKGKVEFLEN
                     IIEAKKKSTAPLSGKIAL"
     gene            complement(7703..8347)
                     /locus_tag="PKV64_07215"
     CDS             complement(7703..8347)
                     /locus_tag="PKV64_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPN16904.1"
                     /translation="MIQEAALSPSLEFGDNAFVRYSRRETGVRMIDIESEELADLIIQ
                     ARAGETEAIEEIYRRFKTGLFNLAFRYSYDRATAEDLLQEIFIKVFTHLDNVKNPDTF
                     AGWVFRIGLNTCYSHLRSRRVEMEKGIPLEDIEGTRCGATEENPARDMRRPLDDGIAA
                     LPGKLREIFLLHDVHGFKHSEIARMLGLSVGTSKSQLFKARLRMRDFLERKGVC"
     gene            8469..9257
                     /locus_tag="PKV64_07220"
     CDS             8469..9257
                     /locus_tag="PKV64_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HPN16905.1"
                     /translation="MKSLSSRPIGPFSAVSMLILLVLSVFLPACRPRARVLPLGPAAG
                     PGGGPPPGYQVQAGAFSLEDNAARLTRSLREQGWNAFYLFHETRVFKVRIGPFRTRKE
                     AVEAADALLGRRLIADFLIIPPGSEIIPGPAGGTEDDLRDRLASTALGFVEFPYVWGG
                     ASPEEGFDCSGLAMTVYRINGLFLPRALTEQAAAGRAVPAGRAKKGDLIFFSMDKTVR
                     ASHVGILIGGNRFVHAPGREKAIRPDSLANPYFRDRLLEIRSYL"
     gene            complement(9660..9735)
                     /locus_tag="PKV64_07225"
     tRNA            complement(9660..9735)
                     /locus_tag="PKV64_07225"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9700..9702),aa:Ala,seq:cgc)
     gene            9897..11243
                     /locus_tag="PKV64_07230"
     CDS             9897..11243
                     /locus_tag="PKV64_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HPN16906.1"
                     /translation="MRKASRFAFAAAAAAPFLVLLLAAPPPEKKSYDAFETPRRCAQC
                     HREIHVQWKQAMMSQAFTHPWDEIEYFQLAVPQAEKDPKVAEVKAGCNGCHAPSAFFI
                     GDVPPPRPAAGSRANESVFCDICHTITGKSGDVPVNFNYVLSPGRTKHGPREGVVSPY
                     HETRYSDFVRSPEFCGTCHNELNPYGLYVKSTQLEWQDGPYSRQGVRCQDCHMWHAPG
                     TSADMGSPVEDMARHTFPGAHVPSKVRGAIEMKIYADADELEPGEKAVISLHLYNAKA
                     GHKIPTGSVEDRQLWVEVHAVDAAGIRYALPVDKKGFPDEEYTITSNAPAYQDMGDMM
                     MDLPGFPGVSRDAVAEEGHRVFRMPYFDPKGRMTIGQWNTASFGTDYRIGPRETKIET
                     YSWTLPQTLPEGPLRIEAKLYYRLLVKSVADFLKVPPEESAALEVNSAETVLQVVF"
     gene            11254..12399
                     /locus_tag="PKV64_07235"
     CDS             11254..12399
                     /locus_tag="PKV64_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16907.1"
                     /translation="MKKIFAFLVLVAGLVQAAAAIPAFARKYSYSCDVCHAPVPRLKA
                     FGEEFMDNGYRLPDNEPPRATIDTGDPLLLLQRTLPLAFRFDAYLAYEPEADITGDFR
                     APLAMKILSGGNISDDISYYTYFLMAEDGKVAGLEDTFLSFRNVFGLPLDMMFGQYRV
                     SDPIKATETRLTVENYALFKFRVGDSRINLAYDRGLLLSTKTGFGLDVVAQIVNGNGI
                     EEQEIFDSDKYKSVVGRLAQSFAGGRVRVGVLGYAGKEAGAGGAVNAVTYLGPDLLLR
                     LPNVEILLAYVKRTDSNPLFQREGREQTSEAVLAEAILSPWGEQGRTFFTLAWNRVNS
                     TLEADDANALTVNVSRLIRRNLKWTAEYTRDFERKEDRWLTGIVTAF"
     gene            12422..13480
                     /locus_tag="PKV64_07240"
     CDS             12422..13480
                     /locus_tag="PKV64_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HPN16908.1"
                     /translation="MNEKKRGARPRWISAAVLIPSFLGALACSGPPPPAGSSHPAGAA
                     ALFSRERVTIAVTDSGLGGLSIMAEAASRMKAARAFRGVDLVFWNALFSNDSGYNSLP
                     SREDKVRIFDASLRSLAAEVKPDLILVGCNTLSVLLGDVPFARTADVPVLGIVDTGVE
                     MMAEALSRNPRAAGLLLGTATTIAEGEHRRKLLAAGIEEERLVSQPCPDLVGLIESDW
                     RGEEAARLIETYIGEAADRLPDAGAPVYAGLFCTHFGYASALWERAFARRGLTLAGLV
                     NPNSRWVDALDPPARRNRFPETEVRARVISMVEIPAAVRDALGEWLRPLSSETEDALK
                     NYDLRPGLFEWKSLLKSD"
     gene            complement(13666..14658)
                     /gene="purM"
                     /locus_tag="PKV64_07245"
     CDS             complement(13666..14658)
                     /gene="purM"
                     /locus_tag="PKV64_07245"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPN16909.1"
                     /translation="MTYRRAGVNIAAADDFLRRIKPLVRTTSRPEVLGGIGGFSGLFL
                     PRLKGLKEPLLVSSTDGVGTKIMIADILGRYDTVGIDLVAMNVDDVVVTGAEPLFFLD
                     YIACGKVKPAMLTQVVKGIVRGCREAGCALLGGETAELPGLYGKDQWDLAGFCVGLVD
                     KSRLIDGRKCRPGDAVVGLASSGLHSNGYSLARRVFTKKELAGPFGRTLLRPTRIYAR
                     LLLEAMAAIDIKAMAHITGGGFYDNIPRVIPAGCGVRIQKRSWPVPRIFRVIQERGGV
                     AEAEMYRTLNMGIGMTAVVKAADAGRMIRWLAARGQRAWVIGELTRDSSGVVVE"
     gene            14793..15737
                     /locus_tag="PKV64_07250"
     CDS             14793..15737
                     /locus_tag="PKV64_07250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HPN16910.1"
                     /translation="MGGKRKKEKIVVIGGGTGSYNVLCGLKKYPVDLTAVVTMMDSGG
                     STGRLRDEFGFLPPGDVRRCLVALSSDTGLLRKLFEYRFDRGSGLSGHSFGNLFLTVL
                     RDLTGGDAAAVREAAKILNVRGQVLPVTLNNCQLGVVLENGQTVVGETNISIPRHDPR
                     LRIRSLFVVPRARINKEAEAAILEADKIIIGPGDLYTSILPNIAVSGVKAALRKTRAR
                     VIYVCNIMTKAGETLGFTAVDFVRELEHYLGRGIVQDIVCNDQKPPARMLKRYAAERA
                     EFVEPDLEEGRVRRASLLGGRDLARHDSDRLARVLMSL"
     gene            complement(15764..>16985)
                     /locus_tag="PKV64_07255"
     CDS             complement(15764..>16985)
                     /locus_tag="PKV64_07255"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPN16911.1"
                     /translation="NILMFRAENVPSIGYAVFTAAPGEPSAPAEAGAVIEDNRIENRF
                     FQVTADPVTGGLRSLVDKETGRELVDPASAFGLNQLIYENPEGGREAVDDRSKRAAFR
                     RYFPESAAVSAGFRGPVASSLVLSSRMFRFPEIRQEIILYDDLKRIDFINRMRKEETT
                     EAEAAYFAFPFRVENGRLRLEIADAVMSPEADPLPGTTRDWLAVQNWIEAAGTKNTVV
                     WAPLEAPLVQLGDINTGKWQAKMDVARPFVFSYVLNNYWMTNFKASQGGPLVFRYALT
                     SRPGGSDQVAAARFGAEARTPLLAEWLPNQTAGVLPADSLSFFAVDQPNVLIQTVTAP
                     ESGEGIVLRLREIAGRETRTRVSSSLFTAETLTYTATDIGEHPANAFEVVPRSIYVDL
                     KPFQILTVKIRSPR"
BASE COUNT         3021 a         5481 c         5345 g         3138 t
ORIGIN      
        1 gctcgcggat gtattcctcg ctggacaggg ctaagtcata gcgaaccccg ctccggacga
       61 agacgttccg gacgccgggg atctcccggg cccggcgcaa aagcgagatc agccgttcat
      121 gtccgggacg gcggccgcag tccaagcagg actctccgca ggccgcttca caatccatcc
      181 cgtacatgtt ggccgtgggc ccgcccaggt cgtcgatcga tcctttgaac tcgggatgac
      241 ggacgagccg ccggatttcc tccaggatcg attcctcgga gcgcgagacg atcgtgtcgc
      301 cctgatggag ggccagggcg cagaaggagc accggccgaa gcatccgcgg tgggagacga
      361 ccgagaaccg ggccatcccg aactcgggga aatccggcgg aatgaggcgg ctgtacggca
      421 gaccgtaaat ccggtcgagg tcggccggag tccagcgcgg ggcgggatac tgaagaacga
      481 atcgtccggc gtggccctgg gccaacggcg tccggaccga gagtttcatc tgggcctcgg
      541 cgaaggcgtc cggggagccg gccacggcct cggccggagg aaggaggacg aaatccgcgg
      601 gaacgttccg ggagacgaca caggttccgg cgattccctg caaatcaagt ccttcttcca
      661 gacgacgggc gatttcgacg gccgccttct cgccgggccc gtagaccagg atgtccgccc
      721 gggcgtcgac gatcaggctg cggcggaccc ggttctccca gtaatcgtaa tgggcgaacc
      781 gcctgagcga ggcttcgatt ccgccgagga cgatgggcgt tccggggaag agccgccgga
      841 tcttgttggc gtacacaaga acggcccgat cgggcatggc cgtcttccgg ggcgcgtggg
      901 ggtctttctc ccgccgccgc ttgagaggcg tggagttggc caacaggctg tcgatcgagc
      961 ccgaggtgat cccgaaaaac agccgcgggg cgccccattg gagaaaatcg gcgtccgttt
     1021 tccaatcggg gtgttcgatg atcccgaccg tgcggccctc ggcctcgaga acccgggcga
     1081 tgacgcccga ggcggaaaga ggatgatcga cgtggatttc gccggtgatc agaacaacgt
     1141 cccgtcgcgg cgggcggagg ccgttcattt caagtccggg cgggagctca gggctccctc
     1201 gggatccccc gccgctcccg ggtgatccgg tccacggcgc gctggatggt tttcatgagg
     1261 tcgagcggct tgaggggttt ggagacatag tcgtccatgc cgacggcgag gcatttttcc
     1321 cggtcgccgg tcatcgcatg ggccgtcatg gcgatgatgg ggatatgggc ccccgtggcc
     1381 atctcccgcc gccggatttc cgtcgtcgcc tggatcccgt ccagcacggg catctggaca
     1441 tccatcaaga cggcgtcgaa ccggccccta ttgagggcct ccagcgcttc ggctccgttg
     1501 ccggcgaccg tgacccggtg gccctggttc tccaggatgc ggacggccag cttttggttg
     1561 atgatgttgt cctcggccag gagtatctca taccgggcgg gagagcggcc caaggaatga
     1621 cgggtgatca agggggcttg ggcccgcttt tcgggggaga tccccacggc cgccaggatg
     1681 gcatccagaa gaacggccgg cttgacgggc ttggtcagat aagccgtcaa acccagcttc
     1741 cggcaatggg ctgaatctcc gcgcagtccg gccgagctca gcatcatgat ttggacatcg
     1801 gcgtattccg gcatcgtctt gacggccgcg gccaaggcga acccgtccat gcggggcata
     1861 ttggcgtcgg tgatgacgaa gcggaacggc cggccggcgg ccgcgcgctc gcgcagcatg
     1921 tccagggctt tctcgccgct ctccgcctgc gccggcttca gtccccattg ggtcaggagg
     1981 tcgttcagga tcatccggtt ggcggcattg tcgtccacca cgaggacggg aagatccaac
     2041 aggtcttcat aacggcttgg agcggtttcc tcctccggct gcgtctgaag atccaggggg
     2101 acgatgaaat gaaacgtgct tcccctgtcg agcacgctct cggcccagat ttttcctttc
     2161 atcagctcca cgagctgggc ggtgatggcc aggccgagcc ccgtccctcc aaaaagccgg
     2221 gtggcggagc tgtcggcttg ggtgaacggg gcgaagatcg atcggagctt atcgggggaa
     2281 atgccgatgc ccgtgtcgct cacctggaaa tggagcttca cccggtcggg aaggcgctct
     2341 tccacgccga ccgtcacgat cacctcgccg gcggacgtga acttgacggc attggagatc
     2401 aggttggtga ggacctggcg cagacgtccg ggatccccct tgacccggtc tggaacgtcc
     2461 ggggcgacat gataagccag ctccaatcct ttcttctcgg ccaggagggc gaccgaggcg
     2521 atcatgatgt ggatcgtatc ccgcaggcgg aaggggatcg attccatctc gatcttcctc
     2581 gcctcgattt tcgagtagtc caggatgtcg ttgaggatcc ccattaagga tgcggccgaa
     2641 tactggaccg cctccaggta ctcccgctgc tccgccgtca gatccgtttc cagggccagc
     2701 tccgtcatcc cgaaaacgcc gttcatcggc gttcggatct catgactcat gttcgccagg
     2761 aattcgctct tgacccggtt ggcttcctgg gcggcttcct cggccttctt gatctcggtg
     2821 atgtccgtgt acacgaacag cgttccgacg atttcgccgc cgctgcggat cggcgatccg
     2881 ccgaacaaaa cggggaccgg ccggccgtcc ttgtgcaggc ggacgctttc ccggggggga
     2941 agcgccgtcc cggcgaaggt catctccgtc aggcgggcgg cttcgccttg gagatccttc
     3001 gcgacgatca agccgtcggc ttcctttcct tgcgcttcct cccgcgccca gccgaagagg
     3061 cgttccgcgc ccggattcca ttcggtgatc cggcccttgg gatcgacggt gatgatggcg
     3121 ttcggagcgt tgaggaggat cgattccagg tactgccgcc gcttgtccaa ctcccgctcg
     3181 gccgcttccc ggaccgcgat ctgcgtttcg agctcggccg tccgttcccg gacccgggat
     3241 tccaaaaatt tcgtccgccg gcggttccgc tggatgcccg tcgtgatata cgcgccgaga
     3301 atcgccagga ttgcggttcc ggccaacccc aggaaccacc atgtcctcca gaacggcgga
     3361 atgacgcgga tccgcaaggc gaccccgtcg ttgttccaga gcccgtcgga attggacgcc
     3421 ctgacccgga agacataccg gccgggcggg aggttggtat agctggcgaa cctccgctcc
     3481 ttgaccgtga tccagtcctc ctccagtcct tccatcatat aagcgcagac gttgtcctcg
     3541 ggcgccgcgt aatggaggga ggcgaattcg aacgtaatca gccggtcccg gaatgtcagc
     3601 tcgatctccg gcgtgctcca gatcgcctgg gccatgacga cccggccttc cacttcccgc
     3661 ccgatgggca cggatttatt gtagatctgg aaatcggtca ggacgacgtc cgggggaaaa
     3721 ggattgtcga cgatgtcgct cggaaagaaa tagttcaagc ccgagacgcc gccgaagaac
     3781 atctcgccgg ccttcgtttt gaacgaggat cccccgagaa attcgtttcc ctggagatta
     3841 tccgacacat cgaacgattt gacggcccca gtgcggacgt tcaagcggaa gaggccggcg
     3901 ttggtcgaac cccagatctg gccgccctcg tcgatctgaa gagcgtagat catggcgttg
     3961 ggaaggccct cggcgacccc atacttgacg aaggtctccg ttctcgggtc gaattgattc
     4021 agtccgtcgg ccgtcccgat ccaaagcgcg ccgctggaat cctgggtaat ggccatgaca
     4081 taatcgttgc tgaggcttcc cggatcgccg ggccgtttcc ggaaatgggt gaaggtttcc
     4141 cgggcccggt cgaaccggtc caggccgccg ccggccaagc cgatccagag cgttccggag
     4201 gcgtcctcga aaatcgcccg gacgaggtcg aagccgagcg aatccggccg ggcgggatca
     4261 tgcgggtaac ggacaaaggc gtttttttcc ggaaggaacc ggttcaatcc gccgccgtcg
     4321 gtgccgaccc acaatgcgcc ggcgcgatcc cgcagcaagc aacggatgga atcatggctc
     4381 aggctggagg gatcccggga gtcgcggcgc caatgttgga acgactcggt cctcgggtcg
     4441 taacggttca atcccccccc gagcgtcccg atccaaagcg ccccgccttc gtcgaggctc
     4501 aaagcataga cggcatcgtc gctgaggccg gacgggttgc cgggatcatg gcggatgaag
     4561 gcgtgcgcgc cggtcgtccg gttccaatac tccaggcctt tgtcctcgga ggctaaatac
     4621 aagttccccg cctcatcctc ggcgaacgac cggatcaagt tggagatgag ctccttcctc
     4681 ccgccatgga tctcctgaag cttgtgaaac ttgttccggt ccgggacata tttattgaca
     4741 ccccgtcccc gcgtgccgat ccaatacacg cccgtccggt cctcgatgat agatataatg
     4801 ttggaagagc tgagactgtc cgggtccgcc ggatcatggc ggatgacggt gaaatccttt
     4861 gtttcctcgt cgaaaatgtt caatccgtca taggttccga ccaggaggcg gcccgcccgg
     4921 tcccggtaaa tgacgttgat gacggcgtgg ctcaagcttc ggggatcgcg gaaatcggga
     4981 agatatcggg tgaaggttcc tgttttcggg ttcaaggcat tcaatccgcc gccgtttgtc
     5041 cccacccaaa gcgttccgtc ctcgtccagg aacagcgtgt ttacctcgtc gcggctgagg
     5101 ctgcggggat cgtccggatc atggacatag cgcttgaccc ggcccctcgc ccggtcgaag
     5161 gcgtgaagcc ccccgttcgt cgccatccag agaacgcctt cggcgtcctc gaccagggat
     5221 cggatcgccg cgggcgacga gggaacccgc cggcgggatg tccggatata ccgggtgaat
     5281 gttccggttt tcggatcgaa cgcgttcagg ccgttttcgg ttccgaccca aaggtccccg
     5341 gaccggcctt cgcagaggga atacaccatg ttgtggctga tggttttcgg gtcgtccggg
     5401 tcatggacgt agcgggtgaa gtcgtcctgt tcccggttgt agcggttgag gccggagttg
     5461 gtcccgaccc ataaaatccc cccggagtcc tcgaacagaa cggatcccgt cacgtcgctc
     5521 aagctcgccg gctcctcggt cacgcgatag acgcggaagc gtcggccgtc gtagcggtcc
     5581 aggccgtctt cggtcatgat ccagatgaat cccggccggt cctgaacgat cgatatcccg
     5641 gaaccctggg acatcccttc gttgacggaa agacggacga aatcgttccc gaagcggcgc
     5701 ggcggcgcct ccgcccggag gggcgcgacg gccgctccgc cgatcaaaac caggatggaa
     5761 acaacggacg aaatcagccg aatcttttcc cggccgcgcc gccccgggcc ggcgtcggtc
     5821 gaaggacgga tttgagggac gaaaaaaaac gggaatcgat cacgtaaaga agccccgccc
     5881 atgtccaatc ttccgccaag agtgtatctt cggggagaga atcttgtcaa tcttattttc
     5941 cggtcggacg gtaaaaaaaa gcgcctccgg acgatgtccg gaggcgcaac ggcctccatc
     6001 cggccggagg attacttttt ccggatctcc acccgtccgc tgaagctctt gatccggacg
     6061 gtcgccccgc cggtcccgac cgtcccccgc aattccttgg gggaaatctt gccggacacg
     6121 acgacgggga aatcggtcgc gatatggccg ctgaaggtct cggcctcgac ctcgaaggcg
     6181 gccgacgccg ggagcaggag cgtgatcccg ccgctcaggg catcaaaact gtagcggccc
     6241 gacggcagga tttcagtttc acaatcgatc ccgccgctga cggtcttggc ccggacggag
     6301 gaggcctccc ggacattgcc gagggtgatg ccgccgctga cggtttcggc ctcgatggag
     6361 cccttgacgt cctcggcccg gattcctccg gaaacggcct tgaggctgac cgcgccgccc
     6421 gcgccgcgga gggtgatccc gccgctgacg gtcttgcaat cgaccgtttt ggcggccccg
     6481 gcgatccgga cgccgccgct cacctcgtcg atgtcgagat cgccgccgat cttctggaca
     6541 tcgatatcgc cgctgacgtt cttcacccgg agggacgcct ggtcagggat ggtgaggaca
     6601 tagttcacgc tgacgctgag attattgccg cggaacctcc gttcgggata tttcgtctcg
     6661 atccgcagga cgccgccttc cttgacgacc tcgattctca ccaggccggc gttttccttg
     6721 gcttgatccc gggaggacgc cttggaaacc ttgagggcgt cgatccggac ttcggccttg
     6781 tcccaactcc ggacctcgat ggttccggag atgttctcca gcgaaacccg gccgttgcga
     6841 tccaacgctt cggtccggtc gaatttctcc tcgtacgtgt ctcttgcggc cagacttccg
     6901 gcgagaagga agacgccgaa aacggcgatg acgacggctt tgttcattcc gattctcttc
     6961 atggctgttc tccttcctct cttcaaaggg cgatcttgcc gctcagggga gcggtcgatt
     7021 ttttcttcgc ttcgatgatg ttttcaagga actcgacctt gcccttgtac gcgcccagga
     7081 ggtagacgcg ggcctcgacg ttccggggat cgcggaggac ggcctcgcgg caggaggcga
     7141 tcacggcatc gatcgtccgg aggtcccggt ccagggcttc catcgttttc gcatcgagcc
     7201 cggtctgctg cccggccgcg gcgtccgata gtgcccggat cgccagccgg taatggattt
     7261 cagcctcctg gagcttggcc atcgcgtatt cgccgccgtt tccggatgac acggccggag
     7321 cagcgggctt ccgggaggga agaatcaaga gagcgccccc gacgacgagg acaagagcca
     7381 aggccgcggc cagggcatag cggggacggc ccggagcgaa taaccggtcc caccaagaca
     7441 cgctcagaac cgcccgggcg gatcgctccc tccgctcccg gcggacggcg tcgaggatgt
     7501 tcggccagac ggccggcgac ggttcgatcc gcggaagagc cgcggcttgc gttttgatct
     7561 cctggaaatc agccagcagc tcccggcagt ccggacagga ctcgagatgt tcctcgaggg
     7621 cccggcgatc ctcttccctg atcgtgcctt cgagcagctc gccgatcagc tcatgggctt
     7681 gagtgcactt catggtcgtc tcctagcaga cgcctttccg ctccagaaag tcccgcatcc
     7741 ggagccgggc cttgaaaagc tgggacttcg acgtcccgac ggaaagaccg agcatccggg
     7801 cgatttccga gtgtttgaat ccgtggacat catggaggag gaaaatctcc cggagcttgc
     7861 ccggcagggc ggcgattccg tcgtccagcg gccggcgcat gtcgcgggcg ggattttcct
     7921 cggtcgcgcc gcagcgggtg ccttcaatgt cctccagggg gattcctttc tccatttcaa
     7981 cccggcggct ccggagatgg ctgtagcatg tgttcaaccc gattcggaaa acccagccgg
     8041 cgaacgtgtc cggattcttg acgttatcca aatgggtgaa gaccttgatg aagatttcct
     8101 ggagaaggtc ttcggccgtc gcccggtcat aggaatagcg gaacgccaga ttgaacaagc
     8161 cggttttaaa acggcgatag atctcctcta tcgcctcggt ctcccccgcc cgggcctgaa
     8221 tgatcaggtc ggccagctct tccgattcga tgtcgatcat ccggacgccc gtttcccgcc
     8281 tggaatacct cacgaaggca ttatcgccga attcaaggct gggggaaagc gccgcttcct
     8341 gaatcatcgg gtttctctcc acaccgccat agatgatcaa tccagggaaa aggttgcctt
     8401 ttccgcaaac atttcatccc cgggaaggaa aaggtacaat agagggcaga gaagcggctc
     8461 atccgaccat gaaaagcctt tcgtcccgcc ctatcggccc gttttcggcc gtttccatgc
     8521 tgatcctgct tgtcctttcc gttttcctcc cggcttgccg gccccgggcc cgggttttgc
     8581 ctctcggccc cgcggcgggc cccggcggcg gcccgccgcc gggttaccaa gttcaggccg
     8641 gcgccttttc cctcgaggac aacgccgccc ggctcacccg gtctctccgg gagcaaggct
     8701 ggaacgcctt ctatcttttt cacgaaacgc gggtgttcaa ggtccggatc gggccgtttc
     8761 ggacgcggaa ggaggcggtc gaggcggccg acgcgcttct cggccgccgt ctcatcgccg
     8821 atttcctgat cattcccccc ggaagcgaaa tcatccccgg gccggccggc ggaacggagg
     8881 acgacctccg cgaccgcctg gcctccaccg ccctcggttt cgtggaattt ccctatgttt
     8941 ggggaggcgc ctcgcccgag gaagggttcg attgcagcgg cttggcgatg accgtctacc
     9001 ggatcaacgg ccttttcctc ccccgggctc tgaccgagca ggccgcggcc ggtcgggccg
     9061 ttcccgccgg ccgggcgaaa aaaggggacc tgatcttttt ttccatggat aaaacagtcc
     9121 gggcgtccca cgtcggaatc ctcatcgggg ggaaccggtt cgtccacgct ccgggaaggg
     9181 aaaaagccat ccgtccggat tcgctggcga atccctattt ccgggaccgt cttttggaaa
     9241 tccggtccta tctttaaccg caggcgccgc ggccgcccgt tccgtttttg tcggaaaacg
     9301 gaattttgga aatcccttcc gccgggtccg ccgcgccttt ggaagacgag acgaaaggcc
     9361 gcccctcctc tgtttcggac ctcgaggatc tcgagaccgg acgcccaggg ggccgcggcg
     9421 gcgatccgct tgcttgccca ggagatgaga atcctccaga acggcgatgg ccgtcacggg
     9481 catgggacgg tttcgccttt cgggcgccga aggaagcggg acgaaatcga tgacatcctc
     9541 gacgatgcgg ccgcccgcgt cgattcggaa acagtcaatc gaacggcggc ccggaccgag
     9601 gggaagccga cgggccggac aattcgtaaa aaaagaaaca gcgaacggag gaaaaaggat
     9661 ggtggacgtt gggggattcg aacccccggc ctcagcgatg cgaacgctgc gctctcccag
     9721 ctgagctaaa cgcccacgtc gggtgcggat accggggata tcctagcatg ttcggctgaa
     9781 atttgccacc agttcggacg ggttcccgga agggggacat gcggctttcc gcatgcattt
     9841 gcattcggcc gatttttccg gtatgaatat tttcgacact ccggcgggag gtgcctatga
     9901 gaaaagcgtc ccgtttcgcg ttcgcggccg cggccgcggc ccccttcctc gtccttcttc
     9961 tcgcggcccc gcccccggag aaaaaatcct acgacgcctt cgagacgccg cgccgttgcg
    10021 cccaatgcca tcgggagatc cacgtccaat ggaagcaggc gatgatgtcc caggctttta
    10081 cccatccctg ggacgaaatc gaatactttc agctggccgt cccccaagcg gaaaaggatc
    10141 ccaaggtcgc cgaggtgaaa gccggctgca acggctgcca cgccccctcg gcctttttca
    10201 tcggggacgt tccgccgccg cgtcccgccg ccggaagccg ggccaacgaa tccgtctttt
    10261 gcgacatctg tcatacgatc accggaaaaa gcggggacgt cccggtcaac ttcaattacg
    10321 tcctctcccc cggccggacg aagcacggcc ctcgggaggg cgtcgtttcg ccctatcacg
    10381 agacgcggta ttcggatttc gttcgttcgc cggaattctg cggaacttgc cacaacgagc
    10441 tgaatcccta cggcctctat gtcaagtcca ctcagctcga atggcaggac ggaccctaca
    10501 gccgacaggg cgtccgctgc caggattgcc atatgtggca cgctcccggc acaagcgccg
    10561 acatgggctc gcccgtcgag gatatggccc ggcacacgtt tccgggagcc cacgttccct
    10621 ccaaggtccg gggggcgatc gagatgaaaa tctacgccga cgccgacgag ctcgagccgg
    10681 gcgagaaggc cgtcatttcc ctccatctct acaacgccaa ggccggccac aagatcccga
    10741 ccggctcggt ggaggaccgc cagctctggg tcgaggtcca cgccgtcgac gccgccggga
    10801 tccgctacgc cctgccggtg gacaaaaagg gattcccgga cgaggagtac acgatcacct
    10861 ccaacgcccc ggcttaccaa gatatgggcg acatgatgat ggatctgccc ggatttccgg
    10921 gcgtctcccg ggacgcggtg gccgaggaag gccaccgggt ctttcgcatg ccttatttcg
    10981 atcccaaggg ccggatgacg atcggccaat ggaacaccgc ctccttcgga accgactacc
    11041 ggatcggccc ccgggaaacg aaaatcgaaa cctattcttg gacgcttccc cagactcttc
    11101 ccgaaggccc tctccggatc gaggccaagc tctactaccg gctgctcgtc aaatcggtcg
    11161 ccgatttcct gaaggtcccg cccgaggaat ccgccgccct cgaggtcaat tcggccgaaa
    11221 ccgtcctcca ggtcgtcttt tgaggagccg tcgatgaaaa aaatcttcgc cttcctggtc
    11281 ctcgttgccg gcctcgttca agccgccgcg gccatcccgg ccttcgcccg caagtattcc
    11341 tattcctgcg acgtctgcca tgccccggtg ccccgcctga aggcttttgg ggaggagttc
    11401 atggacaacg gctaccgcct cccggacaac gagccgccgc gggcgacgat cgacacgggg
    11461 gatccgctcc tccttctcca gcgcacgctg cccctggcct tccgtttcga tgcgtacttg
    11521 gcctatgagc cggaagccga catcactgga gatttccggg ctcccttggc gatgaaaatc
    11581 ctcagcggcg gcaacatctc cgacgatatt tcctactaca cgtatttcct gatggccgag
    11641 gacggcaagg tggccggcct ggaagacacc ttcctgagct tccggaacgt cttcggcctg
    11701 cccctggaca tgatgttcgg acaatatcgg gtcagcgacc ccatcaaggc cacggaaacc
    11761 cggctgacgg tggagaatta cgccctcttc aagttccgcg tcggtgacag ccgcatcaac
    11821 ctggcctatg accgaggcct cctgctttcg accaagacgg gattcgggct ggatgtcgtc
    11881 gcccagatcg tcaacggcaa cggcatcgag gagcaggaga ttttcgactc ggacaaatac
    11941 aaaagcgtcg tcggacgcct cgcccagagc ttcgccggag gccgggtccg ggtgggcgtc
    12001 ctgggatacg ccggcaagga agccggggcg ggaggcgcgg tcaatgcggt cacctatctc
    12061 gggccggacc tcctcctgcg cctgccgaac gtcgagatcc tgctcgccta tgtcaagaga
    12121 acggactcca atccgctctt ccagagggag ggccgggagc aaacgagcga ggccgtcctg
    12181 gccgaggcga tcctcagtcc ctggggcgaa cagggacgga cgtttttcac cctggcctgg
    12241 aaccgggtga attcgacctt ggaggcggac gatgccaacg ccctcaccgt gaacgtcagc
    12301 cgcctgatcc gaaggaatct caagtggacg gccgaataca cccgcgactt cgagcggaag
    12361 gaagaccggt ggctgacggg gatcgtcacg gcgttttagc ccccgccggc cggacgacgg
    12421 aatgaacgaa aaaaaacgag gggcccggcc gcgttggatt tcggccgcgg tcctgattcc
    12481 gtctttcctg ggcgcgctgg cctgttctgg ccccccgccg ccggccggct cctcccatcc
    12541 cgccggggcg gccgcgcttt tttcccggga gcgggtcacc atcgccgtga ccgattcggg
    12601 gctcggcggg ctttcgatca tggccgaggc cgcctcccgg atgaaggcgg ctcgggcctt
    12661 ccggggcgtc gacctcgttt tctggaacgc ccttttctcc aacgacagcg gctacaacag
    12721 ccttccgtcc cgggaggaca aggtccggat tttcgacgcc tccttgcgga gcctggccgc
    12781 cgaggtcaag cctgatctca tcttggtcgg ctgcaatacc ctctccgtcc tgctcggcga
    12841 cgtccccttc gcccggaccg cggacgtccc cgtcctcggg atcgtcgaca cgggggtgga
    12901 gatgatggcc gaggcccttt cccggaatcc gcgggcggcc ggccttctct tgggcacggc
    12961 gacgacgatc gccgaggggg agcatcggcg gaagctcctc gcggccggga tcgaggagga
    13021 gcggctggtc tcgcaaccgt gtccggacct cgtcggcttg attgagagcg attggcgggg
    13081 agaggaagcc gcccgcttga tcgagaccta tatcggcgaa gccgcggacc gccttccgga
    13141 cgccggcgcc ccggtttacg cggggctttt ctgcacccat ttcggatacg cctcggccct
    13201 ttgggagcgc gccttcgccc gccgcggcct caccctggcc ggtctcgtca atcccaattc
    13261 ccgctgggtt gatgccctcg atcccccggc ccggagaaac cgcttccccg aaaccgaggt
    13321 tcgggcgcgg gtgatctcga tggtcgaaat cccggccgcc gtccgcgacg ctttggggga
    13381 atggctccgc ccgctttcgt ccgaaaccga ggacgcgttg aaaaattatg atctccggcc
    13441 gggtcttttc gagtggaaat cgcttttgaa gagcgactaa ccgcgccgcc tgaaaaaaaa
    13501 gccggccgga atccgccgcc aagccggctg cggtcttccc cggaccgctg ggatcctcgt
    13561 cccggctgag ttcatcgacc ggcgcggctt cctggtcctt attttcctga agcggattcg
    13621 accgggacct gatccggccg gttccccgtc ccggaccggc gacggctact cgacgacgac
    13681 gccggaggaa tcccgcgtca actcgccgat cacccaagcc cgctggcccc gggcggcgag
    13741 ccagcggatc atccgcccgg cgtcggccgc cttgacgacg gccgtcatcc cgatgcccat
    13801 gttcagcgtc cggtacattt cggcttcggc gacgccgcct cgctcctgga tgacccggaa
    13861 aatgcgcgga acgggccagg agcgcttttg aatccggacg ccgcagccgg cgggaatcac
    13921 ccgggggata ttgtcgtaga atccgccgcc ggtgatgtgg gccatcgcct tgatgtcgat
    13981 cgcggccatg gcttcgagca gcaggcgggc gtaaatccgg gtcgggcgga ggagcgtccg
    14041 gccgaagggg ccggcgagct ccttcttcgt gaacacgcgc cgggcgagcg aatagccgtt
    14101 cgagtggagg ccgctggagg cgagtccgac gacggcgtcg ccgggacggc atttccggcc
    14161 gtcgatgagc cgggacttgt cgaccaggcc gacgcagaat ccggccagat cccactgatc
    14221 ctttccgtag agacccggga gctcggccgt ctcgccgccg agcagggcgc agcccgcctc
    14281 tcggcacccc cggacgatcc ctttcaccac ctgggtgagc atcgccggct tgactttgcc
    14341 gcaagcgatg tagtcgagga aaaacagagg ctccgccccc gtcacgacca catcgtcgac
    14401 gttcatggcc acgaggtcga tcccgacggt gtcgtaccgg ccgaggatgt cggcgatcat
    14461 gatcttggtt ccgacgccgt cggtcgagga aacgagcagc ggctctttca accctttcaa
    14521 ccggggaagg aacaggccgc tgaatccgcc gatccctccg agaacctcgg ggcgcgaggt
    14581 cgtccggacc agcggcttga tccggcggag aaagtcgtcg gccgcggcga tgttcactcc
    14641 ggcccggcga taggtcaggg atttcatggg cgttcctccg gggaaaaatc aggccgttta
    14701 taccacgccc ccggcggccc ggtcaaacca agcccgaaat tccttgacgg gcccgccggc
    14761 cgatttatag aatggagatg cgggagagtc tgatgggtgg aaagagaaag aaggaaaaaa
    14821 tcgtcgtcat cgggggagga acgggctcgt acaacgtcct ttgcggactg aaaaagtatc
    14881 ccgtcgacct cacggccgtc gttacgatga tggattcggg gggaagcacg ggacgcctcc
    14941 gcgatgaatt cggattcctc ccgccgggcg atgtccggcg gtgcctggtc gccctttcaa
    15001 gcgataccgg cctgctccgc aagctcttcg aataccggtt cgaccggggc tcgggtctca
    15061 gcggacacag cttcggcaac ctgttcctga ccgtcctgcg cgacctcacc gggggcgacg
    15121 ccgcggccgt tcgggaggcg gcgaaaatcc tgaatgtccg cggccaggtc ctgcccgtca
    15181 ccctgaacaa ctgccagctc ggtgtcgtcc tggaaaacgg ccagaccgtc gtcggcgaga
    15241 cgaacatcag cattccgcgg cacgatccgc ggctccggat ccgctccctg ttcgtcgttc
    15301 cccgggcccg gatcaacaag gaggccgagg cggccatcct cgaagcggac aaaatcatca
    15361 tcgggcccgg cgacctctac accagcatcc ttccgaacat cgccgtttcc ggggtcaagg
    15421 ccgccctccg gaaaacccgg gcccgcgtca tttatgtctg caacatcatg accaaggcgg
    15481 gggaaacgct cggtttcacg gccgtcgatt tcgtccggga gctggagcat tacctcggcc
    15541 ggggaatcgt ccaggacatc gtctgcaacg accaaaagcc gcccgcccgg atgttgaaac
    15601 gatacgcggc cgagagggct gaattcgtcg agccggatct cgaggaaggc cgcgtccgcc
    15661 gggcttccct gctgggcggc cgcgacctag cccgacatga ttccgaccgt ctcgcccggg
    15721 tcctgatgag tctctgacct ccccggttct ccgggcgggc tccctaccgg gggcttcgga
    15781 tcttcaccgt gaggatctga aagggcttga gatcgacata aatcgaccgg gggacgacct
    15841 caaaggcatt ggccggatgc tcgccgatgt ccgtcgccgt atacgtcagc gtttcggccg
    15901 tgaacaggct tgaggacacc cgggtcctcg tctcccgccc ggcgatttcc ctgagccgga
    15961 gaacgatccc ctcgcccgat tcgggggccg tcaccgtctg gatcaggacg ttcggctgat
    16021 cgacggcgaa aaagctcagg gaatccgcgg gaaggacgcc ggccgtttga ttcggcagcc
    16081 attccgcgag aagcggcgtc cgggcttccg ccccgaaccg ggccgccgcg acctgatcgg
    16141 agccgcccgg ccggctggtg agggcgtagc ggaaaacgag cgggccgccc tggctggcct
    16201 taaaattcgt catccagtag ttgttgagga cataggaaaa aacgaagggg cgggcgacat
    16261 ccatcttggc ttgccatttc ccggtgttga tgtcgccgag ctggacgagg ggggcttcca
    16321 gcggagccca gaccaccgtg tttttcgtcc cggccgcctc gatccagttc tggacggcca
    16381 gccagtcgcg ggtcgttccc ggaagcggat cggcttcggg agacatcacg gcgtccgcga
    16441 tttcgagccg gagccggccg ttctccaccc ggaagggaaa ggcgaaatac gcggcctcgg
    16501 cttcggtcgt ctcctccttg cgcatccggt tgatgaaatc gatccgcttg agatcatcgt
    16561 aaaggatgat ttcctgccgg atctccggaa accggaacat ccgggaggaa aggacaaggc
    16621 tcgaggcgac cggcccgcgg aagccggcgg acacggcggc ggattccggg aaataccgcc
    16681 ggaaggcggc ccgcttggac cggtcatcga cggcttcccg gccgccttcc gggttctcgt
    16741 aaatcagctg gttaagcccg aaggcggaag ccggatcgac cagctcccgg cccgtctcct
    16801 tgtcgacgag gcttcgcagc ccgccggtga ccggatcggc cgtcacctgg aaaaatcggt
    16861 tttcgatccg gttgtcctcg atcaccgctc ccgcttcggc cggcgcggaa ggctctccgg
    16921 gagcagccgt gaagacggca tagccgatcg aggggacgtt ctccgcccgg aacatcaaga
    16981 tgttc
//
LOCUS       DAOOXH010000052        16661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_3385_length_16661_cov_8.930627, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000052 DAOOXH010000000
VERSION     DAOOXH010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 16661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16661
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3385_length_16661_cov_8.930627"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..470)
                     /locus_tag="PKV64_07260"
     CDS             complement(36..470)
                     /locus_tag="PKV64_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16912.1"
                     /translation="MEYRATRILPIAFLGFILAAAAAFGSPVIKLKVTAEIANVRVKP
                     FIGSNIVRQFPEGTVLEAERKEGEWFLVKMEPDETGTISGYVHESLVLALEDIPITEP
                     VPAPVVEIPPVEEVEKEEPPPAKAVPRPKATQPAPVEPPPRP"
     gene            complement(529..1425)
                     /locus_tag="PKV64_07265"
     CDS             complement(529..1425)
                     /locus_tag="PKV64_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16913.1"
                     /translation="MKRFLFPGVVLALAVLAAGPAASFGRESAPSTAGDAPPRLWERV
                     EIVVFGGLGLPAAEGMSLHRAETASPAASPTVENRIRTSSAPAVFTGAAATFFLKNGF
                     GIQAGFGYLKAGLESVGRFSWNPPGSTPASTAVDGGGEGEITSVPFFIGVFKSFEFRF
                     AGKALRAHVAAGPALFFHSILTSSQAGAAAIRDGLTDAILVPVRVEDTTWISPGATAG
                     AGLDLPLSPALSLAVEGRYGFAPRKEFSWIWTPGVYDGIFHRVAAFNFDEAAAAREAG
                     RTTPLSVDPSFFQIAIGLKLIF"
     gene            1631..2401
                     /locus_tag="PKV64_07270"
     CDS             1631..2401
                     /locus_tag="PKV64_07270"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPN16914.1"
                     /translation="MSRNRSLKTAVALCLAAVLAAGAAAAVKPKIVFKTESWDFGKIK
                     QGQDVSVDFVFTNEGDDVLQIGNVESSCGCTAVLVSDKKVNPGKAGKLQVTFKSAGYS
                     GEVVKYIYVESNDPNARRIQLKVQAAVDVPPQPKIDLSQYTYDAGLIVEGESLETPVV
                     VKNRGELELSFECALPGAAFFAKGKPAAFPIKVAAGKEAEITVRFPLAGRAGFVREFI
                     LFKTNDPLRSTISMTLSGYVVTKLQLKQIFEKYKTIIK"
     gene            2472..3596
                     /gene="dprA"
                     /locus_tag="PKV64_07275"
     CDS             2472..3596
                     /gene="dprA"
                     /locus_tag="PKV64_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPN16915.1"
                     /translation="MPLFKGRRSPEKIAFLALNLALWDRLAAVKKILTLFPDPAGAFA
                     LSSEEWSTIGFRPQIAARLGAPGLLDRAEKEFDRSKEKGYSLLTLGDAEYPSFLREIF
                     DPPAVLYCAGRTDILERPAVALVGSRRPTPYGRNVAERLARDLSGRGIVVVSGLAVGI
                     DAAGHQGALDGGQTVAVLGSGLDVPYPRANRRLFERITEEGAVISEFPLGTDPLAANF
                     PRRNRIISGLSRALIVVEAAEKSGSLISAGFALEQGREVMAVPGNITSEVSRGTNGLI
                     KAGAKLVETWKDVAEELPAPVREALLSREEDEPREPLPLLSSGEAAVRRLLSPESPVH
                     VDELLERTELSISELLTILLSLEIKGVAAQLPGKRYLRRL"
     gene            3596..5929
                     /gene="topA"
                     /locus_tag="PKV64_07280"
     CDS             3596..5929
                     /gene="topA"
                     /locus_tag="PKV64_07280"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPN16916.1"
                     /translation="MDKALLIVESPAKAKTINKYLGSGYRVMASMGHVRDLPKSKLGV
                     DVDKDFEPQYIIIPEREKVVGELRAAARTASSIILAADPDREGEAICWHLKAIFDDGK
                     KPVFRVRLQEITKQAVEDAVRHMGELNVHMFEAQQARRVLDRLVGFKISPLLWKKIGR
                     GLSAGRVQSIALRLVVEREREIRAFRKEEFWTIFAYLEASLPPRFKAALSKIDGKKAK
                     IPDGASAQAIVDALKGARYVVRDVAVTEKKRNPPPPYITSTLQQEAFRLLRFPVKKTM
                     SVAQKLYEGLPLGERGPMGLITYMRTDSVRISAEAQAWARAAIVQSFSAAHVPSHPRV
                     HANKGKAQDAHEAIRPTSPELTPEAVRPYLKKEEFSLYQLIWNRFFASQMSAAVLEET
                     ALTIAAGERFLFTVKGGVIKFKGWWELYPKKEEDEEDALPPVKKDETLTLAELEPKQS
                     FTQPPARYTEGTLVKELEAKGIGRPSTYAPIIATLQNRVYVVKEENKFVPTDLGMFVT
                     DFLVKHFPDLMEYKFTARMEGELDEVSEGTLDWVGAMRDFYGRLQNDLAAAGKVESVA
                     GTGIPLDEKCPKCGKPLVNKGGKFGRFKSCSGYPECDYSDDLYKKSEPVPLEEKCPTC
                     GANLVQRVGRYGMFVACSNYPKCRFIKGKEKVDTGIACPQGCGGTLLKRKTKREKFFY
                     GCSSFPKCRFATWDEPLPESCPKCGRPFVLRKTTKKDGTYKHCVDENCGWREPASPPA
                     VPPRPAGEEPEPAGKDGDSREDRGGAPDKPGPEAGGV"
     gene            6095..6979
                     /gene="xerC"
                     /locus_tag="PKV64_07285"
     CDS             6095..6979
                     /gene="xerC"
                     /locus_tag="PKV64_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HPN16917.1"
                     /translation="MRKNIDEFLEYLRYERNASAHTVAAYRRDLLQMAAYLDRRKVRL
                     ARVDNIILRGWMADLHDRRLSKTSVARKLAAVRSFFQYCLKRRICDDNPAVVVTRPKL
                     DSPVPGFLSEGEIERFLDLPDRNDRLGPRDRAIFELFYATGIRVGELAGLDVSDVGLE
                     ERLLRVRGKGKKERIVPFGRTAASSLAAYFETRPGFLKGPLDQAALFLNYRGGRITSR
                     SVERLVAKYIRKTAIRRKISPHSLRHSFASHLLGRGADLRVIQELLGHESLGTTQKYT
                     HLDLPQLLAVYRKSHPRA"
     gene            7139..7441
                     /locus_tag="PKV64_07290"
     CDS             7139..7441
                     /locus_tag="PKV64_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPN16918.1"
                     /translation="MKSLWMKGAVLGIIAALLVVPALAQQAAAAGGEKAAGGKINLNT
                     APLDELQKLPGVGAAIAQRIIDFRKMNGPFKKIEDLMKVRGIGEKLFLRFKDQLSV"
     gene            complement(7515..8135)
                     /gene="rdgB"
                     /locus_tag="PKV64_07295"
     CDS             complement(7515..8135)
                     /gene="rdgB"
                     /locus_tag="PKV64_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPN16919.1"
                     /translation="MTSAAGPGEPLLIATTNRGKAREIAAALAMQAVRFVALDDAGIE
                     AVCPETEKTLAANARAKAEFYSRRSGLLTLAEDSGLEIKVLGGEPGVRSARFSGPKAT
                     DAKNVSKVLRLLASVPRSGRRARFVCVMALARNGRIVAEARGVVRGTIAVGPKGTAGF
                     GYDPIFYYHPRGKTFAELSPEEKNAVSHRGRALAAMKIRLRRFLSK"
     gene            complement(8132..9040)
                     /locus_tag="PKV64_07300"
     CDS             complement(8132..9040)
                     /locus_tag="PKV64_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HPN16920.1"
                     /translation="MNAYLRALRLERWPRSSAIFLGSAVFVFLHREDVAALSPAETIW
                     RLAAAFLLTWGISTANYVLNEIVDRPFDVHHPTKRLRPLVSGEIKAFPFALIGLLLVA
                     ACGGAAVLLFDRTFTLALLSLLAAGIVYNVPPIRTKDIPFLDSVSESVNNPIRFLIGW
                     SALAVSGKLPPLSVLVCWWAFGNFLMVAKRLSEFRFLKEKAGEYRISHRKYSRTSLVI
                     GMSVSALICLLAFLYFGWIFKLQSFYAIAPFLLVFFGLIFRKTLREAEMMEEPEKLFI
                     HPKYALYALFLVLLFVLAFLKDAIGQ"
     gene            complement(9037..9945)
                     /locus_tag="PKV64_07305"
     CDS             complement(9037..9945)
                     /locus_tag="PKV64_07305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN16921.1"
                     /translation="MTGRFLRAGACPVCRGLDIRPWKKGTFDFSGLRSDQIKITDSEY
                     GKTWDLQACGACGHIFADPYPAPDFLASLYGEVEDPLYDQEAEGRTRNFLPILNRLDL
                     FRPGRGALFDVGAATGILGDAARRRGWRVDGVEPSAWAVRKAAEKYGLTLRRGGFEDI
                     AAAGDSFQAVTMVDFIEHTPRPSEAAAMAFSLLAPGGVLCLVTPDIHSPAARLAGRRW
                     WHLRPGHLAYFSKRSLRVLLEGAGFRIVEIRRYAWTFSLHYLVSRFRWMDSFARSRAS
                     SFLRKIPIKLALGDSFEIYAVKDPFS"
     gene            complement(9942..10328)
                     /locus_tag="PKV64_07310"
     CDS             complement(9942..10328)
                     /locus_tag="PKV64_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN16922.1"
                     /translation="MTETKRRILIIDDERSVVQSIALALAGEGVEIETVLSGEDALIR
                     TAAVRYDLIICDLMMPGLSGLDLLKSLRTYRPTTPVLMITGYPTIKTAQESLKMGAFA
                     FITKPFTPAEIRSAAARALAQADSKE"
     gene            complement(10325..12235)
                     /locus_tag="PKV64_07315"
     CDS             complement(10325..12235)
                     /locus_tag="PKV64_07315"
                     /inference="COORDINATES: protein motif:HMM:NF028511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPN16923.1"
                     /translation="MILAILAIVLFGGVVFGILGLRLEHQTIMSLAENRIYHDMDSAR
                     TIYSFYLDNLLTAVTLTAESELLRLPRSAQPNAELDFHLARAAEAGRFDFLSFAAADG
                     RRIALSDPAVPAGGFRDRDSFFSRVLEGKRLAATRLLPLDSLRRESTRFEESAAGDIL
                     VLVAAAPVRGPEGRIIGVLEGGRRINGDNEIVDRVKAAIFDPEKRGGRDTGVVTIHQG
                     DIRASTTLKNPQGSRLNGTRADPRFRQEALERRKAWAGRAVILGERYISASEPLADYD
                     GTVVGMLSIGLLERPFLALRNRVITQNAAWAAATAAVLLILLSLMATRLVRPLGDLAA
                     AASRIAQGDLNLKVPVGGRDEIGELGGAFNRMTAELRKAKENLLQWTWTLESRVADRT
                     HALRNIQESMARSEKMASLGQLAAGIAHEINNPLTTILINAHLLLEKARPDDPAAAAL
                     RLIAEETERCGRIVKGLLEYSRRTPPEKSALDVNAVVERTIGLLSGQAWARNVRLSHH
                     LDRTLPPLFLDRAKIQQVFWNLILNACEAMPSGGTIDVSSRRSADGRSIEVVFADSGP
                     GIPEENLDRIFDPFFTTKSSGTGLGLAVAASLVEQHGGTISARNGAGRGAVFTIQWPL
                     AETETGDRGIGP"
     gene            12378..12845
                     /locus_tag="PKV64_07320"
     CDS             12378..12845
                     /locus_tag="PKV64_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPN16924.1"
                     /translation="MRLSTKGEYASRAMLELTLHYGGPPLHIRDISRVQDIPERFLEQ
                     ILLQIKRAGYLRSRKGPNGGYMLSKPPEEISVAEIIRVMDGPLAPIDCVSVTAREVCP
                     REKSCGLRWLWKRNRDAIAEILESTSFADVAEMTRKAMRANNENGNQKTPDKG"
     gene            12909..13892
                     /locus_tag="PKV64_07325"
     CDS             12909..13892
                     /locus_tag="PKV64_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HPN16925.1"
                     /translation="MSKWKKTAGLLIAVLALIPAAFAEEKTPPPSVTAGKTLKLSGFT
                     QVLGALQDQGVDALSVRRSRFSLSADLMKNIKAKFTVDAVRNPILVDAMVDVVFAESA
                     ALRFGQFRVPFGAENDTSSADLDTINFSQPVSKLAPGRDVGTMGRDVGAMLTGQVSVF
                     DYSLGLFNGAGANKTDTNDAKDFAGRVGVKPFDFLTIGASLYKGKADAAAGAPLIRRD
                     RTGLHAGVSFGDVSLKGEMIWAADGEMDRRGWFVQAGWFVLPKQLQIVAKVDAYDKNT
                     ALDDDRTNLYTAGLNWMISGKTKLQVNYEYLRNELGGTVNKALLVQFQAGF"
     gene            13911..14861
                     /locus_tag="PKV64_07330"
     CDS             13911..14861
                     /locus_tag="PKV64_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HPN16926.1"
                     /translation="MKKLMIAVLLAVSVLGGFAAGPAQAPVLEGQVSISGAWALYPMV
                     VKWAEEFQILHPRVRIDVQAGGAGKGIADVIADMVDFGMVSRDVHPVEMEKGALPFAV
                     TKDAVVPMINSKNPYLPEILRKGLTRQAFIDIWITKSATVWGDVLDGKAPAPIHVFTR
                     SDACGAAETWALFLDKRQEDLGGVAVYGDPGLADAVRRDPLGIGFNNVNFAYDPKTLA
                     PIAGLVAVPIDLDGNGMIDPVERFYGTRDDLTKAIGENKYPSPPARDLYLVSKGVPAK
                     PAAAAFLRWILGEGQKYVPETGYIRLSEDKLKAGLALLDK"
     gene            14921..15808
                     /gene="pstC"
                     /locus_tag="PKV64_07335"
     CDS             14921..15808
                     /gene="pstC"
                     /locus_tag="PKV64_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPN16927.1"
                     /translation="MVKRLFKELAARRLMFLVTILPGALVFLMLFGLYSRSRSILALH
                     SFSDLLFGSSWFPLKGEFGLYPFLMGTVWVTLAAMAIAVPVSLLSAIYLSEYAPRRIR
                     ETIKPIVDLLSGIPSVVFGVWGVLMIVPFVAKIAPAFGAYSTGYSVLAGAIVLAIMVF
                     PTIIHVSLEVFASVPQSLRDASLSLGATKWETVKHVVLRKGLAGIIASVALGFSRAFG
                     ETMAVLMVVGNTPIIPKSVFDSAYPLPALLANNYGEMMSIPLYDGALMLAAFVLLLIV
                     LVFNIGARVVLIRAEQKAE"
     gene            15805..>16661
                     /gene="pstA"
                     /locus_tag="PKV64_07340"
     CDS             15805..>16661
                     /gene="pstA"
                     /locus_tag="PKV64_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPN16928.1"
                     /translation="MMGFRRRKLEESIFKVLMIGSTVIVIGSLLLIIATVIVKGFPAM
                     NLAMITQTPKGGYYLGKEGGILNAIVGSLELAGGATLLALLFSLPIVLYINLYKRRKS
                     RFAVFVRFSLDVLWGIPSIVYGAFGFILMMMVGLRTSLLAGIIAVALLELPIMARAMD
                     EVVRLVPSSLQDASASLGATKLETAVHVIVRQCLPGIITAILIAFGRGIGDAASVLYT
                     AGFSDRVPGALLQPAATLPLAIFFQLGTPYPEVQERAYASAAILTIIILVISLLSRWY
                     SRKFRKNSA"
BASE COUNT         3225 a         4981 c         5328 g         3127 t
ORIGIN      
        1 tcccccgccg tccgtctacg aggcgacccg ggaaatcatg gccgcggcgg gggctcgacc
       61 ggggcgggtt gggtcgcttt cggccggggg acggccttgg ccggcggcgg ctcttccttt
      121 tcgacttcct cgaccggagg gatctcgacg accggagcgg gaacgggctc ggtgatggga
      181 atgtcctcga gagccaaaac caggctttcg tgaacataac cggagatcgt gccggtctcg
      241 tcgggctcca tcttgaccag gaaccattcc ccttccttgc gctcggcctc gagaaccgtg
      301 ccttcgggga actggcggac gatgttgctg ccgataaacg gcttgacccg gacgttggcg
      361 atttcggccg tgactttgag cttgatgaca ggcgagccga aggcggctgc cgcggcgagg
      421 atgaatccca ggaaagcgat cgggaggatg cgggtcgcgc gatattccat ttccaacctc
      481 cgatccggat tcgccttcag tatagcacgt ttgcggccgg gcggcggctt aaaaaatcag
      541 cttcaatccg atggcgatct gaaaaaacga cgggtcgacg gacaagggtg tcgtccgccc
      601 ggcttcccgg gccgcggccg cctcgtcgaa gttgaaagcg gccacccggt gaaaaatccc
      661 gtcatagacg cccggcgtcc aaatccagga aaattctttc cgcggagcga agccgtaacg
      721 tccctcgacg gcgagggaaa gggccggaga gagcggaaga tcgagtccgg ccccggccgt
      781 cgcgccgggg gagatccagg tggtgtcctc cacgcggacg gggacgagga tcgcgtccgt
      841 caggccgtcc cggatcgccg ccgcgcccgc ctggctcgag gtcaggatcg aatggaaaaa
      901 aagagccgga ccggcggcga cgtgcgcccg aagagctttt ccggcgaagc ggaattcgaa
      961 gctcttgaaa accccgatga aaaaggggac ggacgtgatc tcgccttcgc cccctccgtc
     1021 gacggcggtc gaggccggcg tcgatccggg cggattccag gagaaccggc cgacgctttc
     1081 caatccggct ttgaggtagc cgaagcccgc ttggatcccg aatccgtttt tcaagaaaaa
     1141 ggtcgcggcc gcgccggtga acacggccgg ggcggaggat gtccggattc ggttctcaac
     1201 cgtcggggaa gccgccggag aggcggtctc ggcccgatgg agcgacattc cctccgccgc
     1261 cgggaggccg agtccgccga aaacaacgat ttccacccgt tcccagagac gggggggagc
     1321 atcgccggcc gtggacgggg cggattcccg gccgaaggag gccgccgggc cggcggccag
     1381 gaccgccagg gccagaacaa cgcctggaaa gagaaaccgt ttcattttgt catccgttcg
     1441 aacctcaaag ggttggcggc agccatgagg acgcctatct tacttgccgc ggcgttccgc
     1501 ggcaacctcc cgatgaaggg acgcctcatt catcccctcc cttccctgga gggacttcgt
     1561 gaggcgttcg cttgcctcct tgaagagggg ggttgaaatg atgtacattt tggtcgtcag
     1621 ggaggttccc atgagcagaa accgttcgct taaaacggcc gtcgcccttt gcctcgcggc
     1681 cgttctggcc gccggagcgg ccgcggccgt gaagccgaaa atcgtgttca agacggaatc
     1741 gtgggatttc ggcaagatca agcaaggtca ggacgtctcg gttgatttcg ttttcacgaa
     1801 cgagggcgac gacgtgctcc agatcggcaa cgtcgagtcc tcctgcggct gcaccgcggt
     1861 cttggtctcg gacaaaaaag taaatcccgg caaagcgggg aagcttcagg tcacgttcaa
     1921 atccgccggc tacagcggcg aagtcgtcaa atatatctat gtggagtcca acgacccgaa
     1981 cgcccgccgg atccagctta aagtccaagc ggccgtggat gtcccccctc agccgaagat
     2041 cgacttgagc cagtatactt atgacgccgg gctgatcgtt gaaggggaat ccctggagac
     2101 tcccgtcgtc gtcaagaacc gcggcgagct ggagctctcg tttgaatgcg ccctgccggg
     2161 agccgccttt ttcgccaagg gaaagccggc cgcctttccc atcaaggtcg cggctggaaa
     2221 agaagcggag atcaccgtcc gtttcccgct ggcgggccgg gcgggattcg tccgtgaatt
     2281 cattcttttt aagaccaacg accccctccg gtcgacgatt tcgatgactc tcagcggata
     2341 cgtcgtgacc aagcttcagc tgaagcagat tttcgaaaaa tacaaaacca tcattaagta
     2401 gcccgcgtcg gactccgagg cggttcccgg gccctgagaa aaatccggtt ccgggccgtc
     2461 ctttgatggt gatgccgctc ttcaaaggac gccgttctcc ggagaaaatc gctttcttgg
     2521 ccctcaacct ggctctttgg gaccggttgg cggccgtgaa aaaaatcctg accctgtttc
     2581 ccgacccggc cggagcgttc gctctctcct cggaggagtg gtcgaccatc ggtttcaggc
     2641 cgcagatcgc ggcccggctc ggcgcgcccg ggctcctgga ccgggccgaa aaggaatttg
     2701 acaggtccaa ggagaaagga tattctctcc tgactctcgg ggacgccgaa tatccctcct
     2761 ttcttagaga gatattcgat ccgccggccg tcctgtattg cgcgggacgg accgacatcc
     2821 tcgagaggcc ggcggtcgcc ctggtcggtt cgcggcggcc gacgccgtac ggacggaacg
     2881 tggccgaacg tttggcccgc gacctgtccg gacgggggat cgtggtggtc agcggcttgg
     2941 cggtcggcat cgacgccgcc ggacaccagg gcgccctgga cggaggacaa accgtggccg
     3001 ttctgggatc cggtctcgac gtgccttatc cgcgggccaa ccgccggttg ttcgagcgga
     3061 tcaccgagga gggggctgtg atatccgagt ttcctctggg aacggacccg ctggcggcga
     3121 atttcccccg gcgcaaccgg atcatcagcg ggctttccag ggccctgatc gtggtcgagg
     3181 cggcggaaaa gagcggttcg ttgatttcgg ccggtttcgc cctcgagcag ggccgggagg
     3241 ttatggccgt gccgggcaac atcacttccg aggtcagccg cggaacgaac ggcctgatca
     3301 aggccggggc gaagctagtc gagacctgga aggatgtggc cgaagagctg ccggccccgg
     3361 tccgggaggc gttgctgagc cgggaagagg acgaaccacg ggagcctctg ccgctgcttt
     3421 ccagcgggga agcggccgtc cggaggcttc tttcgcccga gtccccggtt cacgtggatg
     3481 aattgctgga acggacggag ctctcgattt cggagctcct gaccatattg ctttccttgg
     3541 agatcaaggg cgtcgccgcc cagctccccg ggaagcgcta tttaaggaga ctgtgatgga
     3601 caaggccctc ttgatcgtcg agtcgccggc caaggccaag acgatcaata aatacctggg
     3661 ttcgggctat cgggtgatgg cctcgatggg gcacgtccgg gatcttccga aaagcaagct
     3721 cggcgtggac gtcgacaagg atttcgaacc ccaatacatc atcattcccg agcgcgagaa
     3781 ggtcgtcggc gagcttcggg cggcggcccg gacggcctca tcgatcatcc tggcggccga
     3841 tccggatcgc gagggagaag cgatctgctg gcatctaaaa gcgattttcg atgacgggaa
     3901 gaagccggtg ttccgcgtcc ggctccagga aatcacgaag caggcggtgg aggacgccgt
     3961 ccggcacatg ggcgaattaa atgttcatat gttcgaagcc cagcaggccc gccgcgttct
     4021 cgaccggctt gtcggcttca agatcagccc gctgctttgg aaaaagatcg gccggggctt
     4081 aagcgccggg cgcgtccaga gcatcgccct tcgcctcgtc gtcgagaggg agcgcgagat
     4141 ccgggctttc cgaaaagagg aattctggac gatcttcgcc tacctggagg cctcgctccc
     4201 cccccggttc aaggcggcgt tgtccaaaat cgacggaaag aaagccaaaa tccccgacgg
     4261 cgcctcggct caggcgatcg tcgatgcgct gaaaggcgcc cgctatgtcg tccgagacgt
     4321 cgccgtcacc gaaaagaaga ggaatccgcc gccgccctat atcaccagca ctcttcagca
     4381 ggaagctttc cggcttctcc gcttccccgt caagaagacg atgtccgtcg cccagaagct
     4441 ctacgagggg cttccgctgg gtgaacgggg gccgatgggc ctcatcacct acatgcgcac
     4501 tgattcagtc cggatttcgg ccgaggccca ggcgtgggcc cgggcggcca tcgtccagtc
     4561 cttttcggcg gctcacgttc cctctcatcc gcgggttcac gccaacaaag gcaaggccca
     4621 ggacgctcac gaggccatcc ggccgacttc gcccgaactg acgccggagg cggtccggcc
     4681 ctatttgaaa aaagaggaat tcagcctcta ccagctgatt tggaaccggt tcttcgcctc
     4741 ccagatgagc gcggcggttc tcgaagagac ggccctgacc atcgccgccg gcgagcggtt
     4801 cctgtttacg gttaagggcg gcgtgatcaa gtttaagggc tggtgggagc tttatcccaa
     4861 aaaagaggag gacgaggaag atgccctccc gccggtcaag aaagacgaaa cgctgaccct
     4921 ggccgagctt gagccgaaac agagcttcac ccagcctccg gcccgttata ccgagggaac
     4981 gctcgtcaag gaacttgaag ccaagggaat cggccggccc tcgacgtacg ccccgatcat
     5041 cgccaccctg cagaaccgcg tgtatgtcgt caaggaggag aacaagttcg ttccgaccga
     5101 tctcggcatg ttcgtcaccg atttcctggt caagcatttc cccgacctga tggagtacaa
     5161 attcacggcc cggatggaag gggagctgga tgaagtgagc gaggggacgc tggactgggt
     5221 cggggcgatg cgggatttct acggccggct ccaaaatgat ctcgcggccg cgggcaaagt
     5281 cgaaagcgtc gccgggacag gaatccccct ggacgaaaag tgcccgaaat gcgggaagcc
     5341 gctggtcaac aagggcggca agttcggacg gttcaagtcc tgctcgggat atcccgaatg
     5401 cgattactcg gatgatcttt ataaaaaaag cgagcccgtt cccctcgagg aaaaatgccc
     5461 gacgtgcggg gcgaacctgg tccagcgggt cggccgctac gggatgttcg tggcctgctc
     5521 gaactatccg aaatgccggt tcatcaaggg gaaggaaaag gtcgatacgg ggatcgcctg
     5581 tccccaaggg tgcggcggga cgcttttgaa aagaaagacg aaacgggaga aattctttta
     5641 cggctgcagc tccttcccga agtgccggtt cgccacctgg gacgagccgc ttccggaatc
     5701 ctgtccgaaa tgcggccggc cgttcgtcct ccggaaaacg accaagaagg acgggacgta
     5761 caagcactgt gtcgacgaga actgcggctg gcgcgagccg gcgtctcctc cggccgtccc
     5821 gcctcgtccg gcgggggaag aacccgagcc ggccgggaaa gacggcgact cgagggaaga
     5881 cagggggggt gctccggaca aaccggggcc ggaagccggc ggggtctaag atcaattccc
     5941 aaaatcgcgg ccgtggcgca gcttctccgc cttgagggag aacgcctgag gagtctggga
     6001 agaataaacc ggaaatatcg atccgccttt tttttggata tgcggacgga tttcgttaaa
     6061 atgaaacagg gccggcggcg ccggatccgg acgcatgcgc aaaaacatcg atgagttcct
     6121 cgagtacctc cggtatgaaa gaaacgcctc ggcccatacc gtggccgcct accggcgcga
     6181 tcttctccag atggccgctt atcttgaccg ccgaaaagtc cgcctggccc gggtcgataa
     6241 catcattttg cgcggctgga tggcggatct tcacgaccgg cgcctttcca agacgtccgt
     6301 cgcccgcaag ctggccgccg tccggtcgtt tttccagtat tgcctcaagc gacggatttg
     6361 cgatgataat ccggccgtgg tcgtgacccg gccgaaactc gacagtcccg tccccggttt
     6421 tctttcggag ggggagatcg agcggtttct ggatcttccg gatagaaacg accgtctggg
     6481 gccgcgggac cgggccattt tcgagttgtt ttacgccacc ggcatccggg tcggcgagct
     6541 ggccggcttg gacgtctccg acgtcggctt ggaggagcgc ctcctccgcg tccggggcaa
     6601 ggggaaaaag gagcgcatcg ttccgttcgg gcggacggcg gcctccagcc tggcggctta
     6661 ttttgaaaca aggcccggct ttttgaaggg acctctcgac caagcggcct tgttcctgaa
     6721 ttaccggggg ggacggatca cgtcccgttc ggtggagcgg ctggtcgcca aatatatcag
     6781 aaaaacggcc atccggagaa aaatcagccc ccattccctt cggcattcgt tcgcttccca
     6841 tctcctcggc cggggggccg atcttcgggt catccaggag ctgctcggcc acgaaagcct
     6901 ggggacgacc cagaaataca cccatctgga cctgccccag ttgctcgccg tctaccgcaa
     6961 gagccatccg cgggcttgac gtcccgtctc ccgccgttga cgcccgaatc gccatccgtc
     7021 tccaaaaatt gccgggggcg gagccgcggt tcgattcaag tcattgaaat aaatgaagat
     7081 aataaaattt cggttttggc atgccgctcg ctaaagaaag ggcgaaagga ggctcaacat
     7141 gaagagcttg tggatgaaag gggcggtttt gggaatcatc gccgctctct tggtcgttcc
     7201 ggccttggcc caacaggcgg ccgccgccgg cggcgaaaag gccgcgggcg gaaagatcaa
     7261 tttgaacacg gcgccgctgg acgagctgca aaagctcccc ggcgtcggcg ccgcgatcgc
     7321 tcagcggatc atcgatttcc gcaagatgaa cggtccgttc aagaagatcg aagacttgat
     7381 gaaagtccga ggaatcggcg agaagctttt tctccgattc aaggaccagt tgagcgttta
     7441 agatccggcg ctccctccac gcgtgcggga atatcccgcc ggcggccctt cccggaaggg
     7501 gaggaccgcc ttttttattt cgagagaaag cgacggagac ggatcttcat ggcggccagg
     7561 gcccggccgc gatggctgac cgcgtttttc tcttcgggag agagctctgc gaatgtcttg
     7621 ccccggggat ggtagtagaa aataggatcg tatccaaagc cggcggttcc cttgggtccg
     7681 acggcgattg ttccgcggac gacgccgcgg gcctcggcga cgatccggcc gtttcgggcc
     7741 agagccatga cgcagacgaa gcgggcccgc cggccggacc ggggaacgga ggcaaggagg
     7801 cggagaacct tggagacgtt ctttgcgtcc gtcgccttcg gcccggagaa ccgggcggag
     7861 cggacgcccg gttctcctcc gaggaccttg atttccaggc cggaatcctc ggccagggtc
     7921 aggaggccgc ttcggcggct gtagaattcg gctttggccc gagcgttggc cgcgagcgtt
     7981 ttctctgttt cggggcagac ggcttcgatg cccgcgtcgt ccagggcgac gaagcggacg
     8041 gcttgcatcg ccagggcagc ggcgatctcc cgggctttcc cgcggttggt cgtcgcgatc
     8101 aggaggggct ctcccgggcc ggccgcggat gtcattgtcc gatggcgtcc tttaagaacg
     8161 cgaggacgaa cagaagaaca agaaagagcg catagagcgc gtatttcgga tggatgaaaa
     8221 gcttttccgg ctcctccatc atctccgcct cccggagcgt tttccggaaa atcaagccga
     8281 agaagacgag gaggaacgga gcgatggcat aaaaggattg aagcttgaag atccagccga
     8341 agtaaagaaa ggccagaaga cagatcaggg cgctgacgga catgccgatc accagcgacg
     8401 tccgcgagta tttccggtgg gatatccggt attctccggc tttctccttg aggaaacgga
     8461 actcggacag ccgcttggcg accatgagaa aattgccgaa cgcccaccag cagacgagga
     8521 cggagagggg aggaagcttt ccggaaacag ccagggccga ccagccgatg agaaaacgaa
     8581 tcggattatt gaccgattcg gagacggagt cgaggaaagg gatgtccttg gtccggatgg
     8641 gggggacgtt gtaaacaatc ccggcggcga gcagggacag cagggcgagg gtgaatgtcc
     8701 ggtcgaacaa gaggacggcc gccccgccgc aggcggcgac gagaagaagg ccgatgagag
     8761 cgaagggaaa ggctttaatt tcaccgctga caagaggccg gagacgcttg gtcggatggt
     8821 ggacgtcgaa ggggcggtcg acgatttcgt tgaggacgta attggccgtc gagatgcccc
     8881 aagtcagaag gaacgccgcg gccagcctcc agatggtttc cgccggagag agcgcggcga
     8941 catcctcccg gtgaaggaaa acgaagacgg cgctgcccag gaaaatggcc gagcttctcg
     9001 gccaccgctc caggcgcagg gcccgcagat aggcgttcac gaaaatggat ccttgacggc
     9061 gtatatctcg aacgaatcgc cgagggccaa ttttatcggg atcttgcgga gaaatgaaga
     9121 ggcccggctt cgggcgaagg aatccatcca gcggaagcgg gagacgagat aatgaaggct
     9181 gaatgtccag gcatagcggc ggatttcgac gatccggaat cccgctccct caagaagaac
     9241 ccggaggctt ctcttggaaa aataagccag gtggcccggc cgcaggtgcc accagcgccg
     9301 gccggccagg cgggccgccg ggctgtgaat gtcaggggtg acgaggcaca ggacgccgcc
     9361 cggggccaga agggagaagg ccatggcggc cgcctcggac gggcggggcg tgtgctcgat
     9421 gaaatcgacc atggtcacgg cctggaagga atcgccggcg gcggcgatgt cctcgaatcc
     9481 tccccggcgg agggtcaagc catacttttc cgcggccttc cggacggccc aggcgctcgg
     9541 ctccacgccg tcgacccgcc agccgcgccg gcgggcggcg tcgcccagga tgcccgtggc
     9601 cgcgccgacg tcgaacaggg ctccgcggcc gggacggaag agatccagcc ggttcaggat
     9661 gggcaggaaa ttccgcgtcc gtccttcggc ctcctggtca taaagcggat cttccacttc
     9721 cccgtaaagg gaggcaagga aatccggcgc cgggtacgga tcggcgaaga tgtggccgca
     9781 ggcgccgcag gcttgaaggt cccacgtttt cccgtattcg ctgtccgtga ttttaatctg
     9841 gtcggatcgg aggccggaga aatcgaacgt cccttttttc catggacgaa tgtcgaggcc
     9901 gcggcaaacg gggcaagccc cggcgcgaag gaaacggccg gtcattcctt cgaatccgct
     9961 tgggcgaggg cccgggccgc ggcgctccgg atttccgccg gcgtgaacgg cttggtgatg
    10021 aaggcgaagg cgcccatttt caaggattcc tgggccgttt tgatcgtcgg atagccggtg
    10081 atcatcagaa caggagtggt gggccggtat gtccggaggg acttcaacaa gtcgaggccg
    10141 ctcagaccgg gcatcatcaa atcgcagata ataagatcgt agcggacggc ggccgtccgg
    10201 atgagcgcgt cctctccgga aaggacggtc tcaatttcga cgccttcccc ggccaaggcc
    10261 agggcgatgc tttggacaac ggagcgttca tcgtcgatga tcaagattct ccgttttgtc
    10321 tcggtcatgg tccaattcct ctgtccccgg tttccgtctc cgccagcggc cattgtatcg
    10381 taaagaccgc gccgcggcca gccccgtttc gggcggagat ggttccgccg tgctgctcga
    10441 cgaggctggc ggcgacggcc agccccaagc ccgttcccga ggatttggtc gtgaaaaagg
    10501 ggtcgaaaat ccggtcgagg ttttcctccg ggattcccgg ccccgaatcg gcgaatacga
    10561 cctcgatcga ccggccgtcg gccgagcggc ggctggaaac atcgatcgtt cctcccgagg
    10621 gcatcgcctc gcaggcgttg aggatcaggt tccagaacac ctgttggatt ttcgctctgt
    10681 cgagaaagag cggagggagc gtccggtcca aatgatggga gagacggacg ttgcgggccc
    10741 aggcctggcc ggaaagcagg ccgatggtgc gttccacgac ggcgttgacg tcgagggcgg
    10801 atttctcggg cggcgtccgc cgggagtatt cgagaagccc cttgacgatc cggccgcagc
    10861 gctcggtttc ctcggcgatc aggcggagag cggccgcggc cggatcgtcc ggccgagctt
    10921 tttccagcag aagatgggcg ttgatgagga tggtcgtcag gggattgttg atttcgtggg
    10981 cgatgccggc ggcgagttgc ccgagggagg ccattttttc cgatcgggcc atggattcct
    11041 ggatgttgcg gagggcgtgg gttcggtcgg cgacccggct ttcaagagtc cacgtccatt
    11101 ggagaagatt ttccttggcc ttgcgaagct cggcggtcat tcggttgaac gccccgccga
    11161 gctctccgat ttcgtcgcgg ccgccgaccg ggactttcag gttcaggtcg ccttgggcga
    11221 tccgggacgc ggccgcggcc aagtctccca acggccggac gagacgcgtc gccatgaggg
    11281 aaaggaggat cagcagaacg gccgccgtgg ccgcggccca ggcggcgttt tgggtgatca
    11341 cccggttgcg gagagcgaga aacggccgtt ctaaaagacc gatgctcagc attccgacga
    11401 cggtcccgtc gtaatcggcc aggggctcgg aagcgctgat gtaccgttcc ccgagaatga
    11461 cggcccgccc ggcccaagct ttccttcgtt cgagcgcctc ctgccggaag cggggatccg
    11521 cccgcgttcc gttcaaccgg ctcccctggg ggtttttgag ggttgtcgat gcccggatgt
    11581 cgccttggtg gatcgtgacg acgcccgtgt cccggccgcc gcgtttttcg ggatcgaaaa
    11641 tcgcggcttt gacccggtcg acgatctcgt tgtcgccgtt gatccgccgt ccgccctcca
    11701 ggacgccgat gatccttcct tccgggccgc ggacgggcgc cgccgcgaca aggacgagga
    11761 tgtccccggc cgcggattct tcgaaccggg tgctttcccg gcggagggaa tccaggggga
    11821 gaagccgcgt cgcggcgagc cgctttccct cgaggacccg ggagaaaaaa gaatcccggt
    11881 cccggaatcc gccggccgga acggccggat cggagagagc gatccgtctt ccgtcggcgg
    11941 ccgcgaacga tagaaaatca aaacgtcccg cttccgcggc ccgagccaga tggaaatcca
    12001 gttcggcgtt cggctgggcg gagcggggga gacgcaagag ctccgattcg gccgtcaggg
    12061 tcacggccgt cagcaggttg tccaagtaaa aggaataaat ggtccgggcg gagtccatat
    12121 cgtggtagat ccggttttcc gccagggaca tgatcgtctg gtgttcgagt cgcaggccga
    12181 ggatgccgaa aacaacccct ccgaagagaa cgatggcgag aatggcgagg atcatttttc
    12241 gcctgagagg gagatcgaca aaccggaaag gcatcttttc cgccgcagtt attttatcat
    12301 aaatgaactt gacagaaggc ccgggaaatg ataaatcaga tggccgattg gtatagtcaa
    12361 ctaataggat agttcttttg agactgtcga cgaaaggcga gtatgcgagc cgggcgatgc
    12421 tcgagctgac gttgcattac ggcggaccgc ctcttcacat ccgagacatt tccagggtcc
    12481 aagacattcc ggaacgtttc cttgagcaaa tcctcctcca aatcaaaagg gccggatacc
    12541 tgcgcagccg gaaggggccc aacggcggat atatgctctc caagccgccc gaagagatct
    12601 ccgtggcgga gatcatccgc gtcatggatg gtcccctcgc cccgatcgac tgcgtcagcg
    12661 tcaccgcccg cgaagtctgt ccacgggaaa aatcgtgcgg cctccgctgg ctttggaaga
    12721 gaaaccggga cgcgatcgcc gaaatcctgg aaagcacttc ctttgccgat gtcgcagaaa
    12781 tgacgcgaaa agcgatgagg gccaataatg aaaacggaaa tcaaaagacg ccggataagg
    12841 gataaaattt ttttaggaat agtcgatatt aacaatagac tatatcaact aaataccggg
    12901 aggtgatcat gtcgaaatgg aagaagacgg ccgggctcct gatcgcggtc cttgccctga
    12961 tcccggcggc tttcgccgag gaaaaaacgc cgcccccatc ggtgacggcg gggaaaaccc
    13021 tcaagctttc gggtttcacc caagtcctcg gcgctctgca ggaccaaggc gtcgacgcct
    13081 tgagcgtccg ccgttcgcgc ttcagcctgt ccgccgatct catgaagaat atcaaggcca
    13141 agttcacggt cgatgccgtc cgaaatccca tccttgtcga tgccatggtg gacgtggttt
    13201 tcgccgagtc tgcggctctc cgcttcggcc aattccgggt tcctttcggc gcggaaaatg
    13261 acacgtcctc ggccgacttg gatacgatta atttttccca acccgtcagc aagctggccc
    13321 cgggccgcga cgtcggcacg atgggccgcg acgtgggcgc catgctgacc ggacaagtct
    13381 ccgtttttga ttacagcctg gggctcttca acggcgccgg agccaacaag accgatacga
    13441 acgacgcgaa ggatttcgcc ggccgggtgg gggtgaagcc atttgacttt ctcacgatcg
    13501 gcgcgtccct ctacaaggga aaagccgacg ccgcggccgg agcgccgctc atccgccggg
    13561 atcggaccgg gctccacgcc ggcgtctctt tcggggacgt gtcgctgaaa ggggagatga
    13621 tctgggcggc cgacggcgag atggaccgcc ggggatggtt cgtccaagcc ggctggtttg
    13681 ttcttcccaa gcagctgcag atcgtcgcca aggtggatgc gtacgacaag aacacggctt
    13741 tggacgatga ccgaaccaac ctttataccg ccggtttgaa ttggatgatt tccggcaaaa
    13801 cgaagcttca ggttaattat gaatacctga gaaacgagct gggggggacg gtgaataaag
    13861 ccctcttggt tcaatttcag gccggattct gattaaagaa ggagagatcc atgaaaaaac
    13921 tgatgatcgc ggttctcctg gccgtctcgg tcctgggcgg ctttgccgcc ggcccggctc
    13981 aagcccccgt cctggaaggc caggtctcga tttccggcgc ctgggctctt tatcccatgg
    14041 tcgtcaagtg ggccgaggaa tttcagatat tgcatccccg ggtgcggatc gatgtccagg
    14101 ccggcggcgc cggcaagggg atcgccgatg tgatcgccga catggtcgat ttcggcatgg
    14161 tctcgcgcga cgttcacccc gtggagatgg agaagggcgc tctgccgttc gccgtcacca
    14221 aggacgccgt cgttcccatg atcaactcca agaatcccta tctccccgaa atcctgcgga
    14281 aagggctgac ccgccaggcg ttcatcgaca tttggattac gaagtcggcg accgtctggg
    14341 gcgacgtcct ggacggcaag gcgccggcgc cgattcatgt cttcactcgc tcggacgcct
    14401 gtggcgcggc cgaaacctgg gccttgtttc ttgacaagcg ccaagaagac ttgggcggcg
    14461 tggccgtcta cggcgatccc ggcttagccg acgccgtccg ccgggatccc ctcggcatcg
    14521 ggttcaacaa cgtcaatttc gcttatgacc cgaaaacgct ggccccgatc gccggcctag
    14581 tggccgtgcc catcgatctc gacggcaacg gcatgatcga tccggtcgag cggttctacg
    14641 ggacgcgcga tgacttgacg aaggccatcg gcgaaaacaa gtatccctct ccccccgccc
    14701 gggaccttta cctggtttcc aagggcgtgc cggccaagcc ggcggcggcc gccttccttc
    14761 ggtggatcct cggagaaggg caaaaatacg tccccgaaac cggttatatc cggctgagcg
    14821 aggacaagct caaggcggga ctcgcccttc tcgataaata agggatgaaa tcgacccctg
    14881 ttttctgata tgatgcgccc tttccggaga cggtcaaggc atggtcaaac ggctgtttaa
    14941 agagcttgcc gcccggcggc tgatgtttct cgtcaccatc ctgccggggg cgctcgtctt
    15001 tttgatgctg ttcgggctgt actcccggtc gcggagcatt cttgcccttc attctttttc
    15061 cgatcttctc ttcggttcgt cctggttccc cttgaagggc gaattcggct tgtacccgtt
    15121 cctcatgggg acggtctggg tgacgctggc ggccatggcc atcgccgtcc cggtttccct
    15181 gctgagcgcg atttacctgt ccgaatacgc gccgcggcgg attcgggaga ccatcaagcc
    15241 catcgtcgac ctcctttccg gcatcccctc ggttgttttc ggcgtctggg gcgtcctgat
    15301 gatcgtcccc tttgtggcga aaatcgctcc ggcgttcggg gcgtattcca ccgggtactc
    15361 ggtgctggcc ggagcgatcg tcctggccat catggtcttt ccgacgatca tccatgtctc
    15421 cttggaggtc ttcgcctcgg tgcctcaatc cctccgcgac gcctccctct cgctgggggc
    15481 gacgaaatgg gagacggtca agcacgtcgt tttacgcaag gggctggccg ggatcatcgc
    15541 ctccgtggcc ctcggatttt cccgggcctt cggcgaaacg atggccgtgc tcatggtcgt
    15601 cggcaacacg cccatcatcc cgaaatccgt cttcgacagc gcctatcccc tgccggctct
    15661 tctggccaat aattacgggg aaatgatgtc cattcccctg tacgacggag ctctgatgct
    15721 ggccgccttc gtccttctcc tgatcgtcct tgtgttcaac atcggcgccc gcgtggtcct
    15781 gatccgggcc gagcaaaagg cggaatgatg ggcttccggc ggcggaaact ggaggaatcg
    15841 atcttcaagg tgttgatgat cggctcgacc gtgatcgtca tcggaagcct tctcctcatc
    15901 atcgctacgg tcatcgtcaa gggattcccg gccatgaacc tggccatgat cactcagacg
    15961 cccaagggcg ggtactacct gggaaaagag ggcggcattc tcaacgccat cgttgggtcc
    16021 ctggagctgg ccggcggggc caccctcctg gctctcctgt tcagcctgcc gatcgtcctg
    16081 tacattaatc tctacaagcg gcggaaatcg cggtttgccg tcttcgtccg cttctccctg
    16141 gacgtccttt ggggcatccc ctccatcgtc tacggggcct tcggcttcat cctgatgatg
    16201 atggtcggcc tgcggacgtc gctcctggcg ggcatcatcg ccgtggccct cctggagctt
    16261 ccgatcatgg cccgggcgat ggacgaggtc gtccggctcg tcccctcctc gctccaggac
    16321 gcttcggcct ccctgggggc gacgaagctt gaaacggccg tccacgttat cgtccgtcag
    16381 tgccttcccg gcatcatcac cgcgattttg atcgccttcg gccgcggcat cggcgacgcc
    16441 gcctcggtct tgtacaccgc cggtttcagc gaccgcgtcc ccggggcgct cctccagccg
    16501 gcggcgacgc ttccgctggc gattttcttc cagctcggca cgccctaccc ggaggtccag
    16561 gagcgggctt atgcgtcggc cgcgatcctg acgatcatca ttctcgtcat cagccttctc
    16621 tcccggtggt acagccggaa attcagaaaa aactccgcca t
//
LOCUS       DAOOXH010000053        16296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_3493_length_16296_cov_7.644357, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000053 DAOOXH010000000
VERSION     DAOOXH010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 16296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16296
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3493_length_16296_cov_7.644357"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2872
                     /locus_tag="PKV64_07345"
     CDS             <1..2872
                     /locus_tag="PKV64_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16929.1"
                     /translation="TLFRYKIRDFDLVYYAQAHEYIIEHLARSYLRTMDFYKKFFDYR
                     PTQETAVFLRDFGDWGNGGATAVPRNLVFFELSPFDHVYDILNAYERISLVMHHEMVH
                     VITMDKPVGAASFFRKLFRGKVMPDKENPLSMFYTYLTSPRAYAPRWYLEGIAVFMET
                     WTDGGLGRSLGAYDEMMFRTEVLEDGLLYDALGLESEGTAVDFQIGALSYMYGTRFFS
                     HLGAKYGPEKVVAWTSAEQGSKGYFAAEFKRTFGLPLTREWSAWIAAEKTWQEDNLAR
                     IRGNPVTTFKPLTTERLGSVSRAFHDPGRGKVYLGLNYPGQIPHLAAVDVKTGAIERL
                     AEIKGGKLHSVCSLAFDREGGRLFFTSDNNSFRDLRVLDLATGRTKLLIKDLRAGDLI
                     FNPADQSLWAIRHNLGLSSLIKVDPPYADWTALYGFDYFADVYDLDLSPDGLFLTAAS
                     ADVSGDQRLVRVRTADVLQGRWEPETLFDFGKDAPASFRFSPDGRFLYGSSYYTGVSN
                     IFRYDLERKTLDALSNCETGFFKPVPAAEDGPLLVFKYAKDGFVPGWIPGRPVEDVNA
                     IRFLGQEVVDRHPAVKNWTIGSPADIRLENLTVETGVYNSFKDIRLNSISPILEGYKG
                     SLAPGLRVEWRNPLRFNGFDLTASYSLDPSLPARERPHLGLAYHFWNWTFAATYNNAS
                     FYDLFGPTMAGRKGYSLAARYSREMIYDPPRSLRIEAQAAAYAGLDRMPDYQNIDATY
                     DKLLTARAGIKYADLRRSIGAVEDEKGPLAHFFVQANGVNGRIYPRVYGRFDYGIALP
                     LNHSSLWLRTSAGRSFGDRTNDFVKFFFGGFGNNWIDAQEENRYREQAGFPGVGINSI
                     GGRSYAKGVVEWLSPPIRFRRLGWPSLYCTWARLALFAAGIATDIDIPSAGREVAGWG
                     AQLDFRVVLFSTFNTTLSLGCARSYEKGRPARNEFMLSLKLF"
     gene            complement(2895..4664)
                     /locus_tag="PKV64_07350"
     CDS             complement(2895..4664)
                     /locus_tag="PKV64_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPN16930.1"
                     /translation="MRKFTRIALMAAVVLSAGFAGSGGVPDSAGAEVVLSFDHWYDNA
                     AIGRALRDLQAAYPGLITVQSIGKSVLGRDIWAAVLNNPKTGPAERKPGYYIDGNIHG
                     NEIQGGEVALYTVYYLLKNHGRTEFATDVLDRVAFYVVPSMNPDSRDFYIHEASDPNS
                     PRTGWIPRDDDRDGVADEDGPEDLNGDGSITTMRKRDLNGRLRAHPDDPRLMIPVKPG
                     QKGEWTLLGEEGIDNDGDGLINEDGPGGYDMNRNFGYNWQPEYVQGGAGPYPFSWPET
                     RAVRDFILAHPNIMGAQSFHNNGGMILRGPGAGNLGDYNPADVRVYDEIGRRGEKILP
                     GYKYIIIAKDLYTVYGGTVDFLYASLGAFTFSNELDLDLVEEIRLQKPENADEEERSR
                     SFFRGLSKEDMILHDQVFMGEHYAPWKPYVHPVYGEIEIGGVKKFGERVPPLFKLAET
                     CHRNAAFVLYHASEMPAVAFEDLSVKTLDGGLFQVDFAVTDAKATPTMSAQAASRKLH
                     RPDRIRIGGPTAELVAVGEMRDRFLNETRAIKPAGKAFDVSGGIPGRGRREFRILVMG
                     SGPLTLSYDSLKGGSAETTVELK"
     gene            complement(4675..6729)
                     /locus_tag="PKV64_07355"
     CDS             complement(4675..6729)
                     /locus_tag="PKV64_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPN16931.1"
                     /translation="MNRKPLSGFLGLLVFAAAAFGQAPAGNLGGPFRKPAEAVSALKD
                     LAAGFPAVAALEIIGKSAGGREIPVLRLAGGNPAVKTAPGLRPAVLITAGLDGGHVAG
                     TEAALRLAGKLVDGYVKKDGKITALLDARTVYVAPLLNPDGAEAYFGAVRSGRFINAR
                     PVDEDADGAVDEDGFEDLDKNGAITQMRMKDPEGVFIPDPKEPRLLRPADPAKGEKGI
                     YKVFPEGIDNDGDGEYNEDPPGGVQLNRNYPHDFEYFVKTAGLYPASEPETAALLQFM
                     TDHPQIGLVLAFGLENTILNQKQTGQAKAGSDKVKPIRPIAELIGLDPEKEYTLKEIA
                     EAATASGIGGGMTITEEMVAGFLGVGPAMEIDKADLPFLEAVQKDYKEVLKAAGKEGL
                     EKHARGAGKGSFGGYVYFQYGVPFFSTDLWQVPEPKKEEPADKLTAEKLKAMTAAQFL
                     ALGEEKIAVFLKDSGAPPQFNSRAVMEMVESGKLTTIRMAEMMAQMPPRPGAGSEEHP
                     DAYKLQWADAVWGGRGFTNWTPFKHPQLGDVEIGGFCPHFGLNPPADEADAAIGVHVD
                     FYLKIMDNLPVLEISGIKAEPLGGGVYGLTAYVSNTGRWPTSTAQGRRARTAWPIRIT
                     LVLDKSQTLFSGRPAEEIPFLDGGETKKVEWTIRAKPGSRVAVKAWSPRLGAVEKTAV
                     VS"
     gene            6858..9983
                     /locus_tag="PKV64_07360"
     CDS             6858..9983
                     /locus_tag="PKV64_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16932.1"
                     /translation="MMRFRALRSSWLAGVLCVLAVSTAAAQTAAPMEELLRPFAWRSV
                     GPALPGGRTTDIEAVEAKPWIIYAAVGPSGVWKSENAGTSWAPVFHKEATVSVGDLAV
                     SQANPDIIWAGTGEATCRNSVTLGDGVYKSIDGGKTWTNQGLRETRHISRILINRGDP
                     NIVTVAAMGSLWNANPERGVFKTIDGGKTWKKILYIDENTGIADLAADWSDSQILYAA
                     AYEHRRLPWIYTSGGKGSGLYRSTDGGETWTKMTKGLPEGVLGRIGLDTSRSKPGVVY
                     AVVEAKDGGIFRSDDTGETWIRTCPTDVANWVANRPFYYSQIRVDPTNDEKLYVLATG
                     LSVSRDRGRHFQGIGGGTHSDHHSLWIDPANPLHLIEGNDGGIDISWDGGKSWDPVQS
                     IDAAEVYQVGYDFQKPYYVYCGLQDNNTWGGPSASLDAGGVRNEDWFILAGGDGFFAR
                     PDPNDPNLIYSNSQVNGITRFDRRINRPNGIRPEAPLDQPAYRYNWNSPIHLSPHDSH
                     VVYCGAQFLLRSPDQGRTWEVLSPDLTTNDPAKLRESGGPITPDNSGAESHCTITSIA
                     ESPVQAGVIWAGTDDGNVQVTRDGGKTWVNTVKAIPGLPPNTWCSRVEASHFEAGTAY
                     AAFDGHRTGDWGVYLYQTKDFGRTWKSLKANLPSFGWVHVVREDPRNPRLLYAGTEFG
                     VFGSLDGGASWFSLKSNLPTVAVHDIAVHPRDNDLIIGTHGRGVWIMDDIAFLQESTP
                     EVLESAGHLFSVRPATRYFFSSVREGYSRPPFAAKNPPAGLILTFYAKTAPKERPLMK
                     IIDAAGKTLAEIKFSTLAGYQRDTWNLNFVPERDGVKRIPAAAAMYGMPSVPPGEYTL
                     SLNVDGRVTESKTAVLADPRLDVPADVFAAQIEGISRSSTQLARLAQAVTALRSLRDQ
                     IPKIQEAVGKAGEIAAGLSPVLAGLKARVDALAEEVLPKDFIGLGSREQMLRGGTPFQ
                     KLTAFSINLANSAEPPTAVELRQIAELEKVTGDQVDRLNALLRTDVPPLNESLKKAGL
                     AVSLRVPAEIK"
     gene            complement(10008..11540)
                     /locus_tag="PKV64_07365"
     CDS             complement(10008..11540)
                     /locus_tag="PKV64_07365"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HPN16933.1"
                     /translation="MRRERQRGCPGSPRAEAKGGPMIPALRPKLELLSPALMDRILDE
                     AFLILGRDGVFFENAEARALFREAGQRVDETAQRVRLSRDLVERSLETTPPSLTLYDR
                     SGEKSFHVGGDEVHFDPGSSGTRILDHDTLRERRPNTADLAGIARLTEVLDHLHFQST
                     SLVPADVPGGIADDYRLYLSLLFGTKPVVTGTFRTAGFRPMHEMLCAVRGGAEALKAK
                     PLAIFDACPSPPLRWSLLTSQSLIDCARAGIPSELISMGMTGATSPATIAGTLVQHVV
                     ENLAGLTLAQLAYPGAPVIFGGSPSSFDMRQGTTPMGAMETMMIDCAYAQLGKRMKLP
                     THAYMALSDAKTNDAQAGYETGIGAVLAALAGINVVSGPGMLDYESTISLEKLVIDDE
                     ICALAYRLIDGVVPREEVLAAHLFEGFGPQTNFLTMPHTRKWYRKEHLVPIVADRELY
                     DAWEARGASSIADRAAGRVADLLEKNPPRLPESGLASELRRIMEADARANGVTALPRL
                     PD"
     gene            complement(11474..12130)
                     /locus_tag="PKV64_07370"
     CDS             complement(11474..12130)
                     /locus_tag="PKV64_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HPN16934.1"
                     /translation="MTKDIFSVMAMSVVDGLPETAGELARSALAGGTPALEAVEKGFL
                     PGIRKVGELWESGEYFLPELIASAEAMKAAMSVLRPALEKGGGTARSAGKIVIGTVEG
                     DIHDIGKNLVAAMLSAHGFEVVDLGADVKLVSFIETAEAEKADIICLSALLTTTMIGQ
                     RRLIDLLVEHGLRHRFQVMIGGAPTTRAWAEEIGADGYAENANAAVREALELRQKAAP
                     "
     gene            complement(12127..13944)
                     /locus_tag="PKV64_07375"
     CDS             complement(12127..13944)
                     /locus_tag="PKV64_07375"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HPN16935.1"
                     /translation="MRLIVSLFPSGRRLPADIGELLSDVLIRDGVPLNLYCGRRGVCG
                     KCFVEILSGEVPPGDESERDLAREKNLGLGRHRLACRLRVQGDLVVRVPEQALIPRAR
                     ILTRGRGREIPLDPMVRKVTVRSVRPDLQSPRALLDGLKAGLGLSALRVVPEALFEAA
                     GVLSEALESDSPVTAVLGAGDELRGVERGNTSSRLFGLAVDLGTTTVAADLVDLGTGT
                     VIKTAGGLNGQSVFGADVVSRITAAFKDMSRAAALRAAVLASINGLILELSRESGVDP
                     GEIYETVVAGNTAMSHLFLGLPVDGLAVAPFAGVFTAAPPLPADRTGLKINPLGRVYL
                     SPNLQSFVGGDVAAGLVATDLAARPGNVLFVDLGTNGELVLKTDRTMMSTSTAAGPAF
                     EAASISCGLPAEDGAVERAVLRDDGTIVLETIGGGPARGVCGTGLVDLIAAFLRRGDL
                     APDGRILRLEKRLAAAPDLFLTASDIREFQLAAAAVKTGIRMLLEAAALNIEDLNEIL
                     VAGAFGARLDVENAAAVGLLPRLPGDRVKFVGNTSLEGARALLLSSRERLRAESLPRV
                     IRHLPLAQNEAFQNIFIKELPFKPWPPDEAPQAPRKEDS"
     gene            complement(13946..15343)
                     /locus_tag="PKV64_07380"
     CDS             complement(13946..15343)
                     /locus_tag="PKV64_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPN16936.1"
                     /translation="MGLFEASSLVVGSVIGSGIFMTSGLIAEFLPYSMPMLAAWLAAG
                     IVTMIGALCYGELGSMFPRAGGPYVYLKEAYGAAPAFLYGWAFFFLIGGAGVAAIATG
                     FAEYFGAVFPVFESGRLMMSGRLFSLDIQITAGRLISIAAVIGLTLFNGSGLRKSARG
                     QSILTIIRVVLLLGFVAIGAWYGIKTRAVNLKPLFPPAGAWPGWSAFGAALITALWSF
                     DGWYAASCAAEEIRDPRRNVPRSLLLGTASVLILYLAVNIVYSMALPMERMRGVVRVG
                     QEAVAAMFASAPQGTAAPAFAGVVALTIFGCLSANIFFCARVPYAMARDGLFFRRLGI
                     LHPVKRVPDKALWAEAAVAVILILTGTFQKLIDYVLFGLVLFFSATGAAVIVLRRRAP
                     AVERPYRLRPYPLLPLAFAAVNGAILVALAVEHPGQAAVAAGLIGTGIPAYFLWKGRR
                     RRPQSGTGGPRAGEA"
     gene            16112..>16296
                     /locus_tag="PKV64_07385"
     CDS             16112..>16296
                     /locus_tag="PKV64_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPN16937.1"
                     /translation="MVLIIAVTNQKGGVGKTTTAVNVSAALAFKGKRVLLVDTDSQGH
                     STKSIVVESERFEHSLY"
BASE COUNT         3037 a         5141 c         5008 g         3110 t
ORIGIN      
        1 gacgctcttc cgctataaaa tccgggattt cgacctcgtc tattatgccc aggcccacga
       61 atacatcatt gaacacctcg cccggtccta cctccggacg atggacttct acaagaagtt
      121 cttcgactac cggccgaccc aggaaacggc cgtgtttctc agggatttcg gcgactgggg
      181 caacggcgga gcaacggccg tgccccgcaa cctggttttt ttcgagcttt cgcctttcga
      241 ccatgtctac gacatcctca atgcctacga gcgcatctcg ctggtcatgc atcacgagat
      301 ggtccacgtc atcaccatgg acaaacccgt cggggccgct tcattcttcc gcaagctgtt
      361 ccggggcaag gtcatgcccg acaaggaaaa ccccctgtcc atgttctaca cctacttaac
      421 gagcccccgg gcgtatgccc cgcggtggta cctggagggg atcgccgtct tcatggagac
      481 ctggacggac gggggcttgg ggcggagtct cggggcctac gacgagatga tgttccggac
      541 ggaagtcctt gaggacggcc ttctctacga cgcccttggg ctggaatccg agggaacggc
      601 cgtggacttt cagatcgggg ctctgtccta catgtacggc acccggtttt tctcccactt
      661 gggggcgaaa tacggcccgg agaaagtcgt cgcctggacg tcggctgaac aaggaagcaa
      721 gggatatttc gcggccgagt tcaagcggac gttcggtctg cccctcaccc gggaatggtc
      781 ggcctggatc gcggctgaaa aaacctggca ggaggataac ctggcccgga tccgcgggaa
      841 tccggtgacg accttcaagc ccctgacaac ggagcggctg ggatcggtgt cccgggcctt
      901 ccacgatccc ggaaggggaa aggtttacct cgggctcaat tatcccggcc agattcccca
      961 cctggcggcc gtcgacgtca aaaccggggc tatcgaacgt ttggccgaaa tcaagggcgg
     1021 gaagctgcac tccgtatgtt ccctggcttt cgaccgggag gggggacgcc tctttttcac
     1081 ctcggacaac aattcattcc gcgacctgcg ggtcctggac ctggcgaccg ggcggacgaa
     1141 gctcctgatc aaggacctgc gggccgggga tctcatcttc aatccggccg accaaagcct
     1201 ctgggcgatc cgccacaatc tcggcctttc ctccctcatc aaggtcgatc cgccgtacgc
     1261 cgactggacg gccctttacg gcttcgacta cttcgcggat gtttacgatc tcgacctttc
     1321 gcccgacggc ctcttcttga ccgcggcctc ggccgacgtg tccggggatc agcgcttggt
     1381 ccgcgtccgg accgccgatg tcctccaagg ccgatgggaa ccggagacgc tcttcgactt
     1441 cggcaaggac gccccggcgt cttttcgctt ttcgcccgac gggcggtttc tctacggctc
     1501 ttcctattac accggggttt cgaacatctt ccgttatgat ctcgagcgga aaaccctgga
     1561 cgcgctcagc aactgcgaaa ccggcttttt caagccggtt ccggccgccg aggacggacc
     1621 gctcctggtc ttcaagtatg ccaaggacgg ctttgtcccc ggatggatcc ccggccgtcc
     1681 cgtcgaggac gtaaacgcca tccggttcct cggccaggaa gtcgtcgacc ggcatcccgc
     1741 ggtcaagaat tggacgatcg gttctccggc cgacatccgc ctcgaaaacc tgaccgtgga
     1801 aacgggcgtc tacaattcct tcaaggacat ccgcttgaat tcgatctctc ccatcctcga
     1861 ggggtacaag ggctcgctgg ctcccggcct gcgggtggaa tggcgcaatc cgctccgatt
     1921 caacggattc gatctcaccg cgtcctattc cctcgatccc tcgctcccgg cccgggagcg
     1981 cccccatctc ggcttggctt atcatttctg gaactggact ttcgccgcga cctataacaa
     2041 cgccagtttt tacgaccttt tcggtccgac catggccggc cgcaaaggat attccctggc
     2101 cgcccggtat tcgcgggaga tgatctacga tcctccccga agcctccgga tcgaggccca
     2161 ggcggcggcg tacgccgggc tggacaggat gcccgactat cagaacatcg atgcgacata
     2221 cgacaagctg ctgaccgccc gggccgggat caagtatgcc gatctccgcc ggtcgatcgg
     2281 agccgtcgag gacgagaaag ggccgctggc tcatttcttc gttcaggcca acggcgtcaa
     2341 cggccggatc tatccccggg tttacggacg gtttgattac gggatcgccc tgcccctcaa
     2401 ccactcctcg ctctggctcc ggacatcggc cggccggtcg ttcggcgacc ggacgaacga
     2461 tttcgtcaaa ttcttcttcg gcggattcgg gaacaactgg atcgacgccc aggaggaaaa
     2521 caggtaccgg gagcaggccg gctttccggg cgtcgggatc aactccatcg gcggccggag
     2581 ttacgccaag ggcgtcgtcg aatggctttc gccgccgatc cgcttccgcc gcttgggatg
     2641 gccgagcctc tactgcacgt gggccaggct cgccctcttc gccgccggaa tcgcgacgga
     2701 tatcgacatc ccgtcggccg ggcgggaagt ggccggctgg ggcgcccagc ttgatttccg
     2761 ggtcgttctg ttttcgacct tcaacacgac cctgtcgctc ggctgcgccc ggtcttacga
     2821 aaaaggccgt cccgcccgga acgagttcat gctctccctg aagctctttt aaacggcaga
     2881 ccgccgccgg ccgcttattt cagctcgacc gtcgtttccg cggatccgcc cttcagcgaa
     2941 tcgtagctca gggtcagcgg ccccgatccc atgaccagaa tccggaattc ccgccggccg
     3001 cggccgggga ttccccccga tacgtcgaac gcttttccgg ccggcttgat cgcccgggtc
     3061 tcgttcagga aacggtcccg catttcgccg acggccacaa gctccgccgt cgggccgccg
     3121 atccggattc ggtcgggccg gtgaagcttc cggctcgccg cctgggccga catcgtcggc
     3181 gtcgccttgg cgtcggtcac ggcgaaatcg acctggaaca agcctccgtc aagcgtcttg
     3241 acggagagat cctcgaaggc cacggccggc atttcggagg cgtgatacag cacaaaagcc
     3301 gcgttccggt ggcaggtttc ggccaacttg aagaggggag ggacccgctc cccgaatttc
     3361 ttgacgccgc cgatctcaat ctcgccgtaa accggatgga cgtacggctt ccagggcgcg
     3421 taatgctcgc ccatgaacac ttggtcgtga aggatcatgt cctccttgga cagtccccgg
     3481 aaaaaggacc ggctccgctc ctcttcgtcc gcgttctcgg gtttttggag cctgatctcc
     3541 tcgacgagat cgaggtccag ctcgttggag aaagtgaacg cccccaggct tgcgtagaga
     3601 aaatccacgg tcccgccgta gacggtgtag aggtccttag cgatgatgat gtatttatag
     3661 ccgggaagga ttttttcgcc gcgccggccg atttcatcgt agacccggac atcggccggg
     3721 ttgtaatcgc cgagattccc ggctcctggt ccgcgcagga tcatcccgcc gttgttgtga
     3781 aagctctgcg ctcccatgat gttcggatgg gccaggatga agtcgcggac ggcccgggtt
     3841 tcgggccagg agaacggata ggggcccgcc ccgccctgga catactcggg ctgccagttg
     3901 tagccgaagt tgcggttcat gtcatagccg cccggcccgt cctcgttgat cagcccgtcg
     3961 ccgtcgttgt cgatcccttc ctcgccgagc agcgtccact cgcccttctg tccgggtttg
     4021 accggaatca tgaggcgggg atcgtcggga tgcgctctca gccggccgtt gagatcgcgc
     4081 ttccgcatcg tcgtgatgga tccgtcgccg tttagatcct cgggaccgtc ctcgtcggcg
     4141 acgccgtccc gatcgtcatc gcgggggatc cagcccgtcc gcggagagtt gggatcggat
     4201 gcctcatgaa tgtagaagtc ccggctgtcg ggattcatcg agggaaccac gtagaaggcg
     4261 actcggtcaa ggacgtccgt ggcgaattcc gtccggccat ggtttttcaa gagataatag
     4321 accgtgtaca gggcgacttc gccgccctgg atctcgttgc cgtggatgtt gccgtcgatg
     4381 tagtagccgg gcttgcgctc ggccgggccg gttttgggat tgttgaggac ggccgcccag
     4441 atgtcccggc ctaagacgga tttcccgatc gattggaccg tgatcagccc ggggtaggcc
     4501 gcctgcaggt cgcgcagggc ccggccgatg gccgcgttgt cgtaccagtg atcgaagctc
     4561 aggacgacct ccgctccggc cgagtccgga acgccgcccg atcctgcgaa gccggccgat
     4621 aaaacgaccg ccgccatgag agcgattcgc gtgaatttgc gcatgtccgc ctcctcagct
     4681 cacaaccgcg gttttttcga ccgcgcccag ccggggggac caagccttga ccgcgacccg
     4741 ggatcccggt ttcgcccgga tcgtccattc cacttttttc gtctcgccgc cgtcgaggaa
     4801 cggaatctcc tccgccggcc ggcccgaaaa cagcgtttgg gacttgtcca ggacgagggt
     4861 gatccggatg ggccaggccg tcctggcccg ccgcccctgg gcggtcgacg tcggccagcg
     4921 gccggtgttg gagacgtagg ccgtcaatcc gtatacgccg ccgccgagcg gctcggcttt
     4981 gatcccggag atttccagga ccgggaggtt gtccattatt ttcaagtaaa aatccacatg
     5041 gacgccgatc gccgcgtccg cctcatcggc cggcggattg agtccgaagt gagggcagaa
     5101 tccgccgatt tcgacgtccc cgagctgggg atgcttgaac ggcgtccaat tcgtgaagcc
     5161 gcggcctccc caaaccgcat cggcccattg gagtttgtag gcgtcgggat gttcttcgga
     5221 cccggccccg ggccggggag gcatctgggc catcatctcg gccatccgaa tggtggtgag
     5281 tttgccggac tcgaccattt ccatcacggc ccggctgttg aattggggag gggcgccgga
     5341 atccttcagg aagaccgcga ttttttcctc gcccagggcc aggaactgcg cggcggtcat
     5401 cgccttcagc ttctcggccg tgagcttgtc cgccggctcc tcttttttcg gttcgggaac
     5461 ctgccacagg tcggtcgaga aaaacggcac gccgtactgg aaataaacgt agccgccgaa
     5521 cgagcccttg cccgcgccgc gggcatgctt ctccaggcct tccttcccgg ccgccttgag
     5581 gacctcttta tagtccttct gaacggcctc gaggaaaggc aggtcggcct tgtcgatctc
     5641 catggccggg ccgaccccga ggaagccggc gaccatctcc tcggtgatcg tcatcccgcc
     5701 cccgatcccg gacgccgtgg ccgcctcggc gatttccttc agtgtgtatt ccttctcggg
     5761 atcaagcccg atgagctcgg cgatcggccg gatcggcttg accttgtcgg agccggcctt
     5821 ggcttggccg gtctgctttt ggttgagaat cgtgttctcc aaaccaaagg ccaggaccag
     5881 gccgatctgg ggatgatcgg tcatgaactg aagaagagcg gccgtttccg gctcggaggc
     5941 cgggtacagg ccggctgttt tgacaaaata ttcgaaatcg tgcggatagt tccggttcag
     6001 ctggaccccg ccgggcggat cctcgttgta ctcgccgtcg ccgtcgttgt cgatcccctc
     6061 gggaaagacc ttgtagatgc ctttttcgcc cttggcggga tccgccggcc ggaggagccg
     6121 gggctccttg ggatccggga taaaaacgcc ttccggatcc ttcatccgca tctgggtgat
     6181 cgccccgttc ttgtccaggt cctcgaagcc gtcctcgtcg acggctccgt cggcatcttc
     6241 gtcgaccgga cgggcattga tgaagcggcc cgagcggacc gccccgaagt acgcctccgc
     6301 tccgtccgga ttgagcaagg gggcgacata gactgtccgg gcgtcgagaa gagcagtgat
     6361 cttcccgtct ttcttcacgt atccatcgac cagttttccg gccaggcgga gggccgcctc
     6421 ggtcccggcg acatggccgc cgtccagtcc ggccgtgatc aacacggccg ggcggagacc
     6481 gggcgcggtc ttgaccgccg gattgccgcc ggcgagccgc aggaccggga tctcccggcc
     6541 gccggcgctt ttcccgatga tttccagggc ggccacagcg gggaacccgg ccgcgagatc
     6601 cttcagggcg gaaacggctt ccgccggctt gcggaacggg ccgcctaggt ttccggccgg
     6661 cgcctgcccg aaggccgcgg ccgcgaaaac caggaggccg aggaagccgc tgaggggttt
     6721 ccggttcatt cgttttctcc ttaacctgtc gccgccgggg acggcaggat taaactcgca
     6781 agaacatttc gcgtatttta taatgatcta tacgaaaaaa acgaggaaag agttactcga
     6841 aggaggatcc catgatcatg atgagattcc gcgccctgcg gtcgtcatgg ctggccggcg
     6901 ttctttgcgt cctggccgtt tccacggccg cggcccaaac cgccgccccg atggaggaac
     6961 ttctccggcc attcgcctgg agatcggtcg gaccggccct gcccggcgga cggacgacgg
     7021 atattgaagc ggtcgaggcc aagccgtgga tcatctacgc ggccgtcggc ccgagcggcg
     7081 tttggaaatc cgaaaacgcc ggaacgtcct gggcgcccgt gttccacaag gaggcgaccg
     7141 tttcggtcgg cgacctcgcc gtctcccagg cgaatcccga catcatctgg gccgggacgg
     7201 gcgaggcgac ctgccggaat tcggtcacgc tcggcgacgg cgtttacaag tcgatcgacg
     7261 gcggcaaaac ctggacaaac caaggcctcc gcgagacccg gcacatcagc cgcattctca
     7321 tcaaccgcgg cgatcccaac atcgtcaccg tcgcggccat gggatccctc tggaacgcca
     7381 atcccgaacg cggcgtcttc aagacgatcg acggcggcaa gacgtggaag aaaatccttt
     7441 atatcgacga aaacaccggc atcgccgacc tggccgccga ttggtccgac agccaaatcc
     7501 tctatgccgc ggcctacgag caccggcgcc ttccctggat ctacacgagc gggggaaagg
     7561 gcagcggcct ttatcgctcg accgacggcg gggagacatg gacgaaaatg accaaaggcc
     7621 tgcccgaggg cgtcctcggc cggatcggtc tcgatacctc gcggtccaag cccggcgtcg
     7681 tttacgccgt cgtcgaagcc aaggacggag gcatcttccg ctcggacgat acgggcgaaa
     7741 cctggattcg gacatgcccg accgacgtcg ccaactgggt cgccaaccgg ccgttctatt
     7801 acagccagat ccgggtcgat ccgaccaatg atgaaaagct ttacgtcctg gccaccggcc
     7861 tgagcgtttc ccgcgaccgc ggccggcact tccagggaat cggggggggg acgcactccg
     7921 accaccattc cctctggatc gacccggcca accctcttca cctgattgag ggcaacgacg
     7981 gggggatcga catctcctgg gacggcggca aatcatggga tcccgttcaa agcatcgacg
     8041 cggccgaggt ctaccaagtc ggatacgatt ttcagaagcc atactatgtc tactgcggcc
     8101 tgcaggacaa caacacctgg ggcggcccca gcgcctcgct ggacgccggg ggcgtccgga
     8161 acgaggattg gttcatcctc gccgggggcg acggcttctt cgcccggccc gacccgaacg
     8221 acccgaacct catctattcc aactcccagg tcaacgggat cacccggttc gaccgaagga
     8281 tcaaccggcc caacggcatc cggccggagg cccctctcga ccagccggcg taccggtaca
     8341 actggaattc gccgatccac ctgtcgcccc atgattccca tgtcgtgtac tgcggggccc
     8401 aatttcttct ccggtcgccc gaccagggac ggacctggga ggttctcagc ccggacctga
     8461 cgaccaacga cccggccaag ctcagggaat ccggcggacc cattaccccg gacaattccg
     8521 gggcggaaag ccactgcacg atcacctcga tcgccgaatc gcccgttcaa gccggcgtca
     8581 tctgggccgg gacggacgac ggcaacgtcc aagtcacgcg ggatggcggc aaaacttggg
     8641 tcaacacggt caaggcgatc ccgggccttc cgccgaacac ctggtgttcc cgggtggaag
     8701 cttcccattt cgaagccggg acggcttacg cggcttttga cgggcaccgg accggcgact
     8761 ggggggttta tctttatcag acgaaggatt tcggccgaac atggaaatcg ctcaaggcga
     8821 atcttccatc cttcggctgg gttcacgttg tccgcgagga tccccgcaat ccccggctgc
     8881 tgtatgccgg cacggagttc ggcgtgttcg gctccctgga cggaggggcg tcctggtttt
     8941 ccttgaagag caatctcccg accgtggccg tccacgatat cgccgtccac ccgagggaca
     9001 acgacctgat catcggaacc cacggccggg gcgtctggat catggacgac atcgcctttc
     9061 tccaggagtc gactcccgag gtcctggaaa gcgccgggca tcttttctcc gtccggccgg
     9121 ccacccggta tttcttctcg tccgtccggg agggatattc ccggcccccg tttgcggcca
     9181 agaatccccc cgccggcttg atcctgacat tctatgccaa aaccgccccc aaggagcggc
     9241 cgctgatgaa aatcatcgac gccgcgggca aaacgctggc cgaaatcaaa ttttccactc
     9301 ttgccggata ccagagggac acgtggaatc tgaatttcgt cccggaaaga gacggcgtca
     9361 agcggatccc ggcggccgcc gcgatgtacg gcatgccgtc ggttccgccc ggggaataca
     9421 cgctctcgct gaacgtcgac ggccgggtca cggaatcgaa gacggctgtt ttggccgatc
     9481 cgcgcctcga cgttcccgcg gatgtcttcg cggcccaaat cgaggggatc agccgttcgt
     9541 ccacccagct ggcccggctg gcccaggccg tcaccgccct gcggagtctc cgtgaccaga
     9601 ttccgaaaat ccaagaggcc gtgggcaaag cgggcgaaat agccgccggc ctttcccccg
     9661 tgctggcggg actcaaggcc cgggtcgacg ctttggccga ggaggttctt ccgaaggatt
     9721 tcatcggctt gggttcccgg gaacagatgc tccggggggg aacgccgttc caaaagctga
     9781 ccgcgttcag catcaatcta gccaattcgg ccgagccgcc gacggccgtc gagcttaggc
     9841 agatcgccga gctcgaaaaa gtcaccggag accaggtcga ccggctgaac gcccttctcc
     9901 ggacggatgt gccgcccttg aacgagagct tgaaaaaggc cggcttggcc gtttccctcc
     9961 gggtgccggc ggaaatcaag taaggccgga tccggccggg acgggcctca gtccggcagg
    10021 cgcggaagcg cggtaacgcc gttcgcccgg gcgtcggcct ccatgatccg gcggagctcg
    10081 gacgcgaggc cggattcggg gagccgcggc ggattcttct cgagaagatc ggcgacccgg
    10141 ccggcggccc ggtcggcgat cgaggaggcg ccccgcgctt cccaggcgtc gtagagctcc
    10201 cggtcggcga cgatcggaac gagatgctcc ttccggtacc atttccgggt gtggggcatc
    10261 gtcaggaaat tcgtctgcgg gccgaagccc tcgaaaagat gggcggccag aacctcctcg
    10321 cgggggacga cgccgtcgat caggcgataa gccaaggcgc agatctcgtc gtcgatgacc
    10381 agcttctcca ggctgatggt gctttcatag tcgagcatcc cggggccgga gacgacgttg
    10441 atgccggcga gggcggccag aacggctccg attcccgtct cgtagccggc ctgggcatcg
    10501 tttgttttcg cgtcgctgag ggccatgtag gcgtgggtcg gaagcttcat acgctttccc
    10561 aactgggcgt aggcgcagtc gatcatcatg gtttccatcg cccccatcgg ggttgtcccc
    10621 tggcgcatgt cgaagctcga cggagatccg ccgaaaatga ccggggcgcc ggggtaggcc
    10681 agctgggcga gggtcaggcc ggccaggttt tctacgacat gctgaacgag cgtgccggcg
    10741 atggtcgccg gcgaagtcgc ccccgtcatc cccatggaga tgagttcgga ggggatgccg
    10801 gcccgggcgc agtcgatcag gctctgggac gtcaggaggc tccagcgcag gggaggggag
    10861 gggcaggcgt cgaaaatcgc caggggcttg gccttgaggg cctccgctcc gccgcggacg
    10921 gcgcaaagca tttcgtgcat cggccggaag ccggccgtcc ggaacgtccc cgtcacgacg
    10981 ggcttcgttc cgaataaaag ggagagatac agccggtaat cgtcggcgat cccgccgggg
    11041 acgtcggccg ggacgagcga cgtgctttga aaatgcaggt ggtccaggac ctcggtcagg
    11101 cgggcgattc cggccaagtc ggccgtattt ggccgccgct cccgtagcgt atcatggtcg
    11161 aggatccggg tgcccgagga gccggggtca aaatggactt catcgccgcc gacatggaaa
    11221 gatttttccc cggaccggtc gtacagggtg agggacgggg gagtcgtctc caaggagcgt
    11281 tcgaccaggt cgcgcgaaag ccggacgcgc tgggccgtct cgtcgacgcg ctggccggct
    11341 tcccggaaca aggcccgggc ctcggcgttc tcgaagaaaa cgccgtcgcg tccgagaatc
    11401 aggaacgctt cgtccaggat ccggtccatc aaggccgggg agaggagctc cagcttgggg
    11461 cgcagggcgg gaatcatggg gccgcctttt gcctcagctc gagggcttcc cggacagccg
    11521 cgttggcgtt ctcggcgtat ccgtcggccc cgatttcttc ggcccaggcg cgtgtcgtcg
    11581 gggctccgcc gatcatgacc tggaacctgt gccgcaggcc atgctccacg aggagatcga
    11641 tcaggcggcg ctggccgatc atcgtcgtcg tcagaagggc ggagaggcag atgatgtcgg
    11701 ccttctcggc ttcggccgtt tcgataaagg agacgagctt cacgtcggcc cccaggtcga
    11761 cgacttcgaa gccgtgggcc gaaagcatgg ccgcgaccag gtttttcccg atgtcgtgga
    11821 tatccccctc gacggttccg atcacgattt tcccggcgct ccgcgccgtt ccgccgccct
    11881 tctccagggc gggccggaga acgctcatgg ccgccttcat cgcctcggcc gaggcgatca
    11941 gctcaggcag gaaatattcc cccgactccc agagctcccc gactttgcgg atgccgggga
    12001 gaaagccttt ttcgacggct tcgagggccg gcgtcccgcc ggcgagggcg gaacgggcca
    12061 gctccccggc cgtctccggc aagccgtcga cgacggacat ggccatcacg gaaaaaatgt
    12121 ctttggtcat gagtcctcct tccgcggcgc ctggggagcc tcgtccggag gccagggctt
    12181 gaacggaagc tccttgatga agatattttg aaacgcctcg ttttgagcca gcggcagatg
    12241 ccggatgacg cggggaaggc tttcagcccg gaggcgctcc cggctcgaaa gcagcagggc
    12301 ccgggctcct tccagggacg tatttccgac gaatttcacc cggtcgccgg gcaggcgggg
    12361 cagaagcccg acggccgcgg cgttttccac atcaagacgg gccccgaagg ccccggccac
    12421 gagaatctcg ttcaggtcct cgatgttcaa ggcggcggct tccaagagca ttcggatccc
    12481 ggttttgacc gcggccgcgg cgagttggaa ttcccggatg tccgaagccg tcaagaacag
    12541 gtcaggcgcc gccgccaggc gtttctccag ccggagaatc cgtccgtcgg gggctaggtc
    12601 gccgcgccgg aggaaggcgg cgatcaagtc gaccaacccg gtgccgcaga caccccgggc
    12661 cggaccgccg ccgatcgtct cgaggacgat cgtcccgtcg tcccgcagga cggcccgctc
    12721 gacggctccg tcctcggccg gaagcccgca cgagatggaa gctgcctcga aagcggggcc
    12781 ggccgccgtc gacgtcgaca tcatcgtccg gtccgtcttg aggaccagtt cgccgttggt
    12841 ccccaagtcg acgaaaagaa cgtttcccgg ccgggcggcc aggtcggtcg ccacgaggcc
    12901 ggcggcgaca tccccgccga cgaagctctg caagttgggc gagaggtaaa cccggccgag
    12961 gggattgatt ttcaagccgg ttcggtcggc cggcagggga ggggccgccg tgaagactcc
    13021 ggcgaacggg gccacggcca ggccgtcgac tggaagaccg agaaacaaat ggctcatggc
    13081 cgtgttgccg gccacgaccg tttcataaat ctcgccggga tccacgcctg attcccggct
    13141 cagctccagt atcaagccgt tgatcgaggc caggacggcg gcccgcaggg ccgcggcccg
    13201 ggacatgtcc ttgaacgccg cggtgatccg gctgacgacg tcggctccga agacggattg
    13261 accgttcaat cctccggccg tcttgatgac cgtccccgtc ccgagatcga cgagatcggc
    13321 ggccacggtc gtcgttccca agtcgacggc caacccgaaa aggcgcgatg acgtatttcc
    13381 ccgctcaacg ccccggagct catcgccggc gcccagaacg gcggtcaccg gggaatcgga
    13441 ctccagagcc tcggaaagaa cgccggccgc ttcaaacaag gcttcgggga caacgcgaag
    13501 agcggaaagg ccgagcccgg ccttcagtcc gtcaaggagg gcacgcggcg attgaagatc
    13561 cggccggacg gaccggacgg tgacttttcg gaccatcgga tcgagcggaa tttccctgcc
    13621 ccggccccgc gtcagaatcc gggccctggg gatcaaggcc tgttccggaa cgcggacgac
    13681 caggtctccc tggacgcgca gccggcaggc caagcggtgg cgtcccaggc cgaggttttt
    13741 ctcccgggcc aggtcccgtt cgctttcatc gccggggggg acttcgccgg agaggatctc
    13801 gacaaagcat ttgccgcaga ctccacggcg cccgcaatag aggttgagag ggacgccatc
    13861 gcggataagg acgtcgctga gaagctcgcc gatatcggcg ggcaagcggc ggcccgaggg
    13921 aaaaagcgag acgatcagcc gcatctcaag cctctcccgc cctcggaccg ccggtcccgg
    13981 attgagggcg gcgccgccgt cccttccaga gaaaataggc cgggattccc gttccgatca
    14041 ggcccgcggc cacggccgcc tgcccgggat gctcgacggc cagggcgacg agaatcgctc
    14101 cgttgaccgc ggcgaacgcg agcgggagca ggggataagg ccgcaggcga tacggccgct
    14161 cgaccgcggg ggccctccgg cgcaatacga tgaccgccgc cccggtcgcc gagaaaaaga
    14221 gaacgagtcc gaacaggaca tagtcgatca gcttttgaaa cgtccctgtc aggatcagga
    14281 tcacggcgac cgcggcttcg gcccagagag ccttgtcggg gacgcgcttg acgggatgaa
    14341 ggattcccag ccggcggaag aaaagtccgt cccgggccat ggcataagga acgcgggcgc
    14401 agaagaaaat gttggccgac aagcatccga agatcgtcag ggcgacgacg ccggcgaagg
    14461 ccggggcggc ggttccttgg ggggccgagg cgaacatagc cgccacggcc tcttggccga
    14521 cccgaacgac tccgcgcatg cgttccatgg gaagagccat actataaacg atattcacag
    14581 ccaaataaag aatgaggacc gaggccgtgc ccaaaagaag agaccggggg acgtttcgcc
    14641 ggggatcgcg gatttcctcg gccgcgcagc tggccgcgta ccatccgtca aacgaccaaa
    14701 gggccgtgat gagcgcggct ccgaacgccg accagcccgg ccacgcgccg gcgggcggga
    14761 acaacggttt caggttgacc gcccgcgttt tgataccgta ccaggcgccg atcgcgacaa
    14821 agccgaggag aaggacgacc cggatgatcg tcaagatcga ttggccccgg gcgctttttc
    14881 tcaatccgct tccgttgaat aaggtgagtc cgatcacggc cgcgatggag atcagccggc
    14941 ccgcggtgat ttggatgtcg agggaaaaga gccttccgct catcatcaaa cgcccgctct
    15001 caaagacagg gaaaaccgct ccgaaatatt cagcgaatcc ggttgcgatg gccgcgacgc
    15061 ccgccccgcc gatgagaaag aaaaatgccc agccgtagag gaacgcggga gccgccccat
    15121 aggcttcctt caagtatacg tacggtcctc cggccctggg aaacatcgaa ccgagttctc
    15181 cgtagcacaa ggcgccgatc atggtgacga ttcccgccgc gagccaggcc gcgagcatcg
    15241 gcatcgaata agggaggaat tcggcgatta agcccgaagt catgaagatt ccggatccga
    15301 tcacggaacc gacgactagg gaagaggctt caaaaagccc catggattca ttttttttaa
    15361 taattatcat aagttattga ttataatata attacaagga tattttccat ttcatcattt
    15421 tgggagcatc atattccagc tgaatgtgtt tttcaatcgt ctttttttaa ttatgatttt
    15481 ctttttcttc aattccactt tctctttccc tcgtatgttc gaagcggtat acaaatctcg
    15541 ttttcaaata atgttgaatc tttttctatt tttaaaatac atagtttttc cctttcatga
    15601 cttagacaaa ggctcggctt ctcaaaaaag cggcttattg tttctcgatc tttttcgagc
    15661 cggagattag gctatttttc ttttgctcga tattatatgt ttataaaaaa taattttaac
    15721 gcatctatgc gcctagattt ttgcatgatg tttctataaa ccccagtccg tcttttttcg
    15781 aaaatgtctt tcttaaaatc gcctagctgg tttctaagat tttattcttc aatatatttt
    15841 tatcgatata attccgcgta caatgaattc gtccaacttc gcgaactttg attttccaga
    15901 tcctcgtttt tttatattta tttttcattt caagcgaata ggggagaagc acttttatta
    15961 gctcatattg atcttcgcga ttttaaaaat ccttttattt ccttttttat gaatcgatta
    16021 cataaaatgt cggccggccg acatataaga gaatcttttt attgaaatat ttccgatttt
    16081 gtttatgata gtataaatta aacgcgagga tatcgttttg atcatagcgg taactaatca
    16141 aaaaggcggc gttggaaaaa ctacgaccgc ggttaatgta tccgcggctt tggcttttaa
    16201 aggcaaaagg gttcttctcg tcgacacgga ttctcaaggc cacagcacga aatccatcgt
    16261 cgtcgagtcc gaacgatttg agcactcgct ctatga
//
LOCUS       DAOOXH010000054        16258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_3505_length_16258_cov_8.694007, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000054 DAOOXH010000000
VERSION     DAOOXH010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 16258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16258
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3505_length_16258_cov_8.694007"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PKV64_07390"
     CDS             complement(<1..240)
                     /locus_tag="PKV64_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HPN16938.1"
                     /translation="MTHAHPFHTDELVRLRRIEGQVRGVQKMIEERRYCVDIETLLPA
                     APLEAKAYLAKVNALNAKGMTPLSQAVIHAADALKA"
     gene            642..1316
                     /locus_tag="PKV64_07395"
     CDS             642..1316
                     /locus_tag="PKV64_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPN16939.1"
                     /translation="MPPFKRIVAVNRPHHITNRGNRRQTIFFSDEDRKVYLGLLGWHA
                     DKYGLKFWAYCLMHNHIHLIAVPENPESMSLTIRETHRKYSSYINILMNWKGTLWQRR
                     FYSFPLEDAHLYRALRYVENNPVRAGIVKKAEEYRWSSAPAHVFGRRDGLLSPNGLGI
                     KRNNWSAYLREQEEEAEIKDIRARSLTGRPLGGDEFIAELEQSLDLELRHKKPGRKKS
                     DANGKP"
     gene            complement(1464..2612)
                     /gene="mnmA"
                     /locus_tag="PKV64_07400"
     CDS             complement(1464..2612)
                     /gene="mnmA"
                     /locus_tag="PKV64_07400"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPN16940.1"
                     /translation="MTEKAASRPEAVVVAMSGGVDSSVAAAVLREQGWKVSGVTLDLF
                     SGREGEGKEEEGCGGKADGRSCGGGAAAREAARVAADLGIPHAVVNLRRRFDRTVVAD
                     FVAEYARGRTPNPCVRCNRFVKFGPLLGRAKRLGAAKIATGHYARVDFDGKSGRWRLL
                     KGVDTGKDQSYFLYALPQEALARTIFPLGGMKKTEVRDLAARFRLHVARKSESQEICF
                     IPGDDYAAFLRGRIPGAFRPGHIVDGKGKIIGCHQGFLNYTIGQRKGMGIAAPRPLYV
                     LALDAAANTIVAGPDEALLKSRFAVAGVNWVAIAGIDGPRAADVKIRSRHEGEPATLF
                     PGPRKSVIVEFARPQRAITPGQAAVFYEGDAVLGGGTISRVLNNSEPV"
     gene            2707..3966
                     /locus_tag="PKV64_07405"
     CDS             2707..3966
                     /locus_tag="PKV64_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16941.1"
                     /translation="MRNTTLRTTAALVRFHAQVGSRVAIRALAPVLASAFGLFYLLRP
                     ELYIMLARRLFAEAEPIARGLLITAALLAAAAAARKSVCFGLGGWLRSLPVGGRAHRR
                     AAVFAITLAETPITFLLFASFFVGTRDLRPPYLLEAAGLLLASAAAAAAVVPAKNKFW
                     RVVIGAAAAVLAGSGNPAYFGISIPVFLAVDYLGGPFEKIRAGTGRRKWMFPIRFPAL
                     IGVRALGLSGWSGYFPAGAVIGMTALFLNNNVLSDHLREAAMRTGGALSAAVFLAFTA
                     GFLVAVRAPWPWSRSWPRSARHRILSDALFLGLPALLLAVPLQWIGWAAVWGAASYLP
                     AGATRAAGAVNAPRESRSKILGTLLLEGGLAAVVYGLWTEAGIAFLFLTPLLVETAAR
                     REQTLKVTAWDEMHHDAVGDPGSWSSI"
     gene            3963..4655
                     /locus_tag="PKV64_07410"
     CDS             3963..4655
                     /locus_tag="PKV64_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN16942.1"
                     /translation="MIRFENIFFGYEKNVPVFQELNLDLDAGLTLLLGPNGSGKTTLL
                     KMAAGVEKPDRGTVRVDGKDLWKNEVAARREIAYLPEHPDLTPYASIREIVRFVCRLR
                     GEPVPRGEEALATLDMDRVAGRSVRELSQGQRRRAVLAAAMIGRPKHLLLDEPLEAMD
                     RRMRDDIMIWIESRIKDGAAAVIVSHDLDPFSVLATSAVGLKEGRPEPHPELPAEAAE
                     RHKILERLARGL"
     gene            4994..5746
                     /locus_tag="PKV64_07415"
     CDS             4994..5746
                     /locus_tag="PKV64_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPN16943.1"
                     /translation="MSGHSKWASIKHKKGAADAKRGKVFTKIIREIAVAARAGGGEPD
                     KNPRLRKAIQDAKNANMPADNIKRAIQKGTGQLEGVSYEEAIFEGYGPGGVAIYLTVL
                     TDNRNRTVSEIRHIFTKNGGQIGTQGCVAFRFKRGGTIDIEKAAVAEEKLMEIALNAG
                     AEDITDEGDMWEVITSPDKFDAVLEAIKKEGVEIVEATNGYIPEDYTKVEGKQAHQTL
                     KLVEELEDHDDVQTVASNFDISPEDLASYESS"
     gene            5752..6270
                     /gene="ruvC"
                     /locus_tag="PKV64_07420"
     CDS             5752..6270
                     /gene="ruvC"
                     /locus_tag="PKV64_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPN16944.1"
                     /translation="MPGHKPMRILGIDPSSVATGYGIVQADGNDSQVLAYGAIKPSRK
                     AALPIRLLEIKTGLERLISAYNPEEIAIENPFFGLNIKTAITLGQVRGAVLVAVASAS
                     LPLFEYSPREIKKAVTGYGQAEKTQVGIMIRALLRIDDDAMEEDASDALACAFCHLNT
                     RLAKKNLEEMKI"
     gene            complement(6501..7583)
                     /gene="ychF"
                     /locus_tag="PKV64_07425"
     CDS             complement(6501..7583)
                     /gene="ychF"
                     /locus_tag="PKV64_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPN16945.1"
                     /translation="MKICLVGPPLSGKTVLFAALTGMAPAEAAAHGAEPVAVVKVPDD
                     RVDRLAEIYKPKKKIQATIEFSELAGVAAGDGRKTGFSEQFLGKLRTADALLLVVRAF
                     GDPAVPHPRESVDPVRDLRDAETEFLLSDLAVIEKRFDRLEKEIAAKKSDRDVRELAV
                     LKTCRAHLEAERPLRLLEFKPDEELLIRGYRFLTQKPLIVAVNLDEEDIRREGEVLAP
                     FAGWAEQANTAVIGLSARIEMELRELPDAELKAFLADYGITRPAREKLIAATYRLMGL
                     LSFFTVGQDEVKAWTIRDGTKALAAAGEIHSDIERGFIRAEVVPFSEFIAAGSMAECR
                     TRGTLRLEGKEYQVQDGDIINFRFAV"
     gene            complement(7895..8500)
                     /locus_tag="PKV64_07430"
     CDS             complement(7895..8500)
                     /locus_tag="PKV64_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HPN16946.1"
                     /translation="MKDIIIILAALGFFAVTVGAAEAAASEDGPGTARGSEAKTAGTA
                     GVPALETVAYVDLQRYMGTWYEIAAFPQKFQKGCHCSKAEYEMTDKGYVRVVNSCRKD
                     SAEGEVKVARGKAFVVEGTGNAKLRVQFFWPFRGDYWILELPEDYSHVVVGAPNRDYL
                     WILARTPSIDESLYQQIIGRCEEMGFDATRLVRTDQGCAGR"
     gene            8583..9017
                     /locus_tag="PKV64_07435"
     CDS             8583..9017
                     /locus_tag="PKV64_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HPN16947.1"
                     /translation="MKILFVCYANLCRSPLAEAISESEFSGRIEARSAGVAPAPGRPF
                     EEAVDVARRFYKRNISRHRTRHVLDFSLQDFDYIIGMDSNVFMQLTAMSEIPKEKLYG
                     WEIPDPAGLGVETYEKAARLIEREIEHFLEFREREAYEAGSS"
     gene            9113..10387
                     /locus_tag="PKV64_07440"
     CDS             9113..10387
                     /locus_tag="PKV64_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016865923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HPN16948.1"
                     /translation="MKEENAMALDLERVRHQFPALAGNAVFLDNPGGTQVVKNCLDRM
                     LDYLIEANANHDGAFKTSRESDALAARARQAAADFLNARPEEVVFGANMTTLTFHLSR
                     SLAPRLGPGDSLVVTRLDHDANISPWLLLARDRGAEILWVDFHPEDGTLDLADLDRAL
                     EKKPKLIAVGYASNALGTINPVADIAARARAAGALVFVDAVHYAPHGPIDAAALGCDF
                     LACSAYKFFGPHLGLLYGRRELLEELPAYKVRPADDRVPFRFETGTPNFEALAGLLGT
                     IEYLEELGTRFGEDPEAEPADDPSGRKRKLRRAAAAVRAHERTLAAAMFETLQAIPSM
                     KIFGLTDPARFDERVATFSFRLGDVPPRRIAEELDRDGIYAWDGHYYALETVRRLGLE
                     DKGGMLRVGAVHYNTLEDIARLGRSLARIAKA"
     gene            complement(11533..12099)
                     /locus_tag="PKV64_07445"
     CDS             complement(11533..12099)
                     /locus_tag="PKV64_07445"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HPN16949.1"
                     /translation="MEFLNFDLFKWVILPLLIFTARIFDVSLQTMRIIFISKGRRLLA
                     PLLGFFEVIIWLVAITQIMQNLTNPFYYLAYGAGFAMGTYVGMRIEEKLAVGNVLLRV
                     ITPNNADALVERLREEKHGVTCIDGEGKSGKVKIIFLVLDRVNLPRVIETVRQCRPEA
                     FYSVEDIRAVGRGYFRVKTRDRLWSQRK"
     gene            complement(12114..>12409)
                     /locus_tag="PKV64_07450"
     CDS             complement(12114..>12409)
                     /locus_tag="PKV64_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16950.1"
                     /translation="AAETTPFRPPAEPRIAHDPCFSIRSAADLAGTPLPAPKEKFSDG
                     CLLAVDVISPEAHTGKKTVFQARPVVGGVLVKRLDDPALRAKWSAAAGTRPAE"
     assembly_gap    12410..12509
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12712..13707)
                     /locus_tag="PKV64_07455"
     CDS             complement(12712..13707)
                     /locus_tag="PKV64_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPN16951.1"
                     /translation="MESSFGFIAFIIAIALIFDFTNGLHDAANSVATIVSTRVLSPKQ
                     AVIWAAFFNFVAFLIFGTAVAKTIGTGLIDISVVDPMVILCGLLGAVSWNILTWYWGL
                     PSSSSHSLMGGYLGAAVAKAGFKVVIVSGWVKIAVFIVLAPLLGMIFGIVLKVVTTWI
                     VHKRPMAVVDVWAKRLQFASAALYSLGHGGNDAQKTMGLIAGLMFTSGHMKEFHIPLW
                     IVLSAHTAIALGTLSGGWRIVKTMGQRIVKLKPIDGFCAETASAVSIFTATHMGVPVS
                     TTHVITGAVAGVGAAKNAAAVKWQVTIKIVWAWILTIPMSALCAGLLYEFFGILF"
     gene            complement(13713..14339)
                     /locus_tag="PKV64_07460"
     CDS             complement(13713..14339)
                     /locus_tag="PKV64_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPN16952.1"
                     /translation="MKFLLPKAPAFAEHFEEMSVCLAEIADVFHTFSVEFRDFEKYWQ
                     KSKEIEHRADDITHGIINLINKSFITPFDREDIYRLIHEYDDIIDLLENTLHNVYLYE
                     VTEKRPFIDEFARLIGRATAALIALVKETFDKQKYTDEIWKEIREIHDLEDEGDVIYH
                     RTLRQLFKEEKDPIELMKWKDILITLERIMDVFQYMSNTVEGIVVKGS"
     gene            14820..>16258
                     /locus_tag="PKV64_07465"
     CDS             14820..>16258
                     /locus_tag="PKV64_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPN16953.1"
                     /translation="MSANDGYPPAGGASEAAREKSAAAKSSVIAAVFLTGMKIVVGLA
                     TGSLGILAEAAHSALDLVAAAMTLIAVRVSSRPADQTHTYGHGKIENLSAMLETALLL
                     ITCVWIVIEAVRRLFFQDVPVEASLWAFLVMAVSIVIDLSRSRVLARAAKKHHSQALE
                     ADALHFSTDVWSSSVVILGLLLVKLSGLLDVPWLAKADAVAALGVSAIVVLVSLKLGK
                     KTITDLLDGVPADLREAVVRAAHVPGVIEVDRARIRRSGPEIFVDVVLFVSRHAAFEH
                     AHDISNEAKNSIRRALGLPDADIIVHVAPSRDETRDMVEHIRDIAARLDLAAHEIRFF
                     EADGRPALDLHLEIRGAANVADAHARASAFEEAVHAAVPGLDRIFTHLEPVGGQAFIA
                     PLPPGEDRAIRRRIAELSEEMGIVCDPHHILIEETVGEVCVSFHCSVDPATPLAEAHK
                     FSERFEMALRRDIPRVGRVLIHIEPPEPAG"
BASE COUNT         3243 a         4999 c         4627 g         3289 t
ORIGIN      
        1 ggccttgagc gcgtcggcgg catggatcac cgcctgcgac agcggcgtca tgcccttcgc
       61 gttcagcgca ttcaccttgg ccagatacgc cttcgcctcg agcggcgcag ccggcagcag
      121 ggtctcgata tcgacgcagt agcggcgttc ctcgatcatc ttctggacgc cccggacctg
      181 gccttcgatg cggcgcagcc ggaccagttc gtcggtatgg aacgggtgag cgtgggtcat
      241 gtctccaagc ctccacttca taatatatat acccccaggg ggtatgtcaa gccgatcccc
      301 tctgaaatca gaacctgttt atccatttcc ccatttccag ccattaaatc ggtacctgtt
      361 taaccatttc cttattttcc ccggcttatt gaatcggtca atcggtacct gtttatccat
      421 ttcccctttc caccggatca ggcggaaaac acggaaatgg ttgaccaggt gcaaatttca
      481 ggtgctattt tcggcctcgc tattctcgtc tttccccggc ttattaaatc agtacctgtt
      541 taaccatttc ctcattgccg gccggatttc ccccgaaatg agaatatttc aaatcgcggg
      601 acgtcttttt cattgaatcc cgcgattccc ggagaccaaa gatgcctccc tttaagcgca
      661 ttgtcgccgt caaccgcccg caccatatca ccaaccgggg aaaccgccgg caaaccattt
      721 ttttcagcga tgaggacagg aaggtctacc tcggccttct tggctggcat gcggacaagt
      781 acggactcaa attttgggcc tattgcctga tgcacaacca tattcatctt atcgcggtgc
      841 cggaaaatcc cgagagcatg tctctgacca ttcgggagac ccatcggaaa tattcctcct
      901 acatcaatat tctcatgaat tggaaaggca cgctttggca gaggcgtttt tattcgtttc
      961 ctctagaaga cgcccatttg taccgggcgc ttcgttatgt cgagaataat ccggttcggg
     1021 ccgggatcgt taaaaaagcg gaggaatacc gctggtcaag cgcgccggcc catgtgttcg
     1081 gccgtcgaga cggcttgctc tcccccaacg ggctcggcat caaaaggaat aactggagcg
     1141 cgtacctcag agaacaggag gaagaagccg aaattaagga tatccgcgcc cggtcgttaa
     1201 ccggccggcc cctgggcgga gatgaattca tcgccgagct cgagcaatcc ctggatcttg
     1261 agctccggca taaaaagccg ggccggaaaa aatctgacgc gaacggaaag ccttaaagga
     1321 aatcatccct tatccattcg gcacctgtca ttcggtacct gtttatccat ttcccctttc
     1381 caccgaatca gacgggaaaa cacggaaatg gttgaccagg tacggatttg gttagaattt
     1441 atgtccagga aacagggaaa tggttaaaca ggttccgaat tattgaggac ccgggaaatg
     1501 gtcccgccgc ccaaaacggc gtctccctca tagaaaacgg ccgcctgccc gggagtgatc
     1561 gccctctgcg gtcgggcgaa ctcgacgatc acgcttttcc tcggtccggg gaaaagcgtc
     1621 gccggctctc cctcgtgccg cgaacggatt ttgacatccg ccgcccgcgg tccgtcgatc
     1681 cccgcgatcg caacccagtt gacgccggcg acggcgaaac gcgatttgag aagagcctcg
     1741 tccggcccgg cgacgattgt gttggccgct gcgtccagcg caaggacata aagcgggcgc
     1801 ggagcggcga tgcccatccc cttccgctgg ccgatcgtgt aattcaggaa tccctggtgg
     1861 catccgatga tcttgccctt tccgtcgacg atatgtcccg gccggaatgc accgggaatc
     1921 cgtccgcgca gaaacgcggc gtagtcgtcg cctggaatga aacagatttc ctggctttcg
     1981 gatttccggg cgacgtgcag tctgaaacgc gcggccaaat cccggacttc ggtttttttc
     2041 atcccgccca gcgggaaaat cgtccgggcc agcgcctcct ggggcagggc ataaaggaaa
     2101 tacgactggt ccttgccggt atcgactccc ttaagaaggc gccaacggcc gcttttcccg
     2161 tcgaagtcga cacgagcgta atggccggtc gcgatcttcg ccgctccgag ccgtttcgcc
     2221 cggccgagca atgggccgaa cttgacgaac cggttgcacc tcacacaggg attgggcgtc
     2281 cgtccccggg catactcggc gacgaaatcc gcgaccactg tccggtcgaa ccgccgccgg
     2341 agattcacga cggcgtgggg aatgcccaag tcggccgcta cccgggcggc ttcccgggcg
     2401 gccgcgccgc cgccgcagct ccggccgtcg gctttcccgc cgcatccctc ctcctctttc
     2461 ccctcccctt cccgtccgga gaaaagatcc agcgtgacgc cggaaacctt ccagccctgc
     2521 tccctcaaga cggccgcggc cacggaggaa tcgaccccgc ctgacatggc gacgacgacg
     2581 gcttcagggc ggcttgcggc tttttcggtc atcggtggtt atgttatatt atgttatatt
     2641 agaaggcggc ggtcatttta acactctttc cgaccgggct ccaggccgct cgactcgacg
     2701 gaaatgatgc gaaatacgac gctgcggacg acggcggccc tcgtaagatt ccatgcccag
     2761 gtcggctccc gcgtcgccat ccgggctctc gcccccgttc tcgcctcagc cttcggcctc
     2821 ttttatctcc tccgccccga gctctacatc atgcttgccc gccgtctttt cgccgaggcc
     2881 gagccgatcg cccgcggtct tctcataacc gccgcgcttt tggccgcggc cgccgcagcc
     2941 cgaaaatccg tttgtttcgg gctgggcgga tggctccgca gcctccccgt cggcgggagg
     3001 gctcatcggc gggcggccgt cttcgccatc accctggccg aaacgccgat caccttttta
     3061 ctgttcgcct ccttctttgt cgggacgcgg gatcttcgcc ctccttatct cctcgaggcg
     3121 gccgggctct tgctggcttc cgcggccgcg gcggccgcgg tcgtcccggc gaaaaacaag
     3181 ttttggcggg tcgtcatcgg ggcagcagcc gccgtcttgg cgggttcagg aaatccggcc
     3241 tattttggga tctcgatccc cgtttttctg gctgtcgatt atctcggcgg tccgttcgaa
     3301 aaaatccgag ccgggacggg ccggcggaaa tggatgttcc cgattcgttt cccggcgttg
     3361 atcggcgtgc gggcgctcgg actctccggc tggtcgggat attttccggc cggggcggtc
     3421 atcggaatga ccgctctctt ccttaataac aacgttctgt ccgatcatct ccgggaagcg
     3481 gccatgcgga cgggaggggc gctgagcgcg gcggttttcc tggcgtttac agccggattt
     3541 ctcgtcgccg tccgcgcgcc ctggccatgg agccggtctt ggccgcgctc ggcccgacac
     3601 cggattctat ccgacgctct ctttctcggg ctcccggcgt tacttctggc tgtccccctg
     3661 cagtggatcg gctgggcggc agtctgggga gccgcatcgt atctgccggc gggagcgaca
     3721 agagcggccg gggcggtcaa cgcgccccgg gaaagccggt cgaaaatatt ggggacgctc
     3781 ttgctggaag gcgggttggc cgcggtcgtt tatggactgt ggaccgaagc cggcattgct
     3841 ttcctttttc ttacgccgct cctcgtcgag accgccgccc ggcgggagca gacgctgaaa
     3901 gtcacagcct gggatgagat gcatcacgac gccgtcggcg atcccggctc ttggagctcg
     3961 atatgattcg attcgaaaac atcttcttcg gctacgagaa aaacgttccg gtttttcagg
     4021 agttgaatct cgacctggac gcggggctga cgctgctttt gggaccgaac gggtcgggca
     4081 agacgaccct tctaaagatg gccgcgggcg tggaaaagcc cgatcggggc acggtccggg
     4141 tcgacggaaa agatctttgg aaaaacgaag tcgcagcccg aagggaaatc gcctacctgc
     4201 ccgagcatcc cgatctaacg ccctatgcct ccatccggga gatcgtccgg ttcgtctgcc
     4261 ggctgcgcgg cgaaccggtc ccccgcggag aagaagccct ggcgacgctg gacatggacc
     4321 gcgtggccgg ccgctcagtc cgggagcttt cgcagggaca gcggcgccgg gccgtgctgg
     4381 ccgcggcgat gatcggccgg ccgaagcacc tcctgttgga cgagccgctg gaagcgatgg
     4441 accggcggat gcgggacgac atcatgattt ggatcgaaag ccggatcaag gacggggcgg
     4501 cggccgtcat cgtttcccac gaccttgatc cgttttccgt tctggcgacc tcggcggttg
     4561 ggttgaagga aggccggccc gaaccacatc ccgagcttcc ggctgaagcg gcggaacggc
     4621 acaaaatcct cgagcggctt gcccgcggtc tttgatattg aatttaggga cgccctctcc
     4681 gtcatcatta atcggtacct ggttaactat ttccgtattt cttctccccc gaactcatga
     4741 gacgggaaaa agcaatcttg ataaggaatt atcatgagtt gtttccagat ggaatcgaga
     4801 aaatggttaa ccaggctgga atttggaatt tgcccacgga ggaagccggt ggaaacaggg
     4861 aaatggttaa acaggttccg atttgccggc tccaggttga tttacttgat gaaaaaggga
     4921 agatgggtct tgtgtcacga aaaagcattt gctaaaatga tctgcgtatt cttatccttc
     4981 aaggagctcc acaatgtccg gtcattctaa atgggcgtcc atcaaacata aaaaaggcgc
     5041 cgccgacgcc aagcgcggca aagtcttcac gaaaatcatc cgggaaatcg ccgttgccgc
     5101 cagggccggc gggggcgaac cggacaaaaa tccgcgtctg cgaaaggcga tccaggacgc
     5161 caagaacgcc aacatgcccg ccgacaacat caagcgggcc atccagaagg gaaccggaca
     5221 attggaaggc gtgtcctacg aggaagccat attcgaaggg tacggaccgg gcggcgtggc
     5281 tatttatctc actgtgctga ccgacaaccg caaccgaacc gtttccgaaa tccgccacat
     5341 cttcacaaaa aacggcggcc agatcggaac ccaaggctgc gtcgccttcc gtttcaaacg
     5401 gggcggcacg atcgacatcg aaaaagccgc ggtcgccgaa gagaagctca tggaaatcgc
     5461 cttgaacgcc ggagcggaag acatcaccga cgaaggcgac atgtgggaag tcatcacttc
     5521 ccccgacaag ttcgacgcgg tattggaggc gatcaagaaa gaaggagtcg agatcgtcga
     5581 ggcgaccaac ggctacatcc ccgaggacta caccaaggtc gagggcaagc aggcccacca
     5641 aaccctcaag ctcgtcgagg agctcgagga ccacgatgat gtccagaccg tcgcgtccaa
     5701 cttcgacatc tcgccggagg atctcgcctc gtacgaaagc tcctgatttt cgtgccgggc
     5761 cacaagccca tgcggatact cgggatcgat cccagcagcg tcgcgaccgg atacgggatc
     5821 gttcaagccg acggaaacga ttcccaggtt ctcgcttacg gcgcgatcaa gccgtcccgc
     5881 aaagccgccc ttcccatccg gctcctcgaa attaaaaccg ggcttgaacg cctgatctca
     5941 gcctacaatc cagaagaaat cgcgatcgaa aatccttttt tcggcctcaa tatcaagaca
     6001 gcgatcactt tgggccaagt ccgcggggcg gttttggtcg ccgtcgcctc ggcttccctc
     6061 cccctcttcg aatattcccc tcgcgagatc aagaaggccg tgaccggata cggtcaggcg
     6121 gaaaaaaccc aagtcgggat catgattcgg gcccttctcc ggatcgacga tgacgccatg
     6181 gaggaggatg cctccgacgc cctcgcctgc gccttctgcc acctcaatac gcggctggcg
     6241 aaaaagaacc tggaggagat gaaaatctga cggccgcggc cgcggccggc ctcatttcgg
     6301 cgcctggtcc gccttttccc tttttccgga ggagatattc agtacctggt tatccatttc
     6361 cttattatca gccttcatcc gcctggaaaa aatccttgat aggattacat atttatcgtc
     6421 agatgcaccg gacggaaaga aatgcggaaa tggttgaaca ggtaccgatt ggaaaaggca
     6481 ccgattgatc aacactccgg ttagaccgcg aaccggaaat tgatgatatc cccgtcctgg
     6541 acctgatact ccttgccctc cagccggagc gtcccccgcg tccggcattc ggccatcgat
     6601 ccggccgcga tgaattcgct gaaaggcacg acctccgcac ggatgaagcc gcgctcgatg
     6661 tcggaatgga tctcccccgc cgcggccagg gccttcgtcc cgtcccggat cgtccaagcc
     6721 ttgacttcgt cctgtccaac cgtgaaaaac gacaacagcc ccatcagccg gtaagtggcg
     6781 gcgatgagct tctcccgggc cggccgcgtg atcccgtaat ccgccaaaaa ggctttgagc
     6841 tccgcgtccg gcaactcgcg caattccatc tcgatccggg ccgaaagccc gatgacggct
     6901 gtattcgcct gctccgccca gccggcaaac ggggcgagaa cctcgccttc acggcggatg
     6961 tcttcttcgt ccagattgac ggccacgatc agcggcttct gcgtcaagaa tcggtacccg
     7021 cggatcagga gctcctcgtc cggcttgaac tcgaggagcc ggagcggacg ctccgcctcc
     7081 aggtgggccc ggcaggtttt cagcacggcc agctcgcgga catcccgatc gctttttttg
     7141 gccgcaatct ccttttccag acgatcgaag cgtttctcga tgacggccaa atcgctcagg
     7201 agaaactccg tttccgcgtc ccgaagatcg cggaccggat cgacggattc cctcggatgg
     7261 ggaaccgccg ggtctccaaa ggctcggaca accagaagca gcgcgtcggc ggtccggagt
     7321 ttccccaaga actgctcgct gaagccggtt ttacggccgt cccccgccgc cactccggcc
     7381 aactcggaga attcgatcgt cgcctgaatt tttttcttcg gcttgtagat ctcggccaag
     7441 cggtcgaccc ggtcgtccgg aaccttgact acggccacgg gctcggcccc gtgagccgcg
     7501 gcctccgccg gcgccatccc ggtcaaggcg gcgaaaagaa cggtcttgcc ggaaagaggc
     7561 ggaccaacaa ggcagatttt cacaatgatc tctctttaaa atgaatccgt tcgactaaag
     7621 ttcagaatac gtttttcccc ggtttttttc aagaaatgaa tcggcacctg ttcaaccatt
     7681 ttcctattcc cattcatctt cgaatgagag tatttcggga aaagggccaa tatggccgcc
     7741 ggcgccgcag gcaaaagcaa atccggaaat ggataaccag gtaccgattt cgagatttcg
     7801 aaatgaggaa atggataaac aggtaccgat ttaacgggga ccgatttcgc cggaaatatc
     7861 gcgggaaaaa cgatttcggg gggaaaggcc tccgctagcg cccggcgcag ccctgatcgg
     7921 tcctcaccag ccgggtcgcg tcgaatccca tctcctcgca gcggccgatg atctgctggt
     7981 acaagctctc gtcgatcgac ggcgtgcggg ccaggatcca caggtaatcg cggttggggg
     8041 ccccgacgac gacatggctg tagtcctcgg gcaattccag aatccagtaa tccccgcgga
     8101 aaggccagaa aaattgaacg cggagcttgg cattgcccgt tccctccacg acaaaggctt
     8161 tccccctggc gactttgacc tcgccctcgg ccgaatcctt ccggcaggaa ttgacgaccc
     8221 ggacataccc cttgtccgtc atctcatatt cggccttgga acaatggcac cccttctgaa
     8281 acttttgggg aaaggcggcg atctcgtacc atgtccccat atacctttgc aggtcgacat
     8341 aggcgaccgt ttccagggcg ggaacaccgg ccgttcccgc cgttttcgct tcactccctc
     8401 tcgccgtccc gggcccgtct tccgaagccg cggcctccgc cgcgcccacg gtaacggcga
     8461 aaaaccccaa agcagctaga ataatgatga tatccttcat gttgttatta tcctcccgat
     8521 tccgcccgcg tttcaagcgt tattgacagg cccggccgct ccggctataa ctcggaagga
     8581 taatgaaaat cctcttcgtc tgttacgcaa acctgtgccg gagccccctg gccgaggcga
     8641 tctccgagtc cgagttctcc ggccggatcg aagcccggag cgccggcgtc gcccccgccc
     8701 ccggccgtcc tttcgaggag gcggtcgatg tcgcccgaag gttctacaag cggaacatct
     8761 cccgccaccg gacgcgccat gtcctcgact tttcgctcca ggacttcgac tacattatcg
     8821 gcatggattc gaacgtcttc atgcaattga cggcgatgtc cgaaattccg aaggagaagc
     8881 tctacggttg ggaaatcccc gacccggccg gcctcggcgt ggaaacctac gagaaagccg
     8941 cccgcttgat cgaacgggag atcgaacatt ttctcgagtt ccgggagcgg gaagcctacg
     9001 aagccggctc ttcctgagcc ccccgacggc ctgatggcag aagatcaagc cgccggcggc
     9061 caaagagaac agagaacgac gaacaaaacg cggtgatcat aacaagagaa aaatgaaaga
     9121 ggagaacgcc atggccctgg acctcgaacg cgtccgtcat caattccccg ccctcgccgg
     9181 caacgccgtt tttctggaca accccggggg cacccaggtc gtcaagaact gtctcgaccg
     9241 gatgctcgac tatctcatcg aggccaacgc caaccacgac ggcgccttca aaacgagccg
     9301 cgagtcggac gccctggccg cccgcgcccg ccaggccgcg gccgattttc tcaacgcccg
     9361 ccccgaagaa gtcgtctttg gggcgaacat gacgaccctg acctttcatc tttcgcgctc
     9421 cctcgctccg cgcctcggcc ccggcgattc cctcgtcgtc acccgtcttg accacgacgc
     9481 caacatttcg ccctggcttc tcctggcccg cgaccggggg gccgaaatcc tctgggtcga
     9541 tttccatccc gaagacggca ctctcgactt ggcggatctc gaccgggcct tggagaaaaa
     9601 acccaagctc atcgccgtcg gctacgccag caacgccctc gggacgatca atcccgtggc
     9661 cgatatcgcc gcccgggccc gggcggccgg ggcgcttgtc ttcgtcgacg ccgtccatta
     9721 cgccccccac gggccgatcg acgcggccgc cctcggctgc gacttcctgg cctgctcggc
     9781 gtacaagttc ttcggtcccc atctcggcct tctctatggg cggcgggaac tcctggagga
     9841 gcttccggcc tataaagtcc ggccggccga cgacagggtc cctttccgat tcgagacggg
     9901 gacacccaac ttcgaggcgc tcgccggcct gctcgggacg atcgagtatc tcgaggagct
     9961 cggaacacgc ttcggcgagg atcccgaggc cgagccggcc gacgatccgt ccggacggaa
    10021 gcggaagctc cgccgggcgg cggcggccgt ccgggcccat gagcggacgc tggccgcggc
    10081 catgttcgaa acgctccaag cgatcccctc gatgaaaatc ttcgggctca cggacccggc
    10141 ccgcttcgac gaacgcgtcg cgaccttctc attccgcctg ggggacgtcc ccccgcggcg
    10201 gatcgccgag gagctggacc gcgacgggat ctatgcctgg gacgggcact actatgccct
    10261 cgagacggtt cggcgcctcg gcctcgagga caagggcggc atgctccggg tcggggccgt
    10321 ccattacaac actctcgaag acatcgcccg gctcggccgg tcgctggccc ggatcgccaa
    10381 ggcctgaatt tttttcggga cgtcggcgga agaatcatca atccgccgcg gatctggtca
    10441 gagagcgatt cgccccggcg gccgaaacgt ccggcgccgg atgcttccgt gcagatgcgg
    10501 tgaacccgga acaggtctcc cggatcaggt cttttcggcc agccggcgaa agcttcgatt
    10561 gagggcgatg aaccacagtg accagccgag gtgcatgatt ctctgcttgg cgccgatcca
    10621 ggggccgaga atatccggca tatacgccgt tcgttgtggt aaaataaatt aaaagaagac
    10681 tatgagaata aaatatttac taatttttgg attgttaagt ccgttggttt tttggattac
    10741 gacaattctt tgcggattac gacttgaagg atataatcat tttacctggt tggtaagtga
    10801 attaggagca ttagggacca ggacacaggt tatttttaca ttcggattag ttttaagttc
    10861 catccttaat tttgttttcg ttattggttc atttaaattt tgtaaaatga ggcaattgaa
    10921 tctgattcct gtgatattcc tattgtttta ttctttcctt gcgggaccag ctctatttcc
    10981 aatgcctttg agactgcatg gtattgttgg aatccctttt cctctgataa tgtttgctcc
    11041 gatcgttagc ctcattttct ggagaaagaa ggagtatctg gtaaaaataa gagtgatagc
    11101 catcattagt ttcctgatta tgctattggg atttttaatt tattttccga atatcctgcc
    11161 tggatatttc ggattaaaac aaagattttt atatgcaggc tggactgttt ggtctgttta
    11221 tttgagcttc aggtttttga aattaagtgc gaaataactc aagtgaactg gaggacatcc
    11281 ctgatctggc atttaccctg cgggctgacc ggggtgtcgg aatgacccct cccgattaat
    11341 attgcgttgg tgtcaggtct tacattaaac atttggaacc caggtggctg tagtcgccca
    11401 gccgggcggc gcagagaaat atcgttcccc aaaacaacag gaccgccagg atgcctgcat
    11461 cgagccatcg gcttttattc atgggggcga tattcccttg aacgttcatt cggaaacaag
    11521 acgagcgaaa tcctatttcc tttggctcca cagccggtcg cgggtcttga cccggaaata
    11581 tccccggccg acggcccgga tgtcctccac cgaatagaac gcctcgggcc ggcattgacg
    11641 gaccgtctcg atgacccggg gcagattgac ccggtcgagg accaagaaga tgatcttgac
    11701 cttcccgctt tttccctctc cgtcgatgca ggtcacgccg tgcttttcct cccggaggcg
    11761 ttcgacgagc gcgtccgcat tgttcggagt gatgacccgg agaaggacgt tccccacggc
    11821 cagtttttcc tcgatccgca tgccgacgta ggtccccatg gcgaagccgg ccccgtaggc
    11881 caggtaataa aaaggattgg tcaggttctg catgatctgg gtgatggcca ccagccagat
    11941 aataacctcg aaaaaaccca gaagcggggc tagaagccgc cggcccttcg agatgaagat
    12001 gatccgcatc gtctggaggc tgacgtcgaa gatgcgggcc gtgaaaatca gcaggggaag
    12061 aatcacccat ttgaagaggt cgaaattcag gaattccatc gccgccttcc tcctcactcc
    12121 gcgggccggg ttccggcggc cgcggaccat tttgcccgga gggccgggtc gtccagcctc
    12181 ttgaccagca cgcccccgac gaccggccgc gcttggaaga ccgtcttttt tcctgtgtgc
    12241 gcttccggag agatgacgtc gacggccaag aggcaaccgt cgctgaattt ctccttgggg
    12301 gccggaagcg gcgttccggc caggtcggcc gccgaccgga tgctgaagca gggatcatgg
    12361 gcgatccgcg gctcggccgg cggccggaag ggcgtcgttt ccgcggccgn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna agagcgggcg taatcaggaa gatgcggcaa
    12541 aagcgggcga tcatgacgtc ctccggggaa catttcaact gagtctattt tatgtttcgc
    12601 gtgagtattt ctcaatcccg gcgcatggcc ggtcaattcg gaacctgttt atccatttcc
    12661 atatttcaac aggttcagat ttaaatcggg aaatggataa acaggttcag attaaaatag
    12721 gatgccgaag aactcgtaca aaaggccggc gcagagggcg ctcatcggga tcgtcaaaat
    12781 ccaagcccag acgatcttga tcgtcacctg ccacttgacc gcggccgcgt tcttggccgc
    12841 tccgacgccg gccacggccc cggtgatgac atgggtcgtc gagacgggaa cccccatgtg
    12901 ggtggctgtg aagatggaga cggcgctggc cgtctcggcg cagaatccgt cgatgggttt
    12961 aagcttgacg atccgctggc ccatcgtctt gacgatccgc catccccccg aaagcgtgcc
    13021 caaggcgatc gccgtgtgag ccgagaggac gatccacaag gggatgtgga attccttcat
    13081 gtgtcccgag gtgaacatca ggccggcgat gagacccatc gtcttctggg cgtcgtttcc
    13141 gccgtggccg aggctataga gagcggccga ggcgaattgc aggcgcttgg cccagacatc
    13201 gacgacggcc atcggccgct tgtgaacgat ccaggtcgtc acgaccttga ggacgatccc
    13261 gaagatcatc ccgagcaaag gggccaggac gataaacacc gcgattttca cccagccgga
    13321 aacgatgacg accttgaagc cggccttggc cacggccgcc ccgagatagc cgcccatcag
    13381 ggagtgggag gagctcgagg gcaatcccca gtaccaggtc aggatgttcc aggagacggc
    13441 tcccagaagc ccgcagagga tgaccatcgg gtcgacgacg gaaatgtcga tcaagccggt
    13501 tccgatggtt ttagccacgg ccgtgccgaa gatcagaaag gccacgaaat tgaagaacgc
    13561 ggcccagatg acggcttgct tggggctgag cacccgcgtt gagacgatcg tcgcgaccga
    13621 gttggccgcg tcgtgcaggc cgttggtgaa atcaaaaatg agggcgatgg cgatgatgaa
    13681 ggcgatgaaa ccgaagctgg actccatcgg agtcagctgc ccttgacgac gatgccctcg
    13741 accgtgttgc tcatgtactg gaagacgtcc atgatccgct ccagggtgat caggatgtcc
    13801 ttccacttca tcagctcgat cgggtccttc tcctctttaa acagctggcg gagggtccgg
    13861 tggtagatca cgtcgccctc gtcctccagg tcatggatct cccggatctc cttccagatc
    13921 tcgtccgtgt acttctgctt gtcgaacgtc tccttgacca gggcgatcaa agcggcggtc
    13981 gccctcccga tcagccgggc gaactcgtcg atgaacgggc gcttctcggt cacttcgtag
    14041 aggtagacgt tatggagggt gttttccaga agatcgatga tgtcgtcgta ctcatggatc
    14101 aggcggtaga tgtcttcccg gtcgaacggg gtgatgaagc tcttgttgat gaggttgatg
    14161 atcccatggg tgatatcatc cgcccggtgt tcgatttcct tggatttctg ccaatatttc
    14221 tcgaaatcgc ggaattcgac ggaaaatgtg tgaaagacat cggcgatttc ggcaaggcag
    14281 accgacattt cctcgaagtg ctcggcgaac gcgggggctt tgggaagaag aaatttcatg
    14341 atgacgcctt gggaaaaatg agaaatataa atgcggcgga gggggattgc gacggaagac
    14401 ggacagccat gagggagtca ctatatccga cgcggaggcg gaaatcaaat caagcgcctc
    14461 gcgccgggac cggcgcattt tccgcccttc ggaacccgcg tcccgcgttc tccgcataga
    14521 agataaggga gggggttatc cggttcaaac gcgaactccg ggaagacggt tgtctcattc
    14581 gaagcggagc ctgcggaaac cgcccgagac cagggatcgg atggatctcg acctccggat
    14641 caacggcttc acggggccgc cggaaattca gcggcaaccg cgcgtccgac gatcctcccg
    14701 ccggagaacg atccccccat cggccgtttt tcgagcctcc cgcgtcctct tttcaatcgt
    14761 gcgcgacacg ggtttccatc cggtcgacag gcccgggacg ttgtgctata taagacccta
    14821 tgtccgcgaa cgacggttat ccccccgccg ggggcgcctc cgaagccgcc cgcgagaaaa
    14881 gcgcggccgc aaaaagctcc gttattgctg ctgtttttct caccggcatg aagatcgtcg
    14941 tcggcctggc caccggctcg ctgggcatcc tggccgaggc ggcccattcg gccttggacc
    15001 tggtcgccgc ggccatgacc ctcatcgccg tccgcgtatc cagccggccg gccgatcaaa
    15061 cccacaccta tggtcacggc aagatcgaaa acctgtcggc catgctggag acggcccttc
    15121 tgctcatcac ctgcgtctgg atcgtcatcg aggccgtccg gcggctcttt ttccaggacg
    15181 ttcccgtcga agcgtcgctg tgggcgttcc tggtcatggc cgtgtcgatc gtcatcgatc
    15241 tgtcccgctc ccgggtcctg gcccgggcgg ccaaaaaaca ccacagccag gccctcgaag
    15301 ccgacgccct tcatttctcg accgacgtct ggtcctcttc cgtcgtcatc ctcggccttc
    15361 tcctggtcaa gctgtccggc ctgctcgacg tcccttggct ggccaaggcc gacgccgtcg
    15421 ccgccctggg agtctcggcc atcgtcgtcc tcgtcagcct gaagctcggc aaaaaaacga
    15481 tcaccgacct tctcgacggc gtgccggccg atctgcgcga ggccgtcgtc cgggcggccc
    15541 atgtccccgg cgtgatcgaa gtcgaccggg cccgcatccg gcgctccggc ccggagatct
    15601 tcgtcgacgt cgttctcttc gtcagccgcc acgccgcctt cgagcacgcc catgacatct
    15661 ccaacgaggc caagaactcg atccgacgcg ccctgggcct tcccgacgcg gacatcatcg
    15721 tccatgtcgc tccgagccgg gacgaaaccc gcgacatggt cgagcacatc cgggacatcg
    15781 ccgcccggct cgacctggcc gcccatgaaa tccgcttctt cgaagccgac ggccggccgg
    15841 ccctcgacct gcatcttgaa atccgcgggg cggcgaacgt ggccgacgcc cacgcccggg
    15901 cctccgcgtt cgaggaggcc gtccatgccg ccgttcccgg gctcgaccgg attttcaccc
    15961 acttggaacc ggtcggcggc caagccttca tcgcccccct tcctccgggc gaggaccggg
    16021 ccatccgccg gcggatcgct gagctgtccg aggagatggg gatcgtctgc gatccgcacc
    16081 acatcttgat cgaggagacc gtcggggaag tctgcgtctc ctttcactgc agcgtcgatc
    16141 ccgccacgcc cctggccgag gcccataagt tcagcgagcg attcgagatg gccctccggc
    16201 gcgacattcc gcgcgtcggc cgggtcttga tccacatcga gccgccggaa ccggccgg
//
LOCUS       DAOOXH010000055        16089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_3561_length_16089_cov_7.827554, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000055 DAOOXH010000000
VERSION     DAOOXH010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 16089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16089
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3561_length_16089_cov_7.827554"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PKV64_07470"
     CDS             <1..234
                     /locus_tag="PKV64_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HPN16954.1"
                     /translation="QVRGVQKMIEERRYCVDILTQLASVSAALARVEERILERHLRSC
                     VRDSLTRRGRTDQEKKIEEIITLLGRIRRLPGA"
     gene            245..3202
                     /locus_tag="PKV64_07475"
     CDS             245..3202
                     /locus_tag="PKV64_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPN16955.1"
                     /translation="MAIDPICGMTVREDAARFFSDYAGRRWYFCSAFCKKKFDADPET
                     YVWRSRDPLSGRVSTGADSAPDSSPSSSPAPAASFSTPSDSATPASSHSATSPSTPAP
                     TATPAPAPPNAPPGSSPGPFHAAIPAPAPPPLPAPASIPQAAEGTVRLDIPILGMTCA
                     TCAATIEKALKKIPGVVFAAVNFATGRATVIYPPSAVPAADLVAAVRGAGYDVVSASA
                     EIAIKNVMCAGCAATIERALLRRRGVFQAAMNVLTLRARVKYLPTEIGLDELFEAVES
                     AGYQVIRPSPAAAALAQADGRSAGDAEREIRERDLRRLRTKVVIGFALSLVIFLGSMG
                     GVLPFVPAFLRAPVLLWILATPVQFGLGAAFYRGAWAAFRNRRADMNTLVAVGTSAAY
                     VFSAVETAAPSLLSAGGRGQHVYFDTSAVIITLVLFGRFLEARAKGRASEAMKKLLGL
                     QPRTARVLRGGIETDIPPSAVLPGDLVVVRPGERIPVDGLVTKGRSTVDESMITGESI
                     PVEKGEGDEVIGATMNRLGSFTFRAVKVGRETVLARIVRMVEEAQGSKAPIQRLADRI
                     AGWFVPAVIGLAVLTFAAWMIFGPEPALKFALLNFVSVLIIACPCALGLATPTAVMVG
                     AGKGAEYGILIRNGESLETAAKLTTVVFDKTGTLTEGAPALTDIIPGEGWTEGGLLGI
                     AAAAEKGSEHPLAEAVADAVASAAPAVAVPGADSFRSLPGRGLEASVGGRDVLLGNAA
                     LMTERGVDVSAWTDRAENLSAQGKTVMYAAVDGRAAGLFAAADPLKAKSVAAVDRLKT
                     LGLDVVMLTGDHRRTAEAAARAAGIGRFAAGLLPGDKIAEIRRLQKAGERVAMVGDGI
                     NDAPALAAADVGVAIGSGTDVAMEAADITLIGDDLMGVARAVELSRRTIRTIRQNLFW
                     AFIYNIVGIPVAAGVLYPFFGLLLNPMIASAAMAFSSVSVVTNSLRLRRMDLGAPPPK
                     RRAFSQPLASFPE"
     gene            3278..4117
                     /locus_tag="PKV64_07480"
     CDS             3278..4117
                     /locus_tag="PKV64_07480"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HPN16956.1"
                     /translation="MKTKSMGIVLFVLVLGLSAWAAVQTADRKAVDPVCGRMTAAEGA
                     RWTYEYQGTTYAFCCEACKAAFVKEPEKFLAKMKETPAAAAECPMTAGQASAAKEGEP
                     AAFGFPMPGMPARSMSPCCCCCCPRMAHPMHPMPPHAMQPMHPMRPMTPAPAAPAAPP
                     AAEAPAEPSAPAVAPAPPMGSFCPVMRPRMMMGRGMRGMASAPGFGLMQMKDIERKVE
                     NTKDGVVITLTSKDPAAVKMIQEHIALMEKNQANMMYMMMDGSGVKKEIKIDVKEDKK
                     EKK"
     gene            4162..4968
                     /gene="thiM"
                     /locus_tag="PKV64_07485"
     CDS             4162..4968
                     /gene="thiM"
                     /locus_tag="PKV64_07485"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HPN16957.1"
                     /translation="MNADTIWKDIKEIRGDAPLVHNITNFVVMEVTANALLAIGASPV
                     MSHALEDVEDMVGIASSLVLNIGTLSEPWIEAMFRAGEAARRRGLRIVFDPVGSGATR
                     YRTETSLRILEELQPAIIRGNASEIMSLVTSQTVTKGVDSRYGSDKAMDAAFRLTDKY
                     RCVVSVSGAVDRIVFQNEIIKIANGHEMMPRVTGLGCAATALTGAFAAVDPSPFEAAA
                     AAMAVMGIAGEIAAEKAQGPGSFRVRFIDALHAIGENDLRTRLQAATESR"
     gene            4996..5625
                     /gene="thiE"
                     /locus_tag="PKV64_07490"
     CDS             4996..5625
                     /gene="thiE"
                     /locus_tag="PKV64_07490"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HPN16958.1"
                     /translation="MLVTDRGLSAGRPVEEIVEAAVSGGITIVQLREKEAATRDIIAL
                     GRRLAEILRPRRIPLIINDRVDVALAVLADGVHLGAADMSPGDARAILGPKAIIGLSV
                     ENEAQAVAADPRTVDYLGVSPVFATPTKTDTGAAWGIEGLWRLRPLVRLPLVGIGAIK
                     AENAARVMEAGADGLAVVSAICAAPDPAAAARTLRAIVEAARTKRGGAA"
     gene            5668..6735
                     /gene="thiL"
                     /locus_tag="PKV64_07495"
     CDS             5668..6735
                     /gene="thiL"
                     /locus_tag="PKV64_07495"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HPN16959.1"
                     /translation="MKRRKAGRGGSVASREPDGLDRLGEFGFIARMARPFIRELPAGV
                     EGIGDDCAVMPWTKGERLLVTTDMLLENRHFIRNRVSGRDLGWKSLAVNLSDIAAMGG
                     RPKWALLSLGIPEGVGLEWLDAFYKGWREAARPFGVRLVGGDTTRSPGGIVISIVVGG
                     TVRAGKEKLRSGAAAGDVVAVTGAVGDSGGGLKVLLAGGPEGRDEERLVRAHHRPRPH
                     VEEGEWLAGRPGVRAMMDVSDGIDSDIRRIMERSRVGADIALDRLPVSAVLRRVARKR
                     GFEAVETAATGGEDYCLLVTVAPETFERTAGAFKRRFGRLLAAVGTIRTKASGLRYFL
                     NGRPAHIRARGYDHFKSFRNS"
     assembly_gap    6773..6872
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7148..7699
                     /locus_tag="PKV64_07500"
     CDS             7148..7699
                     /locus_tag="PKV64_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HPN16960.1"
                     /translation="MKTVKRKSRAKAEFVLTNEVLLEAAKRKAGVIEARIISPADVVT
                     AAWVKMKCRFGCDGYGQCLVCPPFTPTAEEMRRVLDGYRRAILIHFRPRAAVARAIAG
                     LEREIFLKGAWKAFGLEAGPCPFCRTCPVEEGWCRHPELARPAMEACGIDVFSTVKKA
                     GFPIDVVRTRRQVPDYYGLILVD"
     gene            complement(7820..8224)
                     /gene="ccsA"
                     /locus_tag="PKV64_07505"
     CDS             complement(7820..8224)
                     /gene="ccsA"
                     /locus_tag="PKV64_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPN16961.1"
                     /translation="MWLHVSVTLLGEAFFAVAFITSIMYLRAKDPEKKAKMDSVSYRC
                     VSIGFPLFTLGGLIFGMVWAEKAWGTYWNWDPKEVWSLITWFVFALYLHTRIVMGWKG
                     KRSAFIAILGFLAALFTFFGVNYLLSGLHSYV"
     gene            complement(8203..8607)
                     /locus_tag="PKV64_07510"
     CDS             complement(8203..8607)
                     /locus_tag="PKV64_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16962.1"
                     /translation="MTESAFFTIAFALYAAAALLYLAFLFGKKPKWARAAFIAASVGL
                     ALHTVALVLRTFQSGHAPFTNMYESMSFLAWASVLAFIIIETMFKIPKVGAYVMLFVI
                     AIMALASSPLMPKEAAPSSPPCRATGCGFTSR"
     gene            complement(8702..10243)
                     /locus_tag="PKV64_07515"
     CDS             complement(8702..10243)
                     /locus_tag="PKV64_07515"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HPN16963.1"
                     /translation="MKTIIRFFSSLKLAIVLLILLVAASILGTLIPQGREAADYAARY
                     GRLAEPMVKLRLTDLFRSVWFLGLMALFGLNIAVCTATRLGAKLRRAFRPKVESDPKS
                     LAAGKISARLRIDRSAAEAKETAIRTLRASRYKVRIAEEPETEGRSGTEGAGADARQA
                     GGRIRIHLLGRKGTAGIFGADFVHLGLLVIIAGGLVTAAGGFRTDLGLNAGKTLDVPR
                     AGFALRLDKFATETYADGSVKDWKSTLTVVEDGKDVRTVVIEVNHPLVHRGYSFYQSA
                     WGLDWDNPDLTFWIERKDAPGTGRTSKIRVGEKTAFPEEGTKERRAGQGEGGGKKTAA
                     DQKEKETGMEWTVRRFFPDFVIGDDNRPGTRSGQPNNPAALIEGWRGRRKVIEGWIFA
                     AYPQVNRLRGEEAADWTIELRDADAPQYSVIQAARDPGVPLIWIGCLLVMAGLFLAFY
                     WPAREIRITLEETEGPAAGSAGSGGKGRKRGSGRMEITAGGTASKSRDRFASEFARVT
                     AALKE"
     gene            complement(10626..11081)
                     /locus_tag="PKV64_07520"
     CDS             complement(10626..11081)
                     /locus_tag="PKV64_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPN16964.1"
                     /translation="MIKLSTKGRYGTRLMVNLARQYGNGPKAIILKSVADAEGISIRY
                     LEQIIIPLKINKLVKSIRGAGGGYTLSRSPKEIKVCDILHALEGTCSLVECVEDETSC
                     DKISTCGTHEIWKAASVMMKNYFDSISLADVIEITDKKIAAAKKKTKTK"
     gene            11482..13095
                     /locus_tag="PKV64_07525"
     CDS             11482..13095
                     /locus_tag="PKV64_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPN16965.1"
                     /translation="MSKEILLGDEAVALGAVHAGLTAAYSYPGTPASEIMEFLIRSNG
                     RAGKYVAAWSVNEKTAFEEALGTSFAGGRTLVSFKHVGLNVAADPFMSAALLGINGGF
                     VFVSADDPSMHSSQNEQDSRFYAQFALIPCYEPADHQEAYDMAREAYDHSEKYNVPVM
                     IRLSTRISHSRAGVETRPARDKNPLRRGAREAWTLLPHLARGRNKRLIDLQPELIKSS
                     EASARNKLDLNPANRSFGVIASGIAYNYVMENLAALPADSPSRPSVLKISAYPLPVTL
                     IRRLVDHVETILVAEEGAPFIEQAISGLLGFPGKNITGRLSGALPRSGELSPETVRPA
                     LALTPLPRRPLDKISGRPPQLCVGCPHTDTYKAIKAAASGHPDATVFSDIGCYTLGYF
                     PPHNAIDSCVCMGASVGMAKGASEAGLHPAIAVIGDSTFGHSGMTALRSAAAYNADMT
                     LVILDNCTTAMTGGQPTFASGDRLLKIVEGLGVPKEHIRTIVPLPKNHEANVKVFQEE
                     IAHKGISVIVAVRECLEEAKRKNKAGGAQ"
     gene            13047..13679
                     /locus_tag="PKV64_07530"
     CDS             13047..13679
                     /locus_tag="PKV64_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPN16966.1"
                     /translation="MPRRSETEKQSGRRPVNTIRKQDIILAGVGGQGILSIAFVVDNA
                     ALKEGLNVKQAEVHGMAQRGGAVQSHLRLSSGRIWSDLIPRGGADLILSVEPLEALRY
                     LDFLKPDGRIIASSNPFVNIPDYPDRDEVLAKIKKVPGSLVVDSEKLAKDAGASRAQN
                     TVMLGAASKHLIVSEEHLKDYIRVLFEKRGERILEANLKAFELGKAAADD"
     gene            13672..14094
                     /locus_tag="PKV64_07535"
     CDS             13672..14094
                     /locus_tag="PKV64_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16967.1"
                     /translation="MIDFEDPTPIATPEEFRKALLAVRDSEKGIYDHKELPMLEAQYY
                     SPKHTINTTQLAEKVGFTGYSEANLNYGILAHRIADELKYVPGSPNPNCDHWWRTLSY
                     GKTPDQNEEIQFYKWVMRPELVQALEKMGWVKKNSDLS"
     gene            complement(14102..14881)
                     /locus_tag="PKV64_07540"
     CDS             complement(14102..14881)
                     /locus_tag="PKV64_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16968.1"
                     /translation="MKTSGFDTKEILICVKAYPIASVSYGETVCCAGIDIKSQEWIRL
                     YPVPFRDLESDQRFKKYSIIETKCVKAGDDLRPESYRIQSSSIKILKYLSTDQKWKER
                     KEVVLKAPRTTFCQLKLDQTVHNTSLGLIKPKRVAFEAKKRRGSNLEKKIQAYAQPGL
                     FDKPKKMIEEIHFQFYYHFFCDSAGNCPGHSLSIIDWEIMQAFRKWRTRYLDTAILLE
                     KIKSRWLDIVDPAKKDAFLYVGNLHRFPNEFTVLGVFWPPQ"
     gene            complement(14878..15321)
                     /locus_tag="PKV64_07545"
     CDS             complement(14878..15321)
                     /locus_tag="PKV64_07545"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HPN16969.1"
                     /translation="MNAKYFFTIGYEGRYIEDFVSNLKSHNIQRLIDIREIPISRKKG
                     FSKTILKSILEAENIEYIHIRALGSPSDIRHKLKGNLDFEDFSRLYMNHLLQQAESLQ
                     KVIRDIDEKTSCFMCFEKAPANCHRSLVAKKILDITGQGYQVHHI"
     gene            15519..>16089
                     /locus_tag="PKV64_07550"
     CDS             15519..>16089
                     /locus_tag="PKV64_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HPN16970.1"
                     /translation="MKINNHDYKPFVSAFDPAPQTGRFHGRRVAERIPHRQSKVTTLD
                     AVMDVIRDGDTISYPHYYRTGDTGLKMVVEKLRETGKKGILIYGNAYFDHVDPWLIEA
                     VRDGVIGGLYGNPYRTLGDHITKGEVLPWVTVGVSHGDRVRKLQTGEVRVKVAFGPVP
                     IADRYGNANGLQGKPEELCGPIGLFAADAE"
BASE COUNT         3090 a         4983 c         4714 g         3202 t
ORIGIN      
        1 caggtccggg gcgtccagaa gatgatcgag gaacgccgct actgcgtcga tatcctgacc
       61 cagcttgcct ccgtctcggc ggcccttgcc cgggtcgagg agcgtatcct tgaacgacat
      121 ctccgatctt gcgtccgcga ctccctgacg cggagggggc ggaccgacca agagaagaaa
      181 atcgaggaga tcatcaccct cctcggccgg atccgccgcc tgcccggcgc ttgagatcgc
      241 ccccatggcc atcgacccca tctgcggcat gaccgtccgg gaggacgcgg cccggttttt
      301 ctccgattac gccggccggc gctggtattt ctgttcggcg ttctgtaaga aaaaattcga
      361 cgccgacccc gaaacctatg tttggcgcag ccgggaccct ttgtccggcc gcgtctcgac
      421 cggcgccgat tccgcccccg attcctctcc ctcctcaagc cccgccccgg ccgcttcttt
      481 ctctactcct tcagactccg ccacccccgc ttcttcccac tccgccacct cgccttccac
      541 ccccgctccg actgccacgc ccgcccccgc tccacccaat gccccgcccg gctcctctcc
      601 cggccctttc catgccgcca ttcccgctcc tgccccccct cccctccctg ctcccgcctc
      661 catcccccaa gccgccgagg gaaccgtccg tctcgacatc cccattttag gaatgacctg
      721 cgcgacttgc gccgcgacga ttgaaaaagc cctcaaaaaa attcccggcg ttgtcttcgc
      781 ggccgtcaat ttcgccaccg gccgggcgac cgtgatttat ccgccgtccg ccgtcccggc
      841 ggccgatctc gtcgcggccg tccgcggcgc cggctacgat gtcgtctcgg cctcggccga
      901 gatcgcgatc aagaacgtca tgtgcgccgg ctgcgccgcg acgatcgagc gcgcccttct
      961 ccgccgccgc ggcgtgttcc aggccgcgat gaacgtcctt accctccggg cccgggtgaa
     1021 gtatctcccg acggaaatcg gcctcgacga gctttttgaa gccgtcgagt cggccggcta
     1081 ccaggtcatc cgcccttccc ccgccgccgc cgccctcgcc caagccgacg gccgctcggc
     1141 cggcgacgcg gagcgcgaaa tccgcgagcg cgatcttcgc cggcttcgga cgaaagtcgt
     1201 gatcggcttc gccctcagcc tcgtcatttt tctcgggagc atgggcggcg tccttccctt
     1261 cgttcccgct tttctccgcg cgcccgtcct tctctggatc ctggcgactc cggtccagtt
     1321 cgggctcggg gcggcgttct atcgaggcgc gtgggcggct ttccgcaacc gccgggcgga
     1381 catgaacacc ctggtggccg tcgggacgtc ggcggcctac gtgttcagcg ccgtcgagac
     1441 cgccgccccc tcgcttctgt ccgccggcgg ccgcggccag cacgtttatt tcgatacgtc
     1501 ggccgtcatc atcaccctcg ttctgttcgg ccgctttctg gaagcccggg ccaaggggcg
     1561 ggcctccgag gcgatgaaga aactcctcgg ccttcagcct cgaaccgccc gcgtcctccg
     1621 cggcggcatc gagaccgata tcccgccgtc cgccgtcctc cccggcgacc tggtcgtcgt
     1681 ccggccgggg gagcggatcc cggtggacgg gctggtgacg aagggacgct cgacggtgga
     1741 cgaatcgatg atcaccggag aaagcatccc ggttgaaaaa ggcgagggcg acgaggtcat
     1801 cggggcgacc atgaaccggc ttgggagctt caccttccgg gcggtcaagg tcggacggga
     1861 gacggtcctg gcccggatcg tccggatggt cgaggaggcc cagggctcga aagcccccat
     1921 ccagcgcctg gccgacagga tcgccgggtg gttcgttccg gcggtcatcg gcctggccgt
     1981 cctgacgttc gccgcctgga tgatcttcgg tccggaaccg gccttgaaat tcgccctgct
     2041 caatttcgtt tccgtcctga tcatcgcctg tccctgcgcc ctcggcctgg ccacgcccac
     2101 ggcggtcatg gtcggggcgg gcaaaggggc cgagtacggc atcctgatcc gaaacggcga
     2161 gagcctcgag acggcggcca agctgacgac cgtcgttttc gacaagaccg gaaccttgac
     2221 cgagggcgcc cccgccctga ccgacatcat tcccggcgaa ggatggaccg aaggcggact
     2281 cctggggatc gccgccgccg ccgagaaagg ctcggagcat ccgctggccg aggccgtcgc
     2341 ggacgcggtt gcgtccgccg cgccggcggt cgccgtgccc ggggccgact ccttccgctc
     2401 cctgccgggg cgcgggctcg aagcctcggt cggcggccgg gatgttcttt tgggaaacgc
     2461 cgccttgatg accgagcgcg gggtggacgt gtcggcttgg acggatcggg ctgaaaattt
     2521 gtcggcccag ggaaaaacgg tgatgtacgc ggccgtcgac ggccgggcgg ccggcctttt
     2581 cgccgcggcc gatccgctca aggccaaatc cgtggccgcc gtggaccggc ttaagacgct
     2641 gggcctggac gtcgtgatgc tgaccggcga ccatcgccgc acggccgagg cggcggcccg
     2701 ggcggcggga atcgggcgtt tcgcggccgg actcctgccc ggggataaaa tcgcggaaat
     2761 ccggcggctc cagaaggccg gggagcgcgt ggccatggtc ggcgacggga tcaatgatgc
     2821 gcccgccctg gccgcggccg acgtcggcgt ggccatcggc tcggggacgg atgtggccat
     2881 ggaagccgcc gacatcaccc tgatcggcga cgatcttatg ggcgtggccc gggccgtcga
     2941 gctcagccgc cggacgatcc ggacgatccg gcagaacctg ttttgggcgt tcatctacaa
     3001 catcgtcggg atcccggtcg cggccggcgt cctctacccg ttcttcggcc tcctcctgaa
     3061 cccgatgatc gcctcggcgg cgatggcctt cagctcggtg tcggtcgtga cgaactcgct
     3121 tcggttgcgc cggatggatt tgggggcgcc gcctccgaaa aggagggcgt tttctcaacc
     3181 cctcgcttca tttccggaat gaaggatgcc ccccaaattg atatttccga ttggagaagg
     3241 tagaatgaat cgcttgaaaa catatggagg cagaaaaatg aaaaccaagt ccatgggaat
     3301 cgtgctgttt gttcttgttt taggattgtc ggcttgggcc gcggtccaga ccgccgaccg
     3361 caaggccgtc gatcccgtct gcggccggat gacggccgcg gaaggcgccc ggtggaccta
     3421 cgagtatcaa ggaacgacgt acgcgttctg ctgcgaggcc tgcaaggccg ccttcgtcaa
     3481 ggagccggaa aaattcctgg ctaaaatgaa agaaacgccg gcggccgcgg ccgaatgccc
     3541 gatgacggcc ggacaggcct cggccgcgaa ggaaggcgaa cccgccgcct tcggcttccc
     3601 catgcccgga atgccggccc ggtcgatgtc cccctgttgc tgctgctgtt gtccgagaat
     3661 ggcccatccg atgcatccca tgccgcccca cgcgatgcag ccgatgcatc ccatgcggcc
     3721 gatgaccccg gctcccgcgg ctccggccgc tcctccggcc gcggaagcgc cggccgagcc
     3781 ttccgctccg gccgtcgccc cggcgcctcc gatgggctcc ttttgcccgg tcatgaggcc
     3841 gcggatgatg atgggaagag gaatgagagg catggcctcc gccccgggct tcggcttgat
     3901 gcaaatgaaa gacatcgaaa gaaaggtcga gaacaccaag gacggcgttg tcatcaccct
     3961 gacgtccaag gatccggccg cggtcaaaat gatccaagag catatcgccc tgatggaaaa
     4021 aaaccaggcg aacatgatgt acatgatgat ggacggaagc ggggtgaaaa aagaaatcaa
     4081 gatcgacgtc aaggaagaca agaaagagaa gaaataagga ccggcctgga gaatcgtttt
     4141 catcgaggag gaagacgtct aatgaatgcc gacacgatct ggaaagacat aaaggagatc
     4201 cgcggggatg cccccctcgt tcacaacatc accaactttg tcgtcatgga ggtgacggcc
     4261 aacgccctcc tggccatcgg cgcctccccg gtgatgagcc acgccctcga ggacgtcgag
     4321 gacatggtcg gcatcgcttc ctctcttgtc ctcaacatcg gaacgctcag cgaaccgtgg
     4381 atcgaggcga tgttccgtgc gggtgaggcg gcccggcgtc gcggcttgcg catcgttttc
     4441 gatcccgtcg ggtcgggggc gacccggtac cggacggaaa cctcgctgcg gatcctggag
     4501 gagctccagc cggcgatcat ccgcggcaac gcctcggaaa tcatgtccct tgtcacctcc
     4561 caaaccgtga ccaagggcgt ggacagccga tacggttcgg acaaagcgat ggacgcggct
     4621 ttccggctga ccgataaata ccgctgtgtc gtcagcgtca gcggggccgt cgaccgcatc
     4681 gtcttccaaa atgagatcat caagatcgcc aacggccacg agatgatgcc gcgggtcacg
     4741 gggctcggct gcgcggccac cgccctgacc ggggcgttcg ccgccgtcga tccctcgccg
     4801 ttcgaggcgg cggccgcggc catggccgtc atggggattg cgggcgaaat cgcggcagaa
     4861 aaagcccaag gcccgggatc tttccgcgtt cgtttcatcg atgccctcca tgcgatcgga
     4921 gagaacgacc tccggacccg cctccaggcg gccacggaat cgcggtgacc ggtggggaaa
     4981 gctgatttcg ggctgatgct ggtcaccgac cgcggtcttt ccgcgggccg gccggtcgag
     5041 gaaatcgtcg aagcggcggt ttcgggggga attacgatcg ttcagctccg ggagaaggaa
     5101 gcggcgacgc gcgatatcat cgccctcggg cggcggctgg ccgaaatatt gcggccgcgc
     5161 cgcattccct tgatcatcaa cgaccgggtc gacgtcgctc tggccgtttt agccgacggc
     5221 gtgcacttgg gtgcggccga tatgagtccc ggcgacgcgc gcgcgatcct gggacccaag
     5281 gcgattatcg ggctgtcggt ggaaaacgag gcccaggcgg tcgcggccga tccgcggacc
     5341 gtcgattatt taggcgtcag ccccgtgttc gcgacaccga ccaagaccga tacgggcgcg
     5401 gcttggggga tcgaggggct gtggcggctg cggccgctcg tccggctgcc gttggtcggc
     5461 atcggcgcga tcaaggccga gaacgcggcc cgggtcatgg aggcgggagc cgacggcttg
     5521 gccgtcgtct cggcgatctg cgccgcgccc gatccggccg cggcggcccg gacgctgcgg
     5581 gcgatcgtcg aggcggcgag gacgaagcgg ggaggagcgg cctagcgggc ggaagcggaa
     5641 gacgaagggg aaaacgaagg gcgcgaggtg aagagacgaa aggcgggcag ggggggaagc
     5701 gtggcgtccc gggagccgga tgggctggac cggctgggcg agtttggatt catcgcccgg
     5761 atggcccggc cgttcatccg ggagctgccg gccggcgttg agggaatcgg cgacgactgc
     5821 gccgttatgc cgtggacgaa aggcgaacgg ctgctcgtga cgacggacat gcttctcgaa
     5881 aaccggcatt tcatccggaa ccgggtgagc ggccgggatc tgggctggaa atccctggcc
     5941 gtcaacctga gcgatatcgc ggccatgggc ggccggccga aatgggccct gctgtcgctg
     6001 gggattccgg aaggagtcgg gctggagtgg ctggacgcgt tttataaagg atggcgtgaa
     6061 gcggcccggc cgttcggcgt ccggctggtc ggcggggaca cgacccggtc gccggggggg
     6121 atcgtcatca gcatcgtcgt cggcgggacg gtccgggcgg gaaaagaaaa actccggtcg
     6181 ggagccgcgg ccggcgatgt cgttgctgtg accggggcgg tgggcgattc gggcggcggg
     6241 ctgaaggtct tgctggccgg cgggccggag gggcgggacg aggagcggct tgtccgggct
     6301 catcatcggc cgaggccgca cgtggaggaa ggggagtggc tggcgggacg gccgggcgtg
     6361 cgggcgatga tggatgtgtc cgacgggatc gactccgata tccgccggat catggagcgt
     6421 tcgagagtcg gggcggacat cgcgctggac cggctgccgg tttcggcggt tctccggcgg
     6481 gtcgcccgga agcgcggatt tgaggcggtc gagacggccg cgacgggagg ggaagattat
     6541 tgcctgcttg tcacggtcgc cccggaaacc tttgagagga cggccggggc gtttaagcgg
     6601 cggttcggcc ggctgcttgc ggccgttggc acgatccgta ccaaggcatc gggtttacgg
     6661 tattttttga acggccgtcc ggcgcatatc cgcgcccgcg gctacgacca cttcaaatca
     6721 tttcgcaatt cttaatccgt acaatctaat cggtacctgt ttaaccattt ccnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnacctgttt aaccatttcc tcaattccgg
     6901 tactcatcaa tccgtacctg tttatctatt tccgcatatc caatccgcac ccggcccaat
     6961 cagtcaatct catctgaacc tgtttaacca tttccgcatt cttttgaaga tacgatctct
     7021 cattcccaat tcgtcgattc ccgacgtttt gggaaacgaa gtttatgggt attgtgtctc
     7081 cctattttct gcgctaactt aatatcagac gatgcggcgc gcggacgcgc ggggagaaat
     7141 cacggctttg aaaaccgtta aaagaaagag tcgagcgaag gcggaattcg tcctcacgaa
     7201 cgaggtgctt cttgaggcgg ctaagcggaa agccggcgtg atcgaagccc ggatcatctc
     7261 tccggccgac gtggtcacgg ccgcttgggt gaaaatgaag tgccgcttcg gctgcgacgg
     7321 atacggccaa tgtctggttt gtccgccgtt tacgccgacg gccgaggaga tgcgccgcgt
     7381 tctggacgga taccggcggg cgatcctgat tcatttccgg ccgcgggcgg ccgtcgccag
     7441 ggcgatcgcc ggactggaac gggaaatctt tctcaaaggg gcttggaagg cgttcgggct
     7501 ggaggcgggt ccgtgtccgt tctgccggac atgtcccgtg gaggagggct ggtgccgaca
     7561 cccggagctg gcccggccgg cgatggaagc ctgcggaatc gacgtgtttt ccacggtcaa
     7621 gaaagcagga tttccgattg atgtcgtccg cacgcggcgc caggtcccgg attattacgg
     7681 attgatcctg gtggattgat tccgccttct ctggaagacg aagcatccct tttccagctc
     7741 atcgattccc gatgtttttg gaaatgagat taacagatat tgtgtctccc tgttttattg
     7801 agtgcggcgt tctttacgat cagacgtaag aatggaggcc ggagaggaga taattcacgc
     7861 cgaaaaacgt gaacaacgcc gccaggaagc cgagaatcgc gataaacgcc gaccgctttc
     7921 ccttccagcc catcacgatc cgggtgtgaa ggtatagagc gaagacaaac cacgtgatca
     7981 ggctccaaac ttcctttgga tcccagttcc agtacgtccc ccaagccttt tccgcccaaa
     8041 ccatcccgaa gatcagtccg cccagcgtga acaacggaaa gccgatcgag acgcaccggt
     8101 agctcaccga atccatcttc gcttttttct ccggatcctt ggcccgcagg tacatgatgc
     8161 tggtgatgaa ggccacggcg aaaaaagctt ccccgaggag cgtcaccgag acgtgaagcc
     8221 acagccagta gctctgcagg gcggggacga gggagccgcc tccttgggca tcagcggcga
     8281 ggaggccagg gccatgatgg cgatcacaaa cagcatcacg taagccccga ctttgggtat
     8341 tttgaacatc gtctcaatga tgatgaacgc caggaccgaa gcccaggcga gaaacgacat
     8401 cgactcgtac atgttcgtaa acggcgcatg cccgctttgg aaggtccgca agacgagggc
     8461 gacggtgtgg agggccagcc cgacggaagc ggcgatgaac gcggcccgtg cccatttcgg
     8521 ctttttgccg aacagaaagg caaggtaaag aagcgcggcc gcggcataaa gggcaaacgc
     8581 gatcgtgaag aacgccgact ccgtcatttc gacctcctcc tcttaatcgg aacctggtta
     8641 accatttcca tattttacct ggagaacatg gaaatacaga aatggttgaa caggtaccga
     8701 attactcttt caaggcggcc gttacccggg cgaattccga cgcgaaacgg tcccggcttt
     8761 tcgaagccgt cccaccggct gtaatctcca tcctgcccga gccccgcttt cttcccttcc
     8821 ctccggaacc cgccgagccc gccgccggtc cttccgtctc ctccagggtt atccggatct
     8881 cccgcgccgg ccagtaaaac gccagaaaca gcccggccat caccaagagg catccgatcc
     8941 aaatcagcgg cacgcccgga tctctggccg cctggatcac cgaatactgg ggcgcgtcgg
     9001 cgtcgcggag ctcgatcgtc cagtcggcgg cctcctctcc ccggagccgg ttgacctggg
     9061 gatatgccgc gaaaatccag ccctcaatga ctttgcgccg gccgcgccat ccttcgatca
     9121 gcgccgccgg attgttcggc tggccggagc gcgtgcccgg ccggttgtcg tcgccgatca
     9181 cgaagtcggg gaaaaaccgc cggaccgtcc actccattcc ggtctctttt tctttctgat
     9241 ccgcggccgt cttttttcct ccgccttccc cctggccggc tcttctttcc tttgttcctt
     9301 cttccgggaa ggctgttttt tccccgaccc ggattttcga cgtccgcccc gttcccggcg
     9361 catccttccg ttcgatccaa aacgtcaggt ccggattatc ccagtcgagg ccccaggcgc
     9421 tttggtaaaa cgaatatccg cgatgaacga gaggatggtt gacctcgatg accacggttc
     9481 gcacatcctt tccgtcctcg accacggtca aggtgctttt ccagtccttg acgcttccgt
     9541 ccgcgtatgt ctcagtcgcg aatttatcta gccgcagggc gaatccggcc cgcggcacgt
     9601 ccaaagtttt cccggcgttg agaccgaggt cggttcggaa tccgccggcc gcggtcacga
     9661 ggcctccggc gatgatgacg agcaaaccca ggtggacgaa atccgcgccg aaaataccgg
     9721 cggttccctt gcggccgaga agatgaatcc ggattcttcc gcctgcttgt ctggcgtccg
     9781 cgccggcgcc ttccgttccc gatcggccct ccgtctccgg ctcctcggcg atgcgcacct
     9841 tgtaccgcga ggcccgcagc gtccggatcg cggtttcctt cgcctcggcc gcgctcctat
     9901 cgatccgcag ccgtgcgctt atctttccgg cggccaacga tttcggatcg ctctcgactt
     9961 tcggccgaaa cgcccgtctc agcttcgcgc caaggcgcgt cgccgtacaa acggcgatgt
    10021 tcaagccgaa caaagccatc aatcccaaga accaaacaga gcggaaaagg tcggtcaatc
    10081 gaagcttgac catcggttcg gcgagccggc cgtagcgggc ggcatagtcg gccgcctccc
    10141 gcccttgggg aatgagcgtg cccaggatcg aggccgcgac cagcagaatg aggaggacga
    10201 tggccagctt gagggaggaa aagaatcgga tgattgtttt catgtctttg tcagcggaat
    10261 cttaccggaa atcagccgcc catttctaga ggcggccgcc gattggggtt tttccggacc
    10321 gggaggggac ggcctcctcc tgttcccgaa atccgagttc gggaatccga attcggtacc
    10381 tgtttatcca tttctcgaat cggccggcga tggcttgtca ttttataaat cccgcgttat
    10441 ctgcaagctc cgggatcgat atagcgaaaa ggataagcag gttgggatat aaaggaaatt
    10501 tgatatgggg gaatggttga acaggttcgg aattaatgag caggcgccgg atgagatgca
    10561 gcggcggggt taatgaggca agaggcgtta tttctcccgg ttgcgatggg ggagagacca
    10621 cccggctact tggttttcgt cttcttcttg gccgcggcga ttttcttgtc cgtgatctcg
    10681 atgacatcgg ccagggagat gctgtcgaaa taattcttca tcatcaccga ggcggctttc
    10741 cagatttcgt gcgtcccgca agtggatatc ttgtcgcagc tcgtctcatc ctcgacgcat
    10801 tcgaccaggc tgcatgtgcc ttccagggcg tggaggatat cgcagacttt gatctcttta
    10861 gggctgcggg agagagtgta tccgcctccc gccccgcgaa tgcttttaac gagcttgttg
    10921 attttcaacg ggatgatgat ctgttcaaga tagcggatgg aaattccttc cgcgtccgcc
    10981 acgctcttga ggatgatagc tttggggccg ttgccgtatt gacgggccaa gttgaccatc
    11041 agacgcgtcc cgtatcttcc cttcgtcgaa agcttgatca tgccgcgttt ctccttcttg
    11101 tcgtcagccg atcacgctcg cgctttcggc cgcaatgcgg ccttatcata gcccattcgc
    11161 atgccggttt caacataata ttataggatt tatatattcc ccagatttat gggaaaatcg
    11221 cgaatatttc caggattggt acctgtttat ccatttccca aatcggccgg agacggcatg
    11281 tcatttctta aattccgcat catctgcaag ttccgggaac gaaacaggga aatggataag
    11341 caggttcggg tataaaggaa atttgatatg gggaaatggt gaaacaggtt ctgatttatg
    11401 atttaccgat ttaattgaca accccatccc caggcgatat tctaccctct cccaacttca
    11461 tcccatggaa aggacgtgtc catgtccaag gaaatccttc tcggcgacga agccgtcgcc
    11521 ctcggcgcgg tccatgccgg cctgaccgcc gcctacagct accccggcac gccggcctcg
    11581 gaaatcatgg agtttctcat ccggtccaac ggccgggccg gtaaatacgt cgccgcctgg
    11641 tcggtcaacg agaaaaccgc cttcgaggag gccctcggca cctcgttcgc gggcggacgg
    11701 acgctcgtct ccttcaagca tgtcggcctc aacgtcgccg ccgatccgtt catgagcgcc
    11761 gccctgctcg ggatcaacgg cggattcgtt ttcgtctcgg ccgacgaccc aagcatgcac
    11821 agctcccaaa acgagcaaga cagccgcttc tacgcccagt tcgccctcat tccctgttat
    11881 gagccggccg accaccaaga ggcttatgac atggcccgcg aagcctacga ccattcggaa
    11941 aaatacaacg tcccggtcat gatccgcctc tcgacccgga tctcccactc ccgggccggc
    12001 gtcgaaaccc gccccgcccg cgataaaaat cccctccgcc gcggcgcccg tgaggcctgg
    12061 acgctccttc cgcacctggc ccgcggccgc aacaagcgcc tcatcgacct ccagcccgag
    12121 ctcattaaat cctccgaagc cagcgcccgc aacaagctcg acctcaatcc ggccaaccgc
    12181 tccttcggcg tcatcgcctc cggcatcgcc tataactacg tgatggagaa cctcgccgcc
    12241 ctccccgccg attccccctc ccgtccatcc gtcctcaaga tctcggccta cccgcttccc
    12301 gtcacgctca tccgccgcct cgtcgaccac gttgaaacga tcctcgtcgc cgaggaaggc
    12361 gccccgttca tcgagcaggc catcagcggc cttctcggct ttccgggcaa gaatatcacc
    12421 ggccgcctct ccggcgcgct gccccgatcc ggggagcttt cgcccgagac cgtccgcccg
    12481 gccctcgccc ttaccccgct cccgcggcgt cccctcgaca agatctccgg ccgccctccg
    12541 cagctctgcg tcggctgtcc tcacacggac acgtataaag cgatcaaggc ggccgcctcc
    12601 ggccaccccg acgcgaccgt cttttccgac atcggctgct acaccctcgg ttatttccct
    12661 ccccacaacg ccatcgactc ctgcgtctgc atgggcgctt cggtcgggat ggccaaggga
    12721 gcgtccgaag cgggcctcca tccggcgatc gccgtcatcg gcgactcgac cttcggccat
    12781 tccgggatga cggccctccg ctcggcggcc gcctacaacg ccgacatgac cctggtcatc
    12841 cttgacaact gcaccacggc catgaccggc ggccagccga ccttcgcctc cggcgaccgg
    12901 ctcctcaaga tcgtcgaggg cctgggcgtg cccaaggaac acatccggac catcgtcccg
    12961 ctgcccaaga accacgaagc caacgtcaag gtcttccagg aggagatcgc ccacaagggc
    13021 atctcggtca tcgtggccgt ccgggaatgc ctcgaagaag cgaaacggaa aaacaaagcg
    13081 ggaggcgccc agtgaacacc atccgcaagc aagacatcat cctggccggt gtcggcgggc
    13141 aggggatcct ctcgatcgcc ttcgtcgtcg acaacgccgc cctcaaggaa ggcctcaacg
    13201 tcaagcaggc cgaagtccac ggcatggccc agcgcggtgg cgccgtgcaa tcgcaccttc
    13261 gcctctcctc cggacggatt tggagcgacc tcatcccgcg cggcggggcc gacttgatcc
    13321 tgagcgtcga gccgctggaa gccctccgct atctcgactt cctcaagccc gacggccgga
    13381 tcatcgccag ctccaacccc ttcgtcaaca tccccgacta tcccgacagg gacgaggtcc
    13441 tggccaagat caagaaggtt cccggctcgc tcgtcgtcga ctcggaaaag ctggccaagg
    13501 acgccggcgc gagccgggcc cagaacacgg tgatgctcgg ggccgcttcc aagcacctga
    13561 tcgtcagcga ggaacacctc aaggattaca tccgggtgct cttcgaaaag cgcggcgaac
    13621 gcatcctgga agccaatctc aaggcctttg agctggggaa ggcggcggcg gatgattgat
    13681 tttgaggatc caactcctat tgccacgccg gaggagtttc ggaaagcgtt actcgccgtg
    13741 cgtgattcgg aaaaagggat ttacgatcat aaagaattgc cgatgctcga ggcgcagtac
    13801 tattctccaa agcatacaat caatacgact caattagcag aaaaagtagg ttttaccggt
    13861 tattctgaag ctaatcttaa ttacgggatt cttgcgcatc gtatagctga tgaattgaag
    13921 tatgtcccgg gaagtcctaa cccgaattgc gatcattggt ggcggacatt atcatatggg
    13981 aaaacgcctg accaaaacga agaaatacaa ttttataagt gggttatgcg tccggaactc
    14041 gttcaagcgc tggaaaagat gggatgggtg aaaaaaaatt ccgatttgtc ttgagttggg
    14101 attattgagg cggccagaaa acgcctaaaa ccgtaaactc gttcgggaaa cgatgaaggt
    14161 ttccaacata caaaaaagcg tctttttttg ccggatcgac aatatcgagc catctgcttt
    14221 ttattttttc caataatatc gcggtatcca aatatctcgt tcgccatttt cgaaacgctt
    14281 gcataatttc ccagtcgata attgacaacg aatggccggg gcaatttccc gctgaatcgc
    14341 agaaaaaatg atagtagaat tggaaatgaa tttcttcaat catttttttg ggtttatcga
    14401 ataagccggg ctgtgcgtat gcttgaattt tcttttccaa attcgaacct cgcctctttt
    14461 tcgcctcgaa agcaactcgc ttcggtttga tcagtccgag cgatgtgtta tgaacagttt
    14521 gatcgagttt taattgacaa aaggttgtcc gaggagcttt caatacgacc tcttttcttt
    14581 ctttccattt ttgatcggta gataaatatt tcaggatttt aattgatgaa gactgaattc
    14641 gataactttc gggacgaagg tcatcgccgg ctttgacgca ttttgtctcg atgatactgt
    14701 attttttaaa cctttggtcc gattcgagat ctctaaatgg aacaggatat aagcggatcc
    14761 attcctgtga tttgatatcg attccggcgc aacagacggt ttcgccgtag gacacgctgg
    14821 cgataggata ggctttaacg catatgagaa tttcttttgt atcaaaaccg gatgttttca
    14881 aatatgatga acctgatatc cttgaccggt gatatcaaga attttcttcg cgacgagcga
    14941 tcgatggcag tttgccggag ctttttcaaa acacatgaaa caggaggttt tttcgtcgat
    15001 gtcgcggatg actttttgaa gcgattcagc ctgctgaagg agatgattca tgtataaacg
    15061 ggaaaaatcc tcaaaatcga gattgccttt caatttatgt ctaatatccg atgggcttcc
    15121 caacgcacga atatggatgt attcgatatt ttccgcttcc agtatgcttt tcaaaatcgt
    15181 ttttgagaaa cccttctttc gtgagatggg gatctcccgg atatcgataa gccgttgaat
    15241 attatgggat tttagattcg aaacgaaatc ttcgatatac cggccttcgt agccgatcgt
    15301 aaaaaagtat ttcgcgttca tcttcttcgg atattatata ccggtgccgt cataatattt
    15361 agacgttagg aaatttaaaa attctcaaag cgggtgtatt tctcttcagg tcccttgata
    15421 aaacagcccg aatcggatac cttttctcct agcttcattg accccccctg tctgatatga
    15481 ccttggcggg ccggaaaatc atcaaaggag catctcatat gaaaatcaac aatcacgact
    15541 ataagccctt cgtctcggcc tttgatcccg ccccccaaac cggccggttc cacggccgcc
    15601 gcgtggccga gcgcatcccc caccgccagt ctaaagtcac cactctcgac gccgtcatgg
    15661 acgtcatccg cgacggtgac accatctcct atccgcatta ttaccggacc ggcgacaccg
    15721 gcctgaagat ggtcgtcgaa aagctccggg agaccgggaa aaaaggcatc ctcatctacg
    15781 gcaatgccta cttcgaccac gttgatccct ggctgatcga agcggtccgc gacggggtga
    15841 tcggcgggct ctacggcaat ccctaccgga cgctcggcga tcacatcacg aaaggggagg
    15901 tcctcccctg ggtgaccgtc ggggtttccc acggcgaccg agtccggaag ctccagaccg
    15961 gcgaggtgcg ggtcaaggtc gccttcggcc ccgtccccat cgccgaccgc tacggcaacg
    16021 ccaacggcct ccagggcaag ccggaagagc tctgcggccc gatcgggctg ttcgcggccg
    16081 acgccgagt
//
LOCUS       DAOOXH010000056        16051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_3569_length_16051_cov_7.626719, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000056 DAOOXH010000000
VERSION     DAOOXH010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 16051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16051
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3569_length_16051_cov_7.626719"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PKV64_07555"
     CDS             <1..967
                     /locus_tag="PKV64_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPN16971.1"
                     /translation="GTFIAASYETVNFLGAGETLNLTAQYGKRIKNYSFGFTEPYLFD
                     RPISAGFNIYDRYLNYYELYNRKGRGVDLTVNARLLGYLRGSLTYGFEYVDITVSESY
                     GSYKEYLEMYGMGHYYMSSLYPSIYRSTIDSPLTPSSGTLYSASLKYAGTFLGGEVHL
                     YKPRLEFTHYQPIIRGSSWHSIGFHVEGSYIKPLRDTTTPFWERFFLGGERSIRGYEV
                     YTIGPRSPSGTLLGGEKMLVMNFEYIWALGGPISLILFHDRGNAWAGNQKMSLTDVYT
                     STGIEARIFVPALRVPFRLIFSYNNRRIYDSDSKFAFRFAVGTTF"
     gene            1003..1533
                     /locus_tag="PKV64_07560"
     CDS             1003..1533
                     /locus_tag="PKV64_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPN16972.1"
                     /translation="MRKSVIAAALAVLCLGAAANLSFAQGKIGVLDSQQVLERSAEGK
                     KVIARLQESEKKNTAAVTALDDQIRQIQTKLNTGRLTMTDEAALQLQADLERKTTERK
                     RVAEDAVAGMQELSNRLFMKLQSELMPIVQAVGKEKGLDIIFDVQKSGAVYWAPAVDV
                     TEDIIKRYDASKASEK"
     gene            1553..2005
                     /gene="fabZ"
                     /locus_tag="PKV64_07565"
     CDS             1553..2005
                     /gene="fabZ"
                     /locus_tag="PKV64_07565"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HPN16973.1"
                     /translation="MLNIEKIIHILPHRYPFLLVDRVLELEKSKRIVALKNVTYNEEF
                     FQGHFPEIRIMPGVLIVEAMAQAGGILLFHSIPDPETKFVLFSKIDDMKFRRPVIPGD
                     QIRIEAHVLRNRNRFYTLHAKAFVEGEIAAEGDMVASMLEREEFYGRK"
     gene            1992..2810
                     /gene="lpxA"
                     /locus_tag="PKV64_07570"
     CDS             1992..2810
                     /gene="lpxA"
                     /locus_tag="PKV64_07570"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPN16974.1"
                     /translation="MAGSDLRIHPTAVVHPGARIDPGAVIGPFCLIGPEAVVGPGTVL
                     ESHVTVGGRTTIGADNRVSPFVAIGGEPQDVGYKDEPSRIQIGDRNVIREFVTIHRAT
                     TKQDLETVIGDDNYLMAYSHVAHDCRLGRGIIMLHGATLGGHCQVGDFALVGAMSGVH
                     QFCRLGRFSFMGGGSMVTQDVLPFCRVAGQRPTRALGLNVVGLRRNGFSRERISALKA
                     IFQILLFENLNTTQALERIEAAFPPSEDREEIAAFIRTAKRGYVKKSADAWDRD"
     gene            2795..3601
                     /gene="lpxI"
                     /locus_tag="PKV64_07575"
     CDS             2795..3601
                     /gene="lpxI"
                     /locus_tag="PKV64_07575"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HPN16975.1"
                     /translation="MGSRLGLIAGAGELSRAVLAEAKKSGLFCAVAGLRGAAPAGLER
                     EADAFAWFGPADLDELAAFFRGHGIREAVMFGKIEHRLIFGEALGNGAIRALAAALPD
                     HSPSTLLRALIEYLGAAGIAVCDPSRFLEPYGCPEGVLVPAAAPEAVETETAFGWPLV
                     RTLADLDIGQTLVVKDRAVVAVEGMEGTDETVRRAAALAGPGVVVLKAARTRQDPRVD
                     LPMVGLETVRTLVEARAAALVVEAGRVPFFQREEARAAAAAGGVALIGRR"
     gene            3613..4530
                     /locus_tag="PKV64_07580"
     CDS             3613..4530
                     /locus_tag="PKV64_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN16976.1"
                     /translation="MEKIKLGIIGVGYLGMQHARIASYLEEADLRAVADIEFKKAFEI
                     GNRHGVRYYQEFEEMLDDIDAGIVAVNTSSHYGVAKRLLERGKHVLVEKPITETVGQG
                     EELIALARKNGLILQVGHLERFNPAVEAVENIISEPKFIEVQRLGSFSARSLDVDVVL
                     DLMVHDLDIIMALIKDEVSVIRSTGIHVVSDKVDIANARLEFKGGCVATLTASRVHQG
                     KVRKLRIFEPTAYYAIDYIDQEVKIFPLSGRQTDIKSFKIKKEEPLKKEILNFLACIA
                     EGRTGKVSGEEGLQALRLAHDVLKEMETT"
     gene            complement(4567..5292)
                     /locus_tag="PKV64_07585"
     CDS             complement(4567..5292)
                     /locus_tag="PKV64_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16977.1"
                     /translation="MKKRLLIGVCAALGILCLGLQAQELTVADILSKSLEAQGGRTAL
                     AAIKDTTTSGTIEITQMGMSAALTIYQKEPNMMRMDIDVMGMLITQAFDGEKGWFINP
                     QTGVAEEMNEKQSQAQRLQAMGYDAALNPEKYGITFVLKGKEKVGDKDYYLVEQTFKD
                     GFQATLYINTATFLTEQIRSKTDAMGTEIDVETLMDDFKNVEGLMVPHSLTILQGGAE
                     FGRVTVAKVVFNTGLEDSFFKMQ"
     gene            5715..7052
                     /locus_tag="PKV64_07590"
     CDS             5715..7052
                     /locus_tag="PKV64_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16978.1"
                     /translation="MKMRNIRNLKRRAAWLVWPAAVLILAAGLLAAEDSDAGGPQTAA
                     QIAEWKKSAPRVYLDFEKGDPDYIRTEIPFVNYVRDRKEADIHILVTTQTTGGGGIEY
                     AMAFLGQGDFASLSGDLIYASAKTDTEEEVRKGYVAVLKMGLVPFVARTPIRDLLSIE
                     YREKIQPTDVVDPWRFWVFSLSVGGELDHESQAKSQAMWLNASANKITPDMKIRLGLS
                     TYFARDEFRFDEELIESDSRGLDLAGLFTKSLGEHWSAGVYVSAGSSTYANIRTKISP
                     APVLEYNIFPYSESTRRQLRIMYKVGFDFVRYREETIYDKTRENLTSQALSAVLEIKE
                     PWGTLEAGLEGSHYFHDFKKNRLEFNGEAAIRILRGLDFVVDLHYERVRDQLSLAKGN
                     RSLEEILLRRKELATDYNLGLTVGLSYTFGSIFSNVVNPRFGDLGNLDYHSHD"
     gene            complement(7071..8441)
                     /locus_tag="PKV64_07595"
     CDS             complement(7071..8441)
                     /locus_tag="PKV64_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPN16979.1"
                     /translation="MDTAISFLVVGRDASIYERLRDSGLDPDISLFFCGRDENVLDFI
                     QTHAIRSLLLDASADPEGTRALLRELKSFDPLLQIVVAGPGGSADEILSWMEAGALDY
                     FPLPIDPRIIFGVFRRVLEKRDLRRETSLLERKLEKKYVFQGLVSKNPAMLDVFALVE
                     RISRHFSSVLLTGETGTGKELLARALFQLSGTKARQLVVCDCASIPDTLFESELFGHV
                     RGAFTGADRTKKGLFEDAHDGVIFLDEIGEIPFLIQAKLLRVLENRQFRPVGGNEVKY
                     VNVRVIAATNRDLPAMIRKGEFREDLYHRLNRIEIRVPPLREHVEDIPLLIRHFLELL
                     NKSYGKTVHGVSRDVQKLFLKYGWPGNVRELENALQSAVLVTRRDFIDLADLPKSIRE
                     PAAARRRAVFIERENLSSLEELEKEYISYVLAQTKNNLKRSAEILGVSRTTLYNKLAK
                     YGLSRG"
     gene            complement(8515..12666)
                     /locus_tag="PKV64_07600"
     CDS             complement(8515..12666)
                     /locus_tag="PKV64_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPN16980.1"
                     /translation="MPKKAVLIATLLLALAGSAAAEIGSLSHLFLLGRGLKDTDGDGL
                     ADRVAVTIILPDSPTAVELALAADISARANLESLAQNPALVKRESEIPDIAGIDSPIL
                     VGPNVKWLKQAAREGGVDLPELEAGQGFVGTFKTKTQNGIILTAGSEEALLRTGRAFF
                     LRWPYFWDVWGRETGPTYEMLERDVSAFLKTEGVSLQMTFIRSALYEFPRSGIPRGAL
                     KKLDFSAGGIKDLGLEIYFTDDGDQARSLRALESLQSSHRRGEKTGILSYAGCERLTF
                     ALRYGKSVLSASVSRLGHPRRMLTPSFKNPPRTDGAGREFDLRDVFTTKGLYGDLDGD
                     SIPDTIEGRIVAPSSEAVRGIAPLASRLVLQTAGSMFPLVYLDSEIEFRKSVVAPILV
                     GSNALTRELERVGKLSFPELENAFGIIRAVPRAFNKSSALVVAGADSLGLDRTLGYLS
                     STFPYFDERGDGRPRLDEAAADLERFFKGEKGAAEARFAPALKKTLESLKDKDLESIK
                     ADLLLPISNPAFEEELKAIAAAVAARPPLEVSSDILRGSKRIFEKERTFSWEADDALA
                     AVKARLETLAAAASENQAPVPLRISLGLSEPAEVRARLKARIEETAAEILKPTDMKVE
                     VFSAYKQGFFWLTEHVFPALRGKPVAKLTIRFAAAAEDRGKPKRFYTESARWLQELYP
                     VDEIAVRDLGLPAGAVQFEMKDPGGPVYEVTAQDGRNAVIWSGSFSPRVREMTYLKPL
                     PEWGAVQVTTGWIRLERGAEILLDEDLPTDLERLWAFYQDEGLGPVYDHVQKKTGGSP
                     AFSKQPFFKQLRVEVWASEPDFRLGLDEEAVSSLEALHDEIYFDTLDFLRGITELDPE
                     EAELPEDTSRFSAPGNVFPVIHPSTPGEPPRIKITFEDWPASVPSLAVTWKERGREAL
                     TKKIEFTEVKPSSLSLPALVYSGLEERIDRLVFDLAFEKESDYLGCLDLLASYRDLRG
                     RGLLADPLSFPRLKEIALSLKCREMAREESVPISPPAPPAAPLSVSASAGGPIVDTFK
                     ILSPEMVEDAVSRLGRYPVLHAYIAGRSYENRPVPVIEAYEPSGPNVSIPRLISMKPT
                     LYLSGRQHANEVSSTTYILKLAELLATDRAAREYIGKINFVLHPMENPDGAALAYALQ
                     NLTPFHSLHAGRYSALGLEIGTMTGARRPLLPEAAVKRDLSAKWLPDIALNLHGYPSH
                     EWVQPFSNYSPYLFRDYWIPRGWFAYVPGLTLPIYDRYKTAGEDIRAFIISEMNADPA
                     LKESNRKFYDRYERWASRWQPHLNYLEMDAGLNLYAKRRSSTELRLTPKSRMTYAEET
                     PELMDETAQGAWLDFLSTQGLAYVRAHMKYLARAKFDVVRIEEEIQDRVRIQFLRGRP
                     GTIEK"
     gene            12799..13227
                     /gene="mscL"
                     /locus_tag="PKV64_07605"
     CDS             12799..13227
                     /gene="mscL"
                     /locus_tag="PKV64_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003387862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPN16981.1"
                     /translation="MFKEFKEFALKGSLVDLAVGFIVGGAFGKIVTSLVNDILMPPLG
                     LLLGKVDFSGLFIDLSGGGYATLEAAKAAGAATLNYGAFLSTVVDFLLVALALFFVVK
                     QMNRLRRPAPAAPAAKSCPFCLSSVPEKAVRCPFCTSDLT"
     gene            complement(13220..13978)
                     /locus_tag="PKV64_07610"
     CDS             complement(13220..13978)
                     /locus_tag="PKV64_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN16982.1"
                     /translation="MNLRAQDLHFTRPGFELLVPAFSASGGEITAIVGPNGAGKTTLL
                     KCLSGLWRPSGGRVLLDGRDAAALGDSARARRLAFVPQEQAATFNFTVLEFVLMGRAA
                     ALSLFASPSTADRTIAEEALAYVGFRHPSSRPLFRLSSGERRLVLIARALAQKAEILI
                     LDEPTTFLDPRRETEVMRLLGRLASSEGKTIVVTLHDLDAAVHYAGWMVFLKDGRVVA
                     DGRTEEILSADLLGRVYDIPMDIIEHAGRRFIVK"
     gene            complement(13975..14982)
                     /locus_tag="PKV64_07615"
     CDS             complement(13975..14982)
                     /locus_tag="PKV64_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPN16983.1"
                     /translation="MSRSKRLLFLALLVSPLFVFFFSLTVGPADIPLPALGKTLLSPV
                     VEAWRGSVTPAERDILFGIRLPRILLGLAVGAALSVSGASLQAVFKNPLVNEYILGLS
                     SGAAFGASLSIVVFGSSVPPQIAAFAFALLAVGLVLLTARTPRSSPVSILLTGVIISA
                     FFSAVLSLVEFFASPYSLQTLFYWLMGNLGTAGWRDLAVPLPLIAAGTTVLILMSWRL
                     NVLSLDDEEARSLGVDVKRNRRLAVLLATLVTAAAVSVAGIIGWLGLIIPHLVRLLAG
                     VDNRRILPLSAALGASILVLADDAARSLAAFEVPVGIFTSLVGLPLFILLLSKSSKIW
                     R"
     gene            15174..>16051
                     /locus_tag="PKV64_07620"
     CDS             15174..>16051
                     /locus_tag="PKV64_07620"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M64 N-terminal domain-containing
                     protein"
                     /protein_id="HPN16984.1"
                     /translation="MKRLLGAVLIVIAAAAGFGADGPVFDRYFLDQTLRLDYYHIGDF
                     QEEIVTLDRALVQGAWAGSKTRLLDPFDLGRYSVKIIDEASGTLIFSRGFDSYFGEYR
                     TTEEAAKGIRRTYHESALIPLPKAPFLFVLESRDRNNVPRLIFEQAVDPASIEVLRKP
                     LASGVKVFDLAVSGDPHVKVDLAFIAEGYTAAEEAKLRADLDRFVELLFSQEPYRSLR
                     GKFNVRGVWKPSDDNGCDEPGYGVYKSTAVAATFDSLGSERYVLTEDNKALRDIAAHA
                     PYDTLAVMINHKRYGGG"
BASE COUNT         3032 a         4922 c         5197 g         2900 t
ORIGIN      
        1 cgggacgttt atcgccgcca gctatgagac ggtcaacttc ctcggagcgg gcgagacgct
       61 gaacctgacc gcccaatacg gcaagcggat caagaactac agcttcggct tcaccgagcc
      121 ttacctcttc gaccggccga tcagcgcggg cttcaacatc tacgaccgct acctgaatta
      181 ttatgagctc tacaaccgga agggccgggg cgtcgacttg acggtgaacg cccgccttct
      241 cggctacctc cgcggcagcc tgacgtacgg cttcgagtac gtcgacatca ccgtctccga
      301 gagctacggc tcctacaagg aatatctcga gatgtacggc atgggccatt actacatgag
      361 ctcgctgtac ccctcgatct accgctcgac catcgacagc ccgctgactc cgagctccgg
      421 gacgctctat tcggcctccc tgaaatacgc cgggaccttc ctggggggag aagtccactt
      481 atacaagccc cggctggaat tcacccatta ccagccgatc atccgcggct cctcctggca
      541 ttcgatcggc ttccatgtcg agggctccta tatcaagccg ctccgggaca cgacgacgcc
      601 gttctgggag cggtttttcc tcggcggcga gcggagcatc cgcggctacg aggtctacac
      661 gatcggcccc cggtccccgt cgggaacgct cctggggggc gaaaaaatgc tggtcatgaa
      721 cttcgaatac atctgggctc tcggcggacc gatctccctg attctcttcc acgaccgggg
      781 gaacgcctgg gccggaaatc agaaaatgag cttgaccgac gtctacacgt ccaccgggat
      841 cgaggcccgg atcttcgtcc cggccctccg cgttcctttt cgcctgattt tttcgtataa
      901 taaccgccgg atctacgatt cggattccaa gttcgctttt cggttcgcgg ttggaaccac
      961 gttttgatgg accgtcgttt ttttcattta aggaggattg tcatgcgtaa gtccgtgatt
     1021 gccgccgccc tggccgttct ctgcctcggc gcggccgcca acctttcatt cgcccaggga
     1081 aaaatcggag ttctcgactc gcagcaggtc ttggagcggt cggccgaggg gaaaaaggtc
     1141 atcgcccggc tccaggaaag cgaaaagaag aacaccgcgg ccgtcaccgc ccttgacgat
     1201 cagatccgcc agatccagac caagctcaac accgggcgcc tgacgatgac ggatgaagcc
     1261 gccctccagc tccaggccga cctggaacgc aagaccaccg agcgcaaacg ggtggccgag
     1321 gacgccgtgg cgggcatgca ggagctctcc aaccgccttt tcatgaagct ccagtccgag
     1381 ttgatgccga tcgtccaggc ggtcgggaag gaaaagggcc tggacatcat tttcgacgtc
     1441 cagaagagcg gggccgttta ctgggctccg gccgtcgatg tcaccgaaga catcatcaag
     1501 cgctacgacg cctccaaggc ctcggaaaaa taagccgccg gagaatcgcg gagtgttgaa
     1561 tatcgagaaa atcatccata ttctgcccca ccggtatccc tttttgctgg tggaccgggt
     1621 cctcgagctc gaaaaatcga agcgcatcgt cgctctgaaa aacgtcacct acaacgagga
     1681 gttcttccag ggtcattttc cggagatccg gatcatgccc ggagtcctga tcgtcgaggc
     1741 gatggctcag gccggcggga tccttttgtt ccactcgatt cccgacccgg agacgaagtt
     1801 tgtcctgttc agcaagatcg acgacatgaa attccgccgc ccggtcatcc cgggcgatca
     1861 gatccggatc gaggcccacg ttctccgcaa ccggaaccgc ttctacacgc ttcatgccaa
     1921 ggcctttgtg gaaggggaga tcgcggccga gggggacatg gtcgcctcga tgctggagcg
     1981 agaggagttt tatggccgga agtgacctcc ggatccatcc gacggccgtt gttcatcccg
     2041 gcgcccggat cgaccccggc gccgtcatcg gtcctttttg cctcatcggc ccggaagccg
     2101 tcgtcgggcc ggggacggtc ctcgaatccc acgtcacggt cggcggccgg acgaccatcg
     2161 gggcggacaa ccgcgtttcc ccgttcgtgg ccatcggcgg cgagccccag gacgtcgggt
     2221 acaaggacga gccgagccgg atccaaatcg gcgaccggaa cgtgatccgg gaattcgtga
     2281 cgatccaccg ggccacgacc aagcaggatt tggaaacggt catcggcgac gacaactatc
     2341 tcatggccta ttcccacgtc gcccacgact gccgcctcgg ccgggggatc atcatgcttc
     2401 acggggcgac gctcggcggt cattgccagg tcggcgattt cgccctggtc ggggcgatga
     2461 gcggcgtcca ccagttctgc cgcctcggcc ggttttcctt catgggcggg ggatcgatgg
     2521 tcacccagga cgtcctgccg ttctgccggg tcgccggcca gaggccgacc cgggccttgg
     2581 gcctcaacgt cgtcggcctg cggcgcaacg gattcagccg ggagcggatc tccgccctga
     2641 aggcgatctt tcaaatcctc ctgttcgaaa acctcaacac gacccaagcc ttggaacgga
     2701 tcgaggccgc attccctccg tcggaagacc gggaagaaat cgcggccttc atccggacgg
     2761 ccaagcgcgg ttacgtcaaa aaatcggcgg acgcatggga tcgcgattag gcctgatcgc
     2821 cggggcgggc gaattgtccc gggccgtctt ggcggaagcg aaaaaaagcg gcttgttctg
     2881 cgcggtcgcg ggcctgcgcg gcgcggcccc ggccggcctg gagcgggagg cggacgcctt
     2941 cgcctggttc ggcccggccg acctggacga gctggcggcc tttttccggg gccacggcat
     3001 ccgggaagcg gtgatgttcg ggaaaatcga gcatcgcctg attttcggcg aggccctcgg
     3061 aaacggagcg atccgggccc tggcggccgc cctgccggat cattcgccct cgactctcct
     3121 ccgcgccctc atcgaatacc tcggagcggc cgggatcgcg gtttgcgatc cttcgcggtt
     3181 cctcgagccg tacggctgtc ccgagggcgt cctcgtcccg gccgccgctc ccgaggccgt
     3241 tgagaccgaa accgccttcg gctggccctt ggtccgaacc ctggccgacc tggacatcgg
     3301 ccagacgctg gtcgtcaagg accgggccgt cgtcgccgtt gagggaatgg aaggcaccga
     3361 cgaaaccgtc cggcgggccg ccgccctggc cggtcccgga gtcgtcgtcc tcaaggccgc
     3421 ccggacgcgc caggatcccc gcgtcgacct gcccatggtc ggcctggaaa cggtccggac
     3481 gctggtcgaa gcccgggccg ccgccctggt cgtcgaggcc ggccgggtgc cgtttttcca
     3541 aagggaggaa gcccgggccg cggccgcggc cggaggggtg gcgctcatcg gccggcgctg
     3601 atcggaagca ttatggaaaa aatcaagctg ggaatcatcg gggtggggta cctgggcatg
     3661 cagcatgccc ggatcgcctc ctatctcgag gaggcggacc ttcgggccgt ggccgacatc
     3721 gagttcaaga aagctttcga aatcggcaac cgccacggcg ttcgttatta ccaggaattc
     3781 gaggaaatgc tggacgatat cgacgccggc atcgtcgcgg tcaacacctc gagccactac
     3841 ggcgtggcca aacgtctcct cgagaggggc aagcatgtcc tcgtcgaaaa gcccatcacc
     3901 gagaccgtcg gccaaggaga ggagctgatc gccctggccc ggaaaaacgg cctgattctc
     3961 caggtcggac acctggagcg gttcaacccg gccgtggaag ccgtcgaaaa catcatttcc
     4021 gagccgaaat tcatcgaggt tcagcggttg ggctcgttct cggcccggtc gctggacgtc
     4081 gacgtcgtcc tggacctgat ggtccacgac ctggacatca tcatggcctt gatcaaggac
     4141 gaagtctcgg tcatccggag caccggaatc catgtggtct cggacaaggt ggatatcgcc
     4201 aacgcccgcc tggaattcaa gggcggctgt gtggccactc tgacggccag ccgggttcac
     4261 cagggcaagg tgcggaaact gcggattttc gaaccgacgg cttattacgc catcgactac
     4321 atcgaccagg aagtgaagat cttccccctc agcggccggc agaccgacat caagagcttc
     4381 aagatcaaga aggaagagcc gttgaaaaag gaaatcctga atttcctggc ctgcatcgcc
     4441 gaagggcgga ccggcaaggt cagcggggag gagggcctcc aagccctccg cctcgcccac
     4501 gacgtcctca aggaaatgga gacgacctaa agccgtcccg gccgtcccct cccggcggcg
     4561 gccttctcac tgcatcttga agaacgaatc ctccagcccg gtgttgaaaa ccaccttggc
     4621 gaccgtgacc cggccgaact ccgcgcctcc ctggagaatc gtcagggaat ggggaaccat
     4681 caagccctcg acgttcttga agtcgtccat cagggtttcg acgtcgattt ccgtgcccat
     4741 cgcgtccgtc ttggaccgga tctgctcggt caggaacgtg gccgtgttga tataaagagt
     4801 ggcttggaag ccgtccttga acgtctgttc gacgaggtaa tagtccttgt ctccgacctt
     4861 ttccttgccc ttgaggacga acgtgattcc gtatttctcg ggattcaagg ccgcgtcgta
     4921 gcccatggcc tgaagccgct gggcctggct ctgcttttcg ttcatttcct cggccacgcc
     4981 ggtctgggga ttgatgaacc atcccttttc cccgtcgaag gcctgggtga tcaacatgcc
     5041 catgacgtcg atgtccatcc gcatcatgtt cggctctttc tggtaaatcg tcagggccgc
     5101 ggacatcccc atttgggtga tctcgatcgt cccggacgtg gtcgtatcct tgatcgcggc
     5161 cagggcggtc cggccgcctt gggcttcgag gctttttgaa aggatgtcgg ccaccgtcag
     5221 ttcctgggcc tggaggccga ggcagagaat ccccagggcg gcgcatacac cgatcaacaa
     5281 acgctttttc atgaatgatc tcctttcccg ccccccgccg ggctccggat tattctaccg
     5341 gaaacgacgg aaaaaaccaa tgcgccgggg gccgttttcc ggcccgggcg ccggcctgcc
     5401 gaacggggcg tttacgcttc ttcatacgtt gtgttcggcc gaaggttcag gaccgaagtc
     5461 cccggccttt ccgggaggag agaaggcgga agatcgtctc gcggccgaaa cggggatgag
     5521 cggagcggcg gctcgacgat atcctttccc gggcgtgagc cggcgtccgc ggaggacggc
     5581 ggacgggaag aaaacggcac tcccagcgtc taaaacgtgt cccgagcggg gcggacgtgt
     5641 tatcatgtaa gcttcgcgcc cgcggattta agcgaatcct tgggaccggc cgcttcttcc
     5701 aggagagaaa taaaatgaag atgaggaata tccgcaactt gaaaaggcgg gcggcctggc
     5761 tcgtatggcc ggccgccgtc ctgattctcg cggccgggct cctggccgcc gaggattcgg
     5821 acgccggcgg cccccagacg gccgcccaaa tcgccgagtg gaaaaaaagc gccccccgcg
     5881 tctatttgga cttcgaaaag ggggatccgg attacatccg gacggaaatc cccttcgtca
     5941 attatgtccg ggaccgcaag gaagccgaca tccacatcct cgtcacgacc cagactacgg
     6001 gcggcggcgg gatcgaatac gccatggcct tcctcggcca gggggatttc gcttcgcttt
     6061 ccggcgatct catctacgcc tcggcaaaga ccgacaccga ggaagaggtc cggaaaggct
     6121 atgtcgccgt cctgaaaatg gggctggtcc cgttcgtcgc ccggacgccg atccgggatc
     6181 tcctgtcgat cgagtaccgg gaaaagatcc agccgacgga cgtcgtcgat ccttggagat
     6241 tctgggtctt cagcttgagc gtcggcgggg agctggatca tgaatcgcag gcgaaatccc
     6301 aagccatgtg gctcaacgcc tcggccaaca aaatcacccc ggacatgaaa atccgcctgg
     6361 gcctctcgac ctatttcgcc cgggacgaat tccgcttcga cgaggagctc atcgaaagcg
     6421 attcccgggg gctcgacttg gccggcttgt tcaccaaaag cctcggggag cattggtcgg
     6481 ccggcgtcta cgtctccgcc ggctcgtcca cgtacgccaa tatccggacg aaaatcagcc
     6541 cggccccggt cctcgagtac aacattttcc cgtactccga gtccacccgg cgccagctcc
     6601 ggatcatgta caaggtcgga ttcgatttcg tccgctaccg ggaggagacg atctacgaca
     6661 agacgaggga gaatctgacg agtcaagcgc tctcggccgt cctggagatc aaagagccct
     6721 ggggcacgct ggaagccggg ctggagggat cgcattactt ccacgacttc aagaaaaacc
     6781 ggctggagtt caacggcgag gcggccatcc gcatcctcag gggcttggat ttcgtcgtcg
     6841 atcttcatta cgaacgggtc cgtgaccagt tgtctctggc caagggaaac cggagcctgg
     6901 aggaaatcct cctccggcgg aaagagctgg ccacggatta taatctgggg cttacggtcg
     6961 gcctgagcta cacgttcggc tcgattttca gcaacgtcgt caatccgcgc ttcggcgatc
     7021 tcggaaacct ggattaccac tcccacgatt gagaagggac gggccggccg tcatcctcgg
     7081 ctcagcccgt acttggccag cttgttgtag agggtcgtcc gggaaacgcc gaggatttca
     7141 gccgatcgct tcaggttgtt cttcgtttgg gccaggacgt aggagatgta ttccttttcc
     7201 agctcttcca ggctggaaag attctcccgt tcgatgaaca cggcccgccg ccgggcggcg
     7261 gccggctcgc ggatggactt cggcaggtcg gccaggtcga tgaaatcccg gcgggtcacc
     7321 aggaccgcgc tttggagggc gttttccagc tcccggacgt ttcccggcca gccgtatttc
     7381 aggaacagct tctgcacgtc ccggctcacg ccatggacgg ttttgccgta ggatttattg
     7441 agaagctcga ggaaatgccg gatcaggagc ggaatgtcct cgacgtgctc gcggaggggc
     7501 gggacgcgga tctcgatccg gttcaggcgg tggtagagat cctcccggaa ttcgcctttc
     7561 cggatcatcg ccggcaggtc ccggttggtg gccgcgatca cccggacgtt gacgtacttg
     7621 acctcgtttc ccccgaccgg ccggaattgg cggttttcca ggacgcggag aagcttggcc
     7681 tggatgagaa aagggatttc gccgatttcg tcgagaaaga tcactccgtc gtgagcgtcc
     7741 tcaaagaggc ccttcttggt ccggtcggcc ccggtgaacg ctccccggac atggccgaag
     7801 agctccgact cgaagagggt gtcgggaatc gaggcgcagt cgcagacgac gagctggcgg
     7861 gctttcgtcc cgctcaattg aaaaagggcc cgggccagca gttccttgcc cgttccggtt
     7921 tctccggtga gcaggacgct tgagaaatgc cgggagatcc gctcgacgag ggcgaagacg
     7981 tcgagcatgg ccggattctt ggagaccagc ccctggaaga cgtatttttt ttcgagcttc
     8041 cgctccaaca ggctcgtttc ccggcgaaga tcccgttttt cgaggacccg gcggaagacg
     8101 ccgaagataa tccgcggatc gatcggcagg gggaaatagt cgagggctcc ggcttccatc
     8161 caagacagga tctcgtcggc cgagccgccc ggtccggcca cgacgatttg aaggagcgga
     8221 tcgaagcttt tcaattcccg gagcagggcc cgggttccct ccggatcggc cgaggcgtcg
     8281 aggaggaggg aacggatggc gtgggtttgg atgaaatcga ggacgttttc gtcccggccg
     8341 caaaaaaaga ggctgatgtc cggatccaag ccggaatccc ggagccgctc ataaatcgag
     8401 gcgtctcggc cgacgaccag aaaagatatg gcggtgtcca ttttctgaga gtgtctaaaa
     8461 tattaacata aagtttttcc cggcgtcaaa cgcgccgccc gcctcccgga ctgtttattt
     8521 ttcgatcgtt cccggccggc cgcggaggaa ttggatccga acgcggtctt gaatttcctc
     8581 ctctatccgg acgacgtcga atttcgcccg ggccagatac ttcatatggg cccggacata
     8641 agccaggccc tgggtggaaa gaaaatcgag ccaggcgccc tgggccgtct cgtccatcag
     8701 ctcgggggtt tcctcggcgt aggtcatccg gctcttgggc gtcagacgga gctcggtcga
     8761 cgagcggcgc ttggcgtaga gattcaggcc ggcgtccatt tcaagatagt tcaggtgcgg
     8821 ctgccagcgc gaggcccagc gctcataccg gtcgtagaat ttccgattcg attccttgag
     8881 ggccggatcg gcgttcatct cggagatgat gaacgcccga atgtcctcgc ccgccgtctt
     8941 gtaacggtcg tagatcggaa gcgtcaggcc ggggacgtag gcgaaccagc cgcggggaat
     9001 ccagtaatcc cggaagaggt aaggggaata attcgagaac ggctggaccc attcatggga
     9061 aggatagccg tggagattga gggcgatgtc gggaagccat ttcgcgctca ggtcccgttt
     9121 gacggccgct tcgggaagga gcgggcggcg ggccccggtc atcgtcccga tttccaggcc
     9181 cagggcgctg tagcggccgg cgtggaggct gtgaaacggc gtcaggttct ggagggcgta
     9241 agccagggcg gccccgtcgg gattctccat gggatgaagg acgaagttga tctttccaat
     9301 gtattcccgg gcggctcggt cggttgccag aagctcggcc agtttcagga tgtaggtggt
     9361 cgaggagacc tcgttggcgt gctgccggcc gctcaagtag agcgtcggct tcatcgagat
     9421 caggcggggg atggagacgt tcggcccgga cggttcgtag gcctcgatga ccggaacggg
     9481 ccggttctcg taggaccgcc cggcgatgta ggcgtggagg acgggatacc ggccgagccg
     9541 ggagacggcg tcttcgacca tctcgggcga gaggatcttg aatgtgtcga cgatcggccc
     9601 gccggccgaa gcggagacgg acaagggagc cgccggcggc gcgggcgggg agatcgggac
     9661 cgattcctcc cgggccatct cccggcactt gagggagagg gcgatctcct tcagccgggg
     9721 gaagctcagc ggatcggcca gaaggccgcg gccgcggaga tcccggtacg aagccagaag
     9781 atcgaggcat ccgagataat cggattcctt ctcgaaagcg aggtcgaaga cgaggcggtc
     9841 gatccgttcc tccagcccgc tgtaaacgag ggccggaagc gaaagggagg acggcttcac
     9901 ttcggtgaat tcgattttct tcgtcagcgc ttcccggccg cgctccttcc aagtcacggc
     9961 caggctgggg acggacgccg gccagtcctc gaacgtgatc ttgatccgcg gcggctcgcc
    10021 cggcgtcgac ggatggatga ccgggaagac attccccggg gcgctgaagc gggaggtgtc
    10081 ctcgggaagc tcggcctcct cgggatccag ctcggtgatg ccccggagga agtcgagcgt
    10141 gtcgaaataa atctcgtcgt gcagcgcctc gagcgagctg acggcttcct cgtcgaggcc
    10201 gagacggaaa tcgggctcgc tggcccagac ttccactctc agctgcttga aaaacggctg
    10261 tttggagaac gccggcgaac cgccggtctt tttttggacg tggtcgtaaa ccggcccgag
    10321 accctcgtcc tggtaaaacg cccagaggcg ctcgagatcg gtcgggagat cctcgtccag
    10381 caggatttcg gccccgcgct ccagacggat ccagcccgtg gtcacctgga ccgcgcccca
    10441 ttcgggcagg ggcttgagat acgtcatttc ccggacgcgc ggggaaaacg atccgctcca
    10501 aatcaccgcg tttcggccgt cctgggccgt gacctcgtac accgggcccc ccgggtcctt
    10561 catttcgaat tgaacggctc cggcgggcag gccgagatcg cggacggcga tttcgtcgac
    10621 cgggtagagc tcctgcagcc agcgggccga ctcggtgtag aaacgcttcg gctttccccg
    10681 gtcctcggcc gcggccgcga accggatcgt cagcttggcg accggcttcc cccgcagggc
    10741 cggaaagacg tgttcggtga gccaaaaaaa accttgcttg taggccgaga aaacctcgac
    10801 cttcatgtcg gtcggcttga ggatttcggc ggccgtctcc tcgatccggg ctttcaagcg
    10861 ggcccggact tcggccggtt cgctgaggcc gagactgatg cggagcggga ccggcgcctg
    10921 gttttccgaa gcggccgcgg ccagggtttc gagccgcgct ttcaccgcgg ccagggcgtc
    10981 gtcggcttcc caggaaaacg tccgctcttt ttcgaagatc ctcttggatc cccggaggat
    11041 gtccgaggag acctccaggg gcggccgggc cgcgacggcc gcggcgatcg ccttcagctc
    11101 ttcctcgaag gccgggttgg atatcggcag aaggagatcg gccttgatcg attccaggtc
    11161 cttgtccttg agggattcga gggtcttttt caaggccggg gcgaaccggg cctcggccgc
    11221 gcccttttcc cccttgaaaa atcgttccag gtcggcggcc gcctcgtcca ggcggggccg
    11281 gccgtcgccc cgctcgtcaa aataggggaa ggtcgagctc aggtagccca gcgttcgatc
    11341 caagccgagg gaatcggccc cggccaccac cagggcggac gatttattga aggcccgggg
    11401 cacggcccgg atgatgccga aggcgttttc cagctccggg aaggacaact tgccgacgcg
    11461 ctccagctcc cgggtgaggg cgttggaccc gaccaggatc ggggcgacga cgcttttccg
    11521 gaattcgatt tcggaatcca ggtaaaccag cggaaacatc gacccggccg tctggaggac
    11581 gaggcgggag gccaagggag cgatgccccg aacggcctcg gaggaggggg cgacgatccg
    11641 gccctcgatc gtgtccggga tcgagtcgcc gtccaggtcg ccgtagaggc ccttggtcgt
    11701 gaaaacgtcg cggaggtcga attcgcgccc ggcgccgtcg gtcctcggcg gattcttgaa
    11761 tgacggcgtc agcatccgtc tcgggtgccc gagacgggaa acggaggccg acaggacgga
    11821 ttttccgtac cgcagggcaa acgtcagccg ctcgcatccg gcgtaggaga gaatcccggt
    11881 tttttccccc cggcggtggg acgattggag ggattccagg gcccgcaacg agcgggcctg
    11941 gtctccgtcg tcggtgaaat agatttcaag gcccaggtcc ttgatcccgc cggcgctgaa
    12001 atcgagcttt ttcagggccc ctcgggggat cccggagcgg gggaactcgt acaaggccga
    12061 gcggatgaac gtcatttgga gggaaacgcc ctccgtcttg agaaaggcgg agacgtcgcg
    12121 ctcgagcatc tcgtaggtcg gacccgtttc ccggccccag acgtcccaga aatagggcca
    12181 acggagaaaa aaggcccggc ccgtccggag gagagcttcc tcggacccgg ccgtcaggat
    12241 gatcccgttc tgcgtcttcg tcttaaaggt gccgacgaat ccttgtcccg cctccaactc
    12301 gggcagatcg acgccgcctt cgcgggcggc ctgtttcagc catttgacgt tcggcccgac
    12361 gagaatcggg ctgtcgatcc cggcgatatc cgggatctcg gattcccgtt tcaccagggc
    12421 cggattctgg gccaggcttt ccaggttggc ccgggcggag atatcggcgg ccagggcgag
    12481 ttcgacggcc gtcggcgaat ccgggaggat gatcgtcacg gccacccggt cggctaaacc
    12541 gtcgccgtcc gtgtccttga ggccgcggcc gaggagaaag agatgggaca ggcttccgat
    12601 ttcagccgcg gccgagccgg ccagggccag gaggagggtt gcgataagga cggctttttt
    12661 cggcatgggg gcaccttcta agaatccgcg ttttcaggtg tcttatttta gtacaaggag
    12721 accggttctc caagttgact ctccgtctcc ctcttcttta agatgattcc gggcattcgc
    12781 ggaagaaagg aggaacatat gttcaaggaa ttcaaggaat tcgccctgaa ggggagcctg
    12841 gtcgacctgg ccgtcggatt catcgtcggc ggagccttcg gcaagatcgt cacctcgctc
    12901 gtcaacgaca tcctcatgcc gccgctcggc ctgcttctgg gcaaagtcga tttctccggt
    12961 ctgttcatcg acctctcggg cggcggatac gcgacgctcg aggcggccaa ggcggccggg
    13021 gcggcgacgt tgaattacgg ggcgttcctc agcacggtgg tcgacttcct cctcgtcgcc
    13081 ctggctctgt tcttcgtcgt taagcagatg aaccggctcc gccggccggc cccggccgct
    13141 ccggcggcca agagctgccc cttctgtctt tccagtgttc cggagaaggc cgtccgctgc
    13201 ccgttctgca cgtccgatct tacttgacga tgaagcggcg gccggcgtgc tcgatgatgt
    13261 ccatggggat gtcataaacc cgaccgagga gatcagccga gaggatctct tccgtccgac
    13321 cgtcggcgac gacccgcccg tcctttaaaa aaaccatcca gccggcgtaa tggacggccg
    13381 cgtccagatc gtggagggtg acgacgatcg tttttccctc gctcgaagcc aggcggccga
    13441 ggagcctcat gacctcggtt tcccggcgcg gatcgagaaa tgtggtcggc tcgtccaaaa
    13501 tgaggatttc cgccttttga gccagggccc gggcgatcag gacgagccgc cgctcgccgc
    13561 tgctgagccg gaagaggggc cggctcgacg gatgccggaa tccgacgtac gccagggcct
    13621 cctcggcgat cgtccggtcg gccgtcgacg gcgaggcgaa aagcgagagg gcggccgccc
    13681 ggcccatcag gacgaattcg agaacggtga agttgaacgt cgcggcctgc tcttggggga
    13741 cgaaggccag gcggcgggcc cgggcggaat cgccgagggc cgccgcgtcc cggccgtcga
    13801 gaagaacccg tccgccggaa ggccgccaca gcccgctgag gcatttgagg agcgtcgtct
    13861 tgcccgctcc gttcgggccg acgatcgccg tgatctcacc gcctgaggcg gagaaggccg
    13921 gaacgaggag ctcgaagccg ggccgggtga aatggagatc ctgggcgcgg agattcatcg
    13981 ccagatcttg gaggatttcg aaagcaggag gatgaacaga ggaaggccga ccaggctggt
    14041 gaagatcccg accgggactt cgaaagcggc caggctccgg gcggcgtcgt cggccaggac
    14101 aagaatgctc gcccccaggg cggccgacag cggcagaatc cggcggttgt ccacgccggc
    14161 caggaggcgg acgaggtgcg gaatgatcaa gccgagccag ccgatgatgc cggcgacgga
    14221 aacggccgcg gccgtgacca gggtggccaa gaggacggcg aggcgccggt tgcgctttac
    14281 gtcgacgccg agcgagcggg cttcctcgtc gtccagcgaa aggacgttca agcgccagga
    14341 catgaggatc aggacggtcg ttccggccgc gatcaaggga aggggaacgg ccaggtcgcg
    14401 ccagccggcg gttccgagat tgcccatcag ccagtagaag agcgtttgga gggaataggg
    14461 gctggcgaaa aactcgacca gggaaaggac ggcagaaaaa aacgcggaga tgatgacccc
    14521 ggtcaagagg atcgagaccg gcgaggagcg cggtgtccgg gcggtcagga ggaccaggcc
    14581 gacggccaag agggcgaagg cgaaggcggc gatctgcgga gggacggagg acccgaagac
    14641 gacgatcgag agggatgccc cgaaggccgc ccccgaggag aggccgagga tgtattcgtt
    14701 gacgagcgga tttttaaaga cggcctggag cgaagccccg gagacggaca gggccgcccc
    14761 gacggccaag ccgagaagaa tccggggaag gcggatgccg aacaggatgt ccctttccgc
    14821 cggagtgacc gatccccgcc aggcttcgac gacgggcgaa agaagcgttt tgccgagggc
    14881 gggaaggggg atgtccgccg ggcccaccgt caaggaaaag aaaaagacga acaagggcga
    14941 aaccaggagg gcgagaaaga gcagccgttt cgatcgagac atggaggatt cccattatag
    15001 gcgattcgga cccgtctttc catgcgggga cggccggctt ttctgctata attgaaatcc
    15061 tctttccgtg cgcgggggat gaggcggcgg agccatgacg gcctccgcga tcctcccgcg
    15121 ccgccgggaa gagagggatc aggggaatgt cgcgtcccgg agaggaggaa ttcatgaaac
    15181 gactgcttgg agccgttctg atcgtgatcg cggccgccgc cggattcgga gcggacgggc
    15241 ccgtcttcga ccgttatttt ctcgaccaga ccctgcgcct cgattattat catatcggcg
    15301 actttcaaga ggagatcgtc acccttgacc gggccctcgt ccagggcgcc tgggccggct
    15361 cgaaaacccg cctcctcgat ccgttcgacc tgggaagata ttccgtcaag atcatcgacg
    15421 aagcctcggg gacgctcatt ttttcccgcg ggttcgacag ctatttcggc gaataccgga
    15481 cgaccgagga agccgccaag ggaatccgcc ggacgtacca tgagtcggcc ctgattccac
    15541 tcccaaaggc gccgtttctc ttcgtcctgg aaagccgcga ccggaacaac gtcccgcggc
    15601 tgattttcga acaggccgtc gatccggcct cgatcgaggt cctgaggaag ccgctcgctt
    15661 ccggcgtcaa ggtgttcgac ctggccgtca gcggcgatcc ccacgtcaag gtcgacctgg
    15721 ccttcatcgc cgaggggtac acggccgccg aggaggccaa gcttcgggcc gatctcgacc
    15781 ggttcgtcga gcttctcttc agccaggagc cgtatcgcag cctccgcggc aagttcaacg
    15841 tccggggcgt ttggaagccg tccgacgaca acggctgcga cgagccgggc tacggcgtct
    15901 acaaatccac ggccgtcgcc gcgacgttcg actcgctcgg ctcggagcgt tatgtcctga
    15961 ccgaggacaa caaggccctc cgggacatcg ccgcccacgc cccctatgac acgctggccg
    16021 tcatgatcaa ccacaaacgc tacggcggag g
//
LOCUS       DAOOXH010000057        15175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_3914_length_15175_cov_7.237632, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000057 DAOOXH010000000
VERSION     DAOOXH010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 15175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15175
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3914_length_15175_cov_7.237632"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PKV64_07625"
     CDS             <1..497
                     /locus_tag="PKV64_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16985.1"
                     /translation="WQGTVEFSLGRYNIADPLFETIYRPGGSITGLGVTANLIPHLGF
                     SLDVKVLSKNGSLSHSKEKTSFVLIPVSLGLRGSYPVAFLIPFAGLGLDYYFFLENNP
                     IGTVVDHAKGWHAAAGVYFQFGETIPILPFAKIKYTMVKASAGNRTIDLGGWEYGAGL
                     AIAF"
     gene            523..1917
                     /locus_tag="PKV64_07630"
     CDS             523..1917
                     /locus_tag="PKV64_07630"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPN16986.1"
                     /translation="MAINASIAIPKVLLLHCPGDKIFLHDYYTSYSSKANYYWPPTDL
                     VLLSGVLRETRLAVIDAVADRLSEEACLEHIRRFAPDVVVFTSGTATWKSDFAFTARV
                     KTETGARLVASGSIFFFEAERFLAAAPHVDAAILDMATPEIVDYVHGRERDYTALAVR
                     RADGLSIPAPACQAPVVTIPVPRHDLFNLRANRSPLARRRPFALVVTSIGCPYGCVFC
                     VAGAMAYRSRDLAGVLDELETLQNLGVKEIMFNDPTFTVSKKRVLELCAEMEKRGLRF
                     SWVCNAHIATVDEELAEAMKKAGCHTVMIGVESGQEEILAATDKKITREKAIRAFAVC
                     KKFRIRTLAYFIVGLPGETPDSVARTIRFAKELDPDFASFTVLTPDIGSPLRAESIAK
                     GLIDPDVLLFDSTCPPVFSSGSLSREELWRQRQKAVRSFYLQPAYLLKQLGAVRNLRD
                     FLYLLDQARTMFFR"
     gene            1978..4377
                     /locus_tag="PKV64_07635"
     CDS             1978..4377
                     /locus_tag="PKV64_07635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPN16987.1"
                     /translation="MPTSSKSSGTAVLLVLLILAAAVRFWGIGWGLPYTYHADENFFA
                     AKSMRFLEGDLNPHFFHVPTLHMYALAGMWKVYYGIEKAAGNIRTNAEFEETYTQFPQ
                     VFYLIGRFLSALMGAGTVLLLYLLGKRMFGPRAGALSALLLTFGLEHARMSHDMLPDV
                     PMVFFLVLSFLFIWNIAEKGRTRDYLWAGVAAGLAMTTKYGGALLFIPLFIAHAYRVR
                     ETKRPAWEYILSPRLIGAGLAFLAVFILGSPYVVLDFPKFFKDFRWQSSHLTEEGHFG
                     DSTSHSALLFYLRYGFIENVGIVSQFLVLGGFLLGVFRRQKRDVILLSYPLVQFTLIS
                     LWGARATRYLMPLAPFFCLIGGIFLAWLLDWSRRKWVAEGADRKRRTAFTAVSWLLVA
                     AIIAPSAVRLAKLDSALAGLDSRTRAKQWIEYNLPKGTVIALESYGPPISRQDYTVYY
                     NHTFSRTDLEFLSFKGIEYAVVSDMMAGRFTRFPQDFPREAAVYEELAREGTLVKTIE
                     PGFKEPLFEIHMPKLEIYKLSRAPAQGFPVNFRTYVQDVSLSRAPSGSWEIRSAVSAG
                     GGLSVKEDVSNPYVKISDSGGRELARLILRPGRLPSAEPWAAEASETVADIPEGAVID
                     FGYEYLPAPRTTSYTSEESLRKEYRLPGVMDAAGRKAGRWSGRVWFAAVPGPGGYHFQ
                     TVAAVFREDGVFVRSRVFGPALRYGNGRIDHPYVLFTDAEGSEAKKITVFSGRLGAFG
                     SDGRGPAETSILLPGLPPRFRLFIGHEDGAARPNSDIPTGPYRLELPPLPGPPGPESQ
                     I"
     gene            4396..6714
                     /locus_tag="PKV64_07640"
     CDS             4396..6714
                     /locus_tag="PKV64_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPN16988.1"
                     /translation="MSKARRRKTNTQTASKAAPRRRFPLSEAVGILLVFAALFSLAAL
                     ISYNSLDPSWASAAPGNARIDNWGGRIGAETAETAYAALGLAAWLLPLAAGYLGIRAI
                     LHGNRRIIFGKAGQFILLLLILCPLLDLFFQNIAWGGKKIPVGGVLGQLLVSFLHGFF
                     GSPGTLIFLLAAASLFAVAVGGFSFKKLFLGVGRMTRFTVAGVKIRIPRRPKPEKPAR
                     EKKASARAKAEPAAAPAPARAERREPSPPPRRELRKAAIKPPLPPVARPEQYAFPQFD
                     DRPDYTLPPLTLLDPGAPTEQIDRKELQEKRSLIEEKLAEFKVEGEVREYHPGPVITT
                     YEYSPKPGIKINQVTNLSDDLALALGAESVRIQRIPGKSSLGVEIPNNKREIIKLRDI
                     LESEAFIKSPSKLTLALGKTVHDEVYVTDLGVMPHLLVAGATGTGKSVCLNALIASIL
                     YKASPEEVKLLLIDPKRLEFSLYDGIPHLLSPVINDAKKAAAILMDTVKKMEERYYLM
                     QQAKVRNIDQYNHHIADQLREKRGKLTEEEQRKLRPMPYIVIIIDELADLIMVSGQDV
                     DFAIGRLAQLARAVGIHLVLATQRPSTDIITGTIKNNFPSRIAFRVPSKIDSRVILDE
                     NGAEKLLGMGDMLFIPPNYPRLVRLHCSYVSIPEVRRLVNFVKKQGTPDYDERIVDVL
                     QRTGEMPWGESDEKDEMFEEAVKLILHTGQASASYLQRRLKLGYARASRLIDQMEQEG
                     ILGPSEGSKPREILVDVKSYMLNSAKKNPAGD"
     gene            6721..7257
                     /locus_tag="PKV64_07645"
     CDS             6721..7257
                     /locus_tag="PKV64_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPN16989.1"
                     /translation="MKPPPKAEVDLEAVVAKYRPVVGFKIRRAVGWDNPDWEDLTNEV
                     LTQTIAAVRAGEFRGDSQIGTFIYTIACRRIVDHIRGKSKIARRFPAENDPASSQDRL
                     EEHERIRGLAAAVSALPPKYREVLDLYYYQELSREETALRLGLAPARVSERVHYALKL
                     LRNQMRRAAKSFFPPSPD"
     gene            7281..7904
                     /locus_tag="PKV64_07650"
     CDS             7281..7904
                     /locus_tag="PKV64_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPN16990.1"
                     /translation="MRRPEHLEFERQIDDYLLGRLSAEEAECFEKHYFDCPACFQRTA
                     ERAALLEAVRLAGPASAAAVRSAKKRARLRFGLAWAAAGTAVLLAAAVFLLVPRTPRP
                     PDFPDSGTRIVRGAPVVLIAPAGILEENPKSFSWRGVDGAAEYRITVEGLEPVWTAET
                     DKPSVVIPAEIAPRFLPGKTYSWTVKAYTAEGTLLAVSPAAAFSIGR"
     gene            complement(7920..8600)
                     /gene="ricT"
                     /locus_tag="PKV64_07655"
     CDS             complement(7920..8600)
                     /gene="ricT"
                     /locus_tag="PKV64_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPN16991.1"
                     /translation="MSDLICAVSLTTGKTIRARKGETELVRGDLCLVESEFGGDLARV
                     VETDGPVCRHPNRASEAPNVLRKADAEDERRLAWLREREQRAFAFCLDRIKFRNLAMK
                     LTAVRFYFSEKKGVFFYTADGRIDFRQLVKDLAKELRMRIEMRQVGVRDEAKMIGGLG
                     VCGRPLCCASFMKTFEPVTIQKARKQQIAINPTKISGLCGRLMCCLAFEEESRGRMYY
                     DEDIPNED"
     gene            complement(8605..9699)
                     /locus_tag="PKV64_07660"
     CDS             complement(8605..9699)
                     /locus_tag="PKV64_07660"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16992.1"
                     /translation="MAFDDIPGNAPLKAILRSALRRKSALPSLLFCGAAGAGQRETAL
                     ELAKAFNCLHLDDDACGECPSCRSIAAGNHPDIIEVINLPRKQKKTAADGGRENGAAA
                     GDEDAAEDAVAEPAGSGSKPRNIVIDQMRRAIELALMKPMIGRKRVFLVDDASEMEAA
                     AANASLKILEEPPAFSQFILVAGNPDVLLPTIRSRCQTLVFGPVGTKEIIAVLRARGL
                     DETRAVSVAAVVRGDYQRALNTDWDEFFEERGRAWDMFRAILTGSDGGDFIRRHAGGR
                     KAASREDIETLLGFFRGFGRDLLVLGETAGGGPLFNPDLEERLGELASVVPPERALRL
                     IRAVDAAQVSLEKYANVRLLASVLFARMTG"
     gene            complement(9707..12223)
                     /gene="uvrA"
                     /locus_tag="PKV64_07665"
     CDS             complement(9707..12223)
                     /gene="uvrA"
                     /locus_tag="PKV64_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPN16993.1"
                     /translation="MLNEIVVKKAAQHNLKRIDVRLPRNRFIVVTGPSGSGKSSLAFD
                     TLFAEGQRRYIECMSAYARQYMEMMEKPDVESIEGLSPAISIGQKTISSNPRSTVGTV
                     TEMYDLLRLLFARAGIPHCPSCGRAVSSQSTDQIIDRILDSARGRIKVMAPVIKGRKG
                     EYHRLFDRLRKKGYLRLRLDGVFRDLEDEIILVKTRSHTGEVQIDDLRPSASSRGRLQ
                     EAVERALELTDGEVLIIAEDGAESYYSRKLRCPVCDVSLPDLEPRNFSFNSPYGACPR
                     CHGLGFDTTLNEDGETELTGDRCPSCEGARLNPRSLAVRIDGRNIHDMSGLSIDRLIA
                     DLGRLEVLVSREAVAERIRRELLGRLRVCHELGLSYLTLDRTAASLSGGEAQRVRLAA
                     QVGARLRGILYVLDEPTIGLHQRDNGRLIGMIRRLRDDGNTVLVVEHDEQTIRAADYI
                     LDLGPGAGEQGGAVVAEGTLPEILKSPASLTAKYLRGEAAVSFPDGRRPAKSFLTIRN
                     AREHNLKNIDVSIPLGCMTAVTGVSGSGKSTLVFDILHRALQNALFRAKLRTGAFDKL
                     EGVQHLDKVISVDQKPIGRTPRSNPATYAGLFTDLRLLFSLSQEARTRGFGPGRFSFN
                     IPGGRCEDCQGAGVKRVEMHFLPDVFVTCDRCGGLRYNKETLAVRYKGRSIADFLAMT
                     VDEAYEYLKAQPRLKRRLATLRQVGLGYIRLGQPAPTLSGGEAQRIKLTKELGRRATG
                     RTLYLLDEPTTGLHFDDVRKLLEILSELVRMGNTVVVIEHNLEVIRFCDYIIDLGPEG
                     GEDGGRVVAAGSPEDVAAADTPTAAFLRSALSAPGRTGTN"
     gene            12304..14649
                     /locus_tag="PKV64_07670"
     CDS             12304..14649
                     /locus_tag="PKV64_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPN16994.1"
                     /translation="MGRQENLHKALEILRDESLKTNSLTALKHLPAQEGRYADYPAEV
                     HPALVKALRDKGFERLYVHQRASWEAVRNGGNPVVVTPTASGKTLCYNLPVLDAILKD
                     PSARALYLFPTKALANDQRAELDETIARLPGEIRIFTYDGDTPQDARRAIRARGHIVL
                     TNPDMLHAGILPHHTKWIKLFENLKYVVIDELHNYRGIFGSHLANVLRRLKRVAAFYG
                     ANPQFILCTATIANPLEMAEKMIEAPPTLIDDNGAPRGEKYVLFHVPPVVNAALGIRR
                     SYVNESRRIASIFLHQNLQTIVFAQSRLVTEVLLTYLKEDLEKTIKQEGLIRGYRGGY
                     LPVKRREIEKGLREGKILGVVSTNALELGIDIGTLDVAVLASYPGTIASTWQRIGRAG
                     RKSGASAAVLVASSAPLDQFIIHNPDYFFGKSVERALINPDNLTILINHLECAAFELP
                     FQDGEKFGRAEIGEILKYLEEEKFLHHSKDKWYWTSDAYPADGVSLRSISSDNFVVVD
                     TTEKTRVIAEVDFTAALTTLHEKAIYLCEGEQYFVEKLDFTERKAYVKKTDIDYYTDA
                     IDYTKIKILDEFDKKRLKRFDVRHGEVHVATQVVGFKKIKFHTQENVGAGDLSLPQNE
                     MGTTAWWATIPGDLLAGLAYEPEEKINGLYGLSYILHNVAPLFLMCDIHDLGVSIGDN
                     ATGESIPTRDVRAKVQQGAGVLSLEPDFEPNIFLYDNYPGGIGLSPTLFDLDEKLLDY
                     CLKTIAACPCKSGCPSCVGPVYESGESAKETALAILTKMLA"
     gene            14732..>15175
                     /locus_tag="PKV64_07675"
     CDS             14732..>15175
                     /locus_tag="PKV64_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPN16995.1"
                     /translation="MNIFKLITDASIVVQAILLILLFFSVFSWTIIIFKRKTFRTATA
                     RSKKFLEIFKKSRSLTEVNEAAKSYPGSPAAALFLAGYKEIAYLAKQQQAASEPANGS
                     RLENLSRALTKASNAEVARMEKMMGFLATTGSVSPFIGLFGTVWGI"
BASE COUNT         2741 a         5061 c         4569 g         2804 t
ORIGIN      
        1 cctggcaggg gacggtcgaa ttttccctcg gccggtacaa catcgccgac ccgctgttcg
       61 agacgattta ccggccgggc ggatcgatca ccggcctcgg cgtgacggcc aacctgatcc
      121 cccaccttgg tttttcactc gatgtcaagg tgttgtctaa aaacggttcc ctgtctcata
      181 gcaaggaaaa gacgtcgttc gtcctcatcc cggtgtctct cgggctccgg ggctcctatc
      241 cggtggcttt cctgattccg ttcgccggcc tcggcctcga ttattacttc tttttagaga
      301 acaatcccat cgggaccgtc gtcgatcacg ccaagggctg gcacgccgcc gccggcgtct
      361 acttccaatt cggagagacg atccccatcc tccctttcgc caagatcaag tacacgatgg
      421 tcaaagcctc ggccggcaat aggaccatcg acctgggcgg atgggaatac ggcgccgggc
      481 tggcgatcgc cttttagccg gcggcggtga agggatcgga gcatggccat taacgcatcg
      541 attgcgattc ccaaggttct ccttctccat tgcccgggcg acaagatctt cctccacgac
      601 tattacacca gctactcgtc caaggccaac tactattggc cgccgaccga tctcgtcctc
      661 ctctccggcg tcctgcggga aacccggctc gcggtgatcg acgcggtggc cgaccgtctc
      721 tctgaagaag cctgtcttga gcacatccgg cggttcgctc cggacgtggt cgtcttcacg
      781 agcgggaccg cgacgtggaa gagcgatttt gctttcaccg cccgggtcaa gacggaaacc
      841 ggggcccgcc tcgtcgcttc gggttccatc ttcttcttcg aggcggagcg gttcctggcc
      901 gccgcccccc acgtcgacgc cgccatcctg gacatggcca ccccggaaat cgtcgactat
      961 gtccacgggc gagagcggga ttataccgcc ctggccgtcc ggcgggcgga cgggctgtcc
     1021 atcccggccc cggcgtgtca ggcgcccgtt gtcaccatcc ccgttccgcg ccacgacctc
     1081 ttcaatctcc gggccaaccg cagtccgctg gcccggcgcc ggccgttcgc cctcgtcgtc
     1141 acctcgatcg gctgcccgta cggctgcgtt ttctgcgtgg ccggggccat ggcctaccgg
     1201 tcccgggacc tcgccggtgt tctagacgag ctggaaaccc tccaaaatct cggcgtcaag
     1261 gaaatcatgt tcaatgaccc gaccttcacc gtctcgaaga aacgggtcct ggaactctgc
     1321 gccgagatgg aaaagcgcgg cctccggttt tcctgggtct gcaacgccca tatcgccacc
     1381 gtggacgagg aactggccga agccatgaag aaggccgggt gtcatacggt catgatcggc
     1441 gtggaaagcg gccaggagga aatcctcgcg gccacggaca agaaaatcac ccgggaaaag
     1501 gcgatccggg cgtttgccgt atgtaaaaaa ttccgcatcc ggacgctcgc ttatttcatc
     1561 gtcggactgc ccggagagac gcccgattcg gtcgcccgga cgatccgatt cgccaaggaa
     1621 ctggaccccg acttcgcctc gttcacagtc ctgacgcccg acatcggctc gcccttgcgg
     1681 gcggaaagca tcgccaaggg gctgatcgat ccggacgtcc tgctgttcga cagcacctgt
     1741 ccgccggttt tcagctcggg ctccctgtca cgggaggagc tctggcggca gcggcagaaa
     1801 gccgtccggt ccttttacct ccagccggcc taccttctca agcaactggg agcggtccgg
     1861 aacctccggg atttcctgta tctcctcgat caggcccgga ccatgttttt ccgctgagac
     1921 ccgggccggc ccggtgattg acgtttccgg aaatcttccg ctatattaca atccatcatg
     1981 cccacttctt caaaatcttc cgggacggcg gttctccttg ttctcctcat tctggccgcc
     2041 gcggtccggt tctggggcat cggctggggc cttccttaca catatcatgc ggacgaaaac
     2101 ttcttcgccg caaaatcgat gcgcttttta gaaggggacc tcaatcccca tttctttcac
     2161 gtccccacgc tccacatgta cgccctggcc gggatgtgga aagtctatta cggtatcgag
     2221 aaagccgccg gaaatatccg gaccaacgcc gaattcgaag agacctacac gcaattcccc
     2281 caggttttct acctgatcgg acgttttctt tctgccctga tgggcgccgg gacggtcctt
     2341 cttctttacc ttctcgggaa aaggatgttc ggaccgcggg ccggggcgct gtcggctctt
     2401 ctgctcacgt ttgggctgga gcacgcccgc atgtcgcatg acatgctgcc cgacgtcccc
     2461 atggtctttt tcctagtcct gtccttcctc ttcatctgga atatcgcgga aaaagggcgg
     2521 acgcgggatt atctctgggc cggcgtggcg gccggcctgg cgatgaccac aaagtacggg
     2581 ggagcgcttc tgttcatccc ccttttcatc gctcacgcgt accgggtccg cgaaaccaag
     2641 cggccagcct gggaatacat cctcagccct cgcctgatcg gcgcgggtct cgcttttctg
     2701 gccgtgttca tcctcggcag cccctatgtc gttctcgatt ttcctaaatt tttcaaagat
     2761 tttcggtggc agagcagtca cctgacggag gagggacatt tcggagattc cacctcccac
     2821 tcggctcttc ttttctacct gcgctatgga ttcatcgaga atgtggggat cgtctcccaa
     2881 ttcctcgtcc tcggcggttt ccttctcggc gtgttccggc gtcaaaaacg ggacgtgatc
     2941 ctgctttcct atcccctggt ccagttcacc ctgatttctc tctggggagc gcgggcgacg
     3001 cgatacctga tgcccctggc cccgtttttc tgcctgatcg gcgggatctt cctggcttgg
     3061 cttctggact ggagccggag gaaatgggtg gccgaaggag cggaccggaa gcggcggacc
     3121 gccttcaccg ccgtctcttg gctcctggtc gcggcgatca ttgctccctc ggccgtccgc
     3181 ctcgcgaagc tggactcggc cttggccggc ctcgattccc ggacacgggc caagcaatgg
     3241 atcgaataca accttcccaa gggcacggtc atcgcccttg aatcctacgg ccccccgatt
     3301 tcacggcagg actacaccgt ctattataac catactttca gccggacgga cctggaattc
     3361 ctttccttca aggggattga atacgccgtc gtcagcgata tgatggccgg acgcttcacc
     3421 cggttccccc aggacttccc cagggaagcc gcggtttacg aggagttggc ccgggagggc
     3481 accctggtga agacgatcga gcctggattc aaggagccct tgtttgaaat ccacatgccg
     3541 aaacttgaaa tctacaagct cagccgggct cccgcccagg gcttcccggt caattttcgg
     3601 acctatgtcc aggacgtttc gctaagccgc gcgccgtcgg gttcctggga gatccggagc
     3661 gcggtttcgg ccggaggcgg cctgagcgtc aaggaggacg tctcgaatcc ctacgtgaaa
     3721 atctcggact ccggcggccg ggaattggcc cgcctcatcc tccgccccgg ccggcttccc
     3781 tcggccgagc cgtgggccgc cgaagcgtcg gaaaccgtcg ccgacattcc cgagggggcg
     3841 gtcatcgact tcgggtacga atatcttccg gctccgcgga cgacgtccta tacgtccgag
     3901 gaatccctgc gcaaggaata ccgcttgccg ggggtgatgg acgcggcggg gcggaaagcc
     3961 gggcgttggt ccggacgagt ctggttcgcg gccgtccccg gccccggggg ctatcatttc
     4021 cagaccgtcg cggccgtctt cagggaggac ggggttttcg tccggagtcg cgttttcggt
     4081 ccggcccttc gatacggcaa cggccggatc gatcatcctt atgtcctctt caccgacgcg
     4141 gagggaagcg aagcgaagaa aatcacggtt ttctccggcc gcctcggcgc gttcggctcc
     4201 gacgggcggg gaccggccga gacctcgatt cttcttcccg gcctccctcc ccgtttccgg
     4261 ctgttcatcg gccatgaaga cggagccgcc cggccgaact ccgatatccc gaccggtccg
     4321 taccgcctcg agctgccgcc gctccccggc ccccccgggc cggaatccca aatatgatat
     4381 aattccggca ggagcatgag taaggcgagg cggcggaaga cgaacactca aaccgcgtca
     4441 aaagcggccc cccggcggcg gtttcccctt tccgaggccg tcggcatatt gctcgtcttc
     4501 gccgctcttt tttccctggc cgccctgatc agctataatt ccctcgaccc gtcttgggcc
     4561 agcgccgcgc ccgggaacgc ccgcatcgac aactgggggg gaaggatcgg ggcggagacg
     4621 gccgaaacgg catatgccgc cctcggcctg gccgcctggc tcctcccctt ggccgccgga
     4681 tacctgggca tccgggccat tcttcacggc aaccggagaa tcatcttcgg caaagccggg
     4741 cagtttattc tcctgcttct catcctctgt ccccttttgg atcttttctt tcaaaacatc
     4801 gcttggggcg gaaagaaaat cccggtcggc ggcgttctcg gccagcttct ggtctccttc
     4861 ctccacggtt ttttcggcag tccgggaact ttgatcttcc tcctggcggc ggcttcgctt
     4921 ttcgccgtcg ccgtcggcgg tttttccttc aagaaattgt ttctgggcgt cggccggatg
     4981 acccggttca ccgtcgccgg agtaaagatc cgcatccccc gccgccccaa gccggaaaag
     5041 ccggcccggg aaaagaaggc ttcggcccgg gccaaagcgg agccggccgc cgccccggct
     5101 ccggcccggg cggagcgccg cgagccgtct cctcctccgc ggcgcgagct gcgcaaggcg
     5161 gcgatcaaac cgccgcttcc tcccgtcgcc cggcccgagc agtatgcgtt cccccagttc
     5221 gacgaccggc ccgactacac cctcccgccg ctgacgctcc tggatcccgg cgctccgacc
     5281 gagcagatcg acagaaagga gcttcaggaa aaacgaagcc tgatcgagga gaagctggcc
     5341 gagttcaagg tcgagggcga agtgcgggaa taccatcccg gcccggtcat cacgacctac
     5401 gagtactctc cgaagccggg aatcaagatc aaccaggtca cgaatctctc cgacgacctg
     5461 gccctggccc tcggggccga atccgtccgc atccagcgga ttccgggcaa gtcctccctg
     5521 ggcgtggaaa tccccaacaa caagcgggag atcatcaagc tccgcgatat cctcgagtcg
     5581 gaagcgttca tcaaatcgcc gtccaagctg acccttgcct tgggcaagac cgtccatgat
     5641 gaagtctacg tgacggacct cggagtcatg ccgcacctgc tggtcgccgg ggcgaccgga
     5701 accgggaaaa gcgtttgtct caacgccttg atcgccagca tcctctataa ggcctcgccc
     5761 gaggaggtca agctcctgct catcgatccc aagcggctgg agttttccct ttatgacgga
     5821 atcccccatc ttctcagccc ggtcatcaac gacgccaaga aagccgcggc catcctgatg
     5881 gacacggtca aaaagatgga ggagcgctat tacctgatgc agcaggccaa ggtccgcaac
     5941 atcgaccagt acaaccatca catcgccgac cagctccggg aaaaaagagg caagttgacc
     6001 gaggaggagc agcggaagct ccggcccatg ccctacatcg tcatcatcat cgacgagctg
     6061 gccgatctca tcatggtcag cggtcaggac gtcgacttcg ccatcggccg gctggcccag
     6121 ttggcccggg cggtcggcat ccacctggtc ctggccaccc agcgtccctc gaccgacatc
     6181 atcaccggga cgatcaagaa caacttcccc agccggatcg ctttccgcgt ccccagcaag
     6241 atcgactccc gggtcatcct ggacgaaaac ggggcggaaa agctcctggg gatgggcgac
     6301 atgcttttca tccccccgaa ttatccccgg ctcgtccgcc tccactgctc ctatgtctcg
     6361 attccggaag tccgccggct ggtcaacttc gtgaaaaagc aggggacgcc ggactacgac
     6421 gagcgcatcg tcgacgtcct ccagcgcacc ggggagatgc cttggggcga atcggacgaa
     6481 aaggacgaaa tgttcgagga ggcggtcaag ctgattctcc acaccggaca ggcctcggct
     6541 tcttatctcc agcggaggct caagctcggc tatgcccggg cctcccgcct gatcgaccag
     6601 atggagcagg agggcattct cgggccgtcg gaaggcagca aaccccggga gatcctggtc
     6661 gacgtgaaaa gctacatgct gaactcggcg aaaaaaaatc cggccggtga ttaggcgatc
     6721 gtgaagcccc cgcccaaggc cgaggtcgac ctggaagccg tcgtcgcgaa ataccggccg
     6781 gtcgtcggtt tcaagatccg ccgggccgtc ggctgggata acccggactg ggaggacctg
     6841 accaacgagg ttctcacgca aacgatcgcg gccgttcggg ccggggaatt ccgcggggat
     6901 tcccaaatcg gcacgttcat ctacaccatc gcctgccggc ggatcgtcga ccacatccgg
     6961 gggaaatcga aaatcgcccg ccgattcccg gcggaaaacg acccggcctc ctcccaggac
     7021 cggttggagg aacacgagcg gatccgcggc ctggccgcgg ccgtctcggc cctgccgccg
     7081 aaataccgcg aggtcctgga tctctactac tatcaagagc tgagccggga ggaaaccgcc
     7141 ctccgcctcg ggctcgcccc ggcccgggtc agcgaacgcg tccactacgc cctcaagctc
     7201 cttcggaacc agatgcggcg ggcggcaaag tcattttttc ccccgtcgcc cgactaatga
     7261 aaggacaagg attcgatccg atgcgccgcc ccgaacacct cgaattcgaa cgacagatcg
     7321 acgactatct tctgggccgg ctgagcgcgg aggaagccga gtgtttcgag aagcactatt
     7381 tcgactgccc ggcctgtttt cagcggacgg ccgagcgggc cgctcttctc gaggccgtcc
     7441 ggctggccgg gccggcttcc gcggcggccg tccggtcggc gaaaaaacgg gctcgcctca
     7501 ggttcggtct cgcctgggcg gcggccggga cggccgtcct cttggccgcg gccgttttcc
     7561 tcctggttcc ccgaacgccc cgcccgccgg attttccgga cagcgggacg cggatcgtcc
     7621 gcggcgctcc cgtcgttctc atcgcccccg cggggattct cgaggaaaat ccgaaatctt
     7681 tctcctggcg cggcgtcgac ggggcggcgg aatacaggat tacggtcgag gggctcgagc
     7741 ctgtctggac ggcggaaacg gacaaaccgt cggtcgtgat cccggcggaa atcgcccccc
     7801 gcttcctccc gggaaaaacc tactcctgga cggtgaaggc ttacacggcc gaggggacgc
     7861 ttctggccgt ctcgccggcg gcggctttct cgatcggccg ctgaccgcgg gccggcggct
     7921 caatcctcgt tcggaatatc ctcgtcgtag tacatccggc cccggctctc ctcctcgaag
     7981 gccaggcagc acatcagccg gccgcacaat cccgagatct tcgtcggatt gatggcgatc
     8041 tgctgtttgc gggctttctg gatcgtcacc ggctcgaacg ttttcatgaa cgaggcgcag
     8101 cagagcggac ggccgcagac gccgaggccg ccgatcatct tggcctcgtc tcggacgccg
     8161 acctggcgca tctcgatccg catccggagc tccttggcca ggtccttgac cagctggcgg
     8221 aaatcgatcc ggccgtcggc cgtgtagaag aacacgcctt ttttctcgct gaaatagaac
     8281 cggaccgcgg tcagcttcat ggccagattc cggaacttga tccggtcgag gcagaaagcg
     8341 aaggcccgtt gctcgcgttc ccggagccag gccagcctcc gctcatcctc ggcgtcggct
     8401 ttgcggagga cgttcggcgc ttccgaagcc cggttcggat ggcggcatac cggcccgtcc
     8461 gtctcgacga cccgggccag gtcgcccccg aactcggatt ccacgaggca gagatcgccc
     8521 cggaccaact cggtctcgcc cttgcgggcc cggatcgtct ttcccgtcgt caaggaaacc
     8581 gcgcaaatca aatcgctcat gtcgttatcc tgtcattctc gcgaaaagaa cgctcgcaag
     8641 caagcggacg ttcgcgtatt tttccaaaga gacctgggcc gcgtcgacgg cccggatcag
     8701 ccgcaaggcc cgttccggcg ggacgaccga ggcaagctcc ccaaggcgct cttcaaggtc
     8761 gggattgaat aaagggcctc ctcccgccgt ttcgcccagg acgagaagat cccgtccgaa
     8821 tccgcggaag aaaccgagca aggtctcgat gtcctcccgg gaggcggctt ttcgtccgcc
     8881 ggcatgccgg cggatgaaat ccccgccgtc gcttccggtc aaaatcgccc ggaacatgtc
     8941 ccacgcccgg ccgcgctcct cgaagaactc gtcccagtcg gtgttcaggg cccgctgata
     9001 atcgccccgg acgacggccg cgacggagac ggcccgcgtt tcatccaagc cgcgcgcccg
     9061 gaggacggcg atgatctcct tggttccgac cggtccgaag acaagcgtct ggcaacggga
     9121 ccgaatcgtc gggagaagaa cgtccggatt gcccgccacc aggatgaact gggagaacgc
     9181 cggcggctcc tcgagaatct tcagcgaggc gttggccgcc gcggcttcca tttccgaagc
     9241 gtcgtcgacg agaaacaccc gcttgcggcc gatcatcggc ttcatgaggg ccagctcgat
     9301 cgcccggcgc atttgatcga tgacgatgtt ccggggcttc gagccggatc ccgccggttc
     9361 cgcgacggcg tcttccgcgg cgtcctcatc ccccgcggcc gctccgtttt cacggcctcc
     9421 gtcggccgcc gtttttttct gtttccgcgg aaggttgatc acctcgatga tgtcgggatg
     9481 gtttccggcg gcgatcgacc ggcaggaggg acactcgccg caggcgtcat cgtcgagatg
     9541 gaggcaattg aacgccttgg ccagctccaa ggcggtttcc cgctggccgg cgccggcggc
     9601 cccgcagaac aacaggctcg ggagggcgct tttccgccgc agggcggagc ggagaatcgc
     9661 cttgagcggg gcgttgccgg ggatatcgtc gaaagccatg atccgcctaa tttgtcccgg
     9721 ttcgtccggg ggccgaaagg gcggaccgga gaaacgcggc cgtgggcgtg tcggccgcgg
     9781 cgacgtcctc gggggatccg gccgcgacga cgcgccctcc gtcctccccg ccctcgggac
     9841 ccaggtcgat aatataatcg cagaaccgga tcacttcaag gttgtgctcg atgacgacca
     9901 cggtgttgcc catccggaca agctcggaga ggatctccaa aagcttgcgg acgtcgtcga
     9961 aatggagtcc ggtcgtcggc tcgtccaaaa ggtacagcgt tcggccggtc gcccgccggc
    10021 ccagctcctt ggtcagcttg atgcgctggg cttcgccgcc ggacagcgtc ggggccggct
    10081 ggccgagccg gatgtagccg agcccgacct gccgcaaggt cgccagccgg cgcttgaggc
    10141 gcggctgggc cttgaggtac tcatacgcct catccaccgt catggccagg aaatcggcga
    10201 tcgaccgccc cttgtaccgg acggccagcg tttccttgtt gtagcgaagt ccgccgcagc
    10261 ggtcgcaggt gacgaaaacg tcgggaagga aatgcatctc gacccgtttc accccggccc
    10321 cctggcagtc ctcgcagcgg ccgcccggaa tgttgaagct gaaccgcccc ggcccgaagc
    10381 cgcgggtccg ggcctcctgg gacaaggaga agaggagccg gaggtcggtg aacaggccgg
    10441 cgtacgtggc cgggttggac cgcggcgtcc ggccgatcgg tttctggtcc acggagatga
    10501 ccttgtccag gtgctggacg ccctcgagct tgtcgaacgc cccggttctg agcttcgccc
    10561 ggaacagggc gttctgcagg gcgcggtgga ggatgtcgaa gaccagggtg cttttgcccg
    10621 aacccgaaac gccggtcacg gcggtcatgc atcccagggg gatggagaca tcgatgttct
    10681 tgaggttgtg ctcccgggcg ttgcggatcg tcaggaagct tttagccggc cggcgcccgt
    10741 cggggaagga aaccgccgcc tccccccgca ggtacttggc cgtcaaggag gccggggatt
    10801 tcagaatttc cgggagcgtt ccttcggcca cgaccgcgcc cccttgctcg ccggcgcccg
    10861 ggccgaggtc gaggatgtag tcggccgccc ggatggtctg ttcgtcgtgc tcgacgacga
    10921 ggacggtgtt gccgtcgtcc cggagccggc ggatcatgcc gatcaaccgg ccgttgtccc
    10981 tctggtggag gccgatcgtc ggctcgtcga gaacgtagag gattccccgg agccgggctc
    11041 cgacctgggc cgccaggcgg acgcgctggg cctctccgcc ggaaagcgag gccgccgtcc
    11101 ggtcgagcgt caggtaggac aagccgagct cgtgacagac gcgcaggcgg ccgaggagct
    11161 cgcgccggat ccgctcggcc accgcctcgc gggaaacgag gacctcgagt cggccgaggt
    11221 cggcgatcag ccggtcgatc gaaagcccgc tcatgtcgtg gatgttccgc ccgtcgatcc
    11281 ggacggccag gctccgggga ttgaggcggg ctccttcgca ggaggggcag cggtccccgg
    11341 tcagctcggt ctcgccgtcc tcgttcaggg tcgtgtcaaa tccaaggccg tggcaccgcg
    11401 ggcaggcgcc gtaggggctg ttgaacgaga agttgcgcgg ttcgagatcc ggcaggctga
    11461 cgtcgcagac cgggcagcgg agcttccggg aataatagct ttcggctccg tcctcggcga
    11521 tgatcaggac ctcgccgtcc gtcaattcca acgcccgctc aaccgcctcc tggagacggc
    11581 cgcgggaaga ggcggacggg cgcaggtcgt cgatctggac ttccccggtg tgtgaccgcg
    11641 tttttaccag gatgatttca tcctcgagat cgcggaaaac gccgtccagc cggagccgga
    11701 gataaccctt tttccggagg cggtcaaaga gccggtggta ttcgcccttg cggcccttga
    11761 tgacgggggc catgaccttg atccggccgc gggccgaatc gaggatgcgg tcgatgattt
    11821 ggtcggtcga ctgggagctc accgcccgtc cgcaggacgg gcagtggggg atgccggccc
    11881 gagcgaacag cagccggagg aggtcgtaca tctcggtcac ggtcccgacg gtcgagcggg
    11941 ggttggagga gattgtcttc tgcccgatcg aaatggccgg ggacaggccc tcgatggatt
    12001 cgacgtccgg tttttccatc atctccatgt attggcgggc gtaagccgac atgcactcga
    12061 tgtagcggcg ctggccctcg gcgaacagcg tgtcgaaggc caacgaggat ttgcccgatc
    12121 cggacggccc ggtgacgacg atgaaccggt tgcggggaag acggacgtcg atcctcttta
    12181 aattgtgctg ggccgccttc ttcacgacga tttcgttgag cataaccttg tcattttaag
    12241 acttttcagc ggccggagca agaaaatttg acggccggcc ggccgatgcg ctagcatgcc
    12301 gctatgggcc gccaagagaa tcttcacaag gcgctcgaga ttctccggga cgaatccctg
    12361 aaaacgaatt cgctgaccgc cctgaagcat ctgccggcgc aggaaggccg gtacgccgat
    12421 tacccggccg aggtccaccc cgccctggta aaggctctcc gggacaaggg cttcgagcgg
    12481 ctgtacgtcc accagcgggc cagctgggaa gcggtgagga acggggggaa tcccgtggtc
    12541 gtcaccccga ccgcctcggg gaaaaccctc tgctacaacc tgcccgtcct cgatgcgatc
    12601 ctcaaggatc cctcggcccg ggccctctac ctcttcccga ccaaagccct ggccaacgac
    12661 cagcgggccg agctcgacga gacgatcgcc cgcctgcccg gggaaatccg gatcttcact
    12721 tacgacggcg acactcccca agacgcccgg cgggccatcc gggcccgggg tcacatcgtc
    12781 ctgaccaatc cggacatgct ccacgccggg atcctgcccc atcacaccaa gtggatcaag
    12841 ctcttcgaaa acctgaaata cgtggtcatc gacgagctcc ataactaccg gggcatcttc
    12901 ggcagccacc tggccaacgt cctccgccgg ctcaagcggg tcgccgcctt ctacggggcg
    12961 aatccccagt tcatcctctg cacggccacg atcgccaacc cgctggagat ggccgagaaa
    13021 atgatcgagg ccccgccgac gctcatcgac gacaacggcg ccccccgcgg ggaaaaatac
    13081 gttctcttcc acgttccgcc ggtggtcaat gcggccttgg gcatccgccg gtcctacgtc
    13141 aacgaaagcc gccgcatcgc ctcgattttc ctccatcaga acctccagac gatcgtcttc
    13201 gcccagagcc gtctcgtcac cgaggtcctt ctgacctatc tcaaggagga tctggaaaaa
    13261 accatcaagc aggaaggcct gatccgcggt taccgcggcg gctacctgcc ggtcaagcgc
    13321 cgggagatcg agaagggcct ccgggaggga aagatcctcg gcgtcgtctc gaccaacgcc
    13381 ctcgagctcg ggatcgacat cggcaccctc gacgtggccg tgctggcctc ctacccggga
    13441 acgatcgcct cgacctggca gcggatcggc cgggccggcc ggaaatccgg agcctcagcg
    13501 gccgtgctgg tcgcctcctc cgccccgctc gaccagttca tcatccataa ccccgactat
    13561 ttcttcggaa agtcggtcga gcgggccctc atcaatcccg acaacctgac tatcctcatc
    13621 aaccacctcg aatgcgccgc cttcgagctc ccgttccagg acggggagaa gttcggccgg
    13681 gccgaaatcg gcgaaatcct caaatacctc gaggaggaaa agttcctcca tcattccaag
    13741 gacaagtggt actggacgtc ggacgcgtac ccggccgacg gagtcagcct ccggtcgatc
    13801 tcctccgaca acttcgtggt cgtcgacacc acggaaaaga cgcgggtgat cgccgaggtg
    13861 gatttcacgg cggccctgac gactctccac gagaaagcga tctatctctg cgagggcgag
    13921 cagtacttcg tcgagaagct cgacttcacc gagcgcaagg cgtacgtcaa gaagaccgac
    13981 atcgactact ataccgacgc catcgactac accaagatca agatcctgga cgagttcgac
    14041 aaaaaacgcc tgaaacgctt cgacgtccgc cacggcgaag tccacgtcgc cacccaagtc
    14101 gtcggtttca agaaaatcaa gtttcacacc caggaaaacg tcggcgcggg cgatttgagc
    14161 ctcccccaga acgagatggg cacgacagcc tggtgggcga cgatcccggg ggatctcctg
    14221 gccggcctgg cctacgaacc cgaggaaaaa atcaacgggc tctacggctt gtcctacatc
    14281 ctccacaacg tcgctccgct ctttctcatg tgcgacatcc acgacttggg cgtctcgatc
    14341 ggcgacaacg cgacgggcga atcgatcccg accagggacg tccgggccaa agttcagcag
    14401 ggggcgggcg tcctgtccct cgagccggac ttcgagccca atattttcct ctacgataat
    14461 tatcccggcg gcatcggcct gagcccgacg ctgttcgatc tcgacgagaa gctcctcgac
    14521 tactgcctga agacgatcgc cgcctgcccc tgcaagtccg ggtgcccgtc ctgcgtcggt
    14581 ccggtctatg aaagcgggga atcggccaag gagacggccc tggcgattct tacaaagatg
    14641 ctggcctgac cgccgtcgcc agaaagaccg gatccctggc ggcctaaatt gaatcggttc
    14701 cggccctgtg ttatcctgaa ccggaacgcc catgaacatc ttcaagctca tcaccgatgc
    14761 ctcgatcgtc gttcaggcga tcctcctcat tctcctcttt ttttccgtgt tctcctggac
    14821 gatcatcata ttcaagcgca agaccttccg gacggccacg gcccggtcca agaaattcct
    14881 ggagatcttt aaaaaaagcc ggagcctgac cgaggtcaac gaggccgcta aatcctatcc
    14941 gggcagcccc gcggccgccc tgtttctggc cggctacaag gagatcgcct acctggccaa
    15001 gcagcagcag gcggcgtccg agcccgcaaa cggctcccgc ctggaaaacc tcagccgggc
    15061 cttgacgaag gcctccaacg ccgaagtcgc ccggatggaa aaaatgatgg gatttttagc
    15121 cacgaccgga agcgtctcgc ccttcatcgg cctgttcggc acggtctggg gcatc
//
LOCUS       DAOOXH010000058        15090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_3942_length_15090_cov_6.691121, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000058 DAOOXH010000000
VERSION     DAOOXH010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 15090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15090
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3942_length_15090_cov_6.691121"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PKV64_07680"
     CDS             <1..553
                     /locus_tag="PKV64_07680"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HPN16996.1"
                     /translation="RLMPLVVEAFDWTHGVFMGARTGSETTAAAAGAVGVLRRDPFAM
                     LPFCGYNMGDYFRHWMNTGALMRNPPKIFAVNWFRVDENGKFVWPGFGDNMRVLKWII
                     DRVDGKVGARETPIGLVPDMNDFNMEGLPIPRSKMDRLFEIKPSEWKGEAGEIKKFFN
                     GFGRTIPFELRTNLEKMERALKL"
     gene            complement(586..1767)
                     /locus_tag="PKV64_07685"
     CDS             complement(586..1767)
                     /locus_tag="PKV64_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HPN16997.1"
                     /translation="MTGLREAEKIYSFPVYPKRDLVLVKGENAAVFDENGREYIDCVA
                     GNGSLNVGHSNPAVVKAVKDQAEKIISCANIFYNDARAAFLEKLISVMPAGLRKAFLC
                     NSGAEAVEAAVKFSRLATKRKKFVSTVNGFHGRTLGALSATHNSKYREDFLPLLDGFS
                     FVPYNDFEKMERAVDLETAGVIVETVQGEGGVYPGRPDYFARLRRLCTERGALLIIDE
                     VQTGFCRTGKFFGFEHFGVAPDILCLAKSIAGGLPMGAVAASDQIEVGIGKHGSTFGG
                     NPLCCAAGTAAIEFMLENKLWLEAERKGSYLMERLRLIKSSKIREVRGLGLMIGIELK
                     EKAKPYVLDLLERGLLVLPSGLNIIRLLPPLTIPRDDIDRVIERIAASLGDKEKSFPL
                     D"
     gene            complement(1764..2555)
                     /locus_tag="PKV64_07690"
     CDS             complement(1764..2555)
                     /locus_tag="PKV64_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPN16998.1"
                     /translation="MIIFKIGGGKDINREGIAKGLSLVHEPFLIVHGANGLRDELALK
                     TGYDIKRITSVSGYSSVFSDPGLIDLMMMAYAGLANKRWIETLQRHGINAVGLTGLDG
                     RLIAGKRNAGIRMRKDSKILMVRDYSGKPSGINAGLLKMLLDRGYVPVLTVPIIDENG
                     YAVNSENDDITALLAEELKADQIVMFIEEKGYLRDPSDPDSAMERISKSELAFFVGNA
                     SGRIKRKLHALLKIAGREGARAVISDGRGENPVWDALNNRGTVIS"
     gene            complement(2558..3205)
                     /locus_tag="PKV64_07695"
     CDS             complement(2558..3205)
                     /locus_tag="PKV64_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN16999.1"
                     /translation="MTMCRILVVKSDKEFEISRHLERFAALCRRSKEYQGHGWGLAVP
                     DGGRWKVYKSLAPVWEQKMRGFGRSRLLMVHARSAFRNENINLEYNMPFDSGRLAFAF
                     NGELRGVRIGGRGVSGAEKIFHFVKRLGDTGLPRPLKSGGDIIAERTAYVRAMNMVSC
                     DMSKIAVYSRFSEDEEYFSMRIKRENGTFLLCSEKYPDDEESCWERVPNHSYLEA"
     gene            complement(3202..4245)
                     /gene="argC"
                     /locus_tag="PKV64_07700"
     CDS             complement(3202..4245)
                     /gene="argC"
                     /locus_tag="PKV64_07700"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPN17000.1"
                     /translation="MGTIRAAIVGASGYAGGELLRLLLFHENVEVVRATSERNAGLPV
                     TRRHPNLRKITGLCFSSSSELNECDLIFLCLPHGEGIKNIDHFMNQAPKIIDLSADFR
                     LKRSEDRVKWYGSGHPRPELVNQFVYGIPELHREEMKKASYISNAGCNATVSILALYP
                     LFKQGAVEKGKTVIEVKAGSSEGGRSPSEYSHHPERSGCVRTYQPTSHRHKAEIIQEL
                     GLGENDVHFSATSIDMVRGILATCHVFLEEKLGEKEIWKIFREHYAGEPFIRIVKERS
                     GGYRYPEPKILAGTNFCDIGFEQDPDSNRLVVISAIDNLMKGAAGQAVQAMNIMCGFP
                     ENRGLEFCGLHPA"
     gene            complement(4248..5093)
                     /gene="lysX"
                     /locus_tag="PKV64_07705"
     CDS             complement(4248..5093)
                     /gene="lysX"
                     /locus_tag="PKV64_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HPN17001.1"
                     /translation="MRIGFLHSLIRKDEKLLLDEFSKRRGLDVVLLDDRDLVYTLGGK
                     NHRFDVVVERCLSHSRALYAIRLFEAAGIKCVNAYAVAATCGDKLMTSIALAEAGITQ
                     PEVKIAFTRESALKAIEDMGYPVVLKPAVGSWGRLLSKVNDRDAAEALLEHKAVLGSY
                     HHSIFYIQKYVEKNGRDIRSFVVGGTCVAAIHRSSPHWITNTARGGAATNCPVTPELA
                     DLSVRAAAAVGGGIVAIDVFESGSGYLINEVNHTMEFKNSLEPTGVNIPALIADYVIE
                     RGRGN"
     gene            complement(5053..6249)
                     /locus_tag="PKV64_07710"
     CDS             complement(5053..6249)
                     /locus_tag="PKV64_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015205931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HPN17002.1"
                     /translation="MVKILDSTLREGEQTPGVYFDIHVKLAIAGILDELGVDFIEAGH
                     PAVTEDIAKAVTLISAGGLKSKIAAHSRSMRSDIDKALECRADFIGIFYCVSDRRLQD
                     YSTNLVSAVDKITSVIRYAKEKRPSLIIRYTPEDTVRSKYENVLSAAKAAVSAGADII
                     SVADTTGYMIPGTERSMYEFISRLKDDLGKDGLAPFLEVHCHNDRGLAAANSLDAIRA
                     GAHIVDAAVLGLGERAGITDLGTLLAVLSFDFGLEGKWNTKILPEIYATVSRYSNVPV
                     PVNLPATGKNAFTHCAGVHTQAAIKNPLHYESFDPGRFGRASTIALDHMSGISSLQYN
                     LEKIGEPADDREWLNAVLQKVKEVGKKGRTVDLEELKMIVKYQKELSGGIENENRVSS
                     FAYQKG"
     gene            complement(6251..6427)
                     /gene="lysW"
                     /locus_tag="PKV64_07715"
     CDS             complement(6251..6427)
                     /gene="lysW"
                     /locus_tag="PKV64_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HPN17003.1"
                     /translation="MSELKTECPVCGALMILLSGTIEGELFPCRECGSQMEVVSLDPV
                     VVAEAAAEEEDWGQ"
     gene            6572..7189
                     /locus_tag="PKV64_07720"
     CDS             6572..7189
                     /locus_tag="PKV64_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPN17004.1"
                     /translation="MSTPFLEEGLRSGIINLSSLARKILPEIKKSLEKNVTESAMIMA
                     LKRYSDGIRGRAQKGSFEVEILDLTVRSNLAEYTFAASENIIKKQEKLIHDVSHQSDK
                     FLTFTRGIYEVTLIISEEFRKNVDEIFKGERKISSFSNLSAVILRLHPKTVSTPGVHY
                     SILKQLAWSNINVIEVVSTYTEFTVIIDNARIDTAFSVLKNLMMR"
     gene            complement(7382..8161)
                     /locus_tag="PKV64_07725"
     CDS             complement(7382..8161)
                     /locus_tag="PKV64_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN17005.1"
                     /translation="MKNKGREECPRCFDTGWIQVETKAGPAARRCSCHEAKTESAVLL
                     RTRIPTRYRDCTLANFEVHDDSHKDALRISRKFISNFPAQDIGLLFIGPCGVGKTHLA
                     TAILAELVTKKRAAGVFYDFRDLIRDIQATFAPDSDVSESDVLRPIFESDVLVLDELG
                     AQRSSTWVEETIFYIINRRYNERRLTIFTSNYPDTSETADDRAPMFKKTAGFGATRKP
                     EDTLVDRIGVRLRSRIYEMCKVVEISGQDYRKVAKQAGYRF"
     gene            complement(8177..9022)
                     /locus_tag="PKV64_07730"
     CDS             complement(8177..9022)
                     /locus_tag="PKV64_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPN17006.1"
                     /translation="MTSYKDFTESRNLRRYLEQIGRFPRLTEEQERELAERIHAGDAK
                     ALERMIEGNLRFVVSYVKKYKGMGLDMLDLINEGNLGLIEAAKRFDPGRNVKFISYAV
                     WWIRQAVIHSLTASARVTHVPQKISDQMLLMKRKVAQLSAELGREPNREEIAAAMKMT
                     VSEIEDLALLENKEISLNDRFDEDGQGLEEKIGDPISPSVEYQIIKASILNQVREILS
                     ELDEKEATVLKLRFGLDDDRPLTLQEIGDRLHLTRERIRQIEQTAMTKLSRSHKLQEL
                     RGYLN"
     gene            complement(9019..9489)
                     /locus_tag="PKV64_07735"
     CDS             complement(9019..9489)
                     /locus_tag="PKV64_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPN17007.1"
                     /translation="MNKTKPRPADRKAETAGPRRPRPKAGEAGCPGSSYYHISSVADR
                     FHIHPQTLRLYEREGLLKPSRSCGNTRLYSDEDLKRLEIILNLVRDLGVNLAGVEVIL
                     NMRAKIERIEAEVQAFMDSFQREFFSGREEEFEARRQSLVHVSSSQIVKVDKSE"
     gene            complement(9501..10628)
                     /gene="dnaJ"
                     /locus_tag="PKV64_07740"
     CDS             complement(9501..10628)
                     /gene="dnaJ"
                     /locus_tag="PKV64_07740"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPN17008.1"
                     /translation="MARDYYEILGVGRSASDAEIKKAYRRLARKHHPDLNPNDKAAEA
                     KFKEISAAYEVLSDPKKKARYDQFGFAGERPPADGGPGGGDYASGGFEGFDFSDFGGG
                     SFRDLFQNIFGGAAARAAAAAGPERGEDLLYTMKVGFEDAVRGVQTKIRLNRRAACPA
                     CGGSGSKSGEAKTCPTCRGSGKAFQQRGYMKFSGVCPDCGGRGTVAGRDCGDCRGQGT
                     REITETINVRIPAGVDTGSKVRIPRKGHAGLGGGPPGDLYIQIETLPHPLFRREGMSL
                     HVKVPITVPEATLGAKIDVPTLDGPAVIRIPPGTKSGQRFRLKGKGAPQPGARIRGDE
                     FVEVMIVPPPFGDERVRDLMKKLEPLYGGSPRAGMNEEVPR"
     gene            complement(10734..12662)
                     /gene="dnaK"
                     /locus_tag="PKV64_07745"
     CDS             complement(10734..12662)
                     /gene="dnaK"
                     /locus_tag="PKV64_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPN17009.1"
                     /translation="MAKIIGIDLGTTNSVVAIMEGDQATVISNEEGGRTTPSVVGFTA
                     KGERLVGQVAKRQRVTNPENTVYSIKRFMGRRYAEVPNEIKQVPFRITEAPNGDVRIA
                     VFDKQYSPPEISAMVLQKLKKAAEDYLGEKVEKAVITVPAYFNDSQRKATKDAGQIAG
                     LDVVRIINEPTAAALAYGMEKKKDQTIAVYDFGGGTFDISILEVGEGVVEVKSTNGDT
                     HLGGDDIDQRIQDWITTEFKKETGIDLAKDKMAIQRLKEAAEKAKIELSTTMETEINL
                     PFVTADASGPKHLLMKMTRAKLEALIEDLIQRSVAPCKQALSDAGLKPEDIHEVILVG
                     GQTRMPRIQALVQQVFGREPHKGVNPDEVVSVGAAIQGAVLSGDVKDVLLLDVTPLTL
                     GIETLGGVFTRMISRNTTIPTKKTEVFSTASDSQPSVEIHILQGEREMASDNRTLGRF
                     HLDGIPPAPRGIPKIEVTFDIDANGILHVSAKDMGTGKQQQITITGHSGLDEKEIKRM
                     VSDAEAHSTEDKKRRETIDARNQADQMIYSLEKILRENADKIPAAEAEAVRSEIEATK
                     KAVESDSPEAIKKALESLNRASHKMTEVLYRSAGPQPGAGAASDGPEQASSGPSGGNG
                     GSEGDVIDAEVVDDGKSN"
     gene            complement(12861..13517)
                     /locus_tag="PKV64_07750"
     CDS             complement(12861..13517)
                     /locus_tag="PKV64_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17010.1"
                     /translation="MKTRRILGLVAGTALLVAGAAGQEAAAPSPRKAARVEQEYGLAR
                     TPSTYFIFDFEAGKVSLKARGLVLKEWPIFKVKTWGRHGGVMTCPLLKKTAYNAPKRK
                     DVTPGKAEDETDKKPTAKNDDLDILELVKMPVNFTLELPGDIRMNVRLRKSGFARILD
                     GAVRLFSRGIGRSVKTIVRGIGRRPFTEIEIIFLEESAAKNIYWSFFEGQKCILYWPE
                     "
     gene            complement(13519..14163)
                     /locus_tag="PKV64_07755"
     CDS             complement(13519..14163)
                     /locus_tag="PKV64_07755"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPN17011.1"
                     /translation="MSAKIRKILLIAAAGFILAAGLIVLAANIIARSWARSEERAEIP
                     PPVEAKTLAAKTRQLESRMKSLLPRGTYIMIDTAENRLRVIRDGKELLLAIVSCGSGD
                     ILQDPSGERKWVFDTPRGEFTVSSKLVNPTWIKPDWAFYEEGEDLPKNFSDRIDNDTL
                     GDYALGIGSGYFIHGTLYTRMLGRNVTHGCVRVGDDDLKKVYQSVPMGAKVYIY"
     gene            complement(14153..>15090)
                     /locus_tag="PKV64_07760"
     CDS             complement(14153..>15090)
                     /locus_tag="PKV64_07760"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPN17012.1"
                     /translation="LEERRRTDRGGGSPVRLVPGLFPDRGRSQKLVAEGEALLRRIDD
                     DKAARAKTFALSAETLAARIESLHRITQYFNENDDIRKNASQAEIKLVEARMLVSKEK
                     YEPASAAAAAGEVFLERAEEAAVRLLERYLDSGQLAKWRRWAEEAVKESQDGDGVAIL
                     VNKVERRLTVYEKGKVRARYDIGLGKYGLSDKRRAGDEATPEGRYKVVKKIPASKFYK
                     ALLIDYPNEDDKRFFAEAKRRGQIPSHAGIGGAIEIHGGGKDSLTKGCVGLEDKDMDD
                     IYAWSVVGTPVTIVGATDVENTILDEIRKFKKNVR"
BASE COUNT         2767 a         4619 c         4332 g         3372 t
ORIGIN      
        1 ccgcctcatg cccctggtgg tcgaggcctt cgactggacg cacggcgtct tcatgggagc
       61 ccggaccggc tcggaaacca cggccgccgc ggccggggcg gtcggcgtcc ttcgccgcga
      121 cccgttcgcc atgctcccct tctgcggcta caacatgggc gactatttcc gccactggat
      181 gaacaccggg gccttgatgc gcaatccgcc gaagatcttc gccgtcaatt ggttccgggt
      241 ggacgagaac ggcaagttcg tctggcccgg cttcggggac aacatgcggg tcctcaagtg
      301 gatcatcgac cgggtggacg gcaaggtcgg ggcgcgggaa acgcccatcg gcctggtccc
      361 cgacatgaat gatttcaaca tggaaggcct gccgatcccc cgctccaaaa tggaccggct
      421 gttcgagatc aagccgtccg aatggaaggg ggaagcgggc gagatcaaga agtttttcaa
      481 cggcttcggc cggacgatcc ccttcgagct tcggacgaat ctcgagaaga tggagcgggc
      541 cctgaagctc tgattccggc cgggggtcga tcgatccggc ggcattcaat caagcgggaa
      601 gcttttttct ttatccccga gagaggcggc gatccgctca atcaccctat cgatatcgtc
      661 gcgaggaatc gtcagcggcg ggagaagcct gatgatgttg agacctgaag ggaggacgag
      721 gagccccctt tccagaaggt ccaacacata aggcttcgct ttttccttga gctcgatgcc
      781 gatcatgagg ccgagccctc ttacttccct gattttggag gatttgatga ggcgcagtct
      841 ttccatcagg tacgagcctt ttctttcggc ttcaagccaa agcttatttt cgagcatgaa
      901 ctcgatggcg gccgttccgg ccgcgcagca aagaggattg ccgccgaacg tgctgccgtg
      961 cttcccgata ccgacctcga tttgatccga ggccgcgacg gctcccatcg gaaggccgcc
     1021 ggcgatcgat ttcgccaggc agagaatgtc gggagcaacg ccgaagtgtt caaagccgaa
     1081 gaactttccc gttcgacaga agcccgtctg gacttcgtcg atgatcaaaa gagcgcctcg
     1141 ctccgtgcac agccggcgga gccgcgcgaa ataatccgga cgtcccggat aaacccctcc
     1201 ctccccctgc accgtctcga cgatcacccc ggcggtttcc aggtcgacgg cgcgttccat
     1261 tttttcgaaa tcgttgtacg ggacgaagga aaaaccgtcg agaaggggga ggaaatcctc
     1321 ccggtacttg ctgttatggg tcgccgacag cgcgcccaga gtccttccat gaaatccgtt
     1381 gacggtcgag acgaactttt tccttttggt cgcgagcctc gagaacttga ccgccgcctc
     1441 gaccgcctcg gcgccggaat tgcagaggaa ggccttgcga agccctgcgg gcatgacgga
     1501 aatcaatttt tcaagaaaag ccgcgcgggc gtcgttataa aatatattcg cgcaggagat
     1561 gattttttcc gcctggtctt tcaccgcctt gacaacggcc gggttggaat ggccgacgtt
     1621 gagcgagccg ttccccgcga cgcaatcgat atactccctg ccgttctcgt caaaaaccgc
     1681 cgcgttttcc cctttgacca gcactaaatc ccgtttgggg taaacgggga aggaataaat
     1741 cttttcggct tcccttaaac cggtcatgag atcacggtcc ctcgattgtt gagagcgtcc
     1801 caaaccgggt tttcgcccct tccgtcggaa atcacggccc gggccccctc ccggccggcg
     1861 attttcaaaa gggcatgaag cttccttttg atcctcccgg aggcgtttcc gacaaaaaaa
     1921 gccagctcgc ttttggatat cctttccatc gccgaatcgg ggtcggacgg atcccggaga
     1981 taaccctttt cctcgatgaa catgacgatt tgatccgcct tcagctcttc cgccagaagc
     2041 gccgtgatgt cgtcgttttc agaattcacg gcatagccgt tctcgtcgat gatcggcacg
     2101 gtgaggaccg gcacatagcc ccggtcgagg agcatcttga gaagaccggc gttgatcccg
     2161 gacggttttc cggaataatc cctcaccatc aggattttgg aatcttttct catcctgatg
     2221 ccggcgttcc ttttcccggc gatcagccgg ccgtccaggc ccgtaagccc gaccgcgttg
     2281 atcccgtgcc gctgaagagt ctcaatccac cttttgttcg ccagcccggc ataggccatc
     2341 atcatcaagt cgatcagccc ggggtcggaa aagacgcttg aatagcccga gacggacgtg
     2401 atcctcttga tgtcgtatcc cgtcttcagc gccagctcgt cccgcagacc gttggccccg
     2461 tggacgatga gaaacggttc gtgaaccagg ctcagtccct tcgcgatccc ctcccggttg
     2521 atgtccttcc ctccgccgat tttaaaaatg atcatttcta ggcttcaagg taagaatggt
     2581 tgggaaccct ttcccaacac gattcctcgt catccggata cttttctgaa cataaaagaa
     2641 acgtgccgtt ttcccttttg atcctcatcg agaaatactc ttcgtcttcg ctgaaacggg
     2701 aataaacggc gattttgctc atgtcgcagg aaaccatgtt catcgcccgg acataagcgg
     2761 ttcgttcggc gatgatgtct ccgccgcttt tcaggggccg cggaagcccg gtgtcgccga
     2821 gccgtttgac aaaatgaaat atcttttccg ccccgctgac gccccggccg ccgattcgga
     2881 cgccccggag ctctccattg aaagcgaacg ccaggcggcc cgaatcaaac ggcatgttat
     2941 actcaaggtt tatgttttca ttcctgaacg cgcttcgggc gtggaccatg agaagccggc
     3001 tccggccgaa gccgcgcatt ttttgctccc agaccggcgc gaggctcttg taaaccttcc
     3061 accgtccgcc gtccggaacc gctaagcccc agccgtggcc ctggtattcc ttgcttctcc
     3121 ggcaaagagc cgcaaaccgt tccaggtggc gggaaatctc gaattccttg tcggatttta
     3181 caaccaggat cctgcacatg gtcacgccgg gtgaagtccg cagaattcga gtccccggtt
     3241 ctcgggaaat ccgcacatga tgttcatcgc ctggacggcc tgcccggccg ctcctttcat
     3301 gaggttgtcg atcgcgctga tcaccaccag ccggtttgaa tccggatctt gttcaaatcc
     3361 gatgtcgcag aaattggtgc cggcgagaat tttcggctcc ggataccggt aaccgccgct
     3421 tctctccttg acgatccgga taaacggttc gccggcgtag tgctctctga agatcttcca
     3481 gatttccttt tccccgagct tttcctctaa aaagacgtgg cacgtggcga gaattcccct
     3541 gaccatgtcg atcgacgtcg ccgaaaaatg gacatcgttt tctccaagcc ccagctcctg
     3601 aatgatttca gccttgtgcc gatgggacgt gggttgataa gtacgcacgc agccgcttct
     3661 ttcgggatgg tgcgagtatt cgcttgggga gcggccgcct tcgcttgagc cggccttcac
     3721 ctcgatcacc gtttttccct tttcgactgc gccctgtttg aagagaggat agagagcaag
     3781 gatcgaaacc gtggcgttgc agccggcgtt ggaaatatag ctcgcttttt tcatctcctc
     3841 ccggtgaagc tcggggattc catacacgaa ctgattcaca agctcgggcc gcggatggcc
     3901 cgagccgtac catttcaccc tgtcctcact cctcttcagc ctgaaatcgg cgcttaaatc
     3961 gatgattttc ggggcctgat tcataaaatg gtcgatgttc ttgatccctt ctccgtgcgg
     4021 caggcacaag aaaatcagat cgcactcgtt cagttcgctc gatgaggaaa aacagaggcc
     4081 ggtgatcttc cgaagattgg gatgacgcct cgtcaccggc agacccgcgt tcctttccga
     4141 cgtcgcgcgg acgacttcga cgttctcgtg aaaaagaaga agcctcaaaa gctcgccgcc
     4201 ggcgtatccc gaagccccca cgatcgcggc ccttatggtt cccatcgtca gttcccccgt
     4261 ccccgctcga tgacataatc ggcgatcaac gccgggatgt tcactcccgt aggttcaagg
     4321 ctgtttttga attccatcgt atggttcact tcgttgatca agtagcccga gccgctttcg
     4381 aatacatcga tcgcgacaat gcccccgccc acggccgcgg cggcccggac ggagaggtcc
     4441 gcgagctccg gcgtcacggg gcaattcgtc gccgctccgc cccgtgccgt gttggtgatc
     4501 caatgcggag aactccggtg gatggcggcg acgcatgttc cgccgaccac gaagctccgg
     4561 atgtctcttc cgtttttttc gacatatttc tggatgtaga agatcgaatg atggtaagag
     4621 ccgaggaccg ctttgtgctc gagcagggct tcggccgcgt cccggtcgtt cactttcgac
     4681 agaagccttc cccaggaacc gaccgccggc ttcaggacga ccggataccc catgtcctcg
     4741 atggctttga gggccgattc acgggtgaac gcgatcttga cctccggctg ggtgattccg
     4801 gcttccgcca gggcgatcga tgtcattagt ttgtcgccgc acgtcgccgc gaccgcgtaa
     4861 gcattcacgc atttgattcc cgccgcctcg aaaagccgga ttgcgtaaag cgccctcgaa
     4921 tggctgaggc atcgttcaac gacgacgtcg aagcgatggt tttttccccc gagggtataa
     4981 acgaggtccc ggtcgtcgag aaggacgacg tcgaggccgc gccgtttcga gaattcatcc
     5041 aataagagct tttcatcctt tctgataagc gaatgaagaa acccgattct cattttcgat
     5101 tcctccggat aattctttct gatatttcac gatcattttc agttcttcaa gatccacggt
     5161 tcttcctttt ttccccacct ccttcacttt ctgaaggacg gcgttcagcc attcccggtc
     5221 gtcggcgggc tcgccgattt tttcgaggtt gtactggaga gaggaaatcc ccgacatgtg
     5281 atcgagggcg atcgttgaag cgcggccgaa gcggccggga tcgaagcttt cataatgaag
     5341 cggattctta atggccgcct gggtgtgcac tccggcgcaa tgggtgaagg cgttctttcc
     5401 ggtggcggga aggttgaccg gcacgggaac attcgaatat cttgaaaccg tcgcgtaaat
     5461 ctccggcagg atctttgtgt tccattttcc ctcaaggccg aaatcaaacg acagaaccgc
     5521 aagaagcgtc ccgaggtccg tgatgccggc ccgctcgccg aggccgagaa ccgccgcgtc
     5581 cacgatatgg gcccccgccc tgatcgcgtc gagggaattc gccgcggcca ggccgcggtc
     5641 gttatggcag tgcacctcca ggaacggagc caagccgtcc ttcccgagat cgtctttcaa
     5701 cctcgaaatg aactcgtaca tgctccgttc ggtgccggga atcatgtagc ccgtcgtatc
     5761 cgccacgctg ataatatcgg cccccgccga gacggccgcc ttggccgcgg aaagaacgtt
     5821 ttcatatttc gacctgaccg tgtcttcggg ggtatacctg atgatgaggg acggcctttt
     5881 ttccttggca tatcggatga cgcttgtgat tttatccacg gccgaaacaa gattcgtgga
     5941 ataatcctga agccgccggt cggatacaca gtaaaatatt ccgatgaaat ccgcgcggca
     6001 ttcgagcgct ttatcgatgt ccgatctcat cgaacgcgaa tgcgcggcga ttttcgactt
     6061 cagccccccg gccgaaatga gggtgacggc tttcgcgata tcctcggtga cggcggggtg
     6121 gccggcttca atgaaatcga cgcccagctc gtcaaggatc ccggcgatcg ccagcttgac
     6181 atgaatatcg aagtacaccc cgggcgtctg ttcgccttct cgaagagtcg agtcgagaat
     6241 tttcaccatg tcactggccc cagtcttctt cctcggcggc cgcttcggcc accacgacag
     6301 gatcaaggct cacgacttcc atttgagagc cgcactcccg gcaagggaaa agttctccct
     6361 cgatggtccc gctcaaaagg atcatcaacg ccccgcaaac cgggcattcc gttttcaatt
     6421 ccgacatcgt tctcctccaa ttcaatttga gacggcatta tggagaacga ggcgccccat
     6481 tgtcaaaaat atataaatta tttactatta ttattattct tgttatattt ttgacatgat
     6541 aaccatatcg gaagaaatcc atagaatcgt catgagtact ccttttttgg aggaaggtct
     6601 tcggagcggg atcatcaacc tgtcctcttt ggccaggaaa atccttcccg aaataaaaaa
     6661 aagcctcgag aagaatgtca ccgaaagcgc tatgatcatg gccctcaagc ggtattcaga
     6721 cggaatccgg ggccgggctc aaaagggatc cttcgaggtt gagatcctcg atctcaccgt
     6781 gcgctcgaac ctcgcggaat acaccttcgc cgcgtcggag aacatcatca aaaagcagga
     6841 aaagctcatt cacgacgtgt ctcatcaaag cgataaattc ctgaccttca ccaggggcat
     6901 atatgaagtc acgttgatca tcagcgaaga gttcagaaaa aacgtcgacg aaatcttcaa
     6961 aggcgaacgg aagatttcct ctttttcgaa cctttcggcg gtcattctca ggcttcaccc
     7021 gaaaaccgtc tcgacccccg gcgtgcatta ttccattctc aaacagctcg cctggtcgaa
     7081 tatcaacgtc atcgaggtcg tttccacata cacggaattc acggtcatca tcgacaatgc
     7141 ccggatcgac acggcttttt ccgttctgaa gaacctgatg atgagataaa aaggaaatcc
     7201 gggacgtatc cggaccctcg cgggccggat cgccgcccgg ctctccacac ccgccgccgg
     7261 ccggaaggac gacgcgcgcg aggtccgccg gaaaggtctt gacggcgaac gccgcggagc
     7321 ggacccgaat ttccaggacg atctccggcc tccccggcag ggaaaaaacc gcgggagcgg
     7381 ttcagaagcg gtacccggcc tgcttggcga ctttccggta atcctgcccg gagatctcga
     7441 ccaccttgca catctcgtag atccgcgagc ggaggcggac gccgatccgg tcgacgagcg
     7501 tgtcctcggg cttgcgggtc gcgccgaaac cggccgtttt cttgaacatc ggggcccggt
     7561 cgtcggccgt ctcggatgtg tcggggtagt tcgaggtgaa gatcgtcagg cgccgctcgt
     7621 tgtaacggcg gttgatgatg tagaagatcg tttcctcgac ccaggtcgac gaccgctggg
     7681 cccccagctc gtccaggacc aggacgtcgc tttcgaagat cggccggagg acgtcggact
     7741 cgctgacgtc ggaatcgggg gcgaacgtgg cctggatgtc gcggatcagg tcccggaaat
     7801 cgtagaagac gccggccgcc cttttcttcg tcaccagctc ggccaagatg gccgtggcca
     7861 agtgggtttt ccccacgccg caggggccga tgaacagcag cccgatgtct tgggccggga
     7921 aattggagat gaatttcctc gagatccgga gggcgtcctt gtgggagtca tcatggacct
     7981 cgaaattcgc cagggtgcag tcgcggtagc gggtggggat gcgggtccgg aggaggacgg
     8041 cgctttcggt tttcgcctcg tggcaggaac agcggcgggc cgcggggccc gctttcgttt
     8101 cgacctgaat ccagcccgtg tcaaagcagc gcgggcattc ctcgcgtccc ttgttcttca
     8161 cctcattcgt ccccgttcag ttcaagtatc cgcgaagctc ctggagcttg tgggaccggc
     8221 tcagcttggt catggccgtc tgttcgatct ggcggatccg ctcgcgggtc aggtggaggc
     8281 ggtcgccgat ctcctggagc gtcagcggcc ggtcgtcgtc caagccgaag cgtaatttta
     8341 acacagtcgc ttccttctca tccagctcgc ttaggatctc ccggacctgg ttcaagatgg
     8401 aagccttgat gatctggtat tcgaccgagg gggagatcgg atcgccgatc ttctcctcca
     8461 gcccctggcc gtcctcgtcg aaccggtcgt tgagcgagat ttccttgttt tccaacaggg
     8521 ccaggtcctc gatctcggag accgtcatct tcatcgccgc cgcgatctcc tcccggttcg
     8581 gctcccgccc cagctcggcc gacaactggg cgactttccg cttcatcagg agcatctggt
     8641 cggagatttt ctgggggaca tgggtgacgc gggccgaggc ggtgagcgaa tgaatcaccg
     8701 cctggcggat ccaccagacg gcgtaggaga tgaacttgac gttgcggccg ggatcgaacc
     8761 tcttggccgc ctcgatcaag ccgagattgc cctcgttgat gaggtcgagc atgtccaagc
     8821 ccattccctt gtattttttg acgtaggaga cgacaaaccg caggttgccc tcgatcatcc
     8881 gctcgagggc cttggcatcc ccggcatgga tgcgctcggc cagctctctt tcctgctcct
     8941 cggtcaagcg cggaaaacgg ccgatctgct ccaagtagcg gcggaggttc cgcgattccg
     9001 tgaaatcctt gtaggacgtc attcggattt gtcgactttc acgatctggg acgacgagac
     9061 gtggacgagg ctttggcggc gggcctcgaa ctcctcctcc cggccggaga agaactcccg
     9121 ctggaaggaa tccatgaagg cttggacctc ggcttcgatc cgctcgattt tcgcccgcat
     9181 gttcaagatg acttccaccc ccgcgaggtt gacgcccagg tcgcggacaa gattgaggat
     9241 gatctccagg cgtttcaaat cctcgtcgga atagagccgg gtgttgccgc acgagcggga
     9301 cggtttgagc agcccctctc gctcgtagag gcggagcgtc tgcggatgga tatggaaccg
     9361 gtcggccacg ctgctgatat ggtagtagct cgatcccggg cacccagcct cgccggcctt
     9421 gggccgcggc cgccgcggcc cggcggtttc ggctttccgg tccgccggcc ggggcttcgt
     9481 tttattcatt gtgtttcttt tcatcgtgga acctcttcat tcattccggc ccgcggactg
     9541 cccccgtaga ggggttcgag cttcttcatc aggtcccgga cgcgctcgtc gccgaagggc
     9601 ggcgggacga tcatgacctc gacgaattca tcgccgcgga tccgggcccc gggctgcggc
     9661 gctcccttgc ccttcaatcg gaagcgctgt ccggatttcg tccccggcgg gatgcggatg
     9721 acggccgggc cgtccagcgt cgggacgtcg attttcgccc ccagggtcgc ctcgggaacc
     9781 gtgatcggga ccttgacgtg gaggctcatg ccctcccgcc ggaagagcgg atggggcagg
     9841 gtctcgatct ggatgtaaag atcccccgga ggaccgccgc ccaggccggc gtggcccttt
     9901 cgggggatgc ggaccttgct tcccgtgtcc accccggccg ggatgcggac gttgatcgtt
     9961 tcggtgatct cccgcgtccc ctgtccccgg cagtcgccgc aatcacgccc ggccacggtc
    10021 ccgcggcctc cgcaatccgg gcagacgccg gaaaacttca tgtagccgcg ctgctgaaag
    10081 gccttgccgg atccccggca cgtcgggcag gtcttggcct cgccgctttt ggagcccgat
    10141 ccgccgcagg ccggacaggc cgcccgccgg ttcaggcgga tcttggtctg gacgccgcga
    10201 acggcgtcct cgaacccgac tttcatcgtg tagagaaggt cttcacccct ctccgggccg
    10261 gccgcggccg ccgcccgggc ggccgccccg ccgaagatgt tctgaaagag gtcgcggaac
    10321 gatcccccgc cgaaatccga aaagtcgaat ccctcgaagc cgcccgaggc gtagtcgccg
    10381 ccgccgggcc cgccgtcggc cggagggcgc tcgccggcga acccgaattg atcgtaccgg
    10441 gctttcttct tgggatcgct gagaacctcg taggcggccg aaatctcctt gaatttggcc
    10501 tcggccgcct tgtcgttggg attgagatcg ggatggtgct tgcgggccag acggcggtag
    10561 gctttcttga tttccgcgtc cgaagccgac cgtccgactc cgaggatctc ataataatcc
    10621 ctggccatgc gctcccctct ttcctttccc ccgtctttac ccggggaaaa atcgccggag
    10681 gagcggaggg ccgctcctcc ggacattcaa tcccatcccg gccgggccgg gcgtcagttt
    10741 gattttccgt cgtcgacgac ttcggcgtcg atgacgtccc cctcggaccc gccgtttccg
    10801 ccggacgggc cggacgaagc ctgctcggga ccgtcggagg cggcccccgc tccgggctgc
    10861 gggccggcgc tccggtagag aacctcggtc atcttgtggg aggcccggtt gaggctttcc
    10921 agggctttct tgatggcctc ggggctgtcg gattcgacgg ccttcttggt cgcctcgatc
    10981 tcggaccgga cggcctcggc ttcggccgcc gggatcttgt cggcgttttc ccgaaggatt
    11041 ttttccaggc tgtagatcat ctggtcggcc tggttgcggg cgtcgatcgt ctcccggcgc
    11101 tttttatcct cggtcgaatg agcctcggcg tcggagacca tccgcttgat ttccttctcg
    11161 tcgagcccgg aatgcccggt gatggtgatc tgctgctgct tgcccgtccc catgtccttg
    11221 gccgagacat ggagaatgcc gttggcgtcg atgtcgaagg tgacttcgat ttttggaatg
    11281 ccgcggggcg ccggcgggat gccgtccaag tggaaacggc ccagggtgcg gttgtccgag
    11341 gccatctccc gctcgccctg gaggatgtgg atctcgacgc tcggctggct gtcggaggcc
    11401 gtggaaaaaa cctcggtttt cttcgtcggg atcgtcgtgt tccggctgat catccgggtg
    11461 aaaacgcctc cgagcgtttc gatgcccagg gtcagcggag tcacgtcgag aagaaggacg
    11521 tccttgacat cgcccgaaag gacggcgccc tggatggccg cgccgacgga gacgacttcg
    11581 tcggggttga cgcctttgtg gggctcgcgg ccgaagacct gctggaccag cgcctggatg
    11641 cggggcatcc gggtctggcc gccgacgagg atgacctcgt ggatgtcctc gggcttcagg
    11701 ccggcgtccg acagggcctg cttgcagggg gccacggacc gttggatcaa atcctcaatg
    11761 agggcctcga gtttcgcccg ggtcattttc atcaaaaggt gcttggggcc cgaggcgtcg
    11821 gccgtgacga acggcaggtt gatctcggtc tccatcgtcg tcgaaagctc gatcttcgcc
    11881 ttttccgcgg cttccttgag acgctggatg gccatcttgt ccttggccag gtcgatgccg
    11941 gtttccttct tgaattccgt cgtgatccaa tcctggatcc gttgatcgat gtcgtcgccg
    12001 ccgaggtggg tgtcgccgtt ggtcgatttg acctcgacga cgccttcccc cacttcaagg
    12061 atggaaatat cgaacgttcc gccgccgaag tcgtagacgg cgatcgtctg gtccttcttc
    12121 ttttccatgc cataggccag ggccgcggcc gtgggctcgt tgatgatccg gaccacatcc
    12181 aggccggcga tttggccggc gtccttggtc gccttccgct ggctgtcgtt gaaatacgcc
    12241 ggaacggtga tgaccgcctt ctcgactttt tccccgagat aatcctcggc cgcctttttc
    12301 agcttctgaa gaaccatcgc cgagatctcg ggcggcgagt actgcttgtc gaagacagcg
    12361 atccggacgt cgccgttggg cgcttccgtg atcctgaacg gcacctgctt gatttcgttc
    12421 gggacctcgg cgtaccggcg gcccatgaac cgcttgatcg agtagaccgt gttttcggga
    12481 ttggtgacgc gctggcgctt ggcgacctgc ccgaccagtc gctcgccctt ggccgtgaat
    12541 ccgacgacgg acggagtcgt ccggccgcct tcctcgttgg agatgaccgt cgcttggtcg
    12601 ccctccatga tggccacaac cgagttcgtc gttcctagat cgatgccgat aatcttagcc
    12661 atatatgata ctccttagca ttaagtcgat tgttaatata aaactttagc gtatcgttgt
    12721 caagtatttt attgcgatat attttattta tattcaatat aatacaaata ttttttacat
    12781 ttttattttt cgttcccaat taatccggat taaatcgctt tccctttata cggagatcgt
    12841 ttccgtcccc ccgccggaaa ttattccggc cagtaaagga tgcacttctg gccttcgaaa
    12901 aaagaccagt aaatgttttt ggccgcgctt tcctcgagaa agatgatctc gatttccgtg
    12961 aacggccgtc gtccgattcc ccggacgatc gttttcaccg accggccgat cccccgggaa
    13021 aacaaacgga ccgccccgtc gagaatccgg gcgaacccgc ttttccttag acggacgttc
    13081 atccggatgt cgccgggaag ctcaagcgtg aaattgaccg gcatcttcac cagttccagg
    13141 atgtcgagat cgtcgttttt ggccgtcggc ttcttgtccg tttcgtcctc ggccttgccc
    13201 ggcgtgacgt ccttcctctt gggcgcgttg taggcggttt ttttcaacag cgggcacgtc
    13261 atgactccgc cgtgccgccc ccaggttttc accttgaaga tcggccattc cttgagaacg
    13321 aggcccctgg ccttcaagga gacctttccg gcctcgaaat cgaaaataaa atacgtcgac
    13381 ggcgtccggg cgaggccgta ttcctgctcg acgcgggccg ctttccgcgg ggacggggcg
    13441 gccgcttcct gtccggccgc gccggcaacc agaagggcgg ttcccgcgac aagacctaaa
    13501 atccgtcggg ttttcatgtc agtagatgta gactttcgcg cccatgggca cgctttgata
    13561 gacttttttc agatcatcgt cccccacccg gacgcagccg tgggtcacgt tccggccgag
    13621 catccgggtg tacagcgttc cgtggatgaa ataaccgctg ccgatgccca gggcgtaatc
    13681 gcccagggtg tcgttgtcga tccggtcact gaaatttttc ggcagatcct ccccttcctc
    13741 gtaaaaggcc caatccggct tgatccaagt cgggttgacg agcttggagg ataccgtaaa
    13801 ttccccgcgg ggcgtgtcga aaacccactt tcgctcgccg ctgggatcct ggaggatgtc
    13861 gcccgatccg caggacacga tcgccagcag aagctctttg ccgtcccgga tcacccggag
    13921 gcggttttcg gccgtgtcga tcatgatata agtcccccgg ggcagcaggc ttttcatcct
    13981 cgattcgagc tgccgcgtct tagccgccag ggttttggcc tcgacgggcg gcgggatttc
    14041 ggcccgctcc tcgctccggg cccaggaacg ggcgattatg ttcgctgcga ggacgatcag
    14101 cccggccgcg aggatgaatc ccgcggccgc gatcagaaga atttttctga ttttagcgga
    14161 catttttctt gaatttccgg atttcatcaa ggatcgtgtt ttcgacgtcg gtcgccccga
    14221 cgatcgtcac cggcgtgccg acgaccgacc aggcgtagat gtcgtccata tccttgtctt
    14281 ccaacccaac acagcccttg gtaaggctgt ctttcccccc gccgtggatc tcgatggccc
    14341 ctccgatccc cgcatggctc ggaatctggc cccggcgctt ggcctcggcg aagaaccgct
    14401 tgtcgtcctc gttgggataa tcgattagaa gggctttata aaacttggag gccgggattt
    14461 tcttaacgac tttgtaccgc ccctccggag tcgcctcgtc cccggcccgg cgtttgtccg
    14521 aaaggccgta tttccccaaa ccgatgtcat accgggcccg gaccttgccc ttttcataga
    14581 cggtcagccg gcgctcgacc ttattgacga ggatcgccac gccgtcgccg tcctgggatt
    14641 ccttcacggc ctcctccgcc cagcgcctcc acttggccag ctggccggaa tccaggtagc
    14701 gctcaaggag ccggacggcc gcctcctcgg ctcgttccag aaaaacttcg ccggccgcgg
    14761 ccgcggccga agccggttca tatttttcct tggagaccag catccgggcc tcgaccagct
    14821 tgatctccgc ctgggaggcg tttttccgga tgtcgtcgtt ttcgttgaaa tattgggtga
    14881 tgcgatggag gctttcgatc cgggcggcca gcgtttcggc cgataaggcg aaggtcttgg
    14941 cccgggcggc cttgtcgtcg tcgatccgac ggagcagcgc ctctccctcg gcgacgagct
    15001 tttgagatcg gcctcgatcc gggaatagtc ccggaaccag ccgaaccggg cttcctccgc
    15061 ctcgatccgt ccgccggcgc tcttcaagcc
//
LOCUS       DAOOXH010000059        14518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_4195_length_14518_cov_7.015211, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000059 DAOOXH010000000
VERSION     DAOOXH010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 14518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14518
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4195_length_14518_cov_7.015211"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..1519
                     /locus_tag="PKV64_07765"
     CDS             5..1519
                     /locus_tag="PKV64_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPN17013.1"
                     /translation="MKTSSSALQGIEAYVVEVEVDIGPGLPDFIIVGLPDASVRESKE
                     RVKGALRNCGYDFKPHKVTINLAPADRRKEGPSFDLAIALAHLVFLDLVPAEPLRDYC
                     FLGELALDGRIKPVKGVLSAALLARERKFKGLVVPKDNEKEAALLEGLDVFGVSDLSQ
                     VVSFLHDPGRFRPARFALSELIEPPPPGPDFREVKGQHHVKRALEVAAAGGHNVLLIG
                     PPGSGKTMIARRLPSILPDMTFEEIIQTTQVYSAAGYLKGKGAVGLRPFRAPHHTVSD
                     AGLIGGGLVPRPGEVSLAHLGVLFLDELPEFKRKVLEDLRQPLEDGTVTVVRSGHRAV
                     FPAAFMFVAAMNPCEDVFKGLSGGSGECTESQRSKYYAKISGPLLDRIDIQVEVPALK
                     FADFKSKAEAESSTAIKARVTAARERQRARFSGKGIFANVQMGVREIREFCRIGPEAE
                     KLLEEAVSRLGFSARAYDRSLKVARTIADLAGAEEISAAHLAEAIQYRAMDRYY"
     gene            1635..2345
                     /locus_tag="PKV64_07770"
     CDS             1635..2345
                     /locus_tag="PKV64_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPN17014.1"
                     /translation="MQLTVKDVTKLLNISEKTIYRWLKQGSLPAYQVGNQYRFNKAEL
                     LEWATSRRLGVSVDLFRENSEEAGQSKGLAEALSAGGIHYRIEGGDKETVLGTVVDLM
                     KLPREVDRNFLLQVLLAREAIASTAVGDGIAIPHVRNPIVLHVTEPMVSLCFLEKAID
                     FGSLDGRPVNCLFTLVSPTVKSHLHLLSRIAFALRDGDFRKLILKQGGRNEIFAEIAR
                     VEAGLDQSKISSKADRSE"
     gene            2346..4322
                     /locus_tag="PKV64_07775"
     CDS             2346..4322
                     /locus_tag="PKV64_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPN17015.1"
                     /translation="MALFLLGLAVIFSGGLASLLWGRKPGAADGLGAAGAVAGGVIAA
                     VPAFRILLGAPAASLQWNWNIPYGSFYIQIDSLSAFFLIPIIGLSVIAAVYGSDYLRA
                     YRDRKNLGVAWFFYNLLLIGMMLVVVARNGVLFLIAWETMSLASFFLVTFEYEKPMVR
                     RAGWIYLIAMHIGTAFLIVFFLLLGRHAGSLNFDAISGVPSSLASALFLLAIVGFGTK
                     AGFMPLHVWLPYAHPAAPSHVSAVMSGVMIKTGIYGLVRTLTILGPPQEWWCWTLIAI
                     GAVSGIAGVLFALAQHDLKRLLAYHSVENIGIITLGLGLGLLGVNMNAPTLAVLGFAG
                     GLFHVVNHAVFKGLLFLGAGSVIKSCGTGQIDALGGIIKRMPWTAFAFLTGSIAISGL
                     PPLNGFASEFLIYLGVFRGDIDAGFKAAIPELAIIGGLALIGGLALACFTKAFGIIFL
                     GHPRSEAAERAREAGWGMRAATTALAVLCVLLGLLSPLVIQSVKGPISLISGFDDELA
                     RVVLGYATRILIGFVSVSAVLIAVTAFFAVLRRRLLRGRSVRRAPTWDCGYARPTPKM
                     QYTASSFAQPLTDLFKIFLRSKKDVSLPEGLFPGRSHFHTDTKDVSEEYLHEPAFEWI
                     TSFLGKFKWLQHGRLQIYILYIALTLLALLIWKL"
     gene            4327..5235
                     /locus_tag="PKV64_07780"
     CDS             4327..5235
                     /locus_tag="PKV64_07780"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPN17016.1"
                     /translation="MTILLKLLNTAAGLAFAPLLIGVINRTKAWCAGRTGPPLLQPYH
                     DLRKLFRKGVVYSESSSWILRAGPVVGLSAVVTAATIIPFGGLPVLLSFNGDLILFAY
                     LFGLARFFTILMALDSGSSFEGMGASREAAFSALAEPVLLLTLAVIAKYTGQISFSKI
                     FPAIDFGYWPKAGPAFVLIGLALLIVFLAENARIPVDDPNTHLELTMIHEAMVLDHSG
                     PDLGMIHYAAAVKMWLLGALLTQIIVPVRGGGRLADVAAALLAMMLLAAVVGLIESSM
                     ARLRLQRVPQLLVGAAALSILAAILV"
     gene            5245..5889
                     /locus_tag="PKV64_07785"
     CDS             5245..5889
                     /locus_tag="PKV64_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17017.1"
                     /translation="MTNSVDILMITLVLANLALLGISRLNTCIRIVAAQGIALGLLPL
                     ALNGWRPGWHLILFSLLLIGLKGIVFPAILVKTLRDLKTSREIEPFIGYGASIAAGAV
                     ALVVCMWLGSRLPRPVDVSSSLLVPVALFTIMSGFLLIISRKKALTQVLGYLVLENGI
                     FAFGVALVEEQPFLVEIGILLDVFAAVFIMGIAIFHINREFDHIDTDKLSNLRT"
     gene            5894..7369
                     /locus_tag="PKV64_07790"
     CDS             5894..7369
                     /locus_tag="PKV64_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009109226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPN17018.1"
                     /translation="MIWSLLLIPAAAAAASLIIRGSLARRVVLVAAAAGHTAVTLAAW
                     TARPKPLLNGWLLLDDPGLLFLTITSLLFLACSIYAFGYLAGDPGGKQQDFVEGLLFT
                     NAPQAIFCACLLAFLATMTLVTLCQHFGLLWVAMEATTLASAPLIYYHRHRRSLEATW
                     KYLLICSVGIALALLGNFFLVVAASNPGTAGKSIVLSVLIENGQALDVPWLKAAFLFF
                     LVGYGTKLGLAPLHTWLPDAHSESPSVVSALLSGALLNCAFLGILRSYQVCAAAGEAD
                     FCRGLFVLFGLVSIGWAAVFMLGQADFKRLLAYSSVEHVGIMALGVGIGADAVFGSMF
                     HAVNHSLTKAMLFLAAGNILAVYKTKSVGKVSGVLTVMPATGMLWTAGFLAVTGIPPF
                     GIFLSKLTIAKAMIDQGKTGLAAAFLALLALIFAAMSSVVLNMVHGRKSAEAGAGAKP
                     ASLLELLPPAALGAATLLLGLYIPPAVSRLLHGVAAAFGGR"
     gene            7369..8892
                     /locus_tag="PKV64_07795"
     CDS             7369..8892
                     /locus_tag="PKV64_07795"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPN17019.1"
                     /translation="MNTGPSVAIYNGQSVGTGDIPVAGVEEFRTFVIEAAAAGLGLAS
                     LFAVPSRPGRFRLYAVLAADETGRLYVSSTEVPDEYPSITPDCPQAHLFEREVAEQWA
                     IVPRGHPWLKPVRFHRSSVPGRDAFGRSEGRPILPSVMDFFQVEGEQIHEVAVGPVHA
                     GIIEPGHFRFQCHGEEVLHLEISLGYQHRGIERALIGGPGVRTLHFIENVAGDTAVGH
                     ATAYGQMLESLSGVPAAPRAQSLRGIALELERLANHTGDLGALAGDVGYLPTSSFCGR
                     IRGDFLNMTASLCGNRFGRGVVAPGGVHDDLTEEDRGALSEKLARAGKDVSVAVKLLW
                     STPSVLARFEETGVLTADQCRTLGLAGVPARACGLETDIRFNFPSGIYRFSQMPVSTW
                     PSGDVFARAYVRWLEIQRSIAFILEQLRTCPPGPVRVEVKAPAADRFCVSLVEGWRGR
                     ICHVGITDSGGRFSRYKIVDPSFLNWTGLALAMRNQPVSDFPLCNKSFNLSYCGHDL"
     gene            8917..9675
                     /gene="nuoB"
                     /locus_tag="PKV64_07800"
     CDS             8917..9675
                     /gene="nuoB"
                     /locus_tag="PKV64_07800"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HPN17020.1"
                     /translation="MKALLARLQQKHRTISFPKGEPRLPDRYRGLPILNEAGCETGCR
                     ACLEECPTGALAKTDGRLSLDLGRCLFCSRCERACPAGAVRFGRDYRQAARSRNDLVV
                     GGPEIRRAAALEKKLKNLLGRSLKLRQVSAGGCNACEADTNVLTTIVYDIGRFGIQFE
                     ASPRHADGLLVTGPVTENMRSALEKTYAAVPDPKIVIACGACAVSGGVFADLPDVQNG
                     VEKTIPVDLFIPGCPPHPLTILDGLLRLAGRING"
     gene            9795..11465
                     /locus_tag="PKV64_07805"
     CDS             9795..11465
                     /locus_tag="PKV64_07805"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPN17021.1"
                     /translation="MAWKNRRPRGGAAAVAAVAVLLVFSSAAPPEDTVLFRIERADLD
                     RLSADAAAGLFVVQELQTCLLASGPAALPGELAEAGVPAVVLDGKAEGKIYFLVRTPR
                     PEEGDILVGLGTAVPLEERVWLFSPASGDPREIIPPAFRLKRLDLDRSVVWSRGAPAG
                     ETAAGAVEAIVPDPRIFEWVARVSKSKLTETIQSLQNFQTRYASTAACENAGTFLYNR
                     FVQLGLACEYETFTFSWAQRQSRNIIATLPGKAVPNRVVIVCAHYDSISDEPETLAPG
                     ADDNASGTAAVLEIARVLAAAPLDYTVKFICFSAEEWGLYGSSFYAQRAKSRGERIVG
                     VINMDMISYTDSDDPLLEVYVNPASRWLADQYLEAAETYAPLAASKITDSSATWSDHS
                     PFWDQGFAALCAIEESAGRNPYYHETTDTLDKLNMNYCTSVTRASLASAADLARPHVV
                     RPAPPTGLQARSEIISSLFAAVKSVSLAWDAGPASVAGYNVYRTTTSGGGHVKINSRL
                     LSEPRFSETMLDPATTYFYEVTAVDGQGLESDGSDEVRDDQNNDALPN"
     gene            11434..12081
                     /locus_tag="PKV64_07810"
     CDS             11434..12081
                     /locus_tag="PKV64_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17022.1"
                     /translation="MIKTMTRFRIKAGLRAAPVFWWLILGMGPAGLRASTAAGRPRTA
                     VSLEAGMFRAGQAAFRTLYGNGISALSAHVRVRLIDGLWAAAGWRTVKAGGATAIAGP
                     AFNEEEYALEFEMTSIRLGLRYAFDLGRIAVFAGAGGSADRYEERWVDVPGLGGSGKK
                     AGWYAEAGGEYAVFRFLSLSLRGEYTSVPTARGSSLEPDVNLGGLEAAAGLVFRF"
     gene            12185..12547
                     /locus_tag="PKV64_07815"
     CDS             12185..12547
                     /locus_tag="PKV64_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17023.1"
                     /translation="MKIRKWAVLYSLIVGVSMIGMWTMFFASGGIPELKTKPVEINLH
                     LAAELSTAVLLLIGGWGLIKRRPWGTQAHSLSMGMLLYTLIASPGYYLQKGELPFVFI
                     GLSFFKKEDFLSEPRRRD"
     gene            12905..14269
                     /locus_tag="PKV64_07820"
     CDS             12905..14269
                     /locus_tag="PKV64_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPN17024.1"
                     /translation="MKRFKTARRPAVRRLSPARLFILSFLLVILIGALLLLLPAASVR
                     EPLSLMDALFTSASAVCVTGLTVVDIGRDLSLFGQVVTLLLFQIGGLGIITFSLVLFG
                     LMGRSLSFRGRELLQSTFLHTPRRDFFRILRFVVSGTFVIEGAGTLVLFLRFLSDFPP
                     GRALYQAVYHAVSAFNNCGYSLFADNLARYRDDPVVNLAIVSLIVLGGLGFIVMYEVI
                     DRWRGRQKRLSLHARVVLLTTGALITGGALLFYLFEAGNVLVGVRGPGRVLASLFQSV
                     TPRTCGFNTVDIAALANPTILLMMILMFIGASPGSTGGGIKTSSFALLGLMIINRIRG
                     RDGVTIFRRTVPQEILGRTIAIVLASAVSVLVIASVLLLAAGPGAGPDSRHLFVEYMF
                     ETLSAFGTVGLSMNVTAALNEIQKLAIVLMMFMGRVGPLTLAFAWSTFRRGLVYAEEG
                     VMVG"
     gene            14278..>14518
                     /locus_tag="PKV64_07825"
     CDS             14278..>14518
                     /locus_tag="PKV64_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPN17025.1"
                     /translation="MKRCVVIGLGIFGQNIVREISDKGFEVIAVDKNKEAVQRVRDLA
                     GKAIVADGTDKDLMDQIGIHDDRVVLMDADLVHEVL"
BASE COUNT         2285 a         4761 c         4519 g         2953 t
ORIGIN      
        1 gctcatcaaa acgtccagct ccgccctcca ggggatcgag gcctatgtcg tcgaggtcga
       61 ggtcgacatc ggccccggtt tgcccgattt catcatcgtc ggcctgcccg acgcctccgt
      121 ccgggaaagc aaggagcggg tcaagggcgc cctccgcaac tgcggctacg atttcaagcc
      181 gcacaaggtc acgatcaacc ttgccccggc cgaccgccgg aaggaggggc cgtctttcga
      241 cctggccatc gccctggctc atctcgtctt tttggacctc gtcccggccg agccgcttcg
      301 cgattactgt ttcttgggcg agctggcctt ggacgggcgg atcaagccgg tcaagggcgt
      361 gctgtcggcc gccttgttgg cccgggagag aaaattcaag ggcctcgtcg ttcccaagga
      421 caacgagaag gaggccgccc ttctcgaagg actcgacgtc ttcggcgtgt ccgacctgtc
      481 tcaagtcgtt tccttccttc atgatcccgg ccggttccgg cccgcccgct tcgccctctc
      541 cgagctgatc gagccgccgc cgccgggacc ggatttccgg gaggtcaagg gccagcacca
      601 tgtcaagcgg gccctggagg tggccgcggc cggcgggcac aacgtcctcc ttatcgggcc
      661 gcccggctcg ggcaagacga tgatcgcccg ccgcctgccc tcgatcctcc cggacatgac
      721 gtttgaggag atcatccaaa ccacccaggt ctacagcgcg gccgggtatt taaaaggaaa
      781 aggggccgtc ggcctcagac ccttccgggc cccgcaccac accgtttccg acgccggact
      841 gatcggcgga gggctcgtgc cccggccggg ggaggtcagc ctggcccact tgggcgtgct
      901 gtttctcgac gagctgcccg aattcaagcg caaggtcctc gaggatctcc gccagccgct
      961 ggaggacggg acggtgaccg tcgtccggtc cggccaccgg gcggtcttcc cggccgcctt
     1021 catgttcgtc gcggcgatga atccctgtga ggacgtattc aaggggttga gcggagggag
     1081 cggggaatgc acggaatccc agcggtcgaa atattacgcg aagatctcgg ggccgctgct
     1141 cgaccggatc gatatccaag tggaagtgcc ggccttgaaa ttcgccgatt tcaagtcgaa
     1201 agcggaggcg gagtcctcaa ccgcgatcaa ggcccgggtg acggccgccc gggagcggca
     1261 gagggcgcgg ttttcgggca aggggatatt cgccaatgtc cagatggggg tccgggagat
     1321 tcgggagttc tgccggatcg ggccggaggc tgaaaagctc ctggaggaag cggtctcccg
     1381 gctcggcttc tcggcccggg cttatgaccg gtcgctcaag gtcgcccgga cgatcgccga
     1441 tctggcggga gccgaggaga tctccgccgc tcatctggcc gaggccattc aataccgggc
     1501 gatggaccgg tactattgaa gaaaagccgg acgtattatc aaacgccgcc ttgacaatat
     1561 atgacttgac ggatagagtg tcctttaatg tccaataaaa cggctcagcc gttttattcc
     1621 tgtagcaaaa agaaatgcag cttaccgtaa aagacgtcac gaagcttcta aatataagcg
     1681 aaaaaactat ttacaggtgg cttaaacaag gcagcctgcc cgcttaccag gtcggaaacc
     1741 aataccgttt caacaaggct gagctcctcg agtgggcgac gtcccgccgg ttgggcgttt
     1801 ccgtggattt gttccgcgaa aacagcgagg aggccggcca aagcaaaggc ttggcggagg
     1861 ctctgagcgc gggcggcatt cattaccgca tcgaaggcgg cgacaaggaa accgtcctcg
     1921 gaaccgtggt cgatctcatg aagctgccgc gggaagtgga ccggaatttt ctcctgcagg
     1981 tgctcttggc ccgcgaggcg atcgcctcca ccgccgtggg cgacggcatc gccattccgc
     2041 acgtccgcaa cccgatcgtc cttcatgtca ccgaacccat ggtttccctt tgcttcctcg
     2101 agaaggccat cgacttcggt tcgctcgacg gccggccggt caactgcctt ttcaccctcg
     2161 tcagccccac ggtgaaatcg catctccatc tgctgtcccg gatcgccttc gccctccggg
     2221 acggcgattt tagaaaactc atcctgaaac aaggcggacg aaacgagatt ttcgccgaaa
     2281 tcgcccgagt cgaggccggg ctcgatcaat ccaaaatttc ctcgaaagcg gatcggagcg
     2341 agtaggtggc cctcttcctg ctcggtttgg ccgtcatttt ctcaggcggg ctggcctcgc
     2401 tgctgtgggg gaggaagccg ggcgcggcgg acgggctcgg ggccgccggg gccgtcgccg
     2461 ggggagtcat cgccgccgtc cccgccttcc ggattctgct cggcgccccc gccgcctcgc
     2521 tccagtggaa ttggaatatc ccctacggct cgttttacat ccagatcgat tccttgtcgg
     2581 cctttttcct gattcccatc atcgggctgt cggtgatcgc cgctgtttat ggatccgatt
     2641 acctccgggc ttaccgggac cggaaaaacc tcggggtcgc ctggtttttc tacaatcttc
     2701 ttttgatcgg catgatgctg gtcgtcgtcg cccgcaacgg cgtgctgttt ctgatcgcct
     2761 gggaaacgat gtcgctggcc tcgtttttcc tggtcacctt cgaatatgaa aagcccatgg
     2821 tccgccgcgc cggctggatc tacttgatcg cgatgcacat cggcaccgcc tttctcatcg
     2881 tcttcttcct cctgctcggc cgccacgccg gatcgctgaa tttcgacgcg atctccgggg
     2941 tcccgtcttc gctggcgagc gccttgttcc tgctcgcgat cgtcggcttc ggaaccaagg
     3001 ccggcttcat gcccctgcac gtgtggctgc cgtacgctca cccggccgcc cccagccatg
     3061 tttccgcggt catgagcggc gtcatgatca aaaccggcat ctacggactg gtccggacgt
     3121 tgacgatcct ggggccgccc caggaatggt ggtgctggac attgatcgcg atcggggccg
     3181 tttcgggcat cgccggggtt ctcttcgcct tggcccagca cgatttgaag cggctcctgg
     3241 cctatcacag cgtggagaac atcggcatca tcaccctcgg gctgggcctc gggctgctgg
     3301 gcgtcaacat gaacgcgccg acgctggctg ttctcggctt cgccggcgga ttgtttcacg
     3361 tcgtcaacca cgccgtgttc aaaggcctcc tcttcctggg cgcgggttcg gtcataaaga
     3421 gctgcgggac cgggcagatc gacgccctcg gcggaatcat caagcggatg ccctggacgg
     3481 cgttcgcctt cctgaccggc tcgatcgcga tttccggcct gcctcccctg aacgggttcg
     3541 ccagcgaatt cctgatttac ctcggcgtgt tccggggcga tatcgacgcc gggttcaagg
     3601 cggccatccc ggagctggcg atcatcggcg gcctggccct gatcgggggc ttggccttgg
     3661 cctgtttcac gaaggcgttc ggaatcatct ttctcggcca tccccgaagc gaggcggccg
     3721 aacgggcccg cgaggccggc tgggggatga gggcggcgac gacggctctc gcggtcctgt
     3781 gcgtgctgct cgggttgctc tctccgctgg tcatccaatc cgtgaaggga ccgatttccc
     3841 tgatttcggg attcgatgac gagctggccc gcgtcgtcct gggctacgcg acccggattc
     3901 tgatcggctt tgtctccgtt tccgccgtcc tgatcgccgt gacggcgttc ttcgccgtcc
     3961 tccgccggcg gctcctgcgc gggcgcagcg tgcggcgggc gccgacctgg gattgcggct
     4021 atgcccgccc cacgcctaaa atgcagtaca cggccagctc gttcgcccag cccctgaccg
     4081 acctgttcaa gattttcctg cgcagcaaga aggacgtttc cctcccggaa ggcctcttcc
     4141 cgggccggtc gcatttccac acggacacca aggatgtcag cgaggagtat ctccacgagc
     4201 cggctttcga atggatcacg tcgttcctcg gcaaattcaa atggcttcag cacggccggc
     4261 ttcaaatcta cattttgtac atcgccctga ccctgctggc ccttttgata tggaagcttt
     4321 aacgaaatga cgatcctatt gaaattgctc aacaccgcgg ccgggctggc cttcgccccg
     4381 ctgttgatcg gcgtgatcaa caggaccaag gcctggtgcg cgggccggac aggcccgccc
     4441 ctgctccagc cgtatcatga tctccggaag ctgttccgga agggcgtggt atacagcgag
     4501 tcctcttcct ggatcctccg ggccggcccg gtcgtgggat tgtccgcggt cgtcacggcc
     4561 gcgacgatca ttcccttcgg cggcctgccc gttctcttgt cgttcaacgg cgatctcatc
     4621 ctgttcgcct acctgttcgg cctggcccgc ttcttcacga tcttgatggc cctcgacagc
     4681 ggctcgagct tcgaggggat gggcgccagc cgggaggcgg cgttctcggc cctcgccgag
     4741 cccgtcctgc tcctgacgct ggcggtcatc gccaaatata ccggccaaat ctctttttcc
     4801 aagatctttc ccgcgatcga cttcggctac tggccgaaag ccgggcccgc cttcgttctc
     4861 atcggcttgg ccctcctgat cgttttcctg gcggagaacg cccggatccc ggtcgacgac
     4921 ccgaacaccc acttggaatt gacgatgatc cacgaggcga tggtgctcga ccacagcggg
     4981 cccgacctcg gcatgatcca ctatgcggcg gccgtgaaaa tgtggctcct gggagcgctc
     5041 ctgacccaaa tcatcgttcc cgtgcgcggc ggcggtcggc tcgcggacgt cgccgcggcc
     5101 ctgctggcga tgatgcttct ggccgcggtc gtgggactca tcgaatcttc catggcccgc
     5161 ctgcggctcc agcgcgtgcc ccagcttctg gtcggcgcgg cggctctttc catcctggcg
     5221 gcgatattgg tttagggcgg catcatgacg aattccgtag acatcctcat gatcacgctg
     5281 gtgctggcga acctcgccct gctcggcatc agccggctga acacgtgcat ccgcatcgtc
     5341 gccgcccagg gaatcgccct ggggctgctg cctctcgccc tgaacgggtg gcggccggga
     5401 tggcatctca tcctcttttc cctgctgctg atcggcttga aggggatcgt ctttcccgcg
     5461 atcctggtca aaacgctgag ggatctgaag acgagccgtg aaatcgagcc gttcatcggc
     5521 tacggcgcct cgatcgccgc gggggcggtc gccctcgtcg tctgcatgtg gctcggctcg
     5581 cgcctgcccc ggccggtcga cgtctcctcg tccctgctcg ttcccgtcgc cctgttcacc
     5641 atcatgtcgg gctttctcct gatcatcagc cggaaaaagg cgctgaccca ggtgctcggc
     5701 tacctcgttc tcgaaaacgg catcttcgcc ttcggcgtgg ccctggtgga ggaacagccg
     5761 ttcctggtcg agatcggcat tcttctggac gtgttcgcgg ccgtgttcat catgggcatc
     5821 gccatctttc atatcaatcg agaattcgac cacatcgata cggacaagct gtccaacttg
     5881 aggacttgac atcatgattt ggagtctgct gctgatcccg gccgcggccg cggccgcctc
     5941 gctcatcatc cggggctccc tggcgaggcg cgtcgtcctc gtcgccgcgg cggccggcca
     6001 tacggcggtc accctggcgg cctggacggc ccgcccgaaa ccgctcctga acggctggct
     6061 ccttctggat gatcccggcc ttttgttcct gacgatcacc agcctccttt tcctggcctg
     6121 ctcgatctat gcattcggct acctggccgg ggatcccggc gggaaacagc aggatttcgt
     6181 ggaaggcctt cttttcacga acgcgcccca ggcgatcttc tgcgcctgcc tcctggcctt
     6241 cctcgcgacc atgaccctgg tcaccctctg ccagcatttc ggcttgttgt gggtggccat
     6301 ggaagccacg acgctcgcca gcgcgcccct gatctattat caccgccacc gccgctcgct
     6361 cgaggcgacc tggaaatacc tgctcatctg ctcggtcggc atcgccctgg ccctgctcgg
     6421 caactttttc ctggtcgtcg ccgcctcaaa tcccgggacg gccggcaaat ccatcgtctt
     6481 gtccgttctc atcgaaaacg gccaggcttt ggacgttccc tggctcaagg cggcctttct
     6541 gtttttcctg gtcgggtatg gaacaaagct ggggttggcg ccgcttcaca cctggctgcc
     6601 cgacgctcac agcgaatccc cctcggtcgt ttccgcgttg ctttcggggg cgctcctgaa
     6661 ctgcgctttt ctcgggatcc tgagaagcta ccaggtctgc gcggccgcgg gcgaggcgga
     6721 cttctgccgg ggattgttcg tgttgttcgg cctggtttcg atcggctggg ccgcggtctt
     6781 catgctgggc caggccgatt tcaagagatt gctggcgtat tcgagcgtcg agcatgtcgg
     6841 catcatggcc ctgggcgtcg gcatcggcgc cgacgccgtc ttcggatcca tgtttcatgc
     6901 cgtgaatcac tcgctcacca aggcgatgct gttcctggcc gccggcaata tcctcgccgt
     6961 ctacaagacc aagtcggtcg gaaaggtcag cggcgtcctg accgtcatgc ccgccaccgg
     7021 catgttatgg acggcgggct tcctggccgt caccggcatc cccccgttcg gcatcttcct
     7081 gagcaagctc acgatcgcca aggccatgat cgaccaggga aaaaccggcc tcgccgccgc
     7141 cttcctggcc ttgctggcct tgatcttcgc ggccatgtcg tccgttgttt taaacatggt
     7201 ccacggacgg aaaagcgccg aggccggggc cggcgccaag ccggcgtcgc tgctcgagct
     7261 cctgcctccc gcggccctcg gcgccgcgac cctgctgctc ggcttgtata tcccgcccgc
     7321 ggtcagccgc cttcttcacg gcgtcgccgc ggcgttcgga ggacgctgat gaacaccggg
     7381 ccctccgtcg cgatctataa cggccagtcc gtcggaacgg gggacatccc ggttgccggg
     7441 gtggaggaat tccggacgtt cgtcatcgag gccgcggcgg cgggcctggg cttggcctcc
     7501 ctgttcgccg ttccctcgcg tcccggccgg ttccgtctct atgcggtctt ggcggccgac
     7561 gagacgggcc ggctttatgt ttcctcgacc gaggtcccgg acgaatatcc ctcgatcact
     7621 ccggattgtc cccaggctca tttgttcgaa agggaagtcg cggaacaatg ggcgatcgtt
     7681 ccgcgcggcc atccgtggtt gaagccggtc cgcttccatc gttcctccgt ccccggccgg
     7741 gacgccttcg gacggagcga agggcggccg atccttccgt ccgtcatgga ctttttccaa
     7801 gtcgaaggcg agcaaatcca tgaggttgcc gtgggccccg ttcatgccgg catcatcgag
     7861 ccgggccatt ttcgtttcca gtgccacggc gaagaggtgc tgcatcttga aatctccctc
     7921 ggttatcagc atcggggcat cgagcgggcc ctgatcggag ggccgggcgt ccggacgctc
     7981 cattttatag agaatgtcgc cggcgacacg gccgtcggcc atgcgacggc ttacggtcag
     8041 atgttggagt ccctgtccgg cgtcccggcg gccccccggg cccaatcgct tcggggaatc
     8101 gccctcgagc tggagcggct cgccaatcac accggcgacc tgggcgcgct cgccggggac
     8161 gtcggttatt taccgacctc gtccttctgc ggccggatcc ggggcgattt tctcaacatg
     8221 accgcctccc tctgcggcaa tcgcttcggc cggggggtgg tcgccccggg cggcgtccat
     8281 gacgacttga cggaggaaga ccggggggcg ctctcggaaa agctcgcccg ggccgggaag
     8341 gacgtgagcg tcgccgtcaa gctgctttgg agcacgccct cggtcctggc ccgcttcgag
     8401 gagacgggcg ttctcaccgc cgatcaatgc cggaccctcg gcctggcggg cgtgccggcc
     8461 cgggcctgcg gcctggagac cgacatccgg ttcaatttcc cttccggcat ctatcgtttt
     8521 tcgcagatgc ccgtttccac ttggcccagc ggagacgttt tcgcccgggc ttatgtgcgc
     8581 tggctcgaaa tccaacggtc gatcgccttt atcctcgaac agctccggac atgcccgccg
     8641 gggccggtcc gggtcgaagt gaaggcgccg gcggccgacc gcttctgcgt cagcctcgtc
     8701 gagggctggc ggggccggat ctgccatgtc gggatcacgg attccggcgg gcgcttcagc
     8761 cgctacaaaa tcgtcgaccc gtcgtttctc aactggaccg gattggccct ggccatgaga
     8821 aaccagcccg tctccgattt tccgctctgc aacaagagct tcaatttatc ctattgcggc
     8881 catgacctgt aacctcaatc gaaggaagat caagcagtga aagcccttct cgccaggctt
     8941 cagcaaaaac accggacgat ctctttcccg aaaggggagc cccggctgcc ggaccggtac
     9001 cgcggccttc cgattttgaa cgaggccggc tgcgagaccg gctgccgggc gtgcctcgag
     9061 gagtgtccga cgggcgccct ggcgaaaacg gacggccggc tgagcctcga cctcgggcga
     9121 tgcctgttct gttcccgctg cgagcgggcc tgcccggcgg gggccgtccg gttcggccgg
     9181 gattaccggc aggccgcgcg gtcgaggaac gacctggtcg tcggcggacc ggagatcagg
     9241 cgggccgcgg ccctggaaaa gaaactcaag aacctcctgg gccgttcctt gaagctgcgg
     9301 caggtttcgg ccggcgggtg caacgcctgc gaggccgaca ccaacgtgct gaccaccatc
     9361 gtttatgaca tcggccggtt cgggattcaa ttcgaggcgt cgccgcgcca tgccgacggc
     9421 ctgctcgtca ccgggccggt gacggaaaac atgcggtcgg ccttggaaaa gacatacgcg
     9481 gccgtgccgg atccgaaaat cgtcatcgcc tgcggcgcct gcgccgtttc gggcggggtg
     9541 ttcgcggatc ttccggacgt tcagaacggg gtcgagaaaa cgattccggt ggacttgttc
     9601 attcccggct gcccgcccca tccgctgacg atcctcgacg gcttgctcag gctggcggga
     9661 cggatcaatg gttgacagcc cccgggcgct ctactaccct tgcggcaggg gattttcgga
     9721 tcagccggat tcctaaccgc ggcggagcgg aaaaggcttc ggctgaagcc ggaatcaaat
     9781 cccggggagg attcatggcc tggaaaaatc gtcgccctcg gggaggggcc gcggccgtcg
     9841 ccgcggtcgc cgtccttctc gttttttcct ccgccgcccc gcccgaagac accgtccttt
     9901 tccggattga gcgggccgat ttggaccgcc tgtcggccga tgcggccgcc ggtctttttg
     9961 tcgtccagga gcttcaaacc tgcctcctgg cctcaggccc cgccgctctc cccggggaac
    10021 tggccgaagc cggcgttccg gcggtcgtcc tcgacgggaa ggcggaaggg aaaatctatt
    10081 ttcttgtccg aacgccccgg cccgaggagg gggacattct cgtcgggctc gggacggcgg
    10141 ttcctctcga agagcgcgtc tggctctttt ccccggcctc cggggatccc cgcgagatta
    10201 tccctccggc tttccggctg aaacggctcg atctcgaccg gtccgtcgtc tggtcgcggg
    10261 gggcgcccgc cggggaaacc gcggccggag cggtcgaggc gatcgttccg gatccccgga
    10321 tcttcgaatg ggtcgcccgc gtttcgaagt ccaagttgac cgagaccatt cagagccttc
    10381 agaatttcca gacacgctat gcctcgaccg ccgcctgcga gaacgccggg acgtttcttt
    10441 ataaccgctt cgtccagctc ggcttggcct gcgagtacga gacgttcacc ttttcctggg
    10501 ctcagcgcca aagccggaac atcatcgcca ccctgcccgg aaaggccgtc ccgaacaggg
    10561 tcgtcatcgt ctgcgcccac tatgattcca tcagcgacga gccggaaacc ctcgcccccg
    10621 gggccgacga caacgccagc ggaacggcgg ccgtcctgga aatcgcccgg gtgctggccg
    10681 ccgcgccgct cgactacacc gtcaagttca tctgcttttc ggccgaggaa tgggggctct
    10741 acggcagctc cttttatgcc caaagggcca agtcccgcgg ggagcggatc gtcggcgtca
    10801 tcaacatgga catgatctcg tacacggact cggacgatcc cctcctcgag gtgtacgtca
    10861 atccggccag ccgctggctg gccgatcaat acctcgaggc ggccgagact tacgctccgc
    10921 tggcggcgtc caagatcacc gactcatcgg cgacgtggag cgaccactcg cctttttggg
    10981 accagggatt cgccgccctg tgcgccatcg aggagtcggc cggccggaat ccctactatc
    11041 acgagacgac cgacaccctg gacaagctga acatgaatta ttgcacgtcc gtgacccggg
    11101 cttcgctggc ctcggccgcc gacctggccc ggccgcatgt ggtccggccc gctccgccga
    11161 cggggcttca agcccggagc gagatcatca gctcgctgtt cgccgcggtc aaaagtgttt
    11221 cgctcgcctg ggacgccggc ccggcgtccg tcgccggcta caacgtctac cggacgacga
    11281 cgtccggcgg ggggcacgtg aagatcaatt cccgattgtt gagcgagccc cgtttctccg
    11341 aaacgatgct ggacccggcg acgacctact tctacgaggt cacggccgtc gacggccagg
    11401 ggctggagag cgatggctcc gacgaggttc gcgatgatca aaacaatgac gcgcttccga
    11461 attaaagccg gcttgcgggc ggcgcccgtt ttttggtggc tgattctcgg gatggggccg
    11521 gccggccttc gggcttcgac ggccgccgga cggccgcgca cggccgtatc gctggaggcg
    11581 gggatgttcc gggcgggcca ggcggctttc cggacgctgt atggaaacgg gatctccgcc
    11641 ttatcggctc atgtccgggt ccgcctcatc gacggcctct gggccgcggc cgggtggcgg
    11701 acggtcaaag ccgggggcgc gacggcgatc gccggaccgg ctttcaacga ggaagaatac
    11761 gccttggagt tcgagatgac ctcgatccgg ctcggcttgc gctatgcctt cgatctcgga
    11821 cggatcgccg ttttcgccgg agcggggggc agcgccgatc gctatgagga gcggtgggtc
    11881 gacgtccccg gcctcggcgg gagcggaaaa aaggcgggtt ggtacgccga ggcgggggga
    11941 gaatacgccg tctttcgctt tctctcgctt tccctccgcg gagaatacac gtccgttccg
    12001 acggccaggg gctcgtccct ggagccggac gtcaacttgg ggggcttgga ggccgccgcc
    12061 ggactggttt tccggttttg agaggagatc ttcagccgcc ggttgacgcg gccggggaaa
    12121 accgataaat gggggaagat ttcaaaaagc ctttgtcttt aatacgggcg taaagaggac
    12181 aaagatgaaa ataagaaaat gggcggttct ctattcgctg attgtcggcg tatccatgat
    12241 cggaatgtgg acgatgtttt ttgcatccgg cgggatcccg gagctcaaga ccaagcccgt
    12301 tgaaatcaac cttcatctcg ccgccgagtt atcgacggcc gttctgctcc tcatcggggg
    12361 ctgggggctg atcaagaggc ggccctgggg aactcaagcg cattccctct ccatggggat
    12421 gctgctctat acgttgatcg cgagtccagg ctattatctt caaaagggag agcttccctt
    12481 cgttttcata ggcctgtcct tctttaagaa agaagatttt ttaagcgagc ctcgccgccg
    12541 ggattaagcc cgaccccggc cgggatcgtt tccttattag gccgaacacc gccggcgggg
    12601 aaactcgctc gagaggtttt ccggggcggc tcgccgggag gctcgttttc atcgtcaccc
    12661 gcgttctccg cgtccttgac atcgattccg gcccgattta ttctttgcgg cgaaaggaag
    12721 aatggctttg agaaaaccgc ggccgggctg acgaggctcc gccgctttca ggccgggcct
    12781 ctccgggatt gtttcctcgg ggaaaagccg gcatacccct tcgggggcga tgaaagacaa
    12841 ggccgaagga ggatcggcgg ggccgctttc cgcgttcccg cgcccgcagg agacgggaga
    12901 gaccatgaag cgtttcaaga ccgcgcgccg ccccgctgtc cgccggttga gcccggcgcg
    12961 cctattcatt ctcagcttcc tgctggtcat cctgatcggc gccctgctcc tgctccttcc
    13021 cgcggcgtcc gtccgcgagc ctctttcctt aatggacgcc ctgttcacct cggcttcggc
    13081 ggtttgcgtc accggcctca ccgtcgtcga catcggccgg gatctgtccc tgttcggtca
    13141 agtcgtcacg ctcctgctct tccaaatcgg cgggctgggg atcatcacct tctcgctggt
    13201 cctgttcggg ctgatgggcc gcagcctctc gttccgcggc cgcgagctgc tgcaaagcac
    13261 gtttctccac acgccgcggc gtgatttttt ccgcatcctg cgcttcgtcg tgtcgggaac
    13321 cttcgtcatc gagggggcgg ggacgctggt ccttttccta cggttcctgt ccgattttcc
    13381 gccgggccgg gcgctgtacc aggcggtcta ccacgccgtg tcggccttca acaactgcgg
    13441 ctactccctg ttcgccgaca acctggcccg ctaccgcgac gacccggtgg tcaacctggc
    13501 gatcgtctcc ctgatcgtcc tgggcggcct cggcttcatc gtcatgtatg aggtcatcga
    13561 ccgctggcgc ggacggcaga agcgcctctc gctgcacgcc agggtggtgt tgctgaccac
    13621 cggggcgttg atcacgggcg gcgcgcttct gttctatctc ttcgaggccg gcaatgtcct
    13681 ggttggagtc cgcggacccg gccgcgtgct ggcttcgttg ttccagtcgg tgacgccccg
    13741 cacctgcggg ttcaataccg tcgacatcgc cgccttggcc aatcccacca tcctgctgat
    13801 gatgatcctg atgttcatcg gcgcctcgcc gggatcgacc ggcggcggga tcaagacatc
    13861 cagcttcgcc ctgctggggc tgatgatcat caaccgcatc cgcggccgcg acggcgtgac
    13921 catcttccgg cgcaccgttc cccaagagat cctcgggcgg acaatcgcca tcgtactggc
    13981 ctcggccgta tcggttctgg tcatcgcctc ggtcctgctg ctggccgccg ggcccggcgc
    14041 cggcccggac agccgccact tgttcgtcga atacatgttt gaaacgctgt cggccttcgg
    14101 aaccgtcgga ttgtcgatga acgtcaccgc cgccctgaac gaaatccaga agctggccat
    14161 cgtgctcatg atgttcatgg gccgcgtcgg accgctcacc ctggccttcg cctggtcgac
    14221 gttccggcgg ggactggtct atgccgagga aggcgtaatg gtcggatagg aggagccatg
    14281 aagcgctgcg tcgtcatcgg cctggggatt ttcggccaaa acatcgtccg cgagatttcg
    14341 gacaaaggat tcgaggtgat cgccgtggac aagaacaagg aggcggtgca gcgggtgcgc
    14401 gacctggccg ggaaagccat cgtcgccgac ggaaccgaca aggacctcat ggaccagatc
    14461 ggcatccatg atgaccgtgt cgtcctcatg gatgccgatc tggtccatga ggtccttg
//
LOCUS       DAOOXH010000060        14163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_4344_length_14163_cov_9.416785, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000060 DAOOXH010000000
VERSION     DAOOXH010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 14163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14163
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4344_length_14163_cov_9.416785"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /gene="groL"
                     /locus_tag="PKV64_07830"
     CDS             complement(<1..1137)
                     /gene="groL"
                     /locus_tag="PKV64_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259727.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPN17026.1"
                     /translation="MAKQILMGDEARHSMLKGVNTLSNLVKATLGPRGKNVVIDKKFG
                     SPTSTKDGVTVAKEVEIKDAYENMGAQLVKEVASKTSDVAGDGTTTATVLAQSIYGEG
                     LRYVTAGANPTLIKKGIDRAVEAVVAELKKISREVSGDMIAQVGAISANNDESIGKII
                     AEAMDKVGKDGVITVEEAKSMETALDIVEGMQFDRGYLSPYFITDPDRMECVLENALI
                     LLHEKKISNLRELLPLLESVARVGKPLVVIAEEVEGEALATLVVNKLKGTFMCAAVKA
                     PGFGDRRKAMLEDIAILTGGRVITEDIGVKLEKVDIAWLGEAKKVVIDKDNTTIVEGK
                     GKAADIQARIKQIRTQIDDTTSDYDREKLQERLAKIVGGVALIKV"
     gene            complement(1165..1452)
                     /locus_tag="PKV64_07835"
     CDS             complement(1165..1452)
                     /locus_tag="PKV64_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPN17027.1"
                     /translation="MKISPLYDRVLLKRIEEKEVKRGGIIIPDSAKEKPQEAEVIAVG
                     KGRTTDEGKVIPLTVKAGDKVLIGKYAGTEVTIDGEEHIILREEEILAVLA"
     gene            complement(1572..1805)
                     /locus_tag="PKV64_07840"
     CDS             complement(1572..1805)
                     /locus_tag="PKV64_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPN17028.1"
                     /translation="MDKKFDEMTIHQKMEILIKDMVEKEVRYRDALKEFQKIYLETAL
                     RKYRGNKTLMAKSLGLHRNTLHNKAKTLKIKKI"
     gene            complement(1789..2322)
                     /locus_tag="PKV64_07845"
     CDS             complement(1789..2322)
                     /locus_tag="PKV64_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPN17029.1"
                     /translation="MIGWLDIVLAVIIIAAVVIGLIKGLLRELIGLAAVVGGFILAAY
                     HYQKAADLLGGLVRGPLAARFLGFLLIFVLVVLAGALVTFLVSKLMKGTLRVLDHLMG
                     GVFGCLEGILIGGVLVFALLVFPVDRTAVSESRLAPYCYGLTKAAAALIPAELKNAAR
                     SAYQRIFKTEKSHGQEI"
     gene            complement(2319..3209)
                     /locus_tag="PKV64_07850"
     CDS             complement(2319..3209)
                     /locus_tag="PKV64_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPN17030.1"
                     /translation="MKSGFVDLHIHSDRSSDGDFSPDALARMAADTGFVAIAIADHDN
                     VAAYPDALEAGRREGVEIIPNMEVTAMYDDREFHCQLPFLAWDSPRVAAIVKRVAELR
                     WEEARRRIANLRSLGIGISWEEVTAASPDVAPLGVTIARILLDKPESRRDSRLRRYYD
                     EGGRPLPPSFFYRDFFLDGAPAYVPKRHIPLLEVVDEAARIGAVAVLSHPGAYFQRTT
                     HEDLVVLKDAGLAGLEVYTSYHDAGQVRFYLEEAKAFDLVPTAGSDFHGRVKPHVSFG
                     SIREGGYEMVEALRERRPSR"
     gene            complement(3206..4096)
                     /locus_tag="PKV64_07855"
     CDS             complement(3206..4096)
                     /locus_tag="PKV64_07855"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPN17031.1"
                     /translation="MNQPFADVILPELRLYKKGKVRDVYELDDKLLIVATDRISAFDF
                     VLPSLIPDKGRVLTQLSRFWFEYTSLVCPNHMIAADVAAFPPALAEYADLLDKRAMLV
                     RKTKVLPVECVVRGYLAGSGWKEYRASGKVCGIKLSDGLRESDQLPEPLFTPSTKAEA
                     GHDENISFKEMEKITGSVLAKKIRKVSLELYNKATLRALSKGIIIADTKFEFGLDGDD
                     LVLIDEIFTPDSSRFWPLASYEPGRGQPSLDKQFVRDYLETTGWDKTSDPPVLPADII
                     AKTSQKYLEIYRLLSEKTDL"
     gene            complement(4549..5232)
                     /gene="pgeF"
                     /locus_tag="PKV64_07860"
     CDS             complement(4549..5232)
                     /gene="pgeF"
                     /locus_tag="PKV64_07860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HPN17032.1"
                     /translation="MLFSIDERLSRIPGLVHGFGRGDLTEESLRTFALGRGLRLVLLD
                     QIHSDIIHIIEEAPAGRLPGDALATNAPGLLLAVKTADCLPVLLADFRLRVVAAVHAG
                     WRGTRLRIVEKTVRAMAGRFGTDPADVIAALGPGIGASCYEVGEEVRSEFLSAGLPGS
                     VFAPAGRPGKHFLDLAEANASLLRAAGVSEIAPAGDSGCTHCDPRMHSFRRDRDASRR
                     MFSFIGLSS"
     gene            5326..5532
                     /gene="rpsU"
                     /locus_tag="PKV64_07865"
     CDS             5326..5532
                     /gene="rpsU"
                     /locus_tag="PKV64_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPN17033.1"
                     /translation="MAFVVIDEGEPIESALKRFKRKVQQEAIIKEIKKHSVYYKPGER
                     KRMKEAMARKRMRRRMKRDKDLEY"
     gene            5529..6440
                     /gene="lipA"
                     /locus_tag="PKV64_07870"
     CDS             5529..6440
                     /gene="lipA"
                     /locus_tag="PKV64_07870"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPN17034.1"
                     /translation="MTVSDPPRPPASPKPAWLKVRLPDDEAYFAVSALLDKRGLHTIC
                     RSARCPNISECWAQKTATFLILGNVCTRACAFCAVTKGRPEAPEISEPERIAEAASLL
                     GLTYAVVTSVTRDDLPDGGAAHFAATIAALRRRIPGIRVEALIPDFGGNPNALDIVLA
                     VSPEILNHNLETTESLYPAIGRPASGYRRSLEVLRRAKERGFTTKSGLMIGLGETRDD
                     LLKTFRDLRQAGCDLLTIGQYLRPSPAHAPVVRYYPPEEFAGLKTLASAEGFPGVEAG
                     PLVRSSYHAQTLQASFERGRGDAACAP"
     gene            6428..7165
                     /locus_tag="PKV64_07875"
     CDS             6428..7165
                     /locus_tag="PKV64_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPN17035.1"
                     /translation="MRALIFSDVHGNLEALTAVLKYAKRHRIDQYICLGDLVGYGASP
                     NETIQKIQALKPLTVIRGNHDKAVAGQDSMETFNPIAAAAIQWTKSRILKKNLEFLAR
                     LPKGPLTIDKIEICHGAPFDEDFYIFGEYDAIEAFEYLPFPLSFFGHTHFPVIYSKHG
                     DQVAGRFLSGPVNLLKIEKGFSYLINPGSVGQPRDRNPRAAFAIYDTERLQVRFHRVE
                     YEIAEAQKKILQAELPAALAERLTLGL"
     gene            7169..7915
                     /gene="lptB"
                     /locus_tag="PKV64_07880"
     CDS             7169..7915
                     /gene="lptB"
                     /locus_tag="PKV64_07880"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPN17036.1"
                     /translation="MSRLEARRLVKRYGRRIVVKGVDAVFESGEIVGLLGRNGAGKTT
                     FFQMIVGLLRPDAGAVYLDGENMTSLPTDRRARAGLTYLPQENSVFLKASVIDNLRLG
                     LELQPLSRAEREATAAALLEEFNLSPLAAQGADSLSGGERRRLEICRALLLQPKFLLL
                     DEPFTGIDPLTIREIQKTLLRLKERGIGIILSDHNVRDTFQIVDRAYLIDDGEILVMD
                     TPARLAANPKARERFLGTEFRFGEERAPGA"
     gene            complement(7912..10287)
                     /locus_tag="PKV64_07885"
     CDS             complement(7912..10287)
                     /locus_tag="PKV64_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17037.1"
                     /translation="MAVRRCPYCKAIIDESQKYCNNCGTQLLFSDEEGEEPIKGEKIV
                     DEDFPETGGLDEPPELIDEPRDEVDLDAEPGEERREIDLEKVIEGEEEIEDEAAAEDQ
                     SFVDLREAEPEESLPRAVPGKTPVIKLETDATGGFSENEIKFPLKFETGELEIPMPSH
                     PSFLSKKSASAKEKSVDLSRIPPGRRDHDTKFEIARLIADFEQRRRNYTAELGIEAAE
                     AFPRIPSGRRGRDAKSEITRLIADFEKRWRDFTTEIGTEAAGGPSRIPSGGKDRDTEI
                     EITRLIADFERQWRDVSSEIGTEAADALSRIPSGGKDRDSKIEITRLIADFERQWRDF
                     TLELRTKAAGAAFRVPPGGRDRDAKSEITGLIADFEKRWRDYAAERGAEAADDRASRG
                     GEAAEEPVVPPRRAEVGAPPAGGQIEKEVEAPRAEPAGDSAAEYEFGCPDEASAPPAE
                     ETPEEEPSSTDDRLPFLAGSLKKAIAEGLTAGLEADDGTRHPVFTTRDLEEEIGPPKP
                     PSPPLVEKDLIIEERSVEDVAAEAAEDAGLVPGEAAVAEAETAGEKPAEMSGFLQTGE
                     EALRSGPVRETAEPDWESGKTSVALETFFPPAPEGKPEEKLFDTEEAFAPLSTIGLPE
                     SVPSVSPPLSFEIPVSEDEIERELEAIAPRRQPEIPENRRPESPAVEVEEESRGGSFL
                     EEAGEKESEQAPAVPEIGEAKAPARAPVVRLGFFRRIKATIFDLLVVGLFWVVATVLA
                     AHFMSTPVLDLIATAAGPLAILYAVLLGVYLFLFLLFLGETPGGRLVTPRN"
     gene            10500..10572
                     /locus_tag="PKV64_07890"
     tRNA            10500..10572
                     /locus_tag="PKV64_07890"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10533..10535,aa:Val,seq:cac)
     gene            complement(10713..12806)
                     /locus_tag="PKV64_07895"
     CDS             complement(10713..12806)
                     /locus_tag="PKV64_07895"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPN17038.1"
                     /translation="MCYFVFMKNRPRGLGPALVLVMFGFRFPAPAVAVSPQADASKPA
                     AVYREWIDLVSYIITPTEKDVFFRLTSDRERNSFINLFWNLRDPSKGTPQNEYKEEHL
                     KRFQHANRYFIGSSRPGWKTDRGKYYILLGPPLNKVDVLTNGLRPVEIWEYMGDPDQG
                     LPTMFRIVFYKRSEADDYRLYVPAVDGPYALLSNPEAQIDPADNAAILSKIQEIDSTV
                     AEIALNLIPGNQSRMFSPSLQDQILLARIGDLPTLNINSSYATNFLSFKGFVETSATT
                     NYVGMTSDVVILKDPRLPLNFVHLALQPGRLSVERLADGRYYFNFNLVVTLKKDDRFI
                     LQYDKDFPVYMTQAELDGLFSQGVIIADRFPVVEGEYKLMAILRNSVNDEIGFMERSI
                     KIVEGNPALPRISDPLISYGQGAEQTDGYAPFRIMGRSVKIEPKQVFGLKDVITPYFL
                     VERREAKGDLRIRIEVESLNPEKPFFKEYAVLLPSDDPYFFFPKTLDPLPTGDYALRG
                     KIMGPDDTVLHVRQKIFQIAPHAEIPHAPVVSKIVKDKDASYYYVLLAKEYDEIGSVS
                     QADAAYRTALDRQPVPADAVKAYAAFLWKQGRYDDLLAAVEALESRPEEAFSYFSLKG
                     RALYRKQEYHAAVENLLEANKRYDSDVDTLNALGLSMIRTGAKEEAVKVLTASLRLRA
                     EQPEIREILLRLEKK"
     gene            12895..13875
                     /locus_tag="PKV64_07900"
     CDS             12895..13875
                     /locus_tag="PKV64_07900"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN17039.1"
                     /translation="MKVTIMKRTLAVLFTVVLAAAGVSSLSGQEGQGTGRVRGTVTDE
                     ENRPLADVKILMKSTSYHFQRETLSNQKGKWAMMGFSKDVFDFTFSKEGYETGRAQVF
                     LSGANRNPDQDIVLKKIEQRPIQGLDNPADRETIRKASDFFEAGQYAEAMSLFKEFVE
                     SHPDLYLARVNLAKTLAKLKQYPEAIAEYQKVEEALKADPAVAQVEARLADIYVAMAE
                     TYQDQGLFDQTCAYYLKAIELTPPVDPAVPFNLAELLFSNQRVDEAIRYYQLAIQLKP
                     DFGLYELKLGYAFLNQGEMSSAAEHLKKFLALSPDDPQADSVRKILETIK"
BASE COUNT         2795 a         4319 c         4187 g         2862 t
ORIGIN      
        1 gaccttgatc agggcgacgc cgccgacgat cttggccagc cgctcctgga gcttctcccg
       61 gtcgtaatcg gaggtggtgt cgtcgatctg ggtgcggatc tgcttgatcc gggcctggat
      121 gtcggccgcc ttgcccttgc cctcgacgat cgtggtgtta tccttgtcga tgacgacctt
      181 cttcgcttcg ccgagccagg cgatgtcgac cttttcgagc ttgacgccga tgtcctcggt
      241 gatgacccgg ccgcccgtca gaatggcgat gtcctcgagc atggctttgc ggcggtcgcc
      301 gaagccgggg gccttgacgg cggcgcacat gaaggtgccc ttgagcttgt tgacgacgag
      361 ggtggccagg gcttcgccct cgacttcctc ggcgatgaca acgagcggtt tgccgacgcg
      421 ggcgacgctt tcgagcagcg gcaggagctc gcggaggttg gagattttct tctcgtgaag
      481 aagaatcaag gcgttctcga ggacgcactc catccggtcg ggatcggtga tgaagtaggg
      541 ggacaggtag ccgcggtcga actgcatccc ctcgacgatg tccagggccg tttccatgct
      601 cttggcttcc tcgacggtga tgacgccgtc cttgccgacc ttgtccatcg cctcggcgat
      661 gatcttgccg atggattcgt cgttgttggc ggaaatggct ccgacctggg cgatcatatc
      721 gccggagacc tcgcggctga tcttcttcag ctcggcgacg acggcttcga cggcccggtc
      781 gatgcccttc ttgatcaggg tcgggttggc cccggccgtg acgtaacgga ggccctcgcc
      841 gtagatggac tgggccaaga cggtggcggt cgtggtgccg tcgccggcca catccgacgt
      901 cttggacgcc acttccttca ccagctgggc gcccatgttc tcataagcgt ccttgatctc
      961 gacttccttg gccacggtca cgccgtcctt ggtgcttgtg ggggagccga acttcttgtc
     1021 gatgacgacg ttcttgcccc gggggccgag ggtcgccttg accaggttgc tcagggtgtt
     1081 gacgcccttg agcatcgaat gccgggcttc gtcgcccatt aaaatttgtt tggccatgtg
     1141 tgttctcctt ggatttgcgc cgaatcaggc gaggaccgcc aggatctctt cttcgcgcag
     1201 gatgatgtgc tcttcgccgt cgatcgtcac ttcggttccg gcatacttgc cgatgagaac
     1261 cttgtcgccg gccttcaccg tgaggggaat cactttgcct tcatcggtcg tccgcccctt
     1321 gccgacggcg atgacttccg cctcttgggg cttttccttg gccgaatccg ggataatgat
     1381 gccgcctctc ttgacttcct tttcctcgat ccgcttcaga agaacgcggt cgtacaacgg
     1441 ggaaatcttc atcaaaaacc tccttgaaaa gtgtaaatta gcaatctcaa aatgagactg
     1501 ctaatttaca actcggaatg gtcgttgtca agaggaaaaa tgaaaaaatt agcagacttg
     1561 agcgttggct gctaaatttt cttgattttc aaggtttttg ctttattgtg gagcgtattc
     1621 cggtggaggc ccaacgattt ggccatcaac gtcttgttcc ccctgtattt ccgaagggcc
     1681 gtttcgagat agattttctg aaattccttc agggcgtcgc ggtaccggac ttccttctcg
     1741 accatatcct taatcaggat ctccatcttc tgatggatcg tcatctcgtc aaatttcttg
     1801 tccatggctc ttctcggttt taaaaatcct ctggtatgcg ctccgagccg cgtttttgag
     1861 ctccgcggga atcagggcgg cggctgcttt cgtcagccca tagcaatacg gcgccagcct
     1921 cgactcggac accgccgtcc ggtcgaccgg aaacacgagg agggcaaaga ccaggacccc
     1981 tccgatcaaa atcccttcca ggcagccgaa tacgccgccc atcaggtgat caagaacccg
     2041 gagcgtcccc ttcatcagct tcgagacgag gaacgtaacg agcgccccgg ccaggacgac
     2101 aagcacgaaa atgaggagaa atcctaaaaa tcgagcggcc aaggggccgc ggacgagccc
     2161 gcccagcagg tcggccgcct tctggtagtg gtaggccgcc aagatgaatc cgccgacgac
     2221 cgcggccagg ccgatgagct cccggagcag ccccttgatc aagccgatga cgacggccgc
     2281 gatgatgatg accgcaagga cgatatccaa ccatccgatc atctcgacgg tctcctttcc
     2341 cggagcgcct cgaccatctc gtatcccccc tcccggatgg agccgaacga gacatggggt
     2401 ttcacccggc cgtgaaaatc cgacccggcc gtcgggacca agtcgaacgc ttttgcttct
     2461 tcgagataaa accggacttg gccggcgtcg tgataggacg tgtagacctc gaggccggcc
     2521 aggccggcgt ccttcagcac gaccaggtcc tcatgcgtcg ttctctggaa ataggccccc
     2581 gggtgggaca aaacggcgac cgctccgatc cgggcggctt cgtccacgac ctcgagaagc
     2641 gggatgtgcc gcttcggaac gtaagccggc gcgccgtcga ggaaaaaatc ccggtaaaaa
     2701 aacgacggag gcagcggccg tccgccctca tcataatatc tgcgcagccg ggagtcgcgg
     2761 cgcgattccg gcttatcgag gagaatgcgg gcgatggtca cgccgagggg agccacgtcc
     2821 ggggacgccg ccgtcacttc ctcccaggag atcccgatcc ccagcgaccg gaggttggcg
     2881 atcctccggc gggcttcctc ccagcgcagt tcggcgactc gcttcacgat cgcggcgacg
     2941 cgggggctgt cccaggcgag gaacgggagc tggcagtgaa actcccggtc gtcgtacatc
     3001 gccgtgactt ccatgttcgg gatgatttcc acgccttccc ggcgccccgc ctcgagggcg
     3061 tcgggataag cggcgacgtt gtcgtgatcg gcgatggcga tcgcgacgaa tccggtgtcg
     3121 gcggccatcc gggccagcgc gtccggcgaa aaatcaccgt cgctcgaccg gtccgagtgg
     3181 atgtgaaggt cgacaaaacc ggatttcaca ggtcggtttt ctccgaaaga aggcggtaga
     3241 tctccaggta tttttgggac gtcttagcga tgatatcggc cgggagaacc ggcggatcgg
     3301 acgtcttgtc ccaaccggtc gtctccaggt aatcgcgaac gaactgcttg tccagcgacg
     3361 gctggccccg gcccggctcg taggaggcca gcggccaaaa ccgcgaggaa tccggagtga
     3421 agatctcgtc gatgagcacg agatcgtcgc cgtccaggcc gaactcgaat ttcgtgtcgg
     3481 cgatgatgat gcccttggaa agagcgcgga gcgtcgcctt attgtagagt tccaggctga
     3541 ccttgcggat cttcttggcc aggacggacc cggtgatttt ctccatttcc ttgaaggaaa
     3601 tgttttcgtc gtggccggcc tcggccttgg tcgagggggt gaacagcggc tccggaagct
     3661 gatccgattc ccgcagaccg tcggaaagct tgattccgca gaccttgccc gacgcccggt
     3721 attccttcca gcccgatccg gccaggtagc cccggacgac gcattcgacc gggagaacct
     3781 tcgtcttgcg gacgagcatc gcccgcttgt cgagaagatc cgcgtattcg gcaagggcgg
     3841 ggggaaaggc ggcgacgtcg gccgcgatca tgtgattggg acagacgagc gacgtatatt
     3901 cgaaccagaa gcgggaaagc tgggtcagaa cccggccctt gtccggaatc aacgacggca
     3961 gaacgaagtc gaaggccgaa atccggtcgg tggcgacgat caggagcttg tcgtcgagtt
     4021 catagacgtc gcggaccttg ccttttttat aaagacgaag ttccgggaga atgacatcgg
     4081 caaagggttg gttcatggtg ctttttcctg atcgttaccg atctcgatgc agcggccatc
     4141 atacctaaaa gaaagaagcg attcaactgc acggggggag tgataataac gatcaatttg
     4201 atcgagacgc tttcacgtcc tcgggcccgt cggcttcgat tcccgcgttc cggatccggc
     4261 ggatcctaac gaaaaatcgc ggcgtcggcg ggaaccccga ggttctctca acaaccgtcc
     4321 gcctcttcgg agcgaatcgc gccctcctcc gtcgaccgcc tcagccgtcc cggcctccct
     4381 cgccgccggc gattttcatt tcctggatat gacccccttt tttcttcccg cttcggaaga
     4441 ggcggcggac aaaaggcgat ccgcggcatg atcaaacgag ttgtcttaat ggtttgacga
     4501 tttccgaaga ggaccgtcgc cgggaaaacg gcggaggatc ttttgtgatc aggaagaaag
     4561 gccgatgaag gaaaacatcc ggcgggacgc gtcgcggtcg cggcggaagg aatgcatcct
     4621 cgggtcgcaa tgggtgcatc cggaatcgcc ggcgggcgcg atctcggaaa caccggccgc
     4681 gcgcaaaagc gaggcgttgg cctccgccaa atccagaaaa tgcttgcccg gacggccggc
     4741 gggggcgaag acggagcccg gcagcccggc cgaaagaaac tccgagcgga cctcctcccc
     4801 gacctcgtag cacgaggccc cgatgccggg acccagggcg gcgatgacgt ccgccgggtc
     4861 cgtcccgaac cggccggcca tcgcccgaac ggttttctcg acgatccgaa ggcgcgtccc
     4921 ccgccagccc gcgtgaaccg cggccacgac ccggagccgg aagtccgcca ggagcacagg
     4981 gagacaatcg gccgtcttga cggccaaaag caggccgggg gcattcgtcg ccagggcgtc
     5041 gcccggcaag cggccggccg gcgcttcttc aatgatatga ataatatccg agtgaatttg
     5101 atcgagaagg acaagccgta acccccgtcc gagggcaaac gtccggaggc tttcctcggt
     5161 gagatccccc cgcccgaagc cgtggacaag gccggggatc cgggacaagc gctcgtcgat
     5221 cgaaaacaac atggaagcgg actcattata tccgatttta ttgacttttc ctcgcggacc
     5281 ggtataatct tggactttcc cgagacggga ggaggtgaat ccccagtggc cttcgtagtt
     5341 atcgatgaag gcgaaccgat cgagagcgcc ctcaagcgtt tcaagcggaa agtgcagcag
     5401 gaagcgatca tcaaggaaat caagaagcac tccgtctatt acaagcccgg agaacgcaaa
     5461 cgcatgaaag aggcgatggc tcggaaacgg atgcgccgtc gcatgaaacg cgacaaagat
     5521 ctcgaatatt gacggtttca gatcctcccc gccccccggc gtccccgaag ccggcctggc
     5581 tcaaggtcag gcttccggac gatgaggctt acttcgccgt ctccgccctg cttgacaagc
     5641 gcggcctcca caccatctgc cggagcgccc gctgtccgaa catcagcgag tgctgggccc
     5701 agaagacggc cacgttcctc atcctcggga acgtctgcac ccgggcttgc gccttctgcg
     5761 cggtgacgaa gggacgcccg gaggcgcccg agatctccga gcccgaacgg atcgccgagg
     5821 cggcctccct cctcgggctg acttacgccg tcgtcacctc ggtcacgcgc gacgaccttc
     5881 cggacggcgg ggccgcccat ttcgccgcga ccatcgccgc tcttcggcgg cggattcccg
     5941 ggatccgggt cgaggccttg attcccgatt tcggcgggaa cccgaacgcc ctggacatcg
     6001 ttttggccgt ttcccccgaa atcctcaacc acaacctcga aacgaccgag tccctctacc
     6061 cggcgatcgg ccgtccggcg tctggatacc gccgatcgct cgaggtcctg cgccgggcca
     6121 aggaacgcgg atttacgacg aagtcgggcc tgatgatcgg gctcggcgaa acgcgggacg
     6181 atttgctgaa aaccttccgc gacctccgcc aagccggttg cgacctcctg accatcggcc
     6241 agtatcttcg gccgtctccc gcccacgccc cggtggtccg ctactacccg cccgaggaat
     6301 tcgccggtct caaaaccctg gcctcggccg aaggattccc cggcgtcgag gccggcccgc
     6361 ttgtccgcag ctcctaccac gcccagaccc ttcaggcttc gttcgagcgg ggccgcggag
     6421 acgccgcatg cgcgccctga tcttcagcga cgtccacggc aacctcgaag ccctgaccgc
     6481 cgtcttgaaa tacgccaagc gtcaccggat cgaccaatac atttgcctcg gcgatcttgt
     6541 cggctacggg gcctcgccca acgaaaccat ccagaagatc caggcgctca agccgctcac
     6601 cgtcatccgg gggaaccacg acaaggcggt cgccggccag gactcgatgg agacgttcaa
     6661 tcccatcgcc gcggccgcga tccaatggac caagtcccgg attctcaaga aaaacctgga
     6721 attcctcgcc cgcctcccca agggtccgct gacgatcgac aaaatcgaaa tctgccacgg
     6781 cgcccccttc gacgaggatt tttacatctt cggggaatat gacgcgatcg aagcgtttga
     6841 ataccttccg ttccccctgt cgtttttcgg ccacacccat ttccccgtca tctactccaa
     6901 gcacggagat caggtcgccg gccggttcct ttccggcccc gtcaacctgc tgaagatcga
     6961 aaagggcttc tcctatctca tcaatccggg atcggtgggc cagccgcgcg accgcaatcc
     7021 ccgggccgcg ttcgccatct acgacacgga gcgcctccag gtccgtttcc accgggtgga
     7081 gtacgagatc gccgaggccc agaaaaaaat cctccaagcc gagctccccg ccgccctggc
     7141 ggagcggctc accctcgggc tctgaccgat gtcccgcctc gaagcccgcc gcctggtcaa
     7201 gcggtacggc cggcggatcg tggtcaaagg cgtggacgcg gttttcgagt ccggcgagat
     7261 cgtcggcctg ctgggccgca acggagcggg aaaaacgacc ttcttccaga tgatcgtcgg
     7321 cctgcttcgc cccgacgcgg gggccgtcta tctggacggc gaaaacatga cctctctccc
     7381 gacggaccgg agggcgcggg cgggattgac ctaccttccc caggagaact ccgtttttct
     7441 caaagcctcg gtcatcgaca atctccggct cggcctggag ctgcagccgt tgtcgcgggc
     7501 cgagcgggag gcgacggcgg ccgcgcttct cgaggaattc aacctctccc cgctggccgc
     7561 ccaaggggcc gacagcctgt ccggcggaga gcgccgccgg ctcgagatct gccgggccct
     7621 ccttctccag ccgaagttcc ttcttctcga cgagccgttc accggaatcg atccgctgac
     7681 catccgcgag atccaaaaaa cgctcctccg cctgaaggag cggggcatcg ggatcatcct
     7741 ctcggaccac aacgtccggg acacgttcca gatcgtcgac cgggcttacc tcatcgacga
     7801 cggggagatc ctggtgatgg acacgcccgc ccggctggcc gcgaacccga aggccaggga
     7861 acggtttctc gggacggaat tccggttcgg cgaagagcgg gcccccggcg cttaattccg
     7921 gggcgtcacc agccgccctc cgggcgtctc tcccaaaaac aaaaggaaga ggaaaagata
     7981 caccccgagt aggacggcgt agaggatcgc cagcggtccg gcggccgtgg cgatcagatc
     8041 gagaaccggc gtcgacataa aatgggcggc caggacggtc gcgaccaccc agaaaagccc
     8101 gacgaccagc aggtcgaaaa tcgtcgcttt gatccgccgg aaaaatccca gccggacgac
     8161 cggcgcgcgc gcgggcgcct tcgcttcccc gatttccggg acggcggggg cctgttcgga
     8221 ttccttctct cccgcttcct cgagaaagga tccgccccgg gattcttcct cgacctcaac
     8281 ggcgggggat tccggacgac gattttccgg aatttcgggc tgtcggcgcg gagcgatcgc
     8341 ctcgagctcc cgctcgatct cgtcctcaga gaccgggatt tcaaaagaca ggggcggcga
     8401 gacggaaggc accgactccg gcaaccctat cgtcgaaagc ggcgcgaatg cctcttccgt
     8461 atcgaagagt ttttcctccg gctttccctc aggcgccgga ggaaaaaacg tttccagggc
     8521 gactgaagtc ttgcccgatt cccagtccgg ctcggccgtt tcccgtaccg ggcccgaacg
     8581 gagggcctcc tccccggtct gaagaaaccc ggacatctcg gccggctttt cgccggcggt
     8641 ttccgcctcg gcgacggctg cttccccggg gacaagaccg gcgtcctccg ccgcttccgc
     8701 ggcgacgtct tcgacgcttc gctcctcgat gatgagatcc ttttccacga gaggcggact
     8761 cggcggcttc ggcggtccga tttcctcttc aagatccctc gtggtaaaaa cggggtgccg
     8821 cgtgccgtca tccgcctcca agccggcggt cagcccctcg gcgatcgctt ttttcaagct
     8881 tccggccagg aacggcagac ggtcatcggt tgaagacggc tcctcctccg gcgtctcctc
     8941 ggccggggga gccgaggctt cgtccggaca gccgaattcg tattccgcgg cggaatcccc
     9001 cgccggctcc gcccggggcg cttcgacttc cttttcgatc tgtccgccgg ccggcggagc
     9061 gccgacttcc gccctccttg gagggacgac cggctcctcg gcggcttctc ctccccttga
     9121 agcccggtca tcggccgcct cggccccgcg ctcggcagca tagtcccgcc accgcttctc
     9181 aaaatcggcg atcagtccgg tgatttcgga tttcgcgtcg cgatcccgcc cgcccggggg
     9241 aacccggaaa gcggcgccgg cggctttcgt cctgagctcc aaggtaaagt cgcgccactg
     9301 ccgttcgaaa tcggcgatca ggcgggtgat ttcgattttc gagtcccgat ccttcccgcc
     9361 cgagggaatc cgggaaaggg catccgccgc ctccgtcccg atctccgatg aaacgtcgcg
     9421 ccactgccgc tcgaagtcgg cgatcaggcg ggtgatttcg atttccgtgt cgcggtcctt
     9481 cccgcccgag ggaatccggg aggggccgcc ggccgcctcc gtcccgatct ccgtggtgaa
     9541 gtcgcgccac cgcttctcga aatcggcgat caagcgggtg atttcggatt tcgcgtcgcg
     9601 ccccctccgt cccgacggaa tccgggggaa ggcctcggcc gcctcgatcc cgagctcggc
     9661 cgtataattg cgccgccgct gctcaaaatc ggcgatcagc cgggcgattt cgaatttggt
     9721 gtcgtggtcc ctccggcccg gcggaatccg ggagaggtcg acgctcttct ccttggccga
     9781 ggccgatttt ttcgaaagga acgacggatg ggagggcatg gggatctcaa gctcgcccgt
     9841 ttcgaatttc agggggaact tgatttcgtt ctcggaaaaa ccgcccgtcg cgtccgtctc
     9901 cagcttgatg acaggcgtct tcccgggaac cgctcggggg aggctttcct cgggttcggc
     9961 ttcccgcaaa tcaacgaaag attgatcctc ggcggccgcc tcgtcctcga tttcctcttc
    10021 tccctcgatg accttttcaa ggtcgatttc ccgccgctcc tcgcccggct ccgcgtcgag
    10081 atcaacctcg tccctcggct cgtcgatgag ctcgggaggc tcatcgaggc cgcccgtttc
    10141 gggaaagtcc tcgtcgacga ttttctcccc cttgatgggc tcctcgccct cctcgtcgga
    10201 gaaaagaagc tgggttccgc agttattgca gtatttctgc gactcgtcga tgattgcctt
    10261 gcaataagga cagcgcctga cggccattaa gcaaattcct acgatattta taaagggatt
    10321 aaggaaggat gtcaacgttc caccggggac ggaagaaggg ccgggatcag gagaaaatga
    10381 ggcaaaatcg cggcgaaggg agcggaggga aacgaggcgg cccgggggaa aatagcggaa
    10441 acggcccgga tcaggggaaa ttgactttcg agggcgattg gccgaaaata tgacccgctg
    10501 ggcgattaac tcagtggcta gagtactacc ttcacacggt aggagtcgga ggttcgaatc
    10561 ctctatcgcc catttttttc atcgacgttg tcttcttttg caagctttat cttcgtggat
    10621 cgccgtcgtg aacgcggggc agtgatatcc aaaccattct gaaacggctt tgttgatatc
    10681 gcccgcggag gcctttccga aaagaaatcc tgttacttct tttccaaccg gagaagaatc
    10741 tctcggattt ccggctgctc cgcccgaagc ctgagcgagg ccgttaaaac tttgacggct
    10801 tcctcttttg ctcccgtccg gatcatagac aggcccaggg cattcaacgt gtcgacgtcg
    10861 ctgtcgtatc tcttgttcgc ttcaagaagg ttttcgacgg cggcgtgata ttcctgtttg
    10921 cggtaaagcg cgcgtccttt caagctgaaa taggaaaaag cttcctccgg ccgggactcg
    10981 agggcttcga ccgcggccag gagatcgtca tatcggccct gcttccagag aaaggcggca
    11041 taggccttga cggcatcggc cggcaccggt tgccggtcca gggcggttcg atacgcggcg
    11101 tctgcctggg acaccgagcc gatttcgtcg tattccttcg ccaggagaac atagtaataa
    11161 gacgcgtcct tgtctttgac gatcttggag acgacaggag catgaggaat ctcggcatga
    11221 ggcgcgattt gaaaaatttt ctgccggacg tggaggaccg tatcgtccgg tcccatgatc
    11281 ttccctctca aagcgtagtc tccggtgggg agagggtcca gggttttcgg gaaaaagaag
    11341 taggggtcgt ctgaagggag aaggacggcg tactccttga aaaaaggctt ttcggggttc
    11401 aagctttcga cttcgatcct gatccgaagg tcccctttag cctcccggcg ttcgaccagg
    11461 aaatacggcg tgatgacgtc tttgagtcca aagacctgtt tgggctcgat cttcacgctc
    11521 cggcccatga tcctgaaggg ggcatagccg tcggtttgct cagcgccttg accgtaggag
    11581 atgagcggat cggagatccg gggaagggcc ggattccctt cgacgatttt gatcgatcgc
    11641 tccatgaagc cgatttcatc attgacggaa ttgcggagga tcgccatgag cttgtattcg
    11701 ccttcgacga cagggaagcg gtcggcgatg atgactccct gggagaagag cccgtccagc
    11761 tcggcctggg tcatgtaaac ggggaagtct ttatcgtact ggaggatgaa gcggtcgtcc
    11821 tttttcaggg tgacgaccag attgaaattg aaataatacc ggccgtccgc gagacgctcg
    11881 acggagagac gcccgggctg cagagccaga tggacgaaat tcaagggcag gcgcggatcc
    11941 ttgaggatga ccacgtcgct ggtcattccg acataattcg tggtggcgct ggtttcgacg
    12001 aatcccttga aactcaggaa gttcgtggca taggaagaat tgatgtttaa agtcggcaga
    12061 tcgccgatcc gcgccagcag gatctggtcc tggagggaag ggctgaacat gcgggactga
    12121 ttcccgggaa tcagattcag ggcgatttcc gcaaccgtcg aatcgatctc ctggattttg
    12181 gagagaatgg ccgcgttatc ggcggggtcg atttgggctt ccgggttgga cagcagcgcg
    12241 tacggaccgt cgacggcggg aacgtagagc cggtagtcgt cggcctcgga gcgcttgtaa
    12301 aacacgatcc ggaacatcgt cggcaatccc tgatccggat cgcccatgta ttcccagatt
    12361 tccacgggcc gcaatccgtt ggtcaaaaca tcgactttat tcagcggcgg tcccagaaga
    12421 atgtaatatt ttccccggtc cgttttccag ccgggccgcg aggatccgat gaagtaccgg
    12481 ttcgcgtgct ggaaccgttt gagatgctcc tctttgtatt cattttgtgg ggtccccttg
    12541 gacgggtccc gcagattcca gaagaggttg atgaaggaat tccgttcccg gtcgctggtc
    12601 aaacggaaaa agacgtcctt ttccgtcgga gtgatgatgt aggagaccag gtcgatccat
    12661 tcccggtaga ccgccgccgg ttttgaggca tccgcctgcg gggaaacggc aaccgccggc
    12721 gccggaaaac ggaatccgaa catgaccaga acgagcgccg gaccgaggcc gcgcggacgg
    12781 tttttcatga aaacaaaata acacactcca cgctctttcc tcaacgggcg ccgccctcgt
    12841 ttgagaacgg gaatccaatt tgataacatg gaatcttcag aaaaacgctt tggaatgaag
    12901 gtgaccatca tgaaaagaac cctggcggtg ctgtttacgg tcgtcctcgc cgcggcaggc
    12961 gtttcttccc tttccgggca agaaggccag ggaacgggaa gggtcagggg gaccgtcacg
    13021 gacgaagaga atcgccccct ggccgatgtg aaaatcctca tgaaatcgac cagctatcat
    13081 tttcaaagag aaaccctcag caatcaaaag ggtaaatggg cgatgatggg attctccaag
    13141 gacgttttcg atttcacctt ttccaaggag ggttacgaga ccggccgggc ccaggttttt
    13201 ctctctggag cgaaccgcaa tccggatcag gacatcgtcc tgaaaaaaat cgagcaacgc
    13261 cccattcagg gactggacaa tccggcggat cgcgaaacga tccggaaagc gtccgatttt
    13321 ttcgaggcgg gccaatacgc ggaagccatg tccttgttca aggaattcgt cgagagtcac
    13381 cccgacctct acctcgcccg ggtcaatctg gctaaaaccc tggccaagct caaacagtat
    13441 ccggaggcga tcgccgaata tcaaaaagtc gaggaagcct tgaaggcgga tccggccgtc
    13501 gcccaagtcg aggcgagatt ggcggatatt tacgtggcca tggccgagac ctatcaggat
    13561 caaggtcttt tcgatcaaac ctgcgcctat tacctgaagg ccatcgaatt gacgccgccc
    13621 gttgatccgg ccgtcccctt taatctggcc gagcttcttt tcagcaacca gcgcgtggac
    13681 gaggccatcc gctattacca gctcgcgatt cagctgaagc cggatttcgg cctttatgag
    13741 ctgaaattgg gctatgcttt tctcaaccag ggcgagatgt cttccgccgc ggagcatctg
    13801 aaaaaatttc tagccctatc gccggatgat cctcaagccg attccgtgag gaaaatcctc
    13861 gagacgatca aataaaagac gatccctctc cgccggatct tgggggatct cttcaagatc
    13921 tatacgctaa cggaagcatc ttccgacatc aaaagtcatc ggttcgtgag gccaccgagc
    13981 agccggacga tattcaaaat tttgaatatt ttacaaaatt ttgaatccgc atttatcatt
    14041 ttaacttttt catctttatt atcttattga tattaaatta attaatattt attaagcatt
    14101 tctggtattt ggcacaatat atgctatata ttgcgtgtaa tatatctacc tgatatatcg
    14161 aag
//
LOCUS       DAOOXH010000061        14100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_4376_length_14100_cov_7.361339, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000061 DAOOXH010000000
VERSION     DAOOXH010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 14100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14100
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4376_length_14100_cov_7.361339"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PKV64_07905"
     CDS             <1..641
                     /locus_tag="PKV64_07905"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HPN17040.1"
                     /translation="LLGLFLLARPLYITIFRIQTESIKNLTVLGLIGAGAATVLLAVS
                     LGFIFFRDKALALTGAVLRARIIPERFRSKVLELLHEFIDGLKFFQSLKTLLAYIGLS
                     FVVWLGIVFFYWVFFLAFDIRIRFFFLFPYVFLTGVGAAIPTPGMVGGYHAFSKQGMI
                     ELFGMNANEAGGLTVVFHAVQYIVSCLVGLIFLWIDGLTLIQIKRLGKAKTP"
     gene            638..1093
                     /gene="nrdR"
                     /locus_tag="PKV64_07910"
     CDS             638..1093
                     /gene="nrdR"
                     /locus_tag="PKV64_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPN17041.1"
                     /translation="MKCPFCGHEESKVIDSRESKKGISIRRRRECIACGRRFTTYEKI
                     EEIPYMIVKKDGSRQLFDRQKLLRGLLKACEKRPIPVSKFEEIVEEVESKLQERPEKE
                     MKASEIGEMVMARLKDLDKVAYVRFASVYREFRDVLEFKQELESLLREK"
     gene            1151..1567
                     /locus_tag="PKV64_07915"
     CDS             1151..1567
                     /locus_tag="PKV64_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPN17042.1"
                     /translation="MIKRLHPVIRIRTSGGGVYPADWQGGWMPRLDLIETEKTLIVAV
                     EVSGLRTEDLQISLQTNRLEIKGRKKEAAVPAGTRYFRLEREYGPFRRTLALPRSVVP
                     DRARATLENGVLTIALPKRIETPRRRRLVRIKRNVE"
     gene            1573..3969
                     /gene="lon"
                     /locus_tag="PKV64_07920"
     CDS             1573..3969
                     /gene="lon"
                     /locus_tag="PKV64_07920"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPN17043.1"
                     /translation="MAEKDPQSDPVDGLADGQDSKPSIPVKLPILLLRDIVVFPYMIV
                     PLFVGREKSKAAIDEALASHRMILLLTQRDVDTEDPKQDEVYQMGTVALIMRMLKLPD
                     GRIRILAQGLTRAAVRTYDEENGHFIAEIEPIPEPEDQEKSLEGEALVRNVRSGLEKA
                     ASLGKSIPPEVLIIASNVEEPGRLADLTGANLELKVPEAQSILEIVDPIARLKKVHDD
                     LTKEIEILDVQSRISTEAKGEMDKHQRQYFLRQQMKAIQKELGEGNELQEEIRSFQEK
                     LKKLKVTEEVRGEIEKNISRLSQMHPESAETTVVRNYLDWMFALPWSKSTIDNLDLKK
                     AKAILDEDHYGLDKVKERIIEYLAVRKLSRRIKGPILCFVGPPGVGKTSLGKSIARAL
                     GREFLRISLGGVRDEAEIRGHRRTYVGALPGRIIQGLRRAGSNNPVFMMDEVDKIGAD
                     YRGDPSSALLEVLDPEQNNTFRDHYLAVPFDLSRVMFITTANLLDPIQPAFRDRMEII
                     QLPGYTEEEKMEIAARHLVPKQIKENALSDKYIQVTPGAIRTIISRYTREAGVRNLER
                     EIASICRKVARKVAENKKTLNKITSQSVESYLGPPKIFKDELRKSDRVGVATGVAWTE
                     TGGEVLFVEALKMKGKGGLMLTGLLGDVMKESAQAALSYARVHTKELKIDNRIFGTND
                     FHVHIPEGAIPKDGPSAGITIAAALISLCTNRAVRWDTAMSGEITLQGNVLPVGGVKE
                     KVLAAQRAGVKRMILPSPNRKDLIDIPKVVKKEMEFIFVEDIRQVLEAALVRKTGRPT
                     "
     gene            complement(3962..4816)
                     /gene="dapF"
                     /locus_tag="PKV64_07925"
     CDS             complement(3962..4816)
                     /gene="dapF"
                     /locus_tag="PKV64_07925"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPN17044.1"
                     /translation="MKFTKFHALGNDFLIVDQDEVALFEEPGDLAHRLCERHTGVGAD
                     GILLVSVTNRSRGEARFRIFNADGSEAEISGNGLRCAAAYLYSQQKIDVPVVRFQTAA
                     GERTCALVRRTKDGFLMKIELGIPRFAPADIPFDDGTVHDRILDYPLVINTETYPVTA
                     VSLGNPHCDVFIDRFPGRFEWHELGREIESHPFFPRRTNIEFIRVRGRDEIEVVFWER
                     GVGETLSSGSGAAAAAVAAIVTGRTDRTVTVRTEMGSLLVEWPSETAPVVQTGPAEFA
                     FVGSFLFK"
     gene            complement(4819..6336)
                     /locus_tag="PKV64_07930"
     CDS             complement(4819..6336)
                     /locus_tag="PKV64_07930"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HPN17045.1"
                     /translation="MTKRSAWIAAAAFVLGVLAAGYVFVFLPEKKTEARSFLSGDTRS
                     LGTNLFAETPPIRPDLDFVAVSEKIGPTVVRIDAERVEPGMTGRVTPFDDFWDRFFGS
                     PGQRPRSQTPQESRAIVQGTGFFISPDGYILTNNHIVEKAKKISVFTVAGDEYEANII
                     GTDVLTDLALIKVEGKDLPFAELGDSTALKVGEWVLAVGNPLGMEHTVTAGIVSAKGR
                     QLGLGDSRTYEDFIQTDAAINRGNSGGPLVNLKGLVVGINSNILTPSGGNIGIGFAIP
                     ASMAKKVVIQLKDKGRVVRGRLGVSIAIRPIDQDTQKALNLKDRKGAVINDVEDGTPA
                     EKAGLKRYDVIIGVNGQPIENSNDLRFKISEIPPGSPVEIRFIRDGKEMTVTTKVIEL
                     APEEKSRPAAGAAPKNIGLSVEALTPPLARRYGLKTDRGLLVTDVAPGGPADRRGLAP
                     GDIIIEANRKKIETLDQWNAMIKPLKPGDPLMLAVRREDDSGQFSEFIVTLRITD"
     gene            6534..7133
                     /gene="tmk"
                     /locus_tag="PKV64_07935"
     CDS             6534..7133
                     /gene="tmk"
                     /locus_tag="PKV64_07935"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012939752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPN17046.1"
                     /translation="MKKGRLIVFEGIDGAGKSTQVRMLKRKLRARGRDVAAFREPSRS
                     RFGRTLRAKAKEAGSLTPAEELDLFIKDRRENVARNIRPALRDGRVVLLDRYYFSTMA
                     YQGAKGLDPEWIRTVNERFAPKPDLVFILDVGPPEGLARISRRGTRDALFERESYLRK
                     VRRLFRTFQGPRFVHLDGKRDKRDLGREILDRTLAVLGE"
     gene            7140..9587
                     /locus_tag="PKV64_07940"
     CDS             7140..9587
                     /locus_tag="PKV64_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HPN17047.1"
                     /translation="MAAARKGLKIIFRRNPRKAGAGSRRKRAWKVVLWAAFVGTALLF
                     GAVFGTFVAVRRTLPDLSGLESYEPALSTKVLADDGQVVREIGVEKRTIVTYEQIPAV
                     LRDAILATEDPHFFKHRGVDVRGILRSVKENALNVFRRRKLHGGSTITQQVARKLFLH
                     PLQTLQRKFAEWYVSIQIEKKYSKDRIFEMYCNQFELGPGAAGVEAASRLYFGKSVGD
                     LILEEAALIAGLYRGPTLYSPYRNPALALERRNHVLTRMAEEGMITAARAEDGRKTPV
                     AVLPPGREDYDFGAYFFEEVRRAIAEKYGEEALYSGGLKVSTTLNAAWQKFADEAVQN
                     GLRAYDKRHGWRRDKANLLADEAFKASGKKLEEYRLRSWVTPRLEPGDIEEAIVLSVG
                     RKEVRIKVKDDFARLINEGAEWAAPGKALDSLLKPGDVIQVRVKSRDAEKKETVATLE
                     QEPLAQAALVAIDPRTGQIKAMVGGSSFRRTQLNRATQTSRQAGSAMKPFIYTAALER
                     GFTAASRLVDEPTDFPDPWSGTVWTPRNYDRKYKGMITFRTGLEESRNVITAKILDNI
                     SPQTGVDYAKRFGLTGTLYPYLSLALGTFEVNLVEMVSAYGVFPNKGIRIRPFFVSRV
                     EDRDGNLLEEHVVEAEEVVSPQTAFLMTHLMEGVVQRGTAAAASVFLNDTALGGKTGT
                     TDDCTDAWYIGYSPSLCVGVWVGMDDNTSLGRNETGAVAALPIWMTFFGRVLEEIKAK
                     AKDASVEYYPEEFETPPNIHFMAIDRKTGLLATPQCKWRFMEAFLEGTEPTRLCSMLD
                     HISTLDYGGTSVAREER"
     gene            complement(9615..11390)
                     /locus_tag="PKV64_07945"
     CDS             complement(9615..11390)
                     /locus_tag="PKV64_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17048.1"
                     /translation="MRKTALAAAAVLLFFTWASGQDAVPPDSAGREAAGGRYSYARLS
                     FVDGRIFLQRAADLASEEATINTPISEGDRLGTDEGRAEISFGDIKYARLDHYTKIDV
                     QTLPRDHSSLLRLRHWAGNLYLDLGSLDREKDVELLTPGATLYFLEKGLYRVDVGNDG
                     RTEILVFEGLVETAAEEGSVLVKTGQGLSLADGRFEGRAKNFMTGATDAFDRWNGDRA
                     SLVRGTAESLGRLPVEIAEYESELDASGEWIDIEPFGFVWIPTGLSPGWRPYTWGRWT
                     WLPFSGWCWVPYEPWGWCTFHYGRWQWDPFYGWYWIPLPGWGAAWVNWWWNDYYCGWA
                     PMSYWGYPGIIYNNDYYGRGWDGDYPPESRALVVVRKSQLRDPNAGQAALPPGEIRSV
                     GRFRLSEAPPDFKPDPNAPLRLEPLDKRGAVLIRKDGGQGLSGNVPPESPRLIRPADR
                     PWNAGSEKELKTPEDSRPVRIAERPGTAPDSSRPAEPQERRIRKTYPSFDRFDRGDSG
                     KRESLTDLLRDKFYRVFQGEERKADSPSSKSGSVSGRRSSPRSSEGSASRSSGRSSSP
                     SRSSSPPKSSSGNSSSGSSGNVKKK"
     gene            complement(11531..12559)
                     /locus_tag="PKV64_07950"
     CDS             complement(11531..12559)
                     /locus_tag="PKV64_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HPN17049.1"
                     /translation="MTTGVLLDPRFNGHFMGPGHIECPERLEAIEALLKTELDFSPAR
                     IAARPASAAELARVHAPEYIDFLSRTRERAHTPLDGDTAASSLSYEAALLAAGGTIEA
                     ADFVLAGPGRNAFALVRPPGHHAERGTAKGFCLFNNVAVAAEHLIRERGLRRILIADW
                     DVHHGNGTQNAFYARDDVLFFSTHRSPFFPGGGGWDEIGDGDGRGYTVNAPLSAGRTD
                     GDFLFIYRNILGPIARAFTPEFILVSAGFDIYAGDPLGGMRVSLEGFAGLASELMDLA
                     QETCGGRLLLVLEGGYHIDGQARAVREVLRRMAGTRAAPDIRADISITTQKEITPVVD
                     VQRAFWPL"
     gene            complement(12564..14033)
                     /locus_tag="PKV64_07955"
     CDS             complement(12564..14033)
                     /locus_tag="PKV64_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPN17050.1"
                     /translation="MSLYLYIILAYLLVLTGMNFYRARKIKSQEDYQVAGRSLKWQVM
                     VFTLICTWIGSGTFISGAEFAAKAGISALWLAAGAWVGIVLIYFLAAKIHRFGQYTIG
                     DILEVRYGPAARLFGAIALILSFVSIVSYQFVAGGFILNVASDGKISAPLGTLIAAAF
                     VILFTAVGGMVAIAYTDLPNGIIIVLACLAAVPFVYAASGGIETARQALPPGYFAAVN
                     SQFGAHPVLKAVGYFLSTLFLLLGVQSMYQKFYSAKSARDARKAVIWWTLGTIFVESV
                     VVIIAVFAFSKLQGQIDLSVPKEGGKVVLMAARSLVPAPVGVLLLGAACAVVISTGMN
                     YLLSQSTTIMRDIYQRFVKKDAEARRIIALQKILVVVVGILAFLMATQLRSILETSMF
                     AYTIYGAAVTPALIAALVWKRATKAGGLASIIGGTVVCLFFFIMTRILPPDRVPDGDP
                     WGIPLIYPALIVSIGSLIVVSLLTPRPEPKIIEKLFPSP"
BASE COUNT         2701 a         4317 c         4564 g         2518 t
ORIGIN      
        1 ccctcctggg cctttttctt cttgcccggc ccctgtatat cacgattttc aggattcaga
       61 ccgaatccat caagaatcta accgtgctcg gcctcatcgg ggccggggcg gcgacggtgc
      121 tcctggccgt ttccttggga ttcatcttct tccgtgacaa ggccctcgcc ctgacagggg
      181 cggttctccg ggcccggatc attccggagc gcttccggtc gaaggtcctc gagctcctgc
      241 acgagttcat cgacgggttg aaattcttcc agtccctcaa gaccttgctg gcctatatcg
      301 gcctgtcctt cgtcgtttgg ctggggatcg ttttcttcta ctgggtcttt tttctggcct
      361 tcgacatccg gatccgtttc ttttttctct tcccttatgt ttttctgacc ggagtcgggg
      421 ccgcgatccc gaccccgggg atggtggggg gatatcacgc cttcagcaag cagggcatga
      481 tcgagctctt tgggatgaac gcaaatgaag ccggaggcct gaccgttgtc tttcacgcgg
      541 tccaatacat cgtcagctgc ctggttggat tgattttcct ctggatcgac gggctgaccc
      601 tgattcagat caagcggctc ggaaaggcga agaccccatg aaatgtccgt tttgcggcca
      661 cgaggaaagc aaggtcatcg attcgcggga gagcaaaaag gggatttcca tccggcgaag
      721 gcgcgagtgc atcgcctgcg gccgccggtt cacgacctac gagaagatcg aggaaatccc
      781 ctacatgatc gtcaagaagg acggctcgcg gcagctcttc gaccggcaga agctgttgcg
      841 cggcctcctc aaggcttgtg aaaaacggcc catccccgtg tccaagttcg aggaaatcgt
      901 cgaggaggtc gagtccaagc tccaagagcg cccggagaag gagatgaagg cctccgaaat
      961 cggcgagatg gtcatggcca ggcttaagga cctggacaag gtggcttacg tccggtttgc
     1021 ctcggtttat cgcgaattcc gggacgttct ggagttcaag caggagctgg agagccttct
     1081 gcgggaaaaa taggaagccc gccccgccga tttgacaaac gcccgccgcg gacgtatcta
     1141 tgaacgtcgc atgatcaagc gccttcatcc ggtcatccgg atccggacga gcgggggcgg
     1201 cgtctatccg gcggattggc aggggggctg gatgccgcgt ctcgatctga tcgagacgga
     1261 aaagacgctg atcgtggccg tcgaggtctc gggcttgcgg accgaggacc tccagatctc
     1321 cctgcagacg aaccggctgg agatcaaggg acggaagaag gaagcagccg ttcccgcggg
     1381 gacgcggtat ttccgcttgg agcgcgaata cgggccgttc cggcggacgc tggcccttcc
     1441 ccggtcggtc gtccccgatc gggcccgggc gactctcgaa aacggtgttt tgacgatcgc
     1501 ccttcccaag cggatcgaaa cgccgcggcg gcgacgcctt gtccgcatca agagaaacgt
     1561 ggagtgaatg gaatggctga aaaagatccc caatcggatc cggtcgacgg cctcgccgac
     1621 ggccaggatt ccaaaccgtc cataccggtc aagctgccga tccttctgct gcgggacatc
     1681 gtcgttttcc cttacatgat cgtcccgctg ttcgtcggcc gggaaaaatc caaggcggct
     1741 atcgacgagg ctctcgcttc ccaccggatg atccttctcc tgacccagag ggatgtggat
     1801 accgaagatc ccaagcagga cgaagtctac caaatgggga cggtcgccct gatcatgcgg
     1861 atgctgaagc tccccgacgg ccggatccgc atcctggccc agggattgac ccgggccgcg
     1921 gtccggacct acgacgagga gaacggccat ttcatcgccg aaatcgagcc gatcccggag
     1981 ccggaggacc aggaaaaatc gctggaaggg gaagccctcg tccgcaacgt ccggtcgggg
     2041 ctggaaaaag ccgcctcgct cggcaagagc attccgccgg aggttttgat catcgcctcg
     2101 aatgtggagg aacccggccg gctggctgac ctgaccgggg ccaacctcga gctcaaggtc
     2161 cccgaggccc agtcgatcct cgagatcgtc gatccgatcg cccgcctcaa aaaggtccac
     2221 gatgacttga ccaaggagat cgagatcctc gacgtccagt cgcggatctc gaccgaggcc
     2281 aagggcgaaa tggacaagca tcagcgccag tatttcctcc gccagcagat gaaggccatc
     2341 cagaaggagc tgggcgaggg caacgagctg caggaagaaa tccggagctt tcaggaaaag
     2401 ctgaaaaagc tcaaggtgac ggaagaagtc cggggggaaa tcgagaagaa tatcagccgg
     2461 ctgtcccaga tgcatcccga gtcggccgag acgacggtcg tccggaatta cctggattgg
     2521 atgttcgccc ttccttggag caagagcacc atcgataatc ttgacttgaa gaaggccaaa
     2581 gccatcctcg acgaggatca ctacggtctg gacaaggtca aggaacgaat catcgagtat
     2641 ttagccgtcc ggaagctgtc ccggcggatc aaggggccga tcctgtgctt tgtcggaccg
     2701 ccgggcgtcg gcaagacgtc cctcggcaaa tccatcgccc gggccctcgg ccgggaattc
     2761 cttcgcattt ccctgggcgg agtgcgggat gaagccgaga tccgcggcca ccggcggaca
     2821 tacgtcggag ccctgcccgg ccgaatcatc cagggcctcc ggagggccgg atcgaacaat
     2881 cccgtgttca tgatggacga ggtcgacaag atcggggccg attaccgggg cgatccctcc
     2941 tcggcgctcc tcgaggtctt ggacccggag caaaacaaca cgttccgcga tcactacctg
     3001 gccgtgccgt tcgacctgtc ccgggtgatg ttcatcacga cggccaacct tctggatccc
     3061 atccagcccg ccttccgcga ccggatggag atcatccagc tccccggcta caccgaggag
     3121 gaaaagatgg agatcgccgc ccggcacctg gtccctaagc agatcaagga gaacgccctc
     3181 tcggataaat acatccaggt gacgcccggg gcgatccgga cgatcatctc ccgctacacc
     3241 cgggaggcgg gcgtgcgtaa cctggagcgg gaaatcgcct cgatctgccg caaggtcgcc
     3301 cggaaggtcg ccgagaataa aaagacgctc aacaagatca cgtcccaaag cgtcgagtcc
     3361 tacctggggc cgccgaagat cttcaaggat gagctccgca agagcgaccg ggtgggcgtg
     3421 gccacgggcg tcgcctggac ggagacgggc ggggaagtcc tgttcgtcga ggccctgaag
     3481 atgaaaggca agggcggcct gatgctgacg ggcctgctgg gcgacgtcat gaaggaatcg
     3541 gcccaggcgg ccctcagcta tgcccgcgtc cacaccaagg agctgaaaat cgacaaccgg
     3601 attttcggga cgaacgattt ccacgtccat atcccggagg gggcgattcc caaggacggc
     3661 ccgtcggccg gcatcaccat cgccgccgcc ctcatttcct tgtgcacaaa ccgcgccgtc
     3721 cgctgggaca cggccatgtc gggcgagatc accctccagg gcaacgtcct gcccgtcggg
     3781 ggagtcaaag agaaagtctt ggccgcccag cgggccggcg tgaagcggat gatccttccg
     3841 tcgcccaacc ggaaggattt gatcgacatt cccaaggtgg tcaagaagga gatggagttc
     3901 atcttcgtcg aggacatccg ccaggtcctg gaggccgccc tcgtccggaa aacgggccgg
     3961 cctacttgaa gagaaacgac ccgacgaagg cgaattcggc cggcccggtc tgaacgaccg
     4021 gggccgtctc ggaaggccac tcgacgagga gggagcccat ctccgtccgg acggtgaccg
     4081 tccggtccgt gcgtcccgtg acgatcgcgg ccacggccgc cgcggccgcc ccgctgccgg
     4141 aagacagcgt ttccccgacg ccgcgctccc agaagacgac ctcgatctcg tcccgtcccc
     4201 ggacccggat gaactcgatg ttcgtccgcc gggggaaaaa gggatgcgat tcgatttccc
     4261 ggccgagctc atgccactcg aaccggcccg ggaagcggtc gatgaagaca tcgcagtgcg
     4321 gattcccgag agacacggcc gtgaccgggt aggtttcggt gttgatcacg agaggataat
     4381 cgaggatccg gtcgtggacc gttccgtcgt cgaaggggat atcggccggg gcgaaccgcg
     4441 gaatccccag ctcgatcttc atgagaaacc cgtccttggt ccggcggacg agggcgcagg
     4501 tccgttcgcc ggcggccgtc tggaagcgga cgacggggac gtcgatcttc tgttgggaat
     4561 agagataagc ggcggcgcac cggagaccgt tgccggagat ttccgcctcg gatccgtcgg
     4621 cgttgaaaat ccggaaccgg gcctcgcccc gggaccggtt cgtgaccgag acgaggagaa
     4681 tcccgtcggc cccgacgccg gtgtgccgct cgcagaggcg atgagccagg tcccccggct
     4741 cttcgaacag cgcgacttcg tcctggtcga cgatcaggaa atcgtttccc agggcgtgga
     4801 acttggtgaa tttcatggtt aatcggtgat gcgcagggtg acgatgaatt ccgagaattg
     4861 accgctgtcg tcctctctcc ggacggccag catgagggga tcgcccggtt tgagaggctt
     4921 gatcatggcg ttccactggt cgagcgtttc gattttcttc cggttggcct cgatgatgat
     4981 gtcgcctgga gcgaggccgc ggcggtcggc tggaccgccg ggagcgacgt cggtgacgag
     5041 gagtccgcga tcggtcttga gaccgtagcg gcgggccagg ggcggcgtca gggcttccac
     5101 cgacagcccg atgttcttgg gcgcggcccc ggccgccggc cgggactttt cctcgggcgc
     5161 gagctcgatg accttcgtgg tcacggtcat ctcttttcca tctcggatga accggatttc
     5221 gaccgggctg cccggcggaa tctccgagat cttgaaccgc agatcgttgc tgttttcgat
     5281 cggctggccg ttgacgccga tgatgacgtc ataccgcttg aggccggctt tttcggccgg
     5341 cgtgccgtcc tcgacgtcgt tgatgaccgc ccccttgcga tccttgaggt tcaaggcctt
     5401 ctgggtatcc tgatcgatcg ggcggatggc gatggaaacg cccagcctgc cccggacgac
     5461 ccggcccttg tccttcagct ggatgacgac tttcttggcc atggaggcgg ggatggcgaa
     5521 tccaatgccg atgttgccgc ccgaaggagt gagaatattg ctgttgatgc cgacgaccag
     5581 gcccttgagg ttgacgagcg ggccgccgga gttgccccgg ttgatggccg cgtccgtttg
     5641 gatgaaatcc tcataggtgc ggctgtcgcc gagccccagt tggcggccct tggcgctgac
     5701 gatgccggcc gtgacggtat gctccattcc gagggggttc ccgacggcca gaacccattc
     5761 cccgaccttc agggcggtcg aatcccccag ctcggcgaag ggaagatcct tgccctcgac
     5821 cttgatcaag gcgaggtcgg tgagaacgtc ggttccgatg atattcgcct cgtattcgtc
     5881 cccggcgacg gtgaagactg agatcttctt ggctttttcg acgatgtgat tgttggtcag
     5941 gatatatccg tcgggggaga tgaagaatcc cgttccctgg acgatggccc gcgattcctg
     6001 gggcgtctgg ctccggggcc gctgtcccgg cgagccgaag aagcgatccc aaaagtcgtc
     6061 gaagggcgtg acccgtcccg tcattcccgg ctcgacacgc tcggcgtcga tgcggacgac
     6121 ggtggggccg atcttctccg aaacggcgac gaaatcgagg tccggccgga tcgggggagt
     6181 ctcggcgaat aaattcgttc ccagcgaacg cgtgtcgccg gacaggaagc tccgggcttc
     6241 tgttttcttt tcgggcagga agacgaagac gtacccggcg gccaggacgc cgaggacgaa
     6301 cgccgccgcc gctatccatg cgcttctttt cgtcatcaga gatcctccat cggaattgga
     6361 ttccgctgtt gtaagaaatt ttaagatagc acaaacgttc ccttgaatca atttccggat
     6421 tctcccggaa cttcaatatt cataatcttt tatcgaacaa tcatcttcct ttttgaacga
     6481 tattcgccgt ccggccgttg cattttcgcg cttccccggc tatcatcctg aggatgaaaa
     6541 aaggccggct gatcgtcttc gagggcatcg atggggcggg gaagtccacc caagtccgaa
     6601 tgctcaagcg caagcttcgg gcccgcggcc gcgacgtcgc cgctttccgg gagccttccc
     6661 gaagccgctt cggccggacg ctccgggcga aagcgaagga agccggatcc ctgacgccgg
     6721 ccgaggagct cgatcttttc atcaaggacc gccgggagaa tgtcgcccgg aatatccgcc
     6781 ccgccctgag ggacggccgg gtcgtcctcc tggaccggta ctatttttcg acgatggcct
     6841 atcaaggagc caagggcctc gatccggaat ggatccggac cgtcaacgaa cgcttcgctc
     6901 ccaagcccga cctcgttttc atcctcgacg tgggcccgcc cgaggggctg gcccggatat
     6961 cgaggcgcgg gacgcgggac gccctgttcg agcgcgaatc ctatctgcgg aaggtccgcc
     7021 gcctcttccg gacgttccag ggcccgcggt tcgtccacct cgacggaaaa cgcgacaagc
     7081 gtgatttggg gcgggagatc ctggaccgga ccctggccgt ccttggggaa tagcgactca
     7141 tggccgcggc gcggaaagga ctcaaaatca tcttccggag gaacccccgg aaagcgggcg
     7201 cgggaagccg ccggaaaaga gcatggaagg tcgtcctctg ggcggcgttt gtcggcacgg
     7261 ccctgctttt cggagcggtt ttcgggacgt tcgtcgcggt ccgccggacg cttcccgatc
     7321 tctccggcct tgaatcctac gaaccggccc tttccaccaa ggtcctggcc gacgacggtc
     7381 aagtcgttcg ggaaatcggc gtggaaaaac ggacgattgt cacctacgag cagattccgg
     7441 ccgtcctgcg cgacgcgatc ctggcgaccg aagaccctca ttttttcaag caccgcgggg
     7501 tggacgttcg ggggattctc cgctcggtca aggagaacgc cctcaacgtc ttccgccgcc
     7561 gcaagctgca cggcgggagc acgatcaccc agcaggtcgc ccgcaagctg tttctccatc
     7621 ctctccaaac cctccagcgc aagttcgccg aatggtacgt ctccatccag atcgaaaaga
     7681 aatactccaa ggaccgcatt ttcgaaatgt actgcaacca gttcgagctc ggtccgggag
     7741 cggcgggggt cgaggcggct tcgcgtcttt atttcggcaa atccgtcggc gatttgatcc
     7801 tcgaggaagc ggccttgatc gccggcttgt accgcggccc aacgctttat tcgccctacc
     7861 ggaatccggc cctggccctc gaacggcgca atcatgtctt gacccggatg gcggaggagg
     7921 ggatgatcac cgccgcccgg gccgaagacg ggcggaaaac gccggtcgcc gtccttccgc
     7981 cggggcggga ggattatgat ttcggagcat atttcttcga ggaagtccgc cgggcgatcg
     8041 ccgaaaaata cggggaagag gcgctttact cgggcggcct gaaggtttcg acgacgctca
     8101 acgccgcctg gcagaaattc gccgacgaag ccgtccagaa cggtttgcgc gcctatgaca
     8161 agaggcacgg ctggcgccgg gacaaggcga atctcctggc cgatgaggcc tttaaagcct
     8221 ccgggaagaa gctcgaggag taccggctga ggtcatgggt cacgccccgc ctcgagcccg
     8281 gggacatcga ggaggccatc gtcctctccg tcggccggaa ggaagtccgg atcaaggtca
     8341 aggacgattt cgcccgtttg atcaatgagg gggcggaatg ggccgccccc ggcaaggccc
     8401 tggattccct cctcaagccc ggggatgtga tccaggtccg ggtgaaatcc cgggacgcgg
     8461 aaaaaaaaga aaccgtggcg accctggagc aggagccgct cgcccaggcg gccttggtgg
     8521 ctattgatcc ccgaacgggc cagatcaagg cgatggtcgg cggctcatcc ttccgccgga
     8581 cgcagctcaa ccgggcgacc cagacgtccc gacaggccgg atcggcgatg aagccgttca
     8641 tctatacggc cgccctggag cgcggcttca cggccgccag ccgcctcgtc gacgagccga
     8701 cggacttccc cgatccctgg tccgggacgg tttggacgcc ccgcaattac gaccggaaat
     8761 acaagggaat gatcaccttc cggacgggct tggaagagtc gcgcaacgtc atcacggcca
     8821 agatcctgga caacatctcc ccccagaccg gcgtggatta cgccaagcgc ttcggcttga
     8881 ccggaacact gtatccttat ctctccctgg ccttgggcac gttcgaggtc aatctcgtgg
     8941 aaatggtctc ggcctacggc gtcttcccca acaagggaat ccggatccgg ccgtttttcg
     9001 tcagccgggt cgaggaccgg gacggcaatc tcctggagga gcatgtcgtc gaggccgagg
     9061 aggtcgtctc gccccagacg gcgtttctga tgacccacct gatggagggc gtcgttcagc
     9121 gcggcacggc tgcggccgcg agcgtctttc tcaacgatac ggccttaggc ggtaaaaccg
     9181 gcacgacgga cgactgcacc gacgcctggt acatcggcta ctccccgtcg ctctgtgtcg
     9241 gggtttgggt gggcatggac gacaacacct ccctcggccg gaatgaaacc ggggccgtcg
     9301 ccgcccttcc gatctggatg acttttttcg gccgcgtcct cgaggagata aaggcgaagg
     9361 cgaaggatgc gtccgtcgag tattatcccg aagaattcga gaccccgccg aacatccact
     9421 tcatggccat cgaccgcaag accgggcttc tagccacccc gcagtgcaaa tggcggttca
     9481 tggaagcgtt tctcgaagga accgagccca cccggctgtg ttcgatgctg gatcacatct
     9541 cgacgctcga ttacggcggg acgtccgtgg cccgggaaga gcgctgacgg cttcggcggt
     9601 cgtgatccgg ccggctattt ctttttgacg ttccccgagg atcccgagct gctgttgcct
     9661 gaagatgatt tcgggggaga ggacgagcgg gacggggagg agctccggcc gctggagcgg
     9721 gacgccgagc cctcgctgga gcggggggag gatctgcgtc cggaaacaga tcccgatttc
     9781 gaggacggcg aatccgcctt ccgctcctcg ccttggaaaa cccggtagaa cttgtcccgc
     9841 aggaggtcgg tcagggattc gcgttttccg gagtcccctc gatcgaaccg atcgaaggac
     9901 ggatacgtct tgcggatccg gcgttcctgc ggttcggccg gccgtgagga gtcgggggcg
     9961 gttccgggcc gctcggcgat ccggacgggg cgggaatcct ccggcgtttt cagctctttc
    10021 tccgaacccg cgttccacgg ccggtcggcc ggccgaatca gccgggggct ttccggggga
    10081 acgttgccgg acaggccctg cccgccgtcc ttgcggatga ggacggcgcc ccgtttgtcg
    10141 aggggctcaa gccggagagg ggcattcgga tcgggcttga agtccggcgg cgcttcgctc
    10201 aggcggaagc ggccgaccga gcggatttcc cccggcggca gggccgcttg ccccgcgttg
    10261 gggtcccgga gctggctctt ccggacgacg accagggccc gtgattcggg cggatagtcg
    10321 ccgtcccacc cgcggccgta ataatcgtta ttgtagatga tgccgggata cccccagtag
    10381 ctcatgggag cccagccgca ataataatcg ttccaccacc agttgaccca agccgctccc
    10441 cagccgggga gagggatcca ataccagccg taaaacggat cccattgcca acggccgtaa
    10501 tggaaggtgc accaccccca cggctcgtac ggcacccagc accatccgga gaacggcagc
    10561 caggtccagc gtccccatgt atagggacgc cagccagggc tcaagccggt gggaatccag
    10621 acgaagccaa acggttcaat gtcgatccac tcgccggaag catccagctc cgattcgtat
    10681 tcggcgattt ccacggggag ccgtccgaga gattccgccg tcccgcggac gagggaggct
    10741 cggtcgccgt tccagcggtc gaaggcgtcg gtggcgccgg tcatgaaatt cttcgcccgg
    10801 ccttcgaatc ggccgtcggc caacgacagg ccttgtcccg tcttgaccag gacggatccc
    10861 tcctcggccg cggtctcgac cagcccctcg aagacgagga tttccgtccg tccgtcgttc
    10921 ccgacgtcga cccggtagag gcctttttcc aggaaataaa gcgtcgcccc gggcgtgaga
    10981 agctcgacgt ccttttcccg gtccaaggaa cctaaatcga gatagagatt ccccgcccag
    11041 tggcgaaggc gaagcaggga ggaatggtcc cgggggaggg tttgaacgtc gattttcgtg
    11101 taatgatcca agcgggcgta cttgatgtcg ccgaaggaga tctccgcccg gccctcgtcg
    11161 gttccgagcc ggtcgccctc gctgatcggg gtgttgatcg tcgcttcttc ggaggccagg
    11221 tcggcggccc gctgaaggaa aatccggccg tcgacaaagc tcaggcgagc gtaggagtac
    11281 cgcccgccgg ccgcttcccg gccggcggag tccggcggaa cggcatcctg acccgaagcc
    11341 caggtgaaga acaataagac ggcggcggcc gcgagcgctg tttttctcat cggatttctc
    11401 cttgcatcct cattatagca agaaacatac caggcgttcc cgcctgaaaa agcggatttt
    11461 cggagcccgg atcgtcctga gaaagagacg tcgtgtcctc ggatgaggac tccccctgcc
    11521 cgggaacggc tcaaagaggc cagaacgccc gctggacgtc gacgacggga gtgatttcct
    11581 tctgggtggt gatggagata tcggctcgaa tgtccggcgc ggcccgcgtc ccggccatcc
    11641 ggcggaggac ttcccggacg gcccgggcct ggccgtcgat atggtatccc ccctcgagaa
    11701 caagaagcag ccggccgccg caggtttcct gagccaggtc catcagctcc gaggccaggc
    11761 cggcgaagcc ctccaggctg accctcatcc cgccgagcgg gtcgccggcg taaatatcga
    11821 agccggccga aacgagaatg aactccggcg tgaacgcccg ggcgatcggg ccgaggatat
    11881 tccggtagat gaaaagaaaa tccccgtcgg tcctgcccgc cgagaggggc gcgttgacgg
    11941 tgtatcccct tccgtctccg tctccgattt catcccagcc gcctcctcca gggaaaaaag
    12001 gggaccgatg ggtcgaaaaa aagaggacgt cgtcgcgggc gtagaacgcg ttctgggtcc
    12061 cgttgccgtg gtgaacgtcc caatcggcga tgaggatccg gcggaggccc cgttcccgga
    12121 tgagatgttc agcggccacg gcgacgttgt tgaacaggca aaatcccttg gccgtccccc
    12181 gctcggcgtg atggccgggc ggtcggacga gagcgaaggc gttccgtccc ggcccggcga
    12241 ggacaaaatc ggcggcttcg atggttccgc cggcggccag gagggcggcc tcgtaggaca
    12301 gcgacgaggc cgcggtatcg ccgtcgagcg gcgtgtgggc ccgctcccga gtccgggaga
    12361 ggaaatcgat gtactcggga gcgtggacgc gggcgagctc ggcggccgag gccgggcggg
    12421 ccgcgatccg ggccggggaa aaatccagct ctgttttaag gagggcctcg atcgcctcga
    12481 gccgctccgg gcactcgatg tgccccggtc ccatgaaatg gccgttgaaa cggggatcga
    12541 gaagaacgcc ggtggtcatg agctcatggg gacgggaaga gcttctcgat gatcttgggc
    12601 tccggccggg gcgtcaggag ggacacgacg atcaggctgc cgatcgagac gatcaaggcc
    12661 gggtagatga gggggatgcc ccagggatct ccgtccggga cccggtcggg gggcaggatc
    12721 cgggtcatga tgaagaagaa gaggcagacg accgttcctc cgatgatcga ggccagcccc
    12781 ccggctttgg tcgcccgttt ccagaccagg gcggcgatga gggcgggggt gacggccgcc
    12841 ccgtagatcg tgtaggcgaa catgctggtc tccaggatgc tccgcagctg ggtggccatc
    12901 aggaaggcca gaatgcccac gacgacgacc aggatttttt ggagagcgat gatccggcgg
    12961 gcctcggcgt cctttttcac aaagcgctgg tagatgtcgc gcatgatggt cgttgattgg
    13021 gacaggaggt aattcatgcc cgtcgagatg accacggcgc aggccgcgcc caggagaagc
    13081 acgccgacgg gggcgggaac caggctccgg gcggccatca agacgacctt cccgccctcc
    13141 ttgggaacgc tgaggtcgat ctgtccctgg agcttgctga aggcgaagac ggcgatgatc
    13201 acgacgacgg attcgacgaa gatcgttccc agggtccacc agatgacggc tttgcgggcg
    13261 tcccgagccg atttggcgct gtagaacttc tggtacatgc tctgaacgcc gagcaggaga
    13321 aagagggtcg agaggaaata gccgacggcc ttgaggaccg ggtgggctcc gaactggctg
    13381 ttgaccgcgg cgaaataccc cggcggaagg gcttggcggg cggtttcgat ccctccggag
    13441 gcggcgtaga cgaagggaac ggccgccagg caggcgagga cgatgatgat cccgttgggc
    13501 aggtcggtgt aggcgatggc caccatgcct ccgaccgcgg tgaagaggat gacgaaggcg
    13561 gccgcgatca gcgtccccaa gggcgcggaa atctttccgt cggaggcgac gttcaagatg
    13621 aatccgccgg cgacgaattg ataggagacg atcgagacga acgaaaggat gagggcgatg
    13681 gccccgaaca accgggcggc cgggccgtag cggacctcga ggatatcgcc gatcgtgtac
    13741 tgtccgaacc gatggatttt cgcggctaaa aaataaatca ggacgatgcc gacccacgcc
    13801 ccggccgcga gccagagggc cgagattcca gccttggccg cgaattcggc cccgctgatg
    13861 aacgtccccg agccgatcca ggtgcagatc agggtgaaaa ccatgacctg ccatttcaag
    13921 ctccgtccgg cgacctggta gtcctcctgg ctcttgattt tccgggcccg gtagaaattc
    13981 atcccggtga ggacgagcag ataggcgagg atgatgtaga gataaagcga catgccgcct
    14041 ccttgaaaag ccgtctccgc ccgcccgcgt cattcccccg gcccggcgaa aaaaggccgg
//
LOCUS       DAOOXH010000062        14024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_4405_length_14024_cov_7.872933, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000062 DAOOXH010000000
VERSION     DAOOXH010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 14024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14024
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4405_length_14024_cov_7.872933"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..246)
                     /locus_tag="PKV64_07960"
                     /pseudo
     CDS             complement(1..246)
                     /locus_tag="PKV64_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507736.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(552..1550)
                     /locus_tag="PKV64_07965"
     CDS             complement(552..1550)
                     /locus_tag="PKV64_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HPN17051.1"
                     /translation="MGLLEVLFGRRFPFKFVYHEEYWMLNTGKHIFPVRKYRMVHERL
                     AALGARRENFLKPEPASFEDLQLVHSLKYLRKLQTGSLSKDEIQALELPFSAELISFF
                     RLNVGGTVLAAERALDDGLCIHLGGGFHHAFADHGEGFCLLNDTAVAVEKLQREGRVG
                     RVMIVDCDVHQGNGTAAIFEKREDVFTFSIHQMDIYPSEKMTGTLDVGLWSGDGDRAY
                     LEAVRRHFPKIYEEFPPDIVFYLAGADPLAGDQLGGLAVTKAGLLSRDRIIMEGALLR
                     KIPLVILLAGGYSRDPAETTDAHVNTVRTAAVMKRRAARLIAQREAEKLDRPVYRP"
     gene            1841..3961
                     /locus_tag="PKV64_07970"
     CDS             1841..3961
                     /locus_tag="PKV64_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPN17052.1"
                     /translation="MSDFRIDRHPILTPSPAEKVAMTWKGRPISARAGETIAAALFAN
                     GERVFSYHPKDRAPQGIFCANGQCAQCLVLADGLPVKACMELVRPGLKLEPVDGLPVL
                     PPVSSPVRLSPIEEISVPVLIIGGGPAGLSAAIEFGKRGVSALLIDDKHRLGGKLVLQ
                     THRFFGSYNAVFAGTRGIDIAAKLENELRTYPSVRIWLQSTALAVYSDKKVGILRDGK
                     RYVQVNPEVLLVASGAREKSLAFPGNTLPGVYGAGAFQTLVNRDLVRSAGRLFIVGGG
                     NVGLIAGYHALQAGIPVVGLVEALPECSGYKVHKDKLRRFGVPIYTSHTVVSANGTDG
                     VQSVTIAALDKDWKAVPGSEKSFACDTLLIAIGLDPVDEFYDKAREFGLPVYAAGDAE
                     EIAEASAAIFSGKIRGLEILKAMGRNDGGISPDWYATADILKSRPGKTVPEAPPEGET
                     GVFPVLHCVQEIPCDPCTTVCPRDLIHIHSGDIRGLPAFEALDPARACTGCEKCVTIC
                     PGLAIVLVDYRKDPASPTVTIPYEFLKDGIDVGDRVRILDTEAADLGEVEVVGVRSIK
                     ANDRTVLVKVRAPAAVAKRIAGIRVQDPAAGKPLDRVVERTADDVIVCRCERVAAGEI
                     RALIREGFRDISAIKAVSRAGMGACGAKTCTALIHRLFREEGVPEKEIIDQSKRPLFM
                     EVPLGTFAGSEPGPVREGGDRGRY"
     gene            3948..5111
                     /locus_tag="PKV64_07975"
     CDS             3948..5111
                     /locus_tag="PKV64_07975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPN17053.1"
                     /translation="MAAIKSSADVIIIGSGSVGTPAALAMAESGLRVLVIDRMPSVGQ
                     GSNKGAIGGIRATHSDPAKIRLCLRSLEIFSTWRDRRGDDIEWRKCGYMFVAYRRQEE
                     KALKELLVTQKAFGLNIDWLDRKAMLEIVPDLNPEGLIGGTYSPDDGSASPLLSAHAF
                     WRAAKKAGAMFRFGETVTGLIMENGAVRGVRTNKGEYAAEIVVNAAGGWADEVGRMAG
                     LDLPVKPDAHEGAVTEPVAPFLHPMIVDIRPTDSSVNYYFYQHATGQIMFCITPNPSI
                     WGLEKRETSTFLPMVAHRMVNLIPRLQNLRVRRTWRNVYPMTPDGFPLVGWAREVKGL
                     LLAEGMCGQGFMLGPGLGELLARIVRNDLPAKDREILALLSPYRTFEGREKLQ"
     gene            5214..5813
                     /locus_tag="PKV64_07980"
     CDS             5214..5813
                     /locus_tag="PKV64_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17054.1"
                     /translation="MKRLPAVLAPALIAIASAGCVMAPRINLDRFGTIGFIGFDTRAE
                     GNIDEYANQVFLEVLLRSQPGTRIIDLGPAATVLRGSRSPVPTPEILADLRSRYNVDA
                     VLTGILDVSKARPRVELGAILLGSIRASVDMDAFMSARLMDTHDGTTFWMDSARVRMD
                     IADLSVLKNGQVMFDARDPERAYGRLIRELVLRTTRDFR"
     gene            5810..6184
                     /locus_tag="PKV64_07985"
     CDS             5810..6184
                     /locus_tag="PKV64_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015945517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPN17055.1"
                     /translation="MTFTFAHNNVNVFDLDRSLAFYKEALGLAEVRRIAAKDGSFLIV
                     YLGDGRSAHRLELTWLRDRTEKYDLGDNEIHLAFRTDDYDGAFRKHKEMGVVCYENPT
                     MGIYFIEDPDGYWIEILPQSRG"
     gene            complement(6202..7527)
                     /locus_tag="PKV64_07990"
     CDS             complement(6202..7527)
                     /locus_tag="PKV64_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HPN17056.1"
                     /translation="MKNARIRLFIFLAVVTAAFFAGPRPASGRDIPAILGLKDRAAVR
                     DRWLGLRLETVLPEVMRREKIDMWLVICAEDNEDPVYLTLVPADSYSARRTTMLVFFD
                     RGKNGIERLTVGRYGIGKLYPASWDPEKIDQWSRLAEIVKERNPSRIGINESDVFAFA
                     DGLSASHKARLVRALGPDFSRRLVSAERLAVGWLERRTPDELDVYDHIAAVAHAIIAE
                     VFSRDAVTPGVTTVSDVVWRLRERVSSLGLETWFQPSVEIQRPGESLDGEADVIHRGD
                     LLHCDFGIRYLGLCTDTQQHAYVLRPGEEDAPAGLKAALAQGNLLQDILLEEFKEGRA
                     GNEILASALRKAKAAGLRPSIYTHPLGYHGHAAGPTIGLWDKQNGVPGDGDYPLFADT
                     VYSIELNITAAVPEWGGTDVRIALEQDAVFGRTGSRFLDVRQTAFHLIR"
     gene            complement(7574..10363)
                     /gene="ppdK"
                     /locus_tag="PKV64_07995"
     CDS             complement(7574..10363)
                     /gene="ppdK"
                     /locus_tag="PKV64_07995"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPN17057.1"
                     /translation="MSRKYVYFFGAGGAEGDMKMKNILGGKGANLAEMAGLGLPVPPG
                     FTVSTEVCDLFYKNRKRIPADVRAQIDKNLKKLEKAAGQTLGDSKNPLLVSIRSGARA
                     SMPGMMDTILNLGLNEKTVAALIAKTGNPRFAYDCYRRFIQMYGDVVLGLKPVRKEDV
                     DPFEKIIEEKKAERGLTADMDLTADDLKDLCVRFKAAILKNTGLEFPEDPREQLWGAI
                     GAVFDSWMNDRAIAYRKMYDIPEAWGTAVNVQLMVFGNMGPDSGTGVAFTRDAATGAN
                     VFYGEYLMNAQGEDVVAGIRTPLPIVMLKKDDAKSYAQLEKFRHVLEKHYADMMDIEF
                     TIQQGKLYMLQCRVGKRTAFAAIKIAVDMVGEKLISEKEALSRIEPDQLNQLLRPIFD
                     PAEKTAAIKAGRLLTKGLNAGPGAASGRIVFNSVDAEAWAGRGEKVILTRIETSPEDI
                     KGMNAAEGILTARGGMTSHAALVARQMGRVCVAGCGELEIDYAAQTVTVKGRKLREGD
                     WISIDGTTGEVLEGRVQTKPSEVLQVLIQKSLNPKDAPVYQTFAKVMSWADKVRILKI
                     RTNADQPDQAANAVAFGAEGIGLCRTEHMFFGEGKIEPMREMILADTIAERKAALARL
                     LPLQRRDFAGIFEVMKGLPVTIRTIDPPLHEFLPQAEKDQRALAAEMGIPYEKIKTRV
                     DSLHEFNPMLGFRGCRLGVVYPEITEMQARAIFEAAADTKKKGIPVEPEVMIPLVGNV
                     KELADQEGVVRDAAEAVMKEKAVKFKYHVGTMIEIPRGALTADEIASVAEFFSFGTND
                     LTQTTLGVSRDDAGRFLIPYVEKGIYAKDPFEVIDQDGVGQLMRIAVEKGRAARPGIK
                     LGICGEHGGEPSSVEFCHQIGLNYVSCSPFRVPIARLAAARAALKGAPEGKASAGVKK
                     PASTAKKAIKTSRRR"
     gene            complement(10456..11655)
                     /locus_tag="PKV64_08000"
     CDS             complement(10456..11655)
                     /locus_tag="PKV64_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPN17058.1"
                     /translation="MDISRRGQTVQASPIRKFKPYADAAIADGVKIFFLNIGDPDIPT
                     PKPILDAVRSFNDPILGYGPAQGFLELRQAIAGYFKDYGISLKADNVLITLGGSEAIL
                     MAFATVADPGDEIIIPEPFYTNYNGYASLAALKVVPLTLKVEDGFRLPPAEEFEAKIT
                     PRTRAIVLCSPNNPTGTVYTAEELGRVVKLAVKHNLFIIGDEVYKEFVYNGRRHISLL
                     EFEEARDRVIVVDSISKRFSCCGARVGAAITRNDQVYAAMLKFAQARLCPPTIEQRAA
                     IAAYKMGMGYFEPVRREYERRRDVLFEGLSAIPGVVVRKPEGAFYMSVRVPVRDTEKF
                     VIWMLTDFRLDGQTVMVAPESGFYGTPGKGKDEIRAAYVLNEDDLRRALVVFRAGLEK
                     YKATVEK"
     gene            11793..13955
                     /locus_tag="PKV64_08005"
     CDS             11793..13955
                     /locus_tag="PKV64_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPN17059.1"
                     /translation="MNLAPDAVLGHYRLAEKIGEGGMGVVWKARDLRLDRDVAVKFLS
                     EGPSLSPERRSLLEREARAVAALRHPNIVTIYSIEEFEDALFYTMELVEGTPLTKLIV
                     PGGIEFDRLLAVAFPLTEAIAAAHDRGIIHRDLKPGNVMIEPDGKLKILDFGLARVLE
                     PVLPPLTAGGEADTETAETGITGTLCYMSPEQIRGEAVDHRTDLFSFGVVLFELATGI
                     LPFSGNTAADLISSILRDEPRSAVKANPRLPASIDRIFGGLLEKDARSRIASARSLRD
                     SLEALRRESAGRQGEEIPSIAVLPPVDMSPEKDQAYFCEGIAEEIIIALGRVKQLRVA
                     SRSASFQFAGGSVDPREIGRRLNVGTLLEGSVRKDGSRLRISAQLLDTLHGFQLWSEI
                     FDREHGDIFAIQEEIARNVVRALQVTLSPREKGALGKISTRHVQAYDYYLRGRGFYNR
                     YTKQDIEFAFQLFTRATELDDSFASAFAGLADCWSYIFLYSERKESVRRQAEEAARRA
                     VELDAESGQAQASYALALSLGGRGEEAVDHFEKAVALEPGLFEGWYYYARHSFAQGEY
                     EKAIRLYEQACRVRPDDYQSPLLMAQIYEEVGRPEDARKSRLRGIELAESRLELHPDD
                     SRALYMGANGLVALGEKERGLEWARRARALAPDDSMLLYNLGCIYSLAGDYEEAMSCL
                     EKSIGVGRMTQKAWYEHDSNLDPLRGLPRFKELLDSLE"
BASE COUNT         2523 a         4607 c         4307 g         2587 t
ORIGIN      
        1 ggtgggcttc cgagacgagg cgaaagagct ctccatcgcc gtcgaagctc cacggccgcc
       61 ccatcttaac gatttcaaga cgcggttcgt cgtgccggta aaggatctcg tcggcgaccc
      121 ggccgtccag gccgcgatac acgagagtca aagcgtccga tccgtgccag gctacgctga
      181 caacggtaac cggagtgtcg ggatgccgcg gacagtcgcg ttcggttgaa gatcttcgag
      241 agtcaatcgg attcccctaa ccgtaataga agcccacctt aattgtcacg gcggggcaaa
      301 tccttgaccc gctcttaata agtttatcac ctgcatagtg agaaatcgag ggtttgccat
      361 ccattctata ggtcagctca tcgcctggtt atccggcatc tcaataacgc ttcgctatcc
      421 tcgcacagga tgtttcactt ttattccctt gagagatggc tccctatacg acgtgccaag
      481 atcgcccata tgttgcaccg agaaaacgtc cctcgacaaa ataccgagat cgcctgcttc
      541 atcattcgcg gctaaggccg gtaaacgggg cggtcgagct tttccgcctc gcgctgggcg
      601 atcaggcggg ccgcccggcg tttcatgacg gccgccgtcc ggacggtgtt gacatgggca
      661 tcggtcgtct cggccggatc ccgggagtag cctccggcca gcagaatcac gagggggatt
      721 ttccgcagaa gagccccctc catgatgatc cggtcgcggc tcagcaaccc ggctttcgtc
      781 acggccaggc cgcccagctg atcgccggcc agcggatcag ccccggccag gtagaaaacg
      841 atgtccggcg ggaattcctc gtagatcttc gggaagtggc gccgcaccgc ttccaaatag
      901 gcccggtctc cgtctcccga ccacagcccg acatccaggg tcccggtcat tttttccgaa
      961 gggtagatgt ccatttgatg gatggagaac gtgaaaacgt cctcccgctt ttcgaagatc
     1021 gcggccgtcc cgttgccctg gtggacgtcg cagtcgacga tcatcacccg gccgacccgg
     1081 ccctcccgct ggagcttctc aacggcgacc gccgtgtcgt tgagaaggca gaatccctcg
     1141 ccgtggtcgg cgaaagcatg atggaatcct ccgccgagat ggatgcaaag cccgtcgtcg
     1201 agcgcccgct cggccgccag aaccgtgccg ccgacgttca accggaaaaa ggagatcagc
     1261 tcggcggaaa acggcaattc cagcgcctgg atctcgtcct tcgacaggct tccggtctga
     1321 agcttgcgga gatatttcag cgaatgaacg agctgaagat cctcgaagga agccggctcg
     1381 ggcttgagga agttttccct tcgggcgccc aaggcggcga gccgctcatg gaccatccgg
     1441 tatttcctca ccgggaagat atgtttcccg gtattcagca tccagtattc ctcgtggtac
     1501 acgaacttga agggaaaacg gcggccgaaa agaacttcaa gaaggcccat ggcggaaggc
     1561 aaagcatatc ataggggttc cccctgagag aaaccctttt ctccccgctt tttcggatag
     1621 gccatccctc tcgactcgcg gatacaagga cggctttcgg cgaaatcatg gacgaacgcg
     1681 tcagccccga ggcacaatcg actgaaatga cctgatattt tttctttggt actccgctct
     1741 ttctttttaa cgccggcgag agccccttag atgtctgaaa tgtgatataa taaacactaa
     1801 ttccgcctga atctggcgcc agggagatta aggagataac gtgagcgatt ttcgcatcga
     1861 tcgtcacccc atcctcactc caagccccgc ggaaaaagtc gccatgacct ggaaaggccg
     1921 tccaatttcc gcccgggccg gagaaaccat cgccgccgcc cttttcgcca acggcgagcg
     1981 cgtcttcagc taccatccca aggatcgggc tccgcagggc attttttgcg ccaacggcca
     2041 gtgcgcccag tgcctcgtcc tggccgacgg cctgccggtc aaggcttgca tggagctggt
     2101 ccgtcccggc ttgaagctcg aaccggtgga cgggcttccc gtcctcccgc ctgtttcaag
     2161 cccggtccgc ctttctccca tcgaggaaat ctccgtcccc gttctcatca tcggcggcgg
     2221 cccggccggc ttgtcggccg cgatcgaatt cggcaagcgc ggcgtttccg ctcttctcat
     2281 cgacgacaag caccgcctcg gcggcaagct tgtcctccag acccaccggt tcttcggctc
     2341 ctataacgca gtgttcgccg gaacccgcgg catcgacatc gccgccaagc tcgaaaacga
     2401 gctccggact tatcccagcg tccggatctg gcttcagagc accgctctgg ccgtctacag
     2461 cgacaagaaa gtcggcatcc tcagggatgg gaagcgctac gtccaggtga atccggaagt
     2521 ccttctcgtc gcctcgggag cccgggaaaa atccctggcc ttccccggca acacccttcc
     2581 cggcgtttac ggcgccggcg ccttccagac gctcgtcaac cgggacctgg tccgctccgc
     2641 cggccgcctg ttcatcgtcg gcggcgggaa cgtcggcctg atcgccggat accacgccct
     2701 gcaggccggc atccccgtcg tcgggctggt cgaagccctc cctgaatgca gcggctacaa
     2761 ggtccacaag gacaagctcc ggcgcttcgg cgtcccgatt tacacctccc acaccgtcgt
     2821 gagcgccaac gggacggacg gcgtccagtc cgtgacgatc gccgccctcg acaaggattg
     2881 gaaggccgtt cccggctcgg agaaatcctt cgcctgcgac acgcttctga tcgccatcgg
     2941 cctcgacccg gtcgatgagt tctacgacaa ggcccgggag ttcggcctgc ccgtctacgc
     3001 ggccggcgac gccgaggaga tcgccgaggc ttcggccgcg attttctcgg gaaaaatccg
     3061 cggcctggaa atcctcaagg ccatgggccg gaacgacggc gggatctccc cggactggta
     3121 cgcaacggcc gatatcttga agtcccggcc ggggaaaacc gttcccgagg ctccgcccga
     3181 aggggagacg ggcgtttttc cggtccttca ctgcgtccag gaaattccct gcgatccgtg
     3241 cacgacggtc tgtccgcgcg acttgatcca catccattcg ggcgacatcc gcggcctgcc
     3301 ggcctttgaa gccctcgacc cggcccgggc ctgcaccggc tgcgaaaaat gcgtcacgat
     3361 ctgtcccggc ctggccatcg ttctcgtcga ttaccgcaag gatcccgcct ctccgaccgt
     3421 cacgatcccg tatgaatttc tcaaggatgg gatcgacgtc ggcgaccggg tccgcatcct
     3481 ggacaccgag gccgccgacc tgggggaggt cgaggtcgtc ggcgtccgtt cgatcaaagc
     3541 caacgaccga acggttctgg tcaaggtccg ggctccggcg gccgtcgcca agaggatcgc
     3601 cgggatccgc gtccaggatc cagccgccgg caagcccctg gaccgcgtcg tcgagcggac
     3661 ggccgacgac gtcatcgtct gccgctgcga gcgggttgcg gccggagaga tccgggccct
     3721 catccgggag ggattccgtg acatcagcgc catcaaggcg gtgagccggg cggggatggg
     3781 cgcctgcggg gccaagactt gcacggctct catccaccgc ttattccggg aggagggcgt
     3841 tccggaaaag gagatcatcg accagtcgaa gcggccgctg ttcatggagg tcccgctggg
     3901 aaccttcgcc ggcagcgagc cggggccggt ccgggaagga ggcgatcgtg gccgctatta
     3961 agtcctccgc ggacgtcatc atcatcggct cgggcagcgt cggaacgccg gccgccctgg
     4021 ccatggccga aagcggcctc cgcgtcctcg tcatcgaccg gatgcccagc gtcggccaag
     4081 gatcgaacaa gggggccatc ggcggaatcc gggcgaccca ctccgacccg gccaaaatcc
     4141 gcctctgcct gcgctcgctt gagattttct cgacctggcg ggatcgccgc ggcgacgaca
     4201 tcgaatggcg caagtgcgga tacatgttcg tcgcctaccg ccgccaggag gaaaaggctc
     4261 tcaaggagct tctggtcacc cagaaggcgt tcggcctgaa catcgattgg ctcgaccgga
     4321 aggcgatgct tgaaatcgtc cccgatctca atcccgaggg cctgatcggc ggaacgtatt
     4381 cgcccgacga cgggagcgcc tctcccctcc tttcggctca tgccttctgg cgggcggcaa
     4441 aaaaagcggg ggcgatgttc cgcttcggcg aaaccgtcac cggcctgatt atggaaaatg
     4501 gagccgtccg cggagtccgg acgaacaagg gcgaatatgc cgccgagatc gtggtcaacg
     4561 cggccggcgg ctgggcggac gaggtcgggc gcatggccgg tctcgacctt ccggtcaaac
     4621 cagacgccca cgagggagcc gtgaccgagc cggtcgcccc gttcctccat ccgatgatcg
     4681 tcgacatccg cccgaccgac agctcggtca attattattt ctaccagcac gccaccggcc
     4741 agatcatgtt ctgcatcacc ccgaatccca gcatctgggg cctggaaaag cgcgaaacca
     4801 gcacgttcct gcccatggtc gcccaccgga tggtcaatct gatcccccgg cttcagaacc
     4861 tgcgcgtccg gcggacatgg cgcaacgtct acccgatgac gcccgacggc tttcccctcg
     4921 tcggctgggc ccgggaagtc aagggactgc tgctggcgga ggggatgtgc gggcagggat
     4981 tcatgctcgg tcccggcctg ggagagcttc tggcccgcat cgtccgcaac gatctgccgg
     5041 ccaaggaccg ggaaatcctg gccctgctct cgccctaccg cacgttcgaa ggccgggaaa
     5101 aactgcaata accgtccggt tccgctgtgt tctcatccgg cccgcgccgg gacgttgccg
     5161 aaaccgcctc ccggctttat aatgggcgta ctttccagaa aggagagcgc cggatgaaac
     5221 gacttcccgc cgtcctcgcc ccggccctta tcgcgatcgc ctcggccgga tgcgtcatgg
     5281 ccccgcggat caacctcgat cgcttcggaa ccatcggttt catcggcttc gacacccggg
     5341 ccgagggaaa catcgacgag tacgccaacc aggtttttct cgaagtcctg ctccggtccc
     5401 agcccggaac caggatcatc gatctcggcc cggcggcgac ggttttgcgc ggatcgaggt
     5461 cgcccgttcc cacgccggaa atcctggccg atctccggag ccgctacaac gtcgatgccg
     5521 tgctgaccgg gatcctcgac gtctccaagg cccggccgcg ggtggaactg ggagccattc
     5581 ttctcggttc gatccgggct tccgtcgaca tggacgcctt catgtccgcc cggctgatgg
     5641 acacccatga cgggacgacg ttctggatgg actcggcccg ggtccggatg gacatcgccg
     5701 atctcagcgt cctgaaaaac gggcaggtga tgttcgacgc ccgggatccc gagcgggctt
     5761 acggccgcct gatccgcgag ctcgtcctcc ggacgacccg tgatttccga tgaccttcac
     5821 cttcgctcac aacaacgtca acgttttcga cttggaccgg agcctggcct tctacaagga
     5881 agccctcggg cttgcggaag tccgccggat cgccgccaag gacgggagct ttctcatcgt
     5941 ctatctcggc gacggccgga gcgcccaccg ccttgagttg acctggcttc gggaccggac
     6001 ggaaaaatac gacctgggag acaacgaaat ccacctggcc ttccggacgg acgactacga
     6061 cggcgcgttc cggaagcaca aggagatggg cgtcgtctgc tacgaaaatc cgacgatggg
     6121 gatttatttc atcgaggacc cggacggcta ctggatcgag atcctgccgc aaagccgggg
     6181 ctgagctccc ggcccgcctc gtcaccggat caggtgaaag gcggtctgcc ggacatccag
     6241 gaagcgcgat cccgtccggc cgaagacggc gtcctgctcg agggcgatcc ggacgtccgt
     6301 cccgccccat tcggggaccg cggccgtgat gttgagttcg atcgaataaa ccgtgtcggc
     6361 gaacaggggg taatcgccgt ccccgggaac gccgttctgc ttgtcccaca gcccgatcgt
     6421 cgggccggcg gcgtggccgt gatacccgag gggatgagta taaatgctcg gccggagtcc
     6481 ggccgccttg gctttccgga gagccgaagc gaggatctcg tttcccgcgc ggccttcctt
     6541 gaattcctca agcaggatgt cctggaggag atttccttga gccaaagccg ctttcaggcc
     6601 ggccggagcg tcctcctccc ccggcctgag gacataggcg tgctgctgcg tgtcggtgca
     6661 caaaccgaga taccggatgc cgaaatcgca atgaagcaag tcgccccgat ggatgacatc
     6721 ggcctcgccg tccagggact cccccggccg ctggatctcg acggacggct ggaaccatgt
     6781 ctcgagaccg agggacgaga cccgctctcg gagcctccag acgacgtctg aaaccgtcgt
     6841 cacgcccggc gtgaccgcgt cccgggaaaa gacttcggcg atgatcgcgt gggcgacggc
     6901 ggcgatgtgg tcgtagacgt ccagctcgtc gggcgtccgg cgctcgagcc agccgacggc
     6961 caagcgctcg gcggagacga gccgcctcga aaaatcgggt ccgagcgccc ggacgagccg
     7021 ggccttgtgc gaggccgaga gcccgtcggc gaaggcgaag acgtcggatt cgttgattcc
     7081 gatccgggac ggattccgct ccttgacgat ttcggccagc cggctccact gatcgatctt
     7141 ctccggatcc caggaggccg ggtacagctt gccgatcccg tagcgcccga ccgtcaagcg
     7201 ctcgattccg ttcttgcccc ggtcgaaaaa gacgagcatg gtcgtccggc gggcggagta
     7261 ggagtcggcc ggaaccaggg tcagataaac cggatcttca ttgtcctcgg cacagatcac
     7321 gagccacatg tcgatctttt cccggcgcat gacctcggga agaacggttt ccaggcggag
     7381 gccgagccac cggtcgcgga ccgcggcccg gtccttcaag ccgagaatcg cggggatgtc
     7441 ccggccggac gccgggcgag gcccggcgaa aaaggcagcc gtaacgacgg ccagaaaaat
     7501 aaaaagacga atccgcgcgt ttttcacgca acctcctccc ggacgcggcc gtccggccaa
     7561 gggctccgac cgctcagcgc cgtcgggatg tttttatcgc ctttttcgca gttgaagccg
     7621 gcttcttgac tccggcggag gcctttcctt ccggagcgcc cttcaaggcc gcccgggccg
     7681 ccgccaagcg ggcgatcggc acccggaacg gcgagcagct cacgtaattc agcccgatct
     7741 ggtggcagaa ctcgacgctc gaaggctcgc cgccgtgctc gccgcagatg ccgagcttga
     7801 tcccggggcg ggccgcccgc cctttctcca cggcgatccg catcagctgt cccacgccgt
     7861 cctggtcgat gacctcgaac gggtccttgg cgtagatgcc cttttcgacg taagggatca
     7921 ggaaacggcc ggcgtcgtcg cggctgactc ccagggtcgt ttgggtcagg tcgttcgtcc
     7981 cgaagctgaa gaattcggcc accgaggcga tttcgtccgc ggtcagggct ccgcgcggga
     8041 tctcgatcat cgtccccaca tgatatttga acttgaccgc tttttccttc atcacggcct
     8101 cggccgcgtc ccggacgacc ccttcctggt cagccagctc cttgacgttg ccgaccaggg
     8161 ggatcatcac ctcgggctcg acggggatgc cctttttctt ggtgtcggcg gccgcttcga
     8221 atatcgcccg ggcctgcatc tcggtgatct ccggatagac aacgccgagg cggcatccgc
     8281 ggaatcccag catcggattg aattcgtgga gcgaatcgac ccgggtcttg atcttctcat
     8341 agggaatgcc catctccgcg gccagggccc gctggtcttt ttcggcttgg ggcagaaatt
     8401 cgtggagcgg cgggtcgatc gtccggatgg tcaccgggag gcccttcatg acctcgaaaa
     8461 tgccggcgaa atcccgccgt tgaaggggaa gaagacgggc cagggcggcc ttccgctcgg
     8521 cgatcgtgtc ggccaggatc atctcccgca tcggctcgat ctttccttcg ccgaagaaca
     8581 tatgctcggt ccggcagaga ccgatgcctt ccgccccaaa ggccacggca ttggccgctt
     8641 ggtcgggctg gtcggcgttg gtccggatct tgaggatccg gaccttgtcg gcccaggaca
     8701 tcaccttggc gaaggtctgg tagacggggg cgtccttggg attgagggat ttctggatca
     8761 ggacctgaag aacctccgaa ggcttggttt ggacccggcc ctcgaggacc tcgccggtcg
     8821 tgccgtcgat cgagatccag tcgccttcgc ggagcttccg gcccttgacg gtgaccgtct
     8881 gggccgcgta gtcgatctcc aactcgccgc agccggccac gcagacccgc cccatctggc
     8941 gggcgacaag ggcggcgtgg gaggtcatgc cgccccgggc cgtgaggatc ccctcggccg
     9001 cgttcatgcc cttgatgtcc tcgggcgagg tttcgatccg ggtcaggatg accttctccc
     9061 cgcggccggc ccaggcctca gcgtcgacgg aattgaaaac gatccggccg gaggccgccc
     9121 cgggtccggc gttgagcccc ttcgtcagaa ggcggccggc cttgatcgcg gccgtttttt
     9181 cggccgggtc gaaaatcggc cgaagcagct gattcagctg atccggttcg atccgggaca
     9241 gggcttcctt ctcggagatg agcttttccc cgaccatgtc cacggcgatc ttgatcgcgg
     9301 cgaaggcggt gcgcttgccc acccggcact ggagcatgta aagcttgccc tgctggatcg
     9361 tgaactcgat gtccatcatg tcggcataat gcttctccag gacatgacgg aacttttcga
     9421 gctgagcgta cgacttggcg tcgtccttct tcagcatgac gatgggcagg ggggtccgaa
     9481 tcccggccac gacatcctct ccctgggcat tcatcaggta ttccccgtag aagacgttcg
     9541 ccccggtcgc cgcgtcgcgg gtgaaggcca cgcccgtccc ggagtcgggc cccatgttcc
     9601 cgaagaccat gagctggacg ttgacggccg tgccccaggc ctcggggatg tcgtacatct
     9661 tccggtaggc gatggcccgg tcgttcatcc aggaatcgaa gaccgccccg atcgcgcccc
     9721 agagctgttc gcggggatcc tcggggaatt cgagaccggt gtttttcaga atcgcggcct
     9781 tgaaccggac acacaggtcc ttcaagtcgt cggccgtcaa gtccatgtcg gctgtcaggc
     9841 cgcgctccgc cttcttttcc tcgatgatct tctcgaaggg gtcgacgtcc tccttccgga
     9901 cgggcttgag ccccaagacg acgtctccat acatttggat gaagcggcgg tagcagtcgt
     9961 aggcgaaccg gggattgccg gtcttggcga tgagggccgc gaccgtcttc tcgttcaacc
    10021 ccaggttgag gatggtgtcc atcattcccg gcatcgaggc ccgggccccc gagcggatgg
    10081 aaacgaggag gggattcttg gaatccccga gggtttgacc ggccgctttt tccagcttct
    10141 tgaggttttt atcgatctgg gcccggacat cggccggaat ccttttgcgg ttcttgtaga
    10201 agagatcgca gacttccgtc gaaacggtga agcccggcgg aacgggcagg cccagcccgg
    10261 ccatctcggc caggttagcc cccttgccgc cgaggatgtt cttcattttc atgtcgcctt
    10321 cggccccacc ggcgccgaag aagtagacgt atttacggct catggcagtc tccttgcaaa
    10381 tcgcgggaat atagtcgcgg aaatatacaa taaatcccga agcgcggtca aacccgtgtt
    10441 tcggcgggaa aagccctatt tctcgaccgt ggccttgtac ttttccaggc cggcccggaa
    10501 gacgaccaaa gcccggcgga ggtcatcctc attaaggaca tacgccgccc ggatctcgtc
    10561 ctttcctttc cccggcgtgc cgtaaaagcc cgattccggg gcgaccatga ccgtctggcc
    10621 gtccaggcgg aagtcggtca gcatccagat gacgaatttc tccgtgtccc ggacgggaac
    10681 gcggacgctc atgtaaaacg ccccctccgg cttgcggacg acgactccgg gaatggccga
    10741 caacccttcg aaaaggacgt cgcgccgccg ctcgtattcc cgccggacgg gctcgaagta
    10801 gcccatgccc atcttgtagg cggcgatggc cgcccgttgt tcgatcgtcg gcgggcacag
    10861 ccgggcttgg gcgaacttga gcatcgccgc gtacacctga tcgttccggg tgatggccgc
    10921 tccgacccgg gcgccgcagc agctgaaccg cttggaaatc gagtcgacga cgatgacccg
    10981 gtcccgggcc tcctcaaatt ccagcaggct gatatgccgc cggccgttgt agacgaattc
    11041 cttgtagacc tcgtcgccga tgatgaacag attgtgcttg accgccagct tgacgacccg
    11101 gccgagctcc tcggcggtgt agacggtgcc ggtcgggttg ttgggcgagc acaggacgat
    11161 cgcccgggtc cgaggcgtga tcttggcctc gaattcctcc gccggcggga ggcggaaccc
    11221 gtcctcgacc ttgagcgtca gggggacgac cttgagggcg gccagcgagg cataaccgtt
    11281 gtaattcgtg tagaacggct ccggaatgat gatttcgtcg ccgggatcgg cgaccgtggc
    11341 gaaggccatg aggatggcct ccgagccgcc gagagtgatg aggacgttgt cggccttgag
    11401 gctgatcccg taatccttga aatagccggc gatggcttgc ctcagctcca ggaatccctg
    11461 ggccggccca taacccagaa tggggtcgtt gaagctccgg acggcgtcca gaatcggctt
    11521 gggcgtcggg atgtcggggt cgccgatatt gagaaagaaa attttaacgc cgtcggcgat
    11581 cgccgcgtcg gcataaggct tgaacttacg gatgggtgag gcctggaccg tttggccccg
    11641 tcgagagata tccatggcga actcctttac cgggaaatcg taggaaatgg aagaggtgat
    11701 tatggcccaa atccccccat tctgtcaaag gatccgccca aaaaacggtc tcttgcggag
    11761 gccggctcgc tggagttaaa ctgttttccg ccatgaatct ggcgccggac gctgttctgg
    11821 ggcattaccg cctggccgag aaaatcggcg aaggcgggat gggagtcgtc tggaaagccc
    11881 gcgacttgcg tctcgaccgc gacgtcgccg tcaagttcct gtccgaaggc ccgtctctgt
    11941 cgcccgagcg cagatccctc ctggagcgcg aagcccgggc ggtcgccgcc ctgcgccacc
    12001 ctaatatcgt cacgatttat tccatcgagg aattcgaaga cgccctcttc tacaccatgg
    12061 agctcgtcga ggggacgccg ttgaccaagc tcatcgttcc cggcgggatt gagttcgacc
    12121 gtctcctggc cgtcgccttc cccctgaccg aggcgatcgc cgccgcccac gaccgcggca
    12181 tcatccaccg cgatctcaag cccggcaacg tcatgatcga gccggacggg aagctcaaaa
    12241 tcctcgactt cgggctggcc cgggttctcg agccggtcct tcctcccctg acggccgggg
    12301 gcgaggcgga caccgagacg gcggaaacgg gcatcaccgg gacgctttgc tacatgtcgc
    12361 ccgagcagat ccgcggagaa gccgtcgatc accggaccga tctcttttcc ttcggcgtcg
    12421 tcctcttcga gctggccacg ggcatccttc cgttcagcgg gaacacggcg gccgacctga
    12481 tctcctcgat cttgagggac gagccccgct cggcggtcaa ggccaatccg cgccttccgg
    12541 cctcaatcga ccggattttc ggcgggttgc tcgaaaagga cgcccggtcc cggatcgcct
    12601 ccgctcgaag cctccgggat tctctggaag cgctccgccg cgaaagcgcc ggccggcagg
    12661 gcgaagagat cccttcgatc gccgtccttc cccccgtcga catgagcccg gagaaggacc
    12721 aggcctactt ctgcgaggga atcgccgagg agatcatcat cgccctcggc cgggtgaagc
    12781 aactccgggt ggcctcgcgc tcagcctcgt ttcagttcgc gggcggatcc gtagaccccc
    12841 gggagatcgg ccggcgtctc aatgtcggta ccctgctcga gggcagcgtt cgaaaagacg
    12901 gcagccggct ccggatctcg gcccaattgc tggacaccct ccacggcttc cagctctggt
    12961 cggagatctt cgaccgggag cacggggata tcttcgccat ccaggaggag atcgcccgga
    13021 acgtcgtccg agccctccag gtcaccctca gcccccggga aaaaggggcc ttgggaaaaa
    13081 tctccacccg gcacgtccag gcctacgatt actatcttcg cggacgcgga ttctacaacc
    13141 ggtacaccaa gcaggacatc gagttcgcat ttcagctttt cacccgggcc acggagctcg
    13201 atgacagctt cgcctcggcc ttcgccggcc tggccgattg ctggtcgtac atctttctat
    13261 actcggaaag gaaggaatcg gtccgccgcc aggccgagga ggccgcccgc cgggccgtcg
    13321 agctggacgc cgaatccggc caagcccagg cgtcctacgc cctggccctg tccctcggcg
    13381 gccggggcga ggaggcggtc gaccatttcg aaaaagccgt cgccctggag cccggcctgt
    13441 tcgagggctg gtactattac gcccgccaca gcttcgccca gggggagtat gaaaaggcca
    13501 tccgcctgta tgaacaagcc tgccgcgtcc ggcccgacga ttaccagtcc cccctgctca
    13561 tggcccagat ctacgaggaa gtcggccggc cggaagacgc ccgcaagtcg cgcctgcgcg
    13621 gaatcgagct ggccgaatcc cgtctcgagc tgcacccgga cgactcccgg gcgctttaca
    13681 tgggcgccaa cggcctcgtc gccctcgggg aaaaggaacg gggcctggaa tgggcccgcc
    13741 gggcccgggc cctcgccccc gacgattcca tgctcctcta caacctggga tgcatctatt
    13801 cgctggccgg cgattacgaa gaggccatgt cctgcctcga aaagtcgatc ggcgtcggcc
    13861 ggatgaccca gaaagcctgg tacgagcacg acagcaacct cgaccccctc cgcggcctgc
    13921 cccggttcaa agagctgctg gacagcttgg aatagaagac cgggcggctt tccatccgcg
    13981 ctgatcgacc ggtcctcttc ctttcgacga ggttccgttc cttg
//
LOCUS       DAOOXH010000063        13886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_4465_length_13886_cov_8.709638, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000063 DAOOXH010000000
VERSION     DAOOXH010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 13886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13886
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4465_length_13886_cov_8.709638"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PKV64_08010"
     CDS             <1..612
                     /locus_tag="PKV64_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPN17060.1"
                     /translation="YEEGGQLTEAVKRRPYALVLLDEIEKAHPDVFNVLLQILDDGRL
                     TDGQGRTIDFKNTIIIMTSNLGSAILKDRSRDWAAAEAEVKTLVEGHFRPEFINRIDE
                     LIVFKPLSREVIAGIVDLQLDLLRKRLADRKIGVETTAKARALVAEMGYDPVYGARPL
                     KRVIQKEIQNVLAMKLLAGEIRGGDTVKIDVDGKGGFIFSTNP"
     gene            782..2062
                     /locus_tag="PKV64_08015"
     CDS             782..2062
                     /locus_tag="PKV64_08015"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HPN17061.1"
                     /translation="MKKIIWTICLITAAAAAPAAYANGLNLNGLGARAVTMGGAFVGL
                     ADDYSAIFWNPAGLGFMDKPAIGFNLFDVMPSGSYRFAIPGGEVKAESPTKHYLGGLG
                     GWIQPVNDRLVAGIGVFTPSGLGAAWKSKDMASLSGGQTSIDWSSRIGVFTFAPAVAY
                     RAGDSLSFGARIDISYGFFNLSTYAGSIDAGPENVIDLGQYEETLNGWGFGAAVGVLY
                     KPGPNVSLGVTYRTPATVRFAGKAAISMFKYLGYPEESEIDRPVTWPSWLAAGAAVKP
                     VDGLTLTADVQYTNWKAMDVLETTFKEPFWKQIMTAAGNTRRELYWEDKIQVRFGAEY
                     VLGKEWAVRAGYYSDPSPAPDRTMNILLPSYGFSGVTLGFGRSSGGLRIDFGVELLLA
                     KDRTIAATRENAAAMPGVYGMTILVPHFSVSHRF"
     gene            complement(2467..2539)
                     /locus_tag="PKV64_08020"
     tRNA            complement(2467..2539)
                     /locus_tag="PKV64_08020"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2504..2506),aa:Phe,seq:gaa)
     gene            2952..3926
                     /locus_tag="PKV64_08025"
     CDS             2952..3926
                     /locus_tag="PKV64_08025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPN17062.1"
                     /translation="MKIAALLPHVQVFGGVRRYLELGNEFVRKGHSFTLFHPAGSAPG
                     WLEFIGEPRPFSALEEDSFDAALCGEYAILPYFDRIRARDKYFYFVLEGHKMEREVAG
                     RPYRFLGNSEGICRRLERKYGLKPIRAPGGVNPAIFHPLDRPEERETFNVLCYGRIYK
                     RRKGVPIVIRAVESLARRHPNLKLVFFDTLVGDDRRDPRPLIKTRIPFDFHMNLPQDK
                     MAWLFGQADVFATAERRAGWANTAAEAMACGVPVVCTPSGSRDFAEDGRTALVAPYPL
                     AGPIRRRIERLIVDGPLRRRLSEAGTARIAEFTWSRLADRLIEVFSRG"
     gene            4202..5230
                     /locus_tag="PKV64_08030"
     CDS             4202..5230
                     /locus_tag="PKV64_08030"
                     /inference="COORDINATES: protein motif:HMM:NF014793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HPN17063.1"
                     /translation="MAKTKSKHRLALVGTDSLRGQEIKNILAQKKLPAFDIELFDPDV
                     KENFSKLTQFKKEPMVVHRLEPGALEGKDLVFLAADAKTSKEMGKLAEAGRFKAIDLS
                     ETFNDREDVPLLVSGVNDDSLKTAIPPLLANPHAVTIILASLFHRIIPKFGLKRAVSF
                     VLRPVSAFDDPGIQELASQSVALLNSSVPKTKVFKEQIAFNILSHTDALDSDGFSVLE
                     RRLVAEIRRVLGRPEFPLVLSIIQAPVFHTYSVMTYFELKDEAESADIEGLFKSAPFK
                     MTPFRTSCEATPLKVSGKDEIFVGQVKREPDNPRAFWIWLVADNLTRGAALNAFDLAR
                     KILGAETR"
     gene            5241..7058
                     /locus_tag="PKV64_08035"
     CDS             5241..7058
                     /locus_tag="PKV64_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN17064.1"
                     /translation="MFELLKLTVREKKWVTLALLSTLGVAFFTAVMMNLVQPVIDEMF
                     GANPSASAAAADGAAATKVRALDFLWNLLGIDRGDIKSALPLVLIIVIFGKGLFTFLS
                     SFFMKAVGNKVVKQMRDKLYAHILGMSSGFFDRATTGDLMSRLTNDVDRIQQAVGTAM
                     SDMVEESFVLAGLLLTLLINDWQMALVAFVIAPLAAIPMAAFSRQLKKQGRKSQLKMA
                     DIYNLIHETVTGNRIVKAFTMEGFELRKFLKSSWRYYRINLKLAWISSLSSPFMEFIG
                     GLVGAFILVVGADRIAKNQISPGDFGVFVSTIFYMFTPIKRLSRANNVVQQAVACYDR
                     VQEVLNTPQEIEDRPEAYPLPPVQGRVKFDRVGFAYLTDRPVLFDVSFETKPRETIAL
                     VGLSGAGKTTIVNLIGRFYEATSGTITIDGNNVRDVTLASLRSQIGLVTQEIVLFNDT
                     VRANIAYGLDDVPLEKIIASATAAECHEFISELPQGYDTLIGERGGILSVGQRQRLAL
                     ARALLKDPPILILDEATSALDSESERLIQQALTRIMKDRTTFVIAHRLSTIRNADAIL
                     VIDGGRIVESGTHEELIRKPRGIYRKLHDLQFPEEENGG"
     gene            7055..7933
                     /locus_tag="PKV64_08040"
     CDS             7055..7933
                     /locus_tag="PKV64_08040"
                     /inference="COORDINATES: protein motif:HMM:NF015699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HPN17065.1"
                     /translation="MIQSMTGFAEKSFSTRTLRVKICLKSLNHRFFDWSYKGAPIGEL
                     ENRLREICQERIHRGRIEAMIDMAFPDPASWDVVINEGLLKKILAAFDRVAVRSGHEL
                     TFGLDSLFRIPQLVELRRKDFTPAETEWIAAGFKKTLDELLKSRRAEGRKIAAALQTH
                     LRAIAVSVGRVAVLIKKQPRTLQKKLRARLAEANGAGPNSKERMAEEVAYLSQRYDVA
                     EEIARLKHHLEAARELLSVKNRAPAGKMLDFLAQEFTREANTLNSKSQEIGVTREGLL
                     IKGEVESIRQQVQNIE"
     gene            7944..8552
                     /gene="gmk"
                     /locus_tag="PKV64_08045"
     CDS             7944..8552
                     /gene="gmk"
                     /locus_tag="PKV64_08045"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPN17066.1"
                     /translation="MLFVVTGPSGCGKSTILAQLVKDVRSISFSVSHTTRPRRPSEAE
                     GRQYYFVSETEFKRMIKKDMFLEWAEVHGHCYGTSKREIAARVKEGDVILDVDVQGAR
                     SIRTLRKDATFVFVLPPRAADLRKRLLDRGEDDNASIRRRLNNAKREIREASSFDYVI
                     VNDRLNRAVLELEAIVLAARCRAESQAAKIRAVLRSFPAAKK"
     gene            8559..9755
                     /gene="coaBC"
                     /locus_tag="PKV64_08050"
     CDS             8559..9755
                     /gene="coaBC"
                     /locus_tag="PKV64_08050"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPN17067.1"
                     /translation="MAKIALGVASSISIYKACEILRGFQKEGHDVQVIETRNAAALIP
                     PALFDALSGRRTIVELFDDRQPWSVAHVALAKEIALFVVAPATANILAKFACGLADDF
                     LSTFYLAVRTPVLIAPAMNEAMWIHPQTQDNVRRLRERGVEFVEPAGGYLACGDEGQG
                     RLADPQRIIARGLDLLCRGTGLRGKKVLVTAGPTREAMDPVRYLSNRSSGKMGFEVAR
                     EALRRGADVVLIAGPVAIAPPSGLEVVPVTSAAEMKEAVDKHFSGADVTVMAAAVADF
                     MFRAPAVRKVKKETLPAGLEIVRTPDILKELGGRKRPGQILVGFAAETNAVEAAARKK
                     LREKNLDLIVANDVSREGTGFEADVNQAVLIDRRGKSRKTDILSKRALAAVIWDAVEG
                     LREKKK"
     gene            9739..10491
                     /locus_tag="PKV64_08055"
     CDS             9739..10491
                     /locus_tag="PKV64_08055"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPN17068.1"
                     /translation="MKRKSDALSALADRVRFFEEIGAGFLFRSEEPRPEAGAAGETIV
                     AGSPRPAARPAESGLDLPRVDRTLDDVSAEIRACRKCSLAAGRTKAVPGEGNPKSPLM
                     FVGEGPGHDEDVQGRPFVGRAGQLLTKIIAAMGYERSEVFIANVVKCRPPENRVPHRE
                     EAEACAPFLIEQISILRPRVIVALGKTAVDFFVPNVKAMGAVRGVFQEWHGLPVMPTF
                     HPSYLVRNEGNREIKRMVWNDMQKVMAFLGGK"
     gene            10488..12440
                     /gene="priA"
                     /locus_tag="PKV64_08060"
     CDS             10488..12440
                     /gene="priA"
                     /locus_tag="PKV64_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPN17069.1"
                     /translation="MTLFVDVAFPRPLLRSYTYAIPAALSGRVRPGVRVRASLGRQTA
                     VGVAVRVHDEPPAGEAAVKEIAAVLDEAPALPLPILAFTRKLAAEQAAGWGALLGLAL
                     PPDPAPPLKRRAAEKGRVEVEQLELDLESTAAVAAAAAEIGRRIGAGERAEFLAWGGR
                     GAGRAMIIEGVRRALDHSCNVLVLLPDIARAGDLRSRFQAALGTAPALLHGQMTPRAQ
                     RAEWERVERGDARVILGSRAALFKPWPRIGLIVVEDESDESHGQAGFPSYDVRNGARL
                     RSTEENAVLLLVAEAPTVSAYARARAAGALFSLGGEEPPPGVRVVDDSTGKDVLTEAM
                     REGILGCLAAGGRAVLFLNRRGYASLLFCPRCGFIPRCGRCGSIQPYHKKADRLVCRS
                     CGAEDKRPSSCPSCRHRVLEPRGGGVEALEEELGGVFPGARIAVFDRDRARRAAERKK
                     ILDAYGRGRLDLLVATQLLVRQPGLPPADFVGVFNPESGLAFPDFLAPERTFQSLLRM
                     IRFARTGVRPGRVVIQTGFPGHYGIRTASRADFEAFFSEEIELRRLFRRPPFTASADV
                     VLSGRDARLLGRQSRALAAVWREADPKVEVLGPSFATEPGRPGIKKIQLELRSDSAEA
                     ITALLAAGLEDFGRDVQVVRHDGFIR"
     gene            complement(12430..12882)
                     /locus_tag="PKV64_08065"
     CDS             complement(12430..12882)
                     /locus_tag="PKV64_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17070.1"
                     /translation="MPRTQAFKITGLILLVALAAACQRTAGPSPTPVGPSTFALTFSL
                     TANPNVILTGTARPTSTIEAAVTENGEPAFNRTVYFTIADGPGEFPNFSQRAAVTTDW
                     NGVASIAYTGPTKFEIDSDTSATVMAQLETSSPQIIAKTVVITILFSE"
     gene            complement(12894..13511)
                     /locus_tag="PKV64_08070"
     CDS             complement(12894..13511)
                     /locus_tag="PKV64_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17071.1"
                     /translation="MRYSPSPVQTVRILALSALLLAAAACNPLENKTQSMSYLVIENL
                     MGLDESGKVADYVASDVLFQDPDTGDTSIIADIATATISARQLDPDPIAGTSPYADVQ
                     LTHYTVTYTRSDGRNKPGVDVPYPFDGDLTVLLKVNIATEFGFIIVRESAKQEPPLLD
                     LLQGGSRAEIIYTTATVDFYGHDLTGAEVKVTGAISVRFANFANG"
     gene            complement(13522..>13886)
                     /locus_tag="PKV64_08075"
     CDS             complement(13522..>13886)
                     /locus_tag="PKV64_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HPN17072.1"
                     /translation="ANFTVTTQYVNAGLELRATPQITADGTIIMTIDIQNNAADFSTT
                     AGGIPSITTQSAKTTVMVPDGGTTVIGGIFRVEDSITRERVPFLHQIPILGNLFKSLS
                     KNRTNREVLIFITPRIQK"
BASE COUNT         2477 a         4325 c         4500 g         2584 t
ORIGIN      
        1 tacgaggagg gaggacagct gaccgaggcc gtcaagcggc ggccctacgc gctcgtcctg
       61 ctggacgaga tcgagaaagc ccaccccgac gtcttcaacg tcctgctcca gattttggac
      121 gacggccgcc tgaccgacgg ccagggccgg acgatcgatt tcaagaacac gatcatcatc
      181 atgacctcca acctggggag cgcgattctc aaggaccggt cccgggactg ggcggccgcg
      241 gaagccgagg tcaagacgct cgtcgagggg catttccggc cggagttcat caatcggatc
      301 gacgagctca tcgtcttcaa gccgctgtcc cgggaggtca tcgccgggat cgtcgacctc
      361 cagttggatc ttctccgcaa gcgcctggcc gaccgcaaga tcggcgtgga gacgacggcc
      421 aaggcccggg cgcttgtggc cgagatgggc tacgatccgg tctacggcgc ccggccgctc
      481 aagcgcgtca tccaaaagga gatccagaac gttctggcca tgaagctcct ggccggggag
      541 atccggggcg gggacacggt caaaatcgac gtcgacggga agggcggatt tattttttcg
      601 acgaatccct aaggaaaatc cctaaagcgg ccgtccgccc tcaggatcgc cgggggccgg
      661 ccggcggggc gtgtcgtccg gggccgccgg ccgcgatcgc cggcgtcgat cggcggattg
      721 tctttgtcga cgaatcatag taaattgaat tccgtccgaa tttcgatcaa gggtgcccct
      781 catgaaaaaa ataatatgga cgatctgttt gatcacggcc gcggccgccg cgcccgcggc
      841 gtacgccaac ggcttgaacc tcaacgggct cggcgcgcgg gcggtgacaa tgggcggcgc
      901 gttcgtcggc ctggccgatg attacagcgc gatcttctgg aatccggccg gtctcggctt
      961 catggacaag ccggccatcg gcttcaacct gttcgacgtg atgccgtccg ggtcctaccg
     1021 gttcgccatc cccggcggcg aggtgaaggc cgagtcgccg accaagcatt acctgggcgg
     1081 cctgggcgga tggatccagc cggtcaacga ccgcctggtc gcggggatcg gcgttttcac
     1141 gccctcgggg ctgggagcgg cctggaaatc gaaggacatg gcctcccttt ccgggggaca
     1201 gacgtccatc gactggtctt cccggatcgg cgtcttcacg ttcgccccgg ccgtcgccta
     1261 tcgggcgggc gattcgctct cgttcggcgc ccggatcgac atttcctacg gctttttcaa
     1321 cctgagcacc tacgccggtt ccattgacgc gggtccggaa aacgtgatcg acctggggca
     1381 gtacgaggaa accctgaacg gatggggctt cggagcggcc gtgggcgttc tgtacaagcc
     1441 cggcccgaac gtcagcctcg gggtgaccta ccggacgccg gcgacggtcc gctttgccgg
     1501 caaggcggcc atctccatgt tcaaatacct gggctatccg gaggagtcgg agatcgaccg
     1561 gcccgtcact tggccttcct ggctggcggc tggagcggcc gtcaagccgg tggacgggct
     1621 gaccctcacg gccgatgtcc agtacacgaa ttggaaggcg atggacgtct tggagaccac
     1681 cttcaaggag ccgttctgga agcagatcat gacggccgcg ggaaacaccc ggcgggagct
     1741 gtactgggag gacaaaatcc aggttcggtt cggcgccgaa tatgtcctgg gcaaggaatg
     1801 ggccgtccgg gcggggtatt acagcgatcc ctcgccggct cccgatcgga cgatgaacat
     1861 cctcctgccg agctacggtt tttcgggcgt gacgctcggc ttcggccggt cgtcgggagg
     1921 gctgcggatt gatttcggcg tggagctgct cctggccaag gaccggacga tcgccgcgac
     1981 gagggaaaac gccgcggcta tgcccggcgt ctacgggatg acgatcctcg tcccgcattt
     2041 ctccgtgagc caccggttct gaatcggcgg gcgggagcgg cggccctcgg aatttccgga
     2101 cggactgcgg cccatatccc ggcccaactc ctaattcaac gactccccca agcggagagg
     2161 ggaaaaccgg cgggcggccc gaaaaacccg gctcgaaaaa gggggccgca atccctcgat
     2221 gcggcgcggg tgtttgccga gatcgtcaat gcgccggccg gaattgtacg cggggggata
     2281 tcattcggaa gccgggtcgg cctcgccttg agggggaaag cggatcatag gattaacgcc
     2341 gtgtcatttt acggtgccgg gccgtcgcgg aaatcaaatc gatgtcatta tgatcaaccg
     2401 atgttgtccg gccgacgggg gacgaaatct ctgtattttg aatgaattat gcggggaaat
     2461 aggaaatggc tagggacgga attgaaccgc cgacgcaagg attttcagtc cttcgctcta
     2521 cctactgagc tacctagcca tccatcgaaa gcgaaaagaa gcgagcctaa gtatattttt
     2581 ttaggccggg attgtcaacg gaaagcccgg ctggttcgaa cccggcctca agttccgccg
     2641 gagaggattt ctttatattt tttccgggaa gctttcaggc ggatcttgcc cgcgagagac
     2701 ggtccccgac ggcgttgggg ccggtttgtc cgtctcgatg accggcgttg aagtctttgg
     2761 tttttttgaa gagatccgcg gctttttaaa gccgagcggc ttgaattcga ccagaatcct
     2821 aatccgcaat caatccccct tcggcaagtt aatcaatatc gggggatccg tcaaggcgga
     2881 tttttttcga ggccgggccc ggactggcgg gaagggacgg ccgtttgtta gaatagcctc
     2941 gatcatccgt catgaaaatc gccgccctcc tgccccatgt ccaagtgttc gggggcgtcc
     3001 gccgctatct cgagctgggg aacgaattcg tccgcaaggg ccattccttc accctgtttc
     3061 accccgccgg ctccgcgccc ggatggcttg aattcatcgg tgagccgcgg ccgttttccg
     3121 ccctggagga ggactccttt gacgcggccc tgtgcgggga atacgcgatc cttccgtatt
     3181 tcgaccggat ccgcgcccgg gacaagtact tctattttgt cctcgagggt cacaagatgg
     3241 agcgggaggt cgccggccgg ccttaccgtt tcttgggcaa ttccgagggg atttgccgcc
     3301 gcttggaacg gaaatacggc ctgaagccga tccgggcccc cggcggagtc aacccggcga
     3361 tttttcatcc cctggaccgg ccggaggaac gggagacgtt caatgtcctc tgctacggcc
     3421 ggatctacaa gcgccgcaag ggcgttccca tcgtcatccg ggccgtggaa agcctggccc
     3481 gccgccatcc gaacctgaag ctcgtctttt tcgacaccct ggttggagac gatcgccgcg
     3541 atccccgtcc cttgattaaa acgcggattc cctttgattt ccatatgaac ctgccccagg
     3601 acaaaatggc ctggcttttc ggacaggcgg acgttttcgc gacggccgaa cggcgggccg
     3661 gatgggccaa caccgcggcc gaggcgatgg cgtgcggcgt tccggtcgtc tgcacgccca
     3721 gcggaagccg cgatttcgcc gaggacggcc ggacggccct cgtcgccccg tatcccctgg
     3781 ccggcccgat ccggcggagg atcgagcgct tgatcgtcga cgggccgctt cgccgccgtt
     3841 tgtccgaggc cgggaccgcc cggatcgccg aattcacttg gtcccggctg gccgaccgcc
     3901 tgatcgaggt tttttcccgc ggctgagcgg acgcctccgc ttcacgcggc cgaggccgcg
     3961 gccgtcgaaa atcacggtcg tccgcgatag gtcgtttcca ggattttttc gggaatgaca
     4021 tgaagcgtta ttctggacaa taatgaaacc gggccggacc tctgagaaac gcggccataa
     4081 aacaggacga aggggcggcg gccgcgatat acgtaatata tcttttaggc cggaactcga
     4141 aaaaaggccg aaaattgata ttcccggggg gattgtgata ttctgacgcc ctcgattctc
     4201 catggcgaaa acgaaatcaa aacaccgtct ggcgctcgtc ggaaccgact ccctccgcgg
     4261 gcaggaaatc aagaatattc tggcgcaaaa gaagcttccg gccttcgata tcgagctgtt
     4321 cgatcccgac gtcaaggaaa atttcagcaa gctgacccag ttcaagaagg agccgatggt
     4381 cgtccatagg ctggagccgg gcgccctgga ggggaaagat ctcgttttcc tcgcggccga
     4441 cgccaaaacc tcgaaggaga tgggcaagct ggccgaagcg ggccggttca aggccatcga
     4501 cctcagcgag acgttcaacg accgggagga cgtgccgctc ctcgtctccg gggttaacga
     4561 cgattcgctg aagaccgcga ttccgcctct cctggccaac ccgcatgccg tgacgattat
     4621 cctggccagt ctgtttcacc ggatcatccc gaaattcggc ctgaagcggg ccgtgtcctt
     4681 cgtcctccgg ccggtctcgg ctttcgacga tccgggcatc caggagctgg ctagccagag
     4741 cgtcgcgttg ttgaacagct cggtgcccaa gacgaaggtc ttcaaggaac agatcgcctt
     4801 caacattctc tcccacaccg acgccctaga cagcgacggc ttttccgtcc tcgagcgtcg
     4861 gctggtggcg gaaatccggc gcgtcctcgg tcggccggaa ttccccctgg tcctgtccat
     4921 catccaagcc cccgtattcc acacgtactc ggtgatgacc tacttcgagc tcaaggacga
     4981 ggccgaaagc gcggacatcg aggggctgtt caaatccgct cccttcaaga tgacgccgtt
     5041 ccgcacctcc tgcgaagcca cgcccttgaa agtctccggc aaggacgaga tttttgtcgg
     5101 gcaggtcaag cgggaacccg acaatccccg agcgttctgg atttggctgg tcgccgacaa
     5161 tctgacgcgc ggcgcggccc tgaacgcctt cgacctggcc cggaaaatcc tgggggccga
     5221 gacccgctag gcgggccgcg atgttcgaac tcctcaagtt gaccgtccgg gaaaagaaat
     5281 gggtcaccct ggccctgctc tccaccctcg gcgtcgcctt cttcaccgcg gtcatgatga
     5341 acctcgtcca gccggtcatc gacgagatgt tcggagccaa tccatcggcc tcggcggcgg
     5401 ccgcggacgg ggcggccgcg accaaggttc gggccctgga ctttctttgg aatcttctcg
     5461 gcatcgaccg gggggacatc aagagcgccc tgccgctggt cttgatcatc gtcatcttcg
     5521 gcaaaggcct gttcactttt ctctcgtcgt tcttcatgaa ggccgtgggc aacaaggtcg
     5581 tcaagcagat gcgggacaag ctctatgccc acattctcgg catgtcgtcc ggttttttcg
     5641 accgggcaac caccggggac ctgatgtccc gcctgacgaa cgatgtcgac cggatccagc
     5701 aagccgtcgg aacggctatg agcgacatgg tcgaggaaag ttttgtcctg gccggccttc
     5761 tcctgaccct gctgatcaac gactggcaga tggccctcgt ggccttcgtc atcgccccgc
     5821 tggccgccat tcccatggcc gccttcagcc gccagcttaa gaagcagggg cggaaaagcc
     5881 agctcaagat ggcggacatc tacaacctga tccacgagac cgtgaccgga aaccggatcg
     5941 tcaaggcctt cacgatggag ggcttcgagc tccggaaatt cctcaagtcc tcctggcggt
     6001 attaccgcat caacttgaag ctggcctgga tctcgtccct gtcctcgccg ttcatggaat
     6061 tcatcggcgg cctggtcggg gccttcatcc tggtcgtcgg agccgaccgg atcgccaaaa
     6121 accagatcag cccgggcgat ttcggagtgt tcgtttcgac cattttttac atgttcacgc
     6181 cgatcaagcg gttgagccgg gccaacaacg tcgtccaaca ggccgtggcc tgctatgacc
     6241 gcgtccagga ggtccttaac acgccccagg aaatcgagga ccggccggaa gcctacccgc
     6301 tgccgccagt ccaggggcgg gtgaagttcg accgggtcgg cttcgcctac ttgaccgatc
     6361 ggccggtcct tttcgacgtc agcttcgaaa ccaagccgcg ggagactatc gccctggtcg
     6421 gtttgagcgg ggccgggaag acgacgatcg tcaacctcat cggccgcttc tacgaagcca
     6481 cctccgggac gatcacgatc gacggaaaca atgtccggga cgtcaccctg gcttccctcc
     6541 ggtcgcagat cggcttagtg acccaggaga tcgtcttgtt caacgatacg gtccgggcca
     6601 acatcgctta cggcctcgac gacgttcccc tggaaaaaat catcgcctcg gcgacggccg
     6661 cggaatgcca cgaattcatc tccgagcttc cccaaggcta tgacacgttg atcggcgagc
     6721 gcggcggcat tctttccgtc ggccagcgcc agcggctggc cctggcccgg gccctgctca
     6781 aggatccgcc gatcctgatc ctggacgagg cgacctcggc cctggattcg gagtccgagc
     6841 ggttgattca gcaggccttg acccgtatca tgaaagaccg gacgacgttc gtcatcgccc
     6901 atcggctgtc cacgatccgg aacgccgacg ccatcctggt catcgacggc ggccggatcg
     6961 tcgagagcgg aacccacgaa gagctgatcc gcaagccgcg cggcatttac cggaagctgc
     7021 atgatctgca gttcccggag gaggaaaacg gaggatgatt caaagcatga ccgggttcgc
     7081 cgagaagagc ttctcgaccc ggaccctgcg cgtcaagatc tgcctgaaaa gcctcaacca
     7141 ccgctttttc gactggtcct acaagggcgc cccgatcggg gagctcgaaa accgtctccg
     7201 ggagatctgc caggaacgaa tccatcgcgg ccggatcgag gcgatgatcg acatggcctt
     7261 tcctgatccc gccagctggg acgttgtcat caacgaggga ttgctcaaga aaatcctggc
     7321 ggccttcgac cgggtcgccg ttcggtcggg ccatgagctg actttcgggc tggacagtct
     7381 cttccggatt ccccagctgg tcgagctccg aagaaaggat ttcaccccgg ccgagaccga
     7441 gtggatcgcc gccggcttca agaaaaccct ggacgagctt ctcaagtccc gccgggccga
     7501 gggacggaaa atcgccgcgg ccctccaaac tcacttgcgg gccatcgccg tctcggtcgg
     7561 ccgggtggcg gttttgatca agaaacagcc ccggactctt caaaagaagc tccgggcccg
     7621 cctggccgag gccaacgggg ccggcccgaa ttccaaggag cggatggccg aggaagtggc
     7681 ctacttgtcc cagcgctatg acgtcgccga ggaaatcgcc cgcctcaagc accacttgga
     7741 ggccgcccgc gagctgctgt ccgtcaagaa cagggcgccg gccggaaaga tgctcgattt
     7801 cctggcccag gaattcaccc gggaagccaa caccttgaat tccaaatccc aggaaatcgg
     7861 cgtcactcgg gagggactgc tcatcaaggg agaggtcgag agcatccgcc agcaggttca
     7921 aaacatcgaa tgaggaggat gacgtgttat ttgtcgtgac ggggccgtcg ggttgcggga
     7981 agtcgacgat tctcgcccag cttgtcaagg atgtgcgcag catcagtttt tcggtgtccc
     8041 acacgacccg tccccgccgc ccgtcggagg cggaggggcg gcagtattac ttcgtctcgg
     8101 agacggagtt caagcggatg atcaaaaagg acatgttcct ggagtgggct gaggttcacg
     8161 ggcattgcta cggaacctcg aaacgggaga tcgccgcccg ggtgaaggag ggcgacgtga
     8221 ttctcgatgt cgatgtccag ggagcgcggt cgatccggac gctccgcaag gacgcgacct
     8281 tcgtttttgt tcttccgccc cgggcggccg acttgcggaa gcggctcctc gaccggggcg
     8341 aggacgacaa cgcctcgatc cgacgccggc tgaacaacgc caagcgggag atccgcgagg
     8401 cctcgtcgtt cgattacgtg atcgtcaacg accggctgaa ccgggccgtc ctggagctcg
     8461 aggcgatcgt gctcgcggcc cggtgccggg ccgagagcca ggccgcgaaa atccgggccg
     8521 tcctgagatc cttcccggcc gcgaaaaagt gaggcgtcat ggctaaaatc gcgctgggtg
     8581 tcgcgtcatc gatcagcatt tataaggctt gcgaaatcct ccgcggattc caaaaggaag
     8641 gccacgacgt ccaggtcatc gaaacccgga acgccgcggc cttgatcccg ccggctttat
     8701 tcgacgcctt gtccggccgc cggacgatcg tcgagctgtt cgacgaccgg cagccctggt
     8761 ccgtcgccca cgtcgccctg gccaaggaga tcgctctgtt cgtcgtcgct ccggccacgg
     8821 ccaacatcct ggccaagttc gcctgcggct tggccgacga tttcctgtcc acgttttact
     8881 tggccgtccg aacgccggtc ctcatcgccc cggccatgaa cgaggcgatg tggatccacc
     8941 cgcagacgca ggataatgtt cggcggctcc gggagcgggg cgtcgagttc gtcgaaccgg
     9001 ccggggggta cctggcttgc ggcgacgaag ggcaaggccg cttggctgat ccccaacgga
     9061 tcatcgcccg ggggctggac cttctctgcc gggggaccgg cctccgggga aaaaaagtcc
     9121 tggtgacggc tggaccgacc cgtgaggcga tggatcccgt ccgctacctg tccaaccggt
     9181 cttcgggaaa aatgggcttc gaagtcgccc gggaagccct ccgtcgcggg gccgacgtcg
     9241 tgttgatcgc cggtcccgtc gcgatcgcgc cgccctcggg actcgaggtc gttccggtga
     9301 catcggcggc cgagatgaag gaagccgtcg acaagcattt ttccggcgcc gacgtcacgg
     9361 tgatggccgc ggccgtcgcc gatttcatgt tccgcgcccc ggccgtccgg aaggtcaaga
     9421 aagaaaccct cccggccgga ttggagatcg tccgcacgcc cgacatcctc aaggagctgg
     9481 gcgggcggaa gcgccccggc cagattcttg tcggtttcgc cgctgaaacg aacgcggtcg
     9541 aggccgccgc ccggaaaaag ctccgggaga agaatctcga cctgatcgtc gccaacgacg
     9601 tctcccggga gggaaccggc ttcgaggccg acgtcaacca agccgttctg atcgaccggc
     9661 gggggaaatc gcggaagacg gacatcttga gcaagcgggc cctggccgcg gtgatctggg
     9721 acgctgtcga gggacttcgt gaaaagaaaa agtgacgcct tgtcggcctt ggccgaccgc
     9781 gtccgatttt tcgaggaaat aggagccggg ttccttttcc ggtccgagga gccgcgtccg
     9841 gaagccggcg cggcagggga gacgatcgtt gccggctccc cgcggccggc cgcccgcccc
     9901 gcggaatccg gtctcgactt gccccgcgtc gaccggacgc tcgacgacgt ctccgccgag
     9961 atccgggcct gccggaaatg ctccctggcc gccgggcgga cgaaagccgt gccgggcgaa
    10021 ggcaatccga aatcgccgct gatgtttgtc ggggaagggc cggggcacga cgaggacgtc
    10081 caggggcggc cgttcgtcgg ccgggccggc cagctgctga ccaagatcat cgcggccatg
    10141 gggtatgaaa ggtccgaggt cttcatcgcc aacgtcgtca aatgccggcc gcccgaaaac
    10201 cgggtccctc accgcgagga ggccgaggcc tgcgcgccgt tcctcatcga gcagatctcc
    10261 atcctccgtc cccgggtcat cgtcgccctg ggaaaaaccg ccgtcgattt tttcgtgccg
    10321 aacgtcaagg ccatgggcgc ggtccggggc gtattccagg aatggcacgg ccttcccgtc
    10381 atgcccacct tccatccgtc ctacctcgtc cgcaacgagg ggaaccggga gatcaagcgg
    10441 atggtctgga atgacatgca gaaggtgatg gctttccttg gcggaaaatg accctgttcg
    10501 tcgacgtcgc gtttccgcgg cctctcctcc gcagctatac ttacgcgatc cctgcggcct
    10561 tgtccggaag ggttcggccg ggcgtccggg tgcgggcttc gctcggccgg caaaccgccg
    10621 tcggcgtcgc cgtacgcgtt cacgacgagc ctcccgccgg cgaagcggcg gtcaaggaga
    10681 tcgccgccgt cctggacgaa gcgccggccc ttccgcttcc cattctcgcg ttcacccgga
    10741 agctggccgc cgaacaggcc gccgggtggg gtgccctcct tgggctggcc ctgccgccgg
    10801 accccgcccc gccgctcaag cggcgggcgg ccgaaaaagg gcgcgtggag gtcgaacagc
    10861 tcgagctcga ccttgagtcg accgcggccg tcgccgcggc cgccgcggag atcggacgcc
    10921 gaatcggggc cggcgaacgg gccgagttcc tggcctgggg ggggcgggga gccggccggg
    10981 cgatgatcat cgagggcgtc cgccgggctc tcgatcattc ctgcaatgtc ctcgttttgc
    11041 tgcccgacat cgcccgggcg ggggatctgc gctcccgttt tcaagcggcc ctggggacgg
    11101 cgcccgcgct tctccacgga cagatgacgc cgagggccca aagagccgag tgggagaggg
    11161 tggaacgggg ggacgcccgg gtcatcctcg gatcgcgggc ggccttgttc aaaccatggc
    11221 cccggatcgg tctgatcgtc gtcgaggacg agtcggacga atcgcacggc caagcgggtt
    11281 ttccctccta tgatgtccgg aacggggcgc gattgcggtc gacggaggaa aacgccgtcc
    11341 ttttattagt cgcggaggcc ccgaccgtga gcgcttatgc ccgagcccgg gccgccggcg
    11401 ccctttttag tctcggcggc gaagagcctc cgccgggagt ccgggtggtc gacgattcga
    11461 ccgggaagga tgtcctgacc gaggcgatgc gggagggcat cctgggatgc ctggcggccg
    11521 ggggacgggc ggttcttttc ctcaaccgcc ggggatatgc ctcgcttctt ttctgtcccc
    11581 ggtgcggctt catcccgcga tgcggccggt gcggttcgat tcagccttat cacaaaaagg
    11641 ccgaccggct ggtctgccgc tcctgcggcg ccgaggacaa gcggccttct tcctgcccgt
    11701 cctgccggca tcgagtcttg gagccccggg gcgggggagt ggaggctctc gaagaggagc
    11761 tgggcggcgt ttttcccgga gctcggatcg ccgtcttcga ccgggaccgg gcccggcggg
    11821 ccgccgagcg gaagaaaatc ctcgacgcct acggccgcgg ccggctcgat ctcctcgtgg
    11881 ccacccagct tttagtccgc cagccgggcc tgccgccggc cgatttcgtc ggcgttttca
    11941 accctgagtc cggcttggct tttcccgatt ttttggcccc tgagaggacg tttcaatcgc
    12001 ttctccggat gatccggttc gcccgaacgg gcgtccggcc gggacgggtg gtcatccaga
    12061 ccggttttcc cgggcactac ggcatccgga ccgcctcccg ggcggatttc gaggcgtttt
    12121 tctccgagga aatcgagctt cgccgtctct tccgccggcc tccattcacg gcctcggcgg
    12181 acgtcgtctt gagcggccgg gacgcccgtc tgctgggacg acagtcccgg gccctggcgg
    12241 cggtttggcg ggaagcggat ccgaaggtcg aagtcctcgg gccttcgttc gcgacggagc
    12301 cgggaagacc gggaatcaag aaaatccaac tggagctgcg gtcggattca gccgaggcga
    12361 tcaccgctct tctggcggcc gggctcgagg atttcggccg ggacgttcag gtcgtccggc
    12421 acgacggatt tattcgctga acaggatcgt gatgacgacc gtcttggcga tgatctgagg
    12481 agacgaggtc tcgagctggg ccatcaccgt ggccgatgta tccgagtcga tttcgaattt
    12541 cgtcgggccg gtgtaggcga tcgaggcgac gccgttccaa tcggtggtga cggccgcccg
    12601 ctgggagaag ttggggaatt cgccgggccc gtcggcgatg gtgaagtaca ccgtccggtt
    12661 gaaggccggt tccccgtttt ccgtgacggc ggcctcgatg gtcgaggtcg gccgggcggt
    12721 ccccgtcaag atgacgttgg gattggccgt gaggctgaac gtcagagcga aggtcgaggg
    12781 tccgaccggc gtggggcttg ggccggccgt ccgctggcaa gccgcggcca gggcgacgag
    12841 gagaatcaac ccggtgattt tgaaggcttg ggttcggggc atgcgtatcc tcctcatccg
    12901 ttcgcgaaat tcgcgaaccg gaccgaaatg gctccggtga ccttgacctc ggcgccggtg
    12961 aggtcatggc cgtaaaaatc caccgtggcc gtggtataga tgatttcggc ccggcttccc
    13021 ccttggagaa gatccaggag ggggggctcc tgcttggctg attcccggac gatgatgaag
    13081 ccgaactcgg tggcaatgtt cactttaagg agaacggtca ggtctccgtc gaacggatag
    13141 ggaacgtcga cgccgggctt gttccggccg tccgatcgcg tgtacgtgac cgtgtaatgc
    13201 gtcagctgca cgtcggcgta aggggacgtc ccggcgatgg gatcggggtc gagctgccgg
    13261 gcgcttatcg tcgcggtggc gatgtcggcg ataatggatg tgtcgcccgt gtcgggatcc
    13321 tggaagagga cgtccgaggc gacgtaatcc gcgactttcc cgctctcgtc gagccccatc
    13381 aagttctcga tgacgagata gctcatggac tgggtcttgt tttccagcgg attgcaggcg
    13441 gccgcggcga ggaggagggc cgatagggcg agaatccgga ccgtttggac gggggatgga
    13501 gaatagcgca tcgtcggcct cctatttctg gatcctcggg gtgatgaaga tcaggacctc
    13561 gcggttcgtc cggttcttgg acaggctctt gaacaagttc ccgaggatgg gaatttggtg
    13621 gaggaaggga acccgttccc gcgtgatcga gtcttccacc ctaaaaatcc ctccgatcac
    13681 cgtcgtcccc ccgtcgggga ccatgaccgt cgtcttggcg ctctgggtcg tgatcgaggg
    13741 aatcccgccg gccgtcgtgc tgaaatcggc cgcgttgttc tggatgtcga tggtcatgat
    13801 gatcgtcccg tcggccgtga tctgaggggt ggcccgcagc tccaagccgg cgttgacgta
    13861 ttgagtggtc acggtgaagt tggctg
//
LOCUS       DAOOXH010000064        13566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_4601_length_13566_cov_7.530309, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000064 DAOOXH010000000
VERSION     DAOOXH010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 13566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13566
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4601_length_13566_cov_7.530309"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="PKV64_08080"
     CDS             <1..875
                     /locus_tag="PKV64_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17073.1"
                     /translation="LPAVAEADDRAYRSPQSLVERLKEGPFFDHLDVKLKIPPGASSV
                     KLLLRLRNTLLSTLLFYDLVLGSQGLDALSWIQRMNGDPAYAGRFWFLFRVFSGVRVK
                     VLTDSGWRPAGLIVDPGPLAFKRLALSIPASGREDIELRLEFVPDNYLIDSVNYDYEE
                     NPAADPAVIPLEFDDVRDMDGRPRPDVRALVADKDDRYLETEPGQAYRFFFNPPAARS
                     EEEQVSIFIASRGFYNEWLRGKWLEPPAGSDYRFDLGDVPGTLRRLAESWLESKTFLE
                     ERFFQTRIPIRGGW"
     gene            875..1477
                     /locus_tag="PKV64_08085"
     CDS             875..1477
                     /locus_tag="PKV64_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17074.1"
                     /translation="MIKLRWMGGTAFLLLAASCAVPLAEYEKNHYLLSPAGERIEIKD
                     KAGGRAAGEFLFADEDSVYVLIDGQKDLPPRVAAMPYKTIRTLKIRGVKNDKWLSAVL
                     LFQGLPALLLGATYMVDTDNPGGGILLTALTAVPAAVTALFLGTGPPAISLGRGGVDR
                     EKMRGFLKYARYPFIPDAVLKQKILDGLKRPAGPRTAESP"
     gene            1556..1996
                     /locus_tag="PKV64_08090"
     CDS             1556..1996
                     /locus_tag="PKV64_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17075.1"
                     /translation="MELKLTKSQYETLMMLVYLGNTVVNGFREKNPVMETDHLENYVY
                     AKAADFGLGKITIYDEELDEFYPSAETEDSWLKIHDDYINDLFWDELMHRLAERDLEN
                     RYGRDAVEAMPAEERGRLERELMDKLYAEFHQNGLNNLILYRPS"
     gene            complement(2043..3431)
                     /locus_tag="PKV64_08095"
     CDS             complement(2043..3431)
                     /locus_tag="PKV64_08095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN17076.1"
                     /translation="MSDAPAPAGKARFPRSFWTANTIELFERAAYYSMASFMVIYLTE
                     TLGMSPAAATFLNGTVLWGLIYFLPIVSGTIADKYGYKKSLSVSLVLITIGYLLMGNL
                     QGFWPGLTGAAAGEPVNYLLPVVLAVVFIGIGGSIVKPCIAGTVQKTSGRHATLAFGI
                     FYMVINIGSITGRGVSYFVRTSHGIPAIFTYVATVFALLGLAVVLFLYKEPQFVSDGI
                     KDGQVVKSRSLGEALLGIVTVLKNARFVFFLIVLSLFWFLYVQLYNLMPLFMRFIDPN
                     APMELYTMANPVMIVCFQLLITKLAKKWTPIRSIMFGVAVTTVGMAVNVLPTLLWSDV
                     TQKIDLLGLAIPAAGIFMIVSIASMATGEMFASPRIYEYLGALAPKGQEGLYLGYANL
                     PVALGSIVGGPVGGALFQKLILDPRNAGGPAKIATLWLIVGGVGICSFVGLGIYNAWL
                     KRALKKDAPAGA"
     gene            complement(3538..>3723)
                     /locus_tag="PKV64_08100"
     CDS             complement(3538..>3723)
                     /locus_tag="PKV64_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018159778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-pyrroline-5-carboxylate dehydrogenase"
                     /protein_id="HPN17077.1"
                     /translation="YYNDKCSGAMVGLQPFGGSRASGTNDKAGGSHNLMRWISPRTIK
                     ENYLPPADFPYPYMRAR"
     assembly_gap    3724..3823
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3824..5230)
                     /gene="pruA"
                     /locus_tag="PKV64_08105"
     CDS             complement(<3824..5230)
                     /gene="pruA"
                     /locus_tag="PKV64_08105"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HPN17078.1"
                     /translation="MNNAIVNFSIPRNEPVLDYLDGSPERKALVKELERQVSTVVDIP
                     AIIDGREVRTGETRDVVMPCDHGHVLARFHAVGPAEVQQAVEAACRAREHWMNLSWLE
                     RAAVTLKAARLISEKYRYTINAATMLGQGKNIFQAEIDSCCETVDFLRFNSYFISEIY
                     NEQPLSTPEQMNRMEYRPLEGFVFTVTPFNFTAIGSNLNMTPVLMGNTTVWKPATTAV
                     LSNYYLMQVYREAGLPDGVVNFLPGSGAVISGVTLGSKDLAGIHFTGSNSTFNGLWKT
                     VSGNLERYRSYPKLIGETGGKDFIFIHHSADTDQAATALIRGAFEYQGQKCSACSRAY
                     IPLSLWPEIKEKMLGMLSRIKVGDVRDFDCFVNAVIDEKAFDSIMGYISLAKKTPRAK
                     IIAGGEGDKSTGYFIQPTIIETDDPHLTTMEEEIFGPVLTVYPYPDDRYEDTLKICDE
                     TSPYALTGSIFARDRAALV"
     regulatory      complement(5319..5459)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            5624..6040
                     /gene="nikR"
                     /locus_tag="PKV64_08110"
     CDS             5624..6040
                     /gene="nikR"
                     /locus_tag="PKV64_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HPN17079.1"
                     /translation="MSDLIRFGVSLEKGLLAKFDKSIRKRGWASRSEALRDLIRAELV
                     RREWEEGEEVAGAVTLIYDHHRRDMLERLTDLQHDYHRQVLSTLHVHLDHDHCLEIIA
                     VKGKPSEIQKLADTLRTIPGVKHGTLTMSATGRNLG"
     gene            complement(6044..6346)
                     /locus_tag="PKV64_08115"
     CDS             complement(6044..6346)
                     /locus_tag="PKV64_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPN17080.1"
                     /translation="MEHHRRAASCLAHIMADMTAPFHMMGMPGAEAKKILDPGGTGKV
                     TGKTLIDEDIAGPGGFRIRDLDSTNGTYVRGERVSEAALCAGDEIMAGATRIGLGE"
     gene            complement(6482..8623)
                     /gene="glgP"
                     /locus_tag="PKV64_08120"
     CDS             complement(6482..8623)
                     /gene="glgP"
                     /locus_tag="PKV64_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPN17081.1"
                     /translation="MFVNHSNGHTRTSILAAGLNRLSRNLWWTWNQDAQAVFEELSPH
                     AWRYFQHNPVEVMREVSPAELRARLQDPLFESRVRAVLNAFELYMLEERTWGNANAAP
                     LRDRPVAYFSAEYGFHESLPIAAGGLGILSADHAKSASDLGIGYVGLGLFYREGYFRQ
                     SFNHENWQVESHSILDPLHLPLDPVLDESGHPIVPSLALAPGLVRFRAWRLNVGRIPV
                     YLIDSDLPENDTIVRDLTRHVYGGDNTNRILQELLLGIGGVRLLRILGLDPSVFHMNE
                     GHAAFLALELIREKMAAGRSFEDALAATRQECVFTTHTSVEAGHDRFSVDFLEYAAAK
                     YLQGFKQNRKQVIGLGRINPDNDAEPFCLTVLALKTTRAANGVSELHGGVSRSMWRPL
                     WPDRAEEDVPIGHITNGVHLLSWMTPKTWRFWKRRGGNDWPEQADAPSFWAKAEDAAF
                     VSDEELWAFRTVLRRDLIEFCRHRLYIQNRRFGPGEYLPSDLTLNPEALTIGFGRRAA
                     SYKRMTLLFDDLDAIVGMAKDALHPVQFVFSGKAHPRDEEGKRLIQKIIHLSKHSPLA
                     GSLVFLENYDIEVARAMISGCDVWLNNPRRPLEASGTSGMKSAAHGALNLSILDGWWR
                     EAYDGGNGFAIGADSHPADPAEQDRWDAANLLRVLTEEVVPTFYDRDSAGLPRRWIKM
                     IRRAMAGLSPRFNTRRMVQEYVQKIYLPRTP"
     gene            8868..9317
                     /locus_tag="PKV64_08125"
     CDS             8868..9317
                     /locus_tag="PKV64_08125"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPN17082.1"
                     /translation="MRIAVQDARRGFRRGDGGPFGAVVVIDGRIVGRGWNRVLVTRDP
                     TAHAEIVAIRRASRRLGRYDLSDAVLYATCEPCPMCLAAIHWARIRTCYFGCTTRDAA
                     RIGFDDKMIYESCGRLVRSWVTSVPFLREECLPLFEEWRGRSDRVVY"
     gene            complement(9336..10913)
                     /locus_tag="PKV64_08130"
     CDS             complement(9336..10913)
                     /locus_tag="PKV64_08130"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPN17083.1"
                     /translation="MTIRSKLAFVLGVSMLLFIALIQVFGRRVMKEQVRVQEESTVRT
                     DIKRVITLFEEEENGFVAFTESESFRADAAEILDPARNRSLDSHKVASVFLINQCDII
                     ACADPSGRVLQARMFDRKSGGERAVPTDFQARLDMIKPLRETLSPAEDFSVILAFEKI
                     PHLVVVHPSLKHPPNAFGDGLLIVARKIDGTFMEQLGRIANLPLRRLPTMKIGLPDGV
                     KAALDAGTNRFTTAVRAVSEDTISGLVQVRDPGGAPALVMSFDAPRLLNALFNRSWTL
                     FAGVFVGAVLLMMILSAVVIDKMVVARLSRLAAVVKTIDSDTDRPGPVPVEGGDEISG
                     LAVSINGMLESLHDYNEERRDLIQRLESDSLEDPLTGLYNRRGFLTIGREYLNLSARN
                     KTRMHLLFLDMDNLKQINDTFGHATGDEAIVRAAEIIKSVFRSSDVKSRLGGDEFAVF
                     PIASSIQGLKSSLSRFHEKISEFNRSGICPFTLSFSSGVAGYDPENPSTIEDLLARAD
                     KRMYEEKRRKTENDDRA"
     gene            complement(11104..11358)
                     /locus_tag="PKV64_08135"
     CDS             complement(11104..11358)
                     /locus_tag="PKV64_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17084.1"
                     /translation="MKACQDKWDALIGEIRANLNADPAGLVARDLARRRQALFNEACG
                     GHPGLGRKIDRACGGHGKGAMISADRCLSSRRSGISSRTP"
     gene            complement(11519..12403)
                     /locus_tag="PKV64_08140"
     CDS             complement(11519..12403)
                     /locus_tag="PKV64_08140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN17085.1"
                     /translation="MNKRLMIALAAVFCLAAALLIYFGGRPSAGGPAVGTVQEESIPS
                     FLGVGPHEITIRNVTKKPVHYRLRAYGSEAPAVERMLLPDEIDRYPAPQTVVISYLKY
                     GSEVTYSLFPGKPYSFRYDSAGNTDIWVGAHGREDAVDLAPYVPTPPEVLAEMCLLGE
                     VTKDSIVYDIGCGDGRIVIHAAKTFGARGVGIDIEPERIRESKANAKAAGVERLVRFK
                     LADATKVDISEATVVTMYLLPESNELLRPKLEKELRPGVLVVTHNYMIPGWENKEVTS
                     KIVLDSEEKEHTIFVYRR"
     gene            12553..13476
                     /locus_tag="PKV64_08145"
     CDS             12553..13476
                     /locus_tag="PKV64_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014657546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HPN17086.1"
                     /translation="MVNQDHLRNQKWRLGIIRHVEEVTHNVSKTCRYFGISRNAFYKW
                     QRRYVELGEEGLRDRSRRPIHSPRATKPDILAKIIYLRQTYHFGPWKIMVYLERYHDI
                     HISSSGIWRILKKLQMNRLPVNQRYQRHQERWKRYEKPMPGHRIQVDVKFLERIQESR
                     KRYYQYTAIDDCTRLRVLKIYDKNNQKTAIQFIDYTLSRLPFRTEVIQTDNGSEFQGQ
                     FHWHVLDKGINHVYIKPRRPRLNGKVERSHRTDEEEFYRMLNGVVIDDTQLFNKKLRE
                     WEDFYNYHRPHAALAGMTPYERFREKAGLQV"
BASE COUNT         2603 a         3953 c         4313 g         2597 t
ORIGIN      
        1 tcctccccgc ggtcgccgaa gccgatgatc gcgcctaccg cagccctcaa tcccttgtcg
       61 agcgcctgaa ggaaggaccc ttcttcgatc atctggacgt gaaattgaaa atcccccccg
      121 gggcgtcctc cgtgaagctt ctcctccgcc tgcggaacac gcttttgagc acgctcctgt
      181 tctatgacct ggtcttggga tcgcagggac tggacgcgct ctcctggatc cagcggatga
      241 acggcgatcc ggcttatgcc gggcggtttt ggtttctctt tcgcgttttt tccggggtcc
      301 gggtcaaggt cctgacggat tcaggctggc ggccggccgg cttgatcgtc gatcccggcc
      361 ccctcgcctt caaacgcctc gccttgagca tcccggcgtc cggacgggag gacatcgagc
      421 tccggctgga atttgttccg gacaattacc tgatcgactc cgtcaattac gattacgagg
      481 agaaccccgc cgcggatccg gccgtgatcc ccctcgaatt cgacgatgtc cgggacatgg
      541 acggccggcc gaggcccgac gtccgggcgc tcgtcgccga caaggacgat cgctatctgg
      601 aaacggagcc gggccaggcc tatcgtttct tcttcaatcc gcccgcggcc cgctcggagg
      661 aggagcaggt ttcgatcttc atcgcttccc gcggcttcta caacgaatgg ctccggggaa
      721 agtggctgga acctccggcc ggttccgatt accggttcga cctcggcgac gttccgggaa
      781 cgctccgccg gctggccgaa agctggctgg agtcgaagac ttttctcgag gaaaggttct
      841 tccagacccg gattccgatc aggggcggat ggtgatgatc aagctccggt ggatgggagg
      901 gacggcgttt cttctcttgg cggcgtcatg cgccgtcccg ctggccgagt atgagaagaa
      961 ccattatctc ctcagtcccg ccggcgagcg gatcgagatc aaggacaagg cgggcggaag
     1021 ggccgcgggg gaatttctct tcgccgacga ggattcggtt tacgttctga tcgacgggca
     1081 aaaagatctg ccgccgaggg tcgcggccat gccctataaa acgattcgaa cgctgaagat
     1141 caggggcgtc aagaacgaca agtggctgtc ggccgttctg ctctttcaag gcctgccggc
     1201 tcttctgctc ggcgcgactt atatggtgga tacggataat cccggcgggg ggatccttct
     1261 gaccgccctc acggcggttc cagcggccgt gacggccttg tttcttggga cggggcctcc
     1321 cgcgatctcc cttggaaggg gcggcgtcga tagggaaaaa atgaggggtt ttttgaaata
     1381 cgcccggtat cccttcatcc cggacgccgt cctcaagcag aagatcctag acggcctcaa
     1441 gcgtcccgcc ggtccgagga ccgcggaatc cccctgagcc tcgtcccggc cgccgcccgc
     1501 ccgcgttgtg ctataatgat cctcttcatt tttcgcgcgt tcacgaggat cgtccatgga
     1561 actcaaactg accaaatcgc aatatgaaac cttgatgatg ctggtttacc tcggcaacac
     1621 ggtggtcaac ggattccggg aaaagaaccc ggtgatggaa accgaccact tggagaacta
     1681 cgtctacgcc aaggcggcgg atttcggcct gggcaagatc acgatttacg acgaggagct
     1741 cgacgagttt tacccgtcgg ccgagaccga ggactcctgg ctgaaaatcc acgatgatta
     1801 catcaacgac ctgttctggg acgagctcat gcaccgcctg gccgagcggg acctggaaaa
     1861 ccggtacggc cgggacgccg tcgaggcgat gccggcggag gaacggggcc gcctggagcg
     1921 ggaactgatg gacaagcttt acgccgaatt ccaccagaac ggattgaata acctcattct
     1981 ctaccggccg tcctgaagcc cggccccggc cgtccgcggc ctccgccggg ggagaagaac
     2041 cgtcaggcgc cggccggcgc gtccttcttc aaagcccgct tgagccaggc gttgtagatc
     2101 cccagcccga cgaacgagca gattcccacg ccgccgacga tcagccagag cgtcgcgatc
     2161 ttcgccggtc cgccggcgtt ccgcggatcg aggatcagct tctggaacag ggctccgccg
     2221 accggccctc cgacgatgct gcccagggcg accggcaggt tggcgtagcc gaggtagaga
     2281 ccctcctggc ccttgggggc gagggcgccc aggtattcgt agatccgcgg ggaggcgaac
     2341 atctcgccgg tggccatcga ggcgatcgag acgatcatga agatcccggc cgccgggatc
     2401 gccaagccga gcaggtcgat tttttgggtc acgtccgacc agagcagggt cggcaggacg
     2461 ttgacggcca tgccgacggt cgtcacggcc actccgaaca tgatcgaccg gatcggcgtc
     2521 cactttttag ccagcttggt gatcaggagc tggaagcaga cgatcatcac cgggttggcc
     2581 atcgtgtaca gctccatcgg ggcgttggga tcgatgaagc gcatgaagag gggcatgagg
     2641 ttgtagagct ggacgtaaag gaaccagaag agggacagga cgatgaggaa aaagacgaaa
     2701 cgggcgttct tcaggacggt gacgatgccc aggagggctt cgcccaagga gcggctcttg
     2761 acgacctggc cgtccttgat cccgtcgctg acgaactggg gctccttgta gaggaacaag
     2821 acgacggcca atccgagcag ggcgaagacc gtcgccacgt aggtgaagat ggccggaatg
     2881 ccgtggctcg tccggacgaa ataggagacg ccccgcccgg tgatcgagcc gatgttgatg
     2941 accatgtaaa agatgccgaa ggccagggtc gcgtgccttc ccgaggtctt ctggaccgtc
     3001 ccggcgatgc agggcttgac gatggagccg ccgattccga tgaatacgac ggcaaggacg
     3061 acggggagga gatagttgac cggctcgccg gcggccgccc cggtcaggcc cggccagaat
     3121 ccctggaggt tgcccatcag caggtagccg atggtgatta aaacgaggga aaccgagagg
     3181 gatttcttgt agccgtactt gtcggcgatc gtcccggaga cgatcgggag gaaatagatg
     3241 agcccccaaa ggaccgtccc gttgaggaag gtggccgcgg ccgggctcat ccccagggtt
     3301 tccgtgaggt agatgaccat gaacgaggcc atcgagtagt aggcggcccg ttcgaaaagc
     3361 tcgatggtgt tggccgtcca gaagctgcgg ggaaaccggg ccttgccggc cggggcgggg
     3421 gcatcgctca tgggaacact cctttacgga tttcgacagt ccaaacgaag aaggggaatc
     3481 atatcgaagc cctccgccgc ggtcaaccgg gaccgcggcg gagggcggcg gcccggctta
     3541 acgggcccgc atgtaaggat agggaaaatc ggccggcggc aggtagtttt ccttgatcgt
     3601 ccggggggag atccagcgca tcaggttgtg gcttcccccg gctttgtcgt tcgttccgga
     3661 cgcccgggat ccgccgaagg gttgaagacc gaccatggcc ccggagcatt tgtcgttgta
     3721 gtannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngaccagg gcggcccggt
     3841 cgcgggcgaa gatggatccg gtcagggcat agggagacgt ctcatcgcag attttcaagg
     3901 tgtcttcgta gcgatcgtcc ggataggggt agaccgtcag gacggggccg aagatttcct
     3961 cttccatggt cgtcagatgg ggatcgtcgg tctcgatgat cgtcggctgg atgaaatatc
     4021 ccgtgctctt gtcgccctcg ccgccggcga tgatcttcgc ccggggggtt ttcttggcca
     4081 gggagatgta tcccatgatc gaatcgaagg ctttttcgtc gatcaccgcg ttgacgaagc
     4141 aatcgaaatc gcggacgtcg ccgaccttga tccggctcag catgcccagc atcttctcct
     4201 tgatttcggg ccaaagggac aagggaatgt acgcccggga acaggcggag catttttgcc
     4261 cctggtattc gaacgccccc cggatcaagg ccgtcgcagc ctgatcagta tcggccgaat
     4321 ggtggatgaa aatgaaatcc tttccgcccg tctcgccgat gagcttgggg taggaccggt
     4381 agcgctcgag gttcccggag acggtcttcc acaatccgtt gaaggtggag ttggatccgg
     4441 tgaagtgaat gccggccaag tccttcgagc ccaaggtcac gccgctgatg accgcgccgg
     4501 aaccggggag gaagttcacc acgccgtccg gcaggccggc ttcccggtag acctgcatca
     4561 ggtaataatt ggacagaacc gcggtggtgg ccggcttcca aacggtcgtg ttgcccatca
     4621 ggaccggggt catgttgagg ttcgagccga tggccgtgaa attgaagggc gtgaccgtga
     4681 agacgaagcc ttccagcggc cggtactcca tccggttcat ctgttccggc gtggacaggg
     4741 gttgttcgtt gtagatttcc gagatgaaat aggaattgaa ccggagaaag tccacggtct
     4801 cgcagcagga atcgatttca gcttggaaga tgttctttcc ctgccccagc atggtcgcgg
     4861 cgttgatcgt gtagcggtat ttttcggaaa tcaaccgggc ggccttcagg gtgacggccg
     4921 cccgctcgag ccaggataga ttcatccagt gttccctggc ccggcaggcg gcctcgaccg
     4981 cctgttgcac ctcggccggc ccgaccgcat gaaaccgggc caagacatgg ccgtgatcac
     5041 agggcatcac gacgtcgcgt gtttcgccgg tccggacttc ccggccgtcg atgatggccg
     5101 ggatatcgac cacggtggag acctggcgtt caagctcctt gaccagcgcc ttccgttccg
     5161 gcgagccgtc caggtaatcc aagaccggct cgtttcgggg aatggagaaa ttaacgatcg
     5221 cgttgttcaa tacaccctct ccttattgaa ttatgatccc cgatcagaaa aaaaaagacc
     5281 cttttcccgg agagaaaagg gtcttgcgga cgccgaggcg gcgtccctct ttctctcctt
     5341 tccaaacacg ggaggccgag ccgtttccag cccgaccctt gtccgccgcg gagcggaacc
     5401 gtcccgcctt cgtgacaacg tcgtgtttta gaaggacggg atcggagatc gaatccattc
     5461 ttaccgatta aacggaggtt gtcaatcctt tgaaggcccc atgacgaatt gcgaggtgaa
     5521 aaaaggggaa gtgcgggaag attttcaatg gcgcgattcg cccccttttt ttgggaggaa
     5581 tctcgtgtta cgatcaaaaa gagcgtgctc ccggaggacg aacatgagcg atcttatccg
     5641 tttcggcgtg tcgctggaga agggacttct ggccaagttc gacaaatcga tccgcaagcg
     5701 cgggtgggcc agccggtccg aggctctgcg cgacctgatc cgggccgagc tcgtccggcg
     5761 ggaatgggag gaaggggagg aggtcgccgg agccgtgacc ctcatttacg accatcaccg
     5821 ccgggacatg ctcgagcggc tgaccgacct tcagcacgac taccaccgtc aggttctatc
     5881 caccctccat gtccacttgg atcacgacca ttgcctggaa atcatcgccg tcaagggaaa
     5941 gccgtccgaa atccagaagc tggccgacac ccttcggacg attccgggcg tcaagcacgg
     6001 cacgctgacg atgtcggcga ccgggcggaa tctcggctga gattcactcc ccgagcccga
     6061 tccgggtcgc cccggccatg atctcatcgc cggcgcagag cgccgcctcc gaaacccgct
     6121 cgccccggac atacgtcccg ttggtgctgt cgagatcgcg gatccggaag ccgccggggc
     6181 cggcgatatc ctcgtcgatc agcgtcttgc cggtgacttt tcccgtcccg cccggatcta
     6241 gaatcttttt cgcctcggcg ccgggcatgc ccatcatgtg aaaaggagcg gtcatgtcgg
     6301 ccatgatatg ggcgaggcag gaggccgcac ggcgatggtg ttccatgctc ccttcctcct
     6361 gtggacggga aaataatccc gccggcgagc gcgtgtcaat ccgctcttct tgatcgccgc
     6421 gccggttatg tcgaggacga tccgcgggcc ggagtccgga tttccgtccg tcttttaaag
     6481 cttaaggcgt tcgggggagg tagattttct ggacatattc ctggaccatc cggcgggtgt
     6541 tgaaccgggg actcaggccg gccatcgccc gccggatcat tttgatccag cggcggggaa
     6601 ggccggcgga atcccggtca tagaacgtcg ggacgacctc ctcggtcaat acccggagaa
     6661 ggttcgcggc gtcccagcgg tcctgctcgg cgggatcggc gggatgggaa tcggcgccga
     6721 tggcgaagcc gttccctccg tcgtaggctt cccgccacca gccgtccagg atgctcaggt
     6781 tcagggcgcc gtgggcggcg cttttcatcc cgctcgtccc ggaagcctcc agcggccggc
     6841 gggggttgtt gagccagaca tcgcagccgg agatcatcgc ccgggcgact tcaatgtcat
     6901 agttttccag gaaaaccagg gacccggcca gcggcgaatg cttgctgagg tggatgatct
     6961 tctggatgag gcgcttgccc tcctcgtcgc ggggatgagc tttgccggaa aaaacgaatt
     7021 ggaccggatg cagagcgtct ttggccatcc cgacgatcgc gtccaggtcg tcaaacagga
     7081 gggtcatgcg cttgtaggag gcggcccggc ggccgaaacc gatcgtcagg gcttccgggt
     7141 tgagagtcag gtcggaggga aggtattctc ccggtccaaa ccggcggttt tgaatgtaga
     7201 gacggtgccg gcagaattca atcaggtcgc gccgcaggac ggtccggaag gcccacagct
     7261 cctcgtcgga cacgaaggcg gcgtcttcgg ctttcgccca gaacgatggg gcgtcggcct
     7321 gctccggcca atcgtttccg ccccggcgct tccagaaccg ccatgttttc ggcgtcatcc
     7381 aggacaggag atggacgccg ttggtgatgt ggccgatggg gacgtcctcc tccgcccggt
     7441 cgggccacag gggccgccac atggaccggc tgactccgcc gtgaagctcg ctgacgccgt
     7501 tggccgcccg ggtggttttt aatgccagga ccgtcaagca gaacggctcg gcgtcattgt
     7561 ccggattgat ccgtcccagg ccgatgacct gcttccggtt ctgcttgaat ccctggaggt
     7621 attttgcggc cgcgtattcc aggaaatcca cgctgaagcg gtcgtgtccc gcctccaccg
     7681 aggtgtgggt cgtgaagacg cattcctgcc gggtggcggc cagggcgtcc tcaaaggatc
     7741 ggccggcggc catcttttcc cggatgagct ccagcgccag gaaagccgcg tgtccttcgt
     7801 tcatgtgaaa aacggaggga tcgagtccca agatccgcag caggcgcacg ccgccgatgc
     7861 ccagcagaag ctcctggaga atccggttcg tgttgtcccc gccgtagaca tggcgggtca
     7921 ggtcgcggac gatcgtgtcg ttttccggca ggtcggagtc gatcagatag acggggatgc
     7981 ggccgacatt gaggcgccag gcccggaacc tgacgaggcc gggagccagg gccagggagg
     8041 gaacgatggg atgcccgctt tcatcgagga cgggatccag cgggagatga agaggatcga
     8101 ggatggagtg ggactcaacc tgccaatttt catgattgaa ggattggcgg aaatagccct
     8161 cccggtaaaa gagcccgagg ccgacgtatc cgattccgag gtcggaggcc gatttcgcgt
     8221 gatcggccga gaggatgccc agtcccccgg cggcgatggg gagggattca tggaagccgt
     8281 attcggcgct gaaataggcg acgggccggt cgcggagggg ggcggcgttg gcgtttcccc
     8341 acgtccgttc ctcaagcatg taaagctcga acgcattcaa gacggcccgg acccgggatt
     8401 cgaaaagggg atcctggagg cgggcccgga gctcggccgg ggagacctcg cgcatgacct
     8461 cgaccggatt gtgctggaaa tacctccagg cgtgggggga cagctcctcg aaaaccgcct
     8521 gggcgtcttg gttccaggtc caccatagat tgcgggacag ccggttcagg ccggccgcca
     8581 ggatcgatgt ccgcgtgtgg ccgtttgaat gattcacaaa catcccgttc tcccggggcg
     8641 cttcgcccgc gccgaaaaac ggcgactatc atagcccgtc cccccgttcc tttcaagggg
     8701 atccggttgc gcccgccggg attccggttg ttttccgacg ggggggaata tggtacaaag
     8761 gggacgtgaa aatccaaaaa acgcgtccaa gcaaatccgc ggatgcttcc ccccgcgccg
     8821 gccgggggac ggcgcggaag ccccaggccg ggcccgacaa gcgctttatg agaatcgccg
     8881 tccaggacgc ccggcggggc tttcggcggg gcgacggggg accgttcggc gccgtcgtcg
     8941 tcatcgacgg tcggatcgtc ggccgggggt ggaatcgcgt tctcgtcacc cgcgacccca
     9001 cggctcacgc cgagatcgtg gccatccgcc gggcgtcgcg gcggctcggc cgctatgatc
     9061 tttccgacgc cgtcctttac gcgacctgcg aaccctgccc gatgtgcctg gcggcgattc
     9121 attgggcccg gatccggacg tgttatttcg gctgcacgac ccgggacgcc gcccgaatcg
     9181 gtttcgacga caagatgatc tatgaatcct gcggccggct cgtccgttct tgggtcacgt
     9241 ccgtcccgtt tctccgggag gagtgcctgc cgctgttcga ggaatggagg gggcgttccg
     9301 accgggtcgt ctattgaggg gctgcatttc cgggctcagg cccggtcgtc gttttccgtt
     9361 ttccggcgct tttcctcgta catccgcttg tccgcccggg ccaacaaatc ctcgatggtc
     9421 gaaggatttt cggggtcata cccggccacc ccggagctga aggacagggt gaacgggcag
     9481 atcccggagc ggttgaactc cgagattttt tcgtgaaaac gactgaggga agacttcagg
     9541 ccttggatgg acgaggcgat cggaaagacg gcgaattcat cgcctccgag gcggcttttc
     9601 acgtcggagc tccggaaaac cgatttgatg atctcggccg cgcggacgat ggcctcgtcg
     9661 cccgtggcat ggccgaacgt gtcgttgatc tgcttgaggt tgtccatgtc caggaagagc
     9721 aagtgcatcc gggttttatt ccgggcggag agattgaggt attcccggcc gatggtcagg
     9781 aatccccgcc ggttgtacag ccccgtcagg gggtcttcca gggaatccga ctcgaggcgt
     9841 tggatgagat cgcgcctctc ctcgttgtaa tcgtgaaggg attcgagcat tccgttaatg
     9901 gaaacggcca atccggaaat ttcgtctccg ccctccaccg ggacaggacc gggacggtcg
     9961 gtgtcggaat cgatcgtctt gacgacggcc gccagccggg acaggcgggc cacgaccatt
    10021 ttatcgatga ccacggcgct gaggatcatc atcagcaaga cggcccccac gaatactccg
    10081 gcaaagaggg tccaactccg gttgaatagg gcgttaagaa ggcggggcgc atcgaaggac
    10141 atcaccaggg ccggagcgcc gccgggatcc cggacctgca caaggcctga gatcgtgtcc
    10201 tcggagacgg cccgaacggc cgtcgtgaac ctgttcgtgc cggcgtccag ggcggccttg
    10261 acgccgtcgg gcaggccgat cttcatggtc ggcaggcgcc tgagagggag attcgcgatc
    10321 cggccgagct gttccatgaa cgtgccgtcg atcttccggg cgacgatcag aagcccgtcg
    10381 ccgaaggcgt tcggcgggtg tttcagggaa gggtggacga cgacgagatg cgggatcttc
    10441 tcgaaggcga gaatcacgct gaaatcctcg gccggggaca gggtttcccg cagcggcttg
    10501 atcatgtcaa gccgcgcttg aaaatcggtt gggacagccc gctctccgcc ggatttccgg
    10561 tcgaacatcc gggcctggag gacgcgtccg gacggatccg cgcaggcgat gatgtcgcac
    10621 tggttgatga ggaaaaccga agcgaccttg tgggagtcca ggcttctatt ccgggcggga
    10681 tcgaggattt ccgcggcgtc ggcccggaag gattcggact cggtgaaggc gacgaagccg
    10741 ttctcctcct cctcgaaaag cgtgatgacg cgtttgatgt ccgtccgcac cgtggattcc
    10801 tcttggacac ggacctgttc tttcatcacg cggcgcccga aaacttgaat cagggcgatg
    10861 aaaagcagca tcgagacgcc caggacaaag gccagcttgc tgcggatggt catgaatgcg
    10921 cgtctttcag ttcccaggac ggacggaccg aaccgcccct ttccggcccg ggccctcctt
    10981 aaattaagct cgaaccgggc ttcttgtcaa ccggcgggcc gggccgggac actcgggccg
    11041 cccatcgttc cgcgtcccga agagctcgga gggaggacgg cccgcagggt attccggcgg
    11101 ttttcagggc gttcttgatg aaatcccgga tctccggctc gaaaggcatc tgtccgccga
    11161 aatcatcgcc ccttttccat gcccgccgca cgcccggtcg atctttcgcc caagcccggg
    11221 atgcccgccg caggcttcgt taaacagcgc ctgccggcgc cgggccaagt cccgggcgac
    11281 gagacccgcc ggatcggcgt tcaagttcgc ccggatttcg ccgatcaggg cgtcccactt
    11341 gtcctggcag gctttcatag cctgaggcga ataggatttc tcgatgccct cgaattcctt
    11401 caggtcgtct tcggtgaaga acttctaggt ccattcccgc ttgttttgcc cgctcgtccg
    11461 cgatttctcc atgattccga tgatccggct ccggccgatc cggccgcggc gacgagggtc
    11521 agcgccggta aacgaaaatc gtgtgttctt tctcctcgga atccaggacg atcttggagg
    11581 tgacttcctt gttctcccag ccggggatca tgtaattgtg ggtaaccacg aggacgcccg
    11641 gccggagctc cttctcgagc ttgggccgga ggagctcgtt ggattcgggc agaaggtaca
    11701 tcgtcacgac cgtggcctcg gagatgtcca ccttggtcgc atcggccagt ttgaaccgga
    11761 ccagccgttc cacgccggcg gctttcgcgt tggccttgga ctcccggatc cgctcgggct
    11821 cgatgtcgat gccgacgccc cgggcgccga aggtcttggc cgcgtgaatg acgatccggc
    11881 cgtcgccgca gccgatgtcg tagacgatcg aatccttggt cacctctccc aagaggcaca
    11941 tttctgccag gacctcgggc ggcgtcggga catagggcgc caggtcgacg gcgtcctccc
    12001 ggccgtgggc gcccacccag atgtccgtgt ttcccgcgct gtcgtagcgg aaggagtagg
    12061 gcttcccggg gaagagggaa tacgtcacct cggatccgta cttcaggtag gaaatcacga
    12121 cggtttgagg cgccggatag cggtcgattt catccggcag gagcatccgc tccacggccg
    12181 gggcttccga gccgtaggcc cggagcctgt agtggacggg cttcttggtg acgttccgaa
    12241 tggtgatttc gtggggcccg actcccagga aggagggaat cgattcttcc tgaaccgtcc
    12301 cgaccgccgg gccgccggcc gagggtcgcc cgccgaaata aatcaggaga gccgcggcca
    12361 ggcagaagac ggccgccagg gcgatcatca ggcgcttgtt catcgcttca ccttccggcg
    12421 aggctccgcc cgccgggcct cattttgtcc caagagaggg ggcgggtcaa gggggtctaa
    12481 aactgactga catggtttac acctagggct ctatttggat aggcttaggg catttaggga
    12541 aggaggagcc ttatggtaaa ccaagatcat cttaggaatc agaagtggcg tttaggtatc
    12601 atccggcacg tcgaggaagt gacccataat gtttccaaga cctgcaggta cttcggaata
    12661 agccggaacg ccttctataa atggcagaga aggtatgttg aacttggtga agagggcctg
    12721 agagaccgtt cccgtcgacc gatccatagc ccccgggcta cgaagcctga catactagcc
    12781 aagatcatct atctgaggca gacctaccat tttggcccct ggaagattat ggtctatctc
    12841 gaacgatatc acgatatcca catcagcagc tccggcatct ggcggatcct taaaaagctc
    12901 cagatgaacc ggctgccggt caatcagcga tatcaaagac accaggaacg ttggaagagg
    12961 tacgagaagc ccatgcctgg gcataggatc caggtcgatg ttaagttctt agagaggatc
    13021 caagagagcc ggaagcgcta ttatcaatat acggcgatcg atgactgtac gaggcttcgg
    13081 gtcttgaaga tatatgacaa gaataaccag aaaaccgcga tccagttcat tgattatacg
    13141 ctctcccggc tgccttttag gacggaagtg atccaaaccg acaatggttc tgagtttcaa
    13201 gggcagttcc attggcatgt gctggacaag ggaattaatc acgtatatat aaaacctcga
    13261 cgaccaaggt taaatgggaa ggtcgagcga agtcacagaa ccgacgaaga ggagttttat
    13321 cggatgctaa acggggtcgt catcgacgac acgcaacttt ttaataagaa gctgagggaa
    13381 tgggaagact tctacaacta ccatcgacct cacgcggctc tagccggaat gaccccgtat
    13441 gagcgcttcc gggaaaaagc cgggcttcag gtgtaatcga tcatggtcag acccacaggg
    13501 ggcggcgcgc agttgacaaa accgctcggc cgccgtacta ttcagattgt aatgattaca
    13561 aattga
//
LOCUS       DAOOXH010000065        13543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_4625_length_13543_cov_7.059164, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000065 DAOOXH010000000
VERSION     DAOOXH010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 13543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13543
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4625_length_13543_cov_7.059164"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PKV64_08150"
     CDS             complement(<1..449)
                     /locus_tag="PKV64_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPN17087.1"
                     /translation="MKHRTLQNRLGAFADGELRGIKRRRLEKHLAACPACRTELEVIR
                     RLDAFARESAEAPVVPGYWESFPGRVAEGLRRRRREAGRPARNALFDDSFLVPESRLR
                     TKAWALPLSIVSHAAVALLLIILPLLQVDHLPQIEITSAFLAPPPPPP"
     gene            complement(446..946)
                     /locus_tag="PKV64_08155"
     CDS             complement(446..946)
                     /locus_tag="PKV64_08155"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPN17088.1"
                     /translation="MEAYENLVRTFERPIYFLCLRMAGAPQAADDLAQETFIRAYGAL
                     SGFKEGRDFYAWLRRIAVNASLNHLRAVHREEPLGDREPAPSRDTPQDELQRREAEGA
                     FQEALGALPFDQRAVFVLRVFEGRSYRDIARDLKISKGTVMSRLSRARAKLRFMLADR
                     VERRRS"
     gene            1200..2981
                     /locus_tag="PKV64_08160"
     CDS             1200..2981
                     /locus_tag="PKV64_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPN17089.1"
                     /translation="MIQTLGQIPLNTLRAYGKPDQLLYKADGKYVPISAQELYDRVRQ
                     ISLGLRSLGLGAGDRLVILSENRPDWVMTDFAVLAAGAVTVPIYPTLTPEQIRYIVDD
                     SEAKIVVCSTPDLQAKVDAIRPSLSLVAHFLSFEDQPAGGFIPFKDVVEKGRQAEAAD
                     PEAFERAAAAVKPDDIASIIYTSGTTGVPKGVMLSHDNFFSNTRALDAVTDFHCGDTI
                     LSFLPLSHVLERMTMFSFLYKGSTVAFAESIDTVAQNLLEVRPTIMVSVPRLFEKLYA
                     RIMDSILAGSALKKKIFFWALRGGLSASRKAQRRTAALFPRRSLRLRLAQKLVFSKIL
                     EKTGGRVKFFVSGGAPLSRDIAEFFHAIGLVILEGYGLTETSPVVACNTFTKIRFGTV
                     GPVVPKVEVQIAPDGEILVRGPNVMKGYFKKEAETAEALAGGWLHTGDIGHLDVDGFL
                     VITDRKKDLIVTAGGKNVAPQPIENLLKQSSYIAQTVVVGAGRKFISVLVVPNFEKLE
                     AWARERNIAFAARKDLCGRDDVRDFYREEIERCTPGLAPYEKIKKILLLEREFEIERD
                     ELTPTLKVKRSRVEEKYKALIDGLYRE"
     gene            2999..3769
                     /locus_tag="PKV64_08165"
     CDS             2999..3769
                     /locus_tag="PKV64_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17090.1"
                     /translation="MKIFRGMLPAVVAAATVSCVIPVYVDEGSRWSREPEAFQQVSEF
                     AAGGVLRVTNIYGNIIIRGWDREELEVTAEETRNDSAASIPRVEIASEGGETAITAGP
                     GDEDFAGGRTVHLLIRVPHRVFLRSVTAGRGRLQLSDLYGEAFLRLDDGDLRVENYSG
                     SLDAEIGRGSMEVELLDARPEDAVRLVLDEGPIDVLLEPGFEGRLEVEAPEGTVSCDF
                     EVTPAAEPARVSGTVGSGEGALVIVSTRHGSVDVRKTK"
     gene            3780..4589
                     /locus_tag="PKV64_08170"
     CDS             3780..4589
                     /locus_tag="PKV64_08170"
                     /inference="COORDINATES: protein motif:HMM:NF022665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HPN17091.1"
                     /translation="MEEKQKIIRAAVVVLIVVLLGLCAYYYFFSGRLKNADGGITPAQ
                     AEKIDAEKPREIDAAAAAPLAVPLDGSDEAVRALAGEISSDPVLARWLRSRDLIRRCA
                     AAVDAVANGQSPRTQFDFFIPGGPFKTYVRGGETYLDPSSYARYNTAADVFDSLGTAA
                     CARAYAQCRDLLQQAYRELGYPAGDFHQTLYRAIIEILRTPVLEQPIAVKKDITTYLF
                     KDEKLESLDDVQKHLLRMGPENLQLIQVKLREIALALGFTEAQLPVPRPYR"
     gene            complement(4601..5599)
                     /locus_tag="PKV64_08175"
     CDS             complement(4601..5599)
                     /locus_tag="PKV64_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPN17092.1"
                     /translation="MTKIERIEAALSAWKGREEACDLCPRRCRTDRRAGPSGFCRTGV
                     KAVVASSLLHFGEEPVLSGLSDGCVPETGRRRRLGSGTIFFCGCNLRCLFCQNYQISW
                     LGRGRPVEDEELAGLMLDLERQGALNINLVSPTQSLLPILRALRLALIRGLSLPLVYN
                     TNGYERADVLERLAGIIDIYLPDFKYALSEPAARLSGAADYFEWAGPALQEMFVQQPD
                     LELDEEGRAVRGTIVRHLVLPGQVPNSLAVLDWLAQTFRPTVPLSLMSQFRPCWRVPP
                     DLDRPLHPGEYEAVLDRARRLGFDPLFAQPELFADGRHLNPDFDRKDPFPWKRRNA"
     gene            complement(5618..6055)
                     /locus_tag="PKV64_08180"
     CDS             complement(5618..6055)
                     /locus_tag="PKV64_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17093.1"
                     /translation="MALKDKAIDPNLKPDPELAAAVPGLRESDGTVSCAAAMRLAETK
                     NTDPEAVGRTLDAMAVTLGRCQIGTFGYPGHAKGWTGAGLEARPVPDGLENALREAGG
                     NDRSITCSDIWALASRFGLSRLHAGWLADRAGLKIRKCQLGAF"
     regulatory      6157..6299
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            6291..7367
                     /locus_tag="PKV64_08185"
     CDS             6291..7367
                     /locus_tag="PKV64_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPN17094.1"
                     /translation="MGRIKFVFIVILILFAGTAFGAAPDGPLEFQDAAGRTIRLEKPP
                     ERILAIGHGPQIIAHLMFMFPESRSRFIGWERRGRIASEFIPLIDPDFDKRYFTDPNT
                     GVEEVAASHPDLVLIRGFVTDPKGDALEKVGIPVAYLGLENPEQYRKDIELAGLILGN
                     PGRAAEINRFFQERLDRIEHALAGLEEARKPSVFLAMTMSRGGKIAVRVPAPSWIQTV
                     MVRLAGGRPVWAEGMVDTSAWSVVNIEQIARWDPDGIILVVWHTLDPQKTLAALKTDE
                     TWRALRAVQGDKMRAFPADIYGWDTPDPRWILGLTWLARTFHPDRFPGLDMKAEIVSF
                     YRDLYGLSPDDVEAKILPSIRTGY"
     gene            7371..8378
                     /locus_tag="PKV64_08190"
     CDS             7371..8378
                     /locus_tag="PKV64_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPN17095.1"
                     /translation="MDSAADRRVRIRRIWLLAGLSAAALAVSVLLGRYPGPVLTPPSV
                     IGTDTLARDLVFHIRIPRVAAAFVLGAALSAAGAVFQTVFRNPLVDAGFLGVSQGAAF
                     GASAAIVFASGNAWAVQGGAALFAFLGLALSYALATRIRVGDEILHLVLAGIVVGAFY
                     AAGTGILKYIADPQKQLPDITYWMLGGLWGITGRDVLQMLPVAAAGLAYMLAVRWRLN
                     LLAMRDETAFSLGAAPGRERLFVLIAAVAVTAAGVAKAGQIAWVGLIVPHICRRLVGS
                     ETRVLLPASVLLGGIFVLVCDDVARVALSGEIPLGILTSLLGTLLFLVLLLQRKIRGV
                     G"
     assembly_gap    8548..8647
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8648..9019
                     /locus_tag="PKV64_08195"
     CDS             <8648..9019
                     /locus_tag="PKV64_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN17096.1"
                     /translation="AGKPVRTLSTGERQLASIARALAQDPGLLLLDEPTSHLDLANTR
                     RVLRILRFLRDRGKTVVLTTHDPNTASVTADRIVLLHEGRVAAAGTPAEVITGARLSG
                     VYGVEIDVRMIDGSPHVLAKL"
     gene            9328..9990
                     /locus_tag="PKV64_08200"
     CDS             9328..9990
                     /locus_tag="PKV64_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HPN17097.1"
                     /translation="MELISQAVDFVLHLDKHLNGLIQMFGGWTYGLLFVVIFCETGLV
                     VTPFLPGDSLIFAAGAFAAAGSLKIGWLFLILAAAAVLGDTANYWIGKIIGPKVFTKE
                     KSRIFRKEYLDRTHRFYEKYGAETIILARFVPIVRTFAPFVAGIGRMNYGKFLSYNVV
                     GGVAWVGLFSFGGYFFGNLSFVKKHFSLVIIAIILISLVPVLWEFLRHRRDKKKTEGT
                     AG"
     gene            complement(10000..10779)
                     /gene="surE"
                     /locus_tag="PKV64_08205"
     CDS             complement(10000..10779)
                     /gene="surE"
                     /locus_tag="PKV64_08205"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPN17098.1"
                     /translation="MTCAAEPLILLCNDDGAHRAEIRVLRRRLGALGQAVIVAPDRER
                     SAFSLALTLRHPLRLHRDGPGVWSVDGTPADCVYLAVDKVLPRRPDLLISGLNPGPNL
                     GQLDAHYSGTVAAAFQGTFFGIPSLAVSMIPDAAGRFYREDAADVVFRLAERILASGL
                     PDGVTLNVNIPAPPLRGLRITRLGRKFYERDVLEKKDPRGQPYFWIGTGKVLFDGSSE
                     TDIRTIERGYVSITPLRTDLSWIPDSGASPLRKIFRGMEWA"
     gene            complement(10826..10902)
                     /locus_tag="PKV64_08210"
     tRNA            complement(10826..10902)
                     /locus_tag="PKV64_08210"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10866..10868),aa:Pro,seq:ggg)
     gene            complement(11132..11509)
                     /locus_tag="PKV64_08215"
     CDS             complement(11132..11509)
                     /locus_tag="PKV64_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPN17099.1"
                     /translation="MSPKDEQKLFYRLAEVCRLAKIDAETIALWEKEFPFIEPGETAS
                     GRKIYRAKDLEIILRIKDLLGKEQLTLAGARRRLEAEMDLRPVPAVHPDKIVKLLWRV
                     RDELQDLSQSLGRPAKKERRPAD"
     gene            11637..12962
                     /locus_tag="PKV64_08220"
     CDS             11637..12962
                     /locus_tag="PKV64_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPN17100.1"
                     /translation="MPKAKPETLVPLAERMRPETFDRLYGQEHLLGKGKILTQLIESR
                     RLVSLIFWGPPGSGKTTLAMMLARHFDLPSTFFSAVLSGIKEVKEVMAQAESHKRLYG
                     KPTIIFIDEIHRFNKAQQGAFLPYVERGDIILFGSTTENPSFEVIAPLLSRTRVLTLE
                     PLDDEAVSRILDDALADPDRGIGRRGLSLDPAARRMLIDQSNGDARRSLNTLELAADL
                     AGSGVIGPDHVREAQQKRMLLYDKDGEEHYNLISALHKSLRNSDVDAALYWLARMLYA
                     GEDPLYIARRMVRFASEDVGLADPQALAVTLRAKEAYEFLGSPEGDLALAEAVVYLAA
                     APKSNRVYEAFGKVRKDVEERPHEPVPKVIRNAVTALMKEAGYGKGYAYAHDRPEGTT
                     DMETMPDRLAGRRYYDPGPFGYEKEIRKRMDWWAGVKARFRSEKENAGT"
     gene            complement(12963..13133)
                     /locus_tag="PKV64_08225"
     CDS             complement(12963..13133)
                     /locus_tag="PKV64_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17101.1"
                     /translation="MVNYILLYKIKKKVKAMIKDKLALGEIATTPSSCLDCLATDLSW
                     EIYYLMKEKSET"
     gene            13217..>13543
                     /locus_tag="PKV64_08230"
     CDS             13217..>13543
                     /locus_tag="PKV64_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17102.1"
                     /translation="MKTTRLFAVPAILALLSASRGIPRLNPEETAVLKFPLVEASRVT
                     CRGPLVPAIIPGPGGLLLVQSRTGFLQAVDPEKSVILWTVPVGSGALPPVVSGDRIVA
                     AWSNGRV"
BASE COUNT         2408 a         4248 c         4339 g         2448 t
ORIGIN      
        1 ggaggcggcg gcggaggcgc caggaacgcg ctcgtgatct cgatttgggg aagatgatcg
       61 acttggagga gaggcaggat gattaaaagc agggccacgg ccgcgtgaga gacgatcgac
      121 aggggcaggg cccaggcttt cgtccggagg cgggattccg ggacgagaaa ggaatcgtcg
      181 aacagggcgt ttcgggccgg gcgtccggct tcccggcgcc tccggcggag accctcggcg
      241 acccggccgg gaaacgattc ccaatacccg gggaccacgg gcgcctcggc ggattcccgg
      301 gcgaaggcat ccaggcggcg gatgacttcc aactcggtcc ggcaggcggg gcaggcggcc
      361 aggtgttttt ccaggcggcg gcgcttgatt ccgcggagct cgccgtcggc gaaggcgccg
      421 agacggtttt gaagcgtccg gtgtttcatg accgcctcct ttcgacccgg tcggcaagca
      481 taaagcggag tttggcccgg gcccggctga gccgggacat gacggtccct ttggagattt
      541 tcaaatcgcg ggcgatgtcg cggtagctcc ggccctcgaa gacgcggagg acgaaaacgg
      601 cccgctgatc gaaaggaaga gcccccaggg cttcctggaa ggccccctcg gcctcgcggc
      661 gttggagctc atcctgaggc gtatcacggc ttggggccgg ttcccggtcg cccaggggct
      721 cttcgcggtg gaccgcccgg agatgattca gggaagcgtt gacggcgatg cggcggagcc
      781 aggcatagaa atcgcggcct tccttgaacc cggaaagggc gccgtaggct cggatgaacg
      841 tttcctgggc caggtcgtcg gccgcctgag gggcgccggc cattctcaag cagaggaagt
      901 aaatgggacg ctcgaaagtc ctgaccaggt tctcgtacgc ttccatgtca ccctctctcg
      961 cccggccgac cagcctccgt tcttccgtcg gatcgatgcg cctcatatga gctcccgttc
     1021 tgaatacacc ccaatttcgg gttttattcc ggccgatgac atcatacggc tcgatcggac
     1081 gtttggcaaa aacacgaagc cggcgtcccg accgccggaa accctttccc gtcgcggccg
     1141 gatgtgctat aaaaggacca tcttgcggct cccgcacgga ggagccgagg agggatcaca
     1201 tgattcagac gctcggccag attcccttga acacgctccg cgcctacgga aaaccagacc
     1261 agctccttta taaggcggac gggaaatacg ttccgatctc cgcccaggag ctttatgacc
     1321 gcgtccggca gatttccctg gggttgaggt cgctgggctt gggggcgggc gaccggctcg
     1381 tcatcctttc ggagaaccgt ccggattggg tgatgacgga tttcgccgtc ctggccgctg
     1441 gagcggtgac ggtgccgatc tatccgaccc tgacgcccga gcagatccgt tatatcgtcg
     1501 atgacagcga ggcgaagatc gtcgtctgtt cgacgcccga cctgcaggcc aaggtggacg
     1561 cgatccggcc gtccttgtcc ctcgtcgctc atttcctctc ttttgaggat cagcccgccg
     1621 ggggattcat ccccttcaag gacgtggttg aaaagggccg gcaggccgag gcggccgatc
     1681 cggaggcctt cgagcgggcc gccgccgcgg tcaagccgga cgacatcgcc tcgatcatct
     1741 acacgagcgg gacgacgggc gtccccaagg gggttatgct gagccatgac aattttttca
     1801 gcaacacccg ggcgctcgac gccgtgaccg atttccactg cggcgacacg atcctttcct
     1861 tcctcccctt gtcccacgtc ttggaacgga tgacgatgtt ctcgttcctc tacaagggct
     1921 cgaccgtggc tttcgccgag agcatcgata ccgtcgccca gaatctcctg gaagtcaggc
     1981 cgacgatcat ggtcagtgtt ccgcggctct ttgaaaaact gtatgcccgg atcatggaca
     2041 gcatcctggc cggatcggct ctcaaaaaga aaatcttttt ctgggccctg cgcggcggac
     2101 tgagcgcgag ccggaaagcc cagcggcgga cggccgccct tttcccccgc cggagcctgc
     2161 gcctccgcct cgcccagaag ctcgtttttt cgaaaatcct ggagaaaacc ggcggccggg
     2221 tgaagttctt cgtttccggc ggagcgccct tgtctcggga catcgccgag ttctttcacg
     2281 ccatcgggct ggtcattctc gaaggctacg gcctgacgga aacctcgccg gtcgtggcct
     2341 gcaacacctt cacgaaaatc cgtttcggaa cggtcggccc ggtcgttccg aaagtcgagg
     2401 tccagatcgc tccggacggg gagatcctcg tccgcggccc gaatgtcatg aagggctatt
     2461 tcaagaaaga agcggaaacg gccgaggccc tggccggcgg ttggctgcac acaggggaca
     2521 tcggccatct cgacgtcgac ggcttcctgg tcatcaccga ccggaagaag gacctgatcg
     2581 tcacggccgg cggcaagaac gtcgcccccc agccgattga aaacctgctc aaacaaagct
     2641 cctacatcgc ccagaccgtc gtcgtcgggg ccggccgcaa gttcatctcg gtcctggtcg
     2701 ttccgaattt cgagaaactg gaggcctggg cccgcgagcg gaacatcgcc ttcgccgcga
     2761 gaaaggattt gtgcggccgc gacgatgtcc gggatttcta ccgggaggaa atcgaacgct
     2821 gcacgccggg cctcgctccc tacgaaaaaa tcaagaaaat ccttctcctc gaaagggaat
     2881 tcgagatcga gcgggacgag ctgactccga cgctcaaggt caagcgctcc cgggtggagg
     2941 agaagtataa agccttgatc gacggcttgt accgggagtg agggggagga ggaagtccgt
     3001 gaaaatcttc cggggaatgc tgccggcggt cgtggcggcc gccaccgtct cgtgtgtcat
     3061 cccggtctat gtggacgagg ggagccgctg gagccgcgag cctgaagcct tccagcaggt
     3121 ttcggagttc gcggccggag gcgttcttcg agtcacgaac atctatggaa acatcatcat
     3181 ccgcggctgg gaccgggagg aactggaggt cacggccgag gaaacccgga acgattccgc
     3241 ggcgtccatc ccccgggtcg agatcgcttc cgaaggcggc gaaacggcga tcacggccgg
     3301 tcccggcgac gaggatttcg ccggcggccg gaccgtccat ctcctgatcc gcgtcccgca
     3361 ccgggtcttc ctccggagtg tcacggccgg ccggggccgc ctccagcttt ccgaccttta
     3421 cggcgaggct ttcctgcgcc ttgacgacgg cgatctccgc gttgaaaact actccggcag
     3481 cctggacgcc gagatcggcc ggggttcgat ggaggtcgag cttctcgacg cccgcccgga
     3541 ggacgccgta cgccttgtcc tggacgaagg cccgatcgac gttctcttgg agcccggttt
     3601 cgaaggccgc ttggaagtcg aggccccgga ggggacggtc tcgtgcgatt ttgaagtcac
     3661 tccggcggcc gaaccggccc gcgtttccgg aacggtcggg tcgggcgagg gcgccttggt
     3721 catcgtttcg acccggcatg ggagcgtgga cgtccgcaaa acgaaataaa ggaggggccg
     3781 tggaggagaa gcagaaaatc atccgggcgg cggtcgtcgt tttaatcgtc gtcctcctgg
     3841 gcctctgcgc ctattactac tttttttccg gacggctgaa aaacgccgac ggcggaatca
     3901 ccccggccca ggccgaaaaa atcgacgcgg aaaagccgcg ggagatcgac gcggcggcgg
     3961 ccgccccgct ggccgttccc ctggacggaa gcgatgaggc cgtccgggct ttggccgggg
     4021 agatatcctc cgatcccgtc ctggcccggt ggctgcggag ccgggacctg atccggcggt
     4081 gcgccgcggc cgtcgatgcc gtggccaacg gccaaagccc ccggactcaa ttcgatttct
     4141 tcatccccgg gggaccgttc aagacgtacg tccgcggggg cgaaacctat ctcgatccct
     4201 cctcctatgc ccgctacaac accgcggccg acgtcttcga cagcctcggg accgcggcct
     4261 gcgcccgggc gtacgcccaa tgtcgcgacc tcctgcagca agcttaccgg gaattgggat
     4321 acccggcggg cgatttccac cagacccttt accgggcgat catcgagatc ctgagaacgc
     4381 cggtcctgga acagccgatc gcggtgaaaa aagacatcac cacgtatctc ttcaaggacg
     4441 aaaagctcga atccctggac gacgtccaga agcatctcct ccggatggga ccggaaaacc
     4501 tccagctcat ccaagtgaag ctgcgggaga tcgccttggc cttgggattc accgaagccc
     4561 agctgcccgt tccgcggccg taccggtgat tttcgccgga tcaggcgttc cgccgcttcc
     4621 aggggaaggg atctttccgg tcgaagtcgg gattcaagtg ccggccgtcg gcgaaaagct
     4681 ccggctgggc gaacaaggga tcgaaaccca gccggcgggc gcggtcgaga acggcctcgt
     4741 attccccggg atgaagcggc cggtcgaggt cggggggaac gcgccagcag ggccggaact
     4801 ggctcatcag gcttaacggg acggtcgggc ggaaggtttg ggccagccag tcgaggacgg
     4861 ccagggaatt aggaacctgc ccgggcagaa cgagatgccg gacgatggtt cctcgaacgg
     4921 cccggccctc ctcatcgagc tccaggtcgg gctgctggac gaacatttcc tggagagccg
     4981 ggccggccca ttcgaaatag tcggccgccc ccgacagccg ggcggcgggt tcggacaggg
     5041 catacttgaa gtccggcagg tagatgtcga tgatgccggc cagccgttcc aggacatccg
     5101 cccgttcata tccgttcgtg ttgtagacga ggggaagaga caagccccgg atcaaggcga
     5161 gacgaagagc ccgcaggatg gggagaaggg actgagtggg ggagacaaga ttgatattga
     5221 gggcgccctg ccgctcgagg tcgagcatca agccggcgag ctcctcgtcc tcgaccgggc
     5281 gccctctccc caaccagctg atctggtagt tttgacaaaa gagacagcgg agattacagc
     5341 cgcagaaaaa gatcgtccct gagcccagcc ggcgccgacg gcctgtttcc ggaacgcagc
     5401 cgtcggaaag cccgctgagg acgggttcct ctccgaaatg aagcaaggac gaggcgacga
     5461 cggccttgac gccggttcgg cagaagcccg aaggcccggc ccggcggtcg gtccggcatc
     5521 ggcggggaca gaggtcgcag gcctcttccc ggcctttcca ggcggaaagg gccgcctcga
     5581 ttctttcgat tttcgtcatt tcaagcgtga ggagggctca gaaggctccg agctggcatt
     5641 tccggatttt cagcccggcc cggtcggcca gccagccggc gtgcagccgg gagaggccga
     5701 atctcgaggc caacgcccag atgtccgaac aggttatgct ccgatcgttc cccccggctt
     5761 cccggagggc gttttccaag ccgtccggca ccggccgggc ctcgagcccg gctcccgtcc
     5821 atcccttggc gtgaccggga tatccgaagg ttccgatctg gcagcgcccg agagtcacgg
     5881 ccatcgcgtc gagcgtccgg ccgacggcct cgggatcggt gtttttcgtt tcggccaggc
     5941 gcatggccgc ggcgcaagaa acggttccgt cggattcccg caggccgggg acggccgcgg
     6001 ccagctcagg gtcgggcttc aaattcgggt cgatcgcttt gtctttcagg gccatgaaca
     6061 tctccttgtc tgtcacccat ggtaaaggga tggaagcctc aagtcaacgc gccgtcccgg
     6121 cgtttgaagg cgcgggccgc ctgtgatacc atttcggcct aaccgattct ccgaacggtc
     6181 cggcctaagg agccccgtct cgacggccgg ccgtcgcagc gccgtcatcg acggacgaac
     6241 ggcggggatg gaaacgcccc cgccccccga tcctggaaag gaggatgccc gtgggtcgca
     6301 taaaattcgt ctttatcgtc attctcatcc tcttcgcggg gaccgccttc ggcgccgcgc
     6361 cggacggccc cttggagttt caggacgcgg ccggccggac gatccggctg gaaaagcccc
     6421 cggaaagaat cctggcgatc ggacacgggc cccagatcat cgcccatttg atgttcatgt
     6481 tcccggaaag ccggagccgg ttcatcggct gggaacggag ggggagaatc gccagcgaat
     6541 tcatcccctt gatcgatccg gatttcgaca agaggtattt caccgatccg aacacgggcg
     6601 tggaggaagt ggccgcctct catcccgacc tggtcctgat ccggggattt gtaaccgatc
     6661 ccaaggggga cgccctggaa aaggtcggca tccccgtggc ctatctcggc ctggagaatc
     6721 cggaacaata ccggaaagat atcgagctgg ccggcttgat cctggggaat cccggccggg
     6781 cggccgagat caaccgtttt ttccaagaac ggctcgaccg gattgaacat gccttggccg
     6841 gcctggagga ggcccggaag ccgtccgtct ttttagccat gaccatgtcc cgcggcggca
     6901 agatcgccgt tcgggtgccg gccccctcgt ggatccagac ggtcatggtc cggctggccg
     6961 gcggaaggcc ggtctgggcg gagggcatgg tggacacgtc cgcctggtcg gtcgtcaata
     7021 tcgagcagat cgcccgctgg gatcccgacg ggatcatcct cgtcgtctgg cataccctcg
     7081 acccgcagaa aacgctcgcc gccttgaaaa ccgacgagac gtggcgggcg ctgcgggccg
     7141 ttcagggcga caagatgcgg gcttttcccg ccgatatcta cggatgggac acgcccgatc
     7201 cccgctggat cctcggcttg acctggctgg cccggacgtt tcatcccgac cgctttcccg
     7261 gactcgacat gaaagccgag atcgtgtcct tttatcggga tctttacggc ctgagcccgg
     7321 acgacgtcga ggcgaagatt ctgccgtcga tccggaccgg ctactgagcg atggactccg
     7381 ccgccgaccg ccgcgtccgg atccgccgga tctggctatt ggcgggccta tcggcggcgg
     7441 ccttggcggt ctccgtcctg ctcgggcgat accccggtcc ggtcttgacg ccgccctcgg
     7501 tgatcggaac cgatacgctc gcccgggatc tcgttttcca tatccgaatt ccccgcgtcg
     7561 ccgcggcctt cgtcctcgga gccgctctct cggcggccgg ggcggtcttt cagaccgttt
     7621 tccgcaatcc cttggtcgac gccgggtttt taggcgtttc ccaaggggcg gcgttcggcg
     7681 cgtcggccgc gatcgtcttc gcctcgggga acgcctgggc ggtccagggc ggagcggctc
     7741 ttttcgcttt tctcgggctc gccttaagct atgccctggc gacgcggatc cgggtggggg
     7801 atgagatcct ccacctcgtc ctggccggga tcgtggtcgg agcgttttat gccgccggaa
     7861 caggaattct gaaatatatc gccgatcccc aaaaacagct ccccgacatc acttactgga
     7921 tgctgggcgg actttggggg atcacggggc gggacgtcct gcaaatgctt cccgtcgcgg
     7981 ccgccggcct ggcttacatg ctggccgttc ggtggcggct gaacctcctg gccatgaggg
     8041 acgaaacggc cttttcctta ggcgccgccc ccggccgcga gcgcttgttc gtcctgatcg
     8101 cggccgtggc cgtgaccgcc gccggggtgg ccaaggccgg gcagatcgcc tgggtcggcc
     8161 tgatcgttcc ccacatctgc cggcgcctcg tcggctcgga gacgagggtt ctcctcccgg
     8221 cctccgtcct cctgggcgga attttcgttc tcgtctgcga cgacgtggcc cgggtggccc
     8281 tgagcgggga aatccccttg ggcatcctga cgtccctcct gggcaccctg cttttcctcg
     8341 tcctgcttct ccaaagaaaa atccggggcg tcggatgatc gagctccgcg gcgtctggtt
     8401 ttcatacaac gggcggacgg agtccgttct gcgcgacgtg agcctgtctt tccaccccgg
     8461 aacggtgacc gccttggtcg gaccgaacgg ctccggcaag acgacgctgt tgaacctcct
     8521 cttggggtgg atcgcgccca aggaaggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnngcc ggaaagcccg tccggacgct cagcacgggg gagcgccaac tggcctcgat
     8701 cgcccgagcc ctggcccagg atcccggcct tctcctcctc gacgagccga cctctcacct
     8761 ggacctggcc aatacccggc gcgttctccg gatcctgcgt tttctccggg accggggcaa
     8821 gacggtcgtc ttgacgactc atgatcccaa cacggcctcc gtgacggccg accggatcgt
     8881 cctcctccac gaaggccggg tggccgcggc cgggacgccg gcggaggtca tcaccggagc
     8941 gcgcctgagc ggcgtttacg gcgtcgagat cgacgttcgg atgatcgacg gctctccgca
     9001 cgttctggct aagctttgaa accggcggaa ttccgccgct tggattccgt ttgacaggcc
     9061 gcggtcgttc ggatatatct ttttccggct cttttccatt tggcgaaagc ggatcgcgga
     9121 agcggcggcg gccggagcga aaacgttcaa gcgttcggcg cctggaaccg gtgaaaagcc
     9181 tcccgggacg cgggctcccc gccgggccaa gccgggcggg aaattccgcc gcgatccgga
     9241 cgagagagcg gaaaccgtcc tgtccccgcc gcgttacggc ccccaaatgg gaagaagcct
     9301 gattttttct tcgtcgaagg agtcttcatg gaattgatct cgcaggccgt cgatttcgtc
     9361 ctccatctgg acaagcacct gaacggtttg atccagatgt tcggcggctg gacatacggt
     9421 ctcctttttg tggtgatttt ctgtgaaacc ggcctggtcg tcaccccctt ccttccgggg
     9481 gattccctca tcttcgccgc cggagcgttc gccgcggccg gatccctcaa gatcggctgg
     9541 ctgtttctca tcctggccgc ggccgcggtc ctgggcgaca cggccaatta ctggatcgga
     9601 aagatcatcg gccccaaggt cttcaccaag gagaagtcgc ggattttccg caaggaatat
     9661 ctcgaccgga cgcaccgctt ttacgaaaaa tacggggccg agacgatcat cctcgcccgg
     9721 ttcgtgccga tcgtccggac gttcgctccg ttcgtggccg gaatcggccg gatgaactat
     9781 ggcaagtttc tcagctacaa cgtcgtcggg ggagtggcct gggtgggctt gttcagcttc
     9841 ggcggttatt ttttcggcaa cctctccttc gtcaagaaac acttcagctt ggtgatcatc
     9901 gccatcatcc tgatctcgct cgtcccggtc ctttgggaat ttctcaggca ccggcgggac
     9961 aagaagaaaa cggaagggac cgcgggctga ggcagcgggt caagcccatt ccattccgcg
    10021 gaagatcttc cgcagcggcg aggcgccgga atccggaatc cagctcaaat cggtccgcag
    10081 cggggtgatc gaaacgtatc cccgctcgat cgtccggatg tccgtttcgg acgagccgtc
    10141 gaacaagacc ttgccggtcc cgatccagaa atagggctga ccgcgcgggt ctttcttttc
    10201 gaggacgtcc cgttcataga atttccggcc gaggcgggtg atccggaggc cgcggagcgg
    10261 cggggcgggg atgttgacat tcaaggtcac gccgtccggc aggcccgagg cgaggatccg
    10321 ctcggcgagg cggaaaacga cgtctgccgc gtcttcccgg taaaaacggc cagccgcgtc
    10381 cgggatcata gagacggcca gggaaggaat gccgaaaaat gtcccctgga aagcggccgc
    10441 gaccgtcccc gaataatggg cgtccagctg gccgaggttg ggacccgggt tcaggcccga
    10501 gatcagcagg tcgggccggc gggggaggac cttgtcgacg gcgagataga cgcagtcggc
    10561 cggcgtcccg tcgacgctcc agacgcccgg tccgtcgcgg tggagacgga ggggatggcg
    10621 gagggtcaag gccagggaaa aggcgcttcg ctcccggtcc ggggcgacga tcaccgcttg
    10681 gcccagggcg ccgaggcggc ggcggagaac gcggatctcc gcccggtggg ccccgtcgtc
    10741 gttgcaaaga agaatgagcg gctcggccgc gcacgtcata atcgatcctt tcatccggcc
    10801 gggcggcgga agggggaatg aagcatggtc gggacgggcg gattcgaacc gccgacccca
    10861 tgcaccccaa gcatgtgcgc taccaggctg cgccacgtcc cgacggaaga ggcattatag
    10921 caaaaacgtc cggccgggtc acccccgcgc attgaatcaa cgaaaaatct cgccgaaacc
    10981 ccaagaagga ccaaaagaca ggcacgggag ggccgggcga tcagggaaag aatgtctcaa
    11041 agctctttga gggcgatgtc tgaaggaacg aaaacgcgga aaaccagcaa gcttgagcgg
    11101 atttttcctg aagaccggtc ttcagagaaa gtcaatccgc cgggcggcgt tcttttttcg
    11161 ccggccggcc cagggattgg gagagatcct gcaattcgtc gcggacgcgc cagagcagct
    11221 tgacgatctt gtccggatgg acggccggaa ccggccggag atccatttcc gcctcgagac
    11281 ggcgccgggc gccggccaag gtgagctgtt ccttcccgag aagatccttg atccggagga
    11341 tgatctcgag gtccttcgcc cggtagatct tccggcccga agccgtctct cccggttcga
    11401 tgaagggaaa ctctttttcc cagagggcga tcgtttcggc atcgattttc gccagccggc
    11461 agacttccgc gagccggtag aaaagctttt gttcgtcctt cgggctcatc ggatcttcgt
    11521 cactccgcgg tcggcgctcc ccccgcgtgg ttgattcccg gttacgatag catgggccgg
    11581 ggccgcggtc aatccaaccg gccctcattc cgggcgaaat ggggtataat ccccgcatgc
    11641 ccaaggcgaa gccggagacc ctcgtgcccc tggccgagcg gatgcgtccg gaaacgttcg
    11701 atcggctgta cggtcaggag catctcctgg gcaagggcaa gatcctgacc cagctcatcg
    11761 aatcccgccg tctcgtctcc ctcatctttt gggggcctcc cggctcggga aaaacgacct
    11821 tggccatgat gctggcccgg cacttcgacc tcccctcgac gtttttcagc gccgttcttt
    11881 ccgggatcaa ggaagtcaag gaggtgatgg cccaggccga aagccacaag cgcctctacg
    11941 ggaagccgac gatcatcttc atcgacgaaa tccatcgctt caacaaggcc cagcaagggg
    12001 cttttttgcc ctatgtcgag cgcggcgaca tcatcctctt cggctcaacg accgagaatc
    12061 cctcgttcga ggtcatcgcc ccgctccttt cgcggacgcg ggtgctcacg ttggagccgc
    12121 tcgacgacga ggcggtgagc cggatcctcg acgacgccct ggccgatccg gaccggggga
    12181 tcggccgacg gggcctgagc ctcgatccgg cggcccgccg gatgctcatc gaccaatcca
    12241 acggagacgc ccggcggagc ctcaacacct tggagctggc cgccgacctg gccggctcgg
    12301 gcgtcatcgg cccggatcac gtccgggaag cgcagcagaa gcgcatgctg ctgtacgaca
    12361 aggacgggga ggagcattac aacctgatct ccgccctgca caagagcctg cgaaacagcg
    12421 acgtcgacgc ggctctttac tggctcgccc ggatgctcta tgccggggag gatcccttgt
    12481 acatcgcccg ccggatggtg cgtttcgcct ccgaggacgt cggcctggcc gatccccagg
    12541 ccctggcggt gacgcttcgg gccaaggaag cctacgagtt cctgggatcg ccggaagggg
    12601 acctggccct ggccgaggcg gtcgtctatc tggccgccgc gccgaagagc aatcgcgtct
    12661 acgaggcgtt cgggaaggtc cgcaaggatg tcgaggagcg gccccatgag cccgttccga
    12721 aagtcatccg gaacgccgtc accgccttga tgaaagaagc cggctacggg aaggggtatg
    12781 cctacgccca cgaccggccg gaaggaacga cggacatgga gacgatgccc gaccggctgg
    12841 ccgggaggcg ctactacgat cccgggccgt tcggctatga aaaggaaatc cggaagcgga
    12901 tggattggtg ggccggagtg aaggcccgtt tccgctcgga aaaagaaaac gccgggacct
    12961 gatcaggttt cggatttttc cttcatcagg taataaatct cccagctgag gtcggtggcc
    13021 aggcaatcga ggcaggagct cggggtcgtg gcgatttccc ccaaagccag cttgtccttg
    13081 atcatcgcct tgaccttttt cttgattttg taaagcaaaa tatagttgac catggcattc
    13141 ctatcaaata aatagcattt atcgccccgg ttgtaaagcc cggcgcccgg ctcatgatat
    13201 aatatcattc cttgtcatga aaacgacgcg ccttttcgcg gtcccggcga ttctcgctct
    13261 gctttcggcg tcccgcggaa tcccgcggct gaatcccgag gagacggccg tcctgaagtt
    13321 tcccttggtc gaggcctcgc gagtgacctg ccgagggccc ctggttccgg cgatcatccc
    13381 cggtccgggc ggccttcttc tcgtgcagtc gcggacggga ttccttcagg cggtcgaccc
    13441 ggaaaaatcc gtgattctct ggaccgttcc cgtgggatcg ggagccctgc cgcccgtcgt
    13501 ctccggcgac cggatcgtcg ccgcctggag caacggccgg gtg
//
LOCUS       DAOOXH010000066        13512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_4644_length_13512_cov_8.564613, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000066 DAOOXH010000000
VERSION     DAOOXH010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 13512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13512
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4644_length_13512_cov_8.564613"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PKV64_08235"
     rRNA            complement(<1..333)
                     /locus_tag="PKV64_08235"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            928..1377
                     /locus_tag="PKV64_08240"
     CDS             928..1377
                     /locus_tag="PKV64_08240"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPN17103.1"
                     /translation="MNKPIFLADADGLRRLEIPAVRTEEKGFSLIEVLFAAGLISFLL
                     AGTAELLLTSIEVDRKAGRKVNLTGLLSSELDEFKAKPFDSPDLAPGGGEIVLRPETD
                     GPGVIVAWTVEAVSANLKKVSFTLTREGRTDQPLEAVLLITPELAGR"
     gene            1374..2102
                     /locus_tag="PKV64_08245"
     CDS             1374..2102
                     /locus_tag="PKV64_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17104.1"
                     /translation="MRKRAEDRSPGFSLLEALLSLTIFFLIFAGSLEFFGTARRVFFR
                     LLSRQENHQAAWAALDRIRSDVLLAGRGLVRPMRLGIVAPAEEAGGRWVLVRAGAAPV
                     LTAGPRAGETTVAVAGANPDWAGKTLCLYDRTRGEKAVILEAVGGILTLSSPLDCDYR
                     IPEASAAVLQTTSLFLDAGQGVLRRKSDSSSAQPLLEDVHSFTFIYDSETCLAEARLS
                     LASAPEEFHALKILARNALLGKMR"
     gene            2053..3648
                     /locus_tag="PKV64_08250"
     CDS             2053..3648
                     /locus_tag="PKV64_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17105.1"
                     /translation="MPSKSWPETPCSEKCADGRITGRTGSITMGVVFASFLISTLGLG
                     LCLLTWTYRQWSAYKSDAVLIREAAENGARAGQAALDEILAGRPFPLILAEGEYEALR
                     AATLAGETDAVAAALGTGLPLTAAETAGGWEWSSETEFRAVRVADSESFFGAAFAATI
                     EARGRLSRRPQNKRTALDLGLSLMAGRVPLSSFPFLLAGENGPENAAALLAEGRVVLA
                     PPESGSASPLPAAAGLPLIPADPEPLVAEALRIKIFSPDKLTVLQLRRALGLPLVDEP
                     VPDGVYLAVSEAVPGGIFIQGDVDEMILAAGDGRQHLQVRLEEGLWRMIFSPAEDVLE
                     MTGPDGIPSLGRAPLPVVLINGSVASLGGGLIETDGTLSLTDSPDAPSLLAGLSLTLV
                     SAGEIVISTHLIQDGVRWKDGIPYLKDASTQLFIYASGRDLVTGARADGRIRVAAEAP
                     AGLHLQASLTARDGVRLEGTRHEVTVSGGLQASCLETAGALLTIRPDDRLTSDLADGA
                     SSPRAAVPVLFISGREILQWTDR"
     gene            3633..4076
                     /locus_tag="PKV64_08255"
     CDS             3633..4076
                     /locus_tag="PKV64_08255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPN17106.1"
                     /translation="MDRPMTGRGFTLIEAVLALAILAVIITAASLSFLNLAAKYRLEK
                     AVGEVRTALVSARAKSILNGRRFRVRFGADFYRIETYDENSGLWLPDASAGIEGAAVT
                     ANNAPVFTPEGGVTNMATIKIFNGWGAYKMTLAITGRIKIARLPS"
     gene            4321..6723
                     /locus_tag="PKV64_08260"
     CDS             4321..6723
                     /locus_tag="PKV64_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPN17107.1"
                     /translation="MTKPLPLFISILTLAAAASVPFLAAQEPYKTPPKEIVDILSAPP
                     TPRVLLSPGGDRLLLIESESMPSIATLARPFLRLAGMRLTPADNSRQMTVFDTALTLK
                     TIKDGKDQGIELPAGIKLARPAFSSDGRWISFARFLENGVELWVAETATGKARALTPP
                     RLNLVLSGIAWMPGNRKILVQMIPGDRGPAPVEPRVPVGPTIQESGGRQVKVATYQDL
                     LKNPFDEELFEYYATGQISLVDAVSGRIEPLGAPGVFLSITPSPDGRYLFVDRLKRPF
                     STSVPYYDFAHSAEIWDLSGNAVKVFPDLPSGENTPMNGVPTGPREFSWQARKPATVL
                     WVEALDGGDPEQAAEFRDKIMALDAPFSGEPREVLKTGKRYEGLAYFETPGRALAYEV
                     ESKAYRRVIRLVDLDHPETAPRVLFDLDVQDAYANPGDPVMTWKDGERVILQDKNGIY
                     FSGRGASPRGDHPFLDLRDIKTGAKRRLFQCADGKYQAFIGFSGQERRDIVITQESRT
                     EVPNYHLYSLKTGKTRPLTAFADPAPRMTGVKKQLIKYNRSDGIELSGTLYLPADYQE
                     GTRLPVVIWAYPLEYGSSATAGQVRGSTDRFTFYRGASQLFFVSQGYAVLDNATMPVI
                     GDSKTVNDTFVDQIVGNAKAAIDALDRLGVGDPRRVGVGGHSYGAFMTANLLAHSDLF
                     AAGIARSGAYNRTLTPFGFQSERRSYWEAPETYLKMSPFMCADKIKTPLLLIHGMNDN
                     NSGTFFIQSERLFAALKGFGATARFVSLPGESHGYLARESVFTVLAEMIEWFDAYVKN
                     RK"
     gene            6766..9402
                     /locus_tag="PKV64_08265"
     CDS             6766..9402
                     /locus_tag="PKV64_08265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPN17108.1"
                     /translation="MTIRSKSFLRLPPLLLIAGLFLLALPPRSLQAATAAEEPTSEKP
                     VPAWLRLGPFACPPPAFADDPAFGFGPAELLEFEELEIGGVRPAAGRGLRWSDGTSAA
                     WTEVPCGEAGLPLEAAGPGPKTAYLAAYVEAFRFQKARLTVRTGQLHRVFFDGRQVAS
                     QVKADVDGRTTADLKLETGVHLILIKTVYDPNSPTGWNVRASLEFAGSPAGPEPQMTV
                     SPGEKVALRHILDGPRAAGVSLSPDGKNLALAMTRIMSPAGEAESWIELYRIETGRDG
                     LSARLTRSLRGGTAVSGIVWAPRGNRFSYTTQDKNGGSIWLVDADSGETRPLLRNFKN
                     LGFHAWAPDGNALVFSGTEEGPKDSALAKRYRDPEDRQPGQRDRRHLYRLTLPDGVRE
                     RLTAGELSADFGGFSPDGATLLFTRNPVDMTERPYSRTELYLLDPATRKEDLLWKGAW
                     FNGARFSPDGKTLLVLGGPSAFGGIGLNVPPDRLPNEYDIQAYLFDLADRTARPITRE
                     FNPSIERAFWSGDGKVIRFLTTDGARTRLYDYAPAGKTFVPRESGVDVIERIDIASAA
                     PRAVFVGSSPQTPPRVFLWDLKTGKIRPLAGPLPGSEIAAGKVETWTFKNRRGVEIDG
                     FVVYPPDFDPARKYPLIVNYYGGTTPITRDFGGRYPKALYAAHGYIVYVPEPSGAIGY
                     GQEQSAFHVNDWGEITSEEIIDGLTAFLAAHPFVDPKRVGCIGASYGGFMTMTLATKT
                     PMFAAAISHAGISSISSYWGEGYWGYTYSAVATAGSFPWNRKDIYVDRSPLFHADKIT
                     TPLLLLHGSADTNVPPGESIQLFTALKLLGRETELISFADENHTIITYDKRILFNKTI
                     LAWFDRWLKDQPEWWFDLYPRK"
     gene            complement(9645..10757)
                     /locus_tag="PKV64_08270"
     CDS             complement(9645..10757)
                     /locus_tag="PKV64_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPN17109.1"
                     /translation="MKKTLIPALLLILLPASLAFAANDASAENTGIAPGSYKSWSALP
                     ILMYDNDIGFGYGAKAKLVDFLGIKESFDLILFNSSKGERWYVFTFSLPDIEIRQGKT
                     YPLSFDLKAEYDKFLKYSYYGQGMDSRKEDETILTHEVKNLFLTVGRGFTPHFSASVA
                     YVFRTIDYYGVQDGPLAGELRRLKKKSAPYFSLAMAYDTSDSQIHPTRGLRLLIQDDI
                     AVSFSRNGDASFNRVTFDFRKYQRVFGEKDVFAFRTLVQYINGDEIPLFDLSSLGGGN
                     TFNVMRGFGLNRFLDKGKILANVEYRFPVWWIFGGNVFIDAGNVWPSFGDINFRKTVF
                     DYGVGLRLFMKDFCARVDFGFSKEGMRLYFNFGHVF"
     gene            complement(10809..11498)
                     /locus_tag="PKV64_08275"
     CDS             complement(10809..11498)
                     /locus_tag="PKV64_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase domain-containing
                     protein"
                     /protein_id="HPN17110.1"
                     /translation="MFFSISPRENADRLRETARDLGFSVAGVADVSSVRETFRLPENL
                     RNRFVRAAVLGVRLLDGVLEDLVDRPTPLYFHHYRQLNFFLDREAFRLAARIQAAGFL
                     ALPIPASQILDSQRQTAHVSHKKLAVLAGLGHIGRNNLLVHPAFGSRLRLVTVLTDMP
                     LEAGIQTKADCGTCAACLASCPAEAIKMNPAEFDHRACFEKLDEFRRIRLVSQHICGL
                     CVKACRGRGQA"
     gene            complement(11764..12963)
                     /locus_tag="PKV64_08280"
     CDS             complement(11764..12963)
                     /locus_tag="PKV64_08280"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HPN17111.1"
                     /translation="MEVTELARAGISGVLAGFLSLGFSSCPGGKPAERLFSALGSAPA
                     GFNRPGSGNRAVLEYRLGEKESRVRISVEDRTWTEVLPAAGRIERSVLGFIKETARPS
                     ASRRSLAAAAGRIAGELIPFDRTLTALGVDCLIVIPDGILCRLPFEALRTADGRGFLV
                     ERYAISYAPFRFGPSLDRSEEEPGGSCLLAMGASVPDRPAGAGEADAVFEEMPGVREE
                     IRSVSRFFDRNRRFVYLDGAASEATLRRHRGGRYRLVHIAGHGTTEAGDPGRSSLLLC
                     RSAGDDGLLRAEEARALGLAADLVVLSGCRTACGDAPPKEAGGLPRAFLDGGARSVVA
                     TLWAVPDRSTADFMKEFYRHLEAGRGKSRALQAAKLGMIHGGRPHPFYWAGYILLGEP
                     EETILFN"
     gene            complement(13053..>13512)
                     /locus_tag="PKV64_08285"
     CDS             complement(13053..>13512)
                     /locus_tag="PKV64_08285"
                     /inference="COORDINATES: protein motif:HMM:NF023270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HPN17112.1"
                     /translation="RELVGHHVSGLLKIAPEHVSKNVLRLMNKDSGGAFEKFRRAFRK
                     ADVSGRRHLKYYFMVAHPGCGPREAGELAREVRRLEKEGDRPVEGVQIFTPAPMTLST
                     AMYHTGRDPRTGESVYVPWTFQEIKDQKRMLMERRPAGASVRPARRTRNR"
BASE COUNT         2586 a         4423 c         4007 g         2496 t
ORIGIN      
        1 tctgggccgt gtctcagtcc cagtgtggcc gttcaccctc tcaggccggc tacccatctg
       61 agccttggta ggccgttacc ccaccaacta gctaatagga cgcaggctca tcttcaagcg
      121 ccttgcggct ttgattacgg gacttgtgtc ccggaatgtc atgcggtatt agactccctt
      181 tcgagagctt attccccact tgaaggaaga ttacccacgt gatactcacc cgttcgccac
      241 tcatcctaaa gttgccctta ggacgcgttc gacttgcatg tgttaggcac gccgccaacg
      301 ttaactctga gccaggatca aactctccag ttataagcac tcttgcgagc acttagcgag
      361 tttgatgatg gcaaattggc tcgatcacaa tcgctccgct ttttaattca aagatccgtt
      421 tctctctgcc cgagagagaa aagccccggc ccgaaacatt ccgatacttg tttcagcctt
      481 tcggctgtca gataggaaag ccaggtcaaa ggctcggata atatagggct ttccgaagag
      541 tttgtcaagc aaaaaaaata tttttccccg cacttttttt aattgacgaa aatctaaata
      601 gttacagaca ttttttcgtt aacggagaag cgttgttttt tgcatttttt cgttcgagcg
      661 aacagcgcgg aatttgtttt tgcacggttc ttgcatagac ggccgatttg cttgatccga
      721 tcaactccga catctgtttt ttcccgccgg cacgggggaa aggggagcct ccgggccgcg
      781 gtcgtcaaac cgcgttgacg gcccggcgga tccttccacg gcaaaccgga aatcacggtt
      841 gacggcggcc ggcccgcgct cctcttcccc cgcctggatc gattgttccg gccggttggc
      901 acgccgcccg cattctccgc cgtcgccatg aacaaaccca tctttctcgc cgacgccgac
      961 ggcctccgcc gcctcgagat tccggccgtc cgaacggagg aaaaaggatt cagcctgatc
     1021 gaagtcttgt tcgccgccgg gctcatttcc tttctcctcg ccggaaccgc cgagcttctc
     1081 ctgacctcca tcgaggtcga ccggaaagcc ggacggaagg tcaacctcac ggggctcctt
     1141 tcctccgagc tcgacgaatt caaggccaaa ccgttcgact cccccgatct tgcccccggc
     1201 gggggcgaga tcgtcctccg gccggagacg gacgggccgg gcgtcatcgt cgcctggacg
     1261 gtcgaggccg tctccgcgaa cctgaaaaaa gtgagcttca ccctgacccg ggaaggacgg
     1321 acggaccagc cgctggaagc cgtcctgctg atcacgccgg agttggccgg acgatgagaa
     1381 agagggcgga ggaccgcagc ccgggttttt ccctgttgga agccctcctc agcctgacca
     1441 tcttcttctt gatcttcgcc gggagcctgg aattcttcgg gaccgcccgc cgcgtgtttt
     1501 tccggcttct gtcccggcag gaaaaccatc aggccgcatg ggccgccctg gaccggatcc
     1561 ggtcggacgt cctgctcgcc ggccgggggc tcgtccgccc gatgcgcttg gggatcgtgg
     1621 ccccggccga agaggccggc ggccgatggg tgcttgtccg ggcgggggcg gccccggttc
     1681 tcacggccgg accgcgggcc ggcgaaacaa ccgtcgccgt ggccggagcc aacccggact
     1741 gggcgggaaa aacactctgc ctctacgacc ggaccagggg ggaaaaagcg gttatcctgg
     1801 aggccgtcgg cggaatcctg acgctgtcct ctcccttgga ttgcgattac cggatcccgg
     1861 aagcctccgc ggccgtcctc cagacgacgt ccctcttctt ggacgcgggg caaggagtcc
     1921 tccgccgtaa atccgattcc tcgtccgccc agccgcttct ggaggacgtc cattcgttca
     1981 cgtttatcta tgactctgaa acctgcctgg ccgaggcgcg cctttctctc gcctccgccc
     2041 cggaggaatt ccatgccctc aaaatcctgg cccgaaacgc cctgctcgga aaaatgcgct
     2101 gacggccgga tcaccggacg gaccggctcg atcacgatgg gcgtggtctt cgcgtccttc
     2161 ctgatttcca ctctcggcct cggcttatgc ctgttgacct ggacgtaccg ccaatggagc
     2221 gcgtataaaa gcgacgccgt tctgattcgg gaagcggccg aaaacggcgc ccgggccgga
     2281 caggccgcct tggacgagat cctcgccggc cggccgtttc ccctcatcct ggctgaaggg
     2341 gaatatgaag ccctccgggc cgccaccttg gccggggaaa cggacgccgt ggccgccgcc
     2401 ctgggaacgg gcttgccgct gaccgccgcg gaaacggccg gaggatggga gtggtcgtcg
     2461 gaaacggagt tccgggcggt tcgggtggcc gattccgaat cctttttcgg agccgccttc
     2521 gccgcgacga tcgaggcccg ggggcggctg agccgccggc cgcaaaataa aaggaccgcc
     2581 ttggatctcg gcttatccct catggccggc cgcgttcctt tatcttcttt cccctttctg
     2641 ctggccggcg aaaacggacc ggaaaacgcg gccgcgcttc tggccgaagg gcgcgtcgtt
     2701 ctcgcccccc cggaatcggg aagcgcctcc ccgctcccgg cggcggccgg ccttcccttg
     2761 attccggccg atccggaacc tctcgtggcc gaggcgttga gaatcaagat cttctccccc
     2821 gacaagctga ccgtcctcca gctccgacgg gcgctgggcc tgccgctcgt cgatgaaccg
     2881 gtccccgacg gcgtgtatct cgcggtcagc gaggccgtcc ccggcggcat cttcatccaa
     2941 ggcgacgtgg acgaaatgat cctggccgcc ggcgacggcc gccagcatct ccaagtccgc
     3001 ctcgaggagg ggctatggcg gatgatcttc agcccggccg aggacgtcct ggaaatgacc
     3061 ggaccggacg gaattccaag cctcggccgg gccccgcttc cggtcgtcct gatcaacggg
     3121 agcgtcgcct cgctcggagg aggactcatc gagacggacg gaactctgtc tctgacggac
     3181 agccccgacg cgccctccct cttggccgga ttgtccctga cgctcgtttc cgcgggggaa
     3241 atcgtcatct ccactcattt aatccaagac ggcgtccggt ggaaggacgg gattccttat
     3301 ctcaaggacg cgagcaccca gttgttcatc tacgcgagcg gccgggatct cgtcaccggc
     3361 gcccgggcgg acggccggat ccgcgtcgcc gccgaagcgc cggccggctt gcatctccag
     3421 gcgtccctca ccgcccgcga cggcgtccgt ctcgagggaa cccgccacga ggtcaccgtc
     3481 tccggaggcc tccaagcctc ctgcctggaa acggccggcg ccctcctgac gatccggccg
     3541 gacgaccggt tgacgtcgga cctcgccgac ggagccagct ccccccgggc cgccgtcccg
     3601 gtccttttta tcagcggccg ggaaatcctc caatggaccg accgatgacc ggccgaggat
     3661 tcaccttgat cgaagcggtc ctcgccctgg cgatcctcgc ggtcatcatc accgcggcct
     3721 cgctgtcgtt tctcaacctc gcggcgaaat accgcctgga gaaagcggtc ggagaagtcc
     3781 ggacggccct cgtttcggcc cgggccaaat ccatcctgaa cggacgccga ttcagggtcc
     3841 ggttcggggc ggatttctac aggatcgaaa cctacgatga aaattccggc ctttggcttc
     3901 ccgacgcctc ggccggaatc gaaggagcgg ccgtgacggc caacaatgct cccgtgttca
     3961 ccccggaggg cggcgtgacg aacatggcga cgatcaagat attcaacggc tggggcgcgt
     4021 acaaaatgac cctggccatc accgggcgga taaaaatcgc gcgccttccc tcctgagcgc
     4081 caggacctct cgagccgtcc ccttcatccg cggaatccgt tcactccgcc gccagatcac
     4141 cccgggcgag cgtatcgaga ccgctttccc ccggccccgt cgccccaaga agctaaactc
     4201 atgaaaaaag cggaagtttt gatgccgcct tgacacaaat ccattctccc gattgaatat
     4261 ttcggggcat ctgcgatata aagaatggtt attaatgaaa tttatttatg gaggcttccc
     4321 ttgacaaagc cccttcctct tttcatttcc attttgaccc tggcggcggc cgcgtccgtt
     4381 ccgtttctcg ccgcccagga accctacaag acgccgccga aggaaatcgt cgacattttg
     4441 agcgcgccgc ccacgccgcg cgttctgctg agtcccggag gcgaccgtct gctgctgatc
     4501 gaaagcgaaa gcatgccgtc gatcgcaacc ctggcccgtc cctttctccg gctggccggg
     4561 atgcgcttga caccggctga caacagccgg cagatgacgg ttttcgacac ggccttgact
     4621 ctgaagacga tcaaggacgg gaaagaccaa ggcatcgagc tgccggcggg aatcaagctc
     4681 gcccgcccgg ctttttccag cgacggccgt tggatctcct tcgcccgctt cctggagaac
     4741 ggcgtcgagc tctgggtcgc cgagacggcc acaggcaagg ccagggcgct cacccccccg
     4801 cgtctcaacc tggtgctgag cgggatcgcc tggatgcctg gaaaccggaa aatcctcgtc
     4861 cagatgattc ccggcgaccg gggaccggcg cccgtcgagc cccgcgttcc cgtcggcccg
     4921 accatccagg aatccggcgg gcggcaagtg aaagtcgcca cctaccagga tctgctgaaa
     4981 aacccttttg atgaagagct tttcgaatac tacgcgacgg gccaaatctc gctcgttgac
     5041 gcggtttcgg ggcggatcga gcccctcggc gcgcccggcg tgtttctctc gatcacgccg
     5101 tcgcccgacg ggcgctatct tttcgtcgac cgcctcaaac gtcccttttc caccagcgtc
     5161 ccgtattacg acttcgccca ttcggccgaa atctgggatc tttcgggaaa cgccgtgaag
     5221 gtcttccccg atcttccctc aggagaaaac acgcccatga acggcgtccc gacggggccg
     5281 cgggagttct cctggcaggc tcgaaaaccg gcgacggttc tgtgggttga ggcgctcgac
     5341 ggcggggatc cagaacaagc ggcggagttc cgggataaaa tcatggccct ggacgcgccg
     5401 ttttccggag agccgcgcga ggtcttgaag accggaaaac gatacgaagg cttggcgtat
     5461 ttcgaaacgc cggggcgggc cctggcctat gaagtcgagt cgaaagcgta tcggcgggtg
     5521 atccgtctgg tcgatctcga tcatcccgag acggcccccc gcgtcctctt cgatctcgac
     5581 gtccaggacg cttatgccaa tcccggcgat ccggtcatga cctggaaaga cggcgaacgc
     5641 gtcattctcc aggacaagaa cgggatctat ttctcgggca ggggcgcatc cccccggggc
     5701 gaccatccgt tcctcgacct tcgggacatc aagacgggcg cgaaacggcg gctctttcaa
     5761 tgcgccgacg gcaaatacca ggcgttcatc gggttttcgg gacaggagcg ccgggatatc
     5821 gtcatcaccc aggaatcccg gaccgaggtt ccgaattatc atctgtattc cctgaaaacc
     5881 gggaaaacgc ggccgctgac agctttcgct gaccccgccc cccggatgac gggcgtgaag
     5941 aaacagctga tcaaatacaa ccggagcgac ggaatcgagc tctcgggaac cctctacctg
     6001 ccggcggatt accaagaagg aacgcggctg cccgtcgtca tctgggccta tccgctggaa
     6061 tacggcagct cggccacggc cggacaagtc cgcggctcga ccgaccgctt caccttctac
     6121 cgaggcgcgt cccagctgtt tttcgtcagc cagggctacg ccgtcctgga caacgccacc
     6181 atgcccgtca tcggcgattc caagacggtc aacgacacgt tcgtcgacca gatcgtcggc
     6241 aacgccaagg cggccatcga tgccttggat cgcctgggcg tcggcgatcc ccggagagtc
     6301 ggcgtcggcg gacacagcta cggcgctttc atgacggcca acctcctggc ccatagcgac
     6361 ctgttcgccg ccggaatcgc ccgaagcggc gcttataacc ggaccctgac gccgttcgga
     6421 ttccaaagcg aacggcggtc ctactgggaa gccccggaga cttatctcaa gatgtcgccg
     6481 ttcatgtgcg ccgacaagat caagacgccg ctgctgctga ttcacgggat gaacgacaac
     6541 aactccggga cgtttttcat ccaatccgag cgcctcttcg ccgccctgaa gggcttcgga
     6601 gcgacggccc gcttcgtctc cctgccgggc gaaagccacg gctacctggc ccgggaatcg
     6661 gtgttcaccg tcctggctga aatgatcgaa tggttcgatg cctacgttaa aaaccggaag
     6721 taagcgccgc cgcgtttccc tgatgaaccg aaaggataca cagacatgac gatccgatcg
     6781 aaatcgtttc ttcgcctgcc cccccttctt cttatcgccg ggctgttcct cctcgctctc
     6841 ccgccgcgct cgctgcaggc cgcaaccgcc gcggaggagc cgacgtctga aaagccggtt
     6901 cccgcctggc tgcggctcgg tcccttcgct tgccccccgc cggctttcgc cgatgatccg
     6961 gcgtttggat tcggcccggc cgagctcctg gaattcgagg agctcgagat cggcggcgtc
     7021 cggcccgccg cgggacgggg attgcgctgg tcggacggga cgtcggccgc gtggacggag
     7081 gtcccctgcg gcgaagccgg cctccccctc gaggccgcgg gccccggacc gaagaccgcc
     7141 tacctggcgg cctacgtcga agcgttccgt ttccaaaagg cccgtctcac ggtgaggacg
     7201 ggacagcttc atcgggtctt tttcgacggg cggcaggtcg cttcccaagt caaggccgac
     7261 gtcgacggcc ggacgacggc cgatctgaag ctcgagacgg gcgtccacct gattctcatc
     7321 aagaccgtct atgatccgaa ttccccaacc ggctggaacg tccgcgcctc cctggaattc
     7381 gccgggagtc cggccgggcc ggagccgcaa atgaccgtct cgccggggga gaaagtcgcc
     7441 ctccggcaca tactggacgg cccgcgggcg gccggcgtct cgctctctcc cgacggaaag
     7501 aacctggccc tggccatgac ccggatcatg tccccggccg gcgaggcgga atcctggatc
     7561 gagctttacc gcatcgagac gggacgggac ggcctctccg cccgcctgac ccggtcgctg
     7621 cggggcggaa cggcggtctc cggaatcgtc tgggcgcccc gggggaatcg cttcagctat
     7681 acgacgcagg acaagaacgg cggatcgatc tggctcgtcg acgccgattc gggcgaaacc
     7741 cggccgctgc tccggaattt caagaacctc ggctttcatg cttgggcgcc ggacggaaac
     7801 gccctcgtct tctccgggac ggaagagggg ccgaaggatt cggccttggc caagcgttac
     7861 cgcgatcccg aggaccggca gccgggtcag cgcgaccgcc ggcaccttta ccgcctcacc
     7921 ctgccggacg gcgtccggga aaggctgacg gccggagagc tgtcggctga cttcggggga
     7981 ttttctcccg acggcgcgac gcttctcttc acccgtaatc cggtggatat gaccgagcgc
     8041 ccgtattccc ggaccgagct ctaccttctc gacccggcta cccgcaagga agacctcctt
     8101 tggaaaggag cttggttcaa cggcgcccgt ttcagcccgg acggcaagac tcttttagtg
     8161 ctgggcggac cttcggcgtt cggcgggatc ggcctcaatg tccccccgga ccgccttccc
     8221 aatgagtacg acatccaagc ttatctcttc gatttggcgg accggacggc ccggccgatc
     8281 acccgggaat tcaatccgtc catcgaacgg gccttctggt cgggcgacgg caaggtcatc
     8341 cgcttcctga cgaccgacgg ggcgcggacg cggctctacg actacgcccc cgccgggaaa
     8401 acgttcgttc cgcgcgaatc cggcgtggac gtcatcgaga ggatcgacat cgcctccgcg
     8461 gccccccggg cggtcttcgt cggttcctcc ccccaaactc cgccccgtgt cttcctctgg
     8521 gatcttaaaa ccggaaaaat ccggccgctg gccggccctc tccccgggtc ggagatcgcc
     8581 gccggaaaag tcgagacctg gacattcaaa aacaggcggg gcgtcgagat cgacggtttc
     8641 gtcgtctatc cccccgactt cgatcccgcc cggaaatacc ccctgatcgt taattactac
     8701 gggggaacga ccccgatcac ccgggacttc ggaggccgct atccgaaggc gctctacgcc
     8761 gcccatggat atatcgttta cgtacccgag ccgagcgggg cgatcggcta cggccaggaa
     8821 caatccgcct tccacgtcaa cgactggggg gaaatcacgt ccgaggagat catcgacggc
     8881 ctcacggcct tcctggccgc ccatcccttc gtcgatccga aacgggtcgg ctgcatcggc
     8941 gcctcctacg gcggattcat gacgatgacc ctggcgacca agactccgat gttcgcggcg
     9001 gcaatttccc acgccgggat cagctcgatc tccagctatt ggggagaggg atactgggga
     9061 tacacgtaca gcgccgtggc cacggccggc agcttcccct ggaatcggaa agacatctat
     9121 gtcgaccgga gcccgctgtt ccacgccgac aagatcacga cgccgctgct tctcctccac
     9181 ggctcggccg acacgaacgt tccgccgggg gaaagcatcc agcttttcac cgccctcaag
     9241 cttctgggcc gggaaaccga gctcatctcg ttcgccgacg aaaaccatac gattatcacg
     9301 tacgacaaga ggattctctt caacaaaacc atcctggctt ggttcgaccg ctggctgaaa
     9361 gaccagccgg aatggtggtt cgatttgtat ccgcggaaat gaggagcgga cgtccgctcc
     9421 catttccaca gtttatgtgc ataatcttgt ggaaatcccc ggagggggtt tttctaaccc
     9481 cggtaaaaga aaacgattat gggaagttgc acaattttct gtcagcccga gcccggatga
     9541 aatttcttaa aataaacgcc atcttttaat ccttttggca cgttttttca tcccaagccg
     9601 cgggccggga gcggcaaaaa agcggggatc tcggccggcc ggtctcaaaa gacgtggccg
     9661 aagttgaaat agagccgcat tccttccttg ctgaacccga aatccacccg ggcgcagaaa
     9721 tctttcatga aaagacgcaa ccccacgccg taatcaaaaa ccgtcttgcg gaaattgata
     9781 tcgccgaagc tcggccagac gttgcccgca tcgatgaaga cgttgccgcc gaagatccac
     9841 cagacgggga accggtattc gacattggcc aggattttcc ccttgtccag gaaacgattg
     9901 aggccgaatc ccctcatgac attgaaggtg ttgccgccgc cgagggaaga caaatcaaac
     9961 aggggaatct cgtcgccgtt aatatattgg acgagtgtcc ggaaagcaaa cacgtccttt
    10021 tcgccgaaga cgcgctggta tttccgaaaa tcgaacgtga cccgattgaa ggaggcgtcg
    10081 ccgttgcggg aaaagctgac ggcgatgtcg tcttgaatca acagccggag cccccgggtc
    10141 ggatgaatct ggctgtcgga ggtgtcatac gccatggcca gggagaaata cggcgcggat
    10201 ttcttcttca gccgcctcaa ttctccggcc aaggggccgt cctggactcc ataataatcg
    10261 atcgtccgaa acacataggc caccgaggcg gaaaaatgcg gggtgaagcc gcggcccacc
    10321 gtcagaaaga gatttttcac ctcgtgggtg agaatcgtct cgtcttcctt gcgggaatcc
    10381 atcccctggc cgtagtaaga atatttcagg aacttgtcat attcggcctt gaggtcgaag
    10441 gagagaggat acgtcttccc ctgccggatc tcgatgtccg ggagggaaaa cgtaaaaaca
    10501 taccagcgct cccccttgga ggaattgaac aggatcaagt cgaaggattc cttgattccc
    10561 aggaaatcga cgagcttggc tttggccccg tagccgaacc cgatgtcgtt gtcatacatc
    10621 aggatcggaa gggcgctcca ggatttatag gatccgggtg cgatgcccgt gttctcggcg
    10681 gacgcgtcat ttgcggcgaa cgccagcgag gccggcagca atatcaacaa aagggccggt
    10741 atcaatgttt ttttcatgtt cttctccttc cccgatccgg atctactata tgagattccc
    10801 agggaatttc aagcttgccc gcggccgcgg caggctttga cgcagagtcc gcagatatgc
    10861 tggctgacca gccggatccg gcggaattca tcgagctttt cgaaacaagc ccgatgatcg
    10921 aattccgcgg gattcatttt aatggcttcg gccgggcaag aagccaggca ggcggcgcag
    10981 gtcccgcaat ccgccttcgt ctgaatcccg gcttccagcg gcatgtccgt cagaaccgtg
    11041 accagccgga gccgcgatcc gaaggcggga tgaaccaaaa gattgttgcg tccgatgtgt
    11101 cccagtcccg ccaggacggc gagctttttg tgggaaacgt gggccgtctg cctttgggaa
    11161 tcgaggatct gcgaggccgg tatcggcagg gcgagaaagc cggccgcctg gatgcgggcg
    11221 gcgagccgaa acgcctcccg gtccagaaaa aaattaagct ggcggtagtg gtggaaataa
    11281 aggggagtgg gacggtcgac gagatcctca aggacgccgt ccagcagccg gacgccgaga
    11341 accgccgccc ggacaaaccg gtttcgaagg ttttcgggca ggcggaatgt ctcccgaacg
    11401 gaagaaacgt cagccacccc ggcgacggaa aaaccaagat cccgggcggt ttccctgagc
    11461 cggtcggcgt tttccctggg agaaatggag aaaaacatgt gatcgtattc taggcgcggc
    11521 gccgggggac tgtcaatcga ctggaccggt ccaatggatc aggatcgcct tccccgtctt
    11581 tccagtgatc tccccgaagc atgagacgga agaaagcccg ccctctcccc gcccggtttc
    11641 aggggacggg gacgaagaca atcaagccga gggtcgccga ttgctcaaga cgaagacggc
    11701 ggccgatacg ctccgccgct cgacggcccg gccgggcccg ctctttccgc ccccggaggc
    11761 gcgtcagttg aaaagaatcg tctcctccgg ctcgcccagg aggatatatc cggcccagta
    11821 gaagggatgc ggccggccgc cgtggatcat ccccagcttc gccgcctgca acgcccggga
    11881 cttgccccgc ccggcttcca agtgacggta aaactccttc atgaagtccg ccgtcgatcg
    11941 gtccggcacc gcccaaagag tggctacgac ggaccgggcc cctccgtcca ggaaagcccg
    12001 gggcaagccc cccgcttctt tgggcggagc gtcgccgcac gcggtccggc atccggacaa
    12061 gacaacgaga tcggcggcca agcccagcgc ccgcgcctcc tcggctcgaa gcagaccgtc
    12121 atccccggcg cttcggcaaa gaagaagcga cgaacgcccc gggtcgcccg cctcggtcgt
    12181 tccatgcccg gcgatatgga caagcctgta gcggccgccg cggtgccgcc ggagagtcgc
    12241 ctcgctggcc gccccgtcca ggtaaacaaa acgacggttc cggtcgaaga atcgggaaac
    12301 gcttcggatt tcttcccgga cccccggcat ttcctcaaat acggcgtccg cttcgccggc
    12361 cccggccggg cggtccggga cggaagcgcc catggccagg agacaagagc cgccgggctc
    12421 ctcttccgaa cggtcgaggc ttggaccgaa tcggaaaggc gcgtaggaaa tcgcgtatct
    12481 ctcgaccagg aaccctcggc catcggccgt ccgaagcgcc tcgaacggga gacgacacag
    12541 gatgccgtcc gggatcacga ttaagcaatc gactcccagg gcggtcaatg tccggtcaaa
    12601 aggaatcaat tccccggcga ttcttccggc cgccgcggcc agcgaccgcc gtgaggccga
    12661 cggccgggct gtttccttga tgaaaccaag gacgctccgc tcaatgcgtc cggccgcggg
    12721 aagaacctcc gtccatgttc gatcttcgac ggaaatccta acccgggatt ccttttcgcc
    12781 gaggcggtat tccaagaccg cccggtttcc actgcctgga cggttgaagc ccgcgggagc
    12841 gctccccagt gcggaaaaga gccgctcggc cggtttccct cccgggcagc tcgaaaaacc
    12901 gagcgatagg aagcccgcca ggacgcccga aattccggcc ctcgctagtt ccgtgacctc
    12961 catattcatt ttctcgaaaa cgaacaatat catcaagtcg aaaaacgagg gggaaaatgg
    13021 agagcttcgg gggacaacgc ccgcggccgc tttcaacggt tccgggtccg gcgcgcggga
    13081 cggacggacg cccctgccgg ccgtctttcc atgagcatcc gcttctggtc cttgatttcc
    13141 tgaaatgtcc aggggacgta gacggattca cccgtccgcg gatcgcgccc cgtgtggtac
    13201 atcgccgtcg agagggtcat cggcgccggg gtgaagatct ggacgccttc aaccgggcgg
    13261 tccccttctt tttccagccg ccggacttcc cgggccagct ccccggcctc gcgggggccg
    13321 cagccgggat gggcgaccat gaaataatat ttcagatgcc ggcggccgga aacgtcggcc
    13381 ttccgaaagg cccgccggaa tttctcaaag gccccgccgg aatccttgtt catcaagcgg
    13441 aggacgtttt tcgaaacgtg ctccggggcg atcttgagaa ggcccgagac gtgatgcccg
    13501 acaagctcgc gg
//
LOCUS       DAOOXH010000067        12899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_4995_length_12899_cov_7.560262, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000067 DAOOXH010000000
VERSION     DAOOXH010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 12899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12899
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4995_length_12899_cov_7.560262"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PKV64_08290"
     CDS             complement(<1..647)
                     /locus_tag="PKV64_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17113.1"
                     /translation="MRPEFLILFVAYLALLLAVGLTFSRRMKSAEDFFFAGRRLSSGL
                     ICVSLTASWFGATSILVSVDEAFAGGVNAIWIVGVPAVATVLLLAALFASRLHRLPVL
                     TWSDLVELRYGRTVRHLASGLLVWYMAMLAASQMSALGLFLKDFLGVSYAGAIGAGAA
                     VVLIYTALGGLRSVIWTDVLQFLLLSAGVLALFFHIRGASSWTSAAVRAAAAGKNG"
     gene            706..1410
                     /locus_tag="PKV64_08295"
     CDS             706..1410
                     /locus_tag="PKV64_08295"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPN17114.1"
                     /translation="MRPGEGNFGGLDLWRTIAGAVFCLAGLLLPAWADEDAARDVFLR
                     SERALAALNSFQADFEQAYFSMTVSEPLREKGRLSYRKPGRMRWEYRSGSGQIIVLSD
                     GVMETLIPEENQLIRQIIPDDQADGALFGLLSGRTGLADAYRIENNPFPGAEGPVHQL
                     KLTPLEEGETAYLLVEIDARTFFLRRVVLFDWAGNKNEFTFSRLKTNPRLGPNVFVIE
                     VPDGCEIIDNVDPRRS"
     gene            1436..2023
                     /locus_tag="PKV64_08300"
     CDS             1436..2023
                     /locus_tag="PKV64_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPN17115.1"
                     /translation="MPEDKRSSGSDDPKLVRRCLRGDRKAFETIVLKYQQPMLNYVSR
                     MVREREMALDVSQEVFLRAYAALPSYRSQYKFSTWLFRIASNYLIDHWRKKKITAISL
                     DQPLDDTEDGCTLQVADETASIILEMERKELRLRLEKAMDRLPVHLKELFVWRHINGM
                     SYEEMAEIKKLPVGTVKNRVFQAKEMMRGLLEEMP"
     gene            2020..2619
                     /locus_tag="PKV64_08305"
     CDS             2020..2619
                     /locus_tag="PKV64_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HPN17116.1"
                     /translation="MSCPSVDRLYDDLDGALTGAEKEAVERHLQSCESCRRARAVRGR
                     IAGAAADLPPFEVPPDFAAGVMAGIAALPVPAAKKLHPGLVWTAAAAAAAGAGIGLGM
                     ILAGAGTAAWLSRIGPAFGAQLRNAAAFAAKGGKLLFLGGKIILGVSRGVLEFLRSTA
                     EILGPDGRAVLIGGTLVILISGGVFLRRRLPVSERTHEK"
     gene            2609..3589
                     /locus_tag="PKV64_08310"
     CDS             2609..3589
                     /locus_tag="PKV64_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17117.1"
                     /translation="MKNKILARIGAGLIVLAAAGLIPLPAFPQNSPIGYKKDILISPQ
                     SEQNQLFTWGGTVNIEGKVRKDVLAVGSEVTISGEVGDSFVGVGARVTLKPTAVIRKD
                     LLILGGTLVREEGSVVQGDTVYFKSEELRERFLQGGVGGLLGAAYLPIVLIIKIISLA
                     LWAVLAVIVAGLFPGNVTRAAGEMRRSLGTVLLTGLVASAAFLFLMVFAAVLSVILIG
                     IPVLIALSFAGILIKVFGRIVVYFTAGRLTAGAFRRTSVSPIGGALLGLAVVGVLRFI
                     PLVGGALSFFLSLLAWGIALRTKFGTTDNWFRKTAAPAPQAPLPPGPKQS"
     gene            complement(3694..4251)
                     /gene="frr"
                     /locus_tag="PKV64_08315"
     CDS             complement(3694..4251)
                     /gene="frr"
                     /locus_tag="PKV64_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPN17118.1"
                     /translation="MVKDVLQDCDRKMKASMEHFRKELSRLRTGRANLGVFEDIKVDY
                     YGVLTPVNQTARIGLPDPNLITIAPYDQSVLEAIDKAIRSADLGFNPINDGKLLKIPV
                     PPLDEERRREIVKKVKKMLEEEKTALRNLRREAKDFIEELEKEKEIGEDDKFKGLEEL
                     QKTIDDYTKKIEDIAAAKEKEIMQI"
     gene            complement(4266..4985)
                     /gene="pyrH"
                     /locus_tag="PKV64_08320"
     CDS             complement(4266..4985)
                     /gene="pyrH"
                     /locus_tag="PKV64_08320"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPN17119.1"
                     /translation="MPAPKPAYERVLLKLSGESLLGRQAFGIDFRTTQAIAQEIKEVH
                     ALGVQIAIMIGGGNIFRGVRGAAEGVERVSADQMGLLATIMNGIALQEALEKIGVMTR
                     HVSALEIRAIAEPFIRRRVLRHLEKGRVIIFSAGLGSPYFTTDTAAALRALEIKAGAI
                     LKATKVDGVYSADPLKDPKAKRFNRIRYIEVLKRGLEVMDATAISLCKDNKLPIIVFS
                     LKKRGNIQRVVLGEDIGTTVY"
     gene            complement(4997..5590)
                     /gene="tsf"
                     /locus_tag="PKV64_08325"
     CDS             complement(4997..5590)
                     /gene="tsf"
                     /locus_tag="PKV64_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPN17120.1"
                     /translation="MDITAEMVKELRERTGIGMMECKRALEEAGGDQEKAIEILRKKG
                     YARAADKADRATREGRIGSYIHTNGKIGVVVEVNCETDFVAKNEEFQELVKNIAMHIA
                     AAAPKYVAPADVPADVLEKEREIIREQFKDSKKPAEIVEKIVEGKLSKFYEETCLLEQ
                     PYIRDDKQKIKDLITQFISKFKENTKVGRFTRYQISK"
     gene            complement(5646..6410)
                     /gene="rpsB"
                     /locus_tag="PKV64_08330"
     CDS             complement(5646..6410)
                     /gene="rpsB"
                     /locus_tag="PKV64_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPN17121.1"
                     /translation="MVSVTMKELLEAGVHFGHQTKRWNPKMKEFIYGQRNGIYIIDLQ
                     KTIKVFKESLAFVKSVAESGKDILFVGTKKQAQDIVRDAAQKCESSYVNQRWLGGLLT
                     NFRVISGSIDKLVELEEMREDGRWDLLSKKEQSKLEKVYRKLSKNLGGIKNLKGIPGA
                     MFIIDSSRETIALEEARKMAIPIVAIVDTNGDPDGLTYPIPGNDDAVRAIELFSNKIA
                     EAILDGKKARIEKSMLEDKAAEEAAAAAEEPALDLG"
     gene            complement(6422..6826)
                     /gene="rpsI"
                     /locus_tag="PKV64_08335"
     CDS             complement(6422..6826)
                     /gene="rpsI"
                     /locus_tag="PKV64_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPN17122.1"
                     /translation="MSLIQYYGTGKRKTSIARVFLRSGKGDFTLFVNKRPRVFEDYFR
                     TESHRLKIKQPLALTSTEDKFDVFMRVEGGGVHGQSEAVRLGITRALIEYNRELKPAL
                     KAAGLVTRDSRIKERKKYGLLGARKAPQYHKR"
     gene            complement(6845..7273)
                     /gene="rplM"
                     /locus_tag="PKV64_08340"
     CDS             complement(6845..7273)
                     /gene="rplM"
                     /locus_tag="PKV64_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019999771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPN17123.1"
                     /translation="MKTYVPKKDAIERKWWVVDAEGKVLGRLATQVAVLLRGKAKPQY
                     APFVDTGDFVIVVNAEKIAVTGRKTELKKYHRHSGYPGGIKTEVLKDLLAKKPEEVIK
                     KAVWGMIPKNTLGRAVYKKLKVYRGPEHPHAAQNPQEYRF"
     gene            complement(7439..8431)
                     /locus_tag="PKV64_08345"
     CDS             complement(7439..8431)
                     /locus_tag="PKV64_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN17124.1"
                     /translation="MSRIRASRIIIGLFAVAIVVSTVACQKLNVNNLRANYYFNKGNA
                     LFNEKKYPKAIEEYEQAVKFNPNLKEAFRYIGESYKMIYAPGKDSDDNKLRGAKALNA
                     LVKAYEIDPDNKEIIYSLGDMYDKLHDIPSAEKMFLRIIELDPGNMNNYYIAAEFYKR
                     YAGENADLKTKAEQMYLRRIETDPDAVQGYAYMANYYDNLPTADFKDKFDRALDYHQK
                     RLSIEPQNAEVLYTIGVNRFNKAYQLQNFLSDFEKAEVAGEAEQALLKAIEIDPTFAN
                     SFAYMKMLYINIHAKVFPEKESRYLAEADRYGEKFKEAQARAIERMKLEKELKK"
     gene            complement(8631..9974)
                     /gene="der"
                     /locus_tag="PKV64_08350"
     CDS             complement(8631..9974)
                     /gene="der"
                     /locus_tag="PKV64_08350"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPN17125.1"
                     /translation="MSVETRSLPRVVIVGYPNAGKSTLFNRLLGRKKALVHSLPGMTR
                     DFIAGEARLGERRIELVDTGGFLDSPAEPFSVEVKAKAWAAAKSADVVILLLDGQRGL
                     LPVEEDLLRSLRKLGRPFVVAVNKIDTDAARPELAEYYRLGAPRLLFVSAEHKRGLGA
                     LEEAVASLLPPPREGEEDAGPRPLRIAVIGRTNVGKSSLVNRLCGEERAIVSEIPGTT
                     RDSTDVLIRRGSKSYLLVDTAGIRRFGKVADERESAGVIKARKNIPLADVLCLVLDVT
                     EFPARQDGTVAALALDSGKPLIIVLNKWDLVHEEDVVMEEVERALWSRLDFVNYAPLI
                     PVSALTGRHVTRILEEAERVFRNGRKMIGTAVLNKFVAVLNSEHPPMTRRGRRFSIKY
                     MTQKTVLPPTFAAFASVREPMAPAYEKYLIQRMRREFGFEGSPIRILLRGPKLKG"
     gene            complement(9991..10584)
                     /locus_tag="PKV64_08355"
     CDS             complement(9991..10584)
                     /locus_tag="PKV64_08355"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HPN17126.1"
                     /translation="MAKSARSILILVLAAAVAGLALIFFMSGNRESLKKIRPAEGPAA
                     EEETMPEDSRPKRTVRLLFVDEERESLIPEEREIVADAAAPAFEARQIIQELIRGSRT
                     GLLSPIPPETKLIQVYVAKDGTAYVDFSREFADRHPSGSSAEIATIYAVVDSLAINLK
                     SVRKVCLLVDGEERETLAGHIAISRPIPPDFSKIAGD"
     gene            complement(10592..11407)
                     /locus_tag="PKV64_08360"
     CDS             complement(10592..11407)
                     /locus_tag="PKV64_08360"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPN17127.1"
                     /translation="MKSRPPIVSGNFRPSSPVFPALVVLSALLFAASGTGFSAAPVAA
                     LRNIVIDPGHGGLDVGAKGRFGTHEKDVTLAVSLKLKALIEKTMSYRVIMTRDKDLDV
                     SLENRAAVANNNDAQLFISIHANGSVRNKARGAETFFMNVNASDEETRRLAYLENTSG
                     ELDKKIPAEAQDEVKMILWDMAQTAYIRQSQRLAEMIQEEINAALKLENRGIKQAGFK
                     VLQGVACPAVLVEMAFISNPEEERKLNDEFFQDHLAEALHRGLVRYLKLYPRK"
     gene            complement(11464..11811)
                     /gene="ssrA"
                     /locus_tag="PKV64_08365"
     tmRNA           complement(11464..11811)
                     /gene="ssrA"
                     /locus_tag="PKV64_08365"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11903..12640)
                     /locus_tag="PKV64_08370"
     CDS             complement(11903..12640)
                     /locus_tag="PKV64_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17128.1"
                     /translation="MSKARMVLSVFVVLALIAGLSAAGRGARNNGAADKPSGCVTIPM
                     GFVFYSPGHYLAGYPLAPGYDVFGYNFQARMFSGSYANAYLGGYGFPPYEGDDAAYLS
                     ENPAAAGVWCWPYREDNLIMKWNEAWLSTKDCDGDGKLDRHFGFPSYKGSGAWLTNHQ
                     SGVYPGGDGKEYSWNYFCKIAAVPADAVKTNGVWHTAGGVEIGPDIWGEFAVIQEVYN
                     DNGTGDHGLLYKSPAGPGFGKWDEVPQ"
BASE COUNT         2427 a         3907 c         3853 g         2712 t
ORIGIN      
        1 ccgttttttc cggccgccgc cgcccggacc gcggccgaag tccacgacga tgccccccgg
       61 atatggaaaa agagcgccag gacgccggcc gaaagaagca ggaattgaag aacgtcggtc
      121 cagatcaccg agcgcaggcc gcccaacgcc gtataaatca ggacgacagc cgcgccggcg
      181 ccgatggccc cggcataaga cacgccgaga aaatccttca ggaaaagccc cagggccgac
      241 atctgggaag cggccagcat ggccatgtac cagacgagaa gcccggaggc gagatgccgg
      301 accgttcggc cgtagcgaag ctcgaccagg tctgaccacg tcaagacggg aagacgatga
      361 aggcgggaag cgaacaaagc ggccaggaga aggaccgtcg ccactgccgg gacgccgacg
      421 atccagatcg cgttcacccc gccggcgaag gcctcgtcga cggataccaa aatcgaggtc
      481 gccccgaacc aggaggcggt cagggagacg cagatgaggc cgctcgaaag acgcctcccg
      541 gcgaaaaaaa agtcctcggc gcttttcatc cggcgggaaa aggtcaatcc gacggccaag
      601 agcagggcga ggtaagcgac gaacaggatt agaaattccg gacgcatgcc gagggttata
      661 ccagaaagat ccccggccga tcaaccgtgt tataataccg ggacgatgag accgggcgaa
      721 gggaatttcg gagggctcga cctgtggagg acaatcgcgg gagccgtttt ttgcctcgcc
      781 ggcctcctcc tccccgcctg ggcggacgaa gacgcggccc gggacgtctt cttaaggtcg
      841 gaacgggctc tggccgccct caactccttc caagccgatt tcgaacaggc atatttttcc
      901 atgaccgtgt ccgagccgct tcgggaaaag ggccgcctgt cctaccggaa acccggccgg
      961 atgcggtggg aataccggag cgggtccggg caaatcatcg ttctcagcga cggcgtcatg
     1021 gagacgctta tccccgagga aaaccagctt atccggcaga tcatccccga cgaccaagcc
     1081 gacggggcgt tgttcggcct tctttcggga cggaccgggc tggccgacgc ctaccggatc
     1141 gaaaacaatc ccttccccgg agccgaaggg cccgttcatc aactcaagct gactcccctc
     1201 gaggaggggg aaaccgccta tctcctcgtc gagatcgacg cccggacgtt ttttctccgc
     1261 cgggtcgttt tattcgactg ggccggcaac aagaacgaat tcactttttc ccgactgaaa
     1321 accaaccccc gtctcgggcc gaatgtcttt gtcatcgagg tcccggacgg ctgcgagatt
     1381 attgacaatg tcgatccccg gcggagctga actttttctc tcggagcccg tataaatgcc
     1441 tgaggataaa aggtcctccg ggtccgacga tcccaaactc gtccggcggt gcctgcgcgg
     1501 cgaccggaag gcgtttgaga cgatcgtcct gaaataccag cagccgatgc tcaactacgt
     1561 gagccggatg gtccgagaga gagagatggc cttggacgtc agccaggaag tgtttctccg
     1621 cgcttacgcg gctctcccgt cgtatcgttc gcagtataaa ttttcgacct ggctgttccg
     1681 gatcgcgtcc aattatttga tcgatcattg gagaaaaaag aaaatcaccg cgatctccct
     1741 ggatcagccg ctggacgaca ccgaggacgg ctgcactctc caagtcgccg atgagaccgc
     1801 gtccatcatc ctcgagatgg agcgcaagga gctccggctc aggctggaaa aggccatgga
     1861 ccggctgccg gttcatttga aagaactgtt cgtctggaga catatcaacg gcatgagtta
     1921 tgaagagatg gccgaaatca agaagctccc ggtcgggacg gtgaaaaacc gggtgtttca
     1981 ggccaaggaa atgatgcgcg ggctgcttga ggagatgcca tgagctgccc atccgtcgac
     2041 cgcctgtatg atgacctgga cggcgccctg accggggcgg agaaagaggc cgtcgagcgc
     2101 cacctccagt cctgcgaatc ttgccggcgc gcccgcgccg tccgcgggcg gatcgccgga
     2161 gcggccgcgg atcttcctcc atttgaggtt cctccggact tcgccgccgg cgtcatggcc
     2221 gggatcgccg cccttcccgt tccggccgcg aagaaactcc atcccggcct cgtctggacg
     2281 gcggcggccg ccgccgcggc cggcgcgggg atcggcctgg gcatgattct cgccggtgcc
     2341 ggaacggccg cctggctctc ccgaatcggt cccgcgttcg gagcccagct ccgcaacgcc
     2401 gccgccttcg cggccaaggg cgggaagctc ctcttcttgg gcggaaaaat catcctcgga
     2461 gtctcccgcg gagttctcga attcctgcga tcgacggccg aaatcctcgg gccggacggc
     2521 cgggccgttc tcatcggcgg aactctggtc atcttgattt ccggcggcgt attcctgcgc
     2581 cgccgactcc ccgtttcgga gagaacgcat gaaaaataag atcctggccc ggatcggcgc
     2641 cggcttgatc gtcctcgcgg cggcgggctt gattcccctg ccggccttcc cgcagaattc
     2701 gccgatcggc tataaaaaag acatcctgat cagcccgcag agcgaacaaa atcagctctt
     2761 cacctggggc gggaccgtga acatcgaagg caaagtccgc aaggacgtcc tggccgtggg
     2821 aagcgaagtg acgatcagcg gagaagtcgg cgattccttc gtcggcgtcg gcgcccgagt
     2881 gaccctgaag ccgacagccg ttatccggaa ggatctcctg atcctcggcg gaacgctcgt
     2941 ccgggaggaa ggaagcgtcg tccaagggga cacggtctac tttaaatccg aggagctgcg
     3001 ggagcgtttt ctccagggcg gcgtcggcgg actgctcggg gcggcgtatc tcccgatcgt
     3061 cctcatcatc aagatcatct ccttagccct ctgggccgtc ctcgcggtca tcgtcgccgg
     3121 tctgttcccc ggaaacgtga cccgggccgc cggcgaaatg cgccgctccc tcggaacggt
     3181 cctcttaacc ggcctcgtcg cctccgccgc gttccttttc ctgatggtct tcgccgccgt
     3241 tctcagcgtc atcctgatcg gcatccccgt cctgatcgcc ctctcgtttg cgggaatcct
     3301 catcaaggtc ttcggacgga tcgtagtcta cttcaccgcc ggccggctga ccgccggcgc
     3361 gttccgccgg acctcggtct cccccatcgg cggggcgctt ctcgggctgg ccgtcgtcgg
     3421 cgtcctccga ttcattcccc tcgtcggcgg agcgctgtcg tttttcctga gcctcctggc
     3481 ttggggcatc gccttgcgga cgaaattcgg gacgacggat aactggttcc ggaaaaccgc
     3541 ggcccccgcg ccccaagcgc cattgccgcc cgggccgaag caatcctgaa cgaggatctt
     3601 ggaaataccg aaattccccg ccgccttgac gccttcggaa atcagggaga agaagagcgc
     3661 ggatccgtcc gaggatgggg attccccggg aattcagatc tgcatgattt ctttttcttt
     3721 ggccgccgcg atgtcctcga ttttcttcgt gtaatcgtcg atcgttttct ggagctcctc
     3781 gaggcccttg aatttatcgt cctcgccgat ctccttttcc ttctcgagct cctcgatgaa
     3841 atccttggct tcacggcgga gattgcggag ggcggttttc tcctcctcga gcattttctt
     3901 gaccttcttg acgatctccc gccgccgctc ctcgtcgagg ggagggacgg ggatcttgag
     3961 gagctttcca tcgttgatgg gattgaagcc gagatcggcc gatcggatgg ccttgtcgat
     4021 cgcctccaaa acgctttgat cgtagggggc gatcgtgatc aggttcggat cgggaagacc
     4081 gatccgggcc gtctgattga ccggcgtcag aacgccgtaa taatccacct tgatgtcctc
     4141 aaaaacgccg agattggccc ggcccgtccg gaggcggctg agctccttgc ggaaatgctc
     4201 catggaggcc ttcatcttgc gatcgcaatc ctgtagaaca tccttcacca tgacggcctc
     4261 ctccttcaat acacggtcgt cccgatgtcc tcgcccagga cgacccgctg gatattgccc
     4321 cgctttttca agctgaaaac gatgatcgga agcttgttgt ccttgcagag cgagatcgcc
     4381 gtggcatcca tcacctcaag acccctcttg aggacttcga tataccggat ccggttgaac
     4441 cgcttggcct tcggatcctt gagcggatcg gccgaataaa cgccgtcgac cttggtcgct
     4501 ttgaggatgg ccccggcctt gatttccagg gcccggagcg cggccgcggt gtcggtcgtg
     4561 aaataggggc tgcccagacc ggcgctgaag atgatgaccc ggcccttttc cagatggcgg
     4621 agaacccggc ggcggatgaa cggctcggcg atggcccgga tctccagggc tgagacatgc
     4681 cgggtcatca ctccgatctt ctccaaggcc tcctggaggg cgatcccgtt catgatcgtg
     4741 gccagcaggc ccatctggtc ggccgagacg cgctccactc cctcggccgc tcctctcacg
     4801 ccgcggaaga tgttgccccc gccgatcatg atcgcgatct ggacgccgag agcatggact
     4861 tccttgattt cctgggcgat cgcctgggtc gtccggaaat cgattccaaa ggcttgcctt
     4921 cccaggagag actcccccga aagcttgagc agaacgcgtt cgtaggcggg cttgggcgcg
     4981 ggcatgcctc ctcccgttat ttcgaaatct ggtaccgggt gaaccggccg accttggtgt
     5041 tctccttgaa tttgctgatg aactgggtga tcagatcctt gatcttttgc ttatcgtccc
     5101 ggatgtacgg ctgctcgaga aggcaggttt cctcgtagaa cttggagagc ttgccctcga
     5161 cgattttctc gacgatctcg gccggttttt tggaatcctt gaactgctcg cggatgatct
     5221 cccgctcctt ttcgaggacg tcggccggca cgtcggccgg ggcgacatat ttcggcgccg
     5281 cggcggcgat gtgcatggcg atgttcttga cgagctcctg gaactcctca ttcttggcga
     5341 cgaagtcggt ttcacagttg acttcgacca cgacgccgat cttgccgttg gtgtggatgt
     5401 aggatccgat ccggccctcg cgggtcgccc ggtcggcctt gtccgcagcc cgggcataac
     5461 cctttttccg caggatctcg atcgcttttt cctggtcgcc gccggcttcc tcgagagccc
     5521 gcttgcactc catcatcccg atgccggtac gctcgcgcaa ttccttgacc atctccgccg
     5581 tgatatccat cgtgaactcc ttgtcgattt tcaaccccgg gcccgcgccc ggttcatccg
     5641 ccgactcaac cgagatcgag cgccggttcc tcggccgcgg ccgcggcttc ctccgcggcc
     5701 ttgtcctcga gcatcgattt ctcgatccgg gctttcttcc cgtcgagaat ggcctccgcg
     5761 atcttgttgg aaaacagctc gatcgcccgg acggcgtcgt cgttgccggg aatgggatac
     5821 gttaggccgt cggggtcgcc gttggtgtcg acgatggcca cgatcgggat cgccattttc
     5881 cgcgcctctt cgagggcgat cgtttcccgc gaggagtcga tgatgaacat cgctccggga
     5941 atgcccttta aattcttgat gccgcccaag ttcttgctca gcttgcggta gactttctcg
     6001 agcttggatt gttccttctt cgaaagaaga tcccagcggc cgtcttcccg catctcctcg
     6061 agctcgacca gcttatcgat cgatccgctg atgacccgga agttcgtcag gagcccgccc
     6121 agccagcgct ggttgacata gctcgattcg cacttctggg ccgcatcgcg gacgatatcc
     6181 tgggcctgct tcttggttcc gacgaacaag atgtccttgc cgctctcggc gacgcttttg
     6241 acgaaagcca ggctttcctt gaagaccttg atcgtcttct gcaagtcgat gatgtagatg
     6301 ccgttgcgct ggccgtagat gaattccttc atcttgggat tccagcgctt ggtttggtgc
     6361 ccgaagtgga cgcccgcctc caacagttcc ttcatcgtga ctgagaccac ggtttcctcc
     6421 gttaacgctt gtggtactgc ggggctttac gggcgccgag caggccgtat ttcttgcgtt
     6481 ccttgatgcg ggaatcgcgg gtgaccaggc cggccgcctt cagagccggc ttgagctccc
     6541 ggttatactc gatcagggcc cgggtgatcc ccagccgaac ggcttcggat tggccgtgga
     6601 cgccgcctcc ctcgacccgc atgaagacgt cgaatttgtc ctccgtcgag gtcagggcca
     6661 gcggctgctt gatcttcagc cggtgggatt ccgtccggaa ataatcctca aacacccggg
     6721 ggcgtttgtt gacgaacaag gtgaaatcgc ctttgccgga ccgcaggaag acccgggcga
     6781 tggatgtctt ccgcttgcct gtgccgtaat actgaatgag actcactgcg tcggcctcct
     6841 cgttttagaa ccggtattcc tggggattct gcgcggcgtg gggatgttcg ggtccccggt
     6901 aaaccttgag cttcttgtat accgcgcgcc cgagggtgtt cttcggaatc atcccccaga
     6961 cggctttctt aatgacttcc tcgggttttt tcgccaagag gtccttgaga acctcggttt
     7021 tgatgccgcc cggatacccg gaatgacgat ggtatttctt aagctccgtc ttccggccgg
     7081 tcacggcgat tttttcggcg ttgacgacaa tgacaaaatc gccggtgtcg acgaacgggg
     7141 catactgcgg cttcgctttt ccgcgaagaa gaacggcgac ctgcgtggcc aaacgtccca
     7201 ggactttgcc ttcggcgtcg accacccacc acttgcgttc gatggcatcc ttttttggga
     7261 cataagtctt catcggctga acctcaacaa gagatatgga aactctgact ccaggtttcg
     7321 ctaaaataaa atgatgcaat aagatactat ttccgggttc agatgtcaat gaaaaaaaac
     7381 cctcctcccc gacggagccg gagaggaggg cgggatgtcc tggaacaggc gaaaacgctt
     7441 actttttaag ctctttttcc agcttcatcc gttcgatcgc ccgagcctgg gcctctttga
     7501 acttttcgcc gtagcggtcg gcttcggcca ggtagcggct ctctttttcg gggaaaactt
     7561 tggcatgaat gttgatatag agcatcttca tgtaggcgaa ggaattggcg aaggtcggat
     7621 cgatttcgat cgccttgaga agagcctgct cggcttcgcc ggccacttcg gctttttcga
     7681 agtcggacag aaaattctgg agctgatacg ctttgttgaa ccggttgacg ccgatcgtgt
     7741 agagaacctc ggcgttttgg ggctcgatgc tcaggcgttt ctggtgatag tccagggccc
     7801 ggtcgaattt atccttgaaa tcggccgtcg gcaggttgtc gtagtagttg gccatataag
     7861 catagccctg gaccgcgtcg ggatcggttt cgatgcggcg aagatacatc tgctcggcct
     7921 tcgtcttgag atcggcgttc tcgccggcgt aacgcttgta gaactcggcc gcgatgtaat
     7981 agttgttcat gttgcccgga tcgagctcga tgatccggag gaacatcttc tccgcgctgg
     8041 ggatatcatg aagcttgtcg tacatgtccc ccagcgagta gatgatttcc ttgttgtccg
     8101 ggtcgatttc ataggccttg accagggcat tgagggcttt ggccccccgc agcttgttgt
     8161 catccgaatc cttgcccgga gcgtagatca tcttgtagct ttcgccgatg taccggaacg
     8221 cttccttcaa gttgggattg aacttgaccg cttgttcata ctcttcgatg gccttcggat
     8281 acttcttttc attgaacaga gcgtttccct tgttgaagta ataattggcc cgcagattgt
     8341 tgacgttcag cttctggcaa gccacagtgg aaacgacgat cgccacggca aagagtccaa
     8401 tgattatgcg cgacgcgcga atcctcgaca tcttgacctc ctgggacagc agtcccttga
     8461 tgggaataga ctccctttat aaacaaagac gcccggaaag tcaaatcacg ggggttttcc
     8521 gggggcggga ataaaaccga aaaaccgggc ttccgccgcc ccgcccgagg gcggaagccg
     8581 tttctcctcc gccgtccgag cggcggagca gccatcggtt cggcggaggg ctatcctttc
     8641 agctttgggc cgcggagaag aatccggatg gggcttccct cgaacccgaa ttcccgccgc
     8701 atcctctgga tcagatattt ttcataggcc ggggccatgg gctcccggac actggcgaag
     8761 gccgcgaagg tcggcggcag gacggttttt tgagtcatgt acttgatcga gaaccgccgc
     8821 ccccgccggg tcatcggggg atgttcggaa ttcaggacgg cgacgaactt gttgagaacg
     8881 gccgtgccga tcatcttccg gccgttgcgg aaaacgcgct cagcctcctc caggatccgg
     8941 gtcacgtgcc gaccggtcag ggcggaaacc ggaatcaaag gagcgtaatt cacgaaatca
     9001 agccggctcc aaagcgcccg ctcgacctcc tccatgacca cgtcctcctc gtggaccagg
     9061 tcccatttgt tcaggacgat gatcagcggt ttccctgaat ccagggcgag ggcggcgacc
     9121 gtcccgtcct gccgggccgg gaactcggtc acatccagga ccaggcagag gacgtcggcc
     9181 aagggaatgt ttttccgggc cttgatcacg ccggcgcttt cccgctcgtc ggcgaccttg
     9241 ccgaaccggc ggatgccggc cgtgtcgaca agaagatagg atttcgagcc tcgacggatc
     9301 aaaacgtccg tgctgtcccg ggtcgttccg gggatttcgc tgacgatggc cctctcctcc
     9361 ccgcagagcc ggttgaccag ggaggatttt ccgacgttgg tccggccgat cacggcgatc
     9421 cgcagcggcc gggggccggc gtcctcctcc ccttcccgcg gcggcgggag gagggaggcg
     9481 acggcttctt ccagggcgcc gaggccgcgc ttgtgctcgg ccgatacgaa cagaagacgc
     9541 ggcgcgccca gccggtaata ttcggccagc tccggtctcg ccgcgtccgt gtcgatcttg
     9601 ttgaccgcca cgacaaacgg ccggcccagc ttccggagcg accggagcaa gtcctcctcg
     9661 accggcagaa ggccccgctg tccgtccagg agaagaatga cgacgtcggc cgacttcgcc
     9721 gcggcccaag ccttggcctt gacctcgacg gaaaagggct cggccgggga atcgagaaag
     9781 ccgcccgtgt ccacgagttc gatccggcgt tctcccaggc gggcttcgcc ggcgatgaaa
     9841 tcccgggtca tcccgggcag cgaatggacc agcgcttttt ttcggccgag aaggcggttg
     9901 aagagcgtcg atttgccggc gttgggatag ccgacgatca cgacacgggg aagggatctc
     9961 gtttcaacgg acatggccgg cgccttccgg tcaatctccg gcgatcttgg agaaatcggg
    10021 ggggatcgga cgactgatgg cgatgtgccc ggccagcgtc tcccgttcct cgccgtcgac
    10081 caggagacag accttccgga cggatttcaa attgatcgcg agggaatcga cgacggcgta
    10141 gatcgtcgcg atctcggcgc tcgatccgga cggatgccgg tcggcgaact cgcgggaaaa
    10201 atcgacataa gcggtcccgt ccttggccac atagacttga ataagcttgg tttcgggcgg
    10261 gatgggggaa agaagccccg tccgggatcc ccggatgagc tcctggatga tctggcgggc
    10321 ctcgaaggcc ggggcggcgg cgtcggcgac gatctccctt tcctcgggaa tcagggattc
    10381 gcgttcctcg tcgacaaaga ggagccgcac cgtccgtttc ggccgcgaat cctccggcat
    10441 cgtctcttcc tcggccgccg ggccctcggc cggccggatt tttttgaggg attcccggtt
    10501 gccgctcatg aagaaaatga gggccagtcc ggccacggcc gcggctaaaa ccagaatcag
    10561 gatggaacga gcggatttgg ccatcggcgg gttatttccg gggatagagc ttcaggtagc
    10621 ggacgagccc ccggtgaaga gcctcggcaa ggtgatcctg aaagaattcg tcgttgagct
    10681 tccgctcctc ctcgggattc gagatgaagg ccatctcgac caggacggcc ggacaggcca
    10741 ctccctggag aaccttgaag ccggcttgtt tgattccccg gttctcgagc ttgagggccg
    10801 cgttgatctc ctcctggatc atttcggcca gccgctggct ctgccggata taggccgtct
    10861 gggccatatc ccaaaggatc attttgactt cgtcctgggc ctcggccgga atttttttgt
    10921 ccagctcgcc gctcgtgttt tcgaggtagg ccagcctccg cgtctcctcg tccgaggcgt
    10981 tgacgttcat gaagaacgtc tccgcgccgc gggccttgtt ccggaccgat ccgttggcgt
    11041 ggatgctgat gaagagctgg gcgtcgttgt tgttggccac ggccgcccgg ttttcaaggg
    11101 agacgtcgag atccttgtcc cgggtcatga tgacgcggta ggacatggtt ttttcaatca
    11161 aagccttgag cttgaggctc acggccaggg tgacatcctt ctcatgggtc ccgaaccggc
    11221 ccttggctcc gacgtcgagc ccgccgtggc ccggatcgat gacgatattg cgcagggccg
    11281 cgaccggagc ggcggaaaaa ccggttccgg aagcggcgaa aagcaaggcg gacaggacga
    11341 caagcgcggg gaagacggga gaagaaggcc tgaaatttcc agaaacgatc ggcggtcgag
    11401 atttcatatt tctcatcccg cccgcatttt aacaagaaac aacgcccgaa accagagaag
    11461 aggtggtgga ggcggcggga atcgaacccg cgtccgaaga cattcagcgg gaagagtcta
    11521 catgcttatc ctcttctttc atctcgcctc gcggttcccg aagaggaagg ggccgctcgg
    11581 ccagcctcga tgttgtttcg ctccccgggc tcaaggccgg cccggctcgc tatcccacgg
    11641 gttggcgctc cggggacccg gtgggagcgg gtccggggaa cggccgccta actaattaag
    11701 cagcgagagg aagtgtttct gcacttactt gggttccacc gtttaacgag agagatggga
    11761 tctcggcatg cctctttcgc ctcattatct ccgtcgaatc catacgcccc ctttccaaag
    11821 attttgggac actctcagta taggggcggg agggcgggaa gtcaagaaaa acccacctta
    11881 aaaatccgct aacgccctca tttcattggg gaacctcgtc ccatttgccg aaaccgggac
    11941 cggccgggct tttatagagg aggccgtgat cccccgttcc gttgtcgttg tacacttcct
    12001 ggatgacggc gaattcgccc cagatgtcgg gaccgatctc gacgccgccg gcggtgtgcc
    12061 agacgccgtt cgtcttgacc gcgtcggccg gaacggccgc gatcttgcag aaataattcc
    12121 agctgtattc cttgccgtcg ccgccgggat acacgccgga ctgatggttc gtcagccagg
    12181 cgcccgatcc tttgtaggaa ggaaatccga aatgccggtc gagtttgccg tcgccgtcgc
    12241 agtccttggt ggagagccag gcctcgttcc atttcatgat caggttgtct tcgcggtacg
    12301 gccaacacca aacgccggcc gccgcgggat tttccgacaa gtaggcggcg tcgtccccct
    12361 catagggagg gaagccatag cctcccaaat aagcattggc ataggaaccg ctgaacatgc
    12421 gggcctggaa gttgtatccg aacacatcat atcccggagc gagggggtat cccgcaagat
    12481 aatgtcccgg agagtagaag acgaacccca tggggatcgt cacgcaaccg gacggcttgt
    12541 ccgccgcccc gttgtttcgg gccccgcggc cggccgcgga caggccggcg atcagggcca
    12601 ggaccacgaa aaccgacaaa accatgcgcg ccttgctcat cgatccctcc tttatggata
    12661 tcgaattttt cgaaattcga gaaaaaaaaa gaatgaagga atggaaaaac tcccttggac
    12721 ccttttccgg aggcgacatt cgagcccccc caacaaggcg atgatcacac ctgacttgtt
    12781 tctcaacata ttcctctttt ttttctaagt caatatgtgt tttcacagga cttaccccaa
    12841 aaacattttc atttttggaa gaggagtcgc tggaaggata tcggcggggg aaggcgagg
//
LOCUS       DAOOXH010000068        12839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_5029_length_12839_cov_7.781602, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000068 DAOOXH010000000
VERSION     DAOOXH010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 12839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12839
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5029_length_12839_cov_7.781602"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..793
                     /locus_tag="PKV64_08375"
     CDS             50..793
                     /locus_tag="PKV64_08375"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPN17129.1"
                     /translation="MTEKKLSVQDVMNMTRDKCEGTVFDPARGLQGGPFSNPNFLPYG
                     FKLDDKAYNTPRIIGVNRAEYVTVTQARKGLPDPIGGIVWLAMGFQDTSCFMPLYNGI
                     TEIPRGLQIGDHFEFSREATRWAFDYVDFHTQVVYSHAIQDVRAAQQKYEAPAVARTP
                     MVDKYALELYQKDPAAAAQYLTDYCLNNIAAVTDAWWKLGDMLMVKYNKLWIYDVKAR
                     KRAPLLFPDWYLRLLVEQNKLAPKETDEH"
     gene            869..1507
                     /locus_tag="PKV64_08380"
     CDS             869..1507
                     /locus_tag="PKV64_08380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HPN17130.1"
                     /translation="MRGRWMQAAAWLLLSAAGAAQTPPASSAPPGPLERPVLLIIDVQ
                     NFYFEGGTLPLAGSVKASLQAKTLLESFRAKNLPVIHVAHMPKEKPAAPDPLYSFHPN
                     VAPRPGEAVVIKHYADAFRETDLLDRLRALRAETLVICGMQTHMCVEAAVRHAADTGF
                     RVVLAEDACATRDLKYKRTTVPAASVQAAVLAALNGTYAKIAAAAEIAAALK"
     gene            1512..2762
                     /locus_tag="PKV64_08385"
     CDS             1512..2762
                     /locus_tag="PKV64_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN17131.1"
                     /translation="MKPPAKLFGWLRDAPPASRRSLLAASLGWMLDSFDVMLYSMVLA
                     YLMKDLGMSKATAGLMGSITLVASAAGGVIFGLVADRMGRTLALRASILIYSIFTAAC
                     GLASSVPMLAVFRVFLGLGMGGEWASGAALVSETWPAEHRGKALGVMQSSWAVGYALA
                     ALVTAVIYPWLGWRAVFFFGVLPALLVFWIQTKVEEPAIWVEHREREAKAETARPSIR
                     SIIRGRLLGTTVLITIMNAATLFGWWGLNLWIPGYLSLSRDQGGIGLSPRHMSSLVIF
                     MQAGMFLGYLTYGYISDRLGRKRTYIAFLLCAAVLIIAYSGVRTPTALLFLGPFVAFF
                     GTGYFSGFGALSAELYPTSIRATLQGFTYNIGRVVSAVAPLVIGSLAQTKGFGTAFLL
                     VAAAFLFAAAMWIWIPETRGRALA"
     gene            3009..4475
                     /locus_tag="PKV64_08390"
     CDS             3009..4475
                     /locus_tag="PKV64_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPN17132.1"
                     /translation="MRGTRSLAAVLIGLSLVLAPLRGRAETDPLKEAWNRLPAAEKHF
                     QKGLREFQAGRYAAAAGAFRKTIDALPRHAYASYYLANIAYVQGDFQNALADMERSLR
                     DLDFMRDLNTYAVAKQSQTIDSYRQMLDSEWENAPDCRSQREIESLTDEIATEKNKLE
                     VRTAAEDASRLSQRAHFLYFLGNIRFQLKQFPEALDAYQKALDLNPRHVSAVNNAAAV
                     RYMAGDPQGALDQLDEGARAGVGGHLNLKLKHLILEALGRPTEGVLQEDLSAAGPGAD
                     LGVVRFALAFNGGDPLLPPLYENGYVVFSRTSRQAILVDPGVEDPRIAELIARNHLEV
                     KAVFNTHNHDDHAAADKVYAALYKAPVYLSPKEEKRTDRPAVRPIGDGETISFEGFTV
                     QALLTPGHTAGSTCFLIGGYLFSGDTLLADDIGSPAGGNNRQRADARSGLVRVIREKL
                     LSLDGRTRVCPGHGKTTTIAAEAAGNPFLKNQPADLNR"
     gene            complement(4451..5173)
                     /locus_tag="PKV64_08395"
     CDS             complement(4451..5173)
                     /locus_tag="PKV64_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPN17133.1"
                     /translation="MTVPEIPGKPRPYDDRSTETGIILYRYRGHNPSHPDNAGLRKAM
                     NRGIPLIYFYGVEPGWYEPIWPVFIVGDDPEKLFFSVIADDQKAIVKERSNPPSVEDF
                     HRRYITVEIQRRIHQNAFRARVLRAYQYSCAICRLRHHELLEAAHILEDKHPSGIPAV
                     NNGLAMCAIHHKAFDSDILGIRPDYYVEIRDDVLQEKDGPMLLHGLQGFQGKILNLPR
                     RMEERPDRERLEERYQRFRSAG"
     gene            complement(5374..6204)
                     /locus_tag="PKV64_08400"
     CDS             complement(5374..6204)
                     /locus_tag="PKV64_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPN17134.1"
                     /translation="MFLFKLHRSQRTRQGDVIAGGGVFSSFSTLPISLAWEAFDIRNG
                     ASSFSDMRRRLLPYRRIENDPHEDFKIGCIILTQPFFFEESMWFPAPEWKASIVRGKA
                     KGYELGTDAGRFIWKNVQEGLARQGLSDLYLDTRRVEEERARYGKESVIRPRLGQGAF
                     QVAVTDAYARSCAITEEHSLPALEAAHIKPYGEEGPHEVSNGLLLRSDFHRLFDRGYI
                     TVTPEFRIEISRRLKEEFANGRSYYPFHGRRLNHYPQTEEDRPSRQMLLWHNENRFLG
                     "
     gene            6466..6984
                     /locus_tag="PKV64_08405"
     CDS             6466..6984
                     /locus_tag="PKV64_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17135.1"
                     /translation="MKKPVLIAAVAAAVLLTASPLLRGSETGPEATVAKIRDRILAPD
                     FKPDGIEKDLIEILNVSLAILPEKEYAEEFRSKIAWVKDSFADEHLFADKIRQYLGIA
                     YALVSDGTSWRLPEELQRPYPGEPASIKRATAVCGRLLESALAEMKAGNNEKAAGCLI
                     GFVLLVITPIEA"
     gene            7069..7617
                     /locus_tag="PKV64_08410"
     CDS             7069..7617
                     /locus_tag="PKV64_08410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPN17136.1"
                     /translation="MGNPAFDYDSLIKPLEPRMMRTIWRILREKPAAEDALQDALAVI
                     WKKRGTVARHPNPPALILRITIASAVDSLRKTRRRLRHEVPGLPKDRRADDSADPAAG
                     KAEERRFRPAILQAVGRLPRRQAEAVLLRIIEELSYEEIARAMGCTEATARVHVMRGK
                     AALARRLARFRPDPARQPGLKE"
     gene            7634..7990
                     /locus_tag="PKV64_08415"
     CDS             7634..7990
                     /locus_tag="PKV64_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17137.1"
                     /translation="MRAKRFEDADLERLLRDALPDDLPDEAAAGMRDRIAAFRTAAAK
                     EERGSAVSFILARKGAWAVLALALLIAGALLQGFQSPSPLAKKIAFLKNELSNSEPAR
                     PADIDGGIRIPGLKEG"
     gene            7993..9000
                     /locus_tag="PKV64_08420"
     CDS             7993..9000
                     /locus_tag="PKV64_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17138.1"
                     /translation="MNEHDKNRVRGRAAVRWLIAAAFIAGVALSGLFAGMANRAGLFF
                     LFLGTAASVLTGFSRSEIREAFRLAAGKSGTPEARREAASFWEAAARNAWALGVMGSL
                     LNFTVVLGGGSGGIADISDRMIQSFIVTFYGLVLAVLLLVPAMKISGLAGNGEPGPGP
                     RPSRGGPFLFERVTGYVLFAVVLGLTVLSLVRSVPPGGPLPAAEVMLHGPAALFIFGG
                     SIALALFLGTGRGAWTFGFGMTGLAALFLGLIQTMFGFVHTDIKEISAAIAFIISSSL
                     YALLGLVLVAAPLEDRKRPDGGDQPGRLSRVLWMAFPFVAFIFLVLSWILVITPMKRT
                     G"
     gene            9093..10034
                     /locus_tag="PKV64_08425"
     CDS             9093..10034
                     /locus_tag="PKV64_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPN17139.1"
                     /translation="MKRRDFVSKGLGTAAAVSLPFLGTAGRPFSAGRESAPPPDGGTS
                     RFKLGYAPPFGLFKAHAGDDPAAQIRFCADEGFTAMFDNGLMGRPAAQQELISRELAA
                     RGMKLGPFVLYSDFGVKSFVLRDKDVRAMLVERMKKGVETMKRTGCAQALVVPGRFDE
                     SLAWEYQTANVVDNLRACLDVCRPAGLVLVLEPLNPKDHPGLFLTRIPQAYALCRAVD
                     DPHCKILDDLYHQQITEGNLIPNIEAAWSEIAAFHVGDSPGRKEPTTGEINYRNIFKW
                     IHGKGYQGVLCLEHGLSRPGKEGERAVIEAYRACDAF"
     gene            10071..11339
                     /locus_tag="PKV64_08430"
     CDS             10071..11339
                     /locus_tag="PKV64_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN17140.1"
                     /translation="MSLSKNITRRDFIKTTAAASLAAAIPGNLGLFAAGSDALRIGVI
                     GCGGRGTGAAINCLEAAEGIEIVALGDLVPDRIESSYRKLMENFPDRVRVPAGNRFTG
                     FDNHLKVCALPEVNLIVTAAPPGFRPIHLKAAVEAGKHVFMEKPVAVDPAGVRAVIAS
                     SEAAAAKGLAVVAGTQRRHQKSYLELMRRIRDGQIGEIVGAQCYWNQGDLWVKLPEPG
                     MSEMEWQCRNWLYFTWTSGDHIVEQHVHNIDVVNWAIGALPKNIVGMGGREVRKAPEY
                     GNIYDHFAVEFEYPNGVRAASYCRQTEGCADRVEERIIGTKGTAFGYGEIKGEKPWTF
                     EGPEPDPYVQEHADLVASIRAGTPLNEGRRIAESTMCAIIGRMSAYTGRAISWDWAMN
                     ASKLDLSPARYEFGPNPVDPVAVPGKTPLI"
     gene            complement(11365..12072)
                     /locus_tag="PKV64_08435"
     CDS             complement(11365..12072)
                     /locus_tag="PKV64_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPN17141.1"
                     /translation="MKKMPFALCLLLLAAPAFPYQVEKSLTLPAEGIRALEVNAGAGS
                     LRIEGVEGLSSIEVDAEIIIRGAGKDRVEDFMDNHLRFSLEKIGERAVLKGYFQFDGF
                     PRRFSGDALCNLTVRVPKTIDLAVDDGSGSMAVVNIRGDVSIEDGSGDITVRGVEGDV
                     RVDDGSGDIDIRDVTGGLRIDDGSGSMTITGIGGSVTVDDGSGDIEIDDVGGDVILES
                     TGSGGVSIGFVRGRVIR"
     gene            12216..>12839
                     /locus_tag="PKV64_08440"
     CDS             12216..>12839
                     /locus_tag="PKV64_08440"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HPN17142.1"
                     /translation="MPKTSDSTRNERQEALKGIIKDLHAGATVKDLRKRFAVLIKDTS
                     AEEIADMENALIQEGFPVEEIQRLCEVHAEVFDKSLRKAGKPSKIPGHPVHTYLEENR
                     EARRRLKDMKRALKKIKENRPGDREIEAFRERFEAFREIEKHYARKENQLFPPLEATG
                     FTGPTKVMWGKHDEIRAHLKSAADLLAAGDWAGLKDRFQTLASAVKKM"
BASE COUNT         2392 a         4247 c         3830 g         2370 t
ORIGIN      
        1 ggcgtaggtg ggggaggtgt agttctggac ggaaagcttc ttctcgggct tgaccgagaa
       61 gaagctttcc gtccaggacg tcatgaacat gacccgggac aagtgcgaag gcacggtctt
      121 cgacccggcc cgcggcctgc agggcggacc cttctccaac ccgaatttcc tcccctacgg
      181 cttcaagctg gacgacaagg cttacaacac gccgcggatc atcggcgtca accgggccga
      241 gtacgtgacc gtgacccagg cccggaaggg cttgcccgac ccgatcggcg gaatcgtctg
      301 gctggccatg ggcttccagg atacctcctg cttcatgccc ctttacaacg gcatcaccga
      361 gattccccgc gggctccaga tcggcgatca tttcgagttc agccgcgagg cgacccgctg
      421 ggcctttgat tacgtggact tccacaccca ggtcgtgtac tcccacgcca tccaggacgt
      481 ccgggccgcc cagcagaagt acgaggcgcc ggccgtcgcc cggaccccca tggtcgacaa
      541 gtacgccctg gagctctacc agaaagaccc ggccgcggcc gcccaatacc tgaccgatta
      601 ctgcctgaac aacatcgccg ccgtgaccga cgcttggtgg aagctgggcg atatgctcat
      661 ggtcaaatac aacaagctct ggatctacga cgtgaaggcc cggaagcgcg ctcccttgct
      721 cttccccgac tggtatctcc gccttctggt cgagcagaac aagctcgccc cgaaggagac
      781 cgacgaacac tgatcccggc ggagatccga agatttccga tccccgccct ccattcggag
      841 ggcggggatt ttttttagga gggcgtccat gagaggacga tggatgcagg cggcggcctg
      901 gcttcttcta tcagccgccg gagcggccca aaccccgccg gcgtcctccg cgcctcccgg
      961 cccgctcgag cggccggttc tgttgatcat cgatgtccag aacttctatt tcgagggcgg
     1021 aacgctcccc ctggccggaa gcgtcaaggc ctcgcttcaa gcaaagacgc ttttagagtc
     1081 gttccgggcg aaaaacctcc cggtgatcca tgtcgcccat atgcccaagg aaaaaccggc
     1141 cgcccccgac ccgctgtact ctttccatcc caacgtcgcc ccgcggcccg gcgaggctgt
     1201 cgtcatcaaa cactacgccg acgccttccg ggaaacggat ctcctggacc gtctccgggc
     1261 gctgcgggcc gaaaccctcg tcatctgcgg gatgcagacg cacatgtgcg tcgaagccgc
     1321 cgtccggcat gccgccgaca ccggcttccg ggtcgtcctg gccgaggatg cctgcgcgac
     1381 gcgcgacttg aaatacaagc gcacgacggt cccggccgcc tcggtccagg cggccgtcct
     1441 ggcggccctg aacggcacgt acgcgaaaat cgccgcggcc gccgagatcg cggccgccct
     1501 caagtgaccg gatgaagccc ccggcgaagc ttttcggctg gctgcgcgac gccccgcccg
     1561 cctcgcgccg gtcgctgctg gccgcctcgc tggggtggat gctcgattcc ttcgatgtca
     1621 tgctctactc catggtcctg gcttacttga tgaaggacct gggcatgagc aaggccacgg
     1681 ccggattgat gggctcgatc accctggtcg cctcggccgc cggcggggtg attttcggcc
     1741 tcgtggccga ccggatgggc cggacgctcg ccctccgggc tagcatcttg atctactcca
     1801 ttttcaccgc ggcctgcggc ctggcctcgt ccgttccgat gctggccgtt ttccgggtgt
     1861 ttctcggcct gggcatgggc ggggaatggg ccagcggggc ggctctcgtt tccgaaacct
     1921 ggccggccga gcaccggggg aaagccctcg gcgtcatgca gagctcctgg gccgtcggct
     1981 acgccctggc cgcgctggtc acggccgtca tctacccttg gctcggctgg cgggccgtct
     2041 ttttcttcgg ggttctcccg gccctcctgg tgttctggat tcagacgaag gtcgaagagc
     2101 cggccatctg ggtggagcac cgggagcggg aagcgaaggc cgagaccgcg cggccgtcca
     2161 tccgctccat catccggggc cgcctcctgg gaacgaccgt cctgatcacg atcatgaacg
     2221 cggccactct gttcggctgg tgggggctca acctctggat ccccggctac ctctctcttt
     2281 cccgggacca gggcggaatc ggcctgtctc cccggcacat gtcgtccctg gtcatcttca
     2341 tgcaggccgg gatgttcctg ggctacctga cgtacgggta catcagcgac cggctcgggc
     2401 ggaagcggac gtacatcgcc ttcctcctct gcgccgccgt cctcatcatc gcctactcgg
     2461 gggttcggac gccgacagcc ctgttgttcc tgggcccctt cgtggccttc ttcgggacgg
     2521 gctacttttc cggattcggc gccctttcgg ccgagctcta cccgacgtcc atccgggcga
     2581 cgctccaggg atttacttac aatatcggcc gagtcgtctc ggccgtggcc cccttggtca
     2641 tcggctcgct ggcccagacg aagggcttcg gaacggcctt cctgctggtc gccgcggcct
     2701 tcctcttcgc cgcggccatg tggatctgga ttcccgaaac gcgcggccgg gcgctggctt
     2761 aggccgccct ttttgtgcgt tctaattcgt tctcctcaac caagaaaaga agaccggcga
     2821 tggaaaaccg cccggaaagg cgggcctttt ctctcgaagg ccgttttttt acgttcaagt
     2881 ctgattcgtt acattccagg gccctgggaa aactccccgg cagacggctt ttcttcgtta
     2941 tttctcggga aggccgattg gcacggcgct tgctgtttca atcttggaag aaaaggaata
     3001 atccgaacat gcgcggaacg agatctctcg ccgccgtcct gatcggcctg tccctcgtcc
     3061 ttgcgcctct ccgggggcgg gccgagaccg atcccctcaa ggaggcctgg aaccgcctgc
     3121 ccgcggccga gaagcatttc cagaaaggac tgcgggaatt ccaggccggc cggtacgccg
     3181 cggccgcggg ggcgttccgg aaaaccatcg atgctctccc ccggcacgcc tatgcctcct
     3241 actacctggc caacatcgcc tatgtccagg gggactttca aaacgccttg gccgacatgg
     3301 agcggtcgct tcgagacctg gacttcatgc gggatctcaa cacttatgcc gtcgccaagc
     3361 aaagtcagac gatcgattcg taccggcaga tgctcgactc tgaatgggag aacgcccccg
     3421 actgccggtc ccagcgcgaa atcgaatctc tgaccgacga gatcgccacc gagaaaaaca
     3481 agctggaggt ccggacggcc gccgaggacg cctcccgttt gtcccagcgc gcccatttcc
     3541 tttatttcct gggcaatatc cgcttccagc tgaagcaatt ccccgaggcc ttggacgctt
     3601 accaaaaagc gctcgacctc aatccccgcc acgtcagcgc ggtcaacaac gcggccgcgg
     3661 tccggtacat ggccggcgac ccgcagggcg ccctggacca gctggacgag ggagcccggg
     3721 cgggcgtggg cggccatctg aacttgaagc tcaagcatct catcctcgaa gccctgggtc
     3781 ggccgacgga aggcgttctc caagaggatc tctcggccgc cggtcccggg gcggacctgg
     3841 gcgtcgtccg gttcgctctc gccttcaacg gcggagaccc gcttttaccg cccctctacg
     3901 agaacggtta cgtcgtgttc agccggacgt cccggcaggc gatcctcgtc gacccggggg
     3961 tcgaggatcc ccgcatcgcc gagttgatcg cccggaacca tctcgaggtc aaagccgtct
     4021 tcaacacgca caaccacgac gaccacgcgg ccgccgacaa ggtttacgcc gccctctaca
     4081 aagccccggt ctatctttct cccaaggaag agaaacggac ggaccgcccg gcggtccgtc
     4141 ccatcggcga cggcgaaacc atctctttcg aaggattcac ggtccaggcc ctgctcacgc
     4201 ccggccacac ggccggaagc acgtgctttt taatcggggg atatctcttc tcgggcgaca
     4261 ccctgctggc ggatgatatc gggtctccgg ccggcggcaa caaccggcag cgggcggacg
     4321 cccggagcgg cctggtccgg gtcatccgag agaagcttct gtccctggac ggccggacgc
     4381 gcgtctgtcc cggccacgga aagacgacga ccatcgccgc cgaagcggcc ggcaatccat
     4441 ttttaaagaa tcagcccgcc gatctgaatc gctgataacg ctcttccagg cgttcccggt
     4501 ccggccgctc ctccatccgc cgcgggagat tcaggatctt cccttgaaat ccctgcaacc
     4561 cgtggaggag catggggccg tccttttcct ggagaacgtc atccctgatt tccacgtaat
     4621 aatccggtcg aatccctaaa atatcgctgt cgaaagcttt atgatgaatc gcgcacatgg
     4681 ccagaccgtt attcacggcc gggataccgc ttggatgctt gtcttccaag atatgcgccg
     4741 cttcaagaag ctcatgatgc ctcaaccggc atatcgcgca cgaatactga taggctctta
     4801 ggacgcgggc gcgaaaggcg ttttgatgaa tcctccgctg gatctcgacc gtgatataac
     4861 gccgatggaa atcttccacg gaaggaggat ttgatctttc ttttacgatc gccttctggt
     4921 catcggcgat gacgctgaaa aacagctttt ccgggtcatc gccgacaatg aataccggcc
     4981 aaatcggttc ataccatccc ggttccactc cataaaaata aatgagcgga attcctctat
     5041 tcatcgcctt ccgcaacccc gcattatccg gatgcgaggg attgtgcccg cggtatcgat
     5101 agagaatgat cccggtttcc gtcgaccggt cgtcgtaggg acgcggtttt cccgggatct
     5161 ccggaaccgt caagatgctg atcgggattt cagggagaat ttttggcttg aagattccct
     5221 gcggtccgac aaggggaacg cggacaccct caaaattgaa tcccttcgcg aggacgttcc
     5281 gcgggatcgc ccggccgtag tgacgaagtt gttcgattaa aaaggcggaa gccgcctggc
     5341 ggacttttcg atcgagatca atctctctat agatcatccg agaaacctgt tttcattatg
     5401 ccagagaagc atctgtcttg aggggcgatc ttcctctgtt tgcggataat gattaaggcg
     5461 ccggccgtgg aacggataat aagaccgccc gttggcgaat tcttccttca atcgccggct
     5521 gatttcaatc cggaattccg gagtcacggt gatatatccc cggtcgaaaa ggcggtggaa
     5581 atcgcttcgg agaagcaacc cgttgctcac ttcatgcgga ccctcttccc catacggctt
     5641 gatatgagcg gcttccaggg cgggcaagga atgctcttcc gtaatggcgc acgaacgggc
     5701 ataagcgtcg gtcacggcca cctgaaacgc cccttgaccg agtcgaggcc ggatgacgga
     5761 ttccttgccg taacgggctc tttcctcctc cacccgccgt gtatccagat aaagatcgga
     5821 caggccttgc ctggccaagc cttcctggac gtttttccag ataaaccggc cggcgtccgt
     5881 gcccagctca taccccttgg cttttccccg gacgatcgac gctttccatt cgggcgccgg
     5941 aaaccacatc gattcctcga agaaaaacgg ctgggtcaga atgatgcatc cgattttgaa
     6001 atcctcgtga ggatcgtttt caattcttcg gtaagggagc aatctccgcc gcatgtccga
     6061 aaaggaagag gccccgtttc ggatatcgaa ggcttcccaa gccaggctga tcggcagagt
     6121 cgaaaaagat gaaaaaacgc ctcccccggc gatcacatcc ccctgccgcg ttctttgact
     6181 ccgatggagc ttgaaaagga acaaatctcc ttttccgatc gcccggaagg aggcgcttga
     6241 agacggctgc cagaagttga cttcgtcaat gccgcgaatt ccgcgtaaaa aatcaaacca
     6301 gttttgatcc gtaatcccgg cataaatcct catcgaacgt gattccctga accgtcggcg
     6361 gcaatataac aaggccgggc ggggatggca aggccccctt tctccgcgcg gcgagtaaac
     6421 gccggatggc gcccgaacgg ttacagggtg agaaaggagc tcatcatgaa aaaacccgta
     6481 ttgatcgcag ccgtggcggc cgcggttctt ttaaccgcct ctcccctcct ccggggatcg
     6541 gaaaccggcc ccgaagcgac cgtcgccaag atccgggacc ggattctggc tccggatttc
     6601 aagcctgacg gcatcgagaa ggacctgatt gaaatcctga acgtctctct cgcgatcctg
     6661 cctgaaaagg aatacgccga ggaattccgg tccaagatcg cctgggtcaa ggattcgttc
     6721 gcggacgagc atttgttcgc cgataaaatc cgccagtatc tcggaatcgc ctatgccctc
     6781 gtttccgacg ggacgagctg gcgtctcccc gaagagctcc agcgtcccta tccgggcgaa
     6841 ccggcgtcca tcaaacgggc gacggcggtc tgcggccggc ttctcgaatc ggccttggct
     6901 gaaatgaaag ccggaaacaa cgaaaaagcg gccggctgcc ttatcggctt cgtcctcctc
     6961 gtcatcaccc ccatcgaagc ctgagacggg aacggggagg cggtcggtac cggcaggatt
     7021 gatttcccgg tccgcctcaa atacattggg gaagtcaagc cggccgaaat gggaaatccc
     7081 gcgttcgatt acgattcgct gatcaaaccg ctggagcccc gaatgatgcg gacgatctgg
     7141 cggatcctcc gggagaagcc ggcggccgag gacgctctcc aggacgccct ggccgtcatc
     7201 tggaaaaagc gcgggacggt ggcccggcac cccaatcccc cggcgctcat cctccgcatc
     7261 acgattgcct cggccgtgga tagtctgcgg aagacgcggc ggcgcctccg ccacgaggtc
     7321 cccggccttc cgaaagaccg gagagcggac gattccgccg acccggccgc gggaaaggcg
     7381 gaagaacgcc ggttcagacc ggcgattctc caagccgtcg gacgcctccc cagacgccag
     7441 gcggaagcgg tcctcctgcg gatcatcgag gaactttcct acgaggagat cgcccgggcc
     7501 atgggctgca ccgaggcgac ggcccgcgtc cacgtcatgc gggggaaagc cgccctggcc
     7561 cggcggctgg cccgtttccg tccggatccg gcccgtcaac ccgggctgaa ggaataagac
     7621 aagagaggct gtcatgaggg caaaacgatt tgaagacgcg gatctggagc gcctcctgag
     7681 ggacgctctc cctgacgacc tcccggacga agccgccgcc gggatgcggg accggatcgc
     7741 cgcctttcgg accgcggcgg cgaaggaaga acggggttcg gcggtctcct tcatcctcgc
     7801 ccggaaggga gcctgggccg tcttggcgtt ggccctgttg atcgccggcg ctcttctcca
     7861 gggcttccaa tcccccagcc cgctggcaaa aaaaatcgcg ttcctcaaaa acgagctatc
     7921 gaattcggag cccgcccgtc cggccgatat cgacggggga atccggatcc ccggcctgaa
     7981 ggagggttga ccatgaacga acacgacaag aaccgggtcc gcggccgggc cgccgtccgg
     8041 tggttgatcg ccgcggcttt catcgcgggc gtcgccctct cggggctttt cgccgggatg
     8101 gcgaaccggg cgggtttatt tttcctcttc ctggggacgg ccgcctcggt cctgacgggt
     8161 ttttcccggt ccgaaatccg ggaggcgttc cggctcgcgg ccggaaaatc cggaacgccg
     8221 gaagcccggc gagaggccgc gtccttctgg gaggcggccg cccgcaacgc ctgggctctc
     8281 ggcgtcatgg gaagcctgct gaacttcacc gtcgttctcg gcggcggaag cggcgggatc
     8341 gccgacatca gcgaccggat gatccaatcg ttcattgtca ctttttacgg cctcgtcctg
     8401 gccgttctct tgctggtccc ggccatgaag atctcaggtc ttgccggaaa cggggagccc
     8461 ggcccgggtc cgaggccgtc ccgcggcggg ccctttctct tcgaacgggt gacgggctat
     8521 gtcctcttcg ccgttgttct cggactgacc gtcctgtccc tcgttcgaag cgtcccgccc
     8581 ggcggcccgc tgcccgcggc cgaagtcatg ctccatggcc cggcggccct cttcattttc
     8641 ggcggatcga tcgccctggc ccttttcctg ggaaccggaa gaggggcttg gacgttcgga
     8701 ttcggaatga cgggcctcgc cgcccttttt ctcggcttga ttcaaacgat gttcggattc
     8761 gtccacacgg acatcaagga gatctccgcg gccatcgcct tcatcatttc gtcttccctc
     8821 tatgctcttc tcggtctggt tctcgtcgcc gcccccctgg aagaccgcaa gcggccggac
     8881 gggggggatc agccggggag gctcagccga gtcctgtgga tggctttccc gttcgtggcc
     8941 ttcatcttcc tcgtcctgag ctggatcttg gtcatcacgc cgatgaaaag aaccggctga
     9001 gccgcgttcc tcctccgaaa gccacggccg tttgattgat tttccccggc cgttgatata
     9061 atcatatccc atcccccgag gaggctcccg ccatgaagcg ccgcgacttc gtctccaagg
     9121 gcctcggcac cgccgcggcc gtttcccttc ccttcctcgg aacggccgga cggccttttt
     9181 ccgccggccg cgagtccgcc ccgccccccg acggcggaac cagccgcttc aagctcggtt
     9241 acgccccgcc cttcggcctg ttcaaggccc acgccgggga cgacccggcc gcccaaatcc
     9301 gtttctgcgc cgacgagggc ttcacggcga tgttcgacaa cggcctcatg ggccggccgg
     9361 ccgcccagca ggagctgatc tcccgggagc tggccgcccg cggcatgaag ctcggcccgt
     9421 tcgtccttta ttccgatttc ggggtcaagt cgttcgtcct ccgggacaag gacgtccggg
     9481 cgatgctcgt cgaacggatg aaaaagggcg tggagacgat gaagcggacc ggctgcgccc
     9541 aagccctcgt cgttccgggg cgcttcgacg aaagcctcgc ctgggaatac cagacggcca
     9601 acgtcgtcga caacctccgg gcctgcctgg acgtctgccg cccggccggc ctcgtcctcg
     9661 tgctcgagcc cctcaatccc aaggaccacc cgggcctgtt cctgaccagg attccccaag
     9721 cctatgccct ctgccgggcg gtcgacgatc cccactgcaa gatcctcgac gacctgtatc
     9781 atcagcagat caccgagggc aacctcatcc ccaatatcga ggccgcctgg agcgagatcg
     9841 ccgctttcca cgtcggcgac agccccggcc gcaaggagcc gacgaccggg gagatcaact
     9901 accggaacat cttcaaatgg attcacggca agggatacca gggtgtcctt tgcctggagc
     9961 acggcctcag ccgccccggc aaggaaggcg agcgggcggt catcgaggcc taccgggcct
    10021 gcgacgcgtt ctaaccgtcc gcggatcgtc tcgacaaagg aggaaacgcc atgagcttga
    10081 gcaagaacat cacccgccgc gatttcatca agacgaccgc cgccgcctcc ctggccgcgg
    10141 ccattcccgg aaacctcggc ttgttcgccg ccggatcgga cgcccttcgg atcggcgtca
    10201 tcggctgcgg cggccgcgga accggagcgg cgatcaattg cctcgaggcc gccgagggaa
    10261 tcgaaatcgt cgccctcggc gacttggtcc cggaccggat cgaatcctca taccggaagc
    10321 tcatggaaaa tttcccggac cgggtccgcg tcccggccgg caaccggttc accggcttcg
    10381 acaaccatct caaggtctgc gcccttcccg aagtcaacct gatcgtcacc gccgccccgc
    10441 ccggcttccg gccgattcac ctgaaagccg cggtcgaggc cggcaagcat gtcttcatgg
    10501 agaagcccgt ggccgtcgac ccggccggcg tccgcgcggt gatcgcctcc tccgaggcgg
    10561 ccgcggctaa gggattggcc gtcgtcgccg gcacccagcg tcgccaccag aaaagctacc
    10621 tcgagctcat gcggcggatc cgggacgggc agatcggcga gatcgtcggg gcccagtgct
    10681 actggaacca aggggatctc tgggtcaagc tcccggagcc cgggatgagc gaaatggagt
    10741 ggcagtgccg caattggctc tacttcacct ggacgtccgg cgaccatatc gtcgagcagc
    10801 acgtccacaa catcgacgtg gtcaactggg cgatcggcgc cctcccgaag aacatcgtcg
    10861 ggatgggcgg ccgcgaggtc cggaaagccc ccgagtacgg caacatctac gatcatttcg
    10921 ccgtcgagtt cgagtatccg aacggcgtcc gggccgccag ctactgccgt cagaccgagg
    10981 gctgcgccga ccgggtggag gagcggatca tcgggacgaa aggcacggcc ttcggctacg
    11041 gcgagatcaa gggcgagaag ccctggacat tcgaaggtcc ggagcccgat ccctatgtcc
    11101 aggaacacgc cgacttggtc gcctcgatcc gggcgggaac gccgctgaat gagggccggc
    11161 ggatcgccga gagcacgatg tgcgcgatca tcggccggat gagcgcctac accggacggg
    11221 cgatctcctg ggattgggcg atgaacgcct cgaagctcga cctgtcgccg gcccggtacg
    11281 agttcggccc caacccggtc gatcccgtgg ccgtccccgg gaagacgccg cttatttaag
    11341 ctcggacgcg gaccggggaa ggctctaccg gatgacccgg ccgcggacaa aaccgatgga
    11401 gaccccgccg cttccggttg attccaggat cacatcgccg ccgacgtcgt cgatctcgat
    11461 atcgccggag ccgtcgtcga ccgtgacgct tccgccgatc ccggtgatcg tcatcgaccc
    11521 tgagccgtcg tcgatccgga ggccgccggt cacgtcccgg atatcgatat cgccggagcc
    11581 gtcatccacg cgcacgtcgc cctcgacgcc tctgaccgtg atgtctcccg agccgtcctc
    11641 gatcgagacg tcgccccgga tgttgacgac cgccatcgat ccggatccat cgtccacggc
    11701 caggtcgatt gtcttgggca cgcggacggt caggttgcag agcgcgtcgc cggagaaccg
    11761 gcggggaaag ccgtcgaatt ggaaatagcc tttcaggacg gctctctccc cgatcttttc
    11821 gagggaaaac cgcaggtgat tgtccatgaa atcctcgacc cgatccttgc cggctccacg
    11881 gatgatgatc tcggcgtcga cttcgatgga agacaggcct tccacgccct cgatcctcag
    11941 gcttcccgcc ccggcgttga cttcgagggc gcggatcccc tccgccggta gcgtcaggct
    12001 tttttcgacc tgataaggga aggccggcgc ggccaaaagc aaaagacaaa gcgcaaacgg
    12061 cattttcttc atgagacaaa cctcctgagc gatttcaaaa gggaagacga aaatctccat
    12121 taaaaggttc gctggaagcc cgggagggct tggcctcgga ccatttctcc cattataatc
    12181 atctctgctt ttacctgcgc ggaaaggaga gagccatgcc gaaaacgtct gattccaccc
    12241 gaaacgagcg gcaggaagcc ctcaagggca tcatcaagga tcttcacgcc ggagccacgg
    12301 tcaaggatct ccgcaagcgt ttcgccgttc tcatcaagga cacgtcggcc gaggagatcg
    12361 ccgatatgga aaacgccctc atccaggagg gtttcccggt ggaggaaatc caacgcctgt
    12421 gcgaggttca cgccgaggtc ttcgacaaat cgctccggaa agccggcaag ccatcgaaaa
    12481 tcccgggaca cccggtccac acgtatcttg aggagaaccg ggaagcccgg cgccggctca
    12541 aggacatgaa gcgggccttg aaaaagatca aggagaaccg cccgggagac cgggaaatcg
    12601 aggccttccg ggagcggttc gaggctttcc gggaaatcga aaagcattac gcccggaagg
    12661 aaaaccagct cttcccgcct ctcgaagcga cgggattcac cggcccgacc aaggtcatgt
    12721 ggggaaagca cgatgaaatc cgggcccacc tcaagtcggc cgccgacctc ttggccgccg
    12781 gcgattgggc cggcttgaaa gaccggttcc agacgctcgc ctccgcggtc aagaaaatg
//
LOCUS       DAOOXH010000069        12660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_5143_length_12660_cov_6.948671, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000069 DAOOXH010000000
VERSION     DAOOXH010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 12660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12660
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5143_length_12660_cov_6.948671"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..1245
                     /gene="murB"
                     /locus_tag="PKV64_08445"
     CDS             298..1245
                     /gene="murB"
                     /locus_tag="PKV64_08445"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPN17143.1"
                     /translation="MRLMVDIARLIEEKTGRRPSAGIRLESLSNFRIGGPADHYFEAA
                     AADELRTVVELASGSGYRHYVIGGGFNLLFDDAGYRGLIIRVACRGFIFEPKSSRLIA
                     QAGTPLSDLTGYCRDRGLGGLEFLAGIPGTVGGAVYGNAGAFGCSIGDVLEEADLLDP
                     GGKERIKARPADLSFAYRDSRLKKTREILLSAAFRAVPADKTKIGTCMDSYIALRTER
                     HPSRDTAYAGSFFKNPLSSEGTRLAAGKLLEQVGAKETTVGGAAVFHGHANFLYNRGG
                     ATAADVRKLADLLKERVMDRFGIRLEEEVIFLPAEPGAV"
     gene            complement(1211..2074)
                     /locus_tag="PKV64_08450"
     CDS             complement(1211..2074)
                     /locus_tag="PKV64_08450"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HPN17144.1"
                     /translation="MKIAVIGTGRVGTSLGNSLAQAGYEIAGLADADPAAARRARRII
                     GRGRVFRDPVAALRSAEVILIAVPDDALAGLASVLQAGEWDGRGKTLLHTSGAAPAAI
                     LAPLAKRSAAVGSMHPAQSFPTPSTPASHARGVIFGLEGDPAAVRKAKILVRSLGGRP
                     LLLTAGQKILYHAACVLASNLFVPLFGLAVETMAEAGIPAPTAKKALLPLVEGTLRNV
                     KILDAENALTGPLARLDYETVARQMRALRRVPRARDAYRTIGLAALELVKKRGIPDAR
                     VKRLRALLEGK"
     gene            complement(2071..2550)
                     /locus_tag="PKV64_08455"
     CDS             complement(2071..2550)
                     /locus_tag="PKV64_08455"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPN17145.1"
                     /translation="MAYLIDGNNLLGRLYAGYHRDPAHRAALVGKLRAFQRLTRSRVV
                     LVFDGCAPPGFVDPGKEKFAVLFPPPGESADAVIVDYIERHRDRRHLSVVSSDREIRA
                     VAREAGAADVSCDDFVRELKATLRRGREAREMEKTEERSSALDVQLWAEAFARKKHA"
     gene            complement(2552..2899)
                     /locus_tag="PKV64_08460"
     CDS             complement(2552..2899)
                     /locus_tag="PKV64_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014425321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HPN17146.1"
                     /translation="MKRDCIFCRIASKELPAKIVYEDDRIVAFDDIRPQAPVHTLIIP
                     RDHFASLNEVPAGRMDLLQHILSKIPAIAAAKEVKAGGYRVVLNTGPDSGQDVFHLHF
                     HLFGGRKMHWPPG"
     gene            complement(2939..4201)
                     /gene="murA"
                     /locus_tag="PKV64_08465"
     CDS             complement(2939..4201)
                     /gene="murA"
                     /locus_tag="PKV64_08465"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPN17147.1"
                     /translation="MEKIRIRGGPDLPLRGSVRVSGSKNAVLPAIAAALLTENKIRLE
                     NVPLVRDVYSMMTLLGELGAEGDLRENVLEMQVPRIHREEASYDLVKVMRASILVLGP
                     LVARFGKAIVALPGGCAIGSRPIDLHMTGLHKLGASITLEHGYIKAEAPRLQGADIHF
                     EKNTVGGTENIVMAASLARGETILRNCAREPEVQNLCELLVKMGAKIDGIGEETMRVT
                     GVDSLGGAVHEIIPDRIEAGTYMVAGALTRGDIILDRVRPDHLVTTIEKLRYCGARIE
                     TPETGKIRVVGSAEIHPEDITTSPYPGFPTDMQAQFMVLLTQASGTSIITETIFDRRF
                     QHVNELLRLGASIEVSGDKAIVRGPSRLSGAEVIASDLRASAGLLLAGLIAGGETVVN
                     EVEHIDRGYEAIEAKLRQLGARIERIKE"
     gene            complement(4215..5618)
                     /locus_tag="PKV64_08470"
     CDS             complement(4215..5618)
                     /locus_tag="PKV64_08470"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HPN17148.1"
                     /translation="MPDTPSYGPGVMKYVVEAQKLIAERNGLIARAKQWKFDTVAVHG
                     LYSLEESYVRNQGAVIEPLFLSDSQHYRDADELEAALAYLIPTWCYSRIANPTTYYLE
                     WTMALLESYKAGVDASCLVTASGMGAISMAVDPLLVRKNNSEKINFVSSIQVYGGTFQ
                     LFNLRKGQERGLEARWVCKPHHIEDWAAKVDENTRFLYMEAPSNPQQSFCDLRAVADL
                     AHKWGIPFIVDATCATPALMRPLAHGADIVVHSMTKSITMSGTAVGGALISRKPITTK
                     VSNDHPLFKDSYAEYVKFLPYRDNGPAAAPFNAMMALNELRTLRSRMDLVSRNCQQVA
                     EWLSGHPKIYQVDYLGLPSHPLHPLAKKYMTLVDSDDGTGREVNRYGHLMGFRVDGPP
                     ENARKVYDGFKMIYRATDLGRIKSVATIPAISTHQQQGEEARRMADVPPQLIRLCVGA
                     EDPADVIADLDQALKRL"
     gene            5789..6901
                     /locus_tag="PKV64_08475"
     CDS             5789..6901
                     /locus_tag="PKV64_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPN17149.1"
                     /translation="MSKARVAIIRTRPESVLDDIVRAFELAGGPQALKSGLPTILKDN
                     ISWHYPFPSANTTPWQLEGTILALRKHGFSDLVCVQNKTEVTDAFKGEDLNGYKPVFR
                     AHDVEVRFNFKDEDMAWVPFKPRARMRALDAIYGRSLRIPDFFIGRNIVHLPTVKCHI
                     YTTTTGAMKNAFGGLLNHKRHQTHTRIHETLVDLLAIQKEIHPGIFCLMDGTTAGNGP
                     GPRVMEPVRKDVFLAGADQVAIDAAAARLMGFDPLGIPYIRMAHEDGLGVGDPRDIEI
                     VGDEAAGAEQWGFQVGLCFHKFAGWLTWFGPTRFMQKVLTRPPVLYFANFYSFFYHDI
                     LHWKLKEGPLYRRWLEESDWGRLFTRYRREGALRPG"
     gene            6927..8480
                     /locus_tag="PKV64_08480"
     CDS             6927..8480
                     /locus_tag="PKV64_08480"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HPN17150.1"
                     /translation="MRLISKSKYLAGLQCPKLFWTYYKKPEAVPPPDAATQAIFDQGH
                     EVGALAKTLFPGGMEIEGEPWKFADLMARTREAMKKRVPLYEAAFGARHAFARADILV
                     PVESEAWDIVEVKSGTEVKPVNILDLSLQRFAAESSGLKIRKCFLMHIDNTYVRRGPV
                     EADRLFAREDVTAAVAGEMKNVEANLRSMLALLERDSSPDAPIGPHCDDPFLCPLHDA
                     CWAFLPVDNPLRVNGFSKEAAFHLIRGGILKIGDMPVIERKDAAEDSAGSPGLAASLK
                     IGKKQMIQIEAARTGLPFVKTAELRKFLEQLAYPRSYLDFETFQTAVPAYDGTRPYQQ
                     VPFQFSLHIVAAPEADPEHHSYLAAGDVDPRPEFLDRLRGCLGEFGPVICYNAAFERG
                     IVEEAVAAAPAFKAWWKKTEKRFVDLLVPFRTFAFYHPAQIGSASLKAVLPAVTGGPG
                     YEGLEICDGSRAAWEYRRITFGRVEPDERARVRKQLEEYCGLDTEAMIRVVAALEQEA
                     GRGRGESSS"
     gene            8576..9463
                     /locus_tag="PKV64_08485"
     CDS             8576..9463
                     /locus_tag="PKV64_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HPN17151.1"
                     /translation="MNRMMISLLLAGGIVFSAGAAGPAPAGGLKVFISVDMEGVSGLI
                     NWEETSASGRDYELFRRAMTEDANAAIDGALRAGATDIVVRDAHGSARNIVPDLLRPE
                     ARLLRDWSGGPMSMMEGIDDTFDAVFFIGYHARAGTPNAVLDHTNISSRLLGVVINGI
                     EMPEAGINGLMAGMFGVPVVLVSGDQAIAAQARELFGDVETAVVKEAIANAELGLHPS
                     KARALITEKAAAALKRLKDFKPYKLPPPYTLDVSFILESEAQNASWIPGAVRKDARTV
                     SFAAGTLMDLFKIFRVSKM"
     gene            complement(9572..>10369)
                     /locus_tag="PKV64_08490"
     CDS             complement(9572..>10369)
                     /locus_tag="PKV64_08490"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HPN17152.1"
                     /translation="PEIAARIYTRSYKGGKYIAPNPKLDWAANLAHQLGNDKSDFYDL
                     MRLYLDIHSDHEGGNVSAHTGHLVGSALSDAYYCLSAAMNGLAGPLHGLANQEVLRWI
                     MDVMKDLGGVPNEEQLTKYLWATLNGGKVIPGYGHAVLRKTDPRYVAQREFALKHLPD
                     YDLFKVVSSIYKVAPDILTKHGKAKNPWPNVDAHSGVLLMYYGVTEYDFYTVFFGVSR
                     ALGILSQLIWSRALGLPLERPKSVTTDWVEAQIKAASAAPGSTPSLV"
     assembly_gap    10370..10379
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10380..10920)
                     /locus_tag="PKV64_08495"
     CDS             complement(<10380..10920)
                     /locus_tag="PKV64_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HPN17153.1"
                     /translation="MATTLKEKFAGKMLPMRERIQKILKEHGDMKIADCTIAQAYGGM
                     RSVKCMVWEVSALDPNEGIRFRGLTIPDLQAKLPKLPGTEEPLPEGLFYFLLTGDIPT
                     LEDALAVGEEWKKRAKLPDYLVKVLESLPVEMHPMTQFSLAILALQKDSKFAAQYRAG
                     MNKKDYWDPMFEDVMDILAK"
     gene            11143..11697
                     /locus_tag="PKV64_08500"
     CDS             11143..11697
                     /locus_tag="PKV64_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPN17154.1"
                     /translation="MGALFVFLVILAAVVLYAIGVYNSLVTLRNRCDNSWAQVDVQLK
                     RRYDLIPNLVETVKGYAKHEREVFERVTQARNAAIAATGVKDLGAAENVLSGALKSLF
                     AVAEAYPELKANQNFLLLQEELAGTESKIAYARQFYNDVVMKFNTRQQVFPANIIAGM
                     FQFKLKEYFEIEEPAAKEPVKVQF"
     gene            11777..>12660
                     /locus_tag="PKV64_08505"
     CDS             11777..>12660
                     /locus_tag="PKV64_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPN17155.1"
                     /translation="MYEHISSNKRRSFLLIIVFIAVILLLGWVFERLTGMGEGGLILA
                     ALIAGGSALTSYYAGDKIALAVSHARPVAKEEFPYLFNVVEGLAIAAGLSAPRCYVID
                     DTAPNAFAAGRDPSHSVLCVTTGLLQKLNRVELEGVISHEMAHIKNYDVRLQTLAVVM
                     VGVVVLLGDWIMRSLWWGGRSRKISGDRSGVGAIFLIIGLFFAAFGPLVAQLLRLAVS
                     RRREFLADASGAMMTRYPAGLASALRKISADAEPLESANKATAHMYIVNPLKNLKGSL
                     NRLFSTHPPVEERIAALE"
BASE COUNT         2275 a         3818 c         4123 g         2434 t
ORIGIN      
        1 acgacggccg ttaacagcat ccttttcatg gaggacctca caaaaaattt ttttagcatc
       61 atagtcccct ttttccgtga tgtcaacaac ttggttcaac gaaacctctt cttcgtctta
      121 tatatgcaaa tcgcgtgcca aaaatccagc gaccgatccg gcggcacggc ttcgcccggc
      181 ctcgaaggac aacggctccg ggaacgaaaa cgaggacgcg aggtgcgcgg gccggacgga
      241 ctctccggcc gttgcggctt gtctctttca ttgcatcgag ctccggcccg ggatacaatg
      301 cgcctgatgg ttgatatcgc ccgattgatc gaggaaaaaa ccggccggcg cccctcggcc
      361 gggatccggc tggaaagcct gtcgaatttt cggatcgggg gcccggccga tcactatttc
      421 gaggcggccg cggccgacga gctccggacg gttgttgagc tggcctccgg ctccggatat
      481 cggcattacg tgatcggggg cggattcaat cttctgttcg acgacgccgg ctaccgcggt
      541 ttgatcatcc gggtggcatg ccgcggattc atcttcgaac cgaaatcctc ccgcttgatt
      601 gcccaagcgg ggacgcctct ttccgatctc acgggatact gccgcgaccg cggcctcgga
      661 ggcctggaat tcctggccgg catccccgga accgtggggg gggcggttta cggcaacgcg
      721 ggggcgttcg gctgctcgat cggcgatgtc ctggaggaag ccgatcttct ggatcccggc
      781 ggaaaggaac ggataaaggc ccgcccggcg gatttatcgt tcgcctaccg ggattcccgc
      841 ctcaaaaaaa cccgggaaat cctcctgtcc gcggctttcc gggcggttcc ggccgataaa
      901 acgaaaatcg ggacctgtat ggattcctat atcgccttac ggacggagcg tcatccctcg
      961 cgggatacgg cttatgccgg aagctttttc aagaatccct tgagctccga gggaacgcgg
     1021 ctggccgcgg gcaagctgct cgaacaagtc ggagccaagg agacgacggt cggcggggcg
     1081 gccgtcttcc acggccatgc gaattttctc tataatcgcg gcggggcgac ggccgccgat
     1141 gtccgcaagc tggccgacct cctcaaggag cgggtgatgg atcgtttcgg catccggctg
     1201 gaggaggagg ttattttcct tccagcagag cccggagccg tttgacccga gcgtccggaa
     1261 tcccccgttt cttcaccaat tccagggccg ccaagccgat ggtccggtac gcgtcccggg
     1321 cccgggggac ccggcgaagc gcccgcatct gccgggcgac cgtttcataa tcgagccggg
     1381 ccagggggcc ggtcagggcg ttttcggcgt caaggatttt aacgtttcgt aaagttcctt
     1441 cgaccagggg caggagggct tttttcgcgg tcggggcggg aatgccggcc tcggccatgg
     1501 tctcgaccgc gagtccgaac agcgggacga acaggttcga ggcgagaacg cacgccgcgt
     1561 ggtacaggat tttttgtccg gcggtgagaa gaaggggccg accgcccaga ctgcgaacga
     1621 ggattttcgc tttcctcacg gccgccggat cgccctcgag gccgaaaatc acgccccgcg
     1681 cgtgcgaggc cggcgtcgag ggcgtcggaa acgattgggc cggatgcatc gagccgaccg
     1741 cggcgctccg tttcgccaag ggggcgagga tggcggcggg cgcggctccg cttgtatgga
     1801 ggagcgtttt gccccgcccg tcccattccc cggcttggag gactgaggcc agcccggcca
     1861 gcgcgtcgtc cggcacggcg atgaggatga cctccgcgga gcggagggcg gcgaccggat
     1921 cccgaaacac ccggccgcgg ccgatgatcc tccgcgccct ccgggcagcc gccggatcgg
     1981 cgtcggccag gccggcgatt tcatatccgg cttgggccag ggagtttccc agcgaggtcc
     2041 ccacccgccc ggttccgatg acggcgattt tcatgcgtgt tttttccggg cgaaagcctc
     2101 ggcccaaagc tgaacgtcca gggcggacga gcgttcctcg gttttctcca tctcccgcgc
     2161 ctcccggccc cggcggagtg tggctttcag ctcccggacg aagtcatcgc agctgacgtc
     2221 cgcggctccc gcctcccggg cgacggcccg gatttcccgg tcggagctga cgaccgacaa
     2281 gtgacggcgg tcgcgatgcc gttcgatgta gtccacgatg acggcgtcgg ccgactcgcc
     2341 cggcggcggg aataagacgg cgaacttctc cttcccggga tcgacgaagc cgggcggagc
     2401 gcatccgtcg aaaacgagaa cgacccggct ccgggtcagg cgttgaaagg cgcgaagctt
     2461 gcccacgagc gccgcccggt gggcaggatc ccgatgatat ccggcgtaaa ggcggcccag
     2521 cagattgttg ccgtcgatga gataagccat gttatccggg cggccagtgc attttccggc
     2581 cgccgaagag atgaaagtgg agatggaaga cgtcttggcc ggaatccggc ccggtgttga
     2641 gcacaacccg ataaccgccg gctttgacct ccttggccgc ggcgatggcc gggatcttgg
     2701 acaggatgtg ctgaaggaga tccatccggc cggccggcac ctcgttgaga gaggcgaaat
     2761 gatcccgagg gatgatcagg gtgtgcaccg gcgcttgggg ccggatgtcg tcgaaggcga
     2821 cgatccggtc atcctcgtag acgatcttgg ccggaagctc cttggaggcg atccggcaga
     2881 aaatgcaatc tcttttcacg gcggccctcc ttggtccttg cggacgtccc cttgggagtc
     2941 actccttgat gcgctcgatc cgtgctccga gctggcggag cttggcttcg atggcttcgt
     3001 acccgcggtc gatgtgttcg acctcgttga cgacggtttc gcctccggcg atgagaccgg
     3061 ccaggagaag accggccgat gcccgaaggt ccgaagcgat cacttccgct ccggacagcc
     3121 gcgacggtcc ccggacgatc gccttgtcgc cgctgacttc gatggatgct cccagccgga
     3181 ggagctcgtt gacgtgctgg aagcggcggt cgaaaatcgt ttccgtgatg atcgaggttc
     3241 ccgaagcctg ggtcagaagg accatgaatt gggcctgcat gtccgtggga aatcccgggt
     3301 acggcgacgt cgtgatgtcc tcgggatgga tttcagcgga tccgacgacc cggattttgc
     3361 cggtttcggg agtttcgatc cgggctccgc aataacgcag cttctcgatg gtcgtgacca
     3421 ggtgatcggg gcggacgcgg tcgaggatga tgtcgccgcg cgtcaaagct ccggcgacca
     3481 tgtacgttcc ggcctcgatc cggtcgggaa tgatctcgtg gaccgctccg ccgagggagt
     3541 cgacgccggt gacccgcatc gtttcttcgc cgatgccgtc gattttcgcg cccatcttga
     3601 cgagaagctc gcagaggttc tggacctcgg gctcgcgggc gcaattgcgt agaatcgttt
     3661 cgccccgggc cagggaagcg gccatgacga tgttttccgt tccgccgacg gtgtttttct
     3721 caaagtggat gtcggcgccc tgaagacgag gggcttcggc tttgatgtac ccgtgctcca
     3781 gggtgatgga cgcccccagc ttgtgaaggc cggtcatgtg caggtcgatc ggccgggagc
     3841 cgatggcgca gccgcccggc agggcgacga tcgccttgcc gaagcgggcg accaacgggc
     3901 cgaggacgag aatggaagcc cgcatcacct tgaccagatc ataggaggct tcctcccggt
     3961 gaatcctcgg aacctgcatt tcgaggacgt tttcccgcag gtcgccttcg gccccgagct
     4021 cgccgaggag ggtcatcatg gagtagacgt cccggacaag cgggacgttc tccagccgga
     4081 ttttgttctc ggtcagcaag gcggcggcga tcgccggaag cacggcgttc ttcgacccgg
     4141 aaacgcggac gcttccccgg agagggaggt cggggcctcc tcggattcgg attttttcca
     4201 ttgtcggcgt tccttcagag ccgtttcagg gcttggtcca agtcggcgat cacgtcggcc
     4261 ggatcctcgg ctccgacgca caaccggatg agctggggag gaacgtcggc catgcgccgg
     4321 gcttcctcgc cctgctgttg gtgggtggaa atagccggga tcgtggcgac gctcttgatc
     4381 cgcccgaggt cggtggcccg gtagatcatc ttgaagccgt cgtaaacctt gcgggcgttt
     4441 tccggagggc cgtccacccg gaagcccatg agatggccgt accggttgac ttcccgcccc
     4501 gttccatcgt cggaatccac aagcgtcatg tatttcttgg ccagagggtg gagagggtgg
     4561 gagggaaggc cgaggtagtc gacctggtag attttcggat ggccggagag ccactcggcc
     4621 acttgctggc aattccggct gaccaggtcc atccgcgagc gcagggttcg taattcgttc
     4681 aaggccatca tggcgttgaa cggggcggcg gccgggccgt tgtcccgata gggaaggaac
     4741 ttcacgtact cggcgtagct gtccttgaaa agcgggtggt cgttggagac cttggtcgta
     4801 atcggcttgc ggctgatcaa agccccgccg acggccgttc cgctcatggt gatggatttc
     4861 gtcatggaat gaacgacgat gtcggccccg tgggccaggg gccgcatcag ggccggcgtg
     4921 gcgcaggtgg cgtcgacgat gaaggggatg ccccacttgt gggccaggtc ggcgaccgcc
     4981 cggaggtcgc agaacgactg ctggggattg ctcggagctt ccatgtaaag gaaccgggtg
     5041 ttttcatcca ccttggcggc ccagtcctcg atgtggtggg gcttgcagac ccagcgggct
     5101 tccaagcctc tctcctggcc cttgcgcagg ttgaagagct ggaacgtgcc tccgtagacc
     5161 tggatggagg agacgaaatt gatcttttcg ctgttatttt tccgtacgag caggggatcg
     5221 acggccatgg agatggcgcc catcccggag gccgtgacca agcacgacgc gtccaccccg
     5281 gccttgtagc tttcgaggag ggccatcgtc cattcgaggt agtaggtggt cggattggcg
     5341 atccgggagt agcaccacgt gggaatcaga taggccaggg cggcttcgag ctcgtcggcg
     5401 tcccggtaat gctgggaatc cgagaggaag agcggctcga tgaccgcgcc ttggttgcgg
     5461 acataggatt cttcgagcga gtagaggccg tgaacggcga cggtgtcgaa cttccattgc
     5521 ttggcccggg cgatcaagcc gtttcgttcg gcgatgagct tttgcgcttc gaccacgtac
     5581 ttcatgactc ccggtccgta tgagggtgtg tcgggcatgc ggtctcctcc cataagcata
     5641 ttgccggctc cggcccggcc gaagccccgt cttcttcccc tcatcatacc cgaatgccgt
     5701 tctcgaatca aaaccggtgc ggagacggcc ttggtcccgc cggggacatg gtataatccc
     5761 cttccccgga tccgggaagg agggcgatgt gagcaaagcg agggtggcga tcatccggac
     5821 ccggcccgag agcgtcctgg acgatatcgt ccgggcgttt gagctggccg gcggcccgca
     5881 ggccttgaaa agcggcctgc cgacgattct caaggacaac atcagctggc attacccctt
     5941 tccctcggcc aacacgactc cctggcagct tgaggggacg attctcgccc tccgcaagca
     6001 cggattctcc gatctcgtct gcgttcagaa caagaccgag gtcaccgacg ccttcaaggg
     6061 agaggacttg aacggctaca agcccgtctt ccgggcccat gatgtcgagg tccggttcaa
     6121 tttcaaggat gaagacatgg cctgggtgcc tttcaagccg cgggcgagga tgcgggccct
     6181 ggatgcgatc tacggccgct cgctccggat ccccgatttc ttcattgggc ggaacatcgt
     6241 ccacctgccg acggtcaagt gccacatcta cacgaccacg accggagcga tgaagaacgc
     6301 cttcggcgga cttctgaacc acaagcgcca ccagacccac acccgcattc atgagaccct
     6361 ggtcgatctc ctggcgatcc aaaaggaaat ccaccccggg attttctgcc tcatggacgg
     6421 gacgacggcc ggcaacggcc cgggcccgcg ggttatggag cctgtccgca aggacgtttt
     6481 tttggccggg gcggaccagg tcgcgatcga cgcggccgcg gcccggctga tgggcttcga
     6541 cccgctcggc attccgtaca tcaggatggc ccacgaggac ggcctcggcg tcggggatcc
     6601 gcgggacatc gagatcgtcg gggacgaggc cgcgggagcg gaacagtggg gattccaagt
     6661 cggcctctgc ttccacaagt tcgccggctg gctgacttgg ttcggaccga cccgcttcat
     6721 gcagaaggtc ctcacccggc cgccggttct ttatttcgcg aatttctatt ccttcttcta
     6781 tcatgatatc ctccactgga agctcaagga ggggcccctg taccgccgtt ggctggagga
     6841 atccgactgg ggccgcctgt tcacccgcta tcggagagaa ggggccttga ggccgggctg
     6901 agacggcggg cggcgcggga gacgcgatgc gattgatttc aaaatccaag tacctggccg
     6961 gtcttcaatg cccgaagctg ttttggacgt actataaaaa gccggaggcg gttccgccgc
     7021 ccgacgcggc cacccaggcg atcttcgacc agggacacga agtcggcgcc ctggccaaga
     7081 cgctgttccc gggggggatg gaaatcgagg gggagccgtg gaagttcgcg gacttgatgg
     7141 cccggacgcg ggaggccatg aaaaaacgag ttcctctcta tgaggcggcg tttggagccc
     7201 gacacgcctt cgccagggcg gacatcctcg ttcccgtcga aagcgaggcc tgggatatcg
     7261 tcgaggtcaa gagcggaacc gaggtcaagc cggtcaatat cctcgacctt tcgctgcagc
     7321 ggttcgcggc cgaaagcagc ggcttgaaga tccggaagtg ctttctgatg cacatcgaca
     7381 acacgtacgt ccggcggggt ccggtcgagg cggaccggct gttcgcccgg gaggacgtga
     7441 ccgcggccgt cgccggagaa atgaagaacg tcgaggcgaa cctgcgctcg atgctcgccc
     7501 tgctcgagcg ggactcctcc ccggacgcgc cgatcggccc ccattgcgac gatcccttcc
     7561 tctgtcccct ccatgacgcc tgctgggcgt tccttccggt tgacaatccg ctgagggtca
     7621 acggattcag caaggaggcc gcttttcatc tcattcgcgg gggcatcttg aaaatcggcg
     7681 acatgccggt gatcgagaga aaggatgcgg ctgaagacag cgccggatcc cccggcctcg
     7741 cggcttcctt gaagatcggg aaaaagcaaa tgatccagat cgaggcggcc cggacagggc
     7801 tcccgttcgt caaaaccgcg gagctccgaa aatttttgga acagctcgcc tatccgcgct
     7861 cctatctcga cttcgagacg tttcaaaccg ccgtgccggc gtatgacggg acgcgcccgt
     7921 atcagcaagt gccgtttcaa ttttccctcc acatcgtcgc ggcccccgag gccgatccgg
     7981 agcatcattc ctatctcgcc gccggggacg tcgacccgcg gccggaattc ctggaccggc
     8041 ttcgaggctg tttgggagag ttcggcccgg tcatctgcta taacgccgct ttcgagcgcg
     8101 gaatcgtgga agaggcggtc gcggccgcgc cggcctttaa ggcttggtgg aaaaaaacgg
     8161 agaagaggtt cgtcgatctc cttgttccgt tccggacctt cgccttctat catcccgccc
     8221 agatcggaag cgcctcgctg aaggccgttc tgccggccgt gaccggcggc cccggctatg
     8281 agggtttgga aatctgcgac ggcagccggg ccgcttggga gtatcggcgg atcacgttcg
     8341 gacgggtcga gccggacgag agagctcgag tccggaaaca gctcgaggaa tactgcggct
     8401 tggacacgga ggcgatgatc cgtgtcgtcg ccgccctgga gcaggaagcc gggaggggac
     8461 ggggggaatc ctcaagctga acgccgccgc cgtcgaggac ggcgcttctt ccgcccgctt
     8521 gccgctcgtc ctggttttca ttatcatgat cccatcattc caaggaaggt gtggcatgaa
     8581 tagaatgatg atatccctcc tgctggccgg cggaatcgtt ttttccgccg gcgccgccgg
     8641 cccggcgccc gccggcgggc tcaaggtctt catctcggtc gacatggaag gcgtcagcgg
     8701 cctgatcaac tgggaagaga cctcggcgtc cggccgggat tacgagctct tccggcgggc
     8761 gatgaccgag gacgccaacg cggccatcga cggcgcgctc cgggcggggg cgacggacat
     8821 cgtcgtccgg gacgcccacg gctcggcccg caacatcgtg ccggatctcc tgcggcccga
     8881 ggcccggctc ctccgggatt ggtcgggcgg cccgatgagc atgatggaag gcatcgacga
     8941 tacgttcgat gccgtattct ttatcgggta tcacgcccgg gccgggacgc cgaacgccgt
     9001 cctcgatcat acgaacattt cctcccggct gctgggcgtc gtcatcaacg ggatcgagat
     9061 gcccgaagcc ggaatcaacg gtctgatggc cgggatgttc ggcgttcccg ttgttctcgt
     9121 ctccggggat caggccatcg ccgcccaagc ccgggagctg ttcggcgacg tggaaaccgc
     9181 cgtcgtcaag gaagcgatcg ccaacgccga gctcggcctg cacccgtcca aagcccgggc
     9241 gctgatcacg gaaaaagcgg cggccgctct caagcggctg aaggatttca aaccgtacaa
     9301 gctcccgccg ccttataccc tggacgtctc cttcatcctg gagtcggaag cccagaacgc
     9361 gtcctggatt ccgggcgcgg tccgcaagga tgcccgcacc gtgagctttg cggccggcac
     9421 tctcatggat ctttttaaga tcttccgcgt gtccaaaatg taacggaggg tcgaaagcga
     9481 cggcgcggaa ccgcgcccaa aaaagcggag ataaaaaaag ggccgctccc cgcgcgggga
     9541 gcggcccttt cggtaaaggt cgttcaggcc gtcaaaccaa gctcggggtc gaacccggag
     9601 cggccgaggc ggccttgatc tgggcttcca cccagtcggt cgtgacggac ttcgggcgtt
     9661 caagcggcaa gccgagagcc cggctccaga tgagctggga gaggatgccg agggcccggc
     9721 tgacgccgaa gaacacggtg tagaaatcgt attccgtgac gccgtagtac atcaggagaa
     9781 cgccggagtg ggcgtcgacg ttcggccacg ggtttttggc cttgccgtgc ttcgtgagga
     9841 tgtccggggc gaccttgtaa atcgagctga cgaccttgaa caagtcgtag tcgggaaggt
     9901 gcttgagggc gaattcacgc tgggcgacat accggggatc ggtcttgcgg aggacggcgt
     9961 ggccgtaacc cgggatgacc ttcccgccgt tcagggtggc ccagaggtac ttggtcagct
    10021 gttcctcgtt gggcacgccg ccgaggtctt tcatgacgtc catgatccag cggagcactt
    10081 cctggttggc caagccgtgg agcgggccgg ccaggccgtt catggccgcc gacaggcagt
    10141 agtaggcgtc cgacagggcc gagccgacga gatggccggt atgggccgaa acgttgccgc
    10201 cctcgtgatc gctgtggatg tcgaggtaca gccgcatcag gtcgtagaaa tcggacttgt
    10261 cgttgccgag ctgatgggcc aggttggccg cccagtcgag cttcggattt ggggcgatgt
    10321 acttgccgcc cttgtaggac cgcgtgtaga tgcgggcggc gatctcgggn nnnnnnnnng
    10381 cttggcgagg atgtccatga catcctcgaa catcggatcc caataatctt ttttattcat
    10441 gccggcccgg tactgagcgg cgaatttgct gtctttctga agggccagga tggccaggga
    10501 gaactgggtc atcgggtgca tttcgacggg cagggattcg agcaccttga cgagataatc
    10561 ggggagcttg gcccgtttct tccactcctc gccgacggcc aaggcatcct cgagggtcgg
    10621 gatgtcgccg gtcaggagaa aatagaagag gccttccgga aggggctctt ccgtgccggg
    10681 cagcttgggc agcttggcct gcagatcggg aatcgtgagc ccgcggaagc ggattccctc
    10741 gttgggatcg agagccgaaa cttcccagac catgcatttg acgctgcgca tccctccgta
    10801 ggcctgggcg atcgtgcaat cggcgatctt catgtcgccg tgctccttga ggatcttttg
    10861 aatcctctct cgcatgggaa gcattttccc cgcgaatttc tccttcagag tcgtcgccat
    10921 tcgataagcc tcctttttat gaaatggctg cggtgatcat cccgaaaaca atctcgggag
    10981 aatagcgatt ttatccggaa aaataaagta agtcaacgaa aaagagcggg caggggggag
    11041 ggaaaatgcc ggaagcgctc cgtcatcccg tacttttttc tattgctttt tcgaaggggc
    11101 ggtcctattc tttagaggaa cacttcgaaa ggagaaaacg agatgggtgc tttgttcgtt
    11161 ttcctggtca tcctcgccgc ggtcgttctc tacgcgatcg gggtttacaa cagcctggtg
    11221 accctccgca accgctgcga taattcctgg gcccaggtcg atgtccagct taagcgccgg
    11281 tacgacttga tccccaacct ggtcgaaacg gtcaagggct acgccaagca cgagcgcgag
    11341 gttttcgaga gagtgaccca ggcccggaac gcggccatcg ccgccaccgg cgtcaaggac
    11401 ctgggagcgg ccgagaacgt gctgagcggc gctttgaaat ccctctttgc cgtggccgaa
    11461 gcctatcccg agctcaaagc caaccaaaat ttcctcctgc tccaggagga gctggccggg
    11521 acggaaagca agatcgctta cgcccggcag ttctacaatg acgtggtcat gaaattcaac
    11581 acgagacaac aagtctttcc ggccaacatc atcgccggga tgttccagtt caagctcaag
    11641 gaatatttcg agatcgaaga accggcggcc aaggagccgg tgaaggtcca gttctgaatt
    11701 caggataagg cctgaaacgg aggcccggcc gtcccggcgg ttgccgggac gcccccttcc
    11761 gcgccggagg cggccattgt atgaacatat ctcgagcaac aagcggcgct cgtttctcct
    11821 gattattgtt ttcattgccg tcattcttct cctgggctgg gtgttcgagc ggctgaccgg
    11881 aatgggggag gggggattga ttttagccgc cctcatcgcg gggggatcgg ccctgacgag
    11941 ctattacgcc ggggataaga tcgccttggc cgtcagccat gcccgtccgg tcgccaagga
    12001 ggagtttcct tacctgttca acgtcgtcga ggggctggcc atcgccgccg gcctgtccgc
    12061 gccccgctgc tatgtcatcg acgacacggc gcccaacgcc ttcgccgcgg gacgcgatcc
    12121 gtctcattcc gtcctctgcg tgacgacggg gttgcttcag aagctcaacc gggtcgagct
    12181 cgagggcgtc atttcccatg aaatggccca tatcaagaat tatgacgtcc gcctccagac
    12241 gctggccgtg gtgatggtcg gggtggtcgt tctcctgggc gactggatca tgcggtcgct
    12301 gtggtgggga ggacgaagcc ggaaaatcag cggcgaccgg tccggcgtcg gggccatctt
    12361 tctcatcatc gggctgttct tcgccgcttt cgggccgctt gtcgcccagc tgctccggct
    12421 ggccgtttcc cgccgccggg aattcctggc cgacgcgtcg ggagcgatga tgacccgtta
    12481 cccggccggc ctggcctcgg ccctgcggaa gatatcggcc gacgcggagc cgctcgagtc
    12541 ggccaacaag gccaccgccc atatgtacat cgtcaatccg ctgaaaaacc tcaaggggag
    12601 cctcaaccgg ctgttcagca cccatccgcc ggtcgaggag cggatcgcgg ccctggaaaa
//
LOCUS       DAOOXH010000070        12545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_5223_length_12545_cov_7.406805, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000070 DAOOXH010000000
VERSION     DAOOXH010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 12545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12545
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5223_length_12545_cov_7.406805"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..3426
                     /locus_tag="PKV64_08510"
     CDS             142..3426
                     /locus_tag="PKV64_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPN17156.1"
                     /translation="MKLARFAVERPVTVSMFFLGVILLGFVSLRELSVDLLPDIRYPR
                     LAVVTAYGGAAPEEMETLVTAALESAVSRVPGLRRVESVSKEGISHLSLEFDWGTNMD
                     FAMLHVREQLDAARDGLPEGVEDPRIVTLDPQSKPIMTLVLTGDRALLELKEIAEDLI
                     KPRLEQVEGIGSAEVTGGVEREIAVEVDPAKLALYGLTIEEIAGRIDAFNRNLQGGMI
                     RKGAFKYTLRVVGEFETIAEIADISLRVTKSGGVIRLGDVAAVRDVLKERHGITRWNG
                     EESIGLQVRKEAGANTVKATKAARKVLREIRKENPGIGIQVAAEQAGFIETAIGAVKN
                     EIIQGALLAFLILFLFLQEWKTPLIIDTVIPISVIGTFSLMYFSGLTLNLMSLGGLAL
                     GVGMLDDCAVVVSENIFRHRTLGKGLKEAAVTGTAEVGGAIVSTALTTMVVFLPVVYV
                     RGPAGQLFKDTALTVTYALSASLLVSLTLIGMLQSRRFTPAEAGKGRPWSWRWGLPPV
                     RGRNRRNAVLWPLRALRWLLYAVFKGIGFVLNGLATFAGRLLKPAVHFLGLPFRPVLR
                     AVFGGFNAAYGRFCRFNDRALVWCLDHKVRTLAATLLLFGAAFLLGSTLRRELMPPLQ
                     TKAFEIVLKTPVDYSLEQTAAMAGMIETRLGREPAVASVMSQIGIVSGLEAADPDVSM
                     NSARIHVEIDAPGRLEGVLETLRGIMVQYPDLSYRVAKDQSLLGEFLALGTAEIGLRI
                     KGNDLDRLKTIAGILTARLQAIPGLADVNTNLGEGKPEFRMSVRKDALRKYAGFSPAD
                     LGGFLVDAVNGRIATQYNEMEKKFDVRVRAEGAARRNIEALLAEPYPHERKLIPLREL
                     VDYEIVRGPREIRRENQQREVLVTAGLKNAKISEVIPNIEREIGTLDLPSGYRVVFSG
                     EREEMSRSFRSLLFALGLAVLLTYMIMAAQFESFLHPFLIMITIPMGAAGALFALWAA
                     GQTINVISVIGMVVLVGLVVDNAIVKIDYTRQLRREGTGLREAVVESSRTRLRPILMS
                     TLSTLFGLVPMALGLEAGAEMMRPLAITVIGGLTVSTFLTLILIPVLYEAVEARKERK
                     GNRGAA"
     gene            3423..6497
                     /locus_tag="PKV64_08515"
     CDS             3423..6497
                     /locus_tag="PKV64_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPN17157.1"
                     /translation="MKFLIDRPVATAMAFCALLILGVYSYLQTPLELEPKEDYPQVTI
                     SVSWPGVSPEVVQTRVTAPLEEAASAVKAVRGISSSSETGMSRVVVDFDSKADMEFVQ
                     LALREELSKLRPVLPPGLRPAVTPFVPEDFRVNPFLRYTVSGTASLQELRGLIKDRIT
                     FGLGSVRGVSRVVLTGGSDPEFRIVPDRAKLDAYGLQPYEIIRAVSGRLGIYPSGRVR
                     DGNRELLFKYGDQIRDKNDLAETIVGSAGGNVLRVRDVAAVDTTWADVDSIHRINGRP
                     TVSFTVHKEKGTNTMRVARDVKAKLALIRKDLPPDLVFQVVDDESRDIKKSLGDLYRL
                     AGLITIVVFLMIFLILRRIGPSLLILASIAFSVVITFNLIAAFGISLNMLTLGALALG
                     FGMFVDNSIVVFENILRLRENGLPPREAAVRGPREVFTAVLSSTLTTIAVFFSFPFFQ
                     GRLKIYYLPLAFVIGSALAASLLVSFTLIPALSPRLLKWRREETARRPEGIFGRFLRA
                     CLRHPVEVMLVAAVVFIGSYRWFKAEVRTGEWFSFTSRQYLFVYLGLPPGTDIERTDA
                     AIKPFEDFVLAQACAKEMNVQVSPTSATLRVSFPPAIERSAIPYRMKEELIRTASQFA
                     GLDVYVYGFDPQMYGSSMGPGSFLDSKIRFFGYNLKKLKEITAEVEKTLRLNPRIQEV
                     MITSNRHGWWGRGSSIENILKIDPERLRAYDVDPRFFYGYLQTLLQGRFGAPLVIRTE
                     GKEMAAAVKFPDADILDLRGVKDALIRTRGGEYLRLGEVADLIERPSAGSIDRDNRRF
                     QQTVSWEFRGPVKAGERFRTSLFAGLHLPAGFSASLDDDRLMTVQERSQILQAAVISL
                     LVIFMIIAALFESLTQPFIIMLAVPLGLIGVFLAFILAGATFDSSAYIGVILLAGIVV
                     NNAILLVDHINLKRKRGRPLIEAAVEGARERIRPILMTTGTTVFGILPMLLLPVESGK
                     MRIWSSLALCTAGGLVTSALFLLIVVPIVYVQVDHLRRWAAAWWSRIRSRPS"
     gene            6570..7637
                     /locus_tag="PKV64_08520"
     CDS             6570..7637
                     /locus_tag="PKV64_08520"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HPN17158.1"
                     /translation="MNPTEKTLRGKLAALPRLPLIHLPTPMRKLERLSAELGGPEIWI
                     KRDDLTGLAFGGNKSRKLEYIIPDALAKKADVLVTWGATQSNWAMQAAAAAAKSGLHP
                     VLVLFKSYDLPEEADGNILLDRLLGAEIRLQEAVRGKLVTQEIAVAAAEEVAAEFRSA
                     GKNPYLVSNGGSVPLGGMDRPLGAISYIGAFLEMLDQAESQGFRPNAVVHSTGSGGTQ
                     AGLLCGARWASPETRVVGISVSDSADSFTEIVRTVAEAVQRTLQMETGPRPEDYIVLD
                     EYLTDGYGIVNREVTGAIRRLFRTEGVVLDPVYTAKAFIGMLDLVKKGCFKKTDKIVF
                     FHTGGTPALFPNKHKLIDALD"
     gene            complement(7674..8069)
                     /locus_tag="PKV64_08525"
     CDS             complement(7674..8069)
                     /locus_tag="PKV64_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPN17159.1"
                     /translation="MKTKFVLFLAVCLVLSLASCATLFKGTTEEVNFGSNPTGAEVWV
                     DGKLMGTTPINFRLITKKVYLIEFKYQDQTKSVYLNNHVGAGWIVLDVLGGLVPVIID
                     AVTGAWYSFDQSNVNVDFRAPGAFEPAVL"
     gene            complement(8083..9024)
                     /gene="hflC"
                     /locus_tag="PKV64_08530"
     CDS             complement(8083..9024)
                     /gene="hflC"
                     /locus_tag="PKV64_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HPN17160.1"
                     /translation="MKRTTLVLYLAAALILLALLGGAVYIVSETNQVVITQFGEPVGG
                     TIIRPGLHLKIPFIQKANYFEKRWISWNGDPNQIPTRDKKYIWVDTYCRWRIADPLVF
                     FQRVDNERNARSRLDDIVDGETRNTIAKFDLIEIVRSSNRAFEISEDAAVLDASEAIS
                     KIEAGRDKLAAIILENASKITPQFGVDLKDVRIKRVNYVDEVQRKVFDRMIAERRRIA
                     AMYRSEGDGKSAEIRGQKERELKRIQSEAYKTAQEIKGKADAEATRIYAAAYNLDPEL
                     YQFMKSLDTYRAGLIKDTWLILTTDSELLKYLKNPGK"
     gene            complement(9033..9989)
                     /gene="hflK"
                     /locus_tag="PKV64_08535"
     CDS             complement(9033..9989)
                     /gene="hflK"
                     /locus_tag="PKV64_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HPN17161.1"
                     /translation="MPKLPKIPRKLIFYVLGGIILAILLAGSFYQVDPDEMGVILRFG
                     KFVRTTDPGLHFKLPLGIEALTKVPVQRQLKLEFGFRSAGGGGARTQYIAPADVEAEA
                     IMLTGDLNVVVAEWIVQYKIKDPYKFLFKMRDTEQTFRDMNEAVVRRVIGDNAVDETI
                     TTGRARIAAEAKEDLQKLCDLYEIGIDVNQLIFQDVNPPNPVKPAFNDVNEALQEKER
                     KINEAWAEYNQEIPKASGEAEQAVRAAEGYASERVNNAQGDASRFVSIYREYAKAPAV
                     TRKRLYLETMTEIMSKINRKVIADDKMKNLLPLLNLSEEVKK"
     gene            complement(10118..11323)
                     /gene="thrC"
                     /locus_tag="PKV64_08540"
     CDS             complement(10118..11323)
                     /gene="thrC"
                     /locus_tag="PKV64_08540"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPN17162.1"
                     /translation="MSLDAFICPICRRPYPADVRSLFCRACGDPLLSAFVPKSPAIRR
                     GRPMGVARYLGFLPLPEFDPDLSLGEGGTPLFPLNRLRSAFRTPPLWVKNEAVNPTGS
                     FKDRGSIVAIHMAKALGFETVGTISTGNMAGSTAAYAAKAGLRTVVFIKDDTPLEKIL
                     SAGMHGANVIRVRGNYARLFVKSFEIGRVLSFYFINSVDPYRIEGYKVTSFEIYEQMG
                     GKAPDYLFVPVSSGGHLIGLLRGFLDLKRAGRIRRLPRIVGVQARGCAPIARAFARGR
                     AAIVPVRKPQTIAHAIANPQPPAGRVVLKLLAAHGGAMEAVGETEILRAQRLLAEDEG
                     LLSDPASATVLAAFLRMSKEGRIPADAASVLVLTGSGLKTLGDVDQSRLRIAEADLSD
                     LPQLMAAWK"
     gene            11378..11878
                     /gene="tadA"
                     /locus_tag="PKV64_08545"
     CDS             11378..11878
                     /gene="tadA"
                     /locus_tag="PKV64_08545"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HPN17163.1"
                     /translation="METKISLSGQDARWMGEALREARRAAAKGEVPVGAVIVREGRAV
                     ARGGNKPIRSGDPTAHAEIVAIRAAARKTGNYRLTGCEIYVTVEPCAMCLGAILQARL
                     ARLIYGAADPKAGAVKSIIRFPIRKTNHKLDVRGGVGEEESAAVLRDFFRARRKNGPA
                     RKSEPV"
     gene            12173..12259
                     /locus_tag="PKV64_08550"
     tRNA            12173..12259
                     /locus_tag="PKV64_08550"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12207..12209,aa:Ser,seq:cga)
     assembly_gap    12268..12367
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2167 a         3842 c         3948 g         2488 t
ORIGIN      
        1 atggctgttt aattaccggc gggagctggc caacgccaag tccgctgaaa tccaggccgt
       61 catcgaatac aagatcgccc tggcccgcct cgaccggatc acgggcgcct cgccggcggc
      121 gggcggggaa cggaatccct catgaagctc gcccgcttcg ccgtcgaacg tccggtgacg
      181 gtgtcgatgt ttttcctggg cgtcattctc ttgggattcg tctccctccg ggagctgagc
      241 gtcgacctcc tgccggacat ccgctatccc cggctggccg tcgtgaccgc ttacggcgga
      301 gcggcccccg aggagatgga aaccctggtg acggccgccc tggaatcggc cgtcagccgg
      361 gtccccggcc tccgccgggt tgaatccgtc tccaaggagg ggatctccca tctcagcctc
      421 gagttcgatt ggggcacgaa catggatttc gcgatgctcc atgtccggga acagctggac
      481 gcggcccgcg acggacttcc cgagggcgtc gaggatcccc ggatcgtcac cctcgatccc
      541 cagagcaagc cgatcatgac cctggttctg accggagatc gcgcccttct cgagctcaag
      601 gagatcgccg aggacttgat caaaccccgg ctcgaacagg tcgaggggat cggttcggcc
      661 gaagtcacgg gaggcgtcga gcgggaaatc gcggtcgaag tcgaccccgc gaagctcgcc
      721 ctgtacgggc tgacgatcga ggagatcgcc ggccggatcg acgctttcaa ccgaaacctg
      781 caaggcggga tgatccggaa gggagccttt aaatatacgc tccgggtggt cggtgaattc
      841 gaaacgatcg ctgaaatcgc cgacatcagc ctccgggtca cgaaaagcgg cggggtcata
      901 cgtctcggag acgtggccgc cgtccgggac gtcctcaagg aacggcacgg gatcacccgg
      961 tggaacgggg aggagagcat cggcctccag gttcggaagg aagccggggc caacacggtc
     1021 aaagcgacga aagccgcccg gaaggtcttg cgggagatcc ggaaggaaaa ccccgggatc
     1081 ggaatccagg tcgccgccga gcaggccggc ttcatcgaaa cggcgatcgg ggcggtcaag
     1141 aatgaaatca tccagggcgc cctgctggct tttctcattc tctttttgtt ccttcaggaa
     1201 tggaaaacgc cgctcatcat cgacaccgtt atcccgatct cggttatcgg gacgttcagc
     1261 ctgatgtatt tcagcggcct caccctcaat ctgatgtcgc tcggcggcct ggccttgggg
     1321 gtgggcatgc tcgacgactg cgcggtcgtc gtttcggaaa acatcttccg ccaccggacc
     1381 ctgggcaagg gcctcaagga ggcggcggtg accggaacgg cggaggtcgg cggggcgatc
     1441 gtctcgaccg ccctgacgac aatggtcgtc tttctgcccg tcgtttacgt gcgcgggccg
     1501 gccggtcagt tgttcaagga caccgccttg accgtcacgt acgccttgag cgcgtcgctt
     1561 ctcgtttcct tgaccttgat cgggatgctc cagtcccggc ggttcacccc ggccgaggcc
     1621 gggaagggac gtccctggtc ctggcggtgg gggctgccgc ccgtccgggg aaggaatcgg
     1681 cgaaacgcgg ttctttggcc gctgcgggcc ttgcggtggt tgctgtacgc ggtcttcaag
     1741 gggatcggtt ttgtcttgaa cggcttggcg acattcgccg gccggcttct taaacctgcc
     1801 gttcattttc tcggccttcc cttccggccg gttctccggg ccgttttcgg cggattcaac
     1861 gccgcctacg gacgattttg ccggttcaac gatcgggccc tggtctggtg cctcgatcac
     1921 aaggtccgga ccttggccgc gacgctgctc ctcttcggag cggctttcct ccttggatca
     1981 acccttcgcc gggagctgat gcccccgctc cagaccaagg ctttcgagat cgtcctgaaa
     2041 acgccggtcg attattcgct cgagcagacc gcggccatgg ccggaatgat cgaaacccgg
     2101 ttgggccggg agccggccgt cgcttcggtg atgtcgcaga tcggcatcgt ttccgggctg
     2161 gaggccgccg acccggacgt ttcgatgaat tcagcccgga tccatgtcga gatagacgcg
     2221 cccgggcgtc tcgaaggggt cctcgaaact ctccgcggga tcatggtcca ataccccgac
     2281 ctcagctatc gcgtggccaa ggatcagtcg ctcctcggag aattcctggc cctggggacg
     2341 gccgagatcg gccttcggat caagggaaac gacctcgacc ggctgaaaac gatcgccgga
     2401 attttgaccg cccgccttca ggcgattccc gggctggccg acgtgaacac caacctcggc
     2461 gagggcaagc ccgagttcag gatgtcggtc cgcaaggacg ccctccgcaa gtacgccggc
     2521 ttctcgccgg ccgatctcgg cggttttttg gtcgacgcgg tcaacggccg gatcgccacc
     2581 cagtacaatg aaatggaaaa gaaattcgat gtccgcgtcc gggccgaagg ggccgcccgc
     2641 cgcaacatcg aggcgcttct ggccgagccg tatccccacg agcggaagct cattccgctc
     2701 cgcgagctcg tcgattatga gatcgtccgg ggcccgaggg aaatccggcg ggagaaccag
     2761 cagagggagg tcctagtgac cgccggcttg aaaaacgcca agatcagcga ggtcatcccg
     2821 aacatcgagc gggaaatcgg gaccctcgat ctgccttccg gataccgcgt cgtcttcagc
     2881 ggcgagcgcg aggaaatgtc ccggtccttc cggagccttc tgttcgccct cggcctggcc
     2941 gtccttttga cctatatgat catggccgcc caattcgagt cgtttctcca tcccttcctc
     3001 atcatgatca cgatcccgat gggcgcggcc ggggccttgt tcgccctgtg ggccgcgggc
     3061 cagacgatca acgtcatctc ggtcatcggg atggtcgtcc tggtcggact ggtcgtcgac
     3121 aacgccatcg tcaagatcga ttatacccgg cagcttcgcc gggaggggac gggattgcgg
     3181 gaggcggtcg tcgagtccag ccggacccgg ctgcggccca tcctgatgtc caccttgagc
     3241 acgctcttcg gcctcgtccc catggccctc ggcctggaag ccggggccga gatgatgcgg
     3301 ccgctggcga tcacggtgat cggggggttg acggtttcga cgtttttgac gctgatcctc
     3361 atccccgtcc tgtatgaagc ggtcgaggcc cggaaggaac ggaaagggaa ccggggagcc
     3421 gcgtgaaatt cctcatcgat cgtcccgtcg cgacggcgat ggctttttgc gcccttctca
     3481 tcctgggggt ctattcctat cttcaaacgc cgctcgagct ggagccgaag gaggattatc
     3541 cccaggtcac gatttcggtc tcctggcccg gcgtttcgcc cgaggtcgtg cagacgcggg
     3601 tgacggcccc cctcgaggaa gccgcctccg cggtcaaggc cgtccggggc atctcctcgt
     3661 cctcggagac cgggatgtcc cgcgtcgtcg tcgatttcga ttccaaggcc gacatggaat
     3721 tcgtccagct ggccctgcgc gaggagctct ccaagcttcg gccggtgctt ccgcccgggc
     3781 tccggccggc ggtcacgccg ttcgttcccg aggatttccg ggtgaatccc ttcctccggt
     3841 acacggtttc ggggaccgcc tccctccagg agctccgcgg gttgatcaag gataggatca
     3901 cgttcggcct cggttccgtc cggggcgtct cccgggtcgt cctgaccggg ggatccgatc
     3961 cggaattccg gatcgttccg gacagagcca agctggatgc ctacggcctt caaccgtacg
     4021 agatcatccg ggccgtctcc ggccggctgg ggatttatcc ctccggccgg gtgcgggacg
     4081 gaaaccgcga gctcctgttc aaatacggcg accaaatccg ggacaagaac gatctggcgg
     4141 agacgatcgt cggctccgcc ggcggtaacg tcctccgcgt ccgcgacgtg gccgcggtcg
     4201 acacgacctg ggcggatgtg gattccatcc accggatcaa cggccggccg accgtgagct
     4261 tcaccgtcca caaggaaaag gggacgaaca cgatgcgggt ggccagggat gtcaaggcca
     4321 agctcgccct catccggaag gacctgcctc cggacctcgt ttttcaggtc gtggacgacg
     4381 agagccggga catcaaaaaa agcctcggcg atctctaccg attagccggc ttgatcacca
     4441 tcgtcgtctt cctgatgatc ttcctcatcc tccggcggat cggaccgtcg cttctcatcc
     4501 tggcgtcgat cgccttttcc gtcgtcatca ccttcaacct catcgccgcc ttcgggattt
     4561 ccctcaacat gctgaccctc ggggcgctgg ccctgggctt cggcatgttc gtcgacaatt
     4621 ccatcgtcgt ctttgagaac atcctccgcc tgcgggaaaa cggccttccc ccgcgggagg
     4681 cggccgtccg ggggccgcgg gaggtcttca ccgccgtcct gtcatccacc ttgacgacga
     4741 tcgccgtgtt tttcagcttc ccgtttttcc aggggaggct caagatctat tacctgccgc
     4801 tggccttcgt catcggctcg gccctggccg cctccctgct ggtctccttc accctgatcc
     4861 cggccttgag cccgcgcttg ttgaaatggc gccgggagga gaccgcccgg cggccggagg
     4921 ggatcttcgg gcggttcctg cgcgcctgtc tccgccatcc ggtcgaggtg atgctcgtcg
     4981 cggccgtcgt tttcatcggt tcctaccggt ggttcaaggc cgaggtccgg acgggggagt
     5041 ggttttcctt cacctcccgg caatatctct ttgtttacct cggcctgccg ccggggacgg
     5101 acatcgagcg gacggacgcg gcgatcaagc cgttcgagga tttcgtcctg gcccaggcgt
     5161 gcgccaagga aatgaacgtc caggtcagcc ccacgagcgc cacgcttcgc gtttcttttc
     5221 cgccggcgat cgaacgctcc gcgattccgt accggatgaa agaggagctg atccggacgg
     5281 cctcccaatt cgccggcctg gacgtctatg tttacggatt cgatccccag atgtacggct
     5341 ccagcatggg accgggatcg ttcctcgact cgaaaatccg gttcttcgga tacaatctga
     5401 agaagctcaa ggaaatcacc gccgaggtgg aaaaaaccct gcggctgaat ccccggatcc
     5461 aagaagtcat gatcacctcg aacaggcacg gttggtgggg aagggggagt tcgatcgaga
     5521 acatcctgaa aatcgatccg gagcggttgc gggcctacga cgtggacccc cgcttcttct
     5581 acggatatct tcagaccttg ctccagggcc ggttcggggc gcccctggtc atccggacag
     5641 agggaaaaga aatggccgcg gccgtgaaat ttcccgacgc cgacatcctc gacctccgcg
     5701 gggtgaagga cgccttgatt cgaacaagag ggggggaata tctccgcctc ggcgaggtgg
     5761 ccgacttgat cgaacggccg tcggccggat cgatcgaccg cgataaccgg cgcttccagc
     5821 aaaccgtttc ctgggaattc cgcgggccgg tgaaggccgg cgagcgcttc cggacgagtt
     5881 tgttcgccgg tcttcatctc ccggccgggt tcagcgcctc tcttgacgac gaccggctca
     5941 tgaccgtcca agaacgatcc caaatcctcc aggcggccgt gatctcgctg ctcgtgatct
     6001 tcatgatcat cgccgccctc tttgaatccc tgacgcagcc gttcatcatc atgctggccg
     6061 tcccgctggg cttgatcggc gttttccttg cgttcatcct ggccggcgcg accttcgatt
     6121 cctcggcgta tatcggcgtc attctcctgg ccggcatcgt cgtcaacaac gccatcctgc
     6181 tcgtcgatca catcaacttg aaacggaagc gggggcgtcc cttgatcgag gcggccgtcg
     6241 agggcgctcg ggagcggatc cggccgatcc ttatgacgac cggtacgacc gtgtttggaa
     6301 tcctgccgat gctcctcctg ccggtcgaat cggggaaaat gcgaatctgg tcctccctgg
     6361 ccctctgcac cgccggcggc ctggtgacgt ccgccttgtt tctcctgatc gtcgttccca
     6421 tcgtctatgt ccaagtcgat cacctgcggc gatgggcggc ggcctggtgg agccggatcc
     6481 ggagccgtcc gtcttgaaaa gccggcccgt tgcggctaag atgaatttcc cccgccgcgg
     6541 cgggacaata tctcgtcgaa aggaccgcca tgaatcctac tgagaaaacg cttcgcggaa
     6601 agctggccgc gcttccgcgc cttcccctca ttcatcttcc cacgccgatg cggaagctcg
     6661 agcggctgtc ggccgagctg ggcgggccgg agatctggat caagagggac gacctgacgg
     6721 gcctggcctt cggtggaaat aaatcccgca agctcgagta catcatcccc gacgccctgg
     6781 ccaaaaaggc cgacgtgctg gtcacctggg gggcgaccca gtccaactgg gccatgcagg
     6841 cggccgcggc cgcggctaaa agcggccttc atccggtttt ggttctgttc aaatcctacg
     6901 atttaccgga agaagccgac gggaacattc tcctcgaccg tctcctcggc gcggagatcc
     6961 ggttgcaaga ggcggtccgg ggaaagctcg tcacccagga aatcgcggtg gcggccgccg
     7021 aggaagtcgc ggcggagttc cggtcggccg ggaaaaatcc ctatcttgtc tccaacgggg
     7081 gatcggtgcc tctgggcggc atggaccggc cgctgggggc gatctcctat ataggcgcgt
     7141 tcctcgagat gctggaccag gcggaaagcc agggcttccg gccgaatgcc gtcgtccact
     7201 caaccggctc gggcgggacg caggccgggc ttctttgcgg ggcccgctgg gcctcgcccg
     7261 aaacccgggt cgtcggcatc tcggtgtctg actcggcgga ttccttcacg gaaatcgttc
     7321 ggacggtcgc cgaggcggtc cagcggacgc ttcagatgga gacgggaccg cggccggaag
     7381 attacatcgt tttagacgaa taccttaccg acggctacgg catcgtgaac cgggaggtga
     7441 ccggggccat ccggcgcctg ttccggacgg aaggcgtcgt cctggatccc gtctatacgg
     7501 ccaaggcgtt catcgggatg ctcgacctgg tcaagaaggg ctgtttcaag aagaccgata
     7561 agatcgtctt tttccatacg ggcgggacgc cggccctttt ccccaataag cacaagctga
     7621 tcgacgccct cgactgagga ggacggaggg ggcgtcccct ccgcgtgcct cctttacagg
     7681 acggccggct cgaatgcccc cggcgcccgg aaatccacgt tgacgttgct ctggtcgaag
     7741 gaataccagg ccccggtgac cgcgtcgatg atgaccggga ccaagccgcc gaggacgtct
     7801 aaaacaatcc atcccgcgcc gacgtggttg ttcagataaa ccgacttggt ctggtcctga
     7861 tacttgaact cgatcaggta gactttcttg gtgatcaggc ggaaattgat cggcgtggtc
     7921 cccatcaatt tcccgtccac ccagacttcg gcccccgtgg gattggagcc gaaattcact
     7981 tcctcggtcg ttcccttgaa cagcgtcgcg cagcttgcca gggataaaac aaggcagacc
     8041 gcgagaaaca agacgaattt cgttttcatg ttcagcctcc gttcatttgc cgggattttt
     8101 caggtatttc aggagctcgc tgtcggtcgt caagatcagc caggtgtcct tgatcagccc
     8161 ggcccggtag gtgtcgagcg atttcatgaa ctggtagagc tcgggatcga ggttgtaggc
     8221 cgcggcgtag atgcgggtcg cctcggcgtc ggccttgccc ttgatctcct gggccgtctt
     8281 gtaggcttcc gactggatgc gcttcagctc ccgctccttc tggccgcgga tctcggcgct
     8341 cttgccgtcg ccctcggacc ggtacatcgc ggcgatcctc cggcgctcgg cgatcatccg
     8401 gtcgaagacc ttccgctgga cctcgtcgac gtaattgacc cgcttgatgc ggacgtcctt
     8461 gagatcgacg ccgaactggg gggtgatttt cgaggcgttt tccaagatga tcgccgccag
     8521 cttgtcccgg ccggcctcga tcttggagat ggcttcggac gcatcgagga cggcggcgtc
     8581 ctcggagatc tcgaaggccc ggttggacga ccggacgatc tcgatcaaat cgaacttggc
     8641 gatggtgttt cgcgtttcgc cgtcgacgat atcatccagc cgcgaccggg cgttccgctc
     8701 gttgtcgacc cgctggaaga agacgagcgg atcggcgatc cgccaccggc agtacgtgtc
     8761 gacccagatg tatttcttgt ccctggtcgg gatctggttg ggatcgccgt tccaggagat
     8821 ccagcgtttc tcgaaatagt tggccttctg gatgaagggg atcttgaggt gaaggccggg
     8881 gcggatgatc gttccgccga ccggctcgcc gaactgggtg atgacgacct ggttggtttc
     8941 gctcacgatg tagacggcgc cgccgagtag ggccaggaga atcaaggcgg cggcgagata
     9001 aagaacgagc gtggtccgtt tcatcttcgg gctcatttct tcacctcctc ggacaagttg
     9061 agaagaggca ggagattttt cattttgtcg tcggcgatca ccttgcggtt gatcttggac
     9121 atgatctcgg tcatggtctc caggtagagg cgcttgcggg tgacggccgg ggccttggcg
     9181 tattcacggt agatcgagac gaagcggctg gcgtcgccct gggcattgtt gacccgctcg
     9241 gaggcatagc cctcggcggc ccggacggct tgttcggcct cgcccgaggc cttggggatt
     9301 tcctggttgt actcggccca ggcctcgttg atcttcctct ccttctcctg gagggcttcg
     9361 ttgacgtcgt tgaaggccgg cttgaccggg ttgggcggat tgacgtcctg gaagatcagc
     9421 tggttgacgt cgatcccgat ttcatacaga tcgcagagct tctggagatc ttctttcgcc
     9481 tcggccgcga tccgggcccg gcccgtcgtg atcgtctcgt cgacggcgtt gtcgccgatg
     9541 acccggcgga cgacggcttc gttcatgtcc cggaacgttt gctcggtgtc ccgcatcttg
     9601 aacaggaact tatagggatc cttgatcttg tattggacga tccactcggc gacgacgaca
     9661 ttcaagtccc cggtcaacat gatcgcctcg gcttcgacgt cggccggggc gatgtattgg
     9721 gttcgagccc cgccgccgcc ggccgaccgg aatccgaact ccagcttcag ctgccgctgg
     9781 accgggacct tggtcagggc ttcgatgccc aagggcagct tgaagtgaag ccccggatcg
     9841 gtcgtccgga cgaacttgcc gaacctcagg atcactccca tttcatccgg gtcgacctgg
     9901 tagaacgatc cggccaggag aatcgcgagg atgattccgc cgaggacgta aaaaatcagc
     9961 ttccgaggga ttttgggaag cttgggcatt tgcccctccc atccctgaaa tggattctgg
    10021 ttggtcatgc gcctctcctc ctttcctttc gccgtcttcc atcatacgga aaacgacggc
    10081 gaaagtgaac gcggaatccc gaatcgagcc gggcgggcta tttccaggcg gccatcagct
    10141 ggggaaggtc ggacaggtcg gcctcggcga ttcggagccg gctttggtcc acgtcgccga
    10201 gggttttcag cccggatccg gtgaggacga gcaccgaagc ggcgtcggcc gggatccggc
    10261 cctccttgga cattctcaaa aaggcggcca ggaccgtggc cgaggccgga tcgctgagga
    10321 ggccctcgtc ctcggcgagc aggcgctggg cccggagaat ctcggtttcg ccgacggctt
    10381 ccatcgcccc gccgtgggcg gccaggagct tgaggacgac tcttcccgcg ggcggctgcg
    10441 ggttggcgat ggcgtgggcg atcgtttggg gcttgcggac gggaacgatg gccgcccggc
    10501 cccgggcgaa ggcccgggcg atgggggcgc agccccgggc ctggacgccg acgattcgcg
    10561 gaaggcggcg gattcggccg gcccgcttca ggtccagaaa gccgcggagg agaccgatga
    10621 ggtgcccgcc ggagctgacc ggaacgaaaa gataatcggg ggctttgccg cccatctgct
    10681 cataaatttc gaaggacgtg accttgtatc cttcgatgcg atacggatcg acggagttga
    10741 tgaagtagaa gctcaagacc cggccgatct cgaagctttt cacgaaaagg cgggcgtaat
    10801 tgccgcggac ccggatgacg ttggctccat gcatcccggc ggacaggatt ttttccaggg
    10861 gcgtgtcgtc cttgatgaag acgaccgtcc gcaagccggc cttggccgcg taggcggccg
    10921 tcgagccggc catgttgccc gttgaaatcg tcccgaccgt ctcgaagccg agggccttgg
    10981 ccatatggat ggcgacgatc gagccgcggt ctttgaagct cccggtggga ttcaccgcct
    11041 cgttcttgac ccagagcggc ggcgtccgga aagcggaccg gagacggttg agagggaaga
    11101 ggggggtccc tccttcgccc aacgagagat ccggatcgaa ttccggaaga ggaagaaagc
    11161 cgaggtagcg ggcgacgccc atcggccggc cgcggcggat cgccggcgat ttcgggacaa
    11221 aggccgagag aaggggatcg ccgcaggcgc ggcaaaacag gcttcggacg tcggccggat
    11281 agggccggcg gcagatcggg cagatgaacg catccaggct catgatcgtt gcctcttacg
    11341 gacggcgaag gaatacctta taatatttcg gcctccgatg gaaacgaaaa ttagccttag
    11401 cggacaagac gcccggtgga tgggggaagc gcttcgggaa gcgcggaggg ccgcggccaa
    11461 gggcgaggtt ccggtcgggg cggtgatcgt ccgcgagggg agggcggtgg cccggggagg
    11521 gaacaagccg atccggagcg gcgatccgac ggcccatgcc gaaatcgtcg cgatccgggc
    11581 ggcggcccgg aagacgggca attaccggtt gacggggtgt gaaatctacg tgaccgtcga
    11641 gccgtgcgcg atgtgcctcg gggcgatcct ccaggcccgg ctcgcccggc tgatctacgg
    11701 cgcggccgat cccaaggccg gggcggtgaa gtccattatc cgttttccca tccgcaaaac
    11761 gaatcacaag ctcgatgtcc ggggcggcgt gggggaggaa gagagcgccg ccgtcttgag
    11821 ggatttcttc cgcgcccgcc gcaagaacgg ccccgcccgc aaatccgaac ctgtttaacc
    11881 atttcccaat tcaaatccga acctggttat ccttttccgt atttcaatcg gtacctgttt
    11941 atccatttcc cgatttccgg atgctccgaa ttcatttaat ccgtagttga ttcactgttt
    12001 ctttcctgtc catcaatcaa tcagcaccaa gtcttccatt tcccgatttt caggaatttg
    12061 ataccggcgt ctgtttaatt gtttccgaat ttaacggcat cggattccgg acttgatctt
    12121 gatttcattt tggcgttttc gcccatatcc gctataatga gtcgcttctt gcggagaggt
    12181 gaccgagtgg ttgaaggtgc cgctctcgaa aagcggtgtg cccgtgaggg taccgcgggt
    12241 tcgaatcccg ccctctccgt tcccgcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnngga ggagcgtcta cctgcagtcc ttccctagtc atagggaagg caggatgggt
    12421 agatacggat acaaagggtc ttaaagacgc tccggtctta agaccccgca ggaggagcgt
    12481 ctacctgcag tccttcccta gtcataggga aggcaggatg ggtaggttca tataggagga
    12541 acgcc
//
LOCUS       DAOOXH010000071        12457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_5286_length_12457_cov_7.980890, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000071 DAOOXH010000000
VERSION     DAOOXH010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 12457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12457
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5286_length_12457_cov_7.980890"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /gene="proS"
                     /locus_tag="PKV64_08555"
     CDS             complement(<1..1251)
                     /gene="proS"
                     /locus_tag="PKV64_08555"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPN17164.1"
                     /translation="MTRHFSLETAVNSNERLVKQITAKSEDFSRWYAEIVQKAELADY
                     SPMKGMMIIRPYGYAVWENIQKALDLRIKATGHVNAYFPLFIPESFLQKEAEHVEGFA
                     PEVAWVTVGGKEELEERLAVRPTSEAIIGPMYSKWIKSWRDLPILYNQWANIVRWEKV
                     TRPFLRTTEFLWQEGHTCHATAEEAEEETLKILNLYRDVCETELAIPVLSGHKTLREK
                     FAGAQMTYAIEALMADGKALQMATSHNLGQHFAKAFDIKFEDRNQTLQYVWQTSWGMT
                     TRTVGALIMCHGDDSGLRFPPRVAPIQVVIVPIAAGNWKETVLPAAQAAAEALTKAGL
                     RVHLDAREEATPGWKFSEYEMRGVPLRLEIGPRDVKSGQAVCVRRDLKTKQAVPLAEL
                     TARIPALLDEIQAVLFKEAKAFLEA"
     gene            1419..1781
                     /locus_tag="PKV64_08560"
     CDS             1419..1781
                     /locus_tag="PKV64_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPN17165.1"
                     /translation="MAMKIPADEMDLLGPKQRGFTFPLPTQVEGMNAKGHDFTEETVL
                     TFISHEGSSFCLKNPIQVGSRLKMIIDLPEKLADDAPLKLVIRGRVAKVEALPGSSLT
                     QRVTIKFDSKYIIKPEDA"
     gene            1783..2121
                     /locus_tag="PKV64_08565"
     CDS             1783..2121
                     /locus_tag="PKV64_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17166.1"
                     /translation="MAEPKPGRRTFHVGLPALVQGVDLGRREFTEQTVVSAINSEEAV
                     LHLRSKVGPGAKVRLSLHVPRTFMLEKPLDLNLTGTVCEIPSRVRGARSKSSVRVRLD
                     RTFRILPASI"
     gene            2221..2910
                     /locus_tag="PKV64_08570"
     CDS             2221..2910
                     /locus_tag="PKV64_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPN17167.1"
                     /translation="MKKKTPAASGIPKTSVLLYCPSDLILAGILKSLETDETIEVVAV
                     EKGTIDSFMGAIRRLRPQVILFASAEAMPNMREICKAIRDISKDQIKTQMLLLGNIPS
                     HEEIVSLINVGARGYFDLNDPSGQLPEAVRVLAKGEIWLPRDKMSSIMDRIISVVGRE
                     IKEKTLDQLTPTEFQVLKLIGQGKSNDEIAAGLFISKNTVRSHIKSIYAKLDTHSRLQ
                     LALYAINSALF"
     gene            2933..3334
                     /locus_tag="PKV64_08575"
     CDS             2933..3334
                     /locus_tag="PKV64_08575"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPN17168.1"
                     /translation="MIKKAPAGKAGQPAVRSSAPSKIARRKEWRFPLPLAAIVSGELP
                     LGGKFREEVKIRNISSTGAFFDLEANIIVGARLRLTITLPPRATEGKKVQLKIDGLTV
                     RVEKPRKTAKKQGVAVRFTKDYHFVPAAKKA"
     gene            3345..3968
                     /gene="coaE"
                     /locus_tag="PKV64_08580"
     CDS             3345..3968
                     /gene="coaE"
                     /locus_tag="PKV64_08580"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPN17169.1"
                     /translation="MLRVALTGGIASGKSVVADVFRSRGFRFFSADQAGRKVMEPSGA
                     AYPAIVDRFGPEILAPDKSIDRSALARILFADAAARRFVEGVVHPLVLAALRAEAARL
                     AGEGRTDVFVSESALTFEAGLEGEFDRIVVVYCDPETQIQRLMDRDGLGRKEAFARIG
                     AQMDAGEKVKRADYAIDSSGPLEETIARAEAVASLLLDEARAGRLSP"
     gene            complement(3958..4899)
                     /locus_tag="PKV64_08585"
     CDS             complement(3958..4899)
                     /locus_tag="PKV64_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17170.1"
                     /translation="MASTVKPLKPGKVRTYPLASRPSKVSPADFARPPRRGERVSGLI
                     AGLPGLLSAKDLKELLELLHRARTREKTILWAMGAHVIKAGLSPVVIDLMKDGWISGL
                     ALNGAGIIHDFELAFAGRTSEDVAAQIQEGRFGMAEETGTMLNRAIREGAEAGLGLGE
                     SVGRMIARSRFPYKKLSLLGTAFRLGLPVTVHVAVGTDIIHMHPEASGEAIGACSLRD
                     FYLFCGLVEGLDGGGVYLNVGSAVILPEVFLKAVSLLRNRGRRLDGFSTAVFDFIKHY
                     RPAENVVRRPLGKKGKGFYFVGPHEILIPLLASALKG"
     gene            complement(4941..5222)
                     /locus_tag="PKV64_08590"
     CDS             complement(4941..5222)
                     /locus_tag="PKV64_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPN17171.1"
                     /translation="MIKNDIVLAIEKKTDFSAAKSLEIVEAILKALKDALADNSKVEI
                     RGFGSFRVVAKKTGYGRDIRRQKQIHITQGKRIKFRCGQELKTIAPKSE"
     gene            5385..6050
                     /locus_tag="PKV64_08595"
     CDS             5385..6050
                     /locus_tag="PKV64_08595"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPN17172.1"
                     /translation="MAKSDEYREMQISSGQLAAVILSLLALCVVIFYLGIRVGMKRAA
                     EASALKARTQTAVPAPAAAPPQITPFHEGTPTNREPSPPTAAPSAAPAKIEPAEPKPA
                     VIPAEKKTEAIPAAKTPAPAKVESKTKPPAAVKTSPASGGTYYIQAGALDTRPAAESI
                     AQRIESLGFRAIVLNPLPNDKKAVYRVRVGPYETRDAVDAALGKLAGALGKKTTDFFL
                     VKG"
     gene            6244..7354
                     /gene="prfB"
                     /locus_tag="PKV64_08600"
     CDS             join(6244..6306,6308..7354)
                     /gene="prfB"
                     /locus_tag="PKV64_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_148210098.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPN17173.1"
                     /translation="MELEAQARTAGAKFDELRGFLDLPAKKKRLEFLIAELSKPEIWQ
                     DKKKSVGLQQEKKRLEGEVCFLDGLFRSKEDLDVLFELAGEGESVIPEIETGLAAFSE
                     RLDEAELQTLFDEEDDPRNAIMTIHPGAGGTESQDWAQILFRMYLRFAERKGFKTEIL
                     DLTAGEEAGLKSATVRMEGDFVYGHLNQEIGVHRLVRISPFDSAKRRHTSFAAVFVYP
                     EIDDDIQIEISPDELKIDTYRAGGKGGQHVNTTDSAVRITHLPTGIIVQCQNERSQHK
                     NKAAAMKVLKSRLYELEQRRKREKIDKLEDAKTEIAWGNQIRSYVLHPYRMIKDNRTK
                     LETGNTDDVLDGGLDDFIKAELVYRKQQKNNKTAQ"
     gene            7545..8615
                     /locus_tag="PKV64_08605"
     CDS             7545..8615
                     /locus_tag="PKV64_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPN17174.1"
                     /translation="MRRKMIVGLTAMAFAAALAGPPSAAAGDSGGVKIGFSLSGGLGF
                     INGGDFNTYIRDWNRFVSDENRSWGEEYYTIDWKEMKSMPDFKGEIFARFGRNFGLGL
                     GVETIKKSNPGTIANEYSFASRVDYPTYYIEHAWHENLEMAVDQTLSVIPLTLGLYYF
                     LPLGSQGDVYFNAGAGVYLGTLKSNSGWSARWGSYDEFHLLDGSLYPPHIKSDSQSTQ
                     NDYYEATCNAVGFHFGAGAQFSLTDNISIFGEALYRLVNFKDWEGTGGYESTYHRDYG
                     WWTGSEWITHSIVDSEGAEEWAGGLWYSEEYDFTIGTSYSEVDLYKEKPEPTSWRDNI
                     RPAEINLNGFVFRVGIKIFFGL"
     gene            8814..9911
                     /locus_tag="PKV64_08610"
     CDS             8814..9911
                     /locus_tag="PKV64_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPN17175.1"
                     /translation="MNKRLFGGAAAVLLAILTIMSFTAVVADPPPAAAGDSGGVKIGF
                     SLSGGLGFINGGDFNTFIRDWNRYIADYNDFFRTDIMTIDWKEMKSMPDFKGEIFARF
                     GRRLGLGLGLEYIKKSNPGKIAINFASSSREDFDTYYVDFTQTYNVTDLYDQILTVIP
                     LTLNLYYHLPIGRTGDVFINAGPGYYWGTLKTDRTSTLNNDLLLELHHPDGSPWPPHF
                     KGETRIADSDQIHVTSRALGFHFGAGFDFNLSGNIALFGEGFYRLANFKEWEGTDLFR
                     SEFHQKVGWWMESAWEDYSSYDYSESEQLKGRLWHMDVRDSEMNADYCHIGFFENGPD
                     EDSPLVKNIRPAEINLNGFSFRVGIKVFFGL"
     gene            complement(10076..10864)
                     /gene="mazG"
                     /locus_tag="PKV64_08615"
     CDS             complement(10076..10864)
                     /gene="mazG"
                     /locus_tag="PKV64_08615"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPN17176.1"
                     /translation="MIEARQAGKKFQELVDILARLRGPDGCPWDRKQDIASIKNYFLE
                     EVYEAVEAIDRDDPRAAAEELGDVLMEVVFLSRLFEERGAFSAADVLAGINAKMIRRH
                     PHVFGGEKLETSDEVKNAWIVRKKAEKNRDSHFDGVPAAAPALHAAFEIGKRAADFGF
                     DWPGAAGAIDKAREELSELDQAVASGGAGKQIEELGDCLFALACAARLLNIHPETALR
                     RANAKFIARFDALESRLAENGTPLGEATLEEMEAAWQALKKERG"
     gene            complement(11092..12012)
                     /locus_tag="PKV64_08620"
     CDS             complement(11092..12012)
                     /locus_tag="PKV64_08620"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HPN17177.1"
                     /translation="MKKKALKITGFAALAGWMFASAAMGALHDETTWAQDLLRERAAK
                     DAEFKTSATSPISGRERLTIPAADKRYVVVRGGAVTVATESSPDAAFALVFREGGWFW
                     EEAAAGVEGRAEETGAALKPGIVSPGCLFRVGRLSLGAYPGPDSLALIVFDPHRPEQA
                     AFEHLLYYPPDPRYAVPARLERFPGPSQVKVTTSRLLEKTFYRYGSVHFRLDGKDLTL
                     TALKSALEGPGSDELFIPFKDATNGGETYEVGRFLDVKEPDGDALILDFNRCYNPLCN
                     YSPAYNCPMPPLENILKIGIPAGEKTYPHE"
BASE COUNT         2503 a         3921 c         3649 g         2384 t
ORIGIN      
        1 ggcctcaaga aaggccttcg cctccttgaa caacaccgcc tggatctcat ccaggagggc
       61 gggaatcctg gcggtcagct cggccagggg cacggcttgc ttggttttca agtcgcgccg
      121 gacgcagacc gcctggccgc tcttcacgtc gcgcggtccg atctcaagcc gcaggggcac
      181 gccccgcatc tcatattcgg aaaacttcca gccgggagtg gcttcctccc gggcatcgag
      241 gtgaacccgg aggccggcct tggtcagggc ttcggccgcg gcctgggcgg ccggcaggac
      301 ggtttccttc cagttgccgg cggcgatggg gacgatgacg acttgaatgg gcgcgacccg
      361 gggcgggaac cgcaggccgg aatcgtcgcc gtggcacatg atcagggcgc cgaccgtccg
      421 cgtcgtcatc ccccaggatg tctgccagac atactggagg gtttgattgc ggtcctcgaa
      481 cttgatgtcg aaggccttgg cgaaatgttg tccgagatta tgggacgtgg ccatctggag
      541 agccttgcca tcggccatga gggcctcgat ggcgtaggtc atttgggctc cggcgaactt
      601 ctcccggagg gtcttgtggc cggacaagac cgggatggcc agctcggttt cgcaaacatc
      661 ccggtacagg ttcaggatct tgagggtttc ttcctcggct tcctcggccg tggcgtgaca
      721 ggtgtggcct tcctgccaga gaaactcggt cgtccggagg aacggccggg tcactttttc
      781 ccagcggacg atgttggccc actggttgta aaggatgggc agatcccgcc aagatttaat
      841 ccacttcgaa tacatcgggc cgatgatggc ctcggacgtc ggccggacgg ccagccgctc
      901 ctccagctct tccttgccgc cgaccgtgac ccaggcgact tccggagcga atccttcgac
      961 gtgctcggct tccttctgga ggaagctctc cgggatgaac agcgggaaat aggcgttgac
     1021 gtgaccggtg gctttgatcc ggagatccag ggctttctgg atgttttccc aaacggcgta
     1081 gccgtagggt cggatgatca tcatcccttt catcggggaa taatcggcca gctcggcttt
     1141 ttggacgatt tcggcgtacc agcgggaaaa atcctcgctc ttcgcggtga tttgcttgac
     1201 aagtctttcg ttggaattca cggccgtctc cagggaaaaa tgcctggtca ttctagcccg
     1261 ccccccgcgt cctgtcaaat ggctagcatg cggccgggcg gaaggtgaga cgcaaggcgg
     1321 ggagaatcac cctttttatc accctcaggg gtgattgacc ctttccttgg gagaagccag
     1381 aataaccccg tcggaaagaa tacgaaggaa gcggaaacat ggcgatgaag attccggccg
     1441 acgagatgga tcttctgggt cccaaacagc ggggattcac cttccccctc cccacccaag
     1501 tggaggggat gaacgccaaa gggcacgatt ttaccgagga aaccgtcctg acgttcatca
     1561 gccacgaagg atcctcgttt tgcctgaaaa atcccatcca ggtcggttcc cggcttaaaa
     1621 tgatcatcga tctccccgaa aagctggccg atgacgcccc cctcaagctg gtcatccgag
     1681 gacgggtcgc caaggtcgag gctcttcccg ggtcatccct cacccagcgg gtgacgatca
     1741 agttcgacag taaatacatc atcaaacccg aggacgcctg agatggccga gccgaagccc
     1801 ggccggcgga cgttccacgt cggcttgccg gctctcgtcc aaggcgtcga ccttgggcgg
     1861 cgggaattca ccgaacagac cgtcgtttca gccatcaatt ccgaggaggc ggtcctccat
     1921 cttcgatcta aagtcggtcc gggagcgaaa gtccggctgt cccttcacgt tccccggacg
     1981 ttcatgctcg aaaaacccct cgacctcaac ctcaccggaa ccgtgtgcga gatcccgtcc
     2041 cgcgtccggg gagcgcggtc gaagtcgtcc gtccgcgtcc ggctcgaccg gacgttccgg
     2101 attcttcccg cctccatctg acatttcggc cgtccttcca agttgacttc catccttccg
     2161 ttggatagga taacagcgag ttcacataac gcgcgtcccg cgacgaaggg agcgatcggc
     2221 atgaaaaaga aaactcccgc cgcttcagga atcccgaaga cgtcggttct tctctattgc
     2281 ccctcggacc tgattctggc cgggattctg aaatcgctgg aaaccgacga aacgatcgaa
     2341 gtggtcgccg tcgagaaagg aacgatcgat tcctttatgg gagccatccg ccggctccgc
     2401 ccccaagtca tcctgttcgc cagcgccgag gcgatgccca acatgcggga gatctgcaaa
     2461 gccatccggg acatttccaa ggaccaaatc aagacccaga tgctcctgct ggggaacatc
     2521 ccctcccacg aggaaatcgt cagcctgatc aacgtcgggg cccgcggtta tttcgatctc
     2581 aacgacccgt ccgggcagtt gcccgaagcc gtccgggtcc tggccaaggg ggagatctgg
     2641 cttccccggg acaagatgtc cagcatcatg gaccggatca tctcggtcgt cggccgggaa
     2701 atcaaggaaa aaaccctcga ccagctcacc ccgacggaat tccaggttct caagctcatc
     2761 ggccagggca aatcaaacga cgagatcgcc gcagggctgt tcatcagcaa gaacacggtc
     2821 cgctcccaca tcaagagcat ctacgccaag ctcgacacgc acagccgtct ccaactcgcc
     2881 ttgtatgcca ttaattcggc tctcttctga ggcgacgaaa aggaccacgg tcatgatcaa
     2941 gaaagccccc gccggcaaag ccggccagcc ggccgtccgc tcctccgccc cgtcgaaaat
     3001 cgcccggaga aaggaatggc gcttccccct ccccctggcg gccatcgtct ccggcgaact
     3061 gcccctgggc ggaaaattcc gggaagaggt caagatcagg aatatcagct cgacgggcgc
     3121 tttcttcgac ctcgaggcca acatcatcgt cggcgcccgc ctccggctga cgatcaccct
     3181 gccgccccgg gccaccgagg gtaaaaaagt ccagctgaag atcgatggcc tcaccgtccg
     3241 ggtggaaaaa ccccgtaaaa ccgcgaaaaa acaaggcgtc gccgtccggt tcaccaagga
     3301 ttaccatttc gtcccggcgg cgaagaaggc ctgacatcgc cggcatgctc cgcgtcgcct
     3361 taaccggcgg aatcgcttcc ggcaaatccg tggtcgccga cgttttccgg agccgcggct
     3421 tccgtttctt ctcggccgat caggccggcc gcaaggtcat ggaaccctcc ggcgcggcct
     3481 acccggcgat cgtcgaccgg ttcggcccgg agatcctggc tcccgacaag tccatcgacc
     3541 gctccgccct ggcccgcatt ttattcgccg acgccgcggc ccgccggttc gtcgagggcg
     3601 tcgtccaccc cctcgtcctg gccgccctcc gggcggaagc cgcccgcctg gccggcgagg
     3661 gccggacgga cgttttcgtc tcggagtcgg ccttgacgtt cgaggccggc ctggaggggg
     3721 aattcgaccg gatcgtcgtc gtctactgcg atcccgaaac ccagatccag cggctgatgg
     3781 accgcgacgg cctcggccgc aaggaagcct tcgcccggat cggagcccaa atggacgccg
     3841 gagagaaggt caagcgggcg gattacgcga tcgactcgtc cggccccttg gaggagacga
     3901 tcgcccgggc ggaagcggtg gcttccctgc tcctggacga agcccgggcc ggccgcctca
     3961 gcccttgaga gccgaggcca gaagcgggat caggatttcg tggggcccga cgaaatagaa
     4021 tcctttcccc tttttcccga gcggccggcg gaccacgttc tcggccggcc ggtaatgctt
     4081 gatgaaatcg aagacggccg tggagaatcc gtccagccgc cggccgcggt tgcggagaag
     4141 ggacacggcc ttgagaaaaa cctcggggag gatgacggcc gaaccgacgt tgagatagac
     4201 gccgccgccg tcaagcccct cgaccagtcc gcagaacaga tagaaatccc ggagcgagca
     4261 cgctccgatc gcctcgccgc tggcctccgg atgcatgtgg atgatgtcgg ttccgacggc
     4321 cacatggacg gtgaccggca agcccaggcg gaaggccgtc ccgagcaggc tcagcttttt
     4381 ataagggaac cgcgagcggg cgatcatccg gccgacggat tctccgaggc cgagcccggc
     4441 ctccgctccc tcgcggatcg cccggttgag catcgtcccc gtttcctcgg ccatcccgaa
     4501 ccggccctcc tggatctggg cggcgacgtc ctcggacgtg cgtccggcga aagccagctc
     4561 gaaatcgtgg atgattccgg cgccgttgag ggccaagccg ctgatccagc cgtctttcat
     4621 taagtcgatg acgacaggcg agagaccggc cttgatgaca tgggcgccca tcgcccaaag
     4681 gatggttttt tcccgcgtcc gggcccgatg gagaagctcc aggagctctt tgaggtcctt
     4741 ggcggaaaga aggccgggga gtccggcgat caggccggag acgcgttccc cgcggcgggg
     4801 cggacgggcg aaatcagccg gggaaacctt gctcggccgg gaggccagcg gatacgtccg
     4861 gacttttccg ggcttcaagg gcttgacggt ggaagccacg gcgaccccct tttggcggat
     4921 tgtgtccgcg ggaatgcggc tcactcggat ttcggagcga tggtcttgag ctcctggccg
     4981 cagcggaact tgatccgctt gccctgggtg atgtggattt gcttctggcg gcggatgtcg
     5041 cggccgtatc ccgtcttctt ggccacgacc ctgaagctgc cgaagccgcg gatttcgacc
     5101 ttgctgttgt cggccagggc gtccttcaac gctttcagaa tcgcttccac gatctcgaga
     5161 gacttggccg cgctgaaatc cgttttcttt tcaatagcca gaacaatatc attcttgatc
     5221 atggaagctc ctcggaggat ctacagatta gtgattcgga gatgaaatgt caagcctatg
     5281 aaattaaaca ttttcccgtc ggaagacgga tttcgggaaa aacatttttt atccgcgcag
     5341 cgtttgactt ggccttgcgt ccttcttata atggccgtgg acaaatggca aaatcggacg
     5401 aataccggga aatgcagatc tccagcggcc agctggccgc ggtgattctt tcgctgctgg
     5461 ctctctgtgt cgtgatcttt tacctgggca tccgcgtcgg gatgaaacgg gcggccgagg
     5521 cctcggcgtt gaaagcccga acgcagacgg ccgtcccggc tcccgcggcg gcgccgcccc
     5581 aaatcacgcc gttccatgag ggaacaccga caaaccggga accttcgcct ccgaccgccg
     5641 ctccctccgc cgccccggcg aaaatcgagc cggctgaacc gaaaccggcc gtgattccgg
     5701 cggaaaagaa aaccgaggcc attcccgcgg cgaaaacgcc cgcgccggcc aaggttgaat
     5761 ccaagaccaa accgccggcc gcggtcaaaa cgagcccggc gtccggcgga acatactaca
     5821 tccaagccgg ggccctggac acccggccgg ccgccgaatc gatcgcccag cggatcgaga
     5881 gcctgggctt ccgggccatc gtcctcaatc ccctgccaaa cgacaagaag gcggtctacc
     5941 gcgtccgggt cggcccttac gaaacgaggg acgccgtgga cgcggccttg ggcaagctgg
     6001 ctggagccct gggcaagaaa acgaccgatt tcttcctcgt caaaggctga acgcccggcg
     6061 gagcccgcgc ccttgttctc cgggaagtga atcatgaaag agaaggcgct cttccccaat
     6121 tgcaaagaac ggccgcggcg ctgtttccgc ccttcttggc ttgacaagtc cggcggcctc
     6181 ccttatcctg tcgttttttg agagagaccc atgaccatca acgatacgac gaacggacgc
     6241 ctcatcgagc tcgaggccca ggcccggacg gccggggcca aatttgacga gctccgaggt
     6301 tttctttgac ctcccggcta agaaaaaacg acttgaattc ctgatcgccg agctttccaa
     6361 gcccgagatc tggcaggaca agaagaaatc cgtcggcctc cagcaggaaa aaaagcgttt
     6421 ggagggcgag gtttgcttcc tggacggtct tttccggagc aaggaggacc tggacgtcct
     6481 cttcgagctg gccggcgagg gcgagtccgt cattccggag atcgagacgg gattggccgc
     6541 cttctccgag cgcctggacg aagcggagct ccagacgctg tttgacgagg aggacgaccc
     6601 ccgcaacgcc atcatgacca tccacccggg cgccgggggg acggagtccc aggactgggc
     6661 ccagatcctt ttccgcatgt accttcgctt cgccgagcgc aaaggcttca agacggagat
     6721 cctggacctg accgccggcg aggaagcggg cctgaaaagc gccaccgtcc ggatggaggg
     6781 cgatttcgtc tacggccatc tcaaccagga gatcggcgtc caccggctgg tccgcatctc
     6841 tcccttcgac tcggccaagc ggcgccacac gtcgttcgcc gccgtcttcg tttacccgga
     6901 aatcgacgac gacatccaga tcgagatcag ccccgacgag ctcaaaatcg acacctaccg
     6961 ggccggcggc aagggcggcc agcacgtcaa cacgaccgac tcggccgtcc gcatcaccca
     7021 cctcccgacc gggatcatcg tccagtgcca gaacgagcgc tcccagcaca agaacaaggc
     7081 ggcggcgatg aaggtcctca agtcccgcct ctacgagctc gagcagcgcc gcaagcggga
     7141 aaaaatcgac aagctcgagg acgccaagac cgagatcgcc tggggcaatc agatccgctc
     7201 ctatgtcctc cacccctacc ggatgatcaa ggacaaccgg acgaagctgg agacggggaa
     7261 caccgacgac gtcctcgacg gcggcctgga cgatttcatc aaggccgagc tggtttaccg
     7321 gaaacagcag aaaaacaaca agaccgcgca ataaaaagcg gccgttccct tgactttttc
     7381 cttcttttat cttatttttt tcgtctcttg ccaaaagccg cctttcccct ctcccacaag
     7441 gcttttttga agatttttaa ctttcctccg cccgttcgga ccgcaagccc ctctccggcg
     7501 ggggaatcct tcccaaataa tcataaatta aaaaggagtt tctcatgcgt aggaaaatga
     7561 tcgtcggctt gacggccatg gcgttcgccg cggccctggc gggtcctcca tccgctgcag
     7621 ccggcgattc cggcggcgtc aagatcggct tcagcttgag cggcggcttg ggcttcatca
     7681 acggcgggga tttcaacacc tatatccggg attggaacag gttcgtttcc gacgagaacc
     7741 ggtcttgggg cgaggagtat tacacgatcg actggaagga aatgaaatcg atgcccgatt
     7801 tcaagggcga gattttcgcc cggttcggcc ggaacttcgg tctcggtctc ggcgtggaaa
     7861 cgatcaagaa atcgaatccg ggaacgatcg ccaatgaata cagctttgcg tcaagagtcg
     7921 actaccccac gtactacatc gaacatgcct ggcacgagaa cctcgagatg gccgtcgacc
     7981 agacgttgag cgtcattccc ctcaccctcg gtctttacta ttttcttccg ctcggctctc
     8041 agggcgacgt ttatttcaac gccggggcgg gagtttacct tggaacgctg aaatccaatt
     8101 ccggctggtc cgcccggtgg ggatcctacg acgagttcca tctcctggac ggaagcctgt
     8161 accctcctca tatcaagtcc gattcccaat ccactcaaaa tgattattac gaggcgacat
     8221 gcaacgccgt cggattccat ttcggggccg gcgcccagtt ttccctcacc gacaacatct
     8281 ccattttcgg cgaggcgctt taccgcctgg tcaatttcaa ggactgggag ggaacgggcg
     8341 gctatgagtc cacctaccac cgggattacg gctggtggac gggaagcgag tggatcaccc
     8401 attccatcgt cgattccgag ggcgcggaag aatgggcggg aggcctttgg tacagcgagg
     8461 aatacgactt cacgatcggc acttcatatt ccgaggtcga cctctacaag gaaaaaccgg
     8521 agccaacgtc ctggagagac aacatccggc cggcggaaat caacctcaac ggcttcgtct
     8581 tccgggtcgg aatcaagatc tttttcggac tttaagccga atataaacga gtccctcgtg
     8641 ctccttgaga caaactcgct tttcagtcca agccggccgc cggaccaagg agaaaaatac
     8701 cggcgccggc gatgaggcgg gagaggaggg atcatcgtcg atttcgagcg ccaaggaagc
     8761 gggcctgacg ggagaaaccc cgacccggca ttgatattca tcaggaggtt ctcgtgaaca
     8821 aacggctttt cggcggcgcg gccgcggtcc ttttagccat cttgacgatc atgtcgttca
     8881 cggcggttgt ggcggacccc ccgccggccg cggccggcga ttccggcggc gtcaagatcg
     8941 gcttcagcct gagcggcggc ttgggcttca tcaacggcgg ggatttcaac acctttatcc
     9001 gggattggaa cagatacatt gcggattaca atgacttttt ccgcactgat atcatgacga
     9061 tcgattggaa ggaaatgaag tcgatgccgg atttcaaggg cgagattttc gcccggttcg
     9121 gccggcgcct cggtctcggc ctcggcctgg aatacattaa aaaatcaaat ccgggaaaga
     9181 ttgcgatcaa cttcgcatcc tccagtcggg aggatttcga tacgtattat gttgatttta
     9241 cgcagacata caacgtcacc gatctctatg atcagatctt gaccgtcatt cccttgacct
     9301 tgaatctcta ttatcatctc ccgatcggcc gaacgggcga tgtcttcatc aacgccggac
     9361 cgggatacta ttggggcaca ttgaaaaccg acagaacgtc gaccctgaac aacgacttat
     9421 tgctcgagct gcatcatccc gacggatcgc cttggcctcc ccatttcaag ggcgaaacac
     9481 ggattgccga ttccgatcaa atccacgtga caagccgcgc tctcggcttt cacttcggcg
     9541 ccggcttcga cttcaacctt tccggcaata tcgccctgtt cggcgaagga ttttaccgcc
     9601 tggcgaattt caaggagtgg gaagggaccg atttgttccg atccgaattt catcagaagg
     9661 tcggttggtg gatggaaagc gcatgggaag attatagctc ttacgattac tccgagagcg
     9721 aacagctgaa gggacgactc tggcatatgg atgtccgcga ctccgaaatg aacgccgact
     9781 attgccatat cggtttcttt gaaaacgggc cggatgagga ttcccccctg gtcaagaaca
     9841 tccggccggc ggaaatcaac ctcaacggct tctccttccg cgtcggaatc aaggtctttt
     9901 tcggcttgtg aatcgagggg gggcgttccc cttttacaaa atatcgggaa tcattccgga
     9961 tttttcgtgt cttttatgac gagtcataaa atttcagccg ataatacgtc taaattcaca
    10021 aggggggggt tgatttcgta agaattcgtc ggagatcccg gcctgaccaa tagacttacc
    10081 cgcgctcctt cttgagcgcc tgccaggccg cttccatttc ctccagggtc gcctccccga
    10141 gcggcgtgcc gttttccgcc agccggcttt ccagggcgtc gaaccgggcg atgaacttgg
    10201 cgttggcccg gcgcaaggcc gtttccggat gaatgttcag aagccgggcg gcgcaggcca
    10261 gggcgaacag gcaatccccc agctcctcga tctgcttgcc ggcgccgccc gaggcgaccg
    10321 cttgatccag ctcggaaagc tcctcgcgcg ccttgtcgat ggctcccgcc gccccgggcc
    10381 agtcgaagcc gaaatcggcc gcccgcttcc cgatctcgaa agcggcatgg agggctgggg
    10441 ccgcggccgg cacgccatcg aagtgcgaat cgcgattctt ttccgctttc ttgcggacga
    10501 tccaggcgtt tttcacttca tcggaggtct cgagcttctc cccgccgaag acatggggat
    10561 ggcgccggat catcttggcg ttgatcccgg ccaggacgtc ggcggccgag aaagcgcccc
    10621 gctcctcgaa aagccgggaa agaaagacga cctccatcag gacgtcgccc agctcctcgg
    10681 ccgcggcccg gggatcatcg cggtcgatcg cctcgaccgc ctcgtagact tcctccaaga
    10741 aataattctt gatcgaagcg atgtcctgtt tccggtccca ggggcagcca tccgggccgc
    10801 gcagtcgggc caggatatcg acgagctcct gaaatttctt gccggcttga cgcgcttcaa
    10861 tcatgttctt ttcactccct ccatccgaac ctggttcacc gtttccccat tcgccggtct
    10921 cgttctcccc gcccgttgcg atcccgcgcc gcgcggcctt caagagcggg agggcggccg
    10981 gagggttccg ttttccaagc cggagccgca agggacgcgt cgccgccgga acctcgacgg
    11041 cctcggagct cccgccgtga aaaaggggaa atggaggacc aggctcggaa ttcattcatg
    11101 gggatatgtt ttttccccgg ccgggatgcc gatcttcaag atgttttcca gcgggggcat
    11161 cgggcagttg taggcgggcg agtagttgca gagcgggttg taacagcggt tgaaatccaa
    11221 aatgagcgcg tcgccgtccg gctccttcac gtcgaggaac cgcccgacct cataggtctc
    11281 cccgccgttg gtggcgtcct tgaaggggat gaacaactca tcggagcccg ggccctccaa
    11341 ggcggacttc agggcggtca gcgtcaaatc ctttccgtcc agccggaaat ggacgctgcc
    11401 gtagcggtaa aacgtctttt cgagaagccg gctggtcgtc actttcactt gggacggacc
    11461 cgggaacctc tccaagcggg ccggcaccgc gtaccgcgga tccggaggat aatagagaag
    11521 atgctcgaag gcggcctgct ccggccgatg gggatcgaag acgatcaggg ccagggaatc
    11581 ggggccggga taagcgccca acgacagccg tccgacgcgg aagaggcatc ccggggaaac
    11641 gatccccggc ttcagggcgg cgccggtctc ttccgcccgg ccttcgaccc cggccgccgc
    11701 ttcttcccag aaccacccgc cctcgcggaa aaccagggcg aaagccgcgt cgggcgagga
    11761 ttcagtcgca acggtcaccg ctccgccgcg gacgacgacg tagcgcttgt cggccgccgg
    11821 gatcgtcagg cgctcccggc cggatatggg cgacgtcgcc gaggtcttga attcggcgtc
    11881 cttggccgcc ctctcccgga gaaggtcctg ggcccacgtg gtctcgtcat gaagcgctcc
    11941 catcgccgcg gaagcgaaca tccatccggc cagggccgcg aatcccgtta tcttcaaggc
    12001 cttttttttc atcggctcct ctcttcgccg cgacaaacgg aggccgcgtc aaagccgcga
    12061 gcgggcccgg cggcgtcctg tccggtcttc ctgaccatcc cgaggagtct aatggagtgc
    12121 cgcggaagcg tcaagaagcg atctccctct ctggaatccc ccctcttcct caacgagcac
    12181 gcggggatat gccctccgcc cgacggctgg gaaaaagggc aaggggacat ggaaaccggc
    12241 ggctccgctt gaattttccg gctcccccgt ctatcatatt catcttccgt gctttgcagg
    12301 agcagaatcc catgtccgaa aaaaaagatc atgtcgccga cgcccccggc aaagggaaaa
    12361 ccttcctccc gccgctcgat ttcagctcga tcgtcgccct gctctatatc ccggccaaaa
    12421 tcaagctcgg cctcctcaac gacccggcca agaacgc
//
LOCUS       DAOOXH010000072        12449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_5293_length_12449_cov_7.613603, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000072 DAOOXH010000000
VERSION     DAOOXH010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 12449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12449
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5293_length_12449_cov_7.613603"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PKV64_08625"
     CDS             <1..666
                     /locus_tag="PKV64_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17178.1"
                     /translation="TVYFNALPGPDIAVWAVRGIGTAMMHGGTTLLFAVVTKALGERP
                     GRPRIVEALPGLGLAVLLHSAFNRFLLPPLAAAAVLITALPLLSVFVFVAGERRTRKW
                     LEAGFDSDVELLGLIGEGRFSSSRIGTYLLSLKERFPGEIVADMFCLIRIRVELAIAA
                     KGLLLMTEAGLPGRPDPGIKDKLREMAFLEKSIGPTGRRTIAPLLHGGRGDRWQRRLL
                     GGS"
     gene            complement(675..2081)
                     /locus_tag="PKV64_08630"
     CDS             complement(675..2081)
                     /locus_tag="PKV64_08630"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPN17179.1"
                     /translation="MTAMKIRFLGGIRQVTGSKFYLETPDSRVLVDCGLYQERPYLSR
                     NWQPFPVPPEDIESILLTHAHLDHCGYIPRLSADGFKGRILATPPTAELARIILLDSA
                     HIQEEDAAFKKKRHAKEGRKGPHPEIPLYTIGDAEHAILAFEPVAYEKRIVLGRRTSV
                     VFHDAGHILGSSMLQVESGEGAAVRRIVFSGDIGRWDSPLVQDPSVFSRADYVVMEST
                     YGDRDHEDPEPIESLLARIINETAGIGGKVLMPVFAMERAQEVLYYLGKLLRAKRIPP
                     LPVIMDSPMAVEITKVFERYPSYLDEEAKAIVRSGRSLFQFPGLRLTPTMADSKAVND
                     MEGPAVILAGSGMATGGRIKHHLKYNIGRPETTVLFVGYQAQGTLGRIILDGAKEIRL
                     FGAMLPVRARIVRITGFSAHAGKRELRRWLNAFDVRPRKLFIAHGDENVSLAFAAELR
                     SEGWRAVVPRYGEEFDLD"
     gene            complement(2092..2607)
                     /locus_tag="PKV64_08635"
     CDS             complement(2092..2607)
                     /locus_tag="PKV64_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17180.1"
                     /translation="MTSSVPAGDPAFFERLRRAFRGASLLGLAQIGGLAVFLIVGEII
                     RAKKRPFFGFLSSSLTSENRIILRYAFFAAAVALILLLRFLHGRRLRVIGAIDDRPAA
                     LSRLFDLTVLTLCLAEIPALLGLILFLIAGYNRDFYILLFVSLFLLFMYLPRLKNWED
                     ILEHRPPACPL"
     gene            complement(2703..3899)
                     /locus_tag="PKV64_08640"
     CDS             complement(2703..3899)
                     /locus_tag="PKV64_08640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013924960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HPN17181.1"
                     /translation="MTDLPRPDDPGGKAVLLKPGKDKAVRNRHHWIFSGAVRRFPDFE
                     DGEILDVRDANGGFLGRGYFNRRSSITGRMIAFDETPPGEAVENAVRRAAALRERLLG
                     GSTDAFRVVNGEGDGLPGLIVDRYGGVLVLQISTLGIERLKPAILDVLERTLSPETIL
                     EKSNLPSRREEGMAPFEGLLRGRPVEAVEIRENGLRFRVDLAASQKTGFYLDQRENRK
                     LVRDLAAGRRVLNAFCYTGGFTIAAWAGGAASITSLDVSAPALELARANVGLNGFSGR
                     ENTYLLGDAFETLRRLPLDFDLIILDPPAFAKRKSEVVAACRGYKDIHRIVFQKCPEG
                     ALILTFSCSYFVDEKLFRQVIFQAAGEAGRRVQIIGRHRPAGDHPVNVFHPEGDYLKG
                     WLLHAG"
     gene            complement(3896..4288)
                     /locus_tag="PKV64_08645"
     CDS             complement(3896..4288)
                     /locus_tag="PKV64_08645"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006038553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HPN17182.1"
                     /translation="MIEVTAAVIENAGRILICRRRKGGPNGGLWEFPGGKIEPGESAE
                     EALRREIKEELDLDIEVGERLGVVSGGGPSGGLVLEGFRARLRRPGGCVLRDHDDVRW
                     VVPEELAGFPFAPADRPFVELLRGRKRP"
     gene            4359..5783
                     /gene="glgA"
                     /locus_tag="PKV64_08650"
     CDS             4359..5783
                     /gene="glgA"
                     /locus_tag="PKV64_08650"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HPN17183.1"
                     /translation="MKIVFASSEAAPYAKTGGLADVASALPRALAEAGHEVFLFLPFY
                     RTVKERFPELPLRHAGRTGPFSIREARRHGVRVFFIENDYYFNRGHLYGTVEGDDPEN
                     GERFGFFSRSILDALRVLDFSPDVIHAHDWQTALIFAYRKFTNGGDPLFRSAGGVFTI
                     HNLAYQGVFEPDILGRVGLPQRLFRMDDLEFHGKVSFLKAGLLYADAVTTVSPRYARE
                     IQTPEFGCGLDGLLRSRSGAVRGIINGIDDAAWNPGADPAIAAPFSTGDLTGKKACKE
                     DLLRAFGFAPADSGPPVFGIVSRLAGQKGLDLFADALDLLAPLKFRAVIVGHGEPWIE
                     RRLAEAARRFPWNVGLRIAYDERLARKVFAGSDFFLVPSRYEPCGLTQMYAQRYGAVP
                     VVRSVGGLDDTVDEYQPASRKGTGFKFEAAGPGALVEAVRRALEAWKNPEDMAALRRN
                     GMSRDFSWARSAEEYLRLYRELAG"
     gene            complement(5976..6995)
                     /locus_tag="PKV64_08655"
     CDS             complement(5976..6995)
                     /locus_tag="PKV64_08655"
                     /inference="COORDINATES: protein motif:HMM:NF027593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HPN17184.1"
                     /translation="MTAGGGGSRKTHPPELRHDPLSGRWVLIAPDRARRPNDYRNGRP
                     AWAAGPRASCPFCPGREAETPAEVDALRGQTSVPDSPGWRIRVVPNKYPALVPGPGPS
                     PGRHEVVIDSPDHEREWADLPDGHLRDLLETFRRRLRVLENDPAVRYVQIFKNKGASA
                     GASLRHPHTQILAVPEVPRQIRDEMENLERTAAAEGEGCPVCRLIEREAAGPRLIQLN
                     PDFAALAPYASRFPYEIHVWPRRHAASFRETGEAELLILARMLKDLLGRLRAAAGDPD
                     YHLLLRQGPSAGSGAESRAGRRGFIHWRLEILPILAAVAGFEWGTGCFINPVLPESAA
                     AALRG"
     gene            complement(6992..8959)
                     /locus_tag="PKV64_08660"
     CDS             complement(6992..8959)
                     /locus_tag="PKV64_08660"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HPN17185.1"
                     /translation="MSRGFAFLFGVHNHQPVGNFGWVFDQAAADCYLPFLRALKGHPR
                     VKISAHYSGPLLEDFAARRGEAWDLLAELTARGQMELLGGGFYEPILPIIPEADRRGQ
                     IEMMSDYLEKHFGRRPRGIWLTERVWEPHLPSTLARAGIEYTLLDENHFQAAGITGLH
                     DLYVTEDEGYPLRLFPIDQRLRYLIPFRGLPELGRYLEGIRAAGGLAVLGDDGEKFGL
                     WPGTKSWVYEKGWLERFFLFLEENDIPTMTFAEAVDARPPAARIYPPPASYIEMMEWV
                     LEPDDARRYRRLKETLSEPDGRRFLRAGFFRDFFRKYPESRRLRDRMNGVSRAVRADG
                     DAEAVRDLYRGQSNDPYWHGVFGGLYLPHLREAAYGHLLKAEARCPAEPGWTKVDDDL
                     DGRPEYVRRNGRFRLQFKPGPDGGLEEIDYYPLFRNLSDVLSRRAETYHGEAGEDRGP
                     ERKGEKSIHEIAKPLPAGAADLIRPGRGPLRSAVDRFFDFEADAARIVSGEEPERGGF
                     DGEESPVVLAEDRLVCGQSGRIMVRTAASPIAVRKEIAVFGPKVLVRTILRNPGVRIL
                     RFAHAAEWNLYQIPEEIETDGDAVRLCRGRLILRPAAGAELHFRPLRTLSQSEKGYDT
                     IHQGYGLIWIRRIELEPGGTDEFTVELEESE"
     gene            complement(8956..10302)
                     /locus_tag="PKV64_08665"
     CDS             complement(8956..10302)
                     /locus_tag="PKV64_08665"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HPN17186.1"
                     /translation="MRVAILWHFHQPVYRRAGSFSYILPWVNYHVKNYHQMLSLAEET
                     GYPCTFNVVPCLIEQILDYAEGRAVDPVQAALEKDPAGLTESETALLRPFAPGECGPA
                     EVQAGALKAFLSPVDDVPKTRDACLEKQKDVLGGLVPELARLGRARRIELTASPYYHP
                     LTPLIFDLRTAFGHILPRESFHYPGDALLHLEKAVSFFEGIFGFRPAGLWPSEGAVSP
                     DVTRAAAGTGFRFAVTDENVLWKSLGVSPNLDHLRTPYRSEGLPVFFRDRLLSDLIGF
                     EYGRWDPVEAAADLVRRIKERGTSRPDDSLVLALDGENPWETYADNGVPFLREFFVRM
                     LRAEGLQPSFLGDILHDRGNDLKEIDLVPGTWLGSFAKWIGDEAKNEGWNMLSRARRD
                     CGPVEEILIAEGSDWFWWRGEDHPEFEALFRGHLASAYAGRGLPVPRECRPAEGLP"
     gene            complement(10305..11720)
                     /locus_tag="PKV64_08670"
     CDS             complement(10305..11720)
                     /locus_tag="PKV64_08670"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPN17187.1"
                     /translation="MNSCQRAAFGAAILAAALAAGGCDKKSDGNTGAGTETSPFIATA
                     VALRTEGLRSERAFTMLADLVQTVGPRLPGSPAAAAAVEHMRRVMDGLGFETWLEPTT
                     VQHWVRGEETAEMVDAAGRVVGSLTVSSLGGSVPTPESGVLAPLIEVDSFDRLDALDS
                     SARGAVVFFNRPMNPALIDTFRAYGESAVFRSGGPSRAARCGAVAALIRSVNPRIDDF
                     PHTGLVEYDPAETRIPAAAVSTADAERLSARLKENPGLRVRLKMTCADLEPVVSANVI
                     GQIRGSDKPDEIVLIGGHLDSWDLAVGAHDDGAGCVQAVEALRLIQACGLKPRRTIRA
                     VMFMNEEFGASGGRDYAADLRRKTERHVAAMESDRGGFLPLYFTIGGPPSIRRKYIAH
                     DRAFRSLGLSGIRAGGGGSDIGPIVQQGAVPMGFIPNAQTYFELHHSGLDTLDKVHPR
                     ELELGAIVLALGAYIVSEEGF"
     gene            complement(11731..12216)
                     /locus_tag="PKV64_08675"
     CDS             complement(11731..12216)
                     /locus_tag="PKV64_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HPN17188.1"
                     /translation="MGLTRYQAKRRFERTTEPSGGGGAEASVSNGGLVYVIQKHRAKR
                     LHWDLRLQEGNILKSWAVPKEPPADAGVRRLAVEVEDHPLDYALFEGTIPAGEYGAGE
                     VEIWDRGTYRVIETTPAKRWIEISGTRLRGAYVLLKLKPGEKDDRNWLFFKTKPFETQ
                     G"
     gene            complement(12260..>12449)
                     /locus_tag="PKV64_08680"
     CDS             complement(12260..>12449)
                     /locus_tag="PKV64_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17189.1"
                     /translation="DAFMPFPDVVEAAAKAGLTAIIYPLGSIRDQEVIDRADALGLAM
                     IVTRRPGEVDCERCFLHR"
BASE COUNT         2179 a         4004 c         4103 g         2163 t
ORIGIN      
        1 accgtttatt ttaatgccct tcccggaccg gacatcgccg tctgggcggt ccgggggatc
       61 gggacggcga tgatgcacgg aggaacgacg ctcctcttcg ccgtcgtcac gaaggctttg
      121 ggtgagcggc ccgggcggcc gcggatcgtc gaagcgcttc ccggtctagg cctggccgtt
      181 ctccttcatt ccgccttcaa ccggtttctc ctcccgccct tggccgcggc cgccgtcctg
      241 atcacggcct tgccgcttct ttccgttttc gttttcgtcg ccggtgaacg tcggacgcga
      301 aaatggctgg aggcgggctt cgattccgat gtcgagcttc tcggcctcat cggcgagggc
      361 cgcttctcgt cgtcccggat cgggacctat cttctttcgc tcaaggaacg gtttcccggc
      421 gagatcgtgg ccgacatgtt ctgcttgatc cggatccggg tggagctggc catcgcggcc
      481 aagggtcttc tcctgatgac ggaggcgggt cttcccggcc ggccggatcc cgggatcaag
      541 gataagctcc gggagatggc tttcctggag aaaagcatcg gcccgaccgg ccgccggacg
      601 atcgcccccc ttcttcacgg cggccggggg gaccgctggc agcgccggct tttgggcggg
      661 agctgaatcc cggatcagtc gagatcgaat tcctcgccgt agcggggaac aaccgcccgc
      721 cagccctcgg atcgaagctc cgcggcgaag gctagggaca cgttctcgtc gccgtgggcg
      781 atgaacagct tccgtggccg gacatcgaag gcgttcagcc agcgccgtag ctcgcgcttg
      841 ccggcgtggg cggaaaagcc cgtgatccgg acgatgcgcg cccggacggg gagcatcgcc
      901 ccgaagagcc ggatttcctt ggctccgtcg aggataatcc ggcccagcgt tccctgggcc
      961 tggtaaccga cgaacaagac ggtcgtttcc ggccggccga tgttgtattt caaatgatgc
     1021 ttgatccggc ctcccgtggc catccccgat ccggccagaa tgacggccgg cccctccatg
     1081 tcgttgacgg ccttggagtc ggccatggtc ggggtcagcc ggagcccggg gaactggaac
     1141 agcgagcggc cggaccggac gatcgccttg gcttcctcgt ccagataaga cgggtaccgt
     1201 tcgaagacct tcgtgatctc gacggccatc gggctgtcca tgatgaccgg aagaggcggg
     1261 atgcgcttgg cccgcaggag cttgccgagg taataaagga cctcctgggc ccgctccatg
     1321 gcgaagacgg gcatcaggac tttccccccg atgccggccg tctcgttgat aatccgggcc
     1381 agaagcgatt caatcggctc gggatcctca tgatcccggt ctccatacgt cgactccatg
     1441 acgacgtaat ctgcccggga gaagaccgag ggatcctgga cgagcggact gtcccaccgg
     1501 ccgatgtctc cggaaaagac gatccgccgg acggctgcgc cctcgcccga ctccacctgg
     1561 agcatcgagg agccgaggat gtggccggcg tcgtggaata cgaccgacgt ccgccggccc
     1621 aagacgatcc gtttttcata agccacgggc tcgaaagcca ggatcgcgtg ctccgcgtcg
     1681 cctatcgtat agagggggat ttccggatgc gggcccttgc ggccttcctt ggcgtggcgt
     1741 tttttcttga aggccgcgtc ctcctcttgg atgtgggccg aatcgaggag gatgatccgg
     1801 gccaattcgg ccgtgggagg ggtggccagg atgcggccct tgaagccgtc ggcggaaaga
     1861 cgcggaatat agccgcaatg atccagatgg gcgtgggtca ggaggatgga ctcgatgtcc
     1921 tccgggggga cgggaaacgg ctgccagttg cggctgagat aaggccgctc ctggtagagg
     1981 ccgcaatcga cgagaacgcg gctgtccggc gtctccaggt agaatttcga gcccgtgacc
     2041 tgccggatcc ctcccagaaa ccggatcttc atcgcggtca tgcgccgttt ctcagagcgg
     2101 gcaggcgggc ggccggtgct ccaggatgtc ttcccagttt ttcaaccggg ggaggtacat
     2161 gaaaaggaga aacaaggaaa cgaacaggag gatgtagaag tcccggttgt agcccgcgat
     2221 caggaagagg atcaagccca aaagagccgg gatctcggcc aggcagaggg tgaggacggt
     2281 caggtcgaac aagcgggaca aggccgcggg ccggtcgtcg atcgctccga tcaccctcag
     2341 gcgccggccg tgaagaaagc ggagaagaag gatcagggcc acggccgcgg cgaaaaacgc
     2401 gtaccgcagg atgatccggt tctcggaggt taaggaagac gacagaaagc cgaagaacgg
     2461 ccgttttttc gcccggatga tttccccgac gatcagaaaa acggccaagc ctccgatctg
     2521 ggcgaggccg agcaaggatg cgccgcggaa cgcgcggcgg agtcgttcaa aaaaagcggg
     2581 atcgcccgcc ggaacggatg aagtcatggc cgtcctttcc ttccggggaa gacggaagaa
     2641 aggctcgttc ccgccggaag accgcattgt agcacacttc ggggacggcc cgggcggttt
     2701 tctcagccgg cgtggagaag ccagcccttg agataatcgc cttccggatg aaaaacgttc
     2761 accggatgat cgcccgccgg ccggtggcgg ccgatgattt gaacgcggcg tccggcctcc
     2821 cccgcggcct ggaagatcac ctggcggaag agcttttcgt cgacgaaata ggagcaggag
     2881 aacgtcagga tgagggcgcc ctccgggcat ttttggaaaa cgatccggtg gatgtcctta
     2941 tatccgcggc aagcggcgac gacttcgctt ttgcgcttgg cgaaggccgg cggatcgaga
     3001 atgatcaggt cgaaatccag gggaagacgg cgcagcgtct cgaaggcatc gccgagcaaa
     3061 tacgtgtttt cccggccgga gaagccgttc aagccgacgt ttgcccgggc cagttccaag
     3121 gccggggcgg agacatcgag gctcgtgatg gaagccgccc cgccggccca ggcggcgatg
     3181 gtgaatcccc cggtgtagca aaaggcgttt aaaacccggc ggccggccgc caagtcccgg
     3241 acaagcttcc ggttctcccg ctgatccaag taaaagccgg ttttctgcga ggcggccagg
     3301 tcgacgcgga accggagtcc gttttcccgg atctcgaccg cctcgacggg acgtccgcga
     3361 aggagcccct cgaacggggc catgccctcc tcccgccggg acggaagatt cgatttctcg
     3421 agaatcgttt ccggagagag cgtcctttcc aggacgtcga gaatcgccgg cttgaggcgc
     3481 tcaatgccca gggtcgagat ctggaggacg aggacgcctc cgtagcggtc gacgatcaag
     3541 ccgggcaggc cgtccccctc gccgttgacc acccggaacg cgtcggtcga gccgccgagg
     3601 aggcgttcgc gcaaggcggc cgctcggcgg acggcgttct ccacggcttc gcccggagga
     3661 gtctcgtcga aagcgatcat ccgcccggtg atcgaggacc ggcggttgaa atatccccgg
     3721 ccgaggaacc cgccgttcgc gtcgcggacg tctaaaatct ctccgtcctc gaaatcggga
     3781 aagcggcgga ccgctccgga aaagatccaa tggtggcggt tgcggacggc cttgtccttg
     3841 cccggcttga gaaggacggc ttttccgccg ggatcgtccg gacgcggaag atcggtcatg
     3901 gacgtttccg gccgcggagg agttcgacga acggccggtc ggcgggagcg aaaggaaatc
     3961 ccgcgagctc ttcgggaacc acccaccgga cgtcgtcgtg atcgcggagg acgcaaccgc
     4021 ccggccgccg gagacgggcc cggaagccct ccaggacgag ccccccggag ggcccgccgc
     4081 cggagacaac gccgaggcgc tccccgacct cgatgtccag gtccagctcc tccttgatct
     4141 cccgccggag ggcctcctcc gccgattccc cgggctcgat tttccctccg gggaactccc
     4201 acaatccccc gttcggcccc cctttccgac gccggcagat gaggatgcgc ccggcgtttt
     4261 caatcaccgc cgcggtcacc tcgatcatgg ctcgaacatc gtagcgattc cggccgggtt
     4321 tggcaagacg accccggctg gggtatcatg cctccgccgt gaagattgtt ttcgcttcgt
     4381 cggaagcggc tccttatgcc aaaacggggg gcttggcgga cgtggcctcc gcccttcccc
     4441 gagccctggc cgaggcgggc cacgaggttt tcctttttct tcccttttac cggacggtca
     4501 aggaacgttt tccggagctt cccctccgcc acgccgggcg gaccggtccg ttttccatcc
     4561 gggaagcccg gcggcacggc gtccgtgttt ttttcatcga gaacgattat tactttaacc
     4621 gggggcatct ctacggcacg gtcgagggag acgatcccga aaacggagag cgcttcggct
     4681 ttttttcccg gtcgatcctc gacgctctcc gggtcctgga tttttccccg gacgtcatcc
     4741 acgcccacga ctggcagacg gccttgattt tcgcttaccg gaaattcacc aacggcggcg
     4801 atccgctgtt ccgttcggcc ggcggcgttt tcacgattca caacctggct taccaaggcg
     4861 tgttcgaacc ggacatcctc ggccgcgtcg gtcttcccca acggctgttc cggatggacg
     4921 atctcgaatt ccacggaaag gtcagctttc tgaaggccgg ccttctctac gccgacgcgg
     4981 tcacgaccgt cagcccgcgg tacgcccggg aaatccagac gccggaattc ggctgcggcc
     5041 tggacgggct tcttcgttcg cggagcggcg ccgtccgggg gatcatcaac ggcatcgacg
     5101 acgccgcctg gaatcccggc gccgacccgg cgatcgccgc ccccttctcc accggcgatc
     5161 tcacggggaa aaaagcctgc aaggaggatc tgctccgggc gttcggcttc gcccccgctg
     5221 attccggtcc gcccgtgttc gggatcgttt cccggctggc cggccagaaa gggcttgatc
     5281 ttttcgccga cgccttggac cttttggctc ccttgaagtt ccgggcggtc atcgtcggcc
     5341 acggcgagcc ctggatcgag cgccgcctgg ccgaggcggc ccggcgtttt ccctggaatg
     5401 tcggtctccg gatcgcctat gacgagcgct tggcccggaa agtcttcgcc ggcagcgatt
     5461 tttttctcgt cccgtcccgc tatgagccgt gcggactgac ccagatgtac gcccaacgat
     5521 acggggcggt cccggtcgtc cggtctgtcg gcgggctcga cgacaccgtc gatgaatatc
     5581 agcccgcgtc gcggaaggga acggggttca agttcgaggc ggccggcccc ggcgccttgg
     5641 ttgaggccgt ccgccgcgcc ttggaggcct ggaaaaatcc ggaggacatg gccgccttgc
     5701 ggcgcaacgg catgagccgg gacttttcct gggcccgctc ggccgaggag tatctccgcc
     5761 tctaccggga actggcgggc tgagacggtc cggaacaatc cggccttcaa gctcgaagcg
     5821 gccggccatc ccgtcgaccg gggccggatc cccgggatct cgccgccgcc gtctccagcg
     5881 ggcgctcgtt ccttgaaggc ggcggaaccg ccgcgtgatg aaatccaaga gccgttgacc
     5941 ggccgtgagg ctggtccggt ctattcaggc cgcgttcatc cgcggagagc cgcggccgcg
     6001 gattcgggaa gaacgggatt gatgaagcag cccgtccccc attcgaagcc ggcgacggcc
     6061 gccaggatcg ggaggatttc gaggcgccag tgaatgaacc cgcggcggcc ggcccggctt
     6121 tccgcccccg atccggccga aggcccttgg cgcaggagga gatggtaatc cggatcgccg
     6181 gccgcggccc ggagacggcc gagaaggtcc ttcagcattc gggccaggat taaaagctcg
     6241 gcctcgcccg tctcccggaa ggaggccgcg tggcgacgcg gccagacgtg gatttcatag
     6301 ggaaagcggg aggcataggg cgccagggcg gcgaaatccg gattgagttg gataagccgc
     6361 gggccggccg cctccctctc gatgagccgg cagaccgggc atccctcccc ttccgcggcc
     6421 gccgtccgct cgaggttttc catttcatcc cggatctgcc gggggacctc gggaacggcc
     6481 aggatctggg tatggggatg ccggagggac gcgccggccg aagcgccctt gttcttgaag
     6541 atctgaacgt accggacggc gggatcattc tccaagaccc ggagccgccg gcggaacgtc
     6601 tccaaaaggt ctcgaagatg gccatcgggc aggtcggccc attcccgttc gtggtccggc
     6661 gaatcgatga cgacctcgtg gcggccgggg gacgggccgg gtcccgggac aagggccgga
     6721 tacttattgg ggacgacccg gatccgccag ccgggcgaat cgggaacgga tgtttgcccg
     6781 cggagggcgt ccacttcggc gggcgtctcc gcttcgcggc cggggcagaa ggggcaggag
     6841 gcgcgcgggc cggcggccca ggccggacgg ccgtttcgat aatcgttcgg ccgccgggcc
     6901 cgatcggggg cgatcagcac ccacctcccg ctgagcgggt catggcggag ctcgggcgga
     6961 tgcgtcttcc ggctgcctcc tcctccggcg gtcattcgga ttcttccaac tcgaccgtga
     7021 attcgtccgt cccgccgggt tcgagctcga tccggcggat ccagatcaag ccgtaccctt
     7081 ggtgaatcgt gtcgtagcct ttttccgact gggacagcgt ccgaagggga cggaagtgta
     7141 gctccgcccc ggccgcgggg cgcaggatca ggcgtccccg acaaaggcgg acggcgtcgc
     7201 cgtccgtttc gatctcttcg ggaatctggt aaaggttcca ttcggcggcg tgggcgaacc
     7261 gcagaatccg aacgcccgga ttccggagga tcgtccggac gaggaccttg gggccgaaaa
     7321 cggcgatttc cttccggacg gcgatcggag aggcggccgt ccggaccatg atccgtccgg
     7381 actgcccgca gacgagccgg tcctccgcga gaacgacggg gctttcttct ccgtcgaatc
     7441 cgccccgctc cggctcctct cccgagacga tccgggccgc gtccgcctcg aaatcgaaaa
     7501 accggtccac ggccgagcgg aggggccccc ggccgggcct gatcaggtcg gccgctccgg
     7561 ccggaagggg cttggcgatt tcgtggatgc tcttttcccc tttccgctcg ggtccgcggt
     7621 cttcgccggc ctctccatga tacgtctcgg cccggcggga aagaacatcg ctcaaattcc
     7681 ggaagagagg ataataatcg atttcctcca agccgccgtc aggtccgggc ttgaattgaa
     7741 gccggaaccg gccgttgcgg cggacgtatt ccggccggcc gtcaagatcg tcatcgactt
     7801 tcgtccaacc cggctcggcc ggacaccggg cctcggcctt caacaagtgg ccgtaggccg
     7861 cttcccggag atgaggcagg tagaggccgc cgaacacgcc gtgccagtag gggtcgttgc
     7921 tctgtcctcg gtagaggtcg cggacggctt cagcgtctcc gtccgcccgg acggcccggg
     7981 agacgccgtt catccggtcg cgcagccggc ggctttcggg gtatttccga aaaaaatcac
     8041 ggaaaaaccc ggcccggaga aaacggcggc cgtcgggctc ggacagggtc tccttcaagc
     8101 ggcgataccg ccgggcgtca tccggttcca gcacccattc catcatttcg atgtaggagg
     8161 cggggggcgg ataaatccgg gcggccgggg gacgcgcgtc gaccgcctcg gcgaacgtca
     8221 tcgtcgggat gtcattttcc tcgaggaaaa gaaaaaaccg ttccaaccat cccttctcat
     8281 agacccacga tttcgtcccc ggccaaaggc cgaatttctc gccgtcgtcg cccaggacag
     8341 ccaggcctcc ggcggcccgg atgccctcca agtagcgccc gagctcgggc aggccgcgga
     8401 acggaatgag gtagcgcaat ctctggtcga tcggaaacag ccggagcgga tatccctcgt
     8461 cctcggtcac gtaaaggtcg tggaggccgg tgatcccggc ggcttggaaa tgattttcat
     8521 ccaggagcgt atactcgatg ccggctcggg ccagggtgga gggcaagtgg ggctcccaga
     8581 cgcgctcggt cagccagatc ccccggggcc gccggccgaa atgcttctcc aggtagtcgc
     8641 tcatcatctc gatctgacct ctcctgtcgg cctcggggat gatcggaaga atcggctcgt
     8701 aaaatccgcc gccgagaagc tccatctgtc cccgggcggt cagctcggcc aggaggtccc
     8761 aggcttcgcc gcgccgggcg gcgaagtcct ccagcagcgg gccggaataa tgggccgaga
     8821 ttttcacccg cgggtggcct ttcagggctc gcaggaacgg caggtagcaa tcggccgcgg
     8881 cctggtcgaa gacccagccg aaattgccga cgggctggtg attgtggaca ccgaataaaa
     8941 aagcgaatcc gcggctcatg gaagtccttc cgcgggccgg cattccctgg ggaccggaag
     9001 gccccggccg gcgtaggcgg aggcgagatg cccgcgaaag agggcctcga attcaggatg
     9061 gtcctctccc cgccaccaga accagtccga tccctcggcg atcaggattt cctccaccgg
     9121 gccgcaatcc cgccgggctc ggctgagcat gttccatccc tcgtttttag cttcgtcgcc
     9181 gatccacttg gcgaagcttc cgagccaggt tcccgggacg agatcgattt ccttaagatc
     9241 gttcccgcgg tcgtggagaa tatctccgag aaaagacggc tggaggccct cggcccggag
     9301 catccggacg aaaaactccc ggagaaaggg aacgccgttg tcggcgtagg tttcccaagg
     9361 attctctccg tccaaggcca gaaccaggga gtcgtccggg cgggatgtgc ctcgctcctt
     9421 gattcttcga accaggtcgg ccgccgcctc gacagggtcc catcggccgt actcgaagcc
     9481 gatcaagtcg gacagcagcc ggtcccgaaa aaaaacgggc aagccctcgc tccggtacgg
     9541 cgtccggaga tggtccaagt tcggagagac gccgaggctt ttccaaagga cgttttcgtc
     9601 cgtcacggcg aagcgaaaac ccgtcccggc cgcggcccgc gtcacgtcgg ggctgaccgc
     9661 gccctcggac ggccagaggc cggccggccg gaagccgaaa atcccctcga aaaaggaaac
     9721 ggccttttcc agatgaagga gagcgtcgcc gggataatgg aacgattccc gcggcaggat
     9781 atggccgaaa gccgtcctca ggtcgaagat caacggcgtc aagggatggt agtacgggga
     9841 agccgtcagc tcgatccgcc gcgcccgtcc gagccgggcc agttccggga caagcccgcc
     9901 gaggacgtct ttttgcttct ccaagcatgc gtccctcgtt ttgggtacat catcgaccgg
     9961 cgaaagaaag gccttcaggg ctcctgcctg gacttcggcc gggccgcatt cgcccggggc
    10021 gaacgggcgg agaagagcgg tctcgctttc ggtcaagccc gccgggtcct tttccagggc
    10081 ggcctggacg ggatcgacgg cccggccttc ggcgtaatcg aggatttgtt caatcaggca
    10141 gggaaccacg ttgaaggtgc agggatatcc cgtctcctcc gccagggaaa gcatctggtg
    10201 gtaatttttc acgtggtagt tgacccaggg aaggatgtag gagaacgacc cggcccggcg
    10261 gtagaccggc tgatggaaat gccagaggat ggcgacccgc atcctcagaa tccttcttcc
    10321 gagacgatat aggctccaag ggcgagaacg atcgccccaa gctcaagctc gcggggatgg
    10381 accttgtcca gggtgtcgag gccggaatga tgcagctcaa aataggtctg ggcgttggga
    10441 atgaagccca tgggcacggc gccttgttgg acgatcggtc cgatgtccga tcctcccccg
    10501 ccggcccgga tgccggacaa gccgagactc cggaacgccc ggtcgtgggc gatgtattta
    10561 cgccggatcg agggcgggcc gccgatcgtg aaataaaggg gaagaaatcc gccccggtcc
    10621 gattccatgg ccgcgacatg acgctcggtt ttccgccgga gatcggccgc gtaatcccgg
    10681 ccgcccgagg cgccgaattc ctcgttcatg aacatcaccg cccggatcgt ccgccgcggt
    10741 ttcaggccgc aggcttggat caggcgcaga gcctcgacgg cttggacgca tcccgcgccg
    10801 tcgtcgtgcg ctccgacggc caaatcccag gaatccagat ggccgccgat caggacgatc
    10861 tcgtccggct tatcggatcc gcggatctga ccgatcacgt tggccgagac gacgggttcc
    10921 aggtcggcgc acgtcatttt cagacggacc cgcaagccgg gattctcctt gagccgggcg
    10981 gaaagccgtt cggcgtccgc ggtcgagacg gcggccgccg gaatcctcgt ctcggccgga
    11041 tcgtactcaa ccaggccggt atggggaaaa tcatcgatcc ggggattcac cgagcggatc
    11101 aaggccgcga ccgccccgca acgggcggcc cgggacggtc cgccggaacg gaaaacggct
    11161 gattctccgt aagcccggaa cgtatcgatg agtgccgggt tcatcggccg gttgaaaaaa
    11221 acgaccgctc cccgcgccga tgaatccaag gcgtcgagcc ggtcgaacga gtcgacctcg
    11281 atcaaaggag ccaggacgcc ggattcgggc gtcggaacgc ttcctcccag ggaagagacg
    11341 gtcagcgaac caacgacgcg cccggcggcg tcgaccatct cggccgtctc ctccccccgg
    11401 acccagtgct ggaccgtcgt cggttcgagc cacgtttcaa agccgaggcc gtccatgacc
    11461 cggcgcatgt gctcgaccgc ggcggccgcg gccggtgagc ccgggaggcg cggcccgacg
    11521 gtctggacaa gatcggcgag cattgtgaac gcgcgttcgc ttcgaagccc ctcggtccgg
    11581 agagcgacgg ccgtggcgat aaagggggac gtctccgttc ccgcgccggt attgccatcg
    11641 gattttttat cgcatccgcc ggcggcgagg gccgcggcca ggatcgcggc cccgaaggcc
    11701 gcccgttgac aagaattcat tttttcgtcc tcaaccttgc gtttcgaacg gttttgtctt
    11761 gaagaagagc cagttgcggt catccttttc cccgggcttg agcttcagca gcacatacgc
    11821 cccccggagg cgcgttccgg agatctcgat ccagcgcttc gccggggtcg tttcgatgac
    11881 ccggtaagtc ccgcggtccc agatttcgac ctcccccgcg ccgtattctc cggccgggat
    11941 ggttccctcg aacaaggcgt agtccaaggg atgatcctcg acttcaacgg ccaggcgcct
    12001 cacgccggcg tccgccggag gctccttggg gacggcccag cttttcaaaa tgtttccttc
    12061 ctgaaggcgg agatcccagt ggaggcgctt ggcccggtgt ttttgaatga catagacgag
    12121 tccgccgttg gagacggagg cttccgcccc gccgcctccc gacggctcgg tcgtccgctc
    12181 gaaccgtctc ttggcttggt atcgcgtcaa tcccatggct tgtttcccgg gacggcgccc
    12241 tcctcgccgc cggcggcggt cagcgatgga ggaagcagcg ctcgcaatcg acttctccgg
    12301 ggcgccgggt gacgatcatc gccaggccca gggcgtcggc ccggtcgatc acttcttgat
    12361 cccggatcga gcccagcgga tagatgatcg cggtcaatcc cgccttggcc gcggcctcga
    12421 cgacgtcggg aaagggcatg aaagcgtcc
//
LOCUS       DAOOXH010000073        12396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_5318_length_12396_cov_8.647597, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000073 DAOOXH010000000
VERSION     DAOOXH010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 12396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12396
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5318_length_12396_cov_8.647597"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PKV64_08685"
     CDS             complement(<1..618)
                     /locus_tag="PKV64_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17190.1"
                     /translation="MAFTGHETFEPAPTATIIHAYNSDGVSLVTLTDEEIYAVIKASG
                     ANLIGRPNMHPRVTIMDRYIAERGIEVSQVEQSLAIEESHASYFWEKQKIGKSSYESA
                     CMEVNTCGDSLSPKVLGTFLDACDYPELVSFVYFAPTPLLKDYKISDRESLIKQCFTN
                     PPECYSRYNVDCLPAIDIPAGKLFVAHLYRRDVSGLIEELREGVVK"
     gene            complement(631..2451)
                     /locus_tag="PKV64_08690"
     CDS             complement(631..2451)
                     /locus_tag="PKV64_08690"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPN17191.1"
                     /translation="MNRIDALNGIAKRLRAQSMRMTTASASGHPTTCLSAAEIITALF
                     FGGMRFNPADPNDWGNDELVLSKGHAAPILWAAYAEAGILPAEALLDFRKITSGLEGH
                     PTPRMPWIKVATGSLGQGLSVGAGMAAAMRLGKSPARVFVLCGDGECAEGSVWEAANS
                     GADMKLRNLCAIVDVNRLGQSGPTQHQRDAAALARKFRAFGWDAVVIDGHKIEAVLAA
                     LKAAGSKGLPTAVLAKTVKGKGVSFLEDLNGWHGKPLKADELEKALAELGPIPEIDAK
                     KYLRPARRVVRPALKSGTKIERTAYTEKTATRLAYGYALRAAGRVNESIVCLDGDVRN
                     STYADKFFDAFPTRSFQSYIAEQNMIGMATGFAVKGYVPFAASFAAFLSRAHDQIRMA
                     GHSLANLKVCGSHVGVSIGEDGGSQMGLEDIAMFRSIPGATVLYPADAVSAEACVEAA
                     AREKGIFYIRTTRPATPILYPADEAFPVGGSKTLRKSADDAAAVVAAGITLHEALKAH
                     EMLKAEGISIRVVDAYSVQPVDAAGLRAAAAAARNRIIVVEDHYAGGGLGDAVASALA
                     GRTAITHLCIREIPCSGKPAELLDRFGISARRIVEAVKSL"
     gene            complement(2827..3255)
                     /locus_tag="PKV64_08695"
     CDS             complement(2827..3255)
                     /locus_tag="PKV64_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17192.1"
                     /translation="MKKSVSWIAAALLVAAAGAAAAQRLPPGSQAVFLKNGEVLTGRI
                     IDVVLPPLDLKMESGSLIALRDVWMINFESGDWNFPEERNILETNDHYIFLKSGDIHS
                     GRISAYAADRKAFVFETGESFPLVQCRRIYFSKTVPRGLR"
     gene            complement(3317..5305)
                     /locus_tag="PKV64_08700"
     CDS             complement(3317..5305)
                     /locus_tag="PKV64_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HPN17193.1"
                     /translation="MSIFNRRPLQAPRFAARIIFAAVLGLAAAAGTPAAGQNPPDLIL
                     LFTGDVHSAVSPHRIFDEAGRAEGRGGFARIAAMIKAERERHGDRMLVVDVGDMIAGS
                     LFQVIFQPEAAEMRLMKMAGIEVTTFGNHEFDYGSAALARALTTAKVNGAAVPLVASN
                     VAFDPKDGQDDLLEAVFKDYPVDEYLVVDRNGLKIGLMGLMGRNAADDAPAAAPVVFT
                     NMIARARETASILREREKVDLVIALSHAGTTPDPKTSEDEKLAAEVPDIDVIVSGHTH
                     RRLVSPILVGSTIIVGVGCRGEDLGDLRLERTAEGRFRVVSYALRPVTGDIPEDPEVA
                     AEAAKLSARVDLEVEKAYGLDPWEPLAETEIALAMIPPAPINSSWEIGLGDLVTDAYR
                     RSMKTAGARGADGPVLAIHPWGNMRDSLFPGPITGHDVFNVLSLGLGPDGRIGYPLVC
                     IWLTGREIRRAFEVETTIAPGHGDAHLQISGAFVRYNPRRIPFDRVTAIEFEEANGTR
                     RPMEPDRLYPVVANLYLTRMLGYVSRASHGILKIEPKDADGNVRTDFENLIIDGDPAS
                     SGVQEVKEWISLAAMLRSFSDADGDGLAEIPPVYAASAGRLVSEPSWNPVKLLAGAHA
                     PTYVVLGLAAVVLLLAWIVVHIIRRRFARRKARAAAKS"
     gene            complement(5344..6372)
                     /locus_tag="PKV64_08705"
     CDS             complement(5344..6372)
                     /locus_tag="PKV64_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HPN17194.1"
                     /translation="MNIREELEEIEAKTLSPKASLSRDSKGRERTEAQHPFRTAFQRD
                     RDRILHSKSFRRLKHKTQVFLAPFGDHYRTRLTHTLEVSAIARTIARALRLNEDLTEA
                     IALGHDLGHTPFGHAGEETLARLLPGGFTHFAQSLRVVEKLEYDGKGLNLTAEVRDGI
                     YRHSKGRGKILDRRKEDLPVTLEGQIVRVSDVIAYVNHDLDDAVRAGFLKDADIPRPL
                     VAFLGKYHATRIDRMVVDVVESSLKTGLGRIAMSDETMKAVVELRDFLYERVYNHPEA
                     KEELRKTEKIIRDLFRYFVEHPEGYVKDYPAGDPVVIRVGDIVAGMTDRYALALYEQL
                     FFPKSWRT"
     gene            6713..7372
                     /locus_tag="PKV64_08710"
     CDS             6713..7372
                     /locus_tag="PKV64_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPN17195.1"
                     /translation="MGIITENVRAVLAELPPGVELVAAAKTKSPEDILEAIEAGVTII
                     GENYVQEAQAAFAAVGRRARWHCLGHLQSNKVKRAVEIFDMIETVDSAGLAREIGKRA
                     AALGKTMPVLIEINSGEESQKTGVRPAEAEALIGEIARIPNVAVQGLMTMGPFEGDPE
                     ESRPYFRRTREIFEALKKSGIPNVEMRRLSMGMTNSYRVAIEEGATLVRLGTKIFGAR
                     S"
     gene            complement(7483..8415)
                     /locus_tag="PKV64_08715"
     CDS             complement(7483..8415)
                     /locus_tag="PKV64_08715"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HPN17196.1"
                     /translation="MALKKWTRRDFLQTTAVAPIAAAVGCCCTSAKPVPAAPAAAGAA
                     RITSMSRKSRVILVRDAAAVGEGRKIDAVVVQKMLDEAMTTLFAEKDPAAAWARVAGP
                     QDTVGVKTNVWNYLPTGPELEEAIRARLLEAGVRADRISIRDRGVLDDPVFKAATVLV
                     NVRPARVHYWAGMGSCLKNYIMFVPKPSDYHGDACADLAAIWSLPPVKGRTKLNILSM
                     LTPQYHNIGPRGFSEQYLWMYGGLIVGQDVVAVDATGYSIIRAKRLEVFGEDKPLETS
                     AHHIQLADTRHRLGKSDPADIDLVKLGWTENILI"
     gene            complement(8487..9419)
                     /locus_tag="PKV64_08720"
     CDS             complement(8487..9419)
                     /locus_tag="PKV64_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17197.1"
                     /translation="MRIVAAAWLVFTVGGGGVPSMLDSSSEAARLLALLPRESLGWTS
                     GGEDRIFDRETIFEYIDGSGEVYRAFNMRLLASRRFHKAGQADVIIDLFDMGSPADAF
                     GVFTHDLEGEDWGLGQGSLYKGGLLQFWRGRYFVSIFAESENSSTTAVLAELGKKTAA
                     ALGPDGPKPDLVGLVPEEFADGPIRYLHDPQILNYHFYIGPENPLGLGPGVDAVLASI
                     GDRAEKRTFLLVRGRDEAAAESARRGLLRAFVSAGRQDAGAAAGEEGDVFPMKDGRFG
                     AAVRRGRFTAAVFGGTAPEEARAALARSVRPLPR"
     gene            complement(9710..>12396)
                     /locus_tag="PKV64_08725"
     CDS             complement(9710..>12396)
                     /locus_tag="PKV64_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN17198.1"
                     /translation="DEEGNAIPGASLVLTSPNLMGSRTTVSDANGEFRFPALPPGDYA
                     LKAELTGFGTVVRESIRVTTTATLNINMVMKPAAMAEEITIVAEAPTVDVKSTETASV
                     TLSNEILRNVPYNQFTADIVNLAPGVSGNVAYGASENTGIAYTMDGVNVADPEGGSAW
                     VFSDHNVIEEAKIMGVGLPAEYGNFTGVIFNLVTKSGGNELSGHFEFNFQGYKADSKF
                     WQAENTGAYLADYPNLTSPSSKLTDINGHLGGPIIKDKLWFYVGGQFYRTKNRPTGFP
                     EDVDYKQPRFFAKLSAQVDPTLSMSGVFQTTAYNGNNRSASSVRSPEACVTQDSPDWL
                     ASFSLTKILSSNTFFDLKTNYFKGRYYLDPEVGFDPYTHRNLNENMRFFSDGDFYYAD
                     RARFGTNASLTHYAEDFITGSHDFKFGVEVERSMARTRYGYTGRGGELGDNIKYVDYW
                     GYGAFGYLYSGNYLAYQYEGYDTNTRYTRLEVFAQDSWQVSDRLNISLGVRFGQNWGD
                     VKGVDGAVFKSNHLDPRIGFTFDVLGDRTTILKAHYGRFSEAMLTAYHDRMNPLSQFK
                     DYIGYYFIPDDNDPATTGGTWYEWFRITPTEYTMDDNIKHPYMRQYTVGIERELFKDA
                     SFGVTYINRKWDNIIGRYNRAAQYNVIDYYSPELDRTFPIYERTMDTLELSDFIITNL
                     RKGSEYPNILTDIYRKYEGLEILFNKRFSNRWQLLASYVYSKASGTRDNGFGGDIGWG
                     EDPSDPNSYVNAEGCLTNDFTHMLKVQGTYILPLDISLTGYFRTITGFSWTTQVMTGR
                     LNQGQVTFFAEPRGANHYPMAATLDLRLEKIFTLAGKYRLGVIFDVFNVFNDDTITTW
                     GTIAGFDWNAGTYPSTDGHELYTLVRPRQARLGLRLIF"
BASE COUNT         2161 a         3851 c         4152 g         2232 t
ORIGIN      
        1 cttgaccact ccctcccgga gctcttcgat caggccggag acgtcgcggc ggtagagatg
       61 ggcgacaaac agcttcccgg ccgggatgtc gatcgccggc aggcagtcca cgttgtaccg
      121 ggagtagcac tcggggggat tggtgaaaca ctgcttgatc agggattccc ggtcgctgat
      181 cttgtaatcc ttcagcagcg gagtcggggc gaaataaacg aaggagacga gctcggggta
      241 gtcgcaggcg tcaagaaacg tccccaagac cttggggctc agcgaatccc cgcaggtgtt
      301 gacctccatg caggccgact cataggagct cttcccgatc ttctgttttt cccagaaata
      361 gctggcgtgc gattcctcga tggccaggga ttgctcgacc tggctgactt cgatcccccg
      421 ctcggcgatg tagcggtcca taatcgtcac ccgcggatgc atgttgggcc ggccgatcaa
      481 gttagccccg gaggccttga tcaccgcgta gatttcctcg tcggtgaggg tgaccaggga
      541 aacgccgtcg ctgttgtacg cgtggatgat ggtcgccgtg ggcgcgggtt caaatgtctc
      601 gtgtccggtg aaggccatgc cgggctcctc tcatagggat ttgaccgcct cgacgatccg
      661 gcgggcgctg atcccgaaac ggtcgagaag ctcggccggc ttgcccgaac acgggatctc
      721 ccggatgcag agatgggtga tcgccgtccg gccggccagg gcggaagcga cggcgtcgcc
      781 caggccgccg ccggcgtagt ggtcctcgac gacgatgatc cggttacggg ccgcggccgc
      841 ggccgcccgc aaaccggccg cgtcgacggg ttggaccgaa taggcgtcca cgacccggat
      901 ggaaatcccc tcggccttga gcatctcgtg cgctttcagg gcctcgtgga gggtgattcc
      961 ggcggccacg acggcggccg cgtcatcggc gcttttccgc agggttttgg agccgccgac
     1021 ggggaacgct tcatcggccg gatagagaat gggcgtggcc ggacgggtcg tccggatata
     1081 gaagatccct ttctcccggg ccgccgcctc gacgcacgcc tcggccgaga ccgcgtcggc
     1141 cggatagagc accgtcgccc ccgggatcga ccggaacatg gcgatgtcct ccagccccat
     1201 ctgcgaacca ccgtcctcgc cgatgctcac cccgacgtgg gagccgcaga ccttgaggtt
     1261 ggccaggctg tggccggcca tccggatctg gtcgtgggcc cgggagagga aggcggcgaa
     1321 cgaggccgcg aacggcacgt agcccttgac cgcgaagccc gtggccattc cgatcatgtt
     1381 ctgttcggcg atataggact ggaaggaccg cgtcgggaag gcgtcgaaaa acttgtcggc
     1441 gtacgtggaa ttacggacgt cgccgtcgag gcagacgatg gattcgttga cccgtccggc
     1501 ggcccggagc gcgtatccgt aagccaggcg ggtggccgtc ttttccgtat acgccgtccg
     1561 ctcgatcttc gtcccgctct tcagggcggg acggacgacc cggcgggccg ggcggaggta
     1621 ctttttcgcg tcgatctcgg gaatcggccc cagctcggcc aaggccttct cgagctcgtc
     1681 ggccttgagc ggcttgccgt gccagccgtt gaggtcctcg aggaaggaga cgcccttgcc
     1741 cttgaccgtc ttggcgagga cggccgtcgg caagcccttt gatccggccg ccttgagggc
     1801 cgcgaggacg gcctcgatct tgtgcccgtc gatgacgacg gcgtcccagc cgaaggcccg
     1861 gaacttgcgg gccagggcgg cggcgtcgcg ctggtgctgg gtcgggccgc tctggccgag
     1921 gcggttgacg tcgacgatgg cgcagaggtt gcggagcttc atgtccgctc cggagttggc
     1981 cgcctcccag accgatccct cggcgcattc gccgtcgccg cagaggacga agacgcgggc
     2041 gggcgatttg cccaggcgca tcgccgcggc catcccggcg ccgaccgaaa gcccctgccc
     2101 cagggagccg gtggcgacct tgatccaggg catccggggc gtcggatggc cctccaggcc
     2161 gctcgtgatc ttccggaaat cgaggagcgc ttcggccggg aggatccccg cctcggcgta
     2221 ggccgcccag agaatgggcg cggcatgccc cttggacagc acgagctcgt cgttgcccca
     2281 gtcgttcgga tcggccgggt tgaaccgcat cccgccgaaa aagagggcgg tgatgatctc
     2341 ggcggccgag aggcaggtgg tcgggtggcc ggaggccgaa gccgtggtca ttctcatgga
     2401 ctgggcgcgg aggcgcttgg cgatcccatt caaggcatcg atgcggttca tggggattta
     2461 ttctcctcga ggggcgttcc gtcttccgga gggattatat caaatcgcgc gcccggcggg
     2521 gactgccgtt caaagacgat tttctccttt tcaggatccc ggcgggcccg gtttttcaag
     2581 ccgggggcgg ccgccgaatt cggagtcgat cattctcccg gcctgaaaac cggggaccgg
     2641 cccgggcgat tcctcgattc gagaaaaatc caaaaggttg aaccggcgtt ctgcctcggg
     2701 cggacgacat aaggagaccg aaagacgaaa ttcagccaaa cgagcgcggc ggcgctttaa
     2761 ggccgggcgg ctgaaggcgg aaagaaccgg cggcgaagcg aaggacggaa tctccggaac
     2821 gactcgtcag cggaggccgc ggggaacggt cttggagaaa taaatgcggc ggcactggac
     2881 caaggggaaa gactcccctg tctcgaaaac gaaggccttc cggtcggccg cataagccga
     2941 aatgcgaccg gaatgaatgt ccccgctttt caagaaaatg taatggtcgt tggtctcgag
     3001 gatgttccgc tcctcgggga aattccaatc ccccgattca aagttgatca tccagacatc
     3061 gcggagggcg atgaggctcc cgctctccat cttgagatcg agcggaggga ggacgacgtc
     3121 gatgatccgg ccggtgagaa cctccccgtt tttaagaaaa accgcctggc ttccgggcgg
     3181 caggcgctgc gcggcggccg cgccggcggc cgcgacgaga agagcggcgg cgatccaaga
     3241 cacgcttttt ttcatcccgt tctcccggag atcatcttag ccgaaccgct cccgggatgc
     3301 aaatgccggc cggcgctcag gacttggccg ccgcccgggc tttccgccgg gcgaagcggc
     3361 ggcggatgat gtggacgacg atccaggcca ggagcaggac gacagccgcc aggccgagga
     3421 cgacataggt cggggcgtgg gccccggcca ggagcttgac cggattccac gacggctcgg
     3481 agacgagccg gccggcggaa gccgcgtaga caggggggat ttcggccagc ccgtccccgt
     3541 cggcgtcgga aaacgagcgc agcatcgcgg ccagcgagat ccactccttg acttcctgaa
     3601 cgccggagga agcgggatcg ccgtcgatga tgagattttc gaaatccgtc cggacattcc
     3661 cgtcggcgtc cttgggttcg atttttaaaa ttccgtgcga agcccgggaa acgtatccga
     3721 gcatgcgggt gaggtagaga ttcgcgacga cggggtagag ccggtcaggc tccatcggcc
     3781 gccttgtccc gttcgcctcc tcgaattcga tcgccgtgac ccggtcgaac gggatccgcc
     3841 gcggattgta gcggacgaag gccccggaga tctggaggtg agcgtcgccg tggccgggcg
     3901 cgatcgtcgt ttccacctcg aacgcccgcc ggatttcccg gccggtcagc cagatgcaga
     3961 cgagcggata cccgatccgg ccgtccggcc cgaggccgag cgaaaggacg ttgaaaacgt
     4021 cgtggcccgt gatcgggccg gggaacagcg agtcgcgcat gtttccccag ggatgaatgg
     4081 ccaggaccgg cccgtccgcc ccccgcgcgc ccgccgtttt catcgagcgc cgataagcgt
     4141 cggtcaccaa gtcgcccaga ccgatctccc agctggaatt gatcggagcc ggcgggatca
     4201 tggccagggc gatctccgtt tcggccagcg gctcccaggg atcgaggccg taagcttttt
     4261 cgacctcgag atccacgcgg gcgctcagtt tcgcggcctc cgcggcgacc tccgggtcct
     4321 cgggaatgtc cccggtcacc ggccgcaggg cataggaaac gacccggaag cgcccctcgg
     4381 cggtccgctc caagcggaga tcgccgaggt cctcgccccg gcatccgacg ccgacgatga
     4441 tcgtcgaccc gacgaggatc ggagaaacga gccgccggtg ggtatggccg ctgacgatca
     4501 cgtcgatgtc ggggacctcg gcggccagct tttcgtcctc ggaggtcttg ggatcgggcg
     4561 tcgttccggc atgggacagg gcgatgacga ggtcgacctt ctccctctcg cggagaatcg
     4621 aagccgtctc gcgggcccgg gcgatcatgt tcgtgaaaac gaccggggcg gcggccgggg
     4681 cgtcatcggc cgcgttccgg cccatgaggc ccatcaagcc gattttcaat ccgttcctgt
     4741 ccacgaccag gtattcatcg accgggtaat ccttgaagac ggcttccagc agatcatcct
     4801 ggccgtcctt gggatcgaaa gcgacgttgg aggcgacgag cggaacggcg gccccattca
     4861 ccttcgccgt cgtcaaagcc cgggccaggg cggcggaacc gtagtcgaat tcgtggttgc
     4921 cgaaggtcgt cacctcgatg ccggccattt tcatcagccg catttcggcc gcttccggct
     4981 ggaaaatgac ttggaacagc gatccggcga tcatgtcgcc gacatcgacg acgagcatcc
     5041 ggtcgccgtg ccgctcgcgc tccgccttga tcatggccgc gatccgggcg aatccgccgc
     5101 ggccctcggc ccggccggcc tcatcgaaaa tgcgatgcgg cgaaacggcc gaatggacat
     5161 cgccggtgaa cagaaggatc aggtcgggag gattttgacc ggccgcgggc gtcccggccg
     5221 ccgccgccag gccgaggacg gcggcgaaaa tgatccgggc ggcgaatcgc ggggcttgga
     5281 gaggccggcg gttgaaaatg gacatgatcc ctccttgttc ctttttttcg aggcggctcc
     5341 ccatcaggtc cgccatgatt tcggaaagaa cagctgttcg taaagcgcga gagcgtagcg
     5401 gtcggtcatc ccggcgacga tgtcgccgac ccggatcacg accggatcgc cggccggata
     5461 gtccttgacg tagccttcgg gatgctcgac aaagtaccgg aacaggtccc ggatgatctt
     5521 ttccgtcttg cggagctcct ctttcgcctc gggatgattg tagacccgct cgtagaggaa
     5581 gtcgcggagc tcgaccacgg ctttcatggt ctcgtcgctc atggcgatcc gccccaaccc
     5641 ggttttcagg ctggactcga cgacgtccac aaccatccgg tcgatgcggg tggcgtggta
     5701 cttgcccagg aaggcgacga gcgggcgggg gatgtcggcg tccttgagaa atcccgcccg
     5761 gacggcgtcg tcgaggtcgt ggttgacata ggcgatgacg tcggaaacgc ggacgatttg
     5821 gccctcaagc gtcaccggga gatcctcctt gcggcggtcg aggatttttc cccggccctt
     5881 ggagtggcgg tagatgccgt cccggacctc ggctgtcagg ttgaggccct tgccgtcgta
     5941 ttcgagcttc tcgaccaccc ggaggctttg ggcgaaatgg gtgaatccgc cgggaagaag
     6001 gcgggccagc gtctcctcgc cggcgtggcc gaacggagtg tggccgaggt cgtggccgag
     6061 ggcgatggcc tcggtcaggt cctcgttgag gcgcaaggcc cgggcgatcg tccgggcgat
     6121 cgccgacacc tcgagggtat gggtcagccg cgtccggtag tggtcgccga acggagccag
     6181 gaaaacctgg gttttatgct tgagccggcg gaacgacttc gagtggagga tccggtcccg
     6241 gtcgcgctgg aacgccgtcc ggaaaggatg ctgcgcttcg gtccgctccc ggcccttgga
     6301 gtcccgcgat agactggctt tcggagagag agtcttcgcc tcgatctcct ccagctcctc
     6361 cctgatgttc acccggcgat tataccaaaa tttcgagggc cgcgttggat tccgttcgag
     6421 cagccgcggc gccccgccgg acggatcttg gatcggtccg gggaatgacc ggagatgacg
     6481 cgcgagccgc cgatccggac tgactctccg gccggggaaa aggtctcctt tgtccgccgc
     6541 ccgacctgcc cgccgtttgg cggactcctg gacggcttcc cggctctccc ggccgacgga
     6601 ccgcaaaacg ccgggaagtg atcgcccggc gcgggtcgcg aaatctccgg cgtcccttcc
     6661 ccggcctctt cacgacttgg aaggccgggg ggatttcgcc tacaatacgc ccatgggcat
     6721 catcacggaa aacgtccggg cggttctggc cgagcttccc ccgggagtcg agctcgtggc
     6781 cgcggcgaaa acgaagtctc ccgaggacat cctcgaagcg atcgaggccg gcgtcacgat
     6841 catcggggag aattacgtcc aggaagccca ggccgctttc gccgccgtcg gccgccgggc
     6901 ccggtggcat tgccttggcc atctccaatc gaacaaggtc aagcgggccg tcgaaatctt
     6961 cgatatgatc gagacggtcg actcggccgg cctggcccgg gagatcggga agcgggccgc
     7021 ggccctgggc aagacgatgc ccgtcttgat cgagatcaac agcggcgagg aaagccagaa
     7081 aaccggcgtc cggccggccg aggccgaagc cctcatcggc gaaatcgccc gaatcccgaa
     7141 cgtcgccgtc cagggattga tgacgatggg gcccttcgag ggcgatcccg aagagtcccg
     7201 gccttatttc cgccggacgc gcgagatctt cgaagcgctc aagaaaagcg gaatccccaa
     7261 cgtggagatg cggcgcctct cgatggggat gacgaattcc taccgcgtcg cgatcgagga
     7321 aggggccacc ctcgtccgcc tcgggacgaa aatcttcggc gcccgctcct gatccgccga
     7381 acgggacccg tttctcctcg caagagcggc ggcttcagcc tccgccggac gaggcatccc
     7441 ctgccttccg ccgcgccggc cgggatccgg accgggccgg gttcagatta aaatgttttc
     7501 cgtccagccg agcttgacga gatcgatgtc ggccggatcg ctttttccca gacggtgccg
     7561 cgtgtcggcc agctggatgt gatgggccga cgtctccaga ggcttgtcct cgccgaacac
     7621 ctcgagccgc ttggcccgga tgatggaata tccggtcgcg tccacggcca cgacgtcttg
     7681 gccgacgatc agcccgccgt acatccagag gtactgctcg ctgaacccgc gcggcccgat
     7741 attgtggtat tggggggtca gcatcgagag gatattgagc ttcgtcctcc ccttgaccgg
     7801 cggaaggctc cagatcgcgg ccaggtcggc gcaggcgtcg ccgtgataat cggatggctt
     7861 ggggacgaac atgatgtagt tcttgaggca gcttcccatc ccggcccagt aatggacgcg
     7921 ggccgggcgg acgttgacca ggaccgtggc cgccttgaag accgggtcgt ccagcactcc
     7981 gcggtcccgg atggaaatcc ggtcggcccg cacgcccgcc tccagaaggc gggcccggat
     8041 cgcctcttcc agctccggac cggtcggcag gtaattccag acgttggtct tgactccgac
     8101 cgtgtcttgc ggaccggcca cgcgggccca ggccgcggcc ggatccttct cggcgaagag
     8161 cgtcgtcatc gcctcgtcga gcattttttg aacgacgacg gcgtcgatct tgcgcccttc
     8221 gcccacggcc gccgcgtcgc ggaccagaat gacccgcgat ttgcgggaca tcgaggtgat
     8281 ccgggccgcc ccggcggccg cgggcgcggc cgggaccggc ttggcggacg tgcagcagca
     8341 gcccacggcc gcggcgatcg gggcgaccgc ggtcgtctgc agaaaatcgc ggcgggtcca
     8401 ttttttcaaa gccattgtct ttgcctcctt gtatcgatcc atcgaaatcc cgcttttccg
     8461 gaagacaccc gaaggcgcgc cgcgcttcac cggggaagcg gacggacgga ccgggccaga
     8521 gccgcccggg cttcctccgg cgccgttccg ccgaacacgg cggccgtaaa ccgcccccgg
     8581 cggacggccg cgccgaagcg gccgtccttc atcgggaaca cgtcaccctc ttccccggcc
     8641 gcggctcccg cgtcttgccg gccggcggag acgaacgccc ggagaagccc gcgccgggcg
     8701 gattccgcgg ccgcctcatc gcggccgcgg acgagaagaa acgtccgctt ctccgcccgg
     8761 tctccgatcg aggccagaac cgcatcaacc ccgggaccga ggccgagcgg gttttcagga
     8821 ccgatataga aatggtagtt caaaatctgc gggtcatgga gatagcggat cggcccgtcg
     8881 gcgaattcct ccggaacaag gccgaccagg tccggcttcg ggccgtcggg gccgagggcg
     8941 gcggctgttt ttttccccag ttcggccaaa acggcggtcg tcgaggaatt ttccgactcg
     9001 gcgaagatgg agacaaaata acggccgcgc cagaactgga gaagcccgcc tttatagagg
     9061 cttccctggc cgaggcccca atcctccccc tcgaggtcgt gggtgaacac gccgaaggcg
     9121 tcggccggcg aacccatgtc gaagaggtcg atgatgacgt cggcctggcc ggccttgtga
     9181 aagcggcggc tcgccaggag ccgcatgttg aacgcccggt agacttcgcc ggagccgtcg
     9241 atgtattcga aaatggtttc ccggtcgaag atccggtcct ccccgccgga cgtccagccg
     9301 agggattcgc ggggaagaag cgccaggagc cgggcggcct cggaggatga atcgagcatc
     9361 gacgggacac ctcctccccc gaccgtgaag acgagccacg cggccgcgac gattctcatc
     9421 ctttctattt tatcacgacg ggacggaaaa gcggcacccg gctcgataag ctgaatcggc
     9481 ggaagaaaaa gaagccgatc cgcgttcctc ctcataaata gcaagctcct tccagggttg
     9541 tcttttgatt gagtgaacaa tccaagttaa cgttgcctgg ttaggtgagt tggcacaccc
     9601 ggccgccgcc ccggttggaa atcaccgggg acccgggcct tgcatctcca tataaaaagg
     9661 ggcggcgggt ttccccgccg ccccggtgac atgggcgtga acgggaacct tagaagatga
     9721 gccggagacc gagccgggct tggcgaggcc ggacaagggt gtagagctcg tgcccgtcgg
     9781 tggacggata cgtgcccgcg ttccaatcga agccggcgat ggtgccccag gtcgtgatcg
     9841 tgtcgtcgtt gaagacgttg aacacgtcga agatcacgcc caggcggtat ttgccggcca
     9901 gggtgaagat cttctccagc cgcagatcca gggtcgccgc catcgggtag tggttggcgc
     9961 cgcggggctc ggcgaaaaac gtgacctggc cctggttaag ccgtcccgtc atcacttgag
    10021 tcgtccagga gaagccggtg atggtccgga agtagcccgt caggctgatg tccagcggca
    10081 ggatgtacgt cccctggacc ttcagcatgt gggtgaagtc gttggtgaga cagccctcgg
    10141 cgttgacgta gctgttcgga tcgctgggat cctcgcccca cccgatgtcg ccgccgaatc
    10201 cgttgtcccg cgttccggac gccttggagt agacgtagga ggccagcagc tgccaccggt
    10261 tggagaaacg cttgttgaac aggatttcca ggccctcata cttgcggtag atgtcggtga
    10321 gaatgttggg atattccgat cccttccgga ggttggtgat gatgaagtcg ctgagctcga
    10381 gcgtgtccat cgtccgctcg tagatcggga acgtccggtc gagctcgggg gaataataat
    10441 cgatcacatt atactgggcc gcccggttgt accggccgat gatgttgtcc cacttccggt
    10501 tgatgtaggt caccccgaac gaggcgtcct tgaacagctc ccgctcgatc ccgacggtgt
    10561 actggcgcat ataaggatgc ttgatgttgt cgtccatcgt gtattccgtc ggcgtgatac
    10621 ggaaccattc ataccaggtt ccgccggttg tcgccgggtc gttgtcgtcg gggatgaagt
    10681 aataaccgat gtaatctttg aactgggaga gcgggttcat ccggtcgtga taggcggtca
    10741 gcatcgcctc ggaaaaacgg ccgtagtggg ctttcaaaat ggtggtcctg tcgcccagca
    10801 catcgaacgt gaagccgatg cgcgggtcga ggtggttcga tttgaagacc gccccgtcca
    10861 cgcccttgac gtcgccccag ttctggccga agcggacgcc caggctgatg ttcagccggt
    10921 cggacacctg ccaggaatcc tgggcgaaga cctccagccg ggtgtaccgg gtgttggtgt
    10981 cgtagccttc atattgatag gccaggtaat tgcccgaata caggtacccg aaggcgccgt
    11041 agccccagta atcgacatac ttgatgttgt cgcccagctc gccgccccgg ccggtgtagc
    11101 cgtaacgggt ccgggccatc gaccgctcga cctcgacgcc gaatttaaag tcgtgggagc
    11161 cggtgatgaa gtcctcggca taatgggtga ggctggcgtt cgtcccgaaa cgggcccggt
    11221 cggcgtagta gaaatcgccg tcgctgaaaa agcgcatgtt ctcattcagg ttccggtggg
    11281 tgtagggatc gaagccgacc tccggatcga ggtagtaccg gcccttgaag tagttggtct
    11341 tgaggtcgaa gaacgtgttc gagctcagga ttttggtcag gctgaagctg gccagccagt
    11401 ccggcgaatc ctgggtgacg caggcttccg gggagcgcac ggagcttgcg ctgcggttat
    11461 ttccattata agccgtggtc tggaagacgc cgctcatgct caaggtcgga tccacctggg
    11521 ccgagagctt ggcgaagaac cgcggctgct tgtagtcgac gtcctcgggg aatcccgtcg
    11581 gccggttctt cgtccggtag aactgcccgc cgacgtagaa ccacagcttg tccttgatga
    11641 tcgggccgcc caggtggccg ttgatgtccg tcagcttcga ggacggcgag gtcaggttgg
    11701 gataatcggc gagatacgcg ccggtgttct cggcttgcca gaacttcgag tcggctttgt
    11761 agccttggaa gttgaactcg aagtggccgg agagttcgtt gccgccggac ttcgtcacca
    11821 ggttgaagat gacgccggtg aagttgccgt actcggccgg cagccccacg cccatgatct
    11881 tggcttcctc gatgacgttg tggtcggaga agacccaggc cgagccgccc tcgggatccg
    11941 ccacgttgac gccgtccatg gtgtaggcga tccccgtgtt ctccgaggcg ccgtaggcga
    12001 cgtttccgct gactcccggg gcgaggttga cgatgtcggc cgtgaattgg ttgtagggga
    12061 cgttccgcag aatctcgttg ctcagggtga ccgaagccgt ctccgtggat ttgacgtcga
    12121 cggtgggggc ttcggccacg atcgtgattt cctcggccat ggccgccggc ttcatgacca
    12181 tgttgatatt gagggtggcg gtcgtcgtca cccggatact ttcccgaaca acggtcccga
    12241 agcccgtcag ctcggctttc aaggcgtaat cgccgggcgg cagggccggg aaccggaatt
    12301 ccccgttggc gtccgaaacc gtggtccggg agcccatcag gttgggactg gtcaggacca
    12361 ggctggcccc cgggatcgcg ttgccttcct cgtccg
//
LOCUS       DAOOXH010000074        12211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_5425_length_12211_cov_6.901612, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000074 DAOOXH010000000
VERSION     DAOOXH010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 12211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12211
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5425_length_12211_cov_6.901612"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PKV64_08730"
     CDS             complement(<1..798)
                     /locus_tag="PKV64_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17199.1"
                     /translation="MRNVYLLFAPSAEIKVKRLIMPLALLPVAASIRRSPLFKSGDRV
                     YVCDSLREVLERAGSHAGDRVHFLVSSTRVIRGLERPTNPAVECAARLRRHRPDVFLG
                     VGGPDVFLDPGAYRPSFDLVFQGEVGSVDLLEVIRSGVPFFQAPPADINAEPLDYRPL
                     SGKKYLAGSLQTARGCPHDCSFCNVAHFSGRAVRRLAPRLLEERLESLADIHRGFVII
                     GDSNFAGGSAAAMKEYLDVLYRVQERRGFPFLVGLQASLQTSRHPDIL"
     gene            1094..1672
                     /locus_tag="PKV64_08735"
     CDS             1094..1672
                     /locus_tag="PKV64_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPN17200.1"
                     /translation="MMPTRDAWYTQHYPLMQDYERTLYRTLSEAGRLGFQELFWTSRY
                     PDAIKLFQERLDFIVKAYKRENARQPWNTDRGRIYILNGPPAAVDIDQNTDWGAQVTQ
                     GGAATGAWAVDRSNEDVQAFRTEIWTYSVRSQYAKYVFVFRAPNEWKLSAAAVEGNQF
                     ISLLENYNKTVTFGPRDEAEYRKALDALEKKK"
     gene            complement(1833..4877)
                     /locus_tag="PKV64_08740"
     CDS             complement(1833..4877)
                     /locus_tag="PKV64_08740"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPN17201.1"
                     /translation="MGKYRNYFLLAVCVLLVTSMLPAQRTTGQIFGKVVDDNGAALPG
                     VSVVAEGPRLVGKATDITNALGAYRLLALPPGTYTIEYTLEGFKPLVRKDVVIAAEMT
                     VTLDITMNASTIEEQITVVGKSPLIDVKSTTRGAAMNRQVFTLLPKGRNFDSLLVTVP
                     GVQSESLLAGTSVDGASGAENMWYVDGISTTNLINGTSGQGVNFDFVEEVNFKSSGYN
                     AEYGGSLGGVINVLTRSGGNEFHGEVLAYYTGEPVTARRRDVLVYNKYDTRYDQSAMY
                     YAWEDYYGKQKWYNLEGGLNIGGYIIKDKLWFFASAIPNYNNYTRTLNYAIQGLTNPS
                     RDVHQKSLDLNAQVKLSAQPLKNLRLTASFINNMSQVRGGATGFPSSYTGSTTLDYDI
                     YDFDYPNWSASASADWIVTSNFMVSVRGGFFRTNQNNQVPEATTSPWYGFQMEQPYSY
                     AFSDPSAFPEIPESLVHAPGWFNYPRANLLGYAKTIRQKMNFNVDLSYFATFAGEHAF
                     KIGGQFIRQGEDVDQGGQAPIVYIAWDQDLEIYGDVYNRGKYGWYAVRGNKDSGPFGS
                     VYNAFSNQFAFYVQDSWTIGQKLTINLGVRTESEYIPSYSQDPDYKDLKPIKFSFDKK
                     ISPRFGVIYDVFGDSSLKVFGSFAIYQDVMKLDVAANAFGGFKWKSAYYTLDDYDYTK
                     IGVNGYFPGTLQAVFDFRAPSFDTVDPDIRPFTQREIAFGAEKKLGEDFSVSLRLVNK
                     SVLYAVDDTAVVLPGVGEMYFYTWIGSPFIAQKYKEAKAAGLLPEAVPDQPKAKREYW
                     ALNFSFDKRLSNNWLMGFAATISSLRGNYSGLANSDEQGRNNPNGERSFDLWHFQYDL
                     NMDLIDGPLATDRPIVLKLYGSYVFPMGLTVGAVMQAMSGTPITTAWNVDGPGYYPYN
                     RGDLGRTPFLFYTDAYAEYNFKLGSKYNLQLSVNLSNVFNVETATFISGNVYRTNITP
                     GDDVLVKGNWTPNPAAKKDGYYLKASSFFAPMQARFGVKFSF"
     gene            5513..7615
                     /locus_tag="PKV64_08745"
     CDS             5513..7615
                     /locus_tag="PKV64_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPN17202.1"
                     /translation="MKRSLAFRVSCFVLLLISSLVLLPPVPVLAQAGKKKVTDLPEHY
                     RKWLEEEVVYIITPRERDVFLQLQSDREREVFIKAFWRQRDPTPNTEKNEFREEHARR
                     IAYVQQWFGRGTAGAGWRSDMGRIFIILGEPKTIDRYENLAQVFPTVVWFYDGMERSG
                     LPNSFNVVFFKEEGTGNYVLYSPMKHGPQKLLNNYMGDVTDYESAYRQMFDIQPEIAN
                     VSLSLIPGEHLLVPRPSMSSEILLSSRIPSAPYEKIKDEYAEKLLLYKDIIEVDYTAN
                     YIESDSLVRVYQGPAGDAFVHFLVEPKRLSFEPVEDRYHADLETDVSVTDPQGTVVYQ
                     FTRSVPFDMTESQLAAVRDKLFSFQDVFPLVPGSYRLNILLKNRVSREFTSAEASLII
                     PAPGAFTLYAPAISNRLDSAAKFRGQTKPFVFSGLQLTPSPRNEFLPGETLHAFFQLQ
                     NIPAEVLRGGFIEYTVVRETENGPQVHQTVTKNIADYPDPANIFESFPMAGWPAAYYT
                     LRISIQNAARAPQVTAKEQFLITALPVLKRPWVLSVPQPSPDAPVFSRIIGIQYLNRK
                     DFDLARPRLEAAFRSDPASIPFALDYIRLLIEIKDYAGAKAAVQPFLADDRKWDFLEY
                     AGRTCQALGENAEAIAHYKDYLKHFGTNLNVLNAVGDCYAADGNVPEALAAFENSLRI
                     EPNQPKIKDKIEELKRKK"
     gene            7612..8520
                     /locus_tag="PKV64_08750"
     CDS             7612..8520
                     /locus_tag="PKV64_08750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPN17203.1"
                     /translation="MNNQGRRSAVLILALAGFASACITARVINSLDAESRDFLSKVRY
                     LITKQERQRFLAIPAGDRTSFMEEFWEKRESEKLPGEKSFKEEYFARIEEANRLFSEG
                     SEPGWLQDRGRVYILLGPPTNRITYPRGVTFWGVPTEYWYYGFFPLTFTDAWWNGNYK
                     LDPESVEQVAILNRTQKDWQPYVGPEEGRIEFKVKVERPKPGAALIEIAVPYRDIRFE
                     IKNRVMRAILVLTLDLLEKDENVIRQYQAEYPIEIADADFDRVKAEDYVLNVPMEASP
                     GEYGLRLILKNAKDGIKTYKKIKISL"
     gene            8708..9682
                     /locus_tag="PKV64_08755"
     CDS             8708..9682
                     /locus_tag="PKV64_08755"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN17204.1"
                     /translation="MKTKILRTTALGLSVVFLASGLIYAQAGRGTAFLSGNVTDEEGN
                     PLEGAQVTLSFVQGGGGVKQEALTDKKGEWSFLGLGTGQWNLAVVMKGFEPSAQAVTI
                     SQLSRNAKVWIKLKKIIGGADGAVLQDEDSLVFLDEGNQYYKDGDYDEAIARYELFLE
                     KNPKAYQVQVNIADAYKEKGDYEKAKELYDKIIDLSANDATLGREVSGKAYAGIGDIF
                     LKQNKLAEAQEYFRRSIERSPKDEVLAYNVGEIYFSNQNLPEAQKYFELAMQIRPEWP
                     DPYLKMGYVFLNTGDNEGAIEMFEKFLTLETEGERATMVHNVLSALKK"
     gene            9837..10700
                     /locus_tag="PKV64_08760"
     CDS             9837..10700
                     /locus_tag="PKV64_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17205.1"
                     /translation="MNGRPARRSRVLRSVVAALFAAAWPASFAAAQFLPDEIARRAEC
                     EEFLRTAEIVEVRAIGEGITRPKKVILRKGGIEKTAVWKNPEERDGDTLDQWRFEIAA
                     YRLDKLLGLNMVPPTVERSLDGRPGALSLWVGRTLNLVQVVEQNIVIPPEAEGAFNDM
                     KYVARAWDCLIANDDRTQQNVLYTSDWRTILIDHSRAFGCRGEYGRRLVFGLNGIKTA
                     EDGRPFLFRRLPRRFVRAIERLDLDAVREAVGRYLKNDEIKAVLKRRDILLREIQAMI
                     ADQGEDRILYD"
     gene            10693..11742
                     /locus_tag="PKV64_08765"
     CDS             10693..11742
                     /locus_tag="PKV64_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17206.1"
                     /translation="MTDRRRDLRLGGAPPAREGTPRDASKGRPWQTKNGPVINKSGFN
                     WIGILLLAGIGGTAGVVSWSVDSTKASALEIVSAGRPPAATAVAPARTSAQDAPIVEI
                     AEAAAWEKFLREAEITGQERISGEQAVTSPWRLTLALGGVERYGLWKNPEGRLGGFIE
                     GWQYEIAAYLLDRHLRLNMVPPTVERRFQGERGSLQIWIDGCMTALEKEEKKLRIPGS
                     RLLDWNRATYLQRAFDNLIGNYDRHLNQVLVTPDWHLILIDHSRAFAASKFDKRLIYT
                     EKHREGPKLMRQLPKGFVERLKTLDFETIRAATGGCLNPSEIRNMLVRRDLITAEIDR
                     LIGLYGADGPEGVLY"
BASE COUNT         2570 a         3642 c         3658 g         2341 t
ORIGIN      
        1 gaggatatcg ggatggcggg atgtttgcag ggacgcctgg aggccgacga ggaagggaaa
       61 cccccttcgc tcctgaaccc ggtaaaggac atccaggtat tccttcatgg ccgcggccga
      121 cccgccggcg aaattggaat cgccgatgat gacgaatcca cggtggatgt cggccagcga
      181 ttccagccgc tcttcgagaa ggcggggggc gagcctccgg acggcccggc cggaaaaatg
      241 agcgacgttg cagaacgaac agtcgtgggg gcagccccgc gcggtctgaa gcgatccggc
      301 caggtatttc ttcccggaca aggggcggta atcgagcggc tcggcgttga tgtcggccgg
      361 cggggcttga aagaaaggaa caccggaccg aatgacctcg agaaggtcga cgctcccgac
      421 ctcaccctgg aagacgaggt cgaaggacgg ccggtaggcc ccgggatcga gaaaaacatc
      481 cgggccgccc acgccgagga agacatccgg gcgatgccgg cgaagacggg cggcgcattc
      541 aacggccggg ttcgtcggcc gttccaagcc ccggatgacc ctcgtggatg aaaccaaaaa
      601 atgaacgcgg tcgccggcgt gggagccggc ccgctcgagg acttcccgca acgagtcgca
      661 gacatagacg cggtcgccgc tcttgaagag gggcgagcgg cggatggagg cggcgacggg
      721 cagcaaggcc aggggcatga tcagccgctt gaccttgatc tcggcggacg gggcgaagag
      781 aaggtagacg tttcgcatgg agcagccgga ataaagcgtt ccgatgatat cggacgccgc
      841 ctcccggagt caaaccgggg gaaaggatct caagccttga cttctctttc ttgccgatcc
      901 atcatcatga tgtgaagcat ccccggagaa gggaattgcc acaggctttt taaacgcacg
      961 gcctctttct tgaaccggcg ggaacgggaa aaggagacaa atcatgacga atcgcaaaag
     1021 ggatggaaaa tttctttggg gcgcaggggg aattttggcc gttgtcatga taatgaactc
     1081 atgcggaatc aacatgatgc cgaccaggga cgcgtggtac actcagcatt atcccctcat
     1141 gcaggattac gaaaggactc tctaccggac cctgtcggag gccggccggc tcggattcca
     1201 ggagcttttc tggacgtccc gctatcccga tgccatcaag ctgttccagg aacgcctgga
     1261 cttcatcgtc aaagcctaca agagggaaaa cgcccgtcag ccttggaaca ccgaccgcgg
     1321 gcgcatctac atccttaacg gtcccccggc cgccgtcgac atcgaccaga acacggattg
     1381 gggggcccag gtcactcagg gaggcgcggc cacgggggct tgggccgtcg accgcagcaa
     1441 cgaggacgta caggctttcc ggaccgagat ctggacgtat tccgtccgga gccagtacgc
     1501 caagtatgtc tttgtcttca gggctcccaa tgaatggaag ctgtcggcgg ccgctgtcga
     1561 gggcaatcag tttatcagcc ttcttgaaaa ttacaacaaa acggtgacgt tcggtccgcg
     1621 cgacgaggcc gaataccgga aagccctgga cgctctcgaa aagaaaaaat agcccgggcg
     1681 cggtttccct cccatcctca gacagggggc tttccgcctt ccgtggccgt tgaagacgcg
     1741 acgacccggc cgggaatccg ggaaacaaaa aaaaacccgc cccggccgaa gccggggcgg
     1801 gcaagagtag gagtaggagg agttcgctca gcttagaagc tgaatttcac tccgaaccgg
     1861 gcctgcatgg gggcgaagaa ggagctggcc ttaaggtaat agccgtcctt cttggcggcc
     1921 ggattaggcg tccagttgcc cttcacgagg acatcgtcgc ccggcgtgat gttggttctg
     1981 tacacgttgc cgctgatgaa ggtcgccgtc tcgacgttga acacgttgct caggttgacg
     2041 ctgagctgga ggttgtactt gctgcccagc ttgaagttat attccgcgta ggcatcggtg
     2101 tagaagagga agggagtccg cccgagatcg ccgcggttgt acgggtagta acccgggccg
     2161 tcgacgttcc aggccgtcgt gatcggggtt ccggacatcg cctgcatgac cgcgccgacg
     2221 gtcagcccca tcgggaagac gtagctgccg tagagcttga ggacgatggg ccggtcggtg
     2281 gcgagcggac cgtcgatcag gtccatattc aggtcgtact ggaagtgcca caggtcgaac
     2341 gaacgttcgc cgttggggtt gttgcggccc tgctcgtcgg agttggccaa accggaatag
     2401 ttgcccctca ggctgctgat cgtcgccgcg aagcccatca gccagttgtt gctcagccgc
     2461 ttgtcgaagc tgaagttgag ggcccagtac tcgcgcttgg ccttcggctg atccggcacg
     2521 gcttcgggca gaaggcccgc ggccttggct tctttgtact tctgagcgat gaacggggag
     2581 ccgatccagg tgtagaagta catttctccg acgccgggga gaacgaccgc cgtgtcgtcg
     2641 acggcataaa ggaccgactt gttgacaagg cggagggaca cggagaagtc ctcgccgagc
     2701 ttcttttcgg cgccgaaagc gatttcgcgc tgggtgaagg gcctgatgtc gggatcaacg
     2761 gtgtcgaagg agggagcccg gaagtcaaaa acggcctgaa gcgttccggg gaagtagccg
     2821 ttgacgccga tcttcgtgta gtcgtagtcg tccagggtgt agtaagcgga tttccatttg
     2881 aagccgccga aggcgttggc cgcgacatcg agcttcatga cgtcctggta gatggcgaac
     2941 gagccgaaga ccttcaagct ggagtcaccg aagacatcat aaatgacgcc gaaccgcggg
     3001 gaaatcttct tgtcgaagga aaacttgatg ggcttgaggt ccttgtagtc cggatcctgg
     3061 ctgtagctcg ggatgtactc ggactcagtc cggacgccca ggttgatcgt cagcttctgg
     3121 ccgatggtcc agctgtcctg gacataaaag gcaaactggt tgctgaacgc attgtaaacc
     3181 gagccaaagg ggccggagtc cttgttgccg cggacggcgt accagccgta tttgccgcga
     3241 ttatacacgt ccccgtagat ttccagatcc tggtcccagg cgatgtaaac gatgggggcc
     3301 tggccgccct ggtcgacgtc ttcgccctgc cggatgaact ggccgccgat tttgaaggcg
     3361 tgctcgccgg cgaacgtggc gaagtaggaa aggtcgacgt tgaagttcat cttctggcgg
     3421 atggttttag cgtagcccag gaggttggcc cgggggtaat tgaaccagcc gggagcatga
     3481 acgagcgatt ccgggatttc aggaaacgcg gaaggatcgg agaacgcgta gctgtacggc
     3541 tgttccatct ggaaaccata ccagggcgac gtcgttgctt cggggacttg gttgttctgg
     3601 ttcgtccgga agaagccgcc gcgcacgctg accatgaagt tgctcgtaac gatccagtcg
     3661 gcgctggcgc tggcgctcca gttcggataa tcgaaatcgt agatgtcgta gtcgagtgtc
     3721 gtgctcccgg tgtaggagct ggggaagccg gtcgcgccgc cgcggacctg ggacatgttg
     3781 ttgatgaagc tggcggtcaa gcggaggttc ttgagcggct gggcggacag cttgacctgg
     3841 gcgttgaggt ccaggctctt ctggtggacg tcccgcgagg gattcgtcag cccctggatc
     3901 gcgtagttga gcgtccgggt gtagttgttg tagttgggga tggccgaggc gaagaaccac
     3961 agcttgtcct tgatgatata cccgccgatg ttcaggcctc cctcgaggtt gtaccacttc
     4021 tgcttgccgt agtaatcctc ccaggcgtaa tacatggccg actgatcgta gcgggtgtca
     4081 tacttgttgt aaacgaggac gtcccggcgc cgggccgtca ccggctcgcc ggtgtagtag
     4141 gccagcactt cgccgtggaa ctcgtttcca cccgatcggg tcaggacgtt gatgacgccg
     4201 cccagcgagc cgccgtattc ggcgttgtaa ccggacgact tgaagttgac ctcctcgacg
     4261 aagtcgaagt tgacgccctg gccgctcgtc ccgttgatca ggttggtcgt gctgatcccg
     4321 tcgacatacc acatattctc ggcgcccgaa gccccgtcga cggatgttcc ggcaagcagg
     4381 gattcgctct ggacgccggg cacggtgaca agcagcgaat cgaagttcct gcccttgggc
     4441 agcagggtga agacctgccg gttcatggcc gcgccgcgag tcgtgctctt gacgtcaatg
     4501 agcggggact tgccgacgac cgtgatctgt tcttcgatcg tgctggcgtt catcgtgatg
     4561 tcgagggtca cggtcatttc ggccgcgatg acgacatcct tgcgaacgag gggcttgaac
     4621 ccctccaacg tgtactcaat cgtgtaggtc cccgggggaa gagccagcag ccggtacgcg
     4681 cctaaagcgt tcgtgatatc ggtggctttg ccgaccagcc tgggtccctc ggcgacgacg
     4741 ctgacaccgg gaagggcggc tccattgtcg tccacaacct tcccaaagat ttgtcccgtc
     4801 gtccgctggg ccggaagcat gctcgtgacg agcagcacgc atacggccag caggaaataa
     4861 ttcctgtact ttcccataca ttctctcctt ttaatctagg ttccctccaa ggatgcgatt
     4921 gataaacgct ttccttggaa ctttcgcgcc gtttcccctt acggaaacgg cgaccccggc
     4981 cgtttctacc gtaatggtct ccccgtcatt tccctcctca agttgaattc ctgctgagcc
     5041 atcttgcttt cttccccgat caaattactt cttttaagtc tgcgattata ctcaagcaat
     5101 aatcgtgcca atcaaggaat tcgaaaaccg ggcgatttca acggaattat ttcaatgttt
     5161 acagggggaa agccgatttg atcgatttcc cggcgtctga tcttgaggat tataaaacct
     5221 aagaaatcca atcggctgaa tctcgatcca atattttgga tacaagacgg tgaatctttc
     5281 tatcttcgcc tctcataaat ttcaactcag ccaagccgaa aagcgcgcct ttcccgtttc
     5341 gaatccggcc cgctgagttt ctattccacc tcgattccga atcgcttcgc tgagcggaac
     5401 cggccggccg cgtcccgcca tccgaaaaag caacgaatgg cgaaccgacg gcgttaataa
     5461 aggaaaagcc ggcctcgaaa ttttccggga atgtgataaa atacaaaaaa ccatgaaacg
     5521 atcgcttgct tttcgtgtca gttgtttcgt tcttcttctc atctcctcgc tggtcctgct
     5581 ccccccggtt ccggtcttgg ctcaggccgg aaagaagaag gtgacggacc ttccggagca
     5641 ctaccgcaag tggctcgagg aagaggttgt ctatatcatc acgccgcggg agcgcgacgt
     5701 ttttctccaa cttcaaagcg accgcgagcg cgaagtcttc atcaaggcgt tctggaggca
     5761 gcgcgatccg acgcccaaca cggaaaagaa cgagttccgg gaggaacatg cccgccggat
     5821 cgcctatgtt cagcaatggt tcggccgagg cacggccggg gccggctggc ggtcggacat
     5881 gggccgcatc ttcatcatcc tcggggagcc caagacgatc gaccgctatg agaacctggc
     5941 ccaggtcttc ccgaccgtcg tctggtttta cgacggcatg gagagatccg ggctgcccaa
     6001 tagcttcaat gtcgtcttct tcaaggagga agggacaggc aactacgtcc tctacagccc
     6061 gatgaagcac gggccgcaga aactcctgaa caactacatg ggcgacgtga cggattacga
     6121 gagcgcttac cggcagatgt tcgatatcca gccggagatc gccaatgtct ccctttccct
     6181 gatccccggc gagcacctgc tcgtgccccg gccgtcgatg tcctcggaga tcctcctcag
     6241 cagccgcatc ccctccgccc cgtacgaaaa gatcaaggac gagtatgccg aaaagctcct
     6301 cctttacaag gacatcatcg aagtcgatta cacggccaac tacatcgaaa gcgactcgct
     6361 cgttcgggtc tatcaaggcc ccgcggggga tgcgttcgtt catttcctcg tcgaacccaa
     6421 gcgcctgtcg tttgagcccg tcgaagaccg ctatcacgcc gacctcgaga cggatgtttc
     6481 cgtgaccgat ccgcagggca cggttgttta ccagttcacc cggtccgtcc ccttcgacat
     6541 gaccgagtcg cagctggcgg ccgtccgcga taagctcttc agctttcagg atgtttttcc
     6601 gctggtgccc ggttcttacc gcctcaacat ccttctcaag aaccgcgttt cgcgcgaatt
     6661 cacctcggcc gaagcgagcc tcatcatccc ggcccccggc gcgttcacgc tttacgcccc
     6721 ggccatttca aaccggctcg actcggccgc caaattccgc ggacagacta agcccttcgt
     6781 cttcagcggg cttcaactga cgccctcgcc gcgcaacgag ttccttcccg gagaaaccct
     6841 ccacgccttt ttccagcttc agaacatccc cgccgaagtc ctccgcggcg gattcatcga
     6901 gtacaccgtc gtccgggaaa cggaaaacgg cccgcaagtc caccagaccg tgacaaaaaa
     6961 catcgccgat tacccggacc cggcgaatat tttcgaatcc ttccccatgg ccggctggcc
     7021 ggccgcctac tataccctcc gcatttccat ccagaacgcc gcccgcgctc cgcaggtcac
     7081 ggcgaaagaa cagttcctga tcaccgccct gccggtcctg aagcggccct gggtcctgtc
     7141 cgttccccag ccgtcgccgg acgcccctgt cttttcccgc atcatcggga tccaatatct
     7201 gaacaggaag gattttgacc tggcccggcc ccggctcgaa gccgctttcc gaagcgaccc
     7261 ggcgtcgatt ccctttgctc tcgactacat ccgtctcctc atcgaaatca aggactatgc
     7321 cggcgctaaa gccgccgttc aaccgttcct cgccgatgac cgaaaatggg acttcctcga
     7381 gtacgccggc cggacctgcc aagccctcgg tgagaacgcc gaggcgatcg cccattacaa
     7441 ggactatttg aaacacttcg ggacgaacct gaacgtcctc aacgccgtcg gggactgcta
     7501 tgccgcggac gggaatgtcc cggaagccct cgccgccttc gagaattcct tgaggatcga
     7561 gccgaaccag ccgaaaatca aggacaaaat cgaagagctc aagcggaaaa aatgaacaac
     7621 caggggaggc gttcggccgt cctcattctg gcgttggcgg ggttcgcctc cgcttgtatc
     7681 accgcccgag tcatcaattc gctggacgcg gagagccggg atttcctgtc caaggtccgc
     7741 tacctcatca cgaagcagga gcggcaacgg ttcctggcca tccccgccgg ggaccggacg
     7801 tcgttcatgg aagaattctg ggagaagcgc gagtccgaaa agctccccgg agaaaaaagc
     7861 ttcaaggaag aatacttcgc ccgcatcgaa gaggccaacc gcctgttttc cgagggctcc
     7921 gagcccggct ggctccagga ccgcggccgg gtttacatcc tgctcggtcc gccgacgaat
     7981 cggatcacgt acccccgggg agtcacgttc tggggcgttc cgaccgagta ttggtactac
     8041 ggatttttcc cgctcacgtt caccgacgcc tggtggaacg gcaactacaa actcgatccg
     8101 gaaagcgtcg agcaagtcgc gatcctcaac cggacccaga aggattggca gccctacgtt
     8161 ggtcccgagg aaggccggat cgagttcaag gtcaaggtcg agcggccgaa gccgggcgcg
     8221 gccttgatcg aaatcgccgt cccgtaccgg gacatccggt tcgagatcaa gaaccgggtc
     8281 atgcgggcga tcctcgttct gaccctcgat ctcctggaga aagacgagaa tgtgatccgg
     8341 caataccaag ccgaataccc gatcgaaatc gccgacgccg atttcgatcg ggtgaaggcc
     8401 gaagattacg tcctgaacgt cccgatggag gcatcgccgg gtgaatacgg actccggctg
     8461 atcctcaaga acgccaaaga cggcatcaaa acctataaaa agatcaagat ctcgctttga
     8521 tccatcctcc agagggagaa aaacgacgtc ccgagccagg caaaagtgat ataatgtttt
     8581 tcctgaggtc gatgaccggg caagcagaag aggaattcgg gaccggaaat ccgttttccc
     8641 ctgaagcccc tcaagggact gaatggcatg gtttttgcgt atgatgcatg atggatggag
     8701 gacacaaatg aaaacgaaaa tcctgcggac gacggccctc ggtctttcgg ttgttttcct
     8761 cgcgtccggc ctgatttacg ctcaggcggg acgcgggacc gcctttctca gcggaaacgt
     8821 caccgacgag gagggcaatc cccttgaggg cgcccaagtc accctgagct tcgtccaggg
     8881 cggcggcgga gtgaagcagg aggccctgac cgataagaaa ggcgagtggt cgttcctggg
     8941 cctcgggacg ggccaatgga acttggcggt cgtgatgaaa ggcttcgagc cctccgccca
     9001 ggccgtgacg atctcccagc tttcccgcaa cgccaaagtg tggatcaagc tgaaaaaaat
     9061 catcgggggc gcggacgggg ccgtcctcca ggacgaagac tcgctcgtct ttctcgatga
     9121 gggaaaccag tattacaagg acggcgacta cgacgaggcc atcgcccggt acgaattatt
     9181 cctggaaaag aatcccaagg cctaccaggt ccaggtcaac atcgccgacg cttacaagga
     9241 aaagggcgat tacgaaaagg cgaaggagct ttacgacaag atcatcgacc tttccgccaa
     9301 cgacgcgacc ttgggccggg aggtttcggg gaaggcttac gccggtatcg gcgacatctt
     9361 tttgaagcag aacaagctcg ccgaggccca ggaatatttc cggcggtcca tcgagcgttc
     9421 gcccaaggac gaggtcctgg cctacaacgt cggcgaaatt tatttctcca accaaaacct
     9481 ccccgaagcc cagaaatact tcgagctggc catgcagatc cggccggagt ggcccgaccc
     9541 gtatcttaag atgggctacg ttttccttaa taccggcgac aatgaaggcg cgatcgagat
     9601 gttcgagaag ttcctgacgc tcgaaaccga aggagaacgg gcgacgatgg tccacaacgt
     9661 cctttccgcg ctgaaaaagt aacctcctga tcgcggccgc cgcggaagaa agacggctcc
     9721 tcggtttcgc cggccgtccg catccgggcg cgtcgtcggc gggcgcatct taagaggtcc
     9781 ggagcgcgtc ctcgtctttg ttgactcccc cggggataaa gcctataaat ttgactatga
     9841 acgggagacc agcccgccgg agccgggttc tccggagcgt cgtcgccgcc ctcttcgcgg
     9901 ccgcctggcc cgcctctttc gccgcggccc agttcctgcc cgatgagatc gcccgccggg
     9961 ctgaatgcga ggaattcctg cggacggcgg agattgtcga ggtcagggcc atcggtgaag
    10021 gcattacccg cccgaagaaa gtgatcctcc gcaagggggg gatcgaaaag acggccgtct
    10081 ggaaaaatcc cgaggagcgc gacggcgata ctttggacca gtggcgcttc gagatcgccg
    10141 cctaccgtct ggacaagctg ctcgggctca atatggttcc gccgaccgtc gaacgcagcc
    10201 tggacggccg ccccggcgcg ctctctctct gggtcggccg cactctcaac ctggttcagg
    10261 tggtcgagca gaacatcgtg atcccccccg aggccgaagg cgctttcaac gacatgaaat
    10321 acgtcgctcg ggcctgggac tgtctgatcg ccaacgacga cagaacccag cagaatgttc
    10381 tctacacctc cgactggcgg acgattctga tcgaccacag ccgggctttc ggctgccgcg
    10441 gggagtacgg gcgccgcctg gtgttcggct tgaacgggat caagacggcc gaagacgggc
    10501 ggcccttcct cttccgccgg ctgccccggc ggttcgtccg ggcgatcgaa cggctggatc
    10561 tcgacgccgt ccgcgaagcc gtcggccgct acctcaagaa tgacgagatc aaggccgtcc
    10621 tgaagcggcg ggacatcctt ctgcgggaaa tccaagccat gatcgcggat caaggcgagg
    10681 atcggatcct gtatgactga ccggcgccgc gatcttcggc tgggaggcgc gcctccggcg
    10741 cgtgaaggga ccccccggga cgcctcgaag ggccggccgt ggcagacgaa gaacggcccg
    10801 gtgatcaaca aatctggatt caactggatc ggcatcctcc ttctcgccgg aatcggcggg
    10861 acggccggcg tcgtctcctg gtcggtcgat tcgacgaagg cctccgccct ggagatcgtt
    10921 tccgccgggc gtccgcctgc ggcgacagcc gtcgcgccgg cccggacgag cgcccaggac
    10981 gccccgatcg tcgaaatcgc cgaggcggcg gcctgggaaa agtttttgcg cgaggccgag
    11041 atcaccggcc aggaacggat ttcgggagaa caggccgtga cgagtccttg gaggctcacc
    11101 ctcgcgctcg gcggcgtcga gcgttatgga ctctggaaga atcccgaggg ccggctgggg
    11161 gggttcatcg aaggctggca gtacgagatc gccgcctacc ttctcgaccg ccatcttcgc
    11221 ctgaacatgg tcccgccgac cgtcgagcgg cggtttcaag gggaacgggg cagccttcag
    11281 atctggatcg acggctgcat gaccgcgctc gaaaaggaag aaaagaagct gagaataccc
    11341 ggctcccggc tcctcgactg gaaccgggcg acctatctcc agcgggcctt cgacaatctc
    11401 atcggcaact acgaccggca cctgaatcag gtcctcgtta cgccggattg gcatctcatc
    11461 ctgatcgatc attcccgggc cttcgccgcg tccaagttcg acaagcgcct catctatacc
    11521 gaaaagcacc gcgagggacc gaagctgatg cgccagctcc ccaagggctt cgtcgaaaga
    11581 ctgaaaacgc tcgatttcga aaccatacgc gccgcgaccg gcggctgctt gaatccatcc
    11641 gagatccgga acatgcttgt ccgtcgcgat ctcataacgg ccgagatcga ccggctgatc
    11701 ggcctttacg gggccgacgg ccccgagggt gttctctatt gaggcgcggg cggagagccg
    11761 ctcgattcct cgcgacgccc ttcagatcat cacaagagcc gcggccgatc cgccgcggga
    11821 caaggcgccg cggccgcctt gcgtccaggg aagccggcct tgatcccgat ctgcctgtct
    11881 gattttccgt aagatataat tcattccgaa ggctagtgta ccgtgcggaa gactggaata
    11941 gcgagaggag ctgcattcat gaagcgaaaa tcaacggatc cttggataag ttttacgagc
    12001 tgaccgcgaa aatctcatgt taaagagagt cacgatggct cccaaaatgc aaacatccgt
    12061 tgacacggcc cctcccgtcg gacggaatga attccgagaa cgcgtgacaa gattattttt
    12121 aaaattttat cccccctacc ccccttcgaa caaggagggc cccgaataac gtccgcgtcc
    12181 tcgggaactg gaatcaggga agggccgcgg g
//
LOCUS       DAOOXH010000075        12109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_5495_length_12109_cov_7.375975, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000075 DAOOXH010000000
VERSION     DAOOXH010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 12109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12109
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5495_length_12109_cov_7.375975"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PKV64_08770"
     CDS             complement(<1..233)
                     /locus_tag="PKV64_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17207.1"
                     /translation="MKKALALALVLSVVGFSCSPGKTEGTKLAEGTPAYQLAKDLSAV
                     LPLFDPDKNTVLYEAKKFTVTAGDREFLGFIKNR"
     gene            331..2265
                     /gene="argS"
                     /locus_tag="PKV64_08775"
     CDS             331..2265
                     /gene="argS"
                     /locus_tag="PKV64_08775"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPN17208.1"
                     /translation="MLVLKEKLRTILYERLKDLYPIVREDIDFLYPPQAKFGDLALSL
                     PLSLAKKIGDNPRKIAQKMAAEIGVPPGISRLEIAGAGFINLFLDPDPFFAERLASAG
                     RGALRPEERKIIVEHTNINPNKAAHIGHLRNACLGDTLVRCLRAKGETVEVQNYLDDT
                     GVQVVDVVYGLMELERKTEADLDRIERFDYYCWDLYARTVAYLEEHPESRGRKAEILK
                     NIEHGRPPESGMAFAVSRRVARAHLRTMTRIGAAYDVLPCESSILGLRFWERAFALLK
                     ERRAISLQTEGPNAGCWVMKVEDEDDREKIIVRGDGTVTYVGKDIAYQMWKFGLLGRD
                     FYYEPFAEENGRTLWISTAEPNGRKVSFGGAGRVYNVIDSRQAYLQKVVVQGLRALHF
                     EEQAERSVHFSYEMVALSPRSLKYLGLEAAAGEEGKSFVEVSGRKGLGVKADDLLDLL
                     EEKARAEVDKRNPDLPDEAKNRTAADIAKAALRYFMLKFARHSMIVFDVDEALSFEGE
                     TGPYLLYTVVRLNSIFRKLEEREGTSREDIERRASAPPPSFDVLDAAERTELWDLIAA
                     SSKLEEEILHSLASLEFSHLAKFAFLLSQKVNAYYHRRPVLSEERPDVRGLRILGLHH
                     VRGVLERVLDLMGLSVPERM"
     gene            2564..4801
                     /locus_tag="PKV64_08780"
     CDS             2564..4801
                     /locus_tag="PKV64_08780"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HPN17209.1"
                     /translation="MFKGKTLLLVEDEALVARDEAKQLQDEGYQVIVVHGGEEAIEAV
                     RIRGKEIDLILMDINLGRGMDGTETARRILKDRDIPIVFLSSHTEKHFVDKTEDIASY
                     GYVIKDAGITVLQASVGMAFRLHEAHRKRRSAEEKLRASELFNFALFQFNPIQTVIVD
                     REGRIVKTNLAKKQSGDRLPQIGDRMYTDYAARHGIDMRGALTAAIASGKPVHFFALP
                     YEGKYLDISIAPFSEGAIITSLDVTERVQAAEKARLSEQRLALAQKAAKAGVWNWDAA
                     SGRLILSPELYAIIEKTPKESEDPVDLWKTLIHPEDRKSARLRLERALTGRIPFEDEV
                     RILRPGGQTRWLLVMGSAAHDDSGDAVRMDGICLDITEQKSREDIRTAVYEIAQVALR
                     AENLGEIFKALHLIVSRLIPAENFYIALYDSEKKLLSFPYFIDEYDEPPLPQPIGRGL
                     TEYVLFHGEPLHATPQKAEELEKSGEVVLIGAPSADWLGVPLKIRGQTIGVMVVQTYV
                     AGALYKNEDMDMLNFVSGQAALSIERKRSEQELQQLVKQKELLMRELQHRTKNSLALI
                     SSLLGLEKENLADARAREIFTKTRARIGSIATVYERLSDSANYDRVNLHVYVQEIAEA
                     VIRSFAPEANNVRLKTKLEEIKLDPKRSVPVGLILTELVMNALKYGLPGKGIGEIRLE
                     LKETDGRIFLEVADNGGGLPPGFDYATVSGTGLTIVRMLVEQLDAELTFTGRRGTRVQ
                     LSFNR"
     gene            4798..5940
                     /gene="mutY"
                     /locus_tag="PKV64_08785"
     CDS             4798..5940
                     /gene="mutY"
                     /locus_tag="PKV64_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPN17210.1"
                     /translation="MIRRRRAIRRSDLPAESNGRCDEGWDALFPGRLLRWYAARKRDL
                     PWRRTRDPYRVWVSEIMLQQTTVGAVIPYYNAWLRAFPDLQTLARAPLQKVLKAWEGL
                     GYYQRARNMRRTARIIRDRCGGRFPEDPADLAALPGFGPYTAAAVASLAFHRPVPVVE
                     ANVRRVMTRMLGLAGPPGAGRDAAIARFLEKRISRRSPGNFNQAMMEWGALVCRPKNP
                     LCLKCPGQNFCRAFARGEQDVLPAPKTLRPKDITAVVAVIRKNGRVLLQKRPETGLLA
                     GLWEFPGGKVEAGETLRAALRRELREERGFAVESARPLMTVRHSYTRFRVTLHAFDVT
                     IAGPGGPRPPDFRGDSSVRWVSLGALDRYPFPSGSLKIVRHLQSRG"
     gene            complement(6090..6410)
                     /locus_tag="PKV64_08790"
     CDS             complement(6090..6410)
                     /locus_tag="PKV64_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HPN17211.1"
                     /translation="MIITTTPAVEGRKISSYLGIVTGEAILGANMFKDLFAGIRDLVG
                     GRSAAYERELQNARDIALAEMSDRAVRLGANAVVGVDLDYEVLGQTNGMLMVSVSGTA
                     VRLD"
     gene            6512..7543
                     /locus_tag="PKV64_08795"
     CDS             6512..7543
                     /locus_tag="PKV64_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17212.1"
                     /translation="MVARFDFVGFGTRCASEPGRLVLDVGMTMAPGVLDHHFAEAESE
                     CAASLVVKHPELVLDHLRPGGRPADALGPVTIVTHTLPDFDALAAVFLALRLLETGVV
                     DEGMRTLAEYAKLADSALLPATIDLGATPYAVLRALFVGVRKAPEEINAGRISEGLKF
                     MRHLHARASEGGDITEDRTLFAGIDRYEQAVRKVDQDNAWYQGDVHHARKITLHLPNI
                     SGAGRTAVDGLIVVNPHSFLFKEWARRDRGGSPLGRGFSFVFQNFLGLRYSLAVDPAA
                     GVNLRDLAEVINSLESRAAEAAGTDDRLRWYGGECPFFNHRLVVSPHEGTRLSHDEIA
                     AAVLRFGEA"
     gene            complement(7570..8748)
                     /locus_tag="PKV64_08800"
     CDS             complement(7570..8748)
                     /locus_tag="PKV64_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPN17213.1"
                     /translation="MKPSQIVEGLWSVGAVDWDRRLFDSLIPLPDGTSYNSYLVRGTE
                     KTALFDAVDPSRADILLENLKDVPSLDYVVAHHAEQDHSGSLPAVLEKYPAAKLVCSP
                     KAKGILVDHLGLPEERIVVAADGETLSLGGKTLEFVHTPWVHWPETMCTHLREDGVLL
                     SCDFFGSHFAQSGLFVEDERAIYPAAKRYFAEIMMPFRAAIRKNMDRLKALDIRIIAP
                     SHGPVYGRPAFILDAYRDWISETPKNSVLIAHVSMHGSTERMVARLISALAERGVDVQ
                     AFNVSAVDLGKMAAALIDAATIVLASPAVHVGLHPNAASAAFLVNALRPKARFAALVG
                     SYGWGHKMAEQAVGLLPNLKVEVLPPLFVKGMPREADLRAVDELAETIARKHREAGLA
                     "
     gene            complement(8800..10212)
                     /locus_tag="PKV64_08805"
     CDS             complement(8800..10212)
                     /locus_tag="PKV64_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN17214.1"
                     /translation="MPHDIPPGERRIVLVLASVAAFLTPFMGASLNVAVPVIGREFRM
                     SAVSLGWIAMTYLLAAAVCLVPFGKIADLTGRKRIFGWGSLLHAASSLLAALAGSTAV
                     FFGARVLQGIAGAMLISTGVAMLTSVYPPGERGRVLGLNVSFTYLGLSLGPVLGGAMT
                     QRWGWRSIFLAASALGFILALIVIGTLKGEEWTGGEKQAVDAAGALLMAGGLISLMLG
                     FSRLPEPEGFLLLAAAAAFLVLFILREKRAPAPILDLNLFRGNRVFAFSNLAALINYS
                     ATTAVGFVLSLHLQIVKGLSPQAAGLVLLAQPAMMVLFSPLAGRLSDRIEPRFLASSG
                     MGIIAAGLLLLAGLNGATSTAVIAGVLLVLGFGFALFSSPNTNAVMGAVDKRSYGVAS
                     ALLGTMRITGQMTSMGLATLVFALRIGRVAISAEAHPGFLAASRIIFLICAALCLAGV
                     FASLARGKMHETAGSLTSVD"
     gene            complement(10223..10849)
                     /locus_tag="PKV64_08810"
     CDS             complement(10223..10849)
                     /locus_tag="PKV64_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17215.1"
                     /translation="MASVLLYLGGAIFVPGRAFRRLAKERRRFAKGFRVVFLVGILYT
                     LSAAGLAVSGALIPAPVFLPLDAHNYYFFEIFFALPAFLLAWLAASAAGTLVSLVFRG
                     RGGFKAAAAPWAFAFAGPSFLMWLPHAVFSGFLLLGMSQQEFMSYTADPGFLQTAFLI
                     YQGLAALLLAAGSVRAAAAGRSLRPVGALLTGLFAAAVFGGIIGLVIR"
     gene            complement(10860..>12109)
                     /locus_tag="PKV64_08815"
     CDS             complement(10860..>12109)
                     /locus_tag="PKV64_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HPN17216.1"
                     /translation="TYDVDALKLIDDLSEYGLAVGGVVITRFDNQPAAKIFRSKLERR
                     GVRVYTHGYTRGYPTDVDLIVSDAGYGANEYIETNKPLVVVTGPGPNSGKLATCLSQI
                     YHDYRRGWKAGYAKFETFPIWNLPLKHPVNVAYEAATADIKDFNQVDPFHLEAYGIPA
                     VNYNRDIDVFPVVRRILEKITGGEPLYRSPTEMGVNRAGFGIIDDAVCRNAALQELIR
                     RYFRYACEYALGFVEKDTVERAELIMREMNVKPEDRRVVEPARAAGESAAARGKGHKG
                     VYVGSAMELPNGTIVTGVNSPLLHAASSLLLNAGKTMAGIPDPIPLLSASTLEAVARL
                     KEDILGMNSASLNVDEVLICLSLGAAANPSAQLALDKLKSLRGSEVHMTHMPTPGDEA
                     GLRRLGFHVTADPNFSSKNLFII"
BASE COUNT         2296 a         3914 c         3834 g         2065 t
ORIGIN      
        1 cggttcttta tgaagccaag aaattcacgg tcgccggcgg tgaccgtgaa tttcttggct
       61 tcataaagaa ccgtgttctt gtccgggtcg aacaacggca gaacggccga caggtccttg
      121 gccagctgat aggccggcgt gccctcggcg agcttcgttc cctcggtctt gccgggggaa
      181 caggaaaatc caacgacgct taggaccaac gccaaagcga gtgctttttt catggtgtct
      241 ccttgtccgt gaaaaaacag tagagcatat tcagccggtt ttgccaagag cctgaacagg
      301 ggaaaagccc tgtgctataa tggggcggtt atgcttgtcc tgaaagaaaa acttcggacc
      361 attctctacg aacggctgaa ggacctctac cccatcgtcc gggaagacat cgattttctc
      421 tacccgcccc aggccaaatt cggcgacctg gccctctcgc ttcccctctc cctggccaaa
      481 aaaatcgggg acaatccccg gaaaatcgcc caaaaaatgg cggccgagat cggcgttccg
      541 ccggggattt cgcgcctgga aatcgccggg gccggcttta tcaacctgtt tttggatccg
      601 gatccgtttt tcgccgaacg gctcgcttcg gccggacggg gcgccctgcg gccggaggaa
      661 cgaaagatca tcgtcgagca tacgaacatc aaccccaaca aggcggccca catcggccat
      721 ctccggaacg cctgcctggg cgacaccctc gtccggtgcc tccgggccaa gggggaaacc
      781 gtcgaggtcc agaattatct cgacgacacc ggcgtccagg tcgtcgacgt cgtttacggc
      841 ttgatggagc tcgagcggaa gacggaggcc gacctggacc ggatcgagcg gttcgattat
      901 tactgctggg atctctacgc ccggaccgtc gcgtacctgg aggagcaccc cgaaagccgg
      961 ggccgcaagg ccgagatcct caagaacatc gaacacggcc gcccgcccga gagcgggatg
     1021 gccttcgccg tttcccggcg cgtcgcccgg gcccatctcc ggacgatgac gcggatcggc
     1081 gcggcctacg acgtccttcc ctgcgagtcg agcattctcg gcctccggtt ctgggagcgg
     1141 gccttcgccc ttctcaagga gcgccgggcg atctcgcttc agaccgaggg accgaacgcg
     1201 ggctgctggg tcatgaaagt cgaggacgag gacgaccggg aaaaaatcat cgtccgcggc
     1261 gacgggacgg tcacctacgt cggcaaggac atcgcttacc agatgtggaa attcggcctc
     1321 ctcgggcggg atttctatta cgaaccgttc gccgaggaga acggccggac cctctggatc
     1381 tcgacggccg aacccaacgg ccggaaggtc tcgttcggcg gggccggccg cgtttacaac
     1441 gtcatcgatt cccgccaggc ctatctccag aaagtcgtcg tccaggggct ccgggcgctt
     1501 cacttcgagg agcaggccga gcggtcggtc catttctcct acgagatggt cgccttgtcc
     1561 ccccggagcc tcaaatacct ggggttggaa gcggcggccg gcgaggaggg gaaaagcttc
     1621 gtcgaggtct ccgggcggaa aggcctcggg gtcaaagccg acgatctcct cgacctcctc
     1681 gaagagaaag cccgggccga agtggataag cgcaatcccg acctgccgga cgaggctaag
     1741 aaccggacgg cggccgacat cgccaaagcg gccctgcggt atttcatgct gaagttcgcc
     1801 cgccattcga tgatcgtctt cgatgtcgac gaggccctca gcttcgaggg cgagaccggc
     1861 ccttatctcc tctacacggt cgtccgcctc aacagcatct tcagaaagct ggaggagcgc
     1921 gaggggacga gccgggaaga catcgagagg cgggcgtccg cccccccccc ttccttcgac
     1981 gtcctggacg cggccgaacg gaccgagctc tgggatctga tcgccgcctc ctccaagctc
     2041 gaggaggaga tcctgcattc cctggcctcg ctcgagttct ctcacctggc caagttcgcc
     2101 ttccttctct cccaaaaagt caacgcctat taccaccgcc ggccggtcct gtccgaggaa
     2161 cggccggacg tcaggggcct gcggattctc gggctccatc acgtccgcgg cgttctggag
     2221 cgggttctcg atctcatggg cctctcggtc ccggaacgga tgtgacccgt cgcactcaga
     2281 aggattccaa gaattcgaga catccccgcc ccgcggccta agccgccttt ccgcggacgg
     2341 agatgaacgc tccccgcggc gcgccttcct gaagcgggcg gccttcggct cgtttttcga
     2401 ttcctctctt caaagaaaag ggccgccgtc gtttcgcctc gggaatcatg ccgggggaat
     2461 ccgttcttga ctcgcaccgg cgggggggct atatttcgat cattatgagc aagacacggc
     2521 tcgtttttct ccggcccggc gaactccggc cggcggcttg atcatgttca agggcaagac
     2581 cctcctcctc gtcgaagacg aagccctcgt cgcccgggac gaggccaagc agctccagga
     2641 cgaaggatac caggtcatcg tcgtccatgg cggagaagag gcgatcgagg ccgtccggat
     2701 ccggggaaaa gaaatcgatc tcattctgat ggacatcaat ctcggccgcg gaatggacgg
     2761 gaccgagacg gcccgtcgga tcctcaagga ccgcgatatt cccatcgttt tcctctcgtc
     2821 ccacacggaa aaacatttcg tcgacaaaac ggaagacatc gcctcgtacg gctatgtgat
     2881 caaggacgcc gggatcacgg ttcttcaagc ctcggtcggc atggccttcc ggctccatga
     2941 ggctcaccgg aagcgccgct cggcggagga gaagctccga gccagcgaac tgtttaattt
     3001 cgccctcttt caattcaatc cgatccaaac cgtgatcgtc gaccgggaag gccggatcgt
     3061 caaaaccaac ctggccaaaa aacagtccgg ggaccgtctt ccccaaatcg gcgaccggat
     3121 gtacacggac tatgcggccc ggcacgggat tgatatgcgg ggcgccttga cggccgccat
     3181 cgcttccggg aaacccgttc atttctttgc cctgccctat gaagggaaat acctcgacat
     3241 ctccatcgcc cccttctccg agggcgcgat catcacgagc ttggacgtca ccgagcgggt
     3301 tcaagccgcc gaaaaagccc gtctcagcga acagcggctg gccttggccc agaaggcggc
     3361 caaggccggc gtgtggaact gggacgccgc ctcgggccgc ctgatcctgt cgccggagct
     3421 ttacgcgatc atcgaaaaaa caccgaagga atcggaagat ccggtcgacc tctggaaaac
     3481 cctcatccat cccgaggacc ggaaaagcgc ccgtctccgc ctggaaagag ccttgaccgg
     3541 ccgcattccc ttcgaggatg aagtccgcat cctccggccc gggggacaaa cgcgctggct
     3601 cctggtgatg ggaagcgcgg cccatgatga ttccggcgac gccgtccgga tggacgggat
     3661 ctgcctcgac atcaccgaac agaaatcccg ggaagatatc cggacggccg tctacgagat
     3721 cgcccaggtc gccttgcggg cggaaaacct cggggaaatc ttcaaagccc tccacctgat
     3781 cgtcagccgg ctgatcccgg cggaaaactt ctatatcgcc ctttacgatt ccgagaaaaa
     3841 gctccttagc ttcccttatt tcatcgatga atacgacgaa ccgccgcttc cccagccgat
     3901 cggccggggt ttgaccgagt acgttctgtt ccacggcgaa ccccttcacg ccaccccgca
     3961 gaaggccgag gagctggaaa aatcagggga ggtcgtgctc atcggcgccc cctcggccga
     4021 ttggctgggc gtcccgctga agatccgggg ccagacgatc ggcgtcatgg tcgtccaaac
     4081 gtacgtggcc ggggcgctct acaagaacga agacatggac atgctgaatt tcgtctccgg
     4141 tcaggccgct ctctccatcg agcggaaacg gtcggaacag gagctccagc agctcgttaa
     4201 gcaaaaagag ctcctgatgc gcgagctgca gcaccggacg aagaacagcc tggccctcat
     4261 ctccagcctt ctcgggctgg agaaggagaa cttggccgac gcccgggccc gggaaatctt
     4321 caccaagacg cgggcccgga tcggctcgat cgccaccgtc tatgagcggc tgtcggattc
     4381 ggccaattat gaccgggtga atctgcacgt ctatgtccag gagatcgccg aagcggtcat
     4441 ccggtccttc gccccggaag ccaacaatgt ccggctgaag accaagctcg aggaaatcaa
     4501 gctcgacccg aaacgatccg tccccgtcgg cttgatcctg accgagctgg tcatgaacgc
     4561 cctcaagtac ggcctccccg gaaaaggcat cggggaaatc cggctcgagc tgaaggagac
     4621 ggacggccgg atcttcctcg aggtcgcgga caacggcggc ggcctccccc ccggctttga
     4681 ttacgccacg gtttccggga cggggctgac catcgtccgg atgctggtcg aacagctcga
     4741 cgcggagttg acgttcaccg gccgccgcgg cacgcgcgtt cagctgagct tcaaccgttg
     4801 atccggcgga gaagagccat tcgccggtca gatctccccg cggaatcgaa cgggagatgc
     4861 gacgaaggct gggacgccct tttccccggc cggcttctcc gttggtacgc cgcccgcaag
     4921 cgcgaccttc cctggcgccg gacgcgcgat ccctatcgcg tctgggtgtc ggaaatcatg
     4981 ctccagcaaa cgacggtcgg agccgtcatc ccctattata acgcctggct gagagccttt
     5041 cccgatctcc aaacgctcgc ccgggcgccg ctccagaagg tgctcaaagc ctgggaaggc
     5101 ctgggctatt atcagcgcgc ccggaacatg cgccggaccg cccggatcat ccgggatcgt
     5161 tgcggcgggc ggtttcccga ggatccggcg gacttggccg ccctccccgg tttcggcccc
     5221 tacacggcgg cggccgtggc cagcctggct ttccatcggc cggtccccgt cgtcgaagcc
     5281 aacgtccgac gggtcatgac gcggatgctc ggcctcgccg ggccgccggg agccggccga
     5341 gacgcggcca tcgcccgctt cctggaaaag cgcatctccc gccggtcgcc cggcaatttc
     5401 aaccaggcga tgatggaatg gggagctctc gtctgccgcc cgaaaaatcc gctttgtctt
     5461 aagtgccccg ggcaaaattt ctgccgggcc ttcgcccggg gggaacagga cgtccttccc
     5521 gccccgaaga ccctccggcc gaaagacatc acggccgtcg tcgccgtcat ccgcaaaaac
     5581 ggccgcgttc tcctccagaa acgtcccgag acggggcttc tggccgggct ttgggagttt
     5641 cccggcggga aggtcgaggc gggcgaaacg ctccgggcgg ccctccgccg ggagcttcgg
     5701 gaggagcggg ggttcgccgt tgaatccgcc cggcctttga tgaccgtccg gcattcctat
     5761 acccggttcc gggtcaccct ccacgcgttt gacgtcacga tcgccggccc cggcggcccg
     5821 cgtccgccgg atttccgggg cgattcctcc gtccgctggg tttctttggg cgccttggac
     5881 cgttacccgt ttccctccgg ctccttgaaa atcgtccggc atcttcagag ccggggctga
     5941 gccggcgccc cgtccctata aattgaggga agcaacaccc tgcaatcaag atggcctctc
     6001 aacgggccga tgggccgttc tttcggccac ggcgcctttc ccgcttctcc cctggcccgg
     6061 accggcattc agggctccgg ccggctgttt cagtcgagcc ggacggcggt tccggaaacc
     6121 gaaaccatca gcatgccgtt ggtttgcccg agaacctcgt agtccaagtc gacgccgacc
     6181 accgcgttgg ccccgagacg gacggcccgg tcgctcattt cagccagggc gatgtcccgg
     6241 gcgttctgaa gctcgcgctc gtaggcggcc gaccggccgc cgaccaagtc ccggataccg
     6301 gcgaacagat ccttgaacat gttggctccg agaatggcct cgccggtgac gatgcccagg
     6361 taggaggaaa ttttccggcc ttccaccgcc ggggtggtcg tgatgatcat cgcggatctc
     6421 ctttcgttgt cgctgatcgt gtcttcaacg tctttccata cggaaaccgc ggcggtttgt
     6481 ttatcccccg aggtgtgcta tcttcacgag catggtggcg cgcttcgatt tcgtcggctt
     6541 cgggacgcgg tgcgcgtccg agccgggacg gctcgtcctc gacgtcggga tgaccatggc
     6601 cccgggcgtg ctggatcatc atttcgccga ggcggaaagc gagtgcgcgg cttcgctggt
     6661 ggtcaagcat ccggagctcg tcctggatca cttgcgcccc ggcggccggc cggccgacgc
     6721 cctcggcccg gtgacgatcg tcacccatac cctgcctgat ttcgacgcct tggccgcggt
     6781 ctttctcgcc ctccggcttc tcgaaaccgg cgtcgtggac gagggcatgc ggacgctggc
     6841 ggagtatgcc aagctcgccg actcggccct gctgccggcg acgatcgacc tcggggccac
     6901 cccctacgcc gtccttcggg ccctttttgt cggggtgaga aaagcgccgg aggaaatcaa
     6961 cgccggccgg atctccgagg gtttgaaatt catgcgccac ctccacgccc gggcatccga
     7021 aggcggggat atcaccgagg accggacctt gttcgccggc atcgatcgct acgagcaggc
     7081 cgtccgaaaa gtcgaccagg acaacgcctg gtaccagggg gatgtccacc acgcccggaa
     7141 aatcacgctc catctcccga acatctcagg ggccgggcgg acggccgtcg acggactgat
     7201 cgtcgtcaac ccccacagct tcctgttcaa ggaatgggcc cgccgggacc gcggcggctc
     7261 gccgctcggc cgcggcttca gcttcgtttt ccagaatttt ctgggattgc gctacagcct
     7321 ggccgtcgat ccggccgccg gcgtcaacct ccgcgacctc gccgaggtca tcaacagcct
     7381 ggaatcgcgg gcggccgaag cggccgggac ggacgaccgg ctccgctggt acggcggcga
     7441 atgccccttc ttcaatcacc ggctggtggt ctccccccac gaagggacgc gcttgtccca
     7501 cgacgagatc gcggccgcgg tcctgcgctt cggggaggcc tgagggcatt tccggagccg
     7561 gcccggccgt caggccagcc cggcctcccg gtgcttccgg gcgatggttt cagccagctc
     7621 gtcgacggcc cggaggtcgg cttcccgcgg cattcccttg acgaacagcg gcggcaggac
     7681 ttcgaccttc aggttcggca gcaggccgac ggcctgctcg gccatcttgt gcccccatcc
     7741 atacgatccg accagggcgg cgaaccgggc cttcggccga agagcgttga ccaggaacgc
     7801 ggctgaggcc gcgttcggat gcaagccgac gtggacggcc ggcgaagcca agacgatcgt
     7861 ggccgcgtcg atcagggcgg cggccatttt cccgagatcg accgcggaga cgttgaaggc
     7921 ctggacgtcc acgccgcgct ccgccagggc ggagatcagc cgggcgacca tccgttccgt
     7981 gctcccgtgc atggacacgt gggcgatcag gacgctgttt ttgggagtct ccgagatcca
     8041 gtcgcggtag gcgtcgagga tgaaggccgg ccggccatac accggtccgt ggctcggggc
     8101 gatgatccgg atgtccaggg ctttgagccg gtccatgttt ttccggatgg ccgcgcggaa
     8161 gggcatcatg atctcggcga aatagcgctt ggccgcggga taaatcgcgc gctcgtcctc
     8221 gacgaacagc ccggattggg cgaagtggga gccgaagaaa tcgcaggaca gaagcactcc
     8281 gtcttcccgg aggtgcgtgc acatcgtctc cggccagtga acccaaggag tatggacgaa
     8341 ttccagggtc ttgccgccca gggagagcgt ctcgccgtcg gccgcgacga cgatcctctc
     8401 ctccggaagg ccgagatgat ccaccaggat gcccttggct ttgggggaac agacgagctt
     8461 ggccgcggga tatttttcca ggacggcggg aagggagccg ctgtgatcct gttcggcgtg
     8521 atgggcgacc acataatcca gcgacgggac gtccttcaag ttctccagga gaatgtccgc
     8581 ccgggaggga tccacggcgt cgaagagggc ggttttctcc gtcccccgga cgagatagga
     8641 gttatagctt gttccgtcgg gcagcgggat cagggaatca aacagccgcc ggtcccaatc
     8701 caccgccccc acggaccaga ggccttcaac gatctgactc ggtttcataa tcatcctttc
     8761 cggcccgggg ccgattattt cctagtaatt ttataggaat taatccacgc ttgtcaacga
     8821 tcccgccgtc tcgtgcatct tcccccgggc cagcgaggcg aagactccgg ccaggcacag
     8881 ggccgcgcat atcaggaaga tgatccggga agcggccaag aaaccgggat gcgcctcggc
     8941 cgagatcgcg acccggccga tccgcagggc gaagacgagg gtggccaggc ccatgctggt
     9001 catctggccg gtgatccgca tcgtcccgag aagagccgag gccacgccgt aggagcgctt
     9061 gtccaccgcc cccatcaccg cgttggtgtt gggcgaggaa aagagggcga agccgaatcc
     9121 caggacgagg aggacgccgg cgatgacggc cgtggaagtc gctccgttca aaccggccag
     9181 caagagcaag ccggcggcga tgattcccat ccccgaggag gccaggaacc ggggctcgat
     9241 ccggtccgag aggcgcccgg ccaggggcga aaacaaaacc atcatcgccg gctgggccag
     9301 gagaacgagg ccggcggctt gcggggacaa gcccttgacg atttgaagat gcaggctcag
     9361 gacgaatccc acggccgtgg tcgcgctgta attgatcagg gcggccaggt tcgagaaagc
     9421 gaaaacgcgg tttccccgga aaagattcag atcgagaatc ggggccgggg cccgtttttc
     9481 cctcaggatg aaaagaacga ggaaagcggc ggccgccgca agaaggagga atccctcggg
     9541 ctcgggcagc cggctgaagc ccagcatgag gctgatcagt cctccggcca tcaaaagcgc
     9601 gccggcggcg tcgacggctt gcttttcccc gcccgtccat tcctcgccct tcagcgttcc
     9661 gatgacgatc aaggccagga tgaaacccag ggccgaggcg gcgaggaaga tgctcctcca
     9721 gccccagcgc tgggtcatcg ctcctcccag gaccgggccg agggaaagcc ccaggtacgt
     9781 gaaggaaacg ttgaggccca ggacgcgccc ccgctccccc ggcggataga ccgaggtcag
     9841 catggccacg ccggtgctga tcagcatcgc cccggcgatc ccttggagga cgcgggcgcc
     9901 gaaaaaaacc gccgtggatc cggccagggc ggccaaaagc gacgaggcgg cgtggagaag
     9961 gcttccccag ccgaaaatcc gcttccgccc ggtgaggtcg gcgattttcc cgaacggcac
    10021 gaggcagacg gccgcggcca ggagataggt catggcgatc cagccgaggg agacggccga
    10081 catgcggaac tcccggccga tgaccgggac ggcgacgttc agcgaggcgc ccatgaaagg
    10141 cgttaaaaac gccgcgacgg aagcaagaac caggacgatt cgtcgttccc ccggcggaat
    10201 atcgtgcggc atggcttctc tttcagcgga tgacgaggcc gatgatcccc ccgaaaacgg
    10261 ccgcggcgaa aagccccgtc aaaagggcac cgaccggccg gagggaccgt ccggcggcgg
    10321 ccgcccggac cgatccggcg gccagcagaa gagccgccag accttgataa atcaaaaacg
    10381 cggtctgcag gaatccggga tcagccgtgt acgacatgaa ctcctgctgg gacatcccca
    10441 gcagaaggaa tcccgaaaag accgcatgcg gcagccacat caggaacgac ggcccggcga
    10501 aggcgaaggc ccagggagcc gcggccgcct tgaatccccc ccgtccccgg aaaaccaggg
    10561 aaacgagcgt tccggcggcg gaggcggcca gccaagccag aaggaacgct ggaagggcga
    10621 aaaaaatctc aaaaaaatag taattgtggg cgtcaagcgg caggaacacc ggggcgggaa
    10681 tcagcgcccc ggaaacggcc aggccggccg ccgacagcgt gtagaggatg ccgaccagaa
    10741 agacgacgcg gaatcccttg gcaaacctcc gccgttcctt ggccagacgg cggaaggccc
    10801 ggccgggcac gaagatcgcg ccccccaggt aaagcagaac ggacgccatg aattctctct
    10861 caaatgataa acaggttctt gcttgaaaaa ttcgggtcgg cggtcacgtg gaaccccaaa
    10921 cggcggagtc ccgcctcgtc gcccggcgtc ggcatgtggg tcatatggac ttccgacccg
    10981 cggaggcttt tgagcttgtc cagggccaat tgggccgagg gattggccgc cgcccccagg
    11041 ctcaggcaga tcaagacttc gtccacgttc aggctggccg aattcatccc gaggatgtcc
    11101 tccttcagac gggcgacggc ctccaaggtg gacgccgaaa gcaggggaat cgggtcgggg
    11161 attccggcca tggtttttcc ggcgttcagg aggaggctcg aggcggcgtg gagaagaggc
    11221 gaattcaccc cggtgacgat cgtcccgttc ggcagctcca tggccgagcc gacatacacg
    11281 cccttgtgtc ccttcccccg ggcggcggct gattcacccg cggcccgggc cggctccacg
    11341 acccggcggt cctcgggctt gacgttcatt tcccgcatga tcagctcggc cctctcgacg
    11401 gtgtcctttt cgacgaatcc cagggcgtac tcgcaggcat agcggaaata gcggcggatg
    11461 agctcctgga gggcggcgtt ccggcagacg gcatcgtcga tgatgccgaa tccggcccgg
    11521 ttgacgccca tctccgtggg cgaccggtac aggggctctc cgccggtgat tttttccagg
    11581 atgcgccgga ccacgggaaa gacgtcgata tcgcggttgt aattgaccgc ggggattcca
    11641 taggcctcga gatggaaggg gtcgacttgg ttgaaatcct tgatgtcggc cgtggccgcc
    11701 tcgtaggcga cgttgaccgg gtgcttgagg ggcaggttcc agatcggaaa ggtttcgaac
    11761 ttggcgtagc cggctttcca tccccggcgg taatcatggt agatctggga aaggcaggtc
    11821 gccagctttc cgctgttggg gcccggaccg gtgacgacga cgagcggctt gttcgtctcg
    11881 atatattcat tggccccgta cccggcgtcg ctgacgatga ggtcgacgtc ggtcggatag
    11941 ccgcgggtgt agccgtgggt gtagacccgg acgccccggc gctctagctt gctccggaag
    12001 attttggccg cgggctggtt gtcgaagcgg gtgatgacga cgccgccgac ggccaggccg
    12061 tattcggaga gatcatcgat aagcttcaag gcgtcgacgt cgtaggtga
//
LOCUS       DAOOXH010000076        11990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_5587_length_11990_cov_8.492250, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000076 DAOOXH010000000
VERSION     DAOOXH010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 11990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11990
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5587_length_11990_cov_8.492250"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PKV64_08820"
     CDS             <1..487
                     /locus_tag="PKV64_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPN17217.1"
                     /translation="PPIRIIIPGKVYRRETPDPTHAAMFFQVEGLVVDRGITFAHLKG
                     TLEHFLKRLFGDKLKTRFRPSFFPFTEPSAEVDIECLACGQKDPACRVCNGTGWKEIL
                     GSGMVDPQVFKNVNIDPEQYSGWAFGMGVERIAMFAYGIPDIRHFYENDLRFNRQFQL
                     K"
     gene            484..2541
                     /gene="pheT"
                     /locus_tag="PKV64_08825"
     CDS             484..2541
                     /gene="pheT"
                     /locus_tag="PKV64_08825"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPN17218.1"
                     /translation="MKISLRWLQEYVDIEMPRSELLNRLTMIGLICEEWTETTDGDTV
                     LDIETYANRPDTLGHLGIAREISTMTGSPLKERLHPVVELPAATSELVEVEVLDEDLC
                     SRYTGFVVKGLSVGPSPDSLRKKIEAMGAKPINNIVDASNIVLFATGHPVHMFDLNKV
                     AGPRVVVRRAKKGETLRTLDGRERAMAPDMLVIADDRRPIAVAGIIGGEESGITSATR
                     DIFIESAVFDPASIRRTRKALEIVTDASYRFERGADIGFAPEAAAMAASLLCSFGGRV
                     SKGLVDLYRRPRKPREVVLRSARTAELLGVAVPDAFIEKTLADLGFSTKSKAPGSWLV
                     TVPSFRVDIEREADLIEEIARFFGYDRIPSVVPPLDALEPIPSNRDRIDRLSRRLFHY
                     GFDEVVNASFADPEKEAVLQTERTPVTLRNPFSVHAAALRTTLLGGLLINLRHNQNHG
                     TEDVHIFEIGRIFHRIEDAPPAEELFLGLLSAGPVGLPHWSRPPSAANFPHLKGALES
                     ALAALRYETLVYEPAEHPAFAEGSALAVLYKGEKIGLLGRVRESVCEAAGTTGPVFAA
                     EIDLGCLLEKKPAAFAYEPPPKVPAVVRDLSFFVSRDIFYQEIKSAVERAAVPYLESF
                     DIIDRYAGPHHPDGRTSLSMRFVFRNPQATLLAEEADKSVQKVLKVLKSAFQIELRKG
                     DGA"
     gene            2538..2798
                     /gene="zapB"
                     /locus_tag="PKV64_08830"
     CDS             2538..2798
                     /gene="zapB"
                     /locus_tag="PKV64_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HPN17219.1"
                     /translation="MTNEIDRLKILEDKITGVIDHIQRLSTENEKLKQLVKDLKAEKR
                     DSEDLARKVVKLDGELKKYENEREEMKGKIEAIISQIDKLGM"
     gene            2798..3097
                     /locus_tag="PKV64_08835"
     CDS             2798..3097
                     /locus_tag="PKV64_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPN17220.1"
                     /translation="MSDIVEIEIYGRTYKIRVKGEENEEYISRLTSYVDQKMQEIAVK
                     SRSEDPTKIAVLAALNIADDYFVSRKRLEQVDERIDRMEAEIATVASNLFKDDHA"
     gene            3226..4791
                     /gene="rny"
                     /locus_tag="PKV64_08840"
     CDS             3226..4791
                     /gene="rny"
                     /locus_tag="PKV64_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPN17221.1"
                     /translation="MNIGILYIGLAVLIILAAGILFIQVKRTSGAKKLFRAGEEAAGL
                     VGQAKSEAQRLVSQAQNQARQKLASADAEFERMTQDKRRRLNEVERRLVHKEENLDRK
                     IQGLDRRDRDIATKERSLLQKEQEIENEAKRISSVIQDQLVELERISSMTQEEAKAQI
                     IRKVEDEARLEAMQTLKRLDEETRAKSRQLAAEVISGAIQRSAAEHVVESTVSVVDLP
                     SEDMKGRIIGREGRNIRALEVATGVDIIVDDTPEAVILSSFDPVRREAARLAIQRLVL
                     DGRIHPARIEEVVENVKAELEDKIRQDGEAAAMELRIQNLHPELIKLMGKLKFRTSYG
                     QNVLQHSKEVAFLSAAMAQELGLNTQTALRAGFLHDVGKAIDREVEGTHTELSVELCR
                     KYGENEAVVAAIASHHRDVDFPSIEAVLVQAADTLSAARPGARRELLENYIKRLEKLE
                     TLAKGFEGVSKAFALQAGREIRIMVDAGKVADERAGLIAKDIAAKIKNELDYPGQIKV
                     TVIREMRAVEYAR"
     gene            4781..5614
                     /locus_tag="PKV64_08845"
     CDS             4781..5614
                     /locus_tag="PKV64_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPN17222.1"
                     /translation="MPDKSGKEKSGTAVLRPSFRVLIIGDVIGRSGREALRRHLPVLR
                     DRHRPAFVVANGENAAGGVGLTEETGRELFSMVDVLTSGNHIWDKKEGIAYMEKESRV
                     LRPANYPPPAPGRGSVAVAGPDGLKLGVLNLQGRVFMEPIDDPFRAADAAVEELRKET
                     PCLIVDFHAEATSEKVALGYYLDGRVSAVVGTHTHVPTADGRLLPGGTAYVTDLGMAG
                     SRNSVIGIRKEQAILKFLTSRPIRFEPDKGGSFLSGVVVEIETATGRALSIVQEIIAE
                     D"
     gene            5690..7159
                     /gene="xseA"
                     /locus_tag="PKV64_08850"
     CDS             5690..7159
                     /gene="xseA"
                     /locus_tag="PKV64_08850"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPN17223.1"
                     /translation="MSGEKEGRGLPPEEAKSGRDLVREGSLVLRDHVYSVTEITQVIK
                     AALETAVPQVWVVGEISNCRRHPSGHIYFTLKDAGAALSAAVWKSNAARLKFELRDGQ
                     QVVCRGRIDVYPPRGSYQLNVDQVEPKGKGALQLAFEQLKEKLRAEGLFDPARKKKIP
                     LLPKTIGVVTSPTGAALIDILRTIDRRFAKVRIVIYPARVQGAGAAAEIVEGLEFLGG
                     RPGIDVIIVGRGGGSIEDLWAFNEEPVARAIAASPVPVISAVGHEVDFTISDFVADIR
                     ASTPTAAAEMVVEKEETFAERIDNLRRRAGQAARFGLQTRRGGVDELARHGVFEGFQR
                     RLWMLGQRVDELEGRAFDVVNAERRRIADGKARAVLAAGRMAAVLGRRLGQASGRAAL
                     VEERIGGAFRALLAKRMSEWERLSASLQALSPLDVLKKGYAVVWKEGTPRIPVTRAES
                     LRPGDAVAVSFHRGEFIADVRTVDPVRPVTVPDERERSS"
     gene            7156..7506
                     /gene="xseB"
                     /locus_tag="PKV64_08855"
     CDS             7156..7506
                     /gene="xseB"
                     /locus_tag="PKV64_08855"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017902317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPN17224.1"
                     /translation="MKEKDKTKKPDLDFEKALADLEGVVEELEKGGLSLNESLARFER
                     GVRLSRFLRNELDKAEKKIEILLKDEAGEVHPEPFSLEEAESGKPPVAKPGPASRKIP
                     PKTGTEDDDSELPF"
     gene            7702..9627
                     /gene="dxs"
                     /locus_tag="PKV64_08860"
     CDS             7702..9627
                     /gene="dxs"
                     /locus_tag="PKV64_08860"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPN17225.1"
                     /translation="MKRILDSIDSPADLKTLSLADMSRLAVEIRAEILDVVSRNGGHL
                     SSNLGVVELTLALHRVFDAPRDLIVWDVGHQTYTHKIITGRRDRFGTLRREGGLLGFP
                     SREESPYDPYNTGHASTALSAALGLAVARDMAGENRLVIAVVGDGSLTGGVSWEALNQ
                     IGNLRIPMIIILNDNEMSISQSVGAISKYLGYLASGQHYLRVKDKAKTLLKSVPGVGG
                     AIIRAGRGLEELVKKTFFPGLVFEELGLRYVGPVQGHSLPSLLEVFERAKKEAEGPVL
                     IHCVTRKGRGYTPAQNSPEHFHGAPPFDVESGEPAFSGTHPSYSEVFGETLLRIAGED
                     ERVVAITAAMREGTGLMEFAARRPAQFFDVGIAEQHAVNFAAGLAAGGRRPVCAIYST
                     FLQRAYDQIYHDVCLQKLPVVFVLDRAGLVPDDGPTHQGVNDIAYLRHMPNMVLLAPR
                     DENEMRHMLKAAFGYDRPVALRFPKGEITGAVPEPGWKDIPLGKAEILKDGENLVLAY
                     GSMVLPALEAAERAEKESAAAGAGLSLSVADARFCAPLDEELILRFARSGRAIATVEE
                     GVLAGGFGSAVLELINRRGLYGVPIRCFGLPVETFPVGKLGQLRRRYGLDADGLAAGF
                     RSFFSRLAKTEDGGPTS"
     gene            9663..10508
                     /locus_tag="PKV64_08865"
     CDS             9663..10508
                     /locus_tag="PKV64_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HPN17226.1"
                     /translation="MKKARADALLVERGLADSVAKARALIMAGRVASAGRQIEKAGEP
                     LAGDVPLSLKAGRPYVGRGGLKLAEALDAFRVSPSGLVCADIGVSTGGFTDCLLQRGA
                     LRVYAVDVSVRQIDDRLRRDRRVALIEKNARLLQPGDFPEPVGLVVMDVSFISILKIL
                     PALAAVLRSGRETAGGEEPILLSLIKPQFEADRRSVGRGGVIRNPEIHDRVLRRVAAG
                     ARPAGFFLSGLIRLNTRGQKGNVEFFGRFGLSAPAVEPLPGRVLEWISAAVKEADPDP
                     GGNEG"
     gene            10505..11350
                     /locus_tag="PKV64_08870"
     CDS             10505..11350
                     /locus_tag="PKV64_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPN17227.1"
                     /translation="MKTVRRAGIVIKPHAPGVAEVIAGLTAALEARGITPVLERAAAE
                     KVGRADGLARESIPAACDLVVVLGGDGTFLSVAHSAARADVPVMGVNLGSLGFLTEIP
                     LAEMTLALDAVLSGRTDIISPRMMLSTTFREKTELTLNDIVFNKAAKARMIHYVLSVN
                     GLEIAALRADGLVVATPTGSTAYSLSAGGPILQPSVPAILLTPICPHTLSFRPTVVSS
                     DSVIRIEVRTPGEEVYLTLDGQRGGEMDSEGIVEIRKADHVLRLVTSPQRRYFGLLQE
                     KLRWG"
     gene            11418..>11990
                     /locus_tag="PKV64_08875"
     CDS             11418..>11990
                     /locus_tag="PKV64_08875"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPN17228.1"
                     /translation="MMNPLLRRKPSVVLVSAEAFPFAGTRASAAGMQALPESLARLGF
                     DVCLVTPKYRRPAIEALPLTPVLRRFLVPVGKDKVPASAFRAETAISPLPPVDFSAVP
                     DPPARRFRAYFIDNVKYFDRERIFGSEKAPYLDNDERFAFFCRAVVEFLRRARIRIDI
                     LHCHDWPSALIPLFLRTAYDGQRPFRKTASV"
BASE COUNT         2231 a         3827 c         3842 g         2090 t
ORIGIN      
        1 gccgccgatc cgaatcatca tcccggggaa agtctaccgc cgggagacgc ccgacccgac
       61 ccatgcggcg atgttcttcc aggtcgaggg cttggtcgtc gaccggggga tcaccttcgc
      121 ccatctcaag gggacgctcg agcatttcct caagcggctg ttcggcgata agctgaagac
      181 gcgcttccgg ccgagcttct ttcccttcac cgagccgtcg gccgaggtgg acatcgaatg
      241 cctggcttgc gggcagaagg acccggcttg ccgggtctgc aacgggaccg gttggaagga
      301 aatcctcggc agcgggatgg tcgatcctca ggtgttcaag aacgtcaaca tcgatccaga
      361 gcaatactcg ggctgggctt tcggcatggg cgtcgagcgg atcgccatgt tcgcctacgg
      421 catccccgac atccggcatt tctacgagaa cgacctccgg ttcaaccggc aatttcaact
      481 caaatgaaaa tcagtctgcg ctggcttcag gaatacgtcg acatcgagat gccgcggagc
      541 gagcttctca accggttgac gatgatcggc ctcatctgcg aggaatggac ggaaacgacg
      601 gacggggata cggtcctcga catcgagacc tatgccaacc ggcccgacac cctcggccat
      661 ctcgggatcg cccgggagat ttcgaccatg acgggttccc ccctcaagga acgcctccat
      721 ccggtggtcg agctgccggc ggcgacgtcg gagcttgtcg aggtcgaggt tttggacgag
      781 gatctttgct cccggtacac gggattcgtg gtcaagggct tgtccgtcgg gccctcgccc
      841 gacagccttc ggaaaaagat cgaagccatg ggggccaagc cgatcaacaa catcgtcgac
      901 gcctccaata tcgtcctctt cgccaccggc catcccgtcc acatgttcga cttgaacaag
      961 gtcgccgggc cgcgcgtcgt ggtacggcgg gccaagaaag gggagactct ccggaccctg
     1021 gacggaaggg agagggccat ggccccggac atgctcgtca tcgccgacga ccggcgcccc
     1081 atcgccgtcg cagggatcat cggcggggag gaatccggaa tcacctccgc cacccgggac
     1141 atcttcattg aaagcgccgt cttcgacccg gcctcgatcc gccggacccg caaggccctg
     1201 gaaatcgtga ccgacgccag ctaccggttc gagcgcggcg cggacatcgg cttcgccccc
     1261 gaggcggcgg cgatggccgc ctcgctgctt tgttccttcg gcggacgcgt ctccaaggga
     1321 ctggtcgacc tttatcgccg gccgcggaag ccgcgggaag tcgtgctccg ctccgcccgg
     1381 acggccgagc ttctcggcgt cgccgtcccc gatgcgttca ttgagaaaac gctcgccgat
     1441 ctcggctttt cgaccaaatc caaggctccc ggatcctggc tcgtcacggt cccttcgttt
     1501 cgcgtcgaca tcgagcggga ggcggacctg atcgaggaaa tcgcccgctt tttcggatac
     1561 gaccgcatcc cctcggtcgt cccgccgctg gatgccttgg aacctattcc ctcgaaccgg
     1621 gaccggatcg accgcctctc ccgccggctc tttcattacg gattcgatga agtcgtcaac
     1681 gccagcttcg ccgacccgga gaaggaggct gttctgcaga cggaacggac gccggtgact
     1741 ctgcgcaatc cgttttccgt ccatgccgcc gctctccgga cgacgcttct cggcggcttg
     1801 ctgatcaatc tccggcacaa ccaaaaccac ggaaccgagg acgtccatat ttttgaaatc
     1861 ggcaggatct tccaccggat cgaggacgct ccgcctgccg aggagctttt cctcggcctc
     1921 ctgagcgccg ggccggtggg ccttccccac tggagccgtc cgccctcagc cgcgaatttc
     1981 ccccatctca agggcgccct ggaatccgcc ttggcggccc tccgctatga aacgcttgtc
     2041 tacgagccgg ctgaacaccc ggcgttcgcc gaggggtcgg ccctggccgt tctttacaag
     2101 ggggagaaga tcggcctttt agggcgtgtc cgcgagtcgg tctgcgaggc cgccgggacg
     2161 accggaccgg tcttcgccgc ggaaatcgac ttgggttgtc tcctggaaaa gaaacccgcg
     2221 gccttcgctt acgaaccgcc gcccaaagtt cccgccgttg tccgcgatct ttcgtttttc
     2281 gtctcccggg atatcttcta ccaggagatc aagtcggccg tggaacgggc cgcggtcccc
     2341 tacctggaat cgttcgacat catcgaccgg tacgccggcc cccatcatcc cgacggccgg
     2401 acatcgcttt cgatgcggtt tgtcttccgg aatccccaag ccacgctcct ggccgaggaa
     2461 gcggataaat ccgtccaaaa agtcttgaaa gtcttgaaat cggcgttcca gatcgagctt
     2521 cgaaaaggag acggcgcgtg accaatgaaa tcgaccgctt gaaaatcctc gaggataaaa
     2581 tcaccggggt gatcgatcat atccagcgct tgtcgacaga aaacgaaaaa ctcaagcagc
     2641 tggtcaagga tctcaaagcc gaaaaacggg attccgagga tcttgcccgg aaagtcgtca
     2701 aacttgacgg ggagctcaaa aaatacgaaa atgaaaggga agaaatgaag gggaagatcg
     2761 aagcgatcat ttcccagatc gacaagctgg gcatgtgatg agcgatatcg tcgagatcga
     2821 aatctacgga cgaacgtata aaatcagagt gaaaggtgag gaaaacgaag aatatatcag
     2881 ccgtctgacg tcctatgtgg accaaaagat gcaggaaatt gcggtgaagt cgcgatcgga
     2941 ggacccaacg aaaatcgccg tcctcgcggc tttgaatatc gccgatgact acttcgtcag
     3001 ccggaagcgg ttggaacaag tggacgaacg catcgaccgg atggaagccg aaatcgcgac
     3061 cgtggcgtcg aacctgttca aggatgatca tgcttaaccc gagcgaaaaa acgcccgccg
     3121 ccggacaccg gcggccttgg aacgtccttc ggaccggccc caccccgtaa gtcccccctg
     3181 ccggacggct cttcctcgct gcacttttgg gaaggaggag agaccgtgaa cattggaatt
     3241 ctttatatcg gcttggccgt cctgatcatc ctggccgcgg gtatcctttt cattcaggtc
     3301 aagcggacgt cgggggccaa gaagctgttc cgggccggcg aggaagcggc cgggctcgtc
     3361 ggacaagcca agagcgaggc ccagcgcctc gtctcccaag cccagaacca agcccgccaa
     3421 aagctggcct cggccgacgc cgagttcgag cggatgaccc aggacaagcg gcgccggctc
     3481 aacgaggtgg agaggcgcct cgtccacaag gaggagaatc tcgatcgcaa gatccagggg
     3541 ctcgaccgcc gggaccggga tatcgcgacc aaggagcgga gcctccttca gaaagagcag
     3601 gaaatcgaga atgaggcgaa gcggatctcc tcggtcatcc aggaccaact ggtcgagctc
     3661 gaacgcatct ccagcatgac ccaggaggaa gccaaggctc agatcatccg caaggtcgag
     3721 gacgaggccc gccttgaggc gatgcagacg ctcaagcggc tcgacgagga aacgcgggcc
     3781 aagagccgcc agctggcggc cgaggtgatc agcggagcga ttcaacgctc ggccgccgag
     3841 cacgtcgtcg agtcgacggt ctccgtcgtc gatcttccga gcgaggacat gaagggccgg
     3901 atcatcggcc gcgagggccg gaacatcagg gccctggaag tggccacggg cgtggacatc
     3961 atcgtcgatg acactccgga agcggtcatc ctttccagct tcgatccggt ccgccgggaa
     4021 gccgcccggc tggccatcca gcggctcgtc ctggacggcc gcatccatcc ggcccggatc
     4081 gaggaggtcg ttgaaaacgt caaagccgag ctcgaggaca agatccggca ggacggagaa
     4141 gcggcggcca tggaattgcg catccagaac ctccatcccg agctcatcaa gctgatgggg
     4201 aagctgaaat tccggacctc ctacggccag aacgtcctcc agcattccaa ggaagtcgcc
     4261 ttcctcagcg cagccatggc ccaagagctg ggcctgaaca cccaaacggc cctccgggcc
     4321 ggatttctcc acgacgtcgg caaggccatc gaccgcgagg tcgagggaac ccacaccgag
     4381 ctgagcgtcg agctctgccg gaagtacggc gagaatgaag cggtcgtcgc ggccatcgcc
     4441 tcccaccacc gggacgtgga tttcccctcc atcgaggcgg tcctggttca ggcggctgac
     4501 accctgtcgg cggcccggcc gggcgcccgc cgggagcttc tcgaaaatta catcaagagg
     4561 ctggaaaagc tcgaaaccct ggccaagggc ttcgagggcg tgtccaaggc gttcgccctt
     4621 caggccggac gcgagatccg gatcatggtc gacgccggca aggtcgccga cgagcgggcc
     4681 gggctgatcg ccaaggatat cgcggccaag atcaaaaacg agctggatta ccccggacag
     4741 atcaaggtga cggtcatccg ggagatgcgg gcggttgaat atgcccgata aatccggaaa
     4801 ggaaaagagc gggacggccg ttttgcggcc gtctttccgt gtcctgatca tcggggacgt
     4861 catcggccgg tcggggaggg aagccctgcg ccggcacctg ccggtgctcc gcgaccggca
     4921 tcgtccggcc ttcgtcgtgg ccaatggaga gaatgcggcc ggcggcgtcg gcttgaccga
     4981 ggagaccggc cgggagcttt tttccatggt cgacgtcctg acgagcggca accatatctg
     5041 ggacaagaaa gaaggaatcg cctacatgga gaaggaatcc cgggtcctcc ggccggcgaa
     5101 ttatcctccg cccgccccgg gccggggaag cgtggcggtc gccggccccg acgggttgaa
     5161 gctcggcgtc ctgaatctcc agggccgggt gttcatggag ccgatcgatg acccgttccg
     5221 ggcggccgac gcggcggttg aggagctccg gaaggagacg ccctgcctca tcgtcgattt
     5281 tcacgccgag gcgacgtcgg aaaaagtcgc cctgggctat tacttggacg gccgggtttc
     5341 ggccgtcgtc gggacgcata ctcatgttcc gacggccgac gggcgcctcc ttcccggcgg
     5401 gacggcctac gtcaccgatc tcggaatggc cggctcgcgg aattcggtca tcggaatccg
     5461 caaggagcag gccattttga agtttctgac gtcccgcccg attcgcttcg aaccggacaa
     5521 ggggggatcg tttttgtcgg gcgtcgtcgt cgaaatcgaa acggcgaccg gccgggccct
     5581 ctccatcgtc caagagatca tcgccgagga ttgaccgggg cccggaggcc aggccttgga
     5641 acgaattgaa tgaaccgggc cgtccgtata aaatagccgg ggagaagcga tgtccgggga
     5701 aaaagaaggc cgagggctcc cgcccgagga ggcgaagagc ggccgggatc tcgtccggga
     5761 gggcagcctc gttctccgcg accacgtcta ttcggtcacc gagatcaccc aggtcatcaa
     5821 ggccgctctc gaaaccgccg tcccccaggt ctgggtcgtc ggcgaaattt ccaactgccg
     5881 gcggcatccc tcgggtcata tttatttcac cctcaaggac gccggagcgg cgctttccgc
     5941 cgccgtttgg aaaagcaacg ccgcccgtct caagttcgag ttgcgggacg gccagcaggt
     6001 cgtctgccgc ggccggatcg acgtctaccc gccccggggg agctatcagc tcaacgtcga
     6061 ccaggtcgaa cccaagggca agggagccct ccagctcgcc ttcgagcagc tcaaggaaaa
     6121 gctccgggcg gaaggattgt tcgatcccgc ccgcaaaaaa aagatccctt tgctgccgaa
     6181 gacgatcggc gttgtgacct ccccgaccgg ggccgccctg atcgatatcc tccggacgat
     6241 cgaccggcgc ttcgccaagg tccggatcgt gatttatccg gcccgagtcc agggagcggg
     6301 ggcggcggcc gaaatcgtcg aggggctgga gttcctcgga ggccggccgg gcatcgacgt
     6361 gattatcgtc ggccggggcg gcggatcgat cgaggactta tgggccttta acgaggagcc
     6421 cgtcgcccgg gcgatcgccg cctctccggt cccggtcatc tcggccgtcg gccacgaggt
     6481 cgatttcacg atctccgatt tcgtcgccga catccgggcc tccaccccga cggccgccgc
     6541 cgaaatggtc gtggaaaaag aggagacgtt cgccgagcgg atcgacaatc tccggcgccg
     6601 ggcggggcag gccgcccggt tcggccttca gacgcgccgc ggcggcgtcg acgagctcgc
     6661 ccggcacggc gtcttcgagg gtttccagcg ccgcctctgg atgctggggc agcgcgtcga
     6721 tgagctggaa gggcgggcgt tcgatgtggt gaacgcggaa cgccggagaa tcgccgacgg
     6781 aaaagcgcgg gcggtcctgg ccgccgggcg catggccgcc gttctcggcc ggcggctcgg
     6841 ccaggcctcc ggccgggcgg ccctggtcga ggagaggatc ggcggcgcat ttcgggcgtt
     6901 attggcgaaa cggatgagcg aatgggagcg cctttcggct tcgctccagg ccctaagccc
     6961 gcttgatgtc ttgaagaaag gctatgccgt cgtttggaag gaaggaacgc cgcggattcc
     7021 ggtcacccgg gcggaaagcc tccggcccgg ggacgcagtc gcggtctcgt tccaccgggg
     7081 ggaattcatc gccgacgtgc ggacggtcga cccggtccgg ccggtcacgg tcccggacga
     7141 aagggagagg tcatcatgaa agaaaaggac aaaacgaaga agccggatct ggattttgag
     7201 aaggccttag ccgacttgga aggggtcgtg gaggagctgg aaaaaggcgg cctcagcctc
     7261 aacgagtcgc tcgcccggtt cgaacggggc gtcaggcttt cccggtttct ccgcaatgag
     7321 ctggacaaag ccgagaaaaa aatcgagatc ctcttgaagg acgaagccgg cgaggttcat
     7381 cccgagccct tcagcctgga ggaggccgaa agcggaaagc cgccggtggc caaaccggga
     7441 cccgcctcga ggaaaatccc tccgaaaacc gggacggaag acgacgattc cgagcttccg
     7501 ttctaaatcc cggggaaaag agggccgcgc tttgcctttc cgctctctcc ggagagggga
     7561 tcaggcctgt ttgccgatct cccggcggcg ggatttcatg ttataataac cttttatgag
     7621 aaggatgata tgcgccggat gagtggccga aaaacgggac ggaggcggat caagggctcc
     7681 gcttgacctc cccttcccgc gatgaaaaga atcctcgact cgatcgactc cccggccgac
     7741 ctgaagactc tgtccttggc cgatatgtcc cggctggccg tcgagatccg ggctgagatt
     7801 ctcgacgtcg tttcccgaaa cggcggccat ctgtcctcaa acttgggagt cgtcgagctc
     7861 acgttggccc tccaccgagt gttcgacgcc ccccgggatc tcatcgtctg ggacgtcggc
     7921 caccagacct acacccataa aatcatcacc gggcggcggg accggttcgg caccctccgc
     7981 cgcgaaggcg ggcttctcgg atttccctcg cgcgaggaaa gcccctatga tccctacaac
     8041 accgggcatg cctcgacggc cctctcggcc gccctggggc tggcggtcgc ccgggacatg
     8101 gccggagaga accgcctggt catcgccgtc gtcggggacg gctcgctcac cggcggggtg
     8161 tcctgggaag ccctcaacca gatcggcaac ctgcggatcc cgatgatcat catcctcaac
     8221 gacaatgaga tgtcgatctc tcagagcgtc ggagccatct ccaagtatct cggctatctg
     8281 gcctcgggcc agcattacct ccgggtcaag gacaaggcca aaaccctttt gaaatccgtt
     8341 ccgggagtgg gcggggcgat catccgggcc ggccgcgggc tcgaggaatt ggtcaaaaag
     8401 acgtttttcc cgggactcgt gttcgaagag ctcgggctgc ggtacgtcgg acccgtccag
     8461 ggccacagcc ttccctcgct tttggaggtg tttgaacggg ccaagaaaga agccgagggg
     8521 ccggtgctga ttcattgcgt gacccggaaa ggccgcggtt acactccggc tcaaaacagc
     8581 cccgagcatt tccacggggc tccgccgttc gacgtcgagt ccggcgagcc ggctttctcc
     8641 ggaacccatc cgtcctactc ggaggtcttc ggggaaaccc tgctccgaat cgccggcgag
     8701 gatgagcggg tcgtggccat caccgcggcc atgcgcgagg ggaccggctt gatggaattc
     8761 gccgcccggc gcccggccca gttttttgac gtgggcatcg ccgagcagca cgccgtcaat
     8821 ttcgccgccg gcctggcggc cggagggcgg cggccggtgt gcgccatcta ttcgacgttt
     8881 ctccagcggg cttacgacca gatttaccat gatgtctgtc tccagaagct ccccgtcgtt
     8941 ttcgtccttg accgggccgg cctcgttccc gacgacggcc cgacccacca gggcgtcaac
     9001 gacatcgctt atctgcggca catgccgaac atggttctcc tggccccccg ggacgaaaac
     9061 gagatgcggc acatgttgaa agccgcgttc ggctatgacc ggccggtggc cctccggttt
     9121 cccaagggcg aaatcacggg cgcggttccc gaaccgggct ggaaagacat ccctctcgga
     9181 aaggcggaga tactcaaaga cggggagaat ctcgtcctgg cctatggaag catggttctg
     9241 ccggccctgg aggcggccga acgggccgaa aaggaatcag ccgccgcggg agcagggctc
     9301 agtctctccg tcgccgacgc gagattttgc gcgcccttgg acgaagaatt gatcctccgg
     9361 ttcgcccgtt ccggccgggc gatcgcgacc gtcgaggagg gcgtactggc cggcggcttt
     9421 ggaagcgccg ttctggaatt gatcaaccgc cgcgggcttt acggcgtccc gatccggtgt
     9481 ttcggtcttc cggtcgagac gttccccgtg gggaagctcg gccagctccg ccgccggtac
     9541 gggcttgacg ccgacggctt ggcggccggc ttccggtctt ttttcagccg gctggcgaaa
     9601 acggaggacg gcggaccgac gtcctgaacg gcccggaggg gcgccctccc ctcgtgaaaa
     9661 tcatgaaaaa agcgcgcgcc gatgcgctcc tggtggaacg cggcttggcg gattccgtcg
     9721 ccaaggcccg ggccttaatc atggccggcc gggtcgcctc ggcgggccgc cagatcgaaa
     9781 aggccggcga gccgctggct ggcgacgtcc ccctctccct caaggccggc cggccgtatg
     9841 tcggccgcgg cggcttgaag ctggccgagg cgctggacgc cttccgtgtt tccccgagcg
     9901 ggcttgtctg cgccgacatc ggggtttcca ccggcggttt caccgactgt cttctccagc
     9961 gcggagcgct ccgggtttat gccgtggacg tgtcggtccg gcaaatcgac gatcgcctgc
    10021 gccgggaccg gcgcgtcgcc ttgatcgaga aaaacgcccg gctcctccaa ccgggggatt
    10081 tccccgagcc ggtcgggctg gttgtcatgg acgtctcctt catttcgatt ctcaagatcc
    10141 ttccggcctt ggccgccgtc ttgaggagcg gccgggagac ggccggaggg gaggagccga
    10201 ttcttctttc cttgatcaag ccccaattcg aggcggacag gagaagcgtc ggccgaggcg
    10261 gcgtcatccg gaatccggag atccatgacc gggtgctgcg gcgcgtggcc gcgggcgccc
    10321 ggccagcggg gttcttcctt tcgggactca tccgcttgaa cacccgcgga cagaaaggca
    10381 acgtggaatt tttcgggcgg ttcggcttga gcgccccggc ggtggaaccg ctcccgggcc
    10441 gcgtgttaga atggatttcc gcggccgtca aggaagcgga tcctgatccg ggaggaaatg
    10501 aaggatgaag accgttcggc gcgccggcat cgtcatcaag ccccacgctc cgggagtggc
    10561 cgaggtgatc gccggcctga ccgccgctct cgaggctcgg gggatcacgc ccgtgctgga
    10621 gcgggccgcg gccgaaaaag tcggccgagc cgacggtctg gcgcgcgaaa gcattccagc
    10681 cgcttgcgac ttggtcgtgg tcctgggagg ggacgggacg tttctcagcg tcgcccactc
    10741 cgccgcccgg gcggatgtcc cggtcatggg cgtcaacttg gggagcctcg gttttctgac
    10801 ggaaatcccc ctggcggaaa tgaccctcgc cctggatgcc gttctttcgg gacggacgga
    10861 catcatcagc ccgcggatga tgctctccac gacgttccgg gaaaaaacgg agctgaccct
    10921 caacgacatc gtcttcaata aggcggccaa ggcccggatg atccattacg tcctctcggt
    10981 caacggtttg gaaatcgccg ctctccgggc cgacggcctg gtggtggcca cgccgaccgg
    11041 cagcacggct tactcgcttt cggccggcgg gccgatcctc cagccgagcg tgccggcgat
    11101 cctcctgacg ccgatctgcc cccatacgct ttcgttccgg ccgacggtcg tttcctccga
    11161 ttccgtcatc cggatcgagg tccgcacgcc cggcgaggaa gtgtacctga ctctggacgg
    11221 ccagcggggc ggggagatgg acagcgaagg catcgtcgaa atccggaagg cagatcacgt
    11281 cctccggctg gtgacgtcgc cgcagcgaag atatttcggc cttcttcagg agaagctccg
    11341 gtggggctga aggccctcct tccggcgaaa accgggggat ccggcgcggc aacggatctt
    11401 gccggaagga gcgggcgatg atgaatcctc tcctccggag gaagccgagc gtcgttttgg
    11461 tctcggccga ggcttttccc ttcgccggca cccgggcttc cgcggccggg atgcaggctc
    11521 ttcccgaatc cctggcccgc ctgggattcg acgtctgcct ggtgactcca aagtaccgcc
    11581 ggccggcgat cgaagcgctt ccgctcacgc cggtcctccg gcgttttctc gttccggtcg
    11641 ggaaggacaa agtcccggct tcagccttcc gggccgaaac ggccatttcg cccttgccgc
    11701 ccgtggattt ttccgcggtg ccggaccccc cggcccgccg cttccgggcg tatttcatcg
    11761 acaacgtcaa gtatttcgac cgcgagcgga ttttcggctc ggagaaggcg ccgtacctcg
    11821 acaacgacga acggttcgcc ttcttctgcc gggccgtcgt cgaattcctc cgccgggccc
    11881 ggatccggat cgacatcctc cattgccatg actggccgtc cgccctgatt cccctctttc
    11941 tccggacggc ttatgatgga cagcgtccgt tccgcaagac cgcctccgtt
//
LOCUS       DAOOXH010000077        11424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_6007_length_11424_cov_8.462838, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000077 DAOOXH010000000
VERSION     DAOOXH010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 11424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11424
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6007_length_11424_cov_8.462838"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PKV64_08880"
     CDS             <1..518
                     /locus_tag="PKV64_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17229.1"
                     /translation="KYCDDCLSKELNITPRQQVNQICNKFAKAGKIMRNNSICDNCGK
                     QKLSNHIGVEFPFGQKAYVLKESIPLSKEIFGNLEEFDIEKIRTQVVHICHKIWIEKK
                     EDAAPHSISSVINILKNDHYLPDHQANMMLTLCSMRNECVYDDFKLGDREKAIAINAW
                     KIISDWWGNKK"
     gene            577..3249
                     /gene="mutS"
                     /locus_tag="PKV64_08885"
     CDS             577..3249
                     /gene="mutS"
                     /locus_tag="PKV64_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPN17230.1"
                     /translation="MDEPRLFADDPTTPMMEQYFRIKSAHPDALLFFRLGDFYEMFYE
                     DAAIGSRVLEIALTSRQKVPMCGVPYHAVAQYSAKLLRRGYKVAICEQVEDPRLAKGV
                     VKREVIKILTPGTAVEVDPEAAKENVFVAGLFVDPEGWGLALIDISSGEMGVLEGPSS
                     GTKRLVDELAKASPKEIVLPEGAEESIVPYLKNGDFKSIVKSPVEAWAFDFTQARHSL
                     LEHFKVSTLAGFGLEGKARAVSAAGGLLFYLKRVRKDSLALVHRLSFLPAGDSCVLDP
                     ATIRNLELVKNLRDQKVDNSLLDVIDFTVTPMGGRLLRNRLLGPLLDPEEINRRLDAV
                     GELLNDTIGRAEIRDDLKGILDLERLTGKIALSAAHPRDFVALKRSLLPLPGLKKKLA
                     SLSSAALAEIRESWDSAEDVAALLERAILDEPAFLLTEGGLFKDGYNADLDDLRKISR
                     SGKAFISTLEKRERDRTGIGSLKVRFNKNFGYFIEVTKANLGQVPPDYIRKQTLVGSE
                     RFLTPELKEYEEKVLHAEERIGELEFRLFGELRETVARETPRLLRIASAVARLDVSAG
                     LAELAARRDYVRPVVDGGDAVEIREGRHPVVESMNLEPFIPNDTHLDRADDQIHIITG
                     PNMGGKSTYLRQTALIVILAQMGSFVPAKSARIGVVDRIFTRIGAMDFLTVGQSTFMV
                     EMIETASILHNATPRSLILLDEIGRGTSTFDGLSLAWAVAEYLHDREEVRAKTLFATH
                     YHELTELALTHPRIKNAHVTVKEWKDEIVFLRKIAPGPSDQSYGIHVAKLAGIPAPVV
                     SRAREILFNLEKGELDEDGRPRLALSETVKSDPSQKMLFVDDRERAAFREIREALEDC
                     DPTRLTPLEALNLLDKMVEKVREASAPPPSIKND"
     gene            complement(3312..3671)
                     /locus_tag="PKV64_08890"
     CDS             complement(3312..3671)
                     /locus_tag="PKV64_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17231.1"
                     /translation="MEEMEISSWVTLDAVKKKAEIHEIVGNLQTGHSMSVFCQIEDDY
                     LELVYSDSAANTLRRFEDKDEFEIGLKQRKEEDGDAPYDDEKEFDEKSFDDDDVEDEE
                     DEEEYDEDNDEEEDEDT"
     gene            3885..5171
                     /locus_tag="PKV64_08895"
                     /pseudo
     CDS             3885..5171
                     /locus_tag="PKV64_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
     gene            complement(5363..7564)
                     /locus_tag="PKV64_08900"
     CDS             complement(5363..7564)
                     /locus_tag="PKV64_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HPN17232.1"
                     /translation="MRWEKLTVMSQEAIQAAQARADELGHAEVTPEHLLAVFLAQEEN
                     IVRAVLAKLGVSEARLQESLETVLSRRPKARGGEIRLSESLQRILGQAEKEADVLKDE
                     YVSTEHLFLAMVKDPRSEAGRLLAEAGITPETVLQALASVRGTQRITDPEPESKYQVL
                     AKYTRDFTEMARQGKLDPVIGREEEIRRVIQVLSRRTKNNPVLIGEAGVGKTAVVEGL
                     AQRIADGDVPASIKNKRLVALDVGALLAGTKFRGEFEDRLKAILKEIKESEGDIVLFI
                     DELHTIVGAGAAEGAMDASNLLKPALARGELHCVGATTINEYRKHIEKDAALERRFQP
                     VMVGEPTVEDTISILRGLKEKYEVHHGVQIKDAALVAAATLSNRYITTRFLPDKAIDL
                     MDEAASRIRMAIDSMPEEIDELERRIRQIEVERQALKKEDDPAGRDKLQRLGAELAGL
                     NERADVLKTQWKREKELIDEIKKLKAELDRLKNEEQAAERRGDLERVAEIRYGKKIEA
                     GKRLEKLAADLAAVQKNRKMLKEEVDEEDIAGVVSKWTGIPVSKMLESDLARLIKMED
                     ALRRRVIGQDEALRAVSDTIRRARAGIQDASRPLGSFIFLGPTGVGKTELARASSVLP
                     TPVGPRKMNEPSGREASWMPARARRMVSETARRASSCPMTRRRRASSILISRARSLSS
                     ILETGMPVHFETTPAMSSSSTSSFSIFRFFWTAARSAASFSSRFPASIFFP"
     gene            7754..8113
                     /locus_tag="PKV64_08905"
     CDS             7754..8113
                     /locus_tag="PKV64_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17233.1"
                     /translation="MEEMEISSWVTLDAVKKKAEIHEIVGNLQTGHSMSVFCQIEDDY
                     LELVYSDSAANTLRRFEDKDEFEIGLKQRKEEDGDAPYDDEKEFDEKSFDDDDVEDEE
                     DEEEYDEDNDEEEDEDT"
     gene            complement(8176..10848)
                     /gene="mutS"
                     /locus_tag="PKV64_08910"
     CDS             complement(8176..10848)
                     /gene="mutS"
                     /locus_tag="PKV64_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPN17234.1"
                     /translation="MDEPRLFADDPTTPMMEQYFRIKSAHPDALLFFRLGDFYEMFYE
                     DAAIGSRVLEIALTSRQKVPMCGVPYHAVAQYSAKLLRRGYKVAICEQVEDPRLAKGV
                     VKREVIKILTPGTAVEVDPEAAKENVFVAGLFVDPEGWGLALIDISSGEMGVLEGPSS
                     GTKRLVDELAKASPKEIVLPEGAEESIVPYLKNGDFKSIVKSPVEAWAFDFTQARHSL
                     LEHFKVSTLAGFGLEGKARAVSAAGGLLFYLKRVRKDSLALVHRLSFLPAGDSCVLDP
                     ATIRNLELVKNLRDQKVDNSLLDVIDFTVTPMGGRLLRNRLLGPLLDPEEINRRLDAV
                     GELLNDTIGRAEIRDDLKGILDLERLTGKIALSAAHPRDFVALKRSLLPLPGLKKKLA
                     SLSSAALAEIRESWDSAEDVAALLERAILDEPAFLLTEGGLFKDGYNADLDDLRKISR
                     SGKAFISTLEKRERDRTGIGSLKVRFNKNFGYFIEVTKANLGQVPPDYIRKQTLVGSE
                     RFLTPELKEYEEKVLHAEERIGELEFRLFGELRETVARETPRLLRIASAVARLDVSAG
                     LAELAARRDYVRPVVDGGDAVEIREGRHPVVESMNLEPFIPNDTHLDRADDQIHIITG
                     PNMGGKSTYLRQTALIVILAQMGSFVPAKSARIGVVDRIFTRIGAMDFLTVGQSTFMV
                     EMIETASILHNATPRSLILLDEIGRGTSTFDGLSLAWAVAEYLHDREEVRAKTLFATH
                     YHELTELALTHPRIKNAHVTVKEWKDEIVFLRKIAPGPSDQSYGIHVAKLAGIPAPVV
                     SRAREILFNLEKGELDEDGRPRLALSETVKSDPSQKMLFVDDRERAAFREIREALEDC
                     DPTRLTPLEALNLLDKMVEKVREASAPPPSIKND"
     gene            complement(10907..>11424)
                     /locus_tag="PKV64_08915"
     CDS             complement(10907..>11424)
                     /locus_tag="PKV64_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17235.1"
                     /translation="KYCDDCLSKELNITPRQQVNQICNKFAKAGKIMRNNSICDNCGK
                     QKLSNHIGVEFPFGQKAYVLKESIPLSKEIFGNLEEFDIEKIRTQVVHICHKIWIEKK
                     EDAAPHSISSVINILKNDHYLPDHQANMMLTLCSMRNECVYDDFKLGDREKAIAINAW
                     KIISDWWGNKK"
BASE COUNT         2226 a         3486 c         3486 g         2226 t
ORIGIN      
        1 ataaatattg cgacgattgt ttatctaaag agctaaacat tacccctcgc cagcaagtca
       61 atcaaatatg taataaattt gcaaaagctg ggaaaattat gagaaataac agcatttgcg
      121 ataattgcgg aaaacaaaaa ttatcaaatc acataggagt tgagttccca ttcgggcaaa
      181 aggcatatgt tcttaaggaa agtatccctt tatcaaaaga aatattcggc aatttagagg
      241 aattcgatat tgaaaagatc cgaactcaag ttgttcatat ctgccacaag atatggattg
      301 aaaaaaaaga agatgcggca ccgcatagta tttcttccgt gattaacatt ctaaaaaatg
      361 atcattacct accagatcat caagcgaata tgatgttgac tctttgcagt atgcgcaatg
      421 agtgcgtata tgatgatttt aaacttggcg atagagaaaa agcaattgct ataaatgctt
      481 ggaaaataat atcagattgg tggggaaata aaaaataatt ggatcatttt ctaatcccat
      541 tccaccccct tcccactatg ctataatccg ctcgaaatgg acgagccccg cttgttcgcc
      601 gacgacccga cgactcctat gatggagcag tatttccgga tcaagtcggc ccatccggac
      661 gctctcctgt tcttccgcct gggggatttc tacgagatgt tctacgagga cgcggccatc
      721 ggctcgcggg tcctcgaaat cgccctgacc tcccgccaga aagtcccgat gtgcggcgtg
      781 ccctaccacg ccgtcgccca gtactcggcc aaactcctcc gccgcggcta taaggtcgcg
      841 atctgcgagc aagtcgagga tccgcggttg gccaagggtg tcgtcaagcg cgaagtcata
      901 aagatcctga cgcccgggac cgcggtcgag gtcgacccgg aagcagccaa ggaaaacgtc
      961 ttcgtcgccg gtttgttcgt cgatccggaa ggatggggcc tggccctgat cgacatctcc
     1021 tcgggggaga tgggcgtcct ggaagggccg tcctccggga ccaaacgcct ggtcgacgag
     1081 ctggccaagg cttcgcccaa ggagatcgtc ctcccggaag gggccgagga atccatcgtc
     1141 ccgtatctta aaaacggcga cttcaagtcc atcgtcaaaa gccccgtcga ggcctgggcc
     1201 ttcgatttca cccaggcccg gcattccctt ctcgagcatt tcaaggtttc gaccctggcc
     1261 ggcttcggcc ttgaagggaa agcccgggct gtctcggccg ccggcgggct gctcttctac
     1321 ctgaaacggg ttcggaagga ctcgctggcc ctggtccacc gcctgtcctt ccttccggcc
     1381 ggcgattcct gcgtcctcga cccggccacg atccgaaacc tcgagctggt caaaaacctc
     1441 cgggaccaaa aggtcgacaa ctcgctcctg gacgtgatcg acttcaccgt gacgcccatg
     1501 ggcgggcgcc tcctccgaaa ccgcctgctc ggcccgctcc tcgaccccga ggagatcaac
     1561 cgacggctgg acgcggtcgg cgagctcctg aacgacacga tcggccgcgc ggagatccgc
     1621 gatgacctca agggtatcct ggacctggag cgtctgaccg gcaagatcgc cctttcggcc
     1681 gcccaccccc gcgactttgt cgccctcaag cgttcgctcc tgccgctccc gggtctgaag
     1741 aaaaagctcg cctcgctctc aagcgccgcc ctggccgaaa tccgcgagag ctgggacagc
     1801 gccgaggatg tcgccgccct cctcgagcgg gcgatcctgg acgagccggc ctttctcctg
     1861 actgagggcg gcctgtttaa agacggctac aacgcggatc ttgacgatct gcggaaaatc
     1921 agccgctccg gcaaggcgtt catttccacc cttgaaaagc gcgagcgcga ccgaaccggg
     1981 atcggctccc tcaaggtccg tttcaacaaa aacttcggct acttcatcga ggttaccaag
     2041 gccaacctgg gccaggttcc gcccgactac atccgaaagc agacgctcgt cggctcggag
     2101 cgcttcctga cgcccgagct caaggaatac gaggagaagg ttctccacgc cgaggaacgg
     2161 atcggcgagc tcgaattccg actgttcggc gagctgcggg agacggtggc ccgggagacg
     2221 ccgcggctcc tccggatcgc ctcggccgtg gcccggctcg acgtgtccgc cggcctggcc
     2281 gagctggccg cccgccggga ttatgtccgg ccggtggtcg acggcggcga cgcggtcgaa
     2341 atccgcgagg gccgccatcc cgtcgtcgaa tcgatgaatc tcgagccgtt catccccaac
     2401 gacacgcatc tcgaccgcgc cgacgaccag atccacatca tcaccggccc gaacatgggc
     2461 ggcaagtcga cctacctccg ccagacggcc ctgatcgtca tcctggccca gatgggctcg
     2521 ttcgtcccgg ccaagtcggc ccgaatcggc gtcgtcgacc ggatcttcac ccgcatcggg
     2581 gccatggatt tcctgaccgt cggccagtcc accttcatgg tcgagatgat cgaaaccgcc
     2641 tcgatcctcc acaacgccac tccccgctcg ctcatcctgc tggacgagat cgggcggggg
     2701 accagcacgt tcgacgggct gagcctggcc tgggcggtgg ccgaatatct ccacgaccgg
     2761 gaggaggtcc gggccaagac gctgttcgcg acccattacc acgagctgac cgagctcgcc
     2821 ctgacccatc cccggatcaa aaacgcccac gtcacggtca aggaatggaa ggacgagatc
     2881 gtcttcctcc ggaaaatcgc ccccggcccc tccgatcaaa gctacggcat ccacgtggcc
     2941 aagctggccg gcatcccggc gccggtggtt tcgcgcgccc gggagatcct cttcaacctg
     3001 gaaaagggag agctggacga ggacggccgg ccgcggctgg cgctgtccga gacggtcaag
     3061 tccgatccga gccagaagat gttgttcgtc gacgaccggg agcgggccgc tttccgggaa
     3121 atccgggagg cgctcgagga ctgcgatccg acgcggctca cgccgctgga ggccttgaat
     3181 ctattggaca agatggtcga gaaagtccgc gaggcgtcgg cgccgccgcc ctcgataaaa
     3241 aacgactaag cgccggacga aaacgtcttt ggggggatgg ggcgtaagaa aagggggagg
     3301 agtgcgagag gtcaggtgtc ctcgtcctct tcctcgtcgt tgtcctcgtc gtattcctct
     3361 tcgtcttcct cgtcctcgac gtcatcgtcg tcgaagcttt tttcgtcgaa ttccttttcg
     3421 tcgtcgtagg gggcgtcgcc gtcctcttcc ttccgctgct tgaggccgat ctcgaattcg
     3481 tccttgtcct caaaccggcg gagggtgttg gccgcggagt cgctgtagac cagctcgaga
     3541 taatcgtctt cgatctggca gaagacggac atggaatgac cggtttgcag gttcccgacg
     3601 atttcgtgga tttcggcctt ttttttgacc gcgtccaggg tcacccagct tgaaatttcc
     3661 atttcttcca tgatccgctc cgctcgactc cgttcgacaa atccaaaata gcaacttttt
     3721 tttgtttgtc aagaggattc cgggcgcgtt ccgacggccg aaaaaaagat gaaaaagggt
     3781 cttgacttga caaacaaaat tcatggactt atctaatgaa ttcaaagaac ggccgtgcgc
     3841 cgacgaagag gagtcgcccc atgagatggg aaaaattgac ggtcatgtcg caggaagcca
     3901 tccaggccgc ccaggcccga gccgacgagc tcggccacgc cgaggtgacg ccggagcacc
     3961 tgttggcggt tttcctggcg caggaggaaa acatcgttcg ggccgttctg gccaagctcg
     4021 gcgtatccga ggctcgcctc caggagagcc tggagacggt cctgagccgg aggccgaagg
     4081 cccgcggcgg ggaaatccgc ctctcggaga gcctgcagcg gattctcggc caggcggaaa
     4141 aggaagccga cgtccttaag gacgagtacg tctcgacgga gcatctcttc ctggcgatgg
     4201 tcaaggatcc gcggagcgag gccggccggc tgctggccga ggccgggatc actcccgaga
     4261 ccgtcctcca agctctggcc tcggtccggg ggacccagcg gatcaccgac cccgaaccgg
     4321 aaagcaagta ccaggtttta gccaagtaca cgcgcgattt caccgagatg gcccgccaag
     4381 gcaagctcga tccggtcatc ggccgggagg aggagatccg gcgggtcatc caggtccttt
     4441 cccggcggac caagaacaat cccgtcctca tcggcgaggc cggcgtcggg aagaccgcgg
     4501 tggtcgaggg actggcccag cggatcgccg atggggacgt gccggcctcg atcaagaaca
     4561 agcggctggt cgccctggac gtcggggcgc ttctagccgg gacgaagttc cggggcgagt
     4621 tcgaggaccg gttgaaggcc atcctcaagg aaatcaagga atcggaaggg gacatcgtcc
     4681 tgttcatcga cgagcttcac acgatcgtcg gggccggcgc ggccgagggg gcgatggacg
     4741 cctcgaacct cctcaagccg gccctggccc ggggcgagct tcattgcgtc ggggcgacga
     4801 ccatcaacga gtaccgcaag catatcgaaa aggacgccgc cctggagcgg cggttccagc
     4861 cggtgatggt cggggagccg acggtcgagg acacgatctc gatcctccgc ggccttaagg
     4921 aaaagtacga ggtccaccac ggcgtccaga tcaaggacgc ggctctggtc gccgcggcga
     4981 cgctctccaa ccggtacatc accacccggt ttctcccgga caaggccatc gacctgatgg
     5041 atgaggccgc ctcgcggatc cggatggcca tcgattcgat gcccgaggag atcgacgagc
     5101 tggagcggcg gatccggcag atcgaggtcg agcgccaagc cctgaaaaaa gaagacgacc
     5161 cggccggccg cgacaagctt caacggctcg gggccgagct ggccggcctc aacgagcggg
     5221 ccgatgtcct gaaaacccag tggaagaggg agaaggagct gatcgacgag atcaagaagc
     5281 tcaaggccga gctggaccgg ctgaaaaacg aggagcaggc ggccgagcga cggggcgacc
     5341 tggagcgcgt ggccgagatc cgctacggga aaaagatcga ggccgggaaa cggctcgaaa
     5401 agctggcggc cgacctggcc gcggtccaga aaaatcggaa gatgctgaag gaggaagtgg
     5461 acgaggagga catcgccggc gtcgtttcga agtggaccgg catccccgtt tccaagatgc
     5521 tggagagcga cctggcccgg ctgatcaaga tggaggacgc cctgcggcgc cgggtcatcg
     5581 ggcaggacga ggccctccgg gcggtttcgg acaccatccg ccgggcccgg gccggcatcc
     5641 aggacgcctc ccggccgctc ggctcgttca tcttcctggg gcccaccggc gtcggcaaga
     5701 cggagctggc ccgggccagc tccgtcttgc cgacgccggt gggccccagg aagatgaacg
     5761 agccgagcgg ccgggaggcg tcctggatgc cggcccgggc ccggcggatg gtgtccgaaa
     5821 ccgcccggag ggcctcgtcc tgcccgatga cccggcgccg cagggcgtcc tccatcttga
     5881 tcagccgggc caggtcgctc tccagcatct tggaaacggg gatgccggtc cacttcgaaa
     5941 cgacgccggc gatgtcctcc tcgtccactt cctccttcag catcttccga tttttctgga
     6001 ccgcggccag gtcggccgcc agcttttcga gccgtttccc ggcctcgatc tttttcccgt
     6061 agcggatctc ggccacgcgc tccaggtcgc cccgtcgctc ggccgcctgc tcctcgtttt
     6121 tcagccggtc cagctcggcc ttgagcttct tgatctcgtc gatcagctcc ttctccctct
     6181 tccactgggt tttcaggaca tcggcccgct cgttgaggcc ggccagctcg gccccgagcc
     6241 gttgaagctt gtcgcggccg gccgggtcgt cttctttttt cagggcttgg cgctcgacct
     6301 cgatctgccg gatccgccgc tccagctcgt cgatctcctc gggcatcgaa tcgatggcca
     6361 tccggatccg cgaggcggcc tcatccatca ggtcgatggc cttgtccggg agaaaccggg
     6421 tggtgatgta ccggttggag agcgtcgccg cggcgaccag agccgcgtcc ttgatctgga
     6481 cgccgtggtg gacctcgtac ttttccttaa ggccgcggag gatcgagatc gtgtcctcga
     6541 ccgtcggctc cccgaccatc accggctgga accgccgctc cagggcggcg tccttttcga
     6601 tatgcttgcg gtactcgttg atggtcgtcg ccccgacgca atgaagctcg ccccgggcca
     6661 gggccggctt gaggaggttc gaggcgtcca tcgccccctc ggccgcgccg gccccgacga
     6721 tcgtgtgaag ctcgtcgatg aacaggacga tgtccccttc cgattccttg atttccttga
     6781 ggatggcctt caaccggtcc tcgaactcgc cccggaactt cgtcccggct agaagcgccc
     6841 cgacgtccag ggcgaccagc cgcttgttct tgatcgaggc cggcacgtcc ccatcggcga
     6901 tccgctgggc cagtccctcg accaccgcgg tcttcccgac gccggcctcg ccgatgagga
     6961 cgggattgtt cttggtccgc cgggaaagga cctggatgac ccgccggatc tcctcctccc
     7021 ggccgatgac cggatcgagc ttgccttggc gggccatctc ggtgaaatcg cgcgtgtact
     7081 tggctaaaac ctggtacttg ctttccggtt cggggtcggt gatccgctgg gtcccccgga
     7141 ccgaggccag agcttggagg acggtctcgg gagtgatccc ggcctcggcc agcagccggc
     7201 cggcctcgct ccgcggatcc ttgaccatcg ccaggaagag atgctccgtc gagacgtact
     7261 cgtccttaag gacgtcggct tccttttccg cctggccgag aatccgctgc aggctctccg
     7321 agaggcggat ttccccgccg cgggccttcg gcctccggct caggaccgtc tccaggctct
     7381 cctggaggcg agcctcggat acgccgagct tggccagaac ggcccgaacg atgttttcct
     7441 cctgcgccag gaaaaccgcc aacaggtgct ccggcgtcac ctcggcgtgg ccgagctcgt
     7501 cggctcgggc ctgggcggcc tggatggctt cctgcgacat gaccgtcaat ttttcccatc
     7561 tcatggggcg actcctcttc gtcggcgcac ggccgttctt tgaattcatt agataagtcc
     7621 atgaattttg tttgtcaagt caagaccctt tttcatcttt ttttcggccg tcggaacgcg
     7681 cccggaatcc tcttgacaaa caaaaaaaag ttgctatttt ggatttgtcg aacggagtcg
     7741 agcggagcgg atcatggaag aaatggaaat ttcaagctgg gtgaccctgg acgcggtcaa
     7801 aaaaaaggcc gaaatccacg aaatcgtcgg gaacctgcaa accggtcatt ccatgtccgt
     7861 cttctgccag atcgaagacg attatctcga gctggtctac agcgactccg cggccaacac
     7921 cctccgccgg tttgaggaca aggacgaatt cgagatcggc ctcaagcagc ggaaggaaga
     7981 ggacggcgac gccccctacg acgacgaaaa ggaattcgac gaaaaaagct tcgacgacga
     8041 tgacgtcgag gacgaggaag acgaagagga atacgacgag gacaacgacg aggaagagga
     8101 cgaggacacc tgacctctcg cactcctccc ccttttctta cgccccatcc ccccaaagac
     8161 gttttcgtcc ggcgcttagt cgttttttat cgagggcggc ggcgccgacg cctcgcggac
     8221 tttctcgacc atcttgtcca atagattcaa ggcctccagc ggcgtgagcc gcgtcggatc
     8281 gcagtcctcg agcgcctccc ggatttcccg gaaagcggcc cgctcccggt cgtcgacgaa
     8341 caacatcttc tggctcggat cggacttgac cgtctcggac agcgccagcc gcggccggcc
     8401 gtcctcgtcc agctctccct tttccaggtt gaagaggatc tcccgggcgc gcgaaaccac
     8461 cggcgccggg atgccggcca gcttggccac gtggatgccg tagctttgat cggaggggcc
     8521 gggggcgatt ttccggagga agacgatctc gtccttccat tccttgaccg tgacgtgggc
     8581 gtttttgatc cggggatggg tcagggcgag ctcggtcagc tcgtggtaat gggtcgcgaa
     8641 cagcgtcttg gcccggacct cctcccggtc gtggagatat tcggccaccg cccaggccag
     8701 gctcagcccg tcgaacgtgc tggtcccccg cccgatctcg tccagcagga tgagcgagcg
     8761 gggagtggcg ttgtggagga tcgaggcggt ttcgatcatc tcgaccatga aggtggactg
     8821 gccgacggtc aggaaatcca tggccccgat gcgggtgaag atccggtcga cgacgccgat
     8881 tcgggccgac ttggccggga cgaacgagcc catctgggcc aggatgacga tcagggccgt
     8941 ctggcggagg taggtcgact tgccgcccat gttcgggccg gtgatgatgt ggatctggtc
     9001 gtcggcgcgg tcgagatgcg tgtcgttggg gatgaacggc tcgagattca tcgattcgac
     9061 gacgggatgg cggccctcgc ggatttcgac cgcgtcgccg ccgtcgacca ccggccggac
     9121 ataatcccgg cgggcggcca gctcggccag gccggcggac acgtcgagcc gggccacggc
     9181 cgaggcgatc cggaggagcc gcggcgtctc ccgggccacc gtctcccgca gctcgccgaa
     9241 cagtcggaat tcgagctcgc cgatccgttc ctcggcgtgg agaaccttct cctcgtattc
     9301 cttgagctcg ggcgtcagga agcgctccga gccgacgagc gtctgctttc ggatgtagtc
     9361 gggcggaacc tggcccaggt tggccttggt aacctcgatg aagtagccga agtttttgtt
     9421 gaaacggacc ttgagggagc cgatcccggt tcggtcgcgc tcgcgctttt caagggtgga
     9481 aatgaacgcc ttgccggagc ggctgatttt ccgcagatcg tcaagatccg cgttgtagcc
     9541 gtctttaaac aggccgccct cagtcaggag aaaggccggc tcgtccagga tcgcccgctc
     9601 gaggagggcg gcgacatcct cggcgctgtc ccagctctcg cggatttcgg ccagggcggc
     9661 gcttgagagc gaggcgagct ttttcttcag acccgggagc ggcaggagcg aacgcttgag
     9721 ggcgacaaag tcgcgggggt gggcggccga aagggcgatc ttgccggtca gacgctccag
     9781 gtccaggata cccttgaggt catcgcggat ctccgcgcgg ccgatcgtgt cgttcaggag
     9841 ctcgccgacc gcgtccagcc gtcggttgat ctcctcgggg tcgaggagcg ggccgagcag
     9901 gcggtttcgg aggaggcgcc cgcccatggg cgtcacggtg aagtcgatca cgtccaggag
     9961 cgagttgtcg accttttggt cccggaggtt tttgaccagc tcgaggtttc ggatcgtggc
    10021 cgggtcgagg acgcaggaat cgccggccgg aaggaaggac aggcggtgga ccagggccag
    10081 cgagtccttc cgaacccgtt tcaggtagaa gagcagcccg ccggcggccg agacagcccg
    10141 ggctttccct tcaaggccga agccggccag ggtcgaaacc ttgaaatgct cgagaaggga
    10201 atgccgggcc tgggtgaaat cgaaggccca ggcctcgacg gggcttttga cgatggactt
    10261 gaagtcgccg tttttaagat acgggacgat ggattcctcg gccccttccg ggaggacgat
    10321 ctccttgggc gaagccttgg ccagctcgtc gaccaggcgt ttggtcccgg aggacggccc
    10381 ttccaggacg cccatctccc ccgaggagat gtcgatcagg gccaggcccc atccttccgg
    10441 atcgacgaac aaaccggcga cgaagacgtt ttccttggct gcttccgggt cgacctcgac
    10501 cgcggtcccg ggcgtcagga tctttatgac ttcgcgcttg acgacaccct tggccaaccg
    10561 cggatcctcg acttgctcgc agatcgcgac cttatagccg cggcggagga gtttggccga
    10621 gtactgggcg acggcgtggt agggcacgcc gcacatcggg actttctggc gggaggtcag
    10681 ggcgatttcg aggacccgcg agccgatggc cgcgtcctcg tagaacatct cgtagaaatc
    10741 ccccaggcgg aagaacagga gagcgtccgg atgggccgac ttgatccgga aatactgctc
    10801 catcatagga gtcgtcgggt cgtcggcgaa caagcggggc tcgtccattt cgagcggatt
    10861 atagcatagt gggaaggggg tggaatggga ttagaaaatg atccaattat tttttatttc
    10921 cccaccaatc tgatattatt ttccaagcat ttatagcaat tgctttttct ctatcgccaa
    10981 gtttaaaatc atcatatacg cactcattgc gcatactgca aagagtcaac atcatattcg
    11041 cttgatgatc tggtaggtaa tgatcatttt ttagaatgtt aatcacggaa gaaatactat
    11101 gcggtgccgc atcttctttt ttttcaatcc atatcttgtg gcagatatga acaacttgag
    11161 ttcggatctt ttcaatatcg aattcctcta aattgccgaa tatttctttt gataaaggga
    11221 tactttcctt aagaacatat gccttttgcc cgaatgggaa ctcaactcct atgtgatttg
    11281 ataatttttg ttttccgcaa ttatcgcaaa tgctgttatt tctcataatt ttcccagctt
    11341 ttgcaaattt attacatatt tgattgactt gctggcgagg ggtaatgttt agctctttag
    11401 ataaacaatc gtcgcaatat ttat
//
LOCUS       DAOOXH010000078        11423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_6008_length_11423_cov_7.685697, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000078 DAOOXH010000000
VERSION     DAOOXH010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 11423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11423
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6008_length_11423_cov_7.685697"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PKV64_08920"
     CDS             complement(<1..710)
                     /locus_tag="PKV64_08920"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HPN17236.1"
                     /translation="MNLSIPSLVRIALAAAVLAAGPAAAAAVQDAGPKPLPVCYEELT
                     SPDFVRAVEASRSTCVVPVGILEKHGPHLPLGTDLYECRETSLRAAAKEYAIVFPPYY
                     FGQIFEARHQPGTIAYSSHLILDLLQETCEELARNGLKKIILVNGHGGSEQLMQYFCQ
                     IQLARKKDYVVYLFQPEPDAAQQARIDQMRRTTLDGHAGEMEPSQMLAIRPDLVRLDQ
                     AGTQSGENQDRLAGLKHA"
     gene            1171..2078
                     /locus_tag="PKV64_08925"
                     /pseudo
     CDS             1171..2078
                     /locus_tag="PKV64_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
     gene            2358..2765
                     /locus_tag="PKV64_08930"
     CDS             2358..2765
                     /locus_tag="PKV64_08930"
                     /inference="COORDINATES: protein motif:HMM:NF023380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HPN17237.1"
                     /translation="MRVRGKAGAVEGLEFTERIGPVDDYARTDRPLPAPRVEASLDPA
                     LYDRLTGVYELSPDFTITITKEDGRLMGMATGQPKVELYPESETDFFLKVADARIVFT
                     LDASGQAVGLTLYQNGLALPAKKIKSSFETPFS"
     gene            complement(3087..4661)
                     /locus_tag="PKV64_08935"
     CDS             complement(3087..4661)
                     /locus_tag="PKV64_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPN17238.1"
                     /translation="MSNDPPSGPAPAPPDDGNISRACPEDRPNILPGLLEKARAAQRD
                     WAALSFRARAGHVLAVRDAIVAEADRIAEIISRDNGKTRIDALSAEVLPAAMSATYYA
                     KKAAGFLKPKKIRAGNILFLNKKSRLEHVPYGVIGIISPWNYPFAIPFHEIAMAFMAG
                     NAVLLKAASRTPEVARLIRDIIGAGRFPDGLFHIVNMAGPVAGQAFIDAGIDKLFFTG
                     SVAVGKKLMAAAAPRLLPLSLELGGNDPMIVCRDANLRRAAAGAAWAGYSNAGQSCGG
                     VERIYVEKDVYEEFVAILGGITESLTYGIDAGGGVDVGAVATKKQLAAIRAHLEDALG
                     KGARKTYFSKRLKGSADGLFFPPMVLENVDESMITMRDETFGPIVAVARVESVEEAIE
                     KANRSNLGLTASVWTRDRNKGRAIASRLEAGTVTINDHLMSHGLAETPWGGFKESGLG
                     RTHGALGLEEMTRTRVVVDDALPFVQKNMWWYPHDRSVYAGLRGGLDFLYRKTISLRM
                     KGLAKLLRAFVRTFQK"
     gene            complement(4702..6831)
                     /locus_tag="PKV64_08940"
     CDS             complement(4702..6831)
                     /locus_tag="PKV64_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPN17239.1"
                     /translation="MKSTRFPSRRTAAGIILAAFLAAGFLQAQEIPRPPAAEKIRKEL
                     AIHGQTRVDEYYWLNERANPKVVEYLKAENAYAEAVLKPTESLQEKLVAEMNGRIQPT
                     DMSVPYFENGYWYYTRFEAGRDYPLLCRKAKTLQGREEILLDGNKMAAGTSYFQIGGT
                     AVSPDNKILAYGVDTLSRRQYKLRFKNLKTGKTYPEEIPMTAGYAVWADDNKTVFYPV
                     IDDSLRSYKIFRHAVGAPASADVEIYHEEDPTFALGLDKSRSKKYIFLVASSTLTTEY
                     RFLEAARPLDGFAVFQPRTRGLEYNVDHLGGVFTILTNDQARNFRLMEADPGRTARGQ
                     WREVIPHRDDVYLTGFHLFDDFLVLREQKGGLSHLRVIPKGEKEGYDIPFPDKAYMVF
                     AAPTPEPGTALLRYHYNSLTTPPAVIEFNMKTRESSILKSQDVPGFNPDDYATERLSA
                     PAYDGTLVPISVVYRKGARDKGPAPLLLYGYGSYGYSTDPGFNAPRISLLDRGFLVAL
                     AHIRGGSEMGRSWYEDGKLLKKMNTFTDFISCGEFLVRHGYTAPDRLFAQGGSAGGLL
                     MGAVVNLRPDLFKGILAGVPFVDVVTTMLDDSIPLTTSEYDEWGNPADPVFYEYMMTY
                     SPYDNVRPKAYPAMLVTTGLHDSQVQYWEPAKWVARLREIKTDDHILLLKTNLDAGHG
                     GASGRFRSLREKAVQYAFILDLAGIKE"
     gene            complement(6855..8033)
                     /locus_tag="PKV64_08945"
     CDS             complement(6855..8033)
                     /locus_tag="PKV64_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17240.1"
                     /translation="MRRPFVGAILGMIVFLAGPGWGAAQAVKIRIIVDEGHVRLLPSP
                     QSSLLKTVPKGAVFPADDIENDWYRVFLPGGRGEYDLIGFIHRDEAEKVETASLRPEE
                     GPAVPEPSRRPLSLKISGGTARCRTGFPNTVREYLGAYFRAEAAAAKNLVVLQGDIPP
                     VHNGYNAAAELLVEFGGLGLGLGAEYVRVESTGSMNFATKHWTPDLYSFSMTDRLTVV
                     PVRANVYADVLRSRLGRILVYGGAGLYFGHFSDVWSGVNEKGETLYFSQRADASALGF
                     QAGLEIEIRLSERLALVAEAAGRRAVIDGFTGTAEGTPGWFHPKSGISEEPRSGILYS
                     YELQMTTDGPWIPELLIYEALPTAKSLGLSSRQRLRGAAEARLDFTGYSARLGLRFKF
                     "
     gene            8215..>11423
                     /locus_tag="PKV64_08950"
     CDS             8215..>11423
                     /locus_tag="PKV64_08950"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HPN17241.1"
                     /translation="MKPSLKKAVPAVLLLSFLSTVPGSAVRLKPGQDKPKVSTADLWK
                     LVEAADRDGLPRTAIEHLKEIVRLSLEEKREGEALRALTRRLVLESVVEGNKPGARVA
                     RIREEIDQAPEGMKTMLRTILAQWYRHYFSRNRWRFAQRSATAGGIDEKDFTTWDLRR
                     IVREIDALYQDILKDEASLKRTPVSVYRDFLEPGDPPESLRPTMFDFVAFEALGFYVS
                     GERAAAAPEDAFVLEADSPVLGPADEFILYRPETTDEDSPALRALRLYQSLLEFHRGD
                     RDRDVFLDADLHRLEAMRNLAAGESASARYLERLKEIAEKNPASAFSSLADYLRAREL
                     KNQGDLVQAYALADRGRKAHPASLGGRNCDALISDIASRDFDVRNERVVSPGTSSVLD
                     VRYRNLTALHFRAVRDDFEARLAAENAGDLFWPGDEPVRELLEREPAAAWSADLPATA
                     DFQARAERVAAPALPPGFYRLLVSADPAFSRGKNKIQAGSFWVSEWGIVTAGAGRALE
                     GFVVRGVTGDPVSGASVSLYDWDYGTDRLVKRGEGRTDDAGAFALAAPESYSNRLLVA
                     RGEKGIEVVETQVLTGSGRPASYERERTVFFTDRSLYRPGQTIHFKGLCLKADENRAD
                     YRILPRRNVRVVLRDANAQEASVLTLTTNDFGSFSGAFTAPADRLTGAMTISTENPRG
                     SCPIRVEEYKRPKFQVKIDVPDREFRLNDEISVPAEAMSYAGAPVDGALVKYRVIREV
                     RYPVWWWWTTPARGESREIAHGTSRTDAEGKFMVVFKAVPDPKSSPSSQPVFTFTVTA
                     DVTDGAGETRSAAGTIRAGYTALEAGLAGSDWQEKGEPVVLTATAATLNGKPAAAEGV
                     VEVYALRGPGRPVPRDLFAESGDRGRVRPSGSAASGDSETMDWRRWPEGRLAGRKEFA
                     VSAVEGSPCRLSWDLPPGAYRAILKTADRYGTAVESRFAFLVLDPAADRFAVPVPFHA
                     AARSGSVEPDGTFELFWGTGYERGPVLIEVHRDGRRLQRYWTRGTATQAVVRVPVTEA
                     FRGGFVVSAAIVKDNRLYREDRRVSVPWTNKRLDLRWKT"
BASE COUNT         1927 a         3623 c         3786 g         2087 t
ORIGIN      
        1 taagcgtgtt tcaggccggc cagccggtcc tggttctctc cgctttgcgt tccggcctgg
       61 tcgaggcgga cgaggtcggg acggatggcc agcatctggg agggttccat ctcgccggca
      121 tggccgtcga gcgttgtccg gcgcatttgg tcgatccggg cctgctgggc ggcgtccggc
      181 tcgggttgga acaggtagac gacataatcc tttttccggg cgagctgaat ctggcagaag
      241 tactgcatca actgctcgct gccgccgtgg ccgttgacca ggatgatctt cttgaggccg
      301 ttgcgggcca gttcctcgca cgtttcctgg agcaggtcga ggatcagatg ggagctgtag
      361 gcgatcgtcc cgggctggtg gcgggcttcg aaaatctgcc cgaaataata aggcggaaag
      421 acgattgcgt attccttggc ggccgcccga agcgacgtct cccggcactc atagagatcg
      481 gtgccgaggg gaaggtgggg gccgtgcttt tccaggatgc ccacgggaac gacgcaggtc
      541 gaacgggacg cctcgaccgc ccggacgaag tccggagagg tcagctcctc ataacagaca
      601 ggcaggggct tcgggccggc atcctgaacg gcggccgcgg cggccggacc ggcggcgaga
      661 acggccgcgg cgagggcgat ccggacgagg gatggaatcg agagattcat gcgaacctcc
      721 tttcggcgtt cgatgatttc tccttttata tatcccccgt ccgggcaagt caacgccgca
      781 caacggacgg tcagggcgac gggaactctt ccgcctcggt cgttcctcgc tcgctcgacg
      841 ggccggatcc cgtccctcaa aacgaaggca tggtcgttcg tttaagggcg atgtcgtcgc
      901 cgccggccgg tccggcaccc gtttcccggc tttgccgccc ggcgatcttc cggcggccgc
      961 ggaaacgcga atcccgagtc ttgtgtctcg ctcgttttgc ctctacaatc ggacgcaagg
     1021 agcccgatta tgaagaacat ccggatctgc ctcgccgcgg cggttttggc cgcgttcgca
     1081 gcgcttttcc tctccccgtc ggccgttttc gcccgaaatc cggccgtgac gcgggccgcg
     1141 tccgcggccg atgacgcctt ggccggccga atggacgcct ttctctcctc gatctacaag
     1201 cccggcgagc ccggggcggc cgtcctggtc aaaaaaaacg gccggattct cctgcggaaa
     1261 ggctacggcc tggccgacat ggagctcggc gtccccattg ctcccgacat ggtcttccgc
     1321 ctcggatcga tcaccaagca gttcacggcc gttggaatcc tcaagctggc cgagcagggc
     1381 aagctgtccc tcgacgacga tgtcaccgcg tttctccccg gctatccgac gggcggaaag
     1441 gccgtcacct caagcgtctg ctgacccaca cctcgggcct cgtcagctat accgatctgc
     1501 cggaatggct gccgctccgg cggaaggaca tgtcgctcga ggagcttgtg gatctgttca
     1561 aggacaagcc ggccgagttc agtccgggcg agcgctggca atactgcaac tccggatacg
     1621 ttctcctcgg ggcggtcatc gagaaggtct cggggatgac ctacgcggag ttcgtccgga
     1681 aggaaatctt cgagcccctg gagatgaggc attccgatta cgacagcccg acccggatca
     1741 tcccccgccg gatcggggga taccagaagg gaaacgaggg atacgaaaac gcgccctacc
     1801 tcagcccgac ccagccgtat gccgccgggg cgcttctctc ctcggtcgac gacatgaccg
     1861 tctggatcga gtcgcttctc gccggccgcc tgatcaagag agaaacgctg gagaaggctt
     1921 tcactcccgg cattctcaat aacggccgga cgaccgggta cggctacggc tggttcatct
     1981 cgacgtatca cggccgccgg atcatcgagc acggcggggg aatcaacggc ttcatcacgg
     2041 ccatgttgtt cgcccccgag gacggcgttt cggtcatcct tttaaccaac gcgatcggcg
     2101 acgggcggga tccggaagcc gacgccttcc gcctggcggc tctcgccttg ggagatcctt
     2161 acgtcgatcc cgcgccggtc gttttggagg aaaaagacct cgagccgctc gtcggcgtct
     2221 atgtcaacga aaggggcgtt gaccgtttca tcacccggga gggaggccgc ctcttcgccc
     2281 agcgggccgg cggagccaaa tacgagctgt tccccctctc ggcggacgag tatttcttca
     2341 aggattcgtt gagccggatg cgggtccggg ggaaagccgg agcggtcgag ggcttggagt
     2401 tcaccgagcg gatcgggccg gtcgacgatt atgcgcggac ggaccggccg cttcccgccc
     2461 cccgggtgga ggcctctctc gatccggccc tctacgaccg gctgaccggc gtctatgagc
     2521 tcagcccgga tttcacgatc acgatcacca aggaagacgg ccggttgatg gggatggcga
     2581 ccggccagcc caaggtcgag ctttatcccg agtcggaaac cgatttcttt ctcaaggtcg
     2641 ccgacgcccg gatcgtcttc accctggatg cctcggggca ggccgtcggc ttgacgctct
     2701 atcaaaacgg cctggccctc cccgcgaaaa aaatcaagtc gtccttcgag acgccgttct
     2761 cctgaaccgc gggggatgtc caggcggccc gggcgctctt cgccgcccgg tcgtggatcg
     2821 caggccgggg cccctgttcg gccggcgggc cggggcgttc atcccgggcg ttgggatcga
     2881 ggccgctttc attctcctct cggcccggaa agcggatatc gtcttcggcc cggtcttcgt
     2941 tccccggccg tcgcggccgc ggctcgaaag agacgcccgc cgccgggaat cgggccgcgg
     3001 gctttctttc ggttctttac gggatgggag gccgcgcggc gcggccgaag ccgggatacg
     3061 aggtccgcgg ggagcggact cccacgttat ttttgaaagg tccggacgaa agcacgcagc
     3121 agcttggcca gccctttcat cctcagggaa atcgtcttcc ggtaaaggaa atcgagccct
     3181 ccccggaggc cggcgtaaac cgaccggtcg tgagggtacc accacatgtt cttctgaaca
     3241 aacggcaggg cgtcgtcgac gacgacccgc gtccgggtca tttcctccag gccgagggcg
     3301 ccgtgcgtcc ggccgaggcc ggattccttg aatccgcccc acggcgtttc ggccaggccg
     3361 tggctcatca ggtgatcgtt gatggtcacc gtgcccgctt ccagccggga agcgatcgcc
     3421 cgccccttgt tccggtcgcg cgtccagacc gaggcggtca agccgaggtt cgagcggttg
     3481 gccttctcga tcgcttcctc gacgctctcc acccgggcga cggccacgat cgggccgaac
     3541 gtttcgtccc gcatcgtgat catcgattcg tcgacgtttt ccaggaccat cggggggaag
     3601 aacaacccgt ccgcgcttcc cttcaggcgc ttggagaaat acgttttccg ggctcccttg
     3661 cccagggcgt cctccagatg ggcccggatg gccgcgagct gctttttcgt ggccaccgcc
     3721 ccgacgtcga cgccgccgcc cgcgtcgatc ccgtacgtga gggattcggt gatcccgccc
     3781 aggatcgcga cgaactcctc atagacgtcc ttttccacgt aaatgcgctc gaccccgccg
     3841 caggattggc cggcgttgga gtatcccgcc caggccgccc cggccgccgc ccggcggagg
     3901 ttggcgtccc ggcagacgat catcgggtcg ttgcccccga gctcgagcga cagcggaagc
     3961 aggcgcggcg ccgccgcggc catcagcttc ttgccgacgg cgacggaccc ggtgaaaaac
     4021 agcttgtcga tcccggcgtc gatgaacgcc tgcccggcga cgggcccggc catgttcacg
     4081 atatgaaaaa gcccgtcggg aaaccggccg gcgccgatga tatcccggat gagccgggcg
     4141 acttccgggg tgcggctggc ggctttcaac aggacggcgt ttccggccat gaaggccatg
     4201 gcgatctcat ggaaggggat ggcgaacgga taattccagg ggctgatgat cccgatgacc
     4261 ccgtaaggga catgctcgag acgggatttc ttgttgagga agaggatatt ccccgcccgg
     4321 atctttttcg gcttcaggaa tccggccgct tttttggcgt aataggtcgc ggacatggcc
     4381 gccggaagga cctcggcgct caaggcgtcg atccgggttt ttccgttgtc ccgggagatg
     4441 atctcggcga tgcggtcggc ctcggcgacg atcgcgtccc ggacggccag aacgtggccg
     4501 gcccgcgccc ggaagctcag ggcggcccag tctcgctggg ccgcccgcgc tttctccaaa
     4561 agacccggga ggatgttcgg ccggtcctcc ggacaagctc gggaaatatt tccgtcgtcc
     4621 ggaggggcgg gcgccggccc ggacggagga tcgttgctca ccgctgcccc tttattctca
     4681 gatgccgccg tccgattctt tttattcctt gatgccggcc agatcgagga tgaaggcgta
     4741 ctggacggcc ttctcccgca ggcttcggaa ccggcccgag gcgccgccgt ggccggcgtc
     4801 gagattggtc ttgaggagga ggatatggtc gtcggtcttg atttcccgga gccgggcgac
     4861 ccacttggcc ggctcccaat actggacctg cgaatcgtgg agcccggttg tgaccagcat
     4921 cgccgggtag gccttgggcc ggacgttgtc gtacggggaa tacgtcatca tgtactcgta
     4981 gaaaaccgga tcggcgggat tgccccattc gtcgtattcg gaggtcgtca aggggatgga
     5041 gtcgtcgagc atcgtcgtca ccacgtcgac gaagggaacg ccggccagga tgcctttaaa
     5101 gagatcgggc cggagattga cgaccgcgcc catgagcagc cccccggcgc tcccgccctg
     5161 ggcgaagaga cgatccgggg cggtataacc gtgacggacg agaaactcgc cgcaggagat
     5221 gaagtcggtg aaggtattca tcttcttgag gagctttccg tcctcgtacc aggatcggcc
     5281 catctcggac ccgccccgga tatgggccag ggcgacgagg aagccccggt cgaggagcga
     5341 gatccgcggc gcgttgaaac ccgggtcggt cgaatagccg taggacccgt agccgtagag
     5401 aagcaaggga gccgggccct tgtcccgggc gcctttccgg taaacgaccg agatcggcac
     5461 cagcgtcccg tcgtaggccg gggccgaaag gcgctccgtc gcgtagtcgt cgggattgaa
     5521 accggggacg tcctggctct tgaggatgga ggattcccgg gtcttcatgt tgaattcgat
     5581 aacggccggc ggcgtggtca aggagttgta atgataccgg agaagcgcgg tccccggctc
     5641 gggcgtcgga gcggcgaaga ccatgtaagc cttgtccgga aagggaatgt catagccttc
     5701 tttttccccc ttcgggatca cccggagatg ggacagcccg cctttctgct cgcgcaggac
     5761 caggaaatcg tcgaacaaat gaaatccggt gagatagacg tcgtcccggt gggggatgac
     5821 ctcgcgccat tggccgcggg ccgtccggcc ggggtcggct tccatcaggc ggaaattgcg
     5881 ggcttggtcg ttggtcaaaa tggtgaaaac gccgccgagg tggtcgacgt tgtattccag
     5941 gccgcgggtt ctcggctgga agacggcgaa gccgtcgagg ggccgggccg cctcgagaaa
     6001 tcggtattcg gtggtcagcg tgctggaggc gacgaggaag atgtattttt tcgagcggct
     6061 cttgtcgagc ccgagcgcga acgtcggatc ttcctcgtgg tagatctcga cgtcggccga
     6121 ggccggcgcg ccgacggcat gccggaagat tttataagac cggagggaat cgtcgatgac
     6181 cgggtagaaa acggttttat tgtcgtcggc ccagacggcg tatccggcgg tcatcgggat
     6241 ttcctcggga taggttttcc cggtcttgag gttcttgaag cggagcttgt attgacgccg
     6301 ggagagcgtg tccacgccgt acgccaggat cttgttgtcg gggctgacgg ccgtcccgcc
     6361 gatctggaaa tagctcgttc cggcggccat tttattgccg tccaggagga tctcctcccg
     6421 gccctgaagc gtcttggcct tgcggcagag aagcgggtaa tcccggcccg cctcgaaacg
     6481 ggtgtagtac cagtagccgt tttcaaaata cgggacggac atgtccgtcg gctggatccg
     6541 cccgttcatc tcggcgacca gcttctcctg gagagactcg gtcggcttca ggaccgcctc
     6601 ggcgtaggcg ttctcggcct tgaggtactc cacgaccttg ggattggccc tctcgttcag
     6661 ccagtagtac tcgtccaccc gggtctggcc gtggatcgcg agctccttgc ggattttctc
     6721 cgcggccggc ggacgcggga tctcctgggc ttgaagaaat cccgcggcca ggaacgccgc
     6781 caggatgatt ccggcggccg tccgccgcga gggaaaacgc gtcgatttca tgagtctctc
     6841 ctttgagatg aaaatcaaaa cttgaaccgg agccccagcc gggctgaata tccggtgaag
     6901 tcaagccggg cctcggccgc gccgcggagg cgctgccggg agctcaaccc caggcttttg
     6961 gccgtcggca aagcctcgta gatcaacagc tcgggaatcc acgggccgtc ggtcgtcatc
     7021 tgcagctcgt aggaatagag gatcccgctt cggggctctt ctgagattcc ggacttggga
     7081 tggaaccagc ccggcgtccc ctcggccgtg cccgtgaagc cgtcgatgac ggcccggcgg
     7141 ccggccgcct cggcgaccag ggcgaggcgt tccgacaggc ggatttcgat ttccaggccg
     7201 gcctgaaagc cgagggccga ggcgtcggcc ctctggctga aataaagggt ttcccctttc
     7261 tcgttgactc cgctccagac gtcggagaag tggccgaaat acagcccggc cccgccgtag
     7321 acgagaatcc ggccgagccg ggacctgagg acatcggcat aaacattggc ccggacgggg
     7381 acgaccgtca ggcggtcggt catggaaaac gaataaagat ccggcgtcca atgctttgtc
     7441 gcgaaattca tcgacccggt gctttccacc cggacgtatt ccgctcccaa gccgaggccg
     7501 aggccgccga attcgacgag gagctcggcg gccgcgttgt aaccgttgtg aacgggcggg
     7561 atgtccccct ggagaacgac caggttcttg gccgcggccg cctcggcccg gaaataagcg
     7621 ccgaggtatt cccggaccgt gttcgggaat cccgtccggc agcgggccgt cccgccggag
     7681 atcttgaggc tgaggggacg ccgggacggc tccggaacgg cgggcccttc ctcgggtcga
     7741 agggacgccg tttcgacctt ctcggcctcg tcccgatgga tgaagccgat caggtcgtat
     7801 tcgccccgcc cgcccggaag aaagacgcgg taccaatcgt tctcgatgtc gtcggccggg
     7861 aaaacggctc ccttcggcac ggttttcaag agcgagcttt gcgggctggg aagaagccgg
     7921 acatggccct cgtcgacgat gatccggatt ttcaccgctt gggccgcccc ccagccgggg
     7981 ccggccagga aaacgatcat tcccaaaatc gcgccgacaa atggacgcct catgttgatt
     8041 gctccggaat gtttcgcggt ccgcatttta tcataaaacc cggtccgggc ggctttttcc
     8101 gatctccggg gtgaagcctc ctcccgtttg aattataatg gaataaaatc cggatccaaa
     8161 aggtattccg aatgaagatc cgcgaatgcc gggcctccgg tgcaaggaga gatcatgaag
     8221 ccgtcgctga aaaaagcggt tcccgccgtt ctgctcctgt ccttcctgtc gaccgtcccc
     8281 ggaagcgccg ttcggctaaa gccgggtcag gacaagccga aggtttcgac cgcggattta
     8341 tggaagctgg tcgaggccgc cgaccgtgac ggcctccccc ggacggccat cgagcacctg
     8401 aaggagatcg tccggctgtc cctcgaggag aagagggagg gcgaggctct ccgggccctg
     8461 acccgccgcc tggtcctgga gtcggtcgtc gagggcaaca agcccggggc ccgggtggcc
     8521 cggatccggg aggagatcga ccaggccccg gaggggatga aaacgatgct ccggaccatc
     8581 ctggcccaat ggtaccggca ttatttctcc cggaaccggt ggcggttcgc ccaacgctcg
     8641 gcgaccgccg gcggaatcga cgagaaggat ttcacgacct gggatctccg ccggatcgtc
     8701 cgggagatcg acgccctcta ccaggacatc ctgaaggatg aggcgtcttt gaaaagaacg
     8761 cccgtttcgg tctaccgcga ttttctcgag ccgggcgatc cgccggagtc cttgcggccg
     8821 acgatgttcg acttcgtcgc tttcgaggct ctcggcttct acgtgtcagg cgagcgggcc
     8881 gcggccgccc ccgaggacgc cttcgtcctc gaagccgatt cccccgtcct cggcccggcg
     8941 gacgaattca tcctttaccg gccggagacg accgacgagg attcgccggc gctccgggcg
     9001 cttcgcctct atcaaagcct tttggaattc caccgcggcg accgggaccg ggacgtgttc
     9061 ctggacgccg atcttcatcg cttggaagcc atgcggaacc tggccgccgg cgagtccgcc
     9121 tcggcccggt acctggagcg gctgaaggaa atcgcggaga agaatccggc ctcggccttt
     9181 tccagcctgg ccgattatct ccgggcccgg gaattgaaaa accagggcga cctggtccag
     9241 gcgtacgccc tggccgaccg cggacgaaaa gcccacccgg cctccctggg cggacgcaac
     9301 tgcgacgccc tgatttccga catcgcctcc cgcgatttcg atgtccggaa cgaacgggtc
     9361 gtctcccccg ggacatcatc ggtcctggac gtccgctacc gcaacctgac cgcccttcat
     9421 ttccgggccg tccgggatga cttcgaggcg cggttggcgg ccgaaaacgc gggggatctt
     9481 ttttggccgg gtgacgagcc cgtccgcgag cttctcgagc gggagccggc ggccgcctgg
     9541 tcggccgatc tcccggcgac ggccgacttc caggcgcgcg ccgagcgggt cgcggctccg
     9601 gccctgcccc cgggatttta tcgcctttta gtcagcgccg atccggcttt ttcccgggga
     9661 aagaacaaga tccaagccgg ctcgttctgg gtgagcgaat gggggattgt gacggccggg
     9721 gcgggacggg ccttggaggg attcgtcgtc cggggggtga cgggcgatcc cgtttccggc
     9781 gcgtccgtct ccctttatga ttgggattac ggaacggaca ggctggtcaa acgaggcgag
     9841 ggccggaccg acgacgccgg cgccttcgcc ctcgccgcgc ccgaatctta ttccaaccgt
     9901 cttttggtcg cccgggggga gaaggggatc gaagtcgttg aaacccaggt tttaacgggg
     9961 tccggccggc cggcctctta cgaaagggaa cggacggtct tcttcaccga ccgctcgctc
    10021 taccgtcctg gacagacgat ccacttcaag ggtttgtgcc tgaaggcgga cgagaaccga
    10081 gccgattacc ggatcctgcc ccggcggaac gtccgggtgg ttctccggga cgccaatgcc
    10141 caggaagcct ccgtcctgac tctcacgacc aacgatttcg ggtcgttcag cggggccttc
    10201 accgccccgg ccgaccgcct gacgggggcg atgacgattt cgaccgagaa tccccgggga
    10261 tcctgcccca tccgggtcga ggaatacaag cgtccgaagt ttcaagtgaa aatcgacgtt
    10321 ccggacaggg agttccgtct gaatgacgag atctccgtgc cggccgaggc gatgtcctac
    10381 gcgggggcgc cggtcgacgg agccttggtg aaataccgcg tgatccggga ggtccgctat
    10441 cccgtctggt ggtggtggac cacgccggcc cgcggggaat cccgggaaat cgcccacggg
    10501 acgtcccgaa cggacgctga aggaaagttc atggtcgttt tcaaggccgt tcccgatccg
    10561 aagtcctccc cctcctccca gcccgttttc acgtttaccg tcacggccga cgtgaccgac
    10621 ggggcggggg aaacgcggag cgcggcggga accatccggg ccgggtacac ggccctcgag
    10681 gccggtttgg ccggctcgga ctggcaggaa aagggcgagc cggtcgtctt gaccgcgacg
    10741 gcggcgacgc tgaacggcaa gccggccgcg gccgagggcg tcgtcgaagt ctacgccctc
    10801 aggggaccgg gccgtcccgt cccgcgggat ctgttcgctg aaagcgggga ccggggccgg
    10861 gttcggccct ccgggagcgc cgcctccggg gattccgaga cgatggattg gcgccggtgg
    10921 ccggaaggac gcttggccgg acggaaggaa ttcgccgtct ccgcggtcga gggttctccc
    10981 tgccggctgt cctgggatct tccgccggga gcctaccggg cgatcctcaa aaccgcggat
    11041 cgttacggaa cggcggtcga aagccgcttc gccttcctcg tcctcgatcc ggccgccgat
    11101 cgattcgcgg tccccgtccc gtttcatgcc gccgcccgct cgggctcggt cgagccggac
    11161 gggacgttcg agctgttttg ggggacgggg tacgagcgcg gtccggtctt gatcgaggtc
    11221 caccgggacg gccggcgcct gcaacgttat tggacgcggg ggaccgcgac ccaagccgtc
    11281 gtccgcgttc cggtcacgga ggccttccgg ggcggtttcg tcgtctcggc ggcgatcgtc
    11341 aaggacaacc gcttgtaccg ggaagaccgc cgggtttccg tgccctggac gaacaagcgg
    11401 ctcgaccttc ggtggaaaac gtt
//
LOCUS       DAOOXH010000079        11222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_6178_length_11222_cov_7.463509, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000079 DAOOXH010000000
VERSION     DAOOXH010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 11222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11222
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6178_length_11222_cov_7.463509"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..1487
                     /gene="rocD"
                     /locus_tag="PKV64_08955"
     CDS             279..1487
                     /gene="rocD"
                     /locus_tag="PKV64_08955"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HPN17242.1"
                     /translation="MNTKQYIAVEDKYGAHNYHPLDVVINKAKGIWVWDVDGRKYLDF
                     LSAYSAVNHGHNHPRIIRALVAQARELSLTSRAFRNDQWPLLAQELCRMAGYDMVLPM
                     NSGAEAVETAVKTARKWGYEKKGIPEDKAEIIVCANNFHGRTVTVISFSTEPLYRKNF
                     GPFTPGFTVIPFGDAAALEKAITPRTAAFLVEPIQAEAGILIPPDGYFKAAREICTKN
                     NVLFIADEIQTGLGRTGKLFAWEHEKAKPDMIVIGKSLGGGCYPISACLTSAAIMSVY
                     TPGTHGSTFGGNPLACAVARESLRVIKEEKLVANAVKMGKYFMAKLRTIKSKNIKEVR
                     GKGLLIGVELFPEAGGARRYCEALAADGLLCKETHDNVIRFAPPLIIGTKDLDWAFAR
                     IKSVFDRLEN"
     gene            1558..2808
                     /locus_tag="PKV64_08960"
     CDS             1558..2808
                     /locus_tag="PKV64_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPN17243.1"
                     /translation="MKRSCPYRSMPSENVIERRDFLKRGAAVAAAAAAVASSGLGSRY
                     LFAHESGQAASAVKTPPSRPKTNIEDALKIPRTKFSLPGLRPGRVVEVHDPAAMPAGK
                     PDPAAVRAMFENGLAALTGKNPAESFELFFSKDDIVGLKVNPVGAGLISTRLELVDAV
                     AAWLEAGGIARKNIVIWDRFDFMLTDAGFTAGRFPGMSIEGLQTMDEQAQEGTTADNS
                     KWLKPDGTHVSVPNFDLDRYYWADVDGPKDLGYLNQHVFNGKYSYFGKLLTKRLTKII
                     NLPVFKNTGNAASMATKNIGYGAVCNTNRLHAPLFLDVCVEVLAFPDVRDKMVLNVTD
                     GLLCQYDGGPGPNAKFTYIDNRIYLATDPFALDMVCHNLLAAKRKEMGVAVNENPRFT
                     EYLRYAERLGLGVVAAGKISHLKI"
     gene            complement(2816..5686)
                     /locus_tag="PKV64_08965"
     CDS             complement(2816..5686)
                     /locus_tag="PKV64_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPN17244.1"
                     /translation="MNEAGRRKIRDPRGTPFPEKRLAASSDGKTKRLAALLSLGYLVL
                     AAAILYVGRASYLLYLKKLEIKVQDELVSIADLKTEQLARWRAERLADAFFLDENVFI
                     SVLAGRVLKTPDDDRSRRQLAAWVEKFEASGIYDRIQLLDREGAAFLSVPAGLPGVSA
                     AVKRRLPDIFFSGKPEIVDFFMDESEGQPFLSLLIPIFKNGIRPDVESVLALRLDPRM
                     YLYPYLARRSSSSRTAETHLVRRDGDDAVFLDEDRFRQNNPFPVRIPLRDNTIFGQAV
                     EGREGLIRGIDDRRNPVIACLRRVPGSPWFLVAQIDADELYAPARKRFLGQMAVIISI
                     LTAVGLAAAMIPRRQKMRFLRERLAAARSLHELSSRQEALIASLPEIIIEIDPAKVCF
                     WANMSGLDFFGEDVIGKNIGRYYASDEDLQTKMSPLFEGSGEAVHIEGRHRRRDGEIR
                     LLGWWCRGIKDPEGRVTAVLCSGRDITEERRDAEDRRESEARYRTLVDNAPAAVFVVR
                     EDRVVLVNAACTRLFGAAAPDLLLDRSPYDLIHPDHREAFRERIRRLRDAGEVFPTLE
                     KKIVRLDGSTRDVEVTAVPFPDRGVMAVHVVLSDITERKRVEAERETALAAVKENQSI
                     FDQFMKNSPVYMFFKDENIRALSLSANYSIMLGMPVTAALGKTMSELFPSDLARNMVA
                     DDQRILREGRTITVDEELNGRHYTTIKFPIHIEGKPRYLAGFTLDITERKLAEDRIKA
                     ANEALLVSLREKELLLQEVHHRVKNNMQIISSLFNLESEHASSETREILKRGQARIRS
                     MSLIHEKLYRSVDLSKIDLSGYLESLSTHLFQIYVVDPRQIRLETDIAEVRLDINSAI
                     PFGLIINELISNALKHAFPGGRKGVIQIRLARAAGDTVILQVSDDGVGLPEGFDRSNS
                     AGFGFQIINLLVEQLDAVLDVDRNDGTTFTLKFKELKYPSRP"
     gene            5840..8074
                     /locus_tag="PKV64_08970"
     CDS             5840..8074
                     /locus_tag="PKV64_08970"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HPN17245.1"
                     /translation="MTLTTRNGHKYGAPRAVSLFLAVLSFAVSLSAADGIRAVRVAAG
                     PEINGRLDDPAWAAAPAVSAFRMVEPTPNADPSERTELRIVYDENALYIGVLCFDGEP
                     NRIVGNTMAHDNTGASSSGGWSGGGYGHGSTAPGDDLVRILLDPFQDKRTAYVFFVNP
                     RGARGEGLVYAGSASLNWDGIWTAAARIREDGWSAEFRIPFKTISFKPGLSVWGLNVE
                     RYIPRKQETIRLAGVSRDANFYNPREAASLEGIENIRQGKGITFRPYGLTGIAKSFTA
                     GTGASFRLDGGFDVYKSFTPNLVGVLSYNMDFAETEADERRLNLTRFPMSYPEKRMFF
                     LEGSETFSFSSSMSFTPFFSRAVGLVNGVPVPVLFGAKLYGKIGDTNLALLDVQTRAD
                     EGRPGLNFLAARMTQNIFAQSKIGWIFTNGSPSGEKNSLFGADITFSSSTFLGNKNIM
                     LAAWGAYNWTERKDGRHHGFGFRADYPNDLWNIQTTYAYYGDALNPGIGFMMRRGIQT
                     AFGRLSFQPRPAAGFLGALVRQFFFTASADYYWDLAGNLETRRLTASPFGFQTESGER
                     ITFEIESNRDVLPYDFEISRGIVLPAGAYDFTKTRLEFSTSARRSVAVSVHYNFGPFY
                     SGRYNDFNAGLTVKVDGFVNLSFSTNLVRGFLAQGDFSQNVYEVKADFFLSPDLGLMN
                     YIQYDDVSRLLGWSSRLRWRISPGNEIYVIYNKNWERRWDPASRFLPSEERGVLKLSL
                     SIRP"
     gene            8143..9081
                     /locus_tag="PKV64_08975"
     CDS             8143..9081
                     /locus_tag="PKV64_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17246.1"
                     /translation="MKSVCRRFLFRAIPLALLAGAAAAQDILPPGGFVRGWEAAGRPR
                     SFIEKDLFNHINGGAELFLEFGFARLLVQPYAGGGKDELVLEVYEMTEPAAALGIYLM
                     SAGRETPWPEIPARNSSEEAQIVAVKGRYFLKVEHYDSGPGLRPAMIALARNVLSRIP
                     DVPLENPFAALAEDGRVAGSERLIRGPVGLQPYYSFGEGDILGLEGRTFAFLADYRDG
                     DDPPFTRLVADYPSPEAADGVLRNLRAHLDPYLKIIAECPGGGFDFSDFQGKYGRVER
                     AEARLEITFKRASLDRRPPASSFSGSTGKGRKPPSR"
     gene            complement(8996..9886)
                     /locus_tag="PKV64_08980"
     CDS             complement(8996..9886)
                     /locus_tag="PKV64_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPN17247.1"
                     /translation="MSRSRSLLAVHAAVALFGLAGLFGKWLTLRPHFIVLGRVVFAAA
                     ALALIIRLSGGRFSIRRKIDGGLFLLQGLILAVHWILFFESIRVSTVAVGLLAYSSFP
                     VFTAFLEPLFFRTRLSRRHLGLSLACLAGVFLLVPRFSWSERVFQGVVTGLGAGATFA
                     LLSIINRKLSFRYASPVVAFYQDAGAALFLLPAALLKPPALTGRDFALLAILGLLCTA
                     LAHTLFIAGMRRLSAQTASIISALEPVYGIALAALLLGERPSARTIAGGLIILTAVFV
                     LSLSNLKRKKPAAAGRATPA"
     gene            9997..>11222
                     /gene="dnaX"
                     /locus_tag="PKV64_08985"
     CDS             9997..>11222
                     /gene="dnaX"
                     /locus_tag="PKV64_08985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPN17248.1"
                     /translation="MSTPHSTVLARKYRPMTFEEMVGQRAVVQTLQNAVRTGRVAQAY
                     LFSGTRGVGKTTAARILAKALNCEQGPTPTPCGICESCRAVQEDRQLDVLEIDGASNT
                     GIDNIRDLRDLVRYKAMKSRYKVVIIDEVHQISKPAFNALLKTLEEPPEKTLFIFATT
                     ELQKVPATIISRCQHFEFKRVSPKDLFNHLHDVARKEGLELTENGFALLARAAEGSVR
                     DSLTLLDQAVAFCGRTIADADLDLILGTINRDLLFEFSSAVIAGAPDRIFPLVEKVVD
                     SGYDLRTFHKRLIEHFRDLLIVRTMDKPQDILHLGEADLAFFRTEAEKTGGEELLRFL
                     QALQDGEAGLRYSSQPQIAFETILVRLCHFGKLVELKDVLAGLEGGSAGTALRPSSAT
                     VRPAAIRPQAAVPAAPA"
BASE COUNT         2159 a         3506 c         3500 g         2057 t
ORIGIN      
        1 tccttcccta gtcataggga aggcaggatg ggtaggttca tataggagga acgcctgcct
       61 gacgtcctcc ccgagtcata ggggaggaag gagggtaggt acatgcaaga aggcagccgg
      121 aggagtagag aattgatgat tgagtcttgg gaggcggccc ggttttaagg atcatgacga
      181 gaaccgatga acgttctatc gaaaagcaac aataatttga agccgtttcg gtcttctgaa
      241 ttcaatgtcc gccgccgagc gggccaaagg agtttcacgt gaacacaaaa cagtacatcg
      301 ccgtcgaaga caagtacggc gcccacaatt accatcccct tgacgtcgtc atcaacaagg
      361 ccaagggcat ctgggtttgg gatgtggacg gccgcaaata tctcgatttc ctcagcgcct
      421 actcagccgt caaccacggc cacaaccacc cccgcatcat aagggccttg gtcgcccaag
      481 cccgcgagct gagcctgacc tcccgggctt tccgcaacga ccagtggccg ctgttggccc
      541 aggaactgtg ccggatggcc ggctacgaca tggttctgcc gatgaattcc ggggccgagg
      601 ctgtcgagac ggcggtcaag accgcccgga agtggggcta tgagaagaaa ggcatccccg
      661 aggacaaggc cgagatcatc gtctgcgcca ataacttcca cggccggacg gtcacggtca
      721 tcagcttctc gaccgagccg ctttaccgga agaatttcgg acccttcacc ccgggcttta
      781 ccgtcattcc tttcggcgac gcggccgccc tcgaaaaggc gatcacgccc cgcaccgccg
      841 ccttcctcgt cgagcccatc caggccgagg ccggcatcct gatccccccg gacggatatt
      901 tcaaggccgc ccgggaaatt tgcacaaaga acaacgtcct gttcatcgcc gacgagatcc
      961 agaccggcct gggccggacg ggcaagctgt tcgcttggga gcatgaaaag gccaagcccg
     1021 acatgatcgt catcggcaaa tccctcggcg gcggatgtta tccgatctcc gcctgcctga
     1081 cgagcgcggc catcatgagc gtctatacgc ccggcaccca cggctcgacg ttcggcggca
     1141 accccctggc ctgcgccgtc gcccgcgaat ccctcagggt gatcaaggag gagaagctcg
     1201 tcgccaacgc ggtcaagatg ggcaagtatt tcatggccaa gctgcggacg atcaagagca
     1261 aaaacatcaa ggaagtccgg ggcaagggcc ttttgatcgg cgtggagctt ttccccgagg
     1321 ccggcggcgc ccgccggtac tgcgaggccc tggccgccga cgggctcctc tgcaaggaaa
     1381 cccacgacaa cgtcatccgt ttcgccccgc ccctgatcat cggcacgaag gatctcgact
     1441 gggcgttcgc ccgcatcaag agcgtcttcg accggctcga aaactgatcc gccggaccgg
     1501 cgttcccggg aatgtcccgg ggacgccggc cgctttcccg ccggccgagg aggagccatg
     1561 aagcgttcgt gtccttaccg ttcgatgccc tctgaaaatg taatcgagcg ccgcgatttc
     1621 ctcaagcgcg gcgcggccgt cgcggccgcg gccgcggcgg tcgcctcgtc cgggctggga
     1681 agccgatatc tcttcgccca cgaatcaggt caagcggctt cggccgtcaa gacgccgcct
     1741 tcccggccga aaaccaacat cgaggatgcc ctgaaaattc cccgaaccaa gttctccctt
     1801 ccgggactcc gccccggccg ggtggttgag gttcacgatc cggcggcgat gccggcgggg
     1861 aaacccgatc cggcggccgt ccgggccatg ttcgagaacg ggctcgccgc cctgaccgga
     1921 aaaaatccgg ccgaaagctt tgaactcttt ttttccaagg acgacatcgt cggtctcaag
     1981 gtcaatccgg tcggggcggg cttgatcagc acccggctgg agctggttga tgccgtcgcc
     2041 gcctggctcg aggccggcgg catcgcccgg aaaaacatcg tcatctggga ccggttcgat
     2101 ttcatgctga ccgacgccgg cttcacggcc ggtcggtttc ccgggatgtc gatcgagggg
     2161 cttcagacga tggacgagca ggcccaggag ggaacgacgg cggacaacag caagtggttg
     2221 aagcccgacg gcactcacgt cagcgtcccg aatttcgatc tcgaccggta ttattgggcc
     2281 gatgtcgacg gtcccaagga cctcggctat ctcaatcagc atgtcttcaa cggcaaatat
     2341 tcttacttcg ggaagcttct gaccaagaga ctgaccaaga tcatcaacct cccggttttc
     2401 aagaacaccg gcaacgcggc ttcgatggcc accaagaaca tcggctacgg ggcggtctgc
     2461 aacaccaacc gcctccatgc cccgctgttc ctcgacgtct gtgtcgaggt cctggcattc
     2521 cccgacgtcc gggacaagat ggtcctcaat gtcaccgacg ggctcttgtg ccagtacgac
     2581 ggcggcccgg ggccgaacgc caaattcacg tatatcgaca accggattta cctggccacg
     2641 gaccccttcg ccctggacat ggtctgccac aacctcctgg cggccaagcg gaaggaaatg
     2701 ggcgtggcgg tcaacgagaa tccgcggttc accgagtacc tgcgctatgc cgagcggctg
     2761 ggattgggcg tggtcgccgc gggcaaaatc agccacctga agatttgagc cgggctcagg
     2821 gccgggacgg gtatttcagc tccttgaact tcaaggtgaa ggtcgtcccg tcgttgcggt
     2881 cgacgtccaa gacggcgtcc agctgttcga cgagcaggtt gatgatctga aatccgaagc
     2941 cggcggagtt gctccggtcg aagccctccg gcagcccgac gccgtcatcc gacacctgca
     3001 ggatcaccgt atctccggcg gcccgggcga gccggatctg aatgacgccc tttcggccgc
     3061 ccgggaaggc gtgtttcaag gcgttggaaa tcagctcgtt gatgatcagg ccgaagggaa
     3121 tggccgagtt gatgtccagc cgaacttcgg cgatatccgt ctccaggcgg atctggcggg
     3181 gatcgaccac gtagatctgg aacaggtggg tggacaggct ctcgaggtat ccggacaggt
     3241 cgatttttga aagatcgacc gaccggtaga gcttctcgtg gatcaggctc atcgaccgga
     3301 tgcgcgcctg tccccgcttg aggatctcgc gggtttccga agacgcgtgc tcggattcca
     3361 ggttgaacag gctggagatg atctgcatgt tgttcttgac ccggtggtgg acctcttgga
     3421 ggaggagctc tttttcccgc agagagacga gcagggcttc gttggccgcc ttgatccggt
     3481 cctcggccag cttccgctcg gtgatatcga gagtgaatcc ggccagatac cggggcttgc
     3541 cttcgatatg gatcggaaac ttgatcgtcg tgtaatgccg cccgttcaat tcctcgtcaa
     3601 cggtgatcgt ccggccttcc cgcaggatcc gctggtcgtc ggcgaccatg tttcgggcca
     3661 agtcggacgg gaaaagctcg gacatggtct tgccgagcgc cgcggtgacg ggcatgccga
     3721 gcatgattga ataattcgcg ctcaagctga gggcccggat gttctcgtcc ttgaagaaca
     3781 tgtacaccgg gctgtttttc atgaactggt cgaaaatcga ctgattttcc ttcacggccg
     3841 ccaaggcggt ttcccgctcg gcttcgaccc gcttgcgctc ggtgatgtcg gacaggacca
     3901 cgtggacggc catgacgccg cggtcgggga agggaacggc cgtgacttca acgtcgcgcg
     3961 tggagccgtc caggcggacg atctttttct cgagcgtcgg gaaaacctct cccgcgtcgc
     4021 ggaggcggcg gatccgttca cgaaaggcct cccggtgatc agggtggatc aggtcgtaag
     4081 gggatctgtc cagcagcagg tccggagcgg cggctccaaa cagccgcgtg caggcggcgt
     4141 tcaccaggac gacccggtct tcgcggacga cgaagaccgc cgccggggcg ttgtcgacca
     4201 gcgtccggta gcgggcttcg ctttcccgcc ggtcttccgc gtcccgccgt tcctcggtga
     4261 tgtcccggcc ggagcaaagg acggcggtca cccggccctc gggatccttg atcccccggc
     4321 accaccagcc gagaagccgg atctccccgt cccggcggcg atgacggccc tcgatgtgaa
     4381 ccgcctcgcc ggagccctcg aagagggggg acattttcgt ctgcaggtcc tcatcgcttg
     4441 cgtaataccg gccgatgttc ttgccgatca cgtcttcccc gaagaagtcc aggcccgaca
     4501 tgttggccca gaagcagacc ttcgccggat caatttcgat gatgatctcg ggaagagagg
     4561 cgatcagggc ttcctggcgc gaggacagct cgtggagaga ccgggcggcg gccaggcgct
     4621 cgcggaggaa gcgcatcttc tgccgccggg gaatcatcgc cgcggccagc ccgacggcgg
     4681 tcagaatgga gatgatgacc gccatttgtc ccaggaaccg tttccgggcc ggggcgtaga
     4741 gctcatcggc gtcgatttgg gcgaccagga accagggaga gccgggaacg cggcggaggc
     4801 aggcaatcac cggattccgg cggtcgtcga ttccccggat gaggccctcc cgcccctcga
     4861 cggcctgtcc gaaaatcgta ttgtcccgga ggggaattct gacgggaaag gggttgtttt
     4921 gccggaagcg gtcctcgtcg aggaaaaccg catcgtcgcc gtcccggcgg acgagatgag
     4981 tttcggccgt tcggctggac gaggaccggc gggcgaggta gggatagagg tacatcctcg
     5041 gatccaggcg gagagcgagc acgctttcga cgtccggacg gatgccgttc ttgaaaatcg
     5101 gaatcaggag gctgaggaac ggctggcctt cggattcgtc catgaaaaaa tcgacgatct
     5161 cgggctttcc cgagaaaaaa atgtccggga gccgccgttt gacggcggcc gagacgccgg
     5221 gaaggccggc cgggaccgag agaaaagccg ctccctcgcg gtcgaggagc tggatccggt
     5281 catagatgcc gctggcctcg aatttttcca cccaagcggc gagctgccgg cgggaacgat
     5341 cgtcgtcggg cgttttgagc actcgtccgg ccaggaccga gataaagacg ttttcgtcca
     5401 ggaaaaacgc gtcggccagg cgttccgccc gccatcgggc caattgctcg gtcttcaggt
     5461 cggcgatcga aaccagctcg tcttggactt tgatttcgag tttttttaaa taaaggaggt
     5521 aggacgcgcg tcccacgtaa agaatcgcgg ccgcgaggac gaggtatccc aaggagagaa
     5581 gggcggccag ccgtttcgtt ttcccgtccg aggaagcggc caagcgtttt tccgggaacg
     5641 gcgtcccccg cgggtcgcgg attttccgcc ggccggcttc gttcacgtgc tctcctcggc
     5701 gcccgttcga tcaggggctg ggaaaaaaga ataaagaata ttcgggtccg gccggcatgt
     5761 caatatctct tgggggccgc ccaacaatcc ccttgcggtc tttcctcgat gcggttatca
     5821 taaccgtctt tatatcctca tgacgttgac aacgcgaaac ggccataaat acggagcgcc
     5881 ccgagccgtc tcccttttcc tcgccgtcct gtccttcgcc gtctcccttt cggccgccga
     5941 cgggattcgg gccgtccgcg tcgccgccgg gcccgaaatc aacggccgtt tggatgatcc
     6001 cgcttgggcc gccgcgccgg ccgtgtccgc ttttcgcatg gtcgagccga cgccgaacgc
     6061 tgatccgtcg gaaaggaccg agctgcggat cgtctatgac gagaacgcgc tgtatatcgg
     6121 tgtcctgtgt ttcgatggag agccgaaccg gatcgtcggc aataccatgg ctcacgacaa
     6181 taccggggcg tcctcctcgg gagggtggtc cggcggcggt tacgggcatg gctccaccgc
     6241 cccgggtgac gatctggtca ggattcttct cgatccgttc caggacaaac gcacggcgta
     6301 tgtctttttc gtcaatcccc gaggagcccg aggcgaggga ctggtctatg ccggaagcgc
     6361 cagcctgaac tgggacggga tttggaccgc ggccgcccgc atccgggagg acggctggag
     6421 cgccgaattc cgcattccct ttaaaacgat ctccttcaag ccgggccttt cggtctgggg
     6481 gctcaacgtc gaacggtata tcccgcgcaa gcaggagacg atccgactgg ccggcgtctc
     6541 ccgggacgcc aatttctaca atccgcggga ggcggcgtcc ctggaaggca ttgagaacat
     6601 ccgccagggc aagggaatca ccttccggcc ctacggcctg accggaatcg ccaagagctt
     6661 taccgccggg acgggcgcct ccttccggct ggacggcgga ttcgacgttt acaagagttt
     6721 cacgcccaac ctggtcggcg tgctcagcta caacatggat ttcgccgaga ccgaggccga
     6781 cgagcgccgc ctcaacctga cgcgatttcc gatgtcctac cccgagaagc ggatgttttt
     6841 cctggaggga tcggagacgt tcagcttcag ctcgagcatg agcttcactc ccttcttcag
     6901 ccgggccgtc gggctggtca acggcgtgcc ggtcccggtt ctcttcgggg ccaagctcta
     6961 cggcaagatc ggagatacca acctggccct gctcgatgtc caaacccggg cggacgaagg
     7021 ccggcccggc ctcaacttcc tggccgcccg gatgacccag aacattttcg cccaaagcaa
     7081 gatcggctgg attttcacca acggcagtcc ctcgggcgag aaaaattcat tgttcggggc
     7141 ggacatcacc ttcagctcat ccacgtttct tggaaacaag aacatcatgc tggccgcctg
     7201 gggagcctac aactggaccg agaggaagga cggccggcat cacggcttcg gcttccgggc
     7261 cgactatccc aacgatctct ggaacatcca gacgacctac gcgtactacg gagacgcctt
     7321 gaatcccggc atcgggttca tgatgcgccg cggcattcaa accgccttcg gacgtctttc
     7381 gttccagccg aggccggccg cgggattcct cggggctctc gtccggcaat tcttcttcac
     7441 cgccagcgcc gattattatt gggacttggc cgggaacctc gaaacgcgcc ggctgaccgc
     7501 ttcgccgttc gggttccaga cagaaagcgg cgagcggatc acgttcgaaa tcgaatcgaa
     7561 ccgcgatgtc ctgccctatg atttcgagat ttccagggga atcgtccttc cggccggggc
     7621 ttatgatttc accaagacca ggctcgaatt ctccacgtcg gcccgccgct ccgtggctgt
     7681 ctcggtccat tacaatttcg gcccgttcta ttcgggccgc tacaacgact tcaacgccgg
     7741 cttgacggtc aaggtcgacg ggttcgtcaa cctctccttc agcaccaacc tcgtccgcgg
     7801 tttcctggct cagggcgact tcagccagaa cgtttacgag gtcaaggccg acttttttct
     7861 ctcccccgac ctcggcttga tgaattacat ccaatacgat gatgtttccc gcctgctggg
     7921 atggagcagc cgcctgcggt ggaggatttc cccgggaaac gaaatctacg tgatatacaa
     7981 caagaactgg gagcgacgct gggatccggc ctcgcgtttt ttgccgtccg aggagcgggg
     8041 cgtcctgaag ctttcgctct cgatccggcc gtaaggccgg acccggcttt tccaaaggtc
     8101 cgcccgctgg tacaatggtc ccggccgccg ccggaggttt tcatgaaatc cgtttgccgc
     8161 cgttttcttt tcagggcgat tcccttggct cttctggccg gagccgccgc ggcccaggac
     8221 atccttcccc ccggcggatt tgtccgcggc tgggaagcgg ccggccgtcc gcgctccttc
     8281 atcgaaaaag acttgttcaa ccatatcaac ggcggggcgg agcttttcct ggaattcggc
     8341 ttcgcccggc ttctcgtcca gccctatgcc ggcgggggaa aggacgagct cgttctcgag
     8401 gtgtacgaaa tgaccgaacc ggcggccgcc ctgggcattt acctgatgag cgccgggcgg
     8461 gaaacgccgt ggccggagat cccggcccgc aattcgagcg aggaggcgca gatcgtcgcg
     8521 gtcaagggga ggtatttcct caaggtcgaa cattacgatt cgggcccggg tcttcgtccg
     8581 gcgatgatcg ccttggcccg gaatgtcttg tcccgcatcc cggacgttcc gctggagaat
     8641 ccgttcgccg ccctcgcgga agacggccgt gtcgccggaa gcgagcgctt gatccggggt
     8701 ccggtcggcc tccagccgta ctattccttc ggggaagggg atatcctcgg gttggagggg
     8761 aggacgttcg ccttcctggc cgattaccgg gacggcgatg atccgccgtt cacccgtctt
     8821 gtcgcggatt acccctcgcc ggaggcggcc gatggagtcc tgcggaacct ccgggcccat
     8881 ctcgatccct atctcaagat catcgccgag tgtccgggcg ggggattcga tttctccgat
     8941 ttccaaggga aatacggacg ggtggagcgg gcggaggccc gtcttgagat tacgttcaag
     9001 cgggcgtcgc tcgaccggcg gccgccggct tcttcctttt caggttcgac agggaaagga
     9061 cgaaaaccgc cgtcaagatg atcaatccgc cggcgatcgt ccgggcggaa ggccgttccc
     9121 caagcagcag ggcggccaaa gcgatcccgt aaaccggctc caaggcgctg atgatgctgg
     9181 ccgtctgggc cgaaaggcgc cgcatcccgg cgataaaaag cgtgtgggcg agggcggtgc
     9241 acagaagccc gaggatcgcc agcagggcga agtcgcgtcc cgtaagagcc ggcggcttga
     9301 gcaaggcggc ggggaggaga aaaagagccg ccccggcgtc ctggtagaac gcgacgacgg
     9361 ggctggcgta ccggaatgac agcttccggt tgatgatgct cagcagggcg aaggtcgccc
     9421 cggcgccgag gccggtcaca acgccttgaa aaacgcgctc ggaccaggag aagcgcggaa
     9481 cgaggaggaa cacgccggcc aggcaggcca gggacaggcc gaggtgacgc cggctgaggc
     9541 gggtccggaa aaacaacggc tcgaggaagg ccgtgaagac cgggaagctc gaataagcca
     9601 gcagccccac ggccacggtc gagacgcgga tcgactcgaa aaagaggatc caatgaacag
     9661 ccaggatcaa gccctgaagg agaaagagcc cgccgtcgat tttgcgacgg atcgaaaacc
     9721 ggcctccgga aaggcggatg atcaaggcca gggccgcggc ggcgaaaacg acccgtccga
     9781 ggacgatgaa gtgcggccga agggtcagcc acttgccgaa aagaccggcc aacccgaaca
     9841 gcgcgacggc cgcatggacg gccaacaggc ttcgggatcg gctcacggcg ccccgattat
     9901 aacaaaaatc ccctttcccg gaagacccgg tttgtggtaa attaatccgt actcttcggg
     9961 agattgatcc tcccggcggt acacccggaa aacagcatgt ccacaccgca ttcgaccgtc
    10021 ttagcccgca aataccggcc catgaccttt gaggaaatgg tcggtcaaag agccgttgtc
    10081 cagactcttc aaaacgccgt ccggaccggc cgcgtcgctc aggcctacct cttttcgggg
    10141 acgaggggag tgggcaaaac gaccgccgcc cgcatcctgg ccaaggccct caactgcgaa
    10201 cagggcccga cgccgacgcc ctgcggcatc tgcgaatcct gccgggccgt ccaggaggac
    10261 cgccagctgg atgtcctgga gatcgacggg gcgtccaaca cgggcatcga caacatccgg
    10321 gacttgcgcg accttgtccg gtacaaagcg atgaagagcc ggtacaaggt cgtcatcatc
    10381 gacgaggtcc accaaatctc caagccggcg ttcaacgccc tgctgaaaac gcttgaggag
    10441 cccccggaaa agacgctgtt catttttgcc accaccgagc tgcaaaaagt accggcgacc
    10501 atcatctccc gctgccagca tttcgagttc aagcgcgtct ccccgaaaga cttgttcaat
    10561 catcttcacg atgtcgcccg gaaggaaggg ctggagctca ctgaaaacgg cttcgccctg
    10621 ctcgcccggg cggccgaggg aagcgtccgc gacagcctga cgctgctcga ccaggcggtg
    10681 gccttctgcg gccggaccat cgccgacgcc gacctcgacc tgatcctggg aacgatcaac
    10741 cgcgatctct tgttcgaatt ctcctcggcc gtgatcgccg gcgccccgga ccggattttt
    10801 ccgctcgtcg agaaagtcgt cgattccggc tacgacctcc ggacgtttca taagcggttg
    10861 atcgagcatt tccgcgatct cttgatcgtc cggacgatgg ataagcccca ggacatcctc
    10921 catctgggcg aagcggacct ggccttcttc cggaccgagg cggagaaaac cggcggggaa
    10981 gagcttctcc ggttcctcca ggccctccag gacggggaag ccggcctccg atattcctcc
    11041 cagccgcaga tcgctttcga aacgatcctg gtccgtctct gtcatttcgg gaagctggtc
    11101 gagctgaagg acgttttggc cggtctcgag gggggaagcg ccgggacggc tcttcggccg
    11161 tcgtccgcga ccgtccggcc ggcggcgatc cggcctcagg cggcggtgcc ggcggctccg
    11221 gc
//
LOCUS       DAOOXH010000080        11040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_6334_length_11040_cov_7.169595, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000080 DAOOXH010000000
VERSION     DAOOXH010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 11040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11040
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6334_length_11040_cov_7.169595"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PKV64_08990"
     CDS             complement(<1..774)
                     /locus_tag="PKV64_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPN17249.1"
                     /translation="MHIDDLLKIAIEMEASDLHLKAGTHPQVRVAGQLVVLEKIPRLS
                     IDDTENLAAEMMGESQRDRLKEELDIDLAYSLPGFGRFRGSIYYQRSSVAIALRIIHF
                     EIKTLKELLLPEILEKIANYTRGLVLVTGTTGSGKTTTLASMIDHINGLRRENIITIE
                     DPIEYLHRDRKSSISQREVGWDVKSFARGLRAALREDPDIILVGEMRDQETIETALTA
                     AETGHLVLSTLHTLDAPETVNRIVGVFPPHQQRQIRNQLA"
     gene            complement(876..1814)
                     /locus_tag="PKV64_08995"
     CDS             complement(876..1814)
                     /locus_tag="PKV64_08995"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPN17250.1"
                     /translation="MFLQGQKIGKYEIIRSLGSGGFGSVYLAKDIWLDIKVAIKVPHK
                     QSIELYKLLKEPRLQAVLDHPNIVRLLAAEKIDKIFFFVMEYVRGRTLEKILQKEKVL
                     ETAAAIDMMKQLAAGVDHAHKNKIIHRDLRPSNIIVSEEGQVKITDFGTSAWLQDVPY
                     ATTRIGSPPYMAPEQFMGKATYASDIYSLGCILYEMMIGRPPILDPDPFKILERAQEG
                     RITPPRLKNTRVPREIDALIMRCLANRIEDRFQRPFEIIRALNEIKGEKPAKNEIDDI
                     LGRIRAREPATTTTCWNCRRHLPGRAKTCPYCGEAC"
     gene            complement(1818..2555)
                     /locus_tag="PKV64_09000"
     CDS             complement(1818..2555)
                     /locus_tag="PKV64_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HPN17251.1"
                     /translation="MTSEYFFLPRADVEGSSAVLRGAEYHHLARVLRVRPGGRVWLFD
                     EDGRRYRAEAAAVREKEEEIDLSILEVLPPRGVRTRIILAPALLKTGAMDEVILKACE
                     LGASRISPVESERSVARSGDHSRRKVERWTRLARAAAKQSRAARVPLIDAPASLADFL
                     ASGRDGRRIFLCEHGGMSLKSLRGGEEAPPAEATVLVGPEGGWSAREEEAIRAAEFEA
                     VSLGETILRAETAALAALAVMAHEWNW"
     gene            complement(2563..3678)
                     /gene="dnaJ"
                     /locus_tag="PKV64_09005"
     CDS             complement(2563..3678)
                     /gene="dnaJ"
                     /locus_tag="PKV64_09005"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPN17252.1"
                     /translation="MANEDYYEVLGVSRTASSEEIKKCYRQLAMKYHPDRNPGDKDCE
                     ERFKKAAEAYSVLGDPQKRETYDRFGPDGLRGPAFEGFSGSIFEEFEDILGNFFGFNF
                     GFGGTARRPRAARGRDLGLGVEITLEEAAAGVDKDITIQRAELCPSCGGSKARAGTRP
                     AVCPACGGRGQVRRSQGFFSIASPCGRCRGTGEVITDPCPDCRGGGRKTQKRTLKIRI
                     PAGVADGIRLRMTGEGEAGDPGSGRGDLYVDIRIKPHDIFEREENNLICGLSLSFSQA
                     ALGVTAEIPSLSGKTEKLKIPAGTQSGEIFRIKGCGLKDLSRHRTGDLFVKVDVQTPA
                     ELSREEKELLRRLAESRRENLDRLEAGAVRRASPGSR"
     gene            complement(3690..5255)
                     /gene="dnaK"
                     /locus_tag="PKV64_09010"
     CDS             complement(3690..5255)
                     /gene="dnaK"
                     /locus_tag="PKV64_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPN17253.1"
                     /translation="MSRVIGIDLGTTFSCVAHLSPEGPKVIPNLEGMATTPSMVCFTS
                     AGEILVGNLAARQALANPEKTIFAIKRLIGNKYSSPAVQEARPRLTYGLSEAPNGDVL
                     IRAGDRTITPQEASSHILVYLKRCAESFFGEPVTECVITVPAHFDDHQRQATKDAARI
                     AGLDVLRVINEPTAASLAYGLNRNPDGVVAVYDFGGGTFDITLLEISGGVFHVLATAG
                     DNFLGGDDFDQRIIDRLRADFLSKHGIDLGSNPYNLQRLKEAAERAKRDLSFTAITEV
                     NLPFIAADASGSKHLQTAITRANLEEWTRDLVERTLPLMDKALHESGLTANKVAEILL
                     VGGQSRMPLVRKKVGEFFGKDPNTQINPDESVALGAAIQSGVLEGNGAKDIVLLLDVT
                     SLSLGIETENGEFEKIIDKNTTIPCRKTKSFTTVDDNQRRVRIHVLQGEGSRALENTS
                     LAMFDLVGIMDAAAGVPRIDVTFEIDADGMVRVSARDVLSGREQKVSVHPSSGLSPQQ
                     VENIIEREKKKAH"
     gene            complement(5257..5958)
                     /locus_tag="PKV64_09015"
     CDS             complement(5257..5958)
                     /locus_tag="PKV64_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPN17254.1"
                     /translation="MINEDRKIQDEKDVLSAPSAEAAAPEGAIPAPPVEPDSAGRKKT
                     SEHRNHKNNKDHQKIKSLKAEIEALKKDLEQRTREAAAAREEVETARKESLEWKDRTL
                     RAMADTDNFRKRLEREKDEFFLFSLSDILKEFLQVIDNLERALDARDASDGLREGVEL
                     ICRQMLDLVGKRGVTLIERPDGRFDPNVHQALLTEEAEGIEEPMIGEELQKGYMLNDR
                     LLRPALVKVLMPKKN"
     gene            complement(5970..7016)
                     /gene="hrcA"
                     /locus_tag="PKV64_09020"
     CDS             complement(5970..7016)
                     /gene="hrcA"
                     /locus_tag="PKV64_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HPN17255.1"
                     /translation="MKNQELREKDRLVLAAAVELYLKTGLPVSSGAIHQKKVLTDSPA
                     TIRNILVRLGERGYLSQPHASSGRIPTDRGLRFYVNSLLAGVPLTFDRINLFAEDLSL
                     GGSDLPGLLHQASRVLADQSDSLGFVIQPRISRIHFRHIRFIQIAAGKVMIILMTPFQ
                     LVLTEIVPTDTTFDQVELDRASQYINASFSGRTLSQVRDCVLEELGKSTSKFERALSR
                     LTSLLKASIQQDDMEDPIIVQGASRLIGKFEKADLPRLKMLFQNIEEKASLAKLLSEF
                     IALDRVKVLIGSESNMPEISDCALVLSHYGDRTQVLGSLGIIGPKRIPYDKIIPLVDN
                     VAQKISRTISGASS"
     gene            complement(7124..7930)
                     /gene="amrB"
                     /locus_tag="PKV64_09025"
     CDS             complement(7124..7930)
                     /gene="amrB"
                     /locus_tag="PKV64_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPN17256.1"
                     /translation="MKRAAAVAGSFYPASASELRRMLGSLVDPARKKVKAAAVVCPHA
                     GYVYSGAVAGAVYSSVEMPGTFVILAPAHRPQRPAFAIMSEGSWETPLGSVPVDEELA
                     AAIRAKCPAVVDDPAAHAAEHSLEVQIPFLQHLRKSPAIVPLNVSARADFPRLREAGE
                     AIAAAVRESGKDVLLVASTDMSHYISADRAKLLDGLAIDRILALDARGLVETVFDNDI
                     SMCGVLPTAAVVVAAAALGATRAELVAYANSGEVTGDDREVVAYAGLRIY"
     gene            complement(7953..9275)
                     /locus_tag="PKV64_09030"
     CDS             complement(7953..9275)
                     /locus_tag="PKV64_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPN17257.1"
                     /translation="MKHLLVIGAGFVAGPLVRTFLEKPDVRVTVADIEPEKARALTGT
                     HERGRAVVLDLNDEAALRNEIKGADLVVSLVPYVFHPVVARLCLAEKKNLVTTSYVSE
                     AMRSLDADARRAGIIFLNEVGLDPGIDHMEAMKGIDGIRSRGGRVLGFRSYCGGLPAP
                     EADTNPFGYKFSWSPRGVLLAGKNEARYLKDGREVVIPAADLFLNAETIEIPGLPAFE
                     GYPNRNSLSYLETYGIPEARDIFRGTLRWPGWCETLKKIGDWGLLDAKVSDWTGLTRR
                     PFLARTAGLPDGADLRRELAARWKIDPEAKLLDRLAWLGLFEDGPLPGKSGSPLDLLE
                     KLMLAKMLYAPGERDMVVLQHEFRAAFPDGTAERTVSTLIDYGIPGGDTSMSRTVGLP
                     AAAAADLVLKGRLNRTGVLTPVTPDVYGPILAELKSRGLVFREETISE"
     gene            complement(9307..10617)
                     /locus_tag="PKV64_09035"
     CDS             complement(9307..10617)
                     /locus_tag="PKV64_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17258.1"
                     /translation="MKNVIALRREDRNPFEKRVALIPSHVRELVKDHGVKFLVQPAPI
                     RVFVDDDFKREGAKVEEDLSSAAVILGIKEMPDSIFEKDKVYVFFSHTIKGQKANMPM
                     LRRMIERKSTLIDYEKIVDEKGRRLVFFGRQAGTAGMIESFWALARRLDAEGLPHPFG
                     PIRRMIDYASLVEAKETINEIGEGIRKDGLPAHLVPFICGFTGYGHVSQGAQEVFDIL
                     PEVEIPAEEFDAFLAAGNFSAHRLYKIVFREEHLVRPRDPGRPFDLQDYYDRPEGYVP
                     VLESFLPHLQLLVNGIYWAPKYPHFVSKKFIRELYAAPGRPKLRVIADISCDIEGGIE
                     CTVKDTDPHNPVYVYDPVKDAAVDGVEGRGPVIMSVYNLPAEIPLESSVFFSQTLKPF
                     IPAIARADFRGTFEACELPDPIKKAVILFRGEFTPAYQYMKEFV"
     gene            10735..>11040
                     /locus_tag="PKV64_09040"
     CDS             10735..>11040
                     /locus_tag="PKV64_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPN17259.1"
                     /translation="MIRFHNTLSGREEEFLPLVPGEVRVYTCGPTVYNVPHIGNFRAY
                     VFEDLLLRFLRFSGYKTIQVMNLTDVDDKTIRDARTAGLSLREYTAPFIESFFAGLKT
                     "
BASE COUNT         1943 a         3417 c         3467 g         2213 t
ORIGIN      
        1 ggccagctgg ttccggatct gccgctgctg gtggggcggg aaaacgccga cgatgcggtt
       61 gaccgtctcg ggggcgtcga gggtatggag ggtgctgagg acgagatggc ccgtctcggc
      121 cgcggtcaag gctgtttcga tcgtctcctg gtcccgcatc tcgccgacca gaatgatgtc
      181 cggatcctcg cggagggccg cccggaggcc gcgggcgaag gatttgacgt cccaaccgac
      241 ttcccgctgg ctgatggagc ttttccggtc ccgatgaagg tattcgatcg ggtcctcgat
      301 cgtgatgatg ttttcgcgcc ggaggccgtt gatgtgatcg atcatcgaag ccagggtcgt
      361 cgtcttgccg cttcccgtgg tcccggtcac caggaccagc ccccgggtgt agtttgcgat
      421 tttttccaag atctccggaa ggagaagctc cttcagggtc ttgatctcga aatggatgat
      481 ccgcagggcg atggcgaccg aggatctctg gtaatagatg gacccccgga atcgaccgaa
      541 gccggggagg ctgtaggcca ggtcaatgtc caattcctcc ttcaggcggt cccgctggga
      601 ctcgcccatc atttcagcgg ccaggttttc cgtgtcgtcg atggacaggc gcgggatttt
      661 ctccaggacg accaactggc cggcgacgcg gacctgcgga tgggtgcccg ccttcagatg
      721 aaggtccgag gcttccattt caatggcgat tttcagcaaa tcatcgatat gcacggctcc
      781 tccctgggtt ccacccgggc tgattcccga ttatcttacc gggattgggg aatcgggtca
      841 atcgacgccg aggccgacgg ccgctttctc ctttttcagc atgcttcgcc gcagtagggg
      901 cacgttttag cccggccggg aaggtgccgc cggcagttcc agcaagttgt cgtcgtcgcc
      961 ggttcccggg cccggatccg cccgaggatg tcgtcgattt cgttcttggc cggcttctcc
     1021 cccttgatct cgttcaaagc ccggatgatt tcgaagggac gctggaaacg gtcctcgatc
     1081 cggttggcca ggcagcgcat gatgagcgcg tcgatttccc ggggaacccg ggtgtttttc
     1141 aagcggggcg gcgtgatccg gccttcctgg gcgcgctcca ggatcttaaa cgggtcgggg
     1201 tccaggatgg gcggccgccc gatcatcatc tcgtacagaa tgcagccgag ggaatagata
     1261 tccgaggcgt acgtggcctt gcccatgaac tgctcggggg ccatgtacgg cggcgatccg
     1321 atccgggtcg tcgcataggg gacgtcctgg agccaggccg aggttccgaa atccgtgatt
     1381 ttcacctggc cttcctcgga gacgatgatg ttggagggcc ggaggtcccg gtggatgatc
     1441 ttgtttttat gggcatggtc gacgccggcg gccagttgtt tcatcatgtc gatggccgcc
     1501 gcggtctcca ggactttttc tttctggaga atcttttcca gggtccgccc gcggacgtat
     1561 tccatcacga aaaagaatat cttgtcgatc ttttcagccg ccagcagccg gacgatattc
     1621 ggatgatcca ggacggcctg gagacgcggc tccttcagga gcttataaag ctcgatggat
     1681 tgcttatggg gaaccttgat cgcgaccttg atgtcgagcc agatgtcctt ggccaggtaa
     1741 accgaaccga atcccccgct gcccagcgag cggatgattt catatttgcc gattttctgt
     1801 ccctggagaa acatccttca ccagttccat tcgtgggcca taaccgccag ggccgccaag
     1861 gcggccgtct ccgcccggag aatcgtctct cccaggctca ccgcttcgaa ttccgccgcc
     1921 cggatggcct cttcctcgcg ggcgctccat cccccttcag gcccgacaag aaccgtcgcc
     1981 tccgccggcg gcgcctcttc cccgccccgc agcgacttga gactcatccc gccgtgctcg
     2041 cacaggaaaa ttcgccgccc gtcccggccg gaggccagga aatcggccag ggaggccggg
     2101 gcatcgatga gcggaacccg ggcggcccgc gactgcttgg cggcggcccg ggcgagtcgc
     2161 gtccagcgtt ccaccttccg ccgggaatgg tccccggacc gggctaccga ccgctcgctt
     2221 tcgacgggcg atatccggga cgcgcccagc tcgcacgctt tcaaaatgac ttcatccatc
     2281 gccccggtct tgagaagggc gggcgcgaga atgatccgcg tccggacgcc gcggggagga
     2341 aggacctcca gaatcgaaag gtcgatttcc tcctccttct cccggacggc ggccgcttcg
     2401 gctcgatacc ggcggccgtc ctcgtcgaaa agccagacgc ggccgccggg acggacccga
     2461 agcacccgtg cgagatggtg gtattccgcc ccccgcagaa cggccgaaga cccctcgaca
     2521 tcggctcggg ggagaaaaaa atattcggaa gtcaaggatg ccctaccggg atcccgggga
     2581 agcccggcgg acggcgccgg cctccaatcg atccagattt tcccgccgcg attcggccag
     2641 ccggcggaga agctcttttt cctcccgcga cagctcggcc ggcgtttgaa cgtccacctt
     2701 gacgaacagg tcgcccgtcc ggtggcgcga aagatccttc aagccgcatc cttttatgcg
     2761 gaaaatctcg cccgactggg ttccagccgg gattttgagc ttttccgtct tgcccgaaag
     2821 agaggggatt tccgccgtga cgcccagcgc ggcctggctg aacgacagcg aaagcccgca
     2881 gatcaggttg ttctcctccc gctcgaaaat atcatgaggc ttgatccgga tatccacgta
     2941 tagatccccc cggcccgacc ccgggtcccc cgcttcccct tcgccggtca tccgaaggcg
     3001 gatgccgtcg gccacccccg cgggaattcg gattttcaag gtccgtttct gcgttttacg
     3061 gccgccgccg cggcaatccg gacagggatc ggtgatcact tcgcccgtcc cgcggcatct
     3121 gccgcagggg ctggcgatgc tgaaaaatcc ctggctgcgg cggacttggc ctcggccgcc
     3181 gcaagccgga caaacggcgg ggcgcgtccc cgcccgggct ttcgacccgc cgcaggaggg
     3241 gcacagctcg gcccgctgga tcgtgatgtc cttatcgacg ccggcggccg cctcctcgag
     3301 ggtgatttcg accccgagac ccaggtcccg gccgcgggcg gcccggggcc gcctcgccgt
     3361 tccgccgaag ccgaaattga acccgaagaa atttcccagg atgtcctcga attcctcgaa
     3421 gatcgaaccc gagaatccct cgaaggccgg gccgcgcagg ccgtccggac cgaagcggtc
     3481 gtacgtctcc cgtttttgag gatcgcccag aacgctgtac gcttcagccg ccttcttgaa
     3541 ccgctcctcg cagtccttgt cgccgggatt gcggtccggg tgatatttca tcgccagctg
     3601 acggtagcat ttcttgattt cctcggagct ggccgtccgg gaaacgccga ggacctcgta
     3661 atagtcttcg tttgccatgg acgttcgtct cagtgcgcct ttttcttttc ccgctcgata
     3721 atgttttcga cttgctgcgg gctcagaccc gaagaggggt ggacggaaac tttttgctcc
     3781 cggccggaaa ggacatcccg ggccgagacc ctgaccatgc cgtcggcgtc gatctcgaac
     3841 gtcacgtcga tgcggggaac cccggccgcc gcatccatga tcccgacaag gtcgaacatc
     3901 gccagggagg tgttttccaa ggcccggctt ccctcccctt ggagcacgtg gatccggacc
     3961 cgccgctggt tgtcgtcgac cgtggtgaag gatttcgttt tccggcaggg aatggtcgtg
     4021 tttttatcga tgatcttctc gaattccccg ttctccgtct cgatccccag cgacaaggac
     4081 gtcacatcga ggaggaggac gatgtccttc gccccgttgc cctcgagaac gccggactga
     4141 atggccgcgc cgagagccac gctttcgtcg gggttgatct gggtgttcgg atccttgccg
     4201 aagaactcgc cgactttttt ccggaccagg ggcatccggc tctgcccccc gaccaggagg
     4261 atctcggcga ccttgttcgc cgtcaaaccg ctttcgtgga gcgccttgtc catcagcggc
     4321 agggtccgct ccaccaggtc ccgggtccat tcctccaggt tcgcccgggt gatcgcggtt
     4381 tggagatgct tggagcccga tgcgtcggcg gcgatgaacg gcaggttgac ttccgtgatc
     4441 gcggtgaagg acaggtcccg cttggcccgc tcggccgctt ccttcaggcg ttggagattg
     4501 tagggattag atccgaggtc gatcccgtgc ttgcttagaa aatcggcccg gaggcgatcg
     4561 atgatcctct gatcgaaatc atcgccgccg aggaagttgt ccccggcggt cgccaagaca
     4621 tggaaaacgc ccccgctgat ctcgaggagg gtgatatcga acgtccctcc gccgaaatcg
     4681 tagacggcga ccacgccgtc gggattccgg ttgagcccgt acgccaggct ggccgcggtc
     4741 ggctcgttga tcacccggag gacgtccaga ccggcgatgc gggcggcgtc cttcgtcgct
     4801 tgacgttgat ggtcgtcgaa atgggccggg accgtgatga cgcattccgt gacgggctcg
     4861 ccgaaaaatg actcggcgca gcgcttcaga tagaccagga tgtggctcga cgcttcttga
     4921 ggggtgatcg ttcggtcgcc cgcccggatc aagacgtcgc cgttgggagc ctcgctcaag
     4981 ccgtacgtca gccgcggccg cgcctcctgg acggccgggg aactgtattt gttcccgatg
     5041 agccgcttga tggcgaagat cgtcttttcg ggattggcca gggcctggcg ggcggccagg
     5101 ttcccgacaa ggatctcgcc ggcggacgtg aaacagacca ttgaaggcgt ggtcgccatg
     5161 ccctccaggt tggggatgac cttgggacct tcgggcgaca ggtgagccac gcaggaaaac
     5221 gtcgttccta agtcgattcc gatgacacgg ctcatgtcag tttttcttag gcatcaaaac
     5281 tttaaccagg gcggggcgca agagccggtc attcagcatg tatcccttct ggagctcctc
     5341 cccgatcatc ggctcctcga tcccctcggc ctcctcggtc aggagggctt ggtggacatt
     5401 cggatcgaac cggccgtcgg ggcgctcgat gagggtcacg ccgcgcttgc cgaccaggtc
     5461 gagcatttgc cggcagatca gctcgacgcc ttctcggagg ccgtcggaag cgtcccgggc
     5521 gtccagggcc ctctcgagat tgtcgatgac ttggagaaac tcctttaaaa tatcggaaag
     5581 ggagaagagg aagaattcgt ccttttcccg ctcgagccgc ttgcggaagt tatccgtgtc
     5641 ggccatggcc cgcagagtcc ggtccttcca ttccaaggat tccttccggg ccgtttcgac
     5701 ctcctcgcgg gccgcggccg cctcccgggt ccgttgttcg aggtccttct tcagggcttc
     5761 gatttccgct ttcaaagact tgattttttg atgatccttg ttgttcttgt ggttccggtg
     5821 ctcggacgtc tttttccggc ccgccgagtc gggctcgacg ggaggagcgg ggatcgcgcc
     5881 ctcgggcgcg gccgcttccg ccgagggagc ggaaaggacg tccttttcgt cttggatttt
     5941 tcgatcttcg ttaatcatag gcatttttcc taggagctcg ccccggatat ggtccggctg
     6001 attttttgcg cgacgttatc gaccaggggg atgattttat cataggggat ccgcttgggg
     6061 ccgatgatgc cgagggatcc caaaacttgg gtccggtccc cgtaatggga gaggaccagg
     6121 gcgcagtcgg agatctcggg catgttggat tccgatccga tcaggacctt gacccggtcc
     6181 agggcgatga attccgaaag gagcttggcc agggaggcct tctcctcgat attttgaaac
     6241 agcattttca accgcgggag atcggctttc tcgaatttgc cgatgagccg ggacgctccc
     6301 tggacgatga tcggatcctc catgtcatcc tgctggatcg aggctttcag gaggctcgtc
     6361 aggcgggaca aggcccgctc gaacttggac gtcgatttgc ccagctcctc aaggacgcaa
     6421 tcccgcactt gcgacagcgt ccggccgctg aacgaggcgt tgatatactg ggaggcccgg
     6481 tcgagctcca cctggtcgaa agtcgtatcg gtcggaacga tttcggtcaa gaccagctga
     6541 aagggcgtca tcaggatgat catcaccttg cccgcggcga tctggatgaa gcgaatatgg
     6601 cggaagtgga tgcgcgagat ccggggttgg atgacgaatc ccaggctgtc ggattgatcg
     6661 gccaacaccc gggaggcctg gtggagaagg ccggggagat cgctcccgcc gagggaaagg
     6721 tcttcggcga acaggttgat ccggtcgaag gtcaggggga cgcccgccag caggctgttg
     6781 acgtagaagc ggagcccgcg gtcggtcgga atccgcccgg acgaggcgtg gggctgcgaa
     6841 aggtagcccc tctcccccag gcggacgagg atattgcgga tggtcgccgg ggaatccgtc
     6901 aggactttct tttggtgaat ggcgccggac gagacgggca ggccggtttt caggtacaac
     6961 tcgacggccg cggccaggac cagccggtct ttctcccgta actcctgatt tttcatcttc
     7021 gtccttgaaa attttataag aggacggcga gcctgtcaac gaatcagcgg cagccctcct
     7081 ggacaaacgc gcccgccgct ccggggccca ggcgcctcga ctcttaataa atcctcagtc
     7141 cggcgtaagc cacgacctcc cggtcgtcgc ccgtgacctc ccccgagttc gcgtaggcca
     7201 ccagctcggc ccgggtcgcc ccgagggcgg cggccgcgac gacgaccgcg gccgtgggca
     7261 ggacgccgca catgctgatg tcgttgtcga acaccgtttc aacgagaccc cgcgcgtcga
     7321 gggcgagaat ccggtcgatg gccaaaccgt ccaacagctt cgcccggtcg gcgctgatgt
     7381 aatggctcat atccgtgctg gccaccagga ggacgtcctt cccgctttcg cgaacggcgg
     7441 cggcgatcgc ttctccggcc tcccgcagcc gggggaaatc cgcccgggcg gagacgttga
     7501 gggggacgat cgccggcgat ttccgcagat gctggaggaa cgggatctgc acttccagcg
     7561 aatgctcggc ggcatgggcg gccggatcgt cgacgactgc cgggcacttc gcccggatcg
     7621 cggccgccag ctcttcatcc accgggacgg atcccagcgg ggtttcccaa gagccctcgc
     7681 tcatgatcgc gaaggccggc ctctgcggcc ggtgggccgg agccaggata acgaacgttc
     7741 cgggcatctc gacggaggaa tagacggctc cggcgacggc gccggaatac acgtacccgg
     7801 cgtggggaca gacgacggcc gcggccttta cttttttccg ggctggatcg acgagcgacc
     7861 cgagcattct ccgcagctcc gaggcgcttg ccgggtaaaa cgaaccggcg accgcggccg
     7921 cgcgtttcat ggcgccctcc cctattctga attcattcgc tgatcgtttc ctcgcggaaa
     7981 acgaggccgc gggacttcag ctcggccaaa atggggccgt agacgtcggg cgtcaccgga
     8041 gtcagcacgc cggttcggtt cagacggccc ttcaggacga gatcggccgc ggccgccgcc
     8101 ggcaggccga ccgtccggga catggaggtg tctcctccgg ggatcccgta atcgatgagc
     8161 gtcgaaacgg tccgctcggc tgttccatcc gggaaggcgg ctcgaaattc gtgctggagc
     8221 acgaccatgt cccgttcgcc cggggcatag agcatcttgg ccagcatcag cttttccaga
     8281 agatccaggg gcgatccgga ttttccggga agcggaccgt cctcgaacag tcccagccag
     8341 gccaaccggt ccaggagctt cgcctccgga tcgattttcc agcgggcggc cagctcccgg
     8401 cgcaggtcgg cgccgtccgg caggccggct gtccgagcca ggaacggccg ccgggtcagg
     8461 ccggtccaat ccgatacctt ggcgtccaga aggccccagt cgcctatctt cttcagcgtc
     8521 tcgcaccatc ccggccaccg gagcgtcccc cggaatatat cccgggcctc cgggatgccg
     8581 taggtctcca agtacgaaag ggaattccgg ttgggatatc cctcaaacgc gggcaagccg
     8641 gggatttcga tcgtttcggc gttcaagaaa agatcggccg cgggaatgac gacctcccgg
     8701 ccgtccttga gatagcgggc ctcgtttttt ccggccaaaa gaacgcccct cggactccag
     8761 gaaaacttgt agccgaaggg attcgtgtcg gcctcgggag ccggcaggcc cccgcaataa
     8821 gaccggaagc ccagaacgcg cccgccccgg ctccggatgc cgtcgatgcc cttcatggct
     8881 tccatgtggt cgattcccgg atcgaggccg acttcattga gaaaaatgat ccccgcccgg
     8941 cgggcgtccg cgtccagaga ccgcatggcc tcgctgacat aagaggtggt gaccaagttc
     9001 tttttttcag ccaggcagag ccgggcgacg accgggtgaa agacgtaggg cacaaggctg
     9061 acgacgagat cggccccctt gatttcattc ctcaaggcgg cttcgtcgtt cagatcgagg
     9121 acgacggccc ggccgcgctc gtgcgttccg gtcagggccc gggctttttc cggctcgatg
     9181 tcagccaccg taacccggac atccggtttt tccaggaacg tgcggaccag cgggccggcg
     9241 acgaagccgg ccccgatgac gagcaagtgt ttcatcggtt tctctctttc cgtgaggatg
     9301 attccctcac acgaactcct tcatgtattg ataagccggg gtgaattccc cgcggaacag
     9361 gatgaccgct ttcttgatcg gatcgggaag ctcgcaagcc tcgaacgttc cccggaaatc
     9421 cgcccgggcg atcgccggaa tgaacggctt gagggtttgg ctgaagaaaa ccgacgattc
     9481 caagggaatc tcggccggaa gattgtagac cgacatgatg accggtcccc ggccctcgac
     9541 gccgtcgacg gccgcgtcct tgaccgggtc atagacgtac acgggattgt ggggatcggt
     9601 gtccttgacc gtgcactcga ttccgccctc gatatcgcag gagatgtcgg cgatgacccg
     9661 cagcttcggc cggccggggg cggcatacag ctcgcgaatg aatttcttgg agacgaaatg
     9721 cgggtacttg ggggcccaat agatgccgtt gacgagaagc tgtaaatggg gcaggaagga
     9781 ttccaggacc gggacatatc cctccgggcg gtcgtaataa tcttggagat cgaacggccg
     9841 gccgggatcc cggggacgga cgagatgctc ctcccggaag acgatcttgt agaggcggtg
     9901 ggccgagaaa tttccggcgg cgaggaaagc atcgaactct tcggccggga tttccacttc
     9961 cggcaggatg tcgaagactt cttgggcgcc ttgggagacg tggccgtagc ccgtgaagcc
    10021 gcagatgaac ggaacgagat gggccgggag gccgtccttt cgaatcccct ccccgatctc
    10081 gttgatcgtt tccttcgcct cgaccaggct cgcgtaatcg atcatgcgcc ggatcgggcc
    10141 gaagggatgg ggcaggcctt cggcgtcgag tcggcgggcc agggcccaaa aggattcgat
    10201 catcccggcg gtgccggcct ggcggccgaa aaagaccagc cgccggccct tctcgtcgac
    10261 gatcttttca taatcgatca gcgtgctttt cctctcgatc atgcgccgga gcatcggcat
    10321 gttggccttt tgccccttga ttgtatggga gaagaagacg tagaccttgt ccttctcgaa
    10381 aatcgagtcg ggcatttcct tgatgccgag aatgaccgcg gcggaagaga gatcctcctc
    10441 caccttggcc ccctcgcgct tgaaatcgtc gtccacgaac acccggatcg gagcgggctg
    10501 gacgaggaac ttcacgccgt gatctttgac aagttcgcgg acatggctgg ggatgagggc
    10561 gactcgtttc tcgaacgggt ttctgtcctc tcggcggaga gcgatgacgt ttttcattat
    10621 acacccttaa atgattgatt tggtatgagt taagatacat tatggcataa tttcacgacg
    10681 cggtcaaatc cgattcttct ttttcgacga gggagccggt ataatgaggg gcccatgatt
    10741 cgttttcaca acaccctcag cggccgggag gaggaattcc tgccgctcgt tccgggcgaa
    10801 gtccgcgtct acacctgcgg accaaccgtc tacaacgttc cccacatcgg gaatttccgg
    10861 gcgtacgtct ttgaggacct gctcctccgg tttctccgtt tttccgggta caaaaccatc
    10921 caggtcatga acttgaccga cgtggacgac aagacgatcc gggatgcccg aacggccggt
    10981 ctttccctcc gggagtacac cgcccccttc atcgagtcgt ttttcgccgg ccttaagacg
//
LOCUS       DAOOXH010000081        10997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_6374_length_10997_cov_7.054286, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000081 DAOOXH010000000
VERSION     DAOOXH010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 10997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10997
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6374_length_10997_cov_7.054286"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PKV64_09045"
     CDS             complement(<1..571)
                     /locus_tag="PKV64_09045"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HPN17260.1"
                     /translation="MEFLLEINVEEMPLAHVRTALADLSERLRRDLAAARIPCRDLRT
                     FSTTRRLILTAFLAPGQDERDELVTGPPKSAAFAADGTPTKAALGFARSKGVDVSRLQ
                     IVTTEKGEYVGVRRTVKGRPAADLLAEAIPSIITSLSFPKMMRWGTGTLRFSRPIRGL
                     LCLLGGAVVPFSLDGLVSGSRTTGHKLHVH"
     gene            complement(571..1431)
                     /locus_tag="PKV64_09050"
     CDS             complement(571..1431)
                     /locus_tag="PKV64_09050"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HPN17261.1"
                     /translation="MSLQRLILDLHRFWADQGCYLAPTYDLEVGAGTMTPDTFFRVLG
                     PRPWRTAYVQPARRPTDGRYGENPNRVQKHHQYQVILKPSPADIQNVYLDSLRACGID
                     LRDHDLRFDEDDWESPTIGAWGVGWQVMLDGLEVSQFTYFQQAGGLEVSPIPVEITYG
                     LERLEMFIEGKDDIYDLQWSDDVTYRDLRLDEERQFSDYNFNRATVKTLRLQFAALEK
                     EAAMLIDQGLPLPAYDQCLKCSHAFNLLDARGAISVTERVKMIAQIRTLVSRVARLFT
                     APAPSGEGTA"
     gene            complement(1428..2885)
                     /gene="gcvPB"
                     /locus_tag="PKV64_09055"
     CDS             complement(1428..2885)
                     /gene="gcvPB"
                     /locus_tag="PKV64_09055"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPN17262.1"
                     /translation="MIREPLIFEISDPGKKGYAVPKLDVPAAAEALAGLPVREGIPGF
                     PQVSEVEVVRHFTRLSQQNYCVDLGLYPLGSCTMKYNPKVNEKIAGLPGFAEVHPLAP
                     VETIQGNLEILYRTERLLCEITGFDAVTLQPSAGAQGELTGIMLARACHESRGNPRKI
                     VLIPDSAHGTNPSSAHLSGYEVREIKSNAAGRIDLGSLQQAMTDEVAALMVTNPNTLG
                     VFESDICKIADIVHGKGGLLYMDGANMNALAGIARPGDFGVDVLHLNLHKTFATPHGG
                     GGPGSGPVAVKKILEPFLPVPVVAQKGSAYALDFDRPRSIGRVRSFYGNFLVVLKAMA
                     YILSLGPRGMREVAEIAILNANYIRKGLEGVFDLPYDSASMHEAVFSDKIQKDAYGVT
                     NLDIAKRLIDHGFHPPTMSFPLIVPGALMIEPTETESRRDLDLFIEAMKTIAREAAEN
                     PALLKAAPHQTYVRRLDETAAARNPVVTWEGGQDR"
     gene            complement(2882..4228)
                     /gene="gcvPA"
                     /locus_tag="PKV64_09060"
     CDS             complement(2882..4228)
                     /gene="gcvPA"
                     /locus_tag="PKV64_09060"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HPN17263.1"
                     /translation="MSYIALSDQDKKEMLARLGVSSVEELFGCIPEDSRLRRDLNLPP
                     AMSEPELLGHMDAVAGRNTYGRHRSFLGGGTYHHYIPAVVDSLSARTEFLTPYTPYQP
                     EVSQGTLQVIFEYQTLICRLTGMDIANASLYDGASAAAEAALMARRLTGRSRILVARS
                     LHPQYRETVRTLTRNLGLAVEEVGFGGDGGLNPEELRAKTGPDVCGLIVQSPNFFGVV
                     EDLAACAAAVHESKGLLIVSVAEALSLGILEAPGKLGADIVCGEAQSFGIPPSFGGPG
                     LGFMGCKKEYLRQLPGRICGLTKDVEGRRGFVLTLSTREQHIRREKATSNICSNQGWC
                     ALRATIYLESAGQNGLRDIALQNVRKAAYAAGKLAAVGGIRLRFTGKTFNEFVLQLPK
                     DAAAAAAALRAKGILAGLELAPLYPELPGTMLVCVTEVRSKEEIDGLAAALEEVLR"
     gene            complement(4250..4636)
                     /gene="gcvH"
                     /locus_tag="PKV64_09065"
     CDS             complement(4250..4636)
                     /gene="gcvH"
                     /locus_tag="PKV64_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPN17264.1"
                     /translation="MYPQDYKFSKEHEWVKVDGDTAVIGISDYAQNHLGDVVYVDLPA
                     VGTKLTAHQTIGSIESVKAVSEIFSPVSGEVIETNAGLTDAPEKVNQDPHGQGWILKL
                     KMADKGDLNALMNAADYEKYLEGLEH"
     gene            complement(4679..5767)
                     /gene="gcvT"
                     /locus_tag="PKV64_09070"
     CDS             complement(4679..5767)
                     /gene="gcvT"
                     /locus_tag="PKV64_09070"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPN17265.1"
                     /translation="MKQTRFNAHHRRLGAKMVDFFGWDMPVEYKGIIDEHVACRTAAG
                     LFDVSHMGEVHVTGKDALAFIQHVSSNDAARLSPGMIQYSGLMTDKGTYVDDMLIYCL
                     HPEFFLLVVNAANTDKDFAYLTAHEGAYDVVLDNASDRYSQLALQGRQAIPILQPMTE
                     IPLAGMKPFTFAFGKVAGKECLVSRTGYTGEDGFEIYTLDPDPGILWDSLMEKGTPLG
                     MQPVGLGARDTLRLEARLPLYGNDIDDTTTPLEADFKWIVKFKKGDFIGRDVLARQNE
                     EGITRKLVGFELIDRGIARAHYPVFVEGAPAGQVCSGTFAPLLKKSIGTVYLPAAKAA
                     VGTEFEILIRDKKARAKVVPSPFYKRDY"
     gene            5889..6290
                     /locus_tag="PKV64_09075"
     CDS             5889..6290
                     /locus_tag="PKV64_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17266.1"
                     /translation="MKNPVEIAKDGAIYAILVRAESAGRGPRFFTPDDFSQQLGLLVH
                     PKGRTVERHRHKAGKGPVVRTQEVLVVLDGKMRLDLFDESGTKLKAVILKAGDSILLA
                     RGGHKATFLTDSRILAVKQGPFAGPGEKEFY"
     gene            6327..8354
                     /locus_tag="PKV64_09080"
     CDS             6327..8354
                     /locus_tag="PKV64_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPN17267.1"
                     /translation="MGETSVLTTLVFILAAALFVVAVSQKLKILAVVGFMLTGVFLGP
                     SGVGLVKSDATISLLADVGVMMLLFVIGLDFSLRAVVAARRPFFLGGGLQVGLTTGAV
                     AAIFIALGFRPGEAVIIGFIAANSATALVAKILADRGELDSPHGRTIMAITLFNDLIA
                     VPVIALIPLLAGRAAGSTGVIAKFGWSLAAITAAFFFLRAAVPRLLEVIIRTRIRELF
                     ILSSLLLCLGLALFSGALGLSMAFGAFLAGLIVADSPYSHQMAADILPLKDVFISLFF
                     ISIGLLLDIRIFSRAMGLAAAMAVGVIILKTLINSVVLKILKLSPRGILLGSLGVAQI
                     GEFAFVLAGAARAHGFLAGPGFQLFLSSAILTMIVSPYLLRAADALSAGAARKALRKA
                     DGAAEEKESPAAGMNGHVIIVGYGLNGQNLARVLRTAGIEHLVIDLNPKLIRQAVEDG
                     EKTLFGDVCRTEILRLAGIRRAKIIVFAISDATATRRGLLLVRGLGSKAQIIVRSRYV
                     TGLDALYEAGADSVIPEEFETSIEIFSQVLEAYHIPKNIIDAEVKAVRAERYGILRAT
                     GPVRPSFEKIVDLLTAGTAETFYVGPDCPAAGRTLAQLDLRARTGATVIAVVRGEESH
                     TGPAADFLVEPGDTLVLVADHAAMDVAFDYLAARAAREAGVPPAGSFPPRS"
     gene            complement(8367..9281)
                     /locus_tag="PKV64_09085"
     CDS             complement(8367..9281)
                     /locus_tag="PKV64_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPN17268.1"
                     /translation="MIRQRLGWIPDIPDFRDYSGETPVVRDLMAGARRRPGLPPQRAG
                     GGGRPAGASEPLPPAVDLRSHCSPVEDQGNLGSCTANAGTGIIEYYERRAFGRHLEAS
                     RLFLYKAARNLMKISGDTGAYLRTTIGAMVLFGVPPEDYWPYDDRPDRFDREPPAFCY
                     AFAQNYKTIRYYRHDVPGTPAGETLDRVKTHLAAGHPSMFGFTVYGSIEAAGGDGAIP
                     FPGPRERIEGGHAVAAVGYDDRLKIRNEKNGGGETTGALLIRNSWGKGWGDGGYGWLP
                     YAYVLRGLAEDFWSVLKKDWIDTGAFDE"
     gene            9469..10188
                     /locus_tag="PKV64_09090"
     CDS             9469..10188
                     /locus_tag="PKV64_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17269.1"
                     /translation="MKSWRVKAVLAAAALTILSGAVPSSAQFEFQADVMSKYIWRGWD
                     LYWNNQPALQPSLTYAFGESGFSVNLWGSFALGERPVYKDYDEIDLTLDYTFSLSDHL
                     EMSAGFINYGYFFGGKDVCKENTTQEFYVSAGWPEILLSPTLTLFYDFNLGSGLYALF
                     EASHSVAISESLGLDLCAELGYNSRQFIEENGFSDLTIGASLPFTLGDWTIAPLIRYT
                     FVFLDAVNEDDELWFGISLIR"
     gene            complement(10195..>10997)
                     /locus_tag="PKV64_09095"
     CDS             complement(10195..>10997)
                     /locus_tag="PKV64_09095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPN17270.1"
                     /translation="LNTMGNAAFQLIDRDPAGLKAAVLQAAEQIGERRLLTAAAEAAG
                     ITFSQEEFDKVLAEQYAKAGGEEAFLEMLKSNKVDPESFKRMMREEQMIGKFLEADVF
                     QKITVGEEELRQAYAADKTATVRHILLMTQGKPESEIPEIRKKMEDLLARARKGEDFA
                     ALAREYTEDPGSKENGGLYEDFPRGMMVKPFEDAAFSVPVGELSGVIETKYGFHILKV
                     ENRMKETEPFEAVRTRLEDNLKLAKRAGVYENFVAGLKSKAGFVEVKS"
BASE COUNT         1857 a         3459 c         3568 g         2113 t
ORIGIN      
        1 cgtgaacatg aagcttgtgg ccggtcgtcc ggcttcccga gacgaggccg tccagggaaa
       61 acggcacgac ggcgccgccc aggagacaga gcaggccgcg gatcggccgg gaaaagcgga
      121 gcgtccccgt tccccagcgc atcatcttgg gaaaggagag cgaggtgatg atcgacggga
      181 tcgcctcggc cagaagatcc gccgccggcc gtcccttgac cgtccggcga acgccgacat
      241 attccccctt ttcggtggtg acgatttgga gccgggacac gtccactccc ttgctccggg
      301 cgaagcccag ggccgccttt gtgggcgtcc cgtcggcggc gaaggcggcc gacttggggg
      361 gaccggtgac caactcgtcc cgttcatctt ggccgggggc tagaaaggcg gtcaggatca
      421 ggcgccgggt ggtcgagaag gtccggagat cccggcaagg gatccgggcc gcggccagat
      481 cccggcgcag ccgctcggac aggtcggcca gggccgtccg gacatgagcc aggggcatct
      541 cctcgacgtt gatttcaaga agaaattcca tcaggccgtc ccttccccgg acggcgccgg
      601 ggcggtgaag aggcgggcga cccgggacac cagcgtccgg atctgggcga tcatcttgac
      661 ccgctcggtt acgctgatcg ccccccgggc gtcgaggagg ttgaatgcgt gggagcactt
      721 gaggcactgg tcgtaagccg gaagaggcaa gccttggtcg atcagcatcg ccgcttcctt
      781 ttccaaagcg gcgaactgca accggagcgt cttgaccgtg gcccggttga agttgtagtc
      841 cgaaaattgt cgctcctcgt ccaggcgcag gtcccggtac gtgacgtcgt cggaccattg
      901 gaggtcgtag atgtcatcct tgccctcgat gaacatctcc agccgctcca gcccgtaggt
      961 gatttccacg gggatcgggg aaacctccaa gccgccggct tgctgaaagt acgtgaactg
     1021 ggagacttcc agaccgtcaa gcatgacctg ccagcccacc ccccaggcgc cgatcgtcgg
     1081 cgattcccag tcgtcctcgt cgaagcggag gtcgtggtcg cgaaggtcga tgccgcaggc
     1141 ccggagactg tcgaggtaaa cgttctggat gtcggccggc gagggcttga ggatcacttg
     1201 atactggtgg tgtttctgaa cgcggttggg attttccccg taccggccgt cggtcggccg
     1261 ccgggccggc tggacgtagg ccgtccgcca gggacgcggg ccgagaaccc ggaaaaacgt
     1321 gtccggggtc atcgtccccg ctcccacttc gaggtcgtac gtcggggcga gatagcaccc
     1381 ctggtccgcc cagaagcgat gcaggtcgag gatcaagcgc tgaaggctca tcgatcctgt
     1441 ccgccctccc aggtgacgac gggattgcgg gccgcggccg tctcgtccag ccgccggaca
     1501 tacgtctggt gcggggccgc cttgaggagg gccggattct cggccgcttc ccgggcgatg
     1561 gtcttcatcg cctcgatgaa caggtcgagg tcccgccggc tttccgtctc ggtcggctcg
     1621 atcatcaggg cgccggggac gatcaagggg aaagacatgg tcggcggatg gaagccgtgg
     1681 tcgatcagcc gcttggcgat gtcaaggttg gtgacgccgt aggcgtcctt ctggatcttg
     1741 tccgagaaga cggcctcgtg catcgaggcg gaatcgtacg gcaggtcgaa gacgccttcc
     1801 aagcctttgc ggatgtagtt ggcgttgagg atggcgatct cggcgacctc ccgcatcccc
     1861 cgggggccga gggaaagaat ataagccatg gccttcaaca cgaccaggaa attcccgtag
     1921 aaggaccgga cccggccgat cgaccgcgga cggtcgaaat cgagggcgta cgcggatcct
     1981 ttttgggcga cgacggggac gggcaggaag ggctcgagaa tcttcttgac cgcgaccggg
     2041 cccgaacccg gccccccgcc gccgtggggc gtggcgaacg tcttgtggag gttcaagtgg
     2101 aggacgtcga cgccgaaatc gcccggccgg gcgattcccg ccagggcgtt catgttcgcc
     2161 ccgtccatgt agagaaggcc gcccttgccg tgaacgatgt cggcgatctt gcagatgtcc
     2221 gattcgaaga cgcccagggt gttcggattg gtgaccatca gggcggcaac ctcgtcggtc
     2281 atggcttgtt ggagggatcc caggtcgatc cggccggcgg cgttcgattt gatctcccgg
     2341 acctcgtagc cactgaggtg ggcgctggag gggttggtgc catgggcgga atcggggatg
     2401 aggacgatct tgcggggatt gccgcgggac tcgtggcagg cccgcgccag catgattccc
     2461 gtgagctcgc cctgggctcc ggccgaaggc tggagggtga cggcgtcgaa tccggtgatc
     2521 tcgcagagaa gccgctccgt ccggtaaagg atctcgagat ttccctgaat cgtctcgacg
     2581 ggggccaggg ggtgaacctc ggcgaatccc ggaaggccgg cgattttttc gttgaccttg
     2641 ggattgtact tcatcgtgca ggaccccagg gggtagagcc cgagatcgac acagtagttc
     2701 tgctgggaga gacgggtgaa atggcggacc acctcgactt cggaaacctg gggaaagccg
     2761 gggatgccct cccgaactgg aagcccggcg agggcttcgg ccgcggccgg aacatccagc
     2821 ttggggaccg cgtagccttt tttgccgggg tcgctgattt cgaaaatgag cggttcgcgg
     2881 atcatcggag gacctcctcg agggcggccg ccagtccgtc gatttcctct ttcgaccgga
     2941 cttcggtgac gcagaccagc atcgtcccgg gaagctcggg atagagcgga gcgagctcca
     3001 ggccggcgag aatgcccttg gcccggaggg ccgcggccgc ggcggccgcg tccttgggaa
     3061 gctggaggac gaactcgttg aatgtttttc cggtaaagcg gaggcggatg ccgccgacgg
     3121 cggcgagctt gccggcggcg taggccgcct tccggacgtt ctgcaaggcg atgtcccgga
     3181 ggccgttttg gccggccgat tcgaggtaaa tcgtcgcccg gagggcgcac cagccctggt
     3241 tgctgcagat gttcgacgtc gctttctccc ggcggatgtg ctgttcccgg gtcgagagcg
     3301 tcaggacgaa gccccgccgg ccctcgacgt ccttggtcaa gccgcagatg cggccgggca
     3361 gctggcggag atactctttt ttgcagccca tgaacccgag gcccggaccg ccgaaggacg
     3421 gggggatgcc gaacgactgg gcttcgccgc agacaatgtc ggcgccgagc ttgccgggcg
     3481 cctcgagaat cccgagggaa agagcctcgg ccacggagac gatcagcagg cccttggact
     3541 cgtggacggc agccgcgcag gcggccagat cctcgaccac gccgaagaag ttgggggatt
     3601 ggacgatcaa gccgcagacg tccgggcccg ttttcgcccg gagctcctcc ggattgaggc
     3661 cgccgtcccc gccgaatccg acttcctcca cggccaatcc gaggttccgg gtcagcgttc
     3721 ggactgtctc ccggtactgg ggatggaggg aacgggcgac gagaatccgg ctccgaccgg
     3781 tcaggcgccg ggccatgagg gcggcttcgg ccgcggccga ggcgccgtca taaagggaag
     3841 cgttggcgat gtccatcccg gtcagacggc agatcagggt ttggtactca aagatgacct
     3901 ggagggttcc ctggctgact tccggctgat agggcgtgta gggcgtgagg aactcggtcc
     3961 gggcgctcag ggaatcgacg accgcgggga tgtaatggtg atacgtcccg ccccccagaa
     4021 acgaccggtg ccgcccgtag gtatttcggc cggcgacagc gtccatgtgg ccgagcagct
     4081 ccggctcgga catcgcgggg ggaaggttga gatcccggcg cagccgcgaa tcctcgggga
     4141 tgcagccgaa cagctcctcc accgaggaga cgccgaggcg ggccagcatc tccttcttgt
     4201 cttgatcact caacgcaata tagctcatga accggccttt ccaggaggat taatgttcca
     4261 agccctcgag atatttctca taatcggcgg cgttcatcag ggcattcaga tcccccttgt
     4321 ccgccatttt cagcttcagg atccaccctt ggccgtgggg gtcctggttg accttttccg
     4381 gggcgtcggt caagccggcg ttcgtctcga tgacttcgcc ggaaacgggc gaaaagatct
     4441 ccgacacggc cttgaccgat tcgatcgagc cgatcgtctg gtgggcggtc agcttggtcc
     4501 cgacggccgg gagatcgacg taaacgacgt cccccaggtg attctgggca tagtcgctga
     4561 tcccgatgac ggcggtgtcg ccgtcgacct tgacccattc gtgctccttg gagaacttgt
     4621 aatcttgagg atacatgcgc ggcctccttc tgttatggat aatgtcggcg gatgagactc
     4681 agtaatcccg cttatagaac ggcgagggga cgaccttggc ccgggctttc ttgtcccgga
     4741 tcaggatttc gaactcggtc cccacggcgg ccttggccgc cggcagatag accgttccga
     4801 tgcttttctt aagaagcggg gcgaacgttc ccgagcagac ctgtccggcc ggggctccct
     4861 cgacgaagac ggggtaatgg gcccgggcga tgccccggtc gatcagctcg aagccgacga
     4921 gcttgcgggt gatcccctcc tcgttctgcc gggccaggac gtcgcggccg atgaaatcgc
     4981 ccttcttgaa cttgacgatc cacttgaagt ccgcctcgag cggtgtcgtc gtgtcgtcga
     5041 tatcgttgcc gtagaggggc aggcgggctt cgaggcggag cgtgtcccgg gcaccgagcc
     5101 cgaccggctg catcccgagc ggcgtcccct tctccatgag ggaatcccaa aggattccgg
     5161 gatcgggatc gagcgtgtag atttcgaacc cgtcctcgcc ggtgtagccc gtccgcgaga
     5221 ccaggcattc cttgccggcg accttgccga aggcgaaggt aaacggcttc atcccggcca
     5281 gggggatctc ggtcatcggc tggaggatcg ggatcgcctg gcggccctgg agggcgagct
     5341 ggctgtagcg gtcggaggcg ttgtccagga cgacgtcgta cgcgccctca tgggcggtga
     5401 gataagcgaa gtccttgtcg gtattggccg cgttgacgac gagcaggaag aactccggat
     5461 ggaggcaata aatcagcatg tcgtcgacat aggtcccctt gtcggtcatg aggccggagt
     5521 actggatcat cccgggggaa aggcgggctg cgtcgttgga cgagacatgc tggatgaagg
     5581 ccagggcgtc ttttcccgtg acgtggacct cacccatgtg actgacatcg aagagcccgg
     5641 ccgccgtccg gcaggcgacg tgctcgtcga tgattccctt gtactcgacg ggcatgtccc
     5701 agccgaagaa atcgaccatt ttcgccccga ggcggcgatg atgagcgttg aaacgggttt
     5761 gtttcatcgg accttccttt tggaaatcag agatcgagaa tgagagatgt tttttttatc
     5821 acaacggccc cgtcttggca aggggaggac gagagcgtac aatgccgatc aggagaggag
     5881 gagaggccat gaagaatccc gtcgaaatcg ccaaggacgg agctatttat gccatcctgg
     5941 tccgggccga atcggccggc cgcggtccgc gttttttcac cccggacgat ttctcccagc
     6001 agctcggtct cctcgtccac cccaagggcc ggaccgtcga gaggcaccgt cacaaggccg
     6061 gcaagggtcc ggtcgtccgg acccaggaag tcctggtcgt tctcgacggg aaaatgcgcc
     6121 tggacctgtt tgacgaatca gggacgaagc tcaaggcggt catccttaaa gccggcgaca
     6181 gcatcctcct ggcccggggc ggccacaaag cgactttctt gaccgattcc cgcatcctgg
     6241 ccgtcaagca gggccccttc gccggccccg gcgaaaaaga attttactag tccgcggctt
     6301 gcgccgtgtg atattctctt tctgaagtgg gcgaaacatc cgttctgacg accctggttt
     6361 tcatcctggc ggcggccctg ttcgtcgtgg ccgtcagcca gaagctgaaa atcctggccg
     6421 tcgtcggctt catgttaacc ggcgtcttcc tgggtccctc gggcgtcggg ctggtcaaaa
     6481 gcgacgcgac gatctccctt ctggccgacg tcggcgtgat gatgctcctg ttcgtcatcg
     6541 gcctggactt ttccctccgc gccgtggtcg ccgcccgccg gccgtttttc ctcggcggcg
     6601 ggcttcaggt cggcctgact acgggagccg tcgccgcgat tttcatcgcc ctgggcttcc
     6661 gcccgggcga ggcggtgatc atcggcttca tcgcggccaa cagcgccacc gctttagtgg
     6721 cgaaaatcct ggccgaccga ggcgaattgg actctccgca cggccggacc atcatggcca
     6781 tcaccctgtt caacgatctg atcgccgtgc ccgtcatcgc cctgattccg ctgctggccg
     6841 gccgagccgc cgggtcgaca ggggtgatcg ccaagttcgg ctggagtctg gcggccatca
     6901 ccgcggcttt tttctttttg cgggcggccg tgccgcggct gctggaggtc attattcgga
     6961 cgcggatcag ggagcttttc attctgtcct cgctcctcct ctgcctggga ctggctctct
     7021 tctccggagc tctggggttg tccatggcct tcggggcttt tttagccggc ttgatcgtcg
     7081 ccgactcgcc ctattcccac cagatggccg ccgacatcct ccctctcaag gacgtcttca
     7141 tcagcctgtt tttcatctcg atcggcctgc tcctcgacat ccggattttc agccgggcga
     7201 tgggccttgc cgcagccatg gccgtcgggg ttattattct caagacccta atcaattcgg
     7261 tcgtccttaa aatcctgaag ctgtccccgc ggggcatcct tctcggatcc ctgggagtgg
     7321 cccagatcgg ggaattcgcc ttcgtcctgg ccggagccgc ccgggcccac ggcttcctcg
     7381 ccggcccggg atttcagctg ttcctgtcct cggcgatcct gaccatgatc gtctcgccgt
     7441 atctcctgag ggcggccgac gccctgagcg ccggggcggc ccgcaaagcg ctgcggaagg
     7501 ccgacggcgc ggcggaggaa aaggaatcgc cggcggccgg gatgaacggc catgtcatca
     7561 tcgtcgggta cggactgaac gggcaaaacc tggcccgcgt cctccgaacc gccgggatcg
     7621 agcatcttgt catcgatctc aatccgaagc ttatccgcca agccgtcgag gacggcgaaa
     7681 aaaccctttt cggggatgtc tgccggacgg agatcctcag gctggccggc atccgccggg
     7741 cgaaaatcat cgtcttcgcc atctccgatg cgaccgccac ccgccgaggc ctgctcctcg
     7801 tccgggggct gggctccaag gctcagatca tcgtccggtc gcgatacgtc accggcctcg
     7861 acgcgctcta cgaagcggga gccgacagcg tgatccccga ggaattcgag acctccatcg
     7921 agattttttc ccaggttctc gaggcctacc acatccccaa aaacatcatc gacgccgagg
     7981 tcaaggccgt ccgggccgaa cggtacggga tcttgcgggc gaccggcccg gtccggccgt
     8041 ccttcgagaa aatcgtcgac ttgctgacgg ccgggacggc tgagacgttt tacgtcggcc
     8101 cggattgccc ggccgccggg cggaccttgg cccagctcga cttgagggcc cggacgggag
     8161 cgaccgtcat cgccgtcgtc cgcggcgaag aatcccacac cggcccggcc gccgatttcc
     8221 tggtcgagcc gggagacacg ctcgtcttag tcgccgatca cgcggccatg gacgtcgcct
     8281 tcgactacct ggccgcccgg gcggcccggg aagccggcgt ccctccggcc gggagttttc
     8341 ctccccggag ctgaaatccg gccggctcac tcgtcgaagg cgccggtgtc gatccaatcc
     8401 ttcttcaaga cggaccagaa atcctcggcc aggccgcgga ggacgtaggc gtacggcagc
     8461 cagccgtacc cgccgtcccc ccatcccttt ccccaggaat tgcggatgag gagggctccg
     8521 gtcgtttctc ccccgccgtt tttttcgttc ctgatcttga gccggtcgtc ataaccgacc
     8581 gcggccacgg cgtgccctcc ctcgatccgc tcccgcggtc ccgggaaggg aatcgccccg
     8641 tcgccgccgg ccgcctcgat ggagccgtag acggtgaatc cgaacatcga cggatgaccg
     8701 gcggccagat gggtcttgac tcggtcgagc gtttcaccgg ccggcgttcc ggggacatcg
     8761 tgacggtaat agcggatggt cttgtagttt tgggcgaaag cgtagcagaa ggccggcggc
     8821 tcgcggtcga accggtccgg ccggtcatcg tagggccagt agtcctcggg aggaacgccg
     8881 aagaggacca tcgctccgat cgtcgtccgg agataagcgc ccgtgtcgcc ggagatcttc
     8941 atcaggttgc gggcggcttt atagaggaag aggcgcgaag cctccaggtg ccggccgaag
     9001 gcccggcgct cgtaatactc gatgatcccc gttccggcgt tggccgtgca cgagccgagg
     9061 tttccctggt cctcgaccgg cgagcagtgg cttcgcaaat cgacggccgg gggaagcggt
     9121 tcggacgccc ccgcgggccg gcctcccccg cccgcccgct gcggcggaag gcccggccgt
     9181 cgacgggccc cggccatgag gtcccgcacg accggagtct cccccgaata atcccggaag
     9241 tccgggatgt ccggtatcca gccgagccgt tgacggatca tgtctccctc ctaaggcggc
     9301 cttccgcccg ttaagtcgaa atccggggga aaaggtggga aaaccgcctt tgtttttcaa
     9361 ttcttaattc cggattcgat ttcccgtata atagcgcctc ttcgggccgg agcgcgggga
     9421 gaacgagaat cattggccgg cctcaacgat tccggaggag gaagagcgat gaagtcctgg
     9481 cgcgtgaagg cggtcttggc ggccgcggct ctgacgatcc tgtccggggc ggtcccctcg
     9541 tccgcccaat tcgaattcca ggcggacgtg atgtccaagt acatctggcg gggctgggac
     9601 ctttattgga acaaccagcc cgccctccag ccgtccctga cctacgcgtt cggggaatcg
     9661 ggattttccg tgaacctctg ggggagcttc gccctgggcg aacggccggt ctacaaggat
     9721 tacgatgaaa tcgacttgac tttggattat accttttccc tttcggacca tctggaaatg
     9781 tccgccggat tcatcaatta cggctatttt ttcggcggga aggacgtctg caaggagaac
     9841 acgacgcagg aattctacgt ctcggccgga tggcccgaaa tcctgttgtc ccccaccctc
     9901 accctcttct atgacttcaa cctcggctcc ggcctttacg cgcttttcga ggcctctcac
     9961 tcggtggcga tttcggagag cctcgggctg gatttgtgcg ccgaactcgg ctacaacagc
    10021 cggcagttca ttgaagagaa cggattttcc gacctgacga tcggcgcctc gctgcccttc
    10081 accctcggag actggacgat cgcccccttg atccgctaca cgttcgtttt tctcgacgcc
    10141 gtcaacgagg acgacgagct ctggttcggg atctccctca tccggtaagc cggctcagga
    10201 tttgacctcg acgaatccgg ccttgctctt gaggccggcc acgaaatttt cgtagacgcc
    10261 ggcccgcttg gccagcttta aattgtcttc gagccgggtc cggaccgctt cgaacggttc
    10321 ggtttccttc atccggttct cgaccttcag gatgtgaaag ccgtatttcg tctcgatgac
    10381 gccgctgagc tcccccacgg gcacgctgaa ggcggcgtcc tcaaacggct tgaccatcat
    10441 tccccggggg aaatcctcat aaagcccgcc gttttccttg gaaccgggat cctcggtata
    10501 ttcccgggcc agggcggcga aatcctcccc cttccgcgcc cgggccagga ggtcttccat
    10561 ctttttccgg atctccggaa tctcggactc gggtttgccc tgggtcatga ggaggatgtg
    10621 ccggacggtc gcggtcttgt ccgcggcgta agcctggcgg agctcctcct cgccgaccgt
    10681 gatcttctgg aaaacatcgg cctcgaggaa cttgccgatc atctgctcct cccgcatcat
    10741 cctcttgaag gactcgggat ccaccttgtt cgacttcagc atctcgagga acgcctcctc
    10801 gccgccggcc ttggcatatt gctcggccag aaccttgtcg aattcctcct gcgagaaggt
    10861 gatcccggcg gcctcggccg cggccgtcag cagccggcgc tcgccgatct gctcggcggc
    10921 ctggaggacg gccgctttca agcccgccgg atcccggtcg atgagctgaa aagccgcgtt
    10981 ccccatggtg ttcagga
//
LOCUS       DAOOXH010000082        10886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_6480_length_10886_cov_6.518696, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000082 DAOOXH010000000
VERSION     DAOOXH010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 10886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10886
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6480_length_10886_cov_6.518696"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="PKV64_09100"
     CDS             complement(<1..1015)
                     /locus_tag="PKV64_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HPN17271.1"
                     /translation="MIFQIVLILLAMIGAYAAAHWKKLPVEICIFLTAVAGGLAGAVV
                     KTPPLLELPRHLVEGSFPYLDVMLVFSTATIFMAVVEESGGVAYVVRGTIRLFYNVRV
                     LALLVLMIIVLIPGALTGAGSVSLLVVGAPVALALKHLGITERRVAAILFIVAGLAAV
                     APPVNIWAMMLCAGTAIPYVGFTYVLGIPVLVLGTITMLTLGLKRVGASDKQAALAAL
                     PEVPAKMTGWRVLLPFLVFFGFIAVSRIWPFETPVIGLPLQFTASALVALLVSPKRID
                     FLALSRKTMIRLLPLLATTVVVGMLQQIMTASGVRGLLSFAVISTPLILLYILLAIII
                     PFSE"
     gene            complement(1098..3239)
                     /locus_tag="PKV64_09105"
     CDS             complement(1098..3239)
                     /locus_tag="PKV64_09105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPN17272.1"
                     /translation="MTATIRKTSGFPGFLFLFVLVFGGAWAAQAPAAQGAGEGQDLLL
                     RHREWLKITDTFILPAEKEAFLSLTNERDRDMFMEAFWKQRDPRPDTPRNEFREEIEK
                     RFRFVMENFGRLALREAWQTDQGRIYMILGPPQSKDTYEGTSGLHPATIWHYIGDPLK
                     KLPQAFDLVFFQRGGGGEFRLYDPISDGPASLLVDTRQFNRNNPIEVFQKIKDIAPPL
                     ARAALTIVPNQMPSNYRPTPQNTMIIADIFASAKRGISTAYVNHFLNYRGIVGPEYLA
                     NFIESDASASLVRDGGRGMTFLHFAILPRHSSFDYDPSSDQYGCDFKLTVGLKSGETT
                     VFQYVRDYPFRFPAGQVENVRKNGIAVQDIVPVAQGKFKLTVLLQNAVGKEFSLFEKS
                     LDVPAETGPAELTEPFIGYGLQEAPEPDYAPYKLAGRRILTDPRATINRADTLVYGLS
                     VLRLPPDLWAGGTVDVAAAGTMSGGKPVTLRTIKLSETEYSPTLTITGETPAVDLRPD
                     YYELTFSLKNAAGRVLAATEANFILSPVAEVPRAVILHSTVPESRAFLFDYGLAVQYD
                     QSGDAARAEEAYRKIQRDHPEFEEGMFRFANFLVRAGNNTEALEIVELFAASSNFRFD
                     YFLIKGLALEEKGAWEEALANLLEARKLYGGDTRVLNALGGCYYRTGKKAEAREALNA
                     SLRLASGQAEVRALLERIEKERPEDPPTAFD"
     gene            3478..4809
                     /locus_tag="PKV64_09110"
     CDS             3478..4809
                     /locus_tag="PKV64_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN17273.1"
                     /translation="MTPRLGPHQPIPQDDKRTIKGWVRYDWANSVYQLTISSAIFPIY
                     YNQVTRHGDDFTVHFFGLPVINTVLYSWAIAAAYLLVAFLSPFLSSIADYTGLRKGFM
                     KGFTILGAASCSALFFFDSNTIELGVICFALGTVGYGGSLVFYNSFLPIIASPAEQDK
                     VSARGYISGYLGGVVLLIVNLIVVMQPAFFGITDPKLPAKLAFLSVGLWWFGFSQTTF
                     RRLPKYTFGHRETGTSVILGGYRQLRIVFRQIRRMPRLRIFLPAYFFITMGLLTVMFM
                     AATYGEKALRLGETVLIATILLIQLIGMLGAWLFARLSGRVGNIKAFRLTVYIWIGIC
                     VAAYFITDAVGFLCVGIAVGLVMGGSQSLARSTYSKMLPPTEDHTSFFSFFDVMEKLA
                     TVAGTFSFGIIEALTGNMRNSVLAITLFFAVGLAFLFRLMVVERRPAAAES"
     gene            complement(4934..6391)
                     /locus_tag="PKV64_09115"
     CDS             complement(4934..6391)
                     /locus_tag="PKV64_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPN17274.1"
                     /translation="MKNIAVPLALLLAAAVAPGAADIRHLSPELQKKVGETDYASVRI
                     KNFPFASEAWTFRKFTFFETVAKLKELGVRGLEAFPGQALAAEFPGARFGEAMTPEQI
                     AKTKDVLAAAGVTLYGYGVVDIGRTEAEMRKVFDFARTMGIRILVCEPEDDDFTLLEK
                     LVAEYDIKIAIHNHPAPSKYHLPETVFRHVDGKDPRIGSCADNGHWMRGMIDPREAFK
                     LLEGRILDVHLKDRNGFGTGPGVDDVAWGAGAGTIRDLLAELTLQDYDGWLTMEYENE
                     AEVDDPMPAFRKSIDYVKSITYYDGYTQIFRRGWGGDYEKHGWNHYGPGHFEVDPKAG
                     VLKGQGGMGLLWYGKKVKDFILDLEFKCAAETTNSGIFLRVPNVPVSDDYIYHSIEIQ
                     IDDHSTGLHHTGAAYDIQAPLTGATLPPGRWNHMKVEFRGSRLNVWINEKQVLDWEAA
                     PGGKVRDISPEGYIGLQNHDSDSPPYFRNIYLKEL"
     gene            complement(6414..7676)
                     /locus_tag="PKV64_09120"
     CDS             complement(6414..7676)
                     /locus_tag="PKV64_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN17275.1"
                     /translation="MSHHSAKPNLGVGIIGGGFIARFHIKSFVGVRDADILGVFDPDR
                     KRAREACALARSLGVGEAKPYASITAMAADPAIGALWICAPNFTRLEVMEEIAHAVLS
                     GKAELRGVACEKPLGRNAKEARRMLALVEKAGILDGYLENQVFSPAVTRGKEILWARG
                     AAIAGPPYLARAAEEHSGPHMPWFWEGTLQGGGVLNDMMCHSVEEARFMLTPPGAPRD
                     VLTPVSVTAYANCLKWQEPKYAAVLKKNSGGKTDYLNRPAEDFARSLVEFRNKDGKIL
                     VVETTTSWCYVGAGLRLSMELLGPEYALQINTLDGALKVFFSREVRGKAGEDLVEKQN
                     AEIGLMPVVADEAAEYGYTHENRHMVQSFRRGVRPEENFADGAAVAELLMAAYMSAET
                     GKTVPFPPKGLGTFVPAVAKGTWNPIKK"
     gene            complement(7695..7871)
                     /locus_tag="PKV64_09125"
     CDS             complement(7695..7871)
                     /locus_tag="PKV64_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17276.1"
                     /translation="MIIPPLIGGRSAGEAAPTIDAAKTFLVLKARAVLCPEMIQAFFR
                     DKKAAGKIPGPTAL"
     gene            8303..10285
                     /locus_tag="PKV64_09130"
     CDS             8303..10285
                     /locus_tag="PKV64_09130"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPN17277.1"
                     /translation="MRKRAIRALAVSLAVIGAVSLHPPRAAAKDFYFPEVRIDIAVEA
                     DGSFTVDENRTYAFDGEFSWATLWIPLRVERMGRTYEAEIEDFAVFEDGTPLRTEQGG
                     SRDRLEAKWYYRARDERKTFSIHYRVRGGIRTSGEATELYWQPIGSGWDKPASLAVVT
                     VSLPEPVSGASELLVYGHGPLSGRSEIVDLQTARFTVPNLPAGRMVEIRMVWPAGIVS
                     GLPDAGLTFASVRAEEERFVDETIARASAAQAEAARRTRRTERLVQAYLFGLIALPIL
                     WLPFFMRTWRAVGKDYAFTDIPDYFREIPSDLPPALVDLLQREGISVAPAAFTATIFD
                     LARRGFLEIEDRTEEKTGFFGTKQKTVTRFTFKKEFGGEDDGLRPFERDVLGFLAAAA
                     GGAVDNTGAAALAAALGSAAKALERFRRARGGAVSPPLPVDPGLTSPVSPVGTVFSLD
                     ELKSWLKKNPAKFQAWFRAWGEAVKAEGKALAFIEPESLKRRNRFIAATVPAAVLTVN
                     PVLGILGAVLIPKIKRRSLRWARENEMWKALRRFLDDFSEFKETPPEAYRLWEQYLVF
                     GILFGNAKKILKSLPLILGDERAAVPAWYAGASRQAFLSGGGVGSIASMVRSIESAAT
                     SIQQASTAAAHYSSGGGGGFSGGGGGGGGGGGGSAG"
     gene            10334..10603
                     /locus_tag="PKV64_09135"
     CDS             10334..10603
                     /locus_tag="PKV64_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HPN17278.1"
                     /translation="MSENTNPKTKTRTEEFKASGGDILNKIKELLHEGNIRRIILKDE
                     QGKTFMEIPLTVGLVGVALLPVLAAIGTVAALVSNLTIVVEKVED"
     gene            10680..>10886
                     /locus_tag="PKV64_09140"
     CDS             10680..>10886
                     /locus_tag="PKV64_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17279.1"
                     /translation="MRPPRIIPRFFILSLIGVAATHAAADHRFLNLMPRPSAVLLTEG
                     RFHLSETFTVGALGQPGDRARRAAD"
BASE COUNT         1874 a         3338 c         3596 g         2078 t
ORIGIN      
        1 cctcggagaa ggggatgatg atggccagga ggatgtacag caggataagc ggcgtgctga
       61 tgaccgcgaa cgaaagaagt ccccgcactc cggaggcggt catgatctgc tggagcatgc
      121 ctacgacgac cgtggtcgcc agcaggggca gcagccggat catcgtcttt cgggagaggg
      181 ccaggaagtc gatcctcttc gggctgacca gcagggcgac gagggccgag gccgtgaact
      241 gcagcggcag gccgatgacc ggcgtctcga acggccagat ccgggagacg gcgatgaacc
      301 cgaagaagac gaggaacggg agaaggacgc gccatcccgt cattttcgcc gggacttcag
      361 gcagggcggc cagggcggct tgcttgtcgg acgcgccgac ccgtttcagc cccagggtca
      421 gcatggtgat ggttccgaga accaggacgg ggatgccgag aacataggtg aagccgacat
      481 acgggatggc tgtcccggcg caaagcatca tcgcccagat gttgacgggc ggggcgacgg
      541 ccgcgaggcc ggcgacgatg aatagaatgg ccgcgacccg gcgctcggtg atgccgagat
      601 gcttgagagc cagggccacc ggggcgccga cgacgagcag cgacaccgac cccgcgcccg
      661 tgagagcgcc ggggatcagg acgatgatca tcaggacgag cagggccagg acccggacgt
      721 tgtagaagag ccggatggtc cctcggacga cgtaggcgac gccgcccgat tcctcgacga
      781 cggccatgaa gatcgtggcc gtcgagaaga ccagcatcac gtcgagatag gggaaggagc
      841 cctcgacgag gtgacggggg agttccagga gaggaggagt tttcaccacg gcgccggcga
      901 ggccgccggc caccgcggtc agaaaaatgc agatttccac cgggagcttt ttccagtggg
      961 cggcggcgta agcgccgatc atcgcgagca gaatgaggac gatttgaaaa atcatctcgc
     1021 ggctccttcc gccgtcgggc ggttgagatc ctgaattcag tcgagcttga agatatatcc
     1081 catgcccagc gggaatgtca atcgaatgcg gtcggcgggt cttccggccg ttccttttcg
     1141 attcgttcca gcagggcccg gacttcggct tggccggaag cgaggcggag ggaggcgttg
     1201 agggcttccc gggcttcggc ttttttcccg gtccggtaat agcacccgcc caaggcgttg
     1261 agcacccgcg tgtcgcctcc atagagcttc cgggcttcga gaagattcgc cagggcttct
     1321 tcccacgcgc ccttttcctc gagggcgagg cccttgatca ggaaataatc gaaccggaag
     1381 ttggacgagg cggcgaagag ctcgacgatt tcgagcgcct cggtgttgtt cccggcccgg
     1441 acgaggaaat tcgcgaatcg aaacatccct tcctcgaact ccggatggtc ccgctggatc
     1501 ttccggtagg cctcctcggc tcgggccgcg tcgccggatt ggtcgtattg aacggccagg
     1561 ccgtaatcga agagaaacgc ccggctctcc ggcaccgtgc tgtgcagaat gacggcccgg
     1621 gggacttcgg ccacggggga gaggatgaag ttggcttccg tcgcggcgag aacccggccg
     1681 gcggcgttct tgagggagaa ggtcagctca taatagtcgg gccggaggtc gacggcgggc
     1741 gtttcgccgg tgatcgtcag cgtcgggctg tattcggttt cggacagctt gatcgtccgg
     1801 agggtgaccg gtttgcctcc gctcatggtc ccggcggccg cgacgtcgac cgtcccgccg
     1861 gcccacaggt cgggcggcag gcggaggacg gaaaggccgt agaccagcgt gtcggcccgg
     1921 ttgatggtcg ccctcggatc ggtcaggatc cggcggccgg cgagcttgta gggggcgtaa
     1981 tccggctcgg gcgcttcttg gagaccgtag ccgatgaagg gctcggtcag ttcggccggg
     2041 ccggtttcgg ccgggacgtc aaggctcttt tcgaagagcg aaaactcctt tccgacggcg
     2101 ttctggagaa ggacggtcag cttgaacttg ccttgggcga cggggacgat atcctggacg
     2161 gcgattccgt tcttgcggac gttctcgact tgcccggccg gaaatcggaa gggatagtcc
     2221 cggacatatt ggaacacggt cgtctcgccg cttttcaagc cgacggtgag cttgaaatcg
     2281 cacccgtact ggtcggagga cgggtcgtaa tcgaaggacg aatgccgggg cagaatggcg
     2341 aaatggagga aggtcatccc ccgcccgccg tcgcggacga ggctggccga ggcgtcgctt
     2401 tcgatgaaat tcgccaggta ttcggggccg acgattcccc ggtagttgag gaagtggttg
     2461 acgtacgccg tgctgatccc ccgcttcgcc gaggcgaaaa tgtcggcgat gatcatcgtg
     2521 ttctgggggg tgggccggta attggagggc atctggttgg ggacgatggt cagggcggcc
     2581 cgggcgagcg gcggggcgat gtccttgatc ttttgaaaga cctcgatggg attgttccgg
     2641 ttgaattggc gggtgtcgac caggagactg gccgggccgt cggagatcgg atcgtacaga
     2701 cggaattcgc cgcctccgcc gcgctggaaa aaaaccaagt cgaatgcctg cggcagcttt
     2761 ttgagcgggt ccccgatata atgccagatc gtggccgggt gcagccccga cgtcccctcg
     2821 taggtgtcct tggactgggg cgggccgaga atcatgtaga tccgcccctg gtccgtctgc
     2881 caggcctcgc ggagagccaa ccggccgaag ttctccatga cgaaccggaa gcgcttctcg
     2941 atctcctccc ggaattcgtt ccggggcgtg tccggacggg gatcccgctg cttccaaaat
     3001 gcttccatga acatgtcccg gtcgcgttcg ttcgtcaagg agaggaaggc ttccttttcg
     3061 gccgggagaa tgaaggtgtc ggtgatcttg agccattccc gatgccggag gagaaggtct
     3121 tgtccctcgc cggcgccctg ggccgcgggc gcctgggcgg cccaagcgcc gccgaagacg
     3181 aggacgaaaa gaaaaaggaa accgggaaat ccggaagtct ttcggatggt cgcggtcatg
     3241 actgaacatc cctgtcgttc aaatcgcggc gcgcggccgc gcgtcgccgc cggctttgga
     3301 ttgtagcatc ctccccggag gaatgccaag gacgcgggct tgggccggga gacgtcttcc
     3361 tcgaaaggat ggagggaacc ggcgacgcgg attttcccgc cgggcggccg aagttgcatt
     3421 ttcggccgag tttcattata aataggcgct ttcaattccg tcaggaaaag aggatccttg
     3481 actccacgcc tcggcccgca tcaacccatt ccccaggacg ataagcgcac gatcaaaggc
     3541 tgggtccggt acgactgggc caactcggtc taccagttga ccatctcctc ggccatcttc
     3601 cccatctact acaaccaggt gacccgtcac ggcgacgatt tcaccgtcca tttcttcggc
     3661 ctcccggtca tcaacaccgt cctctattcc tgggccatcg ccgcggcgta cctcctggtc
     3721 gccttccttt cgccctttct ctcctcgatc gccgattaca ccggcctgcg gaagggattc
     3781 atgaagggat tcacgattct gggcgcggct tcttgctcgg ctctgttctt tttcgactcg
     3841 aatacgatcg agctcggggt catctgcttc gccctgggca cggtcggcta cgggggcagc
     3901 ctcgtctttt acaactcctt cttgccgatc atcgcctcgc cggccgaaca ggacaaggtc
     3961 agcgcccggg gatacatctc cgggtatctg ggcggcgtcg ttctcctgat cgtcaacctg
     4021 atcgtcgtga tgcagccggc cttttttgga atcaccgatc cgaagctccc ggccaagctg
     4081 gccttccttt cggtcgggct gtggtggttc ggcttttccc agaccacgtt ccggcggctg
     4141 ccgaagtata cgttcggcca ccgggagacg ggaacgagcg tcatcctggg cggctaccgg
     4201 cagctgcgga tcgtattccg ccaaatccgt cggatgcccc ggctccgcat ttttctcccg
     4261 gcctactttt tcatcaccat ggggctgctg acggtcatgt tcatggccgc gacctacggc
     4321 gaaaaagcgc tccgtttggg cgaaaccgtc ctgatcgcga cgatcctcct catccagctg
     4381 atcggcatgc tgggggcctg gctgttcgcc cggctttcgg gccgggtcgg caacatcaag
     4441 gccttccgcc tgacggtcta tatttggata gggatttgcg tcgccgccta cttcatcacc
     4501 gacgccgtcg ggttcctctg cgtcgggatc gccgtcggcc tggtcatggg cggctcgcaa
     4561 tcgctggccc ggtcaacgta ttccaagatg ctgccgccga ccgaggacca cacctcgttc
     4621 ttcagcttct tcgacgtgat ggagaagctg gccacggtgg ccggcacctt cagcttcgga
     4681 atcatcgagg ctctgacggg caatatgcgc aactcggtcc tggccatcac cctgttcttc
     4741 gctgtcggct tggcttttct tttccggctg atggtcgttg aacgccggcc ggcggccgcg
     4801 gaatcctgaa gaaaccggcc tcagaggaag acaccatccc tcttcacctc atttctccct
     4861 ccgctcccgt gaatgaaagg agttccgggc acaaggggaa atcgggtgaa tcgggatggt
     4921 gtctcctctt tgtttacagc tctttgaggt agatgttccg gaagtacggc ggcgagtcgc
     4981 tgtcgtggtt ctggaggccg atgtacccct ccggggagat gtcccggacc ttgcccccgg
     5041 gcgcggcctc ccaatccaaa acctgttttt cgttgatcca gacgttcagg cgcgagcccc
     5101 ggaattccac tttcatgtgg ttccaccggc ccggcgggag cgtcgcgccg gtcaaaggcg
     5161 cctggatgtc gtaggccgcc ccggtgtgat ggaggccggt cgaatggtcg tcgatctgga
     5221 tttcgatcga atgataaatg tagtcgtcgc tgaccgggac gttggggacg cggaggaaaa
     5281 ttcccgagtt cgtcgtttcc gcggcgcatt tgaattccag gtcgaggatg aaatccttga
     5341 ccttttttcc gtaccagaga aggcccatgc cgccctggcc cttcaggacg ccggctttcg
     5401 gatcgacctc gaaatgcccc gggccgtaat gattccagcc gtgcttttcg tagtcgccgc
     5461 cccagccgcg gcggaagatc tgcgtgtagc cgtcgtaata cgtgatgctc ttcacgtagt
     5521 cgatgctttt ccggaaggcc ggcatcgggt cgtcgacctc ggcttcattc tcgtattcca
     5581 tcgtcagcca gccgtcataa tcctggaggg tcagctcggc caggagatcg cggatggttc
     5641 ccgcgccggc gccccaggcg acgtcgtcga cgcccggccc cgtcccgaaa ccgttccggt
     5701 ccttcaggtg gacgtcgaga atccggccct cgaggagctt gaacgcctcc cgcgggtcga
     5761 tcatcccccg catccagtgg ccgttgtcgg cgcaggagcc gatgcgggga tccttgccgt
     5821 cgacgtgccg gaaaaccgtt tcgggcaagt ggtacttgga cggagcgggg tggttgtgga
     5881 tggcgatctt gatgtcgtat tcggcgacca gcttttccag cagggtgaaa tcgtcgtcct
     5941 ccggctcgca gacgaggatc cggatgccca tcgtccgggc gaagtcgaag accttgcgca
     6001 tctccgcttc ggtccggccg atatcgacga cgccgtagcc gtagagggtc acgccggcgg
     6061 ctgccaggac gtccttcgtt ttcgcgatct gctcgggcgt catggcctcg ccgaagcgcg
     6121 ccccggggaa ttccgcggcc agggcctggc cgggaaaagc ctcgaggccg cggacgccga
     6181 gctccttgag cttggccacg gtttcgaaga aggtgaattt ccggaacgtc caggcctccg
     6241 aggcgaaggg gaagttcttg atgcggacgg acgcgtagtc cgtctcgccg accttctttt
     6301 ggagctcggg ggagaggtgc cggatgtcgg ccgcgcccgg cgcgacggcg gccgccagca
     6361 gaagggcaag cggaacggcg atgtttttca tggatcctcc cgtttgatgg acttcacttc
     6421 tttatcggat tccacgttcc cttggccacg gccgggacga acgtgcccag gcccttgggc
     6481 gggaaaggaa cggtcttgcc cgtttcggcg ctcatgtagg cggccatcag gagctcggcc
     6541 acggccgcgc cgtcggcgaa gttttcctcc gggcggacgc cgcggcggaa cgactggacc
     6601 atgtggcggt tctcgtgcgt gtagccgtac tcggccgcct cgtcggccac gaccggcatg
     6661 aggccgatct ccgcgttctg cttttcgacg aggtcctcgc cggccttgcc ccggacctcc
     6721 cggctgaaga agaccttcag ggcgccgtcg agcgtgttga tctggagggc gtactcgggg
     6781 ccgagcagct ccatgctgag ccggaggccc gctccgacgt agcaccagct cgtcgtcgtc
     6841 tcgacgacga ggatcttgcc gtccttgttc cggaactcga ccagggaccg ggcgaaatcc
     6901 tcggccgggc ggttgaggta atcggtcttt ccgccgctgt ttttcttcaa gacggcggcg
     6961 tatttcggct cctgccactt gaggcaattc gcgtaggccg tgaccgagac gggcgtcagg
     7021 acgtcgcgcg gcgcccccgg cggcgtcagc atgaagcggg cttcctcgac cgagtggcac
     7081 atcatgtcgt tgagcactcc gccgccctgg agcgttcctt cccagaacca aggcatgtgc
     7141 gggccgctgt gctcctcggc cgcccgagcc aggtagggcg ggccggcgat ggccgcgccc
     7201 cgggcccaca ggatctcctt gccgcgggtg acggcggggg agaagacctg gttttcgaga
     7261 taaccgtcca ggatcccggc tttttcgacg agggcgagca tccggcgggc ttccttggcg
     7321 ttgcggccaa gcggtttttc gcaggccacg ccccgcagct cggctttgcc cgaaaggacg
     7381 gcgtgggcga tttcctccat gacttcgagg cgggtgaagt tgggggcgca gatccagagg
     7441 gcgccgatgg ccggatcagc ggccatggcc gtgatcgagg cgtacggctt ggcttcgccg
     7501 acgccgaggg agcgggccag ggcgcaggcc tcgcgggccc gtttccggtc gggatcgaaa
     7561 acgccgagga tgtcggcgtc gcggacgccg acgaagctct tgatgtggaa gcgggcgatg
     7621 aacccgccgc cgatgatgcc gacgccgagg ttgggtttag ctgaatgatg cgacatgggg
     7681 agatctcctt tcgtttataa tgccgtcggc ccggggattt tcccggccgc ttttttatcc
     7741 cggaagaagg cctggatcat ctccgggcag aggacggccc gagccttcaa aacgagaaat
     7801 gtcttggccg cgtcgatggt cggggcggct tcgccggcgg accgtccgcc gatcaacggc
     7861 gggatgatca tcgccggggg gaagcagggc gaaagaaagg cccggcccgg cgccggagaa
     7921 gcggggatcg ctccgaagac aagccgggag cattggagcc cgcgagcccc ggacggcgca
     7981 ggggaggaac atcatgacgc cgagacggag ttttgcgctc atgcgagaaa accaaagtcc
     8041 tttcgtggga aatcgaatgc ccgcgcggcc tccgctaaaa taatccgaac cggccgccgt
     8101 gtcaatcaat gaggagacac aatgcctaat caccatgttg attatctctt cgctttgccc
     8161 gatccgttcc ctcgttcccg ccgccgatct tccgaaacgg ggacaatggg gcttgcgttt
     8221 ccccttgttt gaaataaggt taacaggtat tgtgtctcct gatttccttt ggtagggtaa
     8281 aaagaggagg gagaacgtga agatgagaaa acgagcgatt cgggcgttgg ctgtgtcgct
     8341 ggcggtcatc ggggcggttt ctctccatcc tccccgggcc gcggccaagg atttctattt
     8401 ccccgaggtc cggatcgaca tcgcggtcga ggccgacggg agcttcacgg tcgacgaaaa
     8461 ccggacgtat gccttcgacg gcgaattttc ctgggccacc ctctggatcc ccctccgggt
     8521 cgagcggatg ggccggacgt acgaagctga aatcgaggat ttcgcggttt tcgaggacgg
     8581 aacgcccctt cggactgaac aagggggctc gcgggaccgc ttggaggcca agtggtacta
     8641 tcgggcccgg gacgaacgga aaacgttttc tattcattac cgggtccggg gcggcatccg
     8701 cacgtccggc gaggcgaccg agctttactg gcagccgatc gggagcggct gggacaaacc
     8761 ggcctccctg gcggtcgtca cggtctccct gcccgagcct gtctccggcg cgtccgagct
     8821 cctcgtctac ggccacggcc cgctttcggg acgttccgag atcgtcgacc ttcagacggc
     8881 ccggttcacc gtcccgaacc ttccggccgg gcggatggtc gagatccgga tggtctggcc
     8941 ggcggggatc gtctccggcc ttccggacgc gggcctgacg ttcgcttccg tccgggccga
     9001 ggaggagcgg ttcgtcgacg agacgatcgc ccgggcctcg gccgcccaag ccgaggcggc
     9061 ccgccggacg agacggaccg aacgtctcgt ccaagcttat cttttcggcc tcatcgccct
     9121 cccgatcctc tggctccctt tctttatgag gacttggcgg gccgtcggca aagattacgc
     9181 gttcacggac atcccggact attttcggga aatcccctcc gatctgcctc cggccctcgt
     9241 cgacctcctc cagagggagg ggatctcggt ggcgccggcg gccttcacgg cgacgatctt
     9301 cgacttggcc cggcgcggct tcctcgaaat cgaggaccgg accgaggaaa aaaccggctt
     9361 cttcggaacc aagcaaaaga ccgtcacccg tttcacgttc aagaaggaat tcggagggga
     9421 agacgacggc ctgcggccgt tcgagcgcga cgtcctcggt ttcctggcgg cggccgcggg
     9481 cggagccgtc gacaacaccg gggccgccgc cttggccgcg gctttgggaa gcgcggccaa
     9541 agccctcgag cgcttccgcc gcgcgcgggg cggcgccgtt tcgccgcccc ttccggtcga
     9601 tccgggcctc acctcgcccg tttcccccgt ggggacggtt ttttcccttg atgagctgaa
     9661 atcctggttg aaaaagaatc cggccaagtt tcaagcctgg tttcgggctt ggggcgaggc
     9721 ggtcaaggcg gaaggaaaag cactcgcttt catcgagccc gaaagcctca agcgccggaa
     9781 ccggttcatc gccgcgaccg ttccggcggc cgtcctgacg gtcaatcccg tcctcggcat
     9841 cctgggcgcg gtcctcatcc cgaagatcaa gcggcgctcg ctccgatggg ccagggaaaa
     9901 cgaaatgtgg aaggcgctcc gccggttcct cgacgatttc tccgagttca aggaaacgcc
     9961 gccggaggcc taccggctgt gggaacaata cctggtcttc ggcattcttt tcggcaacgc
    10021 gaaaaaaatc ttgaaatccc tgccgctcat cctgggcgac gagcgggcgg cggtgcccgc
    10081 ctggtacgcc ggggcctccc ggcaggcgtt cctgtccgga ggaggggtcg gctcgatcgc
    10141 ctcgatggtc cgatccatcg agagcgcggc gacgtccatc cagcaggcca gcacggcggc
    10201 cgcccattat tcctcgggag gcggcggagg attcagcggc gggggagggg gcggcggcgg
    10261 aggaggcgga ggaagcgccg gctgaacttt tccttcttcg tttccgtaga taatcatgga
    10321 aaggacaacg gccatgagcg aaaatacgaa cccgaaaacg aagacgagaa cggaggaatt
    10381 caaagcctcc gggggcgaca tcctgaacaa gatcaaggaa ttgctccacg aggggaacat
    10441 ccgccggatc atcctcaagg acgagcaggg caagacgttc atggagatcc cgctgacggt
    10501 gggtctcgtc ggcgtcgctc tgcttcccgt cctcgcggcc atcgggacgg tcgcggccct
    10561 ggtttccaac ttgacgatcg tcgtcgaaaa agtcgaggat taagcggcct ttttccctcc
    10621 gggccggccc gccggggccg tgttttgcat cccgccggga gaatggatac aatgcgggca
    10681 tgcgcccgcc tcgaatcatc ccccggtttt tcatcctgtc cctgatcggc gtcgcggcga
    10741 cgcacgccgc cgccgatcat cgcttcctca acctgatgcc ccggccctcg gccgtccttt
    10801 taaccgaagg ccggttccat ctgagcgaga cgttcaccgt cggagcgctc ggccagcccg
    10861 gcgaccgcgc ccgccgggcg gccgac
//
LOCUS       DAOOXH010000083        10864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_6499_length_10864_cov_7.139698, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000083 DAOOXH010000000
VERSION     DAOOXH010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 10864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10864
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6499_length_10864_cov_7.139698"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..935
                     /locus_tag="PKV64_09145"
     CDS             180..935
                     /locus_tag="PKV64_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17280.1"
                     /translation="MRPIRSMVFFGLSVLFYSHYALPEENNRLSWRFSAGTSYFASNI
                     VRPLEEAYIEPAISNSHKSLNGPSPAMRLEASYDITRNMFVNLGVEYIFPRTFKQRID
                     LPNEFDEDVLQNDKLSMIPVSLGLGYSILSTARLNVSVIAGADLNIILDSFGRRISGR
                     LQTGASAFGNLHQTNFGFRAGTRIAFDNFFLDISARLFEITLQGDVFMQRTFLLPDQT
                     TYRVVNENFKAGDFWGLDNNPMTVISIGVGFKI"
     gene            1270..1722
                     /locus_tag="PKV64_09150"
     CDS             1270..1722
                     /locus_tag="PKV64_09150"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HPN17281.1"
                     /translation="MDYLEFSARDAISRWDSCRAYRRATSNTAAAFLEYMEKKFPFFI
                     RAIQIDGGSEFMKNFEEACRQRRIRLFVIPPRSPKLQGCVERSNRTYREEFYEVEDIE
                     NQLEGHNRQLEEWNKTYNYIRPHQSLGYLTPYEFYQNWLMEHNKAKCH"
     gene            1813..2763
                     /locus_tag="PKV64_09155"
     CDS             1813..2763
                     /locus_tag="PKV64_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPN17282.1"
                     /translation="MEQITHPFVRKSVFIGVLDRNLKKIESRLGVELNIRGDSLNIGG
                     PPDKVEFARFYFDRIRQLEEKGHDLKEEDFQIILDLLEQGRTKPVEDYYPAEPLSFAR
                     KSVSPKSLNQQEYIQSIRTHDLVFGVGPAGTGKTYLAMAMALTFLQNKVVSRIILTRP
                     AVEAGERLGFLPGDMIQKVNPYLRPLYDALFDLVSVDQAQRLIDKNIIEIAPLAYMRG
                     RTLNAAFVILDEAQNTTRPQMKMFLTRAGFDSKVVVTADITQIDLPQPRDSGVLHALK
                     VLAPLKEIAVCRFNEKDVVRHPLVQKIIKAYQKAESRSSD"
     gene            2765..5149
                     /locus_tag="PKV64_09160"
     CDS             2765..5149
                     /locus_tag="PKV64_09160"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPN17283.1"
                     /translation="MPLVSIKDLKLFKAPGSPASGRPPEPERATGMKRIVRDPFLLIF
                     LSALVLATILSYLPSKQLSELEPGDIAGEDLIAPADLTIEDTETTEQRRREAVEAVLP
                     VYTLDPNVMLNAGDHIHQFFVYGREWLAAPPPRDYPALRSVILEKFGLELSIGDLQAL
                     AREGFGPDLEASLLTLLENVSAGGLILSKKLFVNREEERGLTLRLPDGSEKTVKAAEI
                     LEIKEAKNRLLEDVNALELSARKKGLLLSLSFGFLAPNMAFDRIETEIRKTNAWSTVE
                     TVFFTVKKGRVIIRKGDEAGEAAVRLIAAVNRAIADRGAWLGSFTGAFFLFALLFVTL
                     WYYLRTLHALPTARKYNQMIMLTIFLSLLLYKLGLFLGATTAANASFALLSSAEAYRF
                     ILPFQFGAILFAFLTTNAVALIYVVLNSLLAGYLTGADFYLMLFGLIGGLAAIYGIKF
                     YGRAKRTNALRAGFFIVTPVNLFVVITLFLIQPRVVAAPVLAAETMMSVVGGLMSGAL
                     AFLFLPIFESLFRLITQSKLLELTSSESGILRQMALEAPGSYHHSLIVSTLAEKAAEK
                     VRLDALLVKAGALYHDIGKIKRPEYFIENRDRKHDAHRDLSPSISTLVIVNHVKEGLE
                     IARKERLPQAVRDIIEQHHGNSIVRYFYQKAKEKCGPDIQDVGEETYRYPGPPPQTRE
                     AALVMLADSVEAASRSLRSHKDEHLARVIRDIFDNYFQDGQLDDCAFSLREIRTIAQS
                     FLSTLQTIYQPRIEYPGFDFESKKKPPKNGEPPASPRTPHGGNHQPPVEEPNQD"
     gene            5103..5549
                     /gene="ybeY"
                     /locus_tag="PKV64_09165"
     CDS             5103..5549
                     /gene="ybeY"
                     /locus_tag="PKV64_09165"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPN17284.1"
                     /translation="MVEIINRQSKSRIRTDRYAALLERLLRRARLKNADVVLVFAGDA
                     FVRRLNRVFRRKDRTTDVLSFPIDGRGEGGRRHLGDIVISVPQARRQAAALGHSLDAE
                     LAFLAIHGFLHLCGYEHEAGHEEEEARVLRLLRREKFGPLPAGPGE"
     gene            5553..6812
                     /locus_tag="PKV64_09170"
     CDS             5553..6812
                     /locus_tag="PKV64_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPN17285.1"
                     /translation="MEISTITWAGLGLCVLAASALSLFHITLSGYSKVGLSGFLEERN
                     HPDRTRILDRYDDIKIAVEFWRMILIIALAVYAFLIFPAFRTRPLGLFLFSAVVYAVF
                     FDIVPRLLAAAGRDRCLSRFLPSYRLFLSLSAPVLAFVRWLSVREEQKEEEAGPEDRE
                     AGEEEIETFLDEAEEEGIIEKGEDALLRNVVEFGDTIVREVMTPRVDMVCIRRDATIQ
                     KLRHLFIVEKYSRIPVYRDRIDGVDGLVMAKDLLAYSEKEFDEASIEPLVRPLLFVPE
                     SMKVADLLKELQKAKQKLAVVVDEHGGVSGLVTMEDVFEVIVGEIQDEYDAEEAQIVQ
                     NGPADYTVSGAAKVEEIEDLFDVELADDDFITVSGLVAQALGRLPTKGERLIIGRLIV
                     DVLDVDQKRIKKLRMRKAADEEPEHAG"
     gene            6802..7968
                     /gene="era"
                     /locus_tag="PKV64_09175"
     CDS             6802..7968
                     /gene="era"
                     /locus_tag="PKV64_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPN17286.1"
                     /translation="MPGKTSRAAPRTGKSRSAPKKPVSKFRTGYVALIGRPNAGKSTL
                     LNKILEQKIAITSDKPQTTRLSILGIHTTPRGQIIFIDNPGLHKPLHKLNKRMMNFVY
                     ASIETADIVCLLVDATAKFGHGDEFALEIIKNVRKPVFLLLNKTDIVRKEILLPLIDR
                     YRALREFQEFIPLSALKGTNVDVLENLLFDNLPRAEKLYGDDDVSDQSERFLLAEIIR
                     EKILARVEAELPWTTAVYIDRIDRKRGQEEEEPEDESGPEEAGGEEAPGEREALEEPE
                     PERETGRTIAVPGVSDRPNIRRRGEKPKPTREERRARPAVYIKASIFVERPNHRKIIV
                     GRRGQNIKEIGIEARREIEEILEARIFLDLQVKVRPQWRDAADVLDLIEGQKEA"
     gene            7993..8493
                     /locus_tag="PKV64_09180"
     CDS             7993..8493
                     /locus_tag="PKV64_09180"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPN17287.1"
                     /translation="MPTVLIVILSAAGLAAVSQALNVLSYRVLKARILRRRKWDLNIC
                     CGRTDGGGLNADIVRHADVPRFVQIGSIDRLPFKDGSFETVLCSHTMEHVENPDAFFE
                     ELRRVGNRVTLVLPPLWDLAGALNVLEHRWIFLTMRKEHHRLPSRIRLPLSRIVQRRL
                     GQRIHA"
     gene            8500..9378
                     /locus_tag="PKV64_09185"
     CDS             8500..9378
                     /locus_tag="PKV64_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17288.1"
                     /translation="MSPSATAMIYGIVGSILFNLSKGMQRHGIEALSCLSPKRAARRA
                     GRRRPGASKKAALYATGFLLNNSLGFFAILANRYGPSSYYTSMFGAGLIALMLYAGII
                     LKEPLRPLQYGGALVLTLGTLLLGYDGILRPEMTMAGVRLPAAGIILAASALGGLLIL
                     FRTRRKGGLTSLGIVCGLLIGFAAGLDPILKGIGQNLGGVDGRYLPRLPLGWVIFLSS
                     FLFATLSFAASQWAFRRGIRASVLIPSQNFSYIVYPVLFQAAALPGFRITGLTALGLT
                     VTVSGLLVMQASLRRT"
     gene            complement(9403..10608)
                     /locus_tag="PKV64_09190"
     CDS             complement(9403..10608)
                     /locus_tag="PKV64_09190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPN17289.1"
                     /translation="MLCKEKKLHRVKNRRPEREGGSSFPGGLRVALYAGMFVRDFDGA
                     TKTLFELIRSLQGRKAEIAVWTFSRRPTQLPGIRVFRVPAIALPFYPDYKFSLPWPLL
                     FFQIGRFRPDVIHLTVPDAIGLVLAVYAKLRRIPLVMSYHTDFVSYLEERNLWFLVWI
                     WWPILRVYYNLADTLFVPSRDSGAKLARHGIRATALWRRGLPEGLFSPAQRSESLRRR
                     WGAENKKVILFSGRFAWFKGLQVYVQVYDLFKKDGNFSPAFVLLGRGPLEEELKRRMP
                     EAVFPGYLNGDALSAVYASADLLLFPSTTETFGNVIQEAIASGLPAVVSDEGGCREIV
                     EETGGGLVARARRGPSFYRECRRLLTDESLYRRVRETGLEKIRGRNWEAVNRVVLDSY
                     ARLVCLHPR"
     gene            10788..10864
                     /locus_tag="PKV64_09195"
     tRNA            10788..10864
                     /locus_tag="PKV64_09195"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10822..10824,aa:His,seq:gtg)
BASE COUNT         2190 a         3497 c         3106 g         2071 t
ORIGIN      
        1 tcctgcgcta aattatccta tttcggaggg aaagagggga tttcatgacg agaccgggaa
       61 tatcgtcggg ggcttctgat aaaaggaaat tcctatcatt tcatcgacga aacacatcaa
      121 gaccaggaat tccttggcat gctttttgca cctcaaaaaa tgaaaggagg gcttaataaa
      181 tgagacctat tagatcaatg gtgttttttg gattatcagt attgttttac agccattatg
      241 cattgccaga ggaaaacaac aggctatcct ggcgtttctc ggcaggcaca tcctatttcg
      301 cttccaatat cgtgcggccg cttgaagagg catatatcga accggcaatt agcaacagcc
      361 ataagtcgtt aaacggccct tctccggcaa tgcgcctgga ggccagctat gacataaccc
      421 gcaacatgtt cgtgaatctg ggcgttgaat atatttttcc aagaacattc aaacagcgta
      481 tcgacttgcc caatgaattt gatgaagacg ttttacaaaa tgataagctc tcaatgattc
      541 ccgtgtccct ggggttgggc tattcgatcc tttcgactgc cagattgaac gtttccgtca
      601 tcgccggcgc cgacctcaac atcatattgg actcgttcgg tcggagaata tcgggtcgct
      661 tacaaaccgg tgcgtctgct ttcggaaacc tgcatcagac aaacttcggc ttccgggcag
      721 gcacaagaat cgcatttgac aatttctttc tcgatatctc tgcgcggtta tttgaaatca
      781 cccttcaagg tgatgttttc atgcagagaa catttctctt gccggatcaa acaacatacc
      841 gagtcgttaa tgaaaatttt aaagctggag atttttgggg acttgacaac aatccgatga
      901 cagtcatttc catcggcgtc ggattcaaaa tctaatgatt ttttccgctc tgtcggaatc
      961 gtcaattcca aagcctgtcg ctcgaaccgt cgcatttcgc cggccgggat tcggcaaggt
     1021 taattgatat tcatcctcat ctctccaaag cggcacgagt tcatattagg aagctcgcca
     1081 tcgaacttgg cgatgccttc gtccgagagg gcgatggggt gcgcggagtt atcgttcgtt
     1141 gacaggccaa gaggtcggtt tcggcatctt ctctgataag cttccggtac gttgtttgaa
     1201 caaagaggtc ctctaacttc tcgtcgataa ggggccgctc agcgcggagc ggacggggaa
     1261 caggagtcga tggattatct ggagttcagc gcccgggacg ccatctcccg gtgggatagc
     1321 tgccgggctt atcgaagagc gaccagcaac acggctgcgg ccttcctgga atacatggaa
     1381 aagaagtttc cgtttttcat ccgggcgatc caaatcgacg gcggatccga gtttatgaaa
     1441 aacttcgagg aggcgtgccg acagcgccga atccggctgt tcgtgattcc tccgaggtcg
     1501 cccaagctgc aggggtgcgt cgagcgatcc aaccggacat atcgagaaga attttatgag
     1561 gtggaagata tcgaaaatca gttggaaggc cataatcgtc aactggagga atggaataag
     1621 acttataact atatccgccc ccatcagtct cttggatatc taacccccta tgagttttat
     1681 caaaactggt taatggagca taataaagct aagtgtcact aatctactgg acgagttcac
     1741 gtccttgaca agggggacgg agggctggat aatggattcc cgtggacgac tgcagcttta
     1801 cgcgcccttg atatggaaca aatcacccat ccgtttgtgc ggaaaagtgt gtttatcggc
     1861 gttctcgacc gcaatctgaa aaaaatcgaa tcccgcctgg gcgtcgagct gaacatccgg
     1921 ggggattccc tcaacatcgg cggaccgccc gacaaggtcg aattcgcccg tttctatttc
     1981 gaccggattc gccagctcga ggagaaggga cacgacctca aggaggaaga cttccagatc
     2041 atcctcgatc tgctggaaca ggggcggacc aagccggtgg aggactatta cccggccgag
     2101 cccttgagct tcgcccgcaa atccgtctcc cccaagagtc tcaaccagca ggaatacatt
     2161 caatccatcc ggacccatga cctggtcttc ggcgtcggcc cggccggaac gggaaaaacc
     2221 tatttagcca tggccatggc cctgacgttc ctgcagaaca aggtcgtcag ccggatcatc
     2281 ctgacccggc cggccgtcga ggccggcgag cgcctgggct tcctgcccgg cgacatgatc
     2341 caaaaagtca atccctatct tcggccgctc tacgacgcgc tgttcgacct ggtttcggtc
     2401 gaccaggccc agcggctgat cgacaagaac atcatcgaga tcgcccccct ggcctacatg
     2461 cgcggccgga ctttgaacgc ggcgtttgtc atcctcgacg aagcccagaa caccacccgg
     2521 ccgcagatga agatgttcct gacccgggcc ggattcgatt ccaaggtcgt cgtgaccgcc
     2581 gacatcaccc aaatcgatct cccccaaccc agggattcgg gcgtccttca cgccctcaag
     2641 gtcctcgcgc ctctcaagga gatcgccgtc tgccggttca acgaaaagga cgtcgtccgg
     2701 catcccctcg tccagaaaat catcaaggcc taccaaaagg ccgagtcccg ttcctccgac
     2761 tgacatgccc ctcgtctcca tcaaggatct caagctgttc aaggcgccgg gttccccggc
     2821 ctccgggcgc ccgcccgagc cggagagggc gacgggcatg aagcggatcg tccgggatcc
     2881 attccttctc attttcctgt ccgccctggt cctggccacg atcctttcct accttccgtc
     2941 caaacagctc tccgagctcg aacccggcga catcgccggc gaggacctta tcgccccggc
     3001 cgacctgacg atcgaggaca cggagacgac cgagcagcgc cgccgggagg ccgtcgaggc
     3061 ggttcttccc gtctacacgc tggatcccaa cgtgatgctg aacgcggggg accacatcca
     3121 tcaatttttc gtctacggcc gggaatggct cgccgcgccg ccgccccggg attatcccgc
     3181 cctacggagc gtcatccttg aaaagttcgg ccttgagctt tccatcggcg acctccaggc
     3241 cctggcccgg gaaggattcg gcccggacct ggaagccagc ctcctgaccc tcctggaaaa
     3301 tgtgtccgcc ggcggcctga ttctctcgaa aaagctcttt gtcaaccggg aggaggagcg
     3361 cggcttgacc ctgaggcttc cggacgggtc ggaaaagacg gtcaaggccg ccgagatcct
     3421 cgaaatcaag gaagccaaga accggcttct cgaggacgtc aacgccctcg agctttcggc
     3481 ccggaaaaaa ggcctcctcc tcagcttgtc gttcggcttc ctcgccccga acatggcctt
     3541 cgaccgcatc gaaacggaaa tccggaaaac caacgcttgg tccacggtcg aaaccgtgtt
     3601 tttcaccgtc aagaaggggc gggtcatcat ccggaagggc gacgaagccg gcgaggccgc
     3661 cgtccggctg atcgcggccg tcaaccgggc gatcgccgac cggggagcct ggctcggctc
     3721 tttcaccggc gccttttttc tcttcgccct cctgttcgtg acgctctggt actatctccg
     3781 gacgctccac gcccttccga ccgcccggaa atacaaccag atgatcatgc tgacgatttt
     3841 tttgagcctc ctcctgtaca agctggggct tttcctgggc gcgacgacgg ccgccaatgc
     3901 aagcttcgcc cttctttcca gcgccgaggc gtaccggttc atccttccgt tccagttcgg
     3961 ggcgattctc ttcgcctttc tcacgaccaa tgccgtcgcc ttaatctatg tcgttctcaa
     4021 cagcctgctc gccggatacc tgaccggggc cgatttctac ctcatgctct tcggcctgat
     4081 cggcgggctg gccgcgatct acgggatcaa gttctacggc cgggccaagc ggaccaacgc
     4141 tctccgggcg ggcttcttca tcgtcacgcc cgtcaatctt ttcgtcgtga tcaccctttt
     4201 tctcatccaa ccccgcgtcg tcgcggcccc cgtcctggcc gccgagacga tgatgtccgt
     4261 cgtgggcgga ctgatgagcg gcgccctggc ttttctcttc ctgccgatct tcgagagtct
     4321 tttccggctc atcacccagt cgaagctcct ggaattgacc agctccgaat cggggattct
     4381 ccgccaaatg gccctcgagg ctccaggctc ctatcaccat tccctcatcg tctccaccct
     4441 ggccgagaaa gccgctgaaa aggttaggct cgacgccctc ctggtcaagg ccggggccct
     4501 gtaccacgac atcgggaaaa tcaagcggcc cgagtatttc atcgaaaacc gggaccgcaa
     4561 acacgacgcc caccgggatc tgtccccgag catcagcacg ctggtcatcg tcaatcacgt
     4621 gaaagaggga ctggaaatcg cccggaagga gcgcctgccg caggcggtcc gggacatcat
     4681 cgaacagcac cacggcaatt cgatcgtccg ctacttctac cagaaagcca aggaaaaatg
     4741 cggacccgac atccaggatg tcggcgagga aacctaccgg tacccgggac cgccgccgca
     4801 aacccgggaa gccgccctgg tgatgctcgc cgattccgtc gaggccgcct cgcgcagcct
     4861 ccggtcccac aaggacgagc acctggcccg ggtcatccgc gacattttcg ataattattt
     4921 ccaggacggc cagttggacg actgcgcctt ttccctcagg gaaatccgaa cgatcgccca
     4981 gtcgttcctc tcgaccctcc aaaccatcta ccagccgcgg atcgaatatc cgggattcga
     5041 cttcgaatcc aagaaaaaac cccccaagaa cggcgaaccg ccggcttccc cgaggacccc
     5101 ccatggtgga aatcatcaac cgccagtcga agagccgaat caggactgac cggtacgcgg
     5161 ccttgctcga gcggctgctt cgccgggccc ggctgaaaaa cgccgatgtc gtcctcgtct
     5221 tcgccggcga cgctttcgtc cgccgcctga accgggtatt ccgccggaag gaccggacga
     5281 ccgacgtcct gtccttcccc atcgacggcc ggggcgaggg aggacggcgc cacctgggcg
     5341 atatcgtcat ctccgttccc caggcccggc ggcaagcggc cgccctcggt cacagcctag
     5401 acgccgagct cgccttcctc gcgatccacg gctttcttca tctctgcggg tacgagcatg
     5461 aagccggcca cgaggaggag gaagcccggg tgctccggct tctccggcgg gaaaaattcg
     5521 ggcctctgcc cgcggggccc ggggaatagg cgatggagat ttcgacgatc acctgggccg
     5581 ggctggggct gtgcgtcctg gccgcctcgg ccctgtcgct gttccacatc acgctctccg
     5641 gttattccaa ggtcggcctg agcggcttcc tggaagagcg gaaccacccg gaccggacgc
     5701 ggatcctcga ccgctacgac gacatcaaga tcgccgtcga attctggcgg atgatcctga
     5761 tcatcgccct ggccgtatac gcctttttga tcttcccggc cttccggacc cggccgctgg
     5821 ggctctttct cttttccgcc gtcgtttacg ccgttttctt cgacatcgtt ccccgccttc
     5881 tggccgccgc cgggcgggac cgctgcctct cccgcttcct cccgtcctac cgcctctttc
     5941 tttccttgag cgcgcccgtc ctggccttcg tccgctggct ctcagtccgc gaggagcaga
     6001 aggaggaaga ggccgggccc gaggaccggg aggccgggga ggaggaaatc gagacgttcc
     6061 tcgacgaggc cgaggaagaa ggcatcatcg agaagggcga agacgcgctg ctgcggaacg
     6121 tggtcgagtt cggagacacg atcgtccgcg aggtcatgac cccgcgggtg gacatggtct
     6181 gcatccgccg cgacgcgacc atccagaagc tccgccatct tttcatcgtc gagaaatatt
     6241 cccgcatccc cgtctaccgc gaccggatcg acggcgtgga tggattggtc atggccaagg
     6301 atctcctggc ctattcggaa aaggagttcg acgaggcctc gatcgagccc ctggtccggc
     6361 cgctcctctt cgtgcccgaa tccatgaaag tggccgacct cctcaaggaa ctccaaaaag
     6421 ccaagcagaa gctggccgtg gtggtcgacg agcacggcgg cgtctccggc ctggtgacga
     6481 tggaggacgt cttcgaggtg atcgtcggcg agatccagga cgaatacgac gccgaggagg
     6541 cccagatcgt ccagaacggc ccggccgatt acaccgtctc cggggcggcc aaggtcgagg
     6601 aaatcgagga tcttttcgac gtggagctgg ccgacgacga cttcatcacc gtcagcgggc
     6661 tggtggccca ggcgctcggc cgcctgccga ccaaggggga gcgcctgatc atcggccggt
     6721 tgatcgtcga cgtcctggac gtcgaccaga agcggatcaa gaagctgagg atgaggaaag
     6781 ccgccgacga ggagcccgaa catgccgggt aagacgtccc gcgccgctcc ccggacgggc
     6841 aaatcccgct ccgccccgaa gaagccggtt tcgaaattcc ggacgggcta cgtcgccctg
     6901 atcggacggc ctaacgccgg caaatccacc cttctcaaca agatcctgga gcagaagatc
     6961 gccatcacct cggacaagcc gcagacgacc cgcctgagca tcctgggcat ccacacgacg
     7021 ccgcggggcc agatcatctt catcgacaac cccggcctcc acaagccgct ccacaagctg
     7081 aacaagcgga tgatgaactt cgtctacgcc tccatcgaga cggccgacat cgtctgcctc
     7141 ctcgtcgacg ccacggcgaa attcggccac ggcgacgaat tcgccctgga gatcatcaag
     7201 aacgtccgga agccggtgtt tctcctgctg aacaagacgg acatcgtccg aaaggagatc
     7261 ctcctcccgc tcatcgaccg gtatcgggcc ctccgggaat tccaggaatt catccccctg
     7321 tcggccttga aggggaccaa cgtcgacgtc ctggaaaatc ttctcttcga caacctcccc
     7381 cgggcggaga agctctacgg cgacgacgac gtctccgacc agtccgagcg ctttctcctg
     7441 gccgagatca tccgggagaa aatcctggcc cgggtggaag ccgagcttcc ctggaccacg
     7501 gcggtctaca tcgaccggat cgaccggaag cgggggcaag aggaggagga gccggaagac
     7561 gaaagcgggc cggaagaagc cggcggggag gaagcgcccg gtgaaaggga agccctcgag
     7621 gagcccgagc ccgagcggga aaccggccgg acgatcgccg tcccgggcgt ctcggaccgg
     7681 ccgaacatcc ggcggcgcgg ggaaaagccg aaaccgaccc gggaagaacg ccgggcccgg
     7741 cccgccgtct acatcaaggc gagcatcttc gtcgagcgcc ccaatcaccg gaaaatcatc
     7801 gtcggccgcc gggggcagaa catcaaggaa atcgggatcg aagcccggag ggagatcgag
     7861 gagatcctcg aagcccggat cttcctcgac ctgcaagtca aagtccgtcc ccagtggcgg
     7921 gacgcggccg acgtcctcga cctcatcgag ggccagaagg aagcctagcc ggagaacggc
     7981 ggagcgccgc ggatgccgac cgtcctgatc gtgatcttaa gcgcagccgg cttggccgcc
     8041 gtctcccaag cgctcaacgt cctgagctat cgcgtcctca aggcgcgcat tctccgccgg
     8101 cggaaatggg atctcaacat ttgctgcggc cggacggacg gcgggggtct caacgccgac
     8161 atcgtccgtc acgcggacgt gccgcgtttc gtccagatcg gctcgatcga ccgtcttccg
     8221 ttcaaggatg gctcctttga gaccgtcctc tgctcgcaca cgatggagca tgtcgagaat
     8281 ccggacgcct ttttcgagga gctccggcgc gtcggaaacc gggtgaccct cgttctcccg
     8341 cccctctggg acctggccgg agccctgaac gtcctggagc accgctggat cttcctgacg
     8401 atgcgcaagg agcatcaccg gctgccgtcc cggatccgcc tccccttgtc ccggatcgtc
     8461 cagcggcggc tggggcagcg catccacgcc tagccgccca tgtctccttc cgccacggcc
     8521 atgatctatg ggatcgtcgg atcgatcctg ttcaacctct ccaagggaat gcagcgccac
     8581 ggcatcgaag ccctctcctg cctttcccca aaacgggccg cccggcgggc cggccgccgc
     8641 cggccgggcg catcgaaaaa ggcggccctc tacgcgacgg ggttcctcct caacaacagc
     8701 ctgggctttt tcgccatcct ggccaaccgc tacggcccgt cgtcctatta cacctccatg
     8761 ttcggcgccg gactcatcgc cttgatgctc tacgccggga tcatcctcaa ggagcccctc
     8821 cgcccgctcc agtacggcgg ggcgctggtt ctcaccctgg gaaccctcct gctcggttat
     8881 gacggcatcc tccgtccgga aatgaccatg gccggcgtcc gtcttccggc cgcgggaatc
     8941 atcctcgccg cctcggccct ggggggcctg ctgatcttat tccggacgcg gcggaagggc
     9001 gggttgacga gcctggggat cgtctgcgga ctgctcatcg gcttcgccgc cggtctcgac
     9061 cctatcttga aagggatcgg ccagaatctg ggaggagtcg acgggcgcta tcttccccgg
     9121 ctgccgctcg gatgggtgat ctttctctcc agcttcctct tcgccaccct gtcttttgcc
     9181 gcgtcccaat gggcgttccg aagggggatc cgcgcttccg tcctcatccc ttcccaaaat
     9241 ttttcctata tcgtctaccc ggtgcttttc caggccgccg ccctccccgg cttcaggatc
     9301 acggggctca ccgccttggg gctgaccgtc accgtcagcg gcctcctcgt catgcaagcg
     9361 tctctccggc ggacctagcc tccgggaacc gcggacctcc gctcagcgcg gatggagaca
     9421 aaccaggcgg gcgtaagaat ccaagaccac tcggttgacg gcttcccagt ttcttccgcg
     9481 gatcttttcc aagcccgtct cccggacccg gcgatacagc gattcgtcgg tcagcaggcg
     9541 gcggcactcc cggtaaaacg acgggccccg ccgggcccgg gccaccaggc cgccgcccgt
     9601 ttcctcgacg atctcccggc atccgccctc gtccgagacg accgccggca gacccgaggc
     9661 gatggcttcc tggatcacgt tgccgaacgt ctccgtcgtc gaggggaaca gaagaagatc
     9721 ggcgctggca tagacggccg agagcgcgtc tccattgaga tagcccggga agacggcttc
     9781 cggcatccgc cgtttcagct cctcctccag cggaccacgg ccgagaagga cgaaggccgg
     9841 cgaaaagttc ccgtcttttt taaagaggtc gtaaacctgg acgtacacct ggagaccctt
     9901 gaaccaggcg aaccggccgc tgaaaagaat gacttttttg ttttcggccc cccagcggcg
     9961 gcgcaaggat tcgctccgct gagcggggct gaagaggccc tcgggcaatc cccgccgcca
    10021 cagggccgtc gcccggatgc cgtgccgggc cagcttcgcc ccgctgtccc ggctggggac
    10081 gaagagcgtg tcggcgaggt tgtaatacac gcgcagaatc ggccaccaga tccagaccag
    10141 gaaccagagg ttgcgctcct ccaggtagga gacgaaatcg gtgtgatacg acatgacgag
    10201 ggggatcctt cggagcttgg cataaacggc caggacgagg ccgatggcgt cgggtacggt
    10261 caaatggatc acgtcgggcc ggaatctccc gatttgaaaa aacaggagcg gccagggaag
    10321 ggaaaactta taatccggat aaaacgggag ggcgatggcc ggaacccgga agacgcggat
    10381 gcccggaagc tgggtcgggc ggcgggaaaa cgtccagacg gcgatctcgg ccttacgccc
    10441 ctgaagggac cggatcagct cgaacagggt tttcgtcgcc ccgtcgaaat cccggacgaa
    10501 catgccggcg tagagggcga cgcgcaaacc tccggggaaa gaagagcccc cctcgcgttc
    10561 cggacggcgg tttttcaccc ggtgaagctt tttttcttta cataacatct attctttgct
    10621 actggccata gaataccata aaccggggga agcgaaaaaa cactttgaaa aagtcatccg
    10681 ccgatagcct aaacgtccgg ccgagccgga atgatgcagc cgtcgtcgcc gggaagttga
    10741 cttctccggc gccaaatgcg aaaattcgga ctcctcacgt tcggacggtg ggtgtagctc
    10801 aaccggttag agcatcggac tgtggatccg aaggttgggg gttcgagtct cctcacccac
    10861 ccca
//
LOCUS       DAOOXH010000084        10612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_6715_length_10612_cov_7.874017, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000084 DAOOXH010000000
VERSION     DAOOXH010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 10612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10612
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6715_length_10612_cov_7.874017"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..517)
                     /locus_tag="PKV64_09200"
                     /pseudo
     CDS             complement(236..517)
                     /locus_tag="PKV64_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(427..1722)
                     /locus_tag="PKV64_09205"
     CDS             complement(427..1722)
                     /locus_tag="PKV64_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17290.1"
                     /translation="MPKDKAHPLAYLSESGAFLISRISRRPWLEPLYAISLGSQADLR
                     LSDYLRFLRDETEVKVVAVYVESFMAGDGYLAAKAVREITRSPGRSVIIYKGGRSPAK
                     EGSGAEELDGIGVERGKKWTRGEGSAEGTEERERSERGEWAAEKSAGMSVRAGAIAGA
                     SAGMEKRMDGGMDKGEEKKPEKKTEARPGADYELFRAVFEDAGALIADTIAEFESFVS
                     GALLLGGKRVGGNRVALMSNAGFECVVMSDNLGNGEERLEPAAFSTETMRRIGEALAP
                     LGINKIQDIRNPLDVTPVADDAAFAAAAGAVLDDPGVDCAVISPLPLGPVLQTLPAGE
                     GHREDMEGEGSIGKRLIEIFKATDKPFVVNVDSGADYDPLCAMLEEAGIPVFRDCDAA
                     VRFMRKWVGRRRIWTTPSRSMISRTSTRNGSPRPTARSG"
     gene            complement(1735..4128)
                     /locus_tag="PKV64_09210"
     CDS             complement(1735..4128)
                     /locus_tag="PKV64_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPN17291.1"
                     /translation="MTTDFKKIDAIFAKAEADGRRFLLEPEGYAMLKLAGVAVPRHKF
                     VPAGKKLSAADVAALKSPAGLVLKIVAPLIQHKTDVGGVAFVKASAPAANAAIKKMIA
                     EVPGRFLAWSKAHSDSAARGAAPAAAASSSSPAGLTAKDVAADIRGVLVCEQVAFEKF
                     GFGSELLLGARNSREFGPVVTFGAGGVEVEYLGERLKEGTAAAMASAHLLAPKKILDH
                     LSAAAVYDKLVKPFRGKPAPLKPVVLRETYARFQALTAHYSPFGPASRVVIEEIEVNP
                     FVVRAGRLVPLDLLCRFSRDHVDVKNRPVAGIRPLLRPASIGVIGVSEKMNLGHVILN
                     NVIEMGFPKDRVYVVKPGLAEIEGCRCVPTVADLPETVDMFVLTLSAEQCEPVMRDLI
                     AHDKARSVIIIAGGMGEKSGTQSIEKKIVDLLAESRKAGRPAPAVNGGNCLGIYSRPG
                     SYDTTFIPRYKLRFPKTGIPGLVYVSQSGAFMISRISRLQRFVPLYGISIGNQIDLRV
                     SDYLRYLKDEPEAKVVALYIEGFKPGDAYPTAAAAREITRVPGRYVIAYKSGRSPEGR
                     LATSSHTASVAGDYGICKAVLEEAGIIVADTIAEFESYLSGALMLGGKRWAGNRVVMM
                     STAGFESVIMSDNLRNGTGSGGTLELAVYTDETKRKLEEALASLGIDKFQDVRNPLDT
                     TPVADDTVFSACAQAVLEDPGVDCAVISVHPLTGSLQTIAPAEGHPQNVYDPSSVGPR
                     LIEIFKATDKPFVVSIDAGVLYDPLADLLQEAGIPVFRDCDAAVKFLRKFAGAATKR"
     gene            complement(4291..5262)
                     /locus_tag="PKV64_09215"
     CDS             complement(4291..5262)
                     /locus_tag="PKV64_09215"
                     /inference="COORDINATES: protein motif:HMM:NF016138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HPN17292.1"
                     /translation="MLAGTVSERLVLPARISMELVDFVVPVACAGLNSGIGSGTLDIP
                     KAKANPFNARVSANVTGVMKAAGVIRDGFGFQVGSGAGLVVLDDIRTILKERKIQAHF
                     TIGGITSLHVDMLEEGTVRHLMNGQMFEPSPRVIASMLENPNHHEVSTGYYASTANKE
                     VAVNMLDAAVLSALEVDLGFNVNTVCAGGRIIGGIGGGQDVAAGSALTIIFLPLATGK
                     NGKGFPKVVQKVYTRTTPGEVIDVVVTEEFVAVNPAGASIYKDALLANGAAVGLKMIS
                     IEELHAKSVEAAKAFGTIPSAPQATGDIVHAIEWRDGTLLDVIRKTE"
     gene            5351..6322
                     /locus_tag="PKV64_09220"
     CDS             5351..6322
                     /locus_tag="PKV64_09220"
                     /inference="COORDINATES: protein motif:HMM:NF016138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HPN17293.1"
                     /translation="MLAGTVSERLVLPARISMELVDFVVPVACAGLNSGIGSGTLDIP
                     KAKANPFNARVSANVTGVMKAAGVIRDGFGFQVGSGAGLVVLDDIRTILKERKIQAHF
                     TIGGITSLHVDMLEEGTVRHLMNGQMFEPSPRVIASMLENPNHHEVSTGYYASTANKE
                     VAVNMLDAAVLSALEVDLGFNVNTVCAGGRIIGGIGGGQDVAAGSALTIIFLPLATGK
                     NGKGFPKVVQKVYTRTTPGEVIDVVVTEEFVAVNPAGASIYKDALLANGAAVGLKMIS
                     IEELHAKSVEAAKAFGTIPSAPQATGDIVHAIEWRDGTLLDVIRKTE"
     gene            6485..8878
                     /locus_tag="PKV64_09225"
     CDS             6485..8878
                     /locus_tag="PKV64_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPN17294.1"
                     /translation="MTTDFKKIDAIFAKAEADGRRFLLEPEGYAMLKLAGVAVPRHKF
                     VPAGKKLSAADVAALKSPAGLVLKIVAPLIQHKTDVGGVAFVKASAPAANAAIKKMIA
                     EVPGRFLAWSKAHSDSAARGAAPAAAASSSSPAGLTAKDVAADIRGVLVCEQVAFEKF
                     GFGSELLLGARNSREFGPVVTFGAGGVEVEYLGERLKEGTAAAMASAHLLAPKKILDH
                     LSAAAVYDKLVKPFRGKPAPLKPVVLRETYARFQALTAHYSPFGPASRVVIEEIEVNP
                     FVVRAGRLVPLDLLCRFSRDHVDVKNRPVAGIRPLLRPASIGVIGVSEKMNLGHVILN
                     NVIEMGFPKDRVYVVKPGLAEIEGCRCVPTVADLPETVDMFVLTLSAEQCEPVMRDLI
                     AHDKARSVIIIAGGMGEKSGTQSIEKKIVDLLAESRKAGRPAPAVNGGNCLGIYSRPG
                     SYDTTFIPRYKLRFPKTGIPGLVYVSQSGAFMISRISRLQRFVPLYGISIGNQIDLRV
                     SDYLRYLKDEPEAKVVALYIEGFKPGDAYPTAAAAREITRVPGRYVIAYKSGRSPEGR
                     LATSSHTASVAGDYGICKAVLEEAGIIVADTIAEFESYLSGALMLGGKRWAGNRVVMM
                     STAGFESVIMSDNLRNGTGSGGTLELAVYTDETKRKLEEALASLGIDKFQDVRNPLDT
                     TPVADDTVFSACAQAVLEDPGVDCAVISVHPLTGSLQTIAPAEGHPQNVYDPSSVGPR
                     LIEIFKATDKPFVVSIDAGVLYDPLADLLQEAGIPVFRDCDAAVKFLRKFAGAATKR"
     gene            8891..10186
                     /locus_tag="PKV64_09230"
     CDS             8891..10186
                     /locus_tag="PKV64_09230"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17295.1"
                     /translation="MPKDKAHPLAYLSESGAFLISRISRRPWLEPLYAISLGSQADLR
                     LSDYLRFLRDETEVKVVAVYVESFMAGDGYLAAKAVREITRSPGRSVIIYKGGRSPAK
                     EGSGAEELDGIGVERGKKWTRGEGSAEGTEERERSERGEWAAEKSAGMSVRAGAIAGA
                     SAGMEKRMDGGMDKGEEKKPEKKTEARPGADYELFRAVFEDAGALIADTIAEFESFVS
                     GALLLGGKRVGGNRVALMSNAGFECVVMSDNLGNGEERLEPAAFSTETMRRIGEALAP
                     LGINKIQDIRNPLDVTPVADDAAFAAAAGAVLDDPGVDCAVISPLPLGPVLQTLPAGE
                     GHREDMEGEGSIGKRLIEIFKATDKPFVVNVDSGADYDPLCAMLEEAGIPVFRDCDAA
                     VRFMRKWVGRRRIWTTPSRSMISRTSTRNGSPRPTARSG"
     gene            10096..10377
                     /locus_tag="PKV64_09235"
                     /pseudo
     CDS             10096..10377
                     /locus_tag="PKV64_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1926 a         3380 c         3380 g         1926 t
ORIGIN      
        1 cgaaccccgc acatcatatt gattttttcc ccgtcaatca atgaaaaaaa taggtatttg
       61 ctttttcgcc ctcggctctc atccagaacc ggcatgagac ccagtcattc ccccaccgag
      121 ggagctaaac cggacctcgg gttatgttaa gaacggccgg aagaaccgga aaacggtact
      181 ggacgctcag ccgtgtcaac ggatgtttgc gttttagggg ccatcgtaac tctcttcaac
      241 acccgatttg acttgtcagt tcatgaagct tttccaagga tttgatgatc ttgtcctctc
      301 gaatgtagct tcgctccccg ctccagtcct ccgtatattc gaccaggtag cagatcgtaa
      361 gtctgatata ggactcgacg gatggaaaaa cgccgacgac ccggcttcgc cggcggatct
      421 cgcgattcaa ccgctcctgg ccgttggccg aggagatccg tttcttgtcg atgtccggga
      481 aatcatagaa cgagagggag tcgtccaaat cctcctcctc cccacccact ttctcataaa
      541 cctcaccgcc gcgtcgcaat ccctgaacac cgggatcccg gcctcctcga gcatcgcgca
      601 cagcgggtcg taatccgccc ccgaatcgac attgacgaca aacggcttgt cggtcgcctt
      661 gaaaatctcg atcagccgct tcccgatgct cccctcgcct tccatatcct ccctgtgccc
      721 ctcccccgcc ggcagcgtct gaagcaccgg gcccaaggga agaggcgaga tgaccgcgca
      781 atccaccccc gggtcgtcga gcaccgcccc cgcggccgcg gcgaacgccg cgtcgtcggc
      841 caccggcgtc acgtccaagg gattccggat gtcctggatc ttgttgatcc ccagcggggc
      901 cagcgcctcc ccgatccgcc gcatcgtctc ggtggaaaaa gcggccggct ccagccgttc
      961 ctccccgttc ccgaggttgt ctgacatgac gacgcattcg aacccggcgt tgctcatcag
     1021 ggcgacgcgg ttcccgccga cgcgctttcc gcctaacagc aaagccccgg aaacgaagct
     1081 ctcgaactcg gcgatcgtat cggcgatgag ggcgcccgcg tcctcgaaaa cagcccggaa
     1141 aagctcgtaa tcagcccccg gcctcgcctc cgtttttttc tccggcttct tttcttctcc
     1201 cttatccatc cccccatcca tccgcttttc catccccgcg ctcgcgccgg ctatcgcacc
     1261 cgccctcacg ctcatccccg cgcttttttc cgctgcccat tctcctctct cgctccgctc
     1321 tctctcctcc gtcccttccg cgcttccctc cccccgcgtc cacttttttc ccctctccac
     1381 cccgattccg tccaattcct ccgcccctga tccttctttc gccggggaac ggccgccctt
     1441 ataaatgatc acgctccgtc cgggcgaccg ggtgatctcc cggaccgcct tggccgccag
     1501 atacccgtcg cccgccataa agctctcgac gtagaccgcg accaccttca cctcggtctc
     1561 gtctcttaga aaacgcagat aatccgagag gcgcaggtcc gcttggctcc ccagcgaaat
     1621 cgcgtacagc ggctcaagcc aaggccggcg ggagatccgg ctgatgagga aggccccgct
     1681 ttcgctgaga tacgccaacg gatgagcctt gtctttgggc atgcgccctc gtcctcaccg
     1741 cttcgtcgcc gctcccgcga acttcctcag gaacttgacc gccgcgtcgc aatcccggaa
     1801 caccgggatg ccggcctcct ggagcagatc ggccagcggg tcgtacaaga cgccggcgtc
     1861 gatgctgacg acgaacggct tgtccgtcgc cttgaaaatc tcgatcaacc tcgggccgac
     1921 gctcgaggga tcgtagacgt tctggggatg cccttcggcc ggagcgatcg tctgcaggga
     1981 cccggtcagc ggatgcaccg aaatgaccgc gcaatccacc ccgggatcct ccaagacggc
     2041 ctgggcgcag gcggagaaga ccgtgtcgtc ggccacgggc gtcgtgtcga gcggattgcg
     2101 gacgtcttgg aacttgtcga tcccgaggga ggccagggcc tcctccagct tccgtttcgt
     2161 ttcgtccgta tagacggcca gctcgagggt tccaccgctc cccgtcccgt tccggaggtt
     2221 gtccgacatg atgacgcttt cgaatccggc cgtgctcatc atgacgaccc ggtttccggc
     2281 ccagcgcttt ccgccgagca tcagcgcgcc ggaaaggtaa ctttcgaact cggcgatcgt
     2341 gtcggccacg atgatcccgg cttcctcaag cacggccttg cagatgccgt agtcgccggc
     2401 cacggaggct gtgtggctgg aggtcgccag acgcccctcc ggcgagcggc cgctcttgta
     2461 ggcaatgacg taccggccgg gaactcgggt gatttcccgg gccgccgcgg ccgtcgggta
     2521 cgcgtcgccc ggcttgaatc cctcgatata gagggcgacg accttcgctt caggctcatc
     2581 cttgaggtag cggagataat cagagacgcg caggtcgatc tggttgccga tggaaatgcc
     2641 gtagagcggg acgaagcgct ggaggcggga aatccggctg atcatgaacg ccccgctctg
     2701 gctgacgtag accaggccgg ggatcccggt tttgggaaag cggagcttgt agcgggggat
     2761 gaacgtcgtg tcgtaagagc ccggccggct gtagatcccg aggcagtttc cgccgttgac
     2821 cgcgggcgcg ggccggccgg ccttgcggct ctcggccaaa aggtcgacga tcttcttctc
     2881 gatggactgg gtcccggact tttcccccat cccgccggcg atgatgatca ccgagcgggc
     2941 cttgtcgtgg gcgatgagat cgcgcatgac cggctcgcac tgctcggcgc tcagggtgag
     3001 gacgaacatg tccaccgtct ccggcaggtc ggccacggtg gggacgcacc ggcaaccctc
     3061 gatctcggcg aggcccggtt tgaccacgta gacgcggtcc ttcggaaagc ccatctcgat
     3121 gacgttgttg aggatgacat gcccgagatt cattttctcc gagacgccga tgacgccgat
     3181 cgaggcgggc cgaagcagcg ggcggatccc ggcgacgggg cggtttttga cgtccacgtg
     3241 gtcgcgggag aagcggcaca gcagatcgag cggcacgagg cggccggccc ggacgacgaa
     3301 gggattgacc tcgatctcct cgatgacaac ccgcgaggcc ggcccgaacg gcgaataatg
     3361 cgcggtcagg gcctggaacc gggcgtaggt ctcccggagg acgaccggct tgaggggggc
     3421 cggcttgccg cggaagggct tgaccagctt gtcgtaaacg gccgccgcgg agaggtggtc
     3481 gaggattttt tttggggcga gaagatgggc cgaggccatg gccgcggccg tcccttcctt
     3541 gaggcgctcg ccgagatatt caacctcgac gcctccggct ccgaaggtca cgaccggccc
     3601 gaactcgcgg gaattgcggg ccccgagaag aagctccgag ccgaagccga acttctcgaa
     3661 agcgacctgc tcgcagacga gcacccctcg gatgtcggcc gcaacgtcct tagcggtgag
     3721 gccggcgggg gaagaggagg aagctgcggc ggcgggagcg gcccccctgg ccgcggaatc
     3781 ggaatgagcc ttggaccaag ccaggaagcg tccgggaact tcggcgatca tcttcttgat
     3841 ggccgcgttg gccgcggggg cggaggcctt gacgaaggcg acgccgccga cgtcggtttt
     3901 gtgctggatg agcggggcga cgatcttgag gacgaggccg gccggagatt tgagggcggc
     3961 gacgtcggcc gccgagagct tttttccagc ggggacgaac ttgtggcggg ggacggcgac
     4021 gccggccagc ttgagcatgg catagccttc gggctcgagc aggaagcggc ggccgtcggc
     4081 ctcggccttg gcgaagatcg cgtcgatttt cttaaagtcg gtggtcatga agactccctc
     4141 gatgagcggc gatacgagaa acttgaaata ggagacagga tcccaagacg gaaaagaaac
     4201 gggagccccg gtccccattc ataagaatgg ggaccgatct cccccgtttc gcgcgtcttc
     4261 tttatccgcg tctctcattt cccccctttt ctactcggtc ttgcgaatga cgtcaagcag
     4321 cgtgccgtcg cgccattcaa tggcgtgaac gatgtcgccg gtcgcctgcg gcgcggacgg
     4381 aatggtcccg aaagccttgg ccgcctcgac cgacttggcg tgcagctcct cgatcgagat
     4441 cattttcagc ccaaccgcgg ccccgtttgc caggagagca tctttataaa tggaggcgcc
     4501 ggccggattg accgccacga attcctcggt cacgacgacg tcgatcacct cgcccggcgt
     4561 cgtccgggtg tagacttttt ggacgacctt ggggaaaccc ttgccgttct tgccggtggc
     4621 caggggcagg aagatgatcg tcaaggcgga cccggcggcg acgtcctgtc ccccgccgat
     4681 cccgccgatg atccggccgc cggcgcagac ggtgttgacg ttgaagccca ggtcgacctc
     4741 cagggccgag agcacggcgg cgtcgagcat attgaccgct acttccttgt tggccgtcga
     4801 ggcgtagtag ccggtcgaga cttcgtgatg gttgggattt tcgagcatcg aggcgatcac
     4861 ccggggcgag ggctcgaaca tctgcccgtt catcaggtgc cggaccgtcc cctcctcgag
     4921 catgtcgacg tggagggacg tgatcccgcc gatggtgaaa tgagcctgga tcttcctctc
     4981 cttgaggatg gtccggatgt cgtccaagac gaccaggccg gccccgcttc cgacctggaa
     5041 gccgaacccg tcccggatca ccccggccgc cttcatcacc ccggtcacgt tggccgagac
     5101 ccgggcgttg aacgggttgg ccttggcctt ggggatgtcg agggtgcccg aaccgatgcc
     5161 ggagttcaag ccggcgcaag ccaccgggac gacgaaatcg accagctcca tggaaatccg
     5221 ggccggcagg acgagcctct cgctgacggt cccggcaagc aggcagacat aatcggcgaa
     5281 ctcggcgtcg gccgcgaaca gcccgatcgg gctgttcgcg gccgacgccg agttcgccga
     5341 ttatgtctgc ctgcttgccg ggaccgtcag cgagaggctc gtcctgccgg cccggatttc
     5401 catggagctg gtcgatttcg tcgtcccggt ggcttgcgcc ggcttgaact ccggcatcgg
     5461 ttcgggcacc ctcgacatcc ccaaggccaa ggccaacccg ttcaacgccc gggtctcggc
     5521 caacgtgacc ggggtgatga aggcggccgg ggtgatccgg gacgggttcg gcttccaggt
     5581 cggaagcggg gccggcctgg tcgtcttgga cgacatccgg accatcctca aggagaggaa
     5641 gatccaggct catttcacca tcggcgggat cacgtccctc cacgtcgaca tgctcgagga
     5701 ggggacggtc cggcacctga tgaacgggca gatgttcgag ccctcgcccc gggtgatcgc
     5761 ctcgatgctc gaaaatccca accatcacga agtctcgacc ggctactacg cctcgacggc
     5821 caacaaggaa gtagcggtca atatgctcga cgccgccgtg ctctcggccc tggaggtcga
     5881 cctgggcttc aacgtcaaca ccgtctgcgc cggcggccgg atcatcggcg ggatcggcgg
     5941 gggacaggac gtcgccgccg ggtccgcctt gacgatcatc ttcctgcccc tggccaccgg
     6001 caagaacggc aagggtttcc ccaaggtcgt ccaaaaagtc tacacccgga cgacgccggg
     6061 cgaggtgatc gacgtcgtcg tgaccgagga attcgtggcg gtcaatccgg ccggcgcctc
     6121 catttataaa gatgctctcc tggcaaacgg ggccgcggtt gggctgaaaa tgatctcgat
     6181 cgaggagctg cacgccaagt cggtcgaggc ggccaaggct ttcgggacca ttccgtccgc
     6241 gccgcaggcg accggcgaca tcgttcacgc cattgaatgg cgcgacggca cgctgcttga
     6301 cgtcattcgc aagaccgagt agaaaagggg ggaaatgaga gacgcggata aagaagacgc
     6361 gcgaaacggg ggagatcggt ccccattctt atgaatgggg accggggctc ccgtttcttt
     6421 tccgtcttgg gatcctgtct cctatttcaa gtttctcgta tcgccgctca tcgagggagt
     6481 cttcatgacc accgacttta agaaaatcga cgcgatcttc gccaaggccg aggccgacgg
     6541 ccgccgcttc ctgctcgagc ccgaaggcta tgccatgctc aagctggccg gcgtcgccgt
     6601 cccccgccac aagttcgtcc ccgctggaaa aaagctctcg gcggccgacg tcgccgccct
     6661 caaatctccg gccggcctcg tcctcaagat cgtcgccccg ctcatccagc acaaaaccga
     6721 cgtcggcggc gtcgccttcg tcaaggcctc cgcccccgcg gccaacgcgg ccatcaagaa
     6781 gatgatcgcc gaagttcccg gacgcttcct ggcttggtcc aaggctcatt ccgattccgc
     6841 ggccaggggg gccgctcccg ccgccgcagc ttcctcctct tcccccgccg gcctcaccgc
     6901 taaggacgtt gcggccgaca tccgaggggt gctcgtctgc gagcaggtcg ctttcgagaa
     6961 gttcggcttc ggctcggagc ttcttctcgg ggcccgcaat tcccgcgagt tcgggccggt
     7021 cgtgaccttc ggagccggag gcgtcgaggt tgaatatctc ggcgagcgcc tcaaggaagg
     7081 gacggccgcg gccatggcct cggcccatct tctcgcccca aaaaaaatcc tcgaccacct
     7141 ctccgcggcg gccgtttacg acaagctggt caagcccttc cgcggcaagc cggcccccct
     7201 caagccggtc gtcctccggg agacctacgc ccggttccag gccctgaccg cgcattattc
     7261 gccgttcggg ccggcctcgc gggttgtcat cgaggagatc gaggtcaatc ccttcgtcgt
     7321 ccgggccggc cgcctcgtgc cgctcgatct gctgtgccgc ttctcccgcg accacgtgga
     7381 cgtcaaaaac cgccccgtcg ccgggatccg cccgctgctt cggcccgcct cgatcggcgt
     7441 catcggcgtc tcggagaaaa tgaatctcgg gcatgtcatc ctcaacaacg tcatcgagat
     7501 gggctttccg aaggaccgcg tctacgtggt caaaccgggc ctcgccgaga tcgagggttg
     7561 ccggtgcgtc cccaccgtgg ccgacctgcc ggagacggtg gacatgttcg tcctcaccct
     7621 gagcgccgag cagtgcgagc cggtcatgcg cgatctcatc gcccacgaca aggcccgctc
     7681 ggtgatcatc atcgccggcg ggatggggga aaagtccggg acccagtcca tcgagaagaa
     7741 gatcgtcgac cttttggccg agagccgcaa ggccggccgg cccgcgcccg cggtcaacgg
     7801 cggaaactgc ctcgggatct acagccggcc gggctcttac gacacgacgt tcatcccccg
     7861 ctacaagctc cgctttccca aaaccgggat ccccggcctg gtctacgtca gccagagcgg
     7921 ggcgttcatg atcagccgga tttcccgcct ccagcgcttc gtcccgctct acggcatttc
     7981 catcggcaac cagatcgacc tgcgcgtctc tgattatctc cgctacctca aggatgagcc
     8041 tgaagcgaag gtcgtcgccc tctatatcga gggattcaag ccgggcgacg cgtacccgac
     8101 ggccgcggcg gcccgggaaa tcacccgagt tcccggccgg tacgtcattg cctacaagag
     8161 cggccgctcg ccggaggggc gtctggcgac ctccagccac acagcctccg tggccggcga
     8221 ctacggcatc tgcaaggccg tgcttgagga agccgggatc atcgtggccg acacgatcgc
     8281 cgagttcgaa agttaccttt ccggcgcgct gatgctcggc ggaaagcgct gggccggaaa
     8341 ccgggtcgtc atgatgagca cggccggatt cgaaagcgtc atcatgtcgg acaacctccg
     8401 gaacgggacg gggagcggtg gaaccctcga gctggccgtc tatacggacg aaacgaaacg
     8461 gaagctggag gaggccctgg cctccctcgg gatcgacaag ttccaagacg tccgcaatcc
     8521 gctcgacacg acgcccgtgg ccgacgacac ggtcttctcc gcctgcgccc aggccgtctt
     8581 ggaggatccc ggggtggatt gcgcggtcat ttcggtgcat ccgctgaccg ggtccctgca
     8641 gacgatcgct ccggccgaag ggcatcccca gaacgtctac gatccctcga gcgtcggccc
     8701 gaggttgatc gagattttca aggcgacgga caagccgttc gtcgtcagca tcgacgccgg
     8761 cgtcttgtac gacccgctgg ccgatctgct ccaggaggcc ggcatcccgg tgttccggga
     8821 ttgcgacgcg gcggtcaagt tcctgaggaa gttcgcggga gcggcgacga agcggtgagg
     8881 acgagggcgc atgcccaaag acaaggctca tccgttggcg tatctcagcg aaagcggggc
     8941 cttcctcatc agccggatct cccgccggcc ttggcttgag ccgctgtacg cgatttcgct
     9001 ggggagccaa gcggacctgc gcctctcgga ttatctgcgt tttctaagag acgagaccga
     9061 ggtgaaggtg gtcgcggtct acgtcgagag ctttatggcg ggcgacgggt atctggcggc
     9121 caaggcggtc cgggagatca cccggtcgcc cggacggagc gtgatcattt ataagggcgg
     9181 ccgttccccg gcgaaagaag gatcaggggc ggaggaattg gacggaatcg gggtggagag
     9241 gggaaaaaag tggacgcggg gggagggaag cgcggaaggg acggaggaga gagagcggag
     9301 cgagagagga gaatgggcag cggaaaaaag cgcggggatg agcgtgaggg cgggtgcgat
     9361 agccggcgcg agcgcgggga tggaaaagcg gatggatggg gggatggata agggagaaga
     9421 aaagaagccg gagaaaaaaa cggaggcgag gccgggggct gattacgagc ttttccgggc
     9481 tgttttcgag gacgcgggcg ccctcatcgc cgatacgatc gccgagttcg agagcttcgt
     9541 ttccggggct ttgctgttag gcggaaagcg cgtcggcggg aaccgcgtcg ccctgatgag
     9601 caacgccggg ttcgaatgcg tcgtcatgtc agacaacctc gggaacgggg aggaacggct
     9661 ggagccggcc gctttttcca ccgagacgat gcggcggatc ggggaggcgc tggccccgct
     9721 ggggatcaac aagatccagg acatccggaa tcccttggac gtgacgccgg tggccgacga
     9781 cgcggcgttc gccgcggccg cgggggcggt gctcgacgac ccgggggtgg attgcgcggt
     9841 catctcgcct cttcccttgg gcccggtgct tcagacgctg ccggcggggg aggggcacag
     9901 ggaggatatg gaaggcgagg ggagcatcgg gaagcggctg atcgagattt tcaaggcgac
     9961 cgacaagccg tttgtcgtca atgtcgattc gggggcggat tacgacccgc tgtgcgcgat
    10021 gctcgaggag gccgggatcc cggtgttcag ggattgcgac gcggcggtga ggtttatgag
    10081 aaagtgggtg gggaggagga ggatttggac gactccctct cgttctatga tttcccggac
    10141 atcgacaaga aacggatctc ctcggccaac ggccaggagc ggttgaatcg cgagatccgc
    10201 cggcgaagcc gggtcgtcgg cgtttttcca tccgtcgagt cctatatcag acttacgatc
    10261 tgctacctgg tcgaatatac ggaggactgg agcggggagc gaagctacat tcgagaggac
    10321 aagatcatca aatccttgga aaagcttcat gaactgacaa gtcaaatcgg gtgttgaaga
    10381 gagttacgat ggcccctaaa acgcaaacat ccgttgacac ggctgagcgt ccagtaccgt
    10441 tttccggttc ttccggccgt tcttaacata acccgaggtc cggtttagct ccctcggtgg
    10501 gggaatgact gggtctcatg ccggttctgg atgagagccg agggcgaaaa agcaaatacc
    10561 tatttttttc attgattgac ggggaaaaaa tcaatatgat gtgcggggtt cg
//
LOCUS       DAOOXH010000085        10565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_6756_length_10565_cov_6.156232, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000085 DAOOXH010000000
VERSION     DAOOXH010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10565
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6756_length_10565_cov_6.156232"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1170
                     /locus_tag="PKV64_09240"
     CDS             118..1170
                     /locus_tag="PKV64_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HPN17296.1"
                     /translation="MSSSSMSPAGKISLKPLKIGALRLDFPVVLAPLAGYSDLPFRTI
                     CRSLGATFATTEVMIDRFLLHESKLRRFILRTDAGDHPVGGQFMGSDPELMAQAARVV
                     RDLGFDVVDLNFACPVRKVVSRKRGGYLMTRPAEVLEIVRRVRDAVPDRPLMIKLRRS
                     FALEDAEAGAFWEIAGGAFEAGADALCVHARAVAQQYAGKAEWGFLESVKKAFPDRTI
                     IGSGDVSSAAEALRMISETGVDGAAAARGAIGNPWIFRQARQLAAGRPAPPPTVAEQR
                     EVLLRHYGLCLQAYGERKGVMTMRHLGIGYARVHPHPKRVRMAFVAVKTAEDWHGVLA
                     AHYAGEGDQRGSQARI"
     gene            complement(1143..2540)
                     /locus_tag="PKV64_09245"
     CDS             complement(1143..2540)
                     /locus_tag="PKV64_09245"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPN17297.1"
                     /translation="MDKKHAEGCIRIAAALFFAAVFALAGPESAWKDQVSFPRTSAGL
                     SVEAYFEAFNAGVPAMREFFSTHTAKSALPRASVEIRVERYRQMKERLGVLRPQKIVA
                     SREDFISVVAAAEKGQVVRLEFQFEPSAPYGLLGVRLEREEPEAEGAPPAEPKKDEAG
                     LLAAVSALVDEAVQKEEFSGVVLVAKDGTPVFERAYGLADREAGIPNRVDTRFNIGSI
                     NKSFTGLAVLRLAAENKLSLNDPVSLYLADYPNPDVAAKVRVRHLLHMTSGLGDFFGE
                     RYEATPKEKIRRLEDYLPLFADKPLEFEPGTREKYSNAGYIVLGLIIQKASGMDYAAF
                     MEERIFKPAGMADTGWIDKDAPLPERAVGYVREGAVLKPNTDTLPGRGSSAGGGYSTA
                     RDLLSYTTAIATGILPGDVEKMGQGLALAGGAPGLNAALEWDTRSGYVIIVLSNFGPP
                     SAEKIARQIRAWLPR"
     gene            2788..3852
                     /locus_tag="PKV64_09250"
     CDS             2788..3852
                     /locus_tag="PKV64_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17298.1"
                     /translation="MAGRKILGSLLIVLIGLPVLFGMIWAVGLVRATVSSEFLSDLPR
                     KIIADIPASIDDVFAATESESRWIDESARAWMRAARETGIRPGELMEKTGMLGWLQGE
                     LSTSLSRVGGIMRGEAPVEPILIDMRPLKAALLHPEMDRFFESLVQHLPPCDDQGLKV
                     WDDRLSAGFVRVEDLPACRPVDAASAQAVLRAERTREVEKMDDSVRVFDDVDSYPFGR
                     VGISRAVSTMSYLLFLIPALVILCGVLLANRTSAGRMRWGGISILAGSLPVVLMALLL
                     KRVVGWAAGGRWMHWSWGWRSDWDSILLDKLGWIPERIMSALFTPVFNTAIVVAVIGV
                     VLVALSYSTRQAAPAVPVKA"
     gene            4112..4918
                     /locus_tag="PKV64_09255"
     CDS             4112..4918
                     /locus_tag="PKV64_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17299.1"
                     /translation="MMWPFIKKKQPPETKPIEDGLKEKIASQLIFDPLTKRLKIKPNK
                     LEPLTTGVEAETKIILECPTCKEKLVKYSVNRFKCPHCRKWIYYRDHTLFTMEEYHRR
                     REEYFRRRQEEWQRQELKEGLESIGLTEKQFLLREQELAKKAGIAPTKISVTMSLFNE
                     FILKVKDQHEQEQKYRNLAIMLNTSGLNVFHILKAGAKAKLAAYKKDGIKNVYLLTGR
                     MCEVCMKLDGQVMTIAKALENMPIPIQTCKNHPYNDDMPFCICSYEPEYE"
     gene            complement(4991..9958)
                     /locus_tag="PKV64_09260"
     CDS             complement(4991..9958)
                     /locus_tag="PKV64_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPN17300.1"
                     /translation="MLSYGASRPMQFLKIRHAAEHNLKDVDVDIPHGRMTVVTGVSGS
                     GKSSLVFDVVYHEARRRYLETFSAHARQFLGKLQRPDAAMISGLAPAVALRQRTSAAG
                     PRSTVGTMTEIHDDLRLLFARCGAAPGGGRLSRRMFSFNTAEGACPSCRGLGVEDRID
                     PDLLISDPGRSLRQGALALTTPNGYIIYSQVTMDVLDRVCRAHGFSVDVPWRDLTGAQ
                     RDIVLNGSDKIRIPYGKHPLESRLRWKGIVAKPREEGVYKGILPVMDAILKAKRNKNI
                     LRFARTLPCRACGGRRLRPEPLRVTVGGRTIADLSELSLRAFDAAVREMSFAPPDRAA
                     GEAIRASLQKKTEFLKRLGLGYLRLDRPADSLSGGEIQRLRLAGMAGSGLRGVLLVLD
                     EPTAGLHPADTARLLDLLTEIRNNGNTILVVEHDEDVMRAADHLIDLGPGPGEAGGRV
                     LFSGPPSEIVRLPPGSSPTRDFLAGGRGPAPSPAAAPVPPLAPSRKPSPATGLLRVRG
                     AKMHNLRSVDVEFRLGALNVVTGVSGAGKSTLVRHILAARLRSGRLGPGPDAEGIEIE
                     GRAGRLVEIDASLIGRTPRSNPATYTNISDRIRDLFAALPEAQARGFGKSRFSFNVPG
                     GRCETCSGAGLLHIGMHFLGDVDVVCPECEGRRFNPETLEIRDRGKNIHDVLEMSVAE
                     AAAFYAGTPKLSSILEILKRLGLGYLNLGQSSTTLSGGEAQRVKLASELGKTISGPAV
                     YILDEPTTGLHRADIENLLAALRDLVALGRTIIAIEHHPDVIRAAGLVVDLGPGSGED
                     GGRVVARGTPAEIAARADSATGRALRGEYEIQAARRAEAPPKPEAEGPLVLEGVTTHN
                     LRGIDARFPFRKLTVVSGPSGGGKSSLVFDTLFAAARQKFIENFSAYVRTLIDKGVRP
                     DFAAARGLTPPVAVGRSAAVPHPRSTVGTMTEIHDYYRLLFSRAGVQPPGSNGRTLSA
                     SMFSFNHEQGACPACKGLGTMTVVDPERLITDPQKPLPGGAMDGTKTGRFYGDPHGQH
                     MAALLAAGAAAGIDFLKPYEDLSVEAKEIALRGTGDRPYDIVWSYRRGARAGDFKFRG
                     PWKGLAALVGEEYERKHADRRGEAMRILMKDDPCPACGGRRLKPASLAVTYRGLSIAD
                     LSALSVGRSIEFFENSGGARPGAGVLDAKSAAVTAAVRGEILRRLRLLKDVGLDYLQV
                     DRPSSTLSGGEAQRIRLAGLLGVKLTGVTFVLDEPTLGLHPRDTADLLGLIRELTAEG
                     NTVVAVEHDRDVIRAADFVIDLGPGAGREGGLIVAEGTPADIEANPSSVTGPYLRSLG
                     TVPEPLTGTAGPPLEISGARAHNLRNIDVSIPSGVLTAVTGVSGSGKTSLVFDVLLAS
                     RRAGRAVSCDRIGGLERFARVVPVGPEGTAESAAGIPLTVLGVFDAVRGLFAATAEAE
                     ARGFGRPHFSYLTPQGRCPECGGTGRKTVSLDFLADVSEPCPECGGRRYQPAILEVRW
                     SGRTIADILELTAAEGAAVFSGQPKIAAALTLLADIGLGYLRLGQGLDTLSSGERQRL
                     NLAAELSGRTEGRILYLFDEPTTGLHPSDVERLLRLFGKLLSAGHTIIAVEHDLDLIS
                     RAGHIIDLGPEGGDRGGSLVVQGPPAAVAACPSSHTGAALRAFLR"
     gene            complement(10021..10356)
                     /locus_tag="PKV64_09265"
     CDS             complement(10021..10356)
                     /locus_tag="PKV64_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17301.1"
                     /translation="MAAELAADLFWSGGRAFRYNGEAMLGSLKGRALGFAPIVPGLAA
                     FASAALIAGLGLSIQSIRPSSPSGDDLILDGGFEGDPRSFPFLPRPGDPGGLFLQDEF
                     SRGPGEYSL"
BASE COUNT         1889 a         3255 c         3598 g         1823 t
ORIGIN      
        1 ggaaaatcga gcctcaaggc gccgattttc aggggcttcc ggaagcccgt ccgcaagcac
       61 taccggttcg gctgaggagc ggaaaagaat cccgcccggg ccgcggccgc agcagccatg
      121 tcgtcgtcgt caatgtcccc ggccggaaag atctccctga agcccctgaa aatcggcgcc
      181 ttgaggctcg attttccggt cgtcctggcc ccgctggccg gctacagcga cctgccgttc
      241 cggacgatct gccgctcgct cggggcgacg ttcgccacga ccgaggtgat gatcgaccgc
      301 ttcctcctcc acgaaagcaa gctccgccgc ttcatcctga ggaccgacgc cggcgatcat
      361 ccggtcggcg ggcagttcat gggcagcgac ccggagctga tggcccaagc cgcccgggtc
      421 gtccgcgacc tgggcttcga cgtcgtcgac ctcaatttcg catgtcccgt ccgcaaggtc
      481 gtcagccgga agcgcggcgg atacttgatg acccggccgg cggaggtcct ggagatcgtc
      541 cgccgggtcc gcgacgccgt ccccgaccgg cccctgatga tcaagctccg ccggtccttc
      601 gccctcgagg acgccgaggc cggggccttt tgggaaatcg cggggggagc tttcgaagcc
      661 ggcgcggacg ccctctgcgt tcatgcccgg gccgtcgccc agcagtacgc gggaaaggcc
      721 gaatggggat ttctcgaatc cgtgaagaaa gcgttccccg accggacgat catcggttcg
      781 ggcgacgtct cctcggcggc cgaggcgctc cggatgatct ccgagacggg ggtggacgga
      841 gccgccgcgg cccgcggggc catcggcaat ccctggattt tccgccaggc ccgccagttg
      901 gccgccggcc ggccggcccc gccgccgacg gtcgccgagc agcgcgaagt ccttctccgc
      961 cactacggcc tctgcctcca agcgtacggc gagcgcaagg gcgtgatgac gatgcgccac
     1021 ctggggatcg gctacgcccg cgtccatccc catcccaagc gcgtccggat ggccttcgtg
     1081 gccgtcaaga cggcggagga ttggcacggc gtcctggccg cccattacgc cggggaaggg
     1141 gatcagcgcg gcagccaggc gcggatctga cgggcgattt tctcggcgct tggggggccg
     1201 aagttcgata ggacgatgat cacgtagccg ctccgcgtat cccattccag ggcggcgttg
     1261 aggccgggcg caccgccggc gagggccagg ccttggccca tcttctcgac atcgccgggc
     1321 aggatgcccg tagcgatggc ggtcgtgtag ctcagcaggt cgcgggccgt ggaatatccg
     1381 cctccggcgg agctaccccg cccgggaagg gtgtcggtgt tgggcttcag gacggcgccc
     1441 tcgcggacat agccgacggc ccgctcggga agaggggcat ccttgtcgat ccagcccgta
     1501 tcggccatcc cggcgggctt gaagatccgc tcttccatga aggcggcgta atccatcccg
     1561 gaggctttct ggatgatcaa gcccaggacg atgtagccgg cgtttgaata tttctcccgc
     1621 gtgccgggct cgaattccag cggcttgtcg gcgaacaggg ggaggtaatc ctccagccgc
     1681 cggatctttt ccttcggcgt cgcttcgtag cgctctccga aaaagtcccc caagcccgag
     1741 gtcatgtgga ggagatggcg gacccgaacc ttggccgcga cgtccggatt cggataatcg
     1801 gccaggtaga gcgagaccgg gtcgttgagg gagagcttgt tttcggccgc taaccggagg
     1861 accgccaggc cggtgaacga cttattgatc gagccgatgt tgaatcgcgt gtcgacgcgg
     1921 ttggggattc cggcttcccg gtcggccagg ccgtaagccc gctcgaagac gggagttccg
     1981 tccttggcga cgaggaccac tcccgagaat tcttcctttt ggacggcctc gtcaaccagg
     2041 gccgagacgg cggccaggag cccggcctcg tcctttttcg gctcggccgg cggcgcgcct
     2101 tccgcctccg gctcttctct ctcgaggcgc acgccgagga gcccgtaggg ggcggagggc
     2161 tcgaattgga attccagccg gacgacctgc cctttttccg cggcggcgac gacactgatg
     2221 aagtcttccc gcgaagcgac gatcttttgg ggacgaagca ccccgaggcg ctccttcatc
     2281 tgccggtagc gctccacccg gatctcgacg gacgctctcg gcagggcgga tttcgccgta
     2341 tgggtggaaa agaactccct catggccggg actccggcgt tgaacgcctc gaaataggct
     2401 tcgacgctga gcccggcgga cgtccgcgga aaggaaacct ggtctttcca ggcggactcc
     2461 gggccggcca aggcgaaaac cgcggcgaaa aacaacgcgg ccgcgatccg gatgcatcct
     2521 tcggcgtgct ttttgtccat ggccgttatt taccagatcg gcgggcgggc gtaaagcgcc
     2581 ggccgccccg ggggatgcgg cccggcgatc atatcccttt ccgaggaggg gaggccgaga
     2641 tgaaggagag gattctctta agccggcgcg aaacccgacg gaggcaagcg gatcgcgcgg
     2701 gccgcttaga gggggctgga actccgcggc cgatttttcc gtataatagg gcggaaaggg
     2761 tcttatttct cggctggagg caggcaaatg gccggacgta aaattctcgg ttctcttctt
     2821 atcgttttga tcgggctccc cgttttgttc ggaatgatct gggcggtcgg cctggtccgg
     2881 gcgaccgtct cctcggaatt cctctccgac ctgccccgga agatcatcgc cgacatcccg
     2941 gcttcgatcg acgacgtgtt cgccgcgact gagtccgaaa gccggtggat agacgagtcg
     3001 gcccgggcct ggatgcgggc cgcgcgcgaa accggcatta ggccgggcga gctgatggaa
     3061 aaaaccggga tgctcggctg gctgcaaggc gagctttcga cctcgctcag ccgggtcggc
     3121 gggatcatgc gcggcgaggc gccggtcgag cccatcctga tcgacatgcg gcctctgaaa
     3181 gcggctctct tgcaccccga gatggaccgg ttcttcgaga gcctggtcca gcacctgccg
     3241 ccttgcgacg accagggcct gaaagtctgg gatgaccgcc tgtccgccgg cttcgtccgg
     3301 gtcgaggatc tcccggcctg ccggccggtc gacgcggcct ccgcccaagc ggtcctccgc
     3361 gcggaacgga cgcgcgaagt cgaaaaaatg gacgattcgg tccgggtttt cgacgatgtc
     3421 gatagctatc ccttcgggcg cgtcggcatc tcccgggccg tgtcgacgat gtcctacttg
     3481 ctcttcctga ttccggccct ggtcatcctc tgcggcgttc tcctggccaa ccggacctcg
     3541 gccggacgga tgcgctgggg cgggatctcg atcctggccg gaagccttcc cgtggtgctg
     3601 atggccctcc tgctcaagcg ggtcgtcggc tgggcggccg gcggccggtg gatgcattgg
     3661 tcatggggtt ggcggagcga ttgggactcg attctcttgg acaagctcgg ttggattccc
     3721 gagcggatca tgagcgccct cttcacgccg gtcttcaaca cggccatcgt cgtcgccgtc
     3781 atcggcgtcg tcctggtcgc cctgtcgtat tcgacccgcc aggcggctcc ggccgtcccg
     3841 gtcaaggcct gaaacccgag caagcggagg ggcggggacg cggacggcgt cgctcgcggc
     3901 ggcgaggctg gtggttgtcc aaaatggata ctagttttat attcgtatta ttcgtagggg
     3961 ttcggtttgg gcggttttat agcacttgaa tacgctggaa gtagcaggaa agcatgaggg
     4021 tttcttgcgc gaaaacggga ttaaaaaatt ttgatgatta tctagccggg attcgtttta
     4081 tagctcgttt gttcagctga tctgggagga aatgatgtgg ccattcatta agaaaaagca
     4141 gccgcctgaa acgaagccca ttgaagacgg gctcaaggaa aagatcgcgt cgcaattgat
     4201 ctttgatccg ttaactaaac ggcttaaaat aaagcccaat aagttggagc ctttgactac
     4261 aggagtcgaa gctgagacga aaataatcct ggaatgtcca acctgcaaag agaaattggt
     4321 taagtactcg gttaataggt ttaagtgccc tcactgccga aaatggattt actaccgaga
     4381 tcatacgctc tttacgatgg aagagtacca cagacgccga gaggaatatt ttcgaaggag
     4441 gcaggaagaa tggcaacggc aagaattgaa agagggattg gaatccatcg gcttgaccga
     4501 gaagcaattt ctgttaagag aacaggagtt ggcgaaaaag gctggaatag ctccgacaaa
     4561 aatatcagta actatgagtt tattcaatga atttattttg aaggtcaaag accaacatga
     4621 gcaagagcag aaatatcgta acctcgccat catgctgaac acgagcggat taaatgtatt
     4681 ccatattttg aaggcgggcg cgaaggcgaa attagcagct tataagaaag atgggatcaa
     4741 gaacgtttat cttctcacag gtaggatgtg tgaagtttgt atgaaattgg atggccaagt
     4801 gatgacaatt gcgaaagctc tagagaatat gcctattccg attcaaacat gtaaaaatca
     4861 tccctataac gacgatatgc ccttttgcat atgcagctat gagccggagt acgagtgata
     4921 gaccgggggc gtcaggaatc agagcgagta tagtcggggt accggtcggc atctatgctt
     4981 tggccaataa ttatcgcagg aaagcccgga gggccgctcc cgtgtgggac gacggacagg
     5041 ccgcgaccgc ggccggcggc ccctggacga cgagcgatcc gccacggtcg ccgccttccg
     5101 gccccaggtc gatgatatgg ccggcccggg agatcaaatc gaggtcgtgc tcgacggcga
     5161 tgatcgtatg tccggcggaa agaagcttcc cgaagagccg cagcagccgc tcgacgtcgg
     5221 agggatgaag cccggtcgtc ggttcgtcga atagataaag gatccggccc tccgtccggc
     5281 ccgacaattc cgcggccaga ttgaggcgct ggcgctcgcc cgacgaaagc gtgtcgaggc
     5341 cctggccgag gcggaggtag ccgagcccga tatcggcgag cagggttaag gcggcggcga
     5401 ttttcggttg tccggaaaaa accgccgcgc cctcggccgc ggtcagctcg agaatatcgg
     5461 cgatcgtccg gcccgaccag cggacctcca gaatcgcggg ttgataccgc ctcccgccgc
     5521 attccgggca aggctccgag acgtcggcta aaaaatccag gctcaccgtc ttccggcccg
     5581 tgccgccgca ttcgggacac cggccctggg gcgtgagata cgaaaaatgc ggccggccga
     5641 atcccctggc ttcggcctcc gcggtcgcgg cgaacagtcc ccggacggcg tcgaagacgc
     5701 cgaggacggt cagggggatg ccggccgcgc tttcggccgt cccttccgga ccgacgggga
     5761 cgacccgggc gaaccgttcg agaccgccga tccggtcgca tgaaacggcc cggcccgccc
     5821 gccgcgaagc caggaggacg tcgaagacga ggctcgtttt cccgcttccg gagacgccgg
     5881 tcacggccgt caggacgccc gacggaatcg agacgtcgat gttccggagg ttgtgagccc
     5941 gggccccgga gatttccagg ggagggccgg cggttcccgt caacggctcg ggaaccgtcc
     6001 ccaaggagcg gagatacggc ccggtcaccg aggagggatt ggcttcgata tcggccggcg
     6061 tcccctcggc gacgatgagg ccgccctcgc ggcccgcccc gggtcccagg tcgatcacga
     6121 aatcggccgc ccggatgaca tcgcggtcgt gctccacggc cacgacggtg ttgccctcgg
     6181 ccgtgagctc ccggatcaat ccgaggagat cggccgtgtc ccgcggatga aggcccagcg
     6241 tcggctcgtc gaggacgaag gtgaccccgg tgagcttgac gccgagcagg ccggccagcc
     6301 ggatccgctg ggcctcgccg cccgagagcg tggaggaagg gcggtcgacc tggagataat
     6361 ccaggccgac gtctttgagg aggcggagac ggcggaggat ctcccctcgg acggccgcgg
     6421 tcacggcggc cgatttcgcg tccaagacgc cggccccggg gcgcgctccg cccgaatttt
     6481 caaaaaattc gatgctccgg cccacgctga gggccgaaag atccgcgatg ctcagcccgc
     6541 ggtaagtcac ggccagcgag gcgggcttga ggcggcggcc gccgcaggcc gggcagggat
     6601 cgtccttcat caggatcctc atcgcctcgc cccggcggtc ggcgtgcttc cgctcgtatt
     6661 cctcgccgac gagggcggcc agccccttcc acggcccccg gaacttgaaa tccccggccc
     6721 tcgcgccgcg ccggtacgac caaacgatgt cgtagggacg gtcgcccgtt ccccgaaggg
     6781 cgatctcttt cgcctcgacc gagagatcct cgtacggctt caggaaatcg attccggccg
     6841 cggctcccgc ggccaggagc gcggccatgt gctggccgtg aggatcgccg tagaagcggc
     6901 cggttttcgt cccgtccatg gctccgccgg gaagcggttt ttggggatcg gtgatcaacc
     6961 gttccggatc gacgacggtc atcgttccca ggcccttgca ggcggggcag gcgccctgtt
     7021 cgtggttgaa ggagaacatc gaggctgaaa gcgttcggcc gttcgatccc ggaggctgaa
     7081 ccccggcccg ggagaacagc agccggtagt aatcgtggat ttcggtcatc gtccccaccg
     7141 tcgagcgggg atgggggacg gcggccgacc ggccgacggc gaccggcggc gtcaatcccc
     7201 gggccgcggc gaaatcgggc cggacgccct tgtcgatcag ggtccggacg taggccgaaa
     7261 aattctcgat gaatttctga cgggcggcgg cgaaaagcgt gtcgaagacc agcgaggatt
     7321 ttccgcctcc ggacgggccg ctgacgaccg tcagcttgcg gaaggggaag cgggcgtcga
     7381 tcccgcggag gttgtgggtc gtcacgccct cgaggacgag cggcccctcg gcctccggct
     7441 tgggcggagc ttccgcccgg cgggcggctt ggatttcgta ttcgccccga agggcccggc
     7501 cggtcgccga gtccgcgcgg gccgcgatct ccgccggcgt tcctcgggcc acgacccgcc
     7561 cgccgtcctc gccgcttccc ggcccgaggt cgacgaccag gccggccgcc cggatgacgt
     7621 ccgggtggtg ctcgatggcg atgatcgtcc ggccgagggc gaccaggtcc cggagggcgg
     7681 ccagaagatt ttcgatgtcg gcccggtgaa gcccggtcgt cggctcgtcc aaaatgtaga
     7741 cggccggccc cgagatcgtc ttgcccagct cggaggccag cttgacccgc tgggcttcgc
     7801 ccccggaaag cgtcgtcgag ctttggccga gattgagata gcccaggccg aggcgcttga
     7861 gaatctccag gatggaggaa agcttgggtg ttccggcgta gaaggcggcc gcctcggcca
     7921 cgctcatctc caggacgtcg tggatgtttt ttccccggtc gcggatctcc agcgtttcag
     7981 gattgaaccg gcggccctcg cattcggggc agacgacatc cacgtccccc aggaagtgca
     8041 tgccgatgtg gaggagtccg gcgccgctgc acgtttcgca ccggccgccc gggacgttga
     8101 aagagaagcg gcttttcccg aacccgcggg cttgggcctc gggcagggcg gcgaacagat
     8161 cgcggatccg gtcggagatg ttcgtatagg tcgcggggtt ggagcggggc gtccggccga
     8221 tgagggaggc gtcgatttcg accaggcggc cggcccgccc ttcgatttcg atgccctcgg
     8281 cgtccgggcc ggggcccagc cgcccgcttc ggagccgggc ggccaggatg tggcggacga
     8341 gagtggattt cccggctccg gacacgccgg tcacgacgtt gagggcgccc aggcggaact
     8401 cgacgtccac cgagcggagg ttgtgcatct tcgctccgcg gacgcggagg aggccggttg
     8461 ccggggaggg cttgcgggat ggagcaagag gcggaactgg ggccgccgcc ggagacggag
     8521 cggggccccg gccgccggcc aggaagtcgc gggtcgggct cgagcccggc ggaagccgga
     8581 cgatttccga gggcggtccg ctgaaaagaa cccggccgcc ggcctctccc ggccccgggc
     8641 cgagatcgat caggtgatcg gccgcccgca tcacgtcctc gtcgtgctcg acgacgagga
     8701 tggtgttgcc gttgttccgg atctccgtta aaagatccag cagccgggcc gtgtcggccg
     8761 gatgcaagcc ggcggtcggc tcgtcgagga cgaggaggac gccccgaagc ccgcttccgg
     8821 ccatcccggc caatcggagg cgctggattt cgccgcccga aagggagtcc gccggccggt
     8881 caaggcggag atagccgagg cccagccgtt tgagaaattc tgtttttttt tggagcgagg
     8941 cgcggatggc ttcgccggcc gcccggtccg ggggagcgaa gctcatctcc cggacggccg
     9001 cgtcgaacgc ccgcaggctc aattcgctga gatcggcgat cgtccggccc ccgacggtca
     9061 cccgcagcgg ctcgggccgc agtcgacggc ctccgcaagc ccggcagggg agggtgcggg
     9121 cgaaacggag aatgttctta ttccgcttgg ccttcaggat ggcgtccatc accggcagga
     9181 tgcccttgta gacgccctct tcccggggct tggcgacgat gcccttccag cgcaggcggg
     9241 attccagcgg gtgtttcccg taggggatgc ggatcttgtc gcttccgttg aggacgatgt
     9301 ccctctgggc cccggtcagg tcgcgccagg gaacatccac ggaaaaacca tgggcccggc
     9361 agacgcggtc caggacgtcc attgtcactt gggagtagat gatgtagccg ttgggggtcg
     9421 tcaaggcgag ggcgccttgg cggaggctgc ggccggggtc ggaaatcaag agatccgggt
     9481 cgatccggtc ctccacgccc agcccgcgac aggacgggca ggcgccctcg gccgtattga
     9541 aggaaaacat ccggcgggaa agacgtccgc cgccgggcgc ggcgccgcaa cgggcgaaaa
     9601 gaaggcggag gtcgtcgtgg atctcggtca tcgtcccgac ggtggagcgc ggcccggccg
     9661 ccgacgtccg ctggcggagg gccacggccg gggcgaggcc gctgatcatt gccgcgtcgg
     9721 gccgctggag cttgcccagg aattggcggg cgtgggccga gaacgtttcc aagtagcggc
     9781 ggcgggcctc gtgatagacg acgtcgaaga cgagcgagga tttgcccgag ccggacaccc
     9841 cggtgacgac ggtcatccgg ccgtggggga tgtcgacgtc gacgtccttg aggttatgct
     9901 cggcggcgtg ccggatcttg aggaattgca tgggccggga agcgccatac gatagcatcg
     9961 gccttggagg aggtcaagcc tcttttcaat taaaaaaagc gcatgaagcc ttgattttag
    10021 tcacagggaa tattctcccg gcccgcggga gaattcatcc tgaaggaaaa gcccgcccgg
    10081 gtcgcccggc cgggggagga acgggaaaga gcgcggatcg ccctcaaagc cgccgtcgag
    10141 gataagatcg tcgccggacg gagaggacgg tcggatggac tggatggaaa gaccgaggcc
    10201 ggcgatcaag gcggccgagg cgaaagcggc caggcccggg acgatcgggg caaatcccaa
    10261 cgcccttccc ttcaagctcc caagcatcgc ctctccatta taccggaaag cgcggccgcc
    10321 gctccagaac aaatcggcgg cgagctccgc cgccattctt tcttttattt atgaaaatca
    10381 gtcaaaaaga gacattgtcg cggccggaaa attcaatccc cctagccgcg ggaggcgtcc
    10441 aagatgtcgt gacccgggac cgccgcttga caggcggaaa gatttccgca taatgatctt
    10501 tcgattcccg cccccggggg gtttagaggt ttttgcgatg agaaagcaga cgttgctcgg
    10561 atgga
//
LOCUS       DAOOXH010000086        10493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_6830_length_10493_cov_7.305806, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000086 DAOOXH010000000
VERSION     DAOOXH010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 10493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10493
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6830_length_10493_cov_7.305806"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            400..939
                     /locus_tag="PKV64_09270"
     CDS             400..939
                     /locus_tag="PKV64_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HPN17302.1"
                     /translation="MIPSKVRDLMNEQVKNELESFYIYLSMAAYFHEQNLDGMAHWMR
                     VQAHEETTHAMKFFDHIIDRGGEAVLLDLKQLKKTWSSPLEAWKDAYAHEQFITSKIH
                     AIVKVVRAENDYSAEPLMDWFVKEQIEEEKNTDGIARQLERVGESKDGLYMLDKELAA
                     RPWPAGSCFDPASYNAPGA"
     gene            1022..2308
                     /locus_tag="PKV64_09275"
     CDS             1022..2308
                     /locus_tag="PKV64_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN17303.1"
                     /translation="MLRVGRLSRRSFLKTAAAAAAGAVLVPRRVLGGPGILPPSDELT
                     KAVIGVGGMGLGHMGYEGARLLAVCDVDASHLQTALDAAGPGVRGYRDFREILARPDI
                     DIVHIATPPHWHALMSIAAVEAGKDVWCEKPMTRTIGEGIRVVDAVRRTGRMFRVNTW
                     FRFQDRFYGFGTPVKPLKKALRGGLLGWPVRAVLNAATGFDWKFYWSGKTYLPPAPVP
                     PELDYDFWLGPAPFKPYHPHRVHQTFRGYWDYDGGGLGDMGMHYLDPVQDLLDKDGTS
                     PVEITADAPQQHPDACGRWRRVCLKYADGCEIVLDGEGREADAAFLQGPGGRIFRGLH
                     SDIPDFLEKIAALPDPDPGVTDFSESVRTRVKFALNEENGHRSCTLVNLAKIAVRLGR
                     PLRFDPDGQRFVNDEEADRLIDEPARSPWHREGFGR"
     gene            2305..5094
                     /locus_tag="PKV64_09280"
     CDS             2305..5094
                     /locus_tag="PKV64_09280"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPN17304.1"
                     /translation="MRKRCRAPAAVFLIAFLSLTAAGRDPAGDLAGRVSALLLRFPAD
                     SAAEREAAAAELIRIGPGAVADLCGRLAAPGTADDSLVRYALDALAVRAGRPGAEAER
                     KAAADGILAALTIPRDQENAVFLISLLQKIGRLEIVKPLGRFLTRPDLAEPAVRALTA
                     TGLPEAAAALAGALATARDETAVAIIRGLGDLRAREAGPALLRLAEGNDPDLRIAALD
                     ALADIGDPAAREALERVRVAAPRAERSAAASRLLRFGRRLAEEGRPDMAADIARPFLS
                     SYASPGEEHLRAAALTLFADAAGESEVRDAVRTASRSPDAAYRRRALDIAAGPRIPWS
                     AGEWIGLLERAEPDAAVDVIRLLARKNEASALPAIRDILRAGEGAVRREAALAAARLG
                     GAAVWDEIVPLFLSGGPADAAAAKKAVLLFPSESLVPAVAGSIFSAPPAGRVALLEIL
                     ADRSAVSTAGAVFCLSWNRDERVRTAALAALEQTVTAEDLPAVVALLLQASTPSETLL
                     LQNAAVAAARRIPDPEARAGRILEWLGRIVGPRRIDLLRPLSRIGGAAALEAVREEIR
                     KGDPQARAVAVFTLAAWPDESAVPDLFRLARSASDKKTRYLALQGLARLIPGSSSAPD
                     ARLAALSEALAAAVEADEKALILDAIAAARVPEGLGTILRFLDDPDLRSRAARAVFRL
                     VMPAAGYGGLSGLAAAAALKRAVPYIDYEFDRVPAERRALELLVQEGFQPLFNGKDLT
                     GWKGLAADPPAWTKMTLEEIARAQAEADASMRAHWRVVDGVLSFDGRGGNLCTARDYG
                     DFEMFVDWRIGPGGDSGLYLRGSPQVQIWDPDQRPEGSGGLYNNRIHPRSPAVRADRP
                     AGEWNTFFIRMTGERVTVDLNGVRVVDDVVMENYWERDKPIYPAGQIELQAHDTPLEF
                     KNILLREIPAGRTGS"
     gene            5203..6597
                     /locus_tag="PKV64_09285"
     CDS             5203..6597
                     /locus_tag="PKV64_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN17305.1"
                     /translation="MPDPKSPPENGLSRRAFLGTAATAAAGFMIVPRKVLGGPGQKAP
                     SDTLNIGCVAVGGKGFSDVQDVSSENIVALCDVDDQKMAAFLTSDEHPYDKKMKYEKA
                     AKYRDWRKMLETEKNLDAITVSTPDHNHAVIAMAAMKMGKHVFVQKPLTHTVKEARLL
                     EEEAKKRNLVTQMGNQGHAREGARLICEWIWDGAIGDVTEVHVWTDRPIWPQGVEAPK
                     EIPSVPPNLDWDIWLGPAPWRPYHPALTHFVWRGWFDYGTGALGDMGAHLIDMPYWAL
                     KLGHPLTVQASSTRPTKDSYPVAEIVTYEFPARGSMPPVKMVWYDGGLTPPRPVVSVL
                     ENGRMMGDEDGGGVLFVGQKGMIMCGTYGDNPRILPESLMQSYQRPEKTIPRSPGIAA
                     EWIEAIKKGTKSTTDIPTYSGPLTEVMLLGNIAMRFRQSKTILQWDPVKMEFPNLPEA
                     NAFVHKEYRIGWSL"
     gene            6698..7435
                     /locus_tag="PKV64_09290"
     CDS             6698..7435
                     /locus_tag="PKV64_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014036314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPN17306.1"
                     /translation="MILSAFVVAVSGGNPPEDLRTWAIHDPARPRPPAVDPGPAGPSV
                     PAPAGAAVLFDGRDLSRWTAIGGGPAGWKVENGFMEVVKGTGSIRTREEFGDCRLHVE
                     WAAPAEVRGSGQGRGNSGVFLMDRYEIQILDGWENPTYADGMAAAVYGQYPPLVDACR
                     RPGEWQTYDIVFRAPRFDASGGLLSPARMTVFHNGILVQDDVPLTGPTAHKARPPYAV
                     HPEKQPLSLQDHGHPVRFRNIWIRPPS"
     gene            7580..9262
                     /gene="ettA"
                     /locus_tag="PKV64_09295"
     CDS             7580..9262
                     /gene="ettA"
                     /locus_tag="PKV64_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HPN17307.1"
                     /translation="MPSDTQVIYSMIGVGRIHPPNRQVLKDISLGFYYGAKIGVLGLN
                     GSGKSTLLRIMAGLDPDYIGEIAKSKGYTVGLLEQDPKLDPGKTVIEIVREGVQPIVD
                     LLARYDAVNEKFADPGADMDALMAQQARLLEEIEKHDAWNLDNHLEIAMEALRCPPPD
                     TPVTILSGGERRRVALTRLLLMEPDILLLDEPTNHLDAESVAWLERHLQEYKGTVIAV
                     THDRYFLDNAAGWILELDRGHGIPWKGNYSSWLEQKTARLAVEEKTESRRRRTLAREL
                     EWIRMSPKARQAKGKARVTAYEKLLSREYDRYRDEQEIPIPQGNRLGDIVIEAEGVTK
                     AYGDRLLIDDLSFRVPPGSIVGVIGPNGAGKTTLLRLITGMETPDAGRIRIGETVKLV
                     YADQSRRLDPDRTVFEAVSGGAETMIIGGREVNARAYCASFGFAGTDQQKKTAVLSGG
                     ERNRVHLARAMAEGGNVLLLDEPTNDLDVNTLRALEDALESFAGSAVIVSHDRWFLDR
                     VATHILAFEEDGSARWIDGNYTAYREDLRRRKGAAADQPHRLKHKTLKRSTG"
     gene            complement(9290..>10493)
                     /locus_tag="PKV64_09300"
     CDS             complement(9290..>10493)
                     /locus_tag="PKV64_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPN17308.1"
                     /translation="EDRMWLHVVVKDSGIGIPPEKIKQIFDPFAQVDSSTTRVYGGTG
                     LGLSIVSQLVELMGGKIWVESELGIGSQFHFTMPLALQQGGETEVVPLQFEDIKGTSV
                     LAVDDNETNRRILHEMLTNWHMRPTVVADGKTALKALREAREHGRSYQLILIDANMPE
                     MDGYALAEEIKKHPELGNAFIMMLSSAGFRGEAARCRKLGLSAYLTKPIKQSYLLDAI
                     MLALGAGVRKPGDAQAPFITRHSLSRARGQYKILLAEDNVINQKLAVRILERREHQVK
                     VAANGVEALEFLEKEPFDLVLMDVQMPKMDGYQATAEIRKAEAGTDRHLPIVAMTAHA
                     MKGDREKCLEAGMDDYLSKPLKPYDLLKKIEYVVNRFRKELKIIEKRIDGQDEENLEN
                     APEPGEEI"
BASE COUNT         1777 a         3416 c         3445 g         1855 t
ORIGIN      
        1 gcgctttaaa aggacgggga ctcccatcct ccagggtagt cagatcatag ggatcgaacc
       61 gtcgtttcca acgataaaac gtttgagccg atatatcgaa atgacgacag gtcaaacggg
      121 cgttctcatg cctggcataa tggtccatcc atttcaatcg ggccgccgct gttgaagtga
      181 tcgttcccct tcggatcaag tctcgaaggt actcagatcc ggggatcagg gagccatagg
      241 aggttgtcgg ttgtagatac atagcaacac ctatcataga tcaaggtgtc actaatcatt
      301 ctgaagtagt tcagggggcg gcgcgcggtt gacaaaaccg ctcggccgcc gtactattca
      361 gattgtaatg attacaaatt gatgaaaaag gagaatccga tgatcccgtc caaagtccgc
      421 gatctcatga acgaacaggt caagaatgag ctggagtcct tttacattta tctttccatg
      481 gccgcctatt ttcacgagca gaacctcgac ggcatggccc attggatgcg cgtccaagcc
      541 cacgaggaaa cgacccacgc gatgaaattc ttcgaccaca tcatcgaccg gggcggcgag
      601 gcggtccttc tcgatctcaa gcagctcaaa aagacatggt cctcccccct ggaagcctgg
      661 aaggacgcct acgcccacga gcagttcatc acctcaaaga tccacgccat cgtcaaggtc
      721 gtccgggccg agaacgatta ctccgccgag ccgctgatgg actggttcgt caaggagcag
      781 atcgaggagg agaaaaacac ggacgggatc gcccgccagc tggaacgagt cggagagagc
      841 aaggacggct tgtacatgct tgacaaggag ctggccgccc ggccctggcc ggctggatcg
      901 tgcttcgatc cggcgtccta caacgccccg ggcgcctgat aacggccgcc ggcgcggttg
      961 attcctccgg ttccttcgga ataagataac cccggtccgc attttgtttc cggaggtctc
     1021 catgctccgc gtcggccgcc tgtcccggcg ttccttcctt aaaaccgcgg ccgcggccgc
     1081 ggccggagcg gtccttgttc cgcgccgggt tctcggcggc ccggggattc ttccgcccag
     1141 cgacgagctg accaaggcgg tcatcggcgt cggggggatg ggtctcgggc acatgggtta
     1201 cgagggcgcc cggctcctgg cggtttgcga tgtggacgcc tcccacctcc agaccgccct
     1261 ggacgcggcc gggcccggcg tccgcggcta ccgcgatttc cgtgagatcc tggcccggcc
     1321 ggacatcgac atcgtccata tcgccacccc gccgcactgg cacgccctga tgtcgatcgc
     1381 cgcggtcgag gcgggcaagg atgtctggtg cgaaaagccc atgacccgga cgatcggcga
     1441 ggggatccgg gtcgtcgacg ccgtccgtcg gacggggcgg atgttccgcg tcaatacctg
     1501 gttccgcttc caggaccgat tctatggatt cgggacgccg gtcaagccgc tcaagaaagc
     1561 gctccgcggg ggactcctgg gatggccggt ccgggctgtc ctgaacgcgg ccaccggttt
     1621 cgactggaag ttctactgga gcgggaagac gtaccttccc cccgcgccgg ttccgccgga
     1681 gctggattat gatttttggc tcggccccgc gccgttcaag ccttaccatc cgcaccgcgt
     1741 ccaccagacg ttccgcggct attgggatta cgacggcggg ggactgggcg acatggggat
     1801 gcattacctc gacccggtcc aggatcttct ggataaagac ggaaccagtc cggtcgaaat
     1861 cacggccgac gcgccccagc agcatcccga tgcctgcggc cggtggcgcc gggtctgcct
     1921 gaaatacgcc gacggatgcg aaatcgtcct cgacggcgaa ggccgggagg cggacgcagc
     1981 gtttctgcaa gggcccgggg ggcggatctt ccgcggcctt cattccgaca ttccggattt
     2041 cctggaaaag atcgccgccc ttcccgaccc cgatcccggg gtgacggatt tttccgagtc
     2101 cgtccgaacc cgggtcaagt tcgccctgaa cgaggagaac ggccaccgct cctgcacgct
     2161 ggtcaacctg gccaagatcg ccgtccggct ggggaggccg ctgcgcttcg atccagacgg
     2221 gcagcggttc gtgaatgacg aggaagccga ccgcctgatc gacgaaccgg cgcggtcgcc
     2281 ctggcaccgg gaaggattcg gacggtgaga aagagatgcc gggcgccggc cgccgttttc
     2341 ctgatcgcgt ttttatcctt gaccgcggcc gggcgggatc cggccggtga tctcgccggc
     2401 cgtgtttcgg ctcttctttt gagatttccg gccgattcgg ccgccgaacg cgaggccgcg
     2461 gccgccgagc tgatccggat cggtcccggg gccgtcgccg acctctgcgg ccgcctggcc
     2521 gcccctggaa cggccgatga cagcctggtc cggtatgccc ttgacgcctt agccgtccgg
     2581 gccggacgtc cgggggccga ggccgagcgg aaagcggccg ccgacggaat tctcgccgcg
     2641 ctcacgattc cccgggacca ggaaaacgcg gttttcctga tttcgcttct tcaaaagatc
     2701 ggccggctgg aaatcgtcaa gccgctcggc cggttcttga cccggccgga cctggccgag
     2761 ccggccgtcc gggccctgac ggcgaccggg cttccggaag ccgcggcggc cttggccggg
     2821 gccttggcga cggcccggga cgagacggcc gtcgcgatca tccggggcct gggcgatttg
     2881 cgcgcccgag aagccgggcc ggcgcttctc cgcctggccg aagggaacga cccggatctt
     2941 cggatcgccg ccctggatgc cctggccgat atcggcgacc cggccgcccg ggaggcgctc
     3001 gaacgtgtcc gcgtcgccgc cccgcgggcc gagcgttcgg ccgccgcttc ccggcttctc
     3061 cgtttcggcc ggcgcctggc cgaggaaggc cggccggaca tggccgccga tatcgcccgg
     3121 ccctttcttt cctcatatgc ctcgcccggg gaggagcatc tccgggcggc ggccttgacc
     3181 ctgtttgcgg acgcggcggg ggagagcgag gtacgggacg ccgtccggac ggcgtcccgg
     3241 tcgccggacg ccgcataccg tcggcgggct ctcgacatcg cggccgggcc gcggattccc
     3301 tggtcggccg gcgagtggat cggccttctt gagcgggccg agccggacgc cgcggtcgat
     3361 gtgatccggc ttctggcccg aaagaacgag gcgtcggccc tgcccgcgat ccgggatatc
     3421 ctccgcgcgg gtgaaggcgc cgtccgccgg gaagccgcct tggccgccgc ccgcctgggc
     3481 ggagcggccg tttgggatga gatcgtcccc ttgttcctct ccggaggccc ggccgacgcc
     3541 gccgcggcga aaaaggccgt cctccttttt ccctcggaga gtctcgttcc ggccgtcgcc
     3601 ggctcgattt tttccgctcc cccggccggg cgggtcgctc ttctcgaaat cctcgccgac
     3661 cggagcgccg tctcgacggc cggggccgtt ttctgcctga gctggaaccg ggacgaacgc
     3721 gtccggacgg ccgccctggc ggcccttgag cagaccgtca cggccgagga cttgccggct
     3781 gtcgtcgccc ttcttcttca agcctccacg ccttccgaga ctctcctcct gcagaacgcg
     3841 gccgtcgccg cggcccggcg gatccccgat cccgaagccc gggccggccg gatccttgaa
     3901 tggcttggga ggatcgtcgg gccccggcgg atcgatcttc tccgtccgct ttcccgaatc
     3961 ggcggcgcgg ccgccctgga ggccgtccgg gaggagatcc gaaaggggga ccctcaggcc
     4021 cgggcggtgg ctgttttcac cctggccgcc tggcccgatg aaagcgccgt tccggattta
     4081 ttccggctgg ctcgatccgc gtcggacaag aaaacgcggt atctcgccct ccaaggactc
     4141 gcgcggttga tcccgggatc ttcgtcggcc ccggacgccc gcttggccgc gctttccgaa
     4201 gccttggccg cggccgtcga agccgatgaa aaagccctca tcctggatgc gatcgcggcc
     4261 gcccgcgttc ccgaggggct gggaacgatc cttcgttttc tcgatgatcc ggacctccgg
     4321 agcagggcgg cccgggcggt tttccggttg gtaatgcccg cggccggata cggcggactg
     4381 tcgggcctgg cggcggccgc ggccttgaaa cgagccgttc cttatattga ctacgaattc
     4441 gaccgcgtcc cggccgagcg ccgagccctg gagcttcttg tccaggaggg ctttcagccg
     4501 ctgttcaacg gcaaggatct caccggctgg aagggcctgg cggccgatcc gccggcctgg
     4561 acgaaaatga ccctggagga gatagcccgg gcccaggccg aggcggacgc gtcgatgcgc
     4621 gcccattggc gggtcgtgga cggcgtcctg tcgttcgacg ggcggggcgg caacctctgc
     4681 acggcccggg attacggcga cttcgagatg ttcgtcgatt ggagaatcgg ccccggcggc
     4741 gacagcggcc tctatctgcg cggttctccc caggtccaga tctgggatcc ggatcagcgg
     4801 ccggagggtt cgggcgggct ttacaataac cggatccatc cgcgttcgcc agccgtccgg
     4861 gccgaccggc cggccggcga atggaacacg ttctttattc gaatgacggg ggagagggtg
     4921 acggtcgatc tcaacggcgt ccgggtcgtc gatgatgtcg tgatggagaa ttattgggag
     4981 cgggacaagc cgatttatcc cgcgggacag atcgagctcc aggcccacga cacgccgctt
     5041 gaattcaaga acatcctgct gcgggagatc ccggccggcc ggaccggatc ctgacgggtt
     5101 cggggcgccg gaggaaacgg cggggaattg actaattccg cggcgcggat atgataggat
     5161 caagatggcg gaaggaaagc gccatcgctt acggaggtat ccatgcctga tcccaaatcc
     5221 ccccccgaaa acggcttatc gcgccgcgcc tttcttggaa cggcggcgac ggcggcggcc
     5281 ggcttcatga tcgttccccg gaaagtcctc ggcggccccg gccaaaaagc gccgagcgac
     5341 accctgaaca tcggctgcgt cgccgtcggc ggcaagggct tttccgatgt ccaggacgtc
     5401 agctcggaga acatcgtcgc cctctgcgac gtggacgatc agaagatggc cgccttcctc
     5461 acttccgacg agcatcccta cgacaagaag atgaaatacg agaaggctgc caagtaccgg
     5521 gactggcgga aaatgctcga gacggagaag aacctggacg cgatcaccgt ctcgacgccc
     5581 gaccacaatc acgcggtcat cgccatggcc gccatgaaga tgggcaagca tgtcttcgtc
     5641 cagaagccgc tgacccatac ggtcaaggag gcccggctgc ttgaggagga ggcgaaaaaa
     5701 cgcaacctgg tcacccagat gggcaaccag ggccacgccc gggaaggcgc ccgcctgatc
     5761 tgcgaatgga tttgggacgg ggcgatcggc gatgtgaccg aggtccatgt ctggaccgac
     5821 cggccgatct ggccccaggg cgtcgaggcc ccgaaggaaa tcccgtccgt cccgcccaat
     5881 ctcgattggg acatctggct ggggccggct ccctggcggc cgtaccaccc ggccctgact
     5941 catttcgtct ggcggggttg gtttgattac ggcacgggcg cgctcggcga catgggcgcc
     6001 cacctgatcg acatgccgta ctgggctctc aagctcggcc accccctgac cgtccaggcc
     6061 agctcgaccc ggccgaccaa ggattcgtac ccggtcgccg aaatcgtgac ctacgagttc
     6121 ccggcccgcg gctccatgcc tccggtcaag atggtttggt atgacggcgg actgacgccg
     6181 ccgcggccgg tggtttcggt cctggaaaac ggccggatga tgggcgacga ggacggcggc
     6241 ggcgtgctct tcgtcggcca aaaaggcatg atcatgtgcg gcacgtacgg cgacaacccg
     6301 cgcattcttc cggagtcgct gatgcagtcg tatcagcgcc ccgagaaaac catcccccgg
     6361 tcccccggga tcgcggccga atggatcgag gcgatcaaga agggaacgaa atcgacgacc
     6421 gacatcccga cctattccgg cccgttgacc gaggttatgc tgctcgggaa catcgctatg
     6481 cgcttccggc agtccaagac catcctccag tgggatccgg tcaaaatgga attcccgaac
     6541 ctgcccgagg ccaatgcgtt tgtccacaag gaataccgga tcggctggtc gctgtaagac
     6601 ggacgccaag cggagcatgc ttcgcccgtc cggccggaaa accagcgatg cccggccggg
     6661 cgggattttt tccggggcga ggatcttcag gttgttgatg attttatccg ccttcgtcgt
     6721 tgcggtttcc ggcgggaacc cgccggagga tctccggaca tgggcgattc acgatccggc
     6781 ccggccccgg ccgccggccg tcgaccccgg accggccggt ccttccgtgc cggctccggc
     6841 cggcgcggcc gttcttttcg acgggcggga cctctcccgg tggacggcga tcgggggcgg
     6901 gccggccggg tggaaggtgg aaaacggctt tatggaagtc gtcaagggaa ctggatccat
     6961 ccggacgagg gaggaattcg gggattgccg cctccatgtc gagtgggccg ctcccgcgga
     7021 ggttcgcggt tcgggccagg ggagaggcaa cagcggcgtt ttcctgatgg acaggtatga
     7081 gatccagatc ctggacggct gggaaaatcc gacatacgcc gacgggatgg cggccgcggt
     7141 ctacgggcag tatcctccgc tcgtcgacgc ctgccgccgg ccgggcgagt ggcagaccta
     7201 cgacatcgtt ttccgggcgc cccggttcga tgcttcgggc gggctcctct cgccggcccg
     7261 gatgaccgtc ttccacaacg gaattctcgt tcaggacgac gtccccctga ccggcccgac
     7321 ggctcacaaa gcccggccgc cctatgcggt ccacccggaa aagcagcctc tttccctcca
     7381 ggatcacggc catccggtcc gttttcggaa tatttggatc cggccgcctt cctaagattc
     7441 ttttatctcc gtccggagac aacggcttcg gccggccggt tcctcctgaa aacgcgcgga
     7501 gccgcggccg aacgcttggg ggcgtccgga caaggcccgt ccatcgcctt ttgtccgcgc
     7561 atcgggtaca atcctcaaca tgccaagcga tacgcaagtg atttattcga tgatcggcgt
     7621 cgggcggatt catccgccca accggcaggt cctgaaggac atctccctgg gattttatta
     7681 tggagccaag atcggcgttc tcggcctcaa cggctcgggc aagtcgacgc ttctccggat
     7741 catggccggc ctggatcccg actatatcgg cgaaatcgcc aaaagcaagg gatataccgt
     7801 cggcctgctc gagcaggatc caaagctcga tcccgggaaa accgtcatcg agatcgtaag
     7861 ggagggagtc caaccgatcg tcgacctgct ggcccgctac gacgcggtca acgagaagtt
     7921 cgctgatccc ggggccgaca tggacgccct gatggcccag caagccaggc tcttggagga
     7981 aatcgagaag cacgacgcct ggaacctcga caaccacctc gaaatcgcca tggaggctct
     8041 ccgctgtccg ccgccggaca cgccggtgac gatcctttcc ggcggggagc ggcgccgcgt
     8101 cgccttgacc cgtcttctcc tgatggagcc cgacatcctc ctcctggacg agccgaccaa
     8161 ccacttggac gccgagtccg tggcctggct cgagcgccac ctccaggagt acaagggcac
     8221 ggtcatcgcc gtgactcacg accgatattt cctcgataac gcggccggat ggattctcga
     8281 gctcgaccgc ggccacggga ttccctggaa gggtaattat tcctcgtggc tcgagcagaa
     8341 gaccgcccgg ctggccgtcg aggaaaagac cgaatcccgc cgccggagga ccctggcccg
     8401 ggagctcgag tggatccgca tgtcgcccaa ggcccggcag gccaagggga aagcccgggt
     8461 gacggcgtat gaaaagctcc tcagccggga atacgaccgg tatcgcgacg agcaggaaat
     8521 tccgattccc caggggaacc gcctgggcga catcgtcatc gaagccgagg gagtgaccaa
     8581 agcctatggc gaccggctcc tgatcgatga tttgagcttc cgcgtcccgc ccggctccat
     8641 cgtcggcgtc atcggaccca acggggcggg caagacgacc ctcctccggc tgatcacggg
     8701 gatggagacg cccgacgcgg ggaggatccg gatcggcgaa acggtcaagc tcgtctatgc
     8761 cgatcaatcc cggcggctgg atccggaccg gaccgtgttt gaggcggttt ccggcggggc
     8821 ggagacgatg atcatcggcg gccgggaagt gaacgcccgg gcgtattgcg cctcgtttgg
     8881 gttcgcgggg acggaccagc agaaaaaaac cgcggttctt tcaggcggcg agcggaaccg
     8941 ggtccacttg gcccgggcca tggccgaggg aggaaacgtc ctgctcctgg acgagccgac
     9001 gaacgacctg gacgtcaaca cgctccgggc cctggaggac gccctggaga gcttcgccgg
     9061 gagcgccgtg atcgtcagcc acgaccgatg gttcctcgac cgggtggcca cccatatcct
     9121 ggccttcgag gaggacggga gcgcccggtg gatcgacggc aattataccg cgtatcgcga
     9181 ggatctccgc cggcggaagg gcgccgcggc cgatcagccg caccgcctca agcacaagac
     9241 gctgaagcgt tcgaccggat gaggccggcc tcggggggcc gggccggctt tagatctcct
     9301 cgccgggttc cggcgcgttt tccagattct cctcgtcctg gccgtctatc cgcttttcga
     9361 tgatcttgag ctccttccgg aaccggttga cgacgtattc gatctttttg aggaggtcgt
     9421 acggcttcag cggcttggag aggtaatcgt ccatgccggc ttcgaggcac ttctcccggt
     9481 cgcccttcat ggcgtgggcc gtcatagcga cgatcggcag gtgccggtcg gtgccggcct
     9541 ccgctttccg gatttccgcc gtggcctggt aaccgtccat ctttggcatc tggacgtcca
     9601 tcaaaacgag gtcgaacggc tctttttcca ggaactcgag ggcctcgacg ccgttggccg
     9661 cgaccttgac ctggtgctcg cgccgctcca ggatgcggac ggccagcttc tggttgatga
     9721 cgttgtcctc ggccaggagg atcttgtact gtccgcgggc ccggctcagc gagtgccggg
     9781 tgatgaaggg ggcctgggcg tcgcccggct tgcggacgcc cgccccgagg gcgagcatga
     9841 tcgcgtcgag gaggtacgac tgcttgatgg gcttcgtcag gtaggccgag aggcccagct
     9901 tccggcaccg ggccgcctcg ccgcggaacc cggccgagct cagcatcatg atgaaggcgt
     9961 tgccgagctc ggggtgtttc ttgatttcct cggccagggc gtagccgtcc atctccggca
    10021 tgttcgcgtc gatcaggatc agctgatagc tccgtccgtg ctcccgggcc tcccggaggg
    10081 ctttcaaggc cgttttcccg tcggctacga ccgtcggccg catgtgccag ttggtcagca
    10141 tctcgtggag gatgcggcgg ttggtttcat tgtcgtccac ggccaggacc gacgttccct
    10201 tgatgtcctc gaactggagg gggacgacct cggtctcgcc gccctgctga agggccaggg
    10261 gcatggtgaa gtggaactgg ctgccgatgc caagctcgct ttccacccag atcttgcccc
    10321 ccatcagctc gaccagctgg gagacgatgg acaagccgag cccggttccg ccgtagacgc
    10381 gggtcgtcga gctgtcgact tgggcaaagg gatcgaagat ctgcttgatt ttttccggcg
    10441 ggatcccgat gccgctgtcc ttgacgacga cgtgcagcca catccggtcc tcg
//
LOCUS       DAOOXH010000087        10449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_6868_length_10449_cov_7.595536, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000087 DAOOXH010000000
VERSION     DAOOXH010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 10449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10449
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6868_length_10449_cov_7.595536"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2141
                     /locus_tag="PKV64_09305"
     CDS             <1..2141
                     /locus_tag="PKV64_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HPN17309.1"
                     /translation="IVISLPASDTQGSPHTQADDAEVGGLRGREALERLRNVVGRVES
                     SWRPATAEEGFEIVRRRLFEPLAGPEAFKQRDVTARAFADLYRTQSAEFPPECKGGDY
                     EKRIQAAYPIHPEIFDRLYTDWSTLLKFQRTRGVLRLMAAVIHSLWEKGDRNPLILPS
                     TLPIDDHRVQSELTRYLSDNWAPIIEKDVDGPDSLPLKIDSELPNLGKLHATRRVARA
                     IYLGSAPTAAAAHRGLEDRRVKLGCVMPGESPAVFGDALRRLAASATYLYQDGPRFWY
                     ATQPTVTKIAIDRAEQLKRDPDKVMQELDKRLRLNLRTLGDFSRVHSMPRSSADVPDD
                     LDARLVVLPADHPHIKEPGGLAEAMVREILESRGTTPRLYRNTLVFLAADKVRLQDLD
                     EAVRKFLAWESILAEKEALNLDPHQVRQAETQLQAADGTVTGRLPETYQWLLVPEQIN
                     PQASTAWQAIRLSGGESLAVRASKKLRSEELLVLSLGSTILRKHLDGVPLWRGDHVAV
                     RQLVEDFAKYLYLPRLAGPEVLTQAMRDGISMLTWQSDTFAYAESFDEGAGRYQGLRA
                     GSMAALTADIGLLVKPDIAIRQMEAEIPVPAPAGASSGATGEGGAAGGPGDGGVTGGI
                     GPGGRPAAAPVARRFHGTVELDSARVGRDASRIADEVIAHLAGQVGAEVTVTLEIEAR
                     LTGGASDQLVRTVTENSRTLKFKSHGFEKE"
     gene            complement(2172..2612)
                     /locus_tag="PKV64_09310"
     CDS             complement(2172..2612)
                     /locus_tag="PKV64_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018769396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPN17310.1"
                     /translation="MESLVIYDSNHGNTRKIAETVAEILGAPAVSVSDAGPEHFSGLG
                     LVVVGSPILGWKPSERTQAFLAALKKEQLKGVKAAAFDTRVKLFIHGDAAGKIAKVLA
                     DAGAEMISEPQPFYVKGKEGPLFEGELEKARAWAATLKTNAGPA"
     gene            complement(2683..3108)
                     /locus_tag="PKV64_09315"
     CDS             complement(2683..3108)
                     /locus_tag="PKV64_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17311.1"
                     /translation="MKSQLRRAAAAAAVILALGTLNALAADRYVISFPFKAAGTSFPA
                     GEYVLSLKEDGGLVLRRESTGAETVLPFTERIARSAPPGEEPRLVFHMVGDFKPSYSE
                     YITVYILAEAWPPEVDGFRLHTTKGAHKEIAVKGTVKEP"
     gene            complement(3140..4504)
                     /locus_tag="PKV64_09320"
     CDS             complement(3140..4504)
                     /locus_tag="PKV64_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPN17312.1"
                     /translation="MKSQIWDRRSFLRLCGAVGITAAAVNAGGLERIEAAARSTAGRT
                     PEEIAADEFYWREVQLAYKLDRTLINLNNGFTAPCPRVVLESEFRYLEMINMLPVHYQ
                     AMVAENVETIRRRMAAEFGCATDELALTRGASESLQIVQNGLDLEAGDEVITTEQDYP
                     RMLTTWDQRMRREGIKVTRLQFPVPATQDQLYRLFEEAITPRTKVFHFCHITNLTAQL
                     FPVQRLARLARSRGIVTIVDGAHALGQFPFKLRDLECDAYGVSLHKWLMAPIGNGCLY
                     VRREMIPKFWPLQAAPEQQDGDIRKFEAIGTHPWGIRAALGEALAFHQAVGGERKAAR
                     LRYLTLRWAAALKAHPRVRILTNLGEPPEAWGVAAVNVEGLDMRNLAAFLKSKYRIVA
                     VPLVGGAPPNQVFDYQALRVSPNIYTTLEEIDTFIEGMEDAIKNGIPPEGGTAPAFPM
                     ESAG"
     gene            4763..6157
                     /locus_tag="PKV64_09325"
     CDS             4763..6157
                     /locus_tag="PKV64_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HPN17313.1"
                     /translation="MFEPRIYKERRKRLLRAVGSGLGLFLGNEESPMNYPDNTFRFRQ
                     DSTFLYYFGLDRPGLAAVMDFDEGTATVCGDDYTVDDIVWRGPQPAIREMSRRAGVDR
                     TGTADELAGRLKDARRAGRRIHFLPPYRAENALKLKRWLGLNPDRAAAAASVDLIRAV
                     VEQRAVKGPEEIAELDRAADISTDMHVAAMRMVRPGVAEAEIAAEVHRVALAAGGDIS
                     FPIIASVRGETLHNHDHGNRLKRGDLFLLDAGAETALHYAGDLSSTVPASGTFTGRQR
                     EIYDITLAAHEAAAGALRPGVNHLDVHLLACRTIAWGLKEAGLMKGDPEDAVQAGAHA
                     LFFPCGVGHMMGLDVHDMEDLGEVYVGYEGRPKYTQFGLKSLRLARPHKPGFVVTIEP
                     GIYFIPQLIDQWRAEGKFTDFIVYDKVEKYRTFGGIRNEEDFVITPRGSRRLGKAKPK
                     TTAEVEAVLAAARK"
     gene            complement(6182..7225)
                     /locus_tag="PKV64_09330"
     CDS             complement(6182..7225)
                     /locus_tag="PKV64_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPN17314.1"
                     /translation="MPQTEKTSTLAAAAPDTRILVAGAGGFIGGSLVRYFKDKGFANI
                     RAVDKKPLSDWYRKTPGVECLCLDLSHEKNCRRAVEGMDEVYQLAADMGGMGFIERFR
                     IECLRSVLINAHMIESAYRAGAARYFFSSSACVYNTDLQKNPKITALKESDAYPAMAE
                     RGYGWEKLISEMFCQEYWAERGFKTFIARFHNVYGPWGTWDGGREKAPAALCRKIIKA
                     IDDGTNEIKIWGDGTRTRSYQYIDDCVTGIDKITHCEELIATPINLGTSELVSVNELV
                     SIIEKIAGVAVKRTYDLTAPQGVGGRNSDNTFIKKILGWEPDIPLRAGLAKTYAWIKE
                     QYERKKAGLKTVD"
     gene            complement(7259..7996)
                     /locus_tag="PKV64_09335"
     CDS             complement(7259..7996)
                     /locus_tag="PKV64_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPN17315.1"
                     /translation="MKPLFETLRSIFLWTLAFPVFVLSCLAILLTALFTRGRALEPVI
                     KTGCRVVLFCAGVRVRLHGRENFDPARQYLVMMNHVSLLDPLVFYARFPGRARTVEEE
                     RHFRWPLYGPMLRRIGQIPLDRSHPRKARESLARAAEFIRTRKDFSFIVFPEGTRTLD
                     GNLGPFKRGGFLLAAEAGLDILPIVQTGAYRIMRKGSKRLRPGRIDCYLEPAVAADGS
                     SRRDIAGWMDRVRRVIEQRLDGPGILA"
     gene            complement(7993..8907)
                     /locus_tag="PKV64_09340"
     CDS             complement(7993..8907)
                     /locus_tag="PKV64_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HPN17316.1"
                     /translation="MEKKDRTLLVVNPVSGHGRGRKVFSRMEPALREAFPGLEVRVSE
                     FPGQAFQFGREAVRDRYGRVLCLGGDGTPFEVVNGMYLQGRPPFLPEIGIIPSGTGNS
                     FLLEFGIQTPEEALRRIAAGRRVKVDLLEFDYQRNGRPERRYSLNLIGVGLITDILKL
                     TNDWLKFLHAFGYSLAVLIRLARGMHNTIDLEVDGRKWTVPDSALVVSNSKYTGGKMK
                     IAPHAETSDGKADLVLFRNVDRREMIQIFKGVFSGAHAAHPKVDIIQGVNMTVSGTPD
                     LHVMADGEVLGRTPLRIKLLPGELTFLG"
     gene            9105..9650
                     /locus_tag="PKV64_09345"
     CDS             9105..9650
                     /locus_tag="PKV64_09345"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPN17317.1"
                     /translation="MIRGRECPGGCPARIRRAGGFTLLETLVTLAIIGILTVIFYPSV
                     KNSFEMRSFDNASRDILTTLQVAKWQAVNTKYFHRVRFAGDSAGWTYRIEIEKPYGTW
                     TLKQGQQVKRIPSEFGVTLTLPADLSIVFDGTGFVSGFDTAKNQISLSSSRLALLGEP
                     HYRTIRFYASGSTQFIKGSTG"
     gene            9654..10112
                     /locus_tag="PKV64_09350"
     CDS             9654..10112
                     /locus_tag="PKV64_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPN17318.1"
                     /translation="MLKLVRRRKTTGGEKGFSLIEALIGISIIGIAMLGLGQAFLLSV
                     NNNRRAGEISHATFLAQQQLDYLRTLTAAELSVFPSTAQGESSDQALDPNGDGYVDFR
                     RITQITTGGTGYAVKILVFPATAAGKTSAALLAAPFSYNVRAILHSIIIR"
     gene            10121..>10449
                     /locus_tag="PKV64_09355"
     CDS             10121..>10449
                     /locus_tag="PKV64_09355"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPN17319.1"
                     /translation="MKKHRSAFSRRVRGFTLIELLIGSAIMLVVVVAALSVYSRSNKI
                     SADQQQFIEMQTDVRAAMYFVSRDARMSGTGLTEALAGYAVEGVDNETTGTTETPDRL
                     KILGNLE"
BASE COUNT         1872 a         3247 c         3383 g         1947 t
ORIGIN      
        1 tgattgtgat ttcgctgccg gcctccgaca cccagggctc gccgcatacg caggctgatg
       61 acgcggaagt gggcggtctg cgcggccgcg aagcgctcga gcggctgcgc aatgtggtcg
      121 gccgggtgga atcgtcttgg cggccggcga ccgccgagga gggcttcgag atcgtccggc
      181 gccggctgtt tgagccgttg gccgggccgg aagccttcaa gcagagggat gtgaccgcgc
      241 gggctttcgc tgatctctat cgtacccaaa gcgccgagtt cccgccggag tgcaagggcg
      301 gcgactacga aaaacgcatc caggcggctt atccgatcca cccggaaatc ttcgaccggc
      361 tctacaccga ctggtccacg ctgctcaagt ttcagcgcac acgcggcgtc ctgcgcctga
      421 tggcggcggt gatccacagc ttgtgggaaa agggagaccg caatcccctg atcctgccgt
      481 ccacgttgcc gatcgacgac catcgcgtcc aatccgagct gacccgctat ctttcagaca
      541 actgggcgcc gatcatcgaa aaagatgtcg acgggccgga ctcgttgccg ctcaagatcg
      601 atagcgagct gcccaacctc ggtaagctgc atgcgacgcg aagggtggcc cgggccatct
      661 acctcggttc cgccccgacg gccgcggccg cgcaccgcgg cctcgaggac cggcgggtca
      721 agctcggctg cgtgatgccg ggcgagtcgc cggccgtctt cggcgacgcg ttgcggcgtc
      781 ttgccgcgtc ggcgacctac ctctatcagg acgggccgcg cttctggtat gccacgcagc
      841 cgacggtcac caaaatcgcc atagaccggg ccgaacagct caagcgagat cccgacaaag
      901 tgatgcagga attggataaa cggcttcgcc tgaatttgcg cacgctcggt gatttttcgc
      961 gcgttcactc catgccgcgt tcaagcgcgg acgtgcctga cgacctcgac gcccggttgg
     1021 tggttttacc cgcggatcat ccgcacatca aggaaccggg cggcttggcc gaagccatgg
     1081 tccgggagat tctcgaatcg cggggcacga cgccccgcct ctatcgcaac accctggttt
     1141 tcctcgcggc tgacaaggtg cgcctgcagg accttgacga ggcggtgcga aaattcctgg
     1201 cgtgggagtc gatcctggcc gagaaagagg cgctcaacct cgatccgcat caggtgcgcc
     1261 aggccgaaac gcagttgcag gcggccgatg gaacggtgac gggccgattg cccgaaacgt
     1321 accaatggtt gctggtcccg gagcagataa atccccaagc gtcgaccgcc tggcaggcga
     1381 tccgtctttc gggaggcgaa tcgctcgctg tgagggcgag taagaagctc cgcagcgaag
     1441 aattgctggt gctgtcgctc ggctcgacga tcttgcgcaa gcacctcgac ggagttcctt
     1501 tatggcgagg cgatcatgtc gccgtaaggc agctcgtgga ggattttgcc aagtatctct
     1561 acctgccgcg tttggccggt cccgaggtgc ttacgcaggc tatgcgcgac gggatttcca
     1621 tgctcacgtg gcagtcggat acgtttgctt atgcggaaag ttttgatgaa ggcgccggcc
     1681 gctatcaagg tttacgcgcc gggtcgatgg cagccctcac ggctgatatc ggcttgttgg
     1741 tcaagccgga tattgcgata cgacagatgg aagccgagat ccccgttccg gctcccgcgg
     1801 gtgcttccag cggagcgacc ggtgaaggcg gggccgcggg cggaccgggg gatggcggcg
     1861 tcaccggcgg cataggcccc ggcggccggc cggctgccgc tccggttgcc agacgtttcc
     1921 atggcaccgt cgagctcgat tccgcgcgcg tcggccgcga cgccagccgc atcgccgacg
     1981 aggtgatcgc tcatctggcc ggtcaggtcg gcgcggaggt aacggtcacc cttgaaatcg
     2041 aggccagact caccgggggc gcctccgatc aactcgtccg gacggtcacc gaaaacagtc
     2101 gtacgcttaa gttcaagagt catgggtttg agaaagagta gtatacagcg ttataacgac
     2161 tgaaaggcca ttcaagccgg gccggcgttt gtcttcagag tcgcggccca agcccgggct
     2221 ttttccaatt cgccctcgaa aagcgggcct tcttttcctt tgacgtaaaa gggctgcggt
     2281 tccgaaatca tctccgctcc ggcgtcggcc aagaccttgg cgattttgcc ggccgcgtcc
     2341 ccgtggatga acaatttgac ccgggtgtcg aaggcggcgg ctttaacgcc cttgagctgt
     2401 tcctttttca aggcggcaag aaaggcctgc gttctctccg acggcttcca gccgagaatc
     2461 gggctgccga caacgaccag cccgaggccg gaaaaatgct ccggcccggc gtcggaaacc
     2521 gaaacggccg gcgcgcccag gatttccgcg accgtttccg cgatttttcg ggtgttcccg
     2581 tgattcgagt cgtaaatgac gagagattcc atgaatttaa tcctccaggg gtgcagtctg
     2641 tatcctcaaa aatttcggaa taatattctg tgaagcgggc cgctacggct ccttgaccgt
     2701 tcccttgacg gcgatttcct tgtgggctcc cttggtcgtg tggaggcgga aaccgtccac
     2761 ctccggcggc caggcttcgg ccaggatata gaccgtgatg tattccgaat aggacggctt
     2821 gaaatcgccg accatgtgga atacgagccg gggctcctcg cccggcggag cggaccgggc
     2881 gatccgttcg gtgaatggaa ggacggtttc ggctcccgtc gattcccggc gcaggacgag
     2941 gcctccgtcc tccttcaggg agaggacgta ttcccccgcc ggaaagcttg ttccggcggc
     3001 cttgaaggga aacgaaatga cgtaccggtc ggcggccagg gcgttcagcg ttccgagagc
     3061 caggatgacg gcggccgcgg ccgccgcgcg gcggagttga gatttcatga gcgttctccc
     3121 tttgccgggc gcgcgggcgt cagccggcgg actccatggg gaaggccggg gccgtccccc
     3181 cctcgggcgg gatcccgttc ttgatcgcgt cctccatgcc ctcaatgaac gtatcgatct
     3241 cctcgagcgt cgtgtagatg ttcggcgaga cgcggagagc ctggtagtcg aacacttggt
     3301 tgggcggggc gccgccgacc agcggaacgg cgacgatccg gtactttgat ttcaggaagg
     3361 cggcgagatt ccgcatgtcg aggccctcga cgttgaccgc ggccacgccc caggcttcgg
     3421 gcggttcccc gagattcgtc aggatacgga cgcgcggatg ggctttgagg gcggcggccc
     3481 agcggagagt caagtagcgc agccgggcgg ccttgcgctc gccgccgacg gcttggtgga
     3541 aggctagggc ttcgccgagg gcggcccgga tgccccaggg atgggtcccg atggcttcga
     3601 atttgcggat gtcgccgtcc tgctgttcag gcgcggcctg cagcggccaa aatttcggga
     3661 tcatctcccg gcggacgtaa aggcagccgt tgccgatggg ggccatcagc cacttgtgaa
     3721 ggctgacgcc gtaggcgtcg cactccagat ccctcagctt gaaggggaat tgccccaagg
     3781 cgtgggctcc gtcgacgatc gtcacgatcc cccgcgaccg ggccaagcgg gccagccgct
     3841 ggacggggaa aagctgggcc gtcaggttgg tgatgtggca gaaatggaag accttcgtcc
     3901 ggggggtgat cgcttcctcg aacagccggt agagctggtc ctgcgtcgcc gggacgggaa
     3961 actgcagccg ggtgaccttg atcccctcgc gccgcatccg ctgatcccag gtcgtcagca
     4021 tccgggggta atcctgctcg gtcgtgatca cctcgtcgcc ggcttcgagg tcgaggccgt
     4081 tctggacgat ctgcagggat tcgctggctc cgcgggtcag ggccagctcg tccgtggcgc
     4141 agccgaattc cgcggccatc cgccgacgga tcgtctcgac gttctcggcg accatcgcct
     4201 ggtaatggac ggggagcatg ttgatcattt ccaggtagcg gaactcggat tcgaggacca
     4261 cgcggggaca gggagcggtg aagccgttgt tcagattgat cagggtccgg tccagcttgt
     4321 aggccagttg gacttccctc cagtagaatt catcggcggc gatctcctcg ggcgtccggc
     4381 cggcggtcga ccgggccgcg gcttcgatcc tttcgagccc gccggcgttg accgcggcgg
     4441 cggttatccc cacggctccg cacagacgca aaaacgaccg ccggtcccag atttgagatt
     4501 tcatgaaacg gctcctcctt tgcgggatga cttattatat tcgcaattcc cattctccgg
     4561 tcaagcggaa aaccctttga tttccggcgc cttcatgaga gaatacgcgc ccggcggaag
     4621 ccggacatcc gtccgcggcg gcgaggccgc ctcgagactt gcggcgccgg gctccctgac
     4681 aaggtcagac aatcctcccg ggccgggagg acggaggtcc ctggttttat cggccggcga
     4741 aaatgctgaa ggaggttctt tgatgttcga accgcggatt tataaagaac gccgaaaacg
     4801 gctgctccgg gcggtcggat cgggcctggg cttgttcctg ggcaacgagg aaagcccgat
     4861 gaattatccc gataacacgt ttcgcttccg tcaagacagc acgtttctct attatttcgg
     4921 gctggaccgg cccggcctgg cggcggtgat ggacttcgat gaggggacgg cgaccgtctg
     4981 cggcgacgac tatacggtcg atgatattgt ttggcgaggc cctcagccgg ccatccggga
     5041 gatgagccgc cgggccggcg tcgatcggac cgggacggcg gacgagctgg ccgggcggct
     5101 gaaggacgcc cgccgggccg ggcgccggat tcatttcctg ccgccgtacc gggccgaaaa
     5161 cgccctcaag ctgaaacgat ggctcggcct gaacccggac cgggccgcgg ccgcggcttc
     5221 ggtcgatttg atccgggcgg tcgtcgagca acgggcggtc aaggggcccg aggaaatcgc
     5281 cgagctcgac cgggcggcgg acatctccac ggacatgcac gtggcggcga tgcggatggt
     5341 ccggccgggg gtcgccgaag cggagatcgc ggccgaggtc caccgcgtcg ccctcgccgc
     5401 cgggggcgac atctctttcc ccatcatcgc ctccgttcgc ggggaaacgc ttcacaacca
     5461 tgaccacggc aaccggttga aaaggggcga cttgtttctc ctcgacgccg gagccgagac
     5521 ggctcttcat tacgccgggg atctgtccag caccgtcccg gcctccggga cgttcaccgg
     5581 ccgccagcgc gagatctacg acatcaccct ggccgcccat gaagcggccg ccggagcgct
     5641 tcgtccgggc gtcaaccacc tcgatgtcca cctcttggcg tgccggacca tcgcctgggg
     5701 tctcaaggag gcgggcttga tgaagggcga cccggaagac gccgtccagg ccggggccca
     5761 cgccttgttt tttccctgcg gcgtcggcca catgatgggc ctcgacgtcc acgacatgga
     5821 ggacttgggc gaggtctatg tcggctacga aggccgtccg aagtacaccc agttcgggct
     5881 gaaatccctc cggctggccc ggccgcacaa gccgggcttc gtcgtgacca tcgagccggg
     5941 gatttatttc atcccgcagc tgatcgacca gtggcgggcc gagggcaagt tcaccgattt
     6001 catcgtttac gacaaggtcg agaaataccg gacgttcggc ggcatccgca acgaggagga
     6061 tttcgtcatc actccccggg gaagccggcg tttgggcaaa gccaagccca aaacgacggc
     6121 cgaagtcgag gcggtcctgg ccgcggcgag gaaatgaggc cggcggccgt cctcccgccg
     6181 gtcaatcgac ggttttcaaa ccggctttct tccgctcgta ttgttccttg atccaggcgt
     6241 aggtcttggc cagtccggcc cgcagcggga tatccggctc ccaaccgagg attttcttga
     6301 tgaacgtgtt gtcggagttc cggccgccga cgccttgggg cgcggttagg tcataggtcc
     6361 gcttcacggc gacaccggcg attttttcga tgatcgagac gagttcgttg acgctgacga
     6421 gttccgaggt gcccaggttg atgggcgtgg cgatcagctc ctcgcagtgg gtgatcttgt
     6481 cgatccccgt gacacagtcg tcgatgtact ggtagctccg ggtccgggtg ccgtcgcccc
     6541 aaatcttgat ctcgttcgtt ccgtcatcga tcgccttgat gatcttccgg cagagagccg
     6601 ccggggcctt ttcccgcccg ccgtcccacg tcccccacgg gccgtagacg ttgtggaacc
     6661 gggcgatgaa cgtcttgaat ccgcgctcgg cccaatactc ctggcagaac atctcggaga
     6721 tgagcttttc ccagccgtag ccgcgctcgg ccatcgccgg ataggcgtcg gattccttga
     6781 gggccgtgat cttgggattc ttctggagat cggtgttgta gacgcaagcc gaggaggaaa
     6841 agaagtaccg ggccgcgccg gcccgatagg ccgattcgat catgtgggcg ttgatgagaa
     6901 cggaccgcag gcactcgatc cggaaccgct cgataaatcc cattccgccc atgtcggcgg
     6961 ccagctggta gacctcgtcc atcccctcga ccgcccggcg gcagttcttc tcgtggctca
     7021 ggtcgaggca aaggcactcc accccgggcg ttttccggta ccagtcggac agcggctttt
     7081 tgtccacggc tcggatgttc gcaaagccct tgtccttgaa ataacggacc agcgatccgc
     7141 cgatgaaacc gccggcgccg gcgacgagga tgcgggtgtc gggggccgcg gcggcaagcg
     7201 ttgaagtctt ttccgtctgg ggcatgatcg acctccaaag gatcgaattg cccccaattt
     7261 atgccaggat ccccggtccg tcaagccttt gctcgatgac gcggcggacc cggtccatcc
     7321 agccggcgat gtcccggcgc gaggagccgt cggcggcgac ggccggctcg aggtagcaat
     7381 cgatgcgccc gggccgcagc cgcttgctcc ccttgcgcat gatccggtac gctcccgttt
     7441 gaacgatcgg caggatgtcc aggcccgctt ccgcggccag gagaaatccg ccgcgcttga
     7501 agggcccgag gttgccgtcc agcgtccgcg tgccctcggg aaagacaatg aaggaaaaat
     7561 ccttccgggt ccggatgaat tcggccgccc gggccaggct ttcccgtgct ttccggggat
     7621 gggagcggtc gagcgggatc tggccgatcc gccggagcat cgggccgtag agaggccagc
     7681 ggaaatgccg ttcctcctcg acagtccggg cccggcccgg aaaccgggcg tagaaaacca
     7741 gcggatccag gaggctgacg tggttcatca tcacgaggta ttgccgggcc gggtcgaagt
     7801 tttcccgccc gtggaggcgg acccggacgc cggcgcaaaa gaggacgacc cggcagccgg
     7861 ttttaatgac gggttccagg gcgcggcccc gggtgaacag ggccgtcaga agaatcgcca
     7921 ggcagctgag gacgaacacg ggaaaagcga gggtccagag aaaaatggag cggagcgttt
     7981 cgaacaacgg cttcatccga gaaaggtcaa ctccccggga agcagcttga ttcggagcgg
     8041 cgtccgtccg aggacctcgc cgtcggccat cacgtggaga tccggcgtcc cggagaccgt
     8101 catgttcacg ccctggatga tgtccacttt gggatgggcg gcgtgcgctc cggaaaaaac
     8161 gcccttgaag atttggatca tttccctccg gtcgacgttt cggaagagca ccaagtcggc
     8221 tttcccatcc gacgtttcgg cgtgcggggc gattttcatc ttcccgccgg tgtacttgga
     8281 gttggaaacg accagggcgc tgtcgggcac ggtccatttc cggccgtcga cttcgaggtc
     8341 gatcgtgttg tgcatgcctc tggccagacg aatcaggacg gccaggctat agccgaaggc
     8401 gtgaagaaac ttcagccagt cgttggtcag cttcaggatg tcggtgatca acccgacgcc
     8461 gatcaggttg agggaatacc gccgttccgg ccggccgttc cgctggtaat cgaattccag
     8521 gaggtcgacc ttgacccggc ggccggcggc gatgcggcgg agggcttcct cgggggtttg
     8581 aatcccgaat tccaggagaa atgaattccc ggtccccgag gggatgatcc cgatctcggg
     8641 aaggaacggc gggcggccct ggagatacat gccgttgacg acttcgaacg gcgttccatc
     8701 gccgccgagg cagagaaccc ggccgtagcg atcgcgcacg gcttcgcggc cgaattggaa
     8761 ggcctggccc gggaattccg agacgcggac ctcgagtccc ggaaacgcct cgcggagggc
     8821 gggctccatt cggctgaaga cttttcgtcc ccgcccatgg ccggaaaccg ggttgacgac
     8881 aagcagggtc cggtcctttt tttccacgga accgcttata ccatgcctcc gtttcctttt
     8941 caaacgggga gggcgcggcc gaggggcggg aaccggctag ccgccccccg gccgccatat
     9001 caccccccat ctcaccggat ctgatgattg tcagaatcgg cgccgatgga ctatagtaac
     9061 aatcggagaa ttgccgaaaa aggatatccg agggctttat ttacatgatt cggggaagag
     9121 aatgtcctgg gggctgtccc gcccggattc ggcgggctgg aggattcacc ctgctggaga
     9181 ccctggtcac cctggcgatc atcggcatct tgaccgtcat tttttacccc agcgtcaaga
     9241 attcgttcga gatgcggagc ttcgacaacg cctcccgcga tatcctaaca actctccagg
     9301 tggccaagtg gcaggccgtc aacaccaagt atttccaccg ggtccgtttt gccggcgatt
     9361 cggccggctg gacgtaccgg atcgagatcg agaagcccta tgggacatgg accctgaaac
     9421 aggggcagca ggtgaagcgc attccctcgg agttcggcgt caccctgacc cttccggccg
     9481 atctttccat tgttttcgac gggaccggat tcgtctccgg cttcgacacc gccaagaatc
     9541 agatctccct ttcctcctcg cggctggcgc tcctgggcga gccccattac cgcaccatcc
     9601 gcttctatgc cagcgggtcg acgcaattca tcaagggatc cacggggtga ctcatgctta
     9661 aactcgtccg gaggcggaaa acaacggggg gtgaaaaagg cttttccctg atcgaggccc
     9721 tgatcgggat ctcgatcatc ggaatcgcca tgctcggcct gggccaggcc tttcttctca
     9781 gcgtcaacaa caaccggagg gcgggggaga tcagccacgc cacgttcctc gcccagcagc
     9841 agctcgacta tcttcgaacc ttgaccgcgg ccgaactgag cgtgttcccc tcgaccgccc
     9901 agggggaatc ctccgaccaa gctctcgatc cgaacggcga cggctacgtc gatttccgaa
     9961 gaatcacgca gatcacgacc ggcgggacgg gatacgcggt taagatactc gtcttccccg
    10021 cgacggccgc cggcaagaca tccgcggcct tgctggccgc tccgttttcc tacaacgtcc
    10081 gggccatcct ccattcaatc atcatccggt gagcaccgcc atgaaaaaac atcgctccgc
    10141 tttttcgcga cgcgtccggg gattcaccct gatcgagctt ttgatcggca gcgccatcat
    10201 gctcgtcgtc gtcgtggccg ccctgtcggt ctactcccgg agcaacaaga tttcggccga
    10261 tcagcagcaa ttcatcgaga tgcagaccga cgttcgggcg gccatgtatt ttgtctcccg
    10321 ggacgcccgg atgagcggga ccggcctgac cgaggctctg gccggctacg ccgtggaggg
    10381 ggtggacaac gagacgacgg ggacgacgga gacgcccgac cggctgaaaa tcctgggaaa
    10441 tctcgaaaa
//
LOCUS       DAOOXH010000088        10398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_6914_length_10398_cov_7.797448, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000088 DAOOXH010000000
VERSION     DAOOXH010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 10398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10398
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6914_length_10398_cov_7.797448"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1782)
                     /locus_tag="PKV64_09360"
     CDS             complement(<1..1782)
                     /locus_tag="PKV64_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17320.1"
                     /translation="MRFFKRPLWTCVLASFLSAAVGSFAGPPPAAGRLKLDVRPTVFF
                     VEEAGALLQKCEAVLEYAGPEAPGELRIRGFAAEIRQALPKIGPGRHSVVVHLPDHAR
                     PVRARFSVRVGNSTASRSLVLAPQRKWTVYLWPHSHTDLGYTDFPSRAAKNQAEYLDR
                     VIEFCRATEAYPDEAKFRWNIEIAWALENYIKSRPAEKVQALAGLLRSGRVELSGWYV
                     QLSDLFGHEELVRAVLPAKDFSRTLAFPLGAAMNNDVTGFSWASPQILSQAGIHYFAV
                     GINETRARAPLRKPNPFYWQSPDGAKVLVWNGEHYLFSNLELRLHQGNAESEPRLAEY
                     LSGLQARGDYPYDLIAFPISGRPVDNSPPKRELSDRVAEWNAKWAYPRLRLATMSEFF
                     RVLEKRYGATLPAHKKAWPDYWTDGAGSTAYETGINRLAHDDLTTAEKLAAAASLVES
                     GAPFPKAEIRDGYAQAMLFDEHTWGSQDSISEPEAESVRGQWIQKSSHAYEAREIAGS
                     VRDRSLAVLARGITTGDGLPLAVFNPLSWSRTDVVRVILPRLLAELEGRFRLIDNRTG
                     AEIPFQILDPNILMFRAENVPSIGYAVF"
     gene            1895..2959
                     /locus_tag="PKV64_09365"
     CDS             1895..2959
                     /locus_tag="PKV64_09365"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HPN17321.1"
                     /translation="MSVIFRRAAAAGLGLAVLAAGLLPAGASMPSLSWTIQTSGTSES
                     LRGLSAVDERTAWVSGSKGTVLRTVDGGETWTAVPVPGAEEADFRDIEAFGPEEAVVM
                     AAGRPARLFRTADGGRTWTETYFDDSPGIFLNALAFFDGMNGLALGDPMDGRFFLLRT
                     SDGGRTWRSLPLESRPAALEGEAAFAASGTALHVRGNAAWFCTGGAASRIWLSSDGGV
                     RWEAVSAPLGGGTPSSGGFSVYFLNEKAGIAVGGDYRNEPADADNAAVTFDGGKTWAP
                     VSGRRPGGFREAVAAVPEDPSSPAVTVGPSGSDVSFDGGQTWIAIGGPEGFHTLGFAG
                     NGLAAWAAGRNGLIARLAFQ"
     gene            complement(3241..4965)
                     /locus_tag="PKV64_09370"
     CDS             complement(3241..4965)
                     /locus_tag="PKV64_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPN17322.1"
                     /translation="MEHKNELTIPAILAKSAELYENRNALGFYDSPALSYRQLKDKTD
                     QIAAFLADRGIVHGDRVAILSENSPHWGVAYLAVTSMGAVAVPILTDFSPTEIHHVLR
                     HSEAKAVFVSEKLYGKIDEASFDQLKTRILIDDFSVIKPDTSKDKLRRLIAEGSHELR
                     KIKAMALKWLGRIPSQVQSEDLASIIYTSGTTGHSKGVMLTHGNLVSDVLATTRLVDV
                     NCEDRLLSLLPLAHVYECTLGLITSLMKGASVSYVHKPPTAAVLLPALRAVQPTFVLS
                     VPLIIEKMVRAKVFPALRGKALIRMACAVPALRKMVIRKAGAKIRAAFGGKLNMFCIG
                     GAALAPDVEDFLIEAGFPYCIGYGLTETAPLVTGTKPFETRPRSAGRPLEGVEIRIAD
                     PDPKTGEGEIQLRGPMIMKGYYKDPEKTAEAFSDGWLKTGDLGCLDADGFLYIKGRSK
                     NVIIGPSGENIYPEVIESVINRTDSVQESLVFEEQGRLVARIHLNYEALDAFFSEKKW
                     SASQIREHIRRLLEDIKAKVNEGVASFSRLSRVIEQTEPFEKTPTQKIKRYLYVERTI
                     DGEGAISV"
     gene            complement(5175..5795)
                     /locus_tag="PKV64_09375"
     CDS             complement(5175..5795)
                     /locus_tag="PKV64_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17323.1"
                     /translation="MKTPTFLRPAFGAAAVALFIQSIAVPAAAPAAAVVLTGLLDSER
                     VLEALPGAARAAREYEPHFRAIDGLRGFANPARIEVFLGTWDENGQELIAALLKTLEE
                     AFNPAIEVVWIGVSRDLREPPEARRAGVERVPTIIVQVEGKEKGRIVAVPDNTLEEDL
                     ATILLGVPFSEADFDLFRSRPHSHLPIDCTPCHLPVPAKAGRPAAF"
     gene            complement(5858..6583)
                     /locus_tag="PKV64_09380"
     CDS             complement(5858..6583)
                     /locus_tag="PKV64_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17324.1"
                     /translation="MTPGLTALVSTAATLGVLHTAAGPDHYLPFIVLAKARKWSMAKT
                     LAVTALCGLGHVGSSILIGAVGILFGLGVAKLEIFEGVRGSVAAGLFILFGLGYFLWG
                     LARGLRNRGHHHLHVHTDGTVHGDHSSAEGTDGVRAGHVHAEPKPVNVTPWILFTIFV
                     FGPCEPLIPLLMFPAARESTGGVVLVAAVFSLATIATMAMMVLLPAAGLRLLPMRFLE
                     RYLHAVAGFTILACGLGMAFLGL"
     gene            complement(6662..7774)
                     /gene="menC"
                     /locus_tag="PKV64_09385"
     CDS             complement(6662..7774)
                     /gene="menC"
                     /locus_tag="PKV64_09385"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HPN17325.1"
                     /translation="MKIERLELFLLRLPYVHFFETSFGRSYDRTFILIKVEAEGLAGW
                     GECTAEESPLYSGETTETAWHVLKDFLVPLVFKKNISGPGEFQRAASIYRGNRMAKAG
                     LELAFWDLQAKAAGVPLAKLYGGTRTEIEAGVSVGIEDSLPELVDRVGSYLGQGYRRI
                     KIKIKPGWDAAACRAVRERHPGILLQVDANGAYGPGDASALAALDEFRLLLVEQPYAP
                     YDLWEHAKLQKKMSTPLCLDESILSLATARAALEMGACRIINIKVGRVGGVVEARAIH
                     DHCRSAGVPVWCGGMLESGIGRAHNLHLASLPNFSLPHDLSASRRYYREDIIDPFIEL
                     SGPGTIRVPEGPGIGVNPVPARIARATVRREEFRPG"
     gene            complement(7771..8658)
                     /locus_tag="PKV64_09390"
     CDS             complement(7771..8658)
                     /locus_tag="PKV64_09390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN17326.1"
                     /translation="MKKSPPPKKSISGPDVRIRPLTSLEEFRDCVFIQRRVWDHPDLD
                     LIPDHFFQIAFHTGAILLGAFVDGRLSGYVYSFPADFGRTRGHHSHHLAVLPEVQGLG
                     LGKALKWAQRDEALKRRLELVTWTFDPLQARNANLNIHVLGVVGRTYLRNFYGETPTL
                     GVEEGVPSDRFLVEWRIKSARVRARRQGPPAPVDPRRIPKALEREEGGLEPAIVPGRP
                     NLRLQVPRILVEIPGRIADLPQGEGLVAAWQTAARKAFEAYFARGYRLDDFISGERSY
                     YVLRLTAGTERAPFFGRPS"
     gene            complement(8615..9724)
                     /locus_tag="PKV64_09395"
     CDS             complement(8615..9724)
                     /locus_tag="PKV64_09395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPN17327.1"
                     /translation="MSLFQIDAGRDWRGGQRQAFLLTRELAGRGYPVLLVAQPESPLE
                     KKAAEAGLRVLPVEIKNDAHIQAGWKIGRAMRRESCLLAHFHDGHAAGVGGRAARLAR
                     VPVRIVTRRVEYPLKANRFSRNKYAEADAVIAISQRVRDVLLEGGVPAGRIEVIPSGI
                     DLEPFAGSLDRDYLRREFGFPPDSFLVGIVAQLVDGKGHGTLIEAAKLLKTFLPKIKI
                     IIIGSGALELRLAEQARGLGVGDLVFFLGFRDDVPRLLASLDAFVLTSDAEGLGSSIM
                     DAMAARLPVVATRVGGIPEVVEDGETGILIPPRDPETLARTILRLANDRDLAGRLGAQ
                     GFESVRRKFSAEAMVSRIVDLYEKIAASQKINLRA"
     gene            complement(9705..>10398)
                     /locus_tag="PKV64_09400"
     CDS             complement(9705..>10398)
                     /locus_tag="PKV64_09400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPN17328.1"
                     /translation="SEIVVVDSDSTDLTVKIARRFTTRIFQRKWTNYSDQKNYADGLA
                     SHPWILSLDADERLSPELREEIKALGPGDPDCSGFSVPRRVYYLGRWIKHSGWYPDRK
                     VRLFRKDRARWEGAYVHEDLVVEGEIRKLHGDLLHFTYRNIADHLARINQFSDLGAQK
                     LYAQRRKCRLRHLIFVPPGRFIKSYFLRRGFLDGFAGLVIAVLNGYGIFVRYAKLREI
                     WKKGERIEPFPD"
BASE COUNT         1883 a         3273 c         3472 g         1770 t
ORIGIN      
        1 gaagacggca tagccgatcg aggggacgtt ctccgcccgg aacatcaaga tgttcgggtc
       61 gaggatctgg aagggaattt ccgctccggt ccggttgtcg atcagacgga accgaccctc
      121 cagctcggcc aggaggcggg gcagaatgac ccggacgacg tctgtccggc tccaggataa
      181 ggggttgaaa acggccaggg gcaggccgtc gccggtcgta atgccgcggg ccaggacggc
      241 caagctccgg tcccggacgg agccggcgat ttcccgggcc tcataggcat gggacgattt
      301 ttgaatccac tggccgcgga ccgactccgc ctccggctcg ctgatggaat cctgcgagcc
      361 ccacgtgtgc tcgtcaaaca gcatggcctg ggcatagccg tcccggatct cggctttcgg
      421 gaacggcgcc ccgctctcaa ccagggaggc ggccgcggcc agcttttcag ccgtcgttaa
      481 atcgtcgtgg gccaagcggt tgatcccggt ttcataagcc gtcgagccgg cgccgtccgt
      541 ccagtaatcg ggccaggctt ttttatgggc ggggagggtc gcgccgtatc gtttctccag
      601 aacccggaaa aattcgctca tcgtcgccag gcggagccgg ggataggccc acttcgcgtt
      661 ccattcggcc acccggtcgg acaactcccg tttcggcgga ctgttgtcca ccggccggcc
      721 gctgatcgga aaagcgatca ggtcataggg atagtccccc cgggcttgga ggccggacag
      781 gtattcagcg aggcgcggct cgctttcggc gtttccttga tgaagacgga gctccaggtt
      841 ggaaaacagg taatgctcgc cgttccagac gaggactttc gccccgtcgg gggattgcca
      901 gtagaaggga ttcggcttgc gaagcggggc tcgggcccgc gtctcgttga tgccgacggc
      961 gaaataatgg attcccgctt ggctgaggat ttggggggaa gcccagctga acccggtgac
     1021 gtcgttgttc atcgccgccc caagggggaa ggccagggtc cggctgaaat ccttggccgg
     1081 caggacggcc cggacgagct cctcgtggcc gaacagatcc gaaagctgga cataccagcc
     1141 cgaaagctcc actcggccgg aacgcagcag gccggccagc gcctggacct tctccgccgg
     1201 ccggctcttg atgtaatttt ccaaggccca ggcgatctcg atgttccagc ggaatttcgc
     1261 ctcgtcggga taggcctcgg tcgcccggca gaactcgatg acccggtcca agtattcggc
     1321 ttggttcttg gccgcccggg acggaaaatc ggtatagccg aggtccgtgt gggaatgggg
     1381 ccagaggtaa accgtccatt tccgctgggg cgcgagaaca aggctccggg aggccgtgct
     1441 gtttcccacc cgcacagaaa accgcgccct gaccggccgg gcgtgatcgg ggagatgaac
     1501 gacgacggag tgcctgcccg gcccgatctt cggaagggct tggcggatct cggcggcgaa
     1561 tcccctgatc cgcagctctc cgggcgcctc cggtccggca tactcgagaa ccgcctcgca
     1621 cttctgaaga agcgcgcccg cttcctcgac gaagaagacc gtcggccgga catccagctt
     1681 gagccggccc gcggccggcg gcggaccggc gaaggatcca acggccgccg ataaaaaaga
     1741 agccagcacg caggtccata acggacgttt aaaaaatcgc atcggggccg cctccttgtc
     1801 cggtcaagcc ggcttcattg tacacgaacc gccgcgaaaa cggacatcat ttcccggcgg
     1861 gccggtttgg gttagaatgg acgggaggca tgtcatgagc gttatttttc ggagggcggc
     1921 ggcggccggt ctcggcctgg ccgtcctggc cgccggcctt ctcccggccg gggcatcgat
     1981 gccgtccttg tcctggacga tccaaacgag cggaacgtcc gaaagcctgc gcggcctgtc
     2041 cgccgtggac gagcggaccg cgtgggtttc cggatcaaag ggaacggttc tccggaccgt
     2101 cgacggaggg gagacctgga cggccgtccc ggtcccgggc gcggaagagg ctgatttccg
     2161 cgacatcgag gccttcggtc ccgaagaagc cgtcgtcatg gccgccgggc gcccggcccg
     2221 cctcttccgc acggccgacg gcggccggac atggacggaa acgtatttcg acgactcccc
     2281 cgggatcttc ctcaacgcct tggccttctt cgacgggatg aacggcttgg cgctcggaga
     2341 tccgatggac ggccggttct ttcttctccg gacttcggac ggaggaagga cgtggcgttc
     2401 cctcccgctg gaatcccgcc cggccgccct cgagggcgag gcggcgttcg ccgcctccgg
     2461 aacggccctt cacgtccggg ggaacgcggc ttggttctgc acgggcgggg cggcctcgcg
     2521 gatatggctc tcctccgacg ggggcgtccg atgggaagcc gtttccgctc ccctcggcgg
     2581 cggaaccccg tcgtcgggag gattctccgt ttattttctc aacgaaaagg ccgggatcgc
     2641 cgtgggcggg gattaccgga acgagccggc cgacgcggac aacgccgccg tcaccttcga
     2701 cgggggaaaa acatgggcgc cggtttccgg ccggcggccg ggcggattcc gggaagccgt
     2761 cgcggccgtc ccggaagacc catcgtctcc ggccgtgacc gtcggcccgt ccggttcgga
     2821 cgtatcgttc gatggaggac aaacctggat cgcgatcggc ggtcccgaag gatttcatac
     2881 cctcggtttc gccggcaacg ggctggcggc ctgggccgcc ggccggaacg gcctgatcgc
     2941 ccggctcgcc tttcaataag cggcctcatc ggctttccaa gagagccaag ccgatccgcc
     3001 ctcagaagaa tcccgatcag cacgggcggc ggatcctgag agcgaccgcc ggatcttcgc
     3061 cgtggcggac gcttgcaggc ggagggccga gcctcccgcc ccgcccctca tccgaggcgg
     3121 cgcgtcccat cgtccttgaa aacgagggcc ggctcactcc cgatggaaat cgtcgtcatt
     3181 ccctctcagg cggacgccga gggggccggc cgagcaggtt tcggatggaa ttttgaagga
     3241 ctaaacgctg atcgctccct cgccgtcgat cgtccgttcg acgtaaaggt agcgcttgat
     3301 tttttgggtc ggcgttttct cgaacggctc ggtttgctcg atgacccggc tcaaccggga
     3361 gaacgaggcc acgccttcgt tgaccttcgc cttgatgtcc tcgagaagac ggcggatgtg
     3421 ctcgcggatc tgggaggcgc tccatttttt ctcggagaaa aacgcgtcca gggcttcgta
     3481 gttcaggtgg atccgggcca ccaatcgccc ttgctcctcg aagaccagag actcctggac
     3541 ggagtcggtc cggttgatga ccgattcgat gacctcgggg tagatgttct ccccgctcgg
     3601 cccgatgatc acgtttttcg accggccctt gatgtacagg aatccgtcgg cgtccaggca
     3661 tcccaggtcg ccggttttca gccagccgtc ggagaaggct tcggcggttt tttcgggatc
     3721 cttgtagtaa cccttcatga tcatcggacc gcggagctgg atttctcctt cgcccgtctt
     3781 gggatcggga tcggcgatcc ggatctccac gccctcgaga ggccggccgg cggaccgggg
     3841 ccgggtttca aacggcttcg tccccgtgac caggggcgcc gtttccgtca atccgtagcc
     3901 gatgcagtag gggaaacccg cttcgatcag gaaatcctcg acgtcggggg ccagggccgc
     3961 cccgccgatg cagaacatat tcaatttccc gccgaacgcc gcccggattt tcgccccggc
     4021 cttgcggatg accatcttcc gcagcgccgg gacggcgcag gccatccgga tcagggcttt
     4081 tccccggaga gcgggaaaga ccttggcccg gaccattttt tcgatgatca ggggaacgga
     4141 caggacgaag gtcggctgga ccgcccggag ggcgggaaga agcaccgcgg ccgtcggcgg
     4201 cttgtggacg taagagaccg aagccccctt catcaagctc gtgatcaatc ccagggtgca
     4261 ttcatagacg tgggccaggg ggaggagcga cagcaagcgg tcctcgcagt tcacatcgac
     4321 gaggcgggtc gtggccagga catcgctgac gaggttcccg tgggtgagca tgacgccctt
     4381 ggaatgcccg gtcgttcccg aagtgtagat gatcgaagcg aggtcctcgg actggacctg
     4441 cgaggggatc cggcccagcc acttcaaggc catcgccttg atcttgcgga gctcgtgact
     4501 gccctcggcg atcagccggc ggagcttgtc cttgctcgta tccggtttga tcacggaaaa
     4561 atcgtcgatc aggatccgcg tcttgagctg gtcgaaggac gcctcgtcga tcttgccgta
     4621 cagcttctcg gagacaaaga cggccttggc ctcggaatgc cggagaacgt gatggatttc
     4681 cgtcggactg aaatccgtca ggatcggcac ggcgacggcg cccatggagg tgacggccag
     4741 gtaggcgacg ccccagtggg gactgttctc gctgaggatg gcgacccggt cgccatggac
     4801 gattccccgg tcggccagga aggccgcgat ctgatcggtt ttatccttca gctgccggta
     4861 gctcaaggcg ggcgaatcgt agaaaccgag ggcgtttctg ttttcataga gctccgcgct
     4921 cttggccaga atggccggaa tcgtcaactc gtttttatgt tccatgtgac tcctcgagtc
     4981 ccggcgcgcg cccggtcggc ggcccggcaa cgcgggcatt gtaacgcaac cccggaccga
     5041 aaacaaaggc acgaatcgcg tcgtccctga tccgatcgat catctccggt caataatcga
     5101 aacggcgggg cgatctcctc ctcccggata ggttctcaag cggtttgacc aggggaatcc
     5161 gcgcggccgg cggatcagaa ggcggccgga cgaccggcct tggcggggac cgggaggtga
     5221 cagggagtgc aatcgatcgg caggtgggaa tggggccggg agcggaaaag atcaaaatcg
     5281 gcctcggaaa aagggacgcc gaggagaatc gtagccagat cctcctccaa ggtattgtcg
     5341 gggacggcca cgatccggcc tttctccttc ccttccacct ggacgatgat cgtcggaacc
     5401 cgttccacgc cggcccggcg cgcctccggc ggttcccgca agtcccggga aacgccgatc
     5461 caaacgacct cgatggccgg attaaaggct tcctcgagcg tcttgagaag ggccgcgatc
     5521 agctcttgac cgttctcgtc ccacgtcccc aggaagactt cgatccgggc gggattggcg
     5581 aagccgcgaa ggccgtcgat cgcccggaaa tggggctcgt actcccgggc ggcccgggcc
     5641 gctccgggga gagcctccag gacgcgttcg gagtccagga ggccggtcag aacgaccgcg
     5701 gccgccggcg cggcggcggg aacggcgatc gactgaatga acagggcgac ggcggcggca
     5761 ccgaaagcgg gccggaggaa agtcggggtt ttcatcgggg gacgtttttt cccggccagt
     5821 ctaagccatc ggccggtcga agtcaaaaca gaggagacta taggccaaga aaggccattc
     5881 cgaggccgca ggcgagaatc gtgaagccgg cgacggcatg gagatagcgt tcaaggaatc
     5941 tcatcgggag caagcggagg ccggcggccg gaagcaggac catcatggcc atggtcgcga
     6001 tggtcgccag gctgaagacg gcggccacga ggacgacgcc gccggtgctc tcccgggcgg
     6061 ccggaaacat cagcaggggg atcaagggct cgcaggggcc gaagacgaag atcgtgaaaa
     6121 gaatccacgg cgtcacgttc accggcttcg gttcggcgtg gacatgtccc gcccggacgc
     6181 cgtccgttcc ttcggcggag gaatgatcgc cgtggacggt gccgtcggtg tggacgtgaa
     6241 gatgatgatg gccccgattc ctcaagcccc gggccaaccc ccagaggaaa tagcccagtc
     6301 cgaagaggat gaacagcccg gcggccacgc tgccgcggac gccctcgaaa atctccagct
     6361 tggccacgcc cagcccgaac aggatgccga cggcgccgat caggatggag ctgccgacgt
     6421 ggcccaagcc gcagagggcc gtaacggcca gagtcttcgc catggaccat ttccgggcct
     6481 tggccaggac gataaacgga aggtaatgat cggggccggc cgcggtgtga agaacgccga
     6541 gagtggcggc cgtggagacg agggcggtca atccgggcgt catcgaggtt ttccgtggca
     6601 cgaatcatac catctgtgcc acaaaagatc aagggcgcgc ggattccgga ccgagagagg
     6661 atcagcccgg ccggaattcc tcccggcgga cggtcgcccg ggcgatccgg gcggggacgg
     6721 ggttgacgcc gatccccggc ccctcgggca cgcggatcgt ccccggcccg ctcaactcga
     6781 tgaacggatc gatgatgtct tcccgataat aacgccggct ggccgagaga tcatggggca
     6841 gggagaaatt cgggagggag gcgaggtgaa ggttgtgggc ccgcccgatt cccgattcga
     6901 gcatcccgcc gcaccacacc gggacgccgg ccgaccggca atggtcgtgg atcgccctgg
     6961 cctcgacgac gccgccgacc cggccgacct tgatattgat gatgcggcag gcgcccatct
     7021 cgagagccgc ccgggcggtc gccagcgaga ggatgctttc atccaggcag agcggcgtgc
     7081 tcatcttctt ttggagcttg gcatgctccc agaggtcgta cggggcgtac ggctgctcga
     7141 caagcaacag ccggaattcg tccaaggcgg cgagggcgga cgcgtccccg ggcccgtagg
     7201 cgccgttggc atcgacttgg agaaggatgc cgggatggcg ttcccggacc gcccggcagg
     7261 cggccgcgtc ccagcccggc ttgatcttga tcttgatccg gcgatatccc tggccgagat
     7321 aggagccgac ccggtcgacc agctccggca gggaatcctc gatgccgacg ctgacgcccg
     7381 cctcgatttc cgtccgcgtt cccccgtaaa gcttcgccag cggcactccg gccgctttcg
     7441 cctgaagatc ccagaaggcc agctccaacc cggccttggc catccggttg ccccggtaga
     7501 tcgaggccgc ccgttggaat tctcccgggc cggagatatt tttcttgaaa acgagcggaa
     7561 cgagaaagtc tttcaggacg tgccaggccg tctccgtcgt ctcgccgctg taaagggggc
     7621 tttcctcggc cgtgcactcg ccccagccgg ccagcccctc ggcctcgacc ttgatcagga
     7681 tgaacgtccg gtcataagac cggccgaagc tggtctcgaa aaaatggaca tagggcaggc
     7741 gaagcaggaa aagctcgaga cgctcgattt tcatgagggc cgtccaaaga agggcgctcg
     7801 ttccgttccg gcggtcagcc ggaggacgta ataggagcgc tcgccggaga tgaaatcatc
     7861 cagacggtat cctcgggcaa agtaagcctc gaacgccttc cgggcggctg tctgccaagc
     7921 ggcgaccagg ccttcgccct ggggcaaatc cgcgattctt cccgggattt cgaccaggat
     7981 ccgcggcact tgaagccgga ggttcggccg gcccgggacg atcgccggct cgaggccgcc
     8041 ctcctccctc tcgagcgctt tcgggatccg ccgcggatcg accggggccg gcggcccttg
     8101 cctccgggcc ctcacccggg ccgacttgat ccgccattcc acgaggaacc ggtcgcttgg
     8161 aacgccctcc tcgacgccga gcgtcggggt ttccccgtag aaattccgga gatacgtccg
     8221 cccgacgact cccaggacat ggatgttgag attggcgttt cgggcttgca gcggatcaaa
     8281 ggtccaggtg acgagctcaa gcctccgttt cagggcttcg tcgcgctgag cccacttcaa
     8341 ggccttgcca aggcccaagc cctggacctc cgggagcacg gccagatggt gggaatgatg
     8401 acccctcgtc cggccgaaat cggccgggaa cgaatagaca tagccggaaa gccgcccgtc
     8461 gacgaacgcc cccagaagga tcgccccggt atggaaggcg atttggaaga aatgatcggg
     8521 gatcaggtcc aggtccggat ggtcccagac ccgccgctgg ataaaaacgc aatcccggaa
     8581 ttcctccagc gacgtcaggg gccggatgcg gacatcaggc ccggagattg attttttggg
     8641 aggcggcgat tttttcatag agatccacga tgcgcgagac catggcttcg gccgagaatt
     8701 tccgccggac ggactcaaag ccttgagccc cgagccgccc ggccagatcc cggtcgttgg
     8761 cgagtctgag gatcgtccgg gccagcgttt cggggtcgcg cggggggatc aggattcccg
     8821 tctccccgtc ctcgacgacc tcggggatgc cgccgacgcg cgtggccacc accggaagcc
     8881 gggcggccat cgcgtccatg atcgaagatc ccaatccctc ggcgtcggac gtgaggacga
     8941 acgcgtccag cgaggccagg aggcgcggga cgtcatcgcg gaagccgagg aaaaagacga
     9001 ggtctccgac gcccaacccg cgggcttgtt cggccagccg gagctcgaga gccccgctgc
     9061 cgatgatgat gatcttgatt ttggggagga acgttttcag gagcttggcc gcctcgatca
     9121 aggtcccatg gcccttgccg tccacgagct gggcgacgat cccgaccagg aagctgtccg
     9181 ggggaaagcc gaactcgcgc ctaaggtaat ccctgtccaa gctcccggcg aacggctcga
     9241 ggtcgatgcc ggaggggatg acttcgatcc ggccggccgg cacgccgccc tccaggagga
     9301 cgtcccggac acgctgggaa atggcgatga ccgcgtcagc ctcggcgtat ttgttccggg
     9361 agaagcgatt cgccttgagg ggatattcga cccgccgggt gacgatccgg acggggacgc
     9421 gcgccaggcg ggcggcccgg ccccccactc cggcggcgtg gccgtcgtgg aaatgggcca
     9481 ggaggcagga ctcgcggcgc atcgcccggc cgattttcca tccggcctgg atgtgggcgt
     9541 cgttcttgat ctcgacgggg aggacccgga gaccggcctc agccgccttt ttctcaagcg
     9601 ggctttcggg ctgggcgacg aggaggaccg ggtatccgcg gccggcaagc tcccgggtca
     9661 gtaaaaacgc ttggcgctgg cctccgcgcc aatcccgccc cgcgtcaatc tggaaaaggc
     9721 tcaatgcgct cccctttttt ccagatttcc cggagcttgg cgtaccggac aaaaatcccg
     9781 tatccgttga gcacggcgat gacgagcccg gcgaagccgt ccaggaaacc ccgccggaga
     9841 aaataggact tgatgaaccg ccccgggggg acgaagatca gatggcggag acggcatttc
     9901 ctcctttggg cgtaaagctt ctgggcgccc aggtcggaga actgattgat ccgggccaga
     9961 tgatcggcga tgttgcggta cgtgaagtga agaaggtcgc cgtgaagctt gcggatctcc
    10021 ccctcgacga ccaggtcctc gtggacatac gccccttccc agcgggcccg gtccttccgg
    10081 aacagccgga ctttccggtc gggataccag ccggaatgct tgatccagcg gccgaggtaa
    10141 tacacccgcc ggggcaccga gaatcccgag caatcggggt cgcccggacc gagggctttg
    10201 atctcctcgc gcaattccgg cgacagccgc tcgtcggcgt cgagggagag gatccaagga
    10261 tgggaggcga gtccgtcggc gtagtttttc tggtcggagt aattcgtcca cttccgctgg
    10321 aagatcctcg tcgtgaaccg ccgggcgatt ttgacggtca aatccgtcga gtcgctgtcg
    10381 acgacgacga tctcggag
//
LOCUS       DAOOXH010000089        10273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_7031_length_10273_cov_7.604228, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000089 DAOOXH010000000
VERSION     DAOOXH010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 10273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10273
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7031_length_10273_cov_7.604228"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1898
                     /locus_tag="PKV64_09405"
     CDS             <1..1898
                     /locus_tag="PKV64_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPN17329.1"
                     /translation="TNIPPHNLGEICNGIVHLLDHPDASLEDIMKLVPGPDFPTGGYV
                     FNQQGIRDAYASGRGTIQVRAKTTIERGDKGERERIVITEIPYAVNKARLIESIADLV
                     NAKKIEGIADLRDESDREGIRIVVEVKRGELPEVVLNNLYKHTSLQTSFGIIMLAIVD
                     KQPKILGLIDMMKYFIGHRQDIVRRRTRFDLKKAEHRAHILEGLTIALDHLDAIIKLI
                     RSAPTVDAARAGLMEKFKLSQIQAQAILEIQLQRLTHLEREKIVQEYTEVKALIVKLK
                     KILSSDKLVTEIIAGEMKEIKETYADARRTELRDEVVTDIRAEDLIKEEDVVVMTTQS
                     GYIKRTSLSSYRFQMRGGKGRKGISMKAEDVVDDVFVCSTHSYLLVFTNIGRMYWLKA
                     LEIPDVGVAGRGKAIANLLEFQSNERATSVVSIKDFSEDHYVVMLATDGQIKKTRLSE
                     FKNIRRGGIIAMNLRPKKELMWTRLTDGKRDLVLATRLGKAIRFKEKDIRPMGRTAAG
                     VRAMTLAKKDEIIGFVAVGDEDKYIFTASEKGYGKRTEAASYRIQHRGGKGLINLKIT
                     PKIGKAIAVLGVAEDDLLVVTVSGKVIRIKTEQIRSTGRATQGVRLINLEENDAVCAV
                     AKARETD"
     gene            2054..3679
                     /locus_tag="PKV64_09410"
     CDS             2054..3679
                     /locus_tag="PKV64_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPN17330.1"
                     /translation="MAPTGRKGFSFLATLPSGSKTPFRAVSGVVLVGRRLPPGPDHWS
                     LEDEMVSARHARLTLDDGGVWIEDLQSANGTWVDGKRIQTRTLLDPASIVRLGRTTLR
                     LAAVPSAAPAPPPFPPKGKTPLPFQPPAEALAGDESVPNLVLGGATAEAVRERLTAVF
                     DLTAALSGIDSVETLASHLLEHLHRAFASFGPNTHSGLLVGPELILKAYRPETDPPTC
                     SLTLGRHVFSEKKACLWRLGAAGEVDPSMSLMASGTTSALYAPLAWAGEVYGVVYFDL
                     TSGTKTFGRDDLRLVQLMAAQTAMFIKNLQLGQVLQREAAIKTRLLAQFPPSIAERLA
                     RLPDHTAVPSERLDRVTVLISDVRGFTKLAAAMEPEAVVRMLNDMFHELTPIILKHNG
                     TVDKYIGDAILAVFGCPEPDEQQAERAVQAALEMQEAVRRLETKRWKGRAPFRIGVAV
                     HTGPAIHGFIGAPERMEYTVVGNTINLTSRYCDAAAPGTILVSPEIYQRLHYCLDVEH
                     PPLEVETKHEGPIKAYVVRGWKGGRPAGKEGAP"
     gene            3676..5562
                     /locus_tag="PKV64_09415"
     CDS             3676..5562
                     /locus_tag="PKV64_09415"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPN17331.1"
                     /translation="MTASDTKKKTKGTGAGRTPGPASKTEAVRICPACRAENAASSRF
                     CGSCATPLQDTGAPLEAAPDGPRTVRASTVELARGNRLAGRYEIIEELGRGGMGKVFK
                     AYDHKISEVIALKLIRPEISAQEKAIERFKNELKFTRRITHRRICRMYDLGEEDFLYY
                     ITMEYVAGEDLKRFVRRAGPLNAGKAVMIAGQICDGLAEAHRIGAIHRDLKPQNIMID
                     ADGNAKIMDFGIARFTEMERVTGSGVMIGTPEYMSPEQAELGEVDARSDIYSLGVVLF
                     EMVTGRVPFEGATPLSLAMKHKMEVPRNVREWNAQVPAGLAAVIARCLEKDPAKRYPS
                     AEELKAALEDAGRDIPTAEWPAPAGAAAAKTPAAPGARKIPVPLIAAGVVIAAAAVYF
                     IFSKPGAPPVPASETAADTGSSAALPGDPFPSVVMKEAATETRTTESRPAPPETVKSP
                     QIPKPVSSESRSAAPPAKPPAVSPLAPEEQKAVDMALARMTGARSLAVKQGHGSGSAF
                     FEAAEMLREKGASAAGAGKGTDARCSFTISEKMFRLCVTEKRDPARWRELAKLVDRLR
                     ENALEAWKNVPADPLFQEARSRSTRGDAARAKDDLAGAVKEYALAAFDYEKIRWTVEA
                     ALKK"
     gene            5668..7458
                     /locus_tag="PKV64_09420"
     CDS             5668..7458
                     /locus_tag="PKV64_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPN17332.1"
                     /translation="MPKTLPEIFLNTVRSYVKPDLMKVKRNDVYSDISTSEFESAVRN
                     LALGLKDLGHRPGAKLVILSENRPEWTITDLANLCLGGVTVPIYTTLTGDQVKYIIED
                     SDAEIVVVSNFYQYQKIAALRRDLPRVKHVVIFQPQAPAPDVLPLAAVQARGAALEAA
                     RPGLFEETARAVQPDDEASIIYTSGTTGVPKGVILTHANFVSNVLAVSGIIEFSAADT
                     ALSFLPLSHVLERMVSFTYLYKGCSIAYAENVETVAHNLLEIRPQVMVSVPRVFEKIY
                     SRVMDQVLSSPGLKRKIFFWGIGAGKAAGEKKLAGKTAGPLLGLKLALARKLVFSKIL
                     DKTGGRVRFFVSGGAPLSKDIAEFFYAIGLTILEGYGLTETSPVLAVNTFDALRFGTV
                     GRVLPGVEIRIAPDGEISAKGPNIMKGYYKKPDETRDVFEDGWLKTGDIGFLDADGFL
                     TITDRKKDLIVTAGGKNIAPQPIENILKTNPYITNAVVIGDRRRYVTALIVPNFEKIE
                     EYAKFFKISYRDRADLIRNEQIVNFIRSEIERATPNLASYERIKKIALLDREFDIAQG
                     EITPTLKVKRNIIESRYQEMIEDLYKDDAY"
     gene            complement(7505..8251)
                     /locus_tag="PKV64_09425"
     CDS             complement(7505..8251)
                     /locus_tag="PKV64_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPN17333.1"
                     /translation="MDISFDKLIKLQKIDSEIISVNAHLDSIPDQIEAIDEDIKATAV
                     LVSGARDKLAANQKKRRELEGEVKGIREQTAKFKRQLNDVKTNKEYSALLKEIEETDQ
                     KIARIEEEILNEMIAADDIEKEIKAALKKQGEEEGKLKIERDQALAEQKEIEKKKDRL
                     EKDRSALLPAIPAEQLSIYQRIAKKTAGVALSPVTDDFCSICQLRIRPQILDDLWAMN
                     EIITCEACGRILYHRKPADPPDSDPEKETG"
     gene            complement(8270..8341)
                     /locus_tag="PKV64_09430"
     tRNA            complement(8270..8341)
                     /locus_tag="PKV64_09430"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8307..8309),aa:Ile,seq:cat)
     gene            complement(8443..10068)
                     /gene="rpoD"
                     /locus_tag="PKV64_09435"
     CDS             complement(8443..10068)
                     /gene="rpoD"
                     /locus_tag="PKV64_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HPN17334.1"
                     /translation="MAADNKFPKDEISELISKGRRQGFLLYEDIDKTFQDDLDHGANF
                     DDFLSSIDDYGIKILDTKGSELVPRRRKNEVVSTQGLERTTDPVKLYLREMGNISLLT
                     REGEIAIAREIERGEKAIVKALSKTRLVLNEVLALEEKIKIDPMIITEVFDVSEDDVA
                     EGKLEEKKKFILAKIKKIKDLGRKLDKIPNTKRMVFTRSRIIVAMSSQIRDLNMRAIQ
                     QERIIEILRARLRIINDLEERREELEALRNKTRKKKTAQDLEPQIKEIAKNLRAAQKD
                     IGLDAPHLRRIIRAITTGKKISDQAKKELVAANLRLVVSIAKKYSNRGLQFLDLIQEG
                     NMGLMKAVDKFEYRRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLIRI
                     SRQLVQEIGREPTSEEIARKMDMPVGKVRKIIKIAQEPISLETPIGEEEDSHLGDFIE
                     DKVMPSPPDAVIHINLREQIEEALKSLTEREAKVLKMRFGLGDGNEHTLEEVGQQFKV
                     TRERIRQIEAKALRKLKHPSRSKKLRSFTNGFG"
BASE COUNT         1859 a         3294 c         3208 g         1912 t
ORIGIN      
        1 ccaccaacat cccgccccat aacctgggcg agatctgcaa cggcatcgtc cacctcctgg
       61 atcatcccga cgcctcgctc gaggacatca tgaagctcgt ccccgggccg gatttcccca
      121 ccggcgggta cgtcttcaac cagcagggca tccgggacgc ctacgcctcc gggcggggca
      181 ccatccaagt ccgggccaag acgacgatcg agcggggcga caagggcgaa cgggagcgga
      241 tcgtcatcac cgagatcccc tacgcggtca acaaagcccg gctcatcgag agcatcgccg
      301 accttgtcaa cgcgaagaaa atcgagggca tcgccgacct ccgggacgaa tccgaccggg
      361 aaggcatccg gatcgtcgtc gaggtcaagc ggggcgagct tcccgaggtc gtcctgaaca
      421 acctctacaa gcacacgtcg ctccagacgt cgttcgggat catcatgctg gccatcgtcg
      481 acaagcagcc gaagatcctc ggcctgatcg acatgatgaa gtatttcatc ggccaccggc
      541 aggatatcgt ccggcggcgg acccggttcg acctgaagaa ggccgagcac cgggctcaca
      601 tcctggaagg cctgacgatc gccctcgatc atctcgacgc gatcatcaag ctcatccggt
      661 cggccccgac cgtggacgcg gcccgggccg ggctgatgga aaagttcaag ctgtcccaga
      721 tccaggccca ggccattctc gagatccagc tccagcgctt gacccacctc gagcgggaga
      781 agatcgtcca ggaatacacc gaggtcaagg ccctgatcgt caagctcaag aaaatcctct
      841 cctcggacaa gctcgtgacc gagatcatcg ccggggagat gaaggagatc aaggagacct
      901 acgccgacgc ccgccggacc gagctgcggg acgaggttgt gaccgatatc cgggccgagg
      961 acctgatcaa ggaggaggat gtcgtcgtca tgacgaccca gtcgggctac atcaagcgga
     1021 cctccctctc ctcctaccgg ttccagatgc gcggggggaa ggggcgcaag ggaatcagca
     1081 tgaaggccga ggacgtcgtc gatgacgttt tcgtctgctc gacccactcc tacctcctgg
     1141 tcttcacgaa catcggccgg atgtactggc tcaaggccct cgaaatcccc gatgtcggcg
     1201 tggccgggcg cggcaaagcc atcgccaacc tcctcgagtt ccagtccaac gagcgggcga
     1261 cctcggtcgt gtcgatcaag gatttttccg aggaccacta cgtggtcatg ctggccaccg
     1321 acggccagat caagaagacc cggctgagcg agttcaagaa catccgccgc ggcgggatca
     1381 tcgccatgaa tctccggccg aagaaggagc tcatgtggac ccggctgacg gacggcaagc
     1441 gggacctcgt gctcgcgacc cggctgggca aggccatccg gttcaaggag aaggacatcc
     1501 ggccgatggg ccggaccgcc gccggcgtcc gggcgatgac cctggccaag aaggacgaga
     1561 tcatcggctt cgtggccgtc ggcgacgagg acaagtacat tttcacggcc agcgagaaag
     1621 gctacggcaa gagaacggag gccgcctcct accgcatcca gcaccgcggc ggcaaggggc
     1681 tcatcaacct caagatcacg cccaagatcg gcaaggccat cgccgtcctg ggcgtcgcgg
     1741 aagacgacct cctggtcgtc accgtctcgg gcaaggtcat ccggatcaaa accgagcaga
     1801 tccgctcgac cggccgggcc acccagggcg tccgcctgat caatcttgag gaaaacgacg
     1861 ccgtctgcgc cgtggccaag gcccgcgaaa cagattgaat cctccggcgg cggggggatt
     1921 tcccggcgcc gagacgtccc tcccccgcgg accggatttt gactttttcc tccattcgga
     1981 gttataaaaa ccgcatcatc ggagacgctc ctccgggcgg aagatcgtcc ccggatcaga
     2041 ggaaggagaa cgcttggcgc cgaccgggcg gaaaggattc tcgtttctgg cgaccctgcc
     2101 ctccgggtcg aaaacaccgt tccgcgccgt ttcgggcgtt gtcctcgtcg gccgccgcct
     2161 tccgccgggg ccggatcact ggagcctgga ggacgagatg gtctccgccc ggcacgcccg
     2221 tctcaccctg gatgacggcg gcgtctggat cgaggatctt caaagcgcca acggaacctg
     2281 ggtcgacggg aagcggatcc agacccggac gcttcttgat cccgcctcga tcgtccggct
     2341 cggccggacc accctgcgcc tggcggccgt cccttcggcc gccccggctc ccccgccgtt
     2401 tccgccgaaa gggaaaacac ccctgccttt ccagccgccg gccgaggccc tcgccgggga
     2461 tgaatccgtc cccaacctcg tcctgggggg agcgacggcc gaggccgtcc gagaacggct
     2521 gacggccgtt tttgatctca cggccgccct aagcgggatc gactccgtcg aaaccctggc
     2581 ctcccacctc ctggagcatc ttcaccgggc tttcgcctcg ttcggcccga acactcacag
     2641 cgggctcctg gtgggaccgg agctgatcct caaggcctac cgccccgaaa ccgatcctcc
     2701 gacctgcagc ctgaccctgg gccgccacgt cttttccgag aagaaggcct gcctttggcg
     2761 cctgggggcg gccggagagg tcgatccgtc gatgagcctg atggcctccg ggacgacctc
     2821 ggccctctat gcccccttgg cctgggcggg agaagtctac ggtgtcgttt atttcgattt
     2881 gacgtccggg acgaaaacct tcggccggga cgacctgcgg cttgtccagc tgatggccgc
     2941 ccagacggcc atgttcatca agaatctcca gctcggccag gtcctccagc gcgaggcggc
     3001 gatcaagacc cgcctcctgg cccagttccc gccctccatc gccgagcgcc tggcccggct
     3061 tccggatcac accgccgtcc cctccgagcg cctcgaccgg gtgaccgtcc tcatctcgga
     3121 cgtgcgcgga ttcaccaagc tggccgcggc catggagccg gaagccgtcg tccggatgct
     3181 caacgacatg ttccacgaat tgaccccgat catcctcaag cacaacggga cggtggacaa
     3241 gtacatcggg gatgcgatcc tggcggtttt cggatgcccc gagcccgacg aacagcaagc
     3301 cgagcgggcc gtccaggccg ccctggaaat gcaggaggcc gtccggaggc tggaaaccaa
     3361 gcgctggaag ggccgggcgc cgtttcgcat cggcgtcgcc gtccacaccg ggccggccat
     3421 ccacggcttc atcggcgcgc ccgagcggat ggaatacacg gtcgtcggca acacgatcaa
     3481 ccttacctcg cggtattgcg acgcggccgc tccggggacg attctcgtca gccccgagat
     3541 ctaccagcgg ctgcattact gcctcgacgt cgagcatccg cccctcgagg tcgagaccaa
     3601 gcatgaaggt ccgatcaagg cgtatgtcgt ccgcggctgg aaggggggcc ggccggccgg
     3661 gaaggaaggt gccccatgac cgcttccgac acgaaaaaaa agacgaaagg aaccggggcg
     3721 ggccgaaccc ccggcccggc ctcgaagacg gaagccgtcc ggatctgccc ggcttgccgc
     3781 gcggaaaacg cggcttccag ccggttctgc ggcagctgcg cgacccccct ccaggacacc
     3841 ggagctcccc tcgaggccgc ccccgatgga ccgcggacgg tccgggcctc gaccgtggag
     3901 ctggcccggg gaaaccggct ggccggccgc tacgagatca tcgaggagct cggccgcgga
     3961 gggatgggga aggtgttcaa ggcttatgat cacaagatca gcgaggtgat cgccctcaag
     4021 ctcatccggc cggagatcag cgcccaggag aaggccatcg aacggttcaa gaacgagttg
     4081 aaattcaccc ggcggatcac ccaccgccgc atctgccgga tgtacgacct gggagaggag
     4141 gacttccttt actacatcac gatggagtat gtcgccggcg aggacctgaa gcggtttgtc
     4201 aggcgggccg gcccgctcaa cgccgggaag gcggtgatga tcgccggcca gatctgcgac
     4261 ggactggccg aagcccatcg gatcggagcg atccatcgcg atctcaagcc ccagaacatc
     4321 atgatcgacg ccgacggcaa cgccaagatc atggacttcg gcatcgcccg gttcaccgag
     4381 atggagcggg tgaccgggtc cggggtgatg atcgggacgc ccgaatacat gtctcccgag
     4441 caggccgagc tgggcgaggt cgacgcccgc tcggacatct attcgctggg cgtcgtcctc
     4501 ttcgaaatgg tgaccggaag agtgccgttt gaaggagcca cgcccctcag cctggcgatg
     4561 aagcacaaga tggaggttcc ccggaacgtc cgggaatgga acgcccaggt tccggccggg
     4621 ctggccgcgg tcatcgcccg gtgtctggaa aaggacccgg ccaagcggta tccgtcggcc
     4681 gaagagctga aagcggcctt ggaggacgcc ggccgggaca tcccgacggc cgaatggccc
     4741 gccccggcgg gcgcggcggc ggcgaagacg ccggccgcgc ccggagcaag gaaaattccg
     4801 gttccgctga tcgcggccgg ggtggtcatc gcggccgcgg ccgtctattt cattttctcg
     4861 aagcccggcg ctccgcctgt tccggcatcg gagacggcgg cggataccgg atcttcggcg
     4921 gctctcccgg gcgacccgtt tccctcggtc gtcatgaagg aggcggcgac ggaaacccgg
     4981 acgacggagt ctcggcccgc cccgcccgaa acggtcaaga gccctcagat tccgaagccg
     5041 gtttcctccg aatccagaag cgccgcgccg ccggcgaaac cgccggccgt gtcgcccctg
     5101 gcgcctgagg aacagaaagc ggtcgacatg gccctggccc ggatgaccgg ggcccggagt
     5161 ctcgccgtca aacagggcca cggatcggga tccgcgtttt tcgaggccgc tgaaatgctt
     5221 cgagaaaaag gcgcgtcggc cgcgggcgcc gggaagggga ccgacgcccg atgttctttc
     5281 acgatctcgg aaaagatgtt ccgcttatgc gtgaccgaaa aacgcgatcc ggcccgctgg
     5341 cgcgagctgg cgaagctcgt cgaccggctg agggaaaacg ccttggaggc ctggaaaaac
     5401 gtcccggcgg atccgctctt ccaggaagcc cggtcccggt cgacccgcgg cgatgccgcc
     5461 cgggcgaagg atgatctcgc cggcgcggtc aaggaatacg ccttggccgc tttcgattat
     5521 gagaagatcc ggtggacggt ggaagcggcc ctgaaaaaat agagacgcgc cgcggcgccg
     5581 gacggtcctt tcgtaatttt gggttgaccc tgcctgtctt tttccataga atgggcgcat
     5641 tcgttcgcag aaatcgtgga ggacaagatg ccgaagaccc tgcccgagat ttttttgaat
     5701 acggtccgga gctacgtcaa accggacctg atgaaggtga agcgcaacga cgtctacagc
     5761 gacatctcaa cgtccgaatt cgagtccgcc gtccggaacc tggccctcgg cttgaaggat
     5821 ctggggcacc gtccgggagc caagcttgtc atcctgtccg agaaccgtcc ggaatggacg
     5881 atcaccgatc tcgccaacct ctgcctcggc ggagtcaccg tgccgatcta cacgacgctc
     5941 acgggcgacc aagtcaagta catcatcgag gacagcgacg ccgagatcgt ggtcgtctcc
     6001 aacttctacc agtaccagaa aatcgcggcc ctccggcggg accttccgcg ggtgaagcat
     6061 gtcgtgatct tccagccgca ggccccggct cccgacgtgc ttcccctggc cgccgttcag
     6121 gcccgcggcg cggccctgga ggccgctcgt cccggcttgt tcgaggaaac cgcccgggcc
     6181 gttcagccgg acgacgaggc ctcgattatt tacaccagcg gaacgaccgg cgtccccaag
     6241 ggcgtgatcc tcacccacgc caatttcgtc agcaacgtcc tggcggtctc ggggatcatc
     6301 gagttttccg ccgccgacac ggccctgtcc tttcttcccc tgtcccacgt tctcgagcgg
     6361 atggtctcgt tcacttatct ttataagggg tgctcgatcg cttacgcgga gaacgtcgag
     6421 acggtcgccc acaacctgct cgagatccgg ccgcaggtca tggtcagcgt cccccgggtt
     6481 ttcgaaaaga tctattcgcg ggtcatggac caagttctct caagcccggg cttgaagcgg
     6541 aagatcttct tctggggcat cggcgcgggc aaggccgccg gggagaaaaa gctggccggc
     6601 aaaaccgccg ggccgctgtt agggctcaag ctcgccctgg cccgcaagct cgtcttttcg
     6661 aaaatcctgg ataagacggg cggccgggtc cggttcttcg tctccggagg cgcgccgctg
     6721 tccaaggaca tcgccgagtt cttctacgcc atcggcctga cgatcttgga gggatacggc
     6781 ctgacggaga catccccggt cctggccgtg aataccttcg acgccctgag gttcgggacc
     6841 gtggggcgcg ttctgccggg cgtcgagatc cggatcgccc ccgacgggga gatctcggcc
     6901 aaggggccca acatcatgaa gggctattac aagaagcccg atgaaacgcg ggacgtgttc
     6961 gaggacggct ggctcaagac gggcgacatc gggttcctcg acgccgacgg attcctgacg
     7021 atcaccgacc ggaaaaagga cctgatcgtc acggccggcg gcaagaacat cgccccccaa
     7081 cccatcgaga acatcctgaa gacgaatccc tacatcacga acgcggtcgt catcggcgac
     7141 cgccgccgct acgtcaccgc cttgatcgtt cccaattttg agaagatcga ggagtacgcg
     7201 aaattcttca agatctccta ccgggaccgg gcggacctga tccgcaacga gcaaatcgtc
     7261 aacttcatcc ggtcggagat cgaacgggcc acgccgaacc tggcctccta tgagcggatc
     7321 aagaaaatcg ccctcctgga ccgcgaattc gacatcgccc agggcgagat cacgccgacc
     7381 ctcaaggtca agcggaacat catcgagagc cgctaccagg aaatgatcga ggatttgtac
     7441 aaagacgacg cgtactgagg aacggcggcg gaaaacgatt cgtccggcgg atggagccgg
     7501 ggaatcagcc ggtttctttc tccggatcgg agtcgggcgg atccgccggc tttcggtggt
     7561 aaagaatccg gccgcaggct tcgcaggtga tgatttcgtt catggcccaa agatcgtcga
     7621 ggatctgggg ccggatgcgg agctggcaga tcgagcagaa atcgtcggtg acgggggaga
     7681 gggcgacgcc ggccgtcttc ttggcgatcc gctggtatat cgacagctgt tcggccggaa
     7741 tcgcggggag aagcgcgctc cggtcttttt ccagccggtc tttcttcttc tcgatttcct
     7801 tctgttcggc cagggcctgg tcgcgctcga tcttgagctt gccctcttcc tccccctgct
     7861 ttttcagagc cgccttgatc tccttttcga tgtcgtcggc ggcgatcatc tcgttgagaa
     7921 tctcctcctc gatccgggcg atcttctggt ccgtttcctc gatttccttg aggagggcgg
     7981 aatattcctt gttggtcttg acgtcgttca gctggcgctt gaatttggcc gtttgttccc
     8041 gaatgccctt cacctcgcct tcgagctccc ggcgcttctt ctggttggcc gccagcttgt
     8101 cccgggcccc cgagacgaga accgccgtcg ccttgatgtc ctcgtcgatc gcctcgattt
     8161 gatccgggat cgagtcgaga tgggcgttga cgctgatgat ttcggaatcg attttctgaa
     8221 gcttgatgag cttatcaaac gaaatatcca ccgggtcctc gcgatcaaat gggcctacca
     8281 ggattcgaac ctgggacaga ccggttatga gccgggagct cttccgctga gctataggcc
     8341 ctttgggatt ttatatcaaa attgccgatg ggacgcaacg gaattttgcg cccccgggag
     8401 ggggaggccc ggggccgccc cggcggcggg cttcggccgg actcacccga agccgttggt
     8461 gaacgaccgg agttttttcg accggctggg gtgtttaagc ttccggaggg ctttggcttc
     8521 gatctggcgg atgcgctcgc gggtgacctt gaactgctgc ccgacttctt ccagcgtatg
     8581 ttcgttgccg tcgccgaggc cgaaccgcat tttcagaacc ttcgcctccc gttcggtcag
     8641 gcttttcaag gcttcctcga tctgctcccg gaggttgatg tggatgaccg cgtccggggg
     8701 cgagggcatg accttgtcct cgatgaagtc ccccaagtgg ctgtcctctt cctccccgat
     8761 gggcgtttcc agggagatcg gctcctgggc gatcttgatg attttccgga ccttgccgac
     8821 gggcatgtcc atctttctgg cgatttcctc gctggtcggc tcgcggccga tttcctggac
     8881 gagctggcgg ctgatgcgga tgagcttgtt gatcgtctcg atcatgtgga ccgggatgcg
     8941 gatggtccgg gcctggtcgg cgatggcccg ggtgatggcc tgccggatcc accaggtcgc
     9001 gtaggtcgag aacttgtagc cgcgccggta ctcgaacttg tccacggcct tcatcaagcc
     9061 catgttgcct tcctggatga gatccaggaa ctggaggccc cggttgctgt attttttggc
     9121 gatcgagacg accaggcgga ggttggccgc gaccagttct ttcttggcct ggtcgctgat
     9181 ctttttaccg gtcgtgatcg cccggatgat ccggcggaga tggggggcgt ccaggccgat
     9241 gtccttctgg gccgcccgga ggtttttcgc gatctccttg atctgcggtt cgaggtcctg
     9301 ggccgttttc ttcttccggg ttttgttccg gagggcttcg agctcttccc ggcgttcttc
     9361 cagatcgttg atgatccgga gccgggcccg gaggatttcg atgatccgct cctgctggat
     9421 cgcgcgcata ttcagatcgc gaatctgcga gctcatcgcg acgatgatcc gggagcgggt
     9481 gaagaccatc cgcttggtgt tggggatctt gtcgagcttg cggccgagat ccttgatttt
     9541 tttgatcttg gccaggatga acttcttctt ttcctcgagt ttgccctcgg ccacgtcgtc
     9601 ttcgctgacg tcgaagacct cggtgatgat catcggatcg atcttgatct tttcctcgag
     9661 ggccaagacc tcgttgagga cgaggcgggt cttggacagg gctttgacga tggccttttc
     9721 cccgcgctcg atttcccggg cgatggcgat ctcgccctcc cgggtcagga gcgagatgtt
     9781 gcccatctcc cggagataca gcttgaccgg gtcggtcgtc cgctcgagcc cctgggtgga
     9841 gacgacctcg tttttcctcc ggcggggcac cagctcgctg cccttggtat cgaggatctt
     9901 gatcccgtag tcgtcgatgg aagaaaggaa gtcgtcgaaa ttggcgccgt ggtccaagtc
     9961 gtcttggaac gtcttgtcga tgtcctcgta cagcaggaat ccctgccggc gtcccttgga
    10021 aatcagctct gaaatttcgt ctttcgggaa cttgttgtca gcggccacgc gcggctcctt
    10081 ggcgtccgag gtttttcgtc ggccgccgtt ttacgccccc cgccggggga tcgatgggcg
    10141 ttttcatctc aaagccagga cctggcgggt caggtccagc ttccggctca tcaaggactc
    10201 cagcttctcc cgatcgcccg atttttccgc ccgggcgatc tccccttgga tttttcgaag
    10261 agcgttctcc ttg
//
LOCUS       DAOOXH010000090        10262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_7047_length_10262_cov_6.954737, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000090 DAOOXH010000000
VERSION     DAOOXH010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 10262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10262
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7047_length_10262_cov_6.954737"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            354..2795
                     /locus_tag="PKV64_09440"
     CDS             354..2795
                     /locus_tag="PKV64_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HPN17335.1"
                     /translation="MAASREAKNEVSGGVIGGVLGGSAGEKEAGKDSGPPPPVIDLFS
                     IQARVNLNETAFFYPHLLTDAEGTVTLEFKMPEALTEWRFLGFAHTTGLRSGSIEAAA
                     VTQKELMVQPNPPRFLREGDVLEFTVKVTNMTEKEAAGAVELRFFDPRNDAPLDGALA
                     NANPKLSMAIPAKQSRTFSWPITVPDGAETVGFRAVAAAAGFSDGEEGMVPVLSRSLM
                     VRESIPLWISGRGEKRFRFDKLAQSAGSTTLRSLGLTVQMSSNPAWYAVQALPYLMEY
                     PYECSEQVFNRLYANALARKIAGSDPRIRRVFELWKGTPALQSNLEKNQELKSVLLEE
                     SPWVVEAKNESQAKRNIGLLFDENTTAPALKRAVQKLVDMQLPDGSWPWFPGGPGNDY
                     ITLYIVTGFGRLEHLGVPGIAREAVLRAVGRLDSWIREVHERILADKTEKLNNLSPSI
                     ALYLYARSFFLDGCPIPDASRKALDYFLGQGAAFWLQLDSRQSQAHLALGLNRFGDAE
                     TARKITRSLKERSKVDEELGRYWAEGEASWWWRRAPIETQAMMIEAFDEVAGDAAAVE
                     ECRIWLLKQKQTQDWKTTKATADAVYGLILRGADLLASDEIVEVILGGRKVEPEKVEA
                     GTGFYEKRFSAAEIRPEMGEIEVRKTDPGIAWGGVHWQYLEDISKITPHERNPLRLKK
                     SLFKKTFTKKGPEIEPVRGPLAVGDTLVVRLELRTDRDMEYVHLRDHRGSGLEPVNVL
                     STYRFRDGLAYYEATKDAAAHFFIDDLPKGAYVFEYELRVQLRGRYQNGMAHIECMYA
                     PEFNSHSESRVLEVR"
     gene            2895..3230
                     /locus_tag="PKV64_09445"
     CDS             2895..3230
                     /locus_tag="PKV64_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPN17336.1"
                     /translation="MDIVMTTESDVAVFSIIGHLDAVSSPELEGRLGTWFEEPGTRLV
                     LDLNGLEYISSAGLRVILSAAKKMKARNGKLGLSRLQPRVREVFQVSGFSSIIPVFET
                     LPAALDAVR"
     gene            3254..3661
                     /locus_tag="PKV64_09450"
     CDS             3254..3661
                     /locus_tag="PKV64_09450"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN17337.1"
                     /translation="MERLRRPARMEFLHELLEFIDAGAKRLGFDESARGRIRLASEEA
                     LVNVILHAYAGADGEVEAGLSGGEDRSLRIEIRDRGAAFDPLAEAEPDLEAGLADRKI
                     GGLGIFFIREAADRAAYRREGEENILTLYFIRT"
     gene            complement(3648..5102)
                     /locus_tag="PKV64_09455"
     CDS             complement(3648..5102)
                     /locus_tag="PKV64_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HPN17338.1"
                     /translation="MIIVFVLYFALTLCLGLWEARKTKSQSDFVLGGKKLPGWMLALS
                     ERATGESAWLLLGLTGFIYATGLSGIWTGVGCLLGISTAWIVLARKFRAEAEAYKAMT
                     MPEYFAAKFASKANLIRVLSTGIIVFFFVFYVGAQFAGAGKTIQQTFGFSPFWGMIIS
                     AVVILGYATFGGFVSVVAVDTFQSILMLTTLVVTPVILLVKILASPVSPAQAFAAAGP
                     GFGSLTGGLAGFAAGLMIFNNLAWFFGYLGGQPQLNARFMGMKDEKNVRLGRNIAVLW
                     TIIAYSGVVIIGLGAHVLFGPKAVADSEMILPFILNNLFPGWLAALLLAGAVAAMIST
                     AESMLLVAATSISEDVYKGILKKGRARERTLLLISRLSTVAVGVLGLVLALTTKDLIY
                     SIVSYAWAGIGCSFAPAVLLSFYWDRFNAAGVVTALASGLVSTVVWMLAGLEKTVTAR
                     ASTFIIAMTLAVLVTLVSKKKEPETAKASPLTSG"
     gene            complement(5209..5655)
                     /locus_tag="PKV64_09460"
     CDS             complement(5209..5655)
                     /locus_tag="PKV64_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPN17339.1"
                     /translation="MKNVKDILAAKGSKVWSISPHAKVFEALKLMAEKRIGALMVMDD
                     KGRMEGIITERDYARKIILFGKSSEETLVSEIMTPAGKMFHVGPDNTVEECMVLMTAK
                     HVRHLPVCEGEKFTGLVSIGDVVKAIISEKDALIEQLSNYISGKYM"
     gene            5829..6335
                     /locus_tag="PKV64_09465"
     CDS             5829..6335
                     /locus_tag="PKV64_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPN17340.1"
                     /translation="MQHRPHYGNWGRGERIFHKGDFKYLILDLLKEKPRHGYEIIREL
                     ENQFNGFYTPSPGAVYPTLQYLEEMGHVTSQPLDGKKIYTITESGLAFLAEKAKETSE
                     IKAQMKNWWGCYGAEFRDEMRDVMRTLGDLGKTIGQKARQAGVEKMPQVKQALAEAVK
                     EIEKIFKS"
     gene            6513..8576
                     /locus_tag="PKV64_09470"
     CDS             6513..8576
                     /locus_tag="PKV64_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPN17341.1"
                     /translation="MNKRTAAAFLIAAMVFLSAGAAAAEGEADRDGRMQWWREARFGL
                     FIHWGLYAVPAGEWRGKTDYGEWIRDTARIPLQVYDQFVGRFNPREFDAEAWVRAAKD
                     AGMKYIVITSKHHDGFALFDSKQTDFDILATPFGRDVLRELASACRSQGLKLCFYYSI
                     MDWHHPDYLPRRPWEERIRPVPGGDFDRYVAYMKAQLRELLTQYGDIGVLWFDGEWEN
                     TWNIDRGRDLYRYVRSLQPSIIVNNRVGSDRSGMEGFSAARDAPGDFSTPEQQVPGRG
                     LPGVDWETCLTMNDNWGYNRADQNWKSVRTLIRTLVDVVSKGGNLLLNVGPTAEGVFP
                     GPSMERLAAIGRWMRVNGESLYGTTAGPFSDLRWGRSTQKKDGENTLIFLHVFDWPEN
                     GWLNLPPIAEELRTAYVLSAPAKPLPVEFREFGPRIRVPAQAPDADDTVIVLVTGAET
                     KSYAPPRITAAAKSFLDVLEVTITGDPAFTGIRYTLDGSEPTLESPLAAGPVRLTETT
                     VVSARGFIKGVPVTGTAQETFTKTALRPPDDPGGGLKQGLSFAYYEGEWTALPQFAGM
                     NPIRRGEALHIDLSQKRRHENYGFEFKGLIRAPEDGLYAFALESDDGSRLWIGETLVV
                     DNDGVHPSAERGGAIALKAGFHRITIRYFNRTGGEELKVFWKAPSGPKQLIPDRMLFR
                     REARF"
     gene            complement(8614..8817)
                     /locus_tag="PKV64_09475"
     CDS             complement(8614..8817)
                     /locus_tag="PKV64_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009602163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPN17342.1"
                     /translation="MNESRKVLRRSTTNRMIGGVIGGLADYFGLDVSLLRVVYVVGSI
                     LSAAFPGLLVYLIMWVVIPKEER"
     gene            complement(8889..10184)
                     /locus_tag="PKV64_09480"
     CDS             complement(8889..10184)
                     /locus_tag="PKV64_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN17343.1"
                     /translation="MKRFKNLLQKEVKELVTKQLVISLAFTVLLFNAIGQISKKEVQK
                     AVGIQTISVLDQDGSEGSRALLKGLESARFKIVDQTGKGKDEALASAKAGESKLLLVV
                     PAGFGDSLAELKPSEIETYSFMRSFSLIGARGQVVVQSVISALNETLSNDFLAAKLPG
                     ADPVSLKRPIKSRDFVVVRDRTAEGSAGLIAGMLSQQSMLIPIVLMMIVIYSSQMVIS
                     AVAMEKQNKTLETLLTVPIKRTSIITAKMFAAGLVGLISAGVYMFAFKGFIGGMGEEV
                     AAAGARASGTALMQKLGLTFDTTGYLILGLSLFLAILVALAMAMILGVLAEDFRSAQN
                     MIMPLMFMVMIPYFISLFADIDTLSLPVKILVLAIPFSHPFLVSQNIYLGHYGMIAAG
                     LGYMLLVFIVLVIYAARIFSTDKILTMKLRFGKKKAPAA"
BASE COUNT         1966 a         3108 c         3352 g         1836 t
ORIGIN      
        1 gttcgaagct ccggcccggc caggacgaga cgtggtcgct tcaggtccgg gggccgaaag
       61 cggacgtcca ggcggcggag atggtcgcct ccctttacga cgcctcgctc gatcagttct
      121 atccgcacgg cttcggcggg atttccgcgt ttttccggaa tgacgcgacg tatctccggt
      181 cggggttcac caaccgggcg gagccgctgg ggacgttttt cgaccggctc aatccctccc
      241 gttcttatgg ggaggccgtt tacatccatt tcccccggat gatcacggaa tcgctgttcg
      301 atttcgatta cggcgaccgg gtcctgatgg cggccgaagc cgtcccggcg cccatggctg
      361 cctctcggga agccaagaac gaagtctcgg ggggcgtaat cggaggcgtc ctgggaggtt
      421 ccgccggcga aaaggaagcc gggaaggact ccggcccgcc gccgccggtg atcgaccttt
      481 tctcgatcca ggcccgggtc aatctcaacg aaaccgcgtt cttctatccc cacctcctga
      541 ccgatgccga aggaacagtc accctcgagt tcaagatgcc cgaggccttg accgaatggc
      601 gctttctcgg cttcgcccac acgaccggcc tgcgaagcgg atcgatcgag gcggcggccg
      661 tcacccagaa ggagctgatg gttcagccga atccgccccg cttcctccgg gagggcgatg
      721 tcctggaatt cacggttaag gtcaccaaca tgaccgagaa ggaagcggcc ggcgcggtcg
      781 agctccgctt cttcgacccg cgcaacgacg cgccgctcga cggggcgctg gccaacgcga
      841 atccgaagct ttcgatggcg attccggcca agcagtcgcg gacgttttcc tggccgatca
      901 cggttccgga cggcgcggag accgtcggct tccgggcggt cgcggccgcg gccgggtttt
      961 ccgacggcga ggaggggatg gtccccgttc tcagccgcag cctgatggtc cgggaatcga
     1021 tcccgctgtg gatcagcggc cgcggggaaa agcgcttccg gttcgacaag ctcgcgcagt
     1081 cggccggctc gacgactctc cggtccctcg gcttgacggt gcagatgtct tcaaatccgg
     1141 cctggtacgc ggtccaggcg ctgccttact tgatggaata tccctacgag tgctccgagc
     1201 aggtcttcaa ccgtttgtat gccaacgccc tggcccgaaa gatcgccggc tccgatcccc
     1261 gcatccgccg ggtcttcgag ctgtggaagg gaaccccggc cctccagtcc aacctggaga
     1321 aaaaccagga gctgaaaagc gtccttctcg aggagtctcc ctgggtggtc gaggccaaga
     1381 acgaatcgca ggccaagcgg aacatcggac tcctgtttga cgagaacacg acggccccgg
     1441 ccttgaagcg ggccgttcaa aagctggtcg acatgcagct ccccgacggg tcgtggccct
     1501 ggttcccggg cggcccgggc aacgactaca tcaccctcta catcgtgacc ggcttcggcc
     1561 ggctcgagca tctgggcgtc ccgggaatcg cccgggaggc ggttctccgg gcggtcggcc
     1621 ggctggattc ctggatccgg gaggtccatg aacggatcct ggcggacaag acggaaaagc
     1681 tcaacaacct cagcccgtcg atcgctcttt atctttatgc ccggagcttt ttcctggatg
     1741 gatgcccgat tccggacgct tcgaggaaag ccctcgacta tttcctgggg cagggagcgg
     1801 ctttctggct ccagctcgac agccgccaga gccaagccca tctcgccctg ggcctcaacc
     1861 ggttcgggga cgcggagacc gctcgaaaaa tcacccggtc gctcaaggag cggagcaagg
     1921 tggacgagga gctcggccgg tactgggccg agggagaggc gtcctggtgg tggcgccggg
     1981 cgcccatcga aacccaggcg atgatgatcg aggccttcga cgaggtggcc ggtgacgcgg
     2041 ccgcagtcga ggaatgccgg atctggctgc tcaaacagaa gcaaacccag gattggaaga
     2101 cgaccaaggc cacggccgac gccgtgtacg gactgatcct tcggggggcg gacctgctgg
     2161 cctcggatga aatcgtcgag gtgatcctgg gcggccgcaa ggtcgagccg gagaaggtgg
     2221 aggcggggac gggcttctat gaaaagcgct tttccgcggc cgagatccgg ccggaaatgg
     2281 gggaaatcga ggtccgcaag acggacccgg gcatcgcctg gggcggcgtc cactggcagt
     2341 acctggagga tatttccaag atcacccccc acgagcggaa cccgctccgg ttgaaaaaaa
     2401 gcctttttaa aaagacgttc accaagaaag ggccggaaat cgagccggtc cgcggccccc
     2461 tggcggtcgg cgacacgctg gtcgtccgcc tcgagctgcg gaccgaccgg gacatggagt
     2521 acgttcacct gagagatcac cgcggctccg ggctcgagcc ggtcaacgtc ctctcgacgt
     2581 accggttccg ggacggcctg gcctactacg aggcgacgaa ggacgcggcg gctcatttct
     2641 tcatcgatga tctcccgaaa ggcgcctatg tcttcgaata tgagctgcgg gtccagctcc
     2701 gcggccgcta tcagaacggg atggcccaca tcgaatgcat gtatgcgccc gagttcaaca
     2761 gccactctga aagccgggtt ttggaggtgc gctgacttgc ggccggggat tcgtgatgtg
     2821 atatcatagg gcgccgcggg ctcctccggg gagccgccgc gattccgcgc ctgaaagggc
     2881 aaaggagaaa ctggatggac atcgtcatga ccacggaatc cgacgttgcc gttttctcga
     2941 tcatcggcca tctggacgcc gtttcctcgc cggagctgga gggccggctc ggaacgtggt
     3001 tcgaggagcc gggaacgcgc ctggtcctgg atctcaacgg tctcgaatac atcagcagcg
     3061 ccggcctgag ggtcatcctc tcggcggcga aaaagatgaa ggcccggaac gggaagctcg
     3121 gcttgtcccg gcttcagccc cgggtgaggg aagtcttcca ggtgtccggc ttttcctcca
     3181 tcatcccggt cttcgagacc ctgcccgcgg cgcttgacgc cgtccggtga gggcggcccg
     3241 gaaaccgacc gccatggaac gcctccggcg gcccgcccgc atggagtttc ttcatgagct
     3301 tcttgaattc atcgacgccg gggccaaacg gctcggattc gacgaatcgg cccgcggccg
     3361 gatccgcctg gcttccgagg aagccctggt gaacgtcatc ctccacgctt atgccggcgc
     3421 ggacggagag gtcgaggccg gcttgtcggg aggggaggat cgttcgctcc ggatcgagat
     3481 ccgcgaccga ggggccgcct tcgatccctt ggccgaagcc gagccggatc ttgaagcggg
     3541 cctcgccgac cgcaagatcg gcgggttggg catctttttc atccgggaag cggccgaccg
     3601 ggcggcttat cgccgcgagg gggaggagaa catcctgacc ctttatttta tccggacgta
     3661 aggggagagg ccttcgccgt ttcgggttcc ttttttttcg aaaccagggt caccagcacg
     3721 gccagggtca tggcgatgat gaacgtcgag gcccgggcgg tgaccgtttt ctccagcccg
     3781 gccagcatcc aaacgacggt ggagacgaga cccgaggcga gagcggtcac gacgccggcc
     3841 gcgttgaagc ggtcccagta gaacgacaga agcacggccg gggcgaacga gcagccgatt
     3901 cccgcccagg cgtagctgac gatggaataa atgagatctt tcgtcgtcaa ggccaggacc
     3961 agccccaaga cgccgacggc gacggtcgaa aggcgtgaaa tcaagagcag ggtgcgctcg
     4021 cgggcccggc cttttttcag gatgccctta tagacgtcct cgctgatgga cgtggcggcc
     4081 acgagaagca tgctctcggc cgtggagatc atggccgcga ccgccccggc caggagcagg
     4141 gccgccagcc agcccgggaa caggttgttc aggatgaagg gaagaatcat ctcggaatcg
     4201 gccaccgcct tggggccgaa gaggacatgg gccccgaggc cgatgatgac cacgccgctg
     4261 taggcgatga tcgtccagag gacggcgatg ttccggccta agcggacgtt tttttcgtcc
     4321 ttcatgccca tgaaccgggc gttgagctgg ggctggccgc cgaggtaccc gaaaaaccag
     4381 gcgaggttgt tgaagatcat caggccggcg gcgaatcccg ccagcccccc ggtcagcgag
     4441 ccgaatccgg gtccggccgc ggcgaaggct tgagccgggg agacggggga ggcgaggatc
     4501 ttgacgagga ggatgacggg ggtgacgacg agggtggtca gcatcaggat ggactggaac
     4561 gtgtccacgg ccacgacgct gacgaatccc ccgaacgtcg cgtatcccag gatgacgacg
     4621 gccgagatga tcatccccca gaacgggctg aagccgaagg tctgttggat cgtcttgccc
     4681 gcgccggcga actgggcccc gacgtagaaa acgaaaaaga agacgatgat gccggtcgac
     4741 agaacccgga tcaagttggc ctttgaggcg aacttggccg cgaagtactc gggcatggtc
     4801 atggccttgt aagcttcggc ctcggcccgg aatttgcggg ccaggacgat ccaggccgtg
     4861 gagatgccga gcaggcagcc gacgcccgtc cagatgccgg acaggccggt ggcataaatg
     4921 aatcccgtca agccgagcag cagccaggcc gactctcccg tggcccgctc ggagagggcc
     4981 agcatccagc cgggcagctt cttgccgccg aggacgaagt cggactggga tttcgttttc
     5041 cgggcctccc agaggccgag gcacaaggtc aaggcgaagt aaaggacaaa gacgatgatc
     5101 atgaccggcg atcctttcgg taacgtttcc ggcgaaggtt cgcggccaaa aatattacag
     5161 aatggagatg tttttcaagt cccgccgccg tccgcctcac ggcccggctt acatgtattt
     5221 gccgctgatg taattcgaga gctgttcgat cagggcgtct ttctccgaga tgatcgcctt
     5281 cacgacgtcg ccgatcgaga ccaggccggt gaacttctcg ccttcgcaga ccggcaggtg
     5341 gcggacgtgc ttggcggtca tgagaaccat gcattcttcc acggtgttgt ccgggccgac
     5401 atggaacatc ttgccggccg gggtcatgat ttccgaaaca agagtctcct cggaggattt
     5461 cccgaacagg atgattttcc gggcgtaatc ccgctcggtg atgatgcctt ccatccggcc
     5521 cttgtcgtcc atgaccatca gggccccgat ccgcttctcg gccatgagct tcaaagcctc
     5581 gaacaccttg gcatggggcg agatggacca gactttggaa cccttggcgg cgaggatgtc
     5641 cttgacgttt ttcatgattc tctccgtaac ggctgtttga tctcaatgta tagggaaatc
     5701 cggacgattt caagggccga ttttccggcc cggttcaggc cggcgggcga cttgacaaat
     5761 gaagaaatca aattaaatta ctagatatat cgcgttaacg tcttgatgtg tcgaagggag
     5821 aaagggaaat gcagcatcgg ccgcattacg ggaattgggg acggggcgag cggatctttc
     5881 acaaggggga tttcaagtac ctgatcctcg atttattaaa ggagaaaccc cgccacggat
     5941 acgaaatcat ccgcgagctt gaaaaccagt tcaacggctt ctatacgccc agcccgggag
     6001 ccgtataccc gactctgcag tatctggagg agatggggca tgtcacgtcc cagcccctgg
     6061 acgggaaaaa gatctacacc atcacggaat ccggtctcgc cttcctggcc gaaaaagcca
     6121 aggagacgtc cgagatcaag gcccagatga agaactggtg gggatgctac ggggcggagt
     6181 ttcgggatga aatgcgggat gtcatgcgga cgctcggcga tctcggcaaa acgatcggcc
     6241 agaaagcccg gcaagccgga gtcgagaaga tgccccaagt caagcaagcc ctggccgagg
     6301 cggtcaagga gatcgagaag atattcaagt cctgatccgc cggcgggcgc gtcgggcttg
     6361 gtcctccgtc gtgactcctc tcttgagcgg gacgctccgg gaccgccttg acagaccgcc
     6421 ggggcggttt gacatccgtt ccggggggag gctatgctgt ttgcctcctt cggcgcgagg
     6481 catccgccgg aaatgaaagg caggaggaag agatgaacaa gcgaactgcg gcggcgtttc
     6541 tcattgcggc gatggtcttt ttatcggcgg gagcggccgc ggcggaaggg gaggccgacc
     6601 gcgacggccg gatgcaatgg tggcgggagg cccggttcgg ccttttcatt cattgggggc
     6661 tttacgccgt gccggccggg gagtggcggg gcaaaacgga ttatggcgag tggatccgcg
     6721 ataccgcccg gattcccctt caggtctatg atcagttcgt cggccggttc aatccgaggg
     6781 aattcgacgc cgaggcctgg gtccgggcgg ccaaggacgc ggggatgaaa tatatcgtca
     6841 tcacctcgaa acatcatgac ggattcgccc tcttcgattc gaaacagacc gatttcgaca
     6901 tcttggccac gccgttcggc cgggacgtcc tccgggagct ggcctcggcc tgccggagcc
     6961 aggggctgaa gctctgcttt tattattcga tcatggactg gcatcacccg gattacctgc
     7021 cccggcggcc gtgggaggaa agaatccggc cggtcccggg gggcgacttt gatcgatacg
     7081 tcgcctacat gaaggcccag ctccgggagc tgttgaccca gtacggggac atcggcgtct
     7141 tgtggttcga cggcgaatgg gagaacacgt ggaacatcga ccgcgggcgg gatctctacc
     7201 ggtacgtccg gtcgcttcag ccgtcgatca tcgtcaacaa ccgggtcggg tcggaccgtt
     7261 ccgggatgga ggggttctcg gcggcccggg acgccccggg tgatttttcc actccggagc
     7321 agcaggttcc cggccggggg ctccccggcg tcgattggga gacctgcctg accatgaacg
     7381 acaactgggg gtacaaccgg gccgatcaga actggaaatc ggtccggacc ttgatccgga
     7441 cgctggtcga cgtcgtctcc aagggcggca atctcctctt gaatgtcggc ccgaccgccg
     7501 agggagtgtt tcccggcccg agcatggagc gcctggcggc catcggccgc tggatgaggg
     7561 tcaacggcga gtccctctac gggaccacgg ccggcccgtt cagcgacctg cgctggggcc
     7621 ggtcgaccca gaagaaggat ggggaaaaca ccctgatctt tctccatgtt ttcgattggc
     7681 cggagaacgg ctggctcaat cttcctccga tcgccgagga actccggacg gcgtatgtcc
     7741 tttccgcccc ggccaagccc ctgccggtcg agttccggga attcgggccg cggatccgcg
     7801 tcccggccca ggctccggac gccgatgaca cggttatcgt tctcgtcacc ggggcggaga
     7861 ccaaatccta cgccccgccc cggatcacgg cggccgccaa gtcttttctc gacgtcctcg
     7921 aggtgaccat cacgggcgat ccggccttca ccgggatccg gtacacgctc gacgggtcgg
     7981 aaccgaccct cgagtccccg ttggcggccg gccccgtgcg cttgaccgag acaacggtcg
     8041 tctcggcccg cggcttcatc aagggcgttc cggtcaccgg gacggctcaa gaaacgttca
     8101 cgaagaccgc cctccgcccg cccgacgacc cgggcggcgg cctcaagcag ggcttgagct
     8161 tcgcttatta cgagggcgag tggacggccc ttcctcaatt cgcggggatg aatccgatcc
     8221 gccgcgggga ggctctccac atcgacctct cccaaaaacg gcggcacgaa aactacggat
     8281 tcgagttcaa gggattgatc cgggctccgg aggacgggct ttacgctttt gccttggaat
     8341 ccgacgacgg cagccggctc tggatcggcg aaaccctcgt tgtcgacaac gacggcgtcc
     8401 acccttcggc cgagcgcggc ggggctatcg ccttgaaagc cggattccat cggatcacca
     8461 tccggtattt caaccggacg gggggagagg agctcaaggt gttttggaag gccccgtccg
     8521 gaccgaaaca gctgatcccg gaccggatgc tattccggag ggaagcccgc ttctgaggat
     8581 ttccccgatc gcccgctcga cggggacggg ctctcagcgc tcctctttgg gaatcaccac
     8641 ccacatgatc aggtaaacga gaaggccggg gaacgcggcg ctgaggatcg aaccgacgac
     8701 gtagacgacg cggaggagcg agacatcgag gccgaagtaa tcggccaatc ctccgatcac
     8761 tcccccgatc atccggttgg tcgtcgaccg gcgcaagact tttctggatt cgttcatttt
     8821 tcgactcctc cgtgatcaag aaccctcgcc cggagagacc gtccgccggc caagcgtccg
     8881 cgcctccctc aggccgccgg cgccttcttt ttcccgaacc gcaacttcat cgtcaggatc
     8941 ttgtcggtcg agaagatccg ggcggcgtag atcaccagga cgatgaaaac gagaagcatg
     9001 tagcccagcc cggccgcgat catcccgtaa tggccgaggt agatattctg cgaaacgagg
     9061 aacgggtggg agaagggaat ggccaggacg aggatcttga ccgggagcga gagcgtgtcg
     9121 atgtcggcga acagggagat gaaatagggg atcatgacca tgaacatcag gggcatgatc
     9181 atgttctggg cgctccggaa gtcctcggcc aggacgccca ggatcatggc catggccagg
     9241 gcgaccagga tggccaggaa gagggagagg ccgagaatga gatagccggt cgtgtcgaac
     9301 gtcaggccga gcttctgcat cagggcggtt ccgcttgccc gcgccccggc cgcggcgact
     9361 tcctcgccca tcccgccgat gaagcccttg aaggcgaaca tgtagactcc cgccgagatc
     9421 aggccgacga gtccggccgc gaacatcttg gcggtgatga tggaggtccg tttgatgggc
     9481 accgtcagga gcgtctccag ggtcttgttc tgcttctcca tggccacggc cgagatgacc
     9541 atctgggacg aatagatgac gatcatcatc aggacgatgg ggatgagcat cgactgctgg
     9601 gaaagcattc cggcgatcaa gccggccgac ccctcggccg tccggtcccg gacgacgacg
     9661 aagtcccggc tcttgatcgg gcgcttgagg ctgaccgggt cggccccagg gagcttcgcg
     9721 gccaggaagt cgttggagag ggtttcgttc aaggccgaga tgacgctttg gacgacgacc
     9781 tggccccggg ctccgatcag ggaaaagctc cgcatgaagg aataggtctc gatctcgctg
     9841 ggcttaagct cggccagcga atcgccgaat ccggccggga cgaccagaag cagcttcgac
     9901 tcgccggcct tcgccgaggc cagggcttcg tccttgccct tgcctgtctg gtcgacgatc
     9961 ttgaaccggg ccgattccag gcctttcagc agagcccggg aaccctccga gccgtcctgg
    10021 tcgaggaccg agatcgtctg gattccgacg gccttctgga cttccttctt gctgatctgc
    10081 ccgatggcgt tgaagaggag gacggtgaag gccagggaga tgacgagctg cttggtcacc
    10141 agctccttga cttccttctg cagcaggttt ttaaagcgtt tcatcggagg gaactcctga
    10201 agacctcttc caggttcttc gcggcatgct tggccttgag ctcgtcgggc gtcccggtgt
    10261 cg
//
LOCUS       DAOOXH010000091         9880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_7440_length_9880_cov_7.439898, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000091 DAOOXH010000000
VERSION     DAOOXH010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 9880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9880
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7440_length_9880_cov_7.439898"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PKV64_09485"
     CDS             complement(<1..621)
                     /locus_tag="PKV64_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008853654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPN17344.1"
                     /translation="MDESTRDRTNAPGQCNFCHRTPGEVRKLIAGPGGVFICDLCVEA
                     AHSLVKSASAADVPDKDDLPESLPTPAEIKAGLDEYIIGQELAKKKIAVAVYNHYKRI
                     RSPRGASDVEIAKSNILLIGPTGTGKTLMAETLARIISVPFAIADATTLTEAGYVGED
                     VENIVLKLLQAAGGNIARAQKGMIYIDEIDKISRKSESPSITRDVSG"
     gene            complement(681..1277)
                     /locus_tag="PKV64_09490"
     CDS             complement(681..1277)
                     /locus_tag="PKV64_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPN17345.1"
                     /translation="MTLIPFVVEQDGRSERAYDIYSRLLKDGIIFLGSEITDDTANQV
                     IAQLLFLEAENPQREISLYINSPGGSITAGLAIYDTMQFVGNDVSTICVGQAASMAAV
                     LLAAGTPGKRFALPNARILLHQPFGGTQGQASDIAIQAKEILRMRETLYQILVKHTRQ
                     SKSKIQSDIERDFIMTAPQAKEYGLLDGIYVSRGAAKK"
     gene            complement(1296..2573)
                     /gene="tig"
                     /locus_tag="PKV64_09495"
     CDS             complement(1296..2573)
                     /gene="tig"
                     /locus_tag="PKV64_09495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPN17346.1"
                     /translation="MADPQDDLKCTIETLGPVSRELDVEVSAAEAARVHEEMAASFAS
                     RAKLPGFRPGKAPLDVVLQKFGPDIEHEVIDQLIPESLHRVLEKNAIRAVGIPSVRDV
                     SYRAGDPIRYTAVVETWPEFDLPPYKKLKLKKRDESVSDEDVAKTIEDLRLKHAEYLP
                     VENRGIQAGDFVILQIQGRDLKTKRLMPVEKISVVAGHEGNDPAVEAHLSGLRTGEET
                     AFEHAYPADDPRKKLAGKTIAFTMKILSVRETVTPAVDDELAKRMGEESLDVLRKKVR
                     TELEALKRRDARRQSSEEAIQAVIDQASIPLPESAVEEETAVVLKNLAAQLPRRGLSR
                     EAVESIRAEARRQAEQNLKEHLVIRKIAQAEGFQVTEEDVDAEIKAIAEANNLPLARM
                     MDTFREEDRREGLKGTLLSRRTVDFLISQSIME"
     gene            complement(2617..2699)
                     /locus_tag="PKV64_09500"
     tRNA            complement(2617..2699)
                     /locus_tag="PKV64_09500"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2664..2666),aa:Leu,seq:tag)
     gene            complement(2834..4675)
                     /locus_tag="PKV64_09505"
     CDS             complement(2834..4675)
                     /locus_tag="PKV64_09505"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN17347.1"
                     /translation="MKAKKLSIFALSLALLLPAASLFAQSELAAKLLVQSGNGVLMLT
                     VFDKDKKEIGRGAAVVLSETIAVTTYHLVDKAADVKAMNAKKKKVNVKGLLAVDKVLD
                     LALLQIDGKIDVLPTGGALVPDMTVVAVGSDKLGDMAGSEGKIRKLFDLAPDVAIADS
                     TLALKDEFDGGAVLDEAGIIVGLQNVSDLRLRYIVPVAALKALRPGTLIPFKNRTPED
                     FRESVEGAWLAGRVYMWLDVGRSAVQNLEKVTKAQPNNVEAWKLLAGVYESQRDYQKA
                     AGAFEQLTKIDPRNAEAFAGLGRIQSRMRKSYEDTSNLGKAVENLEAALRLDPSMTSV
                     YEDLGNCYEGLREWAKAGDAYDKFLATKPENPLQAYKMLGNVRREAGQFEEAAAAYAE
                     VVKLIPLDVYQYYQLAQMLEKAKKYEDAETAYIKTGEISNEPGKYFPNILAMYDGAGM
                     TDKAIAAGQRMVEIAPDNEQSHYNLGLQYQKIGKNAEAIAAFQKAVEIKPTFEYGWFQ
                     IGSCYYAMKNYSKAITAFQKNAELVPDQIYGWMYLGMSQMQIKQFGAALESMKKAVDL
                     KPDNTDALFNLGVIYLNLRDRTSALEIVKKLQALDQAKAAKLKSYIK"
     gene            5007..6182
                     /locus_tag="PKV64_09510"
     CDS             5007..6182
                     /locus_tag="PKV64_09510"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HPN17348.1"
                     /translation="MANPRRTATVLIAAALTSAALFIFKINGDMVDFNVNYAAGDRLV
                     HGETLYRASDEHYQFKYAPFCAMIYAPLSLLPPAAARAIWYALVLTSTAGMILLTIRL
                     IRGNDAAVEGGLGPRVSALLAGLVLAKFILREIQLGQINAIITVLMLLVIFNLYKDET
                     NKPAVGRAAASGLLWGLATAMKPYSLIFLPYFLLKRRWNILWPALLFLGLSLIAPAVY
                     YGFPGNLAVHVEWITTLMRSTSTLLDTQDNVSLLAGLTKWTGDPGLAQMGFVFFLLAL
                     VLLVFFFIRRRREKTGSAVPEGALLLLLVPLISPLGWDYTFLSSILAVAIVFRHLHRF
                     PKIGRLFLILNFTVIGLSLFDLLGRKAYAVFMSASVLTLNFLVIVVAMVSLRWTRKI"
     gene            6164..6679
                     /locus_tag="PKV64_09515"
     CDS             6164..6679
                     /locus_tag="PKV64_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HPN17349.1"
                     /translation="MDEENLILRRRGLRIYVLTSAGTAAWLAALFAAPFFQSLGWRGA
                     RFLYACFAPVCHQDPGRTIFLWGAPLAVCARCLGIYLGFGLGLILYPFRRSFCSVQLP
                     RLRSFIFASLPIAADFAANILGLWSTPHLPRLFTGVIWGSILPFYFITGLTDLSARRI
                     SVKNPGAAGQA"
     gene            complement(6648..7379)
                     /locus_tag="PKV64_09520"
     CDS             complement(6648..7379)
                     /locus_tag="PKV64_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17350.1"
                     /translation="MNISDDRLKRAYRSYVDGLCRGTRKDCPPPADLAGFFSAGVPRL
                     FKAGILDHVAGCGSCAREFEIFLAMDRSAREFAEKSAILLQAEKKDRKPRSSRGFIVL
                     GRWLFAGVGFAAALGLFLFLARKPGDGPAAAAEVFRSGRASGLSLNYPRGTVEGQSAL
                     VFNWRPVERSAGETYIVSLFDDSLKRIWISPVITETNLVLPEEIGRSLESRRRYYWMV
                     KRSSLSPASESDLVPFTLVRRRLDF"
     gene            complement(7366..7911)
                     /locus_tag="PKV64_09525"
     CDS             complement(7366..7911)
                     /locus_tag="PKV64_09525"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPN17351.1"
                     /translation="MSWDSNKERELERIFTLFSGFMRARAYKHQVGRLGVDVEDIIQE
                     TRIKIWKLLSNETKINHYASYIKKIVDTSVIDYFRKFKREEGIYFYEKNRRIGENVST
                     FEADHLYGGMDLKDIVGKAVDGLIPSRRLVVRLYLLNMTVEEIALHFQWTVSKTKNLL
                     YRGLKDLRRILKEKSIDYEHL"
     gene            complement(7908..>9880)
                     /locus_tag="PKV64_09530"
     CDS             complement(7908..>9880)
                     /locus_tag="PKV64_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HPN17352.1"
                     /translation="GKRLADKNKDREAASNIYIGHGVALKHMGREEEALSFFRDAISL
                     AEEIKAGRILWDAHLETADILKKKGFFLEALGHYQDAIDVIERSRSTIDDEDLKTSYL
                     RANRRLDVYHGAIDLLSLMHEANPDARHLEEVFQYMERSKARGFLDSLEVGRVSAARP
                     GDPGIETRTAEIRARLSGLTRKRYYEPASAGQKAALDLELRSVEDEYQRLLREVRSRD
                     PLYTSLRFPEILTLRSAQKRILDRGTVVLMYAVGKEFAYGLALREEGATLFRLPPVAA
                     LRDLVSRHLKSLTDREHPDPEAGRELFKAVLAPGLDRPFKRTVIVPDDFLHYLPFETL
                     CPEGAASAAGIAGDVFYAPSVSSLAEIRKRAERRPRAKTEWLGIAASGGNVPDIPPLI
                     FAEKEVEDVARFYPSGKRLVLKGDEAKEKTLKALRLGDYRVIHFAAHALIDEQKPVRS
                     AVVLSSDPDSSEDGLLQAREIFDLRLNADLVVLSACRSAAGRLVRGEGLVGLSRSFLF
                     AGASAVLVSLWPVDDEATGHLMSRFHFHLRRGRTAAAALRLAKADMASSSGFSHPYYW
                     SGFVVSGSAGRRLYGNRRERLAAAGALAVLAAAAGIILTGRNRRRGRPTRPVDRKKNA
                     GVRIVSEGKSAAREASSKTDGFWRGGGRKASP"
BASE COUNT         1764 a         3059 c         2882 g         2175 t
ORIGIN      
        1 ccccgaaacg tcccgggtga tggacgggct ctcgctcttg cggctgatct tgtcgatctc
       61 gtcgatgtag atcatgccct tctgagcccg ggcgatgttg cccccggcgg cctggaggag
      121 cttgagaacg atgttttcca catcctcgcc gacatacccg gcctcggtca aggtcgtcgc
      181 gtcggcgatg gcgaagggaa cggatatgat ccgggccagg gtttcggcca tcagggtctt
      241 gcctgttccc gtgggtccga tgagcagaat gttgcttttg gcgatctcga cgtccgaggc
      301 gccccgcgga ctccggatcc ttttgtaatg gttgtacacg gccacggcga tttttttctt
      361 ggccagctcc tggccgatga tgtattcatc caagccggct ttaatttccg cgggcgtcgg
      421 caaggattcg gggaggtcgt ccttgtccgg gacgtccgcg gccgacgcgc ttttgacaag
      481 ggaatgggcc gcttccacgc aaagatcgca gatgaacact cctcccgggc cggcgatgag
      541 cttgcggacc tcgcccggcg ttcggtggca gaagttgcat tgcccgggcg cgttggtccg
      601 gtcccgcgtg gattcgtcca tgatcgtctc tcgttatccg gctcggattc cagaccggct
      661 ctcaaggccg gtccgccgga tcatttcttg gcggctcccc gcgagacata aatcccgtcg
      721 aggaggccgt attccttggc ctggggcgcc gtcatgatga agtcccgttc gatgtcggac
      781 tgaatcttcg atttcgactg gcgcgtatgc ttcaccagga tctgatagag cgtttccctc
      841 atccggagga tttccttggc ttggatggcg atgtcggagg cttgcccctg ggtccctccg
      901 aaaggctgat gcaggaggat ccgggcgttc ggcaaggcga accgttttcc cggcgttccg
      961 gccgccagga ggacggcagc catcgaggcc gcctggccga cgcagatcgt cgacacatca
     1021 ttgccgacga attgcatcgt gtcgtagatc gccaagccgg cggtgatgct gcctccgggc
     1081 gaattgatat acagcgagat ctcccgctgg gggttttccg cctcgaggaa gagaagctgg
     1141 gcgatgactt ggttggctgt gtcgtccgtg atttcgcttc cgagaaagat aatcccatcc
     1201 ttgagaaggc gcgaatagat gtcgtaggcc ctctccgaac ggccgtcctg ttcaacgaca
     1261 aaggggatga gcgtcatggc ggaaaaagcg atggtttatt ccattataga ctgggagatt
     1321 aaaaagtcaa cggtccgccg ggaaagcagc gtccccttca atccctcccg acggtcttcc
     1381 tcccggaagg tgtccatcat cctcgccagg ggaaggttgt tggcctcggc gatggccttg
     1441 atttccgcat cgacgtcctc ctccgtcacc tggaacccct cggcctgggc gatcttgcgg
     1501 atgacgagat gctccttgag attctgctcg gcctgccgcc gggcttcagc ccgaatcgat
     1561 tccacggctt cgcggctgag cccgcggcgg ggaagctggg ccgcgaggtt cttgagaacg
     1621 accgccgtct cctcctcgac ggcgctttcg ggaagaggga tggaggcttg gtcgatgacc
     1681 gcctggatcg cctcctcgga ggattgccgt cgggcgtccc gccgtttcaa ggcctccagc
     1741 tccgtccgga ctttcttccg gagaacgtcg agcgattcct cccccatccg cttggccagc
     1801 tcgtcgtcga ccgcgggcgt gacggtttcc cggaccgaaa ggattttcat cgtgaaggcg
     1861 atcgtcttgc cggccagctt cttgcgggga tcatcggccg ggtacgcgtg ttcgaaggcg
     1921 gtctcctccc ctgtccggag accggagaga tgggcctcga cagccggatc gttgccctcg
     1981 tgaccggcga cgaccgagat tttttccacg ggcatcagcc tcttggtttt gaggtcccgg
     2041 ccttggatct ggaggatgac gaaatcgccg gcttggatcc cccggttttc gaccgggagg
     2101 tactcggcgt gtttgaggcg cagatcctcg atcgtcttgg cgacatcctc gtccgaaacg
     2161 ctttcgtccc gtttcttcag cttcagcttc ttataaggag gaaggtcgaa ctcgggccag
     2221 gtttcgacga ccgccgtgta ccggatcggg tcgcccgccc ggtaggatac gtcccggacg
     2281 gaggggatgc ccacggcccg gatcgcgttc ttttccagaa cccggtgaag cgactcggga
     2341 atcagttgat caatcacttc gtgctcgatg tccgggccga atttttgaag aacgacgtcc
     2401 aagggagcct tgcccggccg gaagccgggg agtttcgccc gggaggcgaa cgaagccgcc
     2461 atttcctcgt ggacgcgggc cgcttcagcc gcggagactt cgacgtccag ctcccggctg
     2521 acggggccga gcgtctcaat ggtgcatttt aaatcgtctt gggggtcggc catggatgct
     2581 tcctttatcg ccggcggaaa ccggcgcgtt gatccgtggg cagagcggga ctcgaacccg
     2641 cactccctct caggaactag gtcctaagcc tagcgcgtct gccgttccgc cacctgccca
     2701 tatgtgattt cgcccggcaa gcatgtttcc ctaaaaattc cgccgggggt tgactcgggg
     2761 gaaacggaag gagagtttta aaaataccac accgtctccc ctttgtcaat tcaatccggg
     2821 ccggccggtc cgcttacttg atataagatt tcagcttggc cgctttggct tggtcgagcg
     2881 cctggagctt cttgacgatt tccaaggcgc tcgtccggtc cctcaggttg agataaatga
     2941 cgccgagatt gaaaagggcg tccgtattat cgggcttgag gtcgaccgct tttttcatcg
     3001 actccagggc cgccccgaac tgcttgatct gcatctggct catgccgaga tacatccagc
     3061 cgtagatctg gtcggggacc agttcggcat ttttctggaa ggccgtgatc gccttggagt
     3121 aatttttcat ggcgtaataa caggagccga tctggaacca gccgtattcg aacgtcggct
     3181 tgatctcgac cgccttctga aaggcggcga tcgcctcggc gtttttcccg atcttctggt
     3241 actgcaggcc caggttgtag tgagactgct cgttgtcggg cgctatttca accattctct
     3301 gcccggcggc gatcgcttta tcggtcattc cggctccgtc atacatggct agaatattgg
     3361 ggaagtactt ccccggttca ttgctgattt cgccggtctt gatgtaggcc gtttcagcat
     3421 cttcatattt cttggctttt tccagcattt gggccagttg atagtactgg tagacgtcga
     3481 gggggatcag cttgacgacc tcggcatagg ccgccgcggc ctcttcgaat tggcccgcct
     3541 cgcggcggac gttgccgagc atcttgtagg cttggagagg attttctggt ttcgtcgcca
     3601 ggaatttgtc ataggcgtcg cccgccttgg cccattcccg aagaccctca tagcagttgc
     3661 cgaggtcctc gtaaaccgac gtcatcgacg gatcgaggcg gagggccgct tccaagttct
     3721 ccacggcctt gcccaggttc gacgtgtcct cgtagctttt ccgcatccgg gactgaatgc
     3781 ggcccaggcc ggcgaaggct tccgcgttcc ggggatcgat cttcgtgagc tgctcaaacg
     3841 cgccggccgc tttttggtaa tccctctggc tttcataaac cccggccagc aatttccacg
     3901 cctcgacatt gttcggctgg gctttggtca ccttttcgag gttttgaacg gccgagcgac
     3961 cgacgtccag ccacatgtac acccggccgg ccagccaggc gccctcgacc gactcccgga
     4021 aatcctccgg cgtccggttc ttgaagggaa tgagggttcc gggccgcagg gccttgaggg
     4081 ccgcgaccgg gacgatgtag cggagacgca gatcggaaac gttctggagg ccgacgataa
     4141 tccccgcctc gtccaggacc gccccaccgt cgaactcatc cttcagggcg agggtggaat
     4201 cggcgatcgc gacgtcgggg gcgaggtcaa acagcttgcg gatttttccc tccgaaccgg
     4261 ccatgtcccc gagcttatcc gagccgacgg cgacgacggt catgtctgga accagggcgc
     4321 cgccggtcgg caggacgtcg atcttgccgt cgatctgaag aagggccagg tcgaggactt
     4381 tgtccacggc caagagcccc ttcacgttca cttttttctt cttggcgttc atggccttga
     4441 cgtcggccgc tttatcgacg agatgatacg tcgtgaccgc gatggtttcg gagaggacga
     4501 cggccgcgcc gcgtccgatt tccttcttgt ctttatcgaa aacggtgagc atcaggacgc
     4561 cgtttccgct ttgaacgagg agcttcgccg ccagctcgct ctgggcgaag agcgaagccg
     4621 ccggaagaag aagggcgagg ctcaacgcga aaatggagag ttttttggct ttcatttcgc
     4681 ctcctgttta tgtttggacg agaatgattg aaaaatgtag gagatatttt gacacgaccc
     4741 tccgcttctg tcaaatatgc tggaggcttc gccccgcgaa acggccgaag cccgctccgg
     4801 gcgagatcca ggctcacggc gcggatcggg gcggcccgcc cggcggaatt tgagccgaaa
     4861 aaccaagacg gtccccatca tcgcgtcccg ggaaatgatc gtccgaggcc cgggcaaaag
     4921 gcgcgttcgt gaacgatccc ggattcaacg ccgttgacag aaaacgacgc gtcgggtagc
     4981 ctgaggaacg gagaattcaa gaacccatgg cgaatccccg ccgaaccgcc accgtcctca
     5041 tcgccgccgc gttgacctcg gccgctcttt tcattttcaa gatcaacggc gacatggtgg
     5101 atttcaacgt gaattacgcc gccggcgacc ggctcgttca cggcgagacg ctctaccgcg
     5161 cctccgacga gcactatcag ttcaagtacg ccccgttctg cgccatgatc tacgctccct
     5221 tgtccctcct gcctccggcg gcggcccggg cgatctggta cgccctggtg ctgacttcga
     5281 cggccggtat gatcctcctg acgatccgct tgatccgagg gaacgacgcc gcggtcgaag
     5341 gcggcttggg acctcgagtc tcggcccttc tggccggtct cgttctggcg aaattcattc
     5401 tccgggagat tcagctcggc cagatcaacg ccatcatcac cgtcttgatg ctgctcgtga
     5461 ttttcaacct ctataaagac gagacgaaca agcccgccgt cggccgggcg gccgcctcag
     5521 ggctgctttg gggcttggcc acggccatga agccgtattc cctgattttc ctgccgtact
     5581 ttcttctgaa gcgccgttgg aacattctct ggccggccct tctttttctg ggtctgtccc
     5641 tgatcgctcc ggccgtttat tatggttttc ccggcaacct ggccgtccac gtggaatgga
     5701 tcacgaccct gatgcgctcg acatcgacct tgctcgatac ccaggacaac gtttcgctcc
     5761 tggccgggtt gaccaaatgg accggcgatc cgggcctcgc ccaaatggga ttcgtcttct
     5821 tccttctggc cttggtcctc ctcgtttttt tcttcatccg ccggcgccgc gaaaagacag
     5881 ggtcggcggt tcccgagggg gctctgctgc ttctcctcgt tcccttgatc tcccctctcg
     5941 gctgggatta caccttctta tcgtccatcc tggccgtggc gatcgtcttc cggcatcttc
     6001 accggtttcc aaaaatcggc cgcctctttc tgattctgaa tttcacggtc atcggcctgt
     6061 cgctcttcga tcttctcggc cggaaagcct atgccgtgtt catgtcggcc tccgtcctga
     6121 cgctgaactt tctggtcatc gtcgtggcga tggtttccct gcgatggacg aggaaaatct
     6181 gatcctccgc cggcggggtc ttcggatcta tgtcctgacc tccgccggga cggccgcttg
     6241 gctggccgcc ctcttcgccg ccccgttctt ccaaagcctc gggtggcgcg gcgcccgttt
     6301 cctctacgcc tgtttcgccc ccgtctgtca tcaggatccc ggtcgaacga tcttcctttg
     6361 gggagccccc ttggccgtct gcgcccgctg cctcggcatc tacctgggat tcgggctcgg
     6421 cttgattctc tatcccttcc ggagaagctt ttgctccgtc cagcttcccc gcctccggtc
     6481 ttttattttc gcgtccctgc cgatcgcggc cgatttcgcc gccaacattc tggggctctg
     6541 gtcgacgccc catctccccc gcctgttcac gggggtgatt tgggggtcga tactcccctt
     6601 ttatttcatc accgggctga cggacctgtc ggcccggcgg atctccgtta aaaatccagg
     6661 cgccgccgga caagcgtgaa cggcacgaga tccgattcag acgccggcga cagcgagctc
     6721 cgcttgacca tccagtagta acggcggcgg gattccaggg agcgcccgat ttcttcgggg
     6781 aggacaaggt tcgtttccgt aatcaccgga ctgatccaga tcctcttgag ggagtcgtcg
     6841 aaaagactga cgatgtaggt ttcccccgcg cttctctcca cgggtcgcca gttgaagaca
     6901 agggcggatt gcccctcgac cgtcccccgg ggataattca aggagagacc cgacgctcgg
     6961 cccgaccgga aaacctccgc ggcggccgcc ggcccgtcgc ccggtttccg tgctaaaaag
     7021 agaaaaaggc ctaaagcggc ggcaaagccc actccggcga acagccatcg gcccagaacg
     7081 atgaatcccc ggctcgaccg cggctttcga tccttttttt ccgcctggag gagaatcgcc
     7141 gatttttccg cgaattcccg ggcggaacga tccatggcta ggaatatttc gaactcccgg
     7201 gcgcaggatc cgcacccggc gacatgatcc aggatccccg ctttgaacag gcgggggacg
     7261 ccggccgaaa aaaaaccggc caaatcggcc ggcggagggc agtcttttct cgtcccgcgg
     7321 caaagcccgt ccacatagct ccggtaagcc cttttcaagc ggtcgtcaga gatgttcata
     7381 gtcgatgctt ttttccttta agattcgcct cagatccttc aggccgcggt aaagcaaatt
     7441 cttcgttttg gaaacggtcc attggaaatg aagagcgatt tcctcgaccg tcatgttcag
     7501 gagatagagc cggacgacca agcgccgact cgggatcaag ccgtcgacgg ccttgccgac
     7561 gatgtccttg aggtccattc ccccgtaaag gtgatccgct tcgaatgtcg agacattctc
     7621 tccgattctc cgattttttt cataaaaata gatcccttcc tcccgcttga atttccggaa
     7681 ataatcgatg accgacgtat ccacgatctt ctttatataa gacgcgtaat gattgatttt
     7741 cgtctcatta gacaggagct tccaaatctt gatccgggtt tcctggatga tgtcctccac
     7801 gtccacgccg agccgaccga cttggtgctt gtaggcgcgg gcccgcatga acccggaaaa
     7861 caaggtgaat attctctcca attcccgctc tttgttcgaa tcccagctca cggcgacgcc
     7921 tttcgccccc ctcccctcca aaagccgtcg gttttggaag aggcttcccg ggcggccgat
     7981 tttccctccg atacgatgcg gacgccggcg ttctttttcc ggtcgaccgg ccgggtcggt
     8041 cgtccgcgcc ggcgattccg ccccgtgaga ataatcccgg cggcggccgc cagcacagcc
     8101 agcgctccgg ccgcggcgag ccgctctctc cggtttccgt aaagacgtcg gccggcggat
     8161 cccgagacga cgaacccgct ccaataatag ggatgcgaaa accctgagga cgaagccatg
     8221 tccgccttcg ccaagcgaag ggcggcggcg gccgtccggc cgcgccgcag atgaaaatga
     8281 aaccggctca tcaagtgtcc ggtcgcctca tcatcgacgg gccacaagct caccagcacg
     8341 gccgacgcgc cggcgaacag aaacgaccgg cttaatccca caaggccctc gccccggacc
     8401 agccggccgg ccgcgctgcg gcaagccgaa aggacgacca ggtcggcgtt caagcggaga
     8461 tcgaagatct cccgcgcctg gaggagtccg tcctcggagg aatccggatc cgaggaaagg
     8521 acgaccgccg aacggaccgg cttttgctcg tcgatcaagg cgtgggccgc gaaatggatg
     8581 acccggtaat ctccaagccg gagggctttc agcgtctttt ccttggcttc atcccccttc
     8641 aagacgaggc gtttcccgga tggatagaaa cgagcgacat cctccacttc tttttcggcg
     8701 aaaatcagcg gcggaatatc cggaacgttt ccgcccgagg cggcgatccc cagccattcc
     8761 gtcttcgccc gcggtcgtcg ctcggccctt ttccggatct cggccagcga agaaaccgac
     8821 ggcgcataaa acacgtcgcc cgcgattccg gcggcggatg cagcgccttc gggacaaagc
     8881 gtctcaaagg gcaagtaatg aagaaaatcg tcgggaacga tcaccgtccg cttgaacggc
     8941 cggtctaggc ccggggcaag aacggctttg aaaagctccc ggccggcctc gggatcggga
     9001 tgttcccggt ccgtgagaga tttcaagtga cgggaaacaa gatcccggag cgcggcaacc
     9061 gggggaagac ggaaaagggt cgccccctcc tcccggaggg ccaagccgta cgcgaactcc
     9121 tttccgacgg catacatcaa gacgaccgtc ccgcggtcca ggattctttt ttgcgcgctc
     9181 ctcagcgtca gaatctccgg aaagcgaagg ctcgtataga ggggatcgcg ggaccggacc
     9241 tcgcggagga gacgttgata ttcgtcctcg acggaccgca gttcaagatc gagggcggct
     9301 ttttgcccgg cggacgcagg ttcataatac cgctttcggg ttaagccgga aagccgggcg
     9361 cggatctcgg cggtccgggt ttcgatcccg ggatccccgg gtcgggcggc cgaaacgcgg
     9421 ccgacctcca agctgtccaa aaaacctctc gccttggatc gttccatata ttgaaagacc
     9481 tcctcgagat gacgggcgtc cggatttgcc tcatgcatca aggagagaag gtcgatggct
     9541 ccatgataaa cgtccaatct tcggttcgcc cgcaaataac ttgttttgag gtcctcatcg
     9601 tcgatggtcg aacggctccg ctcgatgacg tcgatcgcgt cctggtaatg gccgagagcc
     9661 tccaaaaaga agcctttttt ctttaagatg tcggccgttt ccaagtgggc atcccacagg
     9721 atgcggccgg ccttgatttc ttcggccagg gagatcgcgt cccggaagaa gcttaaagct
     9781 tcctcctccc ggcccatatg tttcaaggcg acgccatgcc caatataaat gttacttgcg
     9841 gcttcccgat ccttgttttt atcagcaaga cgcttgcctt
//
LOCUS       DAOOXH010000092         9623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_7732_length_9623_cov_6.362772, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000092 DAOOXH010000000
VERSION     DAOOXH010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 9623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9623
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7732_length_9623_cov_6.362772"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PKV64_09535"
     CDS             <1..187
                     /locus_tag="PKV64_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HPN17353.1"
                     /translation="ESFLGKTIAELQLRSLYNVMVIAVKDVLTDQVTTLPPAHFVIKD
                     GQVLVLVGKEADIEKLR"
     gene            247..711
                     /locus_tag="PKV64_09540"
     CDS             247..711
                     /locus_tag="PKV64_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPN17354.1"
                     /translation="MRPTDYLDSQAILLDLPASDKGRAFSLIADRLAESRLITDRGRF
                     LDDVLKREQQGGTAVGRGLAIPHARTRALERIVMAMARLRPAVDFGAEDGEPVRLVFL
                     LGAPEDKAGEYLKVLGKLSRLLKENGLRKELLKAATPEAVLELLEAAESQLS"
     gene            794..1783
                     /locus_tag="PKV64_09545"
     CDS             794..1783
                     /locus_tag="PKV64_09545"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPN17355.1"
                     /translation="MKALVTGATGFIGGHLVEALIARRDEAFVLIRPGGRGAGSLPPA
                     AALVRGDYGDPDSLARAVSGMDVVFHAGGLITSLDEAAYEAANVRPTRALLSACASSA
                     PALKRFVYVSSISATGPSPAGGRLDESAPLRPVSGYGRSKAAAEEAVRSFSGRVPWTI
                     LRPPNVLGPRQKELFNSISLIGKRIKPLMGDRRPRTSIAAVWDVVRALLLLADHPAAV
                     GRTYFVTSGRPYAWREITDAVAAELGVRRVYLPVPYPAQKAVGAVSELAARIRGREPL
                     LTREAVDAAWKYSWIYDDSRIRNELGFVPEYDLAGAIRRTVAWYAERGLARIR"
     gene            1780..2739
                     /locus_tag="PKV64_09550"
     CDS             1780..2739
                     /locus_tag="PKV64_09550"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPN17356.1"
                     /translation="MNGTDGRGRLRGSAIARLHGRRIRVFGFPLQITDALVLGGLLVQ
                     AALFLLFFRRLDRPLFLAAKNAGLAALYLLCVFLLSKIKARVPFFLLRTASVQLLFAY
                     LYSATHPLQLMLMPWQDRPVLAAEQAFFGIQPTLWMERFVSPPLTEWLMFSYVIYLVI
                     YPLVAGLVFFKHGEGALEDYLFTLGIVNLACDLGFILFPVASPLWYMPEAYAVPLQGG
                     LFTFLGDFIRAHAHQAGGSIPSPHCAVATVMWLTARRYVRPWFHILGPVILSLYVSTV
                     YCRYHYVTDSVAGLALGIIAILAAPGMVRAWNRKAGRRSQEAA"
     gene            2736..3737
                     /locus_tag="PKV64_09555"
     CDS             2736..3737
                     /locus_tag="PKV64_09555"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPN17357.1"
                     /translation="MNAVFITGANGFIGSNLCRHFLAAGWKVSGLVRPASDLHLLDGL
                     GLDLVRGDLCDSSSVRIPEDAAHVIHAASVVSDVADADACRSHIFDLTRNLLELLRRR
                     TVPLRRLVYVSSALTLGYGATDISEDRPGVPAEFLPYTRFKILCEQYLLDEHARRGLP
                     VVILRPADVVGPGDRTTSARLFREAERGLPLIVGRGRRRFGLTFVGNLCRAAEAALVR
                     DGVCGRAYAVTNGVLPTWGEYLGAVAAGFGKKQRIYVPAWAAFAAARAMEAVHKIRPS
                     YDPKLTTYRIRRVTTDTTYDISRTRDELGYVPDDDFSRQVAETLAWYREERAHGHIR"
     gene            3721..4428
                     /locus_tag="PKV64_09560"
     CDS             3721..4428
                     /locus_tag="PKV64_09560"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPN17358.1"
                     /translation="MAISVETSAWPGILLAYEAVALTLLQTVFSRRRFLKNAAGGDLP
                     AVRSYFLAVFTFGLAVPVTAVFSLAADPVAVFRALGLSGGNVRLGWILAAAGIPTALA
                     AGAVGAGDPRLRDFYPFAKDALAGRRAFIGYELGYFLFYYTAWEFIFRGVLFFPVAAS
                     AGLLPALAVSTIASTLFHIGHPETELYAALGAGFVFGLAAWGAGSFLYVIPIHAAVGI
                     ATDAFIYRRAARERSSR"
     gene            4425..5432
                     /locus_tag="PKV64_09565"
     CDS             4425..5432
                     /locus_tag="PKV64_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPN17359.1"
                     /translation="MRLLVTGATGFVGAVLVPRLLEKYGPAAVSAFVLPGDRIPDSWA
                     GRGVRIHEGDIADAAAVRAAVAGRTQVVHLAGVISYWKGDRERLFRVNRDGARVVGEA
                     CLETGVQRLVHLSSVGAVGFHKNGRPADEKTPYNWPDSILYMASKHAGEEAVEKLIRT
                     RGLRAVILNPASIMGPGDHDPRTPHNRLYGMISRSRISASFSGGLAVVDVRDLVDLIL
                     DALEGKGRDGERYLAVGANLSYAEVIRLIGRACGRGTRPFRLPAAVIAAGGGLAEAAG
                     SLSRKRPLLTFAYGRLSGWRAYYDNAKSVREFGHAYRPVAQTVADGWEYYRRIHRKEN
                     P"
     gene            5429..6775
                     /locus_tag="PKV64_09570"
     CDS             5429..6775
                     /locus_tag="PKV64_09570"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPN17360.1"
                     /translation="MNQASAHSLPRVLGLGDVIALVVGGIIGSGIFLVPKDMAAAVGH
                     PLLILAVWVAGGVLSMFGALSFGELGASMPEAGGVYVYLREAYGSAVAFLFGWTLFLV
                     IDTGAIATLAVAFSSKYLPHFFAISPAGQKAVSILFILFLTVMNYIGVRRGADLQNVL
                     TIFKLVALTAVCGAIFLFAEGDTAHFVRSGGAEPSGGLLGAFGVALVASLWAYKGWEA
                     ATYSAGEMRNPQRTLPWGILISCGICVVFYVVAQLAYLYVFPAAEIALSDRIAAEAMN
                     AAVGGLGASIISFIILFSILGAANQNFVCSPRVYYAMAKDGLLFPQLAALHPRFLTPH
                     NSILALAAWSIVLSLSGTFEQLFTYVVFGQWLFFGLTVAAVIVLRRKRPDLPRPYRTW
                     GYPVTPVVFILASLYIAVSALISQPGKALAGLGIILLGLPFYFFGRKRAKKRVASP"
     gene            6991..7632
                     /locus_tag="PKV64_09575"
     CDS             6991..7632
                     /locus_tag="PKV64_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17361.1"
                     /translation="MKKNPGFMITVAAVMALMMIGAGCASKKHLLKIEQQGAEIQQSQ
                     ERIAELQRNNEILTGTLNETKASLVKAEESRSEADKQITALNSDVSALKSRNEDLSKS
                     MEALNKENEGKVRRLNGQIYSLRKQQTEKDEQIAAKDGDIASLKSLQASLEGTVSARD
                     QQISRLNEEKASEAAASAKTISGKNLLITILGVLLALAIIVAVFALFKRKKRS"
     assembly_gap    7643..7742
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7765..8562)
                     /locus_tag="PKV64_09580"
     CDS             complement(7765..8562)
                     /locus_tag="PKV64_09580"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HPN17362.1"
                     /translation="MNARAEAAQNGAGRLVLAVIPHAGLLVPEEIRPEDLAENWSELT
                     RNIDWHTGELYDFRDILADGRVVFPYCSLVLEANRDPRNLEAAVPLKDVWGRPVYRPG
                     REPGLDLRRRMAARYLDPFHARIGEAIASGAGLVLEGHATVSARGVSGNQIDLMNIQL
                     SPGEEPLRFCPDALIEGYAEELRKRLPGVNVTINASGYLSVYGHIGARHSIDSPDRAG
                     RRAPLILQETNQALYLRPDGAPDLERLNRLRRAFAESLRTITTAFSA"
     gene            complement(8559..>9623)
                     /locus_tag="PKV64_09585"
     CDS             complement(8559..>9623)
                     /locus_tag="PKV64_09585"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPN17363.1"
                     /translation="ERTPMSVIPSNGIKVCFIAPSTSLVPSDQPVLERSVRILTESFE
                     TSGIYLSPYLYSSDSRIDHVTAAASQRAEEFKKVIREFDLIVSVAGGTGAEDIVPRID
                     RHDLRVIRKRRPLFLGYSDFTFLLCEIFARAGVPGVLFPSLSLGRGHTGRILSLLSGR
                     RVSFRGSFWLTPPPDGPLAGIPIGGNLTTFVNFLNREKPPRLPWLEHILFLEDLSIDV
                     EDLHRLLAALRRHRVFSRVRALVVGSLCLDVNTPEGRTFQDSTLKFLTAFLNEILKTR
                     RNAGRPLPILVAEDFGHLVGPREPAVPIGGTATLFPDGHLEFKLPGKALGPFRKHPNP
                     AEPAKDVLPVLSPVPEAAPK"
BASE COUNT         1526 a         3111 c         3025 g         1861 t
ORIGIN      
        1 cgagagcttt ctggggaaga ccatcgccga gctgcagctt cgctcgctct ataacgtcat
       61 ggtcatcgcc gtcaaggacg tgctgaccga ccaggtcacc acccttccgc cggcccactt
      121 cgtgatcaag gacggccagg tgctggtcct ggtcggcaag gaagccgaca tcgagaagct
      181 ccgctgaggc ggctggcctt ttcccccgct ttgatgtatg gttgagcgat aaaacgtcaa
      241 aatcccatgc gtccgaccga ctatctcgat tcacaagcga tcctgctcga tctgcccgcc
      301 tccgacaaag gaagggcttt ctccctcatc gccgaccggc tggccgaaag ccgtttgatc
      361 accgaccgcg gccggttcct ggacgatgtg ctcaagcgcg agcagcaggg cggcacggcc
      421 gtcggccggg gattggccat cccccacgcc cgcacgaggg ccctggagcg gatcgtcatg
      481 gccatggccc ggctgcggcc cgcggtcgac ttcggcgccg aagacggcga gccggtccgc
      541 ctggtcttcc tcctcggagc cccggaggac aaggccggcg aatacctcaa ggtcttgggg
      601 aagctctcca ggctgctgaa agaaaacggc ctgcgcaagg agcttctgaa agccgccacg
      661 ccggaggcgg ttttggagct tctggaagcg gccgaatccc agttgtccta agagcggatc
      721 cggacagaca aggttttccc aaatcgtccg actctgataa aatcgctttc atcatgcgtt
      781 gtcaggcgtc cggatgaaag cgctcgtcac cggggcgacg ggattcatcg gcgggcatct
      841 tgtcgaggcg ctgatcgccc gccgcgatga agcgttcgtc ctgatccgcc ccggcggccg
      901 gggggcggga tcgctccctc ccgcggccgc tctcgtccgg ggcgattacg gcgatccgga
      961 ctccctggcc cgggccgttt ccggcatgga cgtggttttt cacgccggcg gcctgatcac
     1021 ctccctggac gaggcggctt atgaagcggc caacgtccgg ccgacccggg cgcttctttc
     1081 cgcctgcgct tcctccgccc cggctttaaa gcggttcgtc tatgtctcga gcatcagcgc
     1141 gaccgggcct tcgccggccg gcggacgtct cgacgaatcc gctcctctcc ggcccgtctc
     1201 cggttatggc cgaagcaagg ccgcggccga ggaagccgtc cggtcgtttt ccggccgcgt
     1261 cccctggacg atccttcggc cgcccaacgt cctcggcccc aggcagaagg agctgttcaa
     1321 ctccatttcg ctgatcggga agagaatcaa gccgctgatg ggggaccggc ggccgcggac
     1381 gagcatcgcc gccgtttggg acgtcgtccg ggcgctcctt cttctggccg atcatccggc
     1441 ggccgtcggc cggacctatt tcgtcacgtc cggccggccg tatgcctggc gggaaatcac
     1501 ggatgcggtc gcggccgagc tgggcgtgcg gcgcgtctat cttccggtgc cttatccggc
     1561 gcagaaggcc gtcggggccg tctccgagct ggctgcccgg atccgcggcc gagagcctct
     1621 tctgacccgg gaagcggtcg acgccgcctg gaaatactcc tggatttacg acgattcccg
     1681 catccgaaac gagctgggct tcgtcccgga atacgacctg gccggagcga tccggcggac
     1741 cgtggcctgg tatgcggagc ggggattggc gaggatccga tgaatggaac ggacggccgc
     1801 ggccgccttc ggggaagcgc gatcgcccgg ctccacggcc ggcggatccg cgttttcggc
     1861 ttcccccttc aaatcaccga cgccctcgtc ctcggcggcc tgctcgtcca ggcggccttg
     1921 ttccttttat ttttccgccg cctcgaccgg ccccttttcc tggcggcgaa aaacgccggc
     1981 ctggccgcct tgtatcttct ttgcgttttc ctgctttcga aaatcaaggc gagagtccct
     2041 ttctttctcc ttcgcaccgc ctcggtccag cttctgttcg cgtatctcta cagcgccact
     2101 catccgcttc agttgatgct gatgccgtgg caggaccggc ccgtcctggc cgccgagcag
     2161 gctttcttcg ggatccagcc gaccctttgg atggagcggt tcgtctcgcc tccgctgacc
     2221 gaatggctga tgttttccta cgtgatttac ctggttattt accccctggt ggccgggctc
     2281 gtctttttca agcacggcga gggagccctg gaggattacc ttttcacctt gggaatcgtc
     2341 aacctggcct gcgatctcgg atttatcctc tttcccgtgg ccagcccgct ctggtacatg
     2401 cccgaggcct acgccgtccc gcttcaaggc ggcctgttca cgtttctcgg cgatttcatc
     2461 cgggcccacg cccatcaggc cggcggatcg atccccagtc cgcattgcgc cgtggccacg
     2521 gtcatgtggc tgaccgcccg acgctacgtc cgcccctggt tccatatcct cggccccgtc
     2581 atcctcagcc tctacgtctc gaccgtttat tgccgctacc attatgtcac cgactccgtc
     2641 gctggcctcg ccctcgggat tattgctata ctggcggcgc cggggatggt ccgggcctgg
     2701 aaccgaaagg ccggccgtcg atcccaggaa gccgcatgaa cgccgttttc atcaccgggg
     2761 ccaatggatt catcggttcc aacctctgcc ggcattttct agcggccggc tggaaggtct
     2821 ccggattggt ccgcccggcc tccgatctcc atcttctcga cgggctcggc ctcgacctgg
     2881 tgcggggcga cctttgcgat tcgtcttccg tccggatccc ggaggacgcg gcccatgtca
     2941 tccacgccgc ctcggtcgtt tcggacgtgg ccgatgcgga cgcctgccgc tcccatatct
     3001 tcgacctgac gcgcaatctc ctcgagctcc ttcgaaggcg gacggttcct ctccgccggc
     3061 tggtctatgt ctcctcggcc ctgaccctcg gctacggggc gacggacatc tccgaggatc
     3121 ggccgggcgt tcccgcggaa tttcttccct acacccgctt caagatcctc tgcgagcagt
     3181 atctcctcga cgaacacgcc cggcgcgggc ttcccgtcgt cattcttcgt ccggccgatg
     3241 tcgtcggccc cggcgaccgg acgacctcgg cccgcttgtt ccgcgaggcg gagcgggggc
     3301 tgccgctcat cgtcggccgc ggccgccgtc gtttcggcct cacgttcgtc ggcaatcttt
     3361 gccgggcggc cgaggcggcc ctcgtccgcg acggggtctg cggccgggct tacgcggtga
     3421 ccaacggcgt cctgccgacg tggggcgaat atctcggggc cgtggccgcc ggattcggaa
     3481 agaagcagag aatttatgtc cctgcttggg cggctttcgc cgcggcccgg gcgatggaag
     3541 ccgttcataa aatccgtccg tcctacgacc cgaagctgac gacttaccgg atccggcggg
     3601 tgacgaccga cacgacgtac gacatttccc ggacgcggga cgagttgggt tatgtccctg
     3661 acgatgattt cagccgccaa gtcgcggaaa ccctggcctg gtatcgggag gagagagccc
     3721 atggccatat ccgttgagac gagcgcctgg ccgggaattc tcctggccta cgaggccgtg
     3781 gccctgaccc ttcttcagac ggttttcagc cggagacggt ttttgaaaaa cgccgccggc
     3841 ggcgacctgc cggcggtccg gagctacttt ctcgcggttt tcaccttcgg cctggccgtc
     3901 ccggtgaccg cggtgttcag cctggccgcc gatcccgtcg cggtcttccg cgccttgggc
     3961 ttgagcggcg ggaacgtccg gctgggatgg attctggccg ccgccgggat cccgacggct
     4021 ctggccgccg gagcggtcgg agccggcgat ccgcggctcc gcgatttcta tcccttcgcc
     4081 aaagacgcct tggcgggccg gcgggcgttc atcggctacg agctgggtta cttccttttc
     4141 tattacaccg cctgggaatt catcttccgc ggcgtcctgt ttttccccgt cgcggcctca
     4201 gccggcctgc ttcccgccct ggccgtgtcg acgatcgcct cgaccctctt tcatatcggc
     4261 catccggaaa cggagcttta cgccgccctc ggggcggggt tcgtcttcgg cctggccgcc
     4321 tggggagccg gatcttttct ttacgtgatc ccgatccacg cggccgtcgg gatcgccacc
     4381 gacgccttca tctaccggag agcggcccgg gagcgctcct cccggtgaga ctcctcgtca
     4441 ccggggcgac cggattcgtc ggagcggtcc tcgttccgcg cctgttggaa aaatacggtc
     4501 cggcggcggt ctcggccttt gttcttcccg gggaccggat cccggactcc tgggccggac
     4561 ggggcgtccg gatccacgag ggggacatcg ccgacgccgc ggccgtccgg gccgcggtcg
     4621 cgggccggac gcaggtcgtc cacctggccg gggtgatttc ctactggaag ggcgaccggg
     4681 aaaggctgtt ccgggtcaat cgcgacgggg ctcgcgtcgt cggcgaggcc tgcctggaga
     4741 cgggcgtcca acgcctggtt cacctgtcct cggtgggagc ggtcggattt cacaagaacg
     4801 gccggccggc cgacgagaag acgccgtaca attggccgga ttcgatcctt tacatggctt
     4861 ccaagcacgc cggggaggag gccgtcgaga agcttatccg gacgcgcggc ctccgggcgg
     4921 tcatcctcaa tcccgcctcg atcatgggac cgggcgacca tgatccaagg acgccgcaca
     4981 accgcctcta cgggatgatc tcgcgcagcc ggatcagcgc ctcgttttcg ggcggactgg
     5041 ccgtcgtcga cgtccgggat ctcgtcgatt tgatactcga cgccctggag gggaagggcc
     5101 gggacggcga gcgctacctg gccgtcgggg ctaatctctc ctatgccgag gtcatccggc
     5161 tcatcggccg ggcttgcggc cggggaaccc gcccctttcg gcttcccgcc gccgtgatcg
     5221 cggccggcgg gggcctggcc gaggcggccg gttcgctgtc gcggaaacgg ccgcttctga
     5281 ccttcgccta cggccggctg agcggctggc gcgcttacta tgacaatgcc aagagcgtcc
     5341 gcgagttcgg acacgcgtat cggcccgtcg cccaaaccgt cgccgacggc tgggaatatt
     5401 accggcggat ccaccggaaa gaaaatccat gaatcaagcg tcggctcata gccttccccg
     5461 cgtcctgggc cttggggacg tcatcgccct cgtcgtcggc ggcatcatcg gttcggggat
     5521 ctttctcgtc cccaaggaca tggccgcggc cgtcggccac cccctcctga tcctggcggt
     5581 ctgggtcgcc gggggcgtcc tcagcatgtt cggcgccctg tcgttcggcg agctgggcgc
     5641 ctcgatgccg gaagccggcg gcgtttatgt ctacctccgc gaggcctacg gctcggcggt
     5701 cgccttcctc ttcggctgga ccctgttttt ggtcatcgac acgggggcga tcgccacctt
     5761 ggccgtcgcc ttctcctcaa agtacctgcc tcatttcttc gcgatctcgc cggccggaca
     5821 gaaggcggtc agcatcctct tcatcctctt tttgacggtg atgaactata tcggcgtccg
     5881 ccggggggcc gatttgcaga acgtcctgac gatcttcaag ctcgttgctt taaccgccgt
     5941 ctgcggagcc atcttcctgt tcgccgaggg ggatacggct catttcgtcc gttccggcgg
     6001 agcggagcct tccggcggcc ttctcggggc gttcggtgtc gccctggtgg cctcgctctg
     6061 ggcctacaag ggctgggagg cggcgacgta cagcgccggg gagatgagga atccccagag
     6121 aacgcttccc tggggcatct tgatcagctg cgggatttgc gtcgtttttt acgtcgtcgc
     6181 ccagctggcc tatctctacg tcttcccggc ggctgaaatc gccctttccg accggatcgc
     6241 ggcagaggcg atgaacgccg ccgtcggcgg gctcggcgcc tcgatcatct cgttcatcat
     6301 cttgttttcc atcctcgggg cggccaacca gaatttcgtc tgctcgccgc gcgtctatta
     6361 tgccatggcc aaggacggtc tcctgttccc gcagctggcc gccctccacc cgcggttcct
     6421 gaccccccac aactccatcc tcgccctggc ggcctggagc atcgtcctga gcctgagcgg
     6481 gacgttcgag cagctgttca cctacgtcgt cttcggccag tggctctttt tcggcctgac
     6541 ggtggccgcg gtcatcgttc tccggcggaa gcgtcccgac ctgccgcgtc cctaccggac
     6601 ctggggatac ccggtcacgc cggtcgtctt catcctggcc tccctttaca tcgccgtcag
     6661 cgccctgatc agccagcccg gcaaagccct ggccggcctg ggaatcattc tcctcggcct
     6721 gccgttttat ttcttcggcc ggaagcgggc gaaaaaacga gttgcctccc cgtaaaaggg
     6781 gctatcccct caatctccgg gttccagcgg ttttcagctt caaatccgcc gtcaaggcga
     6841 tgtcaccggg cggagctcga cgcgcggcgc ctctccgccc agcggatcga tcggtcttcc
     6901 gctcgaggga gtctcttcat cgaaaacctt gatttttttt cttgaatggc ttaatttcct
     6961 tttgtatcga cgggaaagga gagattgacc atgaaaaaaa atcccggctt catgatcacc
     7021 gtcgcggcgg tcatggcatt gatgatgatc ggcgcaggat gtgcctcgaa aaagcacctc
     7081 ctcaagatcg agcagcaggg agcggagatc cagcagtccc aggagaggat cgccgagctt
     7141 caaagaaaca atgagatcct gaccgggact ctgaacgaga cgaaagcctc tctagtaaaa
     7201 gccgaggaaa gccggtcgga ggcggacaag cagatcaccg ccctgaattc agatgtttcg
     7261 gccttgaagt cgcggaacga agacctgtcg aaatcgatgg aagccctcaa caaggaaaac
     7321 gagggcaaag tccgccgttt gaacggacag atttactccc tgcggaagca gcagacggaa
     7381 aaagatgaac agatcgcggc gaaggacgga gatatcgctt ccctcaagag tctccaagcg
     7441 agcctcgagg ggaccgtatc cgcccgcgat cagcagattt cccggctgaa cgaggaaaaa
     7501 gcatccgagg ccgcggcctc ggcgaaaacg atctccggga aaaacctgct gatcacgatc
     7561 ttgggcgttc ttctggccct ggcgatcatc gtcgccgttt tcgctctttt caagcggaaa
     7621 aagcggtcgt aatcgtccgg agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnatcgtcgc cgttttcgct cttttcaagc ggaaaaagcg gtcgtaatcg tccggaggga
     7801 ctcggcgaag gcccggcgca accggttgag gcgttcgaga tcgggcgcgc cgtccgggcg
     7861 gagatagaga gcctggttgg tttcctgaag gatgagcggg gccctgcgtc cggcccgatc
     7921 cggcgaatcg atcgagtgtc gggcgccgat gtgtccgtaa acggagaggt agccggacgc
     7981 gttgatggtg acgttcactc cgggaaggcg cttccgcagc tcttcggcat agccttcgat
     8041 cagggcgtcg ggacagaacc gaagcggctc ttctccgggc gagagctgga tgttcatcaa
     8101 gtcgatctgg tttccggaca ccccccgggc cgagacggtc gcgtgccctt ccaggacaag
     8161 gcccgctccg ctcgcgatcg cctcgccgat tcgggcgtga aacggatcga ggtaccgggc
     8221 ggccatcctc cggcgcaggt cgagacccgg ctcccggccg ggacggtaga ccggccgtcc
     8281 ccagacatcc ttcaagggga cggccgcctc caggttacgg ggatcgcggt tggcctctaa
     8341 aaccaggctg cagtagggga agacgacccg gccgtcggcg aggatatcgc ggaaatcgta
     8401 cagctcgccg gtatgccaat cgatgttccg ggtcagctcg gaccagtttt ccgccaggtc
     8461 ttccggacgg atttcctcgg gcacgagcag gccggcgtgg ggaatgacgg cgagaacgag
     8521 ccggccggcg ccgttttggg ccgcttccgc ccgggcgttc atttgggagc cgcctcggga
     8581 acgggcgata aaacgggcag aacgtcctta gccggctcgg cgggattcgg atgtttccgg
     8641 aaaggaccca aggccttgcc cggcagcttg aattcaaggt gtccgtcggg gaatagcgtg
     8701 gccgtcccgc cgatcggcac ggccggctcc cggggtccca cgaggtggcc gaaatcctcg
     8761 gccacgagaa tcggcagcgg ccgtccggcg ttccggcggg ttttgaggat ctcgtttaaa
     8821 aacgccgtca ggaactttaa cgtgctgtct tggaaggtcc gtccttccgg agtattgaca
     8881 tccaggcaaa gggatccgac gacgagggcc cggacgcggg agaaaacgcg gtgacgccgg
     8941 agggcggcca ggaggcgatg gagatcctcg acgtcgatgc tcagatcctc aagaaacagg
     9001 atatgctcga gccacggcag gcgcggcggt ttttcccgat ttaagaaatt gacgaaggtc
     9061 gtcaggttgc cgccgatggg gattccggcc agcggcccgt ccggaggagg agtcaaccag
     9121 aaggatccgc ggaaggacac ccggcggccg gaaagcaggg ataggatccg gccggtatgg
     9181 cctcgcccca gggaaagcga aggaaagagg actcccggca cgccggcccg ggcgaagatt
     9241 tcgcacaaga ggaaggtgaa gtccgagtag cctaaaaaca gggggcgccg cttccggatg
     9301 accctcagat catggcggtc gattctcggg acgatatcct cggccccggt cccgccggcg
     9361 accgagacga tcagatcgaa ttcccggatg acttttttga attcttccgc tctctgggaa
     9421 gcggcggccg tcacgtgatc gatccggctg tcggaggagt aaagataagg gctgagatag
     9481 atccccgagg tctcgaagga ttcggtcaga atgcggacgg agcgctccaa gacgggctgg
     9541 tctgagggga ccaggctcgt cgatggagcg atgaagcaga ccttgattcc attcgagggg
     9601 atgacactca tgggggtccg ctc
//
LOCUS       DAOOXH010000093         9523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_7843_length_9523_cov_8.764892, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000093 DAOOXH010000000
VERSION     DAOOXH010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 9523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9523
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7843_length_9523_cov_8.764892"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="PKV64_09590"
     CDS             <1..1218
                     /locus_tag="PKV64_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17364.1"
                     /translation="ARILSHNKIQVFLSERDAPSQAQAYQIIKRRAQGGINFTASFNP
                     PEYNGLKYNVETGAPAMPAETEALEKEIRRLQGDYSVFPGYPRTEYIERIDLRSDYLA
                     FIQDKIDFEIIRKAGIKVAVDSLYGTSREYLDETLSENGVPVEEIHGYIDPYFGGIAP
                     SCTEENLTELRRLVRDKKCDLGIATDADGDRFGIVDHRGNYILPNLVLAVLLEYLVAV
                     KGWRGGIARSVATTHLIDRIARAYDLPVYRTKVGFKFLADLWLQNKIVFGGEETASLA
                     VARHLPEKDGIMAGLLTAEMVAASGRRLSDLIVGLFRKYGERIGLQKNIPSNPERTRN
                     LRRLIKVPPSRLAGRPVENIETIEGIKFDFKGDDWVLLRTSGTEPLIRCYAEAGTKRD
                     LRALVKAALEKLG"
     gene            1215..1520
                     /locus_tag="PKV64_09595"
     CDS             1215..1520
                     /locus_tag="PKV64_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPN17365.1"
                     /translation="MNEKKTGGLWKKIALLFLGFLLIVLLITTVFGEKGLLSIARARK
                     AQAVLLREIEALKKEKARLEREIEALKKDPESVDKEARDKLWLIKPDEKVIVKKPGK"
     gene            complement(1549..3267)
                     /gene="sppA"
                     /locus_tag="PKV64_09600"
     CDS             complement(1549..3267)
                     /gene="sppA"
                     /locus_tag="PKV64_09600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPN17366.1"
                     /translation="MKKRSYVLILILVFFFLIAATAVSFLTLSFDRTPSIPPSAYLRL
                     DLAGPIVDYPGSGFWTAFLPVGRMLSVHDIWTTLRMAKVDRRIEGVFLKIGLLECDWA
                     KCAEIREAVLDFRRSGKKAVAYLEELPEGDKEYYLATGCDRVVLHPLGWIGINGLGGF
                     VPFFKKGLDKIGIRAEFEHVEEFKTAYNQFTESGFTPAHREMMESLLQSRFDDYVAAV
                     AEARKKTPEEVRALIDRAFFQGDQARDAGLVDDVAYEDEAVSLWVRDGRRAKPVPLAV
                     YERLDPSSLGLNTGRRIALIYGQGAIHGGTGTPQTMGSDTVAGWFQAAREDASISAVV
                     FRVDSPGGSAVASDAIWREVELTRKKKPVVVSMSDVAGSGGYWVSMGADKIIAQSQTL
                     TGSIGVISGKFDLSGLAAKLGITAEKITYGEKADIFSPYRPMTSAERDLFKKQILWIY
                     DRFTAQAAEGRHMTAEAVDRVGRGRVWTGRQARELGLVDELGGLPKAIETAKTLAGID
                     AGAEVSLAVWPKRIPFLASFFGRRKTEAAAFPAGFLPEDWRSALNWAMRLNQDRVWAV
                     MPLLPE"
     gene            3534..3827
                     /locus_tag="PKV64_09605"
     CDS             3534..3827
                     /locus_tag="PKV64_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPN17367.1"
                     /translation="MNSKKRLYVGNLNYQTTEDVLRRTFAPFGEVISVNILPNRGYGF
                     VEMAASEAAEEARLKLNGLELEGRKIIVNEAKPRAERPAESRFGGERRDFRRS"
     gene            4004..4540
                     /locus_tag="PKV64_09610"
     CDS             4004..4540
                     /locus_tag="PKV64_09610"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPN17368.1"
                     /translation="MVRAVRSGDRDAFVALTRLYQQKIFILAYSFFRNKEDAVDLVQE
                     VFLRIFQKIDSFEESRNFEAWMFQVAKNLCIDHYRRSRGRRTMESGLRVEELPLADDR
                     TPDAVRASDLKAVLARSVDRLAERQRTIFLMRHVNQMGNEEIARALNISTGTVKSLHF
                     KAIRRLRALMEPHLGCST"
     gene            4633..5337
                     /locus_tag="PKV64_09615"
     CDS             4633..5337
                     /locus_tag="PKV64_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17369.1"
                     /translation="MCPDCRKEWEDAEKILQGAAALKEDVQAAMATVDWEALSERIAD
                     AALSRRRTASSPVRERPLRPRVFVLKPLFAGALGGLLIGAAVMYFALRTPPSGPARGS
                     AISASSEFIDRAELSATKRETIDYLDKSRALLLDFVQADPREAGWILQDESSARRTAG
                     LLLKKRLMNTRLDDAAMAKARNLCDQIERMFVELSLISEEISAEEAARIQRYIEEKNL
                     LLRIKLLRGELAGSEV"
     gene            5339..6211
                     /locus_tag="PKV64_09620"
     CDS             5339..6211
                     /locus_tag="PKV64_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17370.1"
                     /translation="MRKRALIPVLVLVALFSVAFPAAAQSEAEEKAFREIKLLIFDES
                     WADALSRLEAFLAGKPADSRLAPALYYRAKCLEEIGGREEEALQAYKEYLGRGDRNRS
                     LSQDAEASIIDLAMKLYQNGDRSYLPEVEERLAHPEKEVRYFAAVRLSSVKEKKAAAK
                     SIPVLKAMLAEPDEELSNRAKLGLLRVDPEALAAVPDRHADRSSSRRSERKPKIFHIQ
                     ILEALGGKAVLSLNLPFALADLALSGISMSDRALLKERGYDIDRIIRDLQTDGTILEI
                     RDEKEGKVIKIWID"
     gene            6227..6643
                     /locus_tag="PKV64_09625"
     CDS             6227..6643
                     /locus_tag="PKV64_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17371.1"
                     /translation="MNVKKNPLIALAAITVFIAGAIPAAASSSEDWRIIKRAAHRDKS
                     CSRIVRGKARRMRIVVLEGRGRGGSLRITLPFGFIEDLVRLASVRHARWRDFERDFDF
                     DRDFDFERVAAWLKRAGPGSVLEVRDGDSIVKIRLE"
     gene            6806..8326
                     /gene="hutH"
                     /locus_tag="PKV64_09630"
     CDS             6806..8326
                     /gene="hutH"
                     /locus_tag="PKV64_09630"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HPN17372.1"
                     /translation="MIEIDDHLSMEDFLRVIRDHEPVDLSVWARERLKKGRAAVEKIL
                     RGGEAVYGVNTGFGKFQNTRIEPDKLRELQRNLILSHAIGVGGPFPADVVRGILLLRA
                     QSLARGRSGVREQVVQRLLDFLNRELHPIIPSQGSVGASGDLAPLAHMALALIGEGEV
                     EYEGTVWPAADALDSAGLEPLELEAKEGLALINGTQVMCSVLALLIEDARILLSTADI
                     AAAMSVEALKGSPSPFDERITGLRPHPGAVETAENLRRLLAGSEIRDSHADCPKVQDA
                     YSLRAVPQVHGASRDALRHVAGVVEIEMNAVTDNPLIFAEDGRVISGGNFHGQPLALA
                     GDYAGIALAELANISERRIEQMLNPALSGLPAFLAEQGGLHSGLMISQYTAASLVSEN
                     KVLAHPASVDSIPTSANQEDHVSMGTIACRKARRIFENVLWVLTIELVSAAQALDFHA
                     PLRPGKGVAAVHAAIRKTMPHLARDRYLKPDLEKVRESVRNGELVRIAREAAGPLH"
     gene            8706..>9523
                     /locus_tag="PKV64_09635"
     CDS             8706..>9523
                     /locus_tag="PKV64_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17373.1"
                     /translation="MPFSATGDNRRALRRPAAGLFLTGVIFFAACYFPSKHVESVIPY
                     GIQRDKPVAAPAKVFLRDGTIILFPSGFTAGETAIHGEGRRFVPGEPAAPDLEPVEIP
                     LDRVAAMAYYDEKLTTAEIVGTVILNVSAVPLVFSSLYCLSCPKCCFGSCPTVYTEDG
                     GEFKLAAELFSFSVGRLAESDDLDFLAGSAGPPGRPFEMRLTNEALESHRINWLRPLA
                     VVHPLGTRVFPDSNNRLVLFRRFLPPLEARNSLGEDVLPAVAEADDRAYRSPQSL"
BASE COUNT         1804 a         2993 c         2989 g         1737 t
ORIGIN      
        1 gcccggatcc tgtcccacaa caagatccag gtttttctct ccgagcgcga cgcgccttcc
       61 caggcccagg cctaccaaat catcaagcgc cgggcccagg gcggcatcaa tttcaccgcc
      121 tccttcaatc cgcccgagta caacggcctc aagtacaacg tcgagacggg agctccggcc
      181 atgcccgcgg aaaccgaagc cctggagaag gaaatccggc gcctgcaagg cgattacagc
      241 gtttttccgg ggtatccgag gacggaatac atcgaacgga tcgacctgcg gagcgattac
      301 ctggcgttta tccaggacaa gatcgacttc gaaatcatcc ggaaggccgg catcaaggtg
      361 gccgtggatt cgctttacgg gacgagccgg gagtacctgg atgaaaccct gagcgaaaac
      421 ggcgtcccgg tggaggagat tcacgggtat atcgatccct atttcggcgg gatcgccccc
      481 tcctgcaccg aggaaaacct gaccgagctg cgccgactcg tccgggataa gaaatgcgac
      541 ttggggatcg cgaccgacgc cgacggggac cgcttcggca tcgtcgacca tcgggggaat
      601 tacatccttc ccaacctcgt cctggccgtc cttctcgagt atctcgtggc cgtcaagggc
      661 tggcggggcg gcatcgcccg gtccgtggcc acgacccatc tgatcgaccg gatcgcccgg
      721 gcctacgatc ttccggtcta ccggacgaag gtcggcttca agtttctggc cgatctttgg
      781 cttcagaaca aaatcgtttt cggcggcgag gaaaccgcct ccctggccgt cgcccggcac
      841 ctgccggaaa aagacggcat catggccggg ctgctgacgg ccgaaatggt cgccgcttcc
      901 ggccgccggc tgtccgacct catcgtcggc ctgttccgca agtacgggga aagaatcggc
      961 ctgcagaaaa acatcccctc caacccggaa cgaacccgca acctccgccg gctgatcaag
     1021 gttccgcctt cacggctggc cggccggccg gtggagaata tcgagacgat cgagggcatc
     1081 aagttcgatt tcaagggtga cgattgggtt ctgctcagga cctccggaac cgaacccctc
     1141 atccgctgtt acgccgaagc ggggacgaaa agagacctcc gggcgctggt caaggccgcc
     1201 ttggagaaac tcggttgaat gagaaaaaaa ccggcggttt atggaaaaaa atcgccctgc
     1261 tcttcctcgg atttctcctg atcgttctct tgatcacaac cgtcttcggg gaaaaggggc
     1321 tgctgagcat cgcccgggct cggaaagccc aagcggttct gctgcgggag atcgaggccc
     1381 tcaagaagga gaaagcccgc ctggaaaggg agatcgaagc cttgaaaaag gatccggaat
     1441 ccgtcgacaa ggaagcccgc gataagctgt ggttgatcaa gcccgacgaa aaagtcatcg
     1501 tcaagaagcc cggcaagtaa gagggggccg gggcttcccg ggggccggtt attccggaag
     1561 caggggcatc accgcccaga cccggtcctg gttgaggcgc atcgcccagt tcagggcgct
     1621 ccgccagtcc tcgggcagaa acccggccgg gaaagcggcc gcttccgttt tccgccggcc
     1681 gaagaaagaa gccaaaaagg gaatgcgctt gggccagacg gccaggctga cctcggcgcc
     1741 ggcgtcgatc ccggccagcg tcttggccgt ttcgatcgct ttcggaagtc cgccgagctc
     1801 gtccaccaat cccagttccc tggcttggcg gccggtccag acccggcccc ggccgacgcg
     1861 atcgacggct tcggccgtca tatgccggcc ctcggccgcc tgggccgtga accggtcgta
     1921 gatccagagg atttgttttt taaagaggtc gcgttccgcg gaggtcatgg gccggtacgg
     1981 cgagaagata tcggcttttt ctccgtaggt gatcttttcg gccgtgatgc cgagcttggc
     2041 ggcgaggccg gaaagatcga atttcccgga aatgaccccg atcgagccgg tcagcgtctg
     2101 cgattgggcg atgattttat ccgctcccat ggaaacccag tatccgcccg agccggccac
     2161 atcggacatg gagacgacga ccggcttctt tttccgggtc agctcgacct cgcgccagat
     2221 cgcgtccgag gcaacggccg agccgccggg gctgtcgacc cggaagacga cggcggagat
     2281 cgaggcgtcc tcgcgggcgg cttggaacca tccggcgacg gtgtccgagc ccatggtctg
     2341 gggcgttccc gttccgccgt ggatcgcgcc ctggccgtag atcaaggcga tgcgccggcc
     2401 ggtgttcagg ccgagggagg agggatcgag ccgttcgtaa accgcgaggg gaaccggttt
     2461 ggcccgccgg ccgtctctca cccaaaggga cacggcctca tcctcgtagg cgacatcgtc
     2521 gaccaggccc gcgtcccggg cctggtcgcc ctggaaaaac gcccggtcga tgagcgcccg
     2581 gacttcctcc ggagtctttt tccgggcttc ggcgaccgcc gcaacgtaat cgtcgaagcg
     2641 gctttgcagg agggactcca tcatctcgcg gtgggccgga gtgaagccgg attcggtgaa
     2701 ctggttgtac gccgtcttga attcctcgac gtgctcgaat tcagcccgga ttccaatctt
     2761 gtccagccct tttttgaaga aggggacgaa tccgcccagg ccgttgatgc cgatccagcc
     2821 gagggggtgg aggacgaccc ggtcgcagcc cgtcgccagg taatattcct tatcgccttc
     2881 ggggagttcc tccaggtagg cgacggcctt ctttccggag cgccggaagt cgaggacggc
     2941 ctcgcggatc tcggcgcact tggcccaatc gcattccaga aggccgatct tcaaaaagac
     3001 cccctcgatc cgccggtcga ccttggccat ccttagcgtt gtccagatgt catgaacgga
     3061 gagcatccgc ccgacgggaa ggaaagccgt ccagaaaccg gacccggggt aatccacgat
     3121 cggtccggcg agatcgagcc gaagataagc cgagggcggg atggacggcg tacggtcgaa
     3181 ggacagggta aggaaggaaa ccgccgtggc cgcgatcagg aagaagaaga ccaggatcag
     3241 gatcaaaacg taagagcgtt ttttcattgg cttcaccgcc gccgcggatg cggccggatc
     3301 atcttaccat gaaaaaggat cccgatgaat tcggcggcga tttccgcgtc cgttccattg
     3361 gggggccccg cccggccgga cgcgggacac cggagaatcc cgggccgcgg cctctcgtcc
     3421 gttcccccct ttcttgatga tgttcccggg cggggcgatg atccttcccg gcctccaatt
     3481 gattttccgg gatcggtgtg gaaaaatgag gccgctcgag gaggtcgcgt ttcatgaatt
     3541 ccaagaaaag gctgtacgtc ggcaacctca actatcagac gaccgaggac gttctccgcc
     3601 ggaccttcgc cccgttcggc gaggtgattt cggtgaacat tcttccgaac cggggctacg
     3661 gattcgtcga aatggccgcg tccgaagcgg ccgaggaggc ccggctgaag ctgaacggcc
     3721 tggagctgga ggggcgcaag atcatcgtca atgaggcgaa gccgcgcgcc gaacgcccgg
     3781 ccgagtcccg tttcggcggg gaacggaggg atttccgcag gtcataatca cggccgcctt
     3841 cctctttccc ggccgttccg ggggaccggg agagggatgg atcgccgaaa gaatcgcccc
     3901 ggggggagta aacttacttg ggagttgttt cgtctcatct gatgtgagcc aagaaatcct
     3961 tccgcgagcc gccggcggcg tccccccgga cgttttgacc ggcttggtcc gcgccgtccg
     4021 ctccggggac cgcgacgcct tcgtcgccct gacccggctt taccagcaga aaatcttcat
     4081 cctcgcctat tcctttttcc ggaacaagga agacgccgtc gacctcgtcc aggaagtctt
     4141 tctccggatc ttccaaaaga tcgactcgtt cgaggaatcc cgcaatttcg aagcttggat
     4201 gttccaagtg gccaagaacc tgtgcatcga ccactaccgc cggtcgcgcg gccggcggac
     4261 gatggagagc ggcctccggg tggaggagct ccccctggcc gacgaccgga cgccggacgc
     4321 cgtccgggcc tcggatttga aggcggtttt ggcccgatcg gtcgaccgct tggccgagcg
     4381 ccaacggacg atcttcctga tgcgccacgt caaccagatg ggcaacgagg aaatcgcccg
     4441 ggcgttgaat atatcgaccg gaacggtcaa atcgctccat ttcaaggcga tccggaggct
     4501 ccgcgccctg atggagccgc atttggggtg ttcgacatga agacttgccg tcgatggaga
     4561 aaggaattcg gaggagatcc gggggaattc gcccggcggg cttcgacgag cccgtggaag
     4621 gagcattcag ccatgtgtcc cgattgccgg aaagaatggg aggacgcgga aaaaatcctc
     4681 cagggcgcgg ccgccctgaa ggaggatgtc caggcggcca tggcgacggt cgactgggag
     4741 gccctttccg agcggatcgc cgacgccgcc ctctcccgga gaaggacggc ctcctcgccg
     4801 gttcgggagc gtccgctccg gccgcgggtc ttcgtcttga agccgctttt cgccggggct
     4861 ctcggcggat tgctcatcgg ggccgcggtc atgtatttcg cccttcggac cccgccgtcc
     4921 ggcccggcgc ggggctcggc gatctccgcc tccagcgaat tcatcgaccg ggccgagctg
     4981 tcggcgacca agcgggagac gatcgactat ctggacaaaa gccgggcttt gctcctcgat
     5041 ttcgtccagg ccgacccccg ggaggccgga tggattctcc aagacgaatc ctccgcccgg
     5101 cggacggccg gtcttctttt gaaaaagcgg ctcatgaaca cccgattgga cgacgcggcc
     5161 atggccaagg cccggaatct ttgcgaccag atcgaacgga tgttcgtcga gctcagcctg
     5221 atctccgagg agatctcggc cgaggaagcg gcccggatcc aaagatacat tgaagagaaa
     5281 aatctgttgc tccgcatcaa gctgctccgg ggagagcttg cgggaagcga ggtctgatat
     5341 gcgaaaacgg gcgttgatcc ccgtccttgt gttggtcgcc ctgttctccg tggcctttcc
     5401 ggccgcggcc cagagcgagg cggaggaaaa agccttccgg gaaatcaagc tgctgatttt
     5461 cgacgagagc tgggccgacg cgctctcccg cctcgaggct tttttagccg gaaaaccggc
     5521 cgattcccgc ctggctccgg cgttgtatta ccgggccaaa tgcctggagg agatcggcgg
     5581 gcgggaagaa gaagccctcc aggcttacaa ggaatatctc ggccgcggag accggaacag
     5641 gagcctgagc caggatgccg aggcgtcgat catcgatttg gcgatgaagc tttatcagaa
     5701 cggcgaccgg tcgtatcttc cggaagtgga ggagaggctg gcccatcccg aaaaagaggt
     5761 ccgctatttc gccgctgtcc ggctgagctc ggtcaaggag aaaaaagccg cggccaagag
     5821 catccccgtc ttgaaagcga tgctggccga gccggacgag gagctcagca accgggcgaa
     5881 gctcggcctg cttcgggtcg accccgaagc cttggccgcc gtccccgacc ggcatgccga
     5941 ccggtcctcc agccggcgtt cggaacgaaa gccgaagata ttccatatcc agatcctgga
     6001 agcgctgggc gggaaggccg tgctgtcgct caacctcccc ttcgccctgg ccgacctggc
     6061 cctttccgga atatcgatgt ccgatcgggc cctgctcaaa gagagaggat acgacatcga
     6121 ccggatcatc cgggatctcc aaaccgacgg aaccattctc gaaatcaggg acgagaagga
     6181 aggaaaagtc attaaaatct ggatcgattg atccgaagga gtcctcatga acgtcaagaa
     6241 aaatcccctg atcgccctag ccgcgatcac ggtttttatc gccggcgcga tcccggccgc
     6301 cgcgtcctcc agcgaagact ggcggattat caagagagcg gcccatagag acaaatcctg
     6361 ttcccggatc gtccgcggga aagcccggcg gatgcggatc gtcgtcttgg agggaagggg
     6421 gaggggcggg tccctccgga tcaccctccc gttcggtttt atcgaggatc tcgtccgctt
     6481 ggcctcggtc cggcacgccc ggtggcgcga tttcgagcgt gatttcgatt tcgaccggga
     6541 cttcgatttc gagagagtcg cggcctggct gaaaagggcg ggcccgggat ccgtgcttga
     6601 agtgcgcgac ggggattcga tcgtgaaaat ccggctcgag taaacgccgt tatttcgacg
     6661 gggcggggcg ggagaggagg gattcctctc ccgctttttt tatccctccg ttcggacggc
     6721 gccgcggatg caatcttccg gccgatcgtt tacaatgtcc cgggcgtttc ccccccggag
     6781 ggaggagcgg ccccgaggta gagacatgat tgaaatcgac gaccatctgt ccatggagga
     6841 tttccttcgg gtgatccgcg accacgagcc cgtcgacttg agcgtctggg ccagggagcg
     6901 gttgaaaaaa ggccgggccg cggtggaaaa aatcctgcgc ggcggcgagg ccgtctacgg
     6961 cgtcaacacc ggtttcggca agttccagaa cacgcgcatc gagccggaca agctccggga
     7021 gctccagcgg aacctgatcc tctcccacgc catcggcgtc ggggggccgt ttccggcgga
     7081 tgtcgtccgc ggcatcctcc tcctccgggc ccaaagcctg gcccgcggcc ggagcggcgt
     7141 ccgcgagcag gtcgtccagc ggcttctcga tttccttaac cgcgagctcc atccgatcat
     7201 cccctcccaa ggttcggtcg gggcgagcgg cgatctcgcc cccctggccc acatggccct
     7261 ggccctgatc ggcgagggag aggtcgaata cgaaggaacg gtctggccgg cggccgacgc
     7321 cctggactcg gccgggctcg agcccctcga gctcgaggcc aaggaggggc tggccctcat
     7381 caacggaacc caggtcatgt gcagcgtcct ggccctcctg atcgaggacg cccggatcct
     7441 tctttcgacg gccgatatcg ccgcggccat gagcgtggaa gccctcaagg gaagcccttc
     7501 gcctttcgac gagcggatca ccggccttcg gcctcatcca ggggccgtcg agaccgcgga
     7561 aaacctccgg cggcttctgg ctggttcgga gatccgcgat tcccacgccg attgccccaa
     7621 ggtccaagac gcctactccc tccgggccgt gccccaggtc cacggggcca gccgggacgc
     7681 cctccggcat gtggccggcg tcgtcgagat cgagatgaac gcggtcaccg acaacccgct
     7741 catcttcgcc gaggacggcc gggttatttc cggagggaat ttccacggcc agccgctggc
     7801 cctggccggc gattacgccg gcatcgccct ggccgagctg gccaatatct cggagcgccg
     7861 gatcgagcag atgctcaatc ccgcgctttc cggcctgccg gccttcctgg ccgaacaggg
     7921 agggctccac agcggcctga tgatctccca gtacacggcc gccagtctcg tctccgaaaa
     7981 caaggtcctg gcccatccgg ccagcgtgga cagcatcccg accagcgcca accaggaaga
     8041 ccatgtctcc atggggacca tcgcctgccg caaagcccgg cggatttttg aaaacgttct
     8101 ttgggttctg accatcgaac ttgtctcggc ggcccaagcc ctggacttcc acgcgccgct
     8161 tcgtccggga aaaggcgtgg ccgcggtcca cgccgcgatc cggaaaacga tgccccactt
     8221 ggcccgggac cgctacctca agcccgacct cgagaaggtc agggaatcgg tccggaacgg
     8281 ggaactggtc cggatcgccc gggaagccgc cgggccgctt cactgatctc ggattttggc
     8341 gatccgccgg cggtttttaa aagcgaatcg ccgtttgcat ttaaaacgaa tcttggggaa
     8401 ccggttgtct taaagaagga gagctgaggg gaaccgcgct cgaggccggg gaccggatga
     8461 atccctcctc cggatcaacc gcttcatcgg attgctggaa aaacagccga ggtgtgtttc
     8521 agggaacctt cccgccggag gacgatcgcc cggacgttca gattttattg atcgttgaca
     8581 acttgccgga cgctcctctt gactttgagt tatccggctt ttatcttcga attaacgagg
     8641 gaatgatccg gttgaaaaaa aagccggcca gggtttgagg tttttctcag tgcaggggag
     8701 gagaaatgcc tttttcggca accggcgata accggcgggc gttgcgccgg ccggccgcgg
     8761 gattgttcct aaccggcgtg attttcttcg cggcctgcta ttttccaagc aagcacgtcg
     8821 aatcggtgat tccctacggg attcagcgcg ataagcccgt cgccgctccg gccaaggttt
     8881 tcctgcggga cgggacgatt attctcttcc cgtcgggatt caccgccggc gaaaccgcga
     8941 tccacggcga aggccggcga tttgttcccg gggagccggc cgccccggat ctcgagccgg
     9001 tcgaaattcc attggaccgt gtcgcggcca tggcctatta tgatgaaaaa ttgacgacgg
     9061 cggaaatcgt cggaacggtc atcttgaacg tctcggccgt tcccctcgtt ttctcctccc
     9121 tctattgctt gtcttgtccc aagtgctgtt tcggatcgtg tccgaccgtc tatacggagg
     9181 acggcggcga attcaagctc gccgccgagc tgttttcctt cagcgtcggc cgcctcgcgg
     9241 aatccgacga cttggatttc ctggccggct cggccggtcc cccgggacgg cccttcgaaa
     9301 tgcgcctcac caacgaagcc ctcgagtccc accggatcaa ttggcttcga ccgctggccg
     9361 tcgtccatcc cctggggacc agggtcttcc cggattcgaa caaccggctg gtgctcttcc
     9421 gcaggttcct tccgccgctt gaggcccgaa acagcctggg cgaggacgtc ctccccgcgg
     9481 tcgccgaagc cgatgatcgc gcctaccgca gccctcaatc cct
//
LOCUS       DAOOXH010000094         9279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_8136_length_9279_cov_6.661427, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000094 DAOOXH010000000
VERSION     DAOOXH010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9279
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8136_length_9279_cov_6.661427"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1688)
                     /locus_tag="PKV64_09640"
     CDS             complement(<1..1688)
                     /locus_tag="PKV64_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPN17374.1"
                     /translation="MIQVENLTKRYGGQTLFEGISFKINRRERVGLVGRNGHGKTTLF
                     RLIVGQEEPDEGLISIPRSYRIGYVEQHLGFTAPTVFQEAALSLPRDQRDDFWRVEKI
                     LAGLGFGAADRAKHPAELSGGFQVRLNLAKVLLADHNMLLLDEPNNYLDITSIRWLEK
                     FLNAWPGELILVTHDRSFMDKVVTHTLAIHRRKVKKIEGTTGKLYDQIALEEETYEKT
                     RINDERRRKEIENFIAKFRASARLQGLVESRKKTLAKMGRKEKLERLPSLDFAFTAKP
                     FHGKYVLNIDGLTFGYDPERPLIRDFGLSIGARDRVFIIGPNGKGKTTFLKLLAGRLE
                     PQAGTVVSPMAVTTGYFEQTNVQSLGPQNTVLEEIQNADASIEPAKARFLAGMMMFED
                     DNALKKISILSGGEKSRVLLAKLIAAPVNLLLLDEPTNHLDMDSSDALLAALDEFEGA
                     VIMVTHNELFLDALAERLIVFQDDGLSVFEGTYDRFLEKVGWSTEKDRPRIRPGDAAS
                     GPAAAPPLGRREIRKLRSEIVAEKSKALRPIEQNMGALEKHIEKTEADIARLTR"
     gene            complement(1701..4409)
                     /locus_tag="PKV64_09645"
     CDS             complement(1701..4409)
                     /locus_tag="PKV64_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HPN17375.1"
                     /translation="MTKWWHLNSETTARQLETDLHSGLAEQAVAGRREKYGPNQLREQ
                     KGRTPLRIFIEQFADFMIWVLIGAALVSGFLKEWVDALAIVAIVILNALMGFVQEHRA
                     ERSLQALRKLAAPFSRVIRDGRRVSIPSAELVPGDLVELEAGDNIPADGRIVQHTPNF
                     AVLEASLTGESTPVVKTRLPLEERDIPLADRANMIYMGTSVVSGKGRALIAETGMNTE
                     LGRIAGMIQSITRETTPLQKRLEQFGKFIVYACFILVAVVFGLELLRGGKLLDMFLTS
                     VSLAVAAIPEGLPAVVTIALALGVQRMVKRNALIRKLPAVETLGSASVICSDKTGTLT
                     KNEMTVRAVWTSAGLTQIGGIGYDPSGSFSREAGLPPADGDPDLRACLEAGVYCNGAE
                     LVQRDGRWIILGDPTEGALLTAAAKAGLTRAGLETQCAFVEEIPFDSERKLMSVIRRA
                     ENDGLLTAYVKGAADILLTRCTHLLENGQARLMQKEDRVRIGRIIEQLSNQALRVLAM
                     ARRTFEAPPDAWSDETVERDLTFLGLAAMIDPPREEVKEAIAKCRESGIATVMITGDH
                     KMTAVAIARELGFYGEDSRALTGAELDRLSEAEFEKEVERTAVYARVSPEHKLRVVQA
                     WRRRGQVVAMTGDGVNDAPAVKEADIGVAMGITGTDVTKEVSDMIVTDDNFASIVAAV
                     EEGRGIYDNIKKFIHYLLSCNTGEILTMFVASLIGWPVPLFPIQILWVNLVTDGLPAL
                     ALGMDPVAKDVMARPPRRKDEPIITGRRGRLIIAQGAFIALCALGAFAFVLFVEKEGL
                     GRARTAALFALASSQLFHSLNCRSQRDSLFTLGFFTNMKLIAAIGVSLALQIAILYLP
                     FTQKIFKVEALGPLDLAIVVAVSSFPLWMMELVKRFSRRRREKAEA"
     gene            4645..4719
                     /locus_tag="PKV64_09650"
     tRNA            4645..4719
                     /locus_tag="PKV64_09650"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4680..4682,aa:Pro,seq:cgg)
     gene            4777..6027
                     /locus_tag="PKV64_09655"
     CDS             4777..6027
                     /locus_tag="PKV64_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN17376.1"
                     /translation="MIRDIRGQWRARPEGSRTFLALKHRNYRLWFGGQLTSLFGSWMQ
                     STALGFLVFELTKSPEFLGYVGFAAGIPTWLFMLWAGVVADRIPRRRQMLITQTSEML
                     MAFLASILVFTGAIRPWQIILLAFGFGTANAFDAPARHALVQDLVPLEDMTNAIALNS
                     AMFNTSAAVGPAVGGLIYALVGPGWCFFVNGLSFLAIIAALTRMKIPASAPRPRTSSM
                     SVDLREGLGYVSRQPVIRAIMANLLVVSMFGYAFTTLIPAWAVNILHGNATTSGFLIS
                     ARAIGSLGAALFIASLGRFHFRGKLLALGSFAFPVLMIVFAFLRVHGLAFLFLFGAGF
                     SIILVFNLSNSIVQTLAPDHLRGRIMAIYSLTFFGSMPLGALLIGSLAERLGEPPAVV
                     INSLTALLFCGLMFLAGPRLHAVE"
     gene            complement(6063..7139)
                     /locus_tag="PKV64_09660"
     CDS             complement(6063..7139)
                     /locus_tag="PKV64_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPN17377.1"
                     /translation="MKKSRVYFVEARGAEPAADIDRKSRDVLAPTGFVEKLEPEDFVA
                     LKIHFGEKRNTGYIKPPWIRSLAEEISRQAPRAFLTDSNTLYTGPRSNSVEHIRLAWS
                     HGFTPEILGLPIVIADGLAGGDYGEAAGRGGRVSSAKIAGAILGADALLALSHVTGHV
                     QTGLGAALKNLGMGCASRTGKLDQHAVVHPRVNKKSCRNCGLCLAHCPAGAIRQGTEA
                     VDIDGSRCTGCGECLVACRYGAIRIRWDDDSLRIQEKIAEYALLVARRFPGKTAFLNF
                     LIQVTKDCDCMARNQPPIVPDIGLLASTDPVAVDRASVDLILGRSGGTDVFRKGYDID
                     WSRQLAHAENIGLGMNRYELVRIK"
     gene            complement(7144..8283)
                     /locus_tag="PKV64_09665"
     CDS             complement(7144..8283)
                     /locus_tag="PKV64_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HPN17378.1"
                     /translation="MDPLLLIPGPSPVVPRVTGALARPTVSHASAELAADLLSALDNL
                     KKTVFTAGGEPVLFAGSGTLAMEAALLNVTGKHDRVLVVSQGYFGDRMARIAEAFGFA
                     CDVLAGPWGSAVRPGELRAALDSRRYEVVAVTHVDTGTGAAAPLREYAEVLKDRGSIW
                     IVDGVCATGGIEERMDDWGIDVILTAAQKCFGAPPGLAVDVFSPRAAAKRRSLPSIPA
                     YYTDLLNWRDGMRDPRKYFSTHAVNEIRAFAEAVRIAVEEGLPERFLRHERTARAVRA
                     GLAVLGLEVFTEREFLAPTLSVVRYPDGVDDAAFRAKYLDAGYAVAGGLAETAGRVFR
                     MGHMGNLTRDQALDAIGTMGEVLKSMGRPVDPAEAVAAAAAAFKE"
     gene            8522..8764
                     /locus_tag="PKV64_09670"
     CDS             8522..8764
                     /locus_tag="PKV64_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17379.1"
                     /translation="MGKILAVLGGLVAMAGGVVLVALVWWPQFTCLVKGVIPPLLFFG
                     GLIALIAGVSSFKDAARAKKLESELKEDEKKASPNA"
     gene            8792..9235
                     /locus_tag="PKV64_09675"
     CDS             8792..9235
                     /locus_tag="PKV64_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17380.1"
                     /translation="MTHLGLVLVSFVLSLVSSATTEIEGAFRRNDPERLRPLLEKGSF
                     LNISFSSPDVFSDLLSDEQTLLWLRGFFRSSRTLGFYPEGPAVQSLENGSFVFKARWE
                     VRTRDGRQTAYDILFLIRSASFHPPGSRAPGRGFWKILQIRAERR"
BASE COUNT         1504 a         3020 c         2985 g         1770 t
ORIGIN      
        1 tcccgggtca ggcgggcgat gtcggcttcc gtcttttcga tgtgtttttc cagggcgccc
       61 atgttctgct cgatcggccg gagggctttc gacttttcgg ccacgatctc ggagcggagc
      121 ttccggatct cccgccgtcc cagcgggggg gcggcggccg ggcccgaagc ggcgtccccc
      181 gggcggatcc ggggccggtc tttttcggtc gaccagccga ccttttccaa gaagcggtcg
      241 tacgtccctt cgaagaccga gaggccgtcg tcctgaaaga cgatcaggcg ctcggccaag
      301 gcatcgagaa acagctcgtt gtgggtgacc atgatcaccg ctccctcgaa ctcatccagg
      361 gcggccagca gagcgtcgga ggaatccatg tcgagatggt tggtgggctc gtcgaggagc
      421 aggagattga ccggggcggc gatgagcttg gccaggagaa cgcggctttt ttcgccgccg
      481 gacaggatgc tgatcttttt cagggcgttg tcgtcctcga acatcatcat cccggccagg
      541 aagcgggcct tggccggctc gatcgaggcg tcggcattct ggatctcctc cagcacggtg
      601 ttctggggac ccagggattg gacgttggtc tgctcaaaat agccggtcgt gacggccatc
      661 gggctgacca ccgttccggc ttggggctcc aggcgaccgg ccaggagctt gagaaacgtg
      721 gtcttgccct tgccgttcgg accgatgatg aacacccggt cgcgggctcc gatgctcaag
      781 ccgaaatcgc ggatcagggg ccgctccggg tcataaccga acgtcaggcc gtcgatgttc
      841 aggacgtact tgccgtggaa aggcttggcc gtgaaggcga aatcgagcga cggcagccgc
      901 tccagctttt cctttcggcc catcttggcc agcgtttttt tccgggactc gaccaggccc
      961 tggaggcggg cgctggcccg gaacttggcg atgaaattct cgatctcctt ccgccggcgc
     1021 tcgtcgttga tccgcgtttt ttcgtacgtc tcctcctcca aggcgatctg atcgtacagc
     1081 ttgcccgtgg tcccctcgat ttttttcact ttccggcggt ggatggccaa ggtgtgagtg
     1141 acgaccttgt ccatgaagct gcggtcgtgg gtgaccagga tcagctcgcc cggccaggcg
     1201 ttcaagaatt tctccagcca gcggatcgag gtgatgtcga ggtagttgtt cggctcgtcc
     1261 aggagaagca tgttgtgatc ggccaagagg actttggcca ggttcaagcg gacctggaat
     1321 ccacccgaaa gctcggccgg gtgcttcgcc cggtcggccg ccccgaagcc cagcccggcc
     1381 aggatctttt cgacccgcca gaaatcgtcc cgctgatccc ggggcaggct caaggcggct
     1441 tcctggaaga cggtcggggc ggtgaagccg agatgttgct cgacgtagcc gatccggtag
     1501 ctgcggggga tggagatcaa tccttcatcc ggctcttcct ggccgacgat caagcggaaa
     1561 agcgtcgtct tgccgtggcc gttgcggccg acgagaccga cccgctcccg gcggttgatc
     1621 ttgaaagaga tgccctcgaa cagcgtctgc ccgccgtacc gcttggtgag attttcgact
     1681 tggatcatcg gccgttccgg tcaggcttcg gccttctccc ggcggcgccg gctgaatcgc
     1741 ttgaccagct ccatcatcca cagcgggaac gaggagacgg cgacgacgat cgccagatcg
     1801 agcggcccga gggcttcgac cttgaagatt ttctgggtga acgggaggta caggatcgcg
     1861 atctggaggg ccagcgacac gccgatggcg gcgatcagct tcatgttggt gaagaaaccg
     1921 agcgtgaaca gggaatcgcg ctggctgcgg caattcaggc tgtggaagag ctggctcgaa
     1981 gccagggcga acagggccgc ggtccgggcc cggccgaggc cttccttctc gacgaagagg
     2041 acgaaggcga acgcgcccag ggcgcacagg gcgatgaacg ccccctgggc gatgatcagc
     2101 cggccgcggc ggccggtgat gatcggctcg tccttccggc gcgggggccg ggccatgacg
     2161 tccttggcca ccgggtccat gcccagggcc agggcaggca acccgtcggt caccaggttg
     2221 acccagagga tctggatcgg gaacagcggc accggccagc cgatcagcga ggcgacgaac
     2281 atcgtcagaa tctcgccggt gttgcaggac aggagataat ggatgaattt cttgatgttg
     2341 tcgtagatcc cccggccttc ctccacggcg gcgacgatcg aggcgaagtt gtcgtcggtg
     2401 acgatcatgt ccgagacttc cttcgtcacg tccgttccgg tgatccccat ggccacgccg
     2461 atgtcggctt ccttgacggc cggggcgtcg ttgaccccgt ctccggtcat ggccaccact
     2521 tggccgcggc ggcgccaggc ttggacgacg cggagcttgt gttcgggaga gacgcgggcg
     2581 tagaccgcgg tccgttcgac ttccttctcg aattccgcct cgctcaggcg gtcgagttcc
     2641 gccccggtca gggcccggct gtcctcgccg tagaatccga gctcgcgggc gatggccacg
     2701 gcggtcatct tgtgatcgcc ggtgatcatg accgtggcga tccccgattc ccggcacttg
     2761 gcgatggctt ccttcacttc ctcccgggga ggatcgatca tggccgcaag cccgagaaac
     2821 gtcagatccc gctcgacggt ctcgtccgac caggcgtcgg gcggcgcctc gaacgtccgg
     2881 cgggccatgg ccagcacgcg cagggcctgg ttggagagct gctcgatgat gcggccgatg
     2941 cggacccggt cttctttttg catcagccgg gcttggccgt tctccaggag atgggtgcag
     3001 cgggtgagaa gaatatcggc cgcgcccttg acgtaggcgg tcaggagtcc gtcgttttcc
     3061 gcccggcgga tgaccgacat cagcttccgc tcggaatcga aggggatctc ctcgacgaaa
     3121 gcgcactgcg tttcgaggcc ggccctggtc aagccggcct tggccgcggc ggtcaaaagg
     3181 gccccctcgg tcggatctcc gaggatgatc cagcggccgt ccctttggac gagctcggcc
     3241 ccgttgcaat agacgccggc ttcaaggcaa gcccgcaaat cgggatcccc gtccgccggc
     3301 ggaaggcccg cctcccggct gaagcttccg gagggatcgt acccgatgcc tccgatttgg
     3361 gtcaggccgg ccgacgtcca gaccgcccgg accgtcattt cgttcttggt cagggttccg
     3421 gtcttgtccg aacagatgac cgaggccgag cccagcgtct ccacggccgg gagcttgcgg
     3481 atcaaggcgt ttcgcttgac catgcgctgg acgcccaggg ccaaggcgat cgtgaccacg
     3541 gccggcagtc cctcggggat ggcggccacg gccaggctga ccgaggtgag gaacatgtcg
     3601 aggagcttgc cgccgcggag aagctccagc ccgaagacga cggccacgag gatgaagcag
     3661 gcgtaaacga tgaacttgcc gaattgttca agccgcttct gcaggggcgt ggtttcccgg
     3721 gtgatcgact ggatcattcc ggcgatgcgg cccagttcgg tgttcatccc ggtttcggcg
     3781 atcagggccc ggccttttcc cgaaacgacg gacgtgccca tgtaaatcat gttggcccgg
     3841 tcggccaggg ggatatcgcg ctcttccagg ggaagccggg tcttgacgac cggcgtcgat
     3901 tccccggtca ggctggcttc caagacggcg aaattcggcg tgtgctggac gatgcggccg
     3961 tcggccggaa tgttgtcgcc ggcttccagc tcgacgaggt ctccggggac gagctcggcc
     4021 gacgggatgg agacccgccg gccgtcccgg atcacccggg aaaagggggc ggccagcttg
     4081 cggagggctt gaagagaacg ctccgcccgg tgttcctgga caaagcccat aagggcgttg
     4141 agaatgacga tggccacgat cgccagggcg tcgacccatt ccttgaggaa tcccgagacg
     4201 agggccgccc cgatcaggac ccagatcatg aaatcggcga actgctcgat aaaaatgcgg
     4261 aggggcgtgc ggcccttttg ctcccgaagc tggttgggtc cgtatttttc ccggcggccg
     4321 gccacggcct gttccgccaa gcccgaatga agatccgttt ccagctgccg ggccgtcgtt
     4381 tccgaattca ggtgccacca tttggtcatg tgcgtctctt tgtcgatgaa atatccgcgt
     4441 ctttcggtcg gaagaaggcg tattatacag gagagaaagc gcatttttcc attttgattc
     4501 tcttcggccc gggagggccg tcgccggagc agaacgtcct ccgcccgcgc gcccggatct
     4561 ttgcggcgtt tcccggccgc tcgtgacggg gaagcgaaag cagttgatct ttttcgggat
     4621 ccggctataa taagcgttcc tcgtcgggga gtagcgcagc ctggctagcg cacagcgttc
     4681 gggacgctgg ggtcgtgggt tcgaatccca cctccccgat cacagccctt aatcccatct
     4741 tcaacggatt ccgggtcgag gcggagcatt cggagaatga ttcgcgacat ccggggacag
     4801 tggcgcgccc ggcccgaagg aagccggacc tttctcgccc tgaaacaccg caattaccgg
     4861 ttgtggttcg gaggccagct gacctcgctg ttcggcagct ggatgcaatc cacggccctg
     4921 ggcttcctgg tcttcgagct gacgaaatcg cccgagttcc tgggatatgt cggcttcgcc
     4981 gccggcattc cgacctggct gttcatgttg tgggccggcg tcgtcgccga ccgcatcccc
     5041 cgccgccggc agatgctcat cacccagacg tcggaaatgc tcatggcctt cctggcctcg
     5101 atcctggtat tcaccggggc gatccgcccc tggcaaatca tcctcctggc cttcggcttc
     5161 ggcacggcca acgccttcga cgccccggcc cgccacgccc tggtccagga cctggtcccc
     5221 ttggaggaca tgaccaacgc catcgccctg aactcggcca tgttcaacac ctcggccgcc
     5281 gtcggcccgg ccgtcggagg gctgatctac gccttggtcg ggccggggtg gtgctttttc
     5341 gtcaacggcc tgtctttcct ggccatcatc gccgccctga caaggatgaa gatcccggcg
     5401 tccgcccccc gcccccgaac atcctcgatg agcgtggatc ttcgggaagg gctcgggtac
     5461 gtttcccgcc agcccgtcat ccgggcgatc atggccaacc ttctcgtggt cagcatgttc
     5521 ggttacgctt tcacgacgct catccccgcc tgggcggtca acatcctcca cgggaacgcg
     5581 acgacgagcg gattcctcat ctccgcccgg gctatcggct cgctcggcgc ggccctgttc
     5641 atcgcctcgc tcggccgatt ccatttccgg ggaaagctcc tggccctcgg atccttcgcc
     5701 ttccccgttc tgatgatcgt cttcgccttc ctccgcgttc acgggctcgc ctttctcttc
     5761 ctgttcgggg cgggattctc catcattctc gtcttcaacc tgtcgaactc cattgtccaa
     5821 accctcgccc ccgaccacct tcgcggacgg atcatggcga tttattccct gacgtttttc
     5881 ggctcgatgc cccttggggc gttgctgatc gggtccctgg ccgagcgtct cggcgagccg
     5941 ccggccgtcg tcatcaattc cctgacggcc ctgctctttt gcggcctgat gttcctggcc
     6001 ggccctcgtc tccacgcggt cgagtgaccg gcgggccgaa ccattccctc cgcccgggcc
     6061 ctctatttga tccggacgag ctcgtagcgg ttcatcccca ggccgatgtt ctcggcatgg
     6121 gccagctggc gggaccagtc gatatcgtac cctttgcgga agacatccgt tcctccggac
     6181 cggcccagga tgagatcgac cgacgcccga tcgacggcga ccgggtcggt cgaagcgagg
     6241 aggccgatgt cggggacgat cgggggttga ttgcgggcca tgcagtcgca gtctttggtc
     6301 acctggatca agaagttgag aaaggcggtt ttccccggga atcttcgggc gacgaggagg
     6361 gcatactcgg cgatcttctc ctggatccgg agggaatcgt cgtcccagcg gatccggatc
     6421 gctccgtacc ggcaggcgac caggcattcg ccgcatccgg tgcagcggga tccgtcgatg
     6481 tccacggcct ccgtcccctg ccggatcgcc ccggccggac aatgggccag gcagagcccg
     6541 cagttccggc agctcttctt gttcacccgc ggatggacca cggcatgctg gtcgagcttc
     6601 cccgtccggg aggcgcagcc catccccagg ttcttgaggg ccgcgccgag gccggtctgg
     6661 acatgcccgg tgacgtggct caaggccagc aaggcgtccg cgccgaggat ggccccggcg
     6721 atcttcgccg aggaaacgcg cccgccccgg cccgcggctt ccccgtagtc gccgccggcc
     6781 aggccgtcgg cgatgacgat cggaaggccg agaatttcgg gagtgaatcc gtggctccag
     6841 gccagccgga tatgctccac ggaattcgac cggggaccgg tgtagagcgt gttggaatcg
     6901 gtcaggaagg cccggggcgc ctgccgggag atctcctccg ccagcgaccg gatccagggc
     6961 ggcttgatat atcccgtgtt cctcttctcc ccgaaatgga ttttgagggc gacgaagtcc
     7021 tccggctcca gcttctccac gaatccggtc ggggcgagaa cgtcccgcga tttccggtcg
     7081 atgtcggcgg ccggttcggc gcctcgggcc tcgacgaaat agacccggct tttcttcatc
     7141 ctctcattcc ttgaacgccg ccgcggccgc ggcgacggct tcggccgggt cgaccggccg
     7201 gcccatggat ttcagaacct cgcccatcgt tccgatcgcg tccaaggcct ggtcccgcgt
     7261 caggtttccc atgtgcccca tccggaacac ccggccggcc gtttcggcca gcccaccggc
     7321 cacggcgtag ccggcgtcca ggtacttcgc ccggaacgcg gcgtcatcga cgccgtcggg
     7381 atagcggacg accgacaggg tgggagccag gaattcccgt tccgtgaaca cctcgagccc
     7441 caggaccgcc aagcccgccc ggacggcccg ggccgtcctc tcgtggcgga gaaaccgctc
     7501 cggcagcccc tcctcgacgg cgatccggac cgcctcggcg aacgcccgga tttcgttgac
     7561 ggcgtgggtc gagaaatatt tccggggatc cctcatgccg tcccgccagt tcaaaagatc
     7621 ggtgtaatac gccgggatcg aaggcaggct ccgccgcttg gccgccgccc ggggcgaaaa
     7681 gacgtcaacg gccaggccgg gcggggcgcc gaaacacttt tgggcggcgg tgagaatgac
     7741 atcgatgccc caatcgtcca tccgctcctc gatcccgccc gtcgcgcaga cgccgtcgac
     7801 gatccaaatc gagccgcggt ccttcaggac ctcggcgtac tcccggagcg gggcggcggc
     7861 ccccgtgccc gtgtccacgt gagtgacggc caccacctcg tagcggcgcg aatcgagggc
     7921 cgcccggagc tcgccgggcc ggaccgcgct cccccacggg ccggccagga cgtcgcaggc
     7981 aaacccgaag gcctcggcga tgcgggccat ccggtctccg aaatacccct gcgacacgac
     8041 aaggacgcgg tcgtgtttcc cggtcacgtt cagaagggcc gcttccatgg ccagcgtccc
     8101 cgacccggcg aaaaggaccg gctccccccc ggccgtgaag accgttttct tgagattgtc
     8161 gagggctgaa agcaaatccg cggccagctc ggcgctcgcg tggctgacgg tcggacgggc
     8221 cagggctccg gtgacgcgcg gaacgaccgg gctgggcccc gggattaaaa gaagcggatc
     8281 catgaacgac tctccttgac cggcgtttcc ccgctattgt catcgaagga cggcaaggaa
     8341 tcaagaagcg gcttgctccc ggccggacgg ccgcaaaccg ccggactggg tcggatccat
     8401 tcgggacgcc tttggggcaa gattggagtc gccatgtttc ccccatccaa tcgcttgcgt
     8461 ctccgaatcg cgttctgtat aatgcggaac gaagagatga tcgaatttag ggaggccgtt
     8521 catgggtaaa atcctcgcgg ttttaggcgg tttggtcgcg atggccggag gcgtcgttct
     8581 cgtcgctctc gtctggtggc cccaattcac ctgcctcgtc aagggagtca tccctcccct
     8641 tctgttcttc ggcgggctga tcgccctgat cgccggcgtg agcagcttca aagacgccgc
     8701 ccgggccaag aagctcgaat ccgaattgaa ggaagacgag aaaaaagcct ccccgaacgc
     8761 ctgaccggcg gtcgtccggt ctcagccccg cgtgacgcat ctcgggctgg tccttgtctc
     8821 cttcgtcctg tccctggttt cctcggccac gacggaaatc gaaggagcat tccgccggaa
     8881 tgatcccgag cggctgagac ccttattgga aaaagggtcg tttctaaaca tctcgttttc
     8941 cagcccggac gttttttccg acctcctttc ggacgaacag accctccttt ggttgcgcgg
     9001 atttttccgg tcctcgagga ccttgggatt ctatccggaa gggccggccg ttcaatcgct
     9061 cgaaaacggc tccttcgttt tcaaggcccg gtgggaagtc cggaccaggg acggccggca
     9121 gaccgcctat gacatcctgt ttctgatccg gagcgcgtcg tttcatcctc ccggaagccg
     9181 cgctccgggc cggggatttt ggaagatcct ccagatccgg gcggagcggc ggtgagaaga
     9241 aaagcgtttc ggccggcctg ggccgccgcg atcctggcc
//
LOCUS       DAOOXH010000095         8797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_8795_length_8797_cov_7.741707, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000095 DAOOXH010000000
VERSION     DAOOXH010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8795_length_8797_cov_7.741707"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PKV64_09680"
     CDS             <1..405
                     /locus_tag="PKV64_09680"
                     /inference="COORDINATES: protein motif:HMM:NF028226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HPN17381.1"
                     /translation="KEFLVLAAGDSGRVEAVRLLREAFMKLLDASIEEAGQMDIMKAL
                     ARHYGVFCDFANIRTRTAGRRQFVDLTLILPAGLSIKKAHGHAADLKNDLAASIPESE
                     IVVHMEPCAGDCAYAAEGRACPYLVPAGKNGA"
     gene            621..1358
                     /locus_tag="PKV64_09685"
     CDS             621..1358
                     /locus_tag="PKV64_09685"
                     /inference="COORDINATES: protein motif:HMM:NF028425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HPN17382.1"
                     /translation="MRAARFPGILILVFAFSLIGAACRERRPEPKLETHRTIPSQGES
                     RAVVTIKMAAGELDVRGGAGGELMKGSFYYNRRPWEPRIDYQVSGGVSRLVVEQRRMG
                     PAFGRVRNNWNVELSRKIPTELRLDLGVGKARIDLDGVPVTKLDVQVGVGGMDLDLSG
                     ARNEGLTGQIEGGIGRAEILLPSDIGVRLRIEGGLGSIHAPGFIKDGETFRNEAWGKT
                     GASLDLAIEAGIGRIDLRMKNTRSFSF"
     gene            1551..3809
                     /locus_tag="PKV64_09690"
     CDS             1551..3809
                     /locus_tag="PKV64_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPN17383.1"
                     /translation="MRKLALILSFFAASTAVAFAQDVFDLLRKGDVSAVKALIERTPE
                     TVNRRDDNGWTPLHYAASAGDAALVAYLIEKGATVEAEDASRKTPLHRAAMNDRREVV
                     AELLRRGAALEARDSYERTALVLCARERGGAATARILLKAGADIEAVDKFGDDALGLA
                     AWRGKADFVDLLLEKGAPVPDQGRKWGQGISLAASQGLTNLFRRLAGPGKDLKAVDPS
                     GTELLHMAAAGGSDVIIALLLENGFDPARPDRFGWTPLHYAARDGRTGAARILIERKI
                     PLDARTAAGQTAYDVARERGASAVAGLLAEAGADTSGVRFPVLEGDYLGQKPPAGKAE
                     LFGLGVISSIWGLHSTAVFSPDGNEVYWAPMVTYPGEAYSRGGLLMMKRVDGRWSAPA
                     PAAFSRPGDDDDVPFFSPEGQTIYFLSRRLLPGETAAPGERIWLADRTPGGGWSEPRF
                     LDPAVNAHNMHWEFSLDKSGNVYFAGEGADSSGMTDIYFARRTGGKYEAPVNLGGSIN
                     SPGPETTPFVAPDGSYLIFSRDYDLWASFREDGGAWSAPVNLGPDVNSPSIELCPIVT
                     ADSRYLFFLSQRDGESHVYWVRAGVIEEARPKPAEQTPGGGARAEIERSIRAAVGWAQ
                     TKDFGLLYRVIAGDADFLEVHPDGRVVKGIEEFKRAEAVWGSPDFKAVRCDIRDLKIT
                     ISKSGDTAWFFGILDDINTWKGKPANWENTRWTGVLEKRDGRWVIVQQHFSFAEKDGP
                     GAAGPAAIRPAP"
     gene            complement(3806..7093)
                     /locus_tag="PKV64_09695"
     CDS             complement(3806..7093)
                     /locus_tag="PKV64_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPN17384.1"
                     /translation="MKSLVACFVSAILFLAVSAQAAPESRLLREPDISATRIVFAYGG
                     DLWTVPRTGGDAVRLTSFPGVESNPSFSPDGNLVAFSAAYDGNTDVYVVPSAGGEPRR
                     LTWHPGEDLVRGWTPDGGRVLFASGRTGAPLSYPKLWTVALGGGMPEPLPLPRVFKGA
                     FSPDGRRMAYQMIEPWESEFRNYRGGQNNPLRLIDLETLEEEKLPWDGANDNTPVWLG
                     DTVYFLSDRDYAMNVWACDLAAREVRQITHFTDFDAKQIAGDGGTLVIEQGGGLHTLD
                     LADGTLEKIAVSVRGDFAWARPHWIKAGERLTEPALSPTGKRAVFSARGEILSVPAEK
                     GDPRNLSGDPAAADRSPAWSPDGEKIAWFSDASGEYQLVIADQKGENRSVIPLPKPTF
                     FYSPAWSPDSSSIAFTDANRDLWIARVASKAITRVDTEGFAHPERIIFPCWSPDSKWL
                     AYAKRLRNEYGAIFVYSVDKGTATAVTDGMSDCRMPVWDASGKYLYFLASTDYGLNVG
                     WLDLSSFERPITRSLYAVVLAKDGPDPLALESDEEAAAKAEDKGPAGKKPGKNKAEAA
                     GDADKGKDKAVPAVNIDFDGLARRIVPLPVKARDYRLLAGGEAGTLFLGELIPGEESL
                     LVHRWDAKERKTKEYLSGVSDLAVSADGKKVLYGLDGKTWGIVDASGSPKPGDGKIAA
                     GDLRLYVDPAAEGRQIFKEAWRFQRDYFYVRNVHGADLDGLYRVYAPWVEHVRHRADL
                     NYLLDILGGETSIGHSFVGGGDMPAVESVPVGLLGADFTLDSGRYKIARIYTGESWNP
                     NLKAPLAGPGIDVKEGDYLLAVNGREIEIGRSLFSYFERTAGVQTRIKVNAKPEPAGA
                     REITVVPVRSEEALRRAGWIEDNRRRVDALSGGKLAYVWLPDTADGAYINFNRYFFAQ
                     KDKKGAVIDERWNQGGQIADYVIDLLARPLLGYFNNPIGDKQPWTAPNAAIFGPKVMI
                     INDAAGSGGDMMPYMFKERGIGPLVGTRTWGGLVGIWDVPALIDGGRITAPRGGFYNT
                     AGEWDVENKGVAPDIEVEMSAKSIAAGGDPQLEKAVEAAMEALKTGEVKLLPQPPDPV
                     RVLRPRR"
     gene            complement(7527..>8797)
                     /locus_tag="PKV64_09700"
     CDS             complement(7527..>8797)
                     /locus_tag="PKV64_09700"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HPN17385.1"
                     /translation="TAIGRAKDIKLKLPFIVPGMGSTNVAKNNWAGIAIGAAISGAVL
                     TVGENVCGMDEESEIKNGRVVRSPDMEMRVRSFQDWSDGYGDVVVQANIEDGRLGVQE
                     YAIEKLGVETVELKWGQGAKDIGGEVKIKNLKKAQELKRRGYIVLPDPEDPDIIASFE
                     KGSFREFERHSRMGMVEEETFMKRVEDLRRAGAKRVFLKTGAYRPADLARAVKYASKA
                     KLDLLTVDGAGGGTGMSPWRMMNEWGVPSVELWSLTHQFAKKLDKKGEYVPDICFAGG
                     VTFEDQIFKALALGAPYVKAVGMARSVVAAAMVGKTIGKRVDEGQIPVYVERFGSSVD
                     EIFVTAPKLKRRFGKRYADLPPGAMGVYSYFARLSQGLRQLMAGNRKFGLSYITRDDI
                     AALTKEASDISGIPYVLDTDKEAVEKILKG"
BASE COUNT         1447 a         2819 c         2898 g         1633 t
ORIGIN      
        1 aaagagttcc tcgtcctggc cgccggcgac tccggccggg tcgaggccgt ccggcttctg
       61 cgggaggcgt tcatgaagct gttggacgcg tccatcgagg aagccgggca gatggatatc
      121 atgaaagccc tggcccgtca ttacggcgtt ttctgcgatt tcgcgaatat ccggacgagg
      181 acggcgggcc ggaggcagtt cgtcgacctg accctgatcc tgccggccgg tctttcgatt
      241 aaaaaagccc atggccatgc cgcggatctg aaaaacgact tggccgcctc cattcccgag
      301 agcgaaatcg tcgtccacat ggagccatgc gccggggact gtgcctacgc ggccgagggc
      361 cgggcctgtc cttatctcgt cccggcggga aaaaacggcg cgtgagcctt ctcttcttat
      421 catgaaaaca tggacggccg gaaagaaaac ccggatcacc cgcgctttag ccggccgaag
      481 caacgtcttc ctggtctccg gcggagggaa gagccttctg gtcgatatca gcccaaggtc
      541 cctccgccgc cggtcccgcc gtgaacccct cgcactccct ttccgtatat aatagcgaca
      601 tcaagcagag gaggtttccc atgcgcgccg cccgttttcc cgggattttg atccttgttt
      661 tcgcctttag cctgattggc gccgcctgcc gggagagacg cccggagccg aagctggaaa
      721 cccaccggac gatcccctcc caaggcgaaa gccgggccgt cgtcacgatc aaaatggccg
      781 ccggtgagct ggacgtccgc gggggcgcgg gcggcgagtt gatgaaagga tctttttact
      841 acaaccggcg gccgtgggaa ccccggatcg attaccaagt ctccggcggc gtctcccgcc
      901 ttgtggtcga acagcgccgg atgggcccgg cgttcggccg cgtccggaac aattggaacg
      961 tggagctgtc ccggaagatc ccgacggagc tgcggctgga cctcggcgtc ggaaaagcca
     1021 gaatcgacct ggacggcgtc cccgtcacca agctcgatgt ccaggtgggc gtgggcggca
     1081 tggacctcga tctttccggg gcgcggaacg agggcctgac cggacagatc gagggcggaa
     1141 tcggccgggc tgaaatcctc ctgccgtccg atatcggcgt ccggctccgg atcgaaggcg
     1201 ggctcggctc gatccatgcg ccgggcttca tcaaggacgg cgagacgttc cgaaacgagg
     1261 cctgggggaa aaccggggcc tctctggacc tcgcgatcga agccggcatc ggccgcatcg
     1321 acctgcggat gaagaacacg cggtcctttt cgttctgagc ccgcggtttt tcccggttga
     1381 caagccgtca tggcggcctt tcagccgaag tcggctcctt ttgatccagg ctgtaaagta
     1441 aatttcgaga cgtggtaaaa cggctttacc agggacggcg ggcgtctcga gaggagattt
     1501 ggcctgaaga ttgcaaaacg aaggacaagg tcgttttgcg aggagggccc atgagaaagc
     1561 tcgctctgat tctttcgttt ttcgcggcct cgaccgccgt cgccttcgcc caggacgtct
     1621 tcgacctgct tcgcaaaggg gacgtttcag ccgtcaaggc gttgatcgaa agaacgcccg
     1681 agacggtcaa tcgccgggac gacaacggct ggacccctct tcattatgcc gcttcggccg
     1741 gagatgccgc ccttgtcgcc tatctcatcg aaaagggcgc gacggttgag gccgaggacg
     1801 cgtcccgaaa aacgcccctt catcgggcgg ccatgaacga tcggcgggag gtcgtggcgg
     1861 aacttctccg gcggggcgcg gccctcgaag cccgggacag ctacgagagg acggcgctcg
     1921 tcctctgcgc ccgggaacgc ggcggggcgg cgacggcgcg gattctcctc aaggccgggg
     1981 cggacatcga ggccgtggac aagttcggcg atgacgccct gggattggcg gcctggaggg
     2041 gcaaagccga tttcgtcgac cttcttctcg aaaagggcgc cccggttccg gaccaggggc
     2101 ggaaatgggg gcaggggatc tccttggcgg cttcccaggg gttgacgaat ttattccgcc
     2161 gcctcgccgg tcccggaaaa gatctgaagg ccgtcgatcc ttccgggacg gagctccttc
     2221 atatggccgc ggccggtggt tcggatgtca tcatcgccct ccttctcgaa aacggattcg
     2281 atcccgcccg ccccgaccgc ttcggctgga cgccgcttca ttacgccgcc cgggacggcc
     2341 ggaccggcgc cgcccggatt ctcatcgaaa gaaagattcc cctggatgcc cggacggccg
     2401 ccggccaaac ggcctacgac gtcgcccggg agaggggggc ttcggccgtg gccggccttt
     2461 tggccgaggc cggggctgac acgtccggcg tccggttccc tgtcctggaa ggagattacc
     2521 tcgggcaaaa acctcccgcc ggcaaggcgg agctgttcgg tctgggtgtc atctcctcga
     2581 tctggggcct ccacagcacg gccgtgtttt ccccggacgg aaacgaggtt tattgggcgc
     2641 cgatggtgac ttatccgggc gaggcgtatt cccggggcgg cctgctcatg atgaagcgcg
     2701 tcgacgggcg ctggtccgct ccggctcccg ctgctttctc ccgtcccggc gatgacgacg
     2761 acgttccgtt tttctcacct gagggacaaa cgatctattt cctctcccgg cgccttctcc
     2821 ccggggaaac ggcggccccg ggggagagga tttggctggc cgacaggacg ccgggcggcg
     2881 gttggtccga gccccggttt ctcgacccgg ccgtcaacgc tcacaatatg cattgggaat
     2941 tctccttgga caaatccggg aatgtttact tcgcggggga gggagccgat tcctcgggga
     3001 tgacggacat ttatttcgcc cgtcggaccg gcggaaaata cgaggcgccg gtgaacctgg
     3061 gcggctcgat caactcgccc ggaccggaaa ccacgccttt tgtcgccccg gacgggagct
     3121 atctgatctt ttcccgcgat tacgaccttt gggcgagctt ccgggaggac ggtggcgcct
     3181 ggtccgcgcc ggtcaatctc gggccggacg tcaacagtcc ttccatcgag ctctgtccca
     3241 ttgtcaccgc cgacagccgg tatcttttct tcttgagcca gcgggacggc gaaagccacg
     3301 tctactgggt tcgggccggc gtcatcgagg aggcccggcc gaaaccggcc gaacaaacgc
     3361 cgggcggcgg cgcgcgggcg gaaatcgagc gctccatccg ggcggccgtc ggctgggccc
     3421 aaaccaagga tttcggcctg ctctaccgcg tcattgccgg cgatgctgat ttcctcgagg
     3481 tgcatccgga cggccgggtc gtcaagggaa tcgaggaatt caagagagcc gaggccgtct
     3541 ggggcagccc cgatttcaag gccgtccgct gcgacatccg cgatctaaag atcacgatct
     3601 cgaagtccgg cgatacggcc tggtttttcg gcatcctcga cgacatcaac acctggaagg
     3661 ggaagccggc caactgggag aacacccgct ggaccggggt cctggagaaa cgggacggcc
     3721 gctgggtgat cgtccagcag cacttctcct ttgctgaaaa ggacggtccc ggcgccgccg
     3781 ggcccgcggc gatccggccg gcgccttagc ggcgcggccg gagaacgcgg accggatcgg
     3841 gaggctgggg caggagcttg acctcgccgg tcttgagcgc ttccatggcc gcctccacgg
     3901 ctttttccaa ttggggatct ccgccggcgg ctatgctttt cgcgctcatt tcgacttcga
     3961 tgtcgggggc gacgcccttg ttctccacgt cccattcccc ggccgtgttg tagaagcctc
     4021 cgcggggggc ggtgatccgc ccgccatcga taagagccgg cacatcccag attccgacga
     4081 gcccgcccca cgtccgggtg cccaccagcg ggccgatgcc ccgctccttg aacatgtagg
     4141 gcatcatgtc tccgccggaa ccggccgcgt cgttgatgat catcaccttg gggccgaaga
     4201 tcgcggcgtt gggcgccgtc cagggctgct tgtcgccgat cgggttgttg aaatagccga
     4261 gaagcggccg ggccaggagg tcgatgacgt aatcggcgat ttggccgccc tggttccagc
     4321 gctcgtcgat caccgcgccc ttcttatcct tttgggcgaa aaagtaacgg ttgaagttga
     4381 tgtaagcgcc atcggccgtg tccggcagcc agacataggc cagcttgcct ccggacaagg
     4441 cgtcgacccg gcggcggttg tcctcgatcc agccggcccg gcggagggcc tcctcgctgc
     4501 ggaccggaac caccgtgatt tcccgggccc cggccggctc cggcttggcg ttgactttga
     4561 tccgggtttg gacgccggcc gtccgctcga agtagctgaa gaggctccgg ccgatctcga
     4621 tctcgcggcc gttgacggcg agcaggtagt cgccttcctt gacgtcgatt cccggtccgg
     4681 ccagcggggc cttcaggttg ggattccagc tttcccccgt atagatccgg gcgatcttgt
     4741 agcgtccgga gtcgagggtg aagtcggcgc ccagcaggcc gacggggacg gattccacgg
     4801 cgggcatgtc cccgccgccg acaaacgaat gcccgatgga ggtctcgccg ccgaggatgt
     4861 cgagaagata gttgagatcg gcccggtgcc ggacgtgctc gacccagggg gcgtagaccc
     4921 ggtaaaggcc gtcgagatcg gccccgtgga cgttgcggac gtagaaataa tcccgttgga
     4981 agcgccaggc ttccttgaag atctgccggc cctcggccgc cggatcgaca tagaggcgga
     5041 ggtcgccggc ggcgatcttg ccgtcgcccg gcttgggcga tccggacgcg tcgacgattc
     5101 cccaagtctt gccgtccagg ccgtacaaga ctttcttgcc gtcggccgag acggcgaggt
     5161 cggacacgcc gctcaggtat tccttggttt tccgctcctt ggcgtcccag cggtggacga
     5221 gaaggctttc ctctccggga atcagctcgc cgaggaaaag cgttcccgcc tcgccgcccg
     5281 ccagcagccg gtaatcgcgg gccttgacgg gaagcgggac gatgcgccgg gcgagaccgt
     5341 cgaaatcgat gttcacggcc ggaacggcct tgtccttccc cttatccgcg tccccggcgg
     5401 cttcggcttt attcttgccc ggctttttgc cggccgggcc cttgtcctcc gccttggccg
     5461 cggcttcctc gtcgctttcg agggcgagcg gatcagggcc gtccttggcc aaaacgacgg
     5521 cgtaaaggct ccgggtgatc ggacgctcga aagaagacag gtcgagccag ccgacgttga
     5581 gaccgtagtc ggtggaggcg aggaaataca ggtatttgcc ggaggcgtcc cagaccggca
     5641 tccggcagtc cgacatgccg tcggtcacgg ccgtcgccgt tcccttgtcc acggaataga
     5701 cgaagatcgc cccgtattcg ttgcggagcc gcttggcata ggcgagccat ttcgaatcgg
     5761 gggaccagca ggggaagatg atccgctcgg gatgggcgaa gccctcggtg tcgacgcggg
     5821 tgatcgcctt ggaggcgacc cgggcgatcc agagatcgcg gttggcgtcg gtgaaggcga
     5881 tcgaggagga atccggcgac caggccggag agtagaaaaa ggtcggtttg ggcaggggga
     5941 taaccgacct gttttcgcct ttttgatcgg cgatcaccag ctggtattcg cccgaggcgt
     6001 ccgagaacca ggcgatcttt tccccgtccg gcgaccaggc cgggctgcgg tcggccgcgg
     6061 ccggatctcc gctgagattc cggggatcgc ccttctcggc cggaacgctg aggatttcgc
     6121 cccgggcgct gaagacggcc cgtttccccg tgggggataa agccggctcg gtcagcctct
     6181 ctccggcctt gatccagtgg gggcgggccc aggcgaaatc gccccggacg gacacggcga
     6241 ttttttcgag cgtgccgtcg gcgaggtcca gggtgtggag accgccgccc tgctcgatca
     6301 ccagggtgcc gccgtcgccg gcgatctgct tggcgtcgaa gtccgtgaag tgggtgatct
     6361 gacggacctc ccgggcggcg aggtcgcagg cccagacatt catggcgtag tcgcggtccg
     6421 acaggaaata gaccgtatcg ccgagccaaa ccggtgtgtt gtcgttcgct ccgtcccagg
     6481 gaagcttctc ttcctccaac gtctccaggt cgatgaggcg gaggggattg ttctggccgc
     6541 cgcggtagtt gcggaactcg gactcccacg gctcgatcat ctggtaggcc atccgccggc
     6601 cgtcggggga aaaggcaccc ttaaagaccc gcggaagggg cagcggctcg ggcattcctc
     6661 cccccagggc gaccgtccat agtttcgggt acgagagcgg ggctccggtt cgtcccgagg
     6721 cgaacagcac ccggccgccg tcgggcgtcc agccgcggac gagatcctcg cccggatgcc
     6781 aggtaaggcg gcggggctcg ccgccggcgg acggaaccac gtagacgtcg gtgtttccgt
     6841 cgtaagccgc ggagaaggcc accagatttc cgtcgggcga aaagctcggg ttgctttcca
     6901 ctcccggaaa ggacgtcagc cgcacggcgt cccctcccgt gcggggaacc gtccagagat
     6961 cgccgccgta ggcgaagacg atccgcgtcg ccgagatatc gggctcgcgg agcagccggc
     7021 tttccggcgc ggcctgggcc gagacggcca ggaacaaaat cgcggacaca aaacaggcga
     7081 ctaaggattt catggggtcc tcctcagggg agatggaaat gccgccgcgg cggaacgacc
     7141 gttccccgcc ggcgtattcg ttctcatgga tcgatgattt cgtccttaga ccggaaagcg
     7201 attttatcgc ctggaaccgg ccgagtcaac ggcctgaaaa tccctgtcac gtaaacatta
     7261 aaagtggact cctcccctcg gggccgtaga tgccccggcc ttcgcgtttc tttgcggaaa
     7321 ttctcttttc tcaaggaggc gggattttcc tcgttttccg gaatctcgga ccgctctttc
     7381 cgcccgggat ccggcggatc gactgcttgt cgcggccgcg attcaaggcc gggcggatgt
     7441 cttcgtcccc ggcgatcata agtcgtcggc cggcgccgcc gggttccccg cccggctggc
     7501 gacgccgaga cggttttgaa taacgatcaa cccttgagga tcttttcgac cgcctccttg
     7561 tcggtgtcga ggacataagg gatgccgctg atgtcggagg cttccttggt cagggccgcg
     7621 atgtcgtccc gggtgatgta ggacagcccg aacttgcggt tgccggccat cagctgccgc
     7681 aggccctggc ttagccgggc gaagtagctg tagaccccca tggccccggg cggcaggtcg
     7741 gcgtagcgct tgccgaaacg ccgtttcagc ttgggcgcgg tgacgaagat ttcgtccacg
     7801 gagctcccga accgctcgac gtagaccggg atctggccct cgtcgacgcg cttgccgatg
     7861 gtcttgccga ccatggccgc ggccacgacg ctccgggcca tgcccaccgc cttgacatag
     7921 ggggccccca gggccagggc cttgaagatc tggtcttcga aggtgacccc gccggcgaag
     7981 cagatgtccg ggacgtactc gcccttcttg tccagcttct tagcgaattg gtgggtcagg
     8041 ctccagagct cgaccgaggg gacgccccac tcgttcatca tccgccaggg gctcatgccc
     8101 gttccgccgc cggccccgtc gacggtcagc aggtcgagct tggccttgga ggcgtacttg
     8161 accgcccggg ccaggtcggc cggccggtag gcgccggtct tgaggaagac gcgcttggct
     8221 cccgcccgac gcaagtcctc gacccgcttc atgaacgtct cctcctcgac catgcccatg
     8281 cgggagtgtc gctcgaattc cctgaagctg cccttttcga aggaggcgat gatgtcgggg
     8341 tcctcggggt cgggcaggac gatgtatccg cgtcgcttga gctcctgggc ctttttcaag
     8401 ttcttgatct tgacctcgcc gccgatgtcc ttggccccct ggccccactt aagctcgacc
     8461 gtttccacgc cgagtttctc gatggcgtat tcctggacgc cgagccgtcc atcctcgatg
     8521 ttggcctgga cgacgacgtc gccgtagccg tccgaccagt cctggaagct ccggacccgc
     8581 atctccatgt ccggcgagcg gacgacccgg ccgttcttga tctccgactc ctcgtccatc
     8641 ccgcagacgt tctcgcccac ggtcaagacg gccccgctga tggccgcccc gatggcgatc
     8701 ccggcccagt tgttcttagc cacgttggtc gagcccatgc ccgggacgat gaagggcagc
     8761 ttgagcttga tgtccttggc ccggccgatg gccgttt
//
LOCUS       DAOOXH010000096         8695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_8933_length_8695_cov_7.626968, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000096 DAOOXH010000000
VERSION     DAOOXH010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 8695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8695
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8933_length_8695_cov_7.626968"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1646
                     /locus_tag="PKV64_09705"
     CDS             <1..1646
                     /locus_tag="PKV64_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPN17386.1"
                     /translation="LAEVGEALQALHKAYPGLTTLEEIGRSEEGRPIWAMTVNNPKTG
                     PALAKPGVYVDGNIHGNEIQGGEISLYLLDYLLGRYGKNEEVTALVDKNCFYVVPVVN
                     VDGRYHFLADPNNASSGRGLRIPIDDDRDGLLDEDPPDDLDNDGNITQMRRKDPFGQY
                     KTNPEDPRLTVRIKPGERGEWTLLGEEGIDNDGDGRVNEDAEGYVDPNRQWGFDWAPS
                     YVEGGAGEYPFQGRGLRALAGWMMTKTNICLGWSFHNFGGMFLRGPTRKGLGDYPPQD
                     VAVYDYIGRQAERITPGYRYMVSWKDLYTTYGDSVEWLVRHLGAYGYCGEVFQASAES
                     FKTGEEAERTTPRGEVEQMLRGDFWALERIKYSDNLTMGEMYKPWAPFKHPTYGDIEI
                     GGWVKMSNRLSAPFMLPDLVHRNAMSVLFSAKHTPEVTLDVTEVKKIGDGLFRVRTRL
                     ANAKAVPSMSFQAQKDRLYPQDRLTVSGPNARVMAGGVLVDRDFDRVSHKKDRPDVQF
                     LVVPGFGRVEHQFLVEGRGEVTLRYVSRHAGIREVRISL"
     gene            complement(1630..2079)
                     /locus_tag="PKV64_09710"
     CDS             complement(1630..2079)
                     /locus_tag="PKV64_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPN17387.1"
                     /translation="MESRRPPRRYRNPFPTVDIIIEIRDARGREGIVLIERKNPPPGW
                     ALPGGFVEYGESLETAAVREAWEETSLRVRLVEQLGTYSDPARDPRRHTISTVFIARA
                     EGTPAARDDAKNLGIFTRREIDFPLAFDHAEILKDYYARKRRLKARS"
     gene            complement(2285..3637)
                     /locus_tag="PKV64_09715"
     CDS             complement(2285..3637)
                     /locus_tag="PKV64_09715"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPN17388.1"
                     /translation="MRRKRPRGEPDVFLPPLSGPNPLAPRRPPRKKRPAGRPLRVAAA
                     AALVVLLIAAAVLVFRPFGPGREIVPAVDSAESLQPAEPGPTTVEEVILPGETLSEIL
                     GNEGLSPAQIDALVRQVRPVFNLARIVSGRTVKMTFAPSGELLSLDYPIDESQFLLVT
                     RTGEGYAAEKKAYAYETRPVYVHGVIDEHLYGAVLAGGEKAGLAIELEQIFGWDIDFY
                     AGLRTGDAFRVLVEKKYLDGRFQRYGDILAAEFWNDGRRIEACRFDYEEGGSLKSGYF
                     HPDGRSLRKEFLKSPLQSYRITSRFSSRRLHPVRKVFRAHYGVDYAAPVGTPVFATAD
                     GRVEEAGWKGGAGRMIRLSHKNGYETIYMHLSTILVRAGQRVEGGQRIGKVGSSGEST
                     GPHLDYRIKRFGSYLNPLSAKFMPVAPLPARWLPDFQKRAAALRVVLGAPLRITGSFR
                     "
     gene            complement(3722..5176)
                     /locus_tag="PKV64_09720"
     CDS             complement(3722..5176)
                     /locus_tag="PKV64_09720"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPN17389.1"
                     /translation="MKTLRPIVCAVLMLAVALAAGAQDKPKLDPGVAKALAGIRGSEA
                     YAYVERMASPEFAGRYTGHEGYTAAARWAAGLFKKWGLKPVDPKTGYLLPYPSPYTVI
                     RAAEMTLVAPDGKTETKLEPNVDFLPLFYTDAGSAEAGMVFAGWGISAPELNYDDYAG
                     LDVAGKFVLCFRGQPDRTDTRFYHYDEHRTRMKTAKDKGAVGLIYIYPSPISNPNGEW
                     QKDFFPAMISEKIGDAILNEIGSTSAELKKALTAFKRPIGFPLKSVIRMKVEADYHPD
                     GIGYNIAGYVEGSDPALRRDVLVIGGHFDHNGLHMGFLYPGADDNASGSATVMEIARA
                     FAGLARKPKRSVVFVLFGGEEMGLQGSIYYADHPPAVFPKIDAMFNYDMTGAGDGAWG
                     STTEENLRKVFLEADRHVGILRGMRVVQGPPGVRGSDQASFRIKGIPVASVGANGPSL
                     AYHETGDTIYRINPSIMGDIAKLSFLAAHAWADR"
     gene            complement(5173..5391)
                     /locus_tag="PKV64_09725"
     CDS             complement(5173..5391)
                     /locus_tag="PKV64_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17390.1"
                     /translation="MGRTIIALVHLGLFAGLVIYGFVLLAGGRINRGLTVLGLMGAYY
                     ALVLHKAVLAEIERKRRLRKENKTRSMK"
     gene            complement(5414..5977)
                     /locus_tag="PKV64_09730"
     CDS             complement(5414..5977)
                     /locus_tag="PKV64_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPN17391.1"
                     /translation="MLPAFLSLAGVFFAFALAAAEAAPAGPVAVVMKTSQGDITLELD
                     SEKAPRTVANFLSYVDAKFYDGTIFHRVIQNFMIQGGGHRPDMAQKVSKPPIKNESGN
                     GLTNARGTIAMAREAALHSATCQFYINTVDNFSLDQHQYCVFGRVVAGLDVVDAIAAV
                     PTSTQMGYDDVPRVPVVILSIRRAAAR"
     gene            6183..7724
                     /locus_tag="PKV64_09735"
     CDS             6183..7724
                     /locus_tag="PKV64_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPN17392.1"
                     /translation="MPRIKNLLVLPILASILAASAAAQYPGAAAAPLTDEQKLLEATR
                     LIRSETLFEYVKELTSETYGGRLTGTAEYDACADWVISLMKKWGVAPGGDDGTYLQKF
                     PNPYTLVFPGGTCVLHLPVKDGTVKKFYKFEEEFIPGGTSASGEVTAEVVFVGYGITA
                     PELGYDDYAGLDVKGKIVLMDPEVPVRPGDPAGAFPKWRPYSFHQYKLENAFDHGAKG
                     MIYNYGPIGNPNNSYREGFIYHHVGAAVVADVFAGTGRTYAETIARIRKELKPRSFAT
                     GKTMTIKNVTEHHPEGVGYNVIGVIPGTDPKLREEVILIGGHLDHLGRMWELMPGADD
                     NATAVAVGLGVAEAMAKSAIKPKRTVVFNFFGSEEQGVAGSKYYVEHPLFPLDKTLVF
                     INMEGPGGGDKISASGAKTYADFWAFIERANQKYVHRIIGGGEAAYPARPRQDSAWFF
                     WAGVPSLTFGAYGKNAPYPTYHNTKDRIELIDSETMEDMARLFFMAIMDIADEEAVNF
                     RVAEK"
     gene            complement(7746..8126)
                     /locus_tag="PKV64_09740"
     CDS             complement(7746..8126)
                     /locus_tag="PKV64_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17393.1"
                     /translation="MKFKSLMIIKSAVCLGFAPPMLFFPAWLLGLFGLAFGTGAALTA
                     REYGAALAGNMLLAWLARNVEEGRARKAICWNLCVYDAIGLIATLVLLFNKTLNPLGW
                     GVAAIYLFFAAGFGYFLLPRKKAA"
     gene            complement(8452..>8695)
                     /locus_tag="PKV64_09745"
     CDS             complement(8452..>8695)
                     /locus_tag="PKV64_09745"
                     /inference="COORDINATES: protein motif:HMM:NF014839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HPN17394.1"
                     /translation="IFDLLHVIRVYSKVPGKKAEFESPFILPRRSDVMRIASAVHKDF
                     VEKLAYARLWRGNEITGMMVNRDFALEDGDIVELHL"
BASE COUNT         1610 a         2673 c         2910 g         1502 t
ORIGIN      
        1 cgttggccga agtgggggag gcgctccaag ccctccacaa ggcttatccg ggcctgacga
       61 cgctggagga gatcggccgg agcgaggaag gccgcccgat ttgggccatg acggtcaaca
      121 atcccaagac cggtccggcc ctggccaagc cgggcgttta tgtcgacggc aacatccacg
      181 gcaacgaaat ccaaggcggc gagatcagcc tctaccttct cgactacctc ctgggacgct
      241 acgggaaaaa cgaggaggta acggccctcg tcgacaagaa ttgcttttac gtcgtccccg
      301 tggtcaacgt cgacggccga tatcatttcc tggccgatcc caacaacgcg agctccggcc
      361 ggggcctgcg catccccatc gacgatgacc gggacggcct cctcgacgag gatcctcccg
      421 acgatctgga caacgacggc aacatcaccc aaatgcgccg caaggacccc ttcgggcaat
      481 acaagacgaa tccggaggat ccccggctga cggtgaggat caagccgggg gaacggggcg
      541 aatggacgct cctcggcgaa gaggggatcg acaacgacgg cgacggccgg gtcaacgagg
      601 acgccgaagg ctacgtcgat cccaaccgcc aatggggctt cgactgggcc ccgtcctatg
      661 tcgagggcgg cgccggggaa tatcccttcc aaggccgggg cctccgggcg ctggccggct
      721 ggatgatgac caagaccaat atctgcctgg gctggtcgtt ccataatttc ggggggatgt
      781 tcctgcgcgg gccgacccgc aaggggctcg gcgactatcc cccgcaggac gtcgccgtct
      841 atgattacat cggccggcag gccgagcgga tcacccccgg ctaccggtac atggtcagtt
      901 ggaaagatct ctacaccacc tacggcgact cggtcgaatg gctggtccgc cacctcggcg
      961 cctacggcta ttgcggcgag gtcttccagg cctcggcgga gtccttcaag accggggagg
     1021 aagccgaacg aacgactccc cgcggggaag tggagcagat gctgcggggg gatttctggg
     1081 ccttggagcg gatcaagtac agcgacaatc tgaccatggg cgagatgtac aagccctggg
     1141 ccccgttcaa gcatccgacg tacggcgata tcgagatcgg cggatgggtc aagatgagca
     1201 accgcttgtc cgcgccgttc atgctgcccg acctcgtcca ccgaaacgcc atgtccgtcc
     1261 ttttctcggc caagcacacg cccgaagtga ctttggacgt gaccgaggtg aagaagatcg
     1321 gcgacggcct cttccgcgtc cggacccgct tggccaacgc caaggccgtc ccgtcgatga
     1381 gttttcaggc tcagaaggac cggttgtatc cccaggaccg gctgaccgtc tccgggccga
     1441 acgcccgggt catggccgga ggcgtcctcg tcgaccggga ctttgaccgg gtgagccaca
     1501 agaaggaccg gccggacgtc cagtttctcg tggttcccgg gttcggacgg gtggagcacc
     1561 agttcctggt cgaggggcgg ggcgaagtca ccctccgcta tgtctcccgg cacgccggga
     1621 tccgggaggt caggatctcg ctttgaggcg ccgcttgcgg gcgtagtagt ccttcaagat
     1681 ttcggcgtgg tcgaaggcca gggggaagtc aatctcccgg cgggtgaaga ttcccaggtt
     1741 cttggcgtcg tcccgggcgg ccggggtccc ctcggcccgg gcgatgaaca ccgtcgaaat
     1801 cgtgtggcgg cgcggatcgc gggccggatc ggaatacgtc cccagctgtt cgaccaggcg
     1861 gacgcggagc gacgtctcct cccaagcttc ccggaccgcg gccgtctcga gcgattcccc
     1921 gtattcgacg aatcctccgg gcagggccca gccgggcggg ggattcttcc gttcgatcag
     1981 gacgatgcct tcccggccgc gggcgtctcg gatctcgatg atgatgtcga ccgtcggaaa
     2041 cggattgcgg taccggcgag ggggacgacg ggattccatg acggcattgt aggccgggac
     2101 cgcggccggg gcaagaagcg gaacggaacg ccgacctcat cggggaatcg gggttcccga
     2161 aaaacagggc ggccgcctct tttcaccggc gtggccctct cccggccttc cacttccggg
     2221 gagaggcgcg gagaagtccg gcccgagcgt ctctccaagg cctctgaacc ggaacggaag
     2281 cggatcagcg gaaggatccg gtgatccgaa gcggcgcccc gagcacgacc cggagggcgg
     2341 ccgcccgctt ctggaaatcc ggcagccacc gggccggcag cggcgcgacc ggcatgaatt
     2401 tcgccgagag gggattgaga taggatccaa agcgcttgat ccggtaatca aggtgcggtc
     2461 ccgtggattc gcccgaggat ccgaccttgc cgatccgctg tcctccctcg accctctggc
     2521 cggcccgtac caggatcgtc gacaaatgca tgtaaatcgt ctcgtagccg tttttatggc
     2581 tcaagcggat catccggccg gccccgccct tccagccggc ttcctcgacc cggccgtcgg
     2641 ccgtggcgaa aacgggcgtg ccgaccgggg cggcgtaatc gacgccgtaa tgagcccgga
     2701 agacctttcg gaccgggtgg aggcggcggg aggaaaagcg cgaggtgatc cggtagctct
     2761 gcagcggcga cttgaggaac tccttgcgga gcgatcgccc gtcggggtgg aagtacccgc
     2821 tcttgaggct ccccccctcc tcgtagtcaa accggcaggc ctcgattctc cgcccgtcgt
     2881 tccaaaattc ggcggccagg atgtccccgt agcgctggaa gcggccgtcg aggtattttt
     2941 tttcgacgag aacgcggaag gcgtcgcccg tccgcagccc ggcgtaaaaa tcgatgtccc
     3001 agccgaaaat ctgctcaagc tcgatcgcca ggccggcctt ttcgcccccg gccaggaccg
     3061 cgccgtaaag atgctcgtcg atgacgccgt ggacgtagac ggggcgggtt tcgtaagcgt
     3121 aggctttttt ctcggcggca tacccctccc cggttcgggt caccaggagg aattggcttt
     3181 catcgatcgg gtaatccagg gacagaagct cgcccgaggg ggcgaacgtc atcttgaccg
     3241 tccgtccgga gacgatccgg gccaagttga aaaccggccg gacctggcgg accagggcgt
     3301 cgatttgagc gggcgagagg ccttcgtttc ctaaaatttc cgaaagcgtc tcgcctggaa
     3361 ggatgacttc ctcaaccgtc gtcgggcccg gctcggccgg ctggaggctc tcggccgagt
     3421 cgaccgccgg gacgatttcc cggcctggac cgaagggacg gaaaaccagg acggcggcgg
     3481 cgatcaggag gacgacgagg gcggccgcgg ccgcgacccg gagcggccgt ccggcgggcc
     3541 gctttttccg cggcggccgc cgcggagcca gcggattcgg accggaaagc ggcgggagaa
     3601 aaacgtcggg ttcgccgcgc ggcctctttc gtctcatgcc ggatcgagac ggccttcccg
     3661 gccggcgcgg ccggggggcc gtcctttcct agaacgaaaa ccggcggttc cgggtttcac
     3721 tttaccggtc ggcccaggca tgagcggcca ggaacgagag cttggcgata tcgcccataa
     3781 tggagggatt gatccggtaa atagtgtctc ccgtctcgtg gtaggccaaa gagggaccgt
     3841 tggccccgac cgaggcgacg gggatgccct tgatcctgaa ggaggcttga tcggagcccc
     3901 ggacgccggg cggtccttgg acgacgcgca tgccgcggag gatcccgacg tgccgatcgg
     3961 cttcgaggaa gaccttccgc aggttttcct cggtcgtgct gccccacgcc ccgtcgcccg
     4021 cgccggtcat gtcgtaattg aacatcgcgt cgatcttcgg gaagaccgcc ggcggatgat
     4081 cggcgtaata gatcgagccc tggaggccca tttcctcgcc gccgaacagg acgaagacga
     4141 ccgagcgctt gggtttgcgg gccagtccgg cgaaggcccg ggcgatctcc atcaccgtgg
     4201 cgctgccgct ggcgttgtcg tccgccccgg ggtaaaggaa gcccatgtgc aggccgttgt
     4261 ggtcgaaatg cccgccgatc acgaggacgt cccggcggag tgcgggatcg gagccttcga
     4321 cgtagccggc gatattgtac ccgatgccgt ccggatgata atcggcctcg actttcatcc
     4381 ggatcacgga tttgagggga aagccgatcg gccgcttaaa ggcggtcagg gctttcttga
     4441 gctcggcgct ggtgctcccg atttcgttga gaatcgcgtc gccgattttc tcgctgatca
     4501 tggccgggaa gaaatccttc tgccactcgc cattggggtt ggaaatgggc gaggggtaga
     4561 tgtagatcaa gccgacggcg cccttgtcct tggccgtctt catccgggtg cggtgctcgt
     4621 catagtggta gaaccgcgtg tcggtccggt cgggctggcc gcggaagcaa aggacgaatt
     4681 tgcccgcgac gtccaggccg gcgtaatcgt cgtaattcag ctcgggggcc gagatccccc
     4741 agccggcgaa caccatcccg gcctcggcgc ttcccgcgtc agtgtagaaa agcgggagaa
     4801 aatcgacgtt cggctcgagc ttcgtctcgg tttttccgtc cggggcgacc agcgtcatct
     4861 cggccgcgcg gatgaccgta tagggcgagg gatagggcag gagatagccg gtcttcggat
     4921 cgacgggctt gaggccccat tttttgaaca gcccggcggc ccagcgggcg gccgcggtgt
     4981 agccttcgtg gccggtgtag cggccggcga attcgggcga ggccatccgc tcgacatagg
     5041 cgtaggcctc cgagccgcgg atgccggcca gggctttggc cacgccggga tcgagcttgg
     5101 gcttgtcctg ggctccggcc gccagggcga cggcgagcat caggacggcg caaacgatcg
     5161 gtcgaagagt tttcatttca tgctccttgt cttgttttct tttctcaagc ggcgcttgcg
     5221 ttcgatctcg gccaggacgg ccttgtggag gacgagcgcg taataggcgc ccatgagacc
     5281 gaggacggtg agtccccggt tgatccggcc tccggcgagg aggacaaatc cgtaaatcac
     5341 caggccggcg aatagtccga ggtggacgag ggcgatgatc gtccggccca tgacggatga
     5401 tcctgaggcg tatctatcgg gcggccgccc gccggatcga aagaatgacc accgggacgc
     5461 gcgggacgtc gtcgtatccc atttgagtcg aggtcggaac cgcggcgatg gcgtccacga
     5521 cgtccaaacc ggcgacgacc cggccgaaga cgcagtattg atgctggtcc aagctgaagt
     5581 tgtccacggt gttgatgtag aactggcagg tggcgctgtg gagggccgct tcccgggcca
     5641 tggcgatggt tccgcgggcg ttggtcaatc cgttgccgga ttcgttttta atcggcggct
     5701 tggagacttt ctgggccatg tcgggccggt gccctccgcc ctggatcatg aaattctgaa
     5761 tgacccggtg aaaaatcgtc ccgtcgtaaa acttggcgtc gacataagaa aggaagttgg
     5821 cgacggtgcg gggggctttc tcagaatcga gctcgagggt gatatccccc tggctcgttt
     5881 tcatcacgac cgcgaccggt ccggccgggg cggcctcggc cgcggccagg gcgaaggcga
     5941 agaagacgcc ggccaggctg aggaacgcgg ggagcatcgt tctcatgtga acctcctcag
     6001 ggatcgggcc gccggcgccg gtccgaagga aagcggccgc cgccgggaat ccgaaaaacg
     6061 atgatagcgg atttaccggc ccggcgcaaa cgacccggcg aggaggcgat cccaggcgga
     6121 tcttttgcgc gcctccggcg cctcgtgata tcctggccgg gaatgcgcga aggaggtccg
     6181 tcatgccgcg tataaaaaat cttctggttc ttccgatcct cgcctcgatc ttggccgcat
     6241 ccgccgcggc ccaatatccg ggggcggccg ccgcgccgct cactgacgag cagaagctgc
     6301 tggaggcgac gcgcttgatc cgaagcgaga ccctgttcga atacgtcaag gagctgacgt
     6361 cggagacata cggcggccgc ttgaccggaa cggccgagta cgacgcctgc gccgattggg
     6421 tgatttcact catgaagaaa tggggcgtcg ccccgggcgg ggacgacggc acatatctcc
     6481 agaagttccc gaatccctac accctggtct ttccgggcgg gacgtgcgtc cttcatcttc
     6541 cggtcaagga cggaacggtc aaaaagttct ataagttcga ggaggagttc atccccggcg
     6601 gaacctcggc ctcgggcgag gtgacggccg aggtcgtctt cgtcggctac gggatcaccg
     6661 ccccggagct gggctacgac gattacgccg gcctggacgt caaggggaag atcgtcctga
     6721 tggatccgga ggtcccggtc cggccgggcg atccggccgg cgccttccca aaatggcggc
     6781 cgtactcctt ccaccagtac aagctcgaga acgccttcga ccacggggcc aagggaatga
     6841 tctacaacta cgggccgatc ggcaatccca acaactccta ccgcgaggga ttcatctacc
     6901 atcacgtcgg ggcggcggtc gtggccgacg tcttcgccgg gacgggacgg acgtacgccg
     6961 agacgatcgc ccggatccgc aaggagctca agccccgatc cttcgccacc ggcaagacga
     7021 tgacgatcaa gaacgtgacc gagcatcatc ccgagggcgt cgggtataac gtcatcgggg
     7081 tcatccccgg gaccgatccg aagctccggg aggaagtcat cctgatcggc ggacacctcg
     7141 accacctggg ccggatgtgg gagctcatgc cgggggccga cgataatgcc acggccgtgg
     7201 ccgtcggcct gggcgtggcc gaggccatgg ccaagtcggc gatcaagccg aagcggacgg
     7261 tcgtcttcaa tttcttcggc agcgaggagc agggcgtggc cggatcgaaa tattacgtgg
     7321 aacacccgtt gttccccctg gacaaaaccc tggtcttcat caacatggag gggccgggcg
     7381 gcggggacaa gatctccgcc tcgggggcca agacgtacgc ggatttctgg gcgttcatcg
     7441 agcgggccaa ccagaagtac gtccaccgga tcatcggcgg cggcgaggcg gcgtacccgg
     7501 cccgtccgcg gcaggattcg gcctggttct tctgggccgg cgtcccgtcc ctgaccttcg
     7561 gggcctacgg caagaacgcg ccgtacccga cgtatcacaa cacgaaggac cggattgagc
     7621 tgatcgactc cgagacgatg gaggacatgg cccggctgtt cttcatggcg atcatggaca
     7681 tcgccgacga ggaagccgtc aacttccgcg tcgccgaaaa atagggacgg ggttttcttt
     7741 ttttgtcagg cggctttttt ccgagggaga aggaaatagc cgaagccggc ggcgaagaac
     7801 aagtagatcg ccgcgacccc ccagccgagc ggattcagcg tcttgttgaa gagcaagacc
     7861 agggtggcga tgaggccgat cgcgtcgtag acgcagaggt tccagcagat cgccttccgg
     7921 gcccgccctt cctcgacatt ccgggccagc caagccagga gcatgttgcc ggccaaggcc
     7981 gccccgtatt cccgggcggt cagggccgcg cccgttccga acgccagccc gaaaagcccg
     8041 agcagccagg ccgggaaaaa aagcatcggc ggcgcgaatc ccaggcagac ggcggacttg
     8101 atgatcatca ggcttttgaa cttcatgttt tcctccttat cctttatggc gaggacggtc
     8161 attcgcacgg aaggcgacgg cttaaggaag ggaaaaaggc cgaaccgggc gggaaaatca
     8221 aggctggccg acgatccccc ggcttgaggt catcatcagt caaatcctct tttaaagcag
     8281 aaagtgatcc cctcggcccg gagggagaac cccttctcct gagaagagag ggtttggctc
     8341 ttcgaaagcc cgccgcccgg atcttttccc ttgtctgctg ataaaaatag ggcgggaatc
     8401 agggaatgtc aaccggcgga aagcatcgag gccgatcggc ccgttttctt tttagaggtg
     8461 gagctcgacg atgtcgccgt cctcgagggc gaagtcgcgg ttgaccatca tcccggtgat
     8521 ttcgtttccc cgccagaggc gggcgtaggc gagcttctcg acgaaatcct tgtggacggc
     8581 cgaggcgatc cgcatgacgt cgctccgccg ggggaggatg aacggcgact cgaactcggc
     8641 cttcttgccg gggaccttgc tgtagacccg gatgacgtgg agcaggtcga aaatc
//
LOCUS       DAOOXH010000097         8645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_9020_length_8645_cov_8.125960, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000097 DAOOXH010000000
VERSION     DAOOXH010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9020_length_8645_cov_8.125960"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PKV64_09750"
     CDS             <1..754
                     /locus_tag="PKV64_09750"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPN17395.1"
                     /translation="VTVVGQVQAVIPNPAGTTRGTYTMLDESCPNPLTIRTNDLPPVS
                     RNFRVRGVLIQDPAQANVPLMKEISRSSPGMTNTTLYLLIAAGAAFLFLLIVFIILLT
                     KPKPRPAAEETIRPAAPVDPSKTTRIAPPPAAPAAAPGGAKTRVFLNLGADIIIEKGP
                     DKGKEFPLHRQVTTIGRPGSRKNEIEINDDTVSKEQASIYYDNAKKEFTIADESATNP
                     TRVDGRAISGPTPLKNGAVIELGRTVLIFKKS"
     gene            776..1600
                     /locus_tag="PKV64_09755"
     CDS             776..1600
                     /locus_tag="PKV64_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HPN17396.1"
                     /translation="MYPRIEAFGQSDAGCVREVNEDNFLCLDLSTAPAGSEPAAFLLA
                     VADGIGGHAGGAVASAMAVQILREEIERVDGVDPRSYLDDVFRKANRLIFERASREPV
                     YAGMGTTLVAAIGKGERLFLANVGDSRAYLFRDGDLRQLTEDHSWAGEQRRRRLLSEQ
                     EIRQSPFRSMITRSLGYQQNVIVDTFDVEACGDDVFLFCTDGLYGLVMEKRMSRVLRK
                     HPDPKAACAALIAEAKAAGGDDNITVVVASFKEPGDAPAVRPYDTVRIPPFPEEKP"
     gene            1823..2206
                     /locus_tag="PKV64_09760"
     CDS             1823..2206
                     /locus_tag="PKV64_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17397.1"
                     /translation="MEISAKRILGSDANNAPPLPAGMSRPETGKATDRSPAGAVPSVP
                     AEMVPSTPASAVQVPLQATPARKLAQASEALNRGGYRDALDAAEALLAENRGLDAAKT
                     VALDAVIRLATGGIKDLVGLIRPEL"
     gene            complement(2466..3248)
                     /locus_tag="PKV64_09765"
     CDS             complement(2466..3248)
                     /locus_tag="PKV64_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN17398.1"
                     /translation="MAEFLALRDIVKIYGTTARTTALRGVSLSLAAGEYAALIGRSGS
                     GKSTLLNIIGTLDRPTSGEMRVDGRDIFALDDDGLAAFRGRTIGFVFQFHHLLPEFTA
                     LENVLLPFRAAHGRTTAAASVRARRLLERVGVGDRMNHKSNALSGGQQQRVAVARALI
                     NEPRLILADEPTGNLDTESSEAIKALLREINRESGTTFLIVTHDRHIAAGCDRVVEID
                     DGGIVRDFRPPETTADEAWAALAPCECRERVRGDGGPAPTRP"
     gene            complement(3241..4455)
                     /locus_tag="PKV64_09770"
     CDS             complement(3241..4455)
                     /locus_tag="PKV64_09770"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN17399.1"
                     /translation="MFDVFVAFRFLRESRAQTFLILGGIVLGIAVQVFLNALISGLQA
                     DLIRKTVGHAPHIIGTTPDAEPVAVLPSDPREKTIVRIVTNEGGVKVIRDGAVIGSQI
                     EKLGAFTVILPVARGSGYVWQGQKILPVILRGADLETADRLYDIRRRLTAGRYEVGPD
                     TVLIGKDLAAKLRLEAGSVLRLSAAGGGEDLFRVGGIFDLENQVLNETWILLSLARAQ
                     NLIGLDRGLSEIELQTKDVFEAERRAAELRAVFPSVRWRSWEETNASLLSALRSQSSS
                     STLIQLFVLLSVTLGIAGVLAVSVIQKSRQIGILKAIGTMTRHVGRIFVIQGAVLGFL
                     GSVLGVLAGAGLIFLFQGISGGSPSAFPFSVSFGAAAVSVAAATAAGTLAAFIPARRA
                     SRLSPIEVIRNG"
     gene            complement(4459..5670)
                     /locus_tag="PKV64_09775"
     CDS             complement(4459..5670)
                     /locus_tag="PKV64_09775"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPN17400.1"
                     /translation="MKPTGPALSKVLLWLAAAAAVAALVILPRIFKSTPAWSTTVATQ
                     DALETVLASGRVVGEKSVPLSFVRPGRIAAEFIRDGSRIAAHSVLMRQDTRPLEQALA
                     QAQTALAEARLKKDKLLSVDLADAEEKVRQAKANNAYAADFFSRQTELFAQKSVSLLQ
                     YEQSRRDRDLAASALAAAENQLRSLKEVQTVQADLAISKTENDLKKAELDLDDTILTA
                     PAAGMVVSHDAHAGEFVQAGQRVALFIPEAPRTSIEIQVDETNAGRIAPGQPASVTSP
                     AYPGRVFTGKVERLGAIVDAQRGSFPIVIALDRFEPDLLPESSVSAQITTGRATDVLL
                     LEQRFILREGEKASVYIEENGRAKKVPVSVRDLGSGLFECREGLKSGQSVLLPQGLKD
                     GLKIKLKPLPE"
     gene            5804..6985
                     /gene="truD"
                     /locus_tag="PKV64_09780"
     CDS             5804..6985
                     /gene="truD"
                     /locus_tag="PKV64_09780"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HPN17401.1"
                     /translation="MIKAKPGDFVVEERADLPLEPGGRYAVYRLHKSGWNTSDLVGHL
                     ARRFGFPPGRIAYGGKKDKHGLTSQFITIEDAADRSLEGKDFRLEAAGRMNRPMGPDL
                     IRANDFTVTLRDLAGAESLEPAVGRIKTEGFPNWFDDQRFRSYDPERGFFAEKILRRH
                     WNGALQVYLTSVTPGLAGRERTRRLELFRVWRDWKACRSMAEGPFEREVFDILRKEPA
                     AFGEALHRIPEEETAMQYAVFQAHLWNETLRKIFRSRGLPVVEIAGREGPYLFWRKLG
                     KADRAALHALRIPTAAAKMEFPDPEAEAPFAAVLADKGLARSDFRTRELRRVRFQSFL
                     RTAAVVPEGFTVLESGADELHPGREKIIFRFSLPRGSYATMLIKTITLAGNPPRRRTA
                     G"
     gene            7020..7379
                     /locus_tag="PKV64_09785"
     CDS             7020..7379
                     /locus_tag="PKV64_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPN17402.1"
                     /translation="MDYATLFRGFPGAVTVCDREGVILAMNDRAIETFAGGGGDRLLG
                     TSVFSCHPEPARTKLRALLAEGRTNVYTIEKNGVRKLIYQAPWKENGAYAGFLELSLV
                     LPAAMPHFVRGSQEDLP"
     gene            7517..8617
                     /locus_tag="PKV64_09790"
     CDS             7517..8617
                     /locus_tag="PKV64_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPN17403.1"
                     /translation="MPEKSVCFCAYPSAFIYNPADLSDEKNRAKHLFWGDTVVREGGP
                     QNGWVRVSSRDVSGWMRESDVQSDPLLDIIFLDVGQGDSCLVVTPADEKIIVDAGKED
                     NLLRYLRWRYKNYETAEMRAMGGRIKALVISHPDDDHYGGLRGILDDPKFGSLIDFEA
                     VYHNGLFPRAGSGLDSLGARTKVGTIGYLEEPIVTQADLDDFFAHAAGWQSLEYPSLV
                     HRLRMKNPATTVRMIHHGAPGIANLTAVPGLSVTVLGPVLEPGGSRGRLRTFSGSVPM
                     TKNGHSVVLRIHYGSVSILLGGDLNIPSEKFLLDFYKDKPGIFRSDVLKAYHHGSGDF
                     AEEFIEAVCPYVSIISSGDNESYSQCLLYCLN"
BASE COUNT         1581 a         2706 c         2814 g         1544 t
ORIGIN      
        1 ggtgaccgtc gtcggccagg ttcaggccgt gatccccaat ccggccggaa cgacccgggg
       61 aacgtacacg atgctggacg aaagctgccc caatccgctg acgatccgga ccaacgacct
      121 cccccccgtc agccgtaatt tccgggtgag gggagtcctc atccaggatc cggcccaggc
      181 caacgttcct ctcatgaagg agatttcccg gtcctcgccc gggatgacca atacgaccct
      241 ctacctgttg atcgccgcgg gcgcggcctt tctctttctc ttgatcgtct tcattattct
      301 cctgacgaag cccaagccgc gtccggccgc cgaggaaacc atccggccgg cggccccggt
      361 cgatccatct aaaacgaccc ggatcgctcc tcccccggcg gcgcccgcgg ccgcgccggg
      421 gggggccaag acccgggtgt ttctcaattt gggagccgac atcatcatcg aaaagggtcc
      481 ggacaagggg aaggagttcc ccctccaccg gcaggtgacg acgatcggcc gtcccgggag
      541 ccggaaaaac gagatcgaaa tcaatgacga caccgtctcc aaggagcagg cctcgatcta
      601 ttatgacaac gcgaagaaag aattcaccat cgccgacgaa agcgcgacga atccgacccg
      661 ggtcgacggc cgggcgatca gcggcccgac gccgttgaaa aacggggccg tgatcgagct
      721 gggtcgcacc gtcctgatat tcaagaagag ctgaggcgtt ccgaaacgag gcccggtgta
      781 tccgagaatc gaagcgttcg ggcaaagcga cgccggctgt gtccgggaag tcaacgagga
      841 caatttcctt tgcttggacc tgtccacggc gccggccggg tcggaaccgg cggcgtttct
      901 cttggccgtg gccgacggca tcgggggcca cgccggcgga gcggtcgcca gcgccatggc
      961 cgtccagatc ctgagggagg agatcgagcg cgtggacggc gtcgatccca ggtcgtatct
     1021 ggacgatgtc ttccggaaag ccaaccggct catcttcgaa cgggcttccc gggagccggt
     1081 ttacgccggg atggggacca cgctggtggc cgcgatcggg aaaggggagc gcctcttctt
     1141 ggccaacgtc ggcgacagcc gggcttatct cttccgcgac ggggatctcc gccagctgac
     1201 cgaagaccat tcctgggccg gggaacagcg gcgccgccgt cttctcagcg aacaggaaat
     1261 ccggcaatcg ccgttccgct cgatgatcac ccggtcgctg ggatatcaac aaaacgtgat
     1321 cgtggacacg ttcgacgtgg aagcctgtgg ggacgatgtt ttccttttct gcacggacgg
     1381 cctctacggc ctggtaatgg aaaaacggat gtcccgggtt ttaagaaaac acccggaccc
     1441 caaggcggcc tgtgccgcct tgatcgccga ggccaaggcc gccggcgggg acgacaacat
     1501 cacggtcgtc gtcgcttcgt tcaaggaacc gggcgacgct ccggccgtcc ggccctacga
     1561 cactgtccgg attcctccgt ttccggagga gaagccgtga agatcggcgg catcccggcc
     1621 gctgtttcgt gaatgaccat gaatccctcc cggcctccgg gatccgctgg accctcgccc
     1681 aggtcgtcgc cttcgccttc ctcccgacgg aagccgcttc ttctcgccgt cgggctcatg
     1741 atggggcttg tcgccgtcct cgccgcgatc ctccttcgtt cgtcccggaa acaagccccg
     1801 gtcgccgccc gggcggctca aagtggagat ttccgcaaag agaatcctcg gaagcgacgc
     1861 gaataacgct ccgccgcttc cagccgggat gtcccggccg gagacgggaa aagcgaccga
     1921 ccgatcgccg gccggagccg ttccgtccgt tccggccgag atggttcctt cgacaccagc
     1981 gtcggccgtt caggttccgc ttcaggcgac tccggcccgg aagctcgctc aggcctcgga
     2041 agccctgaac cggggggggt atcgcgacgc cctggacgcg gccgaagcgc tcctggctga
     2101 gaatcgggga ttggacgcgg ccaaaacggt cgccctggac gcggtcatcc gcttggctac
     2161 ggggggaatc aaggacttgg tcggattaat acgccctgag ctataaaatc aagcacccgg
     2221 cgggattctt ccgggatcat gccgcgccgg acgtctatca gcgccttcgg cccgacctgg
     2281 aaatgatgac aagcgcctcc cttgacatcc aaatcgtgat ttccaacctg aagctcgatt
     2341 tccaggcggc ccgctatccg gcgtacgcga cccgggccgt gttcgcccag gtcatgacgg
     2401 ggatatcggc ggccgttttt cctggatcct ggaacgacgc ggcggcgagg ggacgatcac
     2461 gtccgtcaag gtcgagtagg cgccggcccc ccatcgcctc gaactcgctc ccggcattcg
     2521 cagggagcca gggcggccca agcttcgtcc gccgtcgttt ccggcggccg gaaatcacgg
     2581 acgatccccc cgtcgtcgat ttcgacgacc cggtcgcagc cggcggcgat atgtcggtcg
     2641 tgggtgacga tcaaaaacgt cgtcccgctt tcccggttga tctcccgcaa caacgccttg
     2701 atggcctcgc tcgattcggt gtcgagattt ccggtcggct cgtcggccag gatgagacgc
     2761 ggttcattga tgagggcccg ggcgacggcc acccgctgtt gctgtccgcc ggacagggcg
     2821 tttgatttat ggttcatccg gtcgccgacg ccgacgcgct cgaggaggcg gcgggcccgg
     2881 acggaggccg cggcggtcgt ccgcccgtga gccgcccgga agggcaggag aacgttttcc
     2941 aaggcggtga actcggggag gagatgatgg aactggaaga cgaacccgat cgtccggccg
     3001 cggaaagccg ccaggccgtc gtcatcgaga gcgaagatgt cccggccgtc gacccgcatc
     3061 tctcccgagg tcggccggtc gagcgtcccg atgatgttga ggagcgtcga ctttccggac
     3121 ccggaccggc cgatgagggc cgcgtattcg cccgccgcca gggaaaggct cacgccccgg
     3181 agggcggtcg tccgggccgt cgtcccgtag attttaacga tgtcgcggag ggcgaggaac
     3241 tcagccattg cggatcacct cgatcggact gagacgggag gcccgccggg ccggaatgaa
     3301 ggccgccagc gttccggccg cggtcgccgc ggcgacggag acggcggccg cgccgaacga
     3361 gacggagaaa gggaaggcgg agggggagcc gccgctgatg ccctggaaaa ggaaaatgag
     3421 cccggccccg gccaggacgc cgagaaccga gccgagaaaa cccaggacgg cgccctggat
     3481 gacgaagatc cttccgacat ggcgggtcat cgtcccgatc gccttaagga tcccgatctg
     3541 acgggatttc tggatcaccg aaacggccaa aacgccggcg atgcccaggg tgacggacaa
     3601 caggacgaac agttgaatca gggtggaaga cgagctctgg ctccgaaggg cggaaaggag
     3661 cgaggcgttg gtctcttccc agctccgcca acggaccgag gggaaaaccg cccgaagctc
     3721 ggccgcccgc cgctcggctt cgaacacgtc cttcgtctgg agctcgattt cggacaagcc
     3781 ccggtcgagc ccgatgagat tctgagcccg ggccagggag aggagaatcc aagtttcgtt
     3841 gagaacttgg ttttccaggt cgaagatccc gccgacccgg aagaggtcct cgccgccgcc
     3901 ggccgcggac aggcgaagga ccgagccggc ctcgaggcga agcttggcgg ccaggtcctt
     3961 tccgatcagg accgtgtccg ggccgacttc gtaccggccg gcggtcagtc tgcgccggat
     4021 atcgtaaagc cggtccgcgg tctcgagatc cgcgccccgg agaatcaccg ggaggatttt
     4081 ttgcccttgc cagacatatc ccgatccccg ggcgacgggg aggatgaccg tgaaggcgcc
     4141 gagcttttca atctggcttc cgatgacggc cccgtcccga atcaccttga cgccgccttc
     4201 gttggtcacg atgcggacga tcgttttttc ccgcgggtcg gacggcagaa cggcgacggg
     4261 ctcggcgtcg ggcgtcgttc cgatgatgtg cggcgcatgg ccgaccgtct ttcggatgag
     4321 atcggcctgc aatccggaga tgagggcgtt gagaaagacc tggacggcga tgccgaggac
     4381 gatgccgccc aggatcagga acgtttgggc ccggctttcc cgaaggaagc gaaaagcgac
     4441 gaaaacgtcg aacatggcct attcggggag cggtttgagc ttgatcttca gcccgtcttt
     4501 cagcccttgc ggaagcagaa cggattgacc gcttttcaat ccttcccggc attcgaataa
     4561 gccgctgccc aggtcgcgga ccgagacggg aacctttttg gctctcccgt tttcctcgat
     4621 gtagacggag gccttctctc cttcgcggag gatgaaccgc tgctcgagaa gcaggacgtc
     4681 ggtcgcccgg ccggtcgtga tctgggccga tacggatgat tcgggcaaca aatccggctc
     4741 gaaccggtcc agggcgatga cgatgggaaa agagccgcgc tgggcgtcga cgatggctcc
     4801 cagccgttcg actttcccgg tgaagacgcg gccgggatac gccggcgaag tgacggaggc
     4861 cggttgtccc ggagcgatcc ggccggcgtt ggtttcgtcg acctggattt cgatggacgt
     4921 ccggggcgct tccgggatga acagggcgac gcgttggccg gcctggacga attcgcccgc
     4981 gtgggcgtcg tgggaaacga ccatgccggc cgccggagcg gtgagaatcg tgtcgtcgag
     5041 gtcgagctcg gctttcttga ggtcgttttc cgtcttggaa atcgccaagt ccgcctggac
     5101 ggtctgcact tctttcagcg accggagttg gttttcggcc gcggccaagg cggaagcggc
     5161 caaatcccgg tccctccggc tttgttcgta ctgaagaagg gagacggact tttgagcgaa
     5221 aagctcggtt tggcggctga aaaaatcggc ggcgtaggcg ttgttggctt tggcctggcg
     5281 gaccttttcc tcggcgtcgg ccaggtcgac ggagagaagc ttgtctttct tgagccgggc
     5341 ttcggccaag gcggtctggg cctgggccag ggcttgttcc agcggacggg tgtcctggcg
     5401 catgagcacg gaatgggccg cgattcggct gccgtcccgg atgaattcgg cggcgatcct
     5461 ccccggccgg acgaaggaaa ggggaacgga tttttccccg acgacccggc ccgaggccag
     5521 gacggtttcc agggcgtctt gggtcgcgac cgtcgtcgac caagcgggcg tggatttgaa
     5581 aatccgcgga agaatgacga gggcggcgac ggccgcggcc gccgcaagcc agaggagaac
     5641 cttgctgagg gcggggccgg tgggcttcat cggaacatac ctttcttaat ggaggcgcaa
     5701 ttcccgtcgg aaagacggca tcttaaccga atccgaagcg ggaatcaacc gacgatttcg
     5761 tccgccggcg gggaaaggcg aaggccgtgt tataatccgg gccgtgatca aggccaagcc
     5821 cggagatttc gtcgtcgagg agagggccga tctcccgctc gagccgggag gccgctacgc
     5881 cgtctaccgg ctccataaaa gcggctggaa cacctcggat cttgtcggcc atttggcccg
     5941 ccggttcgga tttcctcccg ggcggatcgc ttacgggggg aaaaaagaca agcacggcct
     6001 gacgtcccag ttcatcacca tcgaggacgc ggccgaccgg agcctcgagg gaaaggactt
     6061 ccggctcgag gcggccggcc ggatgaaccg gccgatgggg ccggacttga tccgggccaa
     6121 cgatttcacc gtcaccctcc gggacctggc cggcgccgag tccttggagc cggctgtcgg
     6181 gcggatcaag acggaagggt ttcccaactg gttcgacgac cagcggttcc ggagctacga
     6241 tcccgagcgg ggttttttcg ccgagaagat cctccgccgg cactggaacg gggctctcca
     6301 ggtctacctg acctcggtca ctccgggact tgccggccgg gagcggaccc ggcggctcga
     6361 gttgttccgc gtctggcggg actggaaggc ctgccggtcg atggccgaag gacccttcga
     6421 gcgggaagtg ttcgacattc tccggaaaga accggccgcc ttcggcgaag ccctgcaccg
     6481 catcccggag gaggaaacgg ccatgcagta cgccgttttc caggctcatc tctggaacga
     6541 aaccctccgg aaaatcttcc ggagccgcgg cctcccggtc gttgaaatcg ccggccgcga
     6601 aggcccctat ctcttctggc ggaagctcgg caaggccgac cgggccgctc tccatgccct
     6661 ccgcatccct acggccgcgg cgaaaatgga gtttcccgac ccggaggcgg aggcgccgtt
     6721 cgccgccgtg ctggcggata aaggcttggc ccggtccgat ttccggacgc gggagctgcg
     6781 gcgggtccgc ttccagtcat ttctccggac ggcggccgtt gtccccgaag gcttcacggt
     6841 cctcgagtcc ggcgccgacg agctccatcc gggccgggaa aaaatcattt tccggttctc
     6901 cctgccccgg ggatcctatg cgacaatgct catcaaaacg atcaccctcg cgggaaatcc
     6961 gccgcggcgg agaaccgccg gatgaccggg ttcgaggacg attatggagg aggcgcttga
     7021 tggattacgc gacattgttc cgaggctttc ccggcgcggt cacggtgtgc gaccgagagg
     7081 gcgtcatttt agcgatgaac gaccgggcga tcgagacgtt cgccggcggc ggcggagacc
     7141 gccttctcgg aacgagcgtg ttttcatgcc accccgagcc cgcccggacg aagctgcggg
     7201 cgcttctggc cgagggccgt acgaatgtct acacgatcga gaaaaacggc gtccggaaat
     7261 tgatctacca agcgccgtgg aaggaaaacg gcgcttatgc cggattcctc gagctttcgc
     7321 tcgtcctccc ggcggcgatg ccccatttcg tccgggggag tcaagaagac ctgccgtgaa
     7381 cggcccggcg acgcgtcttt tccgggagta aggcgttccc gcgggccgga gatttgattt
     7441 ttcccttctc cggatttaca ttcctgttaa atcatcggga acggggagac gttcatccgt
     7501 ttgaaggagg gattccgtgc ctgaaaaatc cgtttgtttc tgcgcgtatc catcagcttt
     7561 catttacaat cccgcggacc tctcggacga aaagaaccgg gcgaaacacc tgttctgggg
     7621 agacacggtc gtccgcgaag gcgggcctca aaacggctgg gtccgggttt cgagccgcga
     7681 cgtctccggc tggatgcgcg aatccgacgt gcagtccgat ccgcttctgg acatcatctt
     7741 tctcgacgtc ggccagggcg acagctgcct ggtcgtcacc ccggcggacg aaaaaatcat
     7801 cgtcgatgcc ggcaaagaag acaacctcct ccgctatctc cgctggcgat acaagaacta
     7861 cgagacggcg gagatgcggg cgatgggcgg ccggatcaaa gccctggtca tctcccatcc
     7921 ggacgacgat cactatgggg ggcttcgcgg cattctcgac gatccgaaat tcggttcgct
     7981 catcgatttc gaagccgtct atcacaacgg ccttttcccg cgggcgggga gcggattgga
     8041 ctccctgggc gcccggacca aggttggaac gatcggctat ctcgaggagc cgatcgtgac
     8101 ccaggccgat cttgacgatt tcttcgccca tgcggccggc tggcaaagcc tggaataccc
     8161 ctccctggtc catcgcctcc ggatgaaaaa cccggcgacg accgtccgga tgattcatca
     8221 cggtgcgccc gggatcgcga atctcacggc tgttccggga ctatcggtca cggtcttggg
     8281 tcccgtgctt gaaccgggcg gttcgcgcgg acgtctccgg acgttctctg gaagcgtccc
     8341 gatgaccaaa aacggccatt ccgtcgtgct ccgcatccac tatgggagcg tctcgatcct
     8401 cctaggcggc gacttgaaca tcccgtccga gaaattcctg cttgatttct ataaagacaa
     8461 gccggggatc ttccggtccg acgtcctcaa agcctatcac cacggcagcg gcgacttcgc
     8521 cgaggagttc atcgaggccg tttgccccta tgtctcgatc atctccagcg gggacaacga
     8581 atcctactcc caatgtctgt tgtattgttt gaactgaatt tatatttaga cgttcttata
     8641 cgtgt
//
LOCUS       DAOOXH010000098         8398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_9389_length_8398_cov_6.756323, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000098 DAOOXH010000000
VERSION     DAOOXH010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9389_length_8398_cov_6.756323"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1953)
                     /locus_tag="PKV64_09795"
     CDS             complement(<1..1953)
                     /locus_tag="PKV64_09795"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPN17404.1"
                     /translation="MYIKRLELQGFKTFPDRTKILFNPGITIIIGPNGTGKSNIVEAI
                     QWVLGGQRVRTVRGEKIDDAVFAGTALRPPMGMADVSLVLQNSEDEITVNHRVFRTGE
                     SEYRLDGKIVRLKDIQDELWKRTISENRYFVIEQGAIGTFVTSKPVEKRALIEEAAGT
                     AFFKDKKRQAENKLEDSERNLVRLEDIIAEVSKAKNSLARQAGAAERYRRLRERVREL
                     NVLHFRLKSAQLAAGQADILARYEESSIREREQQARLADEEGAVNQKRKNVWELEQAF
                     TGGRENLYTIRSQIARLEAERERDAKRSEFIEESRRKAAADADERLRELLGLEEEGAR
                     SREEIQALTAALVVKKEAIVSFERTLAETTARISPLLEQSDRLRSDHLQKLAEWTAAR
                     NDAAKVEKELEMLTRQEEKLKTRKEEARALVEAKSEDLRGLESRRAASLEESGAVRGE
                     WTALRGRLAETAETVAGLEHRLQELRALRDADIHHLQALEKIAVKERASDETSSVDGA
                     LGLLAELVETEAADAALVDVFWKDESRAAIIAPEDLLRGLDGRTLKGRYLLLGGTAGD
                     PDGGEWRDQPGVLGTLKSRLRPGSRLPGGLPALPDAVIVEDVRTAVRLWLERPGANFI
                     TRGGDVLSAAGLLSLGPRREGLFGLHQ"
     gene            2054..2422
                     /locus_tag="PKV64_09800"
     CDS             2054..2422
                     /locus_tag="PKV64_09800"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPN17405.1"
                     /translation="MEDDKRKYFRFECLIPVDLVETGDPDAKPEEALIGNVSREGMRL
                     VFDLGRAFQEGDKVRFQIQTPEDNRVCRMTGEVIWSRSQGEKIEVGLRLQETEKCSKS
                     ELLDLGYEAWRKHQKEIETK"
     gene            2681..2753
                     /locus_tag="PKV64_09805"
     tRNA            2681..2753
                     /locus_tag="PKV64_09805"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2714..2716,aa:Thr,seq:cgt)
     gene            2835..3359
                     /locus_tag="PKV64_09810"
     CDS             2835..3359
                     /locus_tag="PKV64_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPN17406.1"
                     /translation="MADFDFSSRKPEIHPDTFIAEGARLVGRVVLAAGSSVWYNCVLR
                     ADVGDIIIGEKTNIQDNSTIHVDFDLPAVIGDHVTVGHGAILHACTIGSHCLIGMGAV
                     ILDKAVIPPRSIVGAGALIPPRKTYPEGSLILGSPAIVARKLTAEEIERLPEHAREYV
                     LLWKAIAGRGIGGI"
     gene            3434..4600
                     /locus_tag="PKV64_09815"
     CDS             3434..4600
                     /locus_tag="PKV64_09815"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPN17407.1"
                     /translation="MQAILPHKIRKAREAAHLNQGAFARAVGLSSEYISLLEAGKRTP
                     SMATLEKIAGFLNRDLSYFFQEKPAPPDPFALLFRAEAVDEKTRAELQRFRRYCDDYV
                     RLENLTGRRLETAPFYSGLSPERMADEERRRLGLGDEPIRDVFALLETNGCRLYRMPL
                     PEDSKVSGVFLYLEQKEAAFALINSGGSPGRQVFHAAHEYCHYLKDRFDGPVIDGPDV
                     FIDEYASLYHPREPFAQAFAARFLMPPGKIKEIIEKDFRASRLAYDHVLYLKRYFGVG
                     ASAMLRTLRTMSLVSAAQYDEFVRIDPGSREKDLFGRASGEDPVAGGPGTAPGGEGEE
                     RIEAGAASGTAGPPARIKRKPVPSDRYRMLQKEAIRKIARDRELRKAVQGTLPT"
     gene            4626..5066
                     /locus_tag="PKV64_09820"
     CDS             4626..5066
                     /locus_tag="PKV64_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17408.1"
                     /translation="MGGIKWSSAVLFLFAATLLTAAFSCSSSTGGKGTGGAEWVMMDE
                     SYVPKDDVEAFIKADAVNRGALPLFIRNHKSDKRALSGFKGRQYAGPSPRTLEMLNPG
                     LDDWMLVDIRYKSESEREIVRTVLYLSIRGVWKVGDSGTLIASS"
     gene            complement(5114..6688)
                     /locus_tag="PKV64_09825"
     CDS             complement(5114..6688)
                     /locus_tag="PKV64_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPN17409.1"
                     /translation="MDASGPIEINDQFRQALHWMEETARPVFVTGKAGTGKSTLLEHF
                     RETTLKKIAVLAPTGVAALNVRGQTIHSFCGFKPDITLVKVRKINAKKDPERAALLRK
                     LDAVVIDEISMVRADLLDCVEKFLRLNGPKPRRPFGGLQLILIGDLYQLPPVVAGVEK
                     TLFTLHYETPYFFSAHCLLRDSFRLEFVELEKIYRQTDAGFIALLNAVRNRSAGPEDL
                     EKLHSRYDPEFVPPEDDFYVTLTSTNDLAAARNREKLALLPGRLYAYEAIVEGEFERS
                     SLPTDEHLEIKAGAQVMLLNNDAAGRWVNGSIGRVAGVLREKGRDDRIAVDLQDGKRV
                     EVAPNEWDLYRFAYDSEKDAVVSEAVGAFIQYPVKLAWAITIHKSQGKTFRNVIVDVG
                     RGTFAHGQVYVALSRCTSFEGLVLKTPILRSHIRTDWRVSRFLTRFQYDRADERQPYI
                     EKKRLIEEAVRDGRDLEILYLKPDDTKSRRRIRPEAVEEMEYGGKAFEGVRAYCHERE
                     DMRTFRIDRMLEVRIC"
     gene            complement(6795..7814)
                     /locus_tag="PKV64_09830"
     CDS             complement(6795..7814)
                     /locus_tag="PKV64_09830"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HPN17410.1"
                     /translation="MSPKGKAAIAVLSLLLGWTAWKGVHFVSSSPRVHISVPADSRES
                     APMKKIVQKSPEEWKKALTPAQYQVLIACGTEPPFSGIYDHFWESGGYHCAACGALLF
                     RSETKYEHGTGWPSFFESASPGALERREDRSAGMIRTEIRCAACGGHLGHVFDDGPPP
                     TGRHYCVNSAALAFRPSAAAPAGGPETATFAAGCFWGVEEKFGRLPGVLETVVGYTGG
                     RTKNPTYEQVCTDTTGHAEAVRVTFDPARISYAGLVQAFFSFHDPTQLNRQGPDVGRQ
                     YRSAVFYHTEEQRKQAEEIKAEIDRSGRLPGRLVTEIVPAGPFTRAEEYHQKYNLKNK
                     RACSF"
     gene            complement(7914..8246)
                     /gene="trxA"
                     /locus_tag="PKV64_09835"
     CDS             complement(7914..8246)
                     /gene="trxA"
                     /locus_tag="PKV64_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPN17411.1"
                     /translation="MKNVPETSEARFEAEVLKAPRPVVVDFWAPWCGPCRMMAPVLEE
                     IAGKFDGEVDFRKLNTDENESTARTHGIMAIPTLIVFREGREAERIVGLAPASEIESK
                     IRSVLTRS"
BASE COUNT         1464 a         2565 c         2736 g         1633 t
ORIGIN      
        1 ctgatgcagg ccgaacagcc cctcccggcg gggaccgaga gacaagaggc ccgcggccga
       61 caggacgtcg ccgccccggg tgatgaaatt cgccccgggc cgctcgagcc acagccggac
      121 ggccgtccga acgtcctcga cgatgacggc gtcaggcagg gcgggcaggc cgcccgggag
      181 acgtgatccc ggccggaggc gggatttgag cgttcccaga acacccggct gatcccgcca
      241 ttccccgccg tccggatcgc cggccgtccc tcccaagagg agataccgcc ctttcaaagt
      301 ccggccatcg agtccgcgaa gaagatcctc cggcgcgatg atcgcggccc tcgattcgtc
      361 tttccagaag acgtcgacta aggccgcgtc ggccgcttcc gtttcgacca gctcggccaa
      421 caagccgagg gcgccgtcca cggaggacgt ttcgtccgac gcgcgctcct tcacggcgat
      481 tttttcgagg gcctgaagat ggtggatgtc ggcgtcacgc agggcgcgca gctcttgcag
      541 ccggtgttct aggccagcga cggtctccgc ggtttcggcc agccggccgc ggagggcggt
      601 ccattccccg cggaccgctc cgctttcctc gagcgaggcc gcccgccggg attcgaggcc
      661 ccgcagatcc tcggatttgg cctcgaccag ggcccgggct tcctctttcc gggttttaag
      721 cttttcctcc tggcgggtaa gcatctcgag ctctttttcg accttagccg cgtcgttgcg
      781 ggccgccgtc cattcggcca gcttctgaag gtgatcggac cggagccggt cggactgctc
      841 caggagcggg gagatccgag ccgtcgtttc cgccagggtc cgttcgaagg agacgatcgc
      901 ctcctttttc acgacgaggg cggccgtcaa cgcctgtatc tcctcccggg accgggcgcc
      961 ttcttcctcg agcccgagca attccctgag ccgttcgtcg gcgtcggccg ccgcttttcg
     1021 ccggctctcc tcgatgaatt cgctccgctt ggcatcgcgc tcccgttcgg cctcgagccg
     1081 ggcgatctgg gatcgaatcg tataaagatt ctcccggcct ccggtgaacg cctgttccag
     1141 ctcccagaca ttcttgcgct tttggttgac ggccccctcc tcgtccgcca gccgggcctg
     1201 ttgttcgcgc tcgcggatgg aggattcttc atagcgggcc aggatgtccg cctggccggc
     1261 ggcaagctgg gcgcttttca accggaagtg gagaacgttc agctcccgga cgcgctcgcg
     1321 cagacgccgg taccgctcgg ccgctccggc ctgccgggcc agggaattct tggccttgga
     1381 gacttcggcg atgatgtctt ccaggcggac gagattccgc tcggagtctt cgagcttgtt
     1441 ttccgcctgg cgcttcttgt ccttgaaaaa ggccgttccc gcggcctcct cgatcagggc
     1501 ccgcttttcg accggcttgg aggtgacgaa cgtgccgatg gctccttgtt cgatcacgaa
     1561 atagcggttt tccgaaatgg tccgcttcca gagctcgtcc tggatgtcct tgagacggac
     1621 gattttcccg tcgagccggt attccgactc cccggtgcgg aacacgcggt ggttgacggt
     1681 gatctcatcc tcggagtttt ggagaaccag ggaaacgtcg gccatcccca tcggcggacg
     1741 cagggccgtg ccggcgaaaa cggcgtcgtc gattttctcg ccccggacgg tccggacgcg
     1801 ctggccgccc agaacccact ggatggcttc gacgatgttg cttttgcccg ttccgttggg
     1861 tccgatgatg atcgtgatgc ccggattgaa aaggattttc gtccggtcgg ggaaggtctt
     1921 gaatccttgg agctcgagtc tcttgatgta catgaatgat ctttcagggc agtattgtag
     1981 ccaaatcatc cccgattgaa aagcaaaaaa ccggcttgca atggaggggg gattatgtca
     2041 aagtgaaatc accatggaag acgacaagag gaaatatttc cggttcgagt gcctgattcc
     2101 ggtggatctt gtggagacgg gcgaccccga cgccaagccg gaggaagcgt tgatcggcaa
     2161 tgtctcccgg gaagggatgc gcctcgtgtt cgacctgggg cgcgcctttc aggaaggaga
     2221 caaggtccgg tttcagatcc agacgccgga agacaaccgg gtctgccgga tgaccggcga
     2281 ggtcatctgg tcccgctcgc agggggaaaa aatcgaggtc ggcttgaggc tccaggaaac
     2341 ggaaaaatgt tcgaaatcgg agctgttgga cctcggctac gaggcttggc ggaagcacca
     2401 gaaagagatc gagacaaaat agagggctca taaggaatcg gggcgggttc aggatcattt
     2461 ttttcctttt tcgccgtgct ccgggagcgg aatcttttcg gcgtcgcggg atttattttt
     2521 gggggcaagc ggcgctcgcc cggaaatccg cggcgagagc gcccggagcg gcgtttcggg
     2581 ggcgcggaag cctcgatttc ccgggccgaa gccgccgggc cggtcccggt cattgacaga
     2641 aacgcctgtt tcggctactt tatcactccc gccgcttagt gccagcgtag ctcagtcggt
     2701 agagcggctg attcgtaatc agcaggtcgg aggttcgatc cctctcgctg gctttcgatt
     2761 ttttcctttc ccgcgatcat ttttccttgc cttgtccctc tccttggggc atagtaaacg
     2821 aaaaggagga ggccatggct gattttgatt tttcctcccg gaagccggag atccatcccg
     2881 atacgttcat cgccgaaggg gcccgcctgg tcggccgggt ggtcctggcg gccgggtcga
     2941 gcgtctggta caactgcgtc ctgcgggccg acgtcggaga catcatcatc ggcgaaaaaa
     3001 ccaacatcca ggacaacagc acgattcatg tcgatttcga tctgccggcc gtgatcggcg
     3061 accatgtcac cgtcggccat ggggcgattc tccacgcttg caccatcgga tcccattgcc
     3121 tcatcggcat gggggccgtg atcctggaca aggcggtcat ccccccccgt tccatcgtcg
     3181 gggcgggggc cttgatccct ccccgaaaaa catacccgga aggcagcttg atcctcggct
     3241 cgcccgcgat cgtggcccgc aagctgaccg cagaagaaat cgaacgcctt cctgagcatg
     3301 cccgggaata tgtcttgctg tggaaggcga tcgccggccg gggaatcgga gggatttaaa
     3361 ccaggagtca attgttccgg ccgccttgca atttccgcaa ccgcgtgtta aggttgcgga
     3421 agggagggag ttcatgcaag ccatccttcc tcacaaaatc cggaaagcga gggaagccgc
     3481 ccacctcaac caaggggcat tcgcccgagc ggtgggactg tcgtccgaat acatctccct
     3541 cctcgaggcg ggcaagcgga ccccgtcgat ggccaccctc gaaaagatcg ccggtttcct
     3601 caaccgcgat ttgtcgtatt tcttccagga aaagccggct ccgccggatc ccttcgcctt
     3661 gctcttccgg gccgaggccg tcgatgagaa gacgcgggcc gagctccagc ggttccggcg
     3721 ctactgcgac gattacgtcc ggctggaaaa cctcaccggc cggcgcctag aaacggcccc
     3781 tttctactcc ggcctctcgc cggaacgcat ggccgatgag gagcggcggc gcctgggttt
     3841 aggggacgag cccatccggg acgtgttcgc cttgctcgag accaacggct gccgcctcta
     3901 ccggatgcct ttgcccgagg attccaaggt gagcggcgtc ttcctctact tggaacagaa
     3961 ggaagcggcc ttcgccttga tcaacagcgg cggttcgccc ggccggcagg tgttccacgc
     4021 cgcccatgaa tattgccact acctgaagga ccggttcgac gggccggtca tcgacggccc
     4081 cgacgtcttc atcgatgaat acgcctctct ctaccacccg cgggagccgt tcgcccaagc
     4141 cttcgccgcc cggttcctga tgccgccggg caagatcaag gagatcatcg agaaggattt
     4201 ccgtgcctcg cgcctggctt acgaccacgt gctgtacctc aagaggtatt tcggagtcgg
     4261 ggcctcggcc atgctccgaa ccctccggac catgagcctg gtctccgcgg cccaatacga
     4321 cgaattcgtc cggatcgatc cggggtcccg ggaaaaggac ctcttcggcc gggcgtccgg
     4381 cgaggacccc gttgcgggcg ggccggggac ggctcccgga ggggaaggag aagagcggat
     4441 cgaggccggg gcggcgtccg ggaccgccgg tccgccggcc cggatcaagc ggaaacccgt
     4501 ccccagcgac agataccgga tgctccaaaa agaagcgatc cggaaaatcg cccgggaccg
     4561 cgagctgcgg aaggccgttc agggaacgct tccgacgtaa gccgggcggg cttcaaggag
     4621 gtggcatggg cggaatcaaa tggtcctcgg ctgtcttgtt cctgtttgcg gcgacgctct
     4681 tgaccgccgc gttttcctgc tcctcctcga cgggggggaa ggggacgggc ggcgccgaat
     4741 gggtgatgat ggacgaatcc tacgttccga aagacgacgt cgaggcgttc atcaaggcgg
     4801 acgcggtcaa ccgcggcgct cttcccctgt tcatccggaa tcacaagtcc gacaagcggg
     4861 ccttgagcgg attcaagggg cggcaatacg ccgggccgag cccccggacc ctggaaatgc
     4921 tcaaccccgg gctggacgac tggatgctcg tggatattcg gtacaaatcg gaaagcgagc
     4981 gcgagatcgt ccggacggtc ctgtatcttt cgatccgggg cgtgtggaaa gtcggcgaca
     5041 gcgggacgct gatcgcctcg tcataattgg gagccacaac gccaatttac ctgtttagag
     5101 ggccggccga cgctcagcag atccggactt ccagcatccg gtcgatgcgg aaggtccgca
     5161 tgtcctcgcg ttcatggcaa taggcccgga cgccctcgaa ggctttgccc ccgtattcca
     5221 tctcctcgac ggcctccggc cggatccggc ggcggctttt cgtgtcatcc ggcttgaggt
     5281 agaggatctc gaggtcgcgg ccgtcccgga cggcctcctc gatgagccgt ttcttttcta
     5341 tgtagggctg gcgttcgtcc gcccggtcgt attgaaagcg ggttagaaac cgggaaaccc
     5401 gccagtcggt ccggatgtgc gagcggagga tgggcgtttt caggaccagt ccctcgaagc
     5461 tcgtgcagcg gctgagggcg acataaacct ggccgtgggc gaatgtcccc cggccgacgt
     5521 cgacgatgac gttccggaac gtcttgcctt ggctcttgtg gatggtgatc gcccaggcca
     5581 gcttgaccgg gtattgaatg aacgccccga cggcctcgct gacgacggcg tccttttccg
     5641 agtcgtacgc gaagcggtag aggtcccatt cgttgggggc gacttccacc cgcttgccgt
     5701 cctggagatc gacggcgatc cggtcgtccc ggcccttctc ccggaggact ccggcgaccc
     5761 ggccgatcga gccattgacc cagcggccgg ccgcgtcgtt gttcagcagc atcacttggg
     5821 ctccggcctt gatttcgagg tgttcgtcgg tcgggagcga ggagcgctcg aattccccct
     5881 cgacgatcgc ctcataggca tagagccggc cgggcaggag ggcgagcttt tcccggttgc
     5941 gggcggcggc caggtcgttg gtgctcgtca aggtcacata aaaatcgtcc tcgggcggga
     6001 cgaactccgg gtcgtaccgg gagtggagct tctccaggtc ttccggcccg gcggaccggt
     6061 tgcggacggc gttgagcagg gcgatgaagc cggcgtcggt ctggcgatag attttttcga
     6121 gctcgacgaa ttccaggcgg aaggaatccc gcagaagaca gtgggcggag aagaagtacg
     6181 gcgtttcgta atggagggtg aagagggttt tttccactcc ggcgacgacc ggcggcagct
     6241 ggtagagatc gccgatgagg atcaattgca ggccgccgaa cggccggcgg ggcttcggac
     6301 cgttcaggcg gaggaatttt tcgacgcaat cgagaagatc ggcccggacc atcgagattt
     6361 cgtcgatgac gacggcgtcg agctttcgga gcagggccgc ccgctcggga tcctttttcg
     6421 cgttgatttt gcgaaccttg accagggtga tgtccggctt gaagccgcag aacgaatgga
     6481 tggtttgccc gcggacgttc agggcggcga cgcccgtcgg ggcgaggaca gcgatcttct
     6541 tgagcgtcgt ttcccggaag tgctcgagaa gggtggattt tccggtcccg gctttcccgg
     6601 tgacgaaaac gggccgggcc gtctcctcca tccagtgaag ggcttgccgg aattggtcgt
     6661 tgatctcgat cgggccggag gcgtccatga gcctgaaagc ctcactgtat ccgaacccgt
     6721 ccttggattc aactcgcatt tcgtctttag ttccgtttcg ccgcggcgga gcggagggcg
     6781 atcgcccgga cggatcagaa ggaacaggcg cgcttgtttt tcaggttgta cttctggtga
     6841 tattcctcgg cccgggtgaa cggaccggcc ggaacgatct cggtgacgag gcggccggga
     6901 aggcggccgg accggtcgat ttccgccttg atttcctcag cctgtttccg ctgttcctcg
     6961 gtgtgataaa agaccgccga gcgatactgg cggcccacgt ccggcccctg gcggttgagc
     7021 tgggtcgggt catggaagga gaaaaacgcc tgaacaaggc cggcgtagga aattcgggcc
     7081 ggatcaaagg tcacccggac ggcctcggcg tggccggtcg tgtccgtgca aacctgttca
     7141 taagtgggat ttttcgtgcg gcctccggta tagccgacga ccgtttccag gacgcccggc
     7201 aggcggccga acttctcttc cacgccccag aagcagccgg cggcgaacgt ggccgtttcg
     7261 ggcccgcccg ccggggccgc ggcggacggc cggaaggcca gggcggcgga gttgacgcag
     7321 taatggcggc cggtgggggg agggccgtcg tcgaagacgt gaccgaggtg gccgccgcag
     7381 gcggcgcacc ggatttccgt gcggatcatt ccggccgatc ggtcctcgcg gcgctccagg
     7441 gcgccgggcg acgccgattc gaagaagctc ggccagccgg ttccgtgttc gtacttggtt
     7501 tcggagcgga agaggagggc gccgcaggcg gcgcaatgat agccgccgga ctcccagaag
     7561 tggtcgtaaa tcccgctgaa cggcggctcg gttccgcagg cgatcaacac ctggtactgg
     7621 gccggcgtca gcgctttctt ccattcctcg gggctctttt gaacgatttt cttcatcggc
     7681 gcgctttccc gggaatccgc cggaacggaa atatgaaccc gcggcgagga cgagacgaaa
     7741 tggacgccct tccaggccgt ccagccgagc agaagactga ggacggcgat cgcggccttt
     7801 cccttggggc tcatcctcga ctcccggttt aaaagatata aatcctacaa aaataatata
     7861 gaaagatttc agaaatcgtc aagggcccgg ccggccgcgg ccggcgaggg ggatcaggag
     7921 cgggtgagga cgctccggat cttgctttcg atttccgagg ccggggccag gcccacgatc
     7981 cgctcggcct cgcggccttc gcggaaaaca atcagcgtcg gaatggccat gatcccgtgg
     8041 gtccgggccg tcgattcgtt ctcgtccgtg ttgagcttgc gaaaatcgac ctccccgtcg
     8101 aactttccgg cgatctcctc gaggacgggg gccatcatcc ggcaggggcc gcaccagggg
     8161 gcccagaaat cgacgacgac ggggcggggg gctttgagca cctcggcctc gaaacgggct
     8221 tcgctcgttt cagggacgtt cttcatcaag attctcctta ggggaaaagt cgcgcaaaat
     8281 gcgttcgatg cggggatcgg tcacctcgta gcagattttc gccccgcgac gccggccggc
     8341 gataagaccg gcggttttca agatcttcaa gtgctgggac acgttgggtt ggctgatc
//
LOCUS       DAOOXH010000099         8369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_9446_length_8369_cov_7.102718, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000099 DAOOXH010000000
VERSION     DAOOXH010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 8369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8369
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9446_length_8369_cov_7.102718"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PKV64_09840"
     CDS             <1..703
                     /locus_tag="PKV64_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPN17412.1"
                     /translation="EIDKISRKSENPSITRDVSGEGVQQALLKILEGTKASIPPQGGR
                     KHPHQEFIPVDTTDILFICGGAFVGLEKIIAQRLGRTIVGFGGEARSRRVEDAALLED
                     LIPQDLIKYGLIPELVGRIPVVSAFSELDLEELAAILTRPKNALIKQYQKLFEMEGVD
                     LEFTDEALAAIAQKSVARKLGARGLRSIIEDLMLDLMFDLPTSKKSRRIKITKRMVEE
                     NELAFDRPRKPSPGA"
     gene            706..3105
                     /gene="lon"
                     /locus_tag="PKV64_09845"
     CDS             706..3105
                     /gene="lon"
                     /locus_tag="PKV64_09845"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPN17413.1"
                     /translation="MDPVNDALEVLKTVPLIPLRDLVVFPSTLVPFIIGRPSSIQALE
                     KADESEKTVFLAAQMDPSLDNPEPKDIHSFGITARVIRTVPMDDKNVKVIVEGRRRAR
                     ILEYVSSYPFYQVLVKETPPSEETAASLADVLKRVLLMFEEYLKLNQNANFQTIIPAL
                     RDHSPERIADIISSHLFIPLEEKQSLLETIKAGERLSHLEFLLEHEIKRIQAGLRRKS
                     GKSGESGRRAEGTTTVFPAGGKKDDQANEMDELKKKVLAAQMPPDALEKARKEIERLE
                     AMPPMSAEATVCRNYLDWLIALPWVKRSREKRNIKEAERILDEDHYGLEKVKERVLEY
                     LSVRQLVKTPRGVILGLIGPPGVGKSSLAKSIARATGRKFVRLSLGGVRDEAEIRGHR
                     RTYIGAYPGRIIQMLKRAGTKNPVFLLDEVDKMSMDFRGDPASALMEVLDPEQNNAFL
                     DHYIDTDFDLSQVMFIVTANMLEPIPRPLIDRMEIIRIAGYTEPEKLQIARRFLWPKQ
                     LTAHGLTEKNLTISDAGLAKLINEYTREAGVRNLERELTSICRKAVKRVVLHGASHRE
                     RVSAKNLESYLGVPKFRRKALDREDETGVSIGMAWTEFGGELLTFEATKVHGKGNFVL
                     TGQLGEVMQESARTAFSYVRAKIFELNIAKDVTKDFDIHIHVPEGATPKEGPSAGITI
                     AVAILSLMTEIPVCKKIALSGEITLRGRVLPVGGIKEKLLAAHREGIREAILPRDNKA
                     DLKDLPKNVAQDMTIHLVETMDEVLKIVLTREMPSVQTPPEGASDSSTPAGEGEAVRM
                     H"
     gene            3249..4517
                     /gene="rho"
                     /locus_tag="PKV64_09850"
     CDS             3249..4517
                     /gene="rho"
                     /locus_tag="PKV64_09850"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPN17414.1"
                     /translation="MELEEQETKVILKIAAEAGLPAEEIEHEKDSRHDLIFKILEAQA
                     KKQGLLFSEGVLETLEDGFGFLRAPEFSYLPSQDDIYVSPSQIRKFQLRTGDTISGVV
                     RPPKNGEKYFALIKVEAVNFMHPDVIIEQRRNVHFEQLTPLYPFEMIKLQDGNPKNLN
                     ARIMEMMTPIGKGQRGLIVSPPRAGKTTLLKTIAKSIEKNHPEIYLIVLLVAERPEEV
                     TDFQRSVAGEVVASTFDEPPARNAQVANIVLEKAKRLVEIKRDVVILLDSVTRLARAH
                     NAIIPPSGKVLSGGIDANALNKPKKFFGSARKVEEGGSLTIIATALIDTGSRMDEVIF
                     EEFKGTGNMEINLDRRLVDKRTFPAIDMNRSATRKEELLIEESELNRIWILRKVLSQL
                     SEVEAMELLIDKLGKTKTNADFLNQMSKGL"
     gene            complement(4750..>5491)
                     /locus_tag="PKV64_09855"
     CDS             complement(4750..>5491)
                     /locus_tag="PKV64_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17415.1"
                     /translation="AAQPSPGGENDLALAAALPILRLRRASGLNRLEAFLLLFDQEKN
                     LAGLNRLELDAAKTSDEAVFCGSFSGVPAGRFECRIVVRNIETGDGAVATSAVDIPRR
                     GEPGFKLFPPLLLAPLEKPRPVFGALPDFHGFDSARFAPLTGEIPQGTKILSVLLPCS
                     FHQYEGGDIVFRAQAVDLASGGRTWLPLSASAYRQIDAVSYLVDLETGGLPAGTYTLY
                     FFAEDPGKRYPSSFVRAPLIIEPADPEP"
     assembly_gap    5492..5501
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5502..6871)
                     /locus_tag="PKV64_09860"
     CDS             complement(<5502..6871)
                     /locus_tag="PKV64_09860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPN17416.1"
                     /translation="MNPKTLFSFHLAFLIGFFRLVAVGAAQVEQPPLKHDVAVTMKLI
                     QVYVSDKNGRPVRDLDSPEFEVISNGRPQRIVAFERHVLPAEEAGETEAVEIESLIMS
                     RKFFFLFDFAFNDVGGIAMSKKTALDFLDTHALPADEIGILSYSVEKGLTLHEYLTSD
                     RGKIREIVRDLGTGVGLGRAGTFWDELTAEKTAKLDRGASAALAAAARQEFKRAAQAQ
                     YQMEILKFSSSLKDFAAGLRYMPGIKHVVLFSKGPPDFLMYQRADQIAPEANRMNLNA
                     SDGLNLRLRYESMIRELASSNSPVIAVNVEGLASRVRDMDFEELSRSARPSSQSSSGT
                     ALNLVERNMVGDSALIEMARLSGGKYFGGMNDVQKISREIQDLTGTFYVLGFIVPEKW
                     DGKFEKIEVKVKRRGCEVHSQKGYYNRKPFAKLGALEKHLHLVDLALSEKPLFSDPLR
                     FSMSAAA"
     gene            6984..7883
                     /locus_tag="PKV64_09865"
     CDS             6984..7883
                     /locus_tag="PKV64_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPN17417.1"
                     /translation="MNIILKTALGLMTTLFGFIYFRDAIKKRALFSRKPWAGFFGTGL
                     ITNFFDTLGIGSFAQQTAIFKFFRLVDDRIIPGTMNVGNTIPTVLQAFIFMTVVKVDA
                     VTLVAMSMAAPAGSVIGAGIVAKMERGKIQLGMGIGLLVIALIILAGQLNVMPVGGEA
                     FGLTGWKLSLAVALSFVFGALQTIGIGFYAPCMAIVYALGMHPLTAFPIMMTATAMLM
                     AAGSARFVTADAYDRKAAVGVTAGGVIGVFIAAYVVKSLPLTVLKWVVCAVVLYTAAW
                     MFLSAFKSWKERAVVPILDSSKY"
BASE COUNT         1806 a         2457 c         2500 g         1596 t
ORIGIN      
        1 cgagatcgac aagatatcgc gcaagtcgga gaacccgtcc atcacccggg acgtttcggg
       61 ggagggcgtt cagcaagccc tcctgaagat tctcgagggg acgaaagcca gcatccctcc
      121 ccagggcggc cgcaagcatc cccaccagga attcatcccc gtcgatacga ccgacatcct
      181 tttcatctgc ggcggggcgt ttgtcgggct cgaaaagatc atcgcccagc ggttgggccg
      241 gacgatcgtc ggcttcggcg gcgaagcccg ctcccggcgg gtcgaggacg cggccttgct
      301 ggaggatttg atcccccagg atctcatcaa gtatgggctg attcccgagc tcgtcggccg
      361 cattccggtc gtttcggcgt tttccgagct ggatttggaa gagttggcgg ccatcctgac
      421 ccgcccgaaa aacgccttga tcaagcaata ccagaagctg ttcgaaatgg agggcgtcga
      481 cctggagttc accgacgagg ccctcgccgc gatcgcccaa aaatcggtgg cccgcaagct
      541 cggagcccgc ggcctccggt cgatcatcga ggacttgatg ctcgacctga tgttcgacct
      601 gccgacatcc aaaaagagcc gccgcatcaa aatcacgaag aggatggtcg aggaaaacga
      661 gttggccttc gaccggccgc ggaaaccatc ccctggagca tagtcatgga tcccgtcaac
      721 gacgccttgg aagttttgaa aaccgttccg ctgatcccgc ttcgggactt ggtcgttttc
      781 ccctcgactc tcgtcccgtt catcatcggc cgcccatcgt ccatccaagc cctggaaaag
      841 gccgacgaat ctgaaaaaac cgtctttctg gccgcccaga tggatccctc tctcgataac
      901 cctgagccca aggatatcca ctcgttcgga atcaccgccc gcgtcatccg gacggtgccc
      961 atggacgaca agaacgtcaa ggtcatcgtc gaggggcgcc gccgggcccg gattcttgaa
     1021 tatgtctcct cctatccttt ctaccaggtc ctggtcaagg aaacgccgcc ttcggaggag
     1081 accgcggcct cgctggccga cgtcctcaag cgcgttctgc ttatgttcga ggagtatctt
     1141 aagctcaacc agaacgcgaa ttttcaaacc atcatccctg ctctccggga tcattccccc
     1201 gagcggatcg ccgacatcat ctcctcccac ttgttcattc ccctggagga aaaacagagt
     1261 cttctggaga cgatcaaggc cggagaaaga ctttctcacc tggagtttct tctggagcac
     1321 gaaatcaagc ggatccaggc cggcctccgg cggaaatccg gcaagtccgg ggaatccggc
     1381 cggcgggcgg aggggacgac gacggttttc ccggccggcg ggaagaaaga cgaccaggcc
     1441 aatgagatgg acgagttgaa gaaaaaggtc ctggccgccc agatgcctcc cgacgccttg
     1501 gaaaaagccc gcaaagagat cgagcgtctc gaggcgatgc cgccgatgtc ggccgaggcc
     1561 acggtttgcc gcaattacct cgactggctt atcgccttgc cctgggttaa gcggtccagg
     1621 gaaaagcgta atatcaagga agccgagcgc atcctcgatg aagaccacta tgggctggaa
     1681 aaggtcaagg agcgcgtcct ggaatacctg tcggtccgcc agctcgtcaa aacgccgcgg
     1741 ggagtcatcc tcggcttgat cggccctccc ggcgtgggga agagctccct ggccaagtcc
     1801 atcgcccggg cgaccggccg gaaattcgtc cggctttcgc tggggggcgt ccgtgacgaa
     1861 gccgagatcc ggggccatcg ccggacgtac atcggggctt atcccggccg gatcatccag
     1921 atgttgaaac gggccgggac gaagaacccg gtctttctcc tggacgaagt cgacaagatg
     1981 agcatggact tccggggcga tccggcctcg gccttgatgg aagtcctcga tcccgagcag
     2041 aacaacgcct ttctcgatca ttacatcgac acggatttcg acctttccca ggtcatgttc
     2101 atcgtcacgg ccaatatgct cgagcccatc ccgcggcccc tcatcgaccg gatggagatc
     2161 atccggatcg cgggatacac ggagccggaa aagctccaga tcgcccgccg gttcctctgg
     2221 cccaagcagt tgacggccca cggactgacc gaaaagaatt taacgatttc agacgccggc
     2281 ctggccaagc tgatcaatga gtacacccgc gaggcggggg tgcgtaacct cgagcgggag
     2341 ctgacctcga tctgccgcaa agccgtcaag agggtcgttc tccacggggc ttcccaccgg
     2401 gaacgggtct cggccaagaa ccttgaatca tacctcggcg ttcccaagtt ccggcggaaa
     2461 gccctggacc gggaggacga aaccggggtg tcgatcggaa tggcctggac ggagttcggc
     2521 ggcgagcttc tgacgttcga ggccaccaag gtccacggca aaggcaactt cgtcctgacc
     2581 ggtcagttgg gcgaggtcat gcaggagtcg gcccggaccg ccttcagcta cgtccgggcg
     2641 aagatcttcg agctgaacat cgccaaggac gtgaccaagg atttcgacat ccacatccac
     2701 gtcccggagg gggcgacgcc gaaggaaggt ccctcggccg ggatcaccat cgccgtggcc
     2761 atcctgtccc tgatgacgga gatcccggtc tgcaagaaaa tcgccctgag cggcgagatc
     2821 acgcttcgag gccgggtgct tcccgtcggc gggatcaagg agaagctcct ggccgcccac
     2881 cgcgaaggca tccgggaagc gatcctgccg cgggacaaca aggccgacct caaggacctg
     2941 cccaagaacg tcgcccagga tatgacaatc catcttgtcg aaacgatgga tgaggtgttg
     3001 aaaatcgtct tgacccggga aatgccctcg gtccagacgc cgcccgaagg ggcttcggat
     3061 tcctcgactc ccgccggaga aggggaagcc gtccggatgc attaagaatt caatcgcgag
     3121 gtgcagagat gtcgacaaga gaaccgagaa acaaccggga acgagagccc cgggagcagc
     3181 gtgaaccgcg ggaaacgaga gagccgcgcg agtacccggc ggaccgggaa acccgcgaat
     3241 ggggcctcat cgagctcgag gaacaggaga cgaaagtcat tttgaaaatc gcggccgagg
     3301 cgggcttgcc ggccgaggaa atcgaacacg aaaaagacag ccgccacgac ctgatcttca
     3361 agattctcga agcccaggcc aaaaagcagg gcctgctctt ttccgagggc gtcctggaga
     3421 cgctcgagga cgggttcggg ttccttcggg cgcctgaatt cagctacctg ccgagccagg
     3481 atgacatcta cgtctcgcct tcgcagattc ggaaattcca gctccgcacc ggagacacga
     3541 tctccggcgt cgtccggccg cccaagaacg gagagaagta cttcgccttg atcaaggtcg
     3601 aagccgtgaa cttcatgcac cccgatgtca tcatcgagca gcgccgcaac gtccattttg
     3661 aacagctgac gccgctctat cccttcgaga tgatcaagct gcaggacggc aatcccaaga
     3721 acctgaacgc ccggatcatg gaaatgatga ccccgatcgg caaggggcag cgcgggctga
     3781 tcgtctcgcc tccccgggcc ggaaaaacga ctcttctcaa gacgatcgcc aagtcgatcg
     3841 agaaaaacca ccccgagatc tacctcatcg tcctgctcgt cgccgaacgc cccgaggaag
     3901 tcacggactt ccagcggagc gtggccggcg aggtggtggc ctcgacattc gacgagccgc
     3961 cggcccgcaa cgcccaggtg gccaacatcg tcctggagaa ggccaagcgc ctggtcgaga
     4021 tcaagcggga cgtcgtcatc cttctcgatt cggtcacccg gctggcccgg gctcacaacg
     4081 cgatcattcc gccctcgggc aaggtcctgt cgggcggcat cgacgccaat gccctgaaca
     4141 aacccaagaa attcttcggc tcggcccgga aggtcgagga ggggggaagc ctgacgatca
     4201 tcgccaccgc cctgatcgac accggaagcc ggatggacga agtcattttc gaggagttca
     4261 agggcaccgg caacatggaa atcaacctcg atcggcgcct ggtcgacaag cggacgttcc
     4321 cggccatcga catgaatcgc tcggccaccc gcaaggaaga gttgctcatc gaagagagcg
     4381 agctcaaccg catctggatt ctccgcaagg tcttgagcca gctgtccgag gtcgaggcga
     4441 tggagctttt gatcgacaag cttgggaaga ccaagaccaa cgcggacttc ctcaaccaga
     4501 tgagcaaagg gctctaagcc cggccggcgc ggggcggggg agagggcgat tccactgggc
     4561 ggccggacac ggcgccggct tcaagaattg actctcccgc ctgaggctct ccgggcttat
     4621 ttccccgtga gcctcaatcg aaaacccgct cttgctttgt cgtcgccgac cacccgggcc
     4681 ttgtcgagcg tcgccagcat gtccctcgcg aacgatcgcc cttgacggtg atcggctcga
     4741 gatcgtcgat caaggctccg ggtcggctgg ctcaatgatg agcggggcgc ggacgaacga
     4801 agagggatag cgcttgccgg gatcctcggc gaagaaatac agcgtgtagg tccccgccgg
     4861 caacccgcct gtctcgagat cgaccaggta agagaccgcg tcgatctgcc gataagcgga
     4921 ggccgacaag ggaagccatg tcctcccgcc ggaggccagg tccaccgcct gcgcccggaa
     4981 gacgatgtcc ccgccttcgt attgatggaa ggaacagggg aggagaaccg aaaggatttt
     5041 cgtgccttgg ggaatttcgc cggtcaacgg ggcgaagcgg gcggagtcga atccatggaa
     5101 atcgggaagg gcgccgaaaa ccggcctcgg cttttccagc ggggcgagca agagcggagg
     5161 aaagagcttg aatcccggtt cgccgcgccg gggaatgtcc accgcggacg tcgcgaccgc
     5221 gccgtctcct gtttcaatgt ttcggacgac gatccggcat tcgaaccggc cggccggaac
     5281 gccggaaaaa gaaccgcaaa aaacggcttc gtcggatgtc ttggccgcgt ccagttccag
     5341 gcggttcagc ccggccaggt tcttctcttg gtcgaacaga agcagaaaag cctcgaggcg
     5401 attcaggccg gaggcccggc gcagcctgag aatgggaagg gcggccgcaa gggccaagtc
     5461 gttttccccc ccggggctcg gctgggcggc gnnnnnnnnn ngcggcggcc gacatggaga
     5521 accgaagggg atccgaaaag aggggttttt cgctcagggc caggtcgacg aggtggagat
     5581 gcttttccaa ggcaccgagc ttggcgaacg gcttgcggtt gtaataccct ttttgggaat
     5641 ggacttcgca gccgcgtcgc ttgaccttga cttcgatttt ttcaaatttg ccgtcccatt
     5701 tctccggaac gatgaatccg agaacgtaaa acgttcccgt cagatcctgg atttcccgtg
     5761 agattttttg aacgtcgttc atcccgccga agtatttgcc tccggaaaga cgggccattt
     5821 cgatcagggc gctgtccccg accatgttcc gctcgacgag attcagggcc gtgccggagg
     5881 aggactggga ggacggacgg gccgaccggg acaattcctc gaaatccatg tcccggacgc
     5941 gggaagccaa tccttccacg ttcacggcga tcaccgggct gttggacgag gcgagctccc
     6001 ggatcatgga ttcatagcgc aggcgcaggt tcaaaccgtc cgaggcgttg agattcatcc
     6061 ggttggcttc gggcgcgatc tggtcggccc gttgatacat caggaagtcc ggggggcctt
     6121 ttgagaacag gacgacatgc ttgatgccgg gcatgtagcg gaggccggcc gcgaaatcct
     6181 tcagggatga ggaaaacttc aggatttcca tttggtattg ggcttgggcg gcccgcttga
     6241 attcctggcg ggcggccgcg gcaagggcgg ccgacgcgcc ccggtccagc ttcgccgttt
     6301 tctctgcggt cagttcgtcc cagaacgttc cggccctgcc gaggccgacg ccggtcccca
     6361 gatcgcggac gatctcgcgg atttttccgc gatccgaggt caagtattcg tggagggtca
     6421 ggcctttctc cacggaatag gaaaggattc caatctcgtc ggccggcagg gcatgggtgt
     6481 cgagaaaatc gagggcggtc ttcttggaca tggcgatgcc gccgacgtcg ttgaaggcga
     6541 aatcgaaaag gaagaaaaat ttccgggaca ttatcaggga ttcgatctca acggcttccg
     6601 tttctccggc ctcctcggcg ggaagaacat gccgctcgaa ggcgacgatc cgttgcggcc
     6661 ggccgttcga gatcacttcg aattccgggg aatcgagatc ccggacgggc cggccgtttt
     6721 tatccgagac gtaaacctgg ataagcttca tggtgacggc gacgtcatgc ttcagcgggg
     6781 gctgttcgac ttgggccgcc cccacggcga cgagccggaa aaagccgatc aagaaagcaa
     6841 gatgaaaaga gaatagagtt ttcggattca cggcgcgcac acctccgctt ttttcccccg
     6901 acaactctcg cgatcagcgt aacataaaac tgaaatgaaa tatacttgac gcggatcagg
     6961 caatcccata ggatgaaaag cccatgaaca tcatcctgaa aacagccctc ggcttaatga
     7021 cgacgctctt cggttttatt tattttcgag acgcgatcaa aaagcgggct ctcttttccc
     7081 gcaagccgtg ggccgggttt ttcgggacgg ggcttatcac gaactttttc gacactctcg
     7141 gcatcggaag cttcgcccag cagacggcga tctttaagtt tttccgcctc gtcgacgacc
     7201 ggatcattcc cgggacgatg aacgtcggca acacgatacc gacggtcctc caggcgttca
     7261 tttttatgac ggtcgtgaaa gtcgacgcgg tgaccctggt ggcgatgtct atggccgccc
     7321 cggccggatc cgtgatcggc gccggcatcg tggccaagat ggagcgcggc aagatccagc
     7381 tcggcatggg catcggcctt ctggtcatcg ccttgattat attagcgggc cagttgaacg
     7441 tgatgccggt cgggggagag gccttcggct tgacgggctg gaagttgagc ctggcggtcg
     7501 ctttaagttt tgttttcggg gccctccaaa cgatcggcat cggattctat gctccgtgca
     7561 tggccatcgt ttacgccctg ggcatgcatc ccttgaccgc gtttccgatc atgatgacgg
     7621 ccacggccat gctcatggcc gccggcagcg cccgtttcgt cacggctgat gcctatgacc
     7681 ggaaagccgc ggtcggcgtc acggctggcg gcgttatcgg cgtgtttatc gccgcttatg
     7741 tcgtcaagtc gctgcccttg accgttctca aatgggtcgt ctgcgccgtc gttctttaca
     7801 cggccgcttg gatgtttctc agcgctttca agtcgtggaa agaacgggcg gtcgtgccga
     7861 ttttggattc ttccaaatat taaaacgcgg ctttggctcg cgacgaaatt aattcattcg
     7921 gccgaatgtg atatatcgtt attttatcaa ttaattgttc ctggtgttta aggagaaaag
     7981 ctccgaaacg ctcgatttga ttcacccgaa aaaaatccgc ggccgtggtt ttttgaaaag
     8041 aacttatgcc ggctgttatc gtctagcttt caaacatttc ttcaccgtcg cctttggaag
     8101 ttgattgaaa cagggccgaa gactcatcca aaaagacgga ttctttcgat cgacgccgat
     8161 gattagacaa agggcctgaa tcatgaaaac ggggcaatca ttgaataacc ggaaaagggg
     8221 aaaatatcca caggtgccca atttacgggc atcaaggata ttcacagcct tatgaaatct
     8281 ttaagtgaat gataataaat aagataagag ttcgcctggt attgggtcgc ataacatata
     8341 ttatttatta tcattcactt aaagatttc
//
LOCUS       DAOOXH010000100         8004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_10078_length_8004_cov_7.140269, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000100 DAOOXH010000000
VERSION     DAOOXH010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10078_length_8004_cov_7.140269"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PKV64_09870"
     CDS             <1..296
                     /locus_tag="PKV64_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17418.1"
                     /translation="ASDCGSEGWGFESPHPPHFFQRPVLKRMPNGLFRSFPEFTPFDP
                     QIDDEISAIPRLPSGRYNILIHNVFHQSAFGSRMNAMVKKVNDHPPSRFHVPD"
     gene            426..728
                     /locus_tag="PKV64_09875"
     CDS             426..728
                     /locus_tag="PKV64_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPN17419.1"
                     /translation="MPDHPLSPMKKLDPEFMKHLEETEALVYADGALPRKIKLLMAMA
                     FDAAAGAAGGVRALAEQAMRAGATKEEIAETLRVAFLLSGVGSLYAASFGLKDILD"
     gene            complement(810..1934)
                     /gene="amrS"
                     /locus_tag="PKV64_09880"
     CDS             complement(810..1934)
                     /gene="amrS"
                     /locus_tag="PKV64_09880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPN17420.1"
                     /translation="MKKRDFLKCILYAGGACVCGWTPAADGAPAGGAAAKSFVKEREA
                     GHYSPTPRGVKCLICPNECTLKDGELSDCRNRIARGGKLYTMAYGNPCSVNVDPVEKK
                     PLLHFLPESRTFSIATAGCNFACLNCQNWEISQVSPTETRNQDAPPEAVVDAAERMGC
                     RSIAYTYSEPITFFEYMRDTAARARSRGLKNIMVSNGSINTGPLLELCQVIDAANIDL
                     KSFDPNIHLRLTGGKVEPVLATLKTMKEQGVWLEITNLVVPGWTDDFGMIADMCGWLA
                     KEGFADVPLHFSRFQPLYKLTRLPPTPVATLTKAKEIAVRAGLKYVYIGNVPGIGGED
                     TCCPSCKTLLIKRLGFTIEENKLSGGSCSVCGTAIPGRWS"
     gene            complement(1931..4438)
                     /locus_tag="PKV64_09885"
     CDS             complement(1931..4438)
                     /locus_tag="PKV64_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17421.1"
                     /translation="MNAPYGLIPLGLFLALLYGISFLFSRLGILSTPAHRKIWNYALL
                     AVFLVTAGLGVLMAVQVNYRLEIPWAEKALKIHVNFGLGMSLIGLFHLCWHAPYFFKR
                     RKTRPVPIAASISAGPSPTAARARRDLALPFAVGFSGVAVQTLLIREFLTLFEGNELT
                     LSLIIFLWLLLSGAGSLTGARAQVPDAASSREAVKRASTLVKALLVVPLAVFPLMFPL
                     KSLLFAPGIEAGPPAMSGFLALLLLPYCFLNGFSFTWTARVLRREGRSLRTVYGWESV
                     GGAAGGVFCAAAVLPGLPSLAIVFAVSSGLFFLLAAAGRSENRAWWVFPAVLLAAAVL
                     FITAGGDAWMIQRFHPNERLVGTVSGGAGRLTVTRSGDQVNVYENGVLVHASGNAMLN
                     EELAAFALIQTEHPDRVLLIGGLLSGLPAELAKYGAGQVDIAEPDPHLFRLAGKLGLA
                     PSASGVLPIRGTPFRWIETAGVRYDAVLIALPGPWNLQLNRYYTAEFFARVKSVLAPG
                     GVAAAVLPGTANYVSESAAASLGSVAAAARKCFAETAVFPGEMSVLVMSGRPLTTDIL
                     SGLARRGIANLYVRPGYFDEELFKTRIEQVNAVIREAAPNADLKPAAYLSHIRWWLGR
                     FPEKILGPAAVLLALLVLWGILAGRSSLAGMFIMGAASSGSSILLLLLIQIAVGALYL
                     WTGLFLGIFMVGLAAGSLSGPRPAEKAGRAKTGGPLAVFAAVSASAGLLAPQLAHGRG
                     PGFLKIVLLLSAGFVLAAAVGRFFAGLSTALEKNSARHDRLYGYDLLGSALGAIAFPM
                     VVIPLAGVRNALFALALAGLLAWAVVVLGGRRRMEKK"
     gene            complement(4445..5950)
                     /gene="amrB"
                     /locus_tag="PKV64_09890"
     CDS             complement(4445..5950)
                     /gene="amrB"
                     /locus_tag="PKV64_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPN17422.1"
                     /translation="MRLEIVPALLGFVFVLVFPAFGRMNAPLDDTDRKPAVAGSFYPA
                     DPAALRNMVSAFLENGQKGPLPGTVRALVVPHAGYVFSGSVAASGYNQVQADYPYARV
                     FILASSHRLDLGKASVFTEGDYLTPLGRVEVDREVTAVLSGTRSCFTGDPEAHREEHS
                     VEVQLPFLQVRLNKPFKIVPIVLATQTPSVCRKIAEALKPYFTKDNLFVVSADFSHYP
                     SADDARKADAATAEGFCANDPDRFLDVLRSNARLGLPGLVTSMCAWPAALTLLYLTEG
                     VADLEFTRVDSKNSGDVEPYGDKSRVVGYEAIAVSEREEKKFELSAEDREVLLRIARA
                     SVEAAAKAEPLPGLTPGQYAPILREPAGAFVTLRKNGDLRGCLGTFEPRKPLFQVVQE
                     IAAASAVRDIRFPPVRPEELDKIRIEISVLTPRRKIKNIKEIELGKHGLYIEKGNQHG
                     TFLPQVAAETGWTLREFLGHCAQDKAGIGWNGWKQADIYVYEALIFEESGR"
     gene            complement(6214..6522)
                     /locus_tag="PKV64_09895"
     CDS             complement(6214..6522)
                     /locus_tag="PKV64_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17423.1"
                     /translation="MKKATAAVVLATLAVFCAWSCAPGPNTAEKTPDTEGKVAGFWLG
                     LWHGLISPVTFIISIFSKTVRLYEVHNNGGWYNFGFVIGAGLFLGGGIFGHKKRRSDK
                     "
     gene            6723..7166
                     /locus_tag="PKV64_09900"
     CDS             6723..7166
                     /locus_tag="PKV64_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17424.1"
                     /translation="MKTPSREAAAGERRERLFWGAFHILILAFAVWLVSPFIVISSSA
                     FSSRQGYGVRLALGLLIFLMYVGKWSFDVLAPQGLARRVSRLKTAALIVYIVIVISFM
                     IYIVAQAASLYLRTAVDESADNPDQVITRLERVENPAFGPSAFFP"
     gene            7306..>8004
                     /locus_tag="PKV64_09905"
     CDS             7306..>8004
                     /locus_tag="PKV64_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic hydrocarbon degradation protein"
                     /protein_id="HPN17425.1"
                     /translation="MRKITLLLALGLLVLGTAQAAIITNTNQSTAYIRMLSRNATTEI
                     DAVYYNPAGLVRLADGWHFSLSNETISQTKTVVNDFPLLNNGTYEGKVSVPIYPDAYI
                     AWKKGKIAVSVGFGPNAGGGSADYQTGLPSFETGISMLPTLITMMGLPTTKYSADIAF
                     KGSSVFLGFQGNLSYALSETFSLAAGFRYISATNTYEGRLKSVMINPQHPLLNPSGGM
                     MSAVTFFTAIGQPGL"
BASE COUNT         1553 a         2517 c         2502 g         1432 t
ORIGIN      
        1 gagcatcgga ctgtggatcc gaaggttggg ggttcgagtc tcctcaccca ccccattttt
       61 ttcaaagacc tgttttgaag agaatgccaa acggtttgtt ccgctcattt ccggaattca
      121 cccccttcga tccgcagatc gacgacgaga tttccgcgat tccacgtttg ccctccggcc
      181 gctataacat attgattcat aacgtttttc atcaatcggc cttcggttcc cgcatgaatg
      241 ccatggttaa aaaagtgaat gaccatccgc cttcccgttt tcatgtgccg gactaagctg
      301 ccaatattcc tcatgagacc gaatccgagg gctttcttaa aggatagatt cgcccttctt
      361 attttcaaaa attgaaaatg gcggcattga taagataatg acctaaaacc acaaggaggt
      421 ttctcatgcc tgatcatccg ctgtccccca tgaagaaact tgatcctgaa ttcatgaaac
      481 atctggagga gaccgaggcc ctcgtctatg ccgacggcgc cctgccgcgc aagatcaagc
      541 tcctgatggc gatggccttc gacgcggccg cgggagcggc cggcggtgtc cgggccctgg
      601 ccgagcaggc gatgagggcc ggggcgacga aggaagaaat cgccgaaacc ctgcgcgtcg
      661 ccttcctcct gtcgggagtc ggcagtctct acgcggcctc tttcgggctc aaagacatcc
      721 tggattgatt ggagaaggcg gcgggacgag cgcctctccc cggttcccgg ctttcccggg
      781 atttcttgac agacaaacgg gccgggatgt cagctccagc gcccgggaat cgccgttccg
      841 cagacggagc atgacccgcc ggacagcttg ttttcctcga tcgtgaaacc cagccgcttg
      901 atgaggaggg ttttgcagga cgggcagcag gtgtcctcgc ccccgatgcc ggggacgttc
      961 ccgatgtaga cgtatttcaa cccggcccgg acggcgatct ccttggcctt cgtcagggtc
     1021 gccaccggcg tgggcggcag ccgggtcagc ttgtagagcg gctggaaccg gctgaagtgg
     1081 agggggacat cggcgaagcc ctcctttgcc agccagccgc acatatcggc gatcatcccg
     1141 aaatcgtccg tccagccggg gacgacgaga ttggtgattt ccagccagac gccctgttcc
     1201 ttcatcgtct tgagcgtcgc cagcaccggc tcgactttcc cgccggtgag cctcaggtgg
     1261 atgttgggat cgaaggactt gaggtcgatg ttggccgcgt cgatgacttg gcacagctcg
     1321 agcagcggcc cggtattgat ggacccgttg gagaccatga tgttcttgag gccccggctc
     1381 ctggcccgag ccgccgtgtc ccgcatgtac tcgaaaaacg tgatcggctc ggagtacgtg
     1441 taggcgatcg accggcaccc catccgctcg gcggcgtcga ccacggcctc ggggggcgcg
     1501 tcctggttgc gggtttcggt cgggctgacc tggctgattt cccagttctg gcaattcaag
     1561 caggcgaaat tgcagccggc cgtggcgatg ctgaacgtcc gcgattccgg caggaaatgc
     1621 agcaagggct ttttttcgac ggggtcgacg ttcaccgagc agggattccc gtaggccatc
     1681 gtgtacagct ttccgccccg ggcgatgcgg ttccggcaat cgctgagctc gccgtccttc
     1741 agggtgcact cgttcggaca gatgaggcat ttgacgcccc gcggcgtcgg gctgtaatgg
     1801 ccggcctccc gctccttgac gaagctcttg gcggccgctc ccccggccgg ggcgccgtcc
     1861 gcggccggcg tccatccgca gacgcaggcc ccgccggcgt aaagaatgca tttcaagaaa
     1921 tcgcgttttt tcattttttc tccatccttc gcctcccgcc caagacgacg accgcccagg
     1981 ccaagagacc ggccagggcc agggcaaaca gggcgttgcg gacgccggcc agcggaatga
     2041 cgaccatcgg gaaagcgatc gctccaaggg cggagccgag caagtcatat ccgtacaggc
     2101 ggtcgtgccg ggcggagttc ttttcgaggg cggtcgaaag gccggcgaaa aaccgtccga
     2161 cggccgccgc caggacgaag cccgccgaaa gcaagagcac gattttcaga aatcccggtc
     2221 cccggccatg ggccagctgg ggcgcaagca gcccggccga ggccgagacg gcggcgaaga
     2281 cggccagagg tcccccggtc ttcgccctcc cggctttctc cgccggccgc ggaccggaca
     2341 ggcttcccgc ggccagcccg accatgaaga tccccaggaa aagcccggtc cagagataga
     2401 gcgcgccgac ggcgatctgg atgagcagca gcaggaggat gctggagccc gacgaggccg
     2461 cgcccatgat gaacatcccg gccagggaag agcgcccggc caggatcccc cacaggacaa
     2521 gaagggccaa gaggacggcg gccggtccca aaatcttttc cgggaagcgg ccgagccacc
     2581 accggatgtg gctgagatag gccgccggtt tcaagtcggc gttcggcgcc gcctctctga
     2641 tcaccgcgtt gacctgttcg atccgggtct tgaacagctc ttcatcgaaa tagcccgggc
     2701 ggacgtaaag attggcgatc ccgcgccggg cgagcccgga caggatgtcc gtcgtcagcg
     2761 gccggccgga catcaccaga acgctcatct cccccggaaa gaccgccgtt tccgcgaagc
     2821 atttccgggc cgcggcggcc acggagccga gcgaggcggc cgcgctttcc gaaacgtaat
     2881 tggccgtgcc gggaagcacg gccgcggcga ccccgccggg cgccaggacg ctcttcaccc
     2941 gcgcgaaaaa ttcggccgtg taataacggt tgagctggag attccacgga ccgggcaggg
     3001 cgatcaacac ggcgtcgtag cggacgccgg ccgtctcgat ccatcggaac ggcgttccgc
     3061 ggatcgggag gaccccggag gccgaaggag ccaagccgag ctttccggct aggcggaaca
     3121 agtgcggatc cggctcggcg atgtccactt ggccggctcc gtacttggcc agctcggccg
     3181 gcagtccgga gagcagcccg ccgatcaaca ggacgcggtc cgggtgctcc gtctggatca
     3241 gagcgaaggc cgccagctct tcgttaagca tcgcgttgcc cgaggcgtgg acaagaaccc
     3301 cgttttcata gacgttgact tggtcgcccg atcgggtgac ggtcagccgg cccgccccgc
     3361 ccgagaccgt gccgacgagc cgctcgttcg gatggaagcg ctggatcatc cacgcgtctc
     3421 cgccggcggt gataaacaaa acggccgcgg ccagcaggac ggccgggaat acccaccagg
     3481 ctcggttttc agaccttccg gcggccgcca gaagaaaaaa caagcccgag gaaaccgcaa
     3541 acacgatcgc caacgagggc aggccgggaa ggacggccgc ggcgcaaaac actccgccgg
     3601 ccgctccgcc gacgctctcc cagccgtaaa ccgtccggag gcttcggcct tcgcgccgca
     3661 ggacccgggc cgtccacgtg aaggagaatc cgttgagaaa gcagtacgga agcaggagaa
     3721 gggccagaaa tccgctcatg gccggcggtc cggcctcgat cccgggggcg aagaggaggc
     3781 ttttcagcgg gaacatcaag gggaagacag ccaaggggac gaccagaagc gccttgacca
     3841 gcgtcgaagc ccttttcacc gcttctcttg acgaggccgc gtccggaacc tgggcccggg
     3901 cgccggtcaa cgatccggct cccgatagaa gaagccaaag gaagatgatg agcgagagcg
     3961 tcagctcgtt gccttcaaac aacgtcagga attcccggat taaaagcgtt tggaccgcca
     4021 cgccggagaa gccgacagcg aagggaagcg ccaggtcccg ccgggcgcgg gccgcggttg
     4081 gagacggtcc cgcggagatg gacgcggcga tcgggaccgg acgcgttttc cgccgcttga
     4141 agaaataggg ggcgtgccaa caaaggtgaa acaggccgat aaggctcatc cccagcccga
     4201 agttgacgtg gattttcagc gctttttcgg cccaggggat ttccagccgg tagttgacct
     4261 gaacagccat caagacgccg aggccggcgg tgacgaggaa gaccgccagc agggcgtagt
     4321 tccagatttt cctgtgggcc ggcgtcgaga gaatgccgag ccggctgaac agaaaactga
     4381 tcccgtagag aagggctaaa aagagcccca ggggaatcag tccgtaaggg gcgttcatcg
     4441 tccctcagcg cccggattct tcaaatatca gcgcctcgta aacataaatg tccgcctgct
     4501 tccagccgtt ccagccgatc ccagccttgt cctgggcgca gtgccccagg aattcccgca
     4561 aggtccagcc ggtttcggcc gccacctggg gaagaaaggt cccgtgttga tttccttttt
     4621 cgatataaag gccgtgcttg ccgagttcga tctccttgat attcttgatc ttccgcctgg
     4681 gagtgagaac ggagatctcg atccggattt tatcgagctc ctcgggacgg acgggcggga
     4741 agcggatgtc ccggacggcc gaggcggcgg cgatctcctg gacgacttgg aataaaggct
     4801 tccgcggctc gaacgtcccc aagcatcccc gcaggtcgcc gttctttctc agggtgacaa
     4861 aggcgcccgc cggttcccgc aggatcggag cgtattgccc gggagtcagg ccggggagcg
     4921 gctcggcctt ggccgcggct tcgaccgacg cccgggcgat ccgtaaaaga acctcccggt
     4981 cctcggccga aagctcgaac ttcttttcct ctcgttcgga gacggcgatc gcctcatagc
     5041 cgaccacccg ggacttgtcc ccgtagggtt cgacgtcgcc ggaattcttg gagtcgaccc
     5101 gggtgaattc caggtccgca accccttccg tgagatagag aagcgtcagg gccgccggcc
     5161 aggcgcacat gctcgtgacc aggccgggga gcccgagacg ggcgttggac cgtaggacat
     5221 cgagaaaccg gtccggatca ttggcgcaga atccctcggc cgtcgccgcg tcggctttcc
     5281 gggcgtcatc ggcggaaggg tagtgggaaa aatcggcgct gacgacgaac aggttgtcct
     5341 tcgtgaaata cggtttgagg gcctcggcga tttttcggca gacggagggc gtctgggtcg
     5401 ccaggacgat gggcacgatt ttgaacggct tgttcaagcg gacctggagg aacggcaact
     5461 ggacctcgac gctgtgttcc tcccggtgcg cttcgggatc gcccgtaaaa caggaccgcg
     5521 tcccggacag gacggccgtg acctcccgat ccacctcgac ccgccccaaa ggcgtgagat
     5581 aatctccctc ggtgaagacc gaggccttgc ccaggtcgag ccggtgggac gaggccagga
     5641 tgaagacccg ggcataggga taatcggctt ggacttggtt gtagcccgag gcggccacgc
     5701 tccccgaaaa aacgtatccc gcgtgaggaa cgaccagggc ccggacggtc cccggaagcg
     5761 ggcctttttg gccgttttcc agaaaagccg agaccatgtt ccggagagcg gcggggtcgg
     5821 ccgggtaaaa cgacccggcg accgccggtt tccggtccgt atcgtccaac ggcgcgttca
     5881 tccggccgaa tgcgggaaaa acgaggacga agacaaaacc aagcagggcc gggacgatct
     5941 cgagacgcat cttcataaat cgagattaag cccggcccgg ccgcctgtca aggcattcgc
     6001 ccgcggcccg cgttcttttt ctcccgcttt attcatgaaa atccggtctt cccctccgcg
     6061 cctggcggcg aaagccgcat tcccgccggc ggaacggatt cataagcgcc cggcccggcc
     6121 gacggcccgg acgccgcggg gcgtcaacct cgttttcgat tcctctcccc ggccgagaga
     6181 accgcggccc tctcgattcg tggataatgc aggttattta tccgagcgtc ttttcttgtg
     6241 cccgaagatc cctccgccga ggaagagccc ggctccgatg acgaagccga agttgtacca
     6301 tccgccgttg ttgtggacct cgtaaaggcg gacggtcttg ctgaagatcg aaatgatgaa
     6361 ggtgaccggc gagatgaggc cgtgccacag gccgagccag aagccggcga ctttcccctc
     6421 ggtgtcgggc gttttctcgg cggtgttcgg tccgggcgcg cagctccagg cgcaaaacac
     6481 ggccagggtt gcgaggacga cggcggcggt cgcttttttc atgggccctc ctctttttct
     6541 tttttcatca taagcggacg gcggaccggc gtcaatatcc gacgggaaga ggcgcgcgtt
     6601 gaccggccgc ggcccctccc ttatactggc ttcgaggaag gaacccaagc cgcggagggg
     6661 aagaaaaccg gcgttccgtt cgtctcaagg acgccgcggg acgcgcggat catgtcggac
     6721 cgatgaaaac gccaagccgg gaagcggcgg ccggagaacg gagggaacgc ctcttctggg
     6781 gagccttcca tatcctcatc ctggctttcg ccgtctggct ggtttccccg ttcatcgtca
     6841 tctcctcctc cgccttttcc tcccgccaag gatacggcgt tcgcctcgcc ctcgggcttt
     6901 tgatcttcct catgtatgtg gggaaatggt cgttcgacgt cctcgccccc caaggcttgg
     6961 cccgccgggt ttcccggctt aaaaccgccg ccttaatcgt ttacatcgtc atcgtcatca
     7021 gcttcatgat ctacatcgtc gcccaggcgg cttcgctcta tctgcggacg gccgtcgacg
     7081 agtcggcgga caatccggac caggtcatca cccgcctgga acgggtcgaa aatccggcct
     7141 tcggcccatc cgcatttttc ccttgaacca agaggaaaaa caggcgatcg ggcggggccg
     7201 gaaggcgagc ggttcccggc ccttgattta tccccgaaaa tgagtatagt ttccgcattc
     7261 cgtctttttt gggatggcgg aaaagaagaa gaaaaggagg aaaacatgag aaaaatcacg
     7321 ctgttattgg cgctcgggct gctggtcctg ggcaccgccc aggcggccat catcacgaac
     7381 accaaccaga gcacggcata catccggatg ctgtcgcgga acgcgacgac cgagatcgat
     7441 gccgtctact acaacccggc cggcttggtc cggctggccg acggctggca tttctccctg
     7501 tccaacgaga cgatctccca gactaagacc gtcgtcaacg acttcccgct gctcaataac
     7561 ggaacctacg agggcaaggt cagcgtcccc atctatccgg acgcttacat cgcctggaaa
     7621 aagggaaaga tcgccgtgtc ggtcggattc ggccccaacg ccggcggcgg ctcggccgat
     7681 taccagaccg ggcttccctc gttcgaaacg gggatctcca tgcttccgac cttgattacg
     7741 atgatgggcc tgccgacgac caagtattcg gccgacatcg ccttcaaggg gagctcggtg
     7801 ttcctcggat tccagggcaa cctctcctac gccttgagcg agaccttctc cctggccgcc
     7861 gggttccgct atatctcggc gaccaacacg tatgagggcc ggctgaaatc ggtgatgatc
     7921 aatccccagc atcccttgct gaatccctcc ggcgggatga tgtcggcggt gacgttcttc
     7981 acggccatcg gccagccggg cttg
//
LOCUS       DAOOXH010000101         7948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_10178_length_7948_cov_7.604966, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000101 DAOOXH010000000
VERSION     DAOOXH010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7948
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10178_length_7948_cov_7.604966"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PKV64_09910"
     CDS             complement(<1..407)
                     /locus_tag="PKV64_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPN17426.1"
                     /translation="MKKIDALIANIRKVFVGDPEVVRLVVTALLARGHLLIEDVPGIG
                     KTLLGVALARSIESSFRRIQFTNDLLPSDLLGMSIFNPRESTFSFKPGPIFSHIILAD
                     EINRTTPKTQSALLEAMNDLQVTVDAGPYPLPDP"
     gene            673..1587
                     /locus_tag="PKV64_09915"
     CDS             673..1587
                     /locus_tag="PKV64_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17427.1"
                     /translation="MNKRHSFAGRAVLGLALAVVLSAPAPAQEPAASGLLPRVDGWEA
                     SEPPQTFLPDTLFEYINGASENFLSYGFVELALGRFQSAGGVEMTVEIYDMGASDNAF
                     GVYGSERYPDSRFLPVGGQGYLEEGSLNFFAGRFYVKLLSYPAGEGTEEALRSFAAAV
                     LKGIPDPGDFPLPVRAFPKSGLVENSEKFILRDFLGMSFLSRGFVAAYRTAGGEEFEA
                     LIIEADDPAEAGEMRSALMERLSAAGGASPEEDGLVRLKDPYLADILIAPAGRFLCGA
                     MKIRDGAAEAEKAAEAVVKEMVRALAGK"
     gene            complement(1610..1960)
                     /locus_tag="PKV64_09920"
     CDS             complement(1610..1960)
                     /locus_tag="PKV64_09920"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HPN17428.1"
                     /translation="MSPNPNDAPPPINRAEALERVGDDASFLEELLAIYDEEFTEKSP
                     ALEEALNRADARAVREIGHYLKGASANLSLPGLQAAAWAIEKAGEENRLEDANAAYRT
                     LLEEYDRLKAFLAR"
     gene            complement(1941..2954)
                     /locus_tag="PKV64_09925"
     CDS             complement(1941..2954)
                     /locus_tag="PKV64_09925"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPN17429.1"
                     /translation="MAAPLSILIAEDDPISGRILEKNLKSWGYDVSLAKNGNQAWEVL
                     RSPNTRMALLDWMMPGAEGPELCSRIRALNKPGYTYIILLTSRDSTQDIIDGLEAGAD
                     DFMTKPVHFAELRARLQTGSRIIALEDKLLESQKRLFELATRDSVTGIWNRATILRFL
                     SDEMAHCERGGGYSLSLILIDIDFFKKINDTYGHQVGDRVLSRITATLEKHIRPYDKV
                     GRYGGDEFLIVLPNCSLYHVAVVAERLRAAVKKIRMRIEGATLNLSLSLGCASSECFR
                     RPSCDRLIQESDTALYRAKKAGRNRVRLCAELKTPPDAKASRAVMRHTSPKKGIRRVS
                     KSK"
     gene            3129..4157
                     /locus_tag="PKV64_09930"
     CDS             3129..4157
                     /locus_tag="PKV64_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPN17430.1"
                     /translation="MSKDGRALTIAAWIVAATVIGFVFKSAKTVLFPLFLALFFYFIL
                     APLQDLLMKIKFSRTLSLILIILFAFVVLFLMAQLFYSSGKALASDLPDYGNKLKDML
                     DELKNSIELPGGGTFDPLAWVDSLDVAKVGAFLLNSLGTFVSFVSAIFLVLIFLIFML
                     AGRGKLNDKIRRFSPHPRRSDQIIEIVGNIDRQVQKYLVIKTAISVASGVISLVVFLA
                     FGLRYAILWAFITFVLNYIPNIGSFISKILPFGFSILQFGRVWPAVWMLAILVVTDAI
                     TGSFIEPRVMGKGLGLSPLAILISLFFWGWLWGIPGMILAVPIMVVMKIIASNFESLR
                     WFEALLSK"
     gene            complement(4164..4925)
                     /gene="rnc"
                     /locus_tag="PKV64_09935"
     CDS             complement(4164..4925)
                     /gene="rnc"
                     /locus_tag="PKV64_09935"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPN17431.1"
                     /translation="MLKKFFKKTQNRDHALEKRLNYRFKDQSLFQMALTHSSHAYETQ
                     DHPLDNERLEFLGDSVVGLIAADFFYAMYPEANEGDLSKFKSSASSTVALADYARQIK
                     LDEVIHLGKGEEKSGGRKKSTILAGSFEALIGAIYIDGGFDAARTVYERLLEGSYAKV
                     KKQGHEINNYKSALQEFLQKEDLPAPQYKMVTAAGPDHRKEFVVEVIVNKTVLGRAKG
                     PSRKVAEQKAAENVLKSFFGKRIKSLTPETFLFKS"
     gene            complement(4919..6130)
                     /locus_tag="PKV64_09940"
     CDS             complement(4919..6130)
                     /locus_tag="PKV64_09940"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPN17432.1"
                     /translation="MDGGKIASAGGRIEAPGADVIDASGLVVAPGFIDMHVHLREPGQ
                     TYKETIISGARAAARGGFTAVCAMPNTSPVNDSPEVTAWIREAAAGAVIRVFPIAALT
                     RGQKGGEPVDFDALLEAGAVAFSDDGRCIQDEDLMRRVLEAARARDALVIDHCEDRAL
                     FGDGVINDGPIARRLNLPGIPAAAEEAIVARDIALAGRTGGRVHLAHLSTKGSVELVG
                     EAKRRGLPVTAEVTPHHLLLTEDDVEAGGPNFKMNPPLRSAADAAALAAAVRDGIIDV
                     FATDHAPHAAAEKAAGLRDAPFGIVGLETAIPLLLDRLVGRGVISLLRFAAMGSLRPA
                     EILRWPDKGRLAPGAAADVTILDLERTVTVDASRFASLGRNTPFDGWTLRGAPVMTIV
                     GGRTVFSEMPC"
     gene            complement(6235..6798)
                     /gene="pyrR"
                     /locus_tag="PKV64_09945"
     CDS             complement(6235..6798)
                     /gene="pyrR"
                     /locus_tag="PKV64_09945"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HPN17433.1"
                     /translation="MTKKVKASVMDAVKIKRVISRMATEILERNRDLKNLVIAGIPTR
                     GIVIAKRIAKVIKDLEKTEIPVGVIDITRFRDDGAASPAGPAAGKSKTRLAVDKKDVI
                     LVDDVLFTGRTIRAAMEALFELGRPKSIQLLVLIDRGHRELPIRADYVGKVLPTSRRE
                     VVRVGVKEIDERDDVLITEPVALRRKR"
     gene            complement(6838..7764)
                     /locus_tag="PKV64_09950"
     CDS             complement(6838..7764)
                     /locus_tag="PKV64_09950"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPN17434.1"
                     /translation="MRIFRSLDAVTPPRRGLALAVGNFDGLHRGHRRIIRRLSREAEA
                     AGLPVVLLTFAPHPEKIFSPDRLLMIQTLDQRLRALCKLGVESVAVVPFTKSFARMSA
                     DSFASGVLSSALKARLVVVGADFRFGAKRRGDAAFLEAAGRRLGFRTIAVPPLLEEGA
                     PVSSSRIRTTLLSGNVEEAARLLGRPYEIEGDVVSGSGRGRTLGFPTANLASPNEILP
                     PGVFATRLFRKGKTWNSVTNVGRRPTFGASEPSVETHILDASPLLYGAYLRLTFVARL
                     RDERVFRSSRALAGAIRADIAAARRIFGRFGL"
     gene            complement(7769..>7948)
                     /locus_tag="PKV64_09955"
     CDS             complement(7769..>7948)
                     /locus_tag="PKV64_09955"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HPN17435.1"
                     /translation="PPKNAPDIDLPQARRLIDLLDLLADRTKGRLEPEEEKFLEFVLS
                     ELKMQYLEKTETVKL"
BASE COUNT         1313 a         2404 c         2652 g         1579 t
ORIGIN      
        1 gggtcgggca ggggataggg tccggcgtcg acggtgactt ggaggtcgtt catcgcctcc
       61 aggagggcgc tttgggtctt gggggtcgtc cggttgatct cgtcggccag gatgatgtgg
      121 ctgaagatcg gacccggctt gaaggaaaac gtgctctccc gggggttgaa aatcgacatc
      181 cccaggaggt cggatggcag gagatcgttg gtgaactgga tccggcggaa ggaggattcg
      241 atcgatcggg ccaaggccac gcccaggagc gttttaccga taccgggaac atcctcgatc
      301 agcaggtgcc cgcgggccag gagggcggtg acgaccagac ggacgacctc cgggtcgccg
      361 acgaagacct tccggatgtt ggcgatcagg gcatcgatct ttttcatgag gattcattat
      421 aatccttttg atttaggggc gcatccccct caacgggccg ttttcactca acctcgcctt
      481 ccccctcgct catgccccct tttgtatctc tttccctccc gtgtcttccc cctcgcggcc
      541 cgcctccgcg gcggtgccgg ctcggcgatg attccggagg cgcgatcggc ggccggaaca
      601 gaaaggatcg ccttgcttga aaggaggtat tgaggggttg agcggggcgc cgcctcatca
      661 taaaatatgg acatgaacaa acgacattct ttcgccggac gagcggtttt aggattggcc
      721 ctggccgtcg ttttatcggc cccggcgccg gcccaggagc ccgcggcctc gggcctgctt
      781 ccccgggtcg acggctggga ggcctccgaa ccgccgcaga cattcctccc cgacaccctg
      841 ttcgagtaca tcaacggggc gtccgagaac tttttgagct atgggttcgt cgagctggcc
      901 ctgggccggt ttcaatcggc ggggggagtg gaaatgaccg tcgaaattta cgacatgggc
      961 gcgtccgaca atgccttcgg cgtttacggc tcggagcgtt accccgacag ccggtttctc
     1021 cccgtgggcg ggcagggcta cctcgaggag ggatcgctca attttttcgc cggccggttc
     1081 tacgtcaagt tgctttctta tccggccgga gagggaaccg aggaagccct gcggtcgttt
     1141 gccgcggctg tcctcaaggg catccccgat cccggggact ttccgcttcc cgtccgggcg
     1201 tttccgaaaa gcggcctggt tgaaaacagt gagaaattca tccttcggga tttcctcggg
     1261 atgtcttttc tttcccgcgg gtttgtcgcc gcttaccgga cggccggcgg cgaggaattc
     1321 gaggcgttga tcatcgaggc ggacgatccg gcggaagccg gggagatgcg gagcgctttg
     1381 atggagcgct tgtcagcggc cggcggggct tccccggaag aggacggtct cgtccggctg
     1441 aaggatcctt acttggcgga catcctgatc gccccggcgg gacgctttct ctgcggagcg
     1501 atgaaaatcc gggacggggc ggccgaggcg gagaaagcag ccgaagccgt cgtcaaggag
     1561 atggtccggg cgctcgccgg caagtgattt tcggggcccg ccggagaggt caccgggcca
     1621 ggaacgcctt caggcggtcg tattcctcga gcagcgtccg ataggcggcg ttcgcgtcct
     1681 cgaggcggtt ttcctcgcct gctttttcaa tggcccacgc ggcggcctga aggccgggca
     1741 ggcttaaatt agccgaggct cccttcaagt agtggccgat ttcccggacg gcgcgggcgt
     1801 ccgcccggtt caaggcttcc tccaaggcgg gcgatttctc ggtgaattcc tcgtcgtaga
     1861 tggctagcaa ttcctcgagg aacgaggcgt cgtcgccgac gcgttccagg gcttcggccc
     1921 ggttgatcgg cgggggggcg tcatttggat ttggagacac ggcggattcc tttcttaggg
     1981 gaggtgtggc gcatcacggc gcgggacgcc ttggcgtccg ggggagtctt cagctcggcg
     2041 cagagcctga cccggttgcg gccggctttc ttggcccgat agagggcggt gtcgctttcc
     2101 tgaatcaggc ggtcgcagga aggccggcgg aagcactcgg acgaggcgca acccagggac
     2161 agggagaggt tcaatgtcgc gccttcgatc cgcatcctga tttttttgac cgccgcccgc
     2221 agccgctcgg cgacgacggc cacgtggtaa aggctgcaat tcggcaagac gatcaggaac
     2281 tcgtcgcctc cgtagcgtcc gaccttgtcg tacggccgga tgtgtttttc gagcgtcgcc
     2341 gtgatcctgc tcaggacccg gtcgccgact tggtggccgt aggtatcgtt gatcttcttg
     2401 aaaaaatcga tgtcgatgag gatcaggctg agcgaatatc ccccgccgcg ctcgcagtgg
     2461 gccatctcat cgctgagaaa gcggagaatc gtcgcccggt tccaaatgcc ggtcacgctg
     2521 tcccgggtgg ccagctcgaa caggcgtttc tgggactcga gcagcttgtc ttccaaggcg
     2581 atgatccggc ttcccgtctg gagccgggcc cgaagctcgg cgaaatggac cggtttggtc
     2641 atgaaatcgt cggctcccgc ctccagcccg tcgatgatgt cctgggtgct gtcccgggac
     2701 gtcaagagaa tgatgtaggt gtatcccggc ttgttgagcg cccggatccg gctgcagagc
     2761 tccgggccct cggctccggg catcatccag tcgagaagag ccatccgcgt gttcgggctg
     2821 cggagaacct cccaggcttg gttgccgttc ttggcgaggg agacatcata accccaggat
     2881 ttgaggtttt tttccagaat gcggccggag atcggatcgt cttcggcgat gagtatcgaa
     2941 agcggcgcgg ccatcttgcg gagccctaaa aaatgctctc tttgcttgaa aaaacgcctc
     3001 cgagccgtct cggagaatcc atcgtccttc attataacca aggcgccgtc caggtcaatg
     3061 ccccgtgagg gtgatggagg gaaaaaaagg actttacccg gccgctggaa gtccgtagaa
     3121 tagggcccat gagcaaggac ggccgggcgt tgaccatcgc ggcctggatc gtggccgcga
     3181 cggtcatcgg ttttgtcttc aaatcggcca agaccgttct ttttccgctc ttcctcgcct
     3241 tgttttttta cttcatcctg gcccccctcc aggacctgct gatgaagatc aagttctccc
     3301 ggacgttgtc cctgatcctg atcatcttgt tcgccttcgt cgtcctcttt ctcatggccc
     3361 agctcttcta ttccagcggg aaagcgctcg cctccgacct ccccgactat gggaacaagc
     3421 tcaaggacat gctcgatgag ctgaaaaaca gcatcgagct ccccggcgga ggcactttcg
     3481 atcccctggc ctgggtcgac agcctggacg tggccaaggt cggggcgttc ctcctgaatt
     3541 cgctggggac gttcgtctcg ttcgtctcgg cgatcttttt agtcctgatt ttcctgattt
     3601 tcatgctggc cggccggggc aagctgaacg acaagatccg ccgcttctcc ccgcatcccc
     3661 gccgctccga ccagatcatc gagatcgtcg gcaacatcga ccgccaggtc cagaaatacc
     3721 tcgttatcaa gacggccatc agcgtggcgt ccggggtgat ctcccttgtc gtcttcctgg
     3781 cgttcggcct ccgttacgcc atcctctggg ccttcatcac cttcgtcctg aactatatcc
     3841 ccaacatcgg ctccttcatc tccaagatcc tgccgttcgg cttctccatc cttcagttcg
     3901 gccgcgtctg gccggccgtc tggatgctgg ccatcctggt ggtgaccgat gcgatcacgg
     3961 ggtcgttcat cgaaccccgg gtcatgggga aaggcctcgg cttgagcccc ctggccatcc
     4021 tgatctctct ctttttctgg ggctggctgt gggggattcc gggaatgatc ctggccgtgc
     4081 cgatcatggt cgtgatgaag atcatcgcct cgaacttcga atcgctgcgg tggttcgagg
     4141 ccctgctgag taaatagccg ggcttaggac ttgaagagga acgtctccgg cgtcagggat
     4201 ttgatccgct tgccgaaaaa gcttttaagg acgttttcgg ccgctttctg ctcggccacc
     4261 ttgcgggaag gccccttggc ccggcccagg acggttttgt tgacgatgac ctcgacgacg
     4321 aattccttcc ggtggtcggg gccggcggcg gtcaccatct tgtactgggg cgcggggaga
     4381 tcctccttct ggaggaattc ctgaagagcg gacttgtaat tattgatttc gtgcccctgc
     4441 ttcttgacct tggcgtacga tccctcgaga agacgttcat agaccgtccg ggcggcgtcg
     4501 aagccgccgt cgatgtagat ggccccgatc agggcttcga acgatccggc caggatcgtg
     4561 cttttcttcc gtccgccgct cttctcctcg cccttcccga ggtggatcac ctcgtccagc
     4621 ttgatttgcc gggcgtaatc ggccagggcg accgtgctgg aggcggagga cttgaacttg
     4681 gacaggtctc cctcgttggc ctcgggatac atcgcgtaga agaaatcggc cgcgatcagg
     4741 ccgacgaccg aatcgcccag gaactcgagg cgttcgttgt ccaggggatg gtcctgcgtc
     4801 tcgtatgcgt gggagctgtg agtcagagcc atctggaaga gagactggtc cttgaagcgg
     4861 tagttcagcc gcttttccaa ggcgtgatcg cggttttggg ttttcttgaa gaattttttc
     4921 agcatggcat ttccgaaaag acggtccgcc cgccgacgat ggtcatcacc ggggcgccgc
     4981 ggagcgtcca gccgtcaaac ggcgtgttgc ggccgagcga ggcgaaccgg gacgcgtcga
     5041 ccgtgacggt tcgctcgaga tccaggatcg tcacgtccgc ggccgctccc ggggcgaggc
     5101 gtcccttgtc cggccaccgg aggatttcgg ccgggcggag cgatcccatc gccgcgaacc
     5161 ggagaagcga aatcaccccc cggccgacca gccggtctaa aaggagcggg atcgccgttt
     5221 ccagcccgac gatgccgaag ggggcgtccc gcagcccggc cgctttctcg gcggcggcgt
     5281 gcggggcgtg atcggtggcg aaaacatcaa tgattccatc ccggacggcc gcggccaggg
     5341 cggccgcgtc ggccgcgctc cgcagcggcg gattcatctt gaaattcggc ccgccggcct
     5401 ccacgtcgtc ctccgtcagg aggaggtgat ggggcgtgac ctcggccgtg accggaaggc
     5461 ctcgccgctt ggcctctccg acgagttcga ccgatccctt ggtgctgagg tgggcgagat
     5521 gaacgcgtcc gcccgtccgt ccggccaggg cgatgtcccg ggcgacgatc gcctcctcgg
     5581 ccgcggccgg aatgccggga agattcagcc gccgggcgat cggcccgtcg ttgatcactc
     5641 cgtctccgaa caacgcccgg tcctcgcagt ggtcgatgac cagggcgtcc cgggcacggg
     5701 ccgcttcgag cacccggcgc atgagatcct cgtcctggat gcatcggccg tcgtcggaga
     5761 aggccacggc cccggcctcg agaagggcgt cgaaatcaac cggctccccc cctttctggc
     5821 cgcgggtcag ggcggcgatg ggaaagacgc ggatgacggc tccggcggcc gcctcgcgga
     5881 tccaggcggt cacctcgggc gaatcattga ccggggaggt gttgggcatg gcgcagacgg
     5941 ccgtgaatcc gccgcgggcg gcggcccggg cgcccgagat gatcgtctcc ttgtaggtct
     6001 ggcccggctc ccgaagatgg acgtgcatgt cgatgaaacc aggggcgaca acgaggccgg
     6061 aggcgtcgat cacgtcggcg ccgggggctt cgatccgccc gccggccgag gcgattttcc
     6121 cgccgtccaa aagaagatcg ccgacggcgt cagttccgga gtaaggatcg atcagccgtc
     6181 cgtcccggat caggcgcttg gcgttgctcg tgataacctc cgcggtcgag accctcagcg
     6241 cttgcggcga agggcgacgg gctcggtgat caagacgtcg tcccgctcgt cgatttcctt
     6301 cacgccgacc cggacgacct cgcgccggga ggtgggcagg acttttccga cgtaatcggc
     6361 ccggatgggc agctcccggt ggccgcggtc gatcaggacg agaagctgga tgctcttggg
     6421 acggccgagc tcaaagaggg cttccatggc cgcccgaatc gtccggccgg tgaacaggac
     6481 gtcgtcgacc aggatgacgt ctttcttgtc gacggcgagg cgggtcttcg attttccggc
     6541 cgccggcccg gcgggggagg cggccccgtc gtcccggaaa cgggtgatgt cgatgactcc
     6601 gacggggatc tccgtttttt ccagatcctt gatgaccttg gcgatccgct tggcgatgac
     6661 gatgccgcgg gtcgggatgc cggcgatcac caggtttttc aggtcgcggt tgcgctcgag
     6721 gatttcagtg gccatgcggc tgatgacgcg cttgattttc accgcgtcca ttacgctggc
     6781 cttcactttt ttggtcatta caatctcctg acgccgcctg ttataccata agaaaattca
     6841 aaggccaaac cgtccgaaaa tcctccgggc ggcggcgatg tcggcccgga tggctccggc
     6901 cagggcccgg gagcttcgga aaacccgctc gtcccgcagg cgggcgacga acgtaagacg
     6961 gaggtaggct ccgtagagga ggggggaggc gtccaggata tgcgtttcca cggacggctc
     7021 cgaggctccg aaggtcggcc gccggccgac gttggtcacg gaattccagg ttttcccctt
     7081 gcggaacagg cgggtggcga agacgcccgg gggaaggatt tcgttgggcg aggcgaggtt
     7141 ggccgtgggg aagccgagcg tccggccgcg gccggatccg gaaacaacgt ctccctcgat
     7201 ctcgtagggg cggccgagaa ggcgggcggc ttcctccacg tttccggaca gcagggtcgt
     7261 ccggatccgg ctgctgctga ccggagcgcc ctcctcgaga aggggaggga cggcgatcgt
     7321 ccggaagccg aggcgtcggc cggcggcctc aagaaaagcg gcgtcgccgc gccgcttggc
     7381 cccgaaccgg aaatcggccc cgacgacgac caggcgggcc ttcagggcgg aggataagac
     7441 gccggaggcg aaggaatcgg cgctcattcg ggcgaaggac ttggtgaagg gaacgacggc
     7501 cacggattcc accccgagct tgcagagagc ccggagccgc tggtcgagcg tctggatcat
     7561 caacaagcgg tcggggctga agatcttctc gggatggggg gcgaaggtca ggaggacgac
     7621 gggaaggccg gcggcttcgg cttcccggga aaggcggcgg atgatccgcc ggtggccgcg
     7681 gtggaggccg tcgaaattgc cgacagccag ggccaagccc cgccgggggg gagtgacggc
     7741 gtccagcgac cggaagatgc gcaccggctc agagcttgac ggtctcggtt ttctcgagat
     7801 actgcatctt aagctcggag aggacgaatt cgaggaattt ttcctcctcg ggctcgagcc
     7861 gtcccttggt ccggtcggcc aggaggtcga gcaagtcgat cagccggcgg gcttggggga
     7921 ggtcgatgtc cggggcgttc ttgggcgg
//
LOCUS       DAOOXH010000102         7837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_10390_length_7837_cov_7.077358, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000102 DAOOXH010000000
VERSION     DAOOXH010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10390_length_7837_cov_7.077358"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="PKV64_09960"
     CDS             <1..1047
                     /locus_tag="PKV64_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN17436.1"
                     /translation="PSYGLLVIDRDKPVAVDGGRETSAGRDLTAAWTQAAYPDGKPYF
                     KVRTVDSIEDGKRRLKNRDADLLIVIPEEFSAFIAESGDRPGETARTAPVKNIGDESG
                     ARFMVAAALADFITYSYVAAAKKIDLPAAVEFEGLGSGRVPNDFDLYVPALLALAIIM
                     IMFTAAASLIKEVDKGTIVRLALSRLNPWEYLGAIGLIQVLIGTFALALTFLSALSVG
                     YRTGGSIGLFLLVGALTTVSVVAISLIVAGWMKTIYELLTVGVFPFFVLMFFSESMFP
                     LPKILLLNFGTQAFYANDVLPTALAVKAFNKILNFQAGLADIGFEMMAILVLTIIYFL
                     IGLWLFRRRHLRPQ"
     gene            1351..3165
                     /locus_tag="PKV64_09965"
     CDS             1351..3165
                     /locus_tag="PKV64_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPN17437.1"
                     /translation="MQAIVLAAGMGKRLGALTRNSTKCMVKVGGKRLIEHALEALSSQ
                     GIRKAVIVTGHGAEELRAVVGASCAGVAVSYIHNPDYARTNNIYSLLLAREAMEAEDS
                     LLLESDLIFEPAIIADCLRHPSANTAVVARFMPWMDGTVTRLEDNHDITCFLGKKEMV
                     EADLAHYYKTVNIYKLSKDFCRDKFFPFLDVYIRAKGLNEYYEEVFKVLTFIDRGALR
                     AVDVSRRLWYEIDDIQDLDIAEALFARGKAKVEALHARYGGYWRFPGLTDFCYLVNPY
                     FPTPPMFAEMSRSFPVLVANYPSGQRVQNLLAAKMFGIAPHHILTGNGASELIRAMFR
                     GESGAVGLSVPTFDEYRASAGPERIKPFRPVRTDFRYTAGELGDFCLREKVSALILIN
                     PDNPSGNFISRGEVLRLAERLAEKNIRLIYDESFLDFADGSADHSLIEGSVLERLPNL
                     AVVKSISKSYGVPGLRLGVLASSDRALLERVWKEISIWNINSPAEHFLQIIDKYGTDF
                     RAACRRIAEERERFVRALGEIPFLRVLPSAANYVLCEILPPWTPAALTEILLNEHDIL
                     IKDASGKLGFDGRPFVRLTVRDETDNDRLLAALKALIR"
     gene            3175..4065
                     /locus_tag="PKV64_09970"
     CDS             3175..4065
                     /locus_tag="PKV64_09970"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPN17438.1"
                     /translation="MLRAWFLEYFRSLKRPDVEEWADLFLFRPLAFLLVKAIYRTSLT
                     PNQLSVFSMVLGVAAGILLGTGGPAAAVVVVVLLFLSVVVDCADGQLARLKKNGTHMG
                     RVVDGAIDYVVGAAMYLGLGFGFASALDNPVRGWLLVAAAAASNIIHSVVFDYYRNRY
                     LAYVHGVSSAQTDDRNAFRREYVELGRRGGQELRRKFIRLYLAYIAFQMKISRDPMTL
                     GPVEPEIFRKTNRLAMRLWTFLGSSTQAVLLMIALLGGRLLLYYWAMIVVLNGMMFAL
                     FLGQSAVDLKVRSLAAEASK"
     gene            complement(4057..5187)
                     /gene="lpxB"
                     /locus_tag="PKV64_09975"
     CDS             complement(4057..5187)
                     /gene="lpxB"
                     /locus_tag="PKV64_09975"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPN17439.1"
                     /translation="MSDPAILIVAGEKSGENYGAGLVREFRLLYPRAEFFGVGGSNMA
                     DAGVDILVPMDRLAVMGLAEVVKDVPRIRKILRRLATEASARRPAAAVLIDSPDFNLR
                     LARRLVKAGVPVLYYVSPTVWAWRKSRLKTIKSVVRKMLLIFPFEERIYARAGIPHRY
                     IGHPLLERLRIRSSRDEFFRARGLDPARPLIALMPGSRENEIRRHLAVVLEAAVRLQS
                     VRRAQLVLIQNPDLDPALLPRLSVASKADILLLREDAYEAMAASDLVLSACGTANLEA
                     ALLGTPLVAFYKISPLTYAAGRPFVRIRDYSIVNILAGRRIVPELIQRRMTPDALVRE
                     ALRLLDDSGARLEMKDAFARIRAELGEKRASVNAARELAALL"
     gene            complement(5184..5822)
                     /locus_tag="PKV64_09980"
     CDS             complement(5184..5822)
                     /locus_tag="PKV64_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPN17440.1"
                     /translation="MVRPSEAGYTLLILMVFISVMTVALAIALPVLETQGWREKEEEL
                     LFRGRQYVEAVRLYADKNPGQYPKSIEELVKGRYLRKAFPEPMTREGKWNLILLPAQA
                     GELGAPGPKAPAARGKASRSRPAKKGEEPEDAGDFAAAEGEPSSLTQVMVVPEKLLSS
                     VDNPRIIGVVSPSGRKSFFIYDKNESYDTWLFFFGRTPGQNPEIIRFGTPLK"
     gene            complement(5816..6214)
                     /locus_tag="PKV64_09985"
     CDS             complement(5816..6214)
                     /locus_tag="PKV64_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPN17441.1"
                     /translation="MARKHERGYTLIEIIIVFTMIGILVGLALPEYRNAVKKAKEAAL
                     KEDLFQFRKLIDQYFSDKGKYPASLQALVDEGYLRKIPADPLTTRTDWVEVMEEPSAE
                     ELEPGKILGIVDVRSSSEAQALDGSIYNTW"
     gene            complement(6228..6737)
                     /locus_tag="PKV64_09990"
     CDS             complement(6228..6737)
                     /locus_tag="PKV64_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPN17442.1"
                     /translation="MRLRPGFTLTEMLVTAAILAVLAGVAVPVVKVTVQRDKEVELRR
                     ALRLVREAIDSYKRMADEGTIEVEEGGEADNYPPTLDVLVKGVPLAASTDEGDRGENG
                     RKLGSGKEKIFKFLRRIPVDPMTGTSEWGQRSNQDPPDATTWGGENVYDIYTKSRRTA
                     LDGSKYADW"
     gene            complement(6791..>7837)
                     /locus_tag="PKV64_09995"
     CDS             complement(6791..>7837)
                     /locus_tag="PKV64_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPN17443.1"
                     /translation="ELLLGRNGREDGLLGQGAVAETSILPAIPAEEQFPYTVRIVSDI
                     LESNGSSSMATVCGGVLALMDAGAPLTFVPAGIAMGLVKDGDRFVVLTDIAGIEDHFG
                     DMDFKVAGSEKGITAIQVDLKIDAIPLAVCREAMMKARAARLKVLDKMKEAIPRPRTE
                     ISPYAPRILFLYINPEKVGEVIGPAGKIIKKIVSTTKAKIDIDETGKVVIASVDMEAA
                     ERAKQMILDLTAEAEVGQTYTGKVVRIEEYGAFVEIMPNIVGLMHVSEIAPYHIRSVG
                     DVLHLGDVITVKVLTIEDNRIRLSMKALNPDAVPPEGTGDKGGSGHPDSRPRPPRSDR
                     DRGRGGKGGGRGRY"
BASE COUNT         1322 a         2481 c         2467 g         1567 t
ORIGIN      
        1 ccgagctacg gcttgcttgt tatcgaccgg gacaagccgg tcgccgtcga cggcggccgg
       61 gagacgtcgg ccggacgcga tctgacggcc gcctggaccc aagccgccta tccggatggg
      121 aagccgtatt tcaaggtccg gacggtcgat tcgatcgagg acggcaagcg gcggctgaaa
      181 aaccgggacg cggaccttct catcgtcatt ccggaggaat tctccgcctt catcgcggaa
      241 tcgggagacc ggccggggga aacagcccgg acggcgccgg tcaagaatat cggcgatgag
      301 tccggcgccc ggttcatggt cgccgcggcg ctggccgatt tcatcacgta ctcgtacgtc
      361 gccgcggcca agaagatcga ccttccggcc gcggtcgagt tcgagggact cgggtccggc
      421 cgcgtcccca acgatttcga cctgtatgtc ccggccttac tggcgctggc gatcattatg
      481 atcatgttca cggccgcggc ctcgctgatc aaggaggtcg acaaggggac gatcgtccgc
      541 ctggccctgt cccggctcaa tccctgggaa tacctgggcg cgatcggcct gatccaagtc
      601 ttgatcggga cgttcgccct ggccctgacg tttctttcgg ccctctccgt cggctatcgg
      661 accggcggct cgatcgggct tttcttactc gtcggagcgc tcacgacggt gtcggtcgtc
      721 gcgatcagcc tgatcgtggc cggttggatg aaaacgatct acgagctatt gaccgtcgga
      781 gttttcccgt ttttcgtctt gatgtttttt tccgagagca tgttcccgct tccgaaaatc
      841 ctcctcttga atttcggaac ccaggcgttc tatgccaacg acgtcctgcc gacggctctg
      901 gcggtcaaag ccttcaacaa gatcttgaac ttccaggcgg gattggccga tatcggattt
      961 gaaatgatgg cgatcctcgt cctcacgatc atctatttcc tgatcgggct atggcttttc
     1021 cgccgccgtc atcttcgtcc gcagtgaaag acaggcggga cgcggcctgt caattcggag
     1081 cctggtctta tccgaacctg tttatccatt tccctaattc gatccgccgc ccgatcctga
     1141 tttcctcccg ggccaaaccg aacctggtta tccatttaag caattccccg gatcagggaa
     1201 aaaaatgcgg aaatggataa acaggtaccg atagggaggg ccggaaacag ggaaatggat
     1261 aaacaggttc ggaattaacg gcggaagccg atttgaccct ctcggcgcgt tccgttaaga
     1321 tgttagatcc tttctaagga gccgaatcgc gtgcaggcca tcgtattagc cgccggaatg
     1381 ggcaagcggc tgggcgccct gacccggaac tcgaccaagt gcatggtcaa ggtcggagga
     1441 aagcgcctga tcgaacatgc cctggaagcc ttgtcgtccc agggaatccg caaggccgtg
     1501 atcgtcaccg gccacggagc cgaggagctg cgggccgtcg tcggcgcttc ctgcgccggc
     1561 gtggccgttt cctacattca caaccccgat tacgcccgga cgaacaacat ctattcgctt
     1621 ctgttggccc gcgaggcgat ggaggccgag gattccctcc tcttggaaag cgacctgatt
     1681 ttcgagccgg cgatcatcgc cgattgcctc cgccacccct cggccaacac ggccgtcgtc
     1741 gcccggttta tgccgtggat ggacgggacg gtcacccgcc tggaggacaa ccacgacatc
     1801 acctgcttcc tcggcaagaa ggagatggtc gaggccgacc tggcccatta ctacaagacg
     1861 gtcaacattt acaagctgtc caaggatttc tgccgggaca agttcttccc cttcttggat
     1921 gtctatatcc gggccaaggg cctgaacgag tactacgagg aagtcttcaa ggtcctgacg
     1981 ttcatcgacc gcggggccct gcgggcggtc gacgtaagcc gccggctctg gtacgagatc
     2041 gatgacatcc aggatctgga catcgccgag gccctgttcg cccgggggaa ggccaaggtc
     2101 gaggccctcc acgcccgcta cggcgggtac tggcggttcc ccggcctcac ggatttctgc
     2161 tacctcgtca atccctattt cccgacgccc ccgatgttcg ccgagatgtc ccggagcttc
     2221 ccggtcctgg tcgccaacta tccttccggc cagagggtcc agaacctgct ggcggccaaa
     2281 atgtttggga tcgcccccca ccatatcctg accggcaacg gcgcctccga gctcatccgg
     2341 gcgatgttcc ggggagaatc cggagccgtc ggcttgtcgg ttccgacctt cgacgaatac
     2401 cgggcctcgg ccggcccgga aagaatcaag ccctttcggc cggtccggac cgatttccgc
     2461 tacacggccg gcgaactggg cgatttctgt ctccgggaga aggtctcggc cctgatcctc
     2521 atcaatcccg acaatccgtc cggcaacttc atctcccgcg gcgaggtcct ccggctggcc
     2581 gaacgcctgg cggaaaagaa catccgcttg atctacgacg aatcgttcct ggatttcgcc
     2641 gacggatcgg ccgatcacag cctgatcgag gggagcgtcc tggagcgcct gccgaatctc
     2701 gccgtcgtga aaagcatcag caaatcctac ggcgtccccg gcctccgctt gggagtgctg
     2761 gcttcctccg accgggccct cctggagcgg gtctggaaag aaatctcgat ctggaacatc
     2821 aactccccgg ccgagcattt ccttcaaatc atcgacaagt acgggaccga cttccgggcc
     2881 gcctgccggc ggatcgccga ggagcgggag cgcttcgtcc gggctcttgg ggagattcct
     2941 tttctccggg tgcttccctc ggccgccaat tacgtccttt gcgaaatcct gccgccgtgg
     3001 acgccggccg ccttgaccga gatcctgctc aatgagcacg acatcctgat taaggacgcc
     3061 tcgggcaagc tcggattcga cgggcggcct ttcgtccggc tgaccgtccg cgacgaaacg
     3121 gacaacgatc ggctcctcgc ggccttgaaa gccctcatcc ggtaagacat gggcatgctc
     3181 cgcgcctggt ttctggaata tttccggtcg ctgaagcgtc ccgatgtgga ggaatgggcc
     3241 gatctctttc tcttccggcc cctggccttt ctcctggtca aggcgatcta ccggacgagc
     3301 ttgactccga accagctttc ggtcttttcg atggttctcg gcgtggccgc cggaattctc
     3361 ttgggaacag gcgggccggc cgcggccgtc gtcgtcgtcg tcctgctgtt tctttccgtc
     3421 gtcgtcgatt gcgccgacgg ccagctagcc cggctgaaga aaaacggcac ccacatgggc
     3481 cgggtcgtcg acggggcgat cgattacgtc gtcggggcgg ccatgtatct cggtctcggg
     3541 ttcggcttcg cctccgcctt ggataacccg gtccggggct ggctgctggt cgcggccgcg
     3601 gccgccagca atatcatcca ttcggtcgtc ttcgactatt accggaaccg ttacttggcc
     3661 tacgtccacg gcgtaagctc cgcccagacc gacgatcgga acgccttccg ccgggaatat
     3721 gtcgagctgg gccgccgggg cgggcaggag ttgcgccgga agttcatccg gctgtatctc
     3781 gcctacatcg ccttccagat gaaaatcagc cgcgatccga tgaccctcgg cccggtggaa
     3841 cccgagatct tccgcaagac gaaccgcctg gcgatgcggc tttggacgtt tctcggatct
     3901 tcgacccaag cggtcctgtt gatgatcgcc cttctcggcg gccggctcct cctgtattat
     3961 tgggcgatga tcgtcgtcct gaatgggatg atgttcgccc ttttcctggg ccagtcggcc
     4021 gtggatctga aggtcaggtc cctcgcggcc gaagcgtcaa agtaaagcgg ccagctcccg
     4081 ggcggcgttc accgaggctc gtttctctcc cagctccgcc cggatgcggg cgaacgcgtc
     4141 cttcatttcc agccgcgcgc ccgaatcgtc caggagccga agcgcttccc ggacgagcgc
     4201 gtccggcgtc atccgccttt ggatcagctc cgggacgatc cgccgcccgg cgaggatgtt
     4261 gacgatgctg tagtcccgga tccggacgaa cggccgcccg gcggcgtagg tcagcgggga
     4321 gatcttgtag aaggcgacga gcggagtccc gagcagggcg gcttcgagat tggccgtccc
     4381 gcaggcggag aggacgagat cgctggcggc catggcctcg taggcgtcct cccgcaggag
     4441 gaggatatcg gctttcgagg cgaccgagag ccgcggcagc agcgccggat cgaggtccgg
     4501 attctggatg aggacgagct gggcccggcg gacggactgc agccggacgg ccgcctcgag
     4561 gacgacggcc aggtggcggc ggatctcgtt ttcccggctt ccgggcatca aggcgatgag
     4621 gggccgggcc ggatccagcc cgcgggcccg gaaaaattcg tcccggctgc tccggatccg
     4681 aagacgctcg agcaggggat gcccgatgta acgatggggg atgcccgccc gggcgtagat
     4741 tctctcttcg aaggggaaga tgaggagcat tttgcggacg acgcttttga tcgtcttcag
     4801 gcggcttttt ctccaggccc aaacggtcgg gctgacataa taaagaacgg ggacgccggc
     4861 cttaaccagc cgccgggcca ggcggaggtt gaaatcgggg gaatcgatca agacggccgc
     4921 ggccggacgg cgggccgagg cctcggtcgc cagccgccgg aggatcttcc ggatgcgggg
     4981 aacgtccttg acgacttcgg ccaggcccat caccgccagg cggtccatcg ggacgaggat
     5041 atccaccccg gcgtcggcca tgttcgatcc cccgacgccg aagaactcgg cccggggata
     5101 gagcaaccgg aattcgcgga cgagacccgc gccgtaattt tccccggatt tctctccggc
     5161 cacgatgagg atcgccggat cactcatttc aacggggtcc cgaaccggat gatttccggg
     5221 ttttgtccgg gagtccggcc gaagaagaac agccaggtgt cgtaggattc gttcttgtcg
     5281 tagatgaaaa acgatttcct cccggagggg gagacgaccc cgatgattct cggattgtcg
     5341 accgacgaaa ggagcttttc cgggacgacc atcacctggg tcaaggagga aggttcccct
     5401 tcggccgctg cgaagtcccc ggcgtcttca ggttcctccc ctttcttggc cggccggctc
     5461 ctcgaagctt tcccccgggc ggccggggct ttcggcccgg gcgccccgag ctcgccggct
     5521 tgggccggca ggaggatgag gttccatttt ccttcccggg tcatgggctc gggaaaggcc
     5581 ttgcgcaagt accggccctt gaccagctcc tcgatcgact tgggatattg cccgggattc
     5641 ttgtccgcgt agagacggac ggcctcgacg tattgacggc cgcggaacag cagctcctct
     5701 tccttctccc tccagccctg tgtttccagg accggcaggg cgatcgccag ggcgaccgtc
     5761 atgacggaga tgaagaccat taaaatgaga agcgtgtagc cggcttccga aggcctcacc
     5821 atgtgttgta gatcgatccg tcgagggctt gggcttccga gcttgatcgg acgtcgacga
     5881 tcccgaggat cttccccggc tcaagctcct cggcggacgg ctcctccatg acttccaccc
     5941 aatccgtccg ggtggtgagg ggatcggccg ggattttccg gagatagcct tcatcgacca
     6001 gggcttggag cgaggcgggg tattttccct tgtcggagaa atactggtcg atcaacttgc
     6061 ggaactggaa gaggtcctcc ttcaaggccg cttccttggc tttcttgacg gcgttgcggt
     6121 attcggggag ggccaggccg acgaggatgc cgatcatggt gaagacgatg atgatttcga
     6181 tcagcgtata gccgcgttcg tgttttcggg ccacgactcc tcctccttca ccagtcggcg
     6241 tatttcgagc cgtcgagggc cgtgcgccgg ctcttggtgt agatgtcgta gacgttttcc
     6301 ccgccccagg ttgtcgcatc gggcggatcc tgattggagc gctggcccca ttccgacgtc
     6361 ccggtcatcg ggtcgacggg aatccggcgc agaaacttga agatcttttc cttcccgctt
     6421 ccaagcttcc ggccgttttc ccctcggtct ccttcatccg tcgacgcggc cagaggcact
     6481 cctttgacca ggacgtccag cgtcggggga tagttgtcgg cttcgccgcc ttcctcgacc
     6541 tcgatcgtgc cctcatcggc catccttttg taggagtcga tggcctcccg gacgagacgg
     6601 agggcgcggc ggagctcgac ttccttgtcc cgctggacgg tcaccttgac gaccggcacg
     6661 gccacgccgg ccaacacggc caggatcgcg gccgtgacca gcatctccgt caaggtgaat
     6721 cccggccgca ggcgcatgat cctcctcccg ccggcccgcc gcccgtaaga ccgggggggc
     6781 cgccggccgc tcagtaccgt ccccgtccgc cgccttttcc gccgcggccg cggtcgcggt
     6841 cggagcgggg cgggcgggga cgggagtccg gatggcccga gccgcccttg tccccggttc
     6901 cctcaggcgg gacggcgtcg ggattgaggg ctttcatgct caggcggatc cggttgtctt
     6961 caatcgtcag gaccttgacc gtgatgacgt ccccgagatg aaggacgtcg ccgacgctcc
     7021 ggatgtggta gggggcgatt tcggagacgt gcattaaacc gacgatgtta ggcatgatct
     7081 cgacgaacgc gccgtactcc tcgatccgga cgactttgcc ggtgtaggtc tggccgactt
     7141 cggcctcggc ggtcaggtcg aggatcatct gcttggcccg ttcggccgct tccatgtcga
     7201 ccgaagcgat gaccaccttg cccgtctcgt cgatgtcgat tttggccttg gtcgtggaga
     7261 cgatcttctt gatgatcttc ccggcggggc cgatgacctc gccgactttc tccggattga
     7321 tgtagaggaa gaggatccgg ggcgcgtaag gggagatctc ggtccgcggc cgggggatcg
     7381 cttccttcat tttgtccagg accttgagcc gggcggcccg ggctttcatc atcgcctcgc
     7441 ggcagacggc caaggggatg gcgtcgatct tgaggtccac ttggatggcc gtgatgcctt
     7501 tttccgagcc ggcgaccttg aaatccatgt cgccgaagtg gtcctcgatc ccggcgatat
     7561 cggtcaggac gacgaaccgg tctccgtcct tgaccaggcc catggcgatg cccgcgggaa
     7621 cgaacgtcag cggggcgccg gcgtccatca gggcgagcac gccgccgcag accgtggcca
     7681 tggaggagga gccgttggat tccaggatgt ccgagacgat ccggacggtg taggggaact
     7741 gctcctcggc cggaatggcc gggaggatgg atgtctcggc cacggcgccc tggccgagca
     7801 atccatcctc ccggccattc cggccgagga gcagttc
//
LOCUS       DAOOXH010000103         7649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_10785_length_7649_cov_7.441796, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000103 DAOOXH010000000
VERSION     DAOOXH010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10785_length_7649_cov_7.441796"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="PKV64_10000"
     CDS             complement(<1..1089)
                     /locus_tag="PKV64_10000"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPN17444.1"
                     /translation="MDLDSLFLKRTPPHSPEAERAVLGGILINSRNYSLVLSTVAIED
                     FYKDAHRKILETVGRLVDRGVPVELLAVSEELRREGRLEDVGGAAYLSSLMDGVPRSS
                     NVEYYARIIKEKSLLRRLILSSAQIIAASYEEKEDADDLVNAAQAAMIDVAEQRDRRG
                     FVPLSQVIGPTMEHVQKLVATRDSVTGVDTGFYELNKLTAGFHPSELIIVAARPSMGK
                     TAFCLNMTQYIGLRAGKAVGFFSMEMSQESLLIRMLCADARIDLQHVRSGLISDRDLD
                     RLRLSAETLGPCPIFLDETPALTLAEMKAKARRLKLEGLLDIVFIDYIQLMRAGGRFE
                     NRNQEMSFISRSLKELAKELAIPVVGISQ"
     gene            complement(1127..1612)
                     /gene="rplI"
                     /locus_tag="PKV64_10005"
     CDS             complement(1127..1612)
                     /gene="rplI"
                     /locus_tag="PKV64_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPN17445.1"
                     /translation="MKVILKQDVENLGRKGDVVNVASGYGRNYLIPRKLALEVTASNL
                     KMIEIEGAALKKKAEQERASFQETIQKLNQVTLSFTRKTTEKDHIFGSVTAADIKEAL
                     DAQGFAVDKKRIVLPEPIKQLGSHKVTIKVYHDDKAEIQVDVVAEEEPPAETEAPPAE
                     A"
     gene            complement(1626..1901)
                     /gene="rpsR"
                     /locus_tag="PKV64_10010"
     CDS             complement(1626..1901)
                     /gene="rpsR"
                     /locus_tag="PKV64_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPN17446.1"
                     /translation="MAEEKRFERSDRPEKPAYRKFFPPKKKFCRFCQRNVKFIDYKNA
                     DILKKYIPDRGRISPRRVTGTCAFHQRKLAAAVKRARHMALLPYVTE"
     gene            complement(1904..2293)
                     /gene="rpsF"
                     /locus_tag="PKV64_10015"
     CDS             complement(1904..2293)
                     /gene="rpsF"
                     /locus_tag="PKV64_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPN17447.1"
                     /translation="MKQYETGFLLAPNMTEEEAEGVVQGLAEVVPLKNGKMIRIEKWG
                     KRRTAYPIGKAHEAYYVFFHYEGGAEIPLELSRRFRQMDTVIRHLTLVKETRMNVRKK
                     KKVRKNAPSAADAETAAPAAEPKREEA"
     gene            complement(2374..2928)
                     /gene="pth"
                     /locus_tag="PKV64_10020"
     CDS             complement(2374..2928)
                     /gene="pth"
                     /locus_tag="PKV64_10020"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPN17448.1"
                     /translation="MWAVVGLGNPGLRYADTRHNIGYMLVKKAAKAWEIRLRKPKFSA
                     RTGEGRRAGERIVLALPETYMNESGQAVKALLVGMEIPTERLVVVFDDVDLPLGEIRV
                     RREGGPGTHRGMASIARLIGTSFPRIRVGIGPVPENVDITDFVLEPFRKAEKKLLDGS
                     LDRAMDALELVLDGRIDQAMNSYN"
     gene            complement(2932..3603)
                     /locus_tag="PKV64_10025"
     CDS             complement(2932..3603)
                     /locus_tag="PKV64_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPN17449.1"
                     /translation="MNLTIPTQPREAFGKNASYRIRQTGRIPAVLYGEGRPSQALVVD
                     KKDIIRILKSETGLNTLFKITLEDGERDVMIKDVQINPLTDEVIHADLIQIDMNKAIR
                     VEVPIELVGEAVGVKAEGGFIDFMTRALMIECLPAMIPEHLIVDVSGLRLHQSIKVSE
                     VVLPEGVKLITEPGAVVALVQVPHEEKAEAAPAVEEEVEAAAAAPAEPEVIKKERAEK
                     PEPEK"
     gene            complement(3618..4544)
                     /locus_tag="PKV64_10030"
     CDS             complement(3618..4544)
                     /locus_tag="PKV64_10030"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPN17450.1"
                     /translation="MKLFAGSSNPGLAREIAAYLRIDLGTCVLDRFSDGEIHFYIDEN
                     VRGEDIFILQSGSNEANFNLMELFIMIDAFKRASADRITAVIPYYGYARQDWKDRPRV
                     PISARLVADLLETAGADRILTMDLHSPQIQGFFSVPVDNLTVVPVMVKHVQSRGLKNI
                     TIVSPDAGGVGRAKHFARRLDAPLAIIDKRRPAPNVAQVFNVIGDVVGQTCVLFDDMV
                     DTAGTLCLSAEALKNAGAAEVLACSTHAVLSGKALDKIESSPIETLIVSNTIAPEKRR
                     ACAKIETLSVAELFGEAIRRINEKSSVSSLFD"
     gene            complement(4591..4662)
                     /locus_tag="PKV64_10035"
     tRNA            complement(4591..4662)
                     /locus_tag="PKV64_10035"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4628..4630),aa:Gln,seq:ttg)
     gene            complement(5519..6199)
                     /locus_tag="PKV64_10040"
     CDS             complement(5519..6199)
                     /locus_tag="PKV64_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17451.1"
                     /translation="MKAPASFPSGFRFLLGTALVVWGLAAAACRPSRPLLRPGEDLRE
                     LEGYASLRLTRGEESSRSKFAFAVLLPHRARLDIFDALGRAVSIFLIRGEDAYLALPS
                     ERIYWRGGRDEVIEKFLGFPVRPLEMAGLISGRWSEPSQEGWKLVRDARGRVVSGERG
                     GLSFRVLEFSSGGSDVPRRWTFRHAGTEGTVTLLEAAFDRSEPDFSLDFLRTCRSVTW
                     PEIEKLFR"
     gene            complement(6216..6533)
                     /locus_tag="PKV64_10045"
     CDS             complement(6216..6533)
                     /locus_tag="PKV64_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17452.1"
                     /translation="MNIGMIGIFVIFGIFIILLAANPRLSCFGRKLRSPLYPLYRKKK
                     MEEEARRLRQAKLRTLKTEDYGFKLDDSPPAADAGAPSRPSVSEGAGKNAASAEDYGL
                     KLD"
     gene            6886..7164
                     /locus_tag="PKV64_10050"
     CDS             6886..7164
                     /locus_tag="PKV64_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPN17453.1"
                     /translation="MPIYEYACEKCRQRIERIQKVSDPPCQKCPHCGGPLRKLISSPA
                     IQFKGSGFYINDYAKKGASPAEGKKKTGRKEKAEKAAESVAKPVAGAE"
     gene            7184..>7649
                     /locus_tag="PKV64_10055"
     CDS             7184..>7649
                     /locus_tag="PKV64_10055"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPN17454.1"
                     /translation="MKIVVRTPNWLGDALMAFPALTALRRHFPDDEISIIAPDGIGDL
                     YSGPETEARSFHRGPSARGSSLRRAAAELRNERFEAGLLLTNSFASALVFALARIPER
                     WGYARDGRGPLLTRRVRIRKSDEPVHMVRYYTDLMEGLGIPVPPPDIRLSVSE"
BASE COUNT         1433 a         2424 c         2335 g         1457 t
ORIGIN      
        1 ctgggagatg ccgacgacgg ggatggccag ctccttggcc aattccttga gcgagcggga
       61 aatgaacgac atttcctggt tgcgattttc aaaacgcccc ccggcccgca tcagctggat
      121 gtagtcgatg aagacgatgt ccagaaggcc ctccagcttc agccgccggg ccttggcctt
      181 catctccgcc agggtgaggg ccggcgtctc gtcgagaaaa atcgggcagg ggccgagcgt
      241 ttccgcgctc aagcggagac ggtccaggtc ccggtcgctg atcaagccgg accggacatg
      301 ctgcagatcg atcctcgcgt cggcgcagag catccggatg aggagcgact cctgggacat
      361 ctccatcgag aaaaagccca cggctttccc cgcccgaagg ccgatatact gagtcatgtt
      421 caggcagaag gcggtcttgc ccatggaggg gcgggcggcg acgatgatga gctcggacgg
      481 gtgaaacccg gccgtgagct tgttgagctc gtagaagccc gtgtccactc cggtgaccga
      541 gtctcgggtc gccacgagct tctggacgtg ctccatcgtc ggtccgatca cctggctcaa
      601 ggggacgaag ccccggcggt cccgctgttc ggccacgtcg atcatcgccg cctgggcggc
      661 gttgaccaga tcgtcggcgt cctccttttc ctcgtagctg gcggcgatga tctgggcgga
      721 ggaaaggatg agccgccgga gcaggctttt ttccttgatg atcctggcgt agtattcgac
      781 gttggagctg cggggcacgc cgtccatcaa agaggacagg taggccgcgc cgcccacatc
      841 ctccagccgc ccctcccggc gaagctcctc gctgaccgcc aaaagctcca ccggcactcc
      901 gcggtccacg agccggccga ccgtctccag gattttccga tgggcgtcct tgtagaaatc
      961 ctcgatggcc acggtcgaga gaacgaggct gtaattgcgg gaattgatca ggatcccgcc
     1021 caggaccgcc cgttccgcct ctgggctgtg gggcggcgtt cgtttcagaa acagcgaatc
     1081 cagatccacc gctcctcctt tcgggccttc cggcccgccg gcggcctcag gcttccgccg
     1141 ggggagcctc cgtttccgcc ggcggctctt cctcggcgac gacgtccact tggatctcgg
     1201 ccttgtcgtc gtgatagacc ttgatggtca ccttgtggga acccaactgt ttgatcggct
     1261 cggggaggac gatccgcttc ttatcgacgg cgaagccctg cgcgtccaac gcctccttga
     1321 tgtcggcggc cgtgaccgag ccgaagatgt gatccttctc cgtggtcttg cgggtgaagg
     1381 acagggtcac ttggttgagc ttctgaatcg tctcctggaa ggaggcccgc tcctgttcgg
     1441 cttttttctt caaggccgcc ccttcgattt cgatcatctt gaggttggaa gccgtcactt
     1501 cgagggcgag cttgcggggg atgaggtaat ttcggccgta gccggacgcg acgttgacga
     1561 cgtctccctt ccggcccaag ttttccacgt cttgtttcag gatgactttc atgggacctc
     1621 cggtcttact cggtgacgta gggcagaagg gccatgtgcc tggcccgctt caccgccgcc
     1681 gccagtttgc gctggtgaaa agcgcacgtg ccggtcaccc ggcggggcga aatccggccc
     1741 cggtcgggga tgtacttttt caggatgtcg gcgtttttat aatcgatgaa cttgacgttc
     1801 cgctggcaga accggcagaa cttctttttc ggaggaaaga atttcctgta cgccggcttc
     1861 tccggccggt ctgagcgttc gaaccgtttt tcttcggcca tgatcaagcc tcctcgcgct
     1921 tgggctcggc ggccggggcc gccgtctccg cgtccgccgc ggacggggcg ttcttccgga
     1981 cttttttctt cttccggacg ttcatccgcg tttctttgac cagcgtcagg tggcggatga
     2041 ccgtgtccat ctggcggaac cgccggctga gctcgagcgg gatctccgct ccgccctcgt
     2101 aatggaagaa cacataatac gcctcgtggg ccttcccgat gggataggcc gtccggcgtt
     2161 tgccccactt ttcgatccgg atcatcttcc cgtttttcag gggaacgacc tcggccaggc
     2221 cctggacgac gccctcagcc tcctcctcgg tcatattcgg ggcgaggaga aaccccgtct
     2281 catactgctt catccaaacc tccttttggt ttgacagcat ccccctcttc ggcgggggga
     2341 gcaaggagga agttttcgct tcctcaacga acgctaatta tagctgttca tcgcttgatc
     2401 gatccggccg tccaggacaa gctccaaggc gtccatggcc cggtcgaggc ttccgtccaa
     2461 aagcttcttt tccgctttcc ggaacggctc caggacgaaa tccgtgatgt cgacgttctc
     2521 gggcacgggc ccgatgccga cgcggatccg cgggaacgac gtcccaatca gcctggcgat
     2581 cgaggccatc ccccggtggg tgccggggcc gccctcgcgg cggacacgga tctcgccaag
     2641 cggcaggtcg acgtcgtcga agacgaccac caggcgctcc gtcgggatct ccatcccgac
     2701 gaggagcgcc ttgacggcct ggccgctctc gttcatatac gtctccggca gggccaggac
     2761 gatccgttcg cccgcccgcc gtccttcgcc cgtcctggct gaaaacttgg gttttctcaa
     2821 acggatttcc caagccttgg ccgccttctt caccaacatg tacccgatgt tgtgacgcgt
     2881 gtcggcatac cgcaagcccg gattccccag gcccacgacc gcccacacgc tctatttctc
     2941 cggctcgggc ttttcggccc gctccttctt gatgacttcg ggctcggccg gcgccgccgc
     3001 cgccgcttcc acctcttcct cgaccgccgg cgccgcttcg gccttctcct cgtgcgggac
     3061 ctggaccaag gcgacgaccg cgcctggttc cgtgatcagc ttgacgccct cgggaaggac
     3121 gacttcggag accttgatcg actggtgaag gcggagtccc gagacatcga cgatcaggtg
     3181 ttcggggatc atcgccggga ggcactcgat catcaaggcg cgggtcatga agtcgatgaa
     3241 tccgccctcg gccttgacgc cgacggcttc gccgacgagc tcgatcggaa cctcgacccg
     3301 gatcgccttg ttcatgtcga tctggatcaa atcggcgtga atcacctcgt ccgtcaaggg
     3361 attgatctgg acgtccttga tcatgacgtc gcgctctccg tcttcgagcg taatcttgaa
     3421 gagggtgttc agaccggttt cagacttgag aatccggatg atgtccttct tatccacgac
     3481 aagggcctgg ctgggccggc cttcgccgta aagaacggct ggaatccggc cggtctgtcg
     3541 gatgcggtag gacgcgttct tgccgaacgc ctcgcggggc tgggtgggga tcgtcaggtt
     3601 catggaagtc gtcctcctta atcgaacagc gagctcacgg agcttttttc attgatccgg
     3661 cggatagcct cgccgaagag ctcagccacg gacagcgtct cgatcttggc acaagcccga
     3721 cgcttctccg gagcgatggt attagacacg atgagcgttt caatgggcga tgactcgatt
     3781 ttgtccaggg ccttgccgga aaggacggca tgggtggagc aggccaaaac ctcggccgcc
     3841 ccggcgttct tcagggcctc ggcgctcaga cacagcgtcc cggcggtatc gaccatgtcg
     3901 tcgaaaagga cgcaggtttg gccgacgacg tcgccgatga cgttgaaaac ctgggcgaca
     3961 ttgggggccg ggcggcgctt gtcgatgatg gccagcgggg cgtccaggcg ccgggcgaaa
     4021 tgcttggccc gcccgacccc gccggcgtcg ggcgagacga tggtgatatt cttcaggccg
     4081 cggctttgga catgcttgac catgaccggg accacggtga ggttgtccac gggaacggag
     4141 aagaatccct ggatctgggg agaatgcaga tccatggtca gaatccggtc ggcccccgcg
     4201 gtctccagga gatcggcgac caggcgggct gaaatcggaa cgcggggccg gtccttccaa
     4261 tcctggcggg cgtacccgta atacggaatg accgccgtga tccggtcggc cgaggcccgc
     4321 ttgaaggcat cgatcatgat aaagagctcc atgaggttga aattggcctc gttggagccg
     4381 ctctgaagga tgaagatatc ctcgccccgg acgttttcgt cgatgtagaa atggatttcc
     4441 ccgtcggaga agcgatccag gacgcaagtg cccagatcga tccgcaggta ggcggcgatc
     4501 tcccgggcca agccgggatt cgatgaaccg gcgaacagct tcatggtcgt atccttttat
     4561 gtcgcgcgcc cggcgcgttt atttccggag ctggggcggg aggattcgaa cccccgaatc
     4621 gcgggtccaa agcccgctgc cttaccgctt ggcgacgccc cagacgtgat tgcgtcccat
     4681 ccttgggccc ggctcagcgt cgacgccgtc acggcgtccc aacggccggc caacgcggcc
     4741 cggcatcttt cggcctcctc gcgtttctcg aaaaggccga aaacacccgc cccggaaccg
     4801 gtgacgagag ataaaaccgc gccctgatcc cgcaaaaagc gtttaatctc gttcaactga
     4861 gggaagaagc gaaaaatcgt ctcttcgagc gcattcgtca gggagccgag ttcccgggtc
     4921 ccgagaaact gcgtaatttt actggcttta tcggcggaag tcaaggaggc tggaggcaag
     4981 gactcgtaaa tcaggcgcgt cgacacggtc aagggaggaa gaacgagaac gagccaacgg
     5041 accggcagat cgggcagggg ggtcagccgg tctccccgct cctcccccag gcatagaccg
     5101 ccggtgaaaa aataggggac gtccattccc aacttccgcc ccaaggcttc gagatcggcg
     5161 ggttctagcc ccaactccca gagccggttg agtcccagga gaacagcggc cgcgttcgag
     5221 cttccgccgc cgaatcctcc gccggccgga accctcttgg ccacgacaat ttccgccccg
     5281 cggcggcttc cgccggcctc ccgaaggaga acggccgcct tgaaaacgag attggactcg
     5341 tcccagggaa ttgaaggatc gtcccctcga agccggatct ccggcccgtc gagaggccgc
     5401 aactccaaga ggtcgaagaa atcaacccac tgaaacagcg tccggatgtc atggtagccg
     5461 tccgggcgct ttcccaaaat ttccagcccg aggttgacct tggcgaacga aggaatcctc
     5521 accggaagag cttctcgatt tccggccagg tcacggaacg gcaggtccgg agaaaatcca
     5581 aggagaaatc cggttcggac cggtcaaagg ccgcctccag cagggtgacg gtcccttccg
     5641 tcccggcgtg gcggaacgtc cagcgccgcg gcacgtcgct tcccccggag gagaattcca
     5701 agacgcggaa cgacagaccg ccgcgctcgc ccgagacgac ccgtccccgg gcatcacgaa
     5761 ccaatttcca gccttcctgg gacggttcgc tccatcgtcc ggaaatcaat ccggccattt
     5821 ccaagggacg gacgggaaat ccgaggaatt tctcgatgac ctcatcccgc ccccctcgcc
     5881 agtatatgcg ctccgagggc agggcgagat aggcgtcttc gccccggatc aggaaaatcg
     5941 aaaccgcccg gccgagggcg tcaaagatat ccagccgggc ccggtgcgga agaagaacgg
     6001 cgaaggcgaa tttcgagcgg gatgactctt ccccgcgggt caggcggagc gaggcgtatc
     6061 cctcaagctc ccgcaagtcc tcgcccggtc ggaggagggg gcgggagggg cgacaggccg
     6121 cggccgccaa gccccagacg accagcgcgg ttcccagaag gaaacgaaat ccggaaggga
     6181 aggaggcggg ggccttcacg atttctcgtc tatcttcaat ccagcttgag cccgtagtcc
     6241 tcggccgagg cggcgttctt tccggccccc tccgaaacgg acgggcggga aggcgccccg
     6301 gcatccgcgg ccggcgggga atcgtcgagc ttgaatccgt aatcctccgt tttcaaggtc
     6361 cgcagcttcg cttggcgcag acgccgggcc tcctcctcca tcttcttctt ccgataaagg
     6421 ggatagagcg gcgacctgag ctttctcccg aaacaggaga ggcggggatt ggccgcaagc
     6481 agaatgatga agatgccgaa aatgacgaag atgccgatca tgccgatgtt cattgccgcc
     6541 tctttccagc cagcccccgg tcccgatcgg gccggccggg cgaaaaaaag gaacggcccg
     6601 cgccgccgga cgggcggaac gcccccgttc ccgaagaatc agttcttctg gaattcggga
     6661 attttgcgcc gctctttatc cttgcccagg ttgaacccga ggaaaaatac cgagaaaaga
     6721 acgacgatcc cgagcaatgt cttaaaaaag ccgttcataa caacatacct tccgcaaaaa
     6781 agcttcgctc ttattttacc caaggggaac gaattgtcaa attttcccat ccgctcgaac
     6841 tttgctataa tgaacgggtt aaatctcatc gcgaaggatg cgccgatgcc gatttatgaa
     6901 tatgcctgcg agaaatgccg gcagcggatc gaacggatcc agaaggtttc cgatcccccc
     6961 tgccaaaaat gccctcactg cggagggcct ctccggaaac tgatctcctc tccagccatt
     7021 cagttcaagg gatccggatt ctacatcaac gactacgcta aaaagggcgc ctccccggcc
     7081 gagggaaaga aaaagaccgg gcggaaggaa aaggccgaaa aagcggccga atccgtcgcc
     7141 aagccggtcg ccggcgcgga gtaagaggcg gcaatcccgg cccatgaaaa tcgtcgtccg
     7201 gacgccgaat tggctcggcg atgcgctgat ggccttcccc gccctgaccg ccctgcgccg
     7261 gcattttccg gacgatgaga tttcgatcat cgccccggat ggaatcggcg atctctattc
     7321 cggaccggaa acggaggcgc gctcgttcca ccggggtccg tcggcccggg gatccagcct
     7381 ccggcgggcg gccgccgagt tgaggaacga gcgattcgag gccgggcttc ttttgacaaa
     7441 ttccttcgcc tcggccttgg tgttcgccct cgcccggatt cccgagcgct ggggatacgc
     7501 gcgcgacggc cgcggccctc ttttaacccg gcgggtccgg atccggaaaa gcgacgagcc
     7561 cgtccacatg gtccggtact acacggacct gatggagggg ctgggaatcc cggtccctcc
     7621 tcccgacatc cgcctctccg tgtcggagg
//
LOCUS       DAOOXH010000104         7498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_11104_length_7498_cov_5.651619, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000104 DAOOXH010000000
VERSION     DAOOXH010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11104_length_7498_cov_5.651619"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PKV64_10060"
     CDS             <1..449
                     /locus_tag="PKV64_10060"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN17455.1"
                     /translation="YLNNLGTTFMKIGNNEKAVETFKKMLEIQPESYSANKNLGSLFG
                     EAKDYQGALPYYAKASELSPNDPDAFYNLGACYLNLQEYDKAEAAFLKVKELTPDYAP
                     AYYQLGMIYVNQNKKPEAIENLERYIELAPDEPNADIAKRILAYLK"
     gene            549..2309
                     /locus_tag="PKV64_10065"
     CDS             549..2309
                     /locus_tag="PKV64_10065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPN17456.1"
                     /translation="MMKDTRGRRLPAMLAVLAFGLTGAAFGQDPSAQVPKAPEKKKEE
                     PADKMPERYEKWLNEEVLYIISQNERDVFKSLKTDEHREGFIKTFWKRRDPTPDTPVN
                     EFREEHYRRIAYANKTYFEGKAGWRTDRGRVYIMFGPPDFFETNPGGGRGFLLGVNAP
                     TAEFPAEVWTYREIPGLKERIGRVDFTFVNYYGAGSWQLTSNPALANALRNVSLPARY
                     AGYGDMDLKDGHAYSATTAAQVGQGQFMQNAIEQLQVMAELTKSRGEVLEELERSERL
                     RRLRGIVESKPSLAGLSFHARESYLAGGDGMTLVPISLEIAAKDVAFRPIEDRYRGMV
                     NFYIEVKDDKGTAFQASDRLEMNLKEESYRRRYADSYQYKQSLSLKPGKYFLHMVVWD
                     EYNSNVGYVDRWIDVPAFPAENLSLSDIILARAIRRVENKTQDVPMNLQDIPALKALE
                     KTNLKVPDKLSLSGQKSGPFLFGNLEINPNTLAAVAPGGDLIFFYQIYNPTYDEESGT
                     ARIRIENQIWQGTKLVASIDDIPELKVPIAQKAAGPLNSSGKYSLGSLPPGSYVLLAR
                     VGDLFSGKTAEKRIAFKIEK"
     assembly_gap    2693..2792
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2835..6038
                     /locus_tag="PKV64_10070"
     CDS             2835..6038
                     /locus_tag="PKV64_10070"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPN17457.1"
                     /translation="MNLRKLTILFAAFLMVTSLGLAQSKDYGTFIGAVYSTEGTPLPG
                     VSITAKNLDMGLSQSTITNDQGFYRIERLPRGKYTLTAALPGFKTLTKEGFELSIGAE
                     VKINFDLEIGDIEEEVTVIGVTPLVETTRAQVSTVITEKEFLSYPQGNRDYTSLIAYA
                     PGTLPGAGRSGYAINGMRGSSNNFQIDGIDNNDNGTASGSGNITSMPPEAIQEFRLVS
                     NNFSAEYGRNSGGVINAVMKSGTNELHGSAWVFHKGTGGFFSSEDWLTHDYIEQDRTQ
                     YGATIGGPIIKDKTFFFATFEGVMENYGSATPYYFFTPDAIKRAGPTAKVFFDKVGSN
                     YPVPTYDFIDVDGDGSIDAGRYIWDGTGKYRGYTGGLKIDHIFSEKDRIAVRWLFNTY
                     TDKWDFANVPGNIKEVPYAYHTGGLTWLHLFSPTMYNELRLGYHRDYADWKRVAPEIP
                     QLGGYTYFNDGVHAIGDWANMPQEFTNNTYQLVDVLNFQAGNHSIKVGGELRLWNSDS
                     MFDANVAGAYYFFDSTWFLYDLGAYYLYIGADPPDPPKDNPYVKGDPWGEWKQGDSRR
                     KWQGLEGGVFVQDDWRVTERFTLSLGLRWEYYGVPEEKSGKGINMPAFGTKAGFESKQ
                     VIEGVEGEEGVRYLIFDGRQLLGKGLWNPYYLAFAPKVSFAWDVTGDGKTSLRGGFGI
                     SYDRTFNNTYENDRFNYPDFTFVGLLGILDGSPAIDVAYPAVVPMANVAGYGASLRWM
                     LPDLKPQKAYNWLFGIQRELSSNVSIEINYTGSHGANLGSIQRPNRFTGDKLDETRTG
                     INPYCAITGVNLRTQNLESDYNALTVTLNKRFSDGWSWYTAYTFGYAYDQNSDYFGDN
                     TSMEAVSNERIDMERGFAQFDRRHRVVGGFVYDIPWFKNSDNWLLKNVIAGWQIAGNF
                     DVTSGAPFTIRGASSSTDWNYDWDNNDRPLWLGDDDTDIITWTNGRPGWDKSNFGIPV
                     KPAGPNDLSYYDQNFAERNQFRWFPTHNINIALQKYWVLNVGGKEFTIQGIFEVFNLL
                     RSYFWALPSTSWTAADFGVSTRMNGRRTSQISLRIMF"
     gene            6147..>7498
                     /locus_tag="PKV64_10075"
     CDS             6147..>7498
                     /locus_tag="PKV64_10075"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HPN17458.1"
                     /translation="MNIKRFSERPAGRLRLHKGLVFLGVLLWAGAGLRLFGAPQGKVR
                     RDAGLNILLVTLDTTRADRIGAYGWAKAKTPVIDTLAAGGVRFANAYCPAPLTLPSHA
                     SILTGTYPLYHKVRNNGSYYLGPEAVTLAERLKEKGYRTSAVVASFNVDSRFGLDQGF
                     DFYDDKFSDDEMLKTFRSERRADEVADAFLSWFEGHAQEKFFSWIHFYDPHMPHDVPS
                     PYKEEFAGDLYDGEIAYMDHHFGRIIDRLRETKVLDKTLIVIAGDHGEALGDKGELDH
                     GIFIYDVTMKVPLIFYGPKVLPAGRVSASRVRLIDVMPSILEMAGIPANKEIQGASLL
                     PFIEGKRKDDLSCYLESYYPLETYGWSQLLGFIDGDWKYIQAPRSELYNLKQDAREET
                     NLFPQESKTAAALDKKLLAAIRDYSSKAEAGRRKLSREEEERLRSLGYVGSDASVKIP
                     "
BASE COUNT         1677 a         2300 c         2098 g         1323 t
ORIGIN      
        1 cctatctcaa caacctcgga acgaccttca tgaagatcgg gaacaacgag aaagccgtcg
       61 agaccttcaa gaaaatgctg gaaatccagc cggaaagcta cagcgccaat aaaaatctcg
      121 gctcgctctt cggcgaagcc aaggactatc agggcgccct gccctattac gccaaggcct
      181 cggagctctc cccgaacgac ccggacgctt tctacaatct cggggcgtgt tacttgaacc
      241 tccaggaata cgacaaagcc gaggccgctt tcctcaaggt gaaggagctg acgccggatt
      301 atgctccggc ctactaccag ctgggaatga tctacgtcaa tcagaataaa aagcccgaag
      361 ccatcgaaaa cctggaacgg tacatcgagc tggctcccga cgagccgaac gcggatatcg
      421 ccaagaggat cctggcctat ttgaaataaa cccggaaccc gggtttcagg cggacgcgcc
      481 ggcggaccga agcggacgtc cctccgccgc aacgaaacgg acgaacgaga agtattggag
      541 gtcggacgat gatgaaggac acccgcggaa gaagactgcc ggcgatgctc gccgtcctgg
      601 cgttcggcct caccggagcg gctttcgggc aggatccgtc ggcccaggtc cccaaggcgc
      661 cggaaaagaa aaaagaagag ccggccgata aaatgccgga acggtatgag aaatggctca
      721 acgaggaagt cctctatatc atctcccaga acgagcgcga cgttttcaaa tccctgaaga
      781 cggacgaaca ccgcgagggc ttcatcaaga cgttctggaa aaggcgcgac ccgacccccg
      841 acacccccgt caatgaattc cgggaggagc attaccggcg gatcgcgtat gccaacaaga
      901 cgtattttga agggaaagcc ggctggcgga ccgaccgggg gagggtctat atcatgttcg
      961 gcccccccga tttcttcgag acgaatcccg gcggcggccg cggatttctg ctgggcgtga
     1021 acgccccgac ggccgaattt ccggccgaag tctggacata ccgggagatc cccgggttga
     1081 aggagcggat cggccgggtg gatttcacct tcgtcaatta ctacggcgcg ggatcctggc
     1141 agctcacctc caatcccgcc ctggccaacg ccctccggaa cgtctccctg cccgcccgct
     1201 acgccggcta cggcgacatg gatttaaagg acgggcatgc ttattcggcg accacggccg
     1261 cccaggtggg ccagggccaa ttcatgcaga acgccatcga gcagctccag gtcatggccg
     1321 agctgaccaa gtcccgggga gaggtgctcg aggaattgga gcggtccgag cggctccggc
     1381 ggctgcgggg gatcgtcgag tccaagccgt ccttggccgg cctctctttt cacgcccggg
     1441 aaagctacct ggcgggcggc gacggaatga ccctcgtccc gatcagcctg gagatcgcgg
     1501 ccaaggacgt ggccttccgc ccgatcgagg accggtaccg gggcatggtc aatttctaca
     1561 tcgaggtcaa ggacgacaag gggacggcct tccaggcgag cgaccgcttg gagatgaatc
     1621 tcaaggaaga aagctaccgg agacgatacg ccgactccta tcaatacaaa cagagcctga
     1681 gcctgaagcc gggcaagtat ttcctccaca tggtcgtttg ggacgagtac aacagcaatg
     1741 tcggatacgt cgatcgctgg atcgacgtcc ccgcgttccc ggcggagaat ctgagcctca
     1801 gcgacatcat cctggcccgg gccatccgga gggtggagaa caagacccag gacgtgccga
     1861 tgaaccttca ggacatcccc gcgctcaagg ccctggagaa gacgaatttg aaagtccccg
     1921 acaagctcag cctgtccggc cagaagagcg ggccgttcct tttcggcaat ctggaaatca
     1981 atcccaacac gctggcggcg gtcgccccgg gcggcgattt gatcttcttc taccagatct
     2041 acaatccgac ttacgacgag gaaagcggaa cggctcgaat ccggatcgaa aatcagatct
     2101 ggcaagggac caagctcgtg gcctcgatcg acgacatccc ggagctgaag gtcccgatcg
     2161 cccaaaaggc ggccggacct ttaaacagca gcggcaaata cagcctggga tccctcccgc
     2221 ccggaagcta tgtgctgctg gcgcgggtcg gggatctttt ttcagggaag acggcggaga
     2281 aacgcatcgc ttttaaaatc gaaaagtaaa ccgcgtccgt ccggtctcca cccggaaaaa
     2341 tccaattaat tgaaatatat ccaaaatatt gaatcgcgct ttttcaggcg ttattcttat
     2401 tttttataat tatatttatt atcaacaatt tatgggcgtt ttttttaacg taaaaaaatg
     2461 gcatggatat tgcattaaaa gaacctgatt gaggaagaga gcatatcgag gaagagagta
     2521 attatgaatg cgaaaatata ttttcaggag gtgataatta aacaggtaag cgcgaacgaa
     2581 aaaacggttg gttgtttccg ccgcggcaat ccatatgcag ccgctgcggg aaggggaaaa
     2641 aaacaaatcc cgagtcatcg ggaccgagga ggaatttgaa tgaatttgag aannnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaaaaaca aatcccgagt catcgggacc
     2821 gaggaggaat ttgaatgaat ttgagaaagc tgacaatcct atttgctgct ttcctcatgg
     2881 tgacctccct cggcttggcg caatccaagg actacggcac gtttatcggc gctgtttaca
     2941 gcaccgaagg cactccgctt cccggagtct ccatcacggc caagaacctg gatatgggct
     3001 taagccagtc gacgatcacc aacgaccagg gcttttaccg gattgagcgc ctgccccggg
     3061 gcaaatacac cctgacggcg gccctgcccg gattcaagac cctgaccaaa gaaggctttg
     3121 agctttcgat cggcgccgag gtgaagatca acttcgacct ggaaatcggc gacatcgaag
     3181 aggaagtgac cgtcatcgga gtcaccccgc tcgtcgagac gacgcgggcc caggtctcca
     3241 cggtcatcac cgagaaagag ttcctgagct atccccaggg caaccgcgac tacacgtcgc
     3301 tgatcgccta tgctccggga acgcttcccg gcgcgggccg gtccggctat gccatcaacg
     3361 gcatgagagg atcgtccaac aacttccaga tcgacggcat cgacaacaac gacaacggaa
     3421 cggccagcgg cagcggcaac atcaccagca tgccgcccga ggcgattcag gagttccgtc
     3481 tggtttcgaa caacttctcg gccgaatacg gccgcaactc cggcggcgtc atcaacgccg
     3541 tcatgaagtc cggaaccaac gagcttcacg gaagcgcctg ggtgttccac aagggaacgg
     3601 gcggattctt ctccagcgag gactggctga cccacgatta catcgagcag gacagaaccc
     3661 agtacggggc gacgatcggc ggcccgatca tcaaggacaa gacgttcttc ttcgccacgt
     3721 tcgaaggcgt catggaaaac tacggcagcg ccacgccgta ttatttcttc acgcccgacg
     3781 ccatcaagag ggccggcccg accgccaagg tcttcttcga caaagtcggc tcgaactatc
     3841 ccgttccgac gtatgatttc atcgacgtcg acggggacgg ctcgatcgac gccggccggt
     3901 atatttggga cggcaccggc aagtaccggg gctacaccgg cggcttgaag atcgaccaca
     3961 tcttctccga gaaggacaga atcgccgtcc ggtggctgtt caacacctac accgacaagt
     4021 gggatttcgc caacgttccc ggcaacatca aggaagtccc ctatgcctac cacaccggcg
     4081 gcctcacgtg gctgcatctt ttcagcccga cgatgtacaa cgaactccgg ctgggctacc
     4141 acagagacta cgccgactgg aagcgggtcg cccccgagat tcctcagctc ggcggctaca
     4201 cctatttcaa cgacggcgtc cacgccatcg gcgactgggc caacatgccc caggaattca
     4261 ccaacaacac ctatcagctg gtcgacgtcc tgaacttcca ggccggcaac cacagcatca
     4321 aggtgggcgg cgagctccgt ctttggaact ccgactccat gtttgacgcc aacgtcgcgg
     4381 gagcctatta cttcttcgat tcgacctggt tcctgtacga cctcggcgcc tactacctgt
     4441 acatcggcgc cgatcccccc gatccgccga aggacaaccc ctatgtcaag ggcgatccgt
     4501 ggggcgagtg gaaacagggc gattcccgga gaaaatggca gggccttgaa ggcggcgttt
     4561 tcgtccagga cgactggcgg gtgaccgagc ggttcaccct ctccctcgga ctgcggtggg
     4621 aatactacgg cgtccccgag gagaaatccg gcaagggcat caacatgccc gctttcggca
     4681 ccaaggccgg ctttgaatcc aagcaggtca tcgagggcgt cgagggcgag gaaggcgtcc
     4741 ggtatctcat tttcgacggc cgccagctcc tcggcaaggg cctctggaac ccctactacc
     4801 tggcgttcgc tccgaaagtc agcttcgctt gggacgtgac cggcgacggc aagacgtccc
     4861 tccgcggcgg cttcggcatt tcctacgacc ggacgttcaa caacacgtat gagaacgacc
     4921 ggttcaacta tcccgacttc acgttcgtcg gcctcctcgg gatcctggac ggctccccgg
     4981 ccatcgacgt cgcttatccc gcggtcgtcc cgatggccaa cgtggccggc tacggcgcga
     5041 gcctgcgctg gatgctgccc gatctgaagc cgcagaaggc ctataactgg ctgttcggca
     5101 ttcagagaga gctgtcctcc aacgtctcga tcgagatcaa ctacaccggc tcccacggag
     5161 ccaatctcgg ctcgattcag cggcccaacc ggttcaccgg cgacaagctc gacgagaccc
     5221 ggaccggcat caacccctac tgcgcgatca cgggcgtcaa cctgagaacc cagaacctcg
     5281 agtccgacta caacgccctg acggtcacgc tgaacaagag attctccgac ggctggtcct
     5341 ggtacaccgc ctacacgttc ggctatgcct acgaccagaa ctccgactac ttcggcgaca
     5401 acacctccat ggaggccgtg tccaacgagc ggatcgacat ggagcgcggc ttcgcccagt
     5461 tcgaccggag acaccgggtc gtcggcggat tcgtctacga catcccctgg ttcaagaact
     5521 ccgacaactg gctcctgaag aacgtcatcg ccggctggca gatcgccgga aacttcgacg
     5581 tcacctcggg cgcgcccttc acgattcggg gcgcttcgtc gagcacggac tggaactatg
     5641 actgggacaa caacgaccgg ccgctgtggc tgggtgatga cgacaccgac atcatcactt
     5701 ggaccaacgg gcgtcccggt tgggacaaga gcaatttcgg catcccggtc aagcccgccg
     5761 gcccgaacga cctgagctac tacgaccaga acttcgccga gcggaaccag ttccggtggt
     5821 tcccgaccca caacatcaac atcgccctgc agaaatactg ggtcctgaac gtcggcggca
     5881 aggaattcac catccagggc attttcgagg tcttcaacct gctcagaagc tacttctggg
     5941 cgttaccgtc cacgtcctgg accgcggccg acttcggcgt ttcgacgaga atgaacggcc
     6001 gccggacctc ccagatctcg ctccggatca tgttctaagg cacgcgcaca agcaccacgc
     6061 acaagttcaa aaccccgccc cggtcacccc ggggcggggt tttttttcgg ctcgatcttt
     6121 gctatattca acatatgcgc gacacggtga atatcaaacg tttttcggaa cggccggccg
     6181 gccgccttcg tctccataaa ggcctcgtct ttctcggcgt tctcctctgg gccggggccg
     6241 gcttgcggct cttcggcgcc ccccaaggca aggtccggcg ggacgccggc ctgaacatcc
     6301 tcctggtcac cctggacacg acccgggccg accgcatcgg cgcctacggc tgggccaagg
     6361 cgaaaactcc ggtgatcgac accctggccg ccggaggcgt gcggttcgcc aacgcctact
     6421 gcccggcgcc cctgaccctt ccctcccacg cctcgatcct gaccgggact tatccccttt
     6481 accacaaggt ccgcaacaac ggttcttatt acctgggtcc ggaggccgtc accctggccg
     6541 agcgtctcaa ggaaaaagga taccggacgt cggccgtcgt cgcctccttc aacgtcgact
     6601 cccggttcgg cctggaccag ggattcgact tctacgacga caagttcagc gacgacgaga
     6661 tgctcaagac gttccggtcc gaaaggcggg cggacgaggt ggccgacgcc ttcctgtcct
     6721 ggttcgaggg gcacgcccag gaaaagtttt tttcctggat ccatttttac gatccgcaca
     6781 tgccccatga cgtcccgtct ccctacaagg aggaattcgc cggggatctc tacgacggag
     6841 aaatcgccta catggaccat cacttcggcc ggatcatcga ccgcttgagg gagacgaagg
     6901 tcctggacaa gactctgatc gtcatcgccg gggaccacgg cgaggccctg ggggacaagg
     6961 gcgagctgga ccacggcatc ttcatctatg acgtgacgat gaaagtcccc ctcatttttt
     7021 acgggccgaa ggtcctgccc gccggacggg tctccgcttc ccgggtgcgc ctgatcgacg
     7081 tcatgccttc gattctcgag atggccggga taccggcgaa caaggagatc cagggggcga
     7141 gcctcctgcc cttcatcgag gggaaacgga aggacgattt atcgtgctac ctggagagct
     7201 actacccgct ggaaacctac ggctggtcgc agcttctagg cttcatcgac ggagactgga
     7261 aatacatcca ggcgccccgc tcggagcttt acaacctcaa gcaggacgcc cgggaggaaa
     7321 caaatctctt cccgcaagaa agcaaaaccg ccgccgcgct ggataaaaag ctcctggccg
     7381 cgatccggga ttattcctcg aaggcggaag cgggacggag aaagctgagc cgggaggagg
     7441 aggagcggct gcggtctctg ggctacgtcg gctccgacgc ctcggtgaaa atccccaa
//
LOCUS       DAOOXH010000105         7386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_11344_length_7386_cov_8.033693, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000105 DAOOXH010000000
VERSION     DAOOXH010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11344_length_7386_cov_8.033693"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PKV64_10080"
     CDS             <1..835
                     /locus_tag="PKV64_10080"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPN17459.1"
                     /translation="MLVLGLIVLALLLTKKMKDSLKIPLLLLSTFLFGFAPNLPVALF
                     KKFSMHPSPICAATKSILYGLGMPLLVTMAVIFFLTLVGPKLFCGWICPVGAAQELVA
                     MLADKLKIRRIKWNFIFTQTVRIGIFLLFVFLSATAVYHTYVEEQKVALSIYDPINAF
                     HGFEFTAQPSFFAAFLHYLPLLLTLILAFKYYRPFCYLVCPVGLFTNLLEQVAVFRVT
                     LKKPGCTDCGVCEKKSPCPTVPEILKRAVLRPDCFACTACVNACKECRALEYGTRRVS
                     R"
     gene            complement(883..1263)
                     /locus_tag="PKV64_10085"
     CDS             complement(883..1263)
                     /locus_tag="PKV64_10085"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPN17460.1"
                     /translation="MERITEIYVVLENKPSTLGDLCSYLAENEINIEAIGVFHDTAKL
                     YVKNLNKTVKLLQKLNYIIDLRDVLLIDIENRPGALADLTTKLGDEGINIDYLYGTVS
                     HKPNMNTVILDVNKIDEAMRVLEA"
     gene            complement(1407..2825)
                     /locus_tag="PKV64_10090"
     CDS             complement(1407..2825)
                     /locus_tag="PKV64_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPN17461.1"
                     /translation="MPSIITLEMRAALESVVRPENVLTDADTLAAYGHDEFSLSDIRQ
                     PAGIVVRPKTADEVAAVLRLAGSARIPVTARGGATGLCGGCVPSPGGIVLSMERMNRV
                     LEIDPVNQVAVVEAGVPLHELEKAVAEAGFDFPPHPGEESAAVGGMIATNAGGSRAVK
                     YGVIRNYVRGLEVVTAAGGILTLGGKLVKSSSGYNLMHLFIGSEGTLGIITKAVLQFR
                     VKPALTRSLVIPFESLAAAIESVPVLLNRDAVPLALEFVPLDVLQITEGFLGRTWPCH
                     AGSVHLFVILDAVSEEDMERQTESVAGVALERGALDVFVADTAAKQDNVLEIRSKIYE
                     ALKAHTIEIMDVSVPRGEIAAHVEKVEEIGAKFGVWLPTFGHAGDGNVHTHLMKARCE
                     GGRIVPLAEEEWRASLEAVRTEIYADGLARGGVVSGEHGIGLVKKKYLAESLPPAQIE
                     LMRGIKRAFDPAGLLNPGKIFD"
     gene            3022..3600
                     /locus_tag="PKV64_10095"
     CDS             3022..3600
                     /locus_tag="PKV64_10095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPN17462.1"
                     /translation="MDEHNLIRLARAGDATAWRSLFDTHHRRVFATAYRFLANQADAE
                     DVLQETFVRAYRSLDGFDSSYAGGFAAWIGRIGANCSLDAIRRKKKTRDTLSLEDGAV
                     PPPPDSGHGTDPERSTGNREIRARIENALNRLTPKQRLIFILRHYEGYTTREIAGMID
                     VTEGSVKTHLFRAVGALRKSLRRVALEDRYEM"
     gene            3590..4318
                     /locus_tag="PKV64_10100"
     CDS             3590..4318
                     /locus_tag="PKV64_10100"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPN17463.1"
                     /translation="MKCKNARRAIVLDYYGELPPAEKAGLEEHLRTCRKCGAEREETV
                     RVFSLLEANPPGDIPVPDTGLVWSRIENRLDPKRAPERRPAPAWNIRQWAMAGAALAL
                     VLAAGIFIGRRASPPPSPPAASPSSGPVRTTAALKPVLAGHLEDLKPLLLDYANYTPD
                     DAAGATVVIDEEFLRALLFQNVLLRKALAGSDPAAAELLDDCDLILKEIINRDAPAAA
                     SPEDIRELIRGRDVLFKLEIIKRT"
     gene            4329..5609
                     /locus_tag="PKV64_10105"
     CDS             4329..5609
                     /locus_tag="PKV64_10105"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPN17464.1"
                     /translation="MIKKTRIVLLVLALFLAAGSTGKAAVWTLIQDSVQAAEQAGKKA
                     RQTEQEARARALYEAAKKNVYNKTYEAAIAQFNDLAAKYAASAYGQEALYWLGYSLDK
                     QAASLGDVKQRLEMQQAAMEHLETLMKDFPANAWVKDAKILQLQIAEELVRAGLGKYR
                     RYIDQSVQEALESSLPVLEQFAVPPVPPIPPEPPVPSIPSKPLDPETELKLVALDALM
                     GMDEEKAFPILEKMILSGQSPELREKALFILGQSDAAKVVPVLIQIAEKDASPKMRKS
                     AIFWLGQRDEEQALAALIKIFETADAETKTVLLMAFAENKNPKGIAKITEIAKTEKDP
                     EVRARALFWLGETEGEKAFPVLLESYRTTDNVQIKKQIIMALAQSQGPKAVSTLIDLA
                     KKETNFDLKKQIVFWLGQSDSPEAAKFLQELIDK"
     gene            5606..6748
                     /locus_tag="PKV64_10110"
     CDS             5606..6748
                     /locus_tag="PKV64_10110"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPN17465.1"
                     /translation="MTARIMNAEKAAALFGAVAFLLAAVVCTAPAGPTPDVGGTGFLK
                     GRVHDAARAGASLEERFAAARSEAARSSPSGTFFAAARFESVSRIHFGRPDGAGPLTV
                     ASGDSRIIIRETSGDDPSIREGDEKRTPSPAAWLILSDVKTGRVVDATILDPERTYAF
                     DSQAVFWLGNPPGAESLSFIETRFQAASDPEVRERMLLVLSRHNDSRVYGLLRRIALE
                     DREMDVRRSAVFWLGTIPDPRAPAGLKEIYRLDRDPEIKERVVFALSLRPEKEAVLEL
                     IRIARKDADDSVRRNAVFWLGQKASAESVRALKDIIESPGEERQLKEQAVFAVSQLPP
                     ERAVPMLIDIARTHRSSSVRKKALFWLGQTGDPAALKLFEEILLKK"
     gene            complement(6797..>7386)
                     /locus_tag="PKV64_10115"
     CDS             complement(6797..>7386)
                     /locus_tag="PKV64_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17466.1"
                     /translation="LKVARSERQLKLMLNSQQEEYLACAFYGCDRGYVESLCDPKARE
                     KESLYLLEDCFDDSLLSTDTPFVFDHDFEPPGYTSVFLTGGTGYIALHVTPRPIGSTP
                     NFVEFCAYIHPSMWVFDRESLIHVMKPVLRLFNPKKAEIHYRISSRNIAGDKNQHRKF
                     VRKSLVGLGYTTEEVVAYYDEYFSRTFFHLNIEKK"
BASE COUNT         1395 a         2419 c         2220 g         1352 t
ORIGIN      
        1 catgctggtt ctcggcctga tcgtcctggc ccttctcctg acgaagaaga tgaaggacag
       61 cctcaagatc ccgcttctgt tgctgtcgac gtttcttttc gggttcgccc ccaatctccc
      121 ggtcgccctg ttcaagaaat tttcgatgca cccctcgccg atctgcgccg cgaccaagtc
      181 gatcctctac ggcctgggga tgccgcttct ggtcaccatg gcggtcattt tcttcctcac
      241 cctcgtcggc ccgaagctgt tctgcggctg gatctgcccc gtcggggccg cccaggaact
      301 ggtcgccatg ctcgcggaca agctcaagat ccggcgcatc aagtggaatt tcatcttcac
      361 ccagaccgtc cggatcggga tctttcttct cttcgtcttc ctgagcgcga ccgcggtcta
      421 tcacacctat gtcgaggagc aaaaggtcgc cctctcgatc tacgatccca tcaacgcctt
      481 ccacggattc gaattcaccg cccagccctc gtttttcgcc gcctttctcc attacctccc
      541 cctgcttctg accctgatcc tggcgttcaa atactaccgg ccgttctgct acctggtctg
      601 cccggtcggc ctgttcacca acctcctgga gcaggtcgcc gtgttccggg tcactctgaa
      661 aaagcccgga tgcaccgact gcggcgtttg cgaaaagaaa agcccgtgcc cgacggtccc
      721 tgaaatcctg aaaagagccg ttctccggcc ggattgcttc gcctgtacgg cgtgcgtgaa
      781 tgcctgcaag gaatgccggg ccctggaata cggaacccgg cgcgtttctc gctgaccggc
      841 ggacaccgga ccgcggggcc cggcccccga ttccgccggc ggctaggctt cgagaaccct
      901 catcgcctcg tcgatcttgt tgacgtcgag gatgacggtg ttcatattcg gcttgtggct
      961 gaccgttccg tagagataat cgatgttgat cccctcgtcg ccgagcttgg tcgtcagatc
     1021 ggccagggcc ccgggacggt tctcgatgtc gatcagcagg acatcccgca gatcaatgat
     1081 atagttcagt ttctgcagca atttgacggt cttgttcaga ttcttgacgt acagcttggc
     1141 cgtgtcgtga aaaacgccga tcgcctcgat gttgatctcg ttttcggcca ggtaggaaca
     1201 cagatccccc agcgtcgacg gcttgttttc caggacgacg tagatctcgg tgatgcgttc
     1261 cataggacct ccttatgcga tttcgtccgt ggagggacga cgacacgctt cttcgattcc
     1321 gggagaaaac gctgcgcctt gaccggcgcg gggagttgtc atgggcggtg tgattcaata
     1381 taggtcaaga ccccctaaaa agcaaatcaa tcgaagattt tgccgggatt gaggaggccg
     1441 gccgggtcga aagcccgttt gatcccccgc atgagctcga tctgggccgg gggaaggctt
     1501 tcggccaagt attttttctt gaccaacccg atgccgtgct cccccgagac gacgccgccg
     1561 cgggcgaggc cgtcggcgta aatctccgtc cggacggcct caagggaagc ccgccattcc
     1621 tcctcggcca ggggaacgat ccgccccccc tcgcagcgcg ccttcatcag gtgggtatgg
     1681 acgttgccgt cgccggcgtg gccgaacgtc gggagccaga cgccgaactt ggccccgatc
     1741 tcctccactt tctcgacgtg ggcggcgatt tccccgcggg ggacggacac gtccatgatc
     1801 tcgatcgtgt gggccttgag ggcttcgtag atcttgctcc ggatctccag gacattgtcc
     1861 tgcttggccg ccgtgtcggc gacgaaaaca tcaagggcgc cgcgctccag ggcgaccccg
     1921 gccaccgact ccgtctgccg ttccatatcc tcctcggaaa cggcgtcgag gatgacgaac
     1981 aggtggaccg atccggcgtg gcagggccag gtccgtccca ggaatccctc ggtgatttgg
     2041 aggacgtcga gggggacgaa ttccaaggcc agggggacgg cgtcccggtt gagaagaacg
     2101 ggaacggatt cgatcgccgc cgcgaggctc tcgaagggga tgacgagcga gcgggtcaag
     2161 gccggcttga cccggaattg aaggacggcc ttggtgatga tcccgagcgt cccctccgag
     2221 ccgatgaaca agtgcatcag gttgtaaccg ctgctcgact tgacgagctt gccgccgagg
     2281 gtcagaatcc cgccggccgc ggtcacgacc tccagccctc ggacgtagtt gcggatcact
     2341 ccgtatttca ccgcccggct gccgccggcg ttggtcgcga tcatcccgcc gacggccgcg
     2401 ctctcctccc ccgggtgcgg cgggaaatcg aatcccgcct cggccaccgc tttttcgagc
     2461 tcgtgaagag ggaccccggc ttcaacgacg gccacctggt tgaccggatc gatctccagg
     2521 acccggttca tccgctccat gctcaggacg atcccgccgg gagaggggac gcatcctccg
     2581 cacaacccgg tcgctcctcc ccgggccgtc acgggaatgc gggcggaacc ggccagacgg
     2641 aggacggccg cgacctcgtc cgccgtcttc ggacggacga cgattccggc cggttgccgg
     2701 atatcgctca gggaaaattc gtcgtggcca taagcggcca gggtgtcggc gtcggtcaga
     2761 acgttttccg gccggacgac ggattccagg gcggcccgca tttcgagagt gatgatggac
     2821 ggcatggcga ctcctctcat gtggagggga ttataccctg attcccttcg gtcatgtagg
     2881 gcggcggagc cgcctgactc cgaagcgtcg tcgaggacgg caaggccgcc acccaggcta
     2941 tcctcgcacc cccctctttt aaggtatgat tttgtcaacg aatcccaagc gcagggagta
     3001 tatccggatg agaaggcccc tatggatgaa cacaatttaa tccggttggc ccgggcggga
     3061 gacgcgaccg cctggcgcag cctcttcgac acgcatcacc ggagggtttt cgccacggcc
     3121 tatcgctttc tggccaacca ggcggatgcc gaggatgtcc tccaggaaac gttcgtccgg
     3181 gcctatcgca gcctggacgg attcgactca tcttatgccg ggggcttcgc cgcttggatc
     3241 ggccggatcg gggccaactg cagcctcgac gccatccggc gcaagaagaa gacccgggac
     3301 accctttccc tggaggacgg ggccgtgcct cccccgccgg actccggcca tgggaccgac
     3361 ccggagcgct cgaccggaaa ccgggagatc cgggcccgga tagagaacgc cctgaaccgg
     3421 ctgacgccga aacaacggct gatcttcatc ctcaggcatt acgaaggata caccacccgg
     3481 gaaatcgccg ggatgatcga cgtgaccgag ggcagcgtga aaacccacct gttccgggcc
     3541 gtgggagcgc tgcggaaaag ccttcgccgc gtcgcgctgg aggatcgcta tgaaatgtaa
     3601 aaacgcccgc cgggccatcg ttctcgatta ttacggcgag ttgccgcccg ccgaaaaagc
     3661 cggtctcgag gagcatttgc ggacctgccg gaaatgcggc gccgagcggg aggaaaccgt
     3721 ccgcgtcttt tccctcctgg aagccaatcc gcccggcgat attccggttc cggacaccgg
     3781 gctcgtttgg agccggatcg aaaaccgcct ggatccgaaa cgggcgccgg aacgccggcc
     3841 ggcgccggcc tggaacatcc ggcaatgggc catggccggg gcggctctcg ccttggtcct
     3901 tgccgccgga attttcatcg gacggcgggc ctctccgcct ccctcccctc ccgccgcgtc
     3961 tccgtcgtcc ggacccgtcc ggacgacggc cgccctgaaa cctgttttag ccggacacct
     4021 ggaagacctg aagccgctcc tgctcgatta tgccaactac accccggacg acgccgcggg
     4081 cgcgacggtc gtcatcgatg aggaattcct ccgggccctt ctttttcaga acgtcctgct
     4141 gcggaaagcc ctggccggct ccgacccggc cgcggcggag ctcctggacg actgcgactt
     4201 gatcctcaag gaaatcatca accgggacgc tccggcggcg gcctcccccg aagacatccg
     4261 ggagctgatc cgcggccggg acgtcctgtt caagctggaa atcatcaaaa ggacatagga
     4321 gaaaaatcat gataaagaaa acgcgcatcg ttttgctcgt cctggccctc tttctcgccg
     4381 ccggatcgac cggaaaagcg gccgtctgga cgctcatcca agacagcgtt caagcggccg
     4441 aacaggccgg gaaaaaagcc cggcaaacgg aacaggaagc ccgggcccgg gccctttacg
     4501 aggccgccaa gaagaacgtt tacaacaaaa cctacgaggc ggcgatcgcc caattcaacg
     4561 atctggcggc caagtacgcc gcgtcggcct acggccagga ggcgctgtac tggctgggat
     4621 acagcctcga caagcaggcc gcgtccctcg gagacgtcaa gcagcgcctg gagatgcagc
     4681 aggccgcgat ggagcacctc gaaaccctca tgaaggactt cccggccaac gcctgggtca
     4741 aggacgcgaa aatcctccag ctccagatcg ccgaggagct cgtccgcgcc ggcctcggca
     4801 agtaccgccg gtatatcgat cagagcgtcc aggaagccct ggaaagcagc ctgcccgtcc
     4861 tcgagcagtt cgccgtcccg cccgttcctc caattccgcc ggagccgccc gtgccctcca
     4921 ttccctcgaa accgctggac ccggagaccg agctcaagct cgtcgccctg gacgccctca
     4981 tgggcatgga cgaggaaaag gcgttcccca tcctcgaaaa aatgatcttg agcggccaaa
     5041 gccccgagct tcgggaaaag gccttgttca tcctcggcca gagcgacgcg gccaaagtcg
     5101 tccccgtcct catccaaatc gccgaaaagg acgcgtcccc caaaatgcgg aaaagcgcga
     5161 tcttttggct cggccagcgg gacgaagaac aggccctggc ggccttgatc aagatttttg
     5221 aaaccgcgga cgccgagacc aagaccgtgc ttctgatggc cttcgccgaa aacaagaatc
     5281 ccaaggggat cgcgaagatc acggaaatcg ccaaaaccga gaaggatccc gaggtccggg
     5341 cccgggccct gttctggctc ggagaaaccg aaggtgaaaa agcgttcccc gtcctcctcg
     5401 agtcctaccg gacgacggac aacgtccaga tcaaaaagca gatcatcatg gccttggccc
     5461 agagccaagg ccccaaggct gtttcgacct tgatcgacct ggcgaaaaag gaaacgaatt
     5521 tcgacctcaa gaagcagatc gttttctggc tcggccagtc ggacagcccc gaggcggcca
     5581 aattcctcca ggaactgatc gacaaatgac ggcccgaatc atgaatgctg aaaaagcggc
     5641 ggcccttttc ggggcggtcg cttttctcct cgccgcggtc gtttgcacgg ccccggccgg
     5701 tccgacgccg gacgtcggcg ggaccggttt tctcaaaggc cgcgtccatg acgccgcccg
     5761 ggccggggcc tcgctggagg agcggttcgc cgccgcccgg agcgaggcgg cccgctcttc
     5821 cccttccgga acgtttttcg ccgcggcgcg gttcgaatcc gtgagccgga tccatttcgg
     5881 ccgtcccgac ggggccgggc ccttgaccgt cgcctccggc gactcccgga tcatcatccg
     5941 ggaaacatcg ggggacgacc cgagcatccg ggagggcgat gaaaaaagga cgccctcccc
     6001 ggccgcctgg cttatcctca gcgacgtcaa aacgggccga gtcgtcgacg cgacaatcct
     6061 ggatcccgag cggacgtacg cgttcgattc ccaagccgtc ttttggctcg ggaatccccc
     6121 cggcgctgag agcctgtctt tcatcgaaac gcgtttccag gccgcttccg atcctgaagt
     6181 ccgggaacgg atgctcctcg tcctcagccg ccacaacgac tcccgggtct acggcctcct
     6241 gcgccggatc gccctcgagg accgggagat ggatgtccgt cgctcggctg ttttctggct
     6301 cgggacgatc ccggatccgc gcgccccggc cggtttgaag gagatttatc gcctggaccg
     6361 tgacccggaa atcaaggagc gcgtcgtttt cgccctctct ctccgcccgg aaaaagaagc
     6421 cgtcctggag ctgatccgca tcgcccgtaa ggacgcggat gattccgtcc ggcggaacgc
     6481 cgtcttctgg ctcggccaga aggcctcggc cgaaagcgtc cgggcgctga aggacatcat
     6541 cgagtcgccc ggcgaagaac gccagctcaa ggaacaagcc gtcttcgccg tcagccagct
     6601 tcccccggaa cgggccgtcc cgatgctgat cgacatcgcc cggacccacc ggtcctcctc
     6661 ggtccggaaa aaagctttgt tctggctcgg ccagaccggc gatccggcgg ctctcaagct
     6721 attcgaagag atcttgctga agaaataatc cgatccctcg gagccggggc gggacggcct
     6781 ccctcccggc tcatcctcat ttcttctcga tgttcaggtg aaagaaggtc cggctgaagt
     6841 attcgtcgta ataggccacg acttcctcgg tcgtatagcc caggccgacc aagctcttgc
     6901 ggacgaactt ccggtgctgg ttcttgtcgc cggcgatgtt ccgcgaggaa atccggtaat
     6961 ggatctcggc ctttttcggg ttgaacagcc tcaaaaccgg cttcatgacg tgaatcagcg
     7021 actcccggtc gaacacccac atgctgggat ggatatacgc gcagaactcg acgaagttgg
     7081 gcgtcgatcc gatcggccga ggcgtcacat ggagggcgat gtagccggtg ccgccggtca
     7141 ggaaaacgct ggtgtagccc ggcggctcga aatcgtggtc gaagacgaag ggcgtgtcgg
     7201 tggagagaag gctgtcgtcg aagcagtcct cgagcaggta gagcgattcc ttttcccggg
     7261 ccttgggatc gcaaagggac tcgacatagc cccggtcgca gccatagaag gcgcaggcca
     7321 ggtattcctc ctgctgggag ttgagcatca gcttcagctg gcgctcgctc cgggccactt
     7381 tcagac
//
LOCUS       DAOOXH010000106         7328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_11462_length_7328_cov_6.690499, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000106 DAOOXH010000000
VERSION     DAOOXH010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 7328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7328
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11462_length_7328_cov_6.690499"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="PKV64_10120"
     CDS             complement(<1..1083)
                     /locus_tag="PKV64_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN17467.1"
                     /translation="MKIKAFNENGLYENSFLLLREYENERFGKSTEAERVLFCLNAAS
                     ASWIIGRPKTAMEYAQKANLWSVSNDDAEMEERSRRIVNAIRLYNSGKNKRLQGDGLG
                     SRDAFHKAIEESRFINGIEYEMKCLRQMSLSYLDEYQYEDFYHCNEDALRIARKLRSF
                     RDEGYCLNNIGLYYYEKDNYSEALKHYLSSLEIAERTGNLRAKSECSTNIGVIYKTIG
                     EYDTALEYFHSALEIDKVIDHKEFISIDLANICSVHRGKWIESNSDHDLQAAYDTLRE
                     CYEAYSPGTHDFIAARIANNLGALAIDLKNYEKGTQYYQEGLAAAGRNGDKVAICSIT
                     RNIGSALMLMGREEEALSFFRDAISLA"
     gene            complement(1820..2221)
                     /gene="ndk"
                     /locus_tag="PKV64_10125"
     CDS             complement(1820..2221)
                     /gene="ndk"
                     /locus_tag="PKV64_10125"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPN17468.1"
                     /translation="MERTLAIIKPDSIKKNIIGKIIDRIEAEGFRIVEMRLVHLKAEE
                     AMNFYAVHREKPFYADLVAFMSSGDSVVLLLERENAIARWREVMGATDPAKAAPGTLR
                     RLYGFSIERNAVHGSDARDTAAAEIGFFFKS"
     gene            2407..2931
                     /locus_tag="PKV64_10130"
     CDS             2407..2931
                     /locus_tag="PKV64_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17469.1"
                     /translation="MNQERPPLFVPALIGGTAAGVLSAVPLLNCLCCLWIIGGAMLAA
                     ALWSKDSPVSLTSGDGALVGAFTGLFAAAAHSLINIPLAAVNSAFFRNVFTRLSTYID
                     EMPQGWEDLFKQQSAGSFQIAGFFLGLIVSAAVFAVLGTLGGILGVALFGKKTPPSSF
                     VPSPPAPPTAGPSI"
     gene            2936..3877
                     /locus_tag="PKV64_10135"
     CDS             2936..3877
                     /locus_tag="PKV64_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HPN17470.1"
                     /translation="MRKRLKTQVIVNPASAKGETRKRWSQIREGLRHFIQEYKIEFTE
                     RPFQAIDIARSSLRDGTELIIGVGGDGTLNEIANGFYDGEKPINADAALGIVPSGSGC
                     DLTRSLNIPVHLKGAMTVLQDAGSIPIDIGKVSYQSTEGQEASRYFLNVADFGLGGEV
                     IREVNRRRLERRASSYVKCLISTVLRYRSKKIRVRIDDCDLPRDEYMIGAIANGRVFG
                     KGMKIAPQASLDDGLFDAVMVKGMKLVEFCRQGWRLINGSHLTYRKVDLIRGRQVEAV
                     PEEDEEPILLEMDGEQLGTLPAVFELIPRGLLVKGYL"
     gene            complement(3923..4516)
                     /locus_tag="PKV64_10140"
     CDS             complement(3923..4516)
                     /locus_tag="PKV64_10140"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPN17471.1"
                     /translation="MSKAVVRIAAGWAVAGFLLLLLPAPGHSAGFRSRMKEKYDPYIQ
                     TIAAEHGVDADFVHAVITAESAYNRYAISRAGAQGLMQLMPSTASAYGVKDVFDPEDN
                     IRGGVKFLKMLLRLYNGNLKKTLAAYNAGQEAVKKYGGIPPYAETRTYISRVMASYQA
                     PAAPGRTRIYEFRDASGRIILTNDPKLAAQYSAAGVS"
     gene            complement(4595..7231)
                     /gene="alaS"
                     /locus_tag="PKV64_10145"
     CDS             complement(4595..7231)
                     /gene="alaS"
                     /locus_tag="PKV64_10145"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPN17472.1"
                     /translation="MNAQDIRETFLDYFSRRGHKVVASSSLLPKDDPTLLFANAGMNQ
                     FKNVFLGLERRSYVRAASIQKCMRVSGKHNDLDQVGRTAKHHTFFEMLGNFSFGDYFK
                     KEAVAFAWELITEVYHIDKDRLYATVYEDDEEADRIWREEIGLPADRVFRFGTTDNFW
                     EMGDTGPCGPCSELHYDFAPLLEPGAPRDLIESGSDRFVELWNLVFMQFEQDGKGGRR
                     PLPRPSIDTGMGLERLTAVLQGKTSNWDTDLFMPLIEDVSRKTGREYPSGDESDVSIR
                     IIADHARSIAFLIGDGIMPANDGRGYVLRRLIRRAFRQGQKLGFEEPFVHALLGVVAG
                     IMRDAYPELTSSLPLISRICRAEEERFSRTLSSGFRYFQEFARETRKAGSRVLAGEAA
                     FKLYDTFGFPLDLTRELAQEEGITVDEAVFQNELERQKTRARRTWKGDARMKEKKAYA
                     GISPSSVKYVGADKGEVAESRVLALFKEGKRVNAIKEGETGEAVLSLTPFYAEAGGQV
                     GDAGLIKGSHASALVETAYYPVPDLRSHKIRMLSGSLAEGDMVAAAVDEARRAATRAN
                     HTATHLLHAALRSVLGEHVKQAGSLVGPDRLRFDFTHFQALAEEEITGVETIVNEKIR
                     ENLPVGIDEMSLEEGLRSGAMAIFEEKYGERVRLVSVGEYSRELCGGVHAGTTGEIGV
                     FKIVGESSVAAGMRRIEALTGEEAIEYIQENDRLIHAAAAVLSVSRKDLLAQIAKLQE
                     HLKEKEHEIRTLRRSKVRETGGADETPMREVKGVSVLVRRRDGLGPAEARAEMDDIKN
                     RLKSGVGLLGTVHEGKALLIAGVTKDLTARIQAGALIKRLAPLIGGGGGGRPDFAQAG
                     GAGVDSLDKALSEGFRVIEETL"
BASE COUNT         1483 a         2268 c         1964 g         1613 t
ORIGIN      
        1 ggccagggag atcgcgtccc ggaagaagct taaagcttcc tcctcccggc ccatgagcat
       61 caaggcggat ccgatatttc tcgtgatgga acagatggcg actttatcgc cgttcctgcc
      121 agctgcggca agcccttctt ggtaatactg cgttcctttt tcataattct tcaaatcaat
      181 cgccaacgcg ccaagattat tcgcgattct tgcggcaatg aaatcgtggg tccccggcga
      241 ataagcctca taacactctc tcaacgtgtc ataagctgcc tgcaaatcat gatcagagtt
      301 cgactcaatc cacttgcccc gatgaaccga acaaatattg gctaaatcga tggagatgaa
      361 ttctttatgg tcaataacct tatcgatttc caaggcggaa tgaaaatatt cgagagccgt
      421 atcatattcg ccgatcgttt tataaataac gccgatgttc gtcgaacatt cgctctttgc
      481 tcttaaattt cccgtccttt cggcgatctc taaagagcta aggtagtgtt tcaatgcctc
      541 ggaataatta tctttttcat aataataaag accgatattg ttcaaacaat atccttcgtc
      601 tcgaaaagaa cgcagctttc gagcgattct taaggcatct tcattacaat gataaaaatc
      661 ttcatattga tattcgtcca gatagctgag gctcatttga cgaaggcatt tcatttcata
      721 ttcgatacca ttgataaatc gagattcctc gattgcttta tgaaatgcat cccttgatcc
      781 caggccatcc ccctgaagac gcttattctt cccagagtta taaaggcgaa tcgcatttac
      841 gatgcggcgg cttcgctcct ccatttcagc atcgtcgtta gagacggacc ataaatttgc
      901 tttttgcgcg tattccatcg cggttttcgg cctgccgatt atccatgacg ccgaagccgc
      961 gttgaggcaa aaaaggacgc gttccgcctc cgtcgatttc ccaaagcgtt cgttctcata
     1021 ttcgcgcaag agtagaaacg aattttcata aagcccattc tcattgaaag ctttgatttt
     1081 cacgaggagt tcatcaagcg atttaatgac tgttcccctc gaataaaccg ctgaagccgt
     1141 tattaataat aaagaagtga tcgttattag gccgtttcga aaaatcatat cttttaaaag
     1201 aaggcgtttt tatcccattt ctattgatgt cgtaattctt cccttgggcg cggctagatc
     1261 tttataggcg gacgttcccc cccaaaaaag aatctatacg aaaaagaatc ctcatttctt
     1321 ttcatgggca gattttcaca atttccatat cctccagaac gaaactaagg acgttttccg
     1381 tcctattcat agttttctcc cccagaagcc ggcaagccca ttcgggggaa atcgcctgga
     1441 cagcgagcca gaaggaccat tcccaaccga atccgccgcc ggcccgcccg gacggaacgt
     1501 acctctccaa gccgcaccag cctggaacca ccgggatgaa cgagccgagg cacaataaag
     1561 cggccgcttt tttcaaaaag cctttcaaaa acacgaaaat tcccctttct gaagatcatt
     1621 tcgtcaataa gaaagacgtc ttgagcggat ttttgtatca gttcctcttc cccttttgac
     1681 tttccctgcc gcgatttcgc cgacaagcct attcaatcaa tgataaaaag tcaagccatg
     1741 agggcatttt tttacataaa cagcgcgctc cggccgcggc cggtgctagg gaagaaacaa
     1801 gggccgggcg attcccgggc tatgatttga aaaagaatcc gatttccgcg gccgccgtgt
     1861 cgcgggcgtc ggagccgtgc acggcgttcc gttcgatcga gaatccgtac aatctccgca
     1921 gcgttcccgg cgccgccttg gccgggtccg tcgcgcccat gacctcgcgc cagcgggcga
     1981 tcgcgttttc ccgctccaag agaagcacga cggagtctcc tgagctcatg aaggcgacca
     2041 agtccgcgta aaacggcttt tcccggtgga ctgcataaaa attcatcgcc tcttcggcct
     2101 tcaaatgaac cagccgcatc tcgacgatgc ggaaaccttc cgcctcgatg cggtcgatga
     2161 tttttccgat gatgtttttc ttgatgctgt cgggcttgat gatcgccaat gtccgttcca
     2221 tttttctctc ctgaccggta aaagcccaag aatcatatcc ggaagatcgg ggaacgtcaa
     2281 ccaacccccc tctcccgacc gagaggactc ccgcccgagc ccgaagaaac gcgcattttc
     2341 ccgtcaagtt gataaattgc gcggaaaatc ttagaatgag atgcttaaaa cggctaggag
     2401 acttcgatga accaagagcg accgcctctt ttcgttccag ccctgatcgg cggaacggcc
     2461 gccggagttc tctccgccgt tcccctgctt aactgcctgt gctgcctctg gatcatcggc
     2521 ggcgcgatgc tggccgccgc cctctggtcc aaggattccc ccgtctccct gacttcaggc
     2581 gacggagcct tggtcggcgc cttcaccggg ctattcgccg cagccgccca ttccctgatc
     2641 aatatccccc tggccgcggt caattccgct tttttccgga acgtcttcac ccgcctttcc
     2701 acctacatcg acgagatgcc tcaagggtgg gaagatttgt tcaaacaaca gagcgcgggg
     2761 tccttccaaa tcgccggctt tttcctgggg ctgatcgtct cggcggcggt cttcgccgtt
     2821 ctgggaacgc tcggcggaat cctcggggtc gccctgttcg ggaaaaagac ccccccctcg
     2881 tccttcgtcc cgtctccgcc cgcccctccg acggcgggtc cgtcgatcta agccgatgag
     2941 aaaacgcctc aaaacccaag tcatcgtcaa cccggcctcg gctaaagggg aaacgcggaa
     3001 acgctggagc cagatccgcg aaggactccg ccatttcatt caagaataca aaatcgagtt
     3061 caccgagcgc ccctttcagg ccatcgacat cgcccgctcc tccctccgcg acggaaccga
     3121 gctgatcatc ggcgtcggcg gagacggcac cctgaacgaa atcgccaacg gcttctacga
     3181 cggcgaaaaa ccgatcaacg cggacgccgc cctgggcatc gtgccctcgg gctccggctg
     3241 cgacctgacc cgcagcctca acatccccgt ccacctcaag ggggccatga ccgtccttca
     3301 ggacgccgga tcgatcccca tcgacatcgg caaagtgtcc tatcaatcga ccgagggaca
     3361 ggaggcctcg cgctacttcc tcaacgtcgc cgatttcggc ctcggcggtg aagtcatccg
     3421 ggaagtgaac cggcgccgcc tcgaacgccg cgcgtcatct tatgtcaaat gcctgatctc
     3481 gaccgtcctt cgctaccgga gcaagaaaat ccgcgtccgg atcgacgatt gcgatcttcc
     3541 ccgggacgaa tacatgatcg gggcgatcgc caacggccgc gtcttcggca aaggcatgaa
     3601 aatcgcgcct caagccagcc tggacgacgg cttgttcgac gcggtcatgg tcaagggaat
     3661 gaaactggtc gagttctgcc ggcaggggtg gcgattgatc aacggttccc acctcaccta
     3721 tcgcaaggtg gatctgatcc gaggccggca agtggaggcc gttccggagg aggacgagga
     3781 gccgatcctc ttggaaatgg acggcgagca actaggcacc ctacccgcgg ttttcgagct
     3841 cattccccgc ggattgctcg ttaaaggcta tctctgaaat cggccgcgga tttctgtccc
     3901 ccggagcggg ggcctttctt ctttatgaaa caccggccgc gctgtattgg gcggccagct
     3961 taggatcgtt ggttagaatg attcggccgc tggcgtctcg gaactcgtaa atcctggttc
     4021 gtcccggagc cgccggggcc tggtaggagg ccatgacccg gctgatgtat gtccgcgttt
     4081 cggcgtaggg ggggattccg ccatatttct tgacggcttc ctggccggcg ttgtacgccg
     4141 ccagggtttt cttgagattt ccgttataaa gcctcaacag catcttcaag aacttgactc
     4201 cgccccggat gttatcctcg gggtcgaaaa cgtctttcac gccgtaggcc gaggccgtgg
     4261 acggcattaa ttgcatcagc ccctgggccc cggctctcga tatggcatag cgattgtagg
     4321 ccgattccgc cgtgatcacg gcgtgaacga aatcggcgtc caccccgtgc tcggccgcga
     4381 tggtctggat gtaaggatcg tatttttcct tcatccgcga gcggaagccg gccgaatggc
     4441 ccggcgcagg cagaaggagg aggaggaagc ccgcgacggc ccatccggcc gctattcgaa
     4501 ccacagcttt agacattgct gttgttattg taaaaccgtc ccggaagggg tcaatcaccc
     4561 ttaggggtga attcgggtgg gggttcgtcc cccttcagag ggtctcctcg atgacccgga
     4621 aaccctcgga aagagccttg tccagggaat cgacgccggc cccgccggct tgggcgaaat
     4681 cgggccgccc gcctcctccc ccgccgatca agggagccag ccgcttgatc agggcccccg
     4741 cctgaatcct tgcggtcaaa tccttggtga ccccggcgat aaggagcgcc ttgccctcgt
     4801 ggaccgttcc caaaaggcct acgcccgatt tcaaccggtt cttgatatca tccatctccg
     4861 cccgggcttc agccggcccc aggccgtccc ggcgccggac gagcacggag acgcccttca
     4921 cctcccgcat gggagtctcg tccgctccgc ctgtttcccg gaccttcgac cggcgcagcg
     4981 tccggatctc gtgctccttc tccttcagat gctcctggag cttggcgatc tgggccagga
     5041 gatccttccg ggaaacgctc aggaccgcgg ccgccgcatg gatcaagcga tcattttcct
     5101 ggatatattc gatcgcttcc tcgccggtga gggcctcgat ccggcgcatc ccggcggcca
     5161 cgctcgattc gccgacgatt ttaaacacgc cgatttcgcc cgtcgtcccg gcatggactc
     5221 cgccgcagag ctcccggctg tattccccga ccgagacgag gcgcacgcgc tctccgtatt
     5281 tctcctcaaa gatggccatg gccccggagc ggaggccctc ctccagagac atctcgtcga
     5341 tgccgaccgg gagattctcc ctgattttct cgttgacgat cgtttcgacg ccggtgattt
     5401 cctcttcggc caaggcttgg aaatgagtga agtcgaagcg cagccggtcc ggtccgacca
     5461 gcgagcccgc ttgtttcaca tgctccccga gaacgctccg aagggcggcg tgaagaagat
     5521 gggtggccgt atgattagcc cgggtcgccg cgcgccgggc ctcgtcgacg gccgccgcga
     5581 ccatgtcccc ctcggccagg gatcccgaaa gcatcctgat cttgtggctc cggagatcgg
     5641 gcacgggata ataggcggtt tcgacgaggg ctgaagcatg gcttcccttg atcagcccgg
     5701 cgtcaccgac ttggccgccg gcctcggcgt aaaaaggcgt cagggaaaga acggcttcgc
     5761 ccgtttctcc ttccttgatc gcgttcaccc gcttcccctc cttgaagaga gcgaggaccc
     5821 ggctttccgc gacctcgccc ttgtcggctc cgacatattt gaccgaggat ggggaaatcc
     5881 ccgcgtaggc tttcttctct ttcatccggg cgtccccttt ccaggtccgg cgggcccgcg
     5941 tcttctgccg ctccagctcg ttctggaaaa cggcctcgtc gaccgtgatc ccctcctcct
     6001 gggccagctc gcgcgtcaag tccaagggaa aaccgaatgt gtcatacagc ttgaaggcgg
     6061 cctcgccggc gagaacgcgc gatcctgctt ttcgggtctc ccgggcgaat tcctggaaat
     6121 accggaagcc ggacgagagc gttcggctga accgctcctc ctcggcccgg cagatccggg
     6181 agatcagggg cagcgacgat gtcaattccg gataggcgtc cctcatgatg ccggccacga
     6241 cgcccaaaag ggcgtggacg aacggctctt cgaagccgag cttctgcccc tgccggaagg
     6301 cccggcgtat gagtcggcgc aggacatatc cccggccgtc gttggccggc atgatcccgt
     6361 cgccgatgag gaaggcgatc gaccgggcgt gatcggcgat gatccggatg gaaacatcgg
     6421 attcgtctcc ggacggatat tcccggcccg tcttgcggct gacgtcctcg atcaggggca
     6481 tgaacagatc cgtgtcccag ttcgacgttt ttccctgaag gacggccgtg agcctctcta
     6541 atcccatgcc ggtgtcgatg gagggccggg gcaggggccg ccgccccccc ttcccgtcct
     6601 gctcgaactg catgaacaca aggttccaca attcgacgaa ccggtcgctg ccgctctcga
     6661 tcaagtcgcg cggcgcgccc ggttcgagaa gcggggcgaa atcatagtgc aattcggaac
     6721 aagggccgca cggccccgtg tctcccatct cccagaagtt atccgtcgtc ccgaagcgga
     6781 agacgcgatc ggcggggagc ccgatctcct cccgccagat ccggtcggcc tcttcatcat
     6841 cctcgtagac ggtggcgtaa agccggtcct tgtcgatgtg atatacttcg gtgatcagct
     6901 cccaagcgaa ggccacggcc tccttcttga aataatcccc gaaggaaaag ttgccgagca
     6961 tctcgaaaaa cgtgtgatgc ttagccgtcc ggccgacctg gtccaggtcg ttgtgcttgc
     7021 cgctgacccg catgcatttc tggatcgagg cggcccggac atagcttctt ctttccaatc
     7081 cgaggaagac gttcttgaac tgattcatcc cggcgttggc gaacaggagc gtcgggtcgt
     7141 ccttgggcag aagcgaggag ctcgcgacga ctttgtgtcc gcgccgggag aaataatcga
     7201 ggaacgtttc ccggatgtcc tgggcgttca ttccgaatgg ttctcccgtt tggccgcggc
     7261 gttgagatcg cgttccatgt cctcgagcgc cagctcggcg ctggtcgtga tgcccttgac
     7321 ccggagct
//
LOCUS       DAOOXH010000107         7043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_12155_length_7043_cov_6.141099, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000107 DAOOXH010000000
VERSION     DAOOXH010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12155_length_7043_cov_6.141099"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            465..1676
                     /locus_tag="PKV64_10150"
     CDS             465..1676
                     /locus_tag="PKV64_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPN17473.1"
                     /translation="MSFPESLLSQTTRSMGVSFIREMIKATKGVEGMISLAGGLPSPD
                     SFPKEQLASLFKEVIEKEGADVLQYGPSEGDDFLKEQILRFEKEPGVLKEELLITDGA
                     TNGIYFFTRAVIDPGDIVICEGPSFNGTLVAIEACGAELAAVGMDEEGLLVDRVRETI
                     LARRKEGRTVKFIYTIPEFQNPSGRTMSLARRRELIRVARELNVLILEDQPYRDLRFM
                     GRRIDSIWDIARAEFDDARTVTICKSFSKVLGPGLRLGFAAGPADLIAAMAKWAQKVI
                     VSPEGVVQRVVARFLERDLLEPHIEKITAMYRHKREVMISALKETMPPSIRWTEPEGG
                     LFIWLTCPETYDTDVLFHEAAKAGVAYIPGSKFYPSGTEKRNEIRLNFSYATAEDLRT
                     GVARLAKILSR"
     gene            complement(1706..2041)
                     /locus_tag="PKV64_10155"
     CDS             complement(1706..2041)
                     /locus_tag="PKV64_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17474.1"
                     /translation="MKRHERNLLKAMASAPGKPALAELLAFHDKQIAWLQQERLAHLI
                     TTLFFGLFFVLSLGFTMWRFSPAMLALTGMLLVMVTAYVFHYYRLENTVQRWYSISNR
                     IRASLLAQD"
     gene            complement(2207..5725)
                     /gene="nifJ"
                     /locus_tag="PKV64_10160"
     CDS             complement(2207..5725)
                     /gene="nifJ"
                     /locus_tag="PKV64_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPN17475.1"
                     /translation="MKKNFKTIDGNTAATHVSYAYSEVAAIYPITPSSTMGELADEWA
                     AQGKKNIFGQTVDVVEMQSEGGASGAVHGALSAGALTTTFTASQGLMLMLPNMHKIAG
                     EMLPTVFLVSARSLACQALSIFGDHSDVMSARNTGFAMTCAGSVQEAQDLAIVAHLST
                     LRTRIPFLHFFDGFRTSNVIEKVDVVDYETLAGLFEPQYLDDFRKRSLNPERPTMKNG
                     QENPDVYFQGRETINKFYQEAPALVQHYMDKVGKAIGRQYHLFDYFGAPDAEDVIIMM
                     GSGAETAEETINYLNTRGSKLGLIKVRLYRPFDVEALKKALPKSVKRIAVLDRTKEPG
                     SIGEPLYLDVVAALAGSGLKIIGGRYGLSSKDFTPAQVKAVYDHLAGKAFHSFTVGIT
                     DDVTHLSVPVGPVLDTELPGTVRCKFWGYGSDGTVGANKNSIKIIADNTDMYGQGYFQ
                     YDSKKSGGVTISHLRFGKQPIQSQYYLNTVDFIALHKPSYIGRLDILEGIREGGTFLI
                     NSNWNASEVFENLTADMQKTIIDKKIKVYTIDAYKIAGELGLGLRINTIMQACFFKLS
                     GVLPEAEAIALIKKAIEKTYGRKGADVVKMNWAAVDHASDGLAAVPIPDKIGSKAAPM
                     IQIVPDDASAFAKDVIDPVTRFKGDLLPVSKMPFDGVIPTATSCLEKRGIAVEVPAWI
                     PENCIQCNQCSLVCPHAAIRPKQIAPADLKGAPATFKTIKSNSKNDRDLAYRLQVYVE
                     DCVGCENCVEVCPSKVKALKMVPIEDARKAGENENLAFFEKAPYDVLDGNKRDSIKGS
                     QLLRPLFEFSGACSGCGETPYIKLATQLFGDRMIIGNATGCSSIYGGTFPTSPYCQTA
                     EGKGPAWGNSLFEDNAEYGFGMRLAVDANRKLLAASIEAVLAAGTTPALADALAKMKA
                     AWKDTGGEAKAAAAAVRAALPGALKSADGANPHLAKIDELKDFLVEKSVWSFGGDGWA
                     YDIGYGGLDHVLATNRNVNILVLDTEVYSNTGGQASKATPTGSVAKFAASGKKTTKKD
                     LGRMAMTYGYVYVAAVAMGANMNQCIKAFQEAEAYNGPSIIIAYAPCINHGIDMKKTQ
                     AEEKLAVDTGYWILYRYNPLLALEGKNPLQLDSREPKLAYGDFLKNEVRYRTLTQQYP
                     EIAKELFARAAEEAKRRYAVYKKMAE"
     gene            complement(5793..6398)
                     /gene="recR"
                     /locus_tag="PKV64_10165"
     CDS             complement(5793..6398)
                     /gene="recR"
                     /locus_tag="PKV64_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPN17476.1"
                     /translation="MNEYAPPLGRLIEELKRIPGIGSKSAQRMAFHLLAGNQDDAERL
                     ADAIRDLKKNLLSCSICNNITQADPCSYCTDPNRGDECLCVVEEPFHIAAIEKSGVFH
                     GRYYVLLGALAPLKGIGPESLRLEKLEQRLKNGRFKEIIIATNPTSEGEATAHFLVRA
                     LQGYNIKFTRLAMGLPVGSDIDFADQVTVRKALEGRTDLSD"
     gene            complement(6398..6709)
                     /locus_tag="PKV64_10170"
     CDS             complement(6398..6709)
                     /locus_tag="PKV64_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004577797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPN17477.1"
                     /translation="MKGFGDIQKMLKSAKEMQERLQRETAEMRIEGSAGGGMVTVVLD
                     GQKNVLDIGLDPEVVNKDDIDMLKDLILAAFRDGSAKVDAALADKLGGLGAGLKIPGM
                     F"
     gene            complement(6716..>7043)
                     /locus_tag="PKV64_10175"
     CDS             complement(6716..>7043)
                     /locus_tag="PKV64_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17478.1"
                     /translation="SPAKPSALDAAKAFIQRRAAAAAAPPASAKTPAPAPARPARPAA
                     SSDPATEPKAREDASAALRAARRERETGRDAALKNENVRMFQEKLKGRVISVEDPEPP
                     RPKDEE"
BASE COUNT         1241 a         2138 c         2248 g         1416 t
ORIGIN      
        1 cgtcgcgccg ggcctcgatg tccaagccga gggcgtcctc ttgagtccgg ggcaatactc
       61 ccaggacgga tctcgttgca tcgtcagccc catccgggag tcgacgggga ggacggattc
      121 ggcgcttggg gggctcgacg ctgacgcttg ggacgaaaac cgtctttgcg cggccagcga
      181 cggacagtct cccgcccgat ccgaaggcct tccttttccg ccggcatctc gacgaagcgg
      241 cggttaacag cccttgcctc catcgagctt cacatcggct caaagtgatc atctcctttg
      301 tggcagcctc ccctcggaag gggacatttt accttggcag ttaaagggga cattttcgct
      361 ttgctatcac aaatttgcgg cccggcgttt tcccggcggc gcaattcggt tataatattc
      421 tttcgacttc ctcaaatcga aaaccaatca aaggagactt gcccgtgtcc tttccggaat
      481 ccctgctttc ccaaaccacc cggtcgatgg gcgtttcgtt catccgggag atgatcaagg
      541 cgaccaaggg ggtcgagggg atgatctccc tggccggcgg cctgcccagc cccgattcct
      601 tccccaagga acagctcgcc tcgcttttca aggaagtgat cgaaaaggaa ggggccgatg
      661 ttctccagta cggaccgagc gaaggggacg attttctcaa ggagcagatc ctccggttcg
      721 agaaggagcc cggcgtcctt aaggaagagc tcctgattac ggacggcgcg accaacggca
      781 tctatttctt cacccgggcg gtcatcgatc ccggcgacat cgtcatctgc gaggggccgt
      841 cgttcaacgg cacgctcgtc gcgatcgagg cctgcggagc ggagctggcc gccgtgggca
      901 tggacgagga aggcctgctc gtggaccgcg tccgggagac gatcctcgcc cggaggaagg
      961 aaggccggac cgtcaagttc atctacacga tccccgagtt tcaaaatccc agcggccgga
     1021 cgatgagcct ggcccggcgc cgggagctga tccgggtcgc ccgggaattg aatgtcctga
     1081 tcctcgagga ccagccctac cgcgatctcc ggttcatggg ccggcggatc gattcgatct
     1141 gggacatcgc ccgggccgaa ttcgacgacg cccgcaccgt cacgatctgc aagtccttct
     1201 ccaaggtcct cgggcccggg ttgcgtctcg gcttcgccgc cgggccggcc gatctcatcg
     1261 cggccatggc caagtgggcc cagaaggtca tcgtctcgcc cgaaggcgtg gtccagcggg
     1321 tcgtcgcccg gttcctggag cgcgaccttc tcgaacccca catcgagaag atcacggcga
     1381 tgtaccgcca caagcgggaa gtcatgattt ccgccctcaa ggagacgatg cctccatcca
     1441 tccgctggac ggagcccgag ggcggcttgt tcatctggct gacctgcccc gagacatatg
     1501 atacggacgt tcttttccac gaggcggcca aggccggcgt cgcctacatc cccggaagca
     1561 agttctaccc ctcggggacg gaaaaacgga acgaaatccg gcttaatttc tcctacgcga
     1621 cggcggaaga cctgcggacg ggagtcgccc ggctggcgaa gatcctgagc cgctgagcgg
     1681 ccgggcggac gcggagatcc gccggctagt cttgggccag caggctcgcc cggatcctgt
     1741 tggaaatcga ataccaccgc tggaccgtgt tttccagccg gtaataatgg aagacgtaag
     1801 cggttaccat gacgagcagc atccccgtga gggccagcat ggccggcgaa aaccgccaca
     1861 tcgtgaaccc cagggacaag acgaaaaaca atccgaaaaa caacgtcgtg atcaggtgag
     1921 ccaagcgctc ttgttgaagc caagcgatct gtttatcgtg aaaggccaga agctcggcta
     1981 aagccggctt gcccggggcg gacgccatgg ccttcagcag attccgttcg tgtcgtttca
     2041 tatagcgggt catgatgacg aaatcatatt tccggccggg agaacgtgtc aagaagacga
     2101 ttcgggaggg ccggatcccc cgctcgcaaa ataaaacgcc ccgccgctcc cggtcaaggg
     2161 aacggcgggg cgggcgcggt tcgagccggc cttcgccggc gtccttttac tcggccatct
     2221 ttttataaac cgcgtaacgc ctcttggctt cctcggccgc ccgggcaaac agctccttgg
     2281 cgatctcggg atactgctgc gtcaacgtcc ggtagcggac ctcgttcttc aggaagtctc
     2341 cgtaggccag tttcggctcc ctggagtcga gctggagcgg attcttccct tccaaggcca
     2401 ggagcggatt gtaccgatag aggatccagt agcccgtatc gacggccagc ttttcctcgg
     2461 cctgggtctt cttcatgtcg attccgtggt tgatgcacgg agcataggcg atgatgatcg
     2521 acggcccgtt gtacgcctcg gcttcctgga aggccttgat gcactggttc atattggcgc
     2581 ccatggccac cgcggccacg taaacgtagc cgtaggtcat ggccattctg cccaaatcct
     2641 tcttggtcgt cttcttgccc gaagcggcga acttggcgac cgacccggtc ggggtcgcct
     2701 tggacgcctg gccgccggtg ttggaataga cctcggtgtc gagaaccagg atgttgacgt
     2761 tccggttcgt ggccaggacg tggtccaagc cgccgtagcc gatgtcgtag gcccagccgt
     2821 cgccgccgaa ggaccagacg ctcttttcaa ccaggaaatc cttgagctcg tcgatcttgg
     2881 ccaggtgagg attggctccg tcggcgctct tgagcgcgcc gggcaaggcc gcccgaacgg
     2941 ccgccgcggc cgccttggct tcgccgcccg tgtctttcca ggccgctttc atcttggcca
     3001 gggcgtcggc cagggcgggg gtcgttccgg ccgccaggac cgcctcgatc gaggcggcca
     3061 gcagcttccg gttggcgtcc accgccagcc gcatgccgaa tccgtactcg gcgttgtcct
     3121 cgaacaggga gttgccccag gccggcccct tcccttccgc cgtctggcag tagggcgagg
     3181 tcgggaacgt gccgccgtaa atggacgagc agccggtggc gttgccgatg atcatccggt
     3241 caccgaaaag ctgggtggcc agcttgatgt agggcgtctc gccgcagccc gagcaggcgc
     3301 cgctgaattc gaacagcggc cgcaggagct ggctgccctt gatgctgtcg cgcttgttgc
     3361 cgtccaggac gtcataggga gctttctcga agaaggccag gttctcgttt tcccctgcct
     3421 tccgggcgtc ctcgatgggg accatcttga gggctttgac cttgctggga cagacctcga
     3481 cgcagttttc gcagccgacg caatcctcaa cgtaaacttg gaggcggtag gcgagatccc
     3541 ggtcgttctt ggagttggac ttgatcgtct tgaacgtcgc cggagcgcct ttcaaatcgg
     3601 ctggagcgat ctgtttcggc cggatggcgg cgtgggggca gacgagcgaa cactggttgc
     3661 actggatgca attttccggg atccaggccg ggacctcgac cgcgatcccg cgcttttcca
     3721 ggcaggacgt ggccgtggga atgacgccgt caaacggcat cttggagaca gggagcaggt
     3781 cgcccttgaa gcgggtgacc ggatcgatga catccttggc gaacgcggag gcgtcgtcgg
     3841 ggacgatctg gatcatcgga gcggccttgg agccgatctt atccggaatc gggacggccg
     3901 ccaggccgtc ggaggcatgg tccacggcgg cccagttcat tttaacgacg tccgcgcctt
     3961 tccggccgta cgtcttttcg atcgccttct tgatcagggc gatagcctcg gcttcgggga
     4021 gcacgcccga gagcttgaag aagcaggcct gcatgatcgt gttgatccgg aggcccaggc
     4081 cgagctcgcc ggcgatcttg taggcgtcga tggtgtacac tttgatcttc ttgtcgatga
     4141 tcgtcttctg catgtccgcg gtcaggttct cgaagacctc cgaggcgttc cagttggagt
     4201 tgatcaggaa cgttccgccc tcgcggatcc cctcgagaat gtcgagacgg ccgatgtaag
     4261 acggcttgtg gagcgcgatg aaatcgacgg tgttgagata gtactgggac tggatgggct
     4321 gcttgccgaa gcggaggtgg gagatggtca caccgccgga cttcttggag tcgtactgga
     4381 aatacccctg gccgtacatg tcggtgttgt cggcgatgat cttgatcgaa ttcttgttgg
     4441 cgccgacggt gccgtccgag ccgtagcccc agaacttgca gcggaccgtg ccgggaagct
     4501 cggtgtccag aaccgggccg acggggaccg aaagatgggt gacgtcgtcc gtgatcccga
     4561 cggtgaagct gtggaaggct tttccggcca ggtggtcata gacggccttg acctgggccg
     4621 gggtgaaatc cttcgaggac aagccgtagc ggccgccgat gatttttaag ccggagccgg
     4681 cgagggccgc gacaacgtcc aggtaaagcg gctcgccgat ggagcccggt tccttggtcc
     4741 ggtcgaggac ggcgatccgc ttgaccgact tgggcagcgc tttcttgagg gcttccacgt
     4801 cgaaaggccg gtagaggcgg accttgatca gcccgagctt ggagccgcgg gtgttgaggt
     4861 aattgatcgt ttcctcggcc gtttccgcgc ccgaacccat catgatgatg acatcctcgg
     4921 cgtcgggggc tccgaaataa tcgaagagat ggtactgccg gccgatcgcc ttgccgactt
     4981 tatccatgta atgctgaacg agagccgggg cttcttgata gaacttgttg atcgtttccc
     5041 ggccctggaa ataaacgtcc ggattttcct ggccgttctt catcgtcggc cgttccggat
     5101 tgagcgaccg cttccggaaa tcgtcgaggt attgcggctc gaacaagccg gccagggtct
     5161 cataatcgac gacgtcgact ttttcgatga cgttggacgt ccggaatccg tcgaagaaat
     5221 gaagaaacgg aatccgggtc cggagagtcg aaaggtgggc gacgatggcc aggtcctggg
     5281 cttcctggac cgagccggcg caggtcatgg cgaagccggt gttgcgggcc gacatgacgt
     5341 cgctgtgatc gccgaagatc gagagcgcct ggcaggccag ggagcgggcc gagaccagga
     5401 agaccgtggg gagcatctcc ccggcgatct tgtgcatgtt cgggagcatg agcatcaagc
     5461 cttgggaggc ggtgaacgtc gtcgtcaggg cgccggccga aagggccccg tggaccgcgc
     5521 ccgaggcgcc cccttccgac tgcatttcga ccacgtccac cgtctggccg aagatgttct
     5581 tcttgccctg ggccgcccat tcatcggcca actcgcccat cgtcgaggag ggcgtgatcg
     5641 gataaatggc ggcgacttcg gaatacgcgt aggaaacatg ggtcgcggcc gtgtttccgt
     5701 cgatcgtttt gaagtttttc ttcatgaacc aagctccttt gccgccgcgc gaaaacggcg
     5761 ttttcgttta taactcgcgg aatatccgct gtttaatccg aaagatccgt ccgcccttcc
     5821 aaggccttgc ggacggtgac ttggtcggcg aaatcgatgt ccgacccgac cggcaatccc
     5881 atggccaggc gcgtgaattt aatattatac ccctgaaggg ccctcacaag gaagtgggcc
     5941 gtcgcctccc cttccgaggt cgggttggtg gcgatgatga tctccttgaa acggccgttt
     6001 ttcaagcgct gctcgagctt ttcaagacgg aggctctccg gcccgatccc cttgagcggg
     6061 gccagggcgc ccaggaggac gtaataccgt ccatggaaga cgccggattt ctcgatcgcc
     6121 gcgatatgga agggctcttc gacgacgcac aagcactcgt cgccgcggtt cggatcggtg
     6181 caataggaac acggatcggc ctgggtgata ttgttgcaga tcgagcagga cagcaggttt
     6241 ttcttcaggt cgcggattgc atcagccagg cgctcggcgt cgtcctggtt gccggccaga
     6301 aggtgaaagg ccatccgctg ggcgctcttg ctgccgatgc cggggatcct cttcagctcc
     6361 tcgatgaggc gtccgagagg aggggcgtat tcgttcatca gaacatcccg gggattttca
     6421 ggcccgcgcc caatccgccg agcttgtcgg ccagggccgc gtcgaccttg gccgagccgt
     6481 ctcggaacgc cgccaggatc agatccttga gcatgtcgat gtcgtccttg ttgacgactt
     6541 ccggatccag cccgatatcg aggacgtttt tctgtccgtc cagaacgacc gtcaccattc
     6601 cgccgccggc cgagccctcg atccgcatct ccgccgtctc ccgctggagg cgctcctgca
     6661 tttccttggc cgacttcagc attttctgga tgtcgccgaa acccttcatc tttcctcact
     6721 cctcgtcttt cggacgcggc ggctcgggat cttccacgga aatgacccgg cccttgagct
     6781 tttcctggaa catccggacg ttttcattct tgagcgcggc atcccggcct gtttcgcgct
     6841 cgcggcgggc cgcgcgcagg gcggccgacg cgtcctcgcg ggctttcggc tcggtggccg
     6901 gatcgctcga ggcggccggg cgcgccggcc gcgccggagc cggagccggt gttttcgcgg
     6961 aagccggggg cgccgccgcg gcggccgcgc gccgctggat gaaggccttg gcggcgtcca
     7021 gggccgacgg cttcgccgga gag
//
LOCUS       DAOOXH010000108         7031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_12187_length_7031_cov_6.465453, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000108 DAOOXH010000000
VERSION     DAOOXH010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12187_length_7031_cov_6.465453"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PKV64_10180"
     CDS             <1..289
                     /locus_tag="PKV64_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17479.1"
                     /translation="LKIPWGESLVRVRFPSPALEIPLNNRLNCGWAEAAFHFSEPKTP
                     AKRQIPLPPAKKQEEITDKIFSFREQANAPRAQAAEASAGARKQAERAVME"
     gene            299..649
                     /locus_tag="PKV64_10185"
     CDS             299..649
                     /locus_tag="PKV64_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009069910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HPN17480.1"
                     /translation="MTKTIIKLKRIYEKPEKADGFRVLVDRLWPRGISKERARLDLWL
                     KEVAPSTELRQWFGHDPKKWTEFKKRYRRELSKNPAFEELRSAAEKHKSVTLLFGAKD
                     EERNEAAVLKEDIE"
     gene            complement(690..1631)
                     /locus_tag="PKV64_10190"
     CDS             complement(690..1631)
                     /locus_tag="PKV64_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HPN17481.1"
                     /translation="MKIALLAEIKNVLPRIDLLPAIEEGFAAYSEGRAVVPPVGELIL
                     DKGEVHIKYGAIAGEDFYVIKIASGFYGNPALGLPSGNGCLLVFEQKTGVPAAVLLDE
                     GYLTDIRTAAAGAVAAKYCAPRTVERIGVAGAGVQGRLQLEWLKRVTVCRDALVWGPL
                     PDELEGYRREMKSRGFSVETTREAAAIPRTCNLIVTATPSKTPLLSAADLRPGTHITA
                     VGSDTPDKQELDSEILARADVVVADSLAQCRLRGEIHKALAAGKIDESRVVELGNIIS
                     GRASGRTSDSQITVADLTGVAVQDIKIASAVYRALAP"
     gene            complement(1672..2808)
                     /locus_tag="PKV64_10195"
     CDS             complement(1672..2808)
                     /locus_tag="PKV64_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPN17482.1"
                     /translation="MPRFQPFVMERMMSKFEKAVDYNLSESGVHPLTLNELFQDDPEG
                     MARLMSLEMDYAHANGIPDLRRNIAALYPGAGPENVLVTVGAIEANYDILWSLLSPGD
                     EIVVMLPNYMQIWGLAKNLGFSLKTFSLDEKNGWAPDLDGLKKAVTRKTKLIAVCNPN
                     NPTGYILDKKEMEAVVETARGAGAWILADEVYAGAERTAEEQTPTFYGLYDKVLAVGS
                     LSKAYGLPGLRVGWAVGPAEIIDELWARHEYLTLSATMLSNHLAAYALSPAVRPRLIN
                     RGRGFIRRGYPVLERWMGEHEGILSAVPPRAAAIAFVKYNLDIPSTRFVERLREEKKV
                     LIVPGDHFGLDHFVRISYGLPHEYLTPALGRIHELIAETGAIKR"
     gene            complement(2887..5388)
                     /locus_tag="PKV64_10200"
     CDS             complement(2887..5388)
                     /locus_tag="PKV64_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPN17483.1"
                     /translation="MKATAIGLAALVLWAVSPSISPADADAGGSPWILTPEEYLDSPG
                     VSVLVFHDIYPEGKQGGVEIIQHGERVAAVGDVRLEETPGQWSRRPETGKREIDRERC
                     SIRVPAAFAGSGPAYAVRVEPAGPGFRVAVDLDKPLPAALEGRVSFNLELFPSAYIGK
                     TYHLGRTAAVFPRQGNGPMIPGGFGTVRPAALASGPKLSLAPEDPLRMMTIESTGADI
                     ELYDGRNTADNGWFVLRTLLRSGCTRNAAEWIITPHRIPGWIRDPVIAVSQAGYHPDQ
                     EKRAVIELDRNAGPPGEAVLCRIEAGGGPAEVLRGPVVPWNGRFLRYDYATFDFTGVR
                     DPGMYVLKYGSRSGPPFRIGRDVYRRDVWQPTLETYLPVQMCHVEVRDVDRIWHGACH
                     LDDAVQAPPSTVHFDSYEQGPVLPYAFPARGRIPHLDRGGWHDAGDFDLAAGSQAFVT
                     WMLVLIRETFGLATDQTTVRPDERLVLLHRPDGIPDILQQIRHGAENLLSGYRAAGHS
                     FGGIIEGSIIQYSLIGDTASMTDNRIYDPSLGRTEAREGRSGRPDDRWAFTDPDTSLE
                     YQVAAALAAASRGLREIDAGLALECLETALLAWDREQGRTPVSARAAYIPGRPEIQEI
                     LASVELLLTTKEERFRRRLEALGPVIEANIAETGWAVARAFPLLGEGPLRERTARALE
                     KYSADTARELAANPFGVLWRPRIWGVGWDLQEFAVQRYFLNQALPKLFDREGIFRVLH
                     FILGCHPGSNVSFVSGVGAKSLTIGYGMNRADWSYIPGMNVSGTALIRPDFPELKDDF
                     PFLWQQAENVIGGAATYIFTVLAADRLLNGPEENK"
     gene            complement(5474..6541)
                     /locus_tag="PKV64_10205"
     CDS             complement(5474..6541)
                     /locus_tag="PKV64_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17484.1"
                     /translation="MTRLRRRIVSALVLLGLAAVPAPARPQDKTAGLFQKGIAFTGYA
                     KDAYDGGGARQALRELAAANAEWVQILVTGYQATRRSTVIDRTGEETPADASLAAIIG
                     FAKELGLKVFLKPHIDLREEPAGWRGEIGQGFTEADWAAWFASYREFIVHYAAMAEAL
                     GAEMFSAGCELDRTVGREADWRATIAAVRAAYRGLLTYADDQAESDPEAVRWWDAVDL
                     IGLDAYPTLTDNPHPSVRDFRAGWNRYLQSLHEISRRWGKNIVVTEIGYRSIQGGAQD
                     PWNWQKEGPVDLEVQANAYQAGLQALLGRGWIQGVYWWQWMPDPENGGPKDTGYSPHG
                     KPAERVLAAFYRDAAPVHPNF"
     gene            complement(6578..>7031)
                     /locus_tag="PKV64_10210"
     CDS             complement(6578..>7031)
                     /locus_tag="PKV64_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HPN17485.1"
                     /translation="EPFADLIRGGTALVIEDAPAADVGSIGSYNILEWKSRGCVGVVT
                     SAAARDTDEITAERVPLYFQRPGRGIRPGRNEIESVNRPIVCGGVLVRPGDIIVADGD
                     GVIVVPRAAAAEVAAYARKILDGDKEGRRDLYKKLGLQPDDSIKKPPR"
BASE COUNT         1234 a         2278 c         2316 g         1203 t
ORIGIN      
        1 acttaaaatc ccttggggcg aaagcctcgt acgggttcga ttcccgtccc cggcacttga
       61 aatcccccta aataaccgtt tgaactgcgg atgggcggag gcggcgtttc atttctcgga
      121 gccaaaaacg cctgcaaaac ggcaaattcc attgccgcca gcgaaaaaac aagaggaaat
      181 taccgataaa atattctctt ttcgcgagca agcgaacgct ccgcgcgcgc aagcggccga
      241 agcttcggcg ggcgcgcgga aacaagccga gcgggcggtt atggagtaaa attaatccat
      301 gacgaagaca ataatcaaac tcaaacgaat ttacgaaaaa ccggagaaag ccgacggctt
      361 ccgagtgctg gtggaccgtc tttggccgcg gggcatttca aaggagcggg cgcggctgga
      421 cttgtggctg aaggaggtcg cgccgtcaac ggaattgcgg caatggtttg gccacgaccc
      481 gaaaaaatgg acggagttta aaaagagata ccgccgggag ctgtcgaaaa atccggcgtt
      541 tgaggaattg agaagcgcgg ccgaaaagca caagtccgtc acgcttcttt ttggagccaa
      601 agacgaggag cgcaacgagg cggcggttct caaagaggat attgaataac ccccgccggc
      661 gcgaaaggcg agaccgcggc caagaagaat cagggggcga gggcgcgata aacggccgag
      721 gcgatcttga tatcctggac ggccactccg gtgaggtcgg cgacggtgat ttggctgtcc
      781 gatgtccgcc ccgaggcccg gccgctgatg atattcccga gctcgacgac ccggctttcg
      841 tcgatttttc ccgccgccag agccttgtgg atctcgcccc ggagccggca ttgggccagg
      901 ctgtcggcca ccacgacgtc ggcccgggcg aggatttcgg agtcgagctc ctgtttgtcc
      961 ggcgtgtccg agccgacggc ggtgatgtgc gtccccggcc ggaggtcggc cgcagaaaga
     1021 agcggcgtct tcgacggcgt cgcggtgacg atcaggttgc aggtccgcgg gatcgcggcc
     1081 gcttcccggg tcgtctccac cgaaaaaccg cgcgatttca tctccctccg atagccctcg
     1141 agctcgtcgg gaagcggtcc ccaaaccagc gcgtcccggc agacggtgac gcgtttgagc
     1201 cattcgagct gcagccggcc ttgaaccccg gcgccggcga cgccgatgcg ctcgaccgtc
     1261 cgcggagcgc agtacttggc cgcgaccgct ccggccgccg cggtccggat gtcggtcaaa
     1321 tatccctcgt cgagcaggac ggcggccggg acgccggttt tctgctcgaa gacgagcagg
     1381 cagccgttgc ccgacggcag gcccaaggcg ggattgccgt agaagcccga ggcgatcttg
     1441 atcacgtaga agtcctcgcc ggcgatggcg ccgtacttga tatggacttc gcccttgtcc
     1501 aggatcagct cgccgacggg cggaacaacc gcccggccct cggaataggc ggcgaaccct
     1561 tcctcgatgg ccgggagaag atcgatccgc ggaaggacgt tcttgatctc ggccagaaga
     1621 gcgattttca cggagcctcc agggagagca gccggcgccg ccatgcgcct tctatcgttt
     1681 gatcgcgccc gtttccgcga tcaattcgtg gatgcgcccc agggccggcg tcaggtactc
     1741 gtggggcagg ccgtagctga tgcggacgaa atgatccagg ccgaagtggt cgcccggcac
     1801 gatgagaacc tttttctcct cgcgcagccg ctcgacgaat cgcgtcgagg gaatatccaa
     1861 attatatttc acgaaagcga tggccgccgc ccgcggaggc acggccgaca ggatcccctc
     1921 gtgttcgccc atccaccgct ccaggacggg atatccccgg cggatgaagc cccggccccg
     1981 attgatgagg cggggccgga cggcggggga gagggcgtag gcggccaggt gattggagag
     2041 catcgtcgcg ctcagggtca agtactcgtg ccgggcccaa agctcgtcga tgatttcggc
     2101 cgggccgacg gcccagccga cccgaaggcc cggcaggccg taggccttgc tcaggctgcc
     2161 cacggccagg accttgtcgt ataagccgta gaaggtgggc gtttgttctt cggccgtccg
     2221 ctcggctccg gcgtagacct cgtcggccag gatccaggcg cccgcgcccc gggccgtttc
     2281 gacgacggct tccatctctt ttttatcgag gatatagccg gtcggattgt tgggattgca
     2341 gacggcgatc aatttcgtct tccgggtcac ggcctttttc aagccgtcga ggtcgggcgc
     2401 ccagccgttt ttttcgtcca aggaaaaggt tttgagggag aatcccaggt tcttggccag
     2461 cccccagatc tgcatgtagt tgggcagcat gacgacgatc tcgtcgccgg gtgaaagcag
     2521 gctccagagg atgtcgtaat tggcctcgat ggctcccacg gtcacgagga cgttttccgg
     2581 cccggcgccg ggatagagag cggcgatgtt ccgccgcaga tcgggaatgc cgttggcgtg
     2641 ggcgtagtcc atttcgagcg acatcagccg ggccattccc tccgggtcgt cctggaagag
     2701 ctcgttcagg gtcaacgggt ggacgccgct ctcggagagg ttatagtcga ccgctttctc
     2761 gaatttggac atcatccgtt ccatcacgaa aggctgaaac cggggcatgt tcgacctcct
     2821 tagacgcggt gaggataagc cgtgacgcgg cccgccgtca agacattaaa caggtgccgt
     2881 gtctccctat ttgttttcct ccggaccgtt gagcagacgg tccgcggcca gaacggtgaa
     2941 gatgtacgtc gccgctccgc cgatgacgtt ttcggcctgc tgccagagaa acggaaaatc
     3001 gtccttgagc tccgggaaat ccggccggat gagggccgtc ccggaaacgt tcatccccgg
     3061 gatgtacgac cagtcggccc ggttcattcc gtagccgatc gtcagggact tggccccgac
     3121 gcccgagacg aacgagacgt tcgagccggg atggcagcct aaaataaaat gaaggacgcg
     3181 gaagattccc tcgcggtcga agagcttggg aagcgcctga ttcaaaaaat atcgctggac
     3241 ggcgaattcc tgcaaatccc agcccacgcc ccagatccgc ggccgccaga ggacgccgaa
     3301 gggattggcg gcgagctcgc gggcggtgtc cgccgagtat ttctccaaag cccgcgccgt
     3361 cctttcacgg agcggcccct ccccgagcag gggaaaggcc cgggcgacgg cccagccggt
     3421 ctcggcgatg ttcgcctcga tcacgggacc cagggcttcc agccgccgtc ggaaccgttc
     3481 ctcctttgtg gtcaacagga gctcgaccga ggccaggatt tcctggattt ccgggcggcc
     3541 ggggatgtaa gcggcccggg ccgagaccgg cgtccggcct tgttcccggt cccaggccag
     3601 gagagccgtt tccaagcact ccagggcgag gccggcatcg atctcccgaa ggccgcggga
     3661 ggcggccgcc agggcggcgg cgacttgata ctccaacgac gtgtccggat ccgtgaaggc
     3721 ccaccggtcg tccggccgcc ccgagcgccc ttccctggct tcggtccggc cgagggaggg
     3781 atcgtaaatc cggttgtccg tcatcgaggc ggtgtccccg atcaggctgt attggatgat
     3841 cgagccctcg atgattccgc caaagctgtg tccggccgcc cggtagcccg agagcaggtt
     3901 ctcagccccg tgcctgattt gctgaaggat gtcgggaatc ccgtccggcc ggtggagaag
     3961 gacgaggcgc tcgtccggac ggaccgtcgt ctggtcggtc gccaggccga acgtctcccg
     4021 gatcagaacg agcatccagg tcacgaacgc ttgagagccg gcggccaagt cgaagtcccc
     4081 ggcgtcgtgc cagccgcccc ggtcgagatg ggggatgcgg ccgcgcgccg ggaaagcgta
     4141 cggcaggacg ggtccctgct cgtagctgtc gaaatgaacc gtcgacggcg gcgcctggac
     4201 ggcatcgtcc aagtgacagg ccccgtgcca tatgcgatcg acgtcccgga cctccacgtg
     4261 gcacatctgg acgggaaggt aggtctcgag cgtcggctgc cagacgtccc ggcgatagac
     4321 gtcccggccg atccggaacg gcggcccgga ccgggatccg tatttcagga cgtacatccc
     4381 gggatcccga accccggtga agtcgaacgt cgcgtaatcg tagcggagga atctcccgtt
     4441 ccaggggacg accggcccgc ggaggacttc ggccggcccg cctcccgcct cgatccggca
     4501 caggacggct tcccccggcg ggccggcgtt ccggtcgagc tcgatcaccg cccgtttctc
     4561 ctggtcggga tggtagccgg cctgggaaac ggcgatgacc ggatcccgaa tccagccggg
     4621 gatccggtgg ggcgtgatga tccactcggc cgcgttccgc gtgcaaccgg aacggaggag
     4681 ggtgcgaagg acgaaccagc cgttatcggc cgtgttgcgg ccgtcataga gctcgatgtc
     4741 cgcgcccgtc gattcgatgg tcatcatccg gaggggatcc tcgggggcga gcgacagttt
     4801 cggaccggag gccagggcgg ccggccggac cgtcccgaat ccgcccggga tcatgggacc
     4861 gtttccctgc cggggaaaaa cggccgcggt ccggccgaga tgataggttt tcccgatata
     4921 agccgacggg aaaagctcca ggttgaagct gacccggccc tcgagggcgg ccggcagcgg
     4981 tttgtccaga tcgacggcga cgcggaaccc gggacccgcc ggctcgaccc gcacggcata
     5041 ggccgggccg gacccggcga aagcggccgg cacccggatc gaacaccgct cccggtcgat
     5101 ctcccgcttg cccgtttcgg gccgccggct ccattgtccc ggcgtttctt cgaggcggac
     5161 gtctcccacg gctgccaccc gttcgccgtg ctggatgatt tccactccgc cctgcttgcc
     5221 ttccggataa atgtcgtgga agaccaggac ggagactccc ggggaatcca ggtattcctc
     5281 gggagtgaga atccagggag atcctcccgc gtccgcgtcc gccggggaaa tggaagggga
     5341 gaccgcccag agaaccaagg ccgcaagccc gatcgccgtc gctttcattt tgcctccttc
     5401 ccgccgccgg gtggcgccgt cccgggtgaa aaacggggca cggctctctc tcggcccgcg
     5461 ccctcccgga aattcagaaa ttagggtgaa ccggagccgc gtcccggtaa aacgccgcca
     5521 gaacccgctc cgccggcttg ccgtgggggc tgtagcccgt gtctttcggt ccgccgtttt
     5581 ccgggtccgg catccactgc caccagtaga cgccctgaat ccagccgcgg ccgagcaagg
     5641 cctgcaggcc ggcctggtag gcgttggcct ggacctcgag atccaccggg ccctccttct
     5701 gccagttcca aggatcctgg gccccgccct ggatgctccg gtagccgatc tcggtcacga
     5761 cgatgttttt cccccatcgc ctggaaattt cgtgaagcga ttgaagatag cggttccatc
     5821 cggcccgaaa atcccgcacg gagggatgcg ggttgtcggt caaggtcggg tacgcgtcca
     5881 ggccgatgag atccacggcg tcccaccagc ggacggcctc gggatcgctt tcggcctggt
     5941 cgtccgcgta cgtcaaaagg ccgcggtagg cggcccggac ggcggcgatc gtcgcccgcc
     6001 aatccgcttc ccggccgacg gtccggtcca gctcgcagcc ggcgctgaac atctccgcgc
     6061 cgagagcttc ggccatcgcc gcgtagtgga cgatgaattc ccggtacgag gcgaaccagg
     6121 cggcccagtc cgcctcggtg aatccttggc cgatctcgcc ccgccacccg gcgggctctt
     6181 cccgcaggtc gatgtgcggc ttgagaaaaa ccttgaggcc gagctccttg gcgaagccga
     6241 tgattgcggc cagggatgcg tcggcggggg tttcctctcc cgtccggtcg atgaccgtcg
     6301 accggcgcgt ggcctggtat ccggtgacca ggatctggac ccattcggcg ttcgccgccg
     6361 ccagctcccg cagggcttgg cgggcacctc cgccgtcgta ggcatccttc gcgtaaccgg
     6421 tgaaggcgat ccccttctgg aaaaggccgg ccgttttgtc ctgcggccgg gccggggccg
     6481 gcaccgccgc cagccccaga agaaccaggg ccgaaacgat ccgccgccgg agacgcgtca
     6541 tccgccttct cccttcggtc cgccggccgg aatccgctca ccggggcggt tttttgatcg
     6601 aatcatccgg ctgaaggccg agctttttat agagatcccg ccgtccttcc ttgtccccgt
     6661 ccaggatttt ccgggcgtag gcggcgacct cggccgcggc ggcccgggga acgacgatga
     6721 ccccgtcccc gtcggcgaca atgatgtcgc ccggccggac caggacgccg ccgcagacga
     6781 tcggccggtt caccgactcg atctcgtttc gccccggccg gatcccgcgt cccggccgtt
     6841 ggaaataaag ggggacgcgc tcggccgtga tctcgtcggt gtcgcgggcc gcggcgctcg
     6901 tgacgacgcc cacgcagccg cggcttttcc attccaagat gttgtaggat ccgatcgagc
     6961 cgacatcggc cgccggggcg tcctcgatca ccagggccgt ccccccgcgg atcaggtcgg
     7021 cgaagggctc g
//
LOCUS       DAOOXH010000109         7028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_12193_length_7028_cov_8.115015, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000109 DAOOXH010000000
VERSION     DAOOXH010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12193_length_7028_cov_8.115015"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PKV64_10215"
     CDS             complement(<1..292)
                     /locus_tag="PKV64_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HPN17486.1"
                     /translation="MPFPIGFDNEKYLAEQEAAILDRVRRFENKLYLEFGGKILFDMH
                     ASRVLPGFDPNVKMRLLQKLRDRADILLCIYAGDIERKKVRADFGITYDVDAL"
     gene            794..1558
                     /locus_tag="PKV64_10220"
     CDS             794..1558
                     /locus_tag="PKV64_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPN17487.1"
                     /translation="MSGAIHLKPFEVRAVDIDQSGRIQPVVFLDYLLESASEQAARFD
                     IGVTDLFKKGRTWVLSRLHVEFDRFPAWGESLTIETWPSAKLPLYAIRDFAVFNDRGP
                     AARATSSWLIVDLETRRPVRMAEHLDDFPLDGRRAVEDDFAPLPSPAREDARKEFPVF
                     FSDLDINRHFTATAYIHRALETVPREILFELRPVSIEVNFRAEAFYGDVIVSRLERIE
                     AAGGEAGPRFLHRLSRADGDKELTLLRTSWARPPQA"
     gene            1575..2441
                     /locus_tag="PKV64_10225"
     CDS             1575..2441
                     /locus_tag="PKV64_10225"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HPN17488.1"
                     /translation="MIISASRRTDVPAFFAREFMDGVRAGFLNVPHPRNPRLVSRVEL
                     SPSGVEAIVFWTRDARPLLPRLRELDDRGYAYYFQYTLLDGPTVLDPEGPRGEEAVAA
                     FRALSERLGPDRVVWRYDPIVFSNRTGAAFHRRRFERLASALAGATKRVMISLVDFYP
                     SAAARFRELERRGVRVRPPSPEDLAGLMPALVETAAGRELEILSCAEEINLEPWGVRP
                     GKCIDDGLLSRLLGRPLRLRKDPRQRKACGCVESRDIGVYGTCPRGCVYCYAAGRRRP
                     AASTAGETDRLR"
     gene            2798..4948
                     /locus_tag="PKV64_10230"
     CDS             2798..4948
                     /locus_tag="PKV64_10230"
                     /inference="COORDINATES: protein motif:HMM:NF020928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HPN17489.1"
                     /translation="MKIAERLAAEFKIGTAAVARTLALLDAGAAIPFIARYRKEQTGG
                     LAEHRIREIARRRDGLRTLEERRTALTAALREKGLLSPERETALQETTDAAELEDFAL
                     SLTPRENSRAARGRDAGLEPLARWLVDLEDAAADVSAEAAKFINAGKGIETAEAALRV
                     AGDILAEEWADDPEARKSHRALVRAEGVVVTSAREEYAGRKSKYEIYYLFRESIQAIP
                     SHRALAVIRGEREKVLKIELAFPKKPALRNLVVRFIRHPKSAAAPFLTDVALDALDRI
                     LAPAVKAGVLREIREKAEGEAFKVFGENLRDLLMAAPAGRQAVLGVDPGFRAGCKLAV
                     VAPTGKFIEYRTIYPNAPKNDADGAKRALLEAVCEHKIALVAVGGGQAGRETETFVRA
                     ALEELPERSRPACVVVGETAAGVYAVSDAAAAEFPRLDVAVRRAISLARRLQDPLAEL
                     VKIDPRSIGVGQYQNDVDQAALKRSLEEVVESCVNAVGADVNLASEDQLKYISGLNRA
                     TAAAVAAHRRERGPFRTREALKEVKGIGEKTFEQAAGFLRIPGAADPRDRSAIHPERY
                     AFVEELAAKAGTTVAGLFEDEALRKTIESRSCVTPDVGLPTVRDILKELEAPGRDPRP
                     AFRTVVFSETAKTVDDLAPEQILEGVVSNVVDFGAFVDIGVRQDGLVHISEMGDKFIS
                     DPHKILKVGQLVKVRVLSVDRERKRIALSLKKIP"
     gene            complement(4956..6275)
                     /locus_tag="PKV64_10235"
     CDS             complement(4956..6275)
                     /locus_tag="PKV64_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPN17490.1"
                     /translation="MNADRRMLPPDSEPGLAVRPAELAGLDPSPSAMAVVPVNRMFEI
                     RDALAVYGRDFPGGEKFDASQGDGGASLPGVPSEIYEAAHRLQVEHGSAYDQPFGCAA
                     FRRSVVEDYWQLDPGSSWGPENVLAVQGGRDGLLKAYEAALFLGRGRRGDFVITSRVP
                     WISYSWGPYLIGANVLLAPGSEEEAWRITPEGVRTCASYVRRFDGREIALLVITSPDN
                     PTGRVLGLEDQAELARAAFSAGVPFVLFDWIYHQVTDGEPGDLNRFLRLFDPVERERC
                     LFLDGLTKSLGASNVRGAHLVASKNIVKFIQNRASHAIVPSFYSQAVAMAAYRMGYAR
                     ACAGVIGPTNQSRGILSAFLSANGFRSIIGKGYYAFIDVGPWIDRAGFKDSADVGTYL
                     AERFGLAVVPGVYFSVFGGRWIRFSYALPPDKTARAATRLKEGLASL"
     gene            complement(6272..>7028)
                     /locus_tag="PKV64_10240"
     CDS             complement(6272..>7028)
                     /locus_tag="PKV64_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17491.1"
                     /translation="RAAQVIAVYIGVESPSADALAAAGKKHNLGDPPARQIEAFLDHG
                     IFPYLSMILGLDGEDRGAGRRLREFLGACRSPFLMLNLINPVAGSRLRERAAAAGRLL
                     DHPLYGEYNLIPMKTDRPYAEVIKDYAETLRWFYSDRRLLGFCRDLEHHAGRVQDRRG
                     RDAFAAGLSKVFLLKLLFLYLGLCLKSGSWSGLVQVVKAAGKSRSEALFSLAIRSVAL
                     GAKDAYRKTGRRALKGLIRLRTAELYPYERKRP"
BASE COUNT         1195 a         2397 c         2294 g         1142 t
ORIGIN      
        1 tcaaggcgtc gacgtcgtag gtgatgccga aatccgcccg gacctttttc ctctcgatgt
       61 cgccggcata gatgcacagg aggatgtccg cccggtcgcg gagcttttgg agcaggcgca
      121 tcttgacatt gggatcgaac ccgggaagaa cccgggaagc gtgcatgtca aagagaatct
      181 tgccgccgaa ttccagatac agcttgtttt cgaagcggcg gactcgatca agaatcgccg
      241 cttcctgttc ggccaggtac ttttcgttgt cgaatccgat cgggaagggc atgaagaaac
      301 tcctcggccg cggcgtcccg gtgacaggcc gccgcggccg atttctttta tagactttgt
      361 cggcgcgcct gtcaaacgga ggcatcggcc tccctgggga gcgaccctcg gccgacccct
      421 ccgaagggcg ggagacatcc ccgatactcc ggtgagacgg gaagcccgcc ggaatgtcca
      481 ggtcgttcgc gaggcccgtt ccgttttttt cggcggcgtt tcccccgccc gaccgaagga
      541 ctctccgcct ctatgcccgg acgctctttt gatttcgtcc cgccggcctg gtcgggctcg
      601 gacgggaaca ccgcccccgc cgcctcggct tttccgcggt tccgctctct ccgcgcccgg
      661 cttccgggga cgggaaagaa tacgatcaag gggagtctca tccatgattc cggatctgaa
      721 cgtcggacgc ggccggcgta ggtttgcgcc ggacggcggg gtttcctata ctgggattcg
      781 acggaggtgt tcgatgagcg gcgcgattca cctgaagcca ttcgaggtcc gggcggtgga
      841 catcgatcag agcgggcgca ttcagcccgt cgtcttcctc gattatctcc ttgagtccgc
      901 ctccgaacag gccgcccggt tcgacatcgg cgtcacggac ctgttcaaaa aaggccggac
      961 ctgggttctc tcccggcttc acgtcgagtt cgaccggttt cccgcctggg gggaatcgct
     1021 cacgatcgag acgtggccct cggccaagct gcccctctac gcgatccgcg atttcgcggt
     1081 cttcaacgac cgcggcccgg ccgcccgggc gacgtcctcc tggctgatcg tcgatttgga
     1141 aacccggcgg ccggttcgga tggccgagca cttggacgac tttccgctgg acggccgcag
     1201 ggcggtcgag gacgatttcg ccccccttcc ctccccggcc cgcgaggacg cccggaagga
     1261 attcccggtc tttttctcgg acctggacat caaccgccat ttcacggcca cggcctacat
     1321 ccaccgggcc ctggagaccg ttccccggga gatcctcttc gagctccggc cggtctcgat
     1381 cgaggtcaat ttccgggcgg aagcctttta cggcgacgtc atcgtttccc gcctcgagcg
     1441 gatcgaggcc gccggcggcg aggccggccc gcgctttctt caccgccttt cccgggccga
     1501 tggagataag gagctgaccc tcctccggac gtcctgggcc cggccgccgc aagcctgaca
     1561 cccccccgga agccatgatc atcagcgcca gccgccggac ggatgtcccg gccttcttcg
     1621 cccgggaatt catggacggc gtccgggccg gcttcctcaa cgtcccccat ccgcggaatc
     1681 cccgcctcgt ctcccgggtg gagttgagcc cctccggtgt cgaggcgatc gtcttctgga
     1741 cgcgcgacgc ccgccccctc cttccccgtc tccgggagct cgacgaccgg ggatacgcct
     1801 attacttcca gtatacgctc ctggacggcc cgaccgtcct agatcccgag ggcccccggg
     1861 gggaggaggc ggtcgccgcg ttccgcgcgc tgtccgaacg cctcggcccc gaccgggtgg
     1921 tctggcgcta tgacccgatc gtcttttcca accggaccgg ggcggctttt catcgccggc
     1981 ggttcgaacg cctcgcctcg gccctggccg gggcgacgaa acgggtgatg atcagcctgg
     2041 tcgacttcta cccctcggcc gccgcccgtt tccgcgaatt ggaacggagg ggggtgcgcg
     2101 tccgtccgcc ttccccggag gatctcgccg ggctgatgcc ggccctggtc gaaaccgccg
     2161 ccggccggga acttgaaatc ctgagctgcg ccgaggagat caatcttgag ccttggggcg
     2221 tccggccggg gaaatgcatc gacgacggcc ttctttcccg cctgctcggc cggcctctcc
     2281 gcctccgcaa ggatccgcgt cagcggaagg cctgcggctg cgtcgagagc cgggacatcg
     2341 gcgtttatgg aacgtgcccg cgcggctgcg tctactgcta cgccgccggc cggaggcgcc
     2401 ccgccgcctc cacggccgga gaaacggatc gacttcggtg aacgacttgc gccgggaccg
     2461 gcagaggcaa cggctgtcgc gcaaccgcgg ccggatccgc cgaccgaatg ccttgccgtg
     2521 ataaatcagc cgtggccggc gtggccgggg ccggtcgtgc cgtcgagggc tcatccggcc
     2581 gcatcggggc ccagggcggg cggctctcct gaaaatgaag agcgccttcc cggccggaaa
     2641 cctcaatccc cgcgaatccg cgccccttaa ttcacgccgc gcttcccccc gctttcaccg
     2701 ccctgttcat cgtcgagcca tcaccgcttt gacaggcgga ccccgggtat gggatatttc
     2761 ttttcttgag aatcgacgtc cccggaagga gtctgtcgtg aaaatcgccg aacgcctcgc
     2821 cgccgaattc aagatcggga ccgccgcggt cgcccgaacc ctggcccttc tggacgcggg
     2881 ggcggccatc cccttcatcg cccggtaccg gaaagagcag acgggcggct tggccgaaca
     2941 ccgcatccgg gagatcgccc ggcgccgcga cggcctccgg acactggagg agcggaggac
     3001 cgccctgacg gcggccctgc gggaaaaagg gcttctctcg cccgaacgcg aaaccgccct
     3061 ccaggaaacg acggacgccg ccgagctgga ggacttcgcc ctgtccctca ctcccaggga
     3121 aaattcgcgg gccgcccggg gccgggacgc cggtcttgaa ccgctggccc ggtggctggt
     3181 cgatctcgag gacgcggcgg ccgacgtgag cgccgaagcg gcgaaattca tcaacgccgg
     3241 caagggaatc gaaaccgccg aggcggccct gcgggtcgcc ggcgacatcc tggccgagga
     3301 atgggccgac gatcctgaag cccgcaagtc tcaccgggcc ctggtccggg ccgagggcgt
     3361 cgtcgtcacc tcggcccgcg aggagtacgc cggccggaaa tccaaatacg agatctatta
     3421 tctcttccgg gaaagcatcc aggcgattcc ttcccaccgg gccctggccg tcatccgggg
     3481 cgaacgggaa aaggtcctca agatcgagct ggccttcccc aagaagccgg ctctccgcaa
     3541 tctcgtcgtc cggttcatcc gccatccgaa aagcgccgcc gctccgttcc tgaccgacgt
     3601 cgccctggac gcgctcgacc ggatcctggc cccggccgtc aaggcgggcg tccttcggga
     3661 aatccgggag aaagccgagg gggaagcctt caaggtcttc ggcgaaaacc tccgagatct
     3721 tctgatggcc gccccggccg ggcggcaggc cgtgctgggc gtcgatccgg ggttccgggc
     3781 cggctgcaag ctggcggtcg tcgccccgac aggcaaattc atcgagtacc ggacgattta
     3841 tccgaacgcc ccgaaaaacg acgcggacgg cgcgaagcgg gctctcttgg aggcggtctg
     3901 cgagcacaag atcgccctgg tcgccgtcgg cggagggcag gccggccggg agacggaaac
     3961 gttcgtccgg gcggccctcg aggagctgcc ggagcggagc cggccggcgt gcgtggtcgt
     4021 cggagaaacc gccgccggcg tctacgccgt ctccgacgcg gccgcggccg aattcccccg
     4081 cctggatgtg gctgtccgcc gggcgatttc gctcgcccgt cgcctccagg atccgctggc
     4141 cgagctggtc aagatcgatc cccgctccat cggcgtcggc caataccaaa acgacgtcga
     4201 ccaagccgcc ttgaagcgat ccttggaaga ggtcgtcgaa agctgcgtca acgcggtcgg
     4261 ggcggacgtc aacttggcct ccgaagatca attgaaatac atctccggtc tgaaccgggc
     4321 gacggccgcg gccgtcgccg ctcatcgccg cgagcgcggc cccttccgaa cccgcgaggc
     4381 cctgaaggaa gtcaagggca tcggcgaaaa aacattcgaa caggccgccg ggttcctccg
     4441 catccccgga gcggccgatc cccgggaccg ttcggccatc caccccgagc gctatgcctt
     4501 cgtcgaggag ctggcggcca aggccggcac gaccgtcgcc ggcctcttcg aggacgaggc
     4561 cttgaggaaa acgatcgaat cccgctcctg cgtcaccccc gacgtcgggc tcccgacggt
     4621 ccgggacatc ctcaaggaac tggaagcccc gggacgcgac ccgcggcccg cgttccgcac
     4681 ggtcgttttt tccgaaacgg ccaaaaccgt cgacgacctc gccccggaac agatcctcga
     4741 gggcgtggtc tcaaacgtcg tcgacttcgg agccttcgtc gacatcggcg tccgccagga
     4801 cggcctggtc cacatctccg agatgggcga taaattcatt tccgacccgc acaagatcct
     4861 caaggtcggc cagctcgtca aggtccgggt cctgagcgtg gaccgggagc ggaagcggat
     4921 cgccctctcc ttgaaaaaaa tcccctgacc gggaattaca aggaggccaa cccctccttg
     4981 aggcgggtgg cggcccgggc ggtcttatcc ggaggcaagg cgtaggaaaa acggatccac
     5041 cggccgccga agacgctgaa atagactccc ggaaccacgg ccaggccgaa gcgctcggct
     5101 agatacgtgc ccacatcggc cgaatccttg aagcccgccc ggtcgatcca agggccgaca
     5161 tcgatgaaag cgtaataccc cttgccgatg atcgaccgga atccgttggc cgacaggaac
     5221 gcggaaagaa tcccccggct ttggttggtc gggccgataa cgcccgcgca agcccgggcg
     5281 taccccatcc ggtaggcggc catggccacg gcctggctgt aaaaagaggg cacgatcgcg
     5341 tgcgaggccc ggttctggat gaatttcacg atattcttcg aagcgacgag atgggcgccg
     5401 cggacgttgc tcgctccgag cgacttggtg aggccgtcca ggaagaggca gcgctcgcgt
     5461 tcgacgggat cgaacaggcg gaggaagcgg ttgaggtcgc ccggctcgcc gtcggtcacc
     5521 tggtggtaga tccagtcgaa gagaacgaag ggaacgccgg cggaaaacgc cgcccgggcc
     5581 agctccgcct ggtcctccag gcccaggacg cgtcccgtgg gattgtcggg gctggtgatg
     5641 acgagcaggg cgatttcccg gccgtcgaac cggcggacgt aagaggcgca ggttcgcacg
     5701 ccttccggcg tgatccgcca ggcctcctcc tcgcttcccg gcgccaggag gacgttggct
     5761 ccgatgagat aaggccccca gctgtaggaa atccagggga cccgggaggt gatcacgaaa
     5821 tcccctctcc gcccgcggcc caaaaaaagc gccgcctcat aggctttgag caggccgtcc
     5881 cggccgccct ggacggcgag gacgttttcc ggtccccagg acgatccggg atcgagctgc
     5941 caataatcct cgaccacgga gcggcggaag gcggcgcagc cgaacggctg gtcgtaggcg
     6001 ctgccgtgct cgacctggag ccggtgagcc gcctcgtaaa tctcggacgg aacgccgggg
     6061 agactggctc ccccgtcgcc ctggctggcg tcgaatttct caccgcccgg gaaatccctc
     6121 ccgtaaaccg ccagggcgtc gcggatctcg aacatccggt taaccggcac gacggccatg
     6181 gccgacggcg acggatcgag cccggccaac tcggccggac ggacggccaa accgggctcg
     6241 gaatcggggg gaagcattcg cctgtcggcg ttcatggccg tttcctttca tacggatata
     6301 gctccgcggt ccggaggcgg atcaggcctt tcaaagcccg ccggccggtc ttccggtagg
     6361 cgtctttcgc gccgagggcc acgctccgga tcgccaggga aaagagcgcc tcggaccggg
     6421 attttccggc ggccttgacg acttggacga ggccggacca ggatcccgac ttcaggcaga
     6481 ggccgaggta aaggaagagc agcttcagga ggaatacctt ggaaaggccg gcggcgaagg
     6541 cgtcccgccc ccgccggtcc tggacgcggc cggcgtgatg ctcgagatcg cggcaaaagc
     6601 cgagaaggcg ccggtcggaa taaaaccagc ggagggtctc cgcgtaatcc ttgatgactt
     6661 cggcataggg ccggtcggtt ttcatcggga tgaggttgta ttcgccgtag aggggatggt
     6721 cgaggagccg gcccgcggcc gcggctcgtt cccggagtct cgatccggcc accgggttga
     6781 tcaggttcag catcaggaac ggcgaccggc aggcgcccag gaattcgcgg agacggcggc
     6841 cggcgccgcg gtcttctcca tccaggccga gaatcatgga aaggtaaggg aaaattccgt
     6901 ggtcgaggaa cgcctcgatt tgccgggccg gcggatcccc gagattatgc tttttgccgg
     6961 cggccgccaa ggcgtccgcc gacgggcttt ccaccccgat ataaacggcg atcacctggg
     7021 ccgcccgc
//
LOCUS       DAOOXH010000110         6818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_12705_length_6818_cov_6.964513, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000110 DAOOXH010000000
VERSION     DAOOXH010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12705_length_6818_cov_6.964513"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..1476
                     /locus_tag="PKV64_10245"
     CDS             268..1476
                     /locus_tag="PKV64_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPN17492.1"
                     /translation="MSSYICRIADDAGTVFRRTFPASTASDCRRHFESEGFCVLSVRR
                     ERRKIGSLLRGSEGKIKARDFIMFNQEFTAMIRAGFPVLRCLETIGGRTKNPALKNVL
                     GRVENDIRHGKALSEAFAPFEDRFSKVYTAALMAGEISGTLPETIGQYVSYAKTIDRT
                     ARRVRAALTYPTLIICFSAGLLLLLINFVLPNFSEFYADFETSLPYLTSALIQFSLFL
                     RRNWIIWPVLAAALVVLAWRMKRNPDMRRRWDRLKLRVPVARSLIVESAVSLFSRTLS
                     LLLGAGASLVDAVALASQAVPNKHLAGRMEPVPAGIRNGEAFSEALSKTNFFPPMALD
                     LVRIGESSANLSGMLRELADVTEERIQQKLDTLVSLVEPVVIILTGVLVAGMLLAVYL
                     PIFRIIQVAR"
     gene            1481..3091
                     /locus_tag="PKV64_10250"
     CDS             1481..3091
                     /locus_tag="PKV64_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPN17493.1"
                     /translation="MTPSPSRPSAPADSEAVRRLADRCGVPFTDLADVRIDRDLVQSF
                     PPEFLYRANVIPLEDDGANLRVAVADPSDLETIDAIRTLSGRNVVVEAAPPRAIQEAL
                     RKSETALQVLKGATEGFIQQLVEKEGGEEEEVISLEKLADQDGSIIKLVNTIIFTAVQ
                     KRASDIHIESKDDNVHVKYRIDGVLGEAMEPIDKKFQAPIISRIKVMSDLDIAERRVP
                     QDGRFRLKIKDKTIDFRVSIMPSIYGEDSVVRILDKEMITEAISELKLDLLGFSDDVL
                     RRFRRYIAQPYGMVLVTGPTGSGKTTTLYAALTEIRSVEDKIVTIEDPVEYQVDGITQ
                     IPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDPETAQIAIQSALTGHLVFTTVHANN
                     VFDVIGRFLHMQVDPYSFISALNCILAQRLVRLLCSKCKTPVRYPDEALRESGLDPER
                     YRERVFYEAGGCAECGFTGYHGRTAIAEILDLSDTVREMILIRKPLSEVRKRAKAEGM
                     VSIRDVGVGKVLSGLTSLKELNKVTFVE"
     gene            3095..>3900
                     /locus_tag="PKV64_10255"
     CDS             3095..>3900
                     /locus_tag="PKV64_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17494.1"
                     /translation="MAAFIDSWIRRLTAPDIPAAVFHVARGSLGGLRRARQSASVKSS
                     VVRSLPAGLVEPSFDKPNLPRPEEFQSAVKDAVRRIGSPEGPVALLLPETSVKTVLLS
                     FDSLPPAPDEREKIIRWRLARTLPLPPAETRLSFAVSRTNGRARAFCVLAAAGVVREY
                     EKAFAAAGLDVRTVGLPTPALLGCLPKNGPANQVIVNIEEDYFAALAILDGGPALFRV
                     KPLSTGNLWEEAAEETAATLRFLEDREKKEIESVWLRPAAGGDAGTGAAL"
     assembly_gap    3901..4000
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4161..4724
                     /locus_tag="PKV64_10260"
     CDS             4161..4724
                     /locus_tag="PKV64_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17495.1"
                     /translation="MNGGLRLPINLATRPLRNRRLFRAAVGIPVVLFLLFGGGAGVLL
                     LHSTARLRADERTRAELERRIQSAGRERADKTGQSQTMRKRDSELVDVVNGVIVRKNF
                     SWVGLFSRLEQALPPQCSIVSLSPLQLTETGLRVNMKVITPGLPGLLVLIENLTARGF
                     KDVVLRDELTGGGRLICEIGFDYNGTL"
     gene            4711..5265
                     /locus_tag="PKV64_10265"
     CDS             4711..5265
                     /locus_tag="PKV64_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17496.1"
                     /translation="MELFDLLDEGERRIVSIAAVVCAAVFAVLLVFGVRAWLNAGRAS
                     ARRAAVETEWRAADQARKAAVAEWSRWDGASADVRELRGTWLYDRGRGIQTMRSDLAD
                     VLKKAGASVPEITYGESEVVKNRMRRYTVGFHWAGSYPAFRRLLETLEAHPRALFVSK
                     VDFRVIVEGHLEAGVTLEGYAVDE"
     gene            5258..>5658
                     /locus_tag="PKV64_10270"
     CDS             5258..>5658
                     /locus_tag="PKV64_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17497.1"
                     /translation="MNKPDRRGIVFVAAVLCLGALAAAGPDAPPPLIRRDLLRKEAPV
                     FPKIVRDPFSTVVFIVDSGESGEPSDLTGKELSPAETPPPPPPPVVRYIGFARYAAPG
                     PPAAILMINERPWALAEGETAPNGWTALKIT"
     assembly_gap    5659..5758
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5759..>6818
                     /locus_tag="PKV64_10275"
     CDS             <5759..>6818
                     /locus_tag="PKV64_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HPN17498.1"
                     /translation="SAETSLRAVLQSLGRAGRVNIIFDEAFRDVPFQTDFTNVTFEQA
                     LGTVCQATKNFYRIIDERTVIIVPDQPAKRLQYEVSCIRTFYLSNAQAQEMTASLTNM
                     LRGAAKIPLIFFDKDLNSVTIRDTPQVIELAEKLIRIWDKPKGEVVIDMELLEVSRTR
                     LRQLGLSLDQNKVGLRYGAPLAENSSSAGWEQLSKIDLSKSENYFVNLPVAYLQFLET
                     DSDTKIVAQPRLRGLADEEIHQLVGEKVPVPKTTWNPIAAGGYSQQPVTSYDYQDVGI
                     DITFTPRVHLEKEVTLAMELKITSLGGKGYADLPIINTRELKGTLRLRDGETTMFAGL
                     LRDAERKSLSGIPFLKNLP"
BASE COUNT         1161 a         2203 c         2066 g         1188 t
ORIGIN      
        1 gcccggcggc gtaataccgt ccggcccagg cgcggaggcc gagcaggaga aggcggtcga
       61 aggccgcgaa cgaaacgagg aagacgagga ccgccgggac ggccgccgcc cggcgtttcc
      121 gggggacgga ggagaaggac atgacggagg gaagtatatc gggccgtttt ccggccgtca
      181 aaccggacgg caaattgaca cggtcggccg tttgccctaa tctagtaaac ggtttccccc
      241 tagcggaaag ggcgggatcg aaacgccatg tcttcctata tttgccggat cgccgacgac
      301 gccgggacgg tcttccgccg gacttttccg gcttccaccg cctcggactg ccgccggcat
      361 ttcgagtcgg aaggattttg cgtcctgtcc gtccgccgcg aacggcggaa gatcggaagc
      421 cttctgcggg gatccgaggg gaaaatcaag gcccgcgatt tcatcatgtt caaccaggaa
      481 ttcacggcca tgatccgggc cggattcccg gtgctccgct gcctggagac gatcggcggc
      541 cggacgaaga atcccgccct gaagaacgtt ctgggcaggg tggaaaacga catccgccac
      601 ggcaaagcct tgtccgaggc cttcgctccg ttcgaggacc gcttctcgaa ggtttacacg
      661 gcggccttga tggccgggga aatcagcgga accctgcccg agacgatcgg ccaatacgtc
      721 agctatgcca agaccatcga ccggacggcc cgccgggtcc gcgcggccct gacgtatccg
      781 accttgatca tctgtttttc cgcgggcctg ctgcttcttt tgatcaattt cgtcctgccc
      841 aatttttccg agttttacgc tgatttcgaa acgagccttc cgtacctgac ttcggccctc
      901 atccagtttt ccctttttct ccgccggaac tggatcatct ggccggttct ggccgcggcc
      961 ctcgtcgtcc tcgcctggcg gatgaagcgg aatccggaca tgagacgccg ctgggaccgg
     1021 ctgaagctcc gggtgcctgt ggctcggtcc ctgatcgtcg aatcggcggt ctccctcttc
     1081 agccggacgc tgagccttct cctgggcgcg ggagcttcgc tcgtagacgc cgtcgccctg
     1141 gcctcccaag ccgtccccaa caagcacctg gccggccgga tggagcccgt gcccgccggc
     1201 atccgcaacg gcgaggcgtt ttccgaggcc ttgagcaaaa cgaatttttt ccccccgatg
     1261 gccctggatc tcgtccgcat cggagaatcc tcggccaacc tcagcgggat gctccgcgag
     1321 ctggccgacg tcaccgagga gcggatccaa cagaagctcg acacgctcgt ctcgctcgtc
     1381 gagcccgtcg tcatcattct caccggcgtg ctggtggccg ggatgctcct ggccgtttat
     1441 ctccccattt tccgaatcat tcaagtggcc cggtgatccg atgaccccct cgccttcccg
     1501 tccctccgct ccggccgatt ccgaagccgt ccgtcggctc gccgatcgct gcggcgtccc
     1561 cttcaccgac ctggccgacg tccggatcga ccgggacctg gttcaatcgt tccccccgga
     1621 attcctctac cgggccaacg tcatccccct cgaggacgac ggggcgaatc tccgcgtcgc
     1681 cgtcgccgat ccctccgacc tggagacgat cgacgccatc cggaccctct ccggcaggaa
     1741 tgtcgtggtc gaagccgcgc ctccccgggc cattcaggag gccctccgca agagcgaaac
     1801 ggccctccag gttctcaagg gagcgaccga gggctttatc cagcagctcg tggaaaagga
     1861 aggcggggag gaggaggagg tcatctccct ggagaagctg gccgaccagg acggctcgat
     1921 catcaagctg gtcaacacga tcatcttcac ggccgtccag aagcgggctt ccgacatcca
     1981 tatcgaatcc aaggacgaca acgtccacgt caagtaccgg atcgacggcg tcctgggcga
     2041 ggccatggag cccatcgaca agaaattcca ggcgccgatc atctcccgaa tcaaggtcat
     2101 gtccgacctg gacatcgccg agcgccgcgt cccccaagac ggccggttcc gtctgaagat
     2161 caaggacaag acgatcgatt tccgcgtctc gatcatgccc tccatctacg gcgaggattc
     2221 ggtggtccgg atcctcgaca aggaaatgat cacggaggcc atctccgagc tcaagctcga
     2281 cctgctcggc ttctccgacg acgtcctccg gcgcttccgg cgctacatcg cccagccgta
     2341 cggcatggtc ctggtgaccg ggccgacggg aagcggcaag acgacgacct tgtacgccgc
     2401 cttgaccgag atccggtcgg tcgaggacaa gatcgtcacc atcgaggacc cggtcgaata
     2461 ccaggtcgac ggcatcaccc agattccggt caatgagaag aaggggctga cattcgcccg
     2521 gggcttgcgc tcgatcctcc ggcatgatcc cgataaaatc atggtggggg agatccgcga
     2581 tccggagacg gcccaaatcg ccatccagtc cgccctgacc ggccacctcg ttttcacgac
     2641 cgtccatgcc aacaacgtct tcgacgtcat cggccgcttc cttcacatgc aggtcgatcc
     2701 gtacagcttc atctccgccc tgaactgcat tctggcccag cggctggtcc ggctgctctg
     2761 ttccaagtgc aagacgcccg tccgctaccc ggacgaggcg ctgcgggaat ccggcctcga
     2821 cccggaacgg taccgggagc gcgtgtttta tgaggccggc gggtgcgcgg agtgcggatt
     2881 cacaggttac cacggccgga cggccatcgc cgagatcctc gacctctcgg acacggtccg
     2941 ggagatgatc ctgatccgca agcctttgtc cgaggtccgc aagagagcca aggcggaggg
     3001 aatggtttcc atccgggacg tcggcgtcgg aaaggtcctg tccggtttga ccagcctcaa
     3061 ggaattgaat aaagtcacgt ttgtggaata agccatggcc gcgtttattg attcctggat
     3121 caggcgccta acggccccgg acattcccgc ggccgtcttc catgtcgccc gtggatccct
     3181 cggcggtctg cgccgcgccc gccaatccgc gtcggtgaaa agctccgtcg tccggagcct
     3241 tccggccggt ctcgtggagc cgtcgttcga caagccgaat cttccccggc cggaggagtt
     3301 tcaatcggcg gtcaaggacg ccgtccgccg gatcgggtcg ccggaaggac cggtcgccct
     3361 cctccttccg gaaacgtccg ttaaaaccgt cctcctgagc ttcgactcgc tgccgcccgc
     3421 tcccgacgag cgggaaaaga tcatccgctg gcggctggcc cggacgctcc cgctgccccc
     3481 ggccgagacg cggttgtcgt tcgccgtcag ccggacaaac ggccgggccc gggcgttttg
     3541 cgtcctggcc gccgccggag tcgtccggga atacgaaaag gcgttcgccg cggccgggct
     3601 ggatgtccga acagtcggcc ttccgacgcc ggccctgctc ggctgcctgc cgaaaaacgg
     3661 accggcgaac caggtaatcg tcaacatcga ggaggattat ttcgccgccc tggcgattct
     3721 cgacggcgga ccggcgctgt tccgggtgaa gcccctctcg acggggaatt tatgggaaga
     3781 agcggccgag gaaaccgcgg ccaccctccg tttcctcgag gaccgggaaa agaaggaaat
     3841 cgaaagcgtc tggctgcggc cggccgcggg cggggacgcc gggacgggcg cggccctcat
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcgtctggct gcggccggcc
     4021 gcgggcgggg acgccgggac gggcgcggcc ctcatccaaa gccgcgtttc ccgtcccgtc
     4081 cggattctgc cgggcgacgg atcgacgggg attccgccgg ccgaccggat ccgcctggcc
     4141 ccccttcttg gacagatctc atgaacggcg gcctgcgcct tccgatcaac ctggccaccc
     4201 ggccgctgcg gaaccggcgc ttgttccggg cggcggtcgg gattccggtg gtcctttttc
     4261 ttctcttcgg cgggggggcg ggggtccttc tcctccattc gaccgcccgc ctgagggccg
     4321 acgagcggac ccgggccgag ctcgagcgcc gcatccaatc ggccggacgc gaacgagcgg
     4381 ataaaaccgg gcagagccag acgatgagga aacgggattc cgagctcgtc gacgtggtca
     4441 acggcgtcat cgtccggaag aatttttcct gggtggggct tttctcccgc ttggaacaag
     4501 ccctgccgcc gcagtgctcg atcgtctcgc tcagcccgct tcagctgacc gaaacagggc
     4561 ttcgggtcaa catgaaagtc attactccgg gcctgcccgg ccttctggtc ttgattgaaa
     4621 acctgacggc ccggggtttc aaggacgtgg ttctgcggga tgagctgacg ggcggcggcc
     4681 ggctcatctg cgaaatcgga tttgattata atggaactct ttgacctctt ggacgaaggc
     4741 gagcgccgga tcgtttccat cgcggccgtc gtctgcgccg cggtgttcgc cgttcttctc
     4801 gtgttcgggg tcagggcttg gctgaacgcc ggccgggcgt ccgcccggcg ggcggcggtc
     4861 gagacggagt ggcgggcggc cgaccaggcc cggaaggccg ccgtcgccga atggagccgt
     4921 tgggacgggg cctcggccga cgtccgggag ctccgcggga cgtggctgta cgaccggggg
     4981 aggggaatcc agacgatgcg ctccgacctg gccgatgttt taaaaaaggc gggcgcgtcg
     5041 gtcccggaaa tcacgtacgg ggaatccgag gtggtcaaga accggatgcg gcggtacacc
     5101 gtcggcttcc attgggccgg ttcttatccc gctttccgcc ggctcttgga aaccctcgag
     5161 gcccatcccc gggcgctgtt cgtctccaag gtggatttcc gggtcatcgt cgagggacac
     5221 ttggaagccg gcgtcacgct ggaaggatat gccgtcgatg aataagcctg accggcgggg
     5281 aatcgtgttt gtcgccgccg tgctctgcct cggggccctg gcggcggccg ggccggatgc
     5341 ccccccgccc ctgatccgcc gggatcttct ccgaaaagaa gccccggttt ttccgaagat
     5401 cgtccgcgat ccgttttcga cggtcgtctt catcgtcgac tccggcgaaa gcggggagcc
     5461 ttcggatttg acggggaagg agctgtcccc ggctgagacg ccgccgcctc cccctccccc
     5521 ggtcgtccgc tacatcggat tcgcccggta cgccgctccc ggcccgccgg cggccatcct
     5581 catgatcaac gagcggccct gggccctggc cgaaggcgaa accgccccca acggctggac
     5641 ggccctcaag atcacggann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
     5761 tcggcggaaa cgtccctgcg ggcggtcctc caatccctgg gccgggccgg gcgggtcaac
     5821 atcattttcg atgaggcgtt ccgggacgtc ccctttcaga ccgatttcac gaatgtgacc
     5881 ttcgaacaag ccctgggaac agtctgccag gcgaccaaga atttctaccg gatcatcgac
     5941 gagcggacgg tcatcatcgt ccccgaccag ccggccaagc ggcttcaata tgaagtgagc
     6001 tgcatccgga cgttctactt gtccaatgcc caggcccaag agatgaccgc ctccctgacc
     6061 aacatgctcc gcggcgcggc caagatcccc ttgatcttct tcgacaagga tctcaacagc
     6121 gtcacgatcc gggacacgcc tcaggtcatt gagctggccg aaaagctcat ccggatctgg
     6181 gacaagccca agggcgaggt cgtcatcgac atggagcttt tggaggtttc ccggacgcgg
     6241 ctccgtcagc tgggcttgtc gctcgaccag aataaagtcg gcttgagata cggggcgccg
     6301 ctggccgaga attcgtcgtc cgccggctgg gagcagctct cgaagatcga tttatccaag
     6361 agcgaaaatt atttcgtgaa cctgccggtc gcctatctcc agtttctcga gaccgattcg
     6421 gacacaaaga tcgtcgccca gccgcgcctg cgggggctgg ccgacgagga aatccaccag
     6481 ctcgtcgggg agaaggttcc cgtccccaag acgacgtgga atcccatcgc cgccggcggc
     6541 tacagccagc agccggtgac gtcctacgat taccaggacg tcgggatcga catcacgttc
     6601 accccccggg ttcatctgga gaaggaagtg accctggcca tggagctcaa gattacctcg
     6661 ctcgggggaa agggatacgc cgatctgccg atcatcaaca cccgcgagct caagggaacg
     6721 ctccgcctgc gggacggcga gacgacgatg ttcgccggcc tcctccgcga cgcggagcgg
     6781 aaatccctct ccggcatccc cttcctgaag aaccttcc
//
LOCUS       DAOOXH010000111         6770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_12833_length_6770_cov_6.338645, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000111 DAOOXH010000000
VERSION     DAOOXH010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12833_length_6770_cov_6.338645"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PKV64_10280"
     CDS             <1..577
                     /locus_tag="PKV64_10280"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17499.1"
                     /translation="PMKFNRLRNPLGAFVLADSRQKKDTTGMLGFIKSHVLEGADRVR
                     KAIPSFTLHSPRTPEVLAEIEKLPEDHRRLLLGTLTTRDVTARGAALFQAPAFDETAF
                     GRLLNEQHAVLRDLLQTSTPKIEEMIAAALDAGALGAKINGSGGGGCMFAYAPSNPEK
                     VAAAVAAAGGRPFVVRVDEGVRRDDRKGTKE"
     gene            574..1137
                     /gene="efp"
                     /locus_tag="PKV64_10285"
     CDS             574..1137
                     /gene="efp"
                     /locus_tag="PKV64_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPN17500.1"
                     /translation="MIDATQIRKNMILRMDDGQLWRCVEMQLITPGRWKAMVQTKLRS
                     LRDNSVKDHRFRSIDRVEQVHLDEVQMEYLYASGDDYVFMNHETFDQIHLSAEAMGDA
                     MKYLIPNAVFTIEMFEGKAINVVAPITMVLTVAETEPNIKGATASASFKPALMENGLT
                     IMVPPFVLAGEKIKVDTREDKYLERVK"
     gene            complement(1147..3327)
                     /locus_tag="PKV64_10290"
     CDS             complement(1147..3327)
                     /locus_tag="PKV64_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17501.1"
                     /translation="MKRARLLLGAVLLLAAAAAAVAPRQWTLRTFDDFLRGKSEGIAI
                     EADGGLALAPPEDKIDGPSEDFYLSWALDSDGTGYLGTGHGGKIYRLGKDGKAELYFQ
                     TSEMDVTCLLTGPKGVLYAGTSPNGRIYKITAPGKGQELFNPEERYIWDLVLSPAGSL
                     LAAVGESGGIYEISPEGSGRPVFKSDQNHILCLKLGSDGALTAGSGGAGLVYRLEKNG
                     GKTVVVFETPFEEVRAAAFDPEGNLYVSAGGTIKAPRGEAVSAPAAVPGGVDVSIAVS
                     AAAQAAVPAGPAAARPAVVAGLKEPGAVYRIGRDGLARRIWFSPEEQVYTLFWNEAEG
                     KLYFGTGPKGRLYTLDRREKAALLLQSEAEQAYACVPDGPRFYLLSNNPAGLSRISPG
                     RRLSGEYQGPVWDANLTSQWGRIEWEAVVPEGGALQLQSRSGNTAEPGPTWSDWSPPC
                     RNADGEPILSPKGRYLQVRALFKSTSAESGPVLSKLNVNFLQANAAPEVTRLEILGPH
                     EVYLKPPDMNEAIWGLESRLPDAGTKPDEIRMIAAKKTERRGFRTIVWDGQDENGDVL
                     EYAVALRKDGDTNWRTIEDRWAEEIFAFNTVHYPDGIYQVKVTASDALSNPEGMEKTG
                     ERTAGPLLIDNAAPEIRGLEVVRAEGRLTVSFTAVDAFSPVTDARVLLRPGDWRVVFP
                     EDGIADSKSESYKLRLAIPNGADGLVTILVRDAAGNAAAVQRAF"
     gene            complement(3338..5158)
                     /locus_tag="PKV64_10295"
     CDS             complement(3338..5158)
                     /locus_tag="PKV64_10295"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HPN17502.1"
                     /translation="MRKTAVLAATALAVILGAGLARSATEILPLEDVKTGMKGKGRTV
                     FAGNKVEEFDVEILGVLANTGPKRNAIIARLSGRNLEQTGVLQGMSGSPVYIDGKIIG
                     AVAFSFSFAKEPIAGITPIGEMLAIAGDSSGKPGPGTARLPFSERVSLRDLFEAHRDY
                     FSSPAPFEADGRTSAALPVPLLFSGFSPRLLDEIRPYFAGLGFSVRAASAPGAGQRTG
                     IALNTDLNVKEGDPLAVQLISGDLDVSAIGTATYIDGARVYAFGHPLYNLGPVEYGMA
                     KADIITVVPAYNSSFKLGSVGSLIGAVVQDRTSGAYGEIGRVPKTVPLNITLTRESGP
                     AREYRLKLASDKLLTPLLANMAVASLIGAEDRAVGDLSLELSGDVYLDTSPGQAVHIE
                     DLFTGNLGRATTDLSGLVTAVVYFLTNNEFKDVGIHRIDLNIRTSERPKSAVLERVWL
                     DRYEAAPGETLRLKLFIKSYRGESRVEEIPFLAPNLPPGSEFNLIVADTRSLRQVELG
                     QYRTSGIVPRSLEQLVRLLNNLRKNNRVYFKIVAARPGLFLRGEEMPNLTPAMKSLFA
                     SPRAAATAPVEISVSTLREYQMPVPFVFEGLAVIPLKIRN"
     gene            5246..6217
                     /locus_tag="PKV64_10300"
     CDS             5246..6217
                     /locus_tag="PKV64_10300"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPN17503.1"
                     /translation="MRSILITGATGFIGGHLAERLRSRGEAVTALVRDSARGRFLESL
                     GVRLLQGELDAGPPLPENLDVVFHLAGLTRARRKEEYYIVNEKGTASLLDGLPRGERR
                     PRFVYLSTTAAGGPSLPDRPRREDDPARPVSEYGRSKLLAEEEVLARRDRLPVSVVRV
                     GPVYGPRDPGFLDYFKYVRRGFLLSFGREPRPMSVCYIDDLVDGLLAAAEPAAGTGEV
                     FNIGDPTPRTFEDLGLAAAGLLGVRARRIILPFPVIRTAVFFAEAVRPFTRTVGIANR
                     DKYKEYREKAWVADMTKAKARLGFEASWSLEKGLEKTIAWYRDTGRL"
     gene            complement(6234..>6770)
                     /locus_tag="PKV64_10305"
     CDS             complement(6234..>6770)
                     /locus_tag="PKV64_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17504.1"
                     /translation="RGGLLNCPAGASLMRKSGYLAIAAAALVLTGGILRAAAEDLIKV
                     EAGIAPRRLARGQEGKVVLKISLRKGIAVSALPAFKIEVEEGPDLVFPKNFFTGSDLD
                     LAHVEIDGRDCLDLQGPIEIPFTVGQKARRGVYVLRGRVKYFAFDRVGGWCLKSSAKF
                     STAYSTWAIPAKAAASGD"
BASE COUNT         1183 a         2254 c         2195 g         1138 t
ORIGIN      
        1 gccgatgaaa ttcaaccggc tccgcaatcc cctcggggcc ttcgtcctgg ccgattcccg
       61 gcagaaaaaa gacacgaccg gcatgctcgg cttcatcaag agccatgtcc tggagggagc
      121 cgaccgggtc cggaaggcga ttccgtcctt caccctccac tccccccgga cgcccgaggt
      181 cctggccgag atcgaaaagc tgccggagga tcaccgccgc ctgcttctcg gcacgctcac
      241 gacccgcgac gtgacggccc ggggagcggc ccttttccag gcccccgcgt ttgacgaaac
      301 cgccttcggc cggctgttga acgaacagca cgccgttctc cgggacctcc tccaaacgtc
      361 cactcctaaa atcgaggaaa tgatcgcggc cgctcttgac gccggggcgc tcggtgctaa
      421 aataaacggc tccggcggcg ggggctgcat gttcgcctac gccccctcca atcccgaaaa
      481 ggtcgccgcg gccgtcgccg ccgccggcgg acggccgttc gtcgtccggg tggacgaggg
      541 cgtccggcgg gacgacagga aagggacaaa ggaatgatcg acgcgactca aatccgcaaa
      601 aacatgattc tccggatgga cgacggacag ctctggcgct gcgtcgaaat gcagctcatc
      661 accccgggcc ggtggaaagc gatggttcag acgaagctcc ggagcctccg cgacaattcg
      721 gtcaaggacc accgcttccg gtcgatcgac cgggtcgagc aggtccatct cgatgaagtc
      781 cagatggagt atctctacgc ctcgggcgac gattacgtct tcatgaatca cgagacgttc
      841 gatcagatcc acctctcggc cgaagccatg ggcgatgcca tgaagtatct gattcccaac
      901 gccgtcttca cgatcgagat gttcgagggc aaggccatca acgtcgtcgc ccccatcacc
      961 atggtcttga cggtggccga gaccgagccg aacatcaagg gcgcgaccgc ctcggcatcc
     1021 ttcaagccgg cgctcatgga gaacggcctg acgatcatgg tcccaccgtt cgtcctggcc
     1081 ggggaaaaga tcaaagtcga cacgcgcgag gacaagtacc tcgagcgcgt gaagtaagga
     1141 atcccctcaa aacgcccgct ggacggcggc cgcgttgccg gccgcgtcgc ggacgaggat
     1201 cgtcaccagc ccgtcggccc cgttcgggat cgcgaggcgg agcttgtagg actcggattt
     1261 cgagtcggcg atcccgtcct cgggaaaaac cacccgccaa tcgcccggcc ggagaagaac
     1321 ccgggcgtcc gtgaccggcg agaacgcgtc cacggccgtg aagctcacgg tcaagcggcc
     1381 ctcggcccgg acgacctcga ggccgcggat ctccggggcg gcgttgtcga tcagcagggg
     1441 cccggccgtc cgctcccccg ttttctccat cccctcggga ttggaaaggg cgtccgaggc
     1501 ggtcactttc acctggtaga ttccatcggg atagtgaacc gtgttgaagg cgaagatctc
     1561 ctcggcccag cggtcctcga tcgtccgcca gttcgtgtcg ccgtctttcc tcagggccac
     1621 ggcgtattcc aagacgtccc cgttttcgtc ctgtccgtcc cagacgatgg tccgaaagcc
     1681 ccgccgctcc gtttttttgg cggcgatcat ccggatctcg tccggcttcg ttccggcgtc
     1741 gggcaagcgg ctctccaagc cccaaatcgc ctcgttcatg tccggcggct tgagatagac
     1801 ctcgtgagga ccgaggatct caagccgggt gacctccggg gccgcgttgg cctggagaaa
     1861 attcacgttg agcttggaca ggaccggccc gctttcggcc gaggtcgatt tgaagagagc
     1921 ccggacctgg agataacgtc ctttcgggct gagaatcggc tcgccgtccg cattccggca
     1981 gggcggcgac cagtcgctcc aggtcggtcc cggctcggcc gtgttgccgc tccgcgattg
     2041 gagctggagc gcgccgccct ccggcacgac ggcctcccat tcgatccggc cccactggga
     2101 cgtcagattc gcgtcccaga caggcccctg gtattccccg ctcaaccgcc ggccggggga
     2161 aatccgggac aggccggccg gattgttgga caagagataa aagcgcggac cgtcgggaac
     2221 gcaggcgtag gcctgctcgg cctcgctttg cagaagcagg gccgccttct cccggcggtc
     2281 gagcgtgtag aggcgccctt tcggaccggt gccgaaataa agcttcccct cggcctcgtt
     2341 ccaaaacagg gtgtagacct gctcctcggg cgaaaaccag atccgccggg ccaggccgtc
     2401 ccgcccgatc cggtagacgg ccccgggctc cttgaggccg gcgacgacgg ccggccgggc
     2461 ggcggccgga ccggccggga cggccgcctg ggccgcggcc gagacggcga tcgagacgtc
     2521 gacgccgccg ggaacggcgg ccggagcgga aacggcctct ccgcgcggcg ccttgatcgt
     2581 gccgccggcc gagacataga ggttgccttc cggatcgaag gccgccgccc ggacttcctc
     2641 aaacggcgtc tcgaagacga cgacggtctt tccgccgttt ttctccagcc ggtagacaag
     2701 gcccgcgccg ccgcttccgg ccgtgagggc gccgtcggag ccgagcttta aacaaaggat
     2761 gtggttctga tcggatttga aaaccggccg gcccgagccc tcggggctga tctcgtagat
     2821 tccgccgctt tcgccgacgg ccgccaagag gcttcccgcc ggagacagga ccagatccca
     2881 gatgtaccgc tcctcgggat tgaagagctc ctgccccttg ccgggcgcgg tgattttgta
     2941 gatccggccg ttcggcgacg tcccggcgta aaggacgccc ttcggtccgg ttaaaagaca
     3001 ggtgacgtcc atctcggacg tctggaaata aagctcggcc ttgccgtcct tcccgagacg
     3061 gtagatcttg ccgccgtggc cggtgccgag atagccggtc ccgtcggaat ccagggccca
     3121 cgacaagtag aaatcttccg acgggccgtc gattttgtcc tcggggggag ccagggccag
     3181 gccgccgtcg gcctcgatgg cgatcccctc gctttttccg cggagaaagt cgtcgaacgt
     3241 ccggagcgtc cattgccggg gggcgacggc cgcggccgcc gcggccagga ggaggaccgc
     3301 cccaagaagg agtcgggcac gcttcatgat cattctccta attgcggatc ttgaggggaa
     3361 tcacggccag cccctcgaag acgaagggaa cgggcatctg gtattcccgc agcgtcgaaa
     3421 cgctgatttc aacaggcgcc gtggccgccg cccggggcga ggcgaacagc gatttcatcg
     3481 ccggcgtcag gttgggcatt tcctccccgc ggaggaacaa tcccggccgg gcggcgacga
     3541 tcttgaaata aacccgattg ttcttgcgga ggttgttgag aaggcggacc aattgctcga
     3601 ggctccgggg gacgatcccg ctcgtccggt actgtccgag ctcgacctgc cgcagggacc
     3661 gggtgtcggc cacgatcagg ttgaactcgg atccgggcgg aaggttcggg gccaggaaag
     3721 gaatctcctc cacccggctc tcgccccggt aggacttgat gaacagcttg agccggaggg
     3781 tttcgccggg cgcggcctcg taccggtcga gccaaacccg ctccaggaca gccgatttcg
     3841 gccgctccga ggtccggatg ttcaagtcga tccggtggat gccgacgtcc ttgaactcgt
     3901 tgttggtcag aaagtaaacc acggccgtga ccaggcccga aagatccgtc gtcgcccggc
     3961 cgaggtttcc ggtgaaaaga tcctcgatgt ggacggcctg gcccggcgac gtgtcgagat
     4021 agacgtcgcc gctgagctcg agggacaggt ccccgacggc ccggtcctcc gccccgatca
     4081 ggctggcgac ggccatgttc gccagaagcg gagtgaggag cttgtcggac gccagcttga
     4141 ggcggtactc gcgggccggc cccgattccc gggtcaaggt gatgttgagc gggacggttt
     4201 tcggaacgcg gccgatttcg ccgtaggctc cggacgtccg gtcctggacg acggccccga
     4261 tcaggctgcc gaccgagccc agcttgaacg aggagttgta cgcggggacg accgtgatga
     4321 tgtccgcctt ggccatgccg tattcgaccg ggccgaggtt gtacagggga tggccgaagg
     4381 cgtacacccg ggccccgtcg atgtaggtcg ccgttccgat ggccgagacg tcgaggtcgc
     4441 cggagatgag ctggacggcc agcggatctc cttccttgac gttgaggtcg gtgttcaagg
     4501 cgatgccggt ccgctggccg gccccggggg ccgaggccgc ccggacggag aatcccaggc
     4561 cggcgaaata cggccggatc tcgtcgagaa ggcggggcga aaagcccgaa aacaacaggg
     4621 gaacgggcaa ggccgcgctc gtccggccgt ccgcctcaaa cggcgccggc gaagagaaat
     4681 aatcccggtg cgcctcgaac aggtcccgca ggctcacccg ctcggaaaaa ggaagacggg
     4741 ccgttcccgg cccgggtttc ccggaggaat ctccggcgat ggccagcatc tcgccgatgg
     4801 gcgtgatgcc ggcgatcggc tccttggcga aggaaaagct gaaggccacg gccccgatga
     4861 tcttgccgtc gatgtacacg gggctgccgc tcatcccctg gagaacgccc gtctgctcga
     4921 ggttccggcc ggagagccgg gcgatgatgg cgtttcgctt ggggccggtg ttggccagga
     4981 cgccgaggat ctcgacgtcg aattcctcga ccttgttccc ggcgaagacg gtccggccct
     5041 tgcccttcat ccccgtcttg acgtcctcga ggggaaggat ctcggtcgcc gaacgggcca
     5101 agcccgcgcc caggatgacg gccagggccg tcgccgccaa aaccgcggtt tttctcatgt
     5161 tttaagcacc ttcaacggcc ggaaatccgt tttttgttcc atcccgtttg accgccgccg
     5221 ccggccgcgc taaaatcacc atttcatgcg cagcatcttg atcaccgggg cgacgggctt
     5281 catcgggggc catctcgccg agcgcctgcg ctcccggggg gaagccgtga ccgccctcgt
     5341 ccgcgattcc gcccgcggcc gctttctcga gagcctcggc gtccgtcttc ttcaggggga
     5401 attggacgcc gggccgcccc tgccggaaaa tctcgacgtc gtctttcatc tcgccgggct
     5461 gacccgggcg aggcgaaagg aagagtatta tatcgtaaac gaaaaaggca cggcaagcct
     5521 gctggacggc ctcccccggg gggaacgccg gccgcgattc gtctatctct cgacgacggc
     5581 ggccggaggg ccttcgctcc cggaccggcc ccgccgcgag gacgatccgg cccggcccgt
     5641 ttccgaatac ggccggtcca agcttctggc cgaagaagaa gtcctcgccc ggagggaccg
     5701 cctgcccgtc tccgtcgtcc gcgtcggccc ggtctacggg ccgcgggatc cgggctttct
     5761 cgattacttc aaatacgtcc ggcgcggctt tcttctttcc ttcggccggg aaccgcggcc
     5821 gatgtccgtc tgctacatcg acgacctcgt cgacggtctg ctggccgcgg ccgaaccggc
     5881 cgccggcacc ggcgaggtgt tcaacatcgg cgatcctacg ccccgcacgt tcgaggatct
     5941 cggcctggcc gccgccggct tgctgggcgt ccgcgcccgc cggatcatcc ttcccttccc
     6001 ggtcatccgg acggccgtgt ttttcgccga ggccgtccgc cccttcaccc ggacggtggg
     6061 catcgccaac cgggacaagt acaaggaata ccgggagaag gcctgggtgg cggatatgac
     6121 caaggcgaaa gcccgcctcg gtttcgaggc gtcctggagc ttggagaagg gtctggaaaa
     6181 gacgatcgcc tggtaccggg ataccggccg tctctgagaa agccgaagcc ccgtcagtcg
     6241 cccgaggcgg cggccttggc cgggatcgcc caagtcgaat aggccgtcga aaacttcgcc
     6301 gaggatttca aacaccagcc cccgacccgg tcgaaggcga aatacttgac ccggccgcgc
     6361 aggacataaa ccccccgccg ggctttctgg ccgacggtga acggaatttc gatcggccct
     6421 tggagatcaa ggcagtcgcg gccgtcgatc tcgacgtggg ccaggtcgag atccgacccg
     6481 gtgaaaaaat tcttgggaaa gacgagatcc ggtccttcct cgacttcgat cttgaacgcc
     6541 gggagagccg agacggcgat ccccttcctc aagctgatct tgaggacgac tttcccttcc
     6601 tggccccggg ccaatcgccg gggggcgatc ccggcttcca ctttaataag gtcctcggcg
     6661 gccgcccgca aaattccccc ggtcagaacc agggcggcgg cggcgatcgc gagatacccg
     6721 gattttctca taagggaagc acccgccgga cagtttaaaa gccccccgcg
//
LOCUS       DAOOXH010000112         6590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_13317_length_6590_cov_7.221576, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000112 DAOOXH010000000
VERSION     DAOOXH010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13317_length_6590_cov_7.221576"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1958)
                     /locus_tag="PKV64_10310"
     CDS             complement(105..1958)
                     /locus_tag="PKV64_10310"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPN17505.1"
                     /translation="MDMLLKCPSCQADNQPDSRFCSRCATPLPQEAPSDSDGLTRTMI
                     ASIDRLERGILFAGRYEIIEEIGRGGMGRVYRAYDRQLKEDVALKLLKPEISFNEKAV
                     ERFRNELKFARRIAHPRVCRMYDLGESGLAHYLTMEYVEGEDLKAFIRRSDHLTTAKA
                     LDIGRQIAEGLEEAHRLGVVHRDLKPQNVMIDREGKAKIMDFGLARISETEGLTGSGL
                     MLGTPEYMSPEQVDLKDVDGRVDIYALGIILYEMVTGHVPFKGETPLAVALKQKNEKA
                     RDVRESNPLVPDTLISVILKCLEKDPGRRYQTAAETSEALLEVERGLSSAVLEISKTA
                     TRGSSAHLRPRRIKRAVAVSAALAVLAAAIAAVVFLTGKRNPAPSARHSSIVPTLEGA
                     EEESVRTGRALLGILGSKDPENVKAVEKTLKEMRRYLPESGPSVEAWKQAQEKFERSR
                     AGDPASAGAPEIGGEMQQLMAVISERESARRSRDSMILVKTGLRGWSADNNFLFRLAQ
                     FEERNADDAFAKNDYAGSRALYRLLERIYLAVPNGKTAARGVVALREIVKSLKTEAAV
                     VPEAKIDSWLASSAREIAAEAESFASKGDLENACGAFTRAAFLLQKIRDTG"
     gene            2118..3023
                     /locus_tag="PKV64_10315"
     CDS             2118..3023
                     /locus_tag="PKV64_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPN17506.1"
                     /translation="MKPALVILAAGIGSRYGSLKQVDPVGPSGETLMDYSLFDALRSG
                     FGRCVFIIQKSMEQDFRDVILKKLEGRMEADCAFQAIDDLPSGFTVPPERTKPWGTSH
                     AVLAAASKIREPFGVINADDFYGRDAFQKMAAFLSDVGAAESRYAVVGYDLDATLSDY
                     GAVARGVCEVNPAGFLTGIVERTRIIRSKFGIVFEDPSGRHISLGEGRTVSMNFWGFT
                     PTYFQFARTEFVRFLEERRNDPKAEFFIPLVVNRMIEDGLADCLVLPTTARWFGVTYK
                     ADRPAVMASLESLIAAGEYPRSLWG"
     gene            complement(3027..4736)
                     /locus_tag="PKV64_10320"
     CDS             complement(3027..4736)
                     /locus_tag="PKV64_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPN17507.1"
                     /translation="MRGKTIRRRAWTPVLFVGAGLAAFAGDGNFIPTPAEAAGYREYT
                     RNESVAAFLSELAARAPALRVSAVGRSLPTEACGAMDLFLAVLAEKPAAAPAELDRTK
                     PTVLITAAQHGNEQSAKEAALRLVRDLAAGELSPLLERLNVLVMPQTNPCGNLRNVRG
                     NELGLDMNRDHVKLETEGVRAIHRVFRDWMPEVTIDVHEKGDDYYRISIGCVSNANID
                     AGLQDFSRNTLLAETGRALEKRKVTFHEYLVTEELGVDTSSGSARAAERPAAREEMKR
                     YSTTDLNDGRNSLGIFETLSFIQEGASRHDLETLQPRTDWQYDGLRAFLESAAGHAPE
                     ILELVRSRRARLLERAAGRLPGDDVRLRMKYVRDPAVPELRLKRFDREGPPVRGVLRV
                     DKKAGEAVTFADLLPPLEPGGAAVVDEIVKNWFPLVESTLGVSRPAGYLFEGGRPEIA
                     ETLRALGVEVGMFARDVLVEAEVYEVESIIPARFDYDPPERIDVAAKAARLPVRKGDF
                     YVSCVQPAAHLVPCLLEPQSDYGFIRYGKFKLIPEAGGLYEIRRYAGKEDLPVVPYRP
                     WRP"
     gene            complement(4878..5456)
                     /locus_tag="PKV64_10325"
     CDS             complement(4878..5456)
                     /locus_tag="PKV64_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPN17508.1"
                     /translation="MPKSIKGTKTEKNLLTAFAGESQARNRYTYFASAAKKEGYEQIA
                     AIFLETAENEKEHAKVFFKALEGGAVEIMAAYPAGIIGDTAANLKAAADGELEEWSVI
                     YKDFAGVADAEGFPEVATSFRMIAKVEREHENRYRKLLANVTGGKVFKKDAPVRWMCR
                     NCGYVHEGVEAPAKCPACLHPLAFYEIKAENY"
     gene            complement(5475..5858)
                     /locus_tag="PKV64_10330"
     CDS             complement(5475..5858)
                     /locus_tag="PKV64_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HPN17509.1"
                     /translation="MAKAKERFQIFHCPVCGNIVEVLTAGGGELVCCGRPMELMTENT
                     VDASKEKHVPVIEKTADGYKVKVGSVPHPMEEKHYIMWIELIADGRACRKFLKPGEAP
                     EAFFRLEAEKITAREYCNLHGLWKA"
     gene            complement(5851..6420)
                     /locus_tag="PKV64_10335"
     CDS             complement(5851..6420)
                     /locus_tag="PKV64_10335"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPN17510.1"
                     /translation="MLSTKKIFVNAPAGALTTGRKSTIIQFVMVSNTSRLRDILAERG
                     IKPTFQRLTILGAVLDNRSHPTIRDLHAHLVRTIPTLSKTTLYSTLERFAAEGLVRPL
                     MIDPAEVRYDGAPALHHHFQCTACGRILDIEISCPNSRAGEIHGHRIDEVHGYFKGVC
                     RDCLSTPGRSAGAPASHKTQLKKRRKSHG"
     gene            6451..6576
                     /locus_tag="PKV64_10340"
     CDS             6451..6576
                     /locus_tag="PKV64_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17511.1"
                     /translation="MKRTRVLPIMAAAGLILVFSASAAAPETWIVDILANNGGDI"
BASE COUNT         1096 a         2044 c         2146 g         1304 t
ORIGIN      
        1 gatgttcgct ccggccgtca taacttccgc gcttcggccc cgctccgggg acgtgcgcgc
       61 caggactttt tggcgctcgg cttggatcaa gcgttgatgg cgcttcagcc cgtgtcgcgg
      121 attttttgaa gcaaaaaggc ggcccgggtg aacgctccgc aggcgttctc gaggtcgccc
      181 ttggaggcga atgattcggc ctcggccgcg atctcccggg cggaggaggc cagccaggag
      241 tcgatttttg cctcgggaac gaccgcggcc tcggttttca ggctcttaac gatttcccgg
      301 agggcgacga cgccgcgggc cgcggtcttg ccgttgggaa cggccaggta gatccgttcc
      361 agaagccggt agagggcccg ggacccggcg taatcattct tggcgaaagc gtcgtcggcg
      421 ttgcgttcct cgaactgggc cagccggaaa aggaagttgt tgtcggccga ccagcctcgg
      481 aggccggttt tcaccaggat catggaatcc cgggaccggc gggcggattc ccgttcggag
      541 atgacggcca tcaactgctg catttcgccg ccgatttcag gagcgccggc cgaggccgga
      601 tcccccgccc ggctccgctc aaatttctcc tgggcttgtt tccaggcttc cacgctcgga
      661 ccgctttccg gcaggtatcg gcgcatttcc ttgagcgtct tttccacggc cttcacgttt
      721 tcgggatcct tggaaccgag aatgccgaga agcgcccggc cggtacggac ggactcctcc
      781 tcggcgcctt cgagggtcgg gacgatcgag gagtgccggg ccgatggggc cggattccgt
      841 tttccggtca ggaatacgac agcggcgatc gccgcggcta aaacggccaa ggccgccgaa
      901 acggcgaccg cccgcttgat ccgccggggg cggaggtgcg cgcttgagcc gcgggtcgcg
      961 gtcttggaga tctccaggac ggccgaggac aatcctcgct cgacctccag aagggcttcg
     1021 gacgtctccg ccgcggtctg atagcggcgg cccggatcct tttccaggca tttcaggatc
     1081 accgagatca gcgtatccgg cacgagcgga ttcgattccc ggacgtcacg ggccttttcg
     1141 tttttctgtt tcaaggcgac ggccaaaggt gtttcgccct taaacgggac atgcccggtg
     1201 accatctcgt aaaggatgat gcccagggcg taaatgtcga cccggccgtc gacgtccttg
     1261 aggtcgacct gttcggggga catgtattcc ggagttccga gcatgagtcc ggatcccgtc
     1321 aacccctcgg tttcggagat tcgggccagc ccgaaatcca tgattttcgc cttgccctcg
     1381 cggtcgatca tcacgttttg gggcttgagg tctcgatgga cgacccccaa gcggtgggct
     1441 tcctccaggc cctcggcgat ctgccggccg atgtccaggg ccttggccgt cgtcaggtga
     1501 tcggagcggc ggatgaaggc tttcaggtcc tcgccttcga catattccat ggtcaaataa
     1561 tgggccagcc cggattcacc caagtcgtac atccggcaga cgcggggatg ggcgatccgg
     1621 cgggcgaact tgagctcgtt gcggaagcgt tcgacggctt tttcgttgaa gctgatttcc
     1681 ggcttcaaaa gcttgagggc gacgtcttcc ttcagctggc ggtcataggc ccggtaaacc
     1741 cggcccatgc cgccccggcc gatctcctcg atgatttcgt accggccggc gaagaggatt
     1801 ccgcgctcca ggcggtcgat cgaggcgatc atcgtcctgg tgaggccgtc cgaatcggag
     1861 ggcgcttcct ggggcagggg agtcgcgcag cgggagcaga accggctgtc gggctggtta
     1921 tcggcctggc aggaagggca tttgagaagc atatccatca cttaccatgc gacttacagg
     1981 ttgtcaacgt ccttgttccc ggcctctgct gatatatacg taaaacaccc ccgttacgtt
     2041 gccgaattcc ggccgcggcc ggaggtggaa ctcggatcgc ctttatgata ataaggatca
     2101 ttcaggagga cccgatgatg aagcccgccc tggtgatcct ggccgccgga atcggaagcc
     2161 gttacggcag cctgaaacag gtcgatcccg tcggcccctc aggcgagacg ctgatggatt
     2221 attcgctgtt cgacgccctc cggtcgggat tcggccggtg cgttttcatc atccagaaga
     2281 gcatggaaca ggatttccgg gacgtcatcc tcaagaagct cgagggccgg atggaagcgg
     2341 attgcgcctt ccaggcgatc gacgacctgc cgtcgggatt taccgttccc cctgagcgga
     2401 ccaagccttg ggggacgtcc catgccgttc tggcggcagc gtccaagatc cgcgagccgt
     2461 tcggcgtcat caacgccgac gatttttacg gccgggatgc ctttcagaaa atggccgcct
     2521 ttctctccga cgtcggggcc gccgaatccc gctacgccgt cgtcgggtac gacctcgacg
     2581 cgaccctgtc ggattacggg gccgtcgccc ggggcgtctg cgaggtgaat ccggccgggt
     2641 tcctgaccgg catcgtcgag cggacccgga tcatccggag caagttcggg atcgttttcg
     2701 aggatccctc cggccgccac atctccctgg gcgagggacg gaccgtgtcg atgaatttct
     2761 ggggattcac cccgacctat ttccagttcg cccggacgga gttcgtccgg ttcctggagg
     2821 agcggcggaa cgatccgaaa gccgagttct tcatccccct cgtcgtcaac cggatgatcg
     2881 aggacggcct ggccgattgc ctggttctcc cgacgaccgc ccgttggttc ggcgtgacct
     2941 acaaggcgga ccggccggcc gtgatggcct ccctcgagag tctgatcgcc gccggggaat
     3001 acccgcggag cctctggggc tagcggctaa ggccgccaag gccggtaggg aacaaccgga
     3061 aggtcttctt tcccggcgta ccggcggatc tcgtaaagcc ctccggcctc ggggatcagc
     3121 ttgaatttcc cgtaccggat gaagccgtag tcggactggg gctccagaag gcagggaacg
     3181 agatgagccg ccggctggac gcaggagaca tagaaatcgc ccttccggac cgggaggcgg
     3241 gcggctttgg ccgcgacatc gatccgttcc ggggggtcgt aatcgaaccg ggccgggatg
     3301 atcgactcga cctcatagac ttcggcctcg acgagaacgt cccgggcgaa catcccgacc
     3361 tcgactccga gggcccgcag cgtctcggcg atctccgggc ggccgccctc gaaaagataa
     3421 ccggccgggc gggaaacgcc gagagtcgac tcgacaagag ggaaccagtt cttgacgatc
     3481 tcgtcgacga cggcggcgcc gcctggttcc agcggcggga ggagatcggc gaaagtcacc
     3541 gcctcgcccg cttttttatc gacccggaga acgccgcgga cgggcggccc ttcccggtcg
     3601 aaccgcttga ggcggagctc gggaacggcg ggatcgcgga cgtacttcat ccggagacgg
     3661 acgtcgtctc cgggcagccg cccggcggcc cgttcgagca gacgcgccct ccggctccgg
     3721 accagttcca ggatttccgg ggcatggccg gccgccgatt cgaggaaggc ccgcaggccg
     3781 tcgtattgcc agtccgtccg cggctggagg gtttccaggt catggcggga cgcgccctcc
     3841 tggatgaacg agagggtttc gaaaatcccc aggctgttgc ggccgtcgtt gaggtcggtc
     3901 gtcgagtagc gcttcatctc ctcgcgggcc gccgggcgtt ccgccgcccg ggccgagccg
     3961 gacgaggtgt cgaccccgag ttcctccgtg acaaggtatt catggaacgt gaccttccgt
     4021 ttttccaggg cccggccggt ctcagccagg agggtattgc gggagaagtc ctggagaccc
     4081 gcatcgatgt tcgcgttcga gacgcagccg atcgagatcc ggtaatagtc atctcccttt
     4141 tcgtggacgt cgatcgtcac ctcgggcatc cagtcccgga agacgcggtg gatggcccga
     4201 accccctccg tttccagctt gacatgatcg cggttcatgt ccaggccgag ctcgtttccc
     4261 cggacgttcc ggagattgcc gcagggattg gtttggggca tgaccaggac gttgaggcgc
     4321 tcgaggaggg gggacagctc gcccgcggcc aggtcccgca ccagccggag agccgcttcc
     4381 ttggccgatt gctcgttgcc gtgctgggcg gcggtgatga gaaccgtcgg tttcgtccgg
     4441 tcgagctccg ccggcgcggc cgcgggcttt tcggccagga cggccaggaa gagatccatc
     4501 gccccgcagg cttccgtggg caaggatctc ccgacggccg agacgcggag ggcgggcgcc
     4561 cgggcggcca gctcgcttaa aaaagcggcg acggattcgt tccgcgtgta ctcccggtat
     4621 cccgcggctt cggccggagt cgggatgaaa ttcccgtcgc cggcgaaggc ggccagcccg
     4681 gccccgacga acaagaccgg cgtccaggct cgtctccgga tggtctttcc cctcatgact
     4741 tttcctcccc gttgatttcc gcttccgttg aatcacagag ccggcttcat gcgggcgggc
     4801 ttccgaatcc gcccggccgc ggaagccgtc ggctcaaacg cgcccgcctg gaaaaaacgc
     4861 ggcccgggac gcgccggtca ataattctcg gctttgattt cgtagaaagc caggggatgg
     4921 agacaagccg ggcacttggc cggggcttcg acgccttcgt ggacgtagcc gcagttgcgg
     4981 cacatccagc ggaccggagc gtccttcttg aagactttcc ctccggtcac gttggccagg
     5041 agcttgcggt agcggttttc gtgttcccgt tcgaccttgg cgatcatccg gaaagaagtc
     5101 gcgacctcgg ggaatccctc ggcgtcggcg acgccggcga aatccttgta aatcaccgac
     5161 cactcctcga gctcgccgtc agccgcggcc ttgaggttgg cggccgtgtc cccgatgatc
     5221 ccggccgggt aggcggccat gatctccacc gctccgccct ccagggcttt gaagaagact
     5281 ttggcatgct ccttctcgtt ttccgcggtc tccaggaaga tcgcggcgat ctgctcataa
     5341 ccctcttttt tcgcggccga ggcgaaatac gtgtagcggt tgcgggcctg ggactcgccg
     5401 gcgaaggcgg tcaacaggtt tttctcggtc tttgtccctt tgatggattt cggcatgacg
     5461 ttctccttgt ttgttcaagc cttccacagg ccgtgaaggt tgcagtattc gcgggccgtg
     5521 attttttccg cttcgaggcg gaagaaggct tcaggcgcct ccccgggctt caggaatttc
     5581 cggcaggccc ggccgtcggc gatgagctcg atccacatga tgtaatgctt ttcctccatg
     5641 ggatggggga cggatccgac cttgaccttg tacccgtcgg cggttttctc gatcaccggg
     5701 acatgctttt ctttggacgc gtcgaccgtg ttttccgtca tcagctccat cggccggccg
     5761 cagcagacca actcgccgcc gccggcggtc aacacttcga cgatgttgcc gcagacgggg
     5821 cagtgaaaga tttgaaaacg ttccttggct ttagccatga ctcttcctcc tttttttcag
     5881 ttgggtttta tgggatgccg gcgcgccggc ggaccggccg ggggtgctca ggcaatcacg
     5941 gcagacgccc ttgaaatagc cgtggacctc gtcgatccgg tggccgtgga tctcgccggc
     6001 ccggctgttg ggacaggaaa tctcgatgtc caggatgcgg ccgcacgccg tgcactgaaa
     6061 atgatggtgg agggccggag cgccgtcgta gcggacttcg gccgggtcga tcatcagcgg
     6121 gcggaccagc ccctcggcgg cgaaccgctc cagggtggaa tacagggtgg tcttggacag
     6181 ggtcggaatc gtccggacga gatgggcgtg aagatcccgg atcgtcggat gagaccggtt
     6241 atcgaggacg gccccgagga tcgtcaaccg ctgaaacgtc ggcttgatcc ctcgttccgc
     6301 caagatgtcg cgcaaacggg acgtatttga aaccattaca aactggatta tcgtcgattt
     6361 ccggccggtt gtcaaggcgc cggccggcgc gttgacaaag attttttttg tggatagcat
     6421 gatgtcaaat ccgcggagag gaggagtccc atgaagagaa cccgggtcct tccgatcatg
     6481 gccgccgccg gcctcattct cgtcttcagc gcttcggccg ccgccccgga gacctggatc
     6541 gtcgacattc tcgccaataa cggcggggat atctgaacaa ggtcggcgtg
//
LOCUS       DAOOXH010000113         6561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_13414_length_6561_cov_8.490470, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000113 DAOOXH010000000
VERSION     DAOOXH010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13414_length_6561_cov_8.490470"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PKV64_10345"
     CDS             complement(<1..296)
                     /locus_tag="PKV64_10345"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPN17512.1"
                     /translation="MSAFFAYAFLQRALAAGLCLAAACAVLGVFLLLRKDAMIGHGLS
                     HVAFAGIALGLLVGRWPIAVALAVSVGAALGIMKLREAAGIHGDTAIAVDAGRLS"
     gene            complement(313..1074)
                     /locus_tag="PKV64_10350"
     CDS             complement(313..1074)
                     /locus_tag="PKV64_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN17513.1"
                     /translation="MASPDADKGELIRVEDVSFNYGGLPVLKDISFTISAGDFLAILG
                     PNGSGKTTLLRVILGLARPEKGRVFILGKPAETFAGRELIGYVPQKATHFDPLFPASV
                     REVVAMTLRSGRGRESRKAEEAEIRRALDIVGMTGVMDRRIGRLSGGQQQRVFIARAI
                     VRKPRILFLDEPTAGVDAETQDRFYDLLGSLHAEEKITIVLVTHDIGIINKHVTKVAC
                     LNQRLVYHGTHDHFCSSGALRELLDGGRHLVSHRH"
     gene            complement(1055..1987)
                     /locus_tag="PKV64_10355"
     CDS             complement(1055..1987)
                     /locus_tag="PKV64_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPN17514.1"
                     /translation="MKTAGLFALAAFSVVAMAAAFPADSSEGGDGRLRAVATVLPLAE
                     FARDIAGGRAEVVLLLPPGTDVHTWQPRVSDIRTLEKADLLISVGLGLEPWLESLVRG
                     AAAGRLRRFEASAGLDLIAAAGEDHEDDHRHGAFDPHVWLDFTQDEAIVDGLVRAFSS
                     LAPADAGFFARNAEALKARLRALDASFREALAPFRGREFILAGHAAFAYLARRYELRQ
                     VAVYGPSPDAAPTPRETAEIISRAASAGVKTIFYEWAAGDKMARLIAAEIGADVRVLY
                     PGHNLDPAQSVRGLTFFRLMEENRENLAHGLSGR"
     gene            2097..3554
                     /locus_tag="PKV64_10360"
     CDS             2097..3554
                     /locus_tag="PKV64_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HPN17515.1"
                     /translation="MSDDLSGLKPQPLWKYFEEICRIPHGSGHEKALADFVLAWAKSR
                     KFAAKKDAAGNVFLAKPASPGKDSSPGIILQGHLDMVWEKNSDVAHDFLKDPIKPVIR
                     DGWVGAAGTTLGADNGIGVAAAMAIFEDDALAHGPLEALFTVEEETGLTGARAITSTA
                     LKGKYLLNLDSEEEGAFTIGCAGGADSDFTLPLARKAVKTKTAFRLMISGLRGGHSGI
                     NIHEGRANAVKMLARVLGQAQAKHSFALYRMEGGNKHNAIPREAWADFYVEPAKIKGF
                     SSFLQAAGDQIKAEYRVVEPGMKWTLAPVEPGVKDIPLTPKSQKTLLDFLVCCPHGVV
                     SMHAEISGLVETSTNLAVLRTAKDKARILCSSRSSVGSALAALRAGLKSLADLCGAKV
                     RQPSGYPGWTPNLQSTLLQKMKTVHKAVTGKDAAVLAIHAGLECGLIGEKYPEMDMIS
                     LGPTLQYPHSPDERVHIGSVENFWKLLRASIKALA"
     gene            3684..>6561
                     /locus_tag="PKV64_10365"
     CDS             3684..>6561
                     /locus_tag="PKV64_10365"
                     /inference="COORDINATES: protein motif:HMM:NF037694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPN17516.1"
                     /translation="MPRKRAAIPLILAALVFLIPAAGCRRKAPSRLGEAVKGEIFGTL
                     GVVHAGPRGATAGVRESDEIVVVFDHPMAPLAERPFEDATAVFKIEPGVKGAFRWMGT
                     RTLAFAPESRLPYATEFKITIPSGTRSLDGFALDKDYFWTFETPRPRLVRHLPAGDEE
                     QLRLETEAVLVFNQPVEAAKVRPWVIFTGADASGRTEQPAFDLLRPDEKVLKEAELPF
                     PPERVVLLHPRAKLQPGFSYAVEIRAGLTGREGRLPLEENAVFRFSTFETFRFDGLEE
                     EVNRDPRRALSFRFSNRVRYKEFAEKIRIEPKVEVPEYYREWDHGHTTLWVSLPLQPE
                     TAYTLTLPADLRDDFGNPLGREETCEFTTGPLSPFVGLATGSGVIESYGALTFPLFAV
                     NSPEIRVRAARVQPDQAIPLLTSEKAFSGRSSFQPHAGFYSYDRILRPNLPRNERGFV
                     PVVLSEIESDGRGFVFLEVETGSEDEWSRYAKTFLQLTGLGISGKFSPENNVVWVSFL
                     KTGQPAPGAEIEIRDDQNRVLWKGTAGPDGRAETPGWKTLGLRPRDEWTKPRQWVYAR
                     LGDDVALAASDESEGVEPYRFDLMYDWNPEPVRFQGTLFSERGIYRAGETVHLKGIFR
                     ERSKGAWVIPAVREAQLEIQDPFSKSVSKAKVDLDAYGSFAFDFDTREDAPLGAYSVT
                     AVIPKSSSNAVEARAYDSFRLDAFRPAEFEVHLKSLRDAYVIGDAYEAEIRASYLFGG
                     VLAGQPASWALRLNPAGFTPPGRPGFTFGREAEEWSEDMPSETSRLVGSGEGSLDKDG
                     RLAVKVPLAAGKETGTLSADLEVTVQSPSRRSISSRIQALVHPGDFYLGLKPSTSFLK
                     KGDPLSVQVIAALPDGTLQGGRAVKVKLLRRIWKSARKAGLGGRLEWISEKSDTVVAV
                     RDVKTADSEPAAAAFAPDQSGFYLVVAEGRDGSGRAVSSSTYLYV"
BASE COUNT         1214 a         2125 c         2061 g         1161 t
ORIGIN      
        1 gagaggcggc cggcatccac ggcgatggcc gtgtcgccgt ggatgccggc cgcctctcgg
       61 agcttcatga tgcccagcgc ggccccgacc gagacggcga gggcgacggc gatcggccaa
      121 cgaccgacga ggaggccgag ggcgatcccg gcgaaggcga catgggaaag cccgtgtccg
      181 atcatggcgt ccttgcggag aagaaggaaa acgcccagga cggcgcaagc cgccgccagg
      241 cacaggccgg ccgccaaggc tctctgtaaa aacgcatagg cgaaaaacgc gctcatgacc
      301 gccgcctcac gttcaatgcc ggtgggaaac gaggtgacgt cccccgtcca ggagctccct
      361 caaggccccc gaggagcaga aatggtcgtg ggttccatgg tagaccaggc gctgattgag
      421 acaggcgacc ttggtcacat gcttgttgat gatgccgata tcatgggtga caaggacgat
      481 cgtgattttt tcctcggcgt gaagcgatcc caagaggtca tagaaccgat cctgcgtttc
      541 cgcgtcgacg ccggccgtcg gctcgtccag gaacaggatc cgcggctttc ggacgatggc
      601 ccgggcgatg aacacgcgtt gctgctgacc gcccgacaac cgtccgatcc ggcggtccat
      661 gacgccggtc atgccgacga tgtccaaagc ccggcggatc tccgcttcct cggccttccg
      721 ggattcacgg cctcgcccgg agcggagcgt catggccacg acttcccgga ccgaggccgg
      781 gaagagggga tcgaagtggg tcgctttttg ggggacgtag ccgatcagct cccggccggc
      841 gaaggtttcg gccggcttgc ccaggataaa aacccggcct ttctcaggcc gggccaagcc
      901 caggatgacc cgaagaagcg tggtctttcc cgacccgttg ggcccgagga tcgctaaaaa
      961 atcaccggcc gaaatcgtga acgagatgtc tttcagaaca ggaagaccgc cgtagttgaa
     1021 ggagacatcc tcgacccgga tcagctctcc cttgtcagcg tccggagagg ccatgagcca
     1081 aattctcccg attctcttcc atcagccgga agaaggtcag gcctcggacc gactgggcgg
     1141 gatcgaggtt gtgcccggga tagagaaccc ggacgtcggc cccgatttcg gcggcgatca
     1201 agcgggccat cttgtcgccc gcggcccatt cgtaaaaaat cgtcttgact cccgcggacg
     1261 cggctcggga gatgatctcc gctgtttccc gcggcgtggg agccgcgtcc gggctcggcc
     1321 cgtagacggc cacctgccgg agctcgtacc gccgggccag gtaggcgaag gccgcgtggc
     1381 cggcgagaat gaattcccgg ccgcggaacg gggcgagggc ctcgcggaac gacgcatcga
     1441 gcgcccggag ccgcgctttc aaggcctcgg cgtttcgggc gaagaatccg gcatcggccg
     1501 gggcgaggct ggaaaacgcc cggaccaagc cgtcgacgat cgcctcgtcc tgggtaaaat
     1561 ccagccaaac gtgaggatcg aaggcgccgt gacggtgatc gtcttcgtga tcttcgccgg
     1621 cggccgcgat caggtccaag ccggccgagg cttcaaaccg gcggaggcgc ccggccgcgg
     1681 ctccgcggac cagcgattcc agccagggtt cgagacctaa gcccacggaa attaaaagat
     1741 cggctttttc cagggtccgg atgtcgctga cccggggctg ccaggtatgg acgtcggtcc
     1801 cgggaggcag gagcagaacg acctcggccc ggccgccggc gatgtcccgg gcgaattcag
     1861 ccaggggaag aacggtcgcc acggctctga ggcggccgtc tccgccctcc gaggaatccg
     1921 ccgggaacgc cgccgccatc gcgacgacgg agaatgcggc gagagcaaaa aggccggcgg
     1981 ttttcatggg ctttcaaatt ctaggggccc cccggggaaa tgtcaaaccg cccggcggag
     2041 tttgccaaga gacgaggctt gtggcatgat aagaaaccat aaaatggagg ttccccatgt
     2101 ctgatgattt atccgggctc aagccccaac ccctttggaa gtacttcgag gaaatctgcc
     2161 ggattcccca cggttccggc cacgaaaaag ccctggcgga tttcgtcctg gcctgggcca
     2221 agtcgcggaa gttcgcggcg aaaaaggacg cggccggaaa cgtcttcctc gccaaaccgg
     2281 cgtccccggg caaggattcc tcgccgggaa tcatcctcca aggccacctc gacatggttt
     2341 gggagaagaa ttccgacgtc gcccacgatt tcttaaagga tccgatcaag ccggtcatcc
     2401 gggacggctg ggtcggagcg gccggcacga ccctcggggc cgacaacggc atcggcgtgg
     2461 ccgcggccat ggcgattttc gaggacgacg ccctggccca cggcccgctc gaggctctct
     2521 tcaccgtcga ggaggaaacc ggattgaccg gagcccgggc gatcacgtcc acggccctga
     2581 agggcaaata cctcctcaac ctcgacagcg aggaggaagg cgcgttcacg atcggatgcg
     2641 ccggcggcgc cgattcggac ttcacccttc ccttggcccg caaagcggtc aagaccaaga
     2701 ccgccttccg cctgatgatc agcgggttgc gcggcggcca ttcgggcatc aatatccacg
     2761 aaggccgggc caacgccgtc aagatgctgg cccgcgtcct cggccaggcc caggccaaac
     2821 attccttcgc cctgtaccgg atggaaggcg gcaacaagca caatgccatc ccgcgcgaag
     2881 cgtgggccga tttttacgtc gagccggcca aaatcaaggg cttttcctct ttcctccagg
     2941 cggccgggga tcaaatcaag gccgagtacc gtgtcgtcga gccggggatg aaatggacgc
     3001 tcgcgccggt cgagccgggc gtcaaggata ttcccctgac tccgaaaagc cagaagacgc
     3061 tcctcgattt tctggtctgc tgcccccacg gcgtcgtttc gatgcacgct gaaatcagcg
     3121 ggctcgtcga aacatccacc aacctggccg ttttacgcac ggccaaggac aaggcccgca
     3181 ttctctgctc cagccggagc tcggtcggat ccgccctggc cgcccttcgg gccggcttga
     3241 agtccctggc cgatctttgc ggagccaagg tgagacagcc gtcgggatac cccggctgga
     3301 cccctaacct tcaatcgacc ctcctgcaaa aaatgaaaac cgtccacaag gccgtgaccg
     3361 ggaaggacgc cgccgtcctg gccattcatg ccggcctcga gtgcggcttg atcggggaga
     3421 aatatccgga gatggacatg atttccctcg gtccgacgct ccagtatccg cattcgccgg
     3481 atgagcgggt ccacatcgga tccgtagaga atttctggaa attgctccgg gcctcgatca
     3541 aagcgctcgc ctgagcgtcg aacgcctgat ccgccccgtc ccctcccggg ggggggacgg
     3601 ggcttttttt taccggacgt cttcttgaag tttagaaggc attccgttag catgaactca
     3661 attcccgtct tggaggattg ttcatgccaa gaaaacgcgc cgcgattccc ctcattctcg
     3721 ccgcactcgt tttcctgatc cccgcggccg gctgccgacg gaaagcgcct tcccggctgg
     3781 gggaagcggt gaaaggagag atcttcggaa ccctcggcgt cgttcatgcc ggaccgcgcg
     3841 gggcgacggc cggcgtccgg gaatcggacg aaatcgtcgt cgttttcgat caccccatgg
     3901 cccctctcgc cgagcgtccc ttcgaagacg cgaccgcggt tttcaagatc gagcccggcg
     3961 tcaagggcgc cttccggtgg atggggacgc ggaccttggc cttcgcgccc gaatcgcgcc
     4021 ttccgtacgc gacggagttt aaaatcacca ttccgtcggg gacccggtcc ctggacggct
     4081 tcgccttgga taaggattat ttctggacct ttgaaacccc ccggccgcgg ctggtccgcc
     4141 atctgccggc cggcgacgag gaacagctcc gcttagagac cgaagccgtc ctcgtcttca
     4201 accagccggt cgaggcggcg aaggtccggc cctgggtcat tttcaccggg gccgacgcct
     4261 cggggcggac ggaacaaccg gccttcgatt tgttgcggcc ggacgaaaaa gtccttaagg
     4321 aagcggagct tccttttccc cccgaacggg tcgttctcct ccacccccgg gcgaagctcc
     4381 agccgggatt ttcctatgcc gtggagatcc gggcagggct gaccggccgg gaaggccgtc
     4441 tccccttgga ggaaaacgcc gtcttccgct tttccacatt cgagaccttc cgtttcgatg
     4501 gattggagga ggaggtgaac cgggatcccc gccgggccct ttctttccgt ttttccaacc
     4561 gggtccgtta caaggaattc gctgaaaaga tccggatcga accgaaggtc gaagtcccgg
     4621 aatattaccg ggaatgggat cacggccaca cgacgctttg ggtgtctctc cccctccagc
     4681 ccgaaaccgc ttacaccctg acgctgccgg ctgatctgcg ggacgatttc ggcaatccgc
     4741 tgggccggga ggaaacctgc gagtttacga caggccccct gtctcccttc gtcggcctgg
     4801 ccacgggctc cggcgtgatc gaatcctacg gcgccctgac gttccctctt ttcgccgtca
     4861 acagccccga aatccgggtc cgggcggccc gggttcaacc ggaccaggcc attccgctgc
     4921 tcacctcgga gaaagcattt tccgggcggt cttccttcca gccgcacgcc ggcttctatt
     4981 cctacgaccg gattctccgt cccaacctgc ctcgcaacga gcggggcttc gttcccgtcg
     5041 tcctgtccga gatcgagtcg gacggccgcg ggttcgtatt cctcgaggtc gagacaggtt
     5101 ccgaggacga gtggtcgcgg tatgccaaga cgtttctcca gctgaccggg ctcggcatct
     5161 cggggaaatt ttcgccggaa aacaacgtcg tctgggtttc gttcctcaaa accggccaac
     5221 cggcgcccgg ggcggagatt gaaatccggg atgatcaaaa ccgggttctc tggaagggaa
     5281 cggccggccc ggacgggcgg gcggaaacgc cgggctggaa aaccctcggc ctgcgtccgc
     5341 gcgacgaatg gaccaagccc cgccagtggg tttacgcccg gcttggagac gacgtcgccc
     5401 tggcggcctc ggatgaatcc gaaggcgtcg agccgtaccg cttcgacctc atgtacgatt
     5461 ggaaccccga gcccgtccgt ttccagggaa cgcttttctc cgaacgcggg atctaccggg
     5521 ccggcgagac cgtccacctc aaggggatat tccgcgagcg ctcgaagggc gcctgggtga
     5581 ttcccgcggt tcgggaagcg caactggaaa ttcaggaccc tttttcgaaa tccgtctcca
     5641 aggccaaggt cgacctcgac gcctacggat ccttcgcctt cgatttcgac acccgcgagg
     5701 atgctcccct cggagcttac agcgtcacgg ccgtcattcc taaatcatca tcgaacgccg
     5761 tggaagcgcg ggcttatgac tccttccggc tcgatgcctt ccgtccggcc gagttcgaag
     5821 tccacttgaa atcgctccgg gacgcttacg tcatcggcga cgcctacgag gcggaaatcc
     5881 gggcgtccta tctcttcgga ggggtgttgg ccggccagcc cgcctcctgg gctctccggc
     5941 tgaatccggc cgggttcact ccgccgggcc gccccggctt cactttcggg cgggaggccg
     6001 aggaatggag cgaggatatg ccttcggaaa cgagccggct cgtcggctcg ggggaaggct
     6061 cgctcgacaa ggacggccgc ttggccgtca aggttcccct ggccgcggga aaggaaaccg
     6121 ggaccctcag cgccgacctg gaagtcaccg ttcaaagtcc cagccggcgg tccatttcca
     6181 gccgaatcca agcgctggtt catcccggtg atttctatct cggcttgaag ccgtccacct
     6241 cgtttctgaa aaagggggat cctctgtccg tccaagtcat cgccgccctc cccgacggga
     6301 cgctccaagg cggccgggcg gttaaagtca agcttctccg gcgtatctgg aaatcggcga
     6361 ggaaagccgg cttgggaggc cgcttggagt ggatttcgga gaaatccgat accgtggtcg
     6421 cggtccggga cgttaagacc gcggattccg agcccgccgc cgccgccttc gcgccggatc
     6481 aatcgggctt ttacttggtc gtcgctgaag ggcgtgacgg ctcgggccgg gcggtttcgt
     6541 catcgaccta cctgtatgtc a
//
LOCUS       DAOOXH010000114         6552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_13444_length_6552_cov_7.394644, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000114 DAOOXH010000000
VERSION     DAOOXH010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13444_length_6552_cov_7.394644"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..410)
                     /locus_tag="PKV64_10370"
     CDS             complement(129..410)
                     /locus_tag="PKV64_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17517.1"
                     /translation="MFSSDDDDPGNPLSVRRTLIPLNIEDAESPPSAGRRLWRIFRRK
                     KRRAATENEDFPRPPRPEPRPDARPENAGTPDNDFPESLDGENGKADQE"
     gene            559..933
                     /gene="rpoZ"
                     /locus_tag="PKV64_10375"
     CDS             559..933
                     /gene="rpoZ"
                     /locus_tag="PKV64_10375"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPN17518.1"
                     /translation="MELYGNVDSKFRYVIVAAKRAKQLIKGSKPKVTLKSRNPIRLAQ
                     AEVRAGLVDYHILQNTAEDVVDDSSLIHAGDVDEADDAGSVLADEGDLAGDDALVEAD
                     EEGAELDEDLAEVELVEEKDDE"
     gene            complement(943..1458)
                     /gene="cyaB"
                     /locus_tag="PKV64_10380"
     CDS             complement(943..1458)
                     /gene="cyaB"
                     /locus_tag="PKV64_10380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HPN17519.1"
                     /translation="MTEIEVKVRVDDLAAVKEKLLAMGAVVFKERHFEENTLYDFRDR
                     RLAGRREALRIRRVGRKAFLTFKGTPQKSRKFKIRTEYETEVHDGRSLVRILQSLGMI
                     EVFRYEKHRTVLRKGTLLVCLDETRLGGFVEFEGEREKIARTARALGMSKKDWITKSY
                     PALYREAEPGA"
     gene            complement(1462..2457)
                     /locus_tag="PKV64_10385"
     CDS             complement(1462..2457)
                     /locus_tag="PKV64_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPN17520.1"
                     /translation="MTETNDSARLLTELYAEIRPDLDRVEGRLSEMIRSDNPLVDEIN
                     AYLFAAGGKRLRPALLTLCARMTGNGAGPDAAFWSAVIEIIHAASLVHDDIVDRTDTR
                     RGRETVHSRWGANITVLLGDHLFIRAIHEALRTRRYEIIDVLSEATERMIEGELLECA
                     VSGDPLLTEDRYLEILAKKTAALFGAACRIGGILGQADPDLTRRLEEFGRNLGLAFQI
                     TDDLLDFTGRPDTLGKPVLTDLREGRITLPVIYALRKIDGPARTDFIARIAAREDDPS
                     AVEAIRDAVLAAGAIAEAAAAAAAFTAKAKSFLPDETPTARTLAALADFVCGRNV"
     gene            complement(2497..3279)
                     /locus_tag="PKV64_10390"
     CDS             complement(2497..3279)
                     /locus_tag="PKV64_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020408828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPN17521.1"
                     /translation="MDPISLPDSHCHLDLDEFAADREAVLKRAREAGVSAFLCPADVS
                     LPAGLQTVLELAARHPDITAAAGLHPHQAKHRRPDQAETLRALAADGKIAAVGEIGLD
                     YHYDFSTPAEQRAAFREQLALAGELGLPAVIHSRLAGADIRGAVDAEGFGRGGILHCF
                     TEEREIAEAMIDRGFLISFSGILTFPNAAALRDVARALPLDRILVETDAPYLAPVPHR
                     GKRNEPAFVLETARRLAALRGLPLEDLAAAVTANYRSLFPAR"
     gene            complement(3338..5107)
                     /locus_tag="PKV64_10395"
     CDS             complement(3338..5107)
                     /locus_tag="PKV64_10395"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HPN17522.1"
                     /translation="MKKALIASLIMGIFILGGTGFLSAQQTAEDAKFTKLVNSYLDEY
                     WKFYPTAATLAGFGKYDNKIEDFSESAIDKRTTAVDAVNKEMITKIAVDKLSPEMQLD
                     HRLMLDMLDYELFRLEYLDPQEYNPLFYNEIFLHGIRGLLVKNFSEAKLAAATERAKA
                     LPGFIKFAKENLKTPPAEYTQAAIDQMPAILAFFKNDVPGLIEKSGADARNKFNAELT
                     KLTAALEDWDRFLQNELMAKSTGNFRLGPEAHARLLRLTGQTSLMTEELVNRSKADYN
                     NIRREMALVCIPLYQIMYPNVDLNKINAGSEDANRNLVIKGVLDKIQGDHIAKDAWIG
                     KIKNAAGEIKSFIEKTNILDIPSANVSIDPMEPIYQGLTWLKFIPPTAYENGGDYKIQ
                     IQPVPASWPEDKVQSFLEEFNNFYLYFWTVERVYPGMFFPAAAAAAVPSSIVRKLAPF
                     AQPLLTGWPLYTEEMFIYAGFGEYDLRLRLNQLKLKLKAVIDFQMELNVHQGNFTKEQ
                     VIAYMTKGGFMTEAEAERKWQMLVLNPGYSFYPYAGYQEILDMEKDYKAAKGEAFNQK
                     EFLNKLTGFGPLPIRQIKAKMAQ"
     gene            complement(5258..6259)
                     /locus_tag="PKV64_10400"
     CDS             complement(5258..6259)
                     /locus_tag="PKV64_10400"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPN17523.1"
                     /translation="MSIRTVITGTGRYLPPKVVSNFDLEKIMNTTDDWIVQRSGIRTR
                     RYAEDGVGSMTLGLEAAKAALEDARCAPADIDFIVAATLSPDHYFPGIGVEIQAALGC
                     GPIGALDVRAQCSGFIYALSVADQFIRAGTYKKILVVASEVQSHNLDYSDAGRDMAVL
                     FGDGAGAVVCEPSTDGSGHALLSTHLFGDGRFSKILSLDVPSSKKNPTYTPEIFDQKL
                     YYPRMDGKRVFINAVERMPEAVRTALTANGLTIDDVRWLIPHQANDRISLMVAQNLKI
                     PVEKVVRNIEKYGNTTAASIPIALDETVKAGLLKADDIVILTAFGSGFTWASAALRW"
BASE COUNT         1096 a         1902 c         2273 g         1281 t
ORIGIN      
        1 cccccggggg gtttagaggt ttttgcgatg agaaagcaga cgttgctcgg atggatcgga
       61 ctcgcggccg ccgccctcgt cctcttcggc ttcaggtggc tccttcggcg ccggacgccg
      121 tcgttccatc attcctgatc cgccttcccg ttttccccgt ccaagctctc cggaaagtcg
      181 ttgtccggcg tcccggcgtt ttcgggccgg gcgtccggcc gcggctccgg cctcggcggg
      241 cgggggaaat cctcgttttc cgtcgcggct ctccgctttt tccggcggaa aatccgccac
      301 aggcgccggc cggcggaagg gggcgactcg gcgtcctcga tgttcagggg aatgagcgtc
      361 cgccggacgg aaagcgggtt gccgggatcg tcgtcgtcgg aggaaaacat gggatgaatt
      421 ccgccgtctc atcttagcgg aggacggcga aaaaaacaac ggccggtccg gccgccgggc
      481 gggcgtttga tttatcccta ggattttgct aaaattacac gcctcaaact agaaacgctt
      541 ccgaggagtg agacgtcttt ggaactatac ggcaacgtcg atagcaagtt tcgttacgtg
      601 atcgtcgccg ccaagcgggc gaagcagctg atcaagggct ccaagccgaa ggtgacgctc
      661 aaatcgcgga atcccatccg tctggcccaa gccgaggtcc gggcgggcct ggtcgattat
      721 cacatccttc agaataccgc cgaggacgtc gtcgacgact cctccctgat ccacgcgggc
      781 gacgtcgacg aggccgatga cgccggctcc gttctggccg atgaaggaga tctcgccgga
      841 gacgacgccc ttgtcgaggc cgacgaggaa ggggcggagc tcgacgagga tctggccgaa
      901 gtcgagctgg tcgaggaaaa agacgacgag tgaggccggg ccttaggctc ccggctccgc
      961 ttccctgtaa agcgccgggt agctcttcgt aatccaatct ttcttggaca tccccaaggc
     1021 ccgggccgtc cgggcgatct tctcccgttc gccctcaaat tccacgaatc ctcccaagcg
     1081 ggtctcgtcc aggcagacaa gaagcgtccc cttgcgcagc accgtccggt gcttttcgta
     1141 gcggaagacc tcgatcattc ccagggattg gaggatccgg acgagcgacc ggccgtcgtg
     1201 gacctcggtt tcgtattccg tccggatctt gaacttgcgc gacttttggg gggttccttt
     1261 gaaggtcagg aaggccttgc ggccgacccg gcggatccgg agcgcttccc ggcgcccggc
     1321 cagccgccgg tcgcggaaat catagagcgt gttttcctcg aagtgccgtt ccttgaacac
     1381 cacggccccc atggccagga gcttctcctt gaccgcggcc aggtcgtcga cccggacttt
     1441 gacttcgatt tcggtcatct tttatacatt ccggccgcag acaaaatcgg ccagggcggc
     1501 cagcgtccgg gctgttgggg tttcgtcggg gagaaaggac ttggccttgg ccgtgaaggc
     1561 cgcggccgcg gcggccgcct cggcgatcgc cccggcggcg aggacggcgt cccggatcgc
     1621 ctcaaccgcg gacgggtcgt cctcccgggc cgcgatccgg gcgatgaaat ccgtccgggc
     1681 cgggccgtcg attttccgga gggcgtagat gaccggaagg gtgatccggc cctcgcgcag
     1741 gtcggtcagg acgggcttgc ccagcgtgtc cggccggccg gtgaaatcca ggaggtcgtc
     1801 ggtgatctgg aaggccaggc cgaggttccg gccgaattct tccaggcgcc gggtcaagtc
     1861 cggatcggcc tgtcccagga tccctccgat ccggcaggcg gcgccgaaca gggcggccgt
     1921 ttttttagcg aggatctcca agtaacggtc ctcggttaga aggggatcgc cgctgacggc
     1981 gcattccagg agctcgccct cgatcatccg ctccgtcgcc tccgagagca cgtcgatgat
     2041 ctcatagcgg cgggtccgca gggcttcgtg gatggcccgg atgaagagat gatcccccag
     2101 gagaacggtg atgttggccc cccaccggga atggaccgtc tcccgtccgc ggcgcgtgtc
     2161 cgtccggtcg acgatgtcgt cgtggacgag gctggccgcg tggatgatct cgatcacggc
     2221 cgaccagaag gcggcgtccg ggccggcccc gttgccggtc atccgggcgc agagggtgag
     2281 gagggcgggg cggagcctct tccccccggc ggcgaagagg taggcgttga tctcgtcgac
     2341 gaggggattg tcggagcgga tcatctcgga cagccggcct tcgacgcggt cgagatccgg
     2401 ccggatctcg gcgtacagct cggtgagcag gcgggcggaa tcgttcgttt cggtcatcgg
     2461 aatcgcggct tatcttagca tggggggggc cgcccatcaa cgggcgggaa acagggaccg
     2521 gtaattcgcc gtcacggcgg cggccaggtc ctccaggggg aggccgcgga gggcggccag
     2581 ccgccgggcg gtctcgagga cgaaggcggg ctcgttgcgc tttccccggt gggggacggg
     2641 cgccaggtag ggggcgtcgg tctcgacgag gatccggtcg aggggcaggg cccgggcgac
     2701 gtcccggagg gccgcggcgt tgggaaacgt caggatcccg gaaaaggaga tgaggaagcc
     2761 gcggtcgatc atcgcctcgg cgatctcccg ctcctcggtg aagcagtgga gaatgccgcc
     2821 gcggccgaag ccctcggcgt cgacggcccc gcggatatcc gccccggcca ggcggctgtg
     2881 gatgacggcc ggaaggccga gctcgccggc cagggcgagt tgttcccgga aggcggcccg
     2941 ctgctcggcc ggcgtcgaga aatcgtagtg gtagtcgagg ccgatttcgc ccacggccgc
     3001 gatttttccg tccgcggcca gggcccggag cgtctcggct tggtccggcc ggcggtgctt
     3061 ggcttggtgg ggatggagcc cggccgcggc cgtgatgtcc ggatgccggg cggccagctc
     3121 gaggaccgtt tggagcccgg ccggaaggga gacgtcggcc gggcagagga aagccgagac
     3181 gccggcctcc cgggcccgct tgaggacggc ctcgcggtcg gccgcgaatt cgtcgaggtc
     3241 gaggtggcag tggctgtcgg gaagggagat cgggtccatg ggggtgaaaa aaaatccccc
     3301 ggctccgcgg acggaaccgg gggatggcga ggcgggatta ctgggccatc ttcgccttga
     3361 tctgccggat cggcaggggg ccgaaaccgg tcagcttgtt caggaattct ttctggttga
     3421 aggcttcgcc cttggccgct ttgtaatctt tctccatgtc caggatttcc tggtagccgg
     3481 cgtagggata gaagctgtag cccgggttga ggacgagcat ctgccatttc cgctcggcct
     3541 cggcctcggt catgaagccg cccttggtca tgtaggcgat gacctgttcc ttggtgaaat
     3601 tgccctggtg gacgttgagc tccatctgga agtcgatgac cgctttgagc ttcagcttga
     3661 gctggttcaa gcgcagccgg agatcgtact cgccgaaccc ggcgtagatg aacatctcct
     3721 cggtatagag cggccagccg gtcaggaggg gctgggcgaa gggggcgagc ttccggacga
     3781 tggaggaggg gacggcggcg gccgccgcgg ccgggaagaa catcccggga tagacgcgct
     3841 cgaccgtcca gaagtacaag tagaagttgt tgaattcttc gaggaaggat tgaaccttgt
     3901 cctccggcca ggaggccggg acgggctgga tctggatttt gtagtcgccg ccgttttcat
     3961 acgcggtcgg ggggatgaac ttcagccagg tcaggccctg gtagatgggc tccatgggat
     4021 cgatggagac gttcgccgag gggatatcca ggatattcgt cttttcgatg aaggatttga
     4081 tttccccggc ggcgtttttg atcttcccga tccaggcgtc cttggcgatg tggtcgccct
     4141 ggatcttgtc cagaacgccc ttgatgacca ggttccggtt ggcgtcctcg ctgccggcgt
     4201 tgattttgtt gaggtcgacg ttgggataca tgatctggta cagcgggatg cagaccaggg
     4261 ccatttcccg tcggatgttg ttgtagtcgg ccttggagcg gttgacgagc tcctcggtca
     4321 tgagggaggt ctggccggtc aggcggagca gccgggcgtg agcctcgggg ccgagccgga
     4381 aattcccggt ggatttcgcc atgagctcgt tctgcaggaa ccggtcccaa tcctccaggg
     4441 cggccgtgag cttggtcagc tcggcgttga atttgttccg ggcgtcggcg ccggacttct
     4501 cgatgaggcc ggggacgtcg ttcttgaaga aggcgaggat cgccggcatc tgatcgatgg
     4561 ccgcttgggt gtactccgcc ggcggcgttt tgaggttttc cttggcgaac ttgatgaagc
     4621 cgggcagggc cttggcccgc tcggtcgcgg ccgccagctt cgcctcggag aaattcttca
     4681 ccaggagccc ccgaatgccg tgaaggaaga tctcgttgta aaacagggga ttgtattcct
     4741 ggggatccag gtattccagc cggaacagct cgtaatcgag catgtcgagc atcagacggt
     4801 ggtcgagctg catctccggg ctgagcttgt cgacggcgat cttcgtgatc atttccttgt
     4861 tgaccgcgtc gacggccgtg gtccgcttgt cgatggcgga ctcggaaaaa tcctcgatct
     4921 tattgtcgta tttgccgaac ccggccaggg tggccgccgt cgggtaaaac ttccaatatt
     4981 catcgaggta ggaattcacg agcttggtga acttggcgtc ctccgccgtt tgctgggcgg
     5041 agagaaagcc ggttccgcca aggatgaaga ttcccatgat gagggaagcg atcagcgcct
     5101 ttttcatctt cctcctcctt cttcgtaaag attttttaaa ggcaaatcct agcagacttg
     5161 cccccggatt gcaattataa agagcttttt acatttttta attttcgggg cgcgccgttt
     5221 catcttctct gttttaaaac aagcaaggat cgtgccatca ccaccggagg gcggccgagg
     5281 cccaggtgaa tcccgagccg aaggcggtga gaatcacgat gtcgtccgcc ttcaggagcc
     5341 cggctttgac cgtctcgtcc agggcgatgg gaatcgaagc ggccgtcgtg ttcccgtact
     5401 tctcgatgtt gcggacgacc ttttcgacgg gaatcttgag attctgggcg accatgaggg
     5461 aaatgcggtc gttggcctgg tgggggatga gccagcggac gtcgtcgatc gtcaggccgt
     5521 tggcggtcag ggccgtccgg accgcctccg gcatccgctc gacggcgttg atgaagaccc
     5581 gcttgccgtc catccgcggg tagtagagct tttgatcgaa gatctcgggc gtgtaagtcg
     5641 gattcttctt ggagctcggc acgtccaggg aaaggatctt ggagaaccgt ccgtcgccga
     5701 agagatgagt ggagagaagg gcgtggcccg agccgtccgt cgacggctcg cagacgaccg
     5761 ccccggcccc gtcgccgaag aggacggcca tgtcccggcc ggcgtcggaa tagtccaggt
     5821 tgtgggactg aacctcggag gcgacgacga ggattttctt gtacgtcccg gcccggatga
     5881 actggtcggc gaccgagagg gcgtagatga agccgctgca ctgggcccgg acgtcgaggg
     5941 ccccgatagg gccgcagccc agggcggcct ggatttcgac cccgatgccc gggaagtaat
     6001 gatcggggga gagggtggcg gcgacgatga aatcgatgtc ggccggcgcg caccgggcgt
     6061 cctcgagggc cgccttggcc gcctccaggc ccagggtcat gcttccgacg ccgtcctcgg
     6121 cgtagcggcg ggtccggatg ccggagcgct ggacgatcca atcgtccgtc gtgttcatga
     6181 ttttttccag gtcgaagttg gagacgacct tgggcgggag gtagcggccg gttccggtga
     6241 tgaccgttcg aatgctcatg tcttgaacct cacctagcaa gtgacgtcat tatattgaag
     6301 cggctccggt tttgcaaaga cgggattgac acggacgcat ggggctttta aactgtccgg
     6361 cgggtgcttc ccttatgaga aaactacgat ttctcgcgat cgccgccgcc ctggccctgg
     6421 cggggggaat tccaggggcg gccgccgagg accttattaa aatagaagcc ggcatcgccc
     6481 cccggcggct ggcccggggc cgggaaggga aggtcgtcct caggatcagc ttgaggaaag
     6541 ggatcgccgt ct
//
LOCUS       DAOOXH010000115         6538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_13487_length_6538_cov_6.701064, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000115 DAOOXH010000000
VERSION     DAOOXH010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13487_length_6538_cov_6.701064"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PKV64_10405"
     CDS             <1..985
                     /locus_tag="PKV64_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPN17524.1"
                     /translation="IHKFVSTGAVINIPAIFIVLLLTGLLVRGIKESSRVNMIIVIVK
                     IAVIVFFIVLAVWHIKPSNWTPFMPFGFGGVMTAAAIVFLAFVGFDAISTAAEEAKNP
                     QRDMPRAIMSSLAVATILYMAVSAIMTGTVPYDKLGVPDPVALVLNELRMPWASSIVS
                     VGALAGITSVLLVLILGQPRILFAMSRDGLLPPALSHVHKKYKTPHKTTILTGGIVAV
                     AAGLLPIQVVAELCSIGTLFAFIIVCSGVLVLRYTRRDIHRPFKTPLFPFVPAAGVLL
                     CGYLMINLPRTAWERFLIWLIIGLAIYFSYSRRNSGLRREPSEQIEADIKI"
     gene            complement(1035..1655)
                     /locus_tag="PKV64_10410"
     CDS             complement(1035..1655)
                     /locus_tag="PKV64_10410"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HPN17525.1"
                     /translation="MKKAWLLAAVILAGAGCFAAHAKRYFDLGVPVSGPAGPAFDRTL
                     FLDRPVVEGIYDDFRIVYRISPTEINYYAYNFWAERPSRLVRDMIRDRLVAGRRFRRI
                     EMEPGKEPADWTLRIKIFRIEEEDRVDRWYGRLAMSLEVVDPVSGALIAARTFDRLEP
                     LAEKDAGLLAGVISEILAEELDAFLGELAAKLIDKTGDHTPFPLFL"
     gene            complement(1655..2599)
                     /locus_tag="PKV64_10415"
     CDS             complement(1655..2599)
                     /locus_tag="PKV64_10415"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPN17526.1"
                     /translation="MLTKEQKTRLGIFWVVSVALLLFLAGMLIVPAFRNKGLPYTINI
                     KGASVNGLSVGASVKYQGVDVGKVREIRVNPDDLSSILVEIGIVREFPVKQDMTATLT
                     ITGITGQKFIELSGGSSDSPLLAPGGEIPPARGLGERAEDIVANIEGAIKSVNELLNA
                     ENRAKIDQVLTAWHRSTEVVSRVLESKEMNLTGAIDNIEKASLEFGEVTENLRKITED
                     LSALTGTLDAKGGSILDNINQRFSEEEMGKVLTNLESFIAEANASLKTIENVLMIQQS
                     DLRRTVENLGVAVDNLSALARTLAEDPMMLIRSKKEKK"
     gene            complement(2589..3335)
                     /locus_tag="PKV64_10420"
     CDS             complement(2589..3335)
                     /locus_tag="PKV64_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPN17527.1"
                     /translation="MENIRAVDLDVGYGRETILRNLNFTLPPGRITVIVGESGSGKST
                     LLKTLIGLTRPLRGDVYWGEERIDYDSERILGALYRRIGVLYQNNALLNSLTLYENVA
                     LPIRIHRPETPRAVEADRVREVLGMVGLVGADHKYPFELSGGMRKRGALARSMVLGPE
                     IVFCDEPSAGLDPLTALGLDDLLRSLQRSRGVTFVAVTHELRSIGRIADRILLLRGDG
                     IHFDGTLAEMEASEDEYLRSFFLRTDRHAD"
     gene            complement(3342..4091)
                     /locus_tag="PKV64_10425"
     CDS             complement(3342..4091)
                     /locus_tag="PKV64_10425"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN17528.1"
                     /translation="MRLKEEFRRFFSLLADELHYTAQYLGSRRGVLPGSVVQQFYVMG
                     YKSFPIVALITFLVGVTISLTSAAQLRLFGADIYLADLIGLAMIKELVPLMTGIMLAG
                     KVGASITAEISTMKVMDEIDALRTMGIVPEKFLMAPRLIAITLVVPLLVAMADTVGVA
                     GGVLVAQVALGTPPAVFLREVFDIVTLSDFLVGIVKTLVFGWAVVVGAGFKGFFIERG
                     AEGVGKATTESVVLAISLIIGLDCVFAFILY"
     gene            4191..5516
                     /locus_tag="PKV64_10430"
     CDS             4191..5516
                     /locus_tag="PKV64_10430"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPN17529.1"
                     /translation="MKKAVAAGAVILFAGAALLSASFRKEDIKVHRLDNGLKILLLED
                     HTIPNIALYTYYRVGSRNERPGLTGVSHFIEHMMFNGSPKVPPGEFDRRMEFAGGSNN
                     ASTSQDRTGYTDWIPAAALEEMLEMEADRMQDATFDPEVLESERQVIASERRMGVDND
                     NDAILSENVQATAIMAHPYHWDVIGWMSDIQGWRRDEIIAYYKTYYAPNNAVLILVGE
                     FAEARALELIRKYFGPIPSGPTPPAVVTVEPPQMGPKRVFIQRDAQVPSFQAVWHAPA
                     VHDPGSIPMDILGRVLLRGESSRLYRRLVRQEQLASSVFGGASRTIDPYLFAVHVKPK
                     PGADLARIEAVIEEELAKIKKEGLADSEIQKVLNAVRSDFYRSLQTIAGKAYQLAETE
                     TAYGGFERMFTLMDDYAAVTNAKIIEAANQYFTDNNKTVGTLVPEGGRK"
     gene            5513..>6538
                     /locus_tag="PKV64_10435"
     CDS             5513..>6538
                     /locus_tag="PKV64_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPN17530.1"
                     /translation="MKSRMSLLCLSAAVFLAASAAGAAFKLPVPEKLTLKNGIVVYYL
                     KTAEVPMVSFRLYLKGAGSAQEPSAFEGIAGMTADQIMKGTPAMGAEAVAEALDFLGA
                     RAGVSAADEYASLSAECLSEHFPRVLDIAAACLKEPAFLEDEFKKARDIRLNELKSSK
                     DNPGRAVRYYFLKAYFGTHPLGHFPSGTDESLVKMTAADVRDYYRTYYGPKGAIGALV
                     GDIEKKQALVLLNAALGGWKGANVPVTATPPLPEPRGAKLLLVDKPDATQAYWILGAP
                     GHALGDKITPQAAVMNTLFGGRFTSWLSTEMRIKRGLTYGANSSLQPYRIGGKPRGEA
                     ALDPHFGG"
BASE COUNT         1254 a         2176 c         1928 g         1180 t
ORIGIN      
        1 gattcataag ttcgtttcca ccggggcggt catcaacatt ccggcgatct tcatcgtcct
       61 cctcctgacg ggtcttctcg tccgggggat caaggagagc tcgcgggtca acatgatcat
      121 cgtcatcgtc aagatcgcgg tcatcgtctt cttcatcgtc ctggccgtct ggcatatcaa
      181 gccgtccaac tggacgccct tcatgccctt cggcttcggc ggggtgatga cggccgcggc
      241 gatcgtcttc ctggctttcg tcggattcga cgcgatctcc acggcggccg aggaggccaa
      301 gaacccccag cgggacatgc cccgcgcgat catgagctcg ctggccgtgg ccacgatcct
      361 gtacatggcc gtgtcggcca tcatgaccgg aacggtcccc tatgataagc tcggcgtccc
      421 cgatcccgtg gctctcgttc tcaacgagct gcggatgccc tgggcctcat ccatcgtcag
      481 cgtcggagcc ctggccggca tcaccagcgt cctgctcgtc ctcatcctcg gccagccccg
      541 catcctgttc gccatgtccc gcgacggctt gctcccgccg gccctgtccc atgtccacaa
      601 gaagtacaaa acgccccata aaacgacgat cctgacgggc ggcatcgtcg ccgtcgccgc
      661 cggcctcctg cccatccagg tcgtcgccga gctgtgctcg atcgggaccc tcttcgcctt
      721 catcatcgtc tgctcgggcg tcctcgttct ccggtacacg cggagggaca tccaccggcc
      781 gttcaaaacg cctctcttcc ccttcgtccc ggccgccggg gtgctcctct gcggctattt
      841 aatgatcaac ttgccgagaa ccgcttggga gcggttcctg atctggctga tcatcggact
      901 ggcgatttat ttctcctaca gccgccgcaa cagcgggctt cgccgcgaac cctcggaaca
      961 aatcgaagcc gacatcaaaa tctaatcgga gacgcggact ccttcccgat ttccggcgcg
     1021 cgagttattc ctccttagag gaaaagaggg aaaggagtat ggtctccggt tttatcgatt
     1081 aattttgcgg ccagctcgcc gagaaacgcg tcgagctcct cggccaggat ctccgagatc
     1141 accccggcca ggaggccggc gtctttctcc gccagcggct cgagccggtc aaaggtccgg
     1201 gcggcgatca gcgcgccgga gaccgggtcg accacttcca gggacatcgc cagccggccg
     1261 taccagcgat ccacccggtc ttcctcctcg atgcggaaaa tcttgatcct caaggtccag
     1321 tcggccggtt ccttccccgg ctccatctcg atcctccgga acctccgtcc cgcgacgagc
     1381 cggtcccgga tcatgtcccg aacaagccgg gagggccgtt cggcccaaaa gttataggcg
     1441 taatagttga tttccgtcgg cgagatccgg tagacgatcc ggaagtcgtc atagatcccc
     1501 tcgacgaccg gccggtcgag aaacaaagtc cggtcaaagg ccggcccggc cgggccggag
     1561 accgggaccc ccaggtcgaa ataacgcttg gcgtgagcgg cgaagcaacc cgcgccggcc
     1621 aggatgacgg cggcaagaag ccatgctttt ttcatcactt cttttccttt ttacttcgaa
     1681 taagcatcat gggatcctcg gccagggtcc gggcgagagc ggagaggtta tccacggcca
     1741 ctcccaggtt ctcgaccgtc cggcggaggt cggactgctg gatcatcaag acgttctcga
     1801 tcgtcttgag actcgcgttc gcttcggcga tgaacgactc gaggttggtc aggaccttac
     1861 ccatctcctc ctcggaaaag cgctgattga tgttgtcgag gatcgagccg cccttggcgt
     1921 ccagagtccc cgtcagggcg gacagatcct cggtgatctt ccggaggttc tcggtgacct
     1981 cgccgaactc gagggaggct ttctcgatat tgtcgatggc cccggtcaag ttcatctcct
     2041 tgctttcgag gacgcgggag accacttcgg tgctccggtg ccaggcggtc agaacctggt
     2101 cgatcttggc ccggttctcc gcgttgagga gctcgttgac gcttttgatc gctccctcga
     2161 tgttggccac gatgtcctcg gccctctccc ccaagccgcg ggccggaggg atctccccgc
     2221 cgggcgcgag aaggggggaa tccgaactcc cgccggaaag ctcgatgaac ttctgtccgg
     2281 tgatccccgt tatcgtcaac gtcgccgtca tgtcctgctt caccgggaac tcccggacga
     2341 tgccgatctc gacaaggatg gagctcaagt cgtcgggatt gacgcggatt tcccggacct
     2401 ttccgacatc cactccctga tacttgaccg aagcgccgac ggagaggccg ttgaccgatg
     2461 cccccttgat gttgatcgtg tagggcaggc ccttattccg gaaagccggg acaatgagca
     2521 tcccggccag aaaaagaagc agcgcgaccg agacgaccca aaagatcccc aagcgcgttt
     2581 tttgttcttt agtcagcatg acggtccgtc ctcaggaaga aagatcgcag atattcatcc
     2641 tccgacgcct ccatctccgc caacgtcccg tcgaaatgga tcccgtcgcc gcggaggagg
     2701 aggatgcggt cggcgatccg cccgatcgac cgcagctcgt gcgtcacggc gacgaacgtc
     2761 accccgcggg atctctggag cgagcggagg aggtcgtcca gccccaaggc ggtgagagga
     2821 tcaagaccgg ccgacggctc gtcgcagaag acgatctccg gcccgaggac catcgaccgg
     2881 gcgagggctc cccgcttccg cattccgccc gaaagctcga acgggtactt gtggtcggcc
     2941 ccgaccaagc cgaccatccc caggacctcg cggacgcggt cggcctcgac cgcccgggga
     3001 gtctcgggcc gatggatccg gatgggcagg gcgacattct cgtagagggt caaggaattc
     3061 agcagggcgt tgttttggta aaggacgccg atccgccgat acagggcgcc caggatgcgc
     3121 tcggagtcat agtcgatccg ctcctccccc cagtagacgt cgccgcgaag cggccgggtc
     3181 agcccgatca gggttttgag aagcgtcgat ttccccgatc cgctctcccc gacgatcacc
     3241 gtgatccggc cgggaggcag ggtgaaattc agattccgga gaatcgtctc tcttccgtac
     3301 ccgacgtcga ggtcgaccgc ccgtatgttt tccatgatct cttagtagag gatgaaggcg
     3361 aacacgcaat cgaggccgat gatcagggaa atggccagga cgaccgactc ggtcgtggcc
     3421 ttccccacgc cctcggcccc ccgctcgatg aagaagccct tgaatcccgc cccgacgacg
     3481 acggcccagc cgaaaacaag ggtcttgacg attcccacca ggaaatcgct caaggtcaca
     3541 atgtcgaaga cctcccggag gaagacggcc ggaggcgtgc ccaaggcgac ctgggcgacg
     3601 aggacgccgc cggccacgcc gacggtatcg gccatcgcca ccagaagcgg cacgaccagg
     3661 gtgatcgcga tcaggcgcgg ggccatcaga aacttctccg gaacgatgcc catcgtccgg
     3721 agggcgtcga tctcgtccat gaccttcatc gtcgaaatct cggccgtgat ggatgccccg
     3781 accttgcccg ccagcatgat cccggtcatg agcgggacaa gctccttgat catcgccagg
     3841 ccgataaggt cggccaggta gatgtccgcc ccgaagagcc gcagttgggc ggccgaggtg
     3901 agcgagatcg tgacgccgac aagaaacgtg atcagggcga cgatcggaaa ggacttgtag
     3961 cccatgacat agaactgctg aaccaccgat ccgggcaaaa ccccccgccg ggatccgagg
     4021 tactgggcgg tatagtggag ctcgtcggca aggagactga aaaagcgcct gaattcctcc
     4081 ttcagtctca taccgtctcc cgcattggaa ggtttattat accttaaatt gaccgaccgg
     4141 cgacgagtct gttacattgt cctggaaacg ccatccttcg gaggtccgct atgaaaaaag
     4201 ccgtcgccgc gggcgccgtg attctcttcg ccggcgccgc cctcctctcc gcctcgttcc
     4261 ggaaggagga catcaaagtc caccggctcg ataacgggct gaagatcctt ctcctcgagg
     4321 atcacacgat ccccaacatc gccctgtaca cgtattaccg cgtcgggtcg cgcaacgagc
     4381 ggcccggcct gaccggggtc tcccatttca tcgagcacat gatgttcaac ggctccccca
     4441 aggtcccgcc gggagagttc gaccggagga tggaattcgc cggcggctcg aacaacgcct
     4501 cgaccagcca ggaccggacg ggatacaccg attggattcc cgcggccgcc ttggaagaga
     4561 tgctcgagat ggaagccgac cggatgcagg acgcgacgtt cgatcccgag gtcctcgagt
     4621 cggagcgcca agtcatcgcc tccgagcggc ggatgggcgt ggacaacgac aacgacgcca
     4681 ttctctctga aaacgtccag gcgacggcca tcatggccca tccctaccac tgggacgtca
     4741 tcggctggat gagcgacatc cagggctggc ggcgggacga aatcatcgcc tattacaaga
     4801 cctattacgc ccccaacaac gccgtcctga tcctcgtcgg cgagttcgcc gaggcccggg
     4861 ccctcgagct gatccggaaa tacttcggac cgatcccgtc cggcccgacg ccgccggccg
     4921 tcgtcaccgt cgagccgccg cagatgggcc ccaagcgcgt gttcatccaa cgcgacgccc
     4981 aggtcccctc cttccaggcc gtttggcacg ccccggccgt ccatgatccc ggatccattc
     5041 ccatggacat cctgggtcgg gttctcctcc gcggtgaaag cagccgcctc taccggcgcc
     5101 tggtccggca ggagcagctg gcgagcagcg ttttcggcgg ggcgagccgg accatcgacc
     5161 cctatctctt cgccgtgcac gtcaagccca agccgggcgc cgacttggcc cggatcgagg
     5221 cggtgatcga ggaagagctg gccaagatca agaaggaagg cctggccgat tccgagatcc
     5281 aaaaggttct gaacgccgtc cggagcgatt tttaccggag cctccagacc atcgccggca
     5341 aggcttacca gctggccgag accgaaacag cctacggcgg attcgagaga atgttcaccc
     5401 tgatggacga ttacgcggcc gtcaccaacg ccaagatcat cgaggccgcc aatcaatact
     5461 tcaccgacaa caacaagacc gttggaacgc tcgttcccga aggaggccgg aaatgaaatc
     5521 ccggatgagc cttctctgcc tttcggccgc ggttttcctg gccgcctcgg cggccggagc
     5581 ggccttcaag ctgcccgtcc cggaaaagct caccttgaag aacgggatcg tcgtctatta
     5641 cctgaagacg gccgaagtcc ccatggtcag cttccgcctt tatctcaagg gcgccggctc
     5701 cgcccaggag ccctccgcct tcgaaggcat cgccgggatg acggccgacc agatcatgaa
     5761 gggaaccccg gccatgggcg ctgaagccgt ggccgaagcc ctcgatttcc tcggcgcccg
     5821 ggccggagtt tcggcggccg atgaatacgc ctccctcagc gccgagtgcc tgtcggagca
     5881 ttttcccaga gtcctggaca tcgccgcggc ctgcctgaag gaaccggcgt tcctggagga
     5941 cgagttcaaa aaagcccgcg acatccggct gaacgagctg aaaagcagca aggacaaccc
     6001 gggccgggcc gtccggtatt atttcctcaa agcctatttc ggaacgcatc cgctcggtca
     6061 tttcccctcg gggacggacg aatccctggt caagatgacc gcggccgacg tccgggatta
     6121 ttaccggacg tattacgggc ccaagggagc gatcggggcc ctggtcggcg atatcgaaaa
     6181 gaagcaggcc ctcgtccttc tcaacgccgc cctcggcggc tggaagggag ctaacgtccc
     6241 ggtcacggcg acccctcccc tccccgagcc gcgcggggcg aagcttcttc tcgtcgacaa
     6301 gcccgacgcc acccaggctt actggatcct gggcgctccc ggccacgccc tgggggacaa
     6361 gatcactccg caggcggcgg tcatgaacac gctcttcggc ggccgcttca cctcttggct
     6421 ttccaccgaa atgcggatca agcgcggctt gacctatggg gcgaacagca gcctccaacc
     6481 ctaccggatc ggcggaaagc caagaggtga agccgcgctt gatccgcatt tcggtgga
//
LOCUS       DAOOXH010000116         6377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_13994_length_6377_cov_6.947960, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000116 DAOOXH010000000
VERSION     DAOOXH010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13994_length_6377_cov_6.947960"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..791)
                     /locus_tag="PKV64_10440"
     CDS             complement(144..791)
                     /locus_tag="PKV64_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HPN17531.1"
                     /translation="MAFINYTTNQLTIKIVYYGTGLSGKTTNLRYIQANIDRSSRSEL
                     ICLETETERTFFFDLLPVRAGYIGNFKATFQLMTVPGQVYYEASRKNVLVGTDGLVFV
                     ADSQIPLLDANMESLESLRRNFKEQHTELAAFPLVFQYNKRDLSDLIPVETLNNLLNP
                     KGAPFIEAAAITGLGVFETLKEIARMTVPRVREKIFGFEGAEYSRIGQKDELDLI"
     gene            complement(812..1978)
                     /locus_tag="PKV64_10445"
     CDS             complement(812..1978)
                     /locus_tag="PKV64_10445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPN17532.1"
                     /translation="MKIAFVVQRYGKEVMGGSELHCRMVAERLARRGHDCTVYTTAAK
                     DYVTWKNEYPEGEFILNGVVVKRYRVERPRDIASFNAYSDWIFANPHTAEDELRWMER
                     QGPFSLDLILALEREESLHDRFIFFTYLYYNTFWGLRMLKGKSLLVPTAHDEPALRLD
                     LMKEVFSRPAGFVFNTAAERAMLASRFSFEGKVQDIVGVGVDIPDPAGYREFLPKYQA
                     LAPYLLYAGRIEPGKGCAELFEYFQRARRRVPGLNLVLIGKQLMELPPDPRIRYLGFV
                     SPEEKNAAMAGALATVHPSHFESLCMAVLESLAVQTPVLVQEGSDPLKQHCLDSNGGL
                     FFSDASTFAAALGLLAGGGERLRAALGENGLRYVQKQYTWACVMEKYDILLGVL"
     gene            complement(1975..3048)
                     /locus_tag="PKV64_10450"
     CDS             complement(1975..3048)
                     /locus_tag="PKV64_10450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN17533.1"
                     /translation="MRVDQLVPAFHRGDAIGDEAWELRRFFRAEGCESEIYCLSRDRG
                     LENDSRLFESFPRPSASDVTILHYALPSPLSAALAGLPSRKALIYHNITPASFFEGVN
                     PEFARVARLGREELASLAPAVDVSLADSEYNRLELEALGFRDTHVFPLFVDFARYNRP
                     ENALLRDLYRDDRTNILFVGRIAPNKRIEHLVKTVFYYKKYISPLVRLMVVGKTNTFP
                     AYFAAVSRMADEFYLKPEEVLFFGHIPDDEMYALYRASDVFLSLSEHEGFCLPLVESM
                     IFDLPVVALAAAAVPYTLGGAGLLLREFRPDRTAETVHQAAKDPALRVQLIESGRREL
                     DRLKRFPREEFLRERIPGLGRKK"
     gene            complement(3045..4112)
                     /locus_tag="PKV64_10455"
     CDS             complement(3045..4112)
                     /locus_tag="PKV64_10455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPN17534.1"
                     /translation="MDVHQFATSLTYGDAISDEMLEIQSVLREHGFGSEIFVRQFDPR
                     SAAAVRDYREYPRWSKPENVVIFHFSIGSPVSKMFLRIPDRKMMIYHNITPAGFFLDS
                     HRILTRECYKGRLEINMFVDKVDLALGDSDFNRRELASVGYKKTGVLPILMNFRKFDG
                     PGDPVVRGLYETKKTTILYVGRVIPNKKFEDVVKAFAFYKKHFNPEARLILAGDYRGQ
                     DRYLAGLQDLIAKLRIADVHLTGHVEFPELLAYYRLADYYLSMSEHEGFGVPLLEAFH
                     LGIPVVAFAAGAVEETMNGGGVVLRRKDPLRTAGLIDEIERRPELRRKIVEGQKKALK
                     PYLRENVSRVLLAQIAEAAGR"
     gene            complement(4112..5287)
                     /locus_tag="PKV64_10460"
     CDS             complement(4112..5287)
                     /locus_tag="PKV64_10460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPN17535.1"
                     /translation="MNVAFVVQRYGLEINGGAELHCRWVAEHMSRHWNVEVLTTRAHD
                     YVTWADHYPEGEETINGIRVRRFSVPRPRDPERFGRLQSFILEEEHTERDELAWLEEE
                     GPTSPDLLDFIRRREADYDYFIFFSYRYWHSYWGIRSVPRKAILVPTAEHDAVIDLKF
                     FKDLFRLPRAFIYNSEEEREMIQAVSGNRSVPGLVVGVGTEVPAGAEPERFRRTQGID
                     RPYLLYLGRIDQNKGFPQLFDYFLQFKKDTGSDAGLVLIGNSILPIPDHPDIFALGFQ
                     PEQDKFDALAGAEALIMPSFFESLSMVTLEAWALRRPVLANARCEVLQGQCRRSDGGL
                     FYGNYPEFREALKLLLASAGLRRTLGENGRRYFETHYTWDIIEEKYLETVRCLEEEP"
     gene            complement(5284..5931)
                     /locus_tag="PKV64_10465"
     CDS             complement(5284..5931)
                     /locus_tag="PKV64_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17536.1"
                     /translation="MDDVKPAGKETIEGIEIDYDHFDVASIMAQVKRAAARRPVEPLE
                     AMPEPPAAPPAAPEPPAPAPSVPPPSKPGLKEIVKLKLSRAIRPFAPVIRLLGLPLHE
                     EILASWAEIGRISRRQDELSDRLDRALEKIDRRFSDSAADLDRLRDWLDVRLVDLDRS
                     MEYIKLLHNLDHNLVVELTKLRIEFETLKSRARILEKDLEAQTRRERVLEKRLGS"
     gene            complement(5955..6167)
                     /locus_tag="PKV64_10470"
     CDS             complement(5955..6167)
                     /locus_tag="PKV64_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17537.1"
                     /translation="MSDFLTIKCLDKAETAKIEREIEARIEARKKEGLLTDREVREIE
                     EMPLRPLPDILDVQAVYKNHLYPKKD"
BASE COUNT         1138 a         1933 c         2130 g         1176 t
ORIGIN      
        1 cggctccgcc ctccagggga tcgaggccga tgtcgtcgag gtcgaggtcg acatcgacga
       61 cataggcctc gatcccctgg agggcggagc tggacgtttt gatgagcatg gcgccatggg
      121 aagagacggc gccggaggga gatttatatc agatcgagct cgtctttctg gccgatccgg
      181 gaatattccg cgccctcgaa cccgaaaatc ttctcccgga cccggggaac ggtcatccgg
      241 gcgatttcct tgagcgtctc gaagacgccg agtccggtga tcgcggccgc ctcgatgaac
      301 ggcgccccct tgggattgag gaggttgttg agcgtctcga ccgggatcag gtcggagagg
      361 tcgcgcttgt tgtattggaa gaccaggggg aaggcggcca gctcggtgtg ctgctccttg
      421 aaattccggc ggaggctttc caggctttcc atgttggcgt cgaggagcgg gatctgggag
      481 tcggccacga agaccaggcc gtccgtcccc acgaggacgt ttttccgcga ggcctcgtag
      541 taaacctgtc cgggcacggt catcagctgg aaagtggcct tgaaattccc gatatacccg
      601 gcccggaccg gcagtagatc aaaaaagaaa gtccgctcgg tctcggtctc caggcagatc
      661 agctcgcttc gcgaggaccg atcgatattg gcttggatgt agcggagatt ggtcgttttt
      721 cccgacaaac ccgtcccgta gtaaacgatc ttgatcgtca gttggtttgt cgtgtagttg
      781 atgaatgcca tttcttcggg cgctcttcgg actacaacac ccctaagagt atatcatatt
      841 tttccatgac acacgcccaa gtatattgct tttgaacgta ccggaggccg ttttcaccca
      901 aagcggcccg gaggcgctcc ccgccccccg ccagcaggcc cagggcggcc gcgaacgtgg
      961 acgcgtcgga gaaaaacagc ccgccgttgc tgtccaggca gtgctgtttg agaggatcgg
     1021 atccttcctg gacgaggacc ggcgtctgga cggccaggga ctcgagaaca gccatgcaga
     1081 ggctttcgaa atgcgaggga tggacggtcg ccagggcccc ggccatggcc gcgttttttt
     1141 cctcggggga gacgaatccc aggtagcgga tcctcggatc gggaggaagc tccatgagct
     1201 gcttgccgat caggacgagg ttcaggccgg gaacccgccg ccgcgcccgc tggaaatatt
     1261 caaacagctc ggcgcagccc ttgcccggct cgatccggcc ggcgtagagg agatacggcg
     1321 cgagggcttg gtacttgggg aggaattccc ggtatccggc cgggtcgggg atgtccaccc
     1381 ccacgccgac aatgtcctgg accttgccct cgaaggagaa gcgggaggcc agcatcgccc
     1441 gctcggccgc ggtgttgaag acgaatcccg ccggccggga gaaaacctcc ttcatcaggt
     1501 ccagccggag ggccggctcg tcgtgggccg tcgggaccag gagcgatttt cccttgagca
     1561 tccgcaggcc ccagaaggtg ttgtagtaga ggtaggtgaa gaagatgaac cggtcgtgga
     1621 gggactcctc ccgctcgagg gcgaggatga gatcgaggct gaacggcccc tgccgctcca
     1681 tccaccgcag ctcgtcctcg gcggtatggg gattggcgaa aatccagtcc gagtaggcgt
     1741 tgaacgaggc gatgtcccgc ggccgctcga cccggtatcg cttgacgacg acgccgttga
     1801 ggatgaattc cccttccggg tactcgtttt tccaggtgac ataatccttg gccgccgtgg
     1861 tgtagaccgt gcagtcgtgg ccccgccggg ccagccgctc ggccaccatc cggcagtgaa
     1921 gctccgagcc ccccatcact tccttgccgt atcgctggac gacgaaggcg atcttcattt
     1981 ctttctcccg agccccggga tccgttcgcg gaggaactcc tcccggggga accgcttcag
     2041 ccggtcgagc tcccggcgtc cgctttcgat cagctggacc cggagggctg gatccttggc
     2101 cgcttggtga accgtttcgg ccgtccggtc gggccggaat tcccggagca gcagccccgc
     2161 cccgccgagg gtgtacggca cggccgcggc cgccagggcc acgaccggca ggtcgaaaat
     2221 catgctctcg acgagcggaa ggcagaaccc ctcgtgctcg ctcagggaga ggaagacgtc
     2281 ggacgcccgg tagagggcgt acatctcgtc gtccgggata tggccgaaga acaggacttc
     2341 ctcgggcttg agatagaact cgtcggccat ccgggagacg gccgcgaaat aggccggaaa
     2401 cgtgttggtc ttcccgacga ccatcagccg gacgagcggc gagatgtatt tcttgtagta
     2461 gaacaccgtc ttgacgaggt gttcgattct tttgttgggg gcgatccggc cgacaaaaag
     2521 gatgttcgtc cggtcgtccc ggtagagatc gcggaggagg gcgttttccg ggcggttgta
     2581 cctcgcgaaa tcgacgaaca gcgggaagac atgggtgtcg cggaagccca gggcctccag
     2641 ctccagccgg ttgtactcgg aatcggccaa gctcacgtcc acggccgggg ccagcgaggc
     2701 cagctcctcc cgcccgagcc gggcgacccg ggcgaattcc ggattgacgc cctcgaaaaa
     2761 cgaggccggg gtgatgttat ggtagatgag ggccttgcgc gagggcaggc cggccagggc
     2821 ggccgaaagc ggcgagggca gggcgtaatg gaggatggtc acgtccgagg cggacgggcg
     2881 ggggaacgat tcgaagagcc gggagtcgtt ttccaggccg cggtcgcggc tcaggcagta
     2941 gatctcgctt tcgcagccct ccgcccggaa aaaccggcgc agctcccagg cttcgtcgcc
     3001 gatcgcgtcg ccgcgatgga aggcggggac gagctggtcg actctcatct cccggccgcc
     3061 tcggcgatct gggccaggag gacgcggctg acgttctccc ggagatacgg cttcagggct
     3121 ttcttctggc cctcgacgat tttgcggcgg agctccggcc ggcgctcgat ctcgtcgatc
     3181 agcccggccg tccggagggg gtccttccgc cggaggacga cgccgccgcc gttcatggtc
     3241 tcctcgaccg ccccggcggc gaaagccacg accgggatgc cgaggtggaa cgcttccagg
     3301 aggggcacgc cgaagccttc gtgctcgctc atgctcaggt aataatcggc cagccggtaa
     3361 taagcgagca attcggggaa ttccacgtgg ccggtcaagt ggacgtcggc gatccggagc
     3421 ttggcgatca ggtcctggag gccggccagg taacggtcct ggccccggta gtccccggcc
     3481 aggatcagcc gggcctccgg gttgaaatgc tttttataaa aggcgaaggc cttgaccacg
     3541 tcctcgaact tcttgttcgg aatcacccgg ccgacgtaga ggatcgtcgt cttcttcgtt
     3601 tcgtacagcc cccggacgac gggatcgccg ggcccgtcga atttccggaa attcatcagg
     3661 atcggcagga cgccggtctt cttgtacccc acggaggcca gctcccggcg gttgaaatcg
     3721 gagtcgccca gggccaggtc gaccttgtcg acgaacatgt tgatttccag ccggcccttg
     3781 tagcattccc gggtcaggat tcggtgggaa tcgagaaaga acccggccgg ggtgatgttg
     3841 tggtagatca tcatcttgcg gtcggggatg cggagaaaca tcttgctgac gggggagccg
     3901 atcgagaaat ggaagatgac cacgttctcc ggcttgctcc agcggggata ttcccggtag
     3961 tcgcggacgg ccgcggccga gcgcggatcg aactgccgga cgaaaatctc cgagccgaag
     4021 ccgtgctccc ggagaacgga ctggatttcc agcatctcgt cggaaatggc gtcgccgtac
     4081 gtcagggacg tggcgaactg gtgcacgtcc atcagggctc ctcctcgaga cagcggacgg
     4141 tttcgaggta tttctcctcg atgatgtccc acgtgtagtg agtttcgaaa tagcggcggc
     4201 cgttttcccc cagggtccgg cggaggccgg ccgaggccag gagcagcttc agcgcctccc
     4261 gaaactcggg gtaatttccg tagaacaggc cgccgtcgga ccggcggcac tggccctgga
     4321 ggacctcgca gcgggcgttg gccaggaccg ggcgcctgag agcccaggcc tcgagggtga
     4381 ccatggacag gctttcaaag aacgagggca tgatcagggc ttccgccccg gccagggcgt
     4441 cgaacttgtc ctgctcgggc tgaaagccga gggcgaagat gtccggatgg tccgggatgg
     4501 gcaggatcga gttgccgatc aagacaaggc ccgcgtccga gccggtgtcc ttcttgaact
     4561 ggaggaaata atcaaacagc tggggaaagc ctttgttctg atcgatccgc ccgaggtaaa
     4621 ggaggtacgg tcgatcgatc ccttgggttc tccggaagcg ctccggctcg gcgccggccg
     4681 ggacttccgt cccgacgccg acgacgaggc cggggacgga acggttcccg gagacggcct
     4741 ggatcatctc ccgctcttcc tccgagttat agatgaaggc ccggggcagg cggaaaagat
     4801 ccttgaagaa cttcaagtcg atgaccgcgt cgtgctcggc cgtgggcacc aggatcgcct
     4861 tccgggggac cgaccggatg ccccagtagg aatgccagta gcggtagctg aagaagatga
     4921 aatagtcgta gtcggcctcg cgccggcgga tgaaatccag gagatcgggg gacgtcggcc
     4981 cctcctcctc gagccaggcc agctcgtccc gctcggtgtg ctcctcttcc aggatgaagc
     5041 tctgcagccg cccgaaccgc tcgggatcgc gcggccgggg cacggaaaag cgccggaccc
     5101 ggatcccgtt gatcgtctcc tccccctccg ggtagtgatc ggcccaggtg acgtagtcgt
     5161 gagcccgggt ggtcaggacc tcgacgttcc agtgccggga catgtgctcg gcgacccagc
     5221 ggcagtggag ttcggccccg ccgttgatct cgaggccgta gcgctgaacg acgaaggcga
     5281 cgttcatgaa cccagccgct tctccaggac ccgttcccga cgggtctggg cctccaggtc
     5341 cttctccagg atccgggccc ggctcttcag ggtctcgaac tcgatccgga gcttggtcag
     5401 ctcgacgacg aggttgtgat ccaaattgtg aagaagcttg atgtattcca tgctccggtc
     5461 caggtcgacc aagcggacgt cgagccagtc gcggaggcgg tccaaatcgg ccgcggaatc
     5521 gctgaagcgc cgatcgatct tttccagcgc ccgatccagg cgatcggaga gctcatcctg
     5581 ccggcggctg atccggccga tctccgccca ggaagccagg atttcctcgt gcagcgggag
     5641 ccccaagaga cggatgaccg gggcgaacgg ccggatcgcc cgggagagct tcagcttcac
     5701 gatctctttc aaaccgggtt tggacggcgg cgggacggag ggagccgggg cgggcggctc
     5761 gggagcggcg ggcggggccg ccggcggttc cggcatcgcc tcgagcggct cgacggggcg
     5821 ccgggcggcg gcgcgcttga cctgggccat gatcgaggcg acatcgaaat gatcgtaatc
     5881 gatctcgatt ccctcgatcg tttccttgcc ggcgggcttg acgtcgtcca tcgcgttctc
     5941 ctcgaatccc cccgtcagtc cttcttcgga tacaggtggt ttttatagac cgcctggaca
     6001 tccaggatgt cgggcaaggg acgcaggggc atctcctcga tttcccggac ctcgcggtcg
     6061 gtcaggaggc cttctttctt ccgggcttcg atccgggcct cgatctcccg ctcgattttc
     6121 gcggtttcgg ccttgtcgag gcatttgatg gtcagaaaat cggacattcc tcggctcctc
     6181 gcttatccgt cgggtgcttg cgattgtccc ctcattctat tcaaatccgg aaggagaggc
     6241 aacttgaagg ttctccgccc gttggggcgg aaaaagcctt gggaaacgcc tcgtccggac
     6301 gatgtccgct ccggctttcg gcgtctccgc cggcccccgc ggggaaaaga ggcaaattga
     6361 atctccccgg ccgatgt
//
LOCUS       DAOOXH010000117         6096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_14883_length_6096_cov_8.884953, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000117 DAOOXH010000000
VERSION     DAOOXH010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14883_length_6096_cov_8.884953"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PKV64_10475"
     CDS             <1..450
                     /locus_tag="PKV64_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HPN17538.1"
                     /translation="DFSHAPKYIPDLLAAAAQADLVLGSRFVPGGRDADRGLHRRFIT
                     FCVGTFIRRLYGLSIRDVSSGYRLYRREVLEAVDLDDLISVGQSVVLEILRKTALLGF
                     RIAEVPIVFVDRVRGRTKLDFLSLLETLVMAVKFKKRYTRETVKAAR"
     gene            447..1241
                     /gene="rsmA"
                     /locus_tag="PKV64_10480"
     CDS             447..1241
                     /gene="rsmA"
                     /locus_tag="PKV64_10480"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPN17539.1"
                     /translation="MKTRRQVLGQHFLHDPRLLDKIARTIDPLPEDVVVEIGPGKGAL
                     TRALSPLVRMIVAVEKDRILAESLNAEKPGNVAVVEGDALEVSFERLIRETAGVSPPV
                     KIAGNLPYSISGPFLARVWEERRLFSRASFLLQREVAERIAAGPGTKEYAPLSIMLQI
                     FFEVKILFRVAPGSFSPPPKVDSAVVSMIRRAAPLHPVADEAGFRKFLGGAFAQRRKT
                     LLNNFLAMGIPPDKGEAALASLGLAKTVRPEQVPIPAWISLVSLVR"
     gene            1587..2567
                     /locus_tag="PKV64_10485"
     CDS             1587..2567
                     /locus_tag="PKV64_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPN17540.1"
                     /translation="MKRFDYYRRLWRIYRSYAGKETRLAHMPIRLWIEATSTCGLSCV
                     MCPNKSLPREQRGFMDFGLFKKIIDEAKGFVFDVHLLHRGESLAHPDFFRMIRCAHDA
                     GIVTRFHTNGTLLTEEKSRLLLDSGIDQFAFSFDGFTAEAYERIRAGAKFEKTVGNIV
                     RFLELKKEMKRKKPVTFIELIHFPDVFRKTDRAAKKAFLARFKGLPLDKVHVKELHNW
                     AGETSGGPDRSKPYRPCTFLWHALIIFWDGSVLPCTQDFFGSYPLGNVRDSSLREIWN
                     NDRMVALREKLIARTIDDLPTCSKCDRLWRPTLLGIPREYLGRLLAGKMN"
     gene            complement(2578..3546)
                     /locus_tag="PKV64_10490"
     CDS             complement(2578..3546)
                     /locus_tag="PKV64_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17541.1"
                     /translation="MNHNRSLAFLAAGMIVLAFLGCRGDGSAVVSGGTADGQAAGLSA
                     RRPLADFDGRIVFQSDLGGNNDIYLLTPAGLRRLTDDPASDEFPRWSPDGRFIAFTSN
                     RGGSYQIYVMNDDGTNVRRVTNGPGEAIEQGWHPDGKKIAYTIQRKRAVGRSYRLWTA
                     DLVSGSVTPLLPDFDGSAALPDFSPAEPLLAFTGKRTIGWDAFVADLRSGEVRALSEG
                     GKSCRPRFSPDGSRIAYVSSTADGKGDIWLVNPDSGGRERLTERPETYDYFPAWSPDG
                     RWIVFASGTEHYPTEGVWELALIDPASKRVVPLFKSGGRDVFPDWR"
     gene            complement(3591..5603)
                     /locus_tag="PKV64_10495"
     CDS             complement(3591..5603)
                     /locus_tag="PKV64_10495"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPN17542.1"
                     /translation="MTKRALPVLGLIAASLVFVSPAFAGENISNSPAWPSWAPRATAD
                     AAGNFYVVWTELYPSGGGDLFFTKYTKSAKTWSTPQNLSGSGRVTSEGNDNAGIACDG
                     SGNVHVVWTERTIVRLRTLSGGGNWSTAGTIGEGALLEGAKIAASGAGNLYIVWWGDD
                     GIVRSRSRVNGNWEAARRISSSRRSKFADIAVGQSSVMAVFSQKGSYYYNAAYVLRGT
                     SFGAGWSAPAVVSGENRDQIYPDCEFLNGTAPQVVFIYENAAGEPSNVTHCAWTGNGF
                     GPRQVISYPGATHCPSLIEKSGVLVATWQIGGWNRGLSIYYNYFYNGQWKGPTIIPDS
                     RMATYSDAALDPAGAALAVWDGNGDIMALIMTSGESPTPNNPPVADFSFHPATGSAPL
                     TVNFDAGASTDPDGTIAAYDWIFGDGQTASGRTVVHTFQTPGTFTNSLTVTDNLGATN
                     TKVKNLEVINKAPVPAFTMSSPSLIVTVKIDFDAGSSYDPDGTIAHYDWIFGDGWSAQ
                     GQRFSRVFTRPGTYVLKLTVTDNFGKKASLTKTFTLLALQPPLNVRWESFVDESLFQT
                     RVVTDVQWQANPANEAIAPIVKYRVFRKKPEADAAAYQLAAETDGTTFFWRDTGVTEA
                     GRYVYLVKSVDAAGHESLMPDEAASNALGKTPSRRSQGVFVPTIRR"
     gene            complement(5613..>6096)
                     /locus_tag="PKV64_10500"
     CDS             complement(5613..>6096)
                     /locus_tag="PKV64_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPN17543.1"
                     /translation="KAVGPDGQEVRYDWKFGDGGSGSGRNTSHTFLTAGTYNVTLTIT
                     DNINRTDTVTKQIVVLKTNPLVPQNLSATITLDRVWTNPKITFNLFWAVNPDNIPEHI
                     EAYAIYMKEDDGEYVRLLTLSPSTLSASFEFTDLKKKRSFAISTLGYGGTESTWGYFQ
                     "
BASE COUNT         1037 a         1983 c         1934 g         1142 t
ORIGIN      
        1 gatttctccc acgccccgaa atacatcccg gacctgctgg ccgcggccgc ccaagccgac
       61 ctcgtcctcg ggtcccggtt tgttccgggc ggccgggatg ccgaccgcgg ccttcatcgc
      121 cgcttcatca ccttctgcgt cgggacgttc atccggcggc tttacgggtt gtcgatccgc
      181 gacgtctcct cgggctaccg tctgtaccgg cgcgaggttc tcgaagccgt ggacctggac
      241 gacctgatct ccgtcggcca atccgtcgtc ctggaaatcc tccgcaaaac cgccctcctc
      301 ggcttccgga tcgccgaggt gccgatcgtc ttcgtcgacc gggtccgcgg ccggacgaag
      361 ctggattttc tctcccttct cgaaaccctg gtcatggccg tcaaattcaa gaagcgctac
      421 acccgggaga cggtgaaggc cgcgcgttga aaacccggcg ccaggtcctc ggccagcatt
      481 ttcttcacga tccccggctg ctcgataaaa tcgcccggac gatcgatcct ctccccgagg
      541 acgtggtcgt cgagatcggc cccggcaagg gggcgctcac ccgggcgcta tcccccctcg
      601 tccgaatgat cgtggccgtc gagaaggacc ggatcctggc cgaatccctg aacgcggaga
      661 agccgggaaa cgtcgcggtc gtcgagggcg acgccctgga agtctccttc gagcgcctga
      721 tccgggaaac ggccggagtt tctccgccgg tgaaaatcgc cggcaacctg ccgtactcga
      781 tctcgggtcc gtttttagcc cgcgtctggg aggagcggcg gctgttttcg cgggcttcct
      841 ttcttctcca gcgggaggtc gcggaacgga tcgcggccgg accggggacg aaggagtacg
      901 ccccgctttc gatcatgctc cagatttttt tcgaggtgaa aatcctcttc cgggtcgccc
      961 ccggctcgtt tagtccgccg cccaaggtcg attcggccgt tgtctcgatg atccgccggg
     1021 cggcccccct tcacccggtc gccgacgagg cgggcttccg gaaattcctc ggcggcgctt
     1081 tcgcccagcg ccggaaaacc ttgctcaata atttcctggc catggggatc cccccggata
     1141 agggggaggc cgccttggcc tccctcggcc tggccaagac cgtccgtccc gaacaagtcc
     1201 ccattcccgc ctggatatcg ctggtttccc tcgtccgtta gcgggtgccc cggtctgagc
     1261 cccgccggga agacggcgcg ggcggccggc gtccaatcaa gctcgtccgc gggcctcagg
     1321 cggcctccgt cgcgccgtcc ggtccttgca ttccgggcgt cgcgggagtg tgatcaacgg
     1381 ctctcttccc catcgcccgg gttaattttt gagacgatga cggccggagc gggcatcgtc
     1441 cggatgaggc tttactcgat gccgccgccc cctcttcccg gggagaacat tctcttcctt
     1501 tcctttgact tgcccttcgg cggcgggcga tgggattccg gtcgcggact tccgcccccc
     1561 gggggaatcg gctacaatac gtcctcatga agcgctttga ctactaccgc cgcctctggc
     1621 ggatctaccg gtcgtacgcc gggaaggaaa cccgcctcgc ccacatgccc atccgcctgt
     1681 ggatcgaggc gaccagcacc tgcggcttgt cctgcgtcat gtgcccgaac aaaagcctgc
     1741 cgcgcgagca gcggggattc atggatttcg gcctgttcaa gaaaatcatc gacgaggcga
     1801 agggctttgt cttcgacgtc cacctcctcc atcgcgggga atcgctcgcc catccggatt
     1861 tcttccggat gatccgctgc gcccatgacg ccggcatcgt cacccgattc cacaccaacg
     1921 gaaccctgct gaccgaggag aaatcccgcc tccttctcga ttccgggatc gaccagttcg
     1981 cgttttcctt cgacggattc acagccgagg cctacgagcg gatccgggcg ggagcgaagt
     2041 tcgagaagac cgtcggcaac atcgtccgct tcctcgagct caagaaggag atgaagcgga
     2101 aaaagcccgt cacgttcatc gagctcatcc actttcccga cgttttccgg aaaaccgacc
     2161 gggcggcgaa aaaagccttc ctcgcccggt tcaaaggcct cccgctggac aaagtccatg
     2221 tcaaggagct gcacaattgg gccggggaga cgtccggcgg gccggaccgg tcgaagccct
     2281 accggccgtg caccttcctg tggcacgccc tgatcatctt ctgggacggt tcggtcctgc
     2341 cctgcaccca ggatttcttc ggatcctacc ccctcggcaa cgtccgggat tcgtcgctcc
     2401 gggagatttg gaacaacgac cggatggtgg ccctccggga gaaactcatc gcccgaacga
     2461 tcgacgacct gcccacctgc tcgaagtgcg accgcctctg gcggccgact ctcttgggga
     2521 tcccccgcga gtacctgggc cggctgctcg ccggcaagat gaactgaagg cgcgggctca
     2581 gcgccaatcc gggaagacgt cccgtccgcc gcttttgaac agcggaacga cgcgcttgga
     2641 cgcgggatcg atcaaagcca gctcccagac gccctccgtc gggtagtgct ccgtccccga
     2701 ggcgaagacg atccaccggc cgtcgggcga ccaggccggg aaataatcgt aggtctccgg
     2761 ccgctcggtc aagcgctccc ggcctccgct gtcgggattc accagccaga tatccccctt
     2821 gccgtcggcc gtcgaggaga cataggcgat ccgcgagccg tccggagaga agcgcggccg
     2881 gcaggacttg cctccctcgc tcagggcccg gacttcgccg gagcgcaggt cggccacgaa
     2941 ggcgtcccag ccgatcgtcc gcttcccggt gaaggccagc agcggctcgg ccggagaaaa
     3001 gtcgggcagg gcggccgaac cgtcaaaatc cggaagaagg ggcgtgacgg acccggaaac
     3061 gagatcggcc gtccataacc ggtaggaccg gccgacggcc cgttttcgtt ggattgtata
     3121 ggcgattttt ttcccgtcgg gatgccagcc ctgctcgatc gcctcgccgg ggccgttcgt
     3181 cacccggcgg acattcgtcc cgtcgtcgtt catcacatag atctggtagg atccgccgcg
     3241 gttggaggtg aaggcgatga aacgcccgtc gggggaccag cgggggaact cgtcgctggc
     3301 cggatcgtcc gtcagccgcc gcaggcccgc cggcgtcagg agatagatgt cgttgtttcc
     3361 gcccaggtcg gattgaaaga cgatccggcc gtcgaaatcc gcaagcggac ggcgggcgga
     3421 tagcccggcg gcttgtccgt cggccgtccc gccggagaca acggcggagc cgtcgccgcg
     3481 gcagccgaga aaagccagga cgatcattcc ggccgcaagg aaggctaggc ttcgattatg
     3541 attcatgcgc gtcatgattc ccccgcgatc ccccagcgcg ggtccggcgt tcaacgccgg
     3601 atcgtcggga caaagacgcc ctgggaccgc cggctcggcg tcttgccgag agcattcgag
     3661 gccgcttcat ccggcatcag gctttcgtgc ccggccgcgt ccaccgattt gacaaggtag
     3721 acgtaccggc cggcttccgt caccccggta tcccgccaga aaaaggtcgt cccgtcggtc
     3781 tcggcggcca attgataggc ggcggcgtcc gcctcaggct tcttccggaa gacgcggtac
     3841 ttgacgatgg gagcgatcgc ctcgttggcc gggttggctt gccattgaac gtcggtgaca
     3901 acccgggttt gaaacaggct ttcgtcgacg aaggactccc aacggacgtt cagcgggggt
     3961 tggagagcca ggagggtgaa cgttttcgtc aatgaggcct ttttcccgaa attatcggtg
     4021 accgtcagct tgaggacgta ggttccggga cgggtgaaaa cccgggaaaa cctctgtccc
     4081 tgggcgctcc agccatcgcc gaagatccag tcataatggg cgatcgtccc gtccgggtca
     4141 taggaggatc cggcgtcgaa gtcgatcttg accgtgacga tcagggacgg ggacgacatc
     4201 gtgaaggctg gcaccggcgc cttgttgatg acctcgagat tcttcacctt cgtgttggtc
     4261 gccccgagat tgtccgtgac ggtcagggag ttcgtaaaag ttcccggcgt ttggaacgtg
     4321 tggacgaccg tccggccgga tgccgtctgc ccgtcgccga agatccaatc gtacgcggcg
     4381 atcgtcccgt cgggatcggt ggacgcgccg gcgtcgaaat tcaccgtcag cggcgccgag
     4441 ccggtcgccg gatggaagga gaaatcggcc acgggcgggt tgttgggcgt cggcgattcg
     4501 ccgctcgtca tgatgagggc catgatatcg ccgttgccgt cccagacggc cagggccgct
     4561 ccggccggat caagggcggc gtcgctgtat gtcgccatcc tcgagtcggg gatgattgtc
     4621 gggcctttcc actggccgtt gtaaaagtag ttgtagtaaa tcgacaggcc ccggttccag
     4681 ccgccgatct gccaggtggc gactaaaacg ccggatttct cgatcaagga cgggcagtgc
     4741 gtcgctccgg ggtaggagat cacctgccgg gggccgaacc cgttgcccgt ccaggcgcaa
     4801 tgggtgacgt tggaaggctc tcccgccgcg ttttcgtaga tgaagacgac ttgcggcgcc
     4861 gtcccgttga ggaactcgca gtccggatag atctggtcgc ggttttcgcc cgagacgacg
     4921 gccggggcgg accagccggc gccgaatgac gttccccgga gaacgtaagc cgcgttgtaa
     4981 taataggacc ccttctggga aaacacggcc atgacggagc tttggccgac ggcgatgtcg
     5041 gcgaacttcg accgccggct cgagctgatc cggcgggcgg cttcccagtt cccgttcacc
     5101 cgggaccggg aacggacgat cccgtcgtcg ccccaccaga cgatgtacag gttgcccgcg
     5161 ccggaagcgg cgattttagc tccttccaac aaggcccctt ccccgatcgt cccggccgtg
     5221 ctccagttcc cgccgcccga cagcgtccgg agccggacga tcgtccgctc ggtccagacc
     5281 acgtggacgt tgcccgagcc gtcgcaggcg atcccggcgt tgtcatttcc ctcgctggtc
     5341 acccggcccg agccgctgag attctggggc gtggaccagg tcttcgcgct tttcgtgtac
     5401 ttagtgaaaa agaggtcccc gccgccgctc ggatagagct ccgtccaaac gacgtagaag
     5461 ttgcccgcgg catccgcggt cgcccggggc gcccaggaag gccaggccgg agaattggaa
     5521 atattctccc cggcaaaggc cggggaaacg aaaacaaggg acgcggcgat cagtccaagg
     5581 accgggagcg cgcgtttcgt cattgtcccc gcttattgga aataacccca ggtgctttcc
     5641 gtgcctccat agcccagcgt gctaatggcg aaggaacgct ttttcttcag gtcggtgaac
     5701 tcgaaagaag ccgagagcgt cgacggcgac agggtgagga ggcggacgta ttcgccgtcg
     5761 tcctctttca tataaatcgc gtacgcctcg atgtgctcgg ggatgttgtc aggattgacc
     5821 gcccagaaca ggttgaacgt gatcttcgga ttcgtccaga cccggtccag ggtgatcgtg
     5881 gccgacagat tctggggcac gagggggttg gttttcagga cgacgatctg tttcgtcacg
     5941 gtgtccgtgc ggttgatgtt gtcggtgatg gtcaaggtca cgttgtaggt gccggccgtc
     6001 aggaacgtat ggctggtgtt ccggccggag cccgagccgc cgtcgccgaa cttccaatca
     6061 taacggactt cctggccgtc ggggccgacg gctttg
//
LOCUS       DAOOXH010000118         6064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_14984_length_6064_cov_8.466800, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000118 DAOOXH010000000
VERSION     DAOOXH010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14984_length_6064_cov_8.466800"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PKV64_10505"
     CDS             <1..424
                     /locus_tag="PKV64_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HPN17544.1"
                     /translation="ATKAAVEEGIVPGGGVALLRSQKALDNLKLEGDLKIGTDIIKRA
                     IEEPLRQIANNAGLEGSVVVEKVREAKPDFGFNALSEKYEDMIKAGILDPTKVVRIAL
                     QNAASIAGLMLTTAGLITEMPEEDKKPAYPTPPGGDMY"
     gene            543..3032
                     /locus_tag="PKV64_10510"
     CDS             543..3032
                     /locus_tag="PKV64_10510"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPN17545.1"
                     /translation="MENQASPGRKRSKTGRGGAFRLKIIDRYFLREILSPFALGLLVY
                     SFVLMANQILQYPELFISQGVSFGDSTKLLLYLVPAILAFTIPMAVIMGFLAGLGRMS
                     SDSESTALKVLGVSHARMTRPLFLFALCGWAATSVLTLYVTPKFNYLWLQTTVKAALD
                     KAQIRVNPREFNATIPGLALYLDDIERGGDWRKAFISFNAGPLESRVVLADRGRLNYY
                     PDSKRATVELFGAVQHSSLGDKPEYYERVDSAHMEQEIDFSGLANAFSSEKRAREQNI
                     LELRAGLAAGRAELDDLLKEKASLAGRGLPPRNPLVLKNEIAVRHKNSNIRSLRVESH
                     KRFALPFVCLIFVFLGLPLGISTRKGGRTSGFTISLVIILAYYVFLTFGERLALDGRV
                     EPWLGVWGGNIVFGAASLILFIRSARERPLLIKWVQRPLPALMAGAPAPAAAREEKES
                     SRLRGLIRGLLLRLPFPNILDRYFFRRYLWIGGLILVSVVSIFCIVTFFDRLDNVYSH
                     EKPVLMLVNYISHRLPEFFQLGLPMTTLMATLLTLGLFHKFNEITAMKACGVSVFRMF
                     IPAFVLAAAIGGLSFFIQENVLPGESQKAAKVWNEINDVSPPSAETQGFAWKANRARD
                     RFFKYDYVNPADMTFRKVWIFEIDHARWTLKRRMFAAGARLIDKTLVLENGWLREFPG
                     AGGSRPPDPVFFDAEAIDVPEGRALFLSVSKDPSAMKFAELGRYIEEVEQTGFNTTRL
                     KVDRASKISFPFVAVIMTLLGIPFAFAMGKRGALVGIGVSLGIALVFWTTIGIFKSLG
                     GVGVLPVFLAAWGPALMFGLLGLYLMLRLKT"
     gene            complement(3081..3566)
                     /locus_tag="PKV64_10515"
     CDS             complement(3081..3566)
                     /locus_tag="PKV64_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPN17546.1"
                     /translation="MAIKMRIKGLVIDPISKMPIVVLQDPTSERILPIWIGVFEANAI
                     ALKIENIATPRPMTHDLMTNLLERLSVAVERVTISDIRNNTFYAVIRCRRGGEDLVID
                     SRPSDAIALALRTDAPIYVEEDVVLRAQSLTLDPSLENPENLRRWLETLPPEDFGKYR
                     M"
     gene            complement(3634..4893)
                     /locus_tag="PKV64_10520"
     CDS             complement(3634..4893)
                     /locus_tag="PKV64_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HPN17547.1"
                     /translation="MRFLADLHVHSPFSLATSGDMTFDTLAAWAKIKGLALVGTGDFT
                     HPEWSRRIRRELEPAGNGFFRLKKAKPPAGGLPDGFRPAPGDVHFILSAELSFIYSQG
                     GRVRKIHLVVLAPGLAAVERLNRRLGRIGNIRSNGRPILGLDAASFCRIVADVDPGCL
                     VIPAHVWTPWFSLFGSRSGFDSVEECFGDMTPFIAALETGLSADPRMIRRVSALDRFT
                     AVSNSDAHSPAKLGREANAFETEFSYAGLAEAIRGGDPRRFLYTVELFPEEGKYYADG
                     HRKCGVVLRPEESPKRMNPCPVCGKILTPGVGRRVGELADRPERPKAAASLVPALHLV
                     PLEEIIAEAAGFRPGGRPGRALYFSLIREFGNEHRILTEVPPAEIGRAAPGRLAAGIE
                     RVRRSRVTVSPGYDGTYGRIRIFDRSL"
     gene            complement(4980..>6064)
                     /locus_tag="PKV64_10525"
     CDS             complement(4980..>6064)
                     /locus_tag="PKV64_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17548.1"
                     /translation="LGYAPLPGTPKLQAEGHVYVRLYLIENGRAGKIVNENDMPAAFE
                     VDPDGYDPEASDWYAFGYPLLAGDYLAATALVSADGARVGIQYSRFSIPDPAGFTDRL
                     DTSTILVMKNFERVESPEPPPVIHRGLLAWSVARITPCLSKTIKVGQPLDFFFYVFGV
                     RPDDAGKCNLQVDFQVDKLTRESDIDGAKTPLFLKDWSWERTPEGGSIEAKGELKNMT
                     GEALSGVEAVVNFFDRENKYISHAGCRIEPEPLSPAGTASFRLVQKDDPLIENATVDF
                     KNAAGDVIAKASLIETALRFDTQTFQSPLISLPLPLKQTFEVRTGDKVEYKKEDLSRG
                     RYVLIIRMLDKMSGLKGEEKIEFTVE"
BASE COUNT         1087 a         1896 c         1902 g         1179 t
ORIGIN      
        1 cgccaccaag gcggccgtcg aggaaggcat cgttcccggc ggcggcgtgg ccctgctccg
       61 gtcccagaag gctctcgaca atctcaagct ggaaggcgat ctcaagatcg gcacggacat
      121 catcaagcgg gccatcgagg agcccctccg ccagatcgcg aacaacgccg ggctcgaggg
      181 ctcggtcgtc gtcgagaagg tccgcgaggc caagcccgac ttcggcttca acgccctgtc
      241 cgagaagtac gaggatatga tcaaggcggg catcctcgat ccgacgaagg tcgtccggat
      301 cgccctgcag aacgcggcct cgatcgccgg cctgatgctg acgaccgccg gcctgatcac
      361 cgagatgccg gaagaggaca agaaacccgc gtatccgact cctcccggcg gcgacatgta
      421 ctaaggcttt ttcaggggag cggggcctcc gcccctttcc cggaaaacga cccggggagc
      481 ggccgaacgg ccgctcccct tttttttacg ccccgcccgc tctttctgct atgataaggc
      541 ccgtggaaaa tcaggcgtcc cccggccgga agcggtccaa gaccggacga gggggggcat
      601 tccgcctgaa aatcatcgat cgttatttcc tccgggaaat cctgtctccc ttcgccctcg
      661 gactcctcgt ctacagcttc gtcctgatgg ccaaccagat tctccagtat cccgagctgt
      721 tcatctccca gggagtctct ttcggcgatt cgaccaagct cctcctgtat ctcgttccgg
      781 ccatcctggc cttcaccatt ccgatggccg tcatcatggg ctttttggcc gggctcggcc
      841 ggatgtcctc ggattcggaa tccacggccc tgaaagtcct gggcgtcagc catgcccgga
      901 tgacccggcc gctgtttttg ttcgccctgt gcggctgggc ggccacatcc gtcctgaccc
      961 tctatgtgac cccgaagttc aattacctct ggctgcagac gaccgtcaag gccgccctcg
     1021 acaaggccca gatccgggtc aatccccggg aattcaacgc caccatcccc gggctggccc
     1081 tctacctgga tgacatcgaa cgcggagggg actggagaaa agcgttcatt tccttcaacg
     1141 ccggcccgct ggaatcccgg gtcgtcctgg cggaccgcgg ccggctgaat tattatccgg
     1201 actccaaacg ggcgaccgtc gaactgttcg gcgccgtcca gcactcgagc ctcggcgaca
     1261 agccggagta ctatgagcgg gtcgattcgg cccacatgga gcaggaaatc gacttttcgg
     1321 ggctggccaa cgccttcagc tccgagaagc gggcccggga gcagaatatc ctcgagctgc
     1381 gagccggcct tgccgccggc cgggcggagc tggacgatct cctgaaggag aaggcttccc
     1441 tggccggccg cggccttccg ccgagaaatc ccctcgtcct caagaatgag atcgccgtcc
     1501 ggcacaagaa ttccaacatc cggtccctgc gggtggaaag ccacaaacgc ttcgccctcc
     1561 cgttcgtctg cttgattttc gtctttctcg gcctgcccct gggaatctcg acgaggaaag
     1621 gcggccggac gagcggcttc acgatcagcc tagtgatcat tctggcatat tacgtcttcc
     1681 tcacgttcgg agaacgcttg gccctcgacg gccgcgtaga accatggctg ggggtatggg
     1741 gaggaaacat cgtcttcggc gcggccagcc tgatcctttt tattcgctca gcccgcgagc
     1801 ggccgcttct catcaaatgg gttcagcgcc cgctgccggc ccttatggcc ggcgcccccg
     1861 ctccggcggc tgcgcgcgag gagaaggagt cctcccgcct ccgcgggctg atccgggggc
     1921 tgctcctccg ccttcctttc ccgaacatcc tggaccgcta tttcttccgc cggtatctct
     1981 ggatcggcgg cctgatcctc gtcagtgtcg tctcgatctt ctgcatcgtg acgtttttcg
     2041 accgtttgga caatgtctat tcccacgaga agccggtcct gatgctggtg aattacattt
     2101 cccaccgcct cccggaattc ttccagctgg gcctgccgat gaccaccctg atggccaccc
     2161 ttctcaccct tggccttttc cacaagttca acgagatcac ggcgatgaag gcctgcggcg
     2221 tgagcgtctt ccggatgttt atcccggctt tcgtcctggc ggcggccatc ggcggcctgt
     2281 cgttcttcat ccaggaaaac gtcctgccgg gggaaagcca gaaagcggcc aaagtctgga
     2341 acgaaatcaa cgatgtctct ccgccgtcgg ccgaaacgca gggtttcgcc tggaaggcca
     2401 accgggcccg ggaccgtttt ttcaaatacg attacgtgaa tccggccgac atgaccttcc
     2461 gaaaggtctg gattttcgag atcgaccacg cccgctggac cctgaaaagg cggatgttcg
     2521 cggccggggc tcgcctgatc gacaagaccc tggtcctgga gaacggctgg ctccgggaat
     2581 ttcccggtgc cggcggctcc cgtccgcccg atccggtctt tttcgacgcc gaggccatcg
     2641 acgtccccga gggccgggcc ctctttttaa gcgtctccaa ggatccgtcg gcgatgaagt
     2701 tcgcggagct cggccgttat atcgaggaag tggagcagac gggcttcaac accactcgtc
     2761 tgaaggtgga ccgggcttcg aagatctcgt ttcccttcgt cgccgtcatc atgaccctgc
     2821 tcggcatccc gttcgccttc gccatgggca agcgcggcgc ccttgtcggg atcggcgtca
     2881 gcctggggat cgccctcgtt ttctggacga ccatcggcat cttcaagagt ctggggggcg
     2941 tgggagtcct gcccgtcttt ttagccgcct gggggccggc cttgatgttc gggctcttgg
     3001 ggctttattt gatgcttcga ctgaaaactt gacggcttcc gccggccggc gggagggaaa
     3061 aggcgggctc gaccggcccc ttacatccgg tattttccga aatcctcggg gggaagcgtc
     3121 tcgagccacc ggcgcaggtt ctccgggttt tcgagcgacg gatccagggt gaggctctgg
     3181 gcccggagga cgacgtcttc ctcgacgtaa atcggggcgt cggtcctgag agccaaggcg
     3241 atcgcgtccg aaggccggga gtcgatgacc aggtcctcgc cgccgcggcg gcaccggatg
     3301 accgcgtaga atgtgttgtt ccggatgtcg ctgatcgtca cccgttcgac ggccacggac
     3361 agccgttcga gaaggttggt catcaggtcg tgggtcatgg gccggggcgt ggcgatgttc
     3421 tcgattttaa gagcgatggc gttggcttcg aagaccccga tccagatggg aaggatgcgc
     3481 tcgctcgtcg ggtcctggag gacgacgatg ggcatcttgg atatggggtc gatgaccaga
     3541 cccttgatcc gcattttgat cgccaacgat gactcctaga ttaaaaatat aaaatccggg
     3601 agctcggaag tcaaggaacg gcgccgattt ttctcaaagg gagcggtcga aaatccttat
     3661 tcttccgtac gttccgtcgt accccggcga aaccgtcacc cggctccggc ggacccgctc
     3721 gattccggcg gcgagacgac cgggcgcggc ccgcccgatc tcggccggag ggacttccgt
     3781 cagaattcga tgctcgtttc caaactcccg gatcagggag aagtaaagcg cccggcccgg
     3841 acgcccgccc ggcctaaagc ccgcggcttc ggcgatgatc tcctccaagg ggaccaagtg
     3901 aagcgccggg accaggctcg ccgccgcctt cggccgctcc gggcggtcgg ccagctcgcc
     3961 gacccggcgg ccgacgccgg gcgtcaggat tttgccgcag acggggcagg gattcatccg
     4021 cttcggcgat tcctccggac ggaggacgac cccgcatttc cggtgaccgt cggcgtaata
     4081 cttcccttcc tccgggaaaa gctcgacggt atagaggaac cggcgcgggt cgccgccgcg
     4141 aatcgcctcc gccaggcccg cgtaactgaa ttccgtctca aaggcgttgg cttcgcggcc
     4201 gagcttggcc ggggaatggg cgtcggaatt ggatacggcc gtgaaccggt ccagggcgga
     4261 gacgcggcgg atcatccgcg gatcggccga aaggcccgtc tcgagggcgg cgatgaacgg
     4321 ggtcatgtcg ccgaagcatt cctcgacgga atcgaagccc gatcgggagc cgaaaagcga
     4381 aaaccagggc gtccagacgt gggcgggaat gacgagacat ccgggatcga cgtcggctac
     4441 gatccggcag aaggaagcgg catcaagccc gaggatgggc cggccgttgg accggatatt
     4501 cccgatccgg cccaggcgcc ggttgaggcg ttcaaccgcc gcgagaccgg gggcgagcac
     4561 aacgagatgg atttttcgga cccggccgcc ctgggaataa atgaagctca attccgcgga
     4621 gaggatgaag tggacgtcgc cgggtgccgg cctgaagccg tcgggaaggc cgccggccgg
     4681 cggctttgct tttttcagcc ggaaaaagcc gtttccggcg ggctcgagct cccgccggat
     4741 ccggcggctc cattccgggt gggtgaagtc ccccgtccca acgagggcga ggcccttgat
     4801 tttcgcccag gcggccaggg tgtcgaaggt catgtccccg gaggtggcca gggaaaacgg
     4861 tgaatgaacg tgcaggtcgg cgaggaaacg catgccgccg tccagtgtaa aaaacaagcc
     4921 ggccgatggc aagggcggcc ggcccggatt tttttcgccc cgaatcgcgc cggaacggat
     4981 tattcgaccg tgaactcgat tttttcctcg cctttgaggc cggacatctt gtcgagcatc
     5041 cgaatgatca ggacatagcg accgcgggag agatcctcct tcttgtattc caccttgtcg
     5101 cccgtccgga cttcaaacgt ttgtttgagg ggaagaggca ggctgatcag cggcgattgg
     5161 aacgtttggg tgtcgaagcg gagggccgtt tcgatgagcg aagccttggc gatgacgtcg
     5221 ccggccgcat ttttgaaatc gaccgtcgca ttttcgatga gcggatcgtc tttctgaacg
     5281 agacggaatg aggccgtccc ggccgggctg agcggttcgg gctcgatccg gcagccggcg
     5341 tggctgatgt atttattttc ccggtcgaag aaattgacga cggcttcgac gccggacagg
     5401 gcttccccgg tcatgttctt caattcgccc ttcgcttcga tcgagccgcc ctcgggcgtt
     5461 ctctcccaac tccagtcctt taagaaaagc ggcgttttag ccccgtcgat atcggattcg
     5521 cgggtcagtt tgtccacttg aaagtcgact tggagattgc acttaccggc gtcgtcggga
     5581 cgcaccccga aaacataaaa gaagaaatcg agcggttgac cgaccttgat ggtcttcgac
     5641 aggcaggggg tgatccgggc gacggaccaa gctaaaagcc cccggtgaat gacgggcggc
     5701 ggctcgggcg actcaacccg ctcgaagttt ttcatcacga gaatggtgga tgtatccagc
     5761 cggtcggtga atccggccgg atcggggatc gaaaatcggc tatactggat gcctacccgg
     5821 gcgccatcgg ccgagacaag ggccgtcgcg gccaggtaat cccccgccag aagcggatag
     5881 ccgaacgcgt accagtcgga ggcctcggga tcgtatccgt ccgggtcgac ttcgaaagcc
     5941 gccggcatgt cgttttcgtt gacgattttg ccggcccggc cgttttcgat gaggtagagc
     6001 cggacataga catggccctc ggcctggagc ttgggagttc ccgggagcgg ggcgtatccc
     6061 agat
//
LOCUS       DAOOXH010000119         6058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_15007_length_6058_cov_8.725304, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000119 DAOOXH010000000
VERSION     DAOOXH010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15007_length_6058_cov_8.725304"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..162
                     /locus_tag="PKV64_10530"
     CDS             <1..162
                     /locus_tag="PKV64_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPN17549.1"
                     /translation="FALVVGNPARIAGWMSEAGEKLRFDAAGFAACPRSGRRYRLENG
                     RVRDVAGEE"
     gene            167..1306
                     /gene="wecB"
                     /locus_tag="PKV64_10535"
     CDS             167..1306
                     /gene="wecB"
                     /locus_tag="PKV64_10535"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HPN17550.1"
                     /translation="MKIASIVGARPQFVKAAPVSRAIREAAAAGRDIAEILVHTGQHY
                     DGNMSDIFFEELGISAPDYNLGVGSASHGVQTGAMLAAIERVLEKERPDWVLIYGDTN
                     STLAGALAAAKMRLPIAHVEAGLRSYDRRMPEEINRIVADQLSTALFCPSRNAAANLR
                     KEGIRDGVSIVGDVMAEALEYAVVRSMREAEEKPGSGEYGYVLATVHRAENTDDPARL
                     RNILKALEAIGRIRPVVFPVHPRTKKMIAAAGWKPAAKGPEKSGARRAKTTAGAGIRF
                     IDPVGYLEMARLESGAEMILTDSGGVQKEAYWLKVPCITLRDRTEWVETVASGWNVLA
                     GTDTKKIIRAAENFTIPEKSAPLYGRGPKSAAPASRRIVDILLKS"
     gene            1353..1583
                     /locus_tag="PKV64_10540"
     CDS             1353..1583
                     /locus_tag="PKV64_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HPN17551.1"
                     /translation="MKPIKYRRLAEAASLGLSLPSGIAVGMFFGYWLDKWLKTEPWMI
                     ILWTLLGIASGLISLVQGIKKFLREGDSEKED"
     gene            1625..2086
                     /locus_tag="PKV64_10545"
     CDS             1625..2086
                     /locus_tag="PKV64_10545"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HPN17552.1"
                     /translation="MSLERSDPAPAPPSSRSAGPPDGSPARSINEAAVRRIPLEILVL
                     TAVGAVVSAAVFNATAGGLFLAGGALSTLSFLWMKRTVAGILNRKKAAAVRSGLFFYA
                     LRFVLILGIFFLIILAYPDKILAFAAGFSMAVPAFVVEAFRAYALMKTWKD"
     gene            2074..2865
                     /gene="atpB"
                     /locus_tag="PKV64_10550"
     CDS             2074..2865
                     /gene="atpB"
                     /locus_tag="PKV64_10550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPN17553.1"
                     /translation="MEGLEHPLGIVVLVNRILGRPVAAILGLLGIKVADPARCIPDYI
                     VMVFIVAAAMIVFFTLAGKKRDLVPGRFQVVTESIIRFFEGQIVEIVGPEGRKYTPVI
                     GTVGLFILCGNLLGLIPGLMSPTAKLNVTIGCALFVFVYYHAQGIKTQGFFKYLKHFM
                     GPIPAIAPLMIPIELISHFSRVVSLSIRLFSNIFAEEVLIVVIASIIPVFLPLPFMAL
                     SIFTAFLQAFVFVLLTCIYLSGAVEQEEEHASSAPSEARLKEVHA"
     gene            3077..3421
                     /locus_tag="PKV64_10555"
     CDS             3077..3421
                     /locus_tag="PKV64_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPN17554.1"
                     /translation="MSKTLKTLGLTIFFAVLAWMPLMAQEGGAPDTKSKLYMLSLIVA
                     GAVITLAAAAGAFCQAKAISSACEGISRNPAGAPTIRFLLLLGLILIETLVIYALLVA
                     FIILMVKWGNYA"
     gene            3500..4390
                     /locus_tag="PKV64_10560"
     CDS             3500..4390
                     /locus_tag="PKV64_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPN17555.1"
                     /translation="MAAPFFRILGETLRRFDRDRCFQYSIIISYFALMCAVPLLALFA
                     WITARIMGNSEIAVRSLNLFTEDFFARFSPSFFEKMSSVADSFNQLGWVGIIGSLIAA
                     SLLFSNLIRIINEIFRTNYQRSFVYNRMMEYLIMFVTGVILFVSLSITAVWTAVHGAI
                     ESSDFVVTYLNPGVVTFFDNVFIQYLIPLALSILVLFVIYKFIPERKVHTRAALLAAV
                     IAAVLWEVFKRLFVVYVVRFSAIGIVLSKLVQGTLTSIIFFLLWLSFSLAIMLWGAEL
                     AAVVNERLDARPPAAGQAAA"
     gene            4539..5582
                     /gene="lpxD"
                     /locus_tag="PKV64_10565"
     CDS             4539..5582
                     /gene="lpxD"
                     /locus_tag="PKV64_10565"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPN17556.1"
                     /translation="MKITVGKLAEFLGLVFEGDGSIEITGVSTMDKAGPADLVFFTQA
                     RLRPLLEGTRASAGILPAGEPLDRVPLKAVLRAEQPHLAFLRAIGLIIPPRSPAPGVH
                     PGAFVSPDARLAEGVSVGAMSVVGAGAEIGPGTIIHPLVSIYEGVRVGAGCILHSGVS
                     LREDVRLGDRVILHNGVQIGGDGFGYLPGPDGRHLKIPQVGTVIIEDDVEIGANSTVD
                     RAALGATIVRRGTKIDNLVTVAHNVEVGEDAILVAQVGIGGSSKIGRRAILSGQVGIP
                     DHIDIGEGVIVAAKTGVTKNVPAGTMVAGIPHLDIRDWRKLWAVAPQLYDLVKEFKKM
                     KARVAELETALKK"
     gene            complement(5601..>6058)
                     /locus_tag="PKV64_10570"
     CDS             complement(5601..>6058)
                     /locus_tag="PKV64_10570"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPN17557.1"
                     /translation="LMEAVYLAKYASRVHLIHRRDRLRGTKILQERLFADPKITVEWS
                     AVIDQILGRDRVEGLVLRDTNDGSVRELAVEGLFVSVGMDPQSEFVRELLDLNEWKEI
                     RVDGRLATSRPGIFAAGDVIDAAPHQVATAAGTGVAAALSVVEYLSKTG"
BASE COUNT         1038 a         2021 c         1780 g         1219 t
ORIGIN      
        1 ttcgccctcg tcgtcggcaa tccggcccgg atcgccggct ggatgagcga ggccggagag
       61 aagctgcgct tcgacgccgc gggattcgcc gcctgtcccc gctccggacg tcggtaccgc
      121 ctcgaaaacg ggcgcgtccg cgacgtcgcc ggcgaggaat gagccgatga aaatcgcttc
      181 gatcgtcgga gcccgtcccc agttcgtcaa ggccgcgccc gtcagccggg cgatccgaga
      241 agcggccgcc gccggccggg acatcgcgga aatcctcgtt cacaccggcc agcattacga
      301 cggcaacatg tcggacatct tcttcgagga gctgggcatc tccgcccccg attacaatct
      361 gggcgtcgga tcggcttcgc acggcgtcca gacgggggcg atgctcgccg ctatcgaacg
      421 ggtcctggag aaggagcggc cggattgggt tctcatttat ggcgacacga attccaccct
      481 ggccggggcg ctggccgcgg ccaaaatgcg ccttccgatc gcccacgtcg aagccggcct
      541 ccgctcttac gaccgccgga tgcccgagga aatcaaccgg atcgtcgccg atcaactgtc
      601 gacggcgttg ttctgcccct caagaaacgc agccgccaac ctccgcaagg aaggcatccg
      661 tgacggcgtg tccattgtgg gggacgtcat ggcggaagca ttggaatacg cggtcgtccg
      721 atccatgcgg gaggcggagg agaagcccgg aagcggggaa tacggatacg ttctggcgac
      781 cgtccaccgg gcggaaaata cggacgaccc ggcccggctt agaaatattc ttaaagccct
      841 cgaggcgatt ggccgcatcc ggcccgtcgt gtttcctgtc catccgcgga caaagaagat
      901 gatcgcggcc gccggttgga agccggcggc aaaaggcccc gaaaaaagcg gggcgcgaag
      961 ggcgaagacg acggccggcg cggggatccg atttatcgac cctgtcggtt atctcgaaat
     1021 ggcccggctg gaaagcggag cggagatgat cttgaccgac tcgggcggcg tgcagaagga
     1081 agcctactgg ctcaaggtcc cgtgcatcac tctccgggac cggacggaat gggtggagac
     1141 ggtcgcctcg ggctggaacg tcttggccgg gacggatacg aaaaagatca ttcgggcggc
     1201 ggagaatttc acgattcccg aaaaatcggc cccgctttac ggccggggcc cgaaatcggc
     1261 ggcccccgcc tcgcggcgca tcgtcgacat cctcttgaaa tcatgagccg gggccggagc
     1321 acaccgaggg ccggttctcc ggcgggaagg cgatgaagcc gatcaaatac cgccggctcg
     1381 ctgaagccgc ctccctgggc ttgtcgcttc cgtcggggat cgccgtcggg atgttcttcg
     1441 gttattggct ggataaatgg ctgaaaacgg aaccttggat gatcatcctc tggacgttgc
     1501 tgggcatcgc ctccggactg atcagcctcg tccagggaat caagaagttc ctgcgggagg
     1561 gcgactcgga aaaggaagac taggccctct ccctgtgcta gaataagcgg cgaggccgaa
     1621 tccgatgagc cttgaacgat ccgatcccgc tccggctccg ccgtcctctc gatcggccgg
     1681 ccctccggac ggttccccgg cccggtcgat caatgaagcg gcggtccgcc gcattcccct
     1741 cgaaatcctg gttctcacgg ccgtcggcgc cgtcgtttcg gcggcggtct tcaacgcgac
     1801 ggcgggaggt ctcttcctgg ccggcggcgc cctgtcgacc ctgtcttttc tctggatgaa
     1861 aagaaccgtc gccggaattc tgaaccgaaa aaaagcggcc gccgtccgct ccggcttgtt
     1921 tttctacgcc ctccgcttcg ttttgatttt ggggattttt ttccttataa tcctcgctta
     1981 cccggataaa attctggcct tcgccgcggg attctcgatg gccgtcccgg cttttgtcgt
     2041 cgaggctttc cgggcgtacg ccctcatgaa gacatggaag gactagaaca ccccctcgga
     2101 atcgtcgtcc tggtcaaccg gatcctcgga cgtccggtgg ccgcgatcct cggcttgctc
     2161 gggatcaaag tcgccgatcc cgcccgctgc atccccgatt acatcgtcat ggttttcatc
     2221 gtcgccgcgg cgatgatcgt tttcttcacc ttggccggga agaagcgcga tctcgtcccc
     2281 ggccgcttcc aggtcgtcac cgaatcgatc atccgattct tcgaggggca gatcgtcgag
     2341 atcgtcggcc cggaggggcg gaaatacact cccgttatcg ggacagtcgg gctgttcatc
     2401 ctctgcggca acctcctggg gctgattccg ggcctcatgt cgcccacggc caagctcaac
     2461 gtcaccatcg gctgcgccct gttcgtgttc gtttactacc acgcccaggg gatcaagacc
     2521 cagggattct tcaaatatct caagcatttc atgggcccca tcccggccat cgccccgctg
     2581 atgatcccga tcgagttgat cagccatttt tcccgggtcg tctccctgtc catccggttg
     2641 ttcagcaaca tcttcgccga ggaagtcctg atcgtcgtca tcgcctcgat catcccggtt
     2701 tttcttcccc ttcccttcat ggccctttcc atcttcaccg cgtttcttca agccttcgtc
     2761 ttcgtccttc tgacctgcat ctatctgtcc ggcgcggtcg agcaggagga ggagcacgct
     2821 tcctccgccc cgtccgaagc ccgcctcaag gaagttcacg cgtgaatccg cccgcccgaa
     2881 tgagccctcc ccgttccaag attctccttg ttccgcgcct cgccgaagag gcctcgttct
     2941 ctccagagga acggattcat caacgcatca ccccccggcg cgcgtccccg agcgccggga
     3001 tcttaacctc gttttcattt cttctcttag gcgaggggtc tcgactcgcg tataatggag
     3061 agtaaggagt tccgtcatgt cgaaaacctt gaaaacgctc ggtctcacga tctttttcgc
     3121 cgtcctcgct tggatgcccc tcatggccca ggaaggcgga gcgcccgaca cgaaatcaaa
     3181 gctctatatg ctgtcgctga tcgtcgccgg cgcggtcatc accttggccg cggcggccgg
     3241 agcgttctgc caagccaaag ccatcagcag cgcctgcgag ggcatctccc gcaacccggc
     3301 gggcgccccg acgatccgtt tccttctcct tctggggctg attctgatcg aaacgctcgt
     3361 catctacgcc ttgctcgtgg ctttcatcat cctgatggtc aagtggggca actacgcctg
     3421 atttgaagcc ttgcttggga aaccctccga ccggcggttt cttcccgccg gccggagggt
     3481 cccgtgataa ggccgagtga tggccgcccc gtttttccgg attctcggcg aaaccctccg
     3541 gcggttcgac cgcgaccgct gtttccaata ctccatcatc atctcctatt tcgccctgat
     3601 gtgcgccgtg ccgcttttgg ccctcttcgc ctggatcacg gcccggatca tgggcaactc
     3661 ggaaatcgcc gtccgcagcc tgaatctctt caccgaggat tttttcgccc ggttcagccc
     3721 ctccttcttc gagaaaatgt cctcggtcgc cgacagcttc aatcagctcg gctgggtcgg
     3781 catcatcggc tccctgatcg ccgccagcct gctgttctcc aacctgatcc ggatcatcaa
     3841 cgagattttc cggacgaact accagcgctc gttcgtctac aaccggatga tggaatacct
     3901 gatcatgttc gtgaccggcg tcatcctgtt tgtctcgctt tcgatcacgg ccgtctggac
     3961 ggccgtgcac ggggccatcg aatcaagcga cttcgtcgtc acctatctca acccgggcgt
     4021 cgtcaccttc ttcgacaacg ttttcatcca gtacttgatt cccctcgccc tgagcatcct
     4081 cgtcctgttc gtgatctaca agttcatccc cgagcgaaaa gtccataccc gggcggccct
     4141 cctggcggcc gtcatcgccg ccgttctctg ggaagtcttc aagcggcttt tcgtcgtcta
     4201 cgtcgtccgt ttttcggcca tcggcatcgt cctcagcaag ctcgtccagg gaacgctgac
     4261 ttcgatcatc tttttccttc tctggctttc gttttccctg gccatcatgc tctggggcgc
     4321 cgagctggcc gccgtcgtca acgagcgcct ggacgcccgc ccccccgcgg ctggccaggc
     4381 cgccgcctga atttaaaagg gacatccccc ctttttcggc ctttccttgc ggcccgtggc
     4441 cgcggcctcc tccacgcgtc tgtcgctcga gtgtaatacg catgtcatac aggtataata
     4501 catgcttgac taggcatcat aataaggcgg aggccgggat gaaaatcacc gttggcaagc
     4561 tggcggagtt tctaggattg gtttttgagg gggacggatc catcgagatc acgggcgttt
     4621 ccaccatgga taaggccggt ccggccgact tggttttctt cacccaagcc cggctgcgcc
     4681 cgctcctgga gggcacccgg gcctcggccg gcattctccc ggccggcgag ccgctcgatc
     4741 gcgtccccct caaggccgtt ctccgggccg aacagccgca tctggccttc ctccgggcga
     4801 tcggcctgat catccccccg cgctcccccg ctccgggcgt ccatcccggc gccttcgttt
     4861 ctccggacgc ccggctggcc gaaggcgtct cggtcggagc gatgtcggtc gtcggcgccg
     4921 gggcggagat cggtccgggc acgatcatcc atcccctcgt ttcaatctac gagggcgtcc
     4981 gggtcggagc cggctgcatc cttcattcgg gcgtgtcgct ccgcgaggat gtccgcctcg
     5041 gcgaccgggt catcctccac aacggcgtcc agatcggcgg agacggcttc ggctacctcc
     5101 ccggcccgga cggacggcac ctcaagatcc cccaggtcgg aacagtgatc atcgaggacg
     5161 acgtcgagat cggggccaat tcgacggtgg acagggccgc gctcggggcc acgatcgtcc
     5221 gccgcgggac gaaaatcgac aatctcgtca ccgtcgccca caacgtcgag gtcggcgagg
     5281 acgcgatcct cgtggcccag gtcgggatcg gcggaagctc gaagatcgga cgccgggcga
     5341 tcctcagcgg acaagtcggg atcccggacc acatcgacat cggcgaaggc gtcatcgtcg
     5401 cggccaagac aggggtcacg aaaaacgtcc cggccgggac catggtcgcc ggcatccccc
     5461 atctcgatat ccgcgactgg cggaagctgt gggccgtcgc ccctcagctc tacgacctcg
     5521 tcaaggaatt caaaaaaatg aaagcccggg tcgccgaact cgagaccgcc ctcaagaaat
     5581 gacccggccc cggaggcccg tcacccggtc tttgaaagat attcgacgac gctgagggcg
     5641 gcggcgacgc cggttccggc ggccgtggcc acctgatgcg gagcggcatc gatgacatcg
     5701 ccggccgcga agatcccggg acgggacgtc gccaagcggc cgtcgacccg gatttccttc
     5761 cattcgttga ggtcgagaag ttcccgaacg aattcgctct gaggatccat gccgaccgag
     5821 acgaacaggc cttccacggc cagctcgcgg accgacccgt cgttcgtgtc ccggaggacg
     5881 agcccctcga cccggtcgcg gccgaggatc tgatcgatga cggcgctcca ttccaccgtg
     5941 atcttgggat cggcaaagag gcgctcctgg aggattttcg tcccgcggag gcggtcccgc
     6001 cgatggatga ggtggacccg ggaggcgtac ttcgccaagt agaccgcttc catgagag
//
LOCUS       DAOOXH010000120         5981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_15296_length_5981_cov_6.964900, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000120 DAOOXH010000000
VERSION     DAOOXH010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15296_length_5981_cov_6.964900"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PKV64_10575"
     CDS             complement(<1..922)
                     /locus_tag="PKV64_10575"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPN17558.1"
                     /translation="MLDEDIREGLTFDDVLILPAKSDVEPADVSTITRFSRRIELRIP
                     LVGAAMDTVTRSTMAIALAQQGGLGIIHRNMPAEVQADEVDKVKRYESGMIHEPISMR
                     PTDTIGQARDTMRHFHISGLPITDAANKLLGILTNRDIRFETRSDITIDRVMTRSLVT
                     VPVGTSLAEAEKLFHQHKIEKILIVDESYHLKGMITYKDILKRIQYPNAAKDALGRLR
                     VGAAVGVGDEAIGRVQALIAAKADVLVVDTAHGHSRRVLDMVKLIRREFPDQELVAGN
                     IGTAEAARELIDLGVDAVKVGVGPGSICTTR"
     gene            complement(937..3528)
                     /gene="secA"
                     /locus_tag="PKV64_10580"
     CDS             complement(937..3528)
                     /gene="secA"
                     /locus_tag="PKV64_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPN17559.1"
                     /translation="MVKWLSEKLFGTKSERDLKKLTPYVGLINDFESRIRELSDDALR
                     AKTGEFREKLAQGAALDDLLPEAFAVVREAARRTTGMRPFDVQLIGGVVLHQGKIAEM
                     KTGEGKTLVSTLPAYLNALEGRGVHIVTVNDYLAKRDTEWMGPIHRFLGLTVGTIQHD
                     MPDADRLAAYRADITYGTNNEFGFDYLRDNMKFRLSDLVQRPFNYAVVDEVDSILIDE
                     ARTPLIISGPSEGSTDLYQKADTVVRRFAADTHFKIDEKSRAVSLLEEGVAAAEKALH
                     VSNLYDLSQMDLVHGLNQALKAHFLFKRDVDYMVKDGQVIIVDEFTGRLMPGRRYSDG
                     LHQALEAKERVRIEQENQTLASITFQNYFRMYKKLAGMTGTAATEAEEFRHIYGLDVV
                     EIPTNKPLIRIENPDVIYRTAEEKWNAVQEEIVALNATGRPVLVGTTSIEKSELLSGK
                     LRRKGIKHVVLNAKYHEQEAGIIAQAGRSGAVTIATNMAGRGVDILLGGNPEAIARET
                     LRRAGVDPAKAEAEQLENARAEARKTIGEEHDRIVGLGGLHILGTERHEARRIDNQLR
                     GRAGRQGDPGSSRFYLSLEDDLMRIFGSDRIKGLMARIGMAEGVPIEHSMVSKAIQRA
                     QKQVEGHNFTIRKHLLEYDDVMNKQRMTVYAQRKDILEGKDLKSYIEDLVEQILDWQL
                     DTHVSENKSPDEWDLDGFRNALASQFGIDAGGLGIDWNAVSFDPLREKILVHLRKIYE
                     DKERLMGAGMMRQFERFILLQIIDNQWKDHLLGMDYLKEGIGLRGYGQRDPLVEYKKE
                     SFEMFQDMLDRIEDETVRYLYLLQPAVETEMTRRPERAAYRQGPPPGGGGPKTAARAM
                     IPRKRKR"
     gene            3637..4818
                     /locus_tag="PKV64_10585"
     CDS             3637..4818
                     /locus_tag="PKV64_10585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HPN17560.1"
                     /translation="MSEPTKAKKTASGRRRAAGILLVVLIAGPVYWFGVRPDMSDFGV
                     CFRAGERLLAGETLYRETDGHLQFKYAPAAAFLFAPLSLLPLEAAKAVWFIIMAACLA
                     GILRIVSRWPAGSERAVRWAAVWTAAVMAKNLGREFQLGQVNLLLLLLMVVMVRENAA
                     GRPGGGGLSVGVSLLFKPYAAIFLPDLVLKRKWAMLAGGAAALAAGLILPAAVYGFAG
                     NAAVLAEWKNTLFASTPGLLLTGDNASLWAFAAKAFKAFGFPSTAGPLIIGGLAAAVL
                     GLLYLEMVRRGRAAAIPGSEFAEAAALMMLIPMVSPLGWNYNYLYGLPAVFLLSSALP
                     RFRPVERAVLVADFALIGGTLREVLGKTAFRFYTENALILPCFLLLFAMLYAGRRRRL
                     L"
     gene            complement(4878..>5981)
                     /locus_tag="PKV64_10590"
     CDS             complement(4878..>5981)
                     /locus_tag="PKV64_10590"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPN17561.1"
                     /translation="DPVLMEIKLKEETALREAVRRDPEIDKLYGAAWDEIAAAQKTYA
                     EIYKMYRYFEGGAGFTTSYFSTARTLVRLAAEKPKPDAERLREYRDVSLPAITRRLTA
                     ETPVFNDLEVFNLTDSLIQLQKEFGSLPEVKWLFAGRLAEDVAKDLISGTKLGDPAVR
                     KAYLEGGLQAVSLSVDPMIKLAWLVDPLSRGVRKRYEQEVESVETRNGALIAQAVFKL
                     KGTAVAPDATGTLRLSFGAVKGYVENGKKIPSFTTFKGMYDLSARNGNKDPYELAPAV
                     LSRKKKVKLATPLNFVATCDSIGGNSGSPVVNRKGELMGVLFDGNIQSLPARFVYTET
                     LNRSVMVHAAGILEALSNIYDAKPLVDEILGKK"
BASE COUNT          937 a         1825 c         1945 g         1274 t
ORIGIN      
        1 tccgggtcgt gcagatcgaa cccggcccga ccccgacctt gacggcgtcg accccgaggt
       61 cgatcagctc ccgggcggcc tcggccgttc cgatatttcc ggcgaccagc tcctgatcgg
      121 ggaattcccg gcggatcagc ttgaccatgt ccagcacccg gcggctgtgg ccgtgggccg
      181 tgtccacgac gagaacgtcg gccttggccg cgatcagggc ttgaacccgg ccgatcgcct
      241 cgtcgccgac gccgacggcc gccccgaccc ggaggcggcc cagggcgtcc ttggccgcgt
      301 tcggatactg gatccgcttg aggatgtcct tgtaggtgat catccccttg agatggtagg
      361 actcgtcgac gatcaggatc ttctcgatct tgtgctggtg gaacagcttt tccgcttcgg
      421 cgagcgacgt cccgacgggg acggtcacca gcgaccgggt catgacccgg tcgatcgtga
      481 tgtccgaacg ggtttcaaag cggatgtccc ggttggtcag gatgcccagg agcttgttgg
      541 cggcgtcggt gatcgggagg cctgaaatat ggaaatggcg catcgtgtcc cgggcctggc
      601 cgatggtgtc ggtcggccgc atgctgatgg gctcgtgaat catcccgctc tcatagcgct
      661 tgaccttatc gacttcgtcg gcctggacct cggccggcat gttccggtgg atgatcccga
      721 ggcctccctg ctgggccaag gcgatggcca tcgtcgaccg ggtcacggtg tccatcgccg
      781 ccccgacgag agggatccgg agctcaatcc ggcggctgaa ccgggtgatc gtcgaaacgt
      841 cggccggctc cacgtccgac ttggccggga gaatgaggac gtcgtcgaac gtcaatcctt
      901 cgcggatgtc ttcgtccagc atggggcctc ctcgattcaa cgctttcttt tcctcgggat
      961 catcgcccgg gcggcggttt tcggtccgcc gccgcccggc ggaggaccct ggcggtaggc
     1021 ggccctttcg ggtctccggg tcatctcggt ctcgacggcc ggctggagaa ggtagagata
     1081 gcggaccgtc tcgtcctcga tccggtcgag catatcctgg aacatctcga agctttcctt
     1141 tttgtattcg accagcggat cgcgctgacc gtagccccga agcccgattc cctccttcag
     1201 gtagtccatg cccaggagat ggtccttcca ctggttgtcg atgatctgga gaaggataaa
     1261 tcgctcgaac tggcgcatca tgccggcgcc catgaggcgt tccttgtcct cgtagatctt
     1321 gcggagatgg actaaaatct tctcccggag cgggtcgaag gagacggcgt tccaatcgat
     1381 gcccagtccg ccggcatcga tcccgaactg ggaggcgagg gcgtttcgga agccgtccag
     1441 atcccactcg tcgggcgatt tgttttccga gacgtgggtg tcgagctgcc agtcgaggat
     1501 ctgctcgacc aaatcctcga tgtacgattt gaggtccttg ccctcgagga tatccttccg
     1561 ctgggcgtac acggtcatcc gctgcttgtt catgacgtcg tcgtattcga gaaggtgctt
     1621 gcggatggtg aagttgtggc cctcgacctg cttctgggcc cgctggatgg ccttggagac
     1681 catcgagtgc tcaatgggca cgccctcggc catcccgatg cgggccatca gtcccttgat
     1741 gcggtcgctt ccgaaaatcc gcatcaagtc gtcctcgagg ctcaagtaga agcgggacga
     1801 tccgggatcg ccctggcgcc cggcccggcc gcggagctgg ttgtcgatgc ggcgggcttc
     1861 atgacgctcg gtgccgagaa tgtggagtcc gcccaaaccg acgatgcggt cgtgttcctc
     1921 gccgatggtt tttcgggcct cggcccgggc gttttcgagc tgttcggcct ccgccttggc
     1981 cgggtcgacg ccggcgcggc gaagggtttc ccgggcgatg gcctcgggat ttccgccgag
     2041 gagaatatcg acgccgcggc cggccatgtt ggtcgcgatc gtcaccgctc ccgaccggcc
     2101 ggcctgggcg atgatcccgg cctcctgctc gtgatacttg gcgttgagga cgacgtgctt
     2161 gatgcccttg cgccggagct tgccgctcaa gagctccgat ttctcgatcg acgtcgtccc
     2221 gacgaggacg gggcggccgg tcgcgttcag ggcgacgatt tcctcctgga cggcgttcca
     2281 tttttcctcc gccgtccggt agatgacgtc gggattctcg atgcggatga gcggcttgtt
     2341 ggtcgggatt tcgaccacgt ccagcccgta gatatgccgg aattcctcgg cctcggtcgc
     2401 cgcggtcccg gtcatcccgg ccagcttctt atacatccgg aaataattct ggaaggtgat
     2461 cgaggccagg gtttggtttt cctgctcgat ccggactcgt tccttggcct ccaaagcctg
     2521 gtgcagaccg tcgctgtagc ggcggccggg catgagacgg ccggtgaact cgtcgacgat
     2581 gatcacctgg ccgtccttga ccatgtaatc gacgtcgcgc ttgaagagga agtgggcttt
     2641 caaggcttgg ttcaggccgt gaaccaggtc catctgggag aggtcgtaca ggttggagac
     2701 gtgaagggcc ttttcggccg cggccacgcc ctcctccagg agcgaaacgg cccgcgattt
     2761 ctcgtcgatc ttgaaatgcg tgtcggccgc gaagcggcgg accaccgtgt cggccttctg
     2821 gtagagatcg gtcgagccct cgctcggacc ggagatgatg aggggggtcc gggcttcgtc
     2881 gatcaagatc gagtcgacct cgtcgacgac ggcgtaattg aagggccgct ggaccaggtc
     2941 cgacagccgg aatttcatat tgtcgcgcag gtagtcgaag ccgaactcgt tgttggtgcc
     3001 gtaggtgatg tcggcccggt aggcagctag gcggtcggcg tcgggcatgt catgctggat
     3061 cgtcccgacc gtcagcccga gaaagcgatg aatggggccc atccattccg tgtcccgctt
     3121 ggccagatag tcgttgaccg tgacgatatg gacgccccgg ccttccaggg cgttgaggta
     3181 cgcggggagg gtggagacga gggtttttcc ctcgcccgtc ttcatctcgg cgatttttcc
     3241 ctggtgaagg acgacgccgc cgatcagctg aacgtcgaac ggacgcatcc ccgtcgtccg
     3301 ccgggcggct tcccggacca cggcgaaggc ctcgggcaga agatcatcca aagccgcgcc
     3361 ttgagcgagc ttttcccgga attccccggt tttggctcgc agcgcgtcat cggaaagctc
     3421 tcggatcctg gattcgaaat cgttgatgag gccgacgtag ggggtgagct ttttgaggtc
     3481 ccgttcgctc ttcgttccga agagtttttc cgaaagccac ttgaccattt tctatattta
     3541 acagaagatt caggccctgt caaatacgcg gaggggaggc cgtcgccgag ctttcgcttt
     3601 gcttcggctc gctgatttct atatattaac cggcatatga gcgaacccac gaaggcgaaa
     3661 aagacggcgt ccggaagacg ccgggcggcc ggaatcctct tagtcgttct gatcgccggg
     3721 ccggtttatt ggttcggcgt ccggccggac atgtccgatt tcggagtgtg cttccgggcc
     3781 ggcgagcggc tgctggccgg ggaaaccctt taccgggaaa cggacgggca tctccagttc
     3841 aaatacgccc cggccgcggc cttcctcttc gccccgctct ccctccttcc cctcgaggcg
     3901 gccaaggccg tctggttcat catcatggcc gcctgcctgg ccggcattct caggatcgtg
     3961 tcccgctggc cggccggctc cgaacgagcg gtccgatggg cggcggtctg gaccgcggcc
     4021 gtcatggcga aaaatctggg acgggagttc cagttggggc aggtgaacct tttgcttctt
     4081 ctgttgatgg tcgtgatggt ccgggagaac gcggcgggcc ggccgggcgg gggtggactg
     4141 agcgtcgggg tgtcgctgct gttcaagcct tacgccgcga tttttctgcc ggacctggtt
     4201 cttaaaagga aatgggcgat gctggccgga ggagcggcgg ccctggcggc cggcctgatc
     4261 ctgccggcgg ccgtttacgg attcgccggc aacgcggccg ttctcgccga gtggaaaaac
     4321 acccttttcg cctccacccc cgggcttctc ctgacggggg acaatgcctc cctttgggcc
     4381 ttcgcggcta aggcctttaa ggccttcggc ttcccctcaa ccgccgggcc gttgatcatc
     4441 ggcggcctcg ccgccgcggt tctcggcctc ttgtacctgg agatggtccg ccggggccgg
     4501 gcggccgcga ttcccggctc ggaattcgcc gaggccgcgg ccctgatgat gctcattcct
     4561 atggtttccc ccctcggctg gaactataat tatctctacg ggctgccggc cgttttcctg
     4621 ttgtcgtcgg ccctgccgcg ctttcggccg gtcgagcggg cggtcctcgt cgctgatttc
     4681 gccctcatcg gaggaacgct gcgggaagtc ctgggaaaga cggctttccg gttttacacg
     4741 gaaaacgccc tgatcctgcc ttgttttctt cttctctttg cgatgctgta cgccgggcgg
     4801 cggcgccggc ttctgtaaaa cggataaaac gggggggcac ggcttccgcc gcgccccccg
     4861 gatcgatcat gccccgatta cttcttcccc aagatctcgt ccaccagggg cttggcgtcg
     4921 tagatgttcg acagggcctc gaggattccg gcggcatgga ccatcaccga ccggttgagc
     4981 gtctcggtat agacgaaacg agccggaagg ctttggatgt tgccgtcgaa gagaacgccc
     5041 atgagctcgc ctttccggtt gacgaccggc gagcctgaat tgccgccgat cgaatcgcac
     5101 gtcgccacga aattgagcgg ggtggcgagc ttgacctttt ttttccggga aagaacggcc
     5161 ggggcgagct cgtagggatc cttgttgccg ttcctcgccg agaggtcgta cattcccttg
     5221 aaggtcgtga acgaagggat tttcttcccg ttttcgacat atcccttgac cgccccgaag
     5281 ctgagacgga gcgtgcccgt ggcgtcgggc gcgacggccg ttcccttcag cttgaagacg
     5341 gcctgggcga tcagggcgcc gttgcgcgtt tcgacggatt ccacttcctg ctcgtaacgt
     5401 ttccggacgc cccgggacaa gggatcgacg agccaggcca gcttgatcat cggatcaacg
     5461 gacagggaga cggcttggag gcctccctcg aggtaggctt tccggaccgc gggatcgccg
     5521 agcttcgttc cggagatcaa gtccttggcc acatcctcgg ccagccggcc ggcgaacagc
     5581 cacttgacct cgggcaggga gccgaattcc ttctggagct ggatgaggga atccgtcagg
     5641 ttgaaaactt caagatcgtt gaaaacgggt gtttcggccg tcagccggcg ggtgatggcg
     5701 gggagggaga cgtcccggta ctcgcgcagc cgttccgcgt cgggcttggg cttttcggcg
     5761 gccaggcgga cgagcgtccg ggcggtggag aagtaagagg tcgtgaaccc ggctccgccc
     5821 tcgaaatagc ggtacatttt ataaatttcg gcataggtct tctgggccgc ggcgatctcg
     5881 tcccaggccg ctccataaag cttgtctatc tcgggatccc ggcggacggc ctcccgaagg
     5941 gcggtttcct ccttgagctt gatctccatc agaacgggat c
//
LOCUS       DAOOXH010000121         5945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_15417_length_5945_cov_5.297453, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000121 DAOOXH010000000
VERSION     DAOOXH010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15417_length_5945_cov_5.297453"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..896
                     /locus_tag="PKV64_10595"
     CDS             129..896
                     /locus_tag="PKV64_10595"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN17562.1"
                     /translation="MNLSRRAFLVFYRNLVSYKRFVVPTLVGSLVQPVFYLVTFGIGM
                     GAYMGAFGGKSYLFFLVPGVLISSVMMSSTFECLYGAFVKMIHEKLYDSLIATPVSAE
                     DAVAGDILWGAFRGLISGGLMMSVAFFMGVFPVSVPAGLLLCVLMVLIGVMFGSLAMI
                     VTSYAPNFDFFNYYTELVMTPMLFFSGVFFPLDRFPAWMKTLAQFLPLTHAVEISRAV
                     YSGVYPKSLVLNLAFVLVLTFAAVRVGVGRMKKRLIK"
     gene            complement(909..2291)
                     /gene="rmuC"
                     /locus_tag="PKV64_10600"
     CDS             complement(909..2291)
                     /gene="rmuC"
                     /locus_tag="PKV64_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPN17563.1"
                     /translation="MSLFQIIGLVTAVLAFIAVVLLLVFFKRPRAGADLSLLQARLDA
                     LERGQERGERELRDEFVRNREESSRHLSTLRQEIGTSLKNVSDSVFGQVSGLTKFQQA
                     QMEAFGDRLGKLTEASEKKADALRDAVEKKLDRIQANNDARLEEMRKTVDEKLQTTLE
                     KRLGESFKFVSDRLDQVNKGLGEMQTLASGVGDLKKVLTNVKTRGVWGEIQLGNLLEQ
                     FLTADQYERNVKPKAGSDEAVEFVIKFPGRSDDPDNPLLLPIDAKFPKEEYERLVDAS
                     ERADVEAVEDSSRKLEAQIKAEAKKIRDKYLDPPRTTDFGVMFLPTEGLYAEVLRRPG
                     LTDVLQREFRVIVAGPTTLVALLNTFQMGFQTLAIEKRSAEIQTLLGAVKTEFGKFGD
                     VIEKVHKKIMDAGDVIESAKTRSRAIERKLRRVEALPAAEAGAILALPNGEGDEEPEP
                     VKAEGEEPTA"
     gene            complement(2619..3851)
                     /locus_tag="PKV64_10605"
     CDS             complement(2619..3851)
                     /locus_tag="PKV64_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HPN17564.1"
                     /translation="MDLFEKCRDFYSNPESAPKYGYPMTPRAAMAAGLFPFFIPIDQA
                     EGPEAIIDGKHYIMIGSNNYLGLTVHPKVKEAAAEAVRKYGTSCTGSRFLNGTLHMHI
                     ELEEKLADYVGQEAALVYSTGFQVNLGSIPAIMDHDDIIITDKDVHASIVDGVRMAKV
                     QKGVHIRYFKHNDAADLDKILKTLPPEPGKLVIIDGVYSMGGDIAPLPEIVRVCKAHG
                     ARLFCDDAHALGVLGNGRGTGAHFGMEKDVDLVMGTFSKSFASVGGFIAGPKEAVAWI
                     QMFSRPFIFSASLPPANVAAVLACLDVLREEPDRVARLEANGKRMRTELRAMGYNIGP
                     SETPIIPIVIGDQFKALKAWKVFFDEGIYANVALPPAVTPEFSCLRTSYMATHTDAQL
                     DRVLDVFDKVKHQIHIGE"
     gene            complement(3984..5078)
                     /gene="metK"
                     /locus_tag="PKV64_10610"
     CDS             complement(3984..5078)
                     /gene="metK"
                     /locus_tag="PKV64_10610"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPN17565.1"
                     /translation="MSMYEFTSESICEGHPDKVCDAIADGILDACLAGDKRSRVACEV
                     LCKDGQVVLAGEITTAADPDYEKITRETITRIGYTDASEPFSAESVAVRSLLSRQSGE
                     IAQGVNAAEQGAGDQGIMFGYATDETPEMMPLPILLAHRLAEGMAEDRKSGREPWLRP
                     DGKTQVTVRYEDGRPAAVTTVLVSAQHRAEMSRDGIRAYVAERLGPRVLNSWWNEKIS
                     LFVNPTGSFVLGGPSADCGVTGRKIICDTYGGAARHGGGAFSGKDPSKVDRSAAYFCR
                     FIARQVVKQGLARKAEIQVSYAIGEAKPLSVFVETFGTGDNAAAAAFVRKNFDFRPAA
                     IIERLDLLRPIYGRTTNYGHFGKPGLPWEA"
     regulatory      complement(5160..5292)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            complement(5420..>5945)
                     /locus_tag="PKV64_10615"
     CDS             complement(5420..>5945)
                     /locus_tag="PKV64_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17566.1"
                     /translation="GKYGLMAAMRLSNGLEIISPIPGGLSRGENETILKGPEEGAPLV
                     REAAERLGLAGRPLGSIGDVFEIERLIMTMDVERARAKGILRPFADAEWEAYEMEAGF
                     KRMIGGDVALTLSQLLIKAVALRDGLPPWLVYRNLGLALNKKIGMSFDNYPEARRLFY
                     EPIFAGTAGAGAFL"
BASE COUNT          953 a         1885 c         1893 g         1214 t
ORIGIN      
        1 ttcttctatt gccgcgacgg ccgcgatatc cagaagaagc tggtcgagct cgacctgccc
       61 aacaccgtcc accggccggc caccctggag gacgtctttc tgaagctcac gggaaggagc
      121 ctcatcgaat gaacctttcc cgccgggcct ttctcgtctt ctaccgcaac ctggtttcct
      181 acaagcggtt cgtcgtcccg acgctcgtcg gcagcctggt ccagccggtg ttctacctgg
      241 tgaccttcgg catcggcatg ggcgcctaca tgggcgcctt cggcgggaaa tcctatctct
      301 tcttcctcgt ccccggcgtc ctgatctcct cggtgatgat gtcctcgaca ttcgagtgcc
      361 tctacggcgc cttcgtcaag atgatccacg agaagctcta cgattcgctg atcgccaccc
      421 ccgtctcggc cgaggacgcc gtcgccgggg acatcctctg gggcgccttc cgcggcctga
      481 tcagcggcgg cctgatgatg agcgtggcct ttttcatggg ggtgtttccg gtctcggttc
      541 cggccgggct tctcctctgc gtcctgatgg tcctgatcgg cgtcatgttc ggctcgctgg
      601 ccatgatcgt cacgtcgtac gcgcccaact tcgacttctt caattattac accgagctgg
      661 tcatgacccc catgctcttc ttttcgggcg tgtttttccc cctggaccgg ttccccgcct
      721 ggatgaagac gctggctcag tttctccccc tgacccatgc cgtggaaatc tcgcgggcgg
      781 tttactcggg cgtctatccg aaaagcctcg tcctgaatct cgccttcgtc ctggtcctga
      841 ctttcgccgc cgtccgggtc ggcgtcgggc ggatgaagaa gcggctgatc aagtaatccg
      901 cgcgttcgtc aggcggtggg ctcttccccc tcggctttca ccggttccgg ctcctcgtcc
      961 ccctcgccgt tgggcagggc caggatcgcc cccgcctcgg cggccggaag cgcctccact
     1021 cgccggagct ttcgttcgat ggcccggctc cgggtcttgg ccgactcgat gacatcgccg
     1081 gcgtccatga tcttcttgtg aaccttctca atcacgtcgc cgaatttccc gaactccgtc
     1141 ttgaccgccc ccagcagcgt ctggatctcg gccgaccgct tctcgatggc cagcgtctgg
     1201 aagcccatct gaaaggtgtt gaggagggcg accagggtcg tcggcccggc cacgatgacc
     1261 cggaattcgc gctggaggac gtcggtcaag ccggggcgcc ggagcacctc ggcgtagagg
     1321 ccctccgtcg gcaggaacat caccccgaaa tcggtcgtgc ggggcgggtc gaggtacttg
     1381 tcccggattt ttttcgcctc ggccttgatc tgggcctcga gcttgcggct ggaatcctcg
     1441 acggcttcca cgtccgcccg ctcggaggcg tcgaccaggc gctcgtattc ctccttgggg
     1501 aacttggcgt cgatcgggag caggagggga ttatcgggat cgtcgctccg gccggggaac
     1561 ttgatgacga actcgacggc ctcgtcgctt ccagctttgg gtttgacgtt ccgctcgtac
     1621 tggtcggccg tcaggaactg ctcgagaagg ttgccgagct ggatttcccc ccacacgccg
     1681 cgggtcttga cgttggtcag gactttcttc aaatcgccga cgccgctggc cagggtctgc
     1741 atctccccca ggcccttgtt gacctggtcc aggcggtcgc tgacgaactt gaaggattcg
     1801 cccaggcgct tctccagcgt cgtctggagc ttctcgtcca ccgttttgcg catttcctcc
     1861 aggcgggcgt cgttgttggc ttggatccgg tcgagcttct tttcgacggc gtcccggagg
     1921 gcgtcggctt ttttctcgct cgcctcggtc agcttgccca ggcggtcgcc gaaggcctcc
     1981 atctgggctt gctggaactt ggtcaagccg gagacctggc cgaagacgga atcggagacg
     2041 ttcttgagcg acgtcccgat ctcctggcgg agagtggaga gatgccggct cgattcctcc
     2101 cggttgcgga cgaactcgtc gcgcagctcc cgctcgcccc gctcctgccc cctctcgagc
     2161 gcgtcaagcc tcgcctggag gagcgaaaga tcggcgcccg cccggggacg tttaaagaaa
     2221 acgaggagaa gaacgacggc gatgaacgcc aggacggccg tgacgaggcc gatgatctgg
     2281 aacaaggaca tggctcaacc tccgctgaca ttcagataac agaacgaccg ggtgaagtca
     2341 aaccggcgga tccttgtgtc aaggcaaggt gaacatgccc ggtttagcct aagggtccga
     2401 tccttttgag atcggttttg tcgtgaagag agggctcatg gcggcgccgg ggggggtcgg
     2461 gcggagggat ggacaccggc tttgacgttt gaaaacagcc ggagcggctt tgggggaggt
     2521 ttttcggccg gctcttgagt gatgtcccgg acaacggacc gccggacccg cctttgagga
     2581 aagcggccgg ccgccgcttc gtgttttttc ggcccgtcct attcgccgat gtgaatctgg
     2641 tgcttcacct tgtcgaagac atccagcacc cggtcgagct gggcgtcggt gtgggtggcc
     2701 atgtaactgg tccggagaca ggaaaactcc ggcgtgaccg ccggcggcag ggcgacattg
     2761 gcgtagatgc cctcgtcgaa gaagaccttc caagccttga gggccttgaa ctggtcgccg
     2821 atgacgatgg ggatgatggg cgtttcgctc gggccgatgt tgtagcccat cgcccgcagc
     2881 tcggtccgca tccgcttccc gttggcttcc aggcgggcga cccggtcggg ctcctcccgg
     2941 aggacgtcga ggcaggccag gacggcggcc acgttggccg gcggcaggct ggcgctgaaa
     3001 atgaacggcc gggagaacat ctggatccag gccacggctt ccttcgggcc ggcgatgaat
     3061 ccgccgacgg aagcgaagga cttgctgaac gttcccatga ccaggtcgac gtccttttcc
     3121 atcccgaaat gggctcccgt cccccgtccg ttgccgagga cgcccagggc gtgggcgtcg
     3181 tcgcagaaga ggcgggcgcc gtgggccttg cagacgcgga cgatctcggg caggggggcg
     3241 atgtcgccgc ccatgctgta cacgccgtcg atgatgacga gcttgcccgg ctcgggcgga
     3301 agggttttca agatcttgtc gaggtcggcg gcgtcgttat gcttgaaata gcggatgtgg
     3361 acgcctttct ggaccttggc catccggact ccgtcgacga tgctggcatg gacgtccttg
     3421 tcggtgatga tgatatcgtc gtggtccatg atcgccggga tggaccccag gttgacctgg
     3481 aagcccgtgc tgtagaccag ggcggcctcc tgaccgacgt aatcggccag cttttcctcg
     3541 agctcgatgt gcatgtggag cgtgccgttg agaaaccggg acccggtgca gctcgtgccg
     3601 tacttgcgga cggcctcggc cgcggcttcc ttgaccttcg gatgaaccgt caggccgagg
     3661 tagttgttgg agccgatcat gatgtagtgc ttgccgtcga tgatcgcttc cggcccctcg
     3721 gcctggtcga tcggaatgaa aaagggaaag agcccggcgg ccatggccgc ccgcggcgtc
     3781 atcggatacc cgtatttggg ggcgctttcc gggttggagt agaagtcccg gcatttttcg
     3841 aaaagatcca tgtcgtcctc tctctttctc tatttcctat cgagccaaga acaagatgtg
     3901 atggagcttc tgcttaaaac gagtaggatt tataaactat atggccggac ttgtcaattt
     3961 tcgccgcttt tccggtggat ttttcaggct tcccagggca gccccggctt cccgaaatga
     4021 ccgtagttgg tggtccggcc gtaaatcgga cgcagaaggt cgagccgctc gatgatggcc
     4081 gccgggcgga agtcgaaatt tttcctcacg aatgcggcgg ccgcggcgtt gtctcccgtg
     4141 ccgaacgtct cgacgaacac cgaaagcggc ttggcctcgc cgatggcata ggaaacctgg
     4201 atttccgcct tccgggccag cccctgcttg acgacctggc gggcgatgaa ccggcagaaa
     4261 taagccgccg agcggtccac cttgctggga tccttgccgc tgaaggcccc tccgccgtgg
     4321 cgggcggcgc cgccgtacgt gtcgcagatg atcttgcgcc ccgtgacgcc gcaatcggcc
     4381 gaggggccgc ccaggacgaa gctgcccgtc ggattgacga agaggctgat tttctcgttc
     4441 caccaggaat tcagaacccg ggggccgagc cgttcggcga cataagcccg aatcccgtcc
     4501 cggctcattt cggcccggtg ctgggccgag accaggacgg tcgtgacggc cgccggccgg
     4561 ccgtcttcgt agcggaccgt gacctgggtt ttcccgtcgg gacgcagcca cggctcgcgg
     4621 ccgcttttac ggtcctcggc catcccctcg gccaggcgat gggccaggag gatgggaagc
     4681 ggcatcatct ccggggtttc gtcggtcgcg tacccgaaca tgatgccctg atcgccggcg
     4741 ccctgctcgg cggcattgac gccttgggcg atttccccgg actggcggct caacaaggag
     4801 cggacggcga cggactcggc ggaaaagggc tcggacgcgt ccgtgtagcc gatccgggtg
     4861 atcgtctccc gggtgatttt ctcgtaatcc ggatcggccg ccgtggtgat ttccccggcc
     4921 aggacgactt ggccgtcctt gcacaggacc tcgcaggcca cgcggctgcg cttgtccccc
     4981 gccaggcagg cgtccaggat gccgtcggcg atggcatcgc agaccttgtc cggatgtccc
     5041 tcgcagatgg attccgaagt gaactcgtac atcgacatga tgcgtctcct ttcgcgctta
     5101 cgagcgcaaa cgtcatacgc atcaggaggg gtgaattcgc ccgcggcgga aaaccgcggt
     5161 gagagacttt gattttggaa gccgccttcc acacatcctc gccgccgcgg ggcggcgagt
     5221 aagcaccttg gtgtctctcc aaaccaggtt gccgcacccg gcggacaagc cgggtggctc
     5281 ctgatgcatc ccgcattaaa gcaaaagccg gctcctctgt caaccgttgt aggctccatg
     5341 acttagagtg tgtggaataa ggggcaagtg cggctgaggt ccccggaagc ccgagcccgg
     5401 agaggcgggc cggattaaat caaaggaagg cccccgcccc ggccgtcccg gcgaagatcg
     5461 gctcatagaa gagccgtcgg gcttccgggt aattatcgaa gctcattccg attttcttgt
     5521 tcaaggccag gccgagattc cggtagacga gccagggagg gaggccgtcc ctcaaggcca
     5581 cggccttgat caaaagctgg ctgagggtca gggcgacgtc gccgccgatc atccgcttga
     5641 agcccgcttc catctcgtaa gcctcccact ccgcgtccgc aaacggccgg agaatccctt
     5701 tggcccgcgc ccgctcgacg tccatggtca tgatcaggcg ctcgatctcg aagacgtcgc
     5761 cgatcgagcc gagcggccgg ccggccaggc ccagtctttc ggccgcctcc cggacgagcg
     5821 gcgcgccctc ctccggcccc ttgaggatgg tctcgttctc gccgcgggac aggccgcccg
     5881 gaatgggcga tatgatttcc agcccgttgg agaggcgcat cgcggccatc agcccgtatt
     5941 tcccc
//
LOCUS       DAOOXH010000122         5782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_16026_length_5782_cov_8.185612, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000122 DAOOXH010000000
VERSION     DAOOXH010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16026_length_5782_cov_8.185612"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="PKV64_10620"
     CDS             complement(<1..1376)
                     /locus_tag="PKV64_10620"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HPN17567.1"
                     /translation="MAGRRSIFTVSALAGIVIFLAALAGSAAETSAQSRPQTGSQAVP
                     PQAAAPSPAETAVGKLVIKADYKERLDNKITASGNVEIRYGEIILCADQVVYDETTKD
                     ALAVGHVTLIRPDETFSADRFALNMETGLGRAENVVGLVQPEYRYESDRVERESPDLF
                     RVGKSTITGCSQPVPRWKFSASRAEFVRNEYIKMWDPVFKIKDVPIFYLPYLKYPLDR
                     DRATGFLFPRIGYSKQKGLNLTQQFYWAMARNMDATFSLDYYAAKGAGGGLEFRYLFE
                     GGWGGRLNAFYFGYRTPSTGVKPEDAVIFRWSHNQPLPGKLNIVANVDYQNSFSFTRE
                     FDNDFTRALVYNRSSQVYVTKSWTGSNLSVRLSRFETTYPAWTFARIRQSLPQISFSL
                     FKKKLWGPSYFSLSAGYDNWQYGFTNQFKAGTQVKSSVLSLSPRITLPFTEIAWLGFD
                     FSVAGNLD"
     gene            complement(1479..2444)
                     /locus_tag="PKV64_10625"
     CDS             complement(1479..2444)
                     /locus_tag="PKV64_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008949512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPN17568.1"
                     /translation="MQEFIPSLKRFALIGAAGYIAPRHLKAIKDTGNVLVAAMDKSDS
                     VGVLDSYFPGASFFTEFERFDRFLEKLRRADPAGGIDCLSIATPNYLHDAHIRFALRL
                     GASAICEKPVVLNPWNLDALKAIEAEYGRKVYTVLQMRLHPALRALKSRVETGPAGRV
                     YDVELTNVTARGRWYFYSWKGDEAKSGGVATNIGIHFFDVLTWIFGEAVAGGNIVHLK
                     EPDRAAGFLELRRARIRWYLSLRAGDVPAAGRARSPRAFRRITVDGEPADFTEGCADL
                     HSRVYEEILAGRGFGLEENRRAVEIVHDIRQAAVDPRRGERHPLL"
     gene            complement(2542..5145)
                     /locus_tag="PKV64_10630"
     CDS             complement(2542..5145)
                     /locus_tag="PKV64_10630"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HPN17569.1"
                     /translation="MKPFLLLAELVPAQGDDVRPIECFLRDYAGRKSEIPSGFRLAGV
                     ALPQNPRGIPMLDPADIFSIIAAKGPSGRGLWDDLDVVPHVTGKEQNAEALRSALMGF
                     RAMGLTTVLAITGDKPASGKRVFELDSIGLLELVSELNFDAFQRAPVSPPAKRTVPSP
                     EHPPAPGGFSRIGEMLAGPRPGSASFASRFSSVPQFFALAAVSPFKYTEASLLQQYFK
                     AAKKLQAGAQAFLLQMGWDSRKSEELFRYFRQARLDAPVLGNVYYLSNANPAARLMNE
                     GRMPGCFVSDELLAAVRREKAEAHLERAACQVAMYRDLGADGVDLGGLPDFDALVRIA
                     VRAGEIGPGWRQRRIILDYPPPPPASGPGLFYLYGPETEGGSAPRPPFRPSPRPRTFR
                     QRRFDFLHRNFLTKGKRLCPAVAGTARAFGLEKGEGSLYRWFHGAEAAAKAVLFACED
                     CGDCFLPENFGYCTLGECAKGLPNPPCGDATPEGRCGNDETRVCVAEPIFQAAAAAGK
                     LDGLAETALPPRIAALEHTSGVLNYVFDRDHARGFPLIQIAELLHASIPRTAAAVQEV
                     IKEGLIGAPPGAVRASGSAALGYLVSLIRRQAKQGAAYIDVNVDALSEDDPEFRKEIL
                     RFFVRLIRGHSLGVPVCIDSGSTEMLEAGLAAWYEGGREGGRPSAAPLVNSVKTYTME
                     RLLPLRARFPFKFIGLLVDEKTAGLDGAYGVEELQALARRIVRAATGSHGFAPGDIFL
                     DSTVFPLSIDVPMEAAKPGYTFRAFETIRRLKADPEFRGVHFSLGVTNAVRDLPGRRT
                     GVTRAYLARAMELGLDAAIVNVFHGYGRRPPAPDLLAFVDAFARQDGSPEAVENALQA
                     MMDFCRANRKK"
     gene            5266..>5782
                     /locus_tag="PKV64_10635"
     CDS             5266..>5782
                     /locus_tag="PKV64_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HPN17570.1"
                     /translation="MGKADPGKAPAETPYFVHPTSVVDPGATIGAGTKIWHFSHVQTG
                     ARIGKHCVLGQNVNIGENATVGDFVKIQNNVSVYSGVILEDYVFCGPSLVFTNVAVPR
                     SKYPRPGPEHYQPTLVKEGASLGANATIVCGHTIGRHAFVGAGAVVTRDVPDFALVVG
                     NPARIAGWMSEA"
BASE COUNT         1082 a         1777 c         1956 g          967 t
ORIGIN      
        1 taatcgaggt tgccggcgac ggagaaatca aaaccgagcc aagcgatctc ggtgaagggc
       61 agggtgatcc tcggcgaaag ggacaggacg ctcgatttca cctgggtgcc cgccttgaac
      121 tggttggtga atccatactg ccagttgtcg tagccggcgc tcaaggaaaa atagctcggc
      181 ccccagagct ttttcttgaa caggctgaag ctgatttgcg gaagggattg gcggatccgg
      241 gcgaacgtcc aggcggggta ggtcgtctcg aaccggctga ggcggacgct caaattcgat
      301 ccggtccagc ttttcgtcac gtagacctgg gagctccggt tataaaccag ggcccgggtg
      361 aagtcgttgt cgaactcccg ggtgaaggag aaggagttct ggtagtcgac gttggcgacg
      421 atgttgagct ttccgggaag aggctggttg tggctccagc gaaagatgac ggcatcttcc
      481 ggcttgacgc ccgtcgacgg agtccggtag ccgaaataga aggcgttgag gcggccgccc
      541 catccgccct cgaacaggta gcggaattcg aggcccccgc cggcgccctt ggccgcgtaa
      601 taatccaggg aaaaggtggc gtccatattc cgggccatgg cccaatagaa ctgctgggtc
      661 aggttcaagc ctttctgttt cgaataaccg atccggggga agagaaagcc cgtggcccgg
      721 tcccggtcaa ggggatattt gaggtaggga agatagaaga tcggaacgtc cttgatcttg
      781 aagaccggat cccacatctt aatgtattcg ttccgcacga attcggcccg ggaggcggag
      841 aacttccagc gggggacggg ctgggagcag ccggtgatcg tgcttttccc cacccggaac
      901 aggtcggggc tttcgcgctc gacccggtcg gactcgtacc gatattcggg ttgaacgagc
      961 ccgacgacgt tttcggcccg gcccaggccg gtttccatgt tcaaggcgaa ccggtcggcc
     1021 gagaacgtct cgtcgggccg gatcagggtg acgtggccga cggccagggc gtccttggtc
     1081 gtctcgtcat agacgacctg gtcggcgcag agaatgatct cgccataacg gatctccacg
     1141 tttccggagg cggtgatttt gttgtcgagc cgttccttgt agtccgcctt gatgacgagc
     1201 ttgccgacgg cggtttcggc cggggacggc gcggccgcct ggggcggaac ggcctgagac
     1261 ccggtctgcg gccgggactg ggccgaagtc tcggccgcgg acccggccag ggcggcaagg
     1321 aaaatcacaa ttccggccag ggcggaaaca gtaaaaatgc tccgccggcc cgccattatt
     1381 ttccttcctg gcaattgcat gggaccgccc aagcctcata ttgtagcaca acggaagcgg
     1441 cttcgattcc gccgcgacgg gggatgcctg gccctgaatc agaggagcgg gtggcgttcg
     1501 ccccggcggg gatcgacggc ggcctgccgg atgtcgtgga cgatctcgac cgcccggcgg
     1561 ttttcctcga ggccgaagcc gcggccggcg aggatctcct cgtaaacgcg ggaatgaagg
     1621 tccgcgcagc cctcggtgaa gtccgcgggc tccccgtcga ccgtgatccg gcggaacgcc
     1681 cgcgggctcc gcgcacggcc ggcggcggga acgtccccgg cccggagcga gagataccac
     1741 cggatccggg cccggcggag ctccaggaag ccggccgccc tgtccggctc cttcaagtga
     1801 acgatattgc cgccggcgac cgcctccccg aagatccacg tcaggacatc gaagaaatgg
     1861 atgccgatgt tggtcgcgac tccccctgat ttcgcctcat cccctttcca ggaatagaaa
     1921 taccagcggc cgcgggccgt gacattggtc agctcgacgt cgtaaacccg cccggccggg
     1981 ccggtctcga cccgcgactt cagcgcccga agggccgggt ggagccgcat ttggaggacg
     2041 gtatacacct tccggccgta ctcggcctcg atggctttca aggcgtcgag gttccaagga
     2101 ttcaagacga cgggcttttc gcagatcgcc gaggcgccca ggcggagagc aaaccggatg
     2161 tgggcgtcat ggaggtaatt gggcgtggcg atgctcaggc aatcgatccc tccggccgga
     2221 tccgcccgcc ggagtttttc caggaaacga tcgaaacgct cgaactcggt gaaaaaagac
     2281 gccccgggaa aataggaatc gagcacgccg acggaatcgg atttgtccat cgccgcgacg
     2341 aggacgttgc cggtgtcctt gatcgccttc aagtgccggg gggcgatata gccggcggcc
     2401 ccgatcaggg cgaatcgctt gagcgagggg atgaattcct gcatcgacgg cttctccttc
     2461 cgtctccggg ctccggagaa aatcatatcg aaaaagaaac cggagctcaa ctcgaaccct
     2521 ggacaggcag gcgcccgtgg attatttttt ccggttggcc cggcaaaaat ccatcatcgc
     2581 ctggagggcg ttttccaccg cttccggcga gccgtcctgg cgggcgaacg cgtcgacgaa
     2641 ggccaggagg tcgggggcgg gcggacgccg gccgtagccg tgaaagacgt tgacgatcgc
     2701 cgcgtccagg ccgagctcca tggcccgggc caggtaggcg cgggtgacgc ccgtccggcg
     2761 gccgggcaag tcgcggacgg cgttggtcac gcccagggaa aaatgaaccc ccctgaactc
     2821 gggatcggcc ttgagcctcc ggatcgtctc gaacgcccgg aacgtatagc ccggtttggc
     2881 cgcctccatc gggacgtcga tcgagagggg gaagacggtc gaatccagaa agatatcgcc
     2941 cggggcgaat ccgtgcgatc cggtcgcggc ccggacgatc cgccgggcca gcgcttgaag
     3001 ctcctcaacg ccgtaggcgc cgtcgagacc ggccgttttc tcgtctacga ggagaccgat
     3061 gaacttgaag gggaagcggg cgcgcagcgg gagcagccgc tccatcgtgt aggttttgac
     3121 ggagttgacg agcggggcgg ccgacggccg gccgccttcc cgcccgccct cgtaccaggc
     3181 ggccaagccc gcctccagca tttccgtcga gccgctgtcg atgcagaccg ggacgcccaa
     3241 ggagtgtccg cggatcaggc ggacgaaaaa gcgcaggatc tccttccgga attccggatc
     3301 atcctcgctg agggcatcga cgttgacgtc gatataggcc gccccctgct tggcctgccg
     3361 ccggatgagc gaaacgagat acccgagggc ggcggagccg gacgcccgga cggccccggg
     3421 cggggcgccg atgaggccct ccttgatcac ctcctggacg gccgccgccg tccgggggat
     3481 gctggcgtgg agcagctcgg cgatttggat cagcggaaag ccccgggcgt ggtcgcggtc
     3541 aaacacgtaa ttcagaacgc cggaggtgtg ctcgagggcg gcgatccgcg ggggaagcgc
     3601 ggtttccgcc aagccgtcga gctttccagc cgcggcggcc gcttggaaga tcggctcggc
     3661 cacgcagacg cgcgtctcgt cgtttccgca gcggccttcc ggcgtggcgt cgccgcaggg
     3721 aggattgggc agccccttgg cgcattcccc gagcgtgcaa tagccgaagt tttccggcag
     3781 gaagcagtct ccgcagtcct cgcaagcgaa aagaacggcc ttggccgccg cttccgctcc
     3841 gtgaaaccac cggtaaaggc tcccctcccc tttttcgagt ccgaaggccc gggcggtccc
     3901 ggcgaccgcc ggacagaggc gctttccctt cgtcaagaag ttccggtgaa gaaaatcgaa
     3961 ccgccgctgg cggaacgtcc ggggccgcgg ggacggccgg aacggcggcc ttggggcgga
     4021 acctccctcc gtctccggtc cgtagaggta gaacaatccc ggcccggaag cgggcggggg
     4081 cggcggataa tccaagatga tccggcgttg gcgccagccc ggaccgatct cgccggcccg
     4141 aacggcgatc cggaccaggg cgtcgaaatc gggcaaaccg ccgagatcga cgccgtcggc
     4201 tccgagatcg cggtacatcg cgacctggca ggcggcccgc tcgagatgag cctccgcttt
     4261 ctcccgccgg acggcggcca ggagctcgtc ggaaacgaag cagccgggca tccggccctc
     4321 gttcatcagc cgggcagccg ggttggcgtt ggagagataa tagacattgc cgaggacggg
     4381 cgcgtcgagg cgggcttggc ggaaataccg aaagagctcc tcggacttgc gggagtccca
     4441 gcccatctgc agaagaaagg cttgagcgcc ggcctggagt tttttggccg ctttgaaata
     4501 ttgctgaagg agcgaggcct cggtgtactt gaacggagaa accgcggcca gggcgaaaaa
     4561 ttgagggacc gaggaaaatc tggaggcgaa ggaggccgaa cctgggcgcg ggccggccag
     4621 catctccccg attctcgaga agccgccggg ggcgggcgga tgctccgggg acggaaccgt
     4681 ccgcttcgcc ggaggggaga ccggagctcg ctgaaacgcg tcgaaattca gctcggagac
     4741 gagctcgagc aggccgatgg agtccagctc gaagacccgc tttccggacg ccggcttgtc
     4801 gccggtgatc gccaggaccg ttgtcaggcc catggcccgg aagcccatca gggcggaccg
     4861 gagggcctcg gcgttctgtt cctttccggt gacatgggga acgacgtcga gatcatccca
     4921 cagtccccgg cccgacggcc ccttggccgc gatgatcgaa aagatgtcgg ccggatcaag
     4981 catgggaatg ccccgcggat tctgcggaag agcgacgccc gccaaacgga agccggaggg
     5041 gatttcagat tttcggccgg cgtaatcccg aagaaaacat tcgatcggcc ggacgtcgtc
     5101 cccctgggcg gggacgagct cggccagcag taaaaacggt ttcattcaaa ccttttcctt
     5161 ggaaagtcgg gctttatgat aaacgatcgc tcccggactt acaacgtatt gattccccgg
     5221 ccggcatgaa ttgaggaggg gccgtgtttc tggtatattt tcgtcatggg aaaagccgat
     5281 ccggggaaag cgcccgccga aacgccctat ttcgttcatc cgacttcggt cgtcgatccc
     5341 ggggcgacga tcggtgccgg aacgaagatc tggcattttt cccacgttca aaccggcgcc
     5401 cgaatcggaa agcactgcgt cctcgggcag aacgtcaaca tcggcgagaa cgcgacggtc
     5461 ggcgatttcg tcaagatcca gaacaacgtc tcggtctatt cgggcgtcat cctcgaggat
     5521 tacgtcttct gcgggccgtc cttggtgttc accaacgtcg ccgtcccgcg gagcaaatat
     5581 ccccggcccg gcccggagca ctaccagccc acgctcgtca aggagggcgc ctcgctcgga
     5641 gccaacgcga cgatcgtctg cggccatacg atcggccggc acgccttcgt cggggccgga
     5701 gcggtggtga cgagggacgt tcccgatttc gccctcgtcg tcggcaatcc ggcccggatc
     5761 gccggctgga tgagcgaggc cg
//
LOCUS       DAOOXH010000123         5757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_16108_length_5757_cov_7.109786, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000123 DAOOXH010000000
VERSION     DAOOXH010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16108_length_5757_cov_7.109786"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PKV64_10640"
     CDS             <1..302
                     /locus_tag="PKV64_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17571.1"
                     /translation="SAECHVIRTPESGTYQYAVCVEDEDYGWGTVKYSKGGTAYAAYP
                     IVRIYKGNTLIKVINSSAATRVGTGTLYWSVFNLNAATGAVTVINKITDTKPFYF"
     gene            complement(392..1651)
                     /locus_tag="PKV64_10645"
     CDS             complement(392..1651)
                     /locus_tag="PKV64_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17572.1"
                     /translation="MKRNSGVRFLAAAVLAGAAGLWAQTEAKTPAFGISFSGFLKTDL
                     IYDSRQTVAIREGHYLLYPKGEAPGPDGRDVNAASSFHMLSVQTRLLGRITGPDAFGA
                     KTSGLIEAEFFGTSDGDLNGFRLRHGFLKLAWPKAELLIGQYWHPMFITDSFPDVVSF
                     NTGAPFQPFNRSPQIRYTRKLGERWSVSAAALAQRDFASTGPEGASTAYARNAAVPEV
                     NLTLQYRRTCEAGCESVFGIGGDYKRIVPRLATTLGYRTDAGLNDWAGMAFGKLKKPG
                     FTVKAEAVYGQNLHHLTMMGGYAAAEITDAVRAEASYAPTENLALWGEIQTNGAAFQA
                     GLFAGYTKNFGFGRNVSGPLYERGADIDAVFRISPRAVYNAGKVRLAVEAEYTAAAYG
                     KTGPDGRVNGAAWVGNLRLLGAVYYFF"
     gene            complement(1662..4349)
                     /locus_tag="PKV64_10650"
     CDS             complement(1662..4349)
                     /locus_tag="PKV64_10650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPN17573.1"
                     /translation="MNIHQLDGFFNPQRIALIGVTVNPNSVGGKILGNLVGSGFLGVV
                     YPVNPAVEAVMGIPCYPGLRSLPRTPDLGILCGPAAEVPGLVRECGEAGLRSLVIVSS
                     GFRETGPEGRALEERVLAEAARFEDLRILGPNCLGLIVPGRRLNASFAADAPRPGEIA
                     FLSQSGALGTSVLDWAIREKIGFSAFVSLGNSADVDFADLIDYFGEDEKTRSIILYIE
                     SLKNARRFMTAARAFARSKPIIVYKAGRFPESSEAAASHTGALASEDAVYDAAFQRAG
                     LARVFDIGEIFDVAELLGRRQIPKGDRLGIVTNGGGPGVMAVDALIAAGGGLAVFSDE
                     TKAGLDETLPPAGSHDNPVDILGDAKSKLAAKATARVLADPGVDAVLVIITPQAMTNP
                     SAIAREVGALAAGSTKPILAAWLGGESVAEGLRILTDAGIPAYDTPERAVRAFMTLVS
                     YARNLGILYETPKEVPVEFSADRKTRRERVRAFFESGTEMLGEEASKSVLEAYGIPAS
                     RPAPAASAEEAVRQAERMGYPVVLKIQSPDISHKTDVGGVVLDLGDAALVRAAFGRVT
                     AAAAAARPSARLEGVTVQPMIRAKEAVELILGAKKDSTFGSVMMAGLGGTTAELFGDR
                     ALGFPPLNERLARRMLESLKVWPLLRGYRGRPALAVDRLIETMIRLSYLVADHPEIKE
                     LDINPLLVSKDAVVALDARIAVDRGGGGASADPFAHLALRPYPEHYEKDVLLEDGSTV
                     RLRPIKPEDEPLWLALLSGCSKESIYSRFRYFFHWESHDVAARYCYIDYDRELAIVAE
                     EGEGPARRLTGVGRLVADPMKTSAEYAVLVGDAWQDKGLGGVLTDHCLDIARDWGVRK
                     ITAVTTTDNPRMIAVFRKRGFSIRVDPDGSTVDAAKELE"
     gene            complement(4501..5148)
                     /locus_tag="PKV64_10655"
     CDS             complement(4501..5148)
                     /locus_tag="PKV64_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HPN17574.1"
                     /translation="MFEAWLIIGPTGSGKTPLGEALELAGWNGRRCLHFDLGEELRRI
                     AAGRDVPPGLSSGEREVIGRVLRKGELFEDRQAPLVRKIIDDFLRRRGAREADLLLLN
                     GWPRHPGQARDLEAIARVRRIVFLNAPAAVLRERIRTDAGGDRAGRRDDSAGEVEAKL
                     ALFEERTRPLLAHYAAAGVPIVPLDAGPTDSAEDLLRTLRRAGEREGPGPRNGPE"
     gene            complement(5171..>5757)
                     /locus_tag="PKV64_10660"
     CDS             complement(5171..>5757)
                     /locus_tag="PKV64_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HPN17575.1"
                     /translation="YNAFCTFTVDNQWYAYLFPHEFGHSFAGLADEYYTSSVAYNEFY
                     PAGIEPREPNITALLDPAGLKWKDLLTPDIAVPTPWEKEEFDRSDTAYQKIRAEINDK
                     IARMKRAGAPAGEIAKMEEESERMSKEQAEWVDRFLAASKFAGKTGVFEGAGYAAEGL
                     YRPAVDCLMFTKGAKPLCPVCERAVRRMIEFYAE"
BASE COUNT          970 a         1911 c         1937 g          939 t
ORIGIN      
        1 gctccgccga atgccacgtc atccggactc cggaaagcgg cacctaccag tacgccgtct
       61 gcgtcgagga cgaggattac ggctggggga cggtcaagta ctccaaggga ggcaccgcct
      121 acgcggctta tcccatcgtc cggatctaca agggcaacac cctgatcaag gtcatcaatt
      181 cctcggccgc gacccgggtc ggaacgggaa cgctctactg gagcgtcttc aacctcaacg
      241 ccgcgaccgg ggccgtcacg gtgatcaaca agatcacgga cacgaagccg ttctacttct
      301 gatttgaggc cggccgcggg ccggtcgaaa tcgcgcgcgg ggccgcccgc ctgagggcgg
      361 cccctttttc atggggcgga aatctccgcg gtcagaagaa ataatagacc gcgccgagaa
      421 ggcggagatt gccgacccaa gcggccccgt tcacccggcc gtccgggccg gtttttccat
      481 aggcggcggc cgtatattcc gcctcgacgg ccagccggac cttgccggca ttgtagaccg
      541 cccgggggga aatccggaaa acggcgtcga tatcggcccc cctctcgtag agcggcccgg
      601 agacatttct tccgaagccg aaattctttg tataaccggc gaacaggccc gcctggaagg
      661 ccgccccgtt cgtctggatt tcgccccaca gggccagatt ctccgtcggc gcgtaggacg
      721 cttcagcccg gacggcgtcc gtgatttcag ccgcggcgta gcctcccatc atcgtcaggt
      781 gatgaaggtt ctgcccgtag acggcctcgg ccttgaccgt gaagcccggc tttttcagct
      841 tcccgaacgc catcccggcc cagtcgttca ggccggcgtc cgtccggtat ccgagcgtcg
      901 tggccaggcg ggggacgatc cgcttgtagt cgccgccgat cccgaacacg ctctcgcagc
      961 ccgcctcgca tgtccgccgg tactggagcg tcaggttgac ctcggggacg gccgcgtttc
     1021 gggcataggc cgtcgacgcg ccctcgggtc cggtcgaggc gaagtcgcgc tgggccaggg
     1081 cggcggccga gacgctccac cgttcgccga gcttccgggt gtacctgatc tggggcgagc
     1141 ggttgaacgg ctgaaaaggg gcgccggtgt tgaaggaaac gacgtcgggg aagctgtccg
     1201 tgatgaacat cggatgccag tactggccga tcaggagctc ggccttgggc caggcgagct
     1261 tgaggaagcc gtggcggagg cggaagccgt tgagatcgcc gtcggatgtc ccgaaaaact
     1321 cggcctcgat cagtcccgag gttttcgccc cgaaggcgtc gggcccggtg atccggccga
     1381 gaagccgcgt ctggaccgag agcatgtgga agctcgaggc cgcgttgacg tcccgcccgt
     1441 ccgggccggg ggcctcccct ttcggataaa ggagataatg cccttcccgg atggcgacgg
     1501 tctggcggga atcataaatc aagtcggtct tgaggaagcc ggagaaggat atcccgaagg
     1561 ccggggtttt cgcctcggtc tgggcccaca agccggccgc cccggccaag acggccgccg
     1621 ccaaaaatct tacgccgctg tttcttttca tggtcgtctc ctcactccag ttccttggcc
     1681 gcgtcgaccg tggatccatc cgggtcgacc cggatggaaa aaccgcgctt gcggaagacg
     1741 gcgatcatcc gggggttgtc ggtcgtggtc acggccgtga tcttccgcac gccccagtcc
     1801 cgggcgatat ccaggcaatg atcggtcaac acgccgccca aacccttgtc ctgccaggcg
     1861 tcgccgacca ggacggcgta ctcggccgac gttttcatcg ggtcggcgac cagccggccg
     1921 acgcccgtca agcgccgggc ggggccctcc ccctcctcgg cgacgatcgc cagctcccgg
     1981 tcgtaatcga tgtagcaata gcgggcggcc acgtcgtgcg attcccagtg gaaaaagtaa
     2041 cggaaccggg aatagatcga ctccttcgag catccggaaa ggagggccag ccacaacggc
     2101 tcgtcctcgg gtttgatcgg ccgaagacgg accgtcgagc catcctccag gaggacgtct
     2161 ttctcgtaat gctcgggata cggccgcagg gccaggtggg cgaagggatc ggccgaggcg
     2221 ccgccccccc cgcggtcgac ggcgatccgg gcgtccaggg cgacaaccgc gtctttcgag
     2281 acaagcaggg gattgatgtc cagctccttg atttcgggat gatcggcgac caggtaggac
     2341 aagcggatca tcgtttcgat cagccggtcg acggccaggg ccggccgccc gcggtatccg
     2401 cgaagaagcg gccagacctt cagcgattcg agcatccggc gggccagccg ctcgttgagc
     2461 ggcgggaaac ccagggcccg gtcgccgaac agctcggccg tcgtcccacc cagcccggcc
     2521 atcatgaccg agccgaacgt ggaatccttt ttcgccccga gaatcagctc gacggcctcc
     2581 ttggcccgga tcatcggctg gaccgtgacc ccctcgagcc gggccgaggg ccgggcggcc
     2641 gcggccgcgg ccgtcacccg gccgaaggcg gcccggacca gggccgcgtc gcccaagtcc
     2701 aagacgaccc cgccgacgtc ggtcttgtgg gaaatgtccg gcgactggat tttcaagacg
     2761 acgggatacc ccatccgctc cgcctgccgg acggcctcct cggccgatgc ggccggcgcc
     2821 ggacggctgg cgggaattcc gtaggcctcg aggaccgatt tggaggcctc ctcgcccagc
     2881 atttccgttc cggattcaaa gaaggctcga acgcgttccc tccgggtctt gcggtcggcg
     2941 gaaaactcga ccgggacttc cttgggcgtt tcataaagaa tcccgaggtt gcgggcgtag
     3001 gagacgaggg tcatgaaagc tcggacggcc cgttccgggg tgtcataggc cgggatgccg
     3061 gcgtccgtga ggatccgaag gccttcggcc acgctctctc ctcccagcca agcagccagg
     3121 atgggcttgg tcgagccggc ggccagggcc ccgacttccc gggcgatcgc cgacgggttg
     3181 gtcatggcct gaggcgtgat gatcaccagg acggcgtcga cgccgggatc ggccagcacc
     3241 cgggcggtcg ccttggccgc cagcttggac ttggcgtccc ccaggatgtc gaccggattg
     3301 tcgtgcgatc ccgccggggg cagggtttcg tcgaggccgg ccttggtttc gtcgctgaag
     3361 acggccaggc cgccgccggc ggcgatcagg gcgtcgacgg ccatgacgcc cggcccgccg
     3421 ccgttggtca cgatgcccag ccggtcgccc ttggggatct gccgccggcc caaaagctcg
     3481 gccacgtcga agatctcgcc gatgtcgaac acccgggcca ggccggcccg ctgaaaggcc
     3541 gcgtcgtaga ccgcatcctc cgaggccagg gccccggtat gggaagcggc cgcttcggac
     3601 gattcgggaa aacggccggc cttgtagacg atgatcggct tggaccgggc gaaggcccgg
     3661 gccgcggtca tgaaccgccg ggcgtttttg agcgattcga tatataagat gatgctccgg
     3721 gttttctcgt cctcgccgaa atagtcgatc aaatcggcga aatccacgtc ggccgagttg
     3781 ccgagggaga cgaacgccga aaatccgatc ttttcccgga tggcccaatc gaggaccgac
     3841 gtgccgagcg cccccgactg ggaaaggaag gcgatctccc ccggccgggg cgcatcggcc
     3901 gcgaagctgg cgtttagcct ccggcccgga acgatcaggc cgaggcagtt gggcccgaga
     3961 atcctcaaat cctcgaaccg ggcggcctcg gccaacaccc tctcctcgag agcccgcccc
     4021 tccggccccg tttcccggaa acccgaggag acgatgacga gggaccggag gccggcttcg
     4081 ccgcattccc ggacgaggcc ggggacctcg gcggccggac cgcagagaat gccgaggtcg
     4141 ggcgtgcggg gaaggcttcg gaggccgggg tagcagggaa tccccataac cgcttccacg
     4201 gccggattca cgggataaac gacgcccagg aatccgctgc cgacgagatt gcccaggatt
     4261 tttccgccga cgctgttcgg attgacggtg acgccgataa gggcgattcg ctgcgggttg
     4321 aaaaatccgt ccagttgatg gatgttcata aagacgactc cggggggaat gagcgtgtat
     4381 tttctcccaa agcgtcccgc gtttcaatcc cttgccggag cgttgcggcc gagcggccgc
     4441 cggagaacgc gctgagtaaa ttgagaaaac tggccggccg gggcgtccgc cccgcggccg
     4501 tcactccggg ccgttccggg ggcccggtcc ctcccgctcc cctgcgcggc gaagggtgcg
     4561 gaggagatcc tcggccgaat ccgtcgggcc ggcgtccagc ggaacgatcg gaacgccggc
     4621 ggccgcgtag tgggccagga gcggcctggt ccgctcctcg aacaaggcca gcttggcctc
     4681 gacctccccg gccgaatcgt cccggcggcc cgcccggtct ccgccggcgt cggtccggat
     4741 cctttcccgg agaacggcgg ccggagcgtt cagaaagacg atccgccgga cgcgggcgat
     4801 cgcctccagg tcccgggcct ggccggggtg gcgcggccag ccgttcagga ggagaaggtc
     4861 agcttcccgg gcgccgcggc ggcgcaggaa gtcatcgatg attttccgga cgaggggagc
     4921 ttggcggtcc tcgaaaagct cccccttccg gagaacgcgg ccgatgacct cccgttcccc
     4981 ggacgataag cccgggggaa cgtcccggcc ggcggcgatc cggcgcagct cctcccccag
     5041 gtcgaaatga agacaccggc gcccgttcca cccggcgagc tccagggctt cccccagggg
     5101 cgtcttgccg gatcccgtcg gaccgatgat cagccaggcc tcgaacatgc ggacggccgc
     5161 cgacggccgt tcactccgcg tagaattcga tcatccgccg gacggcccgc tcgcacaccg
     5221 ggcagagggg cttggccccc ttggtgaaca tgaggcaatc cacggccggc cggtagagcc
     5281 cctcggccgc gtagccggcg ccttcgaaga cgcccgtttt cccggcgaac ttgctcgcgg
     5341 ccaggaaccg gtccacccac tcggcctgtt ctttcgacat ccgctcggat tcctcttcca
     5401 tcttcgcgat ctcgccggcc ggggcgccgg cccgcttcat ccgggcgatc ttgtcgttga
     5461 tttcagcgcg gatcttctgg taggccgtgt cgctccggtc gaactcttct ttttcccagg
     5521 gcgtcgggac ggcgatgtcc ggggtgagga gatccttcca tttcaggccg gccgggtcga
     5581 gcagggcggt gatgttaggc tcgcggggct cgattccggc cgggtagaat tcattgtacg
     5641 ccaccgagga ggtgtagtac tcatcggcca ggccggcgaa ggaatggccg aattcatggg
     5701 gaaagaggta agcgtaccat tggttgtcga cggtgaaagt gcagaaagcg ttgtaga
//
LOCUS       DAOOXH010000124         5693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_16369_length_5693_cov_3.630897, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000124 DAOOXH010000000
VERSION     DAOOXH010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16369_length_5693_cov_3.630897"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /locus_tag="PKV64_10665"
     CDS             complement(<1..1441)
                     /locus_tag="PKV64_10665"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPN17576.1"
                     /translation="MQRNRKHMWAALLAVVLLIVGISLPARGQSILTGKISGTITDDA
                     GEALPGVNVEVTSPALISGKRATVSTARGSFVFLDLPPGEYKITASLENFKTIVQEGI
                     MVSAGSSLVVNLALPTGNINETVEVVGAAPIVDSKSSTIDSKIQAEMLTKLPTSRDAF
                     YDLSLTTPGMFDHGSSGGWMPSPTAYGGASNENVFLVNGVNATNPRGASFGTLVRVNY
                     NAVEEVRVVALGSKAEYGSYSGAAIDVLTKSGSNSFHGAAAVYFEPVKWRTSNQPATN
                     EKYGTDWIYVAPDVTLYGGPPKSDYELNLTFGGPIIKDKIWFFGGIDYLYNAGKRPRW
                     DLLAESWGRYADMKISAEPFTAHRAWISYHYESNDYTGGSWGSQPEWDTSASYGVASV
                     DHSVSAQWQWLPSSTTILTAKYLGFWTNDTPHVPDDAPDHPCYINWWKWAQYGINGAF
                     PYIEGWHSNRQTIQADVSHYAEDFLGEHDI"
     gene            complement(1584..2729)
                     /locus_tag="PKV64_10670"
     CDS             complement(1584..2729)
                     /locus_tag="PKV64_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HPN17577.1"
                     /translation="MILKTVYKNDRISRAAIARVTGLTRATVSGAVADLENDGMIAEV
                     GVGPSKSGKPPILLKFVDEARCLIGLDLAENEFRGAIVNLRGEIKHRLNWPINGGDVS
                     GTLASIDALIDRLRRLSTSPLLGIGIGAPGLIDSQHGVVRYAAKLDWREIRLGEMLAR
                     KHGLPVHIANDCQAAALAEFTFGNGNGYEPNLIVIKASQGIGAGIVLNRQLFCGDRFG
                     AGELGHLVINEGGDPCRCGHRGCLETVVGSESILRAARGIAAGDPGSLLHRFAGAPER
                     IDIDAVARALEAGDGTLRSMVAEAGRILGIALANLVGILNVHRLILAGSLTRLGPALL
                     DPVRREIGRRSLSALADETVVAVSGLGEDIVILGAAALLLNNELKLP"
     gene            complement(2876..3841)
                     /locus_tag="PKV64_10675"
     CDS             complement(2876..3841)
                     /locus_tag="PKV64_10675"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN17578.1"
                     /translation="MNKRAFCFGAVLAAAVLAWGTVSAFGQYREYYFFGRVVDTDTKP
                     VAGVEILLRDVATSRGYTIKTNDRGEFKFAGLPHGIYTVTFKKEGFAVKEDEWRFPNP
                     QDTMQKVEIPDVTLVSQELVDKAARLKIDEARIQEAVEKIRNKDYDGAITLLRDFLEK
                     NPDEVNALYFLGLSYARKKMFAEAVETLGRVAEKTPDFPPVFFELGLCYKQLDEPEKA
                     IAAYQRNQELDPTNADSAYNSGLILFRLGRVPEAQKAFEDALALKPDDPEILEMVSRC
                     YINGGDFVKAVEYLEKAKAKTTDSDKIKFIDDLIHTLKEKIKGGC"
     gene            complement(3834..>5693)
                     /locus_tag="PKV64_10680"
     CDS             complement(3834..>5693)
                     /locus_tag="PKV64_10680"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPN17579.1"
                     /translation="FDPARYDRILLENEKRAEEVLADARQWLAGIGQKRFFAWIHLYD
                     PHTPYAPPEPYYSRFAPGGPAGLYDGEIAFMDEQIGRLTSWLERTGLDRRTILLFVGD
                     HGEGLGSHGESSHGYFIYDYAQRVPLIVVTPLAKGRGVRVTSQVSTVDVFATILELAG
                     MSVPAGRQGRSLVPLMFGRKEAKEESAYGESMAPNIQFGWAPLKALRTVRYKFIDAPR
                     LELYDLEKDPAESVNIAGRNPALVRDFKARLDRFVEESGRGAPKLQAADLDKETMARL
                     SALGYIGAPVASKKSGGGAPADPKDKLLVYESVQRAGEMIMGERYDEAAVLLEATLRS
                     DPDIAQALLLLATCRSETGKMEEAKEALLHILGSEPENIQALISLANILMSEGRMEEV
                     AAVCKQTLAVDEKSVQAHMLLGEVYMAEEKHAEALPFLEKAFEIQPKLTQNGLNLAAC
                     LIGLDQFERAEPLLKSVIAENPRFPFANFNLGLLYEGLGRWEDARRAYTEEIASYPQE
                     YRARFNRGKIFFRLGERQAYLDEMREVVAIAPRQAEGYLFLARGLLSEPGSIDEAAGL
                     VEKGLSLAATDELKAFGYFLQADIFNRKGQPEKMRTALEKANAFKSKEESAHE"
BASE COUNT          994 a         1756 c         1784 g         1159 t
ORIGIN      
        1 tgatgtcgtg ctcgcccaga aagtcctcgg cgtagtgcga cacgtcggcc tggatcgtct
       61 ggcggttcga atgccagccc tcgatgtagg ggaaagcccc gttgatgccg tactgggccc
      121 acttccacca gttgatgtag cagggatggt ccggggcgtc gtcgggcacg tggggcgtgt
      181 cgttcgtcca gaatcccagg tacttggccg tcaggatggt cgtgctggac gggagccatt
      241 gccactgggc ggacacggaa tggtcgacgc tggccacgcc gtaggacgcg gatgtatccc
      301 attccggctg gctgccccag cttccgccgg tgtagtcatt gctctcgtaa tggtaggaga
      361 tccaggcccg gtgagcggtg aagggctcgg ccgagatctt catgtcggcg tagcggcccc
      421 agctttcggc caaaagatcc caacgggggc gcttcccggc gttgtagaga taatcgattc
      481 cgccgaagaa ccagatcttg tccttgatga tcgggccgcc gaacgtcagg ttcagctcgt
      541 aatcgctctt cggcggtccg ccgtaaaggg tgacgtcggg ggcgacgtag atccagtcgg
      601 tgccgtactt ctcgttcgtc gccggctggt tgctcgtccg ccacttgacg ggttcgaaat
      661 agacggccgc cgctccgtgg aacgagttgc ttccggactt ggtcaggacg tcgatcgccg
      721 cgcccgagta ggatccgtat tcggccttgg atcccagggc cacgacccgg acctcctcga
      781 cggcgttgta gttgacccgg accagcgttc cgaaggaggc gccgcgcgga ttggtggcgt
      841 tgacgccgtt gacgagaaag acgttctcgt tggaggcgcc gccgtaggcc gtcgggctcg
      901 gcatccagcc gcccgagctg ccgtggtcga acatgccggg cgtcgtcagg gacaggtcat
      961 agaacgcatc ccggctcgtc ggcagcttgg taagcatctc cgcttggatc ttggagtcga
     1021 tcgtcgacga tttcgagtcg acgatgggcg cggcgcccac cacttcaacc gtctcgttga
     1081 tgttgcccgt cgggagagcc aggttgacga ccagactgct ccccgccgag accatgatgc
     1141 cttcctgaac gatggttttg aaattttcca gggacgccgt gatcttgtat tcgccgggcg
     1201 gcaaatccaa gaaaacaaac gatccccggg cggtggagac ggtcgcccgc ttccccgaga
     1261 tgagggccgg gctggtcacc tccacgttga cgccgggcaa cgcttcaccg gcgtcgtcgg
     1321 taatcgttcc cgagattttt ccggtgagaa tcgattgacc gcgcgccggc aggctgatcc
     1381 caacgatcaa caggacgacg gccagcaggg ccgcccacat gtgttttcga ttcctctgca
     1441 tctcattctc ctctttgtgg gtttgccttc cttcctgtct cttccggttc cctcggaaga
     1501 gaccctcccc aggtgatctt tgtacggcgt tcgttatatt ctcacccccc tccggacgcg
     1561 ggcctgctca ttcttggctt gtttcatggc agcttcagct cattgttgag gagcagggcg
     1621 gcggcgccta agatgacgat gtcttcccca agcccggaga ccgcgacgac ggtttcgtcg
     1681 gccaaggccg agagcgaccg ccgtccgatc tcccgccgaa ccggatcgag caaggccggc
     1741 ccgagtcgag tcaggctgcc ggccaggatg aggcgatgga catttaaaat ccccacgagg
     1801 ttggccaggg cgatgccgag gatgcggccc gcctccgcga ccatggatcg aagcgtcccg
     1861 tcccccgcct ccaaggcccg ggcgacggcg tcgatatcga tccgctccgg cgcgccggcg
     1921 aaccggtgaa gaagggagcc gggatcgccg gccgcgatcc cccgggcggc ccggagaatc
     1981 gactcgcttc cgacgaccgt ttccaggcag cctcggtggc cgcagcggca ggggtcgccc
     2041 ccttcattga tgaccaaatg gccaagctcc cccgcgccga accggtcgcc gcagaagagc
     2101 tgccggttga ggacgatccc cgccccgatg ccctggctgg ccttgatgac gatcaggttc
     2161 ggctcgtaac cgttgccgtt gccgaacgtg aattcggcca aggccgccgc ctggcaatcg
     2221 ttggcgatat gaacgggcag gccgtgtttc cgagcgagca tctcccccaa gcggatttcg
     2281 cgccaatcga gcttggccgc gtaccgaacg acgccgtgtt gggaatcgat caaacccggg
     2341 gcgccgatcc cgatgccgag aagagggctg gtcgaaagcc ggcggagacg atcgatcagg
     2401 gcgtcgatcg aagccagcgt gccggaaacg tcgccgccgt tgatcggcca gttcaggcgg
     2461 tgtttgattt ccccccggag attgacgatc gccccccgga attcgttctc cgccaggtcg
     2521 aggccgatca ggcagcgggc ctcgtcgacg aacttcaaga gaatcggggg tttgccgctt
     2581 ttggacggac cgacgccgac ttcggcgatc atgccgtcgt tttcgagatc ggcgacggcg
     2641 ccggaaaccg tcgcccgggt caatcccgtc acccgggcga tcgcggcccg gctgatccgg
     2701 tcgtttttat agacagtctt cagaatcaga agacgattgt gactcttcgt gtgttgcgaa
     2761 ttaacttttt tgattggatt tcccgttcca acgcatttgt aagtttattg aacttacaaa
     2821 ttcaagatta cttaaaaaaa atataatgtc aagaggtaaa aatccagggc ggacgtcagc
     2881 atccgccctt gattttttcc ttgagagtgt ggatgagatc atcgatgaat ttgattttat
     2941 ccgaatccgt ggtcttggct ttcgcctttt cgagatactc cacggccttg acgaaatcgc
     3001 cgccgttgat atagcaccgg ctgaccattt ccaggatttc aggatcgtcg ggtttgaggg
     3061 cgagagcatc ctcgaaggct ttctgggcct cggggacgcg ccccaagcgg aaaagaatga
     3121 ggccggaatt ataggcgctg tcggcgttgg tcggatcgag ctcctggttt ctttgatacg
     3181 cggcgatcgc tttttccggc tcgtccagtt gcttataaca gaggccgagc tcgaagaaaa
     3241 ccggcggaaa gtcgggggtc ttttcagcga cccggccgag cgtctcgacc gcctcggcga
     3301 acatcttttt ccgggcgtag ctcagcccga ggaaatagag ggcgttgacc tcgtccggat
     3361 tcttctccag gaaatcccga agcaaggtga tcgccccgtc gtaatccttg ttgcggatct
     3421 tctcgactgc ttcctggatc cgggcctcgt cgatcttgag cctggcggcc ttgtcgacga
     3481 gctcctggga gaccagcgtc acatccggaa tctcaacctt ctgcatcgtg tcctggggat
     3541 tcgggaagcg ccactcgtct tccttgaccg cgaatccttc cttcttgaag gtcacggtgt
     3601 agatgccgtg gggcaggccg gcgaatttga attccccccg gtcgttcgtc ttgatggtgt
     3661 atccccggct cgtcgcgacg tcccggagaa ggatttcgac cccggccacc ggcttcgtgt
     3721 ccgtgtcgac gactcttcca aaaaagtaat actcccggta ctggccgaag gccgagacgg
     3781 ttccccaggc gagaacggcg gccgccagaa ccgctccgaa acaaaacgct cgcttattca
     3841 tgggctgact cctcctttga cttgaacgca ttggcttttt caagggccgt ccgcatcttt
     3901 tcgggctgtc ctttccggtt gaagatatcg gcttgcagga aatatccgaa ggccttgagc
     3961 tcgtccgtcg cggccaggga aagccccttt tccacaaggc cggcggcctc gtcgatcgac
     4021 cccggctcgc ttaaaagccc ccgggccagg aaaaggtacc cctcggcctg gcgcggggca
     4081 atagccacga cctcccgcat ctcgtccaaa taggcctgtc tttcccccag ccggaagaag
     4141 atcttccccc ggttgaagcg ggcccggtac tcttgggggt aggaggcgat ctcctcggtg
     4201 tacgcccttc gggcgtcctc ccagcggccc agtccctcgt aaagaagccc caggttgaaa
     4261 ttcgcgaagg gaaaccgcgg attctccgcg atcaccgact tgagcaaggg ctccgcccgt
     4321 tcgaattgat cgagcccgat caggcaggcc gcgaggttga ggccgttttg agtcagcttg
     4381 ggctggatct cgaaggcttt ttccaggaac ggcagggcct cggcgtgctt ctcctccgcc
     4441 atgtagactt cccccaggag catgtgggcc tgaacgcttt tctcgtcgac cgccagggtt
     4501 tgtttgcaga cggccgcgac ttcctccatc cggccctcgc tcatgaggat gttggccagc
     4561 gagatcaggg cttggatgtt ttcgggttcg gagccgagga tgtggagaag agcctccttg
     4621 gcctcttcca tcttgcccgt ctccgaccgg caggtggcca ggagaagaag ggcctgggcg
     4681 atatccggat cgctccgaag cgtcgcctcg agaagcacgg ccgcctcgtc gtaccgctcg
     4741 cccatgatca tctctccggc ccgctggacg gattcgtaaa cgaggagttt gtccttgggg
     4801 tcggccgggg ctccgcctcc ggattttttc gaggcgaccg gagccccgat atagccgagg
     4861 gcggacagcc gggccatcgt ctccttgtcc aggtcggcgg cctgaagctt cggcgcgccg
     4921 cggcccgatt cctcgacgaa ccggtccagc cgggccttga aatcgcggac gagggccgga
     4981 ttccggccgg cgatattcac cgattcggcc gggtccttct caaggtcgta aagctccagg
     5041 cgcggagcat cgatgaactt gtaccggacg gtccgcagag ccttaagcgg cgcccagccg
     5101 aattggatgt tcggcgccat ggattcgccg taggcggact cttccttcgc ctctttccgg
     5161 ccgaacatca gcgggaccaa ggaccggcct tggcggcccg ccggaacgga catcccggcc
     5221 agctccagga tcgtggcgaa gacgtcgacg gtgctgacct gggacgtcac ccggacgccg
     5281 cggcctttgg ccaagggcgt cacgacgatc aacgggacgc gctgggcgta gtcgtaaatg
     5341 aaatacccgt ggctgctttc gccgtggctg cccaggccct cgccgtggtc gccgacaaag
     5401 agaagaatcg tccgccggtc caggccggtg cgctccagcc aggacgtcag gcgcccgatc
     5461 tgctcgtcca tgaaggcgat ctcgccgtcg tagaggccgg ccggaccgcc gggcgcgaac
     5521 cggctgtaat agggttcggg aggggcataa ggcgtgtggg gatcgtagag gtggatccag
     5581 gcgaagaagc gcttctggcc gattcccgca agccactggc gggcgtcggc gagcacctcc
     5641 tcggcgcgct tttcgttctc cagcaggatg cggtcgtagc gggccgggtc gaa
//
LOCUS       DAOOXH010000125         5647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_16568_length_5647_cov_7.358011, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000125 DAOOXH010000000
VERSION     DAOOXH010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16568_length_5647_cov_7.358011"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /locus_tag="PKV64_10685"
     CDS             complement(<1..1480)
                     /locus_tag="PKV64_10685"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPN17580.1"
                     /translation="MNLTDLLQGISAVSLEGDPKLEISGLAYASADVRPGFLFAAWKG
                     TLRDGFEFVDEAVGRGAAAVLSDRPRPGGRNVAWIQVFNPREALALAAANFYGRPAAR
                     MKIVGITGTKGKTTISYILESILRQAGYAVGVIGTISHRGPGFELQASRTTPESVDLQ
                     RMLKEMADRGVTHCLMEVSSHSLELHRVSGIGFDVAVFTNLSGEHLDFHRTMEDYFEA
                     KKKLFFLNAKKRTAVINLDDSWSRRLIAELPMTTVTFGLEPEALVRAERPKFSGAGIE
                     AVISYPGGRIPIVSPLAGRHNLSNLLAAFAVSMALGVPVPLIKEGIAGLAQIPGRFEK
                     IENSLGFHVVVDYAHTDASLQSLLETVRDLKPARILLVFGAGGDRDRAKRPRMGAVAA
                     RYADWTFLTSDNPRSEDPAAIIAEIEAGFTAAGGAKYTIIPDRREAITRALAEAGEGD
                     FVVLAGKGHETTQTSGTNVRPFSDAGVAREILAMSERDRYFPG"
     gene            complement(1477..3273)
                     /locus_tag="PKV64_10690"
     CDS             complement(1477..3273)
                     /locus_tag="PKV64_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPN17581.1"
                     /translation="MKAAALLPGEEIARRTARIRTRVVLAVMILWAGGIVVRLVQLQV
                     FFHGRAKEQIAYQNQAKVKIFPERGMIRDRRGMILAQMVSLRSVFYAPPAKETRAARR
                     TVIRKLASCLDLDEKAVAGIEAAADQRKPFIWIKRKIGLETAAAVDALRLPGVFTQEE
                     PQRVYPQGALASHVLGGVNIDNDGLAGVEILFNDTLRGKPGEQIIYRDTYKRPYRSEQ
                     IIAPESGRDIDLTIDAVIQYIAQREIEKAVAENNAAWGAVIVSDPATGEILALANAPS
                     YDPNLYGKADPAARMDWAYSRRIDPGSTFKIVTASAALENRKVATYETFDCRASAIDV
                     AGGPIRDHKPFGILSFSGIIAESSNIGTIQIGRRVGAELMYRTIKDFGFGERTGIELP
                     TESAGLVHPPDRWSRRSIDSVSVGYEVSVTPLQLLQAINIVANRGVRLAPRIIKAVDG
                     RPRPPQADEAPPIEVISPQTAEKLIEILERAVLGGTGKAAAVPGYTVAGKTGTSQIYD
                     PIEGKYSLSRHTASFIGFVPSNKPALSMAVVLFEPRNNAYYGGQVAAPVFRAIARPVL
                     RYLHVFPDTAPATVHASAASAAPPAFRQEAEE"
     gene            complement(3270..3560)
                     /locus_tag="PKV64_10695"
     CDS             complement(3270..3560)
                     /locus_tag="PKV64_10695"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HPN17582.1"
                     /translation="MVHKKFTRRQIVLTAGTALVVVFILAFYLWQVAETVRLGYEANE
                     AENEKKALEKEVLRLQADKAALLSLERVERTAREKLGLTDPREDQIIYEDFR"
     gene            complement(3550..4470)
                     /gene="rsmH"
                     /locus_tag="PKV64_10700"
     CDS             complement(3550..4470)
                     /gene="rsmH"
                     /locus_tag="PKV64_10700"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPN17583.1"
                     /translation="MTGRDHIPVLLDESLTLLDAGRSGVYIDGTLGLAGHSREILKRN
                     PGARLVGFDRDEAALAEAQRRLSPYQNRIALYHADFRRLLEVEIDWASVRGVFLDLGM
                     SSFQLDDPARGFSHQLDGPLDMRMDRRQHLDAARVIDKYPEARLADIFRNYGELRPAA
                     KLARMIASRRRMTKIESTVQLRTIVEEVCRWIPQKGKTHPAAKVFQALRIEVNEELAG
                     LREFLESVIARLPEDGRLAVISFHSLEDRIVKHTFLQAARPDQETGRRPAQVELLTKH
                     PVVPGEAEISRNSRSRSAKLRAVRKQAHGS"
     gene            complement(4467..4934)
                     /locus_tag="PKV64_10705"
     CDS             complement(4467..4934)
                     /locus_tag="PKV64_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17584.1"
                     /translation="MGNLLIGSYRAKLDKSGRLKVPEKFRTALEERYGLDFFVTSLES
                     STIKIFPLPVWLAMTGTTESGNLYLDPDVEDYMRRANTFGVQCELDAKGRVLISPDLR
                     AMAGLDAEVVVIGLNNHLEVWDTRRLDEKLQKKPLTRDDFKKIAEIMTGRKGP"
BASE COUNT          950 a         1660 c         1980 g         1057 t
ORIGIN      
        1 ggcccggaaa ataccggtcg cgctcgctca tggccaggat ttcccgggcg acgccggcgt
       61 cgctgaacgg cctgacgttt gtcccggatg tctgggtcgt ttcgtgtccc ttgccggcca
      121 ggacgacgaa gtcgccttcg ccggcttcgg ccagggcccg ggtgatcgcc tcgcggcggt
      181 cggggatgat ggtgtatttc gcgccgccgg cggccgtgaa gcccgcctcg atctcggcga
      241 tgatcgccgc cggatcctcg ctccggggat tgtccgaggt gaggaaggtc caatcggcgt
      301 accgggccgc gacggccccc atccgggggc gcttggcccg gtcgcggtcg ccgcccgccc
      361 cgaacacgag gagaatccgg gccggcttga gatcccggac ggtctcgagc aggctctgga
      421 gggaagcatc ggtgtgggca tagtcgacga cgacgtggaa accgagggaa ttttcgattt
      481 tctcgaagcg tccgggaatc tgggcgagtc cggcgattcc ctccttgatc aggggcacgg
      541 ggacgccgag ggccatggag acggcgaacg cggccaggag gttggataaa ttgtgccgtc
      601 cggccagggg ggaaacgatg ggaatccggc cgccgggata ggaaatgacg gcctcgatcc
      661 ccgccccgct gaatttcggt cgttcggccc ggaccagggc ttccggctcg agaccgaagg
      721 tgacggtggt catcggaagc tcggcgatga ggcgccggct ccaggaatcg tccaggttga
      781 tgaccgccgt tctcttcttg gcgttgagga aaaaaagctt tttcttggcc tcgaaatagt
      841 cttccatcgt ccggtgaaag tcgaggtgtt ctcccgagag gttggtgaac acggccacgt
      901 cgaatccgat cccggagacc cggtgaagct caagcgaatg ggacgagact tccatgaggc
      961 agtgggtgac gccccggtcg gccatttcct tgagcatgcg ctggaggtcg accgattccg
     1021 gggtggtccg ggaggcctgg agctcgaagc cggggccgcg gtgggaaatc gtgccgatga
     1081 ctccgacggc gtatccggcc tgcctgagga tggattcgag gatgtaggaa atcgtcgtct
     1141 tgcccttggt cccggtgatt ccgacgatct tcatccgggc ggccggccgg ccgtagaaat
     1201 tggccgcggc cagggccagg gcttcacgcg gattgaaaac ctggatccag gcgacgttcc
     1261 gtcctcccgg ccgggggcgg tcggagagaa cggcggccgc cccccgcccg acggcctcgt
     1321 cgacgaactc gaatccgtcg cggagagtgc ctttccaggc ggcgaaaaga aagccggggc
     1381 ggacgtcggc cgaggcgtag gccaagccgg agatctcgag cttcggatcg ccttcgagcg
     1441 acacggcgga aatcccttgg aggagatcgg tcagattcat tcctcggcct cctggcggaa
     1501 agcgggcggc gcggcggacg cggcggaggc gtgcaccgtc gcgggggccg tgtcgggaaa
     1561 gacatggaga taacggagga cgggccgggc gatggcccgg aaaacaggag cggccacctg
     1621 gccgccgtaa taggcgttgt tccggggctc aaagagaacg acggccatgg agagggccgg
     1681 cttgttcgag gggacgaagc cgatgaacga ggccgtgtgg cggctgagcg aatatttgcc
     1741 ctcgatcgga tcgtagattt gggaagtccc ggttttgccg gcgaccgtgt agccggggac
     1801 ggccgcggct tttcccgttc ccccaagcac cgccctctcc aggatttcga tgagcttctc
     1861 ggccgtctgg ggggagatca cctcgatcgg cggagcctcg tcggcctggg gcggccgggg
     1921 ccgcccgtcc accgccttga tgatccgcgg ggcgagccgg acgccccggt tggcgacgat
     1981 gttgattgcc tggagaagct ggaggggcgt gacggaaacc tcgtagccga cggagacgga
     2041 atcgatggac cggcgggacc agcggtccgg cggatggacg aggccggccg attcggtcgg
     2101 cagctcgatc cccgtgcgct ctccgaagcc gaaatccttg atggtcctat acatcagctc
     2161 ggcgccgacg cggcggccga tctggatggt tccgatgttg gaagattcgg cgatgatccc
     2221 ggagaaggaa aggatgccga acggcttgtg gtcgcggatg gggccgccgg ccacgtcgat
     2281 cgcgctcgcc cggcagtcga atgtctcgta ggtcgccacc ttgcggtttt ccagggcggc
     2341 cgaggcggtg acgatcttga aggtggagcc cgggtcgatc cgccggctgt aggcccagtc
     2401 catgcgcgcg gccggatcgg cctttccgta gagattcggg tcgtaggagg gggcgttggc
     2461 cagggccagg atctcgccgg tcgccggatc ggagacgatc acggcgcccc aagcggcgtt
     2521 gttctcggcc acggcctttt cgatctcgcg ctgggcgatg tactggatca ccgcgtcgat
     2581 cgtcaggtcg atgtcccggc cgctttcggg ggcgatgatc tgctcgctcc ggtacggccg
     2641 cttgtacgtg tcgcggtaga tgatctgctc gcccggcttg ccgcggagcg tgtcgttgaa
     2701 gaggatttcc accccggcca gcccgtcgtt gtcgatgttc actccgccca ggacgtggga
     2761 ggccagggcg ccctgggggt aaacccgctg cggctcctcc tgggtgaaga cgccgggaag
     2821 ccggagcgcg tcgacggcgg ccgcggtctc gaggccgatt ttgcgcttga tccagatgaa
     2881 gggctttctt tgatcggcgg ccgcctcgat ccccgcgacg gctttctcgt cgaggtcgag
     2941 acatgaggcg agtttccgga tgacggtccg ccgcgccgcc cgcgtctcct tggccggcgg
     3001 ggcgtagaag acggaccgga gggaaaccat ctgggccagg atcatccccc ggcggtcgcg
     3061 gatcatccct cgttcgggga atatcttcac cttggcttgg ttctggtaag cgatctgctc
     3121 cttggcccgg ccgtggaaga aaacctggag ctggacgagg cggacgacga ttcccccggc
     3181 ccagaggatc atgaccgcca ggacgacgcg ggtccggatg cgggccgtcc ggcgggcgat
     3241 ctcctcgccg ggaagtaagg ccgcggcttt catcgaaaat cctcgtagat gatttgatcc
     3301 tcgcgggggt cggtcaagcc gagcttctcg cgggccgtcc gctccacccg ctcgagcgaa
     3361 agaagagcgg ccttgtcggc ctggagccgg aggacttcct tttccagcgc ctttttctcg
     3421 ttttccgcct cgttggcctc gtatcccagg cggaccgtct cggcgacctg ccagagatag
     3481 aaggccagga tgaagaccac aaccagggcc gtcccggcgg tgaggacgat ctggcggcgg
     3541 gtgaacttct tatgaaccat gagcctgttt ccggacggcc cggagcttgg ccgagcggga
     3601 ccgggagtta cgggagattt ccgcttcgcc ggggacgacg ggatgcttgg tcaggagctc
     3661 gacttgggcg ggccgccggc ctgtttcctg gtcgggccgg gccgcctgga ggaaggtgtg
     3721 cttgacgatc cggtcctcga gcgagtggaa ggagatcacg gccaggcggc cgtcttcggg
     3781 aagacgggcg atgacgcttt cgagaaattc ccggaggccg gccagctctt cgttgacctc
     3841 gatccggagg gcctggaaga ccttggccgc gggatgggtc tttccctttt ggggaatcca
     3901 gcggcagacc tcctcgacga tggtccgcag ctggaccgtg ctttcgatct tcgtcatccg
     3961 ccggcgggag gcgatcatcc gggccagctt ggcggccggc cggagctcgc cgtaattgcg
     4021 gaagatgtcg gccaggcggg cttcgggata cttgtcgatg acccgggccg cgtccaagtg
     4081 ctggcgccgg tccatccgca tgtccagcgg gccgtccaac tggtggctga agccgcgggc
     4141 cggatcgtcg agctggaacg acgacatgcc caagtcgagg aaaacgccgc ggacggacgc
     4201 ccagtcgatc tccacttcga ggagccggcg gaagtcggcg tggtagagcg cgatccggtt
     4261 ttggtagggg ctgaggcgcc gctgggcttc ggccagggcg gcctcgtcgc ggtcgaatcc
     4321 gaccagccgg gcgccgggat tgcgcttcag gatctcgcgg gagtggccgg ccagcccgag
     4381 ggttccgtcg atatagacgc cggaccggcc cgcgtccagg agggtcaggg attcatcgag
     4441 caggaccggg atgtgatcac gcccggtcat ggccctttcc tgcccgtcat gatctcggcg
     4501 atcttcttga agtcgtcgcg ggtcagcggc ttcttctgga gcttctcgtc gagccgccgg
     4561 gtgtcccaga cctcgaggtg gttgttgagg ccgatgacga caacctcggc gtcgaggccg
     4621 gccatggccc gaaggtcggg gctgatcaaa acccggccct tggcgtccag ttcgcactgc
     4681 acgccgaagg tgttggcccg ccgcatgtaa tcctcaacgt cggggtccag gtagagattg
     4741 ccgctttccg tcgtcccggt catggccagc caaacgggca gggggaagat tttgatcgtg
     4801 gagctttcca gcgaggtgac gaaaaagtcc aggccgtagc gctcctcgag cgccgtccgg
     4861 aatttttccg ggaccttgag ccgcccgctt ttgtcgagct tggcccggta gcttccgatc
     4921 agtaagtttc ccattttgtc ccgttttgtc ccattttcga gtcaaatata gaagaatcag
     4981 atggatttgt caagggaaat caactttttt cgagagagtt gaaagggggg gtataatgcg
     5041 gctaagtaat taataataaa gataatatat tgattcgtgg gataaaacgg gaaaaacaag
     5101 catttttttt ggaatttccc gggggatttt aagcggaatt cagggatttt caagctgggg
     5161 gcggcggcgc cgttttgccg cgctttcagc gctcgcaagg cgccgctttc ggtcgtatcg
     5221 ggcggtgctt cgcggccgaa aaataacgaa gaggggactc gaacccctac gagcggtgtg
     5281 ggctgtaacc tgtccgttta taacatgagc gcgcccaaaa aagagcagaa caaatggtct
     5341 attagctcag tcggtacaga ctgatgtttc aatcgttccg tccgcaaggg cgtgattaga
     5401 ccaccgacct gatgattacg aatcagcagg tctttgctca atccgttccg cccggcgaag
     5461 gatgcgacga accgccggcc tggggaattt caacctatcg cgcgccggca gggtttcaat
     5521 ccacgcgtcc cgcgcgggac gcgacccgcc atgatcgttg ccgcggtatg gcggagccca
     5581 tgtttcaatc cacgcgtccc gcgcgggacg cgacgccatg atcgttgccg cggtatgacg
     5641 gagcccg
//
LOCUS       DAOOXH010000126         5633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_16634_length_5633_cov_7.490498, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000126 DAOOXH010000000
VERSION     DAOOXH010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16634_length_5633_cov_7.490498"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            394..1722
                     /locus_tag="PKV64_10710"
     CDS             394..1722
                     /locus_tag="PKV64_10710"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPN17585.1"
                     /translation="MKRRIAVFVAVFLIVLGFVYGQKKPYSLGQRPGQNVSDDPRRIP
                     MPEPDLSKAAVQVLRGATLIDGTGANPISNAVIVIRGNRIQEVGRLGQVTIPEGAALI
                     PAEGKYILPGMIDLHTHITYPTNVLEFFTDTDITATLRAVDKIKYYSNHGITSLRDVG
                     SRDDVPFRLKEAVRSGLVTGPRIFPAGKLITGTGGHGAEGGYLIVNGIVREANGADDF
                     RKAVREQIKAGADCIKLASHYTLEEAAAAIDEAHQMGLRVTADSHTFYITWAVQAGID
                     GIDHPLPRDEETIRLMAEKGTCAVPTIVAYYHIFQERGGYWGTQSRRFYFDHAANMEL
                     LRRLKKAGIKTGIGTDVIMDYTTQLPKYYLMELRFFLEAGYTPMETIIAATRTGAEIL
                     GMADKLGTIEKGKLADLIVVEENPLEKIDAVVQPSLVMVDGKIVNKHGED"
     gene            1873..2946
                     /locus_tag="PKV64_10715"
     CDS             1873..2946
                     /locus_tag="PKV64_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HPN17586.1"
                     /translation="MRTRARSLIVLILATALAPAAARPCTTFCLKTKDGGVFYGRNFD
                     FPTGFGQIQVNPRGLKKTSLANPPEKPLTWVSKYGSISFNQNGREFPYGGMNEAGLVI
                     EQMWNNNGTYPESDSRSGLGELQWIQYQLDMSASVADVLASDRLVRISNTSLAPLHFL
                     IADAWGDAATLEYVGGKTVVHAGKDLPYPVLANNSYDVSLDFTSHGETGDPKAFDDAT
                     RESSGRFARAAAMVEADKGGSEGAIDRTFAILAAVTGTNTQWSIVYDLGKRTIIYKTR
                     NNKNLRRVEMDRFDFSCSRNWLCVDIEDSVRSSADFKLYTDEGNAKLIDNVWESVEFL
                     KGVPMEVRAAFVGYPATVRCAED"
     gene            3179..5425
                     /locus_tag="PKV64_10720"
     CDS             3179..5425
                     /locus_tag="PKV64_10720"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPN17587.1"
                     /translation="MQRLFGALLALVFASPALPGRQSPAPAGNPQAESAAPYVLPRIQ
                     GKVTLDGLSSEPAWKGIRALPVLVYTPNTGAEPSELTEILVAYDDEFLYVAGRLYDRE
                     PEKIQATSKKRDDMKLSNDWLGIVLDTFHDRENGLAFFTTPAGLRLDMTIFHDADGDF
                     PVNQNWNTFWDVKTVRNQEGWFVEMRIPLSSLRFQEVEGRVVMGLTAWRYIARKNEVI
                     TFPAIPPKWGFWGSFKPSQTREIVLEGVSSRKPLYAAPYLLGGAGRTEALNEAETAYV
                     GTNKLVREAGLDVKLGLTNNLTLDLTGNTDFAQVEADDQQVNLTRFSLFFPEKRLFFQ
                     ERASVFDFNYGDESSLFYSRRIGLHEGKLVRIYGGARLIGRIGPWDLGLLDMQTAAVE
                     DLPSQNFGVLRVRRQVFNPNSWVGAMATCRIGVDGTFNTAYGLDGIVKLFGDDYLSWN
                     WAQTFAAGKRNRLLSLAPAKVRVNWQRRTVEGLAYNLDFSRAGAGYEPGLGFEFRSDY
                     SRFGDRLLYGWLPGEKSWLLRHSVFLDGFLYLRNKDGAVESSEVGPGYQFTAKSDFYV
                     MVRPAFFFENVSEAFSLSDEAEIPAGRYRFFTTKLAFNTPTAASFYLEGSLEAGSFYD
                     GNLVSAGISPRWDVSSGLEVGGFYGFNRAAFPERNQKFIAHIGRLSLLVMLSTRFSAA
                     AFIQYSSEADTLTSNIRIRYNPREGNDFYLVYNDGLNTDRLRERPPLPRSVGRTLLLK
                     YTYTFNFR"
     gene            5494..>5633
                     /locus_tag="PKV64_10725"
     CDS             5494..>5633
                     /locus_tag="PKV64_10725"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HPN17588.1"
                     /translation="MLRILREFGFSAVSIRGSHAKLKREAEGSPTHVLTVPLHRELAP
                     GTV"
BASE COUNT         1150 a         1725 c         1718 g         1040 t
ORIGIN      
        1 agcgtccgaa cggcgggggc cgggatcacg gccccattcg cgtcccaggg attccacggg
       61 ggcggcctgg gcgctgcggg cggtttccat gagattccgt ggataaacga tttacgatgg
      121 gcagagctat cggcccgggc ggctttgacg atgcttatgg tcaagggact gccgagccct
      181 ggccgtggcc gagcgcctcc tctcttggcg gcctttataa gaatacggag ttacgataag
      241 attctgagac gacgcgggcc gtcctcaatc agccttgaca ggccgcggga gtaaaaatat
      301 cctcagcccc gaggataaga aacgaattcc tatcatgatg gggccggcca gcgcggccgg
      361 gagaaagctg aaatcgcgcg ggaggagaaa aacatgaaga gaagaatcgc cgttttcgtc
      421 gccgtctttc tcattgtctt ggggtttgtt tacgggcaga aaaagccgta cagcctcgga
      481 cagcgtcccg ggcagaacgt ttcggacgat ccccgacgca tccccatgcc cgaaccggac
      541 ctgtccaagg ccgcggtcca ggttcttcgg ggagcgactc tcattgacgg gaccggcgca
      601 aatccgatca gcaacgccgt catcgtcatc cgggggaacc gcatccagga ggtcggccga
      661 ctcggacaag tgaccatccc ggagggagcg gccctcattc ccgcggaagg caagtacatc
      721 ctgccgggaa tgatcgacct ccacacccac atcacctacc cgacgaatgt gctagagttt
      781 ttcacggaca cagacatcac ggcgacgctg agggccgtcg ataagatcaa gtattactcc
      841 aatcatggaa tcaccagcct gcgggacgtc ggttcccgcg acgacgtccc ttttcggctt
      901 aaggaggcgg tgcggtccgg cctcgtaacc gggccgcgga tattccccgc cggcaagctg
      961 atcacgggca cggggggaca tggagccgaa ggcggctacc tgatcgtcaa cggcatcgtc
     1021 agggaagcca atggcgccga tgatttcaga aaggcggttc gagagcaaat caaggccggc
     1081 gcggactgca tcaagctggc cagtcactat acgcttgagg aggcggccgc ggccatcgat
     1141 gaggcccatc agatgggcct tcgggtgacc gcggattccc ataccttcta catcacctgg
     1201 gcggtccagg ccggcatcga cggcatcgac catccgctcc cgcgcgacga ggagaccatc
     1261 cgcctgatgg ccgagaaggg gacctgcgcg gttccgacga tcgtggctta ctatcatatt
     1321 tttcaggaac gcgggggata ctggggcacc cagtcgaggc gcttttattt tgaccatgcg
     1381 gcaaacatgg aattgctgcg tcgcttgaaa aaagcgggga tcaagacggg catcggcacc
     1441 gacgtcatca tggactatac gacgcaactg ccgaaatatt atctcatgga gctgcgtttc
     1501 tttctggagg ccggttacac cccgatggag acgatcatcg cggccacgcg cacgggggcc
     1561 gaaatcctcg ggatggccga caagcttggg accatcgaga agggcaaatt ggcggacctg
     1621 atcgtggtcg aagaaaatcc gctcgaaaaa atcgacgcgg ttgtgcagcc ctcgctggtc
     1681 atggtggacg gcaaaatcgt caataaacac ggtgaagatt aggggcggca tcacctcttg
     1741 tctgaagtgc cggaaaccgc gagaacgacc cttccgtcca aggaagggcg aacacaagag
     1801 cccccggagg gggaaggaac tttcctcccg cgtttttcgt ctacgagaga gggtttcaaa
     1861 taaggagtct gtatgagaac cagagcccgt tccctcatcg tcctgatact ggcgaccgcc
     1921 ctcgctccgg cggccgcccg cccttgcacg acgttttgcc tgaagaccaa ggacggcggc
     1981 gttttttacg gccggaactt cgattttccg acgggcttcg ggcagatcca ggtcaatccc
     2041 cgaggcctga aaaagacgtc cctggccaat cccccggaga agccgttgac ttgggtttcc
     2101 aagtacggaa gcatttcctt caaccagaac ggacgggagt ttccctatgg cgggatgaac
     2161 gaggccgggc tggtcatcga gcagatgtgg aacaacaacg ggacctaccc cgagtcggac
     2221 agccgctccg ggctggggga gctgcaatgg atccagtatc agctggacat gtccgcatca
     2281 gtcgcggatg tgctggccag cgatcgcctc gtccggatat caaacacatc cctggccccg
     2341 ctccatttcc tcatcgccga cgcctggggc gatgcggcca cgctggaata cgtgggcggc
     2401 aagacagtcg ttcacgccgg gaaggacctg ccttatcccg tcctggccaa caacagttat
     2461 gacgtgtccc tggacttcac gtcgcatggg gagaccgggg atccaaaggc ctttgacgat
     2521 gccacccggg aatcctccgg ccggtttgca cgggccgcgg cgatggtcga ggcggacaag
     2581 ggcggaagcg aaggagcgat cgaccggacc ttcgcgatcc tggccgcggt gacgggaacg
     2641 aatacccaat ggagcatcgt ttacgacctg gggaaaagga cgatcatcta taagacccgc
     2701 aataacaaga acctccgccg ggtggagatg gaccgcttcg acttttcctg ttcgcggaat
     2761 tggctctgcg tcgacatcga ggacagtgtc cggagctcgg ccgatttcaa gctctacacc
     2821 gatgaaggca acgcgaagct gatcgacaat gtctgggaaa gcgtggaatt cttgaaaggg
     2881 gtccccatgg aggtccgagc ggcgttcgtc gggtatccgg cgaccgtccg ctgcgccgag
     2941 gactgagccg gccctccgcc cggccttgag atcccttgat cggtcttgaa ggcccgaatg
     3001 agcgcggcga tatcatatta ttccatccgg agaaaggcgt tggccacgtg ccccgctttg
     3061 cgcaggtcga ggttccggag catatcctct agagcccgga tcgacttggc tggacagtcc
     3121 gaacccgctt gacaatccgg aatcgcggcc tattaatatc attaaacgag gaaggggaat
     3181 gcaaaggctc ttcggagcgc tcctggcctt ggtctttgcg tcgccggccc ttcccggccg
     3241 gcaaagcccg gcgccggcgg ggaatccgca ggcggaaagc gccgcgccgt atgtactccc
     3301 ccggatccag ggcaaggtga cgctcgacgg gctgagctcc gagccggcct ggaaaggcat
     3361 ccgggcgctc cccgtcctgg tctacacgcc gaacacgggg gcggagccct ccgagctcac
     3421 agagatcctc gtggcctatg acgacgaatt tctctatgtc gccgggcggc tttatgaccg
     3481 cgagccggaa aagatccagg cgacatccaa aaaacgcgac gacatgaagc tcagcaacga
     3541 ctggctggga atcgtcctgg atacgttcca cgaccgggaa aacggcctcg cctttttcac
     3601 aacccccgcc ggcctccggc tggacatgac gatcttccat gacgccgacg gggacttccc
     3661 cgtgaatcag aactggaaca cgttttggga tgtgaaaacc gtccgcaacc aggaggggtg
     3721 gttcgtcgaa atgcggatcc cgttatccag cctccggttc caggaagtcg aagggcgggt
     3781 cgtcatgggg ctcaccgcct ggcgctatat cgcccgcaaa aacgaagtga tcacctttcc
     3841 cgccatcccg ccgaaatggg gcttctgggg aagctttaaa ccctcccaga cgcgcgagat
     3901 cgtccttgaa ggagtgtcca gccgcaagcc cctttatgcc gctccctacc tcctcggagg
     3961 cgccggccgg accgaggcgc tcaacgaggc ggaaacggcc tacgtcggca cgaacaagct
     4021 cgtccgcgaa gccggactcg acgtcaagct tgggctgacg aacaacctga ccttggatct
     4081 caccgggaat accgatttcg cccaggtcga agcggacgac cagcaggtga acctgacgcg
     4141 cttttccctg ttcttcccgg agaaaaggct cttcttccag gagagggcga gcgtcttcga
     4201 cttcaattac ggcgacgaaa gcagcctttt ctacagccgg cgcatcggcc tccacgaagg
     4261 gaagctcgtc cgcatctacg ggggggcccg cttgatcggc cggatcggtc cgtgggacct
     4321 cggccttctc gacatgcaga ccgccgccgt cgaggatctg ccctcgcaaa acttcggcgt
     4381 cctgcgcgtc cggcggcagg tcttcaatcc gaattcctgg gtgggggcga tggccacctg
     4441 ccggatcggc gtggatggga cgttcaacac ggcttacggc ttggacggca tcgtcaagct
     4501 cttcggcgac gattacctgt cgtggaattg ggctcagacc ttcgccgcgg ggaagagaaa
     4561 ccgcctcctt tcgctcgccc cggccaaggt ccgggtcaac tggcagcgcc ggaccgtcga
     4621 gggcctggct tataacctgg atttttcccg ggcgggcgcc gggtatgagc ccggcctggg
     4681 attcgaattc cggagcgact acagccgttt cggcgaccgt ctcctctacg gctggctccc
     4741 gggcgaaaag tcctggctcc tgcgtcacag cgtcttcctc gacggattcc tctacctgcg
     4801 gaataaagac ggggccgtcg agtcctccga ggtcgggccg ggataccagt tcaccgcaaa
     4861 atccgacttt tatgtcatgg tccggccggc cttcttcttc gagaacgttt cggaggcctt
     4921 ttccctgtcc gacgaggccg agatcccggc cggccgatac cgctttttta cgacgaagct
     4981 ggccttcaac acgccgaccg cggcgagctt ttacttggaa ggctcgctgg aggccggctc
     5041 gttttacgac gggaatctcg tctcggcggg aatttcgccg cgctgggacg tttcgagcgg
     5101 cctggaagtc ggcggcttct acggattcaa ccgggcggcg ttccccgaaa gaaaccagaa
     5161 gttcatcgcc catatcggac ggctgagcct tctggtcatg ctgagcaccc gcttttcagc
     5221 cgccgctttc atccagtaca gcagcgaagc cgacacgctg acgtcgaaca tccgcatccg
     5281 atacaacccc cgggaaggga acgatttcta tctcgtctat aacgacggat taaacaccga
     5341 ccgcttgagg gaaaggccgc cgctgccccg gtccgtgggc cgcacccttc tcctgaaata
     5401 tacctacacc ttcaacttcc ggtaggatcg ccgtgaccta cgaagaaaac ccgtccggcg
     5461 agggccaggc ttaggcgtct atcggcccgg cagatccttc ggatcctccg ggagttcggt
     5521 ttttcggccg tttcgatccg gggcagccac gccaagttga agcgcgaggc cgaagggagc
     5581 ccgacacatg tcctgacggt ccccctccat cgggaacttg cccccggaac ggt
//
LOCUS       DAOOXH010000127         5522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_17089_length_5522_cov_5.874154, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000127 DAOOXH010000000
VERSION     DAOOXH010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17089_length_5522_cov_5.874154"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PKV64_10730"
     CDS             <1..595
                     /locus_tag="PKV64_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPN17589.1"
                     /translation="AGRRPNSGGLSEGASPVALDKRGFVQVNERLETSVPGIYAVGDL
                     IGGKLLAHKASHEGIAAAENAAGGRGHVDYRALPMAVFTEPEFASIGLTEEEARAEGG
                     EVRIGTFSFQANGRALTMNSPDGLAKIIAGADGRILGAHVLGACASDLIAEITLAMTR
                     GLKASDIGESIHIHPTLSEAVMEAALKVDGRAIHCLN"
     gene            640..981
                     /locus_tag="PKV64_10735"
     CDS             640..981
                     /locus_tag="PKV64_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HPN17590.1"
                     /translation="MTLALVFLVFGLLGAVMISLNALLGPRKTNPVKEQPFECGSPPL
                     QKGIPGFAVKFPLVVFLFVLFDIEVAFLFLWALVFREMKGPAFLLLLAYTAVLAAGFV
                     YAWRKGAFRWE"
     gene            986..1462
                     /locus_tag="PKV64_10740"
     CDS             986..1462
                     /locus_tag="PKV64_10740"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HPN17591.1"
                     /translation="MAGGFLTTRFDALLSWGRKYSLFHYPFITACCGMEYMSTACAHY
                     DIDRFGAGMTRFSPRQADMLLVVGTINRKLAPVLKTVYDQMTEPKWVMAFGACTVSGG
                     IYDNYAVVQGIDEIIPVDIYIPGCPPRPETVLDGLLKLQEKIKAEGQDWRWKKKTS"
     gene            1441..1932
                     /locus_tag="PKV64_10745"
     CDS             1441..1932
                     /locus_tag="PKV64_10745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPN17592.1"
                     /translation="MEEKDILTVLREALPGAVLEEGESFGDPVVRIRPEALLPAAGLL
                     KAEPPGYAMLLDATCVDYPDRPGRFEMVYHFFSLARNRRIRVKADLAAGRPEIASLAS
                     MWKNAAWLEREIFDMFGVRFTGHPGLRRLFLDEGFEGHPLRKDFPLRGRQPLPSNSKQ
                     ETP"
     gene            1929..3254
                     /locus_tag="PKV64_10750"
     CDS             1929..3254
                     /locus_tag="PKV64_10750"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HPN17593.1"
                     /translation="MTSPLVSAARPENLLINMGPSHPAMHGTVRIMLELDGERVQNAD
                     VEVGYLHRGFEKTCENRTWWNLLIYTDRLNYVSPLLNNLGYALAVEKLCGLGIPERAQ
                     WIRVLMGEISRICDHLTCLAAMAMETGAFTAFLYNMKAREFLWDVIEQTTGARLTTSY
                     IRIGGVRADLQADYESAIRRGIEETRRVLRDVRRLLDRNPIFLGRTRDVAAMSREEAV
                     AHGWTGPALRSTGLAYDVRKARPYLTYDRLEFEIPTGDRGDNYDRYLVRMAEMEQSLR
                     IIEQCLGPLRRERPAAKAAAIEPAEAIQAARTSPPEAPVRLSPTLEGAERDVLGGIQT
                     SAPGYGLPGKDAVYGTIEGLISHFQYFMKGHGLRPPAGEVYAAIEGGNGELGFYLVSD
                     GTDRPYRLHLRAPCLHVVSALAGLIQGRLLADIIPTFGSMNMIGGELDR"
     gene            3251..3724
                     /gene="nuoE"
                     /locus_tag="PKV64_10755"
     CDS             3251..3724
                     /gene="nuoE"
                     /locus_tag="PKV64_10755"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPN17594.1"
                     /translation="MTNAFPSGLRTRIEALLARYPNRRAALLPVLHLVQRERGFIGPE
                     DEAEAAVLCGLRPVEVREVLTFYTMFRREPAGRHLLRVCTNLSCSIRGSAGILDHLRR
                     AFGLQPGRTTPDGRFTLVEVECLGACDKGPCLLAGDTLHENLTPAALDAILRELE"
     gene            3728..4963
                     /gene="nuoF"
                     /locus_tag="PKV64_10760"
     CDS             3728..4963
                     /gene="nuoF"
                     /locus_tag="PKV64_10760"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPN17595.1"
                     /translation="MAERPLTSTFDPAGPRSLEAYVRGGGYAAARRALAEMTPAAVAG
                     EVKAAELRGRGGAGFPAGVKWSFIPRTDPGPFYLCVNADEGEPGTFKDRHILAHDPHL
                     LLEGIILCAYAVGVHTAYIYIRGEYEEPARRLEKAIAEAAGAGVLGASVLGSGFSLDV
                     IVHRGAGAYICGEETALLESLEGFRGQPRLKPPFPAAVGLFGKPTVINNVETLAHLPA
                     VIRNGASWFLGLGKPKDGGTRLVSVSGSVVRPGVYELPVGTPLRDVVFVHAGGLAPGR
                     TLKAVIPGGMSSAVLGPGEIDVPMDSASLAAAGSMIGSAGVIVIDDAVSMKTVLDRIS
                     RFYAHESCGQCTPCRVGTSWIRRIVLRRGAGEGRAGDLDRIVRLAGAMKGRTLCPFGD
                     AAAVPILALVTKFRAELEQ"
     gene            4968..>5522
                     /locus_tag="PKV64_10765"
     CDS             4968..>5522
                     /locus_tag="PKV64_10765"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPN17596.1"
                     /translation="MVKVTIDGRVLETAEGTTILAAAEQAGISIPRFCHHPALTPEGS
                     CRMCVVEIEGAAKLEPACATAVREGMKVATQTPQVAEARRNVLELLLADHPVDCPICD
                     KAGECRLQDYYREYGLFPSDLHEPKTRREKLLKIGERLILDRERCVLCTRCVRFLREI
                     TKTGELGVFGRGGGSEIGFIEGAAV"
BASE COUNT          896 a         1831 c         1833 g          962 t
ORIGIN      
        1 cgccggccgg cggccgaatt ccggcgggct ctcggaaggc gcctctccgg tggccctcga
       61 caagaggggc tttgtccaag tcaacgagcg tttggagaca agcgtccccg gcatttacgc
      121 cgtcggcgac ctgataggcg gaaaactcct ggcccacaag gcctcgcacg agggaatcgc
      181 ggccgccgag aacgcggccg gggggcgcgg ccatgtcgat taccgggccc tgcccatggc
      241 cgtcttcacc gagccggaat tcgcctcgat cggcctgacc gaggaagaag cccgggcgga
      301 agggggcgag gtccggatcg gcacattctc cttccaggcc aacggccggg cgctgaccat
      361 gaattccccc gacggcctgg ccaagatcat cgccggggcg gacggccgga tcttgggcgc
      421 ccacgtgctc ggcgcctgcg ccagcgactt gatcgccgag atcaccctgg ccatgacccg
      481 gggcttgaag gcctccgata tcggggagtc gattcatatc catccgacgc tctcggaagc
      541 ggtcatggaa gcggccttga aagtcgacgg acgggcgatc cattgcttga attgagaaat
      601 gggattaaaa tcctgccgct cccgcgagga taatgctcca tgaccctggc cttggtcttc
      661 ctggtgttcg gccttctcgg cgcggtcatg atttccctga acgcgcttct cggcccgcgg
      721 aagaccaacc ccgtcaagga gcagcctttc gaatgcggca gcccgccgct tcagaagggg
      781 atccccggtt tcgccgtcaa gtttccgctc gtcgtcttcc tcttcgtcct gttcgacatc
      841 gaagtcgcct ttctcttcct ctgggccctc gttttccggg aaatgaaggg accggcgttc
      901 cttctcctcc tcgcctacac cgccgtgctg gcggccggat tcgtctatgc ctggagaaag
      961 ggggccttcc gctgggaata gagccatggc cggaggattc ctcaccaccc gcttcgacgc
     1021 cctgctttcc tggggccgca aatactcgct cttccattat cccttcatca cggcctgctg
     1081 cgggatggaa tacatgtcga cggcctgcgc ccattacgac atcgaccgct tcggcgcggg
     1141 catgacccgg ttttcaccgc gccaggcgga catgctgctc gtcgtcggaa cgatcaaccg
     1201 caagctggcc cccgtgttga aaacagtcta cgaccagatg accgagccga aatgggtcat
     1261 ggccttcggg gcctgcacgg tttcgggcgg catctacgac aattacgccg tcgtccaggg
     1321 aatcgacgaa atcatcccgg tcgacatcta catccccggc tgtccgcccc gcccggagac
     1381 ggtgctcgac ggcctcctca agctccagga aaaaatcaag gccgagggcc aggactggcg
     1441 atggaagaaa aagacatcct gaccgtcctg cgcgaggccc ttcccggcgc agtcctcgag
     1501 gaaggagaat cgttcggcga tccggtcgtt cggatccgcc cggaggcgct gttgccggcg
     1561 gccggccttc tcaaggccga gccgcccggg tacgcgatgc tgctcgacgc gacgtgcgtc
     1621 gattatccgg accgccccgg ccgcttcgag atggtctatc atttcttttc cctcgcccga
     1681 aaccggcgga tccgggtcaa ggcggatctt gcagccggcc ggcccgaaat cgcctcgctc
     1741 gcctcgatgt ggaaaaacgc cgcttggctc gagcgggaga tcttcgacat gttcggcgtg
     1801 cgcttcaccg gccatcccgg cctccggcgc cttttcctgg atgaaggatt cgaggggcat
     1861 ccgctccgca aggatttccc cctccgcgga cgtcagccgc tgccttcgaa ttcgaaacag
     1921 gagacgccgt gacctccccg ctggtttcgg ccgcccgccc cgagaacctg ctgatcaaca
     1981 tggggccgtc ccacccggcc atgcatggaa cggtccggat catgctcgag ctcgacggcg
     2041 agcgggttca aaacgccgac gtcgaggtgg ggtatctcca ccgcggcttc gaaaagacct
     2101 gcgagaaccg gacctggtgg aatctcctga tctacaccga ccggctgaat tacgtctcgc
     2161 cgcttctcaa caacctcggc tacgccctgg ccgtggaaaa gctctgcggg ctggggatcc
     2221 ccgagcgggc tcagtggatc cgggtgctga tgggggagat ctcgcggatc tgcgaccact
     2281 tgacctgcct ggcggccatg gccatggaaa ccggggcctt caccgccttc ctttacaata
     2341 tgaaggcccg tgagttcctc tgggacgtca tcgagcagac gaccggcgcc cggctgacga
     2401 cgtcctacat ccggatcggc ggcgtgcggg ccgacctcca ggctgattac gaatccgcca
     2461 tccgccgtgg aatcgaggaa acccggcgcg ttctccggga cgtccgccgc cttctggacc
     2521 ggaatccgat ctttctcggc cggacgcgcg acgtcgcggc gatgagccgc gaggaggcgg
     2581 tggcccacgg ctggaccggg ccggcccttc gctcgacggg cttggcctac gacgtccgga
     2641 aggcccggcc ttacctgacg tacgaccgcc tggaatttga aatcccgacc ggcgaccggg
     2701 gcgataatta cgaccgatac ctcgtccgga tggccgagat ggagcaaagc ttgaggatca
     2761 tcgagcagtg tcttggcccg ctccggcggg agcggccggc cgccaaagcg gccgcgatcg
     2821 agccggccga ggcgatccag gccgcccgga cgtctccgcc cgaggccccc gtccgcttgt
     2881 ccccaacgct cgagggcgcc gaacgggatg ttctcggcgg catccagaca tccgctcccg
     2941 gatacggctt gcccggaaaa gacgccgtct acggaacgat cgagggcttg atctcccatt
     3001 tccaatattt catgaaggga cacggcctgc ggccgccggc cggggaggtc tacgcggcga
     3061 tcgagggggg caacggcgag ctgggatttt acctcgtttc cgacggaacg gaccggcctt
     3121 accggctcca cctgcgggct ccgtgtcttc acgtggtctc cgccctggcc ggcttgatcc
     3181 aggggcggct gctggcggac atcatcccga ccttcggctc gatgaacatg atcggcgggg
     3241 agctggaccg gtgacgaacg cgtttccctc gggactgcgg acccggatcg aggcgctcct
     3301 cgcccgctat ccgaaccggc gggccgctct tcttcccgtc ctccaccttg tccagcggga
     3361 acgggggttc atcggtccgg aggatgaggc cgaggccgcc gtcctctgcg gcctgcggcc
     3421 ggtcgaggtc cgggaagtct tgacgtttta cacgatgttc cggcgcgagc ccgccggccg
     3481 ccatcttctg cgcgtctgca cgaatttgtc ctgctccatc cggggaagcg ccgggatcct
     3541 ggaccatctc cgccgcgcgt tcggcctcca gccgggacgg acgacgccgg acgggcgctt
     3601 caccctggtc gaagtcgagt gtctcggggc ctgcgacaag gggccgtgcc tgctggccgg
     3661 agacacgctc catgaaaacc tgacgccggc cgccctggac gcgatcctgc gggagctgga
     3721 gtgaccgatg gcggaaagac ccctcacctc gacgttcgac ccggccggcc cccggtcgct
     3781 ggaggcctac gtccgcggcg gaggctacgc cgcggcccgc cgggccctgg ccgaaatgac
     3841 accggccgcg gtagccggcg aagtcaaggc cgccgagctc cgaggccggg gaggggcggg
     3901 ttttccggcc ggggtgaaat ggagctttat tccccggacg gatccgggtc ctttttatct
     3961 gtgcgtcaac gcggacgagg gagagccggg gacgttcaag gaccggcaca tcctggccca
     4021 cgatccgcac ctcctgctcg agggaatcat tctctgcgct tatgccgtgg gcgttcatac
     4081 ggcgtacatc tatatccggg gcgaatacga ggagccggcc cggcgcttgg agaaggcgat
     4141 cgccgaagcc gccggggcgg gcgtcctggg agcgagcgtc ctcggctcgg gcttttccct
     4201 ggacgtgatc gtccaccgcg gggccggggc ctacatctgc ggcgaggaga cggctctcct
     4261 cgaatccctc gagggattcc gcgggcagcc gcgcctcaag cctcccttcc cggcggcggt
     4321 cgggctgttc ggcaagccga cggtcatcaa caacgtcgag acgctggccc atctgcccgc
     4381 cgtcatccgg aacggggcgt cttggtttct cggtctcgga aaaccgaagg acggggggac
     4441 gcggctggtc agcgtcagtg gatcggtggt ccggccgggc gtctacgagc tcccggtcgg
     4501 gacgccgctg cgggacgtcg ttttcgtcca tgccggcggc ttggctcccg gccggacgct
     4561 caaggccgtg attcccggag gaatgtcgtc ggccgtcctg gggcccggag aaatcgacgt
     4621 gcccatggac agcgcctcgc tggccgcggc cggctcgatg atcggatcgg ccggagtcat
     4681 cgtgatcgac gacgcggttt cgatgaagac ggtcctggac cggatcagcc ggttttacgc
     4741 ccatgaatcc tgcggtcaat gcaccccctg ccgggtggga acgtcctgga tccggcggat
     4801 cgtcctccgg cgcggggccg gagagggccg ggccggcgat ctcgaccgga tcgtccggct
     4861 ggccggggcg atgaagggac ggacgctctg cccgttcggg gacgcggcgg ccgttccgat
     4921 cctggctttg gttacaaaat tccgagcgga attggaacaa tagcgccatg gtcaaagtga
     4981 cgatcgacgg acgggtcctg gagacggccg agggaacgac gatcctggcc gcggccgaac
     5041 aagccgggat ttccatcccc cgtttctgcc atcatccggc cttgactccc gaaggctcct
     5101 gccgcatgtg cgtcgttgag atcgagggcg cggccaagct cgagccggct tgcgcgacgg
     5161 ctgtccggga ggggatgaag gtcgcgacgc aaacgccgca ggtggccgag gcccggcgga
     5221 acgttctcga gctcctcctg gccgaccatc cggtcgactg tcccatctgc gacaaggcgg
     5281 gcgagtgccg gctccaggat tattaccggg aatacgggct cttcccgagc gatctccacg
     5341 agccgaaaac acggcgggag aagcttttaa aaatcggaga gcggctgatc ctggaccggg
     5401 agcgctgcgt cctttgcacc cggtgcgtcc ggtttctgag ggagatcacc aagacgggag
     5461 agctgggcgt tttcggccgg ggcggcgggt cggagatcgg cttcatcgag ggggcggcgg
     5521 tg
//
LOCUS       DAOOXH010000128         5434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_17438_length_5434_cov_7.162484, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000128 DAOOXH010000000
VERSION     DAOOXH010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17438_length_5434_cov_7.162484"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..2140)
                     /locus_tag="PKV64_10770"
     CDS             complement(122..2140)
                     /locus_tag="PKV64_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPN17597.1"
                     /translation="MKRLRFFLPLMAAVLTVPPLSAQVAFDRYHPPAEMAAAFRDLAR
                     SHPDFVKIHELAESPGGREVLVIELGPEAGRTVKTLPAVFVGADFEGLHPLAGEAALY
                     LVGRIVDKPEVRRDLTWYVLGCPNPDAAARFFAKPLWADPGNGRPWNDDMDDAVDEDG
                     PEDLDGDGLITTMRIVDPGGEWLPAPGQPRLLKKADWAKGEQGIYKLLPEGLDNDRDG
                     AYNEDPPGGVNIGVQFPHLFAAFKPGSGLWPGSEEESFAVLKFVNEHKEIGLAVVFGG
                     ANFCLNPPPGGRRGDADLNRIRVPKDMAGFINADPDKDYTMEELLELAKASLPEGMTV
                     DVSLIASFLGLGAAVNPLPEDLKFYAELSDKYKEFLKAARLDEKRLAPAADKDGSFEL
                     YAYYHLGLPSFALDFWTLPEAREEKAAPGLAPGELEKMTGEEFIALGEEKIAAFLKTS
                     GAPPEFTAAQAIEAIKTGRTSTKEMAAMMMRTPPPSSAEGADPRDKARLAWSDKEPAG
                     RAFVDWKPFKHPVLGDIEIGGAVPYADTTPPPAMIEPLLREQVPWVFELASRMARIRL
                     GPVTIRPLGGGLHEIEAWIENAGYLPYPTAMGRRNNRIFPVIVTLEGRDLAFIEGRPR
                     TAVPAVDGSGRRKIRWIVRSPKPVKIELRAAAPSAWGDVRTLELGGGK"
     gene            complement(2326..3150)
                     /gene="murI"
                     /locus_tag="PKV64_10775"
     CDS             complement(2326..3150)
                     /gene="murI"
                     /locus_tag="PKV64_10775"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPN17598.1"
                     /translation="MDIAGPIGVFDSGYGGLTVFREIAAVLPDCDFVYLGDNARTPYG
                     TRSFETVHRFTWEAVRWLFGRGCRLVILACNTASAKALRTIQQKDLPAEHPGRRVLGV
                     IRPVTERVGTLSRSRHVGLFATAGTVRSQSYPLEIVKFFPDLVVHSEACPFWVPLVEN
                     NELDGPGTEFFVRRHVERLLALDPGIDAIILGCTHYPLLAPVIARFLPPAVKLISQGP
                     LVAAALKDYLKRHPEIDGACTKGGRREFWTTDDPGDFDRLAGLFYGASVRSEKAVL"
     gene            complement(3161..4489)
                     /locus_tag="PKV64_10780"
     CDS             complement(3161..4489)
                     /locus_tag="PKV64_10780"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPN17599.1"
                     /translation="MRWKNAGPKTNLFKNARWLLIPAVLAAGAWLWIVKFESEKPAVR
                     LLRDGPALGRELSLRFEDRKSGLARIRVEADQAGRTVELLSEEFPEPVALVEKTISLR
                     PLPAGLTDGEIILRIAASDHSWRKGNAAVWEMRKIIDTRPPRMTFLGGPHSIAPGGAG
                     VVSFMLDESAALSGLQAGNSFFPAYEAGGGRYFVWYALPCDAPPDVPFIGVAEDEAGN
                     RTNVRFRPDVKSVRIKSDVLTLTDAFFEGVVPYFKSLDPSLKGTDFEIFLAVNRDQRA
                     RDDERLQAICRDTGPRPLWRGTFLRLPRSKTMAAFGERRTYLYNGREIDRQIHLGVDL
                     ASVRNCPVPAANAGRVVFAGPLGIYGETVILDHGCGLFSMYCHLSRIDAAVSSSVARG
                     DVLGLSGATGMAGGDHLHFAMLVHGVFVDPVEWWDARWIQNNVAIKMESE"
     gene            4700..>5434
                     /locus_tag="PKV64_10785"
     CDS             4700..>5434
                     /locus_tag="PKV64_10785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HPN17600.1"
                     /translation="MKLYAHSKADTPQNQWHPLEEHLHATANNARHFAETWESGAWAY
                     NAGLLHDLGKSDSRFQGYLLRCNELDDSDYDSGRVNHSSTGASCAEDGIKPFGRILAY
                     LIAGHHAGLPDWFSSESANAALSMRLKEGRENSKRIRAFSEELILSLLPMTKPSFVNA
                     ANFHFWARMLFSCLVDADYLDTEQFMDEEKSRQRSHYPYLSSLAPRLFDFLDDLEQKA
                     PQSSVNEIRAEIRRACERKADSPKGLF"
BASE COUNT         1087 a         1665 c         1696 g          986 t
ORIGIN      
        1 tcccccactt cggccaacgt gtagtaatgg tcgaaccgaa gcggaagcgc ggcctcggcc
       61 cggacggccg gggccgccgc ggacacgatc aagaacgcca ggagcgccgg cgcgatgttt
      121 ttcattttcc acctccaagc tcgagagtcc ggacgtctcc ccaggcgctg ggagcggcgg
      181 cccggagctc gatcttcacg ggcttgggcg accgcacaat ccaccggatc ttccgccggc
      241 cgctgccgtc gacggccggg accgcggtcc gcggccggcc ctcgatgaag gccaaatccc
      301 ggccttcgag cgtgacgatc accgggaaga tccggttgtt ccggcgtccc atggccgtcg
      361 gatacggcag gtagccggcg ttttcgatcc aggcctcgat ctcatggagc cccccgccga
      421 gcgggcggat cgtcaccggc ccgagccgga tgcgggccat ccgggaagcc agttcgaaaa
      481 cccaagggac ttgctcccgg agcagcggct cgatcatcgc cggcggcgga gtcgtgtcgg
      541 cgtagggaac ggccccgccg atctcgatgt cccccaggac cgggtgcttg aacggcttcc
      601 agtcgacgaa cgcccttccg gccggttcct tgtcgctcca agccagccgg gctttgtccc
      661 ggggatcggc cccctcggcc gaggaaggcg ggggcgtccg catcatcatc gcggccattt
      721 ccttggttga ggtccggccg gtcttgatcg cttcaatcgc ttgggcggcc gtgaattccg
      781 gcggagcgcc ggacgttttc aggaaggcgg cgatcttctc ttcgcccagg gcgataaact
      841 cctcgccggt catcttctcc agctcgccgg gagcgaggcc gggagcggct ttttcctccc
      901 tcgcttcggg cagagtccag aagtcgagag cgaacgaggg caggcccaga tgataatagg
      961 cgtacagctc gaacgagccg tccttgtcgg ccgcgggggc gagacgcttt tcatcgagcc
     1021 gggcggcctt gaggaattcc ttgtacttgt ccgagagctc cgcgtaaaat ttcaagtcct
     1081 cgggcagcgg attcacggcc gcccccaggc cgagaaagct cgcgatcaac gagacatcca
     1141 ccgtcattcc ctcgggaagg gaggctttgg ccagctccag gagctcctcc atggtgtaat
     1201 ccttgtcggg gtcggcgttg atgaacccgg ccatgtcctt gggaacccga atccggttca
     1261 gatcggcgtc cccgcgccgg ccgccgggcg gaggattcaa gcagaaatta gctccgccga
     1321 agacgacggc caggccgatt tccttgtgct cgttcacgaa cttgaggacg gcgaagcttt
     1381 cctcctcgct ccccggccac aagcccgaac cgggcttgaa agcggcgaac aagtggggaa
     1441 actggacgcc gatgttcact ccgccgggag gatcctcgtt gtacgcgccg tcccggtcgt
     1501 tgtccaagcc ctcgggtaga agcttgtaaa tcccctgctc gcccttggcc caatcagcct
     1561 tcttcaggag gcggggctgg ccgggcgcgg ggagccactc gccgccgggg tccacaatcc
     1621 gcatcgtcgt gataagaccg tcgccgtcga gatcctcggg gccgtcctcg tcgacggcgt
     1681 catccatgtc gtcgttccac ggccggccgt tgccgggatc ggcccaaagc ggcttggcga
     1741 aaaagcgggc ggccgcgtcg ggattgggac aacccaggac gtaccaggtc agatcccggc
     1801 ggacctcggg cttgtcgacg atccgcccga cgaggtacaa ggccgcctcg ccggccagcg
     1861 gatggaggcc ttcaaaatcg gccccgacga aaacagcggg aagcgttttc accgtccggc
     1921 cggcctcggg acccagctcg atcaccagga cctcgcggcc gccgggactt tcggccagct
     1981 catggatctt gacgaagtcc gggtgggagc gggccaaatc gcggaaggcc gcggccattt
     2041 ccgcgggcgg atggtagcgg tcgaacgcca cctgggcgga aaggggcggg acggtcagca
     2101 cggcggccat caggggaagg aaaaagcgga ggcgtttcat cgtcacctcg tgatcggatc
     2161 attcaaaatc ggctttcccc gaaaggaaag agggaagatt acatcatcaa atccggagac
     2221 acaacaccaa ttccccttat ttccccgccg gcccggtcgg agggcgggaa aataagcaag
     2281 gcgggcgggg aatgagggaa atgggtattg agtctcttta atagactaga ggacggcttt
     2341 ttcggaacgg acggaagccc cgtaaaacaa gcccgccagc cggtcgaagt cccccggatc
     2401 gtcggtcgtc caaaattccc tccggccgcc tttcgtgcag gctccgtcga tctccggatg
     2461 acgcttgaga taatccttca aggcggccgc gacgagcggg ccctgggaaa tcagcttgac
     2521 cgcgggcggc aggaaccggg cgatcaccgg ggccaggagc gggtagtggg tgcagccgag
     2581 gatgatcgcg tcgatcccgg gatcgagggc caggagacgc tcgacatggc ggcggacgaa
     2641 aaattccgtc ccggggccgt ccaactcgtt gttttcaaca agcgggaccc agaacgggca
     2701 ggcttcggaa tggacgacca ggtccgggaa gaacttcacg atctcgaggg ggtaggattg
     2761 ggaccggacc gtcccggcgg tcgcgaacag cccgacatgc cggctccggc tgagcgtccc
     2821 gacgcgctcg gtgaccggac gaatgacgcc gaggacccgc cggccgggat gttcagcggg
     2881 aagatccttc tgctggatcg tccggagggc tttggccgag gcggtgttgc aggccaggat
     2941 gaccagccgg cagccccggc cgaacagcca gcggaccgcc tcccaggtga agcgatggac
     3001 agtctcgaac gatcgcgtcc cgtagggcgt ccgggcattg tcgcccaagt agacgaaatc
     3061 gcagtccgga aggacggcgg cgatttcccg gaaaacggtc aacccgccgt accctgaatc
     3121 gaaaaccccg atcggcccgg cgatgtccat cggaatcatc ctactccgat tccattttga
     3181 ttgcgacgtt gttttgaatc cagcgggcgt cccaccactc gaccgggtcg acgaagactc
     3241 cgtggacgag catggcgaaa tggaggtggt cgcccccggc catccccgtc gcccccgaca
     3301 agccgaggac gtcgccccgg gccacggatg aagacacggc cgcgtcgatg cggctcaggt
     3361 ggcagtacat gctgaacaat ccgcagccgt ggtccaggat gaccgtctcc ccgtaaatgc
     3421 ccagcggccc ggcgaaaacg acccggccgg cattggccgc cggaaccggg cagttcctga
     3481 ccgaagccag gtccacgccg agatgaatct gccggtcgat ctcccgcccg ttgtaaagat
     3541 aggtccggcg ctctccgaag gcggccatcg tcttggaccg gggcaagcgg agaaacgtcc
     3601 cgcgccacaa cggccggggc ccggtgtcgc ggcagatcgc ctggaggcgc tcgtcgtccc
     3661 gggcccgctg gtcccggttg acggccagaa atatctcgaa atccgtcccc ttgagggagg
     3721 gatcaaggct cttgaaatag ggaacaacgc cctcgaagaa cgcgtcggtg agagtcagga
     3781 cgtcgctttt gatccggacg gacttcacgt ccggccggaa acggacgttg gtccggtttc
     3841 cggcttcatc ctcggccacc ccgatgaagg gaacgtccgg cggggcgtcg cacggaaggg
     3901 cgtaccaaac gaaataccgg ccgccgccgg cttcgtaggc ggggaaaaag gaatttccgg
     3961 cctggaggcc ggacaaagcg gcgctttcgt caagcatgaa ggagacaacg cccgctccgc
     4021 cgggagcgat ggaatgaggc ccgccgagga acgtcatccg gggcggccgc gtgtcgatga
     4081 tttttctcat ctcccaaacg gccgcgtttc ccttccgcca ggaatgatcc gaggcggcga
     4141 tccggagaat gatttcgccg tcggtcaaac cggccggaag cggacggagg gaaatcgttt
     4201 tttccactaa ggcgacgggt tcgggaaatt cctcggatag aagctcaacc gttcgtccgg
     4261 cttggtccgc ctcgacccgg atccgggcca gcccgctctt gcggtcctcg aaccggaggg
     4321 ataattcccg gccgagcgcc ggcccgtcgc gaagcaggcg gaccgccggc ttctcggact
     4381 cgaacttgac gatccagagc caggcgccgg cggccaggac ggccggaatc agaagccagc
     4441 gggcgttttt gaatagattg gttttcggac ccgcgttctt ccagcgcatg atcatctccc
     4501 gttcgtgttt ttgaacggat caatattcca cacgctcctt cgttagtcaa ggaatttgga
     4561 actcgattac ttttcttgat tccgagaaat aaacaacttg acttttttca gcttccgcgg
     4621 tctataatat atccgctgta aaaatcacaa aggggaaaag cataaaagat cggtatcaga
     4681 ttgcctttag atcgtcgtca tgaaattgta tgcgcattcc aaagccgata cgccgcaaaa
     4741 tcaatggcat cccctggaag aacatcttca cgcgactgcg aataatgcgc gtcattttgc
     4801 agaaacttgg gaatccggag cgtgggctta caatgcgggt ctcttgcacg acttgggaaa
     4861 aagcgattcg agatttcaag gatatctcct ccgctgcaac gaactggacg attcagatta
     4921 tgactcgggc cgcgtcaatc attcctcaac aggagcctca tgcgccgaag acggcatcaa
     4981 gccgttcgga cgcatactcg cctatttaat agcggggcat cacgcgggat tgcccgattg
     5041 gttttcatcg gaatcggcaa atgccgccct ttcaatgcgt cttaaagaag gacgtgaaaa
     5101 ttcgaaacgg ataagagctt tctctgaaga gttgatttta tcacttctgc caatgacgaa
     5161 gccgtctttt gtgaacgcgg ccaacttcca cttctgggca cggatgttgt tctcatgctt
     5221 ggttgatgcg gattatctcg acaccgaaca gttcatggat gaagagaaat cccggcaacg
     5281 aagccattat ccttatctct cttctctcgc tcctcgttta ttcgattttt tagatgatct
     5341 ggaacagaag gcacctcaat catcggtaaa tgaaatccga gcggaaatcc gccgtgcctg
     5401 tgagcggaaa gccgattcgc cgaaaggctt attc
//
LOCUS       DAOOXH010000129         5388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_17658_length_5388_cov_8.531408, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000129 DAOOXH010000000
VERSION     DAOOXH010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17658_length_5388_cov_8.531408"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..981
                     /locus_tag="PKV64_10790"
     CDS             127..981
                     /locus_tag="PKV64_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPN17601.1"
                     /translation="MKIALAQYSPVLGDIPRNLDAHLELSAKARRAGADLLVFPELSL
                     TGYRLRDLVAETALDPRSSRTFASLREASRDVALVVGFVEESPLEKGLFYNSAAFLHK
                     GSLLHVYRKVFLPNSGMFEEMRFFAPGRTFRSFPTPWGRAGLLICRDFLHLNAHYLLF
                     ADGAEIILAVSAAPGRGVGEENGFTSCRMWEGIGETVSRVTTSFVVYCNRVGIEDGAV
                     FAGGSFVYDPFGRRVAQAPYFEPHLLLADLDPAAVRKARQALTLKRDDRPDITLANLE
                     RMIHRDAH"
     gene            971..1783
                     /locus_tag="PKV64_10795"
     CDS             971..1783
                     /locus_tag="PKV64_10795"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HPN17602.1"
                     /translation="MRINALFVEKILTGFIRGELAKRGFKKGILGLSGGLDSAVCAVL
                     AARALGPKNTIALIMPYGRGFGPDLEDARDTARRLKIDARTIDIAPMVDAYFKRHPSG
                     DLLLKGNKMARERMSILYDWSAREKALILGTSNKSELLIGYGTIHGDLACAVNPLGDL
                     YKTQVRELALHLGLPERIRTKAPTAGLWAGQTDEEEIGLTYDVLDEILELLVEGRETA
                     AEVAARGFPKKQVEKITGLIRRSEFKRQMPPIVKLSHRTIGHDFLYPLERGR"
     gene            1787..2491
                     /gene="rsmI"
                     /locus_tag="PKV64_10800"
     CDS             1787..2491
                     /gene="rsmI"
                     /locus_tag="PKV64_10800"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPN17603.1"
                     /translation="MIGTLYLVATPIGNLEDITFRAIRVLREADLVACEDTRQTLKLL
                     NKYDLRKSLISFYEYREKQRIPRLLDLLQSGKSVALVTDAGTPGVSDPGFRLVREAVR
                     LQIPVVPVPGPAAVIAALAASGLPTHRFLFMGFAPPKREALRKTLLSIREEEGTVIFY
                     LPARKIELFLETLPADFADRLVVIAREMTKIHEEFLRGTPAGLLEIAKRREFKGEVTI
                     LIHGAGRREAGSDRSG"
     gene            2702..3166
                     /locus_tag="PKV64_10805"
     CDS             2702..3166
                     /locus_tag="PKV64_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPN17604.1"
                     /translation="MAIIRWDPFRDMMTLREKMNRLFDDSLSQKGEERDMFTSNWSPA
                     VDIYETGNELVLTAEVPGIDEKDIEIKVEDNTLTLKGERKFEKETKEENFHRIERSYG
                     SFFRAFSLPNSIDADRIQAEHENGVLRVVMPKRQELKPKTVKILKPSEKSKK"
     gene            complement(3235..3972)
                     /locus_tag="PKV64_10810"
     CDS             complement(3235..3972)
                     /locus_tag="PKV64_10810"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPN17605.1"
                     /translation="MAQAPLFSKIGRLAELAFFPSFCKICGRLLEKPGERILCRDCLE
                     RIELSRSPKCPFCGKLYEGAGESHACGACAASPPSFSRHRSAGRYRGVLKDVLLLLKY
                     GKCRPLAPILGKAIHESLRRDGELWRDVDLIVPVPLYKKRRRERGFNQSADIARDIGR
                     RTGIPVAHKVLQKIRSTPPQTSLERRDRIENVRGAYAVGRKRAVEGRIVLLVDDVYTT
                     GSTLGECARVLRGAGAAEVRAVTVAQA"
     gene            complement(3975..4349)
                     /locus_tag="PKV64_10815"
     CDS             complement(3975..4349)
                     /locus_tag="PKV64_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HPN17606.1"
                     /translation="MPSKGSVVNKKELEAVKKTLETRRGEIIRKLSEAQNQSKIIETD
                     IAQDLADKAESSYTKEFLLSLSNAERGELLQIDAALKRIARGEYGLCLSCQKEIGKKR
                     LHHIPWTSFCIECQEKSEAETA"
     gene            complement(4339..4806)
                     /locus_tag="PKV64_10820"
     CDS             complement(4339..4806)
                     /locus_tag="PKV64_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPN17607.1"
                     /translation="MRIRILWPGRTKTAAVRALEDFYLKRLAGLLPCEIVVTEASRGL
                     DEKETEKILEIEARGLEKRLKDDYIVCLSDKGREMNSEDFGRFIEKRAVSGTKSLAFV
                     VGGFLGLAPRILDRADLRMSLSKMTLSHELCRAVLLEQIYRGASLMKGRSYAK"
     gene            complement(4837..5241)
                     /gene="rsfS"
                     /locus_tag="PKV64_10825"
     CDS             complement(4837..5241)
                     /gene="rsfS"
                     /locus_tag="PKV64_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPN17608.1"
                     /translation="MTKPKKTSAKAVAAAKTAKLTRRLLPPALKAAIEASLNKKAEDV
                     VVLDLRAAVTFTEFFLIMTGLNVRQTGALAEAIEHDLKAMNLRPIGMEGAARGEWILM
                     DYGWFIVHIFSPASRDYYALERLWSDASSLMV"
BASE COUNT         1101 a         1655 c         1498 g         1134 t
ORIGIN      
        1 cctgtctttt taccgaagta aacgtctttg aggtggagag cgatatggtt cactgggctt
       61 attttaacga aaacagggaa aatatcaaaa tttggcccgt ccacggattt atgctaattt
      121 gaagatatga aaatcgccct ggcccagtat tcgccggttt tgggagacat cccccggaac
      181 ctcgacgccc acctcgagct ctccgccaaa gcccgtcggg ccggggccga tctcctcgtt
      241 tttccggagc tgagtctgac cggctatcgg ctgcgcgatc tcgtcgccga aaccgccctg
      301 gaccctcgct cctcccggac cttcgcctcc ctgcgcgagg ccagccgcga tgtcgccctg
      361 gtcgtgggct tcgtcgaaga atcgcccctg gaaaaggggt tgttttataa ctccgccgcc
      421 tttcttcaca agggatccct cctccatgtc taccgaaagg ttttcctgcc caattccggc
      481 atgttcgaag aaatgcgctt tttcgctccg ggacggacct tccgctcctt tccaacgccc
      541 tggggccggg cgggccttct gatctgccgc gatttcctcc atctcaacgc gcattatctc
      601 ctgttcgccg acggagccga gatcattctc gccgtcagcg ccgcccctgg ccggggcgtc
      661 ggcgaagaga acggattcac ctcctgccgg atgtgggaag ggatcgggga aaccgtctcc
      721 cgggtcacca cctccttcgt cgtttattgc aaccgggtcg ggatcgagga cggagcggtc
      781 ttcgccggag gttcattcgt ctacgatcct ttcggccggc gcgtcgccca ggccccctat
      841 ttcgagccgc atcttctcct ggctgatctc gatcccgccg ccgtccgcaa ggcccgccaa
      901 gccctgaccc tcaaacgcga cgaccgtccg gacatcacct tggccaacct cgaaaggatg
      961 atccatcgcg atgcgcatta acgccctctt cgtcgaaaag atcctgaccg gctttatccg
     1021 cggggagctg gccaagcgcg gcttcaaaaa agggattctc ggcttgtcgg gggggctgga
     1081 ttcggccgtc tgcgccgtct tggccgcccg ggcgctcggc ccgaaaaata cgatcgcctt
     1141 gatcatgccc tacggccgcg gattcggccc ggatcttgag gacgcccggg acacggcccg
     1201 gcgattgaaa atcgacgccc gaacgatcga catcgccccg atggtcgacg cctacttcaa
     1261 gagacacccg agcggcgacc ttctcctcaa gggaaataag atggcccgcg agcggatgtc
     1321 catcctctac gactggtcgg cccgggaaaa ggcgcttatc ctgggaacga gcaacaagag
     1381 cgagcttctc atcggctacg ggacgatcca cggcgactta gcctgcgccg tcaatccgtt
     1441 gggcgacttg tataagactc aggttcgaga gctggccctt cacctgggcc tgccggagcg
     1501 catccggacg aaggcgccga cggccgggct ttgggccggt cagacggacg aggaggaaat
     1561 cggcctgacg tacgatgtcc tcgacgagat tctcgaactc ttggtcgaag gccgggaaac
     1621 cgcggccgaa gtcgcggccc gcggcttccc caaaaagcag gttgagaaaa tcaccggcct
     1681 catccgccgg tcggagttca aaaggcagat gcccccgatc gtcaagcttt cccaccggac
     1741 gatcggacac gattttcttt atccccttga acggggccgt tgagccgtga tcgggacgct
     1801 ctatctcgtc gccacgccaa tcggaaacct ggaagacatc acgtttcggg cgatccgcgt
     1861 cctccgggaa gcggatctcg tcgcctgcga agacacccgc cagaccctca agctgctcaa
     1921 caagtacgac ctgaggaaaa gcctgatcag cttctacgag taccgggaaa agcagcggat
     1981 tccccgcttg ctcgatcttc ttcaatccgg aaagtcggtc gccctggtga cggacgccgg
     2041 cacgccggga gtttccgatc caggtttccg ccttgtccgg gaggcggtcc gcctccaaat
     2101 ccccgtcgtt cctgttcccg gcccggcggc cgtgatcgcg gctttggccg cgtccggcct
     2161 gccgacgcac cggtttcttt tcatgggctt cgcgcctccc aagcgggaag ccttgcgtaa
     2221 aacgctcctg tcgatccggg aagaagaagg aacggtcatc ttctatctcc cggcccggaa
     2281 aatcgagctt tttctagaaa cccttccggc ggatttcgcg gaccgtcttg tcgtcatcgc
     2341 ccgggaaatg acgaagatcc acgaagaatt cctccgggga accccggccg ggctccttga
     2401 aatcgccaaa aggcgcgaat tcaaaggaga agttacgatt ctcatccacg gagccggaag
     2461 aagggaagcc ggttcggatc gttccggctg aagctggaat tttcgaattt ttttcttttt
     2521 taaagaatat ttccgaacag cagaaacata gagactttat tttattaatt atcaattatt
     2581 tataatgtaa ataataatta aataaatttt ttaatattac ttgacaatat accgcttaga
     2641 tattatattg gacttggccg cttataaggc cgataacgaa aaattaatca ggaggttcaa
     2701 tatggctatc atcagatggg atccctttcg cgacatgatg acgctgaggg aaaaaatgaa
     2761 ccggcttttc gacgactctc tgtcccaaaa gggagaggag agagacatgt tcacgagcaa
     2821 ctggtcgccg gccgtcgata tctatgagac cggcaacgag ctcgttctga ccgcggaagt
     2881 gcccggcatc gacgagaaag acatcgaaat caaggtcgag gacaacaccc tgaccctcaa
     2941 gggcgaacgc aagttcgaga aggagaccaa ggaggagaac ttccaccgga ttgaaagatc
     3001 ctacggctcg ttcttccggg ctttctccct gcccaactcg atcgatgccg accgcattca
     3061 agccgagcac gaaaacggcg ttctccgggt ggtcatgccc aaacggcagg aactcaagcc
     3121 gaagacggtc aagattctca agccttctga aaaatcgaaa aagtaacgac ggatcccctc
     3181 ctccgccgta aaaatgacgg ggcgaaagcc ccgtcatttt ttttctccgc cgtttcaagc
     3241 ctgggcgacg gtcacggccc tcacctcggc cgcccccgct cctctgagga cgcgggcgca
     3301 ttcccccagc gtcgagcccg tcgtgtagac atcgtcgacc aggagaacga ttctcccctc
     3361 gaccgcgcgt ttccggccga cggcataggc gccgcggaca ttctcaatcc ggtcgcggcg
     3421 ctccagggac gtctgcggcg gcgtgctccg gatcttttgg aggaccttgt gggcgaccgg
     3481 gattccggtc cgccgcccga tgtcccgggc gatatccgcg gattgattga atccccgctc
     3541 ccggcggcgt ttcttataaa gcggaaccgg aacgatcaga tcgacatccc gccacagctc
     3601 cccgtccctc cggagggatt cgtggatcgc ttttccgagg atgggggcca gcggccggca
     3661 tttcccgtac ttcaggagta aaaggacatc cttgagaacg ccccggtagc ggccggccga
     3721 acggtgccgg ctgaaagacg gcggggacgc cgcgcaggcg ccgcaggcat ggctttctcc
     3781 ggccccctcg tacagctttc cgcaaaaggg acatttcgga gaacgggaaa gctcgatccg
     3841 ctccagacaa tcccggcaaa ggatccgctc gccgggcttt tccaaaagcc ggccgcatat
     3901 tttgcagaac gaaggaaaga acgcgagctc ggccagtcgg ccgattttcg aaaaaagcgg
     3961 cgcctgggcc aaaatcaggc tgtttcagct tcgctctttt cctggcactc gatgcagaag
     4021 gaggtccaag gaatatgatg gaggcgtttt ttgccgatct ccttctggca gctcaggcag
     4081 agcccgtatt cgccccgggc gattcttttg agggcggcat cgatctgaag gagctccccg
     4141 cgctcggcgt tggacaggct gaggaggaat tccttcgtat aggagctttc ggccttgtcg
     4201 gccagatcct gggcgatgtc ggtttcgatg attttactct ggttttgggc ttcgctaagc
     4261 ttcctgatga tttcgccccg ccgcgtttca agggttttct tgacggcttc cagctctttc
     4321 ttgttcacga cgctcccttt acttggcata agatcttcct ttcatgagtg atgcgccgcg
     4381 gtagatctgc tccagcaaaa ccgcgcggca gagctcgtgg gacagggtca tcttggacag
     4441 cgacatccgg agatcggccc ggtccaggat ccggggcgcc aaacccagaa acccgcccac
     4501 gacaaaggcc agcgatttcg ttccagagac cgctcgtttc tcgatgaaac ggccgaaatc
     4561 ttcggaattc atctctctcc ctttgtcgga taggcagaca atataatcat ctttaagccg
     4621 tttttcaagc ccccgggcct caatttccaa gattttttcg gtttccttct cgtccaggcc
     4681 ccgggaagcc tcggtgacga cgatttcgca gggcaacaaa ccggcgagac gcttcagata
     4741 aaagtcctcc agcgcgcgga ccgccgccgt tttcgtcctt cccggccaaa gaatccggat
     4801 ccgcatccct tttgcctctc cccggcgaaa gacgcctcag accatcaagc tggacgcatc
     4861 gctccagagc cgctccaggg cataatagtc ccgggaagcg ggggagaaga tatgaacgat
     4921 gaaccacccg taatccatca ggatccattc tccccgggcc gcgccctcca tcccgatcgg
     4981 ccgaagattc atggccttga ggtcgtgttc gatcgcctcg gccagggccc ccgtttgacg
     5041 gacgttcagc ccggtcatga tcaggaaaaa ttcagtgaac gtgaccgcgg ccctgaggtc
     5101 gaggacgacg acgtcctccg ctttcttgtt taaggacgct tcgatcgccg ctttcagggc
     5161 gggcggcagc agccgccgcg ttaattttgc ggttttcgcc gccgcgacgg ctttggctga
     5221 tgtttttttc ggtttggtca tttttttgtc cccgatagag attgccggag cggataaggg
     5281 cggccactcc ggccggaacc aggctctcga tcggccgtcc ttctccgacc cgccgacgga
     5341 tgtccgagga ggataccgcc agggcctgga tcctaaggaa aaaaatcg
//
LOCUS       DAOOXH010000130         5205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_18505_length_5205_cov_4.635728, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000130 DAOOXH010000000
VERSION     DAOOXH010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18505_length_5205_cov_4.635728"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PKV64_10830"
     CDS             complement(<1..297)
                     /locus_tag="PKV64_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17609.1"
                     /translation="MKKQTASLALFVLGLNIGFLFAPAGFACDTWVALRDAVSRGRVL
                     FAKNSDRTVFDSQPLALHPRKSWPAGAMIDLGRVSIPQAGETFATLGSGPYWCWG"
     gene            complement(321..1010)
                     /locus_tag="PKV64_10835"
     CDS             complement(321..1010)
                     /locus_tag="PKV64_10835"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HPN17610.1"
                     /translation="MLKLVGKDLLAARWILLCGAAVLSLYSLQPFFSAGFILAAGGAL
                     ALAGQATVFFLEDRNKTEILYLSLPVSRASIVGARYLLGAVLLAAGGAMVFGIIGWAG
                     ALMHVPRPAGGFPGLLSIEAAALFFPLTAFFLSLYLPFYHRFGFGRGTLLFTAAGVLC
                     AALGAAGLKAALSDAFASGGGEAVIFGAVRVLRLSLGTPLFVLAAAAAVFIPFLVSLF
                     LSTRFYARREF"
     gene            complement(1003..1920)
                     /locus_tag="PKV64_10840"
     CDS             complement(1003..1920)
                     /locus_tag="PKV64_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN17611.1"
                     /translation="MDCALEIKDLSKSYSPSGRLTRPAPDSAGSGGGFHLNEVSFSLP
                     RGYIMGLIGPNGAGKTTIIKLILNLIRRDGGEIRVLGLDNIAGEMEIKSRLGFVHDTP
                     FFYEHLTLADVRKTVAPFYRDWDDACFRRLAEEFELPLGKRFKTLSRGMKMKFALAAA
                     LSHKAELLILDEPTSGLDPVFRRELLRKLSAFIQDEGKSVLFSTHITSDLERIADFIT
                     FIRGGRIVFSSPRDRILGAWVVVKGGPELLAAENRPLFKGIRQSACGVHALTAEADEA
                     RRRFGREAIVETATLEDIMFFMSKGNGHA"
     gene            complement(1930..2601)
                     /locus_tag="PKV64_10845"
     CDS             complement(1930..2601)
                     /locus_tag="PKV64_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HPN17612.1"
                     /translation="MIKLFRKDFILHKKFVLGVGLVYPLYIGYMGSRIGRTTLLPVLC
                     AFLYVIVPFVLFGREDKFKAETFTLSLPVTRREYLRARFLLSWILMLLMFAAGSLLMA
                     VVPGAKLPAATVFGPRMVLSSLAFIAVYFGLLMPLFVRFGQAGLLVFLVVLQVTGVLL
                     MVFRSSVGIGLIKTLFRLVPNAVAEIQAAFGPAPAVFAVLSLLALFSYGSFSLSLALF
                     RRKEY"
     gene            complement(2594..3457)
                     /locus_tag="PKV64_10850"
     CDS             complement(2594..3457)
                     /locus_tag="PKV64_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN17613.1"
                     /translation="MAAALEISGLRKSYKGFALKDVSFAVPEGSIMGLVGPNGAGKTT
                     IVKLILNLVRRQAGAIKVFGLDNLADEKAVKNRIGFVHETPALVEDVPLRDIASVVAP
                     FYPCWNPALFQAMLREFELPAGKKFKALSHGMKMKFSLALALSHDADLLILDEPTSGL
                     DPVFRRELLDRLAGVIQDGRKAVLFSTHVTSDLERTADYVTLLLDGEVVLSAPRDDIR
                     DKWAVVKGGPELLDPVRCPLFRAVRESPYGVEALTGDIPASRAAFPSAVFDRASIEDI
                     MVFLHSGASHD"
     gene            complement(3457..3834)
                     /locus_tag="PKV64_10855"
     CDS             complement(3457..3834)
                     /locus_tag="PKV64_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPN17614.1"
                     /translation="MKIIISNSSPDPIYEQIGKQIKAQILSGDLEEGSPLPSIRRLAQ
                     DLQISVITTKRAYDELEAEGFLDTVGGKGTFVAVQNKELLREKKMKVVEEALARAVDE
                     ARLMGIEESQVADMLRLLFKENI"
     gene            3982..4755
                     /locus_tag="PKV64_10860"
     CDS             3982..4755
                     /locus_tag="PKV64_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17615.1"
                     /translation="MKNAVIASLFLVPALLLTFFPAGCSGFSSDSEAALEAARVAILE
                     EFNRVNIELRRAAERLGASGLTGCDARSALEDLYLAIPSAVDCAAVDSRGIMVTIEPA
                     SYRHHENKDISGQEQVKRMLDRREPVMTGIFLTVEGIEAGDAEYPVFGPEGAFLGSVS
                     LLFSPEKLLDGALSPVSGKTDGLVVIETGGRILRHSDGARVGTNLFASAGFKSDERMR
                     RLGERLAAELEGRDGPTGASWKSVELYNGFWRIVDLPSR"
     gene            complement(4763..>5205)
                     /locus_tag="PKV64_10865"
     CDS             complement(4763..>5205)
                     /locus_tag="PKV64_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPN17616.1"
                     /translation="PGQTIEQAHMRSRALVSQWVYEKGMPENVISRIEKDGKTYFVIN
                     DYRKLRTLYGRLLAEIQRITSEGDYEAAKNLVEAYGVKIDPELHAEVLARYAKLNLAP
                     YAGFINPVYTPVTDGSGKIVDVKISLPEDYVAQMLEYGKKYGFL"
BASE COUNT         1117 a         1555 c         1634 g          899 t
ORIGIN      
        1 tccccagcac caatacgggc cggagccgag ggtggcgaat gtctccccgg cctgggggat
       61 ggaaacgcgg ccgaggtcga tcatcgcgcc ggccggccat gatttccgcg gatgaagggc
      121 cagcggctgg ctgtcgaaaa ccgtccgatc gctgtttttc gcaaacagca cccgcccccg
      181 ggacacggcg tccctgaggg ccacccaagt gtcgcaggcg aaccccgccg gggcgaagag
      241 aaagccgata ttcaggccca aaacgaaaag agcgagggaa gcagtctgtt ttttcatcgg
      301 ataccccccg gccgccggga ttaaaactcc cggcgggcgt aaaacctcgt cgaaagaaag
      361 agggacacga gaaaggggat gaagacagcc gccgcggcgg ccaagacaaa aaggggcgtc
      421 cccaaggaga gccggagaac ccggacggcc ccgaaaataa cggcctcccc gccgcccgaa
      481 gcgaaggcgt ccgagagggc ggccttgagc ccggccgcgc ccagcgcggc gcaaaggacg
      541 ccggcggccg tgaacaggag cgttccgcgg ccgaagccga agcggtgata gaacggaagg
      601 tagagagaca ggaaaaacgc ggtcagcgga aaaaacaagg ccgccgcttc gatcgaaaga
      661 agcccgggga acccgccggc cgggcgcgga acgtgcatca aggcgcccgc ccagccgatg
      721 atcccgaaga ccatcgcgcc gccggcggcc agcaggacgg ctcccagaag ataacgggcg
      781 ccgacgatcg acgctctcga gacagggagg ctgagataga gaatttcggt tttgttccga
      841 tcctcgagga aaaagaccgt cgcctggccg gccagagcaa gcgcaccgcc ggccgccagg
      901 atgaatccgg ccgaaaagaa cggctgaagc gagtaaagag ataggacagc cgctccacag
      961 agcagaatcc accgggccgc gagcagatct ttcccgacga gcttaagcat ggccgtttcc
     1021 cttgctcatg aaaaacatga tgtcctcgag ggtggccgtt tcgacgatcg cctcgcggcc
     1081 gaaccgccgg cgggcttcgt cggcctcggc cgtcagggca tggacgccgc aggcgctctg
     1141 ccgaatcccc ttgaacaagg gccggttctc ggcggccagg agctccggcc cgcccttgac
     1201 cacaacccag gccccgagaa tccggtcccg gggcgaggag aaaacgatcc ggccgccgcg
     1261 gatgaaagtg atgaaatcgg caatccgctc gaggtccgaa gtgatatggg tggagaagag
     1321 gaccgatttg ccttcatcct ggataaaagc cgatagcttt cttaaaagtt cgcgccggaa
     1381 gacgggatcc aggcccgagg tcggctcgtc gaggatcagc agttcggcct tgtgggacaa
     1441 ggcggcggcc agggcgaact tcattttcat cccccgcgac agggtcttga atctttttcc
     1501 cagaggcagc tcgaactctt ccgccagccg ccggaaacaa gcgtcgtccc agtcccggta
     1561 aaacggggcg actgttttcc ggacatcagc cagggtcaag tgttcgtaga aaaacggcgt
     1621 gtcgtggacg aatcccaacc ggcttttgat ctccatttct ccggcgatat tgtccaggcc
     1681 aaggactcgg atttcgccgc cgtcccggcg gatgaggttc aggatgagct tgatgatcgt
     1741 cgttttaccc gccccgttgg ggccgatcaa gcccatgatg tacccccggg gcagggagaa
     1801 ggagacctcg ttcaaatgaa acccgccgcc cgacccggcg gaatcgggcg ccggccgggt
     1861 caggcggccg gacgggctgt aagacttgga aaggtccttg atttccaggg cgcagtccat
     1921 ggcccctcct taatattcct tcctccggaa caaggccagg ctgagggaga acgagccgta
     1981 ggaaaacagg gccaggaggg agagaaccgc gaagacggcg ggcgccgggc cgaaagcggc
     2041 ttgaatttcg gccaccgcgt taggaaccag gcgaaagagc gtcttgatca agccgatgcc
     2101 gacggagctg cggaaaacca tgagaaggac gccggtgacc tgcagaacga cgagaaaaac
     2161 gagcagcccg gcctggccga aacggacgaa caaaggcatc aacaaaccga aatagacggc
     2221 aatgaaggcc agggaggaaa gaaccatgcg aggcccgaaa accgtcgcgg ccggaagctt
     2281 cgctcccgga accacggcca tcaagaggga gccggcggcg aacatgagga gcatcaatat
     2341 ccagctcaga agaaaccggg cccggaggta ttcccggcgg gtgacgggaa gactgagagt
     2401 gaacgtttcg gctttgaact tgtcctcccg gccgaataga acgaagggga cgatgacgta
     2461 gaggaacgcg caaaggacgg ggaggagcgt cgtgcggccg atccgggagc ccatataacc
     2521 gatatagagc ggatagacga gtccgactcc caagacgaat ttcttatgga gaatgaaatc
     2581 cttgcggaac agtttaatca tgggaagctc cggaatgcag gaagaccatg atgtcttcaa
     2641 tcgaggcccg gtcgaagacg gccgagggga aggcggcccg ggacgctgga atatcccccg
     2701 tcaaggcctc caccccgtag ggcgattccc ggacggcccg gaacagggga caacggacgg
     2761 gatcaaggag ctcgggcccg cccttgacca cggcccattt atcccggatg tcatcccgcg
     2821 gcgccgagag aacaacttct ccatcgagca gcagggtcac ataatcggcc gtcctctcca
     2881 aatccgaggt gacgtgggtg gaaaaaagaa ccgccttccg gccgtcttgg atcacgccgg
     2941 cgagacggtc cagaagctcc cgccggaaga cggggtccag gccggaggtc ggctcgtcga
     3001 gaatcagaag gtcggcgtcg tggctgaggg ccagagccaa ggaaaatttc atcttcatcc
     3061 catgggacag ggccttgaat ttctttccgg ccggaagctc gaactcccgg agcatcgctt
     3121 ggaacagggc gggattccag catggataga aaggcgccac gacggaggcg atatcccgaa
     3181 gcgggacgtc ctcgaccagg gccggcgtct cgtggacgaa cccgatccgg ttcttaactg
     3241 ctttttcatc ggcgaggtta tccaggccga agaccttgat cgcccccgcc tgacgccgaa
     3301 ccaagttcag gataagcttg acgatcgtcg ttttgccggc gccgttggga ccgaccagcc
     3361 ccatgatcga gccctcgggg acggcgaacg acacgtcctt gagggcgaat cccttgtagg
     3421 acttgcggag tccggagatt tcgagggcgg cggccatcag atattctcct tgaacagaag
     3481 acggagcatg tccgccacct ggctctcttc gatacccatc agccgggcct cgtcgacggc
     3541 ccgggccagc gcttcctcga cgactttcat cttcttctcc cggagaagtt ccttattctg
     3601 gacggcgacg aaggtgccct tgccccccac cgtgtcaagg aagccctcgg cttcaagctc
     3661 gtcgtaagcc cgcttggtcg tgatcacgct gatttggagg tcttgggcaa ggcgccggat
     3721 ggaaggcaac gggcttccct cctccaagtc gccggagagg atctgggcct tgatctgttt
     3781 tccaatctgc tcgtagatgg ggtcgggcga ggagttggaa atgatgattt tcaaaaccgg
     3841 ggctccttcc gttctacttg tatcatttgt ttatactgtt tatatattac aatcacagta
     3901 tagccggcgg tttttatttg tcaagtattt tttataaaat aagaaggcgt ttcaatccgc
     3961 ctggaatcgg aggctctgac catgaaaaat gccgtaatcg cttcactttt cctcgttccc
     4021 gcccttctcc tgactttctt tcccgccgga tgttccggtt tctcttccga ttccgaggcg
     4081 gcgctggaag cggcccgggt agcgatcttg gaggaattca accgggttaa tatcgagctg
     4141 cgccgggccg ccgagcgcct gggagcgtcg ggcttgaccg gatgcgacgc ccgctcagcc
     4201 ctcgaggatc tttatctcgc catcccctcg gccgtggatt gcgccgccgt cgactcccgc
     4261 gggatcatgg tgacgatcga gccggcgtcc taccgtcacc acgaaaacaa ggacatcagc
     4321 ggccaggagc aggtgaagag gatgctggat cgccgcgaac cggtcatgac cggaatcttc
     4381 ctgactgtcg aggggatcga ggccggcgac gccgaatacc cggtcttcgg gcccgagggg
     4441 gcgttcctcg gctccgtcag cctgcttttt tcgccggaga agctgctgga cggcgctctc
     4501 tcccccgtct ccggaaagac cgacggcctt gtcgtgatcg agacgggcgg acgcatcctc
     4561 cgtcattcgg atggcgcccg cgtcggaaca aaccttttcg cctcggccgg cttcaagagc
     4621 gacgaacgca tgcgccggct tggggagcga ctggccgccg agctcgaggg acgggacggc
     4681 ccgacgggcg catcctggaa aagcgtcgag ctttacaacg gcttctggcg gatcgtcgac
     4741 ctgccgtctc gctgatattc aatcacagga agccgtactt ctttccgtac tccagcatct
     4801 gggcgacgta atcctcgggc agcgagatct taacgtcgac gatcttgccg cttccgtccg
     4861 tgacgggcgt atagacggga ttgatgaagc cggcgtaggg agccaagttc agcttggcgt
     4921 accgggccag gacctcggcg tggagttccg gatcgatctt gacgccgtag gcctcgacaa
     4981 gattcttggc ggcctcgtaa tcgccctcgc tcgtgatccg ttggatctcg gccaaaagcc
     5041 ggccgtagag cgtccgcagc ttccggtaat cgttgatcac gaaatacgtc ttaccgtcct
     5101 tttcgatccg gctgatgacg ttttccggca tccctttctc atagacccat tgggagacca
     5161 gcgcccggct ccgcatgtgg gcctgctcga tcgtctggcc cggct
//
LOCUS       DAOOXH010000131         5193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_18557_length_5193_cov_6.167964, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000131 DAOOXH010000000
VERSION     DAOOXH010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18557_length_5193_cov_6.167964"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PKV64_10870"
     CDS             complement(<1..433)
                     /locus_tag="PKV64_10870"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HPN17617.1"
                     /translation="MKRAVFAVLALLLPLSGFAQDMIERIDISGNDRVTRDTIMYYLS
                     IREGDVYNLAMLRKDFRVLWSTGFFSNITFDEDQGARGKIVKIAIEENPVIRMITYKT
                     GRKVKENDITAKLKEKDEVLLPYSSYSPAKLQRVKKTIEDLL"
     gene            complement(500..1183)
                     /locus_tag="PKV64_10875"
     CDS             complement(500..1183)
                     /locus_tag="PKV64_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN17618.1"
                     /translation="MSEILRTVDLAKDYLLPGGEILHVFSGLNFSLDRGEIVAVMGAS
                     GSGKTTFLNLLGALDRPTAGKVFLDGEDLSAKNAVSLARIRNVKIGFVFQSFHLLPEF
                     TAAENVALPMLMSGRGRAEAFRRAAEVLGEVGLAGKARCRPAQLSGGEMQRVAIARAL
                     VNDPAVLLADEPTGNLDWKTGEAILRLLLDLHEKRGLASVLVTHNEKVSILCEKIFFL
                     ERGALERAV"
     gene            complement(1176..2396)
                     /locus_tag="PKV64_10880"
     CDS             complement(1176..2396)
                     /locus_tag="PKV64_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPN17619.1"
                     /translation="MGYERFIARRYLTARRKQAFISVITVISTVGVAIGVAALIIAVA
                     LITGFQKDVQDKILGATSHLMVSEVTGAGLADYEAAAAKIRPRKGVTSVSPVAYGMVL
                     LQGGKAQGAMLKGMDLVQERKIQPWLVSLEAGTMPGEGIGTREGILLGRDLAFAVGAG
                     VGDVITAVTTSSRLGPLGLTPRTKLFQVTGIFRTGLYEFDSTTALTGLAAAQKFFGLD
                     GRISYLQVMISDIFAADRMKDELKGLLPPLTYITTWTELNQSLFSALKLEKNIIFLTI
                     TLIVIVAALNIIATLILMVMEKTRDIGILMALGATPAEIRRIFFLQGAMIGIAGTAVG
                     TALGLLWCLAANAFELIRVPVDIYQISYVPFRIMPLDLFLIIGTALSISLVSTLFPSH
                     RAAKVNPVTALKYE"
     gene            complement(2406..3974)
                     /gene="lysS"
                     /locus_tag="PKV64_10885"
     CDS             complement(2406..3974)
                     /gene="lysS"
                     /locus_tag="PKV64_10885"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPN17620.1"
                     /translation="MTDPQNPKPLPGPPEPPAEADGGPRPADELRRSEQELVRADKYR
                     RLIAAGVDPFPAKAGRTHSVTELVEAYAARSAEEVEAAAVSVVVPGRMMTVRLMGKAL
                     FFHISDGRSKIQVYLRTDKVGPEAYELFKLIDIGDILTVSGTLFKTRTGELTVLCSSY
                     VFLAKCFHPLPEKWHGLQDAEIRQRRRYLDLIMNPEVGEVFRLRSAIISWLRKFFDGR
                     GYIEVETPMMQPLAGGALARPFVTHHNALDLDLYLRIAPELYLKRLVVGGMEKVYEIN
                     RNFRNEGIDSEHNPEFTMLEFYEAYRDYHDMMDLTEEILCGLCADLLGKDEFPYGDQV
                     ISLKRPFRRLRFREALAEGSGLPAEAFDDRPRIIAYAEGLAPEKKPLTYGRALDVIFD
                     KRVKESLVQPTFIINPPKDVSPLAKSAPGAPEEAARFELMIAGMELANAFSELTDPAE
                     QKARFEQQAEERMKGDEETHPIDLDYVQALEYGLPPTGGEGIGIDRLVMLLADRRSIR
                     DVILFPLLRPREEK"
     gene            complement(3889..4416)
                     /gene="nusB"
                     /locus_tag="PKV64_10890"
     CDS             complement(3889..4416)
                     /gene="nusB"
                     /locus_tag="PKV64_10890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPN17621.1"
                     /translation="MGRRRGARETALQILYELEFNESGPEEVLRRRKAEKPAGAGAGE
                     YAAWLVRGVFGRRSELDDRIQGAARNWRVARMGLVDRNILRLAVYEMLEEKTLVPAII
                     INEAVEIARRFSGGESAVFVNGVLDAVRRALSGEAGANHTSEKNEHDRSPKPQTPPRP
                     AGTPRGGGRRTPSGR"
     gene            complement(4420..4887)
                     /gene="ribH"
                     /locus_tag="PKV64_10895"
     CDS             complement(4420..4887)
                     /gene="ribH"
                     /locus_tag="PKV64_10895"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPN17622.1"
                     /translation="MKIHEGKLQAKGLKIGIVVSRFNSFLTDKLLDGALDALRKLGAE
                     EADITVCKVPGSFEAPLVVKKLAASGRVDGLVCLGALIRGETPHFDFLAAEVTKSLSQ
                     ISLETGVPVTMGVLTVDSIEQGIERSGTKAGNKGWDAVGSLVELLTLIKAAKL"
BASE COUNT          924 a         1634 c         1673 g          962 t
ORIGIN      
        1 ccagcaggtc ctcgatcgtt ttcttcaccc gctgcagctt ggccgggctg taggaggaat
       61 acggcagcag gacctcgtcc ttttccttga gcttggccgt gatgtcgttt tccttgacct
      121 ttctcccggt tttataggtg atcatccgga tcaccggatt ttcctcgatg gcgatcttga
      181 cgattttgcc gcgggcgccc tgatcctcgt cgaacgtgat gttggaaaaa aagccggtcg
      241 accagaggac acggaaatcc ttccggagca tcgccaagtt gtagacgtcc ccttcccgga
      301 tggacaggta atacatgatc gtgtcccggg tgacccggtc gtttccggag atatcgattc
      361 gctcgatcat gtcctgggcg aatccggaga gcggcaggag gagggcgagg acggcgaaaa
      421 cggctctttt catgttcata tccttggact tataccataa ggatcggttt tctttcaacc
      481 gaagattaga acgacgggat cagaccgccc gttccaacgc cccccgttcc agaaagaaga
      541 ttttttcaca aagaatcgag actttctcgt tatgggtgac gagaacggag gccaaacctc
      601 gtttctcgtg aagatccaga agaaggcgga ggatcgcctc gccggttttc cagtccaggt
      661 tgccggtcgg ctcgtcggcc agaaggacgg ccgggtcgtt gaccagggcc cgggcgatgg
      721 ccacccgctg catctcgccg ccggaaagct gggccgggcg gcaccgggcc ttgcccgcca
      781 gccccacctc tcccagcacc tcggccgccc ggcggaaggc ctccgcccgg ccgcggccgc
      841 tcatgagcat cggcagggcg acgttttccg cggccgtgaa ttccgggagg agatgaaagg
      901 attgaaaaac gaacccgatt ttcacgttcc ggatccgggc cagcgaaacc gcgttcttcg
      961 ccgacaggtc ttccccgtcg aggaaaacct tcccggcggt cggccgatcc agggcgccga
     1021 gaagattgag aaacgtcgtt ttccccgatc ccgaggcccc catcacagcc acgatttcgc
     1081 cccggtccag ggagaaattc aagccggaaa agacatgaag aatctccccg ccgggaagga
     1141 ggtaatcctt cgccaggtcg acggtccgca ggatctcact catatttcaa ggccgtgacc
     1201 gggttgactt tagccgcccg gtgagagggg aaaagcgtgg agaccaggct gatggacagg
     1261 gccgttccga tgatgaggaa caagtccaag ggcatgatcc ggaaggggac gtaggagatc
     1321 tggtagatgt ccaccggcac ccggatcagc tcgaaggcgt tggccgccag gcaccagagg
     1381 agccccaggg cggtcccgac ggccgttccc gcgatcccga tcatcgcccc ctggaggaaa
     1441 aagatcctcc ggatctcggc cggcgtcgcc cccaaggcca tcaggatgcc gatgtcccgg
     1501 gtcttctcca tgaccatcag gatcaaggtg gcgatgatgt tgagggcggc cacgatgacg
     1561 atcagggtga tggtcaggaa aatgatgttt ttctcgagct tgagggccga aaacagcgat
     1621 tgattgagct cggtccaagt cgtgatgtac gtcaaggggg gcaggaggcc cttcagctcg
     1681 tccttcatcc ggtcggcggc gaagatgtcg ctgatcatga cctgaaggta gctgatccgg
     1741 ccgtcgaggc cgaagaactt ctgggccgcg gccaggccgg tcagggcggt cgtcgaatcg
     1801 aattcgtaca agcccgtccg gaagatgccc gtgacctgga acagcttcgt cctgggagtc
     1861 aggccgaggg gtccgagccg ggaggaagtc gtcaccgccg tgatcacgtc gcccacgccc
     1921 gccccgacgg cgaaggccag atcccggccg agaaggatgc cctcgcgcgt tccgatcccc
     1981 tctccgggca tcgtcccggc ctccagcgag accagccacg gctggatctt ccgttcctgg
     2041 acgagatcca tgcccttgag catcgccccc tgggcttttc ccccctggag gaggaccatc
     2101 ccgtaggcca ccggcgaaac ggaggtcacg cccttgcgcg ggcggatttt cgcggccgcg
     2161 gcctcgtagt ccgccaagcc cgcccccgtc acttccgaaa ccatcaagtg ggacgtcgcc
     2221 cccaggatct tgtcctggac atctttctga aagccggtga tcaaggcgac ggcgatgatc
     2281 agggcggcca ccccgatggc cacgccgacg gttgagatga ccgtgatcac cgagatgaag
     2341 gcctgcttcc ggcgggcggt cagatagcgc cgggcgatga agcgctcata tcccatgatc
     2401 cgggcctatt tctcctcgcg ggggcgcaga agcgggaaca ggatgacgtc gcggatcgag
     2461 cgccgatcgg ccagcagcat caccaggcgg tcgatcccga tgccttcgcc gccggtcggc
     2521 ggcaggccgt attccagggc ttggacatag tcgaggtcga tcgggtgggt ttcctcgtcg
     2581 cccttcatcc gctcctcggc ctgctgttcg aaccgggcct tctgctcggc cggatcggtc
     2641 aactcggaga aggcgttggc cagctccatg ccggcgatca tcagctcgaa gcgggccgct
     2701 tcctcgggcg ctccgggggc cgacttggcc aggggcgaga cgtccttggg cggattgatg
     2761 ataaacgtcg gctggacgag ggactccttc acccgcttgt cgaagatgac gtccagggcc
     2821 cgcccgtaag tcagcggctt cttctccggg gccaggccct cggcgtaggc gatgatccgg
     2881 ggccggtcgt cgaaggcctc ggccggcagg ccgctgccct cggccagggc ctcgcggaac
     2941 ctgagccggc ggaacggccg cttcagggag atgacctgat cgccgtaggg aaactcgtct
     3001 ttgcccagga ggtcggcgca gaggccgcag agaatctcct cggtcaggtc catcatgtcg
     3061 tgatagtccc ggtaagcttc gtagaactcg agcatcgtga actcgggatt gtgctcggag
     3121 tcgatcccct cgttgcggaa attgcggttg atctcgtaaa ccttttccat cccgccgacg
     3181 accaggcgct tgagatagag ctccggggcg atccgaaggt acaggtcgag gtcgagggcg
     3241 ttatggtggg tgacgaacgg ccgggccagg gccccgccgg ccagcggctg catcatcggc
     3301 gtctcgacct cgatgtagcc gcggccgtcg aaaaacttcc ggagccagga gatgatcgcg
     3361 ctccggaggc ggaaaacctc tcccacctcg ggattcatga tcaggtcgag atagcgccgc
     3421 cgctggcgga tctcggcgtc ctggaggccg tgccatttct ccggcagagg gtgaaaacac
     3481 ttggccagaa aaacgtacga tgagcagagg acggtcaatt ccccggtccg ggttttgaac
     3541 agcgtcccgg acacggtaag aatgtcgccg atgtcgatca gcttaaagag ctcgtaggcc
     3601 tcggggccga ccttgtcggt ccggaggtaa acctggattt tagaccggcc gtccgaaatg
     3661 tggaaaaaca gcgccttccc catcaggcga acggtcatca tccgcccggg gacgacgacc
     3721 gagacggcgg ccgcctcgac ctcctcggcg gagcgggcgg cataggcttc gacaagctcg
     3781 gtgacggaat gcgtccgccc ggccttggcc ggaaacggat ccacgccggc cgcgatcagg
     3841 cggcggtatt tatccgcccg gacgagctcc tgctcgcttc gccggagctc atcggccgga
     3901 cggggtccgc cgtccgcctc cgcggggggt tccggcgggc cggggagggg tttggggttt
     3961 tggggatcgg tcatgttcat tcttctcgga tgtgtgattt gctccggctt cgccggaaag
     4021 cgcccgccgg acggcgtcga gaacgccgtt gacgaaaacc gccgattccc cgccgctgaa
     4081 gcggcgggcg atctcgacgg cctcgttgat gatgatggcc ggaacgagcg ttttttcctc
     4141 gagcatttcg tagacggcca ggcggaggat gttccggtcg accagcccca tccgggccac
     4201 ccgccaattg cgggccgcgc cctggatgcg gtcgtcgagc tccgatcgcc ggccgaaaac
     4261 tccccggacg agccaggcgg cgtactcccc cgccccggcg ccggccggtt tctccgcttt
     4321 cctgcgccgc aggacctcct ccggacccga ctcgttgaat tcgagctcgt aaaggatctg
     4381 gagggccgtt tcccgggcgc cgcgccgtct gcccatgcct tacagcttgg cggccttgat
     4441 gagggtgagc agctcgacga gcgagccgac ggcgtcccag cccttgttgc cggccttcgt
     4501 tccggagcgt tcgatgcctt gctcgatcga atcgacggtc aggacgccca tggtcaccgg
     4561 aacgcccgtt tccagggaga tctgggacaa gctcttggtc acctcggccg ccaggaaatc
     4621 gaagtgcggc gtttcgccgc ggatcagcgc gcccaggcag acaaggccgt cgacccggcc
     4681 cgaggcggcg agcttcttga cgacgagggg ggcttcgaaa gaccccggga ccttgcagac
     4741 cgtgatgtcg gcctcctcgg ccccgagctt ccggagggcg tccaaggcgc cgtccaggag
     4801 cttgtcggtc aggaaggaat tgaagcggct gacgacgatt ccgatcttca gccccttggc
     4861 ctgcagcttt ccttcatgta ttttcatcgt ttctcccttt cgcctccggc gccgcctcgt
     4921 cgccgaatcc gggtcgacgg gcggtcagca agggggcttg tcaggtgcgg tttattttaa
     4981 cagaagcagg ttcgttttgc acttcccccg gccgggcggg atcatcggaa ctattcaaga
     5041 ccctcagata gcccgccgtg cacttcctcc ggccggacgg gatcatctcc ggtccaagga
     5101 cgtgtcctcg ccgccgttcg cgaggagcgg cctcggccgt tcccggaaat gcctttcccg
     5161 ggggaacgac gcttattcga gaagggctcg gct
//
LOCUS       DAOOXH010000132         5091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_19063_length_5091_cov_7.186656, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000132 DAOOXH010000000
VERSION     DAOOXH010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19063_length_5091_cov_7.186656"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="PKV64_10900"
     CDS             <1..698
                     /locus_tag="PKV64_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17623.1"
                     /translation="TPIFLLNGVLSVVLIPVIFILFARGGGGGSSGDAFQIILLLASS
                     NPAAVILGSALFMTICGCLNGTASSAFSREGAHFWMSKVIPVSPADQVKAKFLHSFAV
                     AGLGILVAAGAAVAVIGAKPGCVLLAMPLALAAAAALTSVGMIIDLSRPLLKWTNPQK
                     AIKQNFNVLIAFLADAGLLIILTLAVTALYGEGLGGAGIIGGLAVFLAGLSFLSFSFL
                     LRIAGRRYAEIEA"
     gene            complement(739..4383)
                     /locus_tag="PKV64_10905"
     CDS             complement(739..4383)
                     /locus_tag="PKV64_10905"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HPN17624.1"
                     /translation="MNKRLRLGAAAAASFLLVFLLQSRPAGGQACVENSGTVTETLQT
                     GTNLDLPQCSAKLWYADDANPRDIMTLNKLGANFAVSQPASVPGWISVIAAGDFNLDG
                     WIDYIGSSSEYSNALLFVRNDGVNGHIGTFVADLWIDGSTGNSSGIPTRGVGGAAIES
                     GYHGSMTAGDYDGDGDLDFFYVTSVFNSPYTLVHVWLFENRLIQNGVNTGVLEFVRTD
                     LTSAWRPLLKTLGWSSTGIVSLDLDGDGDVDMAYGNTEGKVFKITNTGSGKINASTFA
                     VDYVMVATGWTGLGVSTISIAEFDESPGLDMIVGSCDYPELKYYRGDGLGGFEWLADF
                     TDPTGDLYNNMYDGAATISLCDDFDGDGDIDLIIGTDNWCWNGVRGYPGQSGIATGYG
                     GKCYYFKNGGNADFTVSLVFDGQVKSPKVYDFDCGLILDYNNDGQMDFLMADGNHTDY
                     YYVFLNSIADVYNLKGVGLSTNLTPSLPGTQYAITRARFTIIDQGVIGSSATGLSVTY
                     YLSNDDGYSWEYYGRYSGSAIANVTNQAWHDFHSFGSKLRWKAVLTAPDDAIPGFGES
                     SYETPSVDRLAIEYVYVERREYSRSSAAAAAIISGTRRKLIISSSFMFPGYEGQLRAY
                     DVTDIGLASTGGSTLQTISTADPLSPTGRNVAAGGSILWDAGQLLAAREASDRTIFTA
                     YKPSGTLTRIDFTEGNVNVLKSLLSDPNGDNAGLIRFVRGEGRTWKLGDILHSSPVLM
                     GPPSGDAVALGSSYSEFAEDNESRTPVVFIGANDGMLHCFDLASGTELWGFIPFNLIP
                     KLKNMSVRDANGARLYKHDYFVDGTPTVGDAYINGQWRTVLMCGQGAGKGSTGAGGLN
                     YYFALDVTDPSNPVPLWEAKDSRTMGETWSVPAFGQVVSRTTPHWVAFVGSGYDNDTA
                     RIIGNRFYVIRLDTGAILKTLTVATDVDTSLNNKCPRPYPNIQVTVPGSPTAVDINRD
                     GRTDYVYFGDLDGRLYRVPFTSTNANNWALQTIYTDRMSYPIITKPAVFPDPASGGLP
                     LKILFGTGGDDRAPGDRTYAFIGLTDTGSKASVEWYIGNKDELGLAASLQTGEMATGE
                     KIWSDPVISDRIVYFSSLMGSIENVNPCLNLAETGRLYARYVQTAAGCVIGESGLKSA
                     GGSSIESLQLASKARRAVTVGELQAGGGSSSKKEVYIQEYDSTIERLEQPVGSNLRVV
                     SWREIYKIIR"
     gene            complement(4397..5002)
                     /locus_tag="PKV64_10910"
     CDS             complement(4397..5002)
                     /locus_tag="PKV64_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17625.1"
                     /translation="MNRRTARHRNRERGAGLFIVILIVAGLAALGMTLLTLTSMGPKM
                     SGGLRSQEEAFNAAEAGFNAAQALIKQYFGDGSWLNFEGHTLTQPSNIDRPLIGTNIN
                     PNYFRRVPDEEILLALDPGKDGVPDYGPLLFFNQTFAATETGATDPRLSYTAFLINDE
                     AAGGAADPNDVLLVVIGVVRSGSRILATTRLEIVLAYVAGV"
BASE COUNT          875 a         1571 c         1670 g          975 t
ORIGIN      
        1 ggacgccgat tttccttctc aacggcgttc tctcggtcgt cctcatcccg gtcatcttca
       61 tcctcttcgc ccgggggggc ggcggcggct caagcggcga cgcgttccag atcatccttc
      121 tcctggcctc gtcgaatccg gcggcggtga tcctcggctc ggccttgttc atgacgattt
      181 gcggctgctt gaacggcacg gcgtcctccg ccttctcccg ggagggcgcc catttctgga
      241 tgtcgaaggt catcccggtt tcgccggccg atcaagtcaa ggctaaattc ctccactcct
      301 tcgccgtcgc cggcctgggc atcctggttg cggccggggc cgccgtcgcc gtcatcgggg
      361 caaagcccgg ctgcgtcctc ctggcgatgc ctctcgccct ggcggccgcg gccgccctga
      421 cctcggtcgg tatgatcatc gatctttccc ggccgctttt gaaatggacg aatccgcaaa
      481 aagccatcaa gcagaacttc aacgtcctga tcgccttttt ggccgacgcg gggctgttga
      541 tcatcctgac cctggccgtg acggccctgt acggggaagg actcggcggc gcgggaatca
      601 tcggagggct ggccgtcttc ctggccggct tgtccttctt gagcttctct ttcctgctga
      661 gaatcgccgg ccggcgttac gcggagatcg aggcttgact tctgtcccgg ccgggccggg
      721 agcggcggaa gcggcgcctt accggatgat cttgtagatt tcgcgccaag agacaacccg
      781 gaggttcgac ccgaccggct gctccagccg ctcgatcgtg gaatcgtact cctggatgta
      841 gacttccttc ttggacgacg acccgccccc ggcctgaagc tccccgaccg tgacggcccg
      901 ccgggccttg ctggccagct ggaggctttc gatcgacgac ccgccggccg atttgagtcc
      961 cgattcgccg atgacgcacc cggccgccgt ctggacataa cgggcataca aacggccggt
     1021 ctcggcaagg ttgaggcatg gattgacgtt ttcgatgctg cccatcaggg agctgaaata
     1081 gacgatcctg tccgagatga cggggtcgga ccaaatcttc tccccggtcg ccatctcccc
     1141 cgtctgaagc gaggcggcca gccccagctc atccttgttc ccgatatacc actccacgct
     1201 cgccttggag cccgtgtcgg tgaggccgat gaaggcatag gtccggtcgc ccggcgcccg
     1261 gtcgtcgcct cccgtcccga agaggatctt caaaggaagg ccgcccgaag ccgggtccgg
     1321 aaaaacggcc ggcttggtga tgatcgggta ggacatccgg tcggtgtaga tggtctgcag
     1381 cgcccagttg ttggcgttcg tgctggtgaa gggcacacga tagagacgcc cgtccaggtc
     1441 tccgaaatac acatagtccg tccgcccgtc ccggttgata tccacggccg tgggcgaacc
     1501 gggaacggtg acctggatgt tcgggtaagg ccgcgggcat ttgttgttca ggctcgtgtc
     1561 gacgtcggtc gcgacggtca gggtcttgag gatcgctccc gtgtcgagcc ggatgacgta
     1621 gaaccggttg ccgatgatcc gggccgtatc gttgtcgtac ccggagccga cgaacgccac
     1681 ccagtgcggc gtcgttctcg acacgacctg gccgaaggcc gggaccgacc aggtttcgcc
     1741 catggtccga gaatccttcg cctcccaaag aggaacggga ttgctcgggt cggtgacgtc
     1801 cagggcgaaa taataattca ggccgccggc cccggtgctg ccctttcccg ccccctggcc
     1861 gcacatcagg accgtccgcc actgcccgtt gatataggca tcgcccacgg tcggcgttcc
     1921 gtcgacgaaa taatcgtgct tgtacagccg ggctccgttg gcgtccctca ccgacatgtt
     1981 cttcagcttg gggatgagat tgaaggggat gaatccccag agctcagtgc ccgaggccag
     2041 atcgaagcag tggagcatcc cgtcgttggc tccgatgaag acgaccggcg tccgggattc
     2101 attgtcctcg gcgaactcgc tgtaggatga tcccagggcc acggcgtccc ccgacggcgg
     2161 tcccatcaag accgggctcg aatggaggat atcgcccagc ttccaggtcc ggccctcgcc
     2221 gcggacgaag cggatcaagc cggcgttgtc gccgttgggg tcggagagaa gcgatttgag
     2281 gacgttgacg ttgccctcgg tgaaatcgat ccgggtgagc gtgcccgagg gtttgtaggc
     2341 cgtgaagatc gtccggtcgg acgcctcgcg cgccgccaga agctggccgg cgtcccacag
     2401 gatggatccg ccggccgcga cattccgccc cgtcgggctg agcggatcgg ccgtggaaat
     2461 cgtctggagg gttgatccgc ccgtgctggc caagccgatg tcggtcacat cgtaagcccg
     2521 gagctgtccc tcgtatccgg ggaacatgaa ggaggaggag atgatgagct tccggcgggt
     2581 gccggaaatg atggccgcgg ccgcggccga cgaccgggaa tactcccgcc gttcgacata
     2641 gacgtattcg atcgccaggc ggtcgacgga cggcgtttcg taagacgact cgccgaagcc
     2701 ggggatcgcg tcgtccgggg ccgtcaggac ggccttccag cggagcttgg agccgaagga
     2761 atggaagtcg tgccaggctt ggttggtgac attggcgatc gcggatcccg agtaccggcc
     2821 gtaatattcc caggaatatc cgtcgtcgtt ggacaggtaa taggtgacgg aaagaccggt
     2881 ggccgaggag ccgatgacgc cctggtcgat gatcgtgaag cgggcccggg tgatggcgta
     2941 ctgagttccc ggcagggacg gcgtcaggtt cgtcgagagg ccgacgccct tcagattgta
     3001 aacgtcggcg atggagttga gaaaaacgta atagtagtcg gtgtggttgc cgtcggccat
     3061 gaggaaatcc atctggccgt cgttgttgta atcgaggatc aggccgcagt cgaagtcgta
     3121 gaccttgggg cttttcacct gcccgtcaaa gaccaggctg acggtgaaat cggcgttccc
     3181 tccgttcttg aaataatagc acttgcctcc gtagccggtg gcgatgcccg attggccggg
     3241 gtagccgcgc acgccgttcc agcaccagtt gtccgtcccg atgatcaggt cgatgtcccc
     3301 gtcgccgtcg aaatcgtcgc ataagctgat cgtggccgcg ccgtcgtaca tgttgttgta
     3361 gagatccccg gtggggtcgg tgaagtcggc cagccattcg aatcccccca ggccgtcccc
     3421 gcggtagtac ttcagctcgg ggtaatcgca gctgccgacg atcatgtcca ggcccggcga
     3481 ctcgtcgaat tcggcgattg agatcgtgct gacgcccagc ccggtccagc ccgtcgcgac
     3541 catgacgtaa tcgacggcga acgtcgaggc gttgatcttg ccggagccgg tgttcgtgat
     3601 cttgaagacc ttgccctcgg tgttcccgta ggccatgtcg acgtcgccgt cgccgtcgag
     3661 gtccagggaa acgatccccg tcgacgacca gcccagggtt ttgagaagcg gccgccaggc
     3721 cgacgtcaag tcggtccgga cgaactccag aacgccggtg ttgacgccgt tctgaatgag
     3781 acggttttca aaaagccaga catggacgag ggtgtagggc gaattaaaga cggacgtgac
     3841 gtagaaaaag tcgaggtctc cgtcgccgtc gtaatcgccg gccgtcatcg agccgtggta
     3901 tcccgactcg atggccgccc cgccgacgcc ccgcgtcggg atcccgctgg aattcccggt
     3961 cgagccgtcg atccagaggt cagcgacgaa ggtcccgata tggccgttga ctccgtcgtt
     4021 gcggacgaag agcagggcgt tggagtactc gctgctcgac ccgatgtaat cgatccagcc
     4081 gtccaggttg aaatctcccg cggcgatgac gctgatccaa ccggggacgc tcgccggctg
     4141 gctgacggcg aagttcgccc cgagcttgtt gagggtcatg atgtcccggg gatttgcgtc
     4201 atcggcgtac caaagcttgg ccgagcattg cggcaggtcg aggttcgttc cggtctgaag
     4261 agtctccgtg accgtcccgg aattttcgac gcaggcctga ccgccggccg ggcggctctg
     4321 aagcaggaaa acgaggagga aggaggccgc ggccgcggct ccgaggcgaa gtcgtttgtt
     4381 catgtcgatt ctccctttaa acgccggcga cataggccaa gacgatctcc agccgggtcg
     4441 tcgccaggat ccgcgatccg gaacggacga cgccgatgac gaccaggagg acgtcgttcg
     4501 gatcggcggc tcccccggcg gcttcgtcgt tgatcaggaa cgccgtgtag gacaggcgcg
     4561 ggtcggtcgc cccggtctcg gtcgcggcga acgtctggtt gaagaacagg agggggccgt
     4621 aatcgggcac cccgtccttt cccgggtcca gggccagaag gatctcctcg tcgggcacgc
     4681 ggcggaaata attgggattg atgttggttc cgatgagagg gcggtcgatg ttcgacggct
     4741 gggtcagggt gtggccctcg aagttcagcc aggatccgtc gccgaaatac tgcttgatca
     4801 gggcctgggc ggcgttgaat ccggcctcgg ccgcgttgaa agcctcttcc tgggaccgca
     4861 gcccccccga catcttcggc cccatcgacg tcagggtcag cagggtcatg cccagggcgg
     4921 ccaagccggc gacgatgagg atgacgatga agagccccgc cccccgctcc cggttccggt
     4981 gccgcgccgt ccgcctgttc atcgcaagcc cgtgttgaag agattcacgg aaaggttccg
     5041 gatgttcgcg gtcgattcca agaggaaacg cttccgccaa tcgggggccg a
//
LOCUS       DAOOXH010000133         4968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_19691_length_4968_cov_6.362711, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000133 DAOOXH010000000
VERSION     DAOOXH010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19691_length_4968_cov_6.362711"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="PKV64_10915"
     CDS             <1..1100
                     /locus_tag="PKV64_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HPN17626.1"
                     /translation="MPLAVQWWSVWYPGAEPGGGSYIAQRMLASKSEKDSLGAVLFFN
                     LAHYVLRPWPWILVALCSLVVFPNLADIQAAFPGLDPTLLGHDIAYPAMFKFMPIGFF
                     GLIIGGLIAANSSTVLTHLNWGASYLVHDFYRRFLRRDATEKHYVLVGRLATIFLFFA
                     SSALVFVLETAKDSFDIILQVGAGTGLLYLARWFWWRVNAWCEVVAMVSSFLVSIGLL
                     VLRRAGTVVPTHYALMITVAVTTVCWVATAFLGPRTDKKTLLDFYKKVRPFGPGWKAV
                     RAEAGLAAGGGRGPHESIPLALVGWSTGCAVIWSGLFLVGNILYGRWNYAAILGAVFI
                     ACGLVLIRVVNRLWTNKAETAAAAAGEGKES"
     gene            1097..2107
                     /locus_tag="PKV64_10920"
     CDS             1097..2107
                     /locus_tag="PKV64_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HPN17627.1"
                     /translation="MNIGRFYRKAVAAGIAADLRGKKEIDRLLAEAAEEYKGAAAEDK
                     EFFDRDRLFNPYADTRLLTGDPETPVRRILAGIDIDGGELHLARSLNREPAGPKIDLV
                     LSHHPLGPAQYRMYDVMRLQAGLLALHGVTVSVAEQLMDKRIEEVERRLLPANATRTM
                     DLARALGLPLICLHTVADNCVTNYLTKLFAAKKPSRLKDLVRLLRAIPEYERSFRLQA
                     PPKIINGKDSHSCGKIFVDMTGGTEGAKDIFDKLVSGGVSTIVGMHMSEDHLANARKA
                     GINVVIAGHIASDVLGLNLLLDDVEKDGRLDYVCVSGFERIRRMKPESKQAGRRRSPP
                     PR"
     gene            complement(2321..4948)
                     /locus_tag="PKV64_10925"
     CDS             complement(2321..4948)
                     /locus_tag="PKV64_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPN17628.1"
                     /translation="MLKKDSLKAGAAALFALLLAPLFLPAQTSPEAFLGFKIGADRKL
                     ADYEQIQAYFKKLASESPKIRIETIGTSTLKRPMIMAVVTAEENMARLDEYKAISAKL
                     KDPRTLPAAEAEKLARDGKVIVLVTCSLHATEIAASQMSMELAYKLVTGDTPFDAAAV
                     LRDVIFLLVPTSNPDGNQMEVDWYKKNLGTKFEGGDMPWLYHHYAGHDNNRDWFMSNL
                     SETRAVNQVLYHDWFPQIHLDQHQMGSDGARLFIPPFMNPPVPNVQPLVWRGVNLLGA
                     NLGYDLQKNGFKGVVHGRSFTGWYIGACDDTSWLHNILGLLSEMASVGLATPLFIEPT
                     EIPTSYTQKRIEFPDPWPGGWWRLRDIVDYELTLSLSLLRTASLYKEEFLLNTYRMNK
                     RSVETVETGQPYAFVVPAVQPDPPTMLKMLDVLQMGGVEIHRAKKEFRAGNRVYPAGS
                     FVILMAQPNKPFAWALLERQKYPDIREYAGGPPAVPYDNAGWTLPLQMGVACEEIGKP
                     FAAELEKIDRTPSPDIVVPGEATPWLVFDCRTNASYAAVFALLKDKAEVHRSKGKIIG
                     SGFEAAPGSFIVKNTPAVQKALPALLEKLRLSVHPLAEADALPKSVLKNPRIGLYQSW
                     WSSQDEGWTRYMFDDLGVPYTTLHNADFKAPKAGKAKEPAKVDLKARFDVIVFAGEPY
                     DLIKTGKVDPASPWARWFSPLPPEYEGGLEKEGIDNLKAFVEDGGILVTLNEACKLAL
                     KDFEPPARDILDKADRTKFFCPMSILKISVDNATPLGYGMPETAAAVFSGSPALETWI
                     PQTADWDRKVVASYPEEDLLLSGWIKGEETIARKAAVVDAPHKKGRVVLIGIACQSRA
                     QSHGTYKFLLNALLYPEMP"
BASE COUNT          832 a         1531 c         1644 g          961 t
ORIGIN      
        1 tcatgcccct cgccgtccag tggtggtcgg tctggtatcc gggggccgag cccggcggcg
       61 gcagttatat cgctcagcgc atgctggcct ccaagtcgga aaaggactcg ctgggcgcgg
      121 tcctcttttt caacctcgcc cattacgtcc tgcggccctg gccctggatc ctggtcgccc
      181 tttgttcgct ggtcgtcttc cccaacctgg ccgacatcca ggcggccttt ccggggctcg
      241 atccgacgct tctcgggcat gacatcgcct atccggccat gttcaagttc atgccgatcg
      301 gcttcttcgg cctcatcatc ggcggcttga tcgcggccaa ctcctccacc gtcctgaccc
      361 atctcaactg gggggcctcc tatctcgttc atgactttta tcggcgcttt ctccgccggg
      421 acgccaccga aaagcattac gtcctggtcg gccggttggc cacgatcttc ctgtttttcg
      481 cctccagcgc cctggtcttc gtcttggaaa cggccaagga cagcttcgac atcatcctcc
      541 aagtcggggc cgggacgggc ttgctgtact tggcccgctg gttctggtgg cgggtcaacg
      601 cgtggtgcga ggtcgtggcc atggtcagct cgttcctcgt ctccatcggc ctgctcgtcc
      661 tgcgccgggc cggaaccgtc gtcccgaccc attacgccct gatgatcacg gtcgccgtga
      721 cgacggtctg ctgggtggcc acggctttcc tcggcccgcg gaccgacaag aaaacgctcc
      781 tcgatttcta caagaaagtc cgtccgttcg ggccggggtg gaaggccgtc cgggccgagg
      841 ccggattggc cgccggcggc ggccgggggc cgcatgaaag catccctctg gccctcgtcg
      901 ggtggagcac gggatgcgcg gtgatttggt cgggcttgtt tttagtgggc aacattctgt
      961 acggccggtg gaattacgcc gcgatcctcg gcgcggtctt catcgcctgc ggcctcgtcc
     1021 tgatccgggt ggtcaaccgc ctgtggacga acaaggccga aacggcggcc gcggccgccg
     1081 gggagggaaa ggaatcatga acatcgggcg gttttatcgg aaggccgtgg ccgcggggat
     1141 cgcggccgac ctgcggggga agaaggaaat cgaccgcctc ttggccgagg cggccgagga
     1201 gtacaagggc gccgccgcgg aggacaagga attcttcgat cgggaccgcc tgttcaatcc
     1261 ctatgccgac acgcgcctgc tgaccggcga tccggagacg ccggtccgcc ggatcctcgc
     1321 cgggatcgat atcgacggcg gcgagctcca tctggcccgt tccctcaacc gggagccggc
     1381 cggtccgaag atcgacctgg ttttaagcca tcaccccctg ggtccggccc agtaccggat
     1441 gtacgacgtg atgcgcctgc aagccgggct gctggcgctc cacggggtga cggtctccgt
     1501 cgccgagcag ttgatggaca agcggatcga ggaagtggag cggcggcttc tgccggccaa
     1561 cgccactcga acgatggacc tggcccgggc cctcggcctg cccctcatat gcctccacac
     1621 ggtcgccgac aattgcgtga cgaactacct gacgaaactg ttcgcggcga aaaaaccgtc
     1681 ccggctcaag gatctcgttc gtctgctccg ggcgatcccc gaatacgaga ggtccttccg
     1741 cctccaggct ccgccgaaga tcatcaacgg caaggactcc cattcctgcg ggaagatctt
     1801 cgtcgacatg accggcggga ccgagggagc caaggatatc ttcgacaagc tggtttccgg
     1861 cggggtgagc acgatcgtcg ggatgcacat gtccgaggat catctggcga acgcccggaa
     1921 ggccgggatc aacgtggtca tcgccgggca tatcgccagc gacgtcctcg gcctgaacct
     1981 gctcctggac gacgtcgaaa aggacgggcg cctggactat gtctgcgtca gcggcttcga
     2041 gcgcatccgg cggatgaagc cggaatcgaa gcaagccggg cgacgccgtt ctccgcctcc
     2101 ccggtgattt ccggtcccgg ccgaagcgag gttccgatca tcgggatctc cgagacggct
     2161 ccccgctcgg ccgggttctt caggcctctt ccctcgaggg gaaaccagcg agaagacagc
     2221 ggcacccgtc aggagccgag tcgaccctcg gtctcctctc gatcgccgca tcccgtttta
     2281 atcgtgaggc cgaaattctc gttcgtttgc ggcgtctccc tcagggcatc tccggataaa
     2341 gaagcgcgtt gagcagaaat ttgtaggtcc cgtgcgattg ggcccgggac tggcaggcga
     2401 tgccgatcag gacgacgcgc ccctttttat gcggagcgtc gaccacggcc gccttccggg
     2461 cgatggtctc ctcgcccttg atccacccgc tgagcagaag atcctcttcg ggatagctgg
     2521 ccacgacctt ccggtcccaa tcggccgttt ggggaatcca agtctccaga gccgggctcc
     2581 cgctgaagac cgcggccgcc gtctccggca ttccgtaccc gagaggcgtc gcgttgtcga
     2641 cggaaatctt cagaatcgac atcgggcaga aaaatttcgt ccggtcggcc ttgtctaaga
     2701 tgtcgcgggc gggcggctcg aaatccttga gggccagctt gcacgcttcg ttcagggtga
     2761 cgaggatccc gccgtcctcg acaaaagctt tgaggttgtc gatcccctcc ttttccaggc
     2821 cgccctcgta ctcgggcggc agcggagaga accaccgggc ccaaggcgag gcgggatcga
     2881 ccttgcccgt cttgatcagg tcgtagggtt cgccggcgaa gacgatgacg tcgaaccggg
     2941 ccttgaggtc gaccttggcc ggctccttcg ccttgcccgc cttcggcgcc ttgaaatcag
     3001 cgttgtggag ggtggtgtag gggactccca ggtcgtcgaa catatagcgc gtccagccct
     3061 cgtcctggct ggaccaccag gactggtaga ggccgatgcg cggattcttg aggaccgact
     3121 tcggaagagc gtccgcctcg gcgagcggat ggacggaaag acgcagcttt tccaaaagcg
     3181 ccgggagggc cttctggacg gccggcgtgt tcttgacgat gaagctgccc ggcgcggcct
     3241 cgaaaccgga cccgatgatc ttgcccttgg accggtggac ttcggccttg tccttgagca
     3301 gggcgaagac ggccgcgtag gaggcgttcg tccggcaatc gaagaccagc cagggcgtcg
     3361 cctcgccggg gacgacgata tccggggacg gcgtccggtc gatcttttcg agctcggccg
     3421 caaacggctt gccgatttcc tcgcaggcga cgcccatctg cagcggcaga gtccagccgg
     3481 cgttgtcgta ggggacggcg ggcggcccgc ccgcgtattc acggatatcg ggatacttct
     3541 gccgctcgag cagggcccag gcgaacggct tgttaggctg ggccatgagg atgacgaagg
     3601 atccggccgg gtagacgcgg ttgccggccc ggaattcctt cttcgcccgg tggatttcca
     3661 cgccccccat ttgaaggaca tcgagcatct tgagcatcgt cggggggtcg ggctggacgg
     3721 ccggaacaac gaaggcgtac ggctgtccgg tctcgacggt ctcgacggag cgcttgttca
     3781 tccggtaggt gttgagcagg aattcctctt tatacaagga ggccgtgcga aggaggctca
     3841 gggaaagcgt cagctcgtaa tcgacgatgt cccgcaggcg ccaccagccg ccgggccagg
     3901 gatcgggaaa ctcgatccgc ttttgcgtgt aggatgtcgg gatttcggtg ggctcgatga
     3961 agaggggcgt ggccagcccg accgaggcca tctcgctcag caggccgaga atgttgtgga
     4021 gccaggaggt gtcgtcgcag gctccgatgt accagccggt gaagctccgg ccgtgaacga
     4081 cgcccttgaa gccgtttttc tggaggtcgt agcccaggtt ggcgccaagc aggttgacgc
     4141 cccgccagac gagcggctgg acattcggga cgggaggatt catgaacggg gggatgaaga
     4201 gacgggctcc gtcggacccc atctggtgct ggtcgaggtg gatctggggg aaccagtcgt
     4261 gataaagaac ctggttgacg gcccgcgttt cactgaggtt ggacatgaac cagtcgcggt
     4321 tgttgtcgtg gccggcgtaa tgatggtaga gccagggcat gtccccgccc tcgaacttgg
     4381 tcccgaggtt tttcttgtac cagtcgactt ccatctggtt gccgtcggga ttggacgtcg
     4441 ggacgaggag gaaaatgacg tcccggagga cggccgcggc gtcgaagggc gtatccccgg
     4501 tgactaattt ataggccagc tccatcgaca tctgggaggc ggcgatctcc gtggcgtgga
     4561 ggctgcacgt gacgaggacg atgaccttgc cgtcccgggc cagcttctcc gcttcggccg
     4621 ccggaagggt ccgtggatcc ttaagcttgg cggaaatcgc tttgtattca tccagccggg
     4681 ccatgttttc ctcggccgtg acgacggcca tgatcatcgg ccgcttcagg gtggaggtcc
     4741 cgatggtctc gatcctgatc ttcggcgatt ccgaggccag cttcttgaaa taggcctgga
     4801 tttgttcgta gtcggccagc ttgcggtcgg cgccgatctt gaagcccagg aatgcttcgg
     4861 gcgatgtttg agcggggagg aacaggggcg cgaggagaag ggcgaagagg gcggccgcgc
     4921 cggctttcag ggagtctttc ttcaacatga aaacctccat gcgcgaat
//
LOCUS       DAOOXH010000134         4821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_20509_length_4821_cov_6.189257, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000134 DAOOXH010000000
VERSION     DAOOXH010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20509_length_4821_cov_6.189257"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PKV64_10930"
     CDS             <1..691
                     /locus_tag="PKV64_10930"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPN17629.1"
                     /translation="RLVGRGPAGRKAVLDAYLGKAPAEFTWKEKTYTPQTFLSDVLGL
                     NLDDYIELTSYDSHPFFKKCRLEIPDNWNYNSDYYNLPLDDLEAVVEKAISDGYTVAW
                     DGDVSERDFSTRETGWALVPDTDWEDKTMAEREKKPAAPVKEKEITPAMRTATFRDFT
                     TTDDHLMHIVGLAQDQTGARFYLIKNSGGTDRKYDGYMYMSRAYFRLKTTALMVNKNS
                     LAPDLRAKLGI"
     gene            complement(726..1628)
                     /locus_tag="PKV64_10935"
     CDS             complement(726..1628)
                     /locus_tag="PKV64_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPN17630.1"
                     /translation="MTGSRVHRRPDSHRAFAAGVTLNTLFVLAEGGVGAAVGSLALIA
                     DAGHNFSDVISLLLAWGAGALAQRAATERRTYGFRKATVVASFISAVLLLAALGAIAR
                     EAVGRLLHPAPVGGPVIILVAAIGAVINAATAALFFSGKKNDLNIQAAFLHMAADAGV
                     SLGVVAGGILIALKGWPWIDPAISLAIVVIVFWGTWKLFRRSLDLTLDAVPENIDIAG
                     IKGYLAGLENVVQIHDLHVWALSTTEVALTVHVVAAADSFDTNTLCGIQRHLHDRFGI
                     AHATIQVETPTAGDGCLLDRPDCL"
     gene            complement(1640..2836)
                     /locus_tag="PKV64_10940"
     CDS             complement(1640..2836)
                     /locus_tag="PKV64_10940"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HPN17631.1"
                     /translation="METIRELYRIGPGPSSSHTMGPRAAADMFLARVPAAPAYRVTLY
                     GSLAATGKGHLTEETLREEFGGRKIEIVWRPAERLPLHPNALRFEALDDEGRVQEFWT
                     AYSIGGGEIVDGTPATVRRSIYPLHSMDEIVRSCVQNGRTLWETVEEVEGPGIWDFLA
                     GVWAAMSEAVRRGLKTEGTLPGDLRLSRKARSYFMKAKMFAGHSKRMGLLFAYALAVA
                     EENAAGGVIVTAPTCGSAGVVPAVLTHLRKEHRFSDREILRALAAAGLIGNLAKTNAS
                     ISGAEVGCQGEIGVACAMASGAAAQLLGGTVPQIENAAEMGIEHHLGLTCDPLHGLVQ
                     IPCIERNVFAASRAVSHAAYALLSDGRHRISYDEVLEAMRQTGKDLSPRYKETSTGGL
                     AVVDKE"
     gene            2968..4614
                     /locus_tag="PKV64_10945"
     CDS             2968..4614
                     /locus_tag="PKV64_10945"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPN17632.1"
                     /translation="MRKNSDSKLIILAAAGFLAFAAFAAAQTATAPDPKAVALRGAYG
                     PFRANNDLLSYDLDIRVDPEAKAWSGKNTIRFRMLKEDARIQLDLKAHLTVEKITLDG
                     APLPFTREYDAVFVDFPEPLKKDSVTAIDFYFSGRASGEKDWGGITFKTDPAGRPWIT
                     TACQGSGAMTWWPNKEQQRDEVERMGLRVAVPNGLVDVSNGRFAGRTDLGDGYTRWDW
                     EVHYPINNYNVALNIAAYVHFEDRMDGLTLDYYCLPENLEKAKVQFAQARTTIECFEK
                     YFGPYPFPEDGYKLIEVPYAGMEHQSAVAYGNRFLNGYLERDFTGVGVSLKFDYIIIH
                     ESAHEWFGNSITAADVCDEWIHEAWATYMEGLYVEHLFGYEDAVKYLNGYRPKVKNER
                     PIIGPPDVHYNAPQDMYFKGALFLNTLRHIVDDEAKWRTLLRGFYDRFKYQNIGSEDA
                     FAFLNVETGLNLGPVFDQYLRRTTIPALELKLDEAAGSVSFRWKSEVPGFDMPVKAGR
                     RGQWLTLRPSAEWQTMKTDLKKDEFEVADDLFYIDVVRID"
BASE COUNT          922 a         1597 c         1517 g          785 t
ORIGIN      
        1 ccgccttgta ggccgcgggc cagcggggcg taaggcggtc ctcgacgctt atctgggcaa
       61 agcgccggcc gagttcacct ggaaagagaa aacctatacg ccccagacct tcctctccga
      121 cgttctgggt ttgaacttgg acgattatat tgagttgacc tcctatgaca gccacccttt
      181 cttcaaaaaa tgccgcctgg aaatccccga caactggaac tacaattccg actattacaa
      241 ccttcctctc gatgacctgg aagccgtcgt cgagaaggcg atttccgacg gctacacggt
      301 cgcctgggac ggcgacgtga gcgagcggga cttctccacg cgcgagaccg gctgggccct
      361 ggtgcctgat acggactggg aggacaagac catggccgag cgcgagaaaa agccggcggc
      421 gccggtgaag gaaaaggaga tcaccccggc gatgcgcacg gccaccttcc gcgacttcac
      481 caccaccgac gaccatctga tgcacatcgt cggcctggcc caggatcaga ccggcgccag
      541 gttctacttg atcaagaact cgggcggcac cgaccgcaag tatgacggct acatgtacat
      601 gtcgcgcgcc tacttccgcc tcaagaccac ggccctgatg gtcaacaaga attcgctcgc
      661 ccccgatctg cgggccaagc tgggaattta atcccggcgc cggcggccgc tccgccgccc
      721 tggtcttaca ggcaatccgg ccggtcgagg agacagccgt cgccggccgt cggagtttcc
      781 acctggatcg tcgcgtgggc gatgccgaag cgatcgtgca gatgccgctg gatcccgcac
      841 agggtgttcg tgtcgaagga atcagcggcc gcgaccacgt gaacggtgag ggcgacctcc
      901 gtcgtgctca gggcccagac gtggaggtcg tggatctgga cgacgttttc caggccggcc
      961 aggtaaccct tgatcccggc gatatcgatg ttttcgggaa cggcgtccag ggttaagtcg
     1021 agcgagcggc ggaacagctt ccaggtcccc caaaagacga tgacgacgat cgccaggctg
     1081 atcgccgggt cgatccacgg ccagcctttc agggcgatga ggattccgcc ggcgacgacc
     1141 cccagggaaa cgccggcatc ggcggccata tggagaaacg ccgcctggat attcaagtcg
     1201 ttctttttcc cggagaaaaa gagagcggcc gtggccgcgt tgatgaccgc gccgatcgcc
     1261 gcgacgagga tgatcaccgg ccccccgacc ggcgccgggt gaagaagacg gccgaccgcc
     1321 tcccgggcga tcgcccccag ggccgcgagc aggagaacgg cgctgatgaa ggaggcgacg
     1381 accgtggcct tgcggaaacc gtacgtccgc cgctccgtag ccgctctctg ggccagggcg
     1441 ccggcccccc aggctaaaag aaggctgatc acgtcgctga agttgtgtcc ggcgtcggcg
     1501 atcagggcca gggatccgac cgccgctccc acgccgccct cggcaagaac gaacagggta
     1561 ttgagcgtca cgccggcggc gaaggcccgg tgggagtccg ggcggcgatg gacgcggctt
     1621 ccggtcatgc ccggcctcgc tattccttat cgacgacggc caggccgccc gtcgaggttt
     1681 ccttgtagcg cggggacagg tccttccccg tctggcgcat cgcttccagg acctcgtcgt
     1741 agctgatccg gtgccgtccg tccgaaagca aggcgtacgc cgcgtggctg accgcccggg
     1801 aagcggcgaa gacgttccgc tcgatgcacg ggatctggac aagaccgtgg aggggatcgc
     1861 aggtcaggcc gagatggtgc tcgatgccca tttcggcggc gttctcgatc tgggggacgg
     1921 taccgcctag aagctgggcc gccgcccccg aggccatggc gcaggccacc ccgatctcgc
     1981 cttggcaacc gacctccgcc ccggaaatcg aggcgttggt cttggccaag ttgccgatca
     2041 atccggccgc ggccagggcc cgcaggatct ccctgtcgga aaaacggtgc tctttccgca
     2101 aatgagtgag aaccgccgga acgacgccgg ccgaaccgca ggtcggggcg gtgacgatca
     2161 cccctccggc cgcgttctcc tccgcgacgg ccagggcata ggcgaacagc agccccatcc
     2221 gcttcgaatg gccggcgaac atcttggcct tcatgaaata ggaccgggct ttgcgagaga
     2281 gacgaaggtc tcccggcagc gtgccctcgg tctttaatcc ccggcggacg gcctcggaca
     2341 tggccgccca aaccccggct aggaaatccc agatcccggg gccctcgacc tcctcgaccg
     2401 tctcccaaag cgtccggccg ttctgaacgc aggatcggac gatctcgtcc atcgagtgca
     2461 aggggtaaat ggaccggcgg acggtggccg gcgttccgtc gacgatctcg ccgccgccga
     2521 tgctgtaggc cgtccaaaat tcctggacgc ggccctcgtc atccaaggcc tcgaagcgga
     2581 gggcgttggg atggagggga aggcgttcgg ccgggcgcca aacgatctcg atcttccgtc
     2641 cgccgaactc ctcccggagc gtctcctccg tcaaatgccc cttgcccgtc gcggccaggc
     2701 tgccgtaaag ggtgacccgg taagccgggg ccgccgggac gcgggcgagg aacatgtcgg
     2761 cggccgcccg cggacccatc gtgtggctgg acgaagggcc gggcccgatg cgataaagct
     2821 cccggatcgt ttccatggat ttactatagc cgagcggggg cgggccgtcc aggggaagga
     2881 gcgcgttgac ggaccgcggg aacacgcgta ggatggaagg cttggccggg ccgggttccg
     2941 gcgatcatgc ggagcgaggt gcagctcatg cgcaagaaca gcgattccaa gcttattatc
     3001 ttggcggccg ccggatttct cgctttcgcg gccttcgccg ccgctcaaac agcgacggcg
     3061 cccgatccga aagccgtcgc ccttcgcggc gcctacggcc cctttcgggc gaataacgac
     3121 cttctgtcct atgatctcga catccgcgtc gatcccgagg ccaaggcctg gagcggtaaa
     3181 aacaccatcc ggttccggat gctcaaggag gatgcccgca tccagctcga tcttaaggcc
     3241 cacctgaccg tcgagaaaat caccctcgac ggcgctcccc tccccttcac ccgggaatac
     3301 gacgccgtct tcgtcgattt tccggagccg ctcaaaaagg attccgtcac ggcgatcgat
     3361 ttttacttct ccgggcgcgc ctccggcgaa aaggattggg gcggaatcac gttcaagacc
     3421 gatcccgccg gccgtccctg gatcacgacg gcctgccagg gatccggggc catgacctgg
     3481 tggccgaaca aggagcagca gcgcgacgag gtcgagcgca tgggcctccg ggtcgccgtt
     3541 cccaacggac tggtcgacgt ttccaacggc cgcttcgccg gccggaccga cctcggcgac
     3601 ggctataccc gctgggattg ggaggttcac taccccatca acaactacaa cgtcgccctg
     3661 aacatcgccg cttacgtcca tttcgaggac cggatggacg gcctgaccct ggattactac
     3721 tgcctcccgg agaacctgga aaaagccaag gtccagttcg cccaagcccg gacgacgatc
     3781 gaatgcttcg agaaatactt cggcccttac cccttccccg aggacggcta caagctgatc
     3841 gaggtccctt acgccgggat ggagcaccag tcggccgtgg cctacggcaa ccgcttcctc
     3901 aacggctacc tcgagaggga cttcaccggg gtcggcgtca gcttgaagtt cgactacatc
     3961 atcatccacg aatccgccca cgagtggttc ggcaattcca tcaccgcggc cgacgtctgc
     4021 gacgaatgga ttcatgaggc ctgggcgacg tacatggagg ggctctatgt cgaacacctg
     4081 ttcgggtacg aggacgcggt caagtacctg aacggctacc ggccgaaggt caagaacgag
     4141 aggcccatca tcggcccgcc ggacgtccac tacaacgcgc cgcaggacat gtatttcaag
     4201 ggggcgctgt ttctcaacac cctccggcac atcgtcgacg acgaggcgaa atggcggacg
     4261 cttctgcggg gattctacga ccgcttcaaa taccagaaca tcggctcgga ggacgccttc
     4321 gcctttctca acgtcgaaac gggtttgaac ctggggccgg tcttcgacca atacctgcgc
     4381 cggaccacca tcccggccct cgagctgaag ctcgacgagg cggcggggtc cgtgtccttc
     4441 cgctggaaat ccgaagtccc gggcttcgac atgccggtca aggccggacg gcgcgggcag
     4501 tggctgaccc tccggccctc ggccgaatgg cagacgatga aaacggatct gaaaaaagac
     4561 gaattcgagg tcgccgacga cctcttctat atcgacgtcg tccggatcga ttgagccgga
     4621 cggccccgtt ccggagcggg gaaaagaggg cctccccgcc ccccaagaag ccggacgctt
     4681 ccgcccttcc aaaagccgct ttcgtcatgt caggtggagc ggtcttccgc gacgatcgcc
     4741 agcgatgaaa aacggggccg gacgccggcg cctcaagccg cgcccggacg gcccggcccg
     4801 ccgagatgat tcacgacggc c
//
LOCUS       DAOOXH010000135         4804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_20598_length_4804_cov_8.111181, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000135 DAOOXH010000000
VERSION     DAOOXH010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20598_length_4804_cov_8.111181"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PKV64_10950"
     CDS             complement(<1..326)
                     /locus_tag="PKV64_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17633.1"
                     /translation="MKKHIAAVLCAGLLFGLAAASGIAAKDVIPKDIRKAMEAGLAES
                     QPTRTDIPFTFREPIIMPGPQQAVYAYLVMNIKNADLGYAPLPGTPKLQAEGHELGSS
                     RERGVS"
     gene            complement(653..1663)
                     /gene="mltG"
                     /locus_tag="PKV64_10955"
     CDS             complement(653..1663)
                     /gene="mltG"
                     /locus_tag="PKV64_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPN17634.1"
                     /translation="MRRIRLWLEIVLVAGMIGVLGGSAWLAWESRPPDGAGRETVLFE
                     IVPGQSVRSVARSLEARGLIRKSEPFVVLYRLHHGKETIKAGEFALPAGRGTKETLVT
                     LLRGKLYLHPVTVAEGLTAGETFDVFLAASFGARDAFEAGFRNTAEIALLDPGAADLE
                     GYLFPETYHFPKGAAAAEILSSMVAQFKNVFGPEERIRAAEIGMTPRQVVILASLIEK
                     ETGRPEEKPLVSAVFHNRLRIGMKLECDPTVIYALKREGKYNGRLLKKDLQFDSPYNT
                     YRYAGLPPGPICNPGEASIEAALAPAAADYLFFVADRERGHVFSRTLRDHQRAVHAYR
                     RK"
     gene            complement(1638..2078)
                     /gene="ruvX"
                     /locus_tag="PKV64_10960"
     CDS             complement(1638..2078)
                     /gene="ruvX"
                     /locus_tag="PKV64_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPN17635.1"
                     /translation="MRILGVDYGDRHIGLALSDPLGMIAQPLETYTLKEKKAENAAFF
                     RGLVQTHGIERIVLGLPLRMDGTSGTRAEKTQVFARWLEQNTGCPVVLWDERLTTRQA
                     TGIMQAQSVRIEKRRSVVNQIAAVLILQAFLDGQANDAPDSPVA"
     gene            complement(2075..3148)
                     /locus_tag="PKV64_10965"
     CDS             complement(2075..3148)
                     /locus_tag="PKV64_10965"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HPN17636.1"
                     /translation="MKSPLTVALFFGGRSAEHEVSITSARNVFDNLARSRYRRRCIFI
                     DKNGLWSEVSSPHLSASRLNRGPFHSFLPWDRSGRAKPLRADVYWPVLHGPGGEDGTI
                     QGLLELADVPFVGSGTLGSSMGMDKGVMKELFRSRGIPVVPLEVVTEPEAARSPAAVV
                     RRIRAGLKPPLFVKPANLGSSVGITKVKSWEELVPAMDEAFRYDRKILVEQGLDCREF
                     ECAVLGNDNPEASLLAEIIPGPKNEFYNYVEKYLENETRFVIPAVLPEAVAARARRLA
                     VAAFQACECSGMARVDFFRDKKSQRLYVNEINTIPGFTAISMYPRLWAASGLPFRALA
                     DRLIGLALERHRRKKRCLDYDSR"
     gene            complement(3204..3473)
                     /locus_tag="PKV64_10970"
     CDS             complement(3204..3473)
                     /locus_tag="PKV64_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPN17637.1"
                     /translation="MGKKEILARMSRDGGITLAQAQKAFASLLRGMRDSLKEGKKINL
                     SGFGSFEVKVREARKGRNPKTGTTIQIPQKKRIKFNPSRSFKNTL"
     gene            3636..4430
                     /locus_tag="PKV64_10975"
     CDS             3636..4430
                     /locus_tag="PKV64_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17638.1"
                     /translation="MMRFHRFTAAAAVGIFIGGIAGPGLGPAQDVLEKNAAVPKLISP
                     VDLDCSPFIMETAPQLRISVPLQGGERTLLADGDQFLVDPVPGVESFAEGSLWSILEW
                     GPKVRGERTSGPLGNAVFLRGRARVLRMEAGRAVMEVVKSCGAVTTGLLLVPYIKGDI
                     LTGAESSYDIPFREEGVPAGRVVFIESGWSDIVARGHWVLVDIGTDQGLAPGRQMTFY
                     REEADKTPRPIGNSVVVWTGPRWATLKILNSKDSILSGDLVQPKPD"
     gene            4501..4575
                     /locus_tag="PKV64_10980"
     tRNA            4501..4575
                     /locus_tag="PKV64_10980"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4533..4535,aa:Gly,seq:gcc)
     gene            4603..4674
                     /locus_tag="PKV64_10985"
     tRNA            4603..4674
                     /locus_tag="PKV64_10985"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4635..4637,aa:Cys,seq:gca)
BASE COUNT          883 a         1411 c         1551 g          959 t
ORIGIN      
        1 tgggatacgc cccgctcccg ggaactccca agctcatggc cctcggcctg gagcttggga
       61 gttcccggga gcggggcgta tcccagatcc gcgttcttga tgttcatcac gagataggca
      121 tagacggcct gctgcgggcc gggcatgatg atcggttccc tgaacgtgaa ggggatgtcc
      181 gttcgagtcg gctggctctc ggccaggccg gcttccatcg cctttcttat gtccttggga
      241 atgacgtcct tggcggcgat tccggacgcg gcggcgaggc cgaacaggag accggcgcag
      301 agaacagcag cgatatgttt tttcatcggg atcgtttctc ctctgaagga atttatcatt
      361 tgtcttccta attttatctg caatgttcat gccaagcgga attccagaga tcgcttcacc
      421 gccggctgcg ttaggcgttt cctattgtaa atattcgtta catcgccgga cggccggaga
      481 tctctgggag gaaacccgcc ggaccggcaa atatatcagg attccggatg cggatgcaaa
      541 tccgcgcatt gaatcatctc atgcgcagac gaaatccctg aaagagagga cggctgctga
      601 aggagatgga taaaggattg cggccacagc accgccggcc tgaatgggcc gattattttc
      661 ttcgataggc atggacggcg cgttgatgat ccctcaatgt ccggctgaag acatggcctc
      721 tttcgcggtc ggcgacgaaa aaaagataat ccgccgcggc cggggccaag gcggcctcga
      781 tggaggcttc gcccggattg cagatcgggc ccggcggcag gccggcatac cggtaggtgt
      841 tgtaggggga atcgaactgc aagtccttct tcagcagccg cccgttgtac ttcccctcgc
      901 gcttaagggc gtaaatgacc gtcggatcgc attcaagctt catcccgatc cggaggcggt
      961 tatggaagac ggccgagacc agcggctttt cctcgggccg gccggtttct ttttcgatca
     1021 gcgaagccag gatgacgact tgtcgcggag tcatcccgat ctccgccgcc cggatcctct
     1081 cttccggtcc gaagacgttt ttgaattgag cgaccatgct cgaaagaatt tcggcggcgg
     1141 ccgctccttt ggggaagtga taggtttccg ggaagaggta tccttccaga tcggccgcgc
     1201 ccgggtcaag cagggcgatt tcggcggtgt tccggaaccc cgcttcgaag gcgtccctgg
     1261 cgccgaacga ggcggcgaga aagacatcga acgtttcgcc ggcggtgagc ccttcggcca
     1321 cggtgaccgg atgaagatag agttttcccc gcaggagggt taccagcgtc tccttcgtcc
     1381 cccggccggc cgggagggcg aattcgcccg ccttgatggt ctccttccca tgatgcaacc
     1441 ggtagaggac gacgaagggc tcggatttcc ggatcaggcc ccgggcctcc agcgatcggg
     1501 cgacggagcg gacgctctgt cccggaacga tctcgaagag gacggtctcc cgtccggcgc
     1561 cgtccggcgg ccggctttcc caggcgagcc aggcgcttcc gccgagaact ccgatcatcc
     1621 ccgcgacgag gacgatctca agccacaggc gaatccggcg catcgttggc ctgtccgtcg
     1681 agaaacgcct ggaggatgag gacggcggcg atctggttga cgacggatcg ccgtttttcg
     1741 atccggacgc tctgggcctg catgatcccg gtggcctggc gggtcgtcaa gcgctcgtcc
     1801 cagaggacga ccggacaacc ggtgttctgc tcaagccagc gggcgaagac ctgggttttt
     1861 tcggcccggg taccggaggt gccgtccatc cgaaggggca gaccgaggac gatccgctcg
     1921 atcccgtggg tttggacgag accgcggaag aaggcggcgt tctcggcttt tttttccttg
     1981 agggtgtagg tttccaaggg ctgggcgatc atcccgaggg ggtcgctcag ggccaggccg
     2041 atatgccggt cgccgtagtc gacgccgaga atcctcatcg ggaatcgtag tccagacagc
     2101 gtttcttgcg gcggtgccgt tcgagggcca ggccgatgag ccggtcggcc aaggcccgga
     2161 acggaaggcc ggaggcggcc caaagccggg gatacatgct gatcgccgtg aaaccgggaa
     2221 tcgtgttgat ctcgttgacg tagagccgct gggatttctt gtcccggaaa aagtccaccc
     2281 tggccatgcc ggagcattcg caggcttgga acgcggccac ggccagccgc cgggcccggg
     2341 cggcgacggc ctcgggcagg acggccggga tcacgaaccg ggtttcgttc tcgagatatt
     2401 tttcgacgta gttgtaaaac tcgtttttgg ggccggggat gatctcggcc aggagggagg
     2461 cttcgggatt gtcgttgccc aggaccgcgc actcgaattc ccggcagtcg agcccctgct
     2521 cgaccaggat cttccggtcg tagcggaagg cctcgtccat ggccgggacc aactcctccc
     2581 agcttttcac cttggtgatg ccgacgctcg agccgagatt ggccggcttg acgaacagcg
     2641 gcggcttcaa tccggcccgg atccgccgga cgacggccgc gggcgagcgg gcggcctccg
     2701 gctccgtgac gacttcaagc ggaacgacgg gaatcccccg ggaccggaac aattccttca
     2761 tgaccccctt atccatcccc atggacgagc cgagcgtccc ggatccgacg aaaggaacat
     2821 cggccagctc gaggagcccc tggatcgttc catcctcccc tcccggcccg tggaggaccg
     2881 gccagtaaac gtcggcccgc agcggcttcg cccgtccgct cctgtcccaa gggagaaagg
     2941 aatgaaacgg cccgcggttg agccgggaag cggacaggtg gggggaggag acttcgctcc
     3001 agagcccgtt tttgtcgata aagatgcagc ggcgccggta gcgggagcgg gcgaggttgt
     3061 caaagacgtt ccgggcggac gtgatcgaaa cctcgtgttc cgcggaacgt ccgccgaaga
     3121 agagggcgac ggttaaggga gatttcatgg gcagtccgcc cccatcttaa aaaaaagcga
     3181 ggcgtttgtc tacgaggcgg gattcagagg gtgttcttga atgagcggct cggattgaac
     3241 ttgattctct ttttttgggg aatctggatc gtcgtcccgg ttttcgggtt ccgtcccttg
     3301 cgggcttcgc ggaccttgac ctcgaagctc ccaaaaccgg aaagattgat ctttttcccc
     3361 tctttcagag aatcccgcat ccctcggagg agtgaagcaa atgctttctg agcctgggcc
     3421 agggtgatcc ccccgtcgcg ggacatccgg gccaagattt ccttcttgcc cataggggca
     3481 cctcctctct tgctttccgt tgattgaatc atacgcacgg aaatgccgga ttgtcaagcg
     3541 attccgaaag tccgccgggg cgcgttgaca gccccaaaag ccgccgttat aatgaagata
     3601 agttgacagg ggcgcgagcc cggaaaggtc ggatcatgat gcgatttcat cgattcacgg
     3661 cggcggccgc ggttggaatt tttatcgggg ggatcgccgg acccgggctt gggccggccc
     3721 aggacgtcct cgagaaaaac gcggcggtcc ccaagctgat cagtccggtg gatttggatt
     3781 gttccccctt tatcatggaa acggccccgc agctccggat ctctgttccc ctccagggcg
     3841 gcgagcggac gctgctggcc gacggggatc aattcctcgt cgatccggtt cccggcgttg
     3901 aatccttcgc cgagggatcc ctatggtcca ttctggagtg gggaccgaaa gtccgggggg
     3961 aaagaacctc cggcccgctc ggaaacgccg tcttcctgcg cggccgggcg cgggttcttc
     4021 ggatggaggc cggtcgggcg gtgatggaag tcgtcaagtc ctgcggagcg gtcacgaccg
     4081 gattgctcct cgttccctac atcaaggggg acatcctgac gggagcggaa tcgagctacg
     4141 acattccctt ccgggaggag ggcgttccgg ccggccgggt cgtcttcatc gaaagcggct
     4201 ggtcggatat cgtcgctcgg ggacactggg tgctggtcga catcggaacg gaccagggac
     4261 tcgctcccgg caggcagatg acgttctacc gggaggaagc ggataaaacg ccgcggccga
     4321 tcggcaactc cgtcgtcgtt tggacggggc cccggtgggc gacgctcaag atcctcaatt
     4381 ccaaggattc gatcctgagc ggcgatctcg tccagccgaa gcccgattga caggttttcg
     4441 agtttctgtt agtttatcgc cgtgttgccc ggcttttcct cgccgggaaa ccgacgttgc
     4501 gcgggcgtag ttcagtggta gaacgtctgc ttgccatgca gaaggccgtg ggttcaagtc
     4561 ccatcgcccg ctccatccgc cgttaccggg cctcgtgaca acggcgcggt acccaagtgg
     4621 caagggagag gtctgcaaaa cctcgattcg ccggttcgaa tccggcccgc gcctttcttt
     4681 cccgctcctt ttccattgac accatctgcc gatctgcata tgatggattc cgcgatctga
     4741 acggaaagga ggagtcatga acctgatggc acgagtgcaa ggtatttgtc ttaaacccaa
     4801 ggag
//
LOCUS       DAOOXH010000136         4727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_21065_length_4727_cov_4.637842, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000136 DAOOXH010000000
VERSION     DAOOXH010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21065_length_4727_cov_4.637842"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PKV64_10990"
     CDS             <1..820
                     /locus_tag="PKV64_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17639.1"
                     /translation="HTGEEWAYDRIPFTTSSGQVVNLYSIKWLDYNEDGVIDGGDIHW
                     LGQSGNSTFQVRNMDQFDGQKARRMYMGYQLVFNKRLSNRWQMMASFLYSHSEGMANR
                     IFGNSMNAEGPMFTDNTWMGSLNYTINNMTGPLPFVPKFEFKISGSYTIPTVEVDLGA
                     RFRLHTGRPIWKLDEIPVHSEWSNPEGGIIGGGVGSIVSSVEPDYLPTQAILDLRAEK
                     SFVLPKFGTISVVLDAFNVFNADTPTTIDNQYSFGRVGSIVTPRTFRFSLMYQF"
     gene            954..3005
                     /locus_tag="PKV64_10995"
     CDS             954..3005
                     /locus_tag="PKV64_10995"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPN17640.1"
                     /translation="MSRIGAVLLAIGMAALPAAGQVAKSAVLPEKYRIWLDEEAVFLI
                     TPTERDVFLKLDSDRSREIFIEAFWKQRDPVPETPRNEFREEHYRRLQYANRMYGRGS
                     RLPGWKTDRGKIYIVLGPPRSIESFDDINNVHPTEIWFYQDTGFPTLPVAFNIIFFKR
                     NGFGDYVLYSPAADGPRSLIATAMGDARDEQAYRDLRELAPNLASQVLSLIPGERRPS
                     RTPSLSSETLLADIFDSPRRKIKDAYAEALLRYKDAVEVEYTANYIDVGALLYVSRSG
                     DGTSLIHYAVSPARLTLASSGGRNEAFFSFNGRLTDVDGRTVYQFEKSFPLSFNDADV
                     GEIRATSIVFQDVFPCVPGRFRFDLLLKNTVSKEFGSFEAAIEIPDPGAGPAVGSLFL
                     GYSAETAAFGPAEAVPFRIGGRQLLGDVRNRFVAADTMRIVFQAFGLPEAWTAGGRIR
                     ADVLKDSVPVLSQTRTPAEAGAGETFFLEQSLRELKPGYYDLEVTLLDGAGRELARRG
                     APFEVGLPGRLARPRVLSGVTGADYAEEWDYALGLQYLNLGKSSEAGRAMARAYEKHP
                     ENERFTSGYSQALYLLGESRKAKDVLLPLAEDENAAPEVLAGLGRACQSLGEFREAIA
                     HYRRYLARAGTNIEILNYIGTCHYQLGEKDAALDVWNQSLKINPRQERLKALVDSLAG
                     K"
     gene            complement(2950..3852)
                     /locus_tag="PKV64_11000"
     CDS             complement(2950..3852)
                     /locus_tag="PKV64_11000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPN17641.1"
                     /translation="MNGPPLRPRTMSGRRVLALAVLALAAGGIPAGGCGRRPPRNTAP
                     DPCLVFRCEEGGIIRGRTDRREMALIFTGGDFADGGEAIRGVLREKRVSAGFFFTGDF
                     YRSASNTALIRGFIADGHYLGPHSDKHLLYCSWEDRARTLVTREEFAADLRANYEIME
                     TFGLRRKDAPFFIPPYEWYNRDISGWARSLGLVLFNFTPGTSSNADYTTPDMPEYLSS
                     REIWDRIWEAEGRDPDGFNGFILLVHIGTDPRRTDKFYARLDRLIDGLEAKGYRLVRI
                     DRLLREAISPPGNPPGPSAAPAGD"
     gene            complement(3849..>4727)
                     /locus_tag="PKV64_11005"
     CDS             complement(3849..>4727)
                     /locus_tag="PKV64_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPN17642.1"
                     /translation="AAEREEGGGMSGPAVVGIDLGGTNVRAGIVKGQRLIRVAASAVP
                     ADRPREEVLEAFFALADQVIDRSIEAIGVGVPSVIDLKAGVLYDVQNIPAWKEVPLKA
                     LIEKRYGIPAYINNDANCFAVGEKYFGLAKPYDHAVGLIVGTGLGAGLIANGRLYAGS
                     NCGAGEFGMVPYRDGTLEDYASGRFFKTAYGTSGRELFDRALCGDLTARAVFREFGTH
                     LAEAVKVILFAVDPEIIVLGGSVSKAFAYFEQTLRDSLSNFPYSITIRNLKIDVSTTE
                     HIAILGAAALIFDARK"
BASE COUNT          848 a         1535 c         1491 g          853 t
ORIGIN      
        1 ccataccggc gaggaatggg catacgaccg gatccccttc acgacctcct cgggccaagt
       61 cgtcaatctc tacagcatca agtggctcga ttataacgag gacggggtta tcgacggagg
      121 cgacatccac tggctcggac agtccgggaa ctcgaccttc caggtccgga acatggacca
      181 gttcgacggc cagaaagccc gccggatgta catgggctac cagctcgttt tcaacaagcg
      241 gctgtccaac cgctggcaga tgatggcgtc cttcctctac tcgcactcgg aaggaatggc
      301 caaccggatt ttcggcaaca gtatgaacgc cgagggcccg atgttcaccg acaacacctg
      361 gatgggttcg ctcaactaca ccatcaacaa catgaccggc cccctgccct tcgtgccgaa
      421 gttcgaattc aagatctccg gatcgtacac gatccccacc gtggaggtcg acttgggcgc
      481 ccgcttccgg ctccacacgg gccgccccat ctggaagctt gatgagatcc cggtccattc
      541 cgagtggtcc aatcccgaag gcggaatcat cggagggggc gtgggctcga tcgtttccag
      601 cgtcgaaccc gattaccttc cgacccaagc cattctcgac ctgagagcgg aaaagtcgtt
      661 cgtgctgccc aagttcggga cgatctcggt cgttctcgac gcgttcaacg tcttcaacgc
      721 ggacacgccg accacgatcg acaaccagta ttcgttcggg cgcgtcggat cgatcgtcac
      781 gccgcgcact ttccgcttca gcctcatgta ccagttctag gagccgttcg actcggcgcc
      841 gcacggccgc cagtccgccc ggagggcccc ccggccctcc gggctttttt tcagcccggg
      901 cggggcgttg tccgccgccg gagaatcgga tatcatctga ctcgaggtga agcatgtccc
      961 gcatcggcgc cgtactcctg gcgatcggga tggccgcgct cccggccgcc ggccaggtcg
     1021 cgaaatcggc cgttcttccg gagaagtacc ggatctggct cgatgaagaa gccgtcttcc
     1081 tcatcacccc cacggagcgg gacgtctttc tcaagctcga ctccgaccgg agccgcgaaa
     1141 tcttcatcga ggccttctgg aagcagcgcg accccgtgcc ggaaacgccg cgcaacgagt
     1201 tccgggagga gcattaccgc cgcctccagt acgccaaccg gatgtacggc cgcggctccc
     1261 gccttccggg ctggaagacg gaccggggaa aaatctatat cgttctcggc ccgccgagga
     1321 gcatcgagtc gttcgacgac atcaacaacg tccatccgac ggagatctgg ttctaccagg
     1381 atacgggatt cccgacgctg ccggtggcct tcaacattat ttttttcaag cgcaacggct
     1441 tcggtgatta tgtcctgtat tccccggcgg ccgacggccc ccgcagcctg atcgcgacgg
     1501 ccatgggcga tgcccgggat gagcaggctt accgggatct ccgcgagctg gcccccaacc
     1561 tggcttccca ggtcctctcc ttgatccccg gcgaacgccg tccgtcccgg acgccgtcgc
     1621 tgtcgtcgga gacgcttctg gcggatatct tcgactcgcc ccgccgaaaa atcaaggatg
     1681 cctatgccga ggccctcctc cgctacaagg acgccgtcga ggtcgaatac acggccaatt
     1741 acatcgatgt cggggcgctt ctctacgtct cccgaagcgg cgacggcacg tccctgatcc
     1801 attacgcggt ctcgcccgcc cgcttgacgc tcgcgtcctc cggcggccgg aacgaggcgt
     1861 ttttctcctt caacggccgg ctgaccgacg tggacggccg gaccgtctac caattcgaaa
     1921 aatcgtttcc cctctcgttc aacgacgccg atgtcggcga gatccgggcc acgtcgatcg
     1981 tcttccagga cgtttttccg tgcgtcccgg ggcgtttccg attcgatctc ctgctcaaga
     2041 acaccgtttc caaggaattc ggctcatttg aggcggcgat cgagatcccg gaccccgggg
     2101 ccgggccggc ggtgggcagc ctgttcctgg gctattcagc cgaaaccgcc gccttcggcc
     2161 cggccgaggc ggtgcctttc cgaatcgggg gacggcagct cctcggcgac gtccgaaacc
     2221 gttttgtcgc cgcggacacg atgcggatcg tcttccaggc gttcggcctt ccggaggctt
     2281 ggacggccgg gggacggatt cgagccgacg ttctcaaaga cagcgtcccg gtcttgtccc
     2341 aaacccggac gccggctgaa gccggagccg gggaaacgtt ctttctcgaa caatccctcc
     2401 gggagctcaa gcccggctac tatgaccttg aagtcacgct ccttgacggg gcggggcggg
     2461 aactcgcccg ccggggcgcg ccgtttgaag tcggccttcc cgggcggctg gcgagaccac
     2521 gggtcctgtc cggcgtaacc ggcgcggatt acgcggagga gtgggactac gccttgggtc
     2581 ttcagtattt gaacttggga aaatccagcg aggcgggacg ggcgatggcc cgggcctatg
     2641 agaaacatcc cgaaaacgag cggttcacct ccggctattc tcaagcgctc tatctcctgg
     2701 gggagtcccg gaaggccaag gatgtgctcc ttcccctggc cgaagacgag aacgccgctc
     2761 ccgaggtgct ggccggactc ggccgcgcct gccagtcgct gggggaattc cgggaagcga
     2821 tcgcccatta ccgccgttac ctggcccggg ccgggacaaa catcgagatc ctcaattata
     2881 tcgggacctg tcattaccag ttgggggaga aggacgccgc cctggatgtt tggaatcagt
     2941 cgctgaagat caatccccgg caggagcggc tgaaggccct ggtggattcc ctggcgggga
     3001 aatagcctct cggagaagcc ggtcgatccg gacgagccgg taccctttgg cctcgaggcc
     3061 gtcgatgaga cggtccagcc gggcatagaa tttgtccgtc cgcctcggat cggtcccgat
     3121 gtgaaccagg aggatgaatc cgttgaatcc gtccgggtcc cgcccttcgg cctcccagat
     3181 ccggtcccag atctcccgcg aggacaagta ttcgggcatg tccggagtcg tgtaatcggc
     3241 gttggaggac gtcccgggcg tgaaattgaa aaggacgagc ccgagggagc gggcccagcc
     3301 cgaaatgtcc cggttgtacc attcgtaggg cgggatgaaa aagggcgcgt ctttccggcg
     3361 cagtccgaac gtttccatga tctcgtaatt ggcccgcagg tcggcggcga attcctcccg
     3421 ggtcacgagc gtccgggccc ggtcctccca ggagcagtag aggagatgct tgtcggaatg
     3481 cggccccaga taatgtccgt cggcgatgaa gccgcggatg agggccgtgt tcgaagcgga
     3541 ccggtagaaa tcgccggtga agaaaaaccc ggcggagacg cgcttctccc ggagaacgcc
     3601 gcggatggct tcgcctccgt cggcgaagtc gccgccggtg aagatcaagg ccatctcccg
     3661 gcggtccgtc cggccgcgga tgattcctcc ctcctcgcag cggaagacga ggcagggatc
     3721 cggggccgtg ttccgcggcg gcctccggcc gcaccctccg gccgggattc ctcccgccgc
     3781 cagggcgagg acggccaggg cgaggacgcg ccggccggac atcgtccgcg gccggagcgg
     3841 gggtccgttc atttccgcgc gtcgaagatc agggcggccg cgcccaggat ggcgatgtgc
     3901 tcggtcgtcg agacgtcgat cttgaggttg cggatcgtga tcgagtaggg aaaattgctc
     3961 aaggaatccc gaagggtttg ctcgaagtag gcgaacgcct tggacacgga tcccccgagg
     4021 acgatgatct ccggatcgac ggcaaagagg atgaccttga ccgcttcggc taaatgcgtc
     4081 ccgaattccc gaaagacggc ccgggcggtc aagtcgccgc agagggcccg gtcgaaaagc
     4141 tcccggcccg atgtcccgta agcggtcttg aaaaaacggc cgctggcgta atcctcgaga
     4201 gttccatccc ggtaggggac cattccgaac tcgcccgccc cgcagttgga tccggcgtag
     4261 agccggccgt tggcgatgag gccggccccc aggccggtgc cgacgatcaa gccgacggcg
     4321 tggtcgtagg gcttggccag gccgaaatat ttctcgccca cggcgaagca gttggcgtcg
     4381 ttgttgatgt aggccggaat cccgtagcgc ttctcgatca aggccttgag ggggacttcc
     4441 ttccaggccg gaatgttttg gacgtcgtag aggacgcccg ccttcagatc gatgacgctg
     4501 ggaacgccga cgccgatggc ctcgatggac cggtcgatga cttggtcggc gagggcgaaa
     4561 aaagcctcca ggacttcctc ccggggacgg tcggccggaa cggccgaggc cgcgacccgg
     4621 atcagccgtt ggcccttgac gattccggcc cggacgttgg tgccgccgag atcgatgccg
     4681 acgacggccg ggccgctcat gccgcctcct tcctctcttt ccgccgc
//
LOCUS       DAOOXH010000137         4647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_21594_length_4647_cov_6.540723, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000137 DAOOXH010000000
VERSION     DAOOXH010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21594_length_4647_cov_6.540723"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..769)
                     /locus_tag="PKV64_11010"
     CDS             complement(149..769)
                     /locus_tag="PKV64_11010"
                     /inference="COORDINATES: protein motif:HMM:NF016163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HPN17643.1"
                     /translation="MRPLVVLGLDLAGSPRRPTGICVLRALGAETEILFDDARIEALA
                     ARVRPDLIVVDAPLNLPPGRASFADRNGEHFRPCDRELRARGIPFFPITLGPMRSLTL
                     RGIALKERLEKRGFRVLEMYPGGAQDLWGIPRAKRDAAGLRRGLVRLGLRGLGRKLCA
                     HELDAATGALVGRLFLQGKAGVYGDFRTGAILMPRDRPGRATGKRS"
     gene            complement(766..1176)
                     /locus_tag="PKV64_11015"
     CDS             complement(766..1176)
                     /locus_tag="PKV64_11015"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPN17644.1"
                     /translation="MSDNEFKVTFPGGVRVDAAYGGFTIRTDQPVRDGGEGSAPSPFV
                     LFLASLATCAGYYVLSFCRERNIPASGIELTMRTERDAEGKMISLASITISLPPDFPE
                     KYAGAVVKSAERCTVKAHLQFPPDIETSAVIRKA"
     gene            complement(1178..1921)
                     /locus_tag="PKV64_11020"
     CDS             complement(1178..1921)
                     /locus_tag="PKV64_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPN17645.1"
                     /translation="MKNALPELAVVLVRPENPENIGLAARAMKNTGFAELRLVGVRRP
                     GPAAIKTAVHAGDILAGALLFPTLEEATDDLHFIAASTAKRRKIETIVDWDEAAGRIR
                     RLPEGTRAGLLFGNERTGLTGAELRRANLIFTIPQAARQPSYNLAAAVLLTLFTLFDG
                     AREEKRAAPPAGGEDARTPGLPRREQDETIGRIIAQLSARGFIHRTNRDHVSAWIHDY
                     FGRTAMTARDRGFLLALFADKRSTQKKES"
     gene            2118..3464
                     /locus_tag="PKV64_11025"
     CDS             2118..3464
                     /locus_tag="PKV64_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HPN17646.1"
                     /translation="MWWEEKTMRTFRCRLRQQPGVLGRLLSAIGAAGGDIGEIRVLRQ
                     GTTHVERDIVVFALGPEAMDRLIAAAGSVPDVELLEVRDDVLEMHQGGKIAIRSRYKI
                     DSLEVLRRVYTPGVAEVCLQIRDHPRLARRFTSIRHFVAIVTDGTAVLGLGDIGPQAA
                     MPVMEGKASLMETFCGLSGVPILLDTKDVDDIVDTVAAIAPTFSAIQLEDISAPRCFD
                     IEARLQDRVDIPVLHDDQHGTAAVVVAALLNAAKAARRPLHESRIGVVGLGAAGMAIG
                     KMLMFTTGRPVLGADLSGEALRRFEGYGGTASALKEIMAVCDVVVTVTGAAGLVKPAM
                     IRPGQIVLSLSNPVPEIDPAAALKAGAAFAADGKAVNNVLGFPGIFRGAVDAAVARIT
                     EDMLLAASRAIADAAPAGEIVPSPLDRGLHRSVARAVARVALEKGLNRDDLTGYFD"
     gene            complement(3676..4029)
                     /locus_tag="PKV64_11030"
     CDS             complement(3676..4029)
                     /locus_tag="PKV64_11030"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HPN17647.1"
                     /translation="MIDRLTCLLCPLGCELEVETAGGRLEVRGHQCDKGLDFASEEVL
                     RPKRNLAASIPVAGTDGRTTAVRLSGRVPREMIFPVLAEIAKLRPAIPVRRGDVLIRD
                     VLGTGVDVIATRTVV"
     gene            complement(4022..>4647)
                     /locus_tag="PKV64_11035"
     CDS             complement(4022..>4647)
                     /locus_tag="PKV64_11035"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPN17648.1"
                     /translation="PLELSTTVIDIRGRERVSSVVVARVDDRLKPLAGTEREIACDTL
                     LLSVGLIPENELSRRAGVVLSPATGGAVVDETFMTTVPGIFSCGNVLQIHDVADGASL
                     EGFEAGKNAARFARGAVAAREAPAGIAAGAGIKYVLPQIVRRGTAGAGLHFRVAEPRR
                     NVWIEVCGRSSGTTLFRRKYPRLLPSELQRIVVKAAIVEDLEVSAHD"
BASE COUNT          792 a         1540 c         1583 g          732 t
ORIGIN      
        1 tgcatgccca gtacaacggg gaagcgatcg aaaaacgcga atccttcgag atgaacgtga
       61 tccataaagc cctccgggtg atcggacccc ggaccgtgga taaaaagcgg ccgaaccgga
      121 cggagtggat cgccggagat tccggctttt agcttcgctt tccggttgcc cgccccggcc
      181 ggtcccgagg catcaggatg gcccccgtcc ggaaatcgcc gtagacgccg gctttcccct
      241 ggaggaacaa gcggccgacg agcgccccgg tcgccgcatc gagctcgtga gcgcagagct
      301 tccgtccgag cccgcgcagg ccgaggcgga caagcccccg gcgaagcccg gccgcgtccc
      361 gcttcgcccg gggaatcccc caaagatcct gggctccccc gggatacatc tcgaggacgc
      421 ggaatcccct cttctcgaga cgttccttga gagcgatgcc gcgaagagtc aaggaccgca
      481 tcgggccgag ggtgatgggg aaaaacggga tgccccgggc ccgaagctcg cggtcgcagg
      541 gacggaaatg ctcgccgttc cggtccgcaa acgacgcccg gccgggcggc aggttcagag
      601 gagcgtcgac gacgatgagg tcgggtcgga cccgcgcggc caaggcctcg attcgggcgt
      661 cgtcgaacaa gatctccgtt tccgccccga gggcgcggag gacgcaaatc ccggtcggcc
      721 gccgggggga tccggccaag tcgaggccga ggacgacgag cggtctcacg ccttccggat
      781 gacggcgctc gtctcgatgt ccggcgggaa ttggaggtgg gctttcaccg tgcaccgctc
      841 ggccgacttg acgacggccc cggcgtattt ctccgggaaa tccggaggga ggctgatcgt
      901 gatggacgcc aggctgatca ttttcccctc ggcgtcgcgc tcggtccgca tcgtcagctc
      961 gatgcccgac gcggggatgt tccgctcccg gcagaacgaa agcacgtaat acccggcgca
     1021 ggtcgcgagc gaggctaaaa aaagcacgaa gggcgagggc gcggatccct ccccgccgtc
     1081 gcggacgggt tggtccgtgc ggatcgtgaa tcctccgtaa gcggcatcga cccggacgcc
     1141 cccgggaaac gtgaccttga actcgttgtc ggacatctca agactccttt ttttgggtgg
     1201 aacgcttgtc ggcgaagagg gcgaggagaa agccccggtc ccgggcggtc atcgccgtcc
     1261 ggccgaaata atcatgaatc caggcggaga catgatcccg gttcgtccgg tggatgaagc
     1321 cgcgggcgga gagctgggcg atgatccggc cgattgtctc gtcctgctcc cgccggggaa
     1381 ggccgggcgt tcgagcgtct tcgccgccgg ccggcggcgc ggcccgcttc tcttcccggg
     1441 ctccgtcaaa gagggtgaag agcgtgagga gaacggccgc ggccaggttg taggacggct
     1501 ggcgggcggc ctgggggatc gtgaagatca ggttggcccg gcgcagctcc gcgccggtca
     1561 aacccgtccg ttcgtttccg aacaggagac ccgcccgggt tccctccggc aggcggcgga
     1621 tccgtccggc ggcctcgtcc cagtccacga tggtttcgat cttccgccgt ttcgccgtgg
     1681 acgcggcaat gaagtgaaga tcgtcggtcg cctcctcgag cgtcgggaac aggagggccc
     1741 cggccaggat gtcgccggca tgaaccgcgg tttttatcgc cgccggaccc ggccgccgga
     1801 cgccgacaag gcggagctcg gcgaatccgg tgtttttcat cgcccgggcg gccagaccga
     1861 tgttttcggg gttttccgga cggacgagga cgacggccag ctcgggaagc gcgtttttca
     1921 tggtcgccgg acgtcctttc gggttcccat tatatgctat aatgacgccg atttccgatc
     1981 ccggatcttc acggagcgtt ccgggagtct cccggcggca aagggaataa ccccgtcccg
     2041 gcggtcccgc gcccggggat ttcttcggcc tcccgatttc cttgatcttt cgaccgcggg
     2101 acataaggag ggaaccgatg tggtgggaag aaaaaacgat gcggacgttc cgctgccggc
     2161 tccggcagca gccgggggtc ctcggccggc tgctctcggc gatcggcgcg gccggcggtg
     2221 atatcggaga aatccgcgtt ctccgccagg ggacgacgca cgtcgagagg gacatcgtcg
     2281 tcttcgccct cggcccggag gcgatggacc ggctgatcgc cgcggccgga tccgttcccg
     2341 acgtcgagct tctcgaggtc cgcgacgacg tcctggagat gcaccagggc gggaagatcg
     2401 ccatccgcag ccgctacaag atcgacagcc tcgaggtcct tcggcgcgtt tacacgccgg
     2461 gagtggccga ggtctgcctc cagatccggg accatccccg gcttgcccgc cggttcacgt
     2521 ccatccgcca tttcgtggcg atcgtcaccg acggaacggc cgtcctgggc ctgggcgaca
     2581 tcgggcctca ggcggccatg ccggtcatgg aaggcaaggc cagcctgatg gagacgttct
     2641 gcgggctgtc cggcgtcccg atccttctcg acacgaagga cgtcgatgac atcgtcgaca
     2701 ccgtggccgc gatcgccccg acgttttcgg ccattcagct agaggacatc tccgccccgc
     2761 ggtgcttcga catcgaggcg aggctccagg accgggtcga cattccggtc ctccacgacg
     2821 accagcacgg aacggccgcg gtcgtcgtcg ccgccctgct caacgcggcc aaagcggccc
     2881 gccggccgct ccacgaaagc cgaatcggcg tcgtcgggct gggcgcggcc gggatggcca
     2941 tcggcaagat gctgatgttc acgacgggga ggccggttct gggggccgac ctttccggcg
     3001 aagccctccg ccggttcgag ggatacggcg ggacggcgtc cgcgctcaag gaaatcatgg
     3061 ccgtctgcga tgtcgtcgtc acggtgaccg gggccgcagg cttggtcaag ccggccatga
     3121 tccggcccgg gcagatcgtt ctctccttgt ccaacccggt tccggaaatc gatccggccg
     3181 ccgccttgaa ggccggagcg gccttcgccg ccgacggcaa ggccgtcaac aacgtcctgg
     3241 gctttccagg gatttttcgg ggggccgtgg acgccgccgt cgcccgcatc accgaagaca
     3301 tgctcctggc ggcctctcgg gcgatcgccg acgccgcgcc cgcgggcgag atcgtcccca
     3361 gtccgctcga ccggggcctc caccggagcg tcgcccgggc cgtggcccgg gtcgccctgg
     3421 aaaagggctt gaaccgcgac gacttgaccg gatatttcga ttgattgaac cggcccgccg
     3481 gcctcgcgag tcgagaggaa aacgggtgcg gggggcggac gaagccgtcc tgatcagatc
     3541 gcgagccccg cgcaaccaag cagatgcggt tccatcgacc cgccagaaga taagaactca
     3601 tgattaattt tgcgaggatg gagatcaatt cgactgttta aaaaggagac gcgtctgcaa
     3661 accaaaggcg ccggctcaaa caacggtccg ggtggcgatg acgtcgacgc ccgttccgag
     3721 aacgtcgcgg atcaggacgt ctccccggcg gaccgggatg gcgggacgca gtttcgcgat
     3781 ttcagccagg acggggaaga tcatctcccg ggggacgcgg ccgctcaaac ggacggcggt
     3841 cgtccggccg tccgttccgg ccacggggat ggaggcggcc aagttccgct tcggccgaag
     3901 gacttcctcc gaggcgaaat cgagtccttt gtcgcactgg tggccgcgga cttcaagacg
     3961 gccgccggcg gtctcgactt caagctcgca gccgagcggg cagagaaggc acgtcagccg
     4021 gtcaatcatg ggccgaaacc tccaggtcct ccacgatcgc ggccttcacc acgatccgct
     4081 gaagctccga gggaaggagg cggggatatt ttctccggaa cagcgtcgtt ccgctcgacc
     4141 ggccgcagac ctcgatccaa acgttccggc gcggctccgc gacccggaaa tgaaggccgg
     4201 ctccggccgt tccgcgccgg acgatctgcg gcaggacata cttgatcccc gctccggccg
     4261 cgatcccggc cggagcctcc cgggccgcga cggccccccg ggcgaaccgg gcggcattct
     4321 tccccgcttc gaacccctcg agggaagccc cgtcggccac gtcgtggatc tggaggacgt
     4381 tgccgcagga aaaaatcccg ggaaccgtgg tcatgaacgt ctcatcgacg accgcgcctc
     4441 ccgtcgccgg gctgaggacg acgccggccc gccgggacag ctcgttttcg ggaatgagcc
     4501 cgaccgaaag aagaagcgtg tcgcaggcga tctcccgctc cgtcccggcc aagggcttga
     4561 ggcggtcatc gacccgggcg acgaccacgg acgagacgcg ctcccgcccg cggatgtcga
     4621 tgaccgtcgt cgaaagctcg aggggga
//
LOCUS       DAOOXH010000138         4539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_22315_length_4539_cov_7.250223, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000138 DAOOXH010000000
VERSION     DAOOXH010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22315_length_4539_cov_7.250223"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="PKV64_11040"
     CDS             complement(<1..915)
                     /locus_tag="PKV64_11040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPN17649.1"
                     /translation="MATSLLVETNCIYFKGEKPCVYKRTCDHCPHFKPFGPKILVIKC
                     RAQGDVLRTTPLLTGLKRQYSEAFITWLVDEETVDLLRGIPLIDRVVPLNAETLLALR
                     AQRFDAVYSLDKDPGLTALAVLLDAPRKLGFGLNEHGNLTALTPAAAYSLRLGVDDDL
                     KFRVNRMTYQEAIFEAAEIEYQADDYVFVLADAPRQKAERFFLDRKVPKKRLNIGLNT
                     GAGAKFTTKQWPEDHFLELIPKLKRALNANIFLLGGPREKALNQRLARRSKLKVFDTG
                     TDNTLLEFAGFLAKMDVVVCSDTLAMHLA"
     gene            complement(1023..1700)
                     /locus_tag="PKV64_11045"
     CDS             complement(1023..1700)
                     /locus_tag="PKV64_11045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPN17650.1"
                     /translation="MTPVSASLRPVRVTRRAVYGLFRGAGFPMKLYPRLKFRMCPILR
                     IEAYVPDGCDILDLGCGNGLFTAILKLGSPDRKIIGIDLDARKITAARRFLGKLRGVD
                     FRLGNVVSAPYPKTGACTIIDVLYLLPVETQNAILKKCAGALPSGGTLILKEMDLAPR
                     WKYAWNAVQETISVKIVGFTLGSRFHFRGREDYAATLAGLGFDVEIIPLHRGYAYSHI
                     LYVARKK"
     gene            complement(1710..>2242)
                     /locus_tag="PKV64_11050"
     CDS             complement(1710..>2242)
                     /locus_tag="PKV64_11050"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HPN17651.1"
                     /translation="NAALAEDPYAPVAFHAANTRAADPLDRIAYLDLKLYLQDDLLPK
                     VDRMSMAASLEIRAPFLDYTFVEFAAAIPSSLKLRGFRSKDILKKALAGRLPESILNK
                     KKIGFDIPLGVWMRNGLKSFVLETLAPDRLRRHGFFDQGFVDSILKEHFAGSHNHRQL
                     LWPLIIFQNWYDRYGR"
     assembly_gap    2243..2342
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2343..3760)
                     /gene="asnB"
                     /locus_tag="PKV64_11055"
     CDS             complement(<2343..3760)
                     /gene="asnB"
                     /locus_tag="PKV64_11055"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HPN17652.1"
                     /translation="MNAMCGICGFVDGRGLDPGAPDLLKRMTRTLIHRGPDDEGFHID
                     GQAALGMRRLSIIDLVTGHQPMANEDRTVWVVMNGEIYNFPEIRKDLLARGHVLATQS
                     DTEVVVHLYEERGTDFVHALNGMFAVVLWDAKSRRLVLARDRLGVKPLHYCRIGSRLY
                     FASEIKALLQAPIPRDIDLESLARFFTFEYIPAPRSIFRGIRKILPGRLLVFEDGKET
                     ELSYWDVRHAETAIVPRPAAEYTAEIIDRLRESVRRRLISDVPLGVFLSGGIDSSAVT
                     ALMSEVAGAGIKTYSIGFREKTFDELDAARIVAGKFGTDHRAFVVTPAEVSGLVPTLA
                     GFLDEPLADASIIPTYVISKLAREYVTVALAGDGGDELFAGYDTYKAYKAARWYRKVP
                     GFARRGLIRPVVRALPASARRLSFEFKAKKFVSGAEYPPEIANFIWWGAYPPELRRRL
                     FAPPINAALAEDPYAPVAFHAA"
     gene            3841..4512
                     /locus_tag="PKV64_11060"
     CDS             3841..4512
                     /locus_tag="PKV64_11060"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HPN17653.1"
                     /translation="MKKIIINADDFGLCAPVNEGIILAHRRGILTSATLMTNTPGFEQ
                     AVALARENPALGVGVHLNIVRGRPLSPPEEIPTLVGPDGRFPAGAGRILLKLAAGRIR
                     PAEIERELRAQIEKARATGLPLTHLDSEKHMHAVPAVFKIVLRLGREYGFRKVRFIRE
                     YGLSRHPAQSLKAAFLSLCCARMKRRVRAAGFVITDSFYGICNSGRMSAARLRRIYIY
                     HKTNR"
BASE COUNT          805 a         1301 c         1544 g          789 t
ORIGIN      
        1 ggccaggtgc atggccagcg tgtccgagca gacgacgacg tccatcttcg ccaggaagcc
       61 ggcgaattcg aggagggtgt tgtccgtgcc ggtgtcgaag accttgagct tgctccggcg
      121 ggccagccgc tggttgaggg ctttctcgcg cgggccgccg aggaggaaga tgttggcgtt
      181 gagggcccgc ttcagcttcg ggatgagttc caggaaatgg tcctcgggcc attgtttggt
      241 cgtgaacttc gccccggcgc ccgtgttcaa gccgatgttg agccgctttt tcggcacttt
      301 gcggtcgagg aaaaagcgct cggctttttg acgcggggcg tcggccagaa cgaagacgta
      361 atcgtcggcc tggtactcga tctcggccgc ctcgaagatg gcctcttgat aggtcattcg
      421 attgacccgg aacttgaggt cgtcgtcgac gccgagccgg agggaatagg cggccgccgg
      481 ggtgagggcc gtgaggttgc cgtgttcgtt caggccgaag ccgagcttgc ggggcgcgtc
      541 cagcagaacg gccagggcgg tcaggcccgg atccttgtcc aatgaataga ccgcgtcgaa
      601 ccgctgggcc cggagggcga gaagggtttc cgcgttcaag gggacgaccc ggtcgatcag
      661 ggggatgccg cggagaaggt cgacggtttc ctcgtccacc agccaggtga tgaacgcctc
      721 ggaatactgc cgcttcaggc ccgtcaggag gggggtcgtc cggagaacgt ccccttgggc
      781 ccggcacttg atgaccagga ttttgggccc gaagggcttg aaatgggggc agtggtcgca
      841 tgtccgctta taaacgcagg gtttttcgcc cttgaaatag atgcaattcg tctcgacgag
      901 cagggaggtt gccatcagcc gtatgttatg ccatatcgcc gggtttgtcg caagtcagcc
      961 gatccgggcc gccggccgtt cctttcaagg atcgccggcc tggcttgaag tcccccgaat
     1021 ccctattttt tccgggcgac gtagaggatg tgggagtagg cgtagccacg gtggaggggg
     1081 atgatctcca cgtcgaatcc caggccggcg agggtggcgg cataatcctc ccggccccgg
     1141 aaatggaacc gcgagccgag ggtgaagccg acgatcttga cggagattgt ctcctggacc
     1201 gcgttccaag catatttcca gcggggcgcg aggtccattt ccttcaggat cagcgttccc
     1261 ccggagggca gggccccggc gcacttcttt aatatcgcgt tctgagtctc gaccggcagg
     1321 aggtagagga cgtcgatgat tgtgcaagcg ccggttttgg ggtaaggggc ggaaacgacg
     1381 ttgccgagcc ggaaatccac gccgcgaagc ttccccagga accgccgggc ggccgtgatt
     1441 ttccgggcgt cgagatcgat tccgatgatc ttccggtccg gggaaccaag cttcaggatg
     1501 gccgtgaaca agccgttccc gcagccgagg tctaaaatgt cgcacccgtc cgggacgtag
     1561 gcttcgatcc ggagaatcgg acacatgcgg aacttaaggc gcggatagag cttcatcggg
     1621 aaaccggcgc cgcgaaagag gccgtagacg gctcttcggg tgacgcggac ggggcggagc
     1681 gaggcggaca caggagtcac gattgatcct tatcggccat agcggtcgta ccagttctgg
     1741 aagatgatca gcggccacag cagctggcgg tggttgtgcg agccggcgaa atgctccttc
     1801 aatatcgaat cgacgaagcc ctggtcgaaa aaaccatgcc ggcgaaggcg gtcgggggcg
     1861 agggtctcga gaacgaagct cttcaagccg ttccgcatcc agacgccgag ggggatgtca
     1921 aaaccgattt ttttcttgtt caggatcgac tccgggaggc ggccggccaa agctttcttg
     1981 aggatgtcct tggaccggaa gccccggagc ttgagggagc tgggaatcgc ggcggcgaac
     2041 tcgacgaacg tgtaatcgag gaacggcgcc cggatctcga gcgaggcggc catgctcatc
     2101 cggtcgactt tcggcagaag gtcgtcctgg aggtaaagct tcaagtcgag gtaggcgatc
     2161 cggtcgagcg gatcggccgc ccgggtgttc gcggcgtgga aggcgaccgg ggcgtaagga
     2221 tcctcggcca gggcggcgtt gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnttcgcggc gtggaaggcg accggggcgt aaggatcctc ggccagggcg gcgttgatgg
     2401 ggggagcgaa cagccgccgc cggagctcgg gcggataggc cccccaccag atgaaattcg
     2461 cgatttccgg cgggtattcc gcgccgctca caaacttttt ggccttgaat tcgaagctca
     2521 gccggcgggc cgaggcggga agggcccgga cgaccggccg gatgaggccg cgtctcgcga
     2581 agccggggac cttccggtac cagcgggccg ccttgtaggc cttgtacgtg tcgtacccgg
     2641 cgaacagctc gtcccctccg tccccggcca gggcgacggt gacgtactct cgggccagct
     2701 tggagatcac gtaggtcggg atgatggagg cgtcggccag gggctcgtcc aggaagccgg
     2761 ccagcgtcgg aacgaggccg ctgacttcgg ccggggtgac gacgaaggcg cggtgatcgg
     2821 tcccgaactt gccggccacg atccgggcgg cgtcgagctc gtcgaacgtt ttttcccgga
     2881 agccgatgga gtacgtcttg atccccgccc cggcgacctc gctcatcagg gcggtgacgg
     2941 cgctggaatc gatgccgccg ctgagaaaga cgccgagggg gacgtcggag atgagccggc
     3001 gccggacgga ttcccggagc cggtcgatga tctccgccgt gtattcggcc gccgggcggg
     3061 ggacaatcgc cgtttcggcg tggcggacgt cccagtagga aagctcggtt tccttcccgt
     3121 cctcgaaaac gagcaggcgg ccgggcagga ttttccggat cccgcggaag atcgagcgcg
     3181 gggccggaat gtactcgaaa gtgaagaacc gggccaggga ctcgaggtcg atgtcccggg
     3241 gaatcggggc ctggaggagg gccttgatct cggaggcgaa atagagcctc gatccgatcc
     3301 ggcaataatg gagcggtttg actcccaagc ggtcgcgggc caggacgagg cggcggcttt
     3361 tcgcgtccca gaggacgacg gcgaacatcc cgttgagggc gtggacgaaa tccgttcccc
     3421 gttcctcgta aagatgaacg acgacttcgg tatcggactg cgtggccagg acatggcccc
     3481 gggcgaggag atccttgcgg atctcgggga aattatagat ttccccgttc atcacgaccc
     3541 agaccgtccg gtcctcgttg gccatcggct ggtgaccggt gacgaggtcg atgatgctca
     3601 ggcggcgcat ccccagggcg gcttgtccat cgatatggaa gccctcgtcg tcgggcccgc
     3661 ggtggatcag ggtgcgggtc atccgcttga gaaggtccgg ggcgccgggg tcgagtcccc
     3721 ggccgtcgac gaatccgcag atgccgcaca tggcgttcat tttaaacgga ttcgttccaa
     3781 aagaccacaa tttcttgcgc cggaagcggc aaaagtgtac cctgaggggc ggaatccggg
     3841 atgaagaaaa tcatcatcaa cgccgacgat ttcggcctgt gcgcgccggt caacgaagga
     3901 atcattctgg cccatcgccg ggggatcctg acgagtgcca ccctgatgac caacacgccg
     3961 ggattcgagc aggcggtcgc cctggcccgg gagaatcccg ccctgggcgt cggcgtgcac
     4021 ctgaacatcg tccgcggccg gccgctttcc ccgccggagg aaatcccgac gctcgtcggg
     4081 cccgacggcc gcttcccggc cggcgcgggg cggattctcc tgaagctggc ggccggccgg
     4141 atccgtcccg ccgagatcga gcgggagctc cgggcccaga tcgagaaagc ccgggcgacg
     4201 gggcttcccc tgacccatct cgacagcgaa aagcacatgc acgccgtccc cgccgttttc
     4261 aagatcgtcc tcaggctggg gcgggaatac ggcttccgca aagtccggtt catccgggaa
     4321 tacggcctct cccgccatcc cgctcaatcc ctgaaagcgg cgtttctttc cctctgctgc
     4381 gcccggatga agcgccgggt ccgggccgcc ggattcgtga tcaccgattc gttttatggt
     4441 atatgtaata gcggccggat gagcgcggcc cggctccgcc ggatttacat ataccataaa
     4501 acgaatcggt gatcacgaat ccggcggccc ggacccggc
//
LOCUS       DAOOXH010000139         4473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_22784_length_4473_cov_8.099819, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000139 DAOOXH010000000
VERSION     DAOOXH010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22784_length_4473_cov_8.099819"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4395)
                     /locus_tag="PKV64_11065"
     CDS             complement(<1..4395)
                     /locus_tag="PKV64_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPN17654.1"
                     /translation="MRKNPLLVGFVILLALAIPASSQDRLRCQNFSLEEGLSQSSSFC
                     IAQDRRGFLWIGTETGLNRYDGYNFKVYLEDRENPRAISNNYVWCLLEAQDGTLWVGT
                     DNGLSRYVPETDDFVNYRPDPGNADSLSNNRIFALAQDHSGFIWIATDEGLNRLDPAT
                     GIFTRYLAGPGGKSGLSHNSIHALLVDRSGVLWIGTDGGGLNRWNEKTGTFDSFRHKE
                     SDPKSLGDDGILSLAEDEAGNLWVGTRNGLDKTGPARDGFLHYRHDGRDPFSLSGATI
                     NCLFLDSSGTLWVGTNEAGIDRYIPARDSFWNYRHDDSNPTSLSGDRILSIIEDASGN
                     LWIGTYGAGLSKYNKNISKFRLYEKGLFTLPGLNINGFRKIIKQGEDIIWAASDGAGL
                     SRLNRATGEIRRFINHPADPASISHNRVYMIHLDDDGYLWAATIEGLNRLDTRTGKFT
                     RYLKKEGDPETLSTNFVRTIVEDKDGFLWLGTDGGGLNRFDKKTGKIKRYSAEEGMPN
                     RLSYARIYALAVDRDGIIWVGTYGGGLNRLDPATGEIKIYSVAEGRADGIKDDYILSL
                     LEDSRGWFWLGTGTGLARFDRDTGAVTVFRMKDGLPDDVIYAMEEDEQGNIWISTNRG
                     LSRFDPVNRKFKNFDIYDGLQSYEFNTYCSHRAADGELFFGGIAGFNSFYPKLITDNP
                     HMPQVALTGFRLFGRDVPINTPIDNRTILDRSITEAAEIRLAYFQNQIDLEFAALDFQ
                     APSKNLYSYRMEGLDTEWTLPSDRRFVSYAGIPPGEYTFRVKAANNDGLWNEEGVALK
                     IEVIPPFWKTRWFQALAILAVLGLALGGVQYRTASMRRRNIHLEKKIKERTADLEQEV
                     AERKRLADEAKRKGAQFALLYDVGQRLSGRLELKELLETIVQSVWEAFDYYGVMLLLH
                     ENGDQVLTLKAISGGYVGAFPLNMKINFGRGMIGMAAASGKTQISGDVEKNPYYFRKA
                     EEKTKSELSVPLIKDDKVIGVLDLQSMSDNAFDESDAAAMETLATQIAAAIENARLYA
                     RAQKEIEDRTRAEEELDRRKKYLETVLFNTPNAIVTTTADEIITEWNPGAEVIFNFRR
                     DEAVGRFIDDLVTTAEERAQAQALTRAAIAQNGLPPTEVVRHRKGGTPIHVIVAGSPI
                     LMGDTLQGMVFVYTDITNRKKAELALQREAAKLSAMISGMEEGVILTDKDDLILEANE
                     YFLKLLKKDRAEMLGRPLWDIDPQLSTGELRTAIADFKSKPFAIPLIQETPFKHLEVI
                     FRLQPIYLNDSYEGVIINLIDVSELIQARKQAQEANRAKSEFLANMSHEIRTPMNGIF
                     GMTELALETDLTAEQRDFMEAVKISAESLMNIINDILDFSKIEAKKIEFETINFNLRD
                     TIHSMVSSVALMAEKKGLELAYHIPGDIPDRVMGDPGRLRQILTNLLSNSIKFTREGE
                     VVVTVQAQSIAEDRMWLHVVVKDSGIGIP"
BASE COUNT          760 a         1276 c         1427 g         1010 t
ORIGIN      
        1 cgggatcccg atgccgctgt ccttgacgac gacgtgcagc cacatccggt cctcggcgat
       61 ggattgggcc tggaccgtga ccacgacttc cccctcccgg gtgaacttga tcgagttgct
      121 cagaaggttc gtcaggatct ggcggaggcg gccgggatcg cccatcaccc ggtccgggat
      181 gtcgcccggg atatgataag ccagctccag gcccttcttt tcggccatca gggccacgct
      241 cgagaccatg ctgtggatgg tgtcgcggag attgaaattg atcgtctcga actcgatttt
      301 tttggcctcg atcttggaga aatccaagat gtcgttgatg atgttcatca gggattcggc
      361 cgagatcttg accgcttcca tgaagtccct ctgttcggct gtcaggtcgg tttccaaggc
      421 cagttcggtc atcccgaaaa tcccgttcat cggcgtccgg atttcgtggc tcatgttggc
      481 caggaattcg ctcttggccc ggttggcttc ctgggcttgt ttccgggctt ggatcagctc
      541 ggagacgtcg atcaggttga tgatcacgcc ctcgtagctg tcgttgaggt agatgggctg
      601 gaggcgaaaa attacttcca ggtgcttgaa cggcgtttcc tgaatgaggg ggatggcgaa
      661 cggcttgctc ttgaaatcgg cgatggccgt ccgcagctcg cccgtcgaca gctgggggtc
      721 gatgtcccag agcggccggc cgagcatctc ggcgcgatcc ttcttgagca gcttcaggaa
      781 atactcgttc gcctccagga tgagatcgtc cttgtcggtc aggatgacgc cttcctccat
      841 cccggagatc atggccgaga gtttggccgc ttcccgctgg agggccagct cggccttctt
      901 gcggttggtg atgtcggtgt aaacgaagac cattccttgg agggtatcgc ccatgaggat
      961 gggcgaaccg gcgacgatga cgtggatcgg ggtgccgccc ttacggtggc ggacgacttc
     1021 ggtcggcgga agcccgtttt gggcgatcgc ggcccgggtg agggcttgcg cctgggcccg
     1081 ttcttcggcc gtggtcacca agtcgtcgat gaaccggccc acggcctcgt cccggcggaa
     1141 gttgaaaatg acctcggccc cgggattcca ctcggtgatg atctcgtcgg ccgtcgtggt
     1201 gacgatggcg ttgggcgtgt tgaacaggac ggtttccagg tatttcttcc gccggtccag
     1261 ctcttcttcg gcccttgtgc ggtcctcgat ctccttttgg gcccgggcgt acagccgggc
     1321 gttctcgatg gctgcggcga tctgggtggc cagcgtctcc atggccgcgg catcggattc
     1381 gtcgaaggcg ttgtcagaca tgctctggag gtcgaggact ccgatgacct tgtcgtcttt
     1441 gatcaggggg accgagagct cggatttcgt tttctcctcg gccttgcgga agtaataggg
     1501 gtttttctcg acgtcgccgc tgatctgggt tttcccggaa gcggcggcca tgccgatcat
     1561 cccccggccg aaattgatct tcatgttgag cgggaaggcg ccgacatagc cgccggaaat
     1621 ggctttcagg gtcaggacct ggtccccgtt ttcgtggagc aggagcatca cgccgtagta
     1681 gtcgaacgct tcccaaaccg attggacgat cgtctccaga agctccttca gctccaggcg
     1741 gccgctcagc cgctggccga cgtcgtaaag cagggcgaac tgggccccct tgcgcttggc
     1801 ctcatcggct aggcgcttgc gttcggccac ttcctgctcg aggtcggccg tccgctcctt
     1861 gatttttttc tcgagatgga tgttgcggcg gcgcatgctg gctgtccggt attgaactcc
     1921 gcccagggcc agcccgagga ccgccagaat cgccagggct tggaaccagc gcgtcttcca
     1981 gaacgggggg atcacctcga tttttaaagc gacgccctcc tcgttccaga ggccgtcgtt
     2041 gttggccgcc ttgacgcgga acgtgtattc gccgggagga atgccggcgt aggagacgaa
     2101 ccggcggtcg gaaggaagcg tccattccgt gtccaggcct tccatgcggt aggaatacaa
     2161 gttcttgctg ggagcttgaa aatcgagggc ggcgaattcc aggtcgatct ggttctggaa
     2221 ataggccagg cggatctccg cggcttcggt gatcgagcgg tcgaggatcg tgcggttgtc
     2281 gatcggcgtg ttgatgggga cgtcgcgtcc gaagaggcgg aagccggtga gggcgacctg
     2341 cggcatgtgc ggattgtcgg tgatcagctt gggatagaac gagttgaagc ccgcgatgcc
     2401 gccgaagaaa agctcgccgt cggccgcccg gtgggagcaa tacgtgttga attcgtagct
     2461 ttggaggccg tcgtagatgt cgaagttctt gaatttccgg ttcactggat cgaatcgcga
     2521 caggccccgg ttggtcgaaa tccagatgtt gccctgctcg tcctcctcca tggcgtagat
     2581 gacgtcgtcg ggcaggccgt ccttcattct aaaaacggtg accgctcccg tgtcccggtc
     2641 gaaccgggcc aggccggttc ccgttcccag ccagaaccag ccgcggctgt cctccaggag
     2701 gcttaaaata taatcgtcct tgatcccgtc cgcccgtccc tcggccacgc tgtagatctt
     2761 gatctcgcct gtcgccgggt cgaggcggtt gagtccgccg ccgtaggttc ccacccagat
     2821 gattccgtcc cggtcgacgg cgagagcgta gatccgggcg tagctcaacc ggttcggcat
     2881 cccttcttcg gccgagtacc gcttgatctt gcccgttttc ttgtcgaacc ggttcaagcc
     2941 gccgccgtcg gttccgagcc agagaaaacc gtccttgtcc tcgacgatgg tccggacgaa
     3001 attcgtgctc aaggtctccg ggtcgccttc ttttttcaag tagcgggtga atttccccgt
     3061 ccgggtgtcg agacggttca gcccctcaat ggtcgccgcc caaaggtatc cgtcgtcgtc
     3121 gagatggatc atgtagacgc ggttgtggct gatgctcgcc ggatcggccg gatgattgat
     3181 gaagcggcgg atctctccgg tggccctgtt caaccggctc aagccggcgc cgtcggaggc
     3241 cgcccagatg atgtcttccc cttgcttgat gattttacga aagccgttga tgttcaggcc
     3301 gggcaaggtg aagaggccct tttcataaag gcggaacttg gagatgttct tgttgtattt
     3361 gctgaggccg gccccgtatg tcccgatcca gaggttgccc gaagcgtcct cgatgatcga
     3421 gaggatgcgg tcgccggaca ggctggtcgg gttgctgtca tcgtggcggt aattccagaa
     3481 cgaatcgcgg gcggggatat accggtcgat gccggcctcg ttggttccca cccagagcgt
     3541 tccggaagag tccaggaaga ggcagttgat cgtggcgccg ctcaggctga agggatcgcg
     3601 gccgtcatgg cggtaatgaa ggaagccgtc ccgggcgggt ccggtcttgt ccaggccgtt
     3661 gcgcgtcccc acccagagat tcccggcctc gtcctcggcc agcgacagga tgccgtcgtc
     3721 cccgaggctt ttcggatcgg actccttgtg gcggaaggag tcgaatgttc cggttttttc
     3781 gttccaccgg ttcaggccgc ccccgtcggt tccaatccaa aggacgccgc tccggtccac
     3841 gagaagggca tggatggaat tatggctgag cccggatttc ccgcccgggc cggccaagta
     3901 gcgggtgaag atcccggtcg cgggatcaag gcggttcagg ccttcgtcgg tggcgatcca
     3961 gatgaaaccg gagtggtctt gggcgagggc gaaaatccgg ttgttgctca ggctgtcggc
     4021 gttcccggga tccggccggt aattgacaaa atcgtccgtc tccgggacgt agcggctcaa
     4081 gccgttgtcg gtcccgaccc aaagggttcc atcctgggct tccagcaggc accaaacgta
     4141 attattgctg atggccctcg gattttcccg gtcttccagg tagaccttaa aattgtagcc
     4201 gtcgtaccgg ttaaggcccg tttcggttcc gatccagaga aatcccctgc ggtcttgggc
     4261 gatgcagaag ctggaacttt gcgagagccc ctcctccaag gaaaagttct ggcagcggag
     4321 acgatcctga gaggatgcgg gaatcgccaa ggccagaaga ataacgaatc cgacgaggag
     4381 cgggtttttc cgcatctgag acctccagcg ggtgcttctt aattccgtta agttaatcca
     4441 aacttgaaat tttttcaatc tttccggaat aaa
//
LOCUS       DAOOXH010000140         4310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_24022_length_4310_cov_5.324089, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000140 DAOOXH010000000
VERSION     DAOOXH010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24022_length_4310_cov_5.324089"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PKV64_11070"
     CDS             complement(<1..965)
                     /locus_tag="PKV64_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPN17655.1"
                     /translation="MIFIYIGLPLAVAAVLPLAGKASRRVLPDILSNAVLAGLLAYAV
                     TGAKALLVHGPIVQQVRWFGEAVNIRIALDGFSLFMLMAIQLVSLCVGLYSINYMEHY
                     GAKANYYALLLVMVAGMNGLVLSQDLFSVYLFLEVAAVASYALVAYGLEAEELEASFK
                     YLMLSVVASAAVLVAITVLYGMTGALGFDAVAEGLRLLEAKAVVGVAAALFLMGFGLK
                     AALIPFHAWLPDAHPSAPAPISAILSGLLIKVSGVYAITRIFFHVIGLTPALSQVLMW
                     LAAVSVVVAAFLALGQKDMKRMLAYSSISQVGYVVLGLGLGTPLGV"
     gene            complement(982..2853)
                     /locus_tag="PKV64_11075"
     CDS             complement(982..2853)
                     /locus_tag="PKV64_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPN17656.1"
                     /translation="MTPSTLLWTILLPALTGIAFFLIPRTVKYVREVLAVVSAGVSLY
                     LGFALFAVKNLAWQAPWMGSGIPMELRLYQFPAFILLGLSGFLFLIALYSAAKMKDHP
                     RVREYYAYVFLSAALAFGAVLADNFVPLVFFWEGLLVTLYALISLGGKEAHRTATKAF
                     LINGFCDFAMILGIGIVWSLAGTMTISQVSLKPEGPAALAFVLMMIGAIGKAGAMPFH
                     TWIPDAAIDAPVTFMAYMPAAFEKLLGIYLLARICLDIFTLQPGSGLSLLLMIIGAAT
                     IVLAVMMALVQKDLKRLLSYHAVSQVGYMILGIGTAIPIGIAGGIFHMINHAMYKSGL
                     FLSAGSVEHRAGTTDLRKLGGLAREMPITAAGFTVCALAISGVWPLNGFVSKEMVFHG
                     AVESGSIIFAIAAWAGAVFTFASFLKAGHSVFFGKRSEERAPGRVKESPVTMLIPILI
                     LALLCITFGLFNKLPLTQFIQPVVAAHAEAGAPIDYTAHALDLFNVIAGISVACLILA
                     FGLHVYGFKRGGKKAWLASEPIHHAPFFKQMYDGAEKRFFDLYEQGIKFLRGLSWILF
                     KGIDRPIDAVYEKGVTAVGTAFTGLLRKAHNGHYANYLAWGLGGLVVLIALINVILK"
     gene            complement(2850..4304)
                     /locus_tag="PKV64_11080"
     CDS             complement(2850..4304)
                     /locus_tag="PKV64_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HPN17657.1"
                     /translation="MSILSGPPMSQETITLLTVLVPIIGSMTIPLAGLISRAARSVWA
                     AALGAATSVLAMSLLPRALAGGETVVRKNLVLGLDFILLIDPLSIFMACVSSFVGFLI
                     IVYSIGYIRDEENQNEYYLLVVLFIGSMMGLVFSGNLIFLYLFWEIIAICCWRLIGFY
                     RLKEHVKKADKAFLITFFGAVVMLLGFILIYGQTGSFDLTVMRGLPVSGTAVLLILFG
                     MFSKSATVPLHTWLPDAGVAPTTVTALLHAAVLVKIGVYAFARLFVYGFRVPEGWADA
                     LPWIAVVSSLVAAAAATVENDFKRILAYSTVSQIGYIFLGYAVLTDAGVTGSTLFILM
                     HGLAKAGLFLCAGIVIHATHKRDIREMGGLFKSMPLTALSFLFCAFSVIGIPPFGGFF
                     SKFLVIMGTVKAGRVGVAAVALFTAVLTMFYLLRVFSQVFLGEVKAPAHEGTRSMVAV
                     VSILAVLSLVAGLLIAYPSVLAETATAHLMGWLK"
BASE COUNT          995 a         1251 c         1446 g          618 t
ORIGIN      
        1 acgccgagag gcgttcccag cccgaggccg aggacgacgt atccgacctg gctgatggag
       61 gaataagcca gcatccgctt catgtccttt tggccgagag ccaggaaggc ggcgacgacg
      121 accgaaacgg ccgccagcca catcagcacc tgggacaggg cgggggtcag gccgatgacg
      181 tgaaagaaga tccgggtgat ggcgtaaacg cccgagactt tgatcagcag gcccgaaaga
      241 atggccgaaa tgggggccgg ggccgaaggg tgggcgtcgg gaagccaggc atggaagggg
      301 atcagggccg ctttcagccc gaagcccatg agaaacaggg cggcggcgac gccgacgacg
      361 gctttggcct ccaaaagccg gagaccctcg gccacggcgt cgaagcccag ggctccggtc
      421 atgccgtaga ggacggtgat cgccaccaag acggccgccg aggccacgac cgagagcatg
      481 aggtatttga acgaggcttc gagctcctcg gcttccaggc cgtaggcgac cagggcgtag
      541 gaggccacgg ccgcgacttc gaggaacaga tagacgctga aaagatcctg ggaaagaacg
      601 aggccgttca ttcccgcgac catcaccagc agaagggcgt agtaattggc cttggcgccg
      661 taatgctcca tgtaattgat ggaatagagg ccgacgcaca ggctgaccag ttggatcgcc
      721 atcagcatga acaggctgaa gccgtccagg gcgatccgga tgttgacggc ctccccaaac
      781 caccgcacct gctggacgat cggcccgtgg accaggagcg ccttggctcc ggtcacggca
      841 taggccagaa ggcccgccag gacggcgttg gagaggatgt cgggcagaac ccgccgggac
      901 gctttgccgg cgagaggcag gacggcggcc accgccaggg gcaaaccgat gtagatgaaa
      961 atcaaaagtc ctcctctctc gttacttcaa aatgacgttg atcagggcga tcaggacgac
     1021 cagcccgccc aggccccagg ccaaatagtt ggcatagtga ccgttgtggg cctttcgcag
     1081 cagaccggtg aacgctgttc cgaccgcggt gacgcccttt tcgtagacgg cgtcgatcgg
     1141 ccggtcgatg cccttgaaca agatccagga caagccgcgc aggaatttga tcccctgttc
     1201 gtaaagatcg aagaaccgct tctcagcgcc gtcgtacatc tgcttgaaga acggagcgtg
     1261 atgaatgggc tcggaagcca gccaggcctt tttcccgccg cgcttgaacc cgtagacgtg
     1321 aagaccgaag gccaggatca ggcaggctac cgagatgccg gcgatgacgt tgaaaagatc
     1381 caaggcgtgg gcggtgtaat cgatcggcgc gcccgcctcg gcgtgggcgg cgacgacggg
     1441 ctggatgaat tgcgtcaggg gcagcttgtt gaacaagccg aaggtgatgc acaaaagggc
     1501 gagaatcagg atcgggatca gcatggtgac cgggctttcc ttgacccggc cgggggcccg
     1561 ctcctcggac cgcttcccga agaagaccga gtgtccggcc ttgaggaacg aggcgaaagt
     1621 gaagacggcc ccggcccaag cggcgatcgc gaagatgatc gagccggact ccacggcgcc
     1681 gtggaagacc atttccttcg agacgaagcc gttgagcggc cagacgcccg aaatcgccag
     1741 ggcgcagacc gtgaacccgg cggccgtgat cggcatttcc cgggccaggc cgcccagctt
     1801 ccgaagatcc gtcgtcccgg cccggtgttc gaccgagccg gcgctgagga agagcccgct
     1861 cttgtacatg gcgtggttga tcatgtgaaa aatcccgccg gcgatgccga tcgggatggc
     1921 cgtgccgatg cccaggatca tgtatccgac ctggctgacg gcgtggtagg acaacagccg
     1981 cttcaaatcc ttctggacca gggccatcat gacggccagg acgatcgtcg cggctccgat
     2041 gatcatcaga aggagagaaa ggcccgagcc gggctgaagc gtgaagatat ccaggcagat
     2101 ccgggcgagc aggtaaatgc ccagaagctt ctcaaaggcg gccggcatgt aggccatgaa
     2161 agtcaccggc gcgtcgatcg cggcgtccgg gatccaggtg tggaagggca tcgcgcccgc
     2221 cttgccgatc gccccgatca tcatcaggac gaaggcgagg gcggccggtc cctcgggctt
     2281 gagcgagacc tgggaaatgg tcatcgtccc ggcgagcgac caaacgatcc cgatgccgag
     2341 gatcatggcg aaatcgcaga aaccgttgat caggaacgcc ttggtcgccg ttcgatgggc
     2401 ttccttgccc cccagcgaga tcagggcgta cagggtgacc aggagccctt cccagaagaa
     2461 aaccaggggg acgaagttgt cggccaggac cgccccaaag gccagggcgg ccgagaggaa
     2521 gacgtacgcg tagtactcgc ggacgcgcgg atggtccttc atcttggccg ccgagtacag
     2581 ggcgatcagg aaaaggaacc cggagaggcc gaggaggata aaggccggga actggtaaag
     2641 ccggagctcc atcgggatcc cggagcccat ccacggggct tgccaggcca gattcttgac
     2701 ggcgaacagg gcgaacccga gatagaggga aacgccggcg gaaacgaccg ccagaacctc
     2761 gcggacgtat ttgaccgtcc gggggatcaa gaaaaaggcg atgcccgtaa gggccggcag
     2821 aagaatggtc cagagaagag ttgaaggcgt catttgagcc accccatgag atgagccgtg
     2881 gccgtttcgg ccaggaccga aggataggcg atcagcaggc cggcgaccag ggagaggacg
     2941 gccaggatcg agacgacggc gaccatggac cgcgtccctt catgggccgg agctttgact
     3001 tctcccagaa aaacctggct gaaaacgcgc aggaggtaaa acatggtcaa aacagcggtg
     3061 aacagggcca cggcggcgac gccgactcgg cccgccttga ccgtccccat gatcaccagg
     3121 aatttagaaa agaagccgcc gaagggcggg atgccgatga ccgagaaggc gcagaagagg
     3181 aaggagaggg ccgtcagggg catcgatttg aacagcccgc ccatctcgcg gatgtcccgc
     3241 ttgtgggtgg cgtggatgac gatcccggcg cagaggaaca gccccgcctt ggccaagccg
     3301 tgcatcagga tgaacagcgt cgatcccgtg accccggcgt ccgtcaggac ggcgtagccg
     3361 agaaagatgt agccgatctg gctgaccgtc gaataggcca gaatccgctt gaaatcgttt
     3421 tcgacggtcg ccgcggcggc cgcgaccagg ctggaaacga ccgcgatcca ggggagcgcg
     3481 tcagcccagc cctccggaac ccggaagccg tagacgaaca accgggcgaa ggcgtagacg
     3541 ccgattttga cgaggacggc cgcgtgcagc agagccgtga ccgtcgtcgg ggcgacgccg
     3601 gcgtccggaa gccaggtgtg caggggaacc gtggccgatt tcgagaacat cccgaaaagg
     3661 atcaaaagaa cggcggttcc ggaaacgggc agccccctca tcaccgtgag gtcgaagctg
     3721 ccggtttgac cgtagatcag gatgaacccg agcagcatga ccaccgcgcc gaagaaggtg
     3781 atcaggaagg ccttgtcggc cttcttcacg tgctccttca ggcggtagaa gccgatgagt
     3841 cgccagcagc agatggcgat gatctcccag aacaagtaga ggaagatcag gtttcccgaa
     3901 aacaccagcc ccatcatgct gccgatgaac aggacgacca ggaggtaata ctcgttctgg
     3961 ttctcctcgt cccggatgta cccgatcgaa tacacgatga tcaggaatcc gacaaacgaa
     4021 gagacgcagg ccatgaagat cgacaagggg tcgatcaaca ggatgaagtc cagccccaga
     4081 acgagattct tccggacgac cgtttctcct ccggccaggg cccggggcaa cagggacatc
     4141 gccaggacgg aggtcgcggc gccgagcgcc gcggcccaaa ccgaccgggc ggcccgggaa
     4201 atcaggcccg ccagggggat cgtcatcgag ccgatgatag gaacgaggac cgtgagtagg
     4261 gtaatggttt cttggctcat gggtggaccg gaaagtatac tcatcggatc
//
LOCUS       DAOOXH010000141         4293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_24161_length_4293_cov_6.256961, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000141 DAOOXH010000000
VERSION     DAOOXH010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24161_length_4293_cov_6.256961"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PKV64_11085"
     CDS             complement(<1..766)
                     /locus_tag="PKV64_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPN17658.1"
                     /translation="MNDEKPLGDRIRETLIGKPKSIKDPSLFHKLALIPILAWIGLGA
                     DGLSSSSYGPEEAFKTLGTHTYLALFLAVATALTVFIISKAYARIIEYFPSGGGGYVV
                     ATQTLGPKAGVLSGAALLVDYVLTITVSIVSCGAALFSFLPVSWHDYKIPFDLALIVI
                     LVILNLRGVKESIQLLAPIFIIFVLTHLLLIGYGILSHAPRIGPVSAKIGGDFQAGLA
                     TLGAGGMILLFLKAFSMGGGTYTGIEAVSNGLGILRE"
     gene            1029..2870
                     /locus_tag="PKV64_11090"
     CDS             1029..2870
                     /locus_tag="PKV64_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015848762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HPN17659.1"
                     /translation="MKGWTGNILRVNLSSKTYKKETFSEEFAHKWVGGRGFAVKTLYD
                     ELKPGIDPLGPDNKFIVALGPISGIPAPNTGKAVVAAKSPLTGFYGDGNLGTRAVEQL
                     RKAGYDMLIVEGKADRPTMLYIEDDKVEFLPADEIWGKGTFACNDWIYSKYGKGVGVL
                     NIGQGGENLARFAVVRSLEGRAGGRPGIGAVMGSKLLKAIVVKGTRPIPQADPAGMKA
                     AGSADLRQVGVLDKQSGWSIQATTGVLPFCNEVAGLPVRNFRKTHHPLAWQVDGERCN
                     NARVATYGCPTCTMRCGVTVLDKEGHESELDYENVALLGPNLEIFDIPQMASLNYLCD
                     EFGLDTMSAGCTLSFYADAIDQGAVDGDFKFGDAERAKELLRLAAHREGKVGHLLADG
                     TRRMARAFGRGSEAYAMNVKGLEVAAYNCKFIPGQALSYGVAPIGAHHREAWIITFEI
                     KHTTRESYGPEKAAKVIELQRIRGGLFEFIVACRFPWIENGWPLDHYPKYFNLATGLN
                     WGLDDMWQVGDRIYSLIKLHYLREFPGASREDDYPPRVWFDPANADTEGPIAGKHLEI
                     DKYDGLLQHYYDQRGYDRRGIPTMATLRSLGLEDEAADARKYADLTE"
     gene            2876..3103
                     /locus_tag="PKV64_11095"
     CDS             2876..3103
                     /locus_tag="PKV64_11095"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HPN17660.1"
                     /translation="MKVRVKVIGPLIYKTGFSERDLELDAETTVRDLVEQIGIPPERP
                     RIVTRNGKAVLPEDRLADGDTIAVSPIYSGG"
     gene            complement(3343..3624)
                     /locus_tag="PKV64_11100"
     CDS             complement(3343..3624)
                     /locus_tag="PKV64_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HPN17661.1"
                     /translation="MMKVNEYFGGKVKSIGFTTEGGPATIGVMAPGDYEFGTSTREIM
                     TVVSGVLNVKLPGSEEWKAFKAGESFTVEKDRKFQLKVPGDAAYLCLYR"
     gene            complement(3621..4241)
                     /gene="thiF"
                     /locus_tag="PKV64_11105"
     CDS             complement(3621..4241)
                     /gene="thiF"
                     /locus_tag="PKV64_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HPN17662.1"
                     /translation="MAGTEPFSQPLRPEVAAVLKTSVVGVAGAGGLGSNVAFHLARAG
                     VGKLIIADFDRVETSNLNRQQYFIDQVGRFKVEALAANLRRIPSATVVVFRRTTVTAR
                     NAARLFGEADVLIEAFDRAGSKQMLIEAWMKAFPERPVIAASGLAGYGANNRIRERRF
                     GRLTLIGDGAGESPAAVSPMAPRVGIVAAMQANRAIECLMKKRRKT"
BASE COUNT          814 a         1408 c         1304 g          767 t
ORIGIN      
        1 gctcccgaag gatgcccagg ccgttggaga ccgcctcgat cccggtgtaa gtccccccgc
       61 ccatggaaaa ggccttaagg aagagtaaga tcatgccgcc ggctccaaga gtcgccagcc
      121 ccgcctggaa atcgccgccg attttggccg aaaccggccc gatccgggga gcatggctca
      181 agattccgta gccgatcagc aggaggtggg tcaggacgaa gataatgaaa atcggggcga
      241 gaagctggat cgactctttc actccgcgga ggttgaggat cacgaggatg acaatcaagg
      301 ccaggtcgaa cggaatcttg tagtcatgcc aggagacggg cagaaagctg aacaacgccg
      361 ccccgcagga gacgatcgag acggtgatgg tcaggacata gtccaccagc agggcggccc
      421 cggagaggac cccggccttg ggacccagcg tttgagtggc cacgacgtat ccgccgccgc
      481 cggagggaaa atattcgatg atgcgggcgt acgccttgga aatgatgaag accgtcaggg
      541 ccgtggccac ggccagaaaa agcgccaggt acgtatgcgt ccccagcgtc ttgaaggctt
      601 cctcgggacc atacgacgaa gaggagaggc cgtcggcccc gaggccgatc caagccagga
      661 tgggaatcag ggccagcttg tgaaaaaggg acggatcctt gatgcttttc ggcttgccga
      721 tcaacgtttc cctgatccgg tctcccaggg gcttttcgtc gttcatctcc ctctccggcc
      781 gacttccccg gatcgccttt caatcccccc gcctcccccg gccgaggatg aattcctgag
      841 tcgaggaggt cgtggggggt ggtttccgcg tgatccggcg gcttcgagcc tggattatcc
      901 ctccggcggg cgaaaatgtc aaaccggccg gcccccggcg ccggccgcgc cttgactctt
      961 cggtcccggc atggtatcaa gtatttctcc ctcattacgc atatttatct caattcctgg
     1021 aggctttcat gaaaggatgg accggcaata tcttacgagt caatctctct tccaagacgt
     1081 ataaaaaaga gacgttttcc gaggaattcg cccataaatg ggtcggcggc cgcggtttcg
     1141 cggttaaaac cctgtacgac gagctcaagc ccgggatcga tcccctcgga ccggacaaca
     1201 agttcatcgt cgccctcggc cccatttccg gaatcccggc tcccaacacc ggcaaggccg
     1261 tcgtcgcggc caaatcgccg ctgaccggat tctacggaga cggcaacctg ggaacgcggg
     1321 cggtcgaaca gctccggaaa gcgggctacg acatgttgat cgtcgagggc aaggccgacc
     1381 ggccgacgat gctctacatc gaagacgaca aggtcgaatt ccttccggcc gacgaaatct
     1441 ggggaaaagg gaccttcgcc tgcaacgact ggatctattc gaaatacggt aagggcgtcg
     1501 gcgtcctgaa catcggccaa ggcggggaga acctcgcccg tttcgccgtc gtccggagcc
     1561 tggaaggccg ggccggcggc cggccgggca tcggggcggt catgggctcc aagctcctca
     1621 aggccatcgt cgtcaaaggc actcgtccca tcccccaggc cgatcccgcc ggaatgaagg
     1681 ccgccggctc ggccgacctt cgtcaggtcg gggttctcga caagcagtcg ggctggtcga
     1741 tccaagccac caccggcgtc cttcccttct gcaacgaggt cgccggcctg cccgtccgca
     1801 acttccggaa aacccatcac cccttggcct ggcaggtcga cggagagcgc tgcaacaacg
     1861 cccgggtggc gacctacggc tgcccgacct gcacgatgcg ctgcggcgtc accgtcctgg
     1921 acaaggaagg ccacgaatcc gagctcgatt atgaaaacgt cgctctcctg ggccccaacc
     1981 tcgaaatttt cgatatcccc cagatggcct cgctcaacta tctttgcgac gaattcggcc
     2041 tcgacacgat gtcggccggc tgcacccttt ccttctatgc cgacgccatc gaccaagggg
     2101 cggtggacgg cgatttcaag ttcggcgacg ccgagcgggc caaggagctc ctccgcctgg
     2161 ccgcccatcg ggaaggcaag gtcggccatc tcctggcgga cggaacccgg cgcatggccc
     2221 gggctttcgg ccgcggctcc gaggcctacg ccatgaacgt caagggcttg gaagtcgcgg
     2281 cttacaactg caagttcatc cccggccagg ccttgtccta cggtgtcgcg cccatcggag
     2341 cccatcaccg ggaagcctgg atcatcacct tcgagatcaa gcacacgacc cgggaatcct
     2401 acggcccgga aaaggcggcc aaggtcatcg agctccagcg catccggggc ggcttgttcg
     2461 aattcatcgt cgcctgccgc ttcccctgga ttgaaaacgg ctggccgctc gatcactatc
     2521 cgaagtactt caacctggcc accggcctga actggggctt ggatgatatg tggcaagtcg
     2581 gcgaccggat ctattcgctc atcaagctcc actatctccg cgaattcccc ggcgccagcc
     2641 gggaggatga ttatccgccg cgggtctggt tcgatccggc caacgccgac accgagggcc
     2701 cgatcgccgg aaaacacctg gagatcgaca aatacgacgg cctgctccag cactattacg
     2761 accagcgcgg ttacgaccgg cgcggcatcc cgaccatggc gacccttcgt tccctgggcc
     2821 tggaggacga ggccgccgac gcccggaaat acgccgacct gacggagtga cgctcatgaa
     2881 agtccgcgtc aaggtcatcg gacccttgat ctataagacc ggtttcagcg aaagggacct
     2941 cgagctggac gcggaaacga ccgtccggga tctcgtcgaa cagatcggca tccccccgga
     3001 gcgcccccgg atcgtgaccc ggaacggaaa ggccgtgctt ccggaggacc gtctcgcgga
     3061 cggcgacacg atcgccgttt cgcccatcta ttccggcggc tgaggtccgc gcctcggaca
     3121 gaccggtgtc tttgcggttg aaacgccttt cgccgggccg gactcccggc cgagaccgcg
     3181 gaagtatttc cagcggcctg gattcccgga ttcggcatcg gccggcgacc cgttttattt
     3241 tcttccgccg acgtttttcc gaccgaaaat tcgtccagcc ggacggtttc ccgttcttcc
     3301 gcgggattcc gatccggccg gacggaggcg gcgcctcggc tctcagcgat agaggcagag
     3361 ataagcggcg tcgccgggga ccttcaactg gaattttcgg tctttctcga cggtgaacga
     3421 ttcaccggcc ttgaacgcct tccattcctc gcttcccgga agcttgacgt tgaggacgcc
     3481 gctgacgacc gtcatgatct cccgggtgga cgtgccgaac tcgtagtcgc ccggggccat
     3541 caccccgatc gtggccgggc cgccttcggt cgtaaaaccg atcgatttga cctttccgcc
     3601 gaaatattca ttgactttca tcatgttttt ctcctttttt tcattagaca ttcgatggcc
     3661 cggttggcct gcatggccgc gacgattccg acccgcgggg ccatcggcga aaccgccgcc
     3721 gggctttccc ccgccccgtc gccgatgagc gtaagccgtc cgaaccgccg ctccctgatc
     3781 cggttgttgg ccccgtatcc ggccagtccc gaggcggcga tgaccggccg ttccgggaag
     3841 gccttcatcc aggcctcgat cagcatctgc ttgctcccgg cccggtcgaa cgcctcaatc
     3901 aagacgtcgg cctcaccgaa gaggcgggcg gcgtttcggg ccgtgaccgt cgtccgccgg
     3961 aaaacgacga ccgtggccga gggaatccgc cggaggttgg ccgccagggc ctcgaccttg
     4021 aagcggccga cctggtcgat gaaatactgc tggcggttga ggttggacgt ttccacccgg
     4081 tcgaaatcgg cgatgatgag cttccccacg ccggcccggg ccaggtggaa agcgacattg
     4141 gagccaagcc ctccggcccc ggcgacgccg acgacgcttg tcttgaggac ggcggcgacc
     4201 tccggacgga gcggctgcga aaacggttct gtgcccgcca tgatccggag gtcgccgccg
     4261 tcctcaagcc aagcgtcgtc ggcgtcgccg ggg
//
LOCUS       DAOOXH010000142         4061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_26125_length_4061_cov_7.491263, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000142 DAOOXH010000000
VERSION     DAOOXH010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26125_length_4061_cov_7.491263"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="PKV64_11110"
     CDS             <1..927
                     /locus_tag="PKV64_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17663.1"
                     /translation="PERLPSVINRRYGDPITTDTITSSGRFEHYDHESVGRYVAAKLR
                     KDDVVIAIHVVFQKIYAGRVDYWLWSGGSGTWDAWEKTKDGWKDFYVGARWINNADDL
                     RRTIQAHPGQRIWLVTSPSMFRTDHINTALYDFISVENADKLAYRGQDGMSEVYVWND
                     PELTAGGRRACEGEWLAVRRGAVVTGPGMSGFAAVSWDGSAAKTDDSTTKIVRRLAPG
                     SYRASVRYKAAPGQADKTAGRILLVPGKGRPLRTIILSGDARRPDADGWRIADVEFLV
                     PRETAVSVRVQVPAGCVLMIDYLDLRLKEGRS"
     gene            924..1694
                     /locus_tag="PKV64_11115"
     CDS             924..1694
                     /locus_tag="PKV64_11115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPN17664.1"
                     /translation="MKDERAPGSTQKELLDPKRKLAGKYAGLCIGKKGLAALLKYEFV
                     NLFIAPIPGALGLVLRRAFFPWILGKVGRGVVFGRNVVLRHPHKIEIGDNTFIDDGAV
                     LDAKGEANEGIRIGANAMIGRNTILTCKEGSIYLGDHCNVSANCSLLSETEIRLGDYC
                     FLAGECYLVAGGNHPVADTSRPIMFQASSAKGGIRVGEDTWMGAGVIVLDGVTIGKGS
                     VIGAGALIAASLPEYTFAVADRRLRLADRRTLADRNPA"
     gene            complement(1732..2526)
                     /locus_tag="PKV64_11120"
     CDS             complement(1732..2526)
                     /locus_tag="PKV64_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17665.1"
                     /translation="MRRFILPAAGAAFLLIPAALSAQASLPVETLLDRVAARLESFNP
                     KSNWQASIRSTQIELDRKGRPERTTVQTKAVAVIEGRREEDILSVVRTEDGVTRDITE
                     KYLAERRARWEKYRAQETARERGKEPARRRSRLGMRVEDFAGLLPFSPERRTEFSFAA
                     REGAAPDGRPLYFLDVRAKVKDVENWEGTYTIDAATFTPFHARLTPSETPPFVKEIEV
                     EADFQVFEGVHIIPLRTWVRINAGFLFLKRIRAVSEEVYTDIRVTR"
     gene            2730..2873
                     /locus_tag="PKV64_11125"
     CDS             2730..2873
                     /locus_tag="PKV64_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17666.1"
                     /translation="MSTFLIVVLGVLGFLALVIGFLGWLFFHQGGCCVKKTAAKISRK
                     CAV"
     gene            3066..3917
                     /locus_tag="PKV64_11130"
     CDS             3066..3917
                     /locus_tag="PKV64_11130"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HPN17667.1"
                     /translation="MGPIKNEVAVMFSGGIDSLLAAVLLQKEFEKVHLITFDKGYLEF
                     GVRNNLPNVERLKAAFGEDKITHQIIDHKPIIKKIAMSSFFKNRDKYNAETRMCVGCR
                     LSMNTGGLLFALENDLVGFADGSNREQIPSPANLTGTAENYPSIVNGGKRFAQEYGVH
                     FMTPTYEYGNRDERRAKLKELGYDIDYLSMDESKKLSGLVSKGLGKRSQPMCFSGWLI
                     HWKRNLFGKPVEQNEAMTVDYVTEKQNGVVRDVIQDYFADKGIDIDALVKARKADPRG
                     QFTPDFV"
BASE COUNT          756 a         1271 c         1304 g          730 t
ORIGIN      
        1 ccggagcggc tcccgtccgt gatcaaccgc cgttacgggg atccgatcac gaccgacacc
       61 atcacctcct cgggccggtt cgagcattac gaccacgaga gcgtcggccg gtatgtcgcg
      121 gccaagctcc ggaaagacga tgtcgtcatc gccatccatg tcgtttttca gaagatctac
      181 gccggccggg tggattactg gctgtggtcg ggcggatcgg gcacctggga cgcctgggag
      241 aagacgaagg acggttggaa ggatttttac gtcggggccc gctggatcaa caacgcagat
      301 gatcttcgcc ggaccatcca ggcccatccg gggcaacgga tctggctggt gacgtcgccg
      361 tcgatgttcc ggaccgacca catcaacacg gccctctacg acttcatctc cgtcgaaaac
      421 gccgacaagc tcgcataccg cggccaggac ggcatgagcg aggtctacgt ctggaacgac
      481 cccgaattga cggccggcgg ccggcgggcc tgcgaggggg aatggctggc cgtccgccgc
      541 ggggcggtcg tcaccggtcc cggaatgtcg ggattcgcgg ctgtttcctg ggacgggagc
      601 gcggccaaaa ccgacgattc gacgacgaag atcgtccgcc gcctggcccc cggctcctac
      661 cgggcttcgg tccgctacaa ggccgccccc ggccaggcgg ataaaacggc gggacggatc
      721 ttgctggttc cgggaaaggg ccggcccttg cggaccatca tcctttccgg ggacgcccgc
      781 cgtcccgacg cggacgggtg gcggatagct gacgtcgaat tcctcgtccc ccgtgagacg
      841 gccgtctccg ttcgggtcca ggtcccggcc ggatgcgttc tgatgatcga ttacctcgat
      901 cttcggctga aggaagggcg gtcatgaagg acgagcgggc tccggggagc acgcagaaag
      961 agctcctcga tcccaagcgg aagctcgccg gcaaatacgc cggcctgtgc atcgggaaaa
     1021 agggtttggc ggccttgctg aaatacgagt tcgtcaatct cttcatcgcc ccgatccccg
     1081 gcgcgctggg gctggttctc cgacgggcct ttttcccctg gatcctgggg aaggtcggcc
     1141 ggggcgtcgt cttcggccgg aacgtcgtcc tccgccatcc gcacaagatt gaaatcgggg
     1201 acaacacgtt catcgacgac ggggccgtcc tggacgccaa gggcgaggcc aacgagggga
     1261 tccggatcgg ggccaacgcc atgatcggcc gcaacacgat cctgacctgc aaggaaggat
     1321 ccatttatct gggggaccac tgcaacgtct cggccaactg cagcctgtta tcggagacgg
     1381 agatccggct cggcgattat tgttttctgg ccggggagtg ttatctcgtg gccggcggca
     1441 atcatcccgt cgccgatacg tcccgcccga tcatgttcca agcctcgtcg gccaagggcg
     1501 gcatccgggt cggagaagac acctggatgg gcgccggagt catcgtcctg gacggagtga
     1561 cgatcgggaa gggcagcgtc atcggggccg gggctctcat cgccgcctcg ctccccgaat
     1621 acacgttcgc cgtggccgac cggcgtctgc gcctggccga ccggcggacg ctcgcggacc
     1681 ggaatcccgc ctagcggcct tcccccggcc gccgtccccg gatcctcccg ctcagcgggt
     1741 gacgcggatg tccgtgtaca cttcctccga gacggcccgg atgcgcttga ggaagaggaa
     1801 tccggcgttg atccggaccc aggtccggag gggaatgatg tggacgccct cgaagacctg
     1861 gaaatccgcc tcgacctcga tctccttgac gaagggcggc gtctccgacg gcgtgagccg
     1921 ggcgtgaaac ggcgtgaacg ttgcggcgtc gatcgtatag gttccttccc agttctcgac
     1981 atctttgacc ttggcccgga cgtcgaggaa atacaacgga cgtccgtccg gagccgcccc
     2041 ttcccgggcg gcgaaggaga attccgtccg gcgctcgggc gaaaaaggca gaagcccggc
     2101 gaaatcctcc acccgcatcc ccagccggga gcggcggcgg gcgggctcct ttcccctctc
     2161 ccgggccgtt tcttgggccc ggtatttctc ccagcgggcc cggcgctcgg cgagatactt
     2221 ctccgtgatg tcccgggtga cgccgtcttc cgtccggacg acgcttagaa tgtcctcttc
     2281 gcgccgcccc tcgatcacgg cgacggcctt ggtctggacg gtcgtccgct cgggccggcc
     2341 tttgcggtca agttcaatct gggtactgcg gatggaggcc tgccagtttg atttcggatt
     2401 gaagctttcg aggcgggcgg cgacccgatc gagaagcgtt tcgaccggga gggaggcttg
     2461 ggcggagagg gcggccggga tgagtagaaa agccgccccc gcggccggaa gaatgaagcg
     2521 gcgcatgcgg atctcctgtc ggggattccc aatcatacca gaaatcggtt tgcggaggcg
     2581 tgtctttctc ccggagccgc ggaagggggc ccagccgatc ccggggcggc cggagagtaa
     2641 aaaacagttg acaagcccgg ccgctgccct ctagaatacg actgttccga gagaatcgac
     2701 taaaataata gtatataaag gagaggcgca tgtcgacgtt cctgatcgtg gtcctgggcg
     2761 ttctgggctt tctggccctg gtcatcggtt tcttgggctg gctgttcttc catcagggcg
     2821 gatgctgcgt taaaaagacg gcggccaaga tctcccgcaa gtgcgccgtc taaaaagccg
     2881 gggacgggcc attccccgcc cccgggcgga ggaatccccg gccgggagga aagacggacg
     2941 gtttgatccg ctccgccgga gtctcctccc tgatcggttg gaataagggg cgatgagccg
     3001 ttccctcatt cccaaatcac ttccgcccgt gtatgataaa gatcgtgaga tgacaggagt
     3061 gtgccatggg tccgattaaa aatgaagtcg cggtgatgtt ctccgggggg atcgactccc
     3121 tcctggcggc cgtcctcctt cagaaggaat tcgaaaaggt tcacttgatc acattcgaca
     3181 agggctatct cgaattcggc gtccggaaca atctccccaa tgtcgagcgc ctcaaggccg
     3241 ccttcggcga ggacaagatc acccaccaga tcatcgacca caagccgatc atcaagaaga
     3301 tcgccatgag ctcttttttc aagaaccggg acaagtacaa cgccgagacg cgcatgtgcg
     3361 tcggctgccg cttgagtatg aacaccgggg gcctgctctt cgccctggaa aacgacctcg
     3421 tcggcttcgc cgacggctca aaccgcgagc agatcccgtc gccggccaac ctgaccggga
     3481 cggccgagaa ttatcccagc atcgtcaacg gcgggaagcg gttcgcccag gaatacggcg
     3541 tccatttcat gaccccgacg tacgaatatg gaaaccggga cgagcgccgg gccaagttga
     3601 aggagttggg ctacgatatc gactacttga gcatggatga gagcaaaaag ctctccggcc
     3661 tggtgagcaa aggcctggga aagcggtccc agccgatgtg cttctcgggc tggctcatcc
     3721 actggaagcg caacttattc ggaaaaccgg tcgagcagaa cgaggccatg accgtcgatt
     3781 acgtcaccga gaagcaaaac ggcgtcgtcc gggacgtcat ccaggactat ttcgccgaca
     3841 aggggatcga catcgacgcc ctggtcaagg cccgcaaggc cgatccgcgc ggtcaattca
     3901 ctccggactt cgtttgatcg ccgggggatc agcccgggac cttcctttcg gcgtttccgg
     3961 gatttcctcc gcgcgatctt cccggagccg gagctccggc ggaatccccc gaatgcgtcc
     4021 gacctgattt tttcggccga tccggcggcc ggcgcgccgc t
//
LOCUS       DAOOXH010000143         3961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_27079_length_3961_cov_6.588070, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000143 DAOOXH010000000
VERSION     DAOOXH010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27079_length_3961_cov_6.588070"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1901)
                     /gene="asnB"
                     /locus_tag="PKV64_11135"
     CDS             complement(9..1901)
                     /gene="asnB"
                     /locus_tag="PKV64_11135"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HPN17668.1"
                     /translation="MCGICGLVRTAEGAAFSRELVGRMCGTIVHRGPDDEGVYADGRA
                     GLGNRRLSIIDVKGGHQPLSNEDGTIWIAYNGEAYNFAEIRDELRNRGHVFSTRSDTE
                     TIVHAYEEWGAPAFIERFRGMFAFALWDKRTETLLLMRDRVGVKPLYYTQLPDRTLVF
                     GSELKTILIHPDVRREVEPAALDFYLTLEYVPAPYAMFKNVFKLPAGHFLTWKDGRIE
                     VKRYWDVEPDPDPRPPASFSAAQDELYALLKESVKLRLVSDVPLGAFLSGGIDSSAVV
                     GLMRELGAEPLRTFSIGFTDPTYNELAHARRIAERFQTEHEELVLEPKALELTEKLVR
                     HLDEPLGDFSIFPTYLVSEMARRRVAVALSGDGGDELFAGYEHYLAQRMARWPGVAAA
                     ARAAGAVAGRLPPSKKKKGTWNKIRRFTQAFDQDAGHRHLRWMMFLSDGMKRELYGPA
                     FREAIGGIGALRRREPFPAYFQAMNGFDDRTGELYLDFKTYLVDDIMVKVDRMSMAVS
                     LEAREPLLDHKLVEFAFRLPGDWKLRGGTTKWIFKKTLERLLPRETLYRKKEGFSIPI
                     KHWLATDLRDLMEETLSEKRIREGGWFAYAPIRRMIDAHVAGRENFSHQLWALLVFEI
                     WRGTCL"
     gene            complement(1907..3751)
                     /locus_tag="PKV64_11140"
     CDS             complement(1907..3751)
                     /locus_tag="PKV64_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17669.1"
                     /translation="MLSDKKDALTAVLLISAVFLGFALSADLPALHAGFLAADQATYF
                     SIAQSLAYDHDLEYTKKDLIRYKERFWAGPQGLFLKRVMTPAGEKLYFAKSMAYALFA
                     APFVRIFGANGPIVFHALLVALLLLMGLAFFSLHNRPGLSLLRIMTYLCASVAGAYAL
                     WIAPDFFNLFCVFTVLFLWLYKHRRAEGGTADAPPEKTPGRKSWSGRWTAFLLSDASD
                     YGAAAVAGIVAYAKPPNVAVFGPLLLWTILKKRPGKALVLILVFTVSFGALFGTNVLL
                     TGDWNYQGGERKSFYFSFPYERPELTFDTAQGRLMTADGYLERALRPAKFVPINLFYF
                     IFGRFSGMAWYFFPAFLALVFFFLGRKTLDRWLLLAALTGEILIYVILMPDNFGGGGG
                     SLANRYFMGIYPLFFFLPGADLRPKRLAAEWAAAALLIAPILVSPIQAAHAPADNAKR
                     FPFTCFPVELTLINDLPTNTNPAAFRQRWGEPLFDDRFLYFLNDNFNVKHREENGWWT
                     LGDRKAEFILRTSVPVKEIVFHVLNNPRLENKVTVWVGGRRKTATLGPRARAELRFPV
                     GPGFRVEPARMYRVRIKAAKGSIPYFEDKKSDERRWLGVFFTPELVSP"
BASE COUNT          779 a         1183 c         1322 g          677 t
ORIGIN      
        1 gacgtttctt aaaggcaggt cccgcgccag atttcgaaga ccagcaaagc ccagagctgg
       61 tggctgaaat tttcccggcc ggcgacatga gcgtcgatca tccggcggat cggggcgtag
      121 gcgaaccatc cgccctcccg gatccgtttt tcgctcagag tctcctccat caagtcccga
      181 aggtcggtcg ccagccaatg cttgatcggg atgctgaatc cctctttttt ccgatagagc
      241 gtctcccgcg ggaggagccg ctcgagcgtt ttcttgaaga tccatttcgt cgttccgccg
      301 cggagcttcc agtcgcccgg cagacggaag gcgaattcga cgagcttgtg gtcgagcagc
      361 ggttcccggg cttccagcga gacggccatg ctcatccggt cgactttgac catgatgtcg
      421 tcgacgagat acgtcttgaa gtccaggtag agctccccgg tccggtcgtc gaatccgttc
      481 atcgcctgga agtaagccgg aaacggctcc cggcgccgga gcgctccgat cccgccgatc
      541 gcctcccgga acgccggtcc gtagagctcc cgcttcatcc catccgagag gaacatcatc
      601 caccggagat gccggtggcc ggcgtcctgg tcaaacgctt gggtgaacct ccggatcttg
      661 ttccaggtcc ccttcttttt tttcgagggc ggcaaccggc cggcgaccgc tccggccgcc
      721 cgggcggccg ccgcgacgcc cggccagcgg gccatccgct gggccaagta atgctcgtag
      781 ccggcgaaga gctcatcccc gccgtcgccc gacagggcca cggccacccg ccggcgggcc
      841 atctcggaga ccaggtacgt gggaaagatg gagaaatcgc ccagcggctc atccaaatgc
      901 cggacgagct tttcggtcaa ttccagggct ttcggctcga gaacgagctc ctcgtgctcg
      961 gtctggaatc tctcggcgat ccggcgggca tgggccagct cgttgtaggt cggatcggtg
     1021 aatccgatcg agaatgtccg gagcggctcg gccccgagct cgcgcatcag gccgaccacg
     1081 gccgaggaat cgatgccccc gctgaggaat gcgccgaggg ggacgtcgct caccaggcgg
     1141 agcttgacgg attccttgag aagagcgtac agctcgtcct gggcggcgga gaacgaggcc
     1201 ggcggccgcg gatccggatc gggctcgacg tcccagtatc gtttgacctc gatccggccg
     1261 tctttccacg tcaggaaatg gccggccggg agcttgaaga cgtttttgaa catcgcgtag
     1321 ggggccggga cgtattcaag ggtcaagtaa aagtcgaggg cggccggctc gacctcccgc
     1381 cggacgtccg gatgaatgag gatggttttc agctcggatc cgaaaaccag cgtccggtcg
     1441 gggagttgcg tgtagtagag cggcttgacc ccgacccggt cgcgcatcaa gagaagcgtt
     1501 tccgtccgct tgtcccacaa cgcgaaggcg aacatccccc ggaaccgttc gatgaaggcc
     1561 ggcgcgcccc attcctcgta ggcgtggacg atcgtctcgg tatcggaccg ggtcgagaaa
     1621 acatggccgc ggttccgcag ctcgtcccgg atttcggcga agttgtaggc ctcgccgttg
     1681 taggcgatcc agatcgtgcc gtcctcattg gagaggggct ggtggccccc cttgacgtcg
     1741 atgatgctca accggcggtt gcccagcccg gcccggccgt cggcgtagac cccctcgtcg
     1801 tcgggaccgc ggtggacgat ggtcccgcac atccggccga ccagctcccg ggaaaacgcc
     1861 gctccttccg ccgtccggac cagcccgcag atgccgcaca tcgccatcag ggggaaacca
     1921 gttcgggagt gaagaagacg ccgagccagc ggcgctcgtc gcttttcttg tcctcgaaat
     1981 aggggatgga tcccttggcc gccttgatcc tcacccggta catgcgggcg ggctcgaccc
     2041 ggaatcccgg accgacggga aagcgaagct cggcccgggc gcgggggccg agggtggccg
     2101 tcttccgccg gcccccgacc cagaccgtga ccttgttttc caggcgcggg ttattaagga
     2161 cgtggaagac gatttccttc accgggaccg aagtccggag gatgaattcg gccttgcggt
     2221 cgccgagcgt ccaccagccg ttctcctccc ggtgcttgac gttgaagttg tcgttgagaa
     2281 aataaaggaa gcggtcgtcg aagagcggct cgccccaccg ctggcggaag gcggccggat
     2341 tggtgttcgt cggcaggtcg ttgatcaggg tcagctcgac cgggaagcac gtgaacggga
     2401 aacgcttggc gttgtcggcc ggcgcgtggg ccgcctggat cggcgagacg aggatggggg
     2461 cgatcaggag agcggccgcg gcccattcag cggcgagacg cttcggccgg aggtccgcgc
     2521 cggggagaaa gaaaaacagc ggatagatcc ccatgaaata cctgttggcc agcgaaccgc
     2581 cgccgccgcc gaaattgtcc ggcatgagaa tgacgtagat caggatttcc cccgtcaggg
     2641 cggctaagag cagccagcgg tcgagggttt tccggccgag gaagaagaaa accagggcca
     2701 ggaaagcggg gaaaaaatac caggccatgc cgctgaaccg gccgaagatg aaataaaaca
     2761 ggttgatggg aacgaacttc gcgggccgga gggcccgttc aagatagccg tcggcggtca
     2821 tcagccggcc ttgggcggta tcgaaggtga gctcgggccg ctcgtagggg aagctgaaat
     2881 agaagctctt ccgctcgccg ccctggtagt tccaatcccc ggtcagaaga acgttggtcc
     2941 cgaagagggc tccgaaggag acggtgaata caaggatcag gacgagggct ttgcccggcc
     3001 gtttcttcag gatggtccac aggagcaagg gtccgaagac ggcgacattg ggcggcttgg
     3061 cgtaggcgac gattccggcg accgcggccg cgccgtaatc ggacgcgtcc gagagaagga
     3121 aagccgtcca gcgaccgctc caagattttc gcccgggcgt tttttccggc ggggcgtccg
     3181 ccgttccgcc ctcggcccgc cggtgtttgt acagccagag gaacagaacc gtgaaaacgc
     3241 agaacaagtt gaagaaatcc ggggcgatcc acagggcata tgccccggcg accgaggcgc
     3301 aaaggtaggt catgatgcgc aggagcgaca agcccgggcg gttgtggagg gagaaaaagg
     3361 ccaggcccat cagcaggagg agagcgacga gaagagcgtg aaaaacgatc ggtccgttgg
     3421 ccccgaagat ccggacaaac ggggcggcga acagggcgta ggccatggac ttggcgaaat
     3481 agagcttctc cccggccggc gtcatgaccc gtttcaggaa caggccttgc ggcccggccc
     3541 agaaccgctc cttgtaccgg atgagatcct tcttcgtgta ttccaggtca tggtcgtagg
     3601 ccaggctttg ggcgatggaa aagtaagtcg cctggtcggc ggcgagaaat cccgcgtgaa
     3661 gggccggaag gtcggcggac agggcgaatc ccagaaagac cgcgcttatc aggaggacgg
     3721 ccgttagggc gtcctttttg tccgataaca tggtttctat caataccata atcaaagggg
     3781 acaggtcaaa accaagtccc tctcggtctg ttttttgttt attgagggaa ccgcgccaaa
     3841 taggcggcga tgatccggtc gccccagaca agggcgacgt aagcggcggg ggcgagaaaa
     3901 gacccgaagg gcagggcgaa tttcatgtcc cggccctgga gcttcattag aaggaggccg
     3961 a
//
LOCUS       DAOOXH010000144         3938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_27293_length_3938_cov_4.470255, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000144 DAOOXH010000000
VERSION     DAOOXH010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27293_length_3938_cov_4.470255"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..426)
                     /locus_tag="PKV64_11145"
     CDS             complement(226..426)
                     /locus_tag="PKV64_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17670.1"
                     /translation="MALERETALMLMDLWAFAYTQNPCRKTKNEFESACRLGDALSLP
                     EVEVFRELVSGLDRELVELQAD"
     gene            561..1115
                     /locus_tag="PKV64_11150"
     CDS             561..1115
                     /locus_tag="PKV64_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17671.1"
                     /translation="MSALSIRTRKRLAAAVLVFMIAAPAGLDGVPAPEHEISDLFRRI
                     YEEVRTMPPRPGERFIRQEFFIGEGDDDTDKDIAVSIVLPGENTPPDMIVQITWMERD
                     RRDRRVAVARSTKILRFVLEADGIRWEGDAFGKGEAAVLARGLLKAVRNKKRLLGLQV
                     SPGSVEPREYPCRAAREYHRIAGR"
     gene            complement(1132..1605)
                     /locus_tag="PKV64_11155"
     CDS             complement(1132..1605)
                     /locus_tag="PKV64_11155"
                     /inference="COORDINATES: protein motif:HMM:NF024297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPN17672.1"
                     /translation="MKKGLIFVLVLAAAVGSAATSSLSAEEAEESAVRRAALDYIEGW
                     FEGSVERMDRALHPRLAKRLVEVDPQTGREKVIELTKDIMLEYTKRGGGSRVPADRRL
                     IKITIVDMTETMAMVRSDCSQFIDFLTLAKGEGGAWKIINVLWENVRVDSAKNNF"
     gene            complement(1715..3160)
                     /gene="nhaC"
                     /locus_tag="PKV64_11160"
     CDS             complement(1715..3160)
                     /gene="nhaC"
                     /locus_tag="PKV64_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HPN17673.1"
                     /translation="MPDAVNKQTRSVRPVRLGAALIPILFLIAALSVTIIVLKQDAHL
                     PLIAAAAVAAVVGLCHGHSWASIQDGMIHGITLAMGAILILMIVGIMIGTWISGGIVP
                     AMIYYGLKILSPGLFLAATLIICSIVSLGTGSSWSTAGTVGVALVGVGQGLGIPPAMV
                     AGAIISGAYFGDKMSPLSDTTNLAPAVAGTDIFSHVRHMVYTTTPGYVISLVLYSLLG
                     IRFAGGHIASDKIGLISRTIESHFQIHPLLLLPPILVIVMVVLKIPPLPALLGGTVLG
                     AGFGLVLQGRTLAEVVTTAKSGFVSATGIPLVDDLLTRGGLDSMMSTVALIMCALTFG
                     GIMEKTGILQTVAGALLKRVRRAGSLVATTILSCIGMNAISSDQYMSIVIPGRMYRSA
                     YLKLGLHPKNLSRALEDSATLTSPLIPWNSCGAFMGATLGISPLLYLPFAFLNLSNPL
                     VSIIYGYTGITIHKLTPKQLQILAENPEAAV"
     gene            complement(3244..3543)
                     /locus_tag="PKV64_11165"
     CDS             complement(3244..3543)
                     /locus_tag="PKV64_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17674.1"
                     /translation="MAGEEAAIVFAIQKKIIGKFRDQCATDPRQAMTLESLNIRKRVA
                     VKSLIKKKILVEAPDDRFYLDEEAAEELFKNRRWIFLIVIIFAIIGLLAAYFLNP"
     gene            complement(3631..>3938)
                     /locus_tag="PKV64_11170"
     CDS             complement(3631..>3938)
                     /locus_tag="PKV64_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17675.1"
                     /translation="WYATHRPGKIFRRLMFVELAAEQPQIAAASRFSDAREKLAADPS
                     KKTKSIVFGECLNDVPFRDWAGYRGDVPGGLYAGDRDGLSLWTFPPAVPRTWDRLDG"
BASE COUNT          780 a         1134 c         1283 g          741 t
ORIGIN      
        1 tgggcaaggt ccaccgtctc aatctcatcg atcccctgac gggggaggag atcaagtaaa
       61 gggcgccccc cccgtttgat ccgtcgattt cgttgtcccg gggcccatcg gggccccggg
      121 acgatttaaa aagaggatga ctcggcctcg aggccgagga ggaatcatcg ccgaattgtt
      181 tcacggcgtt cgatctcgcg gccttcagcc cgagttcgaa cagcctcagt cggcttggag
      241 ctcgaccaat tcccggtcga gcccggagac gagctcccgg aagacctcca cctcgggtaa
      301 agaaagggcg tccccgaggc ggcacgcgct ctcgaattca ttcttcgttt tccggcaagg
      361 gttctgggtg taagcgaagg cccagagatc catcagcatc aaggccgttt ccctctcaag
      421 cgccatgttt gttctccttg tcccttttct taaatgcaag gaacgtgcca aggaacgccg
      481 gcccgtgaag ctgtttaaga gtccggattc gttgacgcgg cgcttgttcc ggcataccct
      541 tatccaaagg gactccagcc atgtcggcgc tatcgattcg gacgcgaaaa cggctggcgg
      601 cggcggttct ggtctttatg atcgcggcgc cggccgggct ggatggcgtt cccgctccgg
      661 agcacgagat ttcggacctc ttccgccgga tttacgagga agtccggacc atgccgcccc
      721 gtcccggcga acgattcatc cgccaggaat tcttcatcgg ggaaggcgac gacgacacgg
      781 ataaggatat cgccgtctcg atcgtcctcc cgggggaaaa cactccgccg gacatgatcg
      841 tccaaatcac ttggatggag cgcgaccgcc gcgatcggcg ggtggccgtg gcccggtcga
      901 cgaaaatcct gcggtttgtc ctcgaggcgg acggcatccg atgggaaggg gacgcgtttg
      961 gaaaaggcga agcggccgtc ctggcccgcg gcttgctgaa ggccgttcgg aacaagaaga
     1021 ggctcctggg gctacaggtg tcccccggct ccgtggagcc tcgggaatat ccatgccggg
     1081 ccgcccggga atatcaccgg atcgccggac gttaaattaa tttacattac cttaaaaatt
     1141 gtttttcgcc gaatcaaccc ggacgttctc ccaaaggacg ttgatgatct tccacgcccc
     1201 gccttctccc ttggccaaag tgagaaaatc gatgaactgg gagcaatcgc ttctgaccat
     1261 agccatcgtt tcagtcatat cgacgatcgt gattttgatg aggcgtcgat cggccggaac
     1321 acgggacccg ccgccgcgct tggtgtattc gagcatgatg tccttcgtga gctcgatcac
     1381 tttttcccgg ccggtctggg gatcgacttc aacgagccgc ttggccagcc ggggatgaag
     1441 agcccggtcc atgcgctcga cgctgccttc gaaccagccc tcgatatagt cgagggcggc
     1501 ccggcggacg gccgactctt cggcctcctc ggcggataag gacgatgtcg cagccgaccc
     1561 gacggcggcg gccaggacga gaacgaagat gagacccttt ttcatgaatc gctcctgtta
     1621 tcgaatgatt cggggaatcc gcccaaaaac aggagcaaga agcatgccga tctaggatat
     1681 tccagcgaga gaatccgggc cttgacggaa tcctttaaac cgcggcttcc ggattttcgg
     1741 cgaggatttg gagttgcttc ggcgtcagct tgtggatcgt gatccccgtg tatccgtaga
     1801 tgatggagac aaggggattg gacaggttaa ggaaggcgaa gggaagatag agcaacgggc
     1861 tgatgccgag ggtcgctccc atgaacgccc cgcagctgtt ccaggggatc aggggggatg
     1921 tcagagtggc cgaatcctcg agggcgcggg agagattttt tggatggagg cccaatttca
     1981 ggtaagccga ccggtacatc cggcccggga tgacgatgga catgtactgg tccgaggaga
     2041 tggcgttcat cccgatgcag ctcaggatag tcgtggccac gagggagccg gcccggcgga
     2101 ctcttttgag cagagccccg gccacggtct ggaggatgcc cgttttttcc atgatgcccc
     2161 cgaaggtcag ggcgcacatg atcagggcca cggtgctcat catgctgtcc aggccgccgc
     2221 gggtcagcag gtcgtcgacc aggggaatgc cggtggcgct gacgaatccg gacttggcgg
     2281 tcgtgacgac ctcggccagc gtccgtcctt ggaggaccag gccgaagccc gccccgagaa
     2341 cggtcccgcc gaggagggcg ggaagcggcg ggattttcag gacgaccatg acgatgacca
     2401 ggatcggggg gagaagcaga agcggatgga tctggaaatg cgactcgatc gtccgggaga
     2461 tcaggccgat cttgtccgag gcgatgtggc cgccggcgaa ccgaatcccg agcagactgt
     2521 acagaacgag ggagatcacg tatcccggcg tcgtcgtgta gaccatgtgg cggacatggc
     2581 tgaagatatc ggttccggcg acggcggggg ccaaattggt cgtgtcggag aggggggaca
     2641 tcttgtcgcc gaagtaagcc ccggaaatga tggctccggc gaccatggcc gggggaatgc
     2701 ccagcccttg tccgaccccg accagggcga cgcccaccgt tcccgccgtc gaccaggacg
     2761 atcccgttcc cagggaaacg atggaacaga tgatgagcgt ggccgcgaga aagaggccgg
     2821 gcgagagaat cttgagcccg taataaatca tggccggcac gatgccgccc gagatccagg
     2881 ttccgatcat gatcccgacg atcatcagga tgagaatcgc ccccatggcc agagtgatgc
     2941 cgtggatcat cccgtcctgg atggaggccc agctgtgtcc gtggcacaac ccgacgacgg
     3001 cggccacggc ggcggccgcg atcaagggga ggtgggcgtc ctgtttcagg acgatgatcg
     3061 tgacggaaag ggcggcgatg aggaagagaa tggggatgag ggcggccccg agacgaaccg
     3121 gccggaccga tcgcgtttgc ttattcacgg cgtcgggcat gaagagccat tctaaataaa
     3181 gggcggggaa aaggcaacct ttgggacgcg gccgggccgt cccggtcgaa aacgcgcggc
     3241 ggcctacgga ttgaggaaat aggccgcaag aaggccgatg atcgcgaaga tgatcacgat
     3301 caggaaaatc cagcgtctat ttttaaagag ctcctcggcc gcttcctcgt ccaggtagaa
     3361 acggtcgtca ggcgcctcga cgaggatctt tttcttgatc aggctcttga cggcgacccg
     3421 cttcctgatg ttcaacgatt ccaaggtcat cgcttggcgc ggatcggtcg cgcactgatc
     3481 ccggaatttg ccgatgattt tcttctggat cgcgaaaacg atcgcggctt cttctccggc
     3541 catgtcttct tctccttctg aatccttata cggatttccc cccggcgggt tgtctcttcg
     3601 tcgggatgcg ccgcggccgc cgcggcggtc tcacccgtcc agacggtccc aggtccgggg
     3661 aacggccggc gggaatgtcc agagggacag gccgtcccgg tcgccggcgt agaggccgcc
     3721 gggaacgtcg ccgcggtatc cggcccagtc ccggaaagga acgtcgttga gacattcgcc
     3781 gaagacgatg ctcttggtct ttttggacgg atcggcggcc agcttctccc gggcgtcgct
     3841 gaagcggctg gcggccgcga tctggggttg ttccgcggcc agttcgacga acatcagccg
     3901 gcggaagatc tttcccgggc ggtgggtcgc gtaccagg
//
LOCUS       DAOOXH010000145         3919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_27488_length_3919_cov_5.909420, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000145 DAOOXH010000000
VERSION     DAOOXH010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27488_length_3919_cov_5.909420"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1800
                     /locus_tag="PKV64_11175"
     CDS             88..1800
                     /locus_tag="PKV64_11175"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HPN17676.1"
                     /translation="MTPPLDFYELRKRQKIRSLAVAALLFLFYFLAVGLLLLAAGLTA
                     GFFAVRPVFAGSGFWTKFIVLDVLLAAAVAAFHYLDARKNGPAYLIKRLSAKPPDPAD
                     RYHRMFADAVAGVRLAAGLPAVDPLILPTFSLNSLALILPGGRPAVLVTEGLLADCAR
                     DEVEAVVAHEMGHIARGDTTLLTLVCGLSSFFERLRDACESEVEEAAPVLGRRNRTGA
                     AAGLLYAASAAGAAVIRFLEVFVSRERELLADAAAVEFGRSPMALARAVYKAQARNVF
                     IGDFSQAFAPLFLIAPSAPPEDGAKAPRWSSTHPAFEERIRLLAAMARRTPEAVAGQV
                     RDAAEARKKARTIAAPAAPPPAGRETEAPPERRDWMMIRPGRGETGPAALEELIEADG
                     FSPSVLVRNLADGLDGRAGDFPEIRDALRRRRQGRPVVMRRLGLCPRCRIPLGDTHYE
                     GVPAGLCRRCGGKSIDPRFMNRIIARREIGFSADLVKKAEAFERERLLNPAARRKSAD
                     PRKTRLICPSCGCPMIPRPFNYQYVVPVDKCGSCGRIWFDADELETLQILIERARTDE
                     KERR"
     gene            1797..2498
                     /locus_tag="PKV64_11180"
     CDS             1797..2498
                     /locus_tag="PKV64_11180"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HPN17677.1"
                     /translation="MKPRIILHNAVSADGRMDWFEANLETYYELAGRFDEDVTLAGSG
                     TMLQSPLGEALAREPEEIPEPAEIDPADARPILAVPDSRDRIKNWTSLRTLPFWRDVL
                     ALSSSTAPPAQIERFKRQGVPYFLAGRTRVDLGAALAAIAGRYNAKTIRVDSGGTLNG
                     VLLRLGLVDEISLLVHPQLVGGISPRTIFRAEDLTSAEDVIRLELAANETLSGGLVWL
                     RYNVVTKRPPGLPED"
     gene            2595..>3919
                     /locus_tag="PKV64_11185"
     CDS             2595..>3919
                     /locus_tag="PKV64_11185"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPN17678.1"
                     /translation="MNILVVSDTPETFTLLEKTVVPKGFTIFYSSNRDNIKSVIRRRG
                     IRIIILRLSPEETDDLRPLREIRNFDPLIEVILAGPPLPVNKTVEAVNLGAADYVVEP
                     FDGEDILAILDRIREKADLRRQTYRLEKQLAEKYVFEGMVGRSAVMLELFSLIERLAK
                     FSSPVLIQGATGTGKEMVARALHRLSPRRERKLVVCDCTSIPESLFESELFGYERGAF
                     TGADKAKPGIFEDSDGGTLFLDEIGDIPPIIQTKLLRVLEEHRFRPLGSNAFIEIDVR
                     VICATNKNLRALISEGLFREDLFHRINLAEINLPALRERRDDILMLANHFLETSSRKF
                     KKDVRGISQRAKRILANYDYPGNVRELENLIERAVMLSTEAFVDIKDLPEPVVIASER
                     AENDEREARPYPFTQMSLEDIEKKHIGEILKAAGHNKVKAAKMLGLTRPA"
BASE COUNT          681 a         1340 c         1225 g          673 t
ORIGIN      
        1 gaaaaaacag attatcacag cccggacgtt tcttcaaagc gccggaaaga cttgcggccg
       61 gagacggctt cgtttacgat ggcggcgatg accccccctc tggatttcta tgagctgagg
      121 aaacggcaga agatccgcag cctcgccgtc gccgccctgc tcttcctctt ttatttcctg
      181 gccgtcgggc ttctcctcct cgccgccggg ctgacggccg gcttcttcgc cgtccggccc
      241 gttttcgccg gctcgggatt ctggacgaaa ttcatcgttc tcgacgtcct cctggccgcg
      301 gccgtcgccg cttttcatta tctcgacgcc cggaaaaacg ggccggctta cttgatcaaa
      361 aggctttccg ccaagcctcc cgatccggcc gaccgctacc accggatgtt cgccgacgcc
      421 gtggccggcg tccgcctcgc ggccgggttg ccggcggtcg atcccctgat tctcccgacg
      481 ttttccctca actcgctcgc cttgatcctc ccgggcgggc ggccggccgt cctggtcacc
      541 gaaggcctgt tggccgactg cgcccgggat gaagtcgagg cggtcgtcgc ccacgaaatg
      601 ggccacatcg cccggggcga cacgacgctc ctgaccctgg tctgcggcct ttcctctttt
      661 ttcgagcggc tccgggacgc ctgcgagtcc gaggtcgagg aggccgcccc ggtcctcggc
      721 cgaagaaacc gaacgggcgc ggccgccggc ttgctgtacg ccgcctccgc cgcgggggcg
      781 gcggtcatcc gttttctcga ggtctttgtc tcgcgggaac gggagctttt ggccgatgcc
      841 gcggccgtcg aattcgggcg cagcccgatg gccctggccc gggccgttta caaggcccaa
      901 gcccgaaacg tcttcatcgg cgatttcagc caggccttcg cccccctttt cctgatcgcg
      961 ccgtcggcgc cgccggagga cggagcgaaa gcgccccgct ggtcaagcac ccatccggcc
     1021 ttcgaggagc ggatccggct tctggccgcc atggcccgcc ggacgcccga ggccgtggcc
     1081 ggccaggtga gagacgccgc cgaggcgcgg aaaaaagccc ggacgatcgc ggccccggcc
     1141 gcgccgcctc cggccgggag ggagacggaa gcgccgccgg aacgaaggga ctggatgatg
     1201 atccggccgg gccgcggcga aaccggcccg gcggctctcg aggaattgat cgaggcggac
     1261 ggattttctc cgtccgtcct cgtccgaaac ctggccgacg gcttggacgg ccgggccggc
     1321 gattttccgg aaatccggga cgccctccgt cggagacgcc agggccggcc cgtggtgatg
     1381 cgccgcctcg gcctctgccc ccgctgccgg attccgctag gcgacaccca ttatgagggc
     1441 gtaccggccg gcctctgccg ccgctgcggc ggcaaatcga tcgatccccg gttcatgaac
     1501 cggatcatcg cccgccggga gatcgggttt tcggccgatc tcgttaaaaa agccgaagct
     1561 ttcgagcggg agcgccttct caatcccgcc gctcggagga aatccgccga tccccggaaa
     1621 acccgcctga tctgcccttc ctgcggctgc ccgatgattc cccggccgtt caactatcaa
     1681 tacgtcgtcc ccgtcgacaa atgcggctcc tgcggccgga tctggttcga tgccgacgag
     1741 ctcgagaccc tccagatcct catcgagcgg gcgcggacgg atgaaaagga gagacgatga
     1801 aaccccgcat cattctgcac aacgccgtga gcgccgacgg ccggatggat tggttcgagg
     1861 cgaacctgga gacctattac gagctggccg gccggttcga cgaggacgtc accctggccg
     1921 gcagcgggac gatgctccaa agccccctgg gcgaagcgct cgcccgcgag ccggaggaaa
     1981 tccccgaacc ggccgagatc gatccggccg atgcgcgccc gatcctggcc gtcccggaca
     2041 gccgcgaccg catcaagaac tggacgtccc tgcggacgct tccgttctgg cgcgacgttc
     2101 tcgccctctc gtcctcgacg gcgccgccgg cccagatcga gcggttcaag cgccagggcg
     2161 tcccttattt cctggccggg cggacccggg tcgatctcgg agcggccttg gccgcgatcg
     2221 ccgggcgcta caacgccaaa acgatccggg tggattcagg cggaacgctg aacggcgtcc
     2281 tcctccgcct gggcctggtg gacgaaatca gcctccttgt ccatccccag ctcgtcgggg
     2341 ggatctcccc ccggacgatt ttccgggccg aggacctgac atcggccgag gacgtcatcc
     2401 ggctggagct ggcggccaac gagacgctgt ccggcggtct cgtctggctt cgctacaacg
     2461 tcgtcaccaa acggcctccc ggccttccgg aagattgacc gtccctccgt tttcttgaca
     2521 ctcagcgcgg gccggcgata tagtggcgcc atgagcggga ggaaaacgaa tccgcgggcc
     2581 gggacgggcg attcatgaac attctcgtcg tctccgatac cccggaaacc ttcacccttc
     2641 tggaaaagac cgtcgtcccg aaagggttca cgatcttcta ttcctccaac cgggacaaca
     2701 tcaagtccgt catccgccgc cgcgggatcc ggatcatcat cctccgcctg tctccggagg
     2761 aaacggacga ccttcggccg ctgcgggaga tccggaattt cgatcccttg atcgaagtca
     2821 tcctggccgg tcctcccctt cccgtgaaca agaccgtcga ggccgtgaat ctcggagccg
     2881 ccgattacgt ggtcgagccg ttcgacggcg aggacatcct ggccattctc gaccggatcc
     2941 gggagaaggc cgacctccgg aggcaaacct accggctgga gaaacagctg gccgaaaaat
     3001 acgtgttcga gggcatggtc ggccggagcg cggtcatgct cgagctgttc tcgctgatcg
     3061 agcggctggc caagttttcc tcgcccgtcc tcatccaggg agccacgggg accgggaaag
     3121 agatggtcgc ccgggctctc caccgtctga gcccccggcg ggagcggaag ctcgtcgtct
     3181 gcgactgcac ctccatcccg gaaagccttt tcgagtccga gctcttcggc tacgagcggg
     3241 gggcgttcac cggagccgac aaggcgaagc ccggcatatt cgaggacagc gacggcggga
     3301 ccctgttcct ggacgaaatc ggagacattc ccccgatcat ccagacgaag cttctccggg
     3361 ttctggagga gcaccggttc cggccgctgg gctcgaacgc ttttatcgag atcgacgtcc
     3421 gggtgatctg cgccacgaac aagaaccttc gggccttgat ctccgaggga ttgttccgcg
     3481 aggatctctt ccaccggatc aacctggccg agatcaacct gccggcgctc cgggaacggc
     3541 gggacgacat tctcatgctg gccaatcatt tcctggaaac gagcagccgg aaattcaaga
     3601 aggacgtccg gggcatctcc cagcgggcca aaaggatcct ggccaattat gattacccgg
     3661 gaaacgtccg ggagctggaa aacctgatcg aacgggcggt catgctgagc accgaagcgt
     3721 tcgtcgacat caaagacctc ccggaaccgg tcgtcatcgc ctcggaacgg gcggaaaacg
     3781 acgagcgcga ggcgaggcct tatcccttca cccaaatgag tcttgaggat atcgagaaga
     3841 agcacatcgg cgagattctc aaggcggccg ggcataacaa ggtcaaggcc gcgaaaatgc
     3901 tcggcctgac ccggccggc
//
LOCUS       DAOOXH010000146         3909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_27590_length_3909_cov_8.132330, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000146 DAOOXH010000000
VERSION     DAOOXH010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27590_length_3909_cov_8.132330"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="PKV64_11190"
     CDS             complement(<1..1157)
                     /locus_tag="PKV64_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPN17679.1"
                     /translation="MNPFIKSSLKSLALGTVCALAFGLLTAPAFLRADEEATTAAEED
                     QAAKITEEIQVVGKAPKVQPVSTVTTIAASLIEQYAPRDLSEAVQYAPGVNITTGSKR
                     EATLKLRGMDSRRIVLLIDGIPSYEPYYGSFDLKTVSAAGLESIQITKGPSSVLYGPN
                     TLAGIVNVITRRPGDNPFLAVQGSLGAQKTYSAGFDGGFRWNRFSLVGDVSWQNSDGF
                     GYPDPDTGERTDLMNTDYERFNLNAKAYYAPSDDSEIMINGGIYQSNYGMPPTLGVQK
                     ARYWYFKNWDRYTLNAGGFTSLGEGAVLRFRAFYVNYRNTMDQFKDKEMAKRQFESTF
                     DNYVYGAFAMSEFKLASWNTLKASLNFQRDVARTQDDIGLPFDEYDQGTFSA"
     regulatory      complement(1147..1266)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(1327..2943)
                     /locus_tag="PKV64_11195"
     CDS             complement(1327..2943)
                     /locus_tag="PKV64_11195"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HPN17680.1"
                     /translation="MPRRRSSPAAVAVLAAALISAACAGNNGKPENAVFITLDTFRAD
                     HLSALSRGRAATPVLDALAARGILYENAWSPIPITLPAHKSIFFGRPPHLLSTYNNGQ
                     IVASRRDHPSFVQAFRKKGFDTAAFVSLGVLTREFGTAEGFEHYEDRFPDERWYLTAE
                     EVNRRVIPWLEARGQKPFFLWVHYSDPHDPYAPPGMPDDLALSLNGRKAGSFRIGDYA
                     WNTVTLDLRAGENILAFETMNPYQPDPSKFSAWLDPLEFGPDEAMKDVFWEHTERWFI
                     IPETGTRFLRNGAFLTLRCPTARRVTMRFRGQLYLTTTAVEDLYRKEVEYLDGKVGEL
                     LAALDRLGLRDETAVLVAGDHGEGLGEYRLESGAIHYGHINFLEKVYLHVPLIISVPG
                     GPGKGTRRAEPVTLLDIAPTFLAMFELEGIRGLPGRNLLALPAGAGAPVFAATYRPES
                     DRNRFALIEAPWHMILSPEQRMRKMYDMSSDPGERTDIWETAAMPRRVGLDLEKRLGD
                     FARRALAGKKDRTLDPKTAEMLKSLGYLGN"
     gene            complement(2947..>3909)
                     /locus_tag="PKV64_11200"
     CDS             complement(2947..>3909)
                     /locus_tag="PKV64_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPN17681.1"
                     /translation="RHAVELDLKAGENVLVFEVRNPFQSNPDRPAARLDEIRLRPGPE
                     TAGVSWEPGGRWISFPGKTARFFKDGATIVLRAPSARKVTMEFFGQLFLPIPATRTQY
                     AREVEYLDGKIGELLAVLDRLGLRDSTAVIAVGDHGEGLGEFANEAGEPHCGHIKFLE
                     KVYLHVPLIIALPGDRRAGTRRTEPVTLLDIAPTFRTLFGLDGLRDLPGRDLLSLPEG
                     ASIPVFAETYRPESDRNRFALYDGPWQMILNPELGRHRMYNLSDNPAETKDLWDTEAV
                     PPAVRIDFEKRLGDFARRILSEKTDASIDPKAEEMLRSLGYIGN"
BASE COUNT          712 a         1186 c         1300 g          711 t
ORIGIN      
        1 gcggaaaacg tcccctggtc gtactcgtcg aacgggagac cgatatcgtc ctgggtccgg
       61 gccacgtccc tctgaaaatt caggctggct ttcagcgtgt tccaagaggc gagcttgaat
      121 tcggacatgg cgaacgcgcc gtaaacgtag ttgtcgaacg tgctctcgaa ctgccgcttg
      181 gccatttcct tatccttgaa ctggtccatc gtgttgcggt agttcacgta aaacgcccgg
      241 aagcggagaa cggcgccctc gccgagcgag gtgaagcctc cggcgttcag cgtgtaccgg
      301 tcccagttct tgaaatacca gtaccgggct ttctggacac ccagggtcgg gggcatgccg
      361 taattcgatt ggtagattcc gccgttgatc atgatctcgg aatcgtccga gggggcgtag
      421 taggccttgg cgttgagatt gaagcgctcg taatccgtgt tcatcaagtc ggttctttcc
      481 ccggtgtccg ggtccggata accgaagccg tccgaattct gccaagagac gtcgccgacc
      541 agcgagaacc ggttccaccg gaagccgccg tcgaagccgg cgctgtaggt cttctgggcg
      601 cccaggctgc cctggacggc caggaaggga ttgtctccgg gacgccgggt gatgacgttg
      661 acgatgccgg ccagcgtgtt cggcccgtac aggacggagg acgggccctt ggtgatctgg
      721 atcgattcga gcccggcggc cgaaaccgtc ttgagatcga acgatccgta gtacggctca
      781 tagctgggaa tgccgtcgat gagcaggacg atccggcgcg agtccatgcc ccgcagcttg
      841 agcgtcgcct cgcgcttgct gccggtcgtg atgttcacgc ccggggcgta ctgcacggcc
      901 tcgctgagat cgcggggcgc gtactgctca atcagggacg cggcgatggt cgtcaccgtc
      961 gaaacgggct ggaccttggg ggccttgccc acgacctgga tttcctcggt gattttggcc
     1021 gcctgatctt cctcggccgc cgtcgttgct tcctcgtccg cgcgcaggaa ggcgggcgcc
     1081 gtcaggagtc cgaaagccag ggcgcagacc gtccccaggg caagactttt taaagatgat
     1141 tttataaagg gattcatgga tcctcctttt caagcacgga aggcgcggcc gtttccggcg
     1201 gcgcccgttg gtctcgaggc ttggacgcgg cgctttagcc tcttcgactc ggagagaagg
     1261 ggaattatac ccaggccagc ggcgaagtca agaaagggcc cgccggaaag gggctccggc
     1321 cgggcctcaa ttcccgagat agcccaggga cttcagcatc tccgcggttt tcgggtcgag
     1381 ggtcctgtct ttcttcccgg cgagggcccg gcgggcgaag tcgccgagcc gtttctcgag
     1441 atcgaggccg acgcggcgcg gcatggccgc cgtttcccag atatcggtcc gctcccccgg
     1501 atcggacgac atgtcgtaca tcttccgcat cctctgctcg ggagacaaaa tcatatgcca
     1561 gggcgcctcg ataagggcga aacgattgcg atcggactcc ggccggtacg tcgcggcgaa
     1621 taccggcgca cccgcccccg ccggcagcgc caggaggttc cggccgggaa ggccccggat
     1681 cccctccagc tcgaacatcg ccagaaaagt cggggcgatg tccaaaagag tgaccggctc
     1741 ggcccgccgc gttcccttgc ccgggccgcc gggaacggag atgatcaggg ggacatggag
     1801 atagactttt tccaggaagt tgatatggcc gtaatggatc gccccggatt cgagccggta
     1861 ctcccccagc ccttcgccgt ggtccccggc gacgaggacg gccgtttcat cccgcaagcc
     1921 gagacggtcc agggcggcca agagctcccc gactttcccg tcgaggtact cgacttcctt
     1981 ccggtacaga tcctcgaccg ccgtcgtggt cagatagagc tggccccgga accgcatcgt
     2041 cacccggcgg gcggtcggac agcggagggt gaggaacgcc ccgttcctta aaaaacgggt
     2101 tcccgtctcg ggaatgatga accatcgctc ggtatgttcc cagaaaacgt ctttcatcgc
     2161 ctcgtccggg ccaaattcga gcgggtcgag ccaggccgaa aacttggagg gatcgggctg
     2221 gtagggattc atggtctcga acgccaggat gttttccccg gcccgaagat cgagagtgac
     2281 ggtgttccag gcgtagtcgc cgatccggaa ggaacccgcc ttccggccgt tgaggctcag
     2341 ggcgaggtcg tccggcatgc ccggcggggc gtaggggtcg tgcgggtcgg aataatgaac
     2401 ccagaggaaa aacggctttt ggccgcgggc ctccagccac ggaatgaccc ggcggttgac
     2461 ctcctcggcc gtcagatacc agcgctcatc cggaaatcga tcctcataat gttcaaatcc
     2521 ctcggccgtg ccgaactcgc gggtcaaaac gcccagcgaa acgaaagccg ccgtgtcgaa
     2581 gcccttcttc cggaaggcct ggacgaagga cgggtgatcg cggcgggagg cgacgatctg
     2641 gccgttgtta tacgtcgaga ggaggtgggg agggcgcccg aaaaagatcg atttgtgggc
     2701 gggcagcgtg atcggaatcg ggctccaggc gttctcgtag agaatgcccc gggcggccag
     2761 ggcgtccagg acgggcgtgg ccgcccgtcc ccgggaaaga gcgctcaagt ggtcggcccg
     2821 gaacgtgtcg agggtgatga agacggcgtt ttcgggtttc ccgttgtttc ccgcgcaggc
     2881 cgccgaaatc aaggcggcgg cgagaacggc gacggcggcc ggagaggatc gtcgtcgggg
     2941 catgagctag ttcccgatat accccagcga ccggagcatc tcctcggcct tgggatcgat
     3001 cgaggcgtcc gtcttttcgg ataggattcg gcgggcgaag tcgccgagcc gtttttcgaa
     3061 atcgatcctc acggccggcg gcacggcctc cgtatcccaa aggtccttgg tttccgcggg
     3121 attatccgac aggttgtaca tccggtgccg gccgagctcg ggattcagga tcatctgcca
     3181 aggaccgtca tagagggcga agcggttccg atcggattcc ggccggtagg tttcggcgaa
     3241 caccgggatc gacgcgccct cgggaagaga gagaagatcg cggcccggca ggtcccggag
     3301 tccgtccagg ccgaagagcg tccggaacgt gggggcgatg tccaagaggg tcaccggctc
     3361 ggtccgccgg gttccggccc ggcggtcgcc gggaagggcg atgatcaagg gaacgtgaag
     3421 atagactttc tcgaggaact tgatatgccc gcagtgaggt tcgccggcct cgttggcgaa
     3481 ttcccccagc ccttcgccgt ggtcgccgac ggcgatgacg gccgtgctgt cgcgaaggcc
     3541 gagacggtct aacacggcta aaagctcgcc gattttcccg tccagatatt cgacctcgcg
     3601 ggcgtactgc gtccgggtgg ccgggatcgg gaggaacaat tgtccgaaga attccatcgt
     3661 caccttgcgg gccgacggcg cccgcagaac gatcgtcgcc ccgtccttga aaaagcgggc
     3721 cgttttcccg ggaaaggata tccagcgtcc cccgggctcc caagagacgc ccgctgtctc
     3781 cggaccggga cggagccgga tctcgtccaa acgggccgcc ggacggtccg gattcgattg
     3841 aaacggattg cgcacttcaa aaacgaggac attctccccc gccttgaggt cgagctcgac
     3901 ggcatgccg
//
LOCUS       DAOOXH010000147         3798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_28710_length_3798_cov_3.981833, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000147 DAOOXH010000000
VERSION     DAOOXH010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28710_length_3798_cov_3.981833"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1149)
                     /locus_tag="PKV64_11205"
     CDS             complement(148..1149)
                     /locus_tag="PKV64_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPN17682.1"
                     /translation="MRKAKKREMTAGEGPLFAAGVDVGGTKVSTALFDSGGRMFARAK
                     QPLDRGSGEAAAGQVARVLRELGEEAVRAGGRVKAAAVCIPGIVYQADGLVWAPNVPG
                     WIHFPLQGRLEGAVPFPVLIESDRAAYVSGEAWRGAARGARDAVFLAVGTGIGAGIIS
                     GGRLIRGAEDISGAVGWFALRPEFREEYAEMGCFEAEASGTGVGRRARRLLESGVSSE
                     MIRLAGGRIADVTAETVAAAARLNDPPARAIMAETASTLAMGIANIVSLLNPKVIVLG
                     GGLFQAADLLLEPIRKEIKKWAQPLAAKDLEVAVSTLGEDAGLYGCGKLAWDLSKGE"
     gene            complement(1146..3461)
                     /locus_tag="PKV64_11210"
     CDS             complement(1146..3461)
                     /locus_tag="PKV64_11210"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPN17683.1"
                     /translation="MPNVSKRIPLLILAAGAVLAAAAATGFADEKNKDFFPMSVWYAG
                     GKARAPMLETVAPDSAAAWRKDLRQIKDLGFNTVRTWIEWTACEKDEGRYDFSALKTV
                     VDLAGEMGLRVVVQVYIDSAPDWVGRRFPEARFVSSNGVAVQSQAAPGFCFDHPGVRE
                     KILRFFTEAAKAVKGKPAFYGWDLWSEPHIINWAEVYYVGNLEHVQFCYCPATQARFR
                     EWLKARYGTIDGLNKAWYRTFRDWAEVEPPRFGTILTYTDYIDWKEFISDKLAADLKL
                     KAEAVKAVIPESVVTSHSATPGLFSRQDWSGTPDDRKMSDSVDYYGTSIYPKHAWTLR
                     PWPPFYRAAGLDFVRSMALRNGGFYVGELQAGYGVFGMKMSVPVVGADLRDWMWSTIA
                     YGARAVNIYAYYPMSSGYEGGGYGLVELDGKVTERAVEAGRIAGVVTAHKDLFLKSLP
                     APAEIAVVYNPLSHMVGGQQSFTGEGQAVGYNTLSESLQGVHRAFYERHVPVDFLHVR
                     DLAPERWKRYKLLIVPYPVMMSREHVGRLIEYVRDGGTLVAEARCGWVDERGFSSPVI
                     PGGGLDEVFGCRETELLPLAKTSAVTIAAADEALPLLKPGDALDAVFFEEALDVFGPR
                     SRVLATFADGKPAMVASSFGRGKAVLVGSFPGSAYHHFKNPANGKFFAGLADWAGVRK
                     PVEVSIPDPDALVEGRILEGADFRILIAFNRGERKTKASFALPGFPKDPAVRDLESGK
                     AEAFRRDGETVVVEKEMAPGGVWTVVFEKKK"
     gene            complement(3445..>3798)
                     /locus_tag="PKV64_11215"
     CDS             complement(3445..>3798)
                     /locus_tag="PKV64_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17684.1"
                     /translation="CPALGLPFQTSGEVSFTEVQNRLANGWTSSRDVCARVPLLQSPG
                     GGAIVSYEDVLSVRSKCRYALNNGVAGFIIWELSHDRWRGQSVLLEAVGDFFLHRTNL
                     PRRGEDKESRHAERF"
BASE COUNT          639 a         1304 c         1238 g          617 t
ORIGIN      
        1 gcccgctgcc gaacatatag acgcgcccgc cccggcggat ggaagcggcc atcgccgcgg
       61 ccgcctctcc gatgcggccc gtctcccggg ttttgatctc ggcgaggatt tccccgattt
      121 tttccagata ccgcgtggac gacatgatta ttctcctttc gagagatccc aagccagctt
      181 gccgcagccg tacagcccgg cgtcctcgcc gagcgtcgaa accgcgacct cgaggtcctt
      241 ggccgcgagc ggctgggccc atttcttgat ctccttccgg atgggctcca ggaggagatc
      301 cgcggcctgg aacaggccgc cgccgagaac gatgactttg ggattgagaa ggctgacgat
      361 gttggcgatc cccatggcca gggtggaggc cgtttcggcc atgatcgccc gggccggagg
      421 gtcgttgagc cgggcggcgg cggccacggt ctcggccgtt acgtcggcga tccggccgcc
      481 ggccaggcgg atcatctccg aggacacgcc cgactccagg aggcggcggg cccggcggcc
      541 cacgccggtc cccgaagctt cggcctcaaa gcaccccatt tccgcgtatt cctcccggaa
      601 ctccggccgg agggcgaacc accccacggc gccggagata tcctcggccc cgcggatcag
      661 ccgcccgccg gagatgatgc ccgccccgat gccggtcccg acggccagga agacggcgtc
      721 ccgggcgccc cgggccgccc cgcgccaagc ttcgccggag acatacgccg cccggtcgct
      781 ttcgatcagg accggaaagg ggaccgcgcc ttccagcctc ccttggagag ggaaatggat
      841 ccagccgggg acgttgggcg cccagacgag gccgtcggct tgatacacga tccccgggat
      901 gcagaccgcc gcggccttca cccggccgcc cgcccggacg gcctcctccc caagctcccg
      961 gaggacgcgg gcgacctggc cggccgccgc ctcgccgctt ccccgatcga ggggctgctt
     1021 ggcccgggcg aacatccggc cgccggaatc aaacaaggcc gtggagacct tggtcccgcc
     1081 gacatcgacg ccggcggcga agagcgggcc ttcaccggcc gtcatctccc gctttttagc
     1141 ctttctcatt tctttttttc gaaaacaacc gtccaaacgc cgccgggcgc catctctttt
     1201 tcgacgacga cggtttcgcc gtcccgccgg aacgcctcgg ccttgccgga ttcgaggtcg
     1261 cgcacggcgg gatccttcgg gaacccggga agagcaaagg aagccttcgt cttccgctcg
     1321 ccccggttga aagcgatcag gatccgaaaa tccgccccct cgagaatccg tccctcgacc
     1381 agggcgtccg gatccggaat cgaaacctcg accggcttcc gcactccagc ccagtccgcc
     1441 aggccggcga agaatttccc gttggccggg ttcttgaaat gatggtaggc ggatcccggg
     1501 aaggatccga cgagaacggc tttcccccgg ccgaacgagg aggcgaccat cgccggcttg
     1561 ccgtcggcga acgtcgccag gacccggctc cggggtccga agacgtccag ggcttcctcg
     1621 aaaaagacgg cgtccagcgc gtccccgggc ttgaggagcg gcagggcctc gtcggccgcg
     1681 gcgatcgtca ccgcggaggt tttggccagc ggcaggagct cggtttcccg gcagccgaac
     1741 acctcgtcca agccgccgcc ggggatgacg gggctggaaa agccgcgctc gtcgacccag
     1801 ccgcagcggg cctcggcgac gagcgtcccg ccgtcccgga cgtattcgat gagccgcccg
     1861 acatgctccc gcgacatcat gaccggataa gggacgatca ggagcttgta gcgcttccac
     1921 cgttcaggcg cgagatcgcg gacgtggagg aagtcgaccg ggacatgccg ctcataaaaa
     1981 gcccggtgga cgccttgaag ggactcgctg agcgtgttgt atccgacggc ttgaccttcg
     2041 ccggtgaacg actgctggcc gccgaccatg tgcgacaacg gattgtagac gaccgcgatc
     2101 tcggccgggg ccggcaggct cttcaaaaag agatccttgt gggccgtgac aacgccggcg
     2161 atgcggccgg cctcgacggc ccgctcggtg acctttccat cgagctcgac cagtccgtag
     2221 ccgccgccct cgtagccgga gctcatgggg tagtaggcgt agatgttgac ggcccgggcg
     2281 ccgtaggcga tcgtgctcca catccagtcg cgcaagtccg ccccgacgac cgggacgctc
     2341 atcttcatcc cgaacacacc gtagccggcc tgcagctcgc cgacgtagaa tccgccgttt
     2401 cggagggcca tcgagcggac gaaatcgagc cccgcggccc ggtagaacgg cggccagggc
     2461 cgcagggtcc aggcgtgctt gggatagatc gacgtcccgt aataatcgac gctgtcggac
     2521 atcttccggt cgtcgggcgt cccggaccaa tcctgccggc tgaacaggcc gggcgtggcc
     2581 gagtggctcg tcaccacgct ctcggggatc acggctttga cggcctcggc tttaagcttg
     2641 aggtcggcgg ccagcttgtc cgagatgaat tccttccagt cgatgtaatc cgtgtacgtg
     2701 aggatcgttc cgaagcgcgg cggctcgacc tccgcccagt cgcggaacgt ccggtaccag
     2761 gccttgttca agccgtcgat cgtcccgtag cgggccttga gccactcgcg gaaccgggct
     2821 tgggtcgccg ggcagtagca gaactggacg tgttcgagat ttccgacgta atacacctcg
     2881 gcccaattga tgatgtgcgg ctcgctccag aggtcccagc cgtagaaggc gggtttcccc
     2941 ttgacggcct tggccgcctc ggtgaagaac cgcaggatct tctcccggac gccgggatgg
     3001 tcgaagcaga agcccggggc cgcctgggac tggacggcca ccccgttgga ggaaacgaac
     3061 cgggcttcgg ggaaccggcg cccgacccag tccggggcgg agtcgatata gacttggacg
     3121 acgacccgga gccccatttc cccggccagg tcgacgacgg tcttcaaggc gctgaaatcg
     3181 taccggccct cgtccttttc acaggccgtc cactcgatcc acgtccggac cgtgttgaag
     3241 ccgaggtcct tgatctgccg caggtccttg cgccaggccg cggccgaatc gggggcgacg
     3301 gtctccagca tcggggcccg ggctttgccc ccggcgtacc agacggacat gggaaagaaa
     3361 tccttgtttt tttcgtcggc gaatccggtc gcggcggccg cggccaaaac cgcccccgcg
     3421 gccagaatca agagggggat tcttttagaa acgttcggca tgccggctct ccttgtcctc
     3481 cccccgccgg gggagattgg ttcgatggag gaaaaagtct ccgaccgcct cgagcaggac
     3541 ggattgtccg cgccagcggt cgtggctgag ctcccagatg atgaagccgg ccaccccgtt
     3601 gttcagggca taccggcact tgctccgaac cgagaggacg tcctcgtagg agacgatcgc
     3661 tcctccgccg gggctctgaa gcaaaggaac ccgggcgcaa acgtcccggg aggacgtcca
     3721 gccgttcgcc aggcggtttt ggacctcggt gaagctcact tccccgctcg tttgaaaggg
     3781 caggcccagg gccgggca
//
LOCUS       DAOOXH010000148         3768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_29037_length_3768_cov_7.079720, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000148 DAOOXH010000000
VERSION     DAOOXH010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29037_length_3768_cov_7.079720"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PKV64_11220"
     CDS             <1..740
                     /locus_tag="PKV64_11220"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPN17685.1"
                     /translation="FEGRADFCTAWISQTLTLDRPVVNICQVLQKSSLMLVAKSESGI
                     LQPEDMNGRTVAIWGGDFSIQPTAFFRKFNIKPVLVPQSYNIDAFAVGACDVTSAMYY
                     NEYNKIYLSGIDQEELVPFFFSDYGLNFPEDGVYCTRETYETRPDVCRKMRRAVLKGW
                     EYAAGHQEETLDVLMDYCASYHVRTNRAHQKWMLTVVLQAVVYGVGSDPAGWGVLRRD
                     DFRRVAEELKRQGFIQVVPAYEDFARE"
     gene            737..2668
                     /locus_tag="PKV64_11225"
     CDS             737..2668
                     /locus_tag="PKV64_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPN17686.1"
                     /translation="MTVMRRRSSAVKRPIVRRLIIWIGFPLLLVYAAVAVTNYVQSSR
                     AAVERMNSYLLELTNHHAAVLDSRFKAISQTPLIIAENIEAMRLLNEPRLYDLMERRL
                     AIDPGIFGMSISFEPYALDPGRERCSPRVFRSAGGLERAGAAGLVAYHTADWYLIPEL
                     LKSSYWTEPYYSAGGGNALMCTFSHPFSREGAFAGVVTADISLAELDKTMRGIEIISG
                     YTFLVGRSGTFISHPNPDFVMRESLFSLAEMLNRPDIRAIGKNMIRGRQGVIAFKDLD
                     TGEKSWMVYTPIPTCRWTFAAVIPEDRILAPVKAQLIRQVILMAMGLAGILAVIVWAA
                     YGITNPLRKLAAFAGRISSGDLTGRIEGIRGKDEIQELAGVLNQMVGDLNQYIRDFAA
                     ATKAKESVESELRIARQIQESLLPRVFPPFPDRTEFDLYAKNAPAKDVAGDFYDFFFL
                     DEDHLAVLIADVSGKGVPAALFMAVNRTLMKTVCRKGVGPAESLRAANEILSRDNDAC
                     MFTTMFLAVFNVRTGGLTYANAGHNPPMIVSRDRTLRTLESMGDVFLGVETGVSYHEG
                     RTRLEPGDLLMLYTDGVTEAAAPDGSFFGEERLAGFMAERRDDALSAIWGRLAADLDE
                     FQKGSQFDDITVVLLRRNE"
     gene            2707..3330
                     /locus_tag="PKV64_11230"
     CDS             2707..3330
                     /locus_tag="PKV64_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HPN17687.1"
                     /translation="MAAEFIKTLLSSFVAFIALVNPVQKIFVIHSLQRQFDGKTTRFI
                     SVKSTLTALIILVAFLAAGHVIFNYVFHIELYAFQMTCGIVLFYNGLTGLQRGVFFKM
                     DKNISAKDLTAVPIAIPMIAGPATITAAVTFPADYGNGVTVIAIVAALAVNLVFMLAA
                     KKIGDVLITFNLMNPLIRIFGLIVATIGLQMVLNGIHGFILPMTARG"
     gene            complement(3558..>3768)
                     /locus_tag="PKV64_11235"
     CDS             complement(3558..>3768)
                     /locus_tag="PKV64_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPN17688.1"
                     /translation="NGAYAIREHENRGSLEPGKFLDLVLCDMTTPADLVYHVGRNPVK
                     FVLKKGKVVVSGGRIVRGDRADSSP"
BASE COUNT          674 a         1202 c         1152 g          740 t
ORIGIN      
        1 tgttcgaagg gcgggccgac ttctgcaccg cctggatctc ccagacgctg accctggacc
       61 ggccggtggt caacatctgc caggtcctcc agaaatcctc gctcatgctc gtcgccaagt
      121 cggaaagcgg gatccttcag cccgaggaca tgaacggccg gaccgtggcc atctggggcg
      181 gagatttctc gatccagccg acggcgtttt tccgcaaatt caacatcaag ccggtcctcg
      241 tcccccaatc ctacaacatc gacgctttcg cggtcggagc ctgcgatgtg acctcggcga
      301 tgtattataa tgaatacaat aagatctatc tttccgggat cgaccaggag gagctggtcc
      361 ccttcttttt ttccgattac gggctgaatt ttcccgagga cggcgtctac tgcacccgcg
      421 agacgtatga aacccggccc gacgtctgcc ggaaaatgag gcgggccgtc ctgaagggct
      481 gggaatacgc cgccggacac caggaagaga ccctggacgt cctgatggat tactgcgcaa
      541 gctaccatgt ccggaccaac cgggcccacc agaaatggat gctgaccgtc gtcctgcagg
      601 ccgtcgtcta cggcgttgga tccgatccgg ccggttgggg cgtgctccgc cgggacgatt
      661 tccggcgcgt cgccgaggaa ttgaagcgcc agggattcat ccaggttgtt cccgcctacg
      721 aggacttcgc cagggaatga ccgtcatgcg ccgccgctcg tccgccgtga aacggccgat
      781 cgtccggcgg ctgatcatct ggatcggctt tccgctgctc ctcgtgtacg cggccgtggc
      841 cgtgaccaac tatgtccaga gctcgcgggc cgccgtcgaa cggatgaatt cctatctcct
      901 cgagttgacc aaccatcacg cggccgtcct ggattcccgt ttcaaggcca tctcccagac
      961 gccgctgatc atcgccgaaa atatcgaggc gatgcgcctc ctcaacgagc cccgcctcta
     1021 tgacctcatg gagcgccgcc tggcgatcga tcccggcatt ttcggcatga gcatctcctt
     1081 cgagccgtat gccctggatc cgggccggga acggtgctca ccgcgcgttt tccgctcggc
     1141 cggcggcttg gagcgggccg gggcggccgg cctcgtggcc tatcatacgg ccgattggta
     1201 cctgatcccc gagctcctga agagctccta ttggacggag ccctactaca gcgccggcgg
     1261 cgggaacgct ctgatgtgca cgttttccca tcctttttcc cgggaaggag cgttcgccgg
     1321 cgtcgtgacc gccgacatct ccctggccga gctcgataag acgatgcggg gcatcgagat
     1381 catctctgga tacacgttcc tcgtcggccg gagcggaacg ttcatctccc atcctaatcc
     1441 cgatttcgtc atgcgcgagt ccctgttcag cctggccgag atgctgaacc ggccggatat
     1501 ccgggccatt gggaaaaaca tgatccgcgg gcggcagggc gtcatcgcct tcaaggatct
     1561 cgacaccggc gaaaagagct ggatggtcta cacccccatc cccacctgcc ggtggacgtt
     1621 cgccgccgtg atccccgagg accggatcct tgcgccggtc aaggcccagt tgatccggca
     1681 ggtcatcctc atggcgatgg ggctggccgg catcctggcc gtcatcgtct gggccgcgta
     1741 cggcatcacc aacccgctgc ggaagctggc ggctttcgcc ggccggatct cctccggcga
     1801 cttaaccggc cgaatcgagg gcatccgggg gaaggacgag atccaagaat tggccggcgt
     1861 cttgaaccag atggtcggcg acctcaacca atacatcagg gacttcgcgg ccgcgaccaa
     1921 ggccaaggag tcggtcgaga gcgaattgcg catcgcccgc caaatccagg aatccctcct
     1981 gccccgggtt ttcccgccgt tccccgaccg gacggagttc gacctctacg ccaagaatgc
     2041 gccggccaag gacgtcgccg gggacttcta cgatttcttt ttcttggacg aggatcattt
     2101 ggccgtcctg atcgccgacg tctccggcaa gggcgtcccg gccgccctgt tcatggccgt
     2161 caatcggacg ctgatgaaga ccgtctgccg caagggagtc gggccggccg aatcgctccg
     2221 ggcggccaac gagattctca gccgggacaa cgacgcctgc atgttcacga cgatgttcct
     2281 ggccgtgttc aatgtccgga ccggcgggct gacctacgcc aacgccgggc ataatccgcc
     2341 gatgatcgtc tcgcgcgatc ggacgctccg gacccttgaa tccatgggcg acgtcttcct
     2401 gggcgtcgag acgggggtgt cctaccacga aggccggact cgtctcgagc ccggggacct
     2461 cctgatgctc tataccgacg gagtgaccga agcggccgcg ccggacggga gcttctttgg
     2521 agaggagagg ctcgccggtt tcatggccga gcgccgggac gacgccctgt cggcgatctg
     2581 gggccggctt gcggccgacc tggacgaatt tcagaaaggc agccaattcg acgacatcac
     2641 cgtcgttctg ctcaggcgga acgaatagcc gggccgccgg tccggggaga gaggggaaac
     2701 aagatcatgg ccgcggaatt catcaaaacg cttctcagca gcttcgtggc tttcatcgcc
     2761 ctggtcaacc cggttcagaa gatcttcgtg atccattcgc tccagcggca attcgacggc
     2821 aaaaccaccc ggttcatctc ggttaagtcg accctcaccg ccctgatcat cctggtggct
     2881 ttcctcgcgg ccggccatgt cattttcaac tatgttttcc atatcgagtt gtacgcgttc
     2941 cagatgacct gcggcatcgt tctgttttac aacggcctta ccggcttgca gcggggcgtc
     3001 ttcttcaaaa tggacaagaa catctcggcg aaggatctga ctgccgtgcc catcgccatc
     3061 ccgatgatcg ccggacccgc gaccatcacc gccgcggtga cattcccggc cgattatggg
     3121 aatggagtga ccgtgatcgc gatcgtcgct gccttggccg tcaacctcgt tttcatgctg
     3181 gcggcgaaaa aaatcgggga tgttctcatc acgttcaacc tgatgaatcc gctgatccgc
     3241 attttcggat tgattgtcgc gacgatcggc ctccagatgg tcctgaacgg aatccacggc
     3301 tttatcctcc cgatgacggc gagggggtag cggagggcgt tcttttcaac gcgcctcggg
     3361 ctgacgcggg ccgcgttctt ccagacggag acaggtcttt aatccgatcc ctcagcgggc
     3421 gcggtttatt cggacgcgga agagcgaacc aaggccggga gggctcgtcc ctccgcgggg
     3481 cgggtttttc aggagcgggg ggaatcgggg gcgcctcctt gcggagacgc ccccttggga
     3541 caaaaaggga ttgggaccta gggggaggag tcggcccggt cgccgcggac gatccggccg
     3601 ccggagacga cgaccttgcc tttcttgagg acgaacttca ccggattgcg tccgacgtgg
     3661 tagaccaggt cggcgggcgt cgtcatgtcg cagaggacca ggtccaggaa ttttccgggc
     3721 tcgaggcttc cgcggttctc gtgctcccgg atggcgtaag cgccgttg
//
LOCUS       DAOOXH010000149         3757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_29134_length_3757_cov_6.612642, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000149 DAOOXH010000000
VERSION     DAOOXH010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29134_length_3757_cov_6.612642"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..1500)
                     /gene="lysA"
                     /locus_tag="PKV64_11240"
     CDS             complement(256..1500)
                     /gene="lysA"
                     /locus_tag="PKV64_11240"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPN17689.1"
                     /translation="MFWWENEFLQVKAGRLFPAGRDAAAIAGGHGTPLFIYGSERIRA
                     NFRRLASGAGPSPGREIRICYALKANAHPGILSLLKNEGAWIDAVSPGEVQAAVRAGF
                     PPGRILFTGTSCGTSDFEPLLRRPGLTINLDALEQFPLLKAARDRIAPGRRVRISVRW
                     NPGLGRGFNPKVITAGARSANGTPVKFGIEPKDVPGLFRRARAAGFDPVGIHQHLGSG
                     WVRDDYPAVLEAVDRIVGLARRLEDEGFPLEFVDFGGGFGARYARSQKPFPVERYLRT
                     LLRRLDEAGIKAPALAVEPGKYLVADAGLVLLRVEYLKRGFGNLFACVNAGTFNTVPR
                     PAIYDTARHEIINATRVDEKPKRKMTIAGHLCETGDVFGIDVRLPEPRPGDLLAVLNA
                     GAYARSMASTFNQRPIPEEILV"
     gene            complement(1505..>1896)
                     /locus_tag="PKV64_11245"
     CDS             complement(1505..>1896)
                     /locus_tag="PKV64_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HPN17690.1"
                     /translation="PTAVVTDPRFAYVRLHGRNAKDWFREGAGRDARYNYLYAKNELE
                     EWIGRIKELGRSTDRIYVITNNHYRGQALANALQIKNLTSGKKLEVPEELLRQYPVLE
                     EIIERLRKGQFDLFDDDPGRTSGGTKG"
     assembly_gap    1897..1996
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1997..2271)
                     /locus_tag="PKV64_11250"
     CDS             complement(<1997..2271)
                     /locus_tag="PKV64_11250"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HPN17691.1"
                     /translation="MPQVLVGTAGWSYEDWKGVVYPEGLGRLHPLSYLARYVDCVEVN
                     STFYRTPPVPMVQGWLKAVASRPGFQFAVKVHQAFTHERKDFSAGDV"
     gene            2397..3383
                     /locus_tag="PKV64_11255"
     CDS             2397..3383
                     /locus_tag="PKV64_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPN17692.1"
                     /translation="MVTASDIRAAYGRIRGDIVRTPLEYSPALSRLTGARVFVKWESD
                     QITGSFKFRGALNKMRTLPADRKKAGVIAASTGNHGLGVARAAGLEGTAAVLYLPKTA
                     VPVKAARLKEAGAVVERRGASCEKAEAAARRAAGRAGMVYISPYNDPEVIAGQGTIGL
                     EILADCPEAEDVFVPVGGGGLIAGLAIFVKSVKPEIRVIGVEPAASAFMNASLRAGRL
                     VSVREGRTAADAVAGGIEPGSITFPLCRAWVDEIRTTGEAFLKEALRLCFRHHGRIVE
                     GAGALALAALLKAGRSLRNRTVVLAASGGNVDPARWREITGLDPGKRGGRRR"
     gene            complement(3348..>3757)
                     /locus_tag="PKV64_11260"
     CDS             complement(3348..>3757)
                     /locus_tag="PKV64_11260"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HPN17693.1"
                     /translation="RGNASVLTPNLAEAARLAERPVRTISEMGRAAAVIFDRTGVPCL
                     VKGGHLPDHPVNLLFDGRREHRFPQKRIAAGLHGTGCLFSAALLCCLVRTGDLAAAAE
                     AATAWTHRAIRAAARTGRWPAALPPPPAALSRI"
BASE COUNT          594 a         1173 c         1305 g          585 t
ORIGIN      
        1 gtgttcctga aggacgtccg ggccgtcccg gccgtcgtcc tggctgtcgt gacggccgcc
       61 gccctggccg cggcccgcct ggatgagcgg gagatgacgg cccggttcgg ggcggcctac
      121 gccgactaca tgaaaaggac gaaaaggttc atcccgttcc tctggtgaac gggaggcggg
      181 ccggacaaag acccccggcc gcggcggaca gccggaaaac gcccgcctgg gtttcggccg
      241 gcggccgaga gaatctcaga cgaggatttc ctcggggatc ggtctctgat tgaatgtgga
      301 ggccatggac cgggcgtacg cgccggcgtt caggacggcc agaagatcgc ccggcctcgg
      361 ctcggggagc cggacgtcga tcccgaagac gtcgccggtc tcgcagagat gtccggcgat
      421 cgtcatcttc cgcttcggct tttcgtcgac ccgcgtcgcg ttgatgatct cgtggcgggc
      481 ggtgtcgtaa atggccggcc ggggcacggt gttgaacgtc ccggcgttga cgcaggcgaa
      541 caggttgccg aagccgcgct tgagatattc cacccggagg aggaccagcc cggcgtcggc
      601 gacgagatat ttcccgggtt cgacggccaa ggccggggct ttgattccgg cttcgtcaag
      661 ccgccggagg agggtccgga ggtagcgctc gacggggaag ggcttctggc ttcgggcgta
      721 gcgggccccg aagccgccgc cgaaatccac gaattccagg ggaaagccct cgtcttccag
      781 ccttcgggcc aggccgacga tccggtcgac ggcttcaagg acggcgggat aatcgtcccg
      841 gacccagccg gagcccaggt gctgatggat tccgaccgga tcgaatccgg cggcccgggc
      901 ccggcggaag aggccgggca catccttggg ttcgatcccg aacttgaccg gcgtgccgtt
      961 ggccgaccgg gcgccggcgg tgatgacctt gggattgaac ccgcggccga ggcctggatt
     1021 ccagcggacg gaaatccgga cgcggcgccc gggggcgatc cggtcgcggg cggctttcag
     1081 gagcgggaac tgctcgaggg cgtccaggtt gatggtcagg ccggggcggc ggaggagcgg
     1141 ctcgaaatcc gaggttccgc agctcgtccc ggtgaaaagg atccgccccg gaggaaatcc
     1201 cgcccggacg gccgcctgga cctccccggg cgacacggcg tcgatccagg cgccttcgtt
     1261 tttcaggagg gacaagattc ccggatgggc gttggctttc agcgcgtagc agatgcggat
     1321 ctcccggccg ggcgacggac cggcgccgga cgccagccgg cggaaattcg cccggatccg
     1381 ctcggacccg tagatgaaga gaggggttcc gtgcccgccg gcgatcgcgg cggcgtcgcg
     1441 gccggcgggg aacaagcgcc ccgccttcac ttggagaaac tcgttttccc accagaacat
     1501 gggatcagcc tttcgttccg ccggacgtcc gtccgggatc gtcgtcgaaa agatcgaatt
     1561 ggcccttgcg cagtcgctcg atgatctcct ccaggaccgg gtactgtctc agcagctctt
     1621 ccgggacctc gagctttttc ccgctggtca ggttcttgat ctggagggcg ttggccaggg
     1681 cctggccccg gtaatggttg ttcgtgatga cgtagatccg gtccgtcgag cggcccaatt
     1741 ccttgatccg gccgatccat tcctccagct cgttcttggc gtataagtaa ttgtaccggg
     1801 cgtcgcggcc ggcgccctcc cggaaccaat ccttggcgtt gcggccgtga agccggacgt
     1861 aggcgaaccg cggatcggtc accacggccg tcggccnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnntcga cgtccccggc cgagaaatcc ttccgctcat gggtgaaggc
     2041 ctggtggacc ttgaccgcga attgaaaacc agggcgggag gcgacggcct tgagccatcc
     2101 ctggaccatc gggaccggcg gcgtccggta gaaggtgctg ttgacctcga cgcaatcgac
     2161 gtagcgggcc aggtaggaaa gcggatgaag gcggcccagc ccctccggat agaccacgcc
     2221 cttccaatct tcgtagctcc agccggcggt tccgacaaga acttgaggca tcgtttcggc
     2281 ttctatttta cggaatcacg ggccggactc aagacgcggc cgtttccttc ccggtttgaa
     2341 ccggggggcg gaacggatcg cttgatccgc ggccggaatt gtggaatata gatgccatgg
     2401 tcacggcaag cgatatccgg gcggcttacg gccgcatccg cggcgacatc gtccgcaccc
     2461 cgctggaata ttcgccggcc ctgtcgcggc tgaccggcgc ccgcgttttc gtcaaatggg
     2521 aatccgatca gatcacgggc tcgttcaagt tccggggggc cttgaataaa atgcggacgc
     2581 tcccggcgga ccggaagaaa gccggcgtca tcgccgcctc gacggggaac cacgggttgg
     2641 gcgtcgcccg ggcggccgga ctggagggaa cggccgccgt gctttacctt ccgaagacgg
     2701 ccgtcccggt gaaagccgcc cggctgaagg aagcgggagc cgtcgtcgag cggcgcggag
     2761 ccagctgcga aaaagccgag gcggccgccc ggcgggccgc cggccgggcg ggaatggtct
     2821 atatctcgcc ttacaacgac cccgaggtca tcgccgggca gggcacgatc ggcctggaga
     2881 tcctcgccga ctgccccgag gccgaggacg tctttgtgcc cgtcggcggg gggggattga
     2941 tcgccggcct cgcgatcttc gtgaagagcg tgaagccgga gatccgggtt atcggcgtcg
     3001 agccggcggc ctccgctttc atgaacgcgt cattgcgggc cggacgcctg gtgtccgtcc
     3061 gggaaggccg gacggccgcc gacgccgtcg ccgggggcat cgagcccggc tcgatcacgt
     3121 tccccctctg ccgggcctgg gtcgacgaga tccggacgac gggggaagcg ttcttgaaag
     3181 aagccctgcg gctctgcttc cggcatcacg gccggatcgt cgagggggcc ggggccctgg
     3241 ccctggccgc tcttctcaag gccgggcggt ccctgcggaa ccggacggtc gtcctggcgg
     3301 cgagcggcgg gaacgtcgat ccggcccgat ggcgcgaaat caccggctta gatccgggaa
     3361 agcgcggcgg gcggaggagg tagggccgcc ggccaacgcc cggtccgggc ggccgcccga
     3421 atcgcccgat gcgtccaggc ggtggccgct tccgcggccg cggccaaatc acccgttcgg
     3481 acaagacagc aaagcagggc cgcggaaaaa aggcagcccg tcccgtgaag gccggccgcg
     3541 attcgttttt gcgggaatcg atgctcccgg cgtccgtcga acaggaggtt gacgggatgg
     3601 tccggaaggt gtccgccctt gaccagacaa gggacgccgg tccggtcgaa aatcacggcc
     3661 gccgcccgcc ccatttcgga gatcgtccgg acaggccgct ccgccagccg ggcggcctcg
     3721 gcgagattcg gagtcagaac cgaggcgttt ccgcgaa
//
LOCUS       DAOOXH010000150         3734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_29420_length_3734_cov_4.430280, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000150 DAOOXH010000000
VERSION     DAOOXH010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29420_length_3734_cov_4.430280"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /gene="asnB"
                     /locus_tag="PKV64_11265"
     CDS             complement(<1..1524)
                     /gene="asnB"
                     /locus_tag="PKV64_11265"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HPN17694.1"
                     /translation="MSGIAGLLHLDGAEPSPSILERMIRTLVHRGPDGEGIRIAGPVG
                     LGHRRRTAAAGEEAGGQPLSNEDRSVWITADAAIYNDREIRGELRERGHVFRSQADAE
                     IIIHAYEEWGPECLRKFNGVFAFGLWDDRLRRLWLVRDRLGVKPLFYARLPNAFLFGS
                     EIKALLAHPAVKRNVDVPALAYFLGLNYTPAPRTLFREIRQVLPGHYLLAGASGVPEE
                     CEYWDLRFQEGGDSRREDDYLEEFEALLADAVRLRLRGDIQPGAFLSGGIDSSALVYW
                     MRRLGRGPLRTFTVGFPERSFDELSYARSTAGRLGTEHIERIIGAEAAAILPEIVRHA
                     EEPTADSSMVAVYFLAQTAGRLVPAVLSGEGADEILAGYETYQAYYARRAYRILPAFL
                     RRGILRLACAAWPVSERKESVESRLRRFAAGAETEGEAAHGAWRMIFTPDERRDLLRP
                     TADPAAGADYLELYRRYFQQAGARHPLNRMLYVDTRFYLPNDMLVKADRMCMAHGLEA
                     "
     gene            complement(1528..2127)
                     /locus_tag="PKV64_11270"
     CDS             complement(1528..2127)
                     /locus_tag="PKV64_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17695.1"
                     /translation="MDADGQHRAAEARKLWEAAGPNDMVVGKRLRLIHSPLWRMPGKW
                     LLSWMAGYLVRRRIPDLNSGLRLIKRPLAARYMSLCPKGFSFSTTITLILIQQGCEVA
                     YVPIEVGKRRGKSRVSPATGFETLLLVLRLATLINPLRLFLPLSAGLGLIGVLWGIPY
                     FLAGHGISIGSLLAIVTALLLFAIGLISDQISQMRLEKL"
     gene            complement(2031..2378)
                     /locus_tag="PKV64_11275"
     CDS             complement(2031..2378)
                     /locus_tag="PKV64_11275"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPN17696.1"
                     /translation="MPSLSFIIPAYNEAESIAAVVRDIQAHFAAEEIEIIAVDDGSTD
                     QTAAEAENAGSAVVRHPRNLGYGASLKSGISRARGDWVLLWTPTGSTGPRKRASSGRR
                     PVRTTWSSGNAFG"
     gene            complement(2379..>3734)
                     /locus_tag="PKV64_11280"
     CDS             complement(2379..>3734)
                     /locus_tag="PKV64_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17697.1"
                     /translation="FQNKAIALFFTLAAAVPIKISLVEWGFMRDVVPRFLFQALLPFV
                     LAAFIKRGRNPKSWPWIMGGVGALVYVHPVSFPAWGSALALSLWVYTPEIPARSKIRP
                     WLGAVFMMILIAAPFAVHYLAATDFGSSSGGNQEAVRMIMEKRFAAGYMDIGSGLRKI
                     AGDAVFSHGLNLGLWILAAAGGIALALRRNRIRSPLPRVMAAWWAAIIAVGVLIPAAD
                     HVLSTSLNRTPLEIDLIRSLRYLFPLLLLTAFFLFAAMRTAAADIKRARLRPWLRAGA
                     VLLAAGVMLAWMARCRLIENPEFRQTARCLESGRIIGPFPDEDRLDRRIGLLQALRSL
                     TPPGSRILCSRVEDLAVRYFSLRPLAFAHKDGGALSYANHEELLTWWRRWQGFARVRR
                     IKDRGLYLEALAAYAREIRADYLIVPEAFDSATRYPDGWLNIYSNPDSSIFKTAGGED
                     R"
BASE COUNT          761 a         1193 c         1192 g          588 t
ORIGIN      
        1 cgcttccaag ccatgggcca tgcacatgcg gtccgccttg accagcatat cgttgggcag
       61 gtaaaaacgg gtgtcgacat agagcatgcg attcaaggga tgccgggcgc cggcctgttg
      121 aaaatagcgg cgataaagct ccaaataatc ggccccggcg gcgggatcgg ccgtcggccg
      181 gagcagatcc cgtctttcgt ccggggtgaa aatcatccgc cacgcgccgt gggcggcctc
      241 gccctccgtc tcggcgccgg cggcaaagcg cctcagccgg gattcgacgc tttctttcct
      301 ttccgacacc ggccaggcgg cgcaggcgag ccgcagaatc ccccggcgca gaaaagcggg
      361 aaggatccgg tatgcccggc gcgcataata agcttggtag gtttcataac cggccaggat
      421 ttcatccgcc ccttcgccgc tcaggacggc ggggacaagg cggccggccg tctgggccag
      481 aaagtacacg gccaccatgg aggaatcggc cgtcggctct tccgcgtgcc ggacgatttc
      541 gggaagaatc gccgccgcct cggccccgat gatccgttcg atgtgttcgg tcccgagccg
      601 gccggccgtc gaccgggcat aagacaattc gtcgaagctc cgctcgggga acccgacggt
      661 gaacgtccga agaggtccgc ggccgaggcg ccgcatccag tagaccagcg cgctcgaatc
      721 gatcccgccg cttaaaaacg cgcccggctg gatgtccccc ctcaggcgca ggcggacggc
      781 gtccgcgagc agggcctcga attcctccag ataatcgtct tcccttcggg aatcgccccc
      841 ttcctgaaag cgaaggtccc agtattcgca ctcctccgga acgcccgagg cgccggccag
      901 caggtaatgg ccgggcagaa cctggcggat ctcccggaaa agcgtccgcg gcgcgggggt
      961 gtaattcaga ccgagaaaat aggccagcgc cgggacatcg acgttccgct tgacggccgg
     1021 atgggccagg agggctttga tttccgaccc gaacaggaac gcgttcggca agcgggcgta
     1081 gaagaggggc ttgacgccga gccggtcccg gaccagccag agccgccgga gacgatcgtc
     1141 ccacaagccg aaagcaaaaa cgccgttgaa tttccgcaag cactccgggc cccattcctc
     1201 ataggcatgg atgatgattt cggcgtccgc ttgggaacga aagacatgcc cccgctcccg
     1261 caattcgccg cggatttccc ggtcgttgta gatcgcggcg tcggcggtga tccacacgga
     1321 ccggtcttca ttgctcaagg gctgcccccc ggcttcttcg ccggcggcgg ccgtccgccg
     1381 ccggtggccc aggccgacgg ggccggcgat ccggattcct tccccgtccg gcccgcggtg
     1441 gaccagcgtc cggatcatcc gttcgagaat cgacggcgac ggctccgccc cgtccagatg
     1501 gaggaggccg gcgattccgc tcatctctta caacttctcc agccgcatct gtgaaatctg
     1561 atcgctgatc aagccgatgg cgaagaggag cagggccgtt acgatcgcca gcagggaacc
     1621 gatgctgatc ccatggccgg ccaagaaata aggaatgccc cataaaacgc cgataagccc
     1681 gaggcccgcg ctgagcggca ggaagaggcg cagggggttg atcaaagtgg cgagcctcaa
     1741 aacgagcaaa agagtttcga atccggtggc cggggacacc cggctttttc cccgccgctt
     1801 cccgacctcg atcggcacgt aagcgacttc gcagccttgt tggatgagaa tcagggtgat
     1861 ggtcgtggaa aaggaaaagc ccttcgggca caggctcatg taacgggcgg cgagcggacg
     1921 cttgatgagc cgcaggcccg aattcagatc ggggatgcgc cggcgaacca gatagcccgc
     1981 catccaggac aacagccatt tccccggcat gcgccataac gggctgtgga tcagccgaag
     2041 gcgtttcccg acgaccatgt cgttcggacc ggccgcctcc cagagcttgc gcgcttccgc
     2101 ggcccggtgc tgcccgtcgg cgtccataac agcacccagt ccccgcgggc gcggctgatg
     2161 ccggatttca aggaggcccc gtaacccaag tttcgagggt gccggacgac cgcggacccg
     2221 gcgttttcgg cctcggccgc agtctgatcc gtcgagccgt catcgacggc gatgatctcg
     2281 atttcctccg cggcgaaatg ggcctggata tcgcggacca cggcggcgat ggactcggcc
     2341 tcgttatagg cgggaataat gaaggacagc gacggcattt aacgatcctc cccgccggcg
     2401 gttttgaata tcgaagaatc cggattggaa tagatgttga gccatccgtc cggatagcgg
     2461 gtcgccgaat cgaacgcctc cggcacgatc aaataatccg cccggatttc ccgggcatat
     2521 gccgccaagg cctcgaggta caaaccgcga tccttgatcc ttctcacgcg ggcgaatcct
     2581 tgccatcggc gccaccaggt caaaagctcc tcatgattgg cgtagctcaa ggccccgccg
     2641 tccttatggg cgaacgccag cgggcgaagc gaaaaatatc gaacggcgag atcctcaact
     2701 cgtgagcaca ggatgcgcga gccaggggga gtgagggagc ggagagcctg gagcaggccg
     2761 atccggcggt ccaggcggtc ctcatcgggg aaagggccga tgatccggcc ggactccagg
     2821 cagcgcgcgg tctgccggaa ctcggggttt tcgatgagcc ggcagcgggc catccaggcc
     2881 agcatcactc cggcggccag caaaacggct cccgccctga gccaaggccg gagacgcgcg
     2941 cgcttgatgt ccgccgcggc cgttctcatc gcggcgaaca ggaaaaacgc cgtgagcagc
     3001 aacaacggaa agagatatct caggctccgg atcagatcga tctctaaagg cgttctgttg
     3061 aggctggtcg agaggacatg gtccgccgcc ggaatcaaga cgccgacggc gatgatcgcc
     3121 gcccaccaag cggccatcac ccggggaagg gggctccgga tccggttccg gcgaagggcc
     3181 agggcgattc ctccggcggc ggccaggatc cacaatccca gattcagccc gtgggaaaaa
     3241 accgcgtccc cggcgatttt cctcaatccg gatccgatgt ccatgtaacc ggcggcgaag
     3301 cgcttttcca tgatcatgcg gaccgcttcc tggttccctc cgctcgaaga gccgaaatcc
     3361 gtggcggcca gataatgaac ggcgaacggg gcggcgatca agatcatcat gaacacggcc
     3421 ccgagccagg gccggatctt cgatcgcgcc gggatttccg gagtgtagac ccagaggctc
     3481 aaggcgagcg ccgatcccca ggccggaaag ctgaccggat ggacatagac gagcgcgccc
     3541 actccgccca tgatccaggg ccaggatttc ggattccggc cccgcttgat gaaggcggcc
     3601 aaaacaaaag gcagaagcgc ttggaagaga aagcggggaa cgacatcgcg catgaatccc
     3661 cactcgacca ggctgatctt gatcgggacc gcggcggcca aggtgaaaaa caaggcgatc
     3721 gccttgtttt ggaa
//
LOCUS       DAOOXH010000151         3723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_29545_length_3723_cov_6.747001, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000151 DAOOXH010000000
VERSION     DAOOXH010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29545_length_3723_cov_6.747001"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PKV64_11285"
     CDS             complement(<1..268)
                     /locus_tag="PKV64_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17698.1"
                     /translation="MESLSAKELVELVRSVFPHHPGDSILSILTDVPRTASSDHPAWK
                     SRRETAFRWMGPLYDARQDIPMDDVRLYVYPDVGSNNADLPADAY"
     gene            complement(329..2293)
                     /locus_tag="PKV64_11290"
     CDS             complement(329..2293)
                     /locus_tag="PKV64_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN17699.1"
                     /translation="MTLYHHSRRVCLLIPAIAVLIGQAAASKDASPETTPSFLSHEIV
                     VTAARLETPLIEIASAVTVITREDLERTRPASVLDALRDTPGLGLVRNGGPGAASSIF
                     VRGANSEHVLVLLDGVPLNDPINPSRSFDLAHMTLDGVERIEILRGPQSTLYGSDALG
                     GVINILTGRGMGKPAVTFRARGGSHGTLSGGLDIRGSAGAFRYDLSASRESTEGVSAA
                     SSAYAGNTEQDGYRNWTLSGRAGLVLAGGTEIDLIIRGVSARTDLDAFGGAYGDDPNS
                     IQDYRSGFVRAQIRTLSAGGRWESKAGFAYIRSTRENDNPVDESHPFDSETARYRSAR
                     FRADWQNNLFLRPGHTLTAGAEIERETGDSAYFSESLWGPYASVFSRRHADRAGVFVQ
                     DRFRLGRAFFATAGLRLDLHGRSGTSLTFRLAPAYLIEKTGTKLKATIGTAFKAPSLY
                     QLYAPETLWGPVGNEHLLPEESLGWDAGVEQTLAGGAILLGASFFQSDFRNLIDYDFL
                     QGYINIGRARTSGWEFTADARPVERLTLRLGYTRLAAEDLDAGVSLLRRPKDKLSGAI
                     EWTPATGWKAVLTAAATGERRDYDYTGWAAVPVILPPYAIVDALLSYTPAPGLEFFGR
                     WDNIGNTRYETVFGYGTWGSSISAGCTVRF"
     regulatory      complement(2322..2514)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2688..3377
                     /locus_tag="PKV64_11295"
     CDS             2688..3377
                     /locus_tag="PKV64_11295"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPN17700.1"
                     /translation="MKTPCLIDALFGKTKRSILVLLFGHDDEAFHLRKILRLANISPG
                     AGQRELKKLADAGLITRSVKDNQVLFQGNPHCPIFPEIKMLVTKTAGAADVLRAALDP
                     LIKRISVVLIYGSLARGSAGNESDLDLLIVGEVRFEEIVNALHPVQDRLRREINPLVM
                     SSREFRRRLSQGDHLIREIMKSPFIPILGDPHDFGRMEKERMARRTLEPPPRDQSSLQ
                     AYRAGSEGLSK"
BASE COUNT          755 a         1071 c         1155 g          742 t
ORIGIN      
        1 gataggcgtc ggccggcagg tcggcgttgt tcgatccgac atcgggatag acataaagac
       61 ggacatcgtc catggggatg tcctgccggg cgtcgtaaag cggtcccatc cagcggaagg
      121 ccgtctcccg acggcttttc caggccggat ggtcggagga cgcggtccgg gggacgtcgg
      181 tgaggatcga gaggatgctg tctcccggat gatgagggaa aacggaccgg accagctcca
      241 cgagttcttt cgcgcttaaa gattccatct ccccctcccg ggttcattat acacgagttg
      301 agggaaaacg cccgcttccc tcgatccttt aaaaacgaac cgtgcacccg gccgaaatgg
      361 acgagcccca cgttccgtag ccgaagacgg tttcatagcg tgtgtttccg atgttatccc
      421 aacggccgaa aaattcaagc cccggggcgg gcgtatacga gaggagggcg tcgacgatcg
      481 cgtagggggg aaggatgacg ggcacggcgg cccagcccgt gtagtcgtaa tcccggcgct
      541 cccccgtggc cgcggccgtg agaacggctt tccatcccgt cgccggcgtc cactcgatcg
      601 ctcccgagag cttgtccttg ggccggcgga gaagggagac gccggcgtcg aggtcttcgg
      661 ccgccaggcg cgtgtatcca agccggagcg tcagtctctc gaccgggcgg gcatcggccg
      721 tgaattccca gccgctggtc cgggcccggc cgatgttgat gtagccttgg aggaaatcgt
      781 aatcgatgag gttccggaaa tccgattgaa agaaggacgc tccgaggagg atcgctcctc
      841 ccgcgagcgt ctgctccacg cccgcgtccc accccaacga ttcttcggga agcagatgct
      901 cattgccgac gggaccccag agcgtctccg gggcgtacaa ttggtagagc gaaggcgcct
      961 tgaaggcggt tccgatcgtg gccttcagct tcgtccccgt tttttcgatg aggtaggcgg
     1021 gggccaggcg gaaggtcagc gaggtcccgc tccggccgtg aaggtcgagc cggaggccgg
     1081 ccgtggcgaa aaacgcccgg cccaggcgga acctgtcctg gacgaacact ccggcccggt
     1141 ccgcatgacg gcgggagaag acgctggcgt acggccccca aaggctttcc gagaaatagg
     1201 ccgagtcgcc cgtctcccgc tcgatctcgg ctccggccgt cagcgtgtgg cccggccgga
     1261 ggaagaggtt gttctgccag tccgccctga accgggcgct gcggtaacgg gcggtctccg
     1321 agtcgaaggg atggctttcg tcgaccgggt tgtcgttttc gcgggttgaa cggatgtagg
     1381 cgaagccggc tttggattcc caacgccctc cggcgctcag ggtccggatt tgggcccgga
     1441 cgaacccgga ccggtaatcc tggatgctgt tgggatcgtc cccgtaggca ccgccgaagg
     1501 cgtcgagatc ggtccgggcc gaaacgcccc ggatgatcag gtcgatctcg gtgccgccgg
     1561 ccaagaccag gccggcccgg ccggacagcg tccagttgcg atagccgtct tgttcggtgt
     1621 ttccggcgta ggcgctggag gcggccgaaa cgccttcggt cgattcgcgc gacgcgctga
     1681 ggtcataccg gaacgccccg gccgaccccc ggatgtccag tcctccggag agggttccgt
     1741 gcgacccccc gcgggcgcgg aacgtcaccg ccggtttccc catcccccgg ccggtcagga
     1801 tgttgatgac gcctccgagg gcgtccgaac cgtagagggt gctttgggga ccgcggagga
     1861 tttcgatccg ttcgacgccg tcgagggtca tgtgggcgag gtcgaaggag cgggacgggt
     1921 tgatcggatc gttcaggggc acgccgtcca ggaggacgag aacatgttcc gaattcgccc
     1981 cccggacgaa aatggacgaa gccgctcccg gcccgccgtt gcggacgagg cccaagccgg
     2041 gcgtgtcgcg gagagcgtcg aggacggagg ctgggcgggt ccgttcgaga tcctcccggg
     2101 tgatgaccgt gacggcgctg gcgatctcga tcaggggcgt ttcaaggcgg gcggccgtga
     2161 cgacgatctc gtggctgaga aacgaaggcg tcgtctccgg cgaggcgtct ttggaagccg
     2221 cggcttgtcc gataagaacc gcgatcgccg ggattaagag acagacacga cgggaatggt
     2281 gataaagggt catgagaaac ctcctcttcc gacccgaaga ttggtttcgc tcaagccggt
     2341 gaaggtttcc tggctctcgg atcatcctcc tcccgggcct tcccgccgcc ttgcggcgac
     2401 cgtggcgtct tccgggattc gtacccgatt acagtagcgg ggactgcgtc cgcttttcac
     2461 ggacttccct tcgctccagc tcgcgacgag agtccattct cttcttttcg atttttattg
     2521 tcaagattat gtacggtgct aaatgcggcc tcagaatcat ttaggcgtat gagaattatc
     2581 tgcggaaatc gcgtcgtata ggttggtgat tgatacttgc aatatgtaac caaaaaaggt
     2641 acaatcgtac ccgaaatgag tacaaatgta cccataatgg atacataatg aaaacaccct
     2701 gtctgatcga cgcgctcttc gggaaaacga aacggtcaat tctggtcttg ctcttcggcc
     2761 acgacgatga agcatttcac ttaagaaaaa ttctgcggct ggcgaacatt tctcctggag
     2821 caggacagag agaactgaaa aagctggcgg atgcggggct gatcacccgc tccgtcaagg
     2881 acaaccaggt tctctttcag ggcaatccgc actgcccgat tttcccggaa ataaaaatgc
     2941 tcgtgaccaa gacggcgggc gcggccgatg ttctgagggc ggctcttgac cctctaatta
     3001 aaaggatctc cgttgtgctc atttacggct ctctcgctcg gggctcggcc ggcaacgaaa
     3061 gcgatcttga tctccttatc gtcggtgaag tgaggttcga ggaaatcgtc aacgctctcc
     3121 acccggttca ggaccgtctc aggcgcgaaa tcaatcccct ggttatgtcg tccagggaat
     3181 tccgcagaag gctctcccaa ggcgatcatc tgatccgcga aattatgaaa tcgccgttta
     3241 ttccgatact cggagatccg catgactttg gccgaatgga aaaagaacga atggctcgtc
     3301 gaacacttga gccgccgcca agagatcaat catctcttca ggcttatcga gcgggatctg
     3361 aaggactgtc gaaataagaa gctcagtctc gattggcgtt tcaatatcgc ttataacgct
     3421 gcccttcaat gcgcgaacgc cgctctggca gccgagggct tccgagccgc caaggattcc
     3481 catcatttcc gagtcatcca atccttgaaa ttcacgctcg aaattaatga caaaacgatt
     3541 cgtaaattcg atgtcttccg gaagaaacgg aatatcagcg agtatgaccg ggccgggtcg
     3601 gtatccattg cggaatttga tgaaatgatc gcgttagcgg aatttctcta caaatccgca
     3661 tcctcttgga tgaaaaagaa tcatgcggac cttatttgac gactgttctg atatacgaac
     3721 aaa
//
LOCUS       DAOOXH010000152         3672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_30058_length_3672_cov_6.134089, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000152 DAOOXH010000000
VERSION     DAOOXH010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30058_length_3672_cov_6.134089"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..745)
                     /locus_tag="PKV64_11300"
     CDS             complement(125..745)
                     /locus_tag="PKV64_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17701.1"
                     /translation="MKKAAIIVICIVVTSCAFTDITSITNPDHERKAYGRILIISPFQ
                     ELRYRKMFEYKLSNTFYDAHVDSFAGIDLISPIKSYSDYELRQVLDRYQIDGILVASL
                     TDYYESQTYVPPTQITSGSVWVVSNYVRYSQTTNAIGGGIISRPRMKFEIRLYDRSSG
                     EVAWLSQTFSKGNAFADFVTLADSLSRVTIRTLIKERRLLSSMTRK"
     gene            980..1357
                     /locus_tag="PKV64_11305"
     CDS             980..1357
                     /locus_tag="PKV64_11305"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HPN17702.1"
                     /translation="MKRPVFIAAAAVFVTILGLASVGCKPDPVPKIVLIEGLTLDVSV
                     SMFRVTGRIQNQGGGKAMYTRVKIYIRDAAGNILAYNWNYCDDTTLEAGETAGFEVLF
                     WDEDGKLRDKMDFSKSTYEFAYD"
     gene            1764..>3672
                     /locus_tag="PKV64_11310"
     CDS             1764..>3672
                     /locus_tag="PKV64_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17703.1"
                     /translation="MPRSWREGPFPAAWSTSEFVKWGYFAVALHVQVLTSLFYPEISE
                     RMSASIPGFVVEFGGSVLLGVAFAYFMLGGHHALHVLEAARTYNLERTLQATIGARLV
                     EKKADGTVRFRTDTAAYYKELARMFLLGPSGRHPAVIKDGLNYRLNGTPALNVAAAGS
                     RFDLAMAYVAGLSGLALFGLAVAARIDGRETGGALAFITPRILALGIIAFLGRHQDNN
                     ALYRYREKKKIEADLAARAAAGTLKADAGREAVDARAKSNYERLVGEVRNSAVYGGSD
                     PRFKGIAVYLPEGAHNAIQAGKHTEYVDAEGNKGNTSPDLVGQEKWIILFPRETEEPA
                     RRGFFGTLRVLPSLQSQIVAEILAREGVSGGGRENEGGCSFNGYGGGDEAAIDLLMAA
                     AAKAGIKHEYRYGENMFLGLDNAADADNMFDPETRLYAWQGRKMSGDELCDRYVELFY
                     KWTHQLAFEDPFAAPVSEWKFWRKLTEKLGDRLLIIGDDNVVTNPSVAYEALKENVCN
                     AGLIKLNQVGSFSQAWMYMEVFHSAGRNTVISHRSTQPDTIPDPLEVTATLAGSFRPQ
                     GRVVLAKLGGIFLANRAGLYYQMQKSAEEWRTGAAITPGVGPDVKIVSIAAYPSTLGA
                     GKYGLMAAMRLS"
BASE COUNT          754 a         1097 c         1035 g          786 t
ORIGIN      
        1 tagagcagcg gactcttaat ccgcgggtta taggttcaag tcctatacgg gtcatcttcc
       61 gttttattcc gcaattcccc gagtcggttt ttcattccct ttgatgcggg ctattcggat
      121 atgattattt tcgagtcatt gaactcaaca gtcgacgttc ttttataagt gttcttatcg
      181 taacgcgaga aagcgaatca gccaatgtta caaaatccgc aaaggcattt ccttttgaaa
      241 atgtctggga aagccacgcg acttctccgc ttgatcgatc atataatctt atttcaaatt
      301 tcatcctagg acggcttatg attcctcctc cgatcgcatt agtggtttgg ctgtaacgga
      361 catagttact gacaacccat actgatccgc tcgttatttg agtcggaggg acatatgttt
      421 gcgattcata ataatcagtc aaggaggcga cgagtatccc gtctatttgg tagcgatcca
      481 atacttggcg taattcgtag tcggaatagg atttgatcgg agagatcaaa tcaatgcctg
      541 caaaggaatc gacgtgggca tcgtaaaaag tatttgacag cttatattca aacatctttc
      601 tataacgcaa ttcttgaaac ggcgagatga ttaatatcct gccatatgct tttctttcat
      661 gatctggatt tgtaattgac gtgatatcgg tgaacgcaca tgatgtgacg actatgcaaa
      721 taacaataat agcggctttt ttcattcttt ccccccaata aaagtcaata gaaatattat
      781 tgcggccccc tgtaaagcct agcaaaatcc taagggagga gagacacgaa gtcaaggaaa
      841 ataacaattt tcgcaataat ccgttgcttt atcgtgtttg agataatgag acgtgaagaa
      901 tatcggcatc acgacccgcg ccttgacttc aaaacgctcc tggctgatga tatacctctt
      961 aaatgtcccg ggaggagaca tgaaaagacc ggttttcatc gccgccgcgg cggtttttgt
     1021 tacgattctg ggattagcct ccgtcggctg caagccggat ccggttccca agatcgtttt
     1081 gatcgagggc ttgaccctcg acgtcagtgt ctcgatgttc cgcgtcacgg ggcgcattca
     1141 gaaccagggc ggcggaaagg ccatgtacac ccgggtgaag atttatatcc gggacgcggc
     1201 cggcaacatt ctggcgtaca attggaacta ctgcgacgac accacgctgg aagcgggcga
     1261 gacggccggc ttcgaggtcc tcttctggga cgaggacggc aagctgcggg acaagatgga
     1321 cttttccaag tcgacatacg aattcgcgta cgactaaagc cgctccctct cttcaatccg
     1381 ccccttgccc catgatcccg cgggccggcc ctgtccgccg ccccggggac aagggagagc
     1441 gtctccgtct tgtcctgccc tccccttcca taatttcaat cccgggccgt tttttccgct
     1501 ttttgatccc gtcttccggc gttgacattt tccggatccc aggagtatct ttggcccata
     1561 attagttttt ttaaagattc cggaaaaggg atcattcatg gatttccgcg gccgcggcga
     1621 tcgtctcttg ccctcccggc cgcatgatcc ggggggagga tggacgtaaa atgaagcact
     1681 cgcattcgcc attcatccgc ttggaaccga ggagccgcgc cggcgggcgg ccccgttttt
     1741 cggaacggtt catagccgtc ctgatgcccc gaagctggcg cgaggggccg tttccggccg
     1801 cctggtccac ctcggaattc gtgaaatggg gatacttcgc cgtcgccctc cacgtccagg
     1861 ttctcaccag cctgttctat cccgaaatct ccgagagaat gtccgcctcg attcccgggt
     1921 tcgttgtcga attcggcgga tcggtcctcc tcggcgtcgc cttcgcctat ttcatgctgg
     1981 gcggccacca tgcccttcat gtcctggagg cggcccggac gtacaacctg gagcggaccc
     2041 tccaagcgac gatcggcgcc cggctggtcg agaagaaagc cgacggcacg gtccggttcc
     2101 ggaccgatac ggccgcctat tataaggaac tggctcgcat gtttcttctc gggccgtcgg
     2161 gccgccaccc ggctgtcatc aaggacggat tgaattaccg gctcaacggc acgccggctc
     2221 tcaacgtcgc cgccgcgggc tcccggttcg acctggccat ggcttatgtg gccggcctgt
     2281 cgggcctcgc cctcttcggt ttggcggtcg ccgcccggat cgacggccgg gaaaccggcg
     2341 gcgccctggc cttcatcact ccgcgaatcc tcgccctggg gatcatcgct ttcctgggcc
     2401 gccatcagga caacaacgcc ctgtaccggt accgggagaa gaaaaaaatc gaggctgacc
     2461 tggccgcccg ggccgcggcc ggcaccctga aagccgacgc cggccgcgag gcggtcgacg
     2521 cccgggcgaa atcgaattac gagcggctcg tcggagaagt gcgcaactcc gccgtttacg
     2581 gcgggagcga tccccggttc aaagggatcg ccgtttatct gcccgagggg gcccacaacg
     2641 cgatccaggc gggcaagcac accgaatacg tcgatgccga gggcaacaag ggcaacacct
     2701 cgcccgacct ggtcggccag gaaaagtgga tcatcctttt cccccgggaa acggaggagc
     2761 ccgcccgccg gggattcttc ggcacgctcc gggttctgcc gtcgctccag agccagatcg
     2821 tggccgagat cctcgcccgg gagggcgtct ccggcggcgg ccgggaaaac gagggcggat
     2881 gctcgttcaa cggctacggc ggcggcgacg aggcggccat cgacctgctg atggccgccg
     2941 cggccaaggc cgggatcaag cacgaatacc gctacggcga aaacatgttc ctgggcctcg
     3001 acaacgcggc tgacgccgac aacatgttcg accccgaaac gcgcctctac gcttggcagg
     3061 gccgaaagat gagcggcgac gagctttgcg accgctacgt cgagctgttc tacaagtgga
     3121 cccaccaact ggccttcgag gatcccttcg ccgccccggt gagcgagtgg aagttctggc
     3181 ggaagctgac cgaaaagctc ggcgaccggc tgctgatcat cggcgacgac aacgtcgtga
     3241 ccaatccgtc cgtcgcctac gaggccttga aggagaacgt ctgcaacgcc ggcctgatca
     3301 agctcaacca ggtcggctcc ttctcccagg cttggatgta catggaggtc ttccacagcg
     3361 ccgggcgcaa caccgtcatt tcccaccggt ccacccagcc ggacacgatc cccgatccgc
     3421 tggaggtcac ggccaccctg gccggttctt tccggccgca aggccgggtg gtcctggcca
     3481 agctgggcgg gatcttcctg gccaaccggg cgggcctcta ttaccagatg cagaaatcgg
     3541 ccgaggaatg gcggacgggg gccgcgatca cgcccggcgt cggcccggac gtgaagatcg
     3601 tgtcgatcgc cgcttatccc tccaccctgg gggcggggaa atacggattg atggccgcca
     3661 tgcgcctctc ca
//
LOCUS       DAOOXH010000153         3640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_30402_length_3640_cov_5.459693, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000153 DAOOXH010000000
VERSION     DAOOXH010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30402_length_3640_cov_5.459693"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1244
                     /locus_tag="PKV64_11315"
     CDS             <1..>1244
                     /locus_tag="PKV64_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HPN17704.1"
                     /translation="GTYTGIEAVSNGLGILREPRVHTGKKTMAYMAGSLAFTAGGILL
                     CYLLIGVRPVDGRTLNAVLADGVFSGWSIGGILAFITIFSEGALLLVGAQTGFVDGPR
                     VMANMAADYWFPHRFASLSNRFTIQNGVLMMGGSAILLLLYSRGHITTLVVMYSINVF
                     LTFTLSQLGMSRMFIRKRATEPKWKRHLPLHLVGLVVCLTILVITSIEKFALGGWLTF
                     LITGVVVLLCFLTRRHYNNVRRGVRELDELLSLPIDGPVNHDPVQPKATTAIQLVNGY
                     TGFGVHTFLSILRSFRRLYKQFIFVSVGEIDVTAFKGTDEVRKLEEATRRDLDQYVSL
                     ARRFGFPAAARMEMGPDVVATASSLIEAVSREFPDSIVFAGQSVFRKPGVIHRLLHNE
                     TAFAIQQELRWKGITTVILPIR"
     assembly_gap    1245..1344
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1471..2142
                     /locus_tag="PKV64_11320"
     CDS             1471..2142
                     /locus_tag="PKV64_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPN17705.1"
                     /translation="MTAFDAGAFRHIALEGVFHSGKTQWAGLLAERMGGKLVQDKTDN
                     PYLKDFYDEKDGAAFLAQLVFLVNRYHQQSRLVQRELFEERILCDYIFEKDKIYAYQT
                     LSDDELAVYDKIYGVFSERIPKPDLVIYLQIGLPTLLRRISRQGSPLDRNISEKYLQD
                     LIEAYDYFFFNYQATPLLVVKSDELDASRGDHLDDLIEQINGMKKTSLYYVPSSFDGA
                     AAKKR"
     gene            2203..3069
                     /gene="panB"
                     /locus_tag="PKV64_11325"
     CDS             2203..3069
                     /gene="panB"
                     /locus_tag="PKV64_11325"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPN17706.1"
                     /translation="MTPQQEPSRMTVPLLRARKGAGKIVALTAYDYAVASILDAAGAD
                     LLLVGDSLGMVLLGYENTLPVTMDEMINHTKPVARAARRALVVGDMPFLSYHLSAEQA
                     VANAGRFIKEGGAAAVKIEGASPRRLRLIETLVEADIPVLGHIGLTPQSIGRFGGFKV
                     QGTTPAGARRLVAEARAVEKAGAFGMVVECVPAEVAAGITAAVGIPTIGIGAGPGCDG
                     QIIVFHDLVGFHHGYMPKFVKTYADLHGVISEAVGRYRDDVRSGVFPDEAHSYHLKAG
                     TARGPKSGRARG"
     gene            3086..>3640
                     /gene="panC"
                     /locus_tag="PKV64_11330"
     CDS             3086..>3640
                     /gene="panC"
                     /locus_tag="PKV64_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPN17707.1"
                     /translation="MKIIATVEEMGRAVREERRRGRSISLVPTMGFLHEGHLSLVRLS
                     RAAADITVVSLFVNPTQFGPKEDLNTYPRDFERDAAMLRAEGADYLFAPAAEDMYASE
                     HRTFVEVRDLQDKLCGRSRPGHFRGVCTIVLKLFNLVQPDCAVFGQKDAQQALILKRM
                     TADLNVPVRMIVGPIVREADGLALS"
BASE COUNT          631 a         1126 c         1097 g          686 t
ORIGIN      
        1 gggacttaca ccgggatcga ggcggtctcc aacggcctgg gcatccttcg ggagccccgc
       61 gtccataccg ggaaaaagac gatggcctat atggccggat ccttggcctt tacggccggc
      121 ggcatccttc tctgctacct gttgatcggg gtgcggccgg tggacgggcg gacgctcaac
      181 gccgtcctgg ccgacggcgt gttcagcggc tggtccatcg gagggatcct ggcgttcatc
      241 accatcttct ccgagggcgc cctgttgctc gtcggggccc agacgggatt cgtcgacggg
      301 ccgcgggtca tggccaacat ggccgccgac tactggttcc cgcaccggtt cgcctccctc
      361 tcgaaccggt tcacgatcca gaacggcgtc ctgatgatgg gcggatcggc gatccttctg
      421 ctgctttatt cgcgcggcca tatcacgacc ctggtggtca tgtattcgat caacgtcttc
      481 ctgaccttca ctctcagcca gctcggcatg tcgaggatgt tcatccggaa gcgggcgacg
      541 gagccgaaat ggaagcggca tctgccgctc catctcgtcg gcctggtcgt ctgcctgacg
      601 attctcgtca tcacctcgat cgagaagttc gccttaggcg gctggctgac cttcctgatc
      661 acgggggtgg tcgtccttct ttgcttcctg acccgccggc actacaataa cgtccggcgc
      721 ggcgtgcggg agctggacga gctcctaagc ctgccgatcg acggcccggt caaccatgac
      781 ccggtccagc cgaaggcgac gacggccatt caactcgtca acgggtacac gggattcggc
      841 gtccatacgt ttttatcgat cctccgcagc ttccgccgcc tctacaagca gttcatcttc
      901 gtctcggtgg gggagatcga tgtgaccgcc ttcaagggga ccgacgaggt ccggaagctg
      961 gaggaggcca cccgccggga tctcgatcaa tacgtttccc tggcccggcg cttcggtttt
     1021 ccggccgcgg cccggatgga aatggggccg gatgtggtgg ccacggcttc gtccctgatc
     1081 gaggcggtga gccgggaatt tcccgattcc atcgttttcg ccggtcagtc cgttttccgg
     1141 aagccgggcg tcattcatcg ccttctccac aacgaaacgg cgtttgccat ccagcaggag
     1201 ctccggtgga aggggatcac gacggtgatc ctgcccatcc ggatnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnntccagc aggagctccg gtggaagggg atcacgacgg
     1381 tgatcctgcc catccggatt aacatttaag ggaggccgcg ttgacttcgc ccctgttctt
     1441 cattataaaa aaagcgggag acgagcgttc atgaccgcct tcgacgccgg cgcattcagg
     1501 catatcgccc tggagggcgt tttccattcg gggaagacgc aatgggccgg gcttttggcc
     1561 gagcggatgg gcggaaagct cgttcaggac aagaccgaca atccctatct caaggatttc
     1621 tacgacgaaa aagacggggc cgccttcctg gcccagctcg tgttcctggt caaccgctat
     1681 caccagcagt cccgccttgt ccagcgggag ctcttcgagg agcggatcct ttgcgactat
     1741 atcttcgaaa aagacaagat ctacgcttac cagacgctgt cggacgacga gctggccgtt
     1801 tacgataaga tctacggcgt gttttccgaa aggattccca agcccgacct cgtcatctat
     1861 ctccaaatcg gcctgcccac gctgctgcgc cggatcagcc gccagggatc gccgctcgac
     1921 cggaacatct cggaaaagta tcttcaagac ctcatcgagg cttatgatta ttttttcttc
     1981 aattaccagg ccacgcccct tctcgtcgtc aagtccgacg agctcgacgc gtcccgcggg
     2041 gatcacctcg acgatttgat cgagcagatc aacggaatga agaaaacgtc cctctattat
     2101 gtcccgtcca gcttcgacgg agcggcggcg aagaagagat gagcgattga gactttaccc
     2161 caaccccggt ttttaagacg gggtggggag aaaggataaa acatgacccc ccagcaagag
     2221 ccctcccgaa tgacggtccc cctcctccgg gcccgcaagg gcgccggcaa gatcgtggcc
     2281 ctcacggcct acgattacgc cgtcgcttcc atcctggacg cggccggcgc ggacctcctt
     2341 ctcgtcggag attccctcgg catggtcctc ctcggctacg aaaacacgct tccggtgacg
     2401 atggacgaga tgatcaatca cacgaagccg gtcgcccggg ccgcccggcg agccctggtc
     2461 gtgggcgaca tgccgttcct ctcgtatcat ctctccgccg agcaggccgt ggccaacgcc
     2521 ggccggttca tcaaggaggg gggggcggcg gccgttaaga tcgagggcgc ctcgccccgg
     2581 aggttgcggc tgatcgaaac cttggtcgag gccgacattc ccgtcctcgg ccatatcggg
     2641 ctcacgcccc agtccatcgg ccgcttcggc ggcttcaagg tccaagggac gacgccggcc
     2701 ggagcccggc ggctcgtggc cgaggcccgg gccgtggaaa aagccggggc tttcgggatg
     2761 gttgtggagt gcgtcccggc ggaggtcgca gcggggatta cggccgcggt cggcattccc
     2821 acgatcggca tcggagccgg gcctggctgt gacggccaga tcatcgtttt tcacgacctg
     2881 gtcggctttc accatggcta catgcccaaa ttcgtcaaga cctacgccga cctccacggg
     2941 gtgatttcag aagcggtcgg ccgctaccgg gacgacgtcc ggtcgggtgt cttcccggac
     3001 gaggctcatt cctatcacct gaaagccggg acggcccgcg ggccgaaaag cggccgggcg
     3061 cgcggctgag ccccggagaa aagccatgaa gatcatcgcg acggtcgagg aaatgggccg
     3121 ggcggtccgg gaagagcggc ggcggggacg ttcgatcagc cttgtcccga cgatgggatt
     3181 cctgcatgag gggcatttgt cgctggtccg gctctcccgg gccgcggccg acatcactgt
     3241 cgtcagcctg ttcgtcaatc cgacgcaatt cgggcccaag gaagatttga acacctaccc
     3301 gcgcgatttc gagcgggacg cggcgatgct tcgggccgag ggcgcggatt acctgttcgc
     3361 cccggcggct gaagacatgt atgcctcgga acaccggacg ttcgtcgagg ttcgggacct
     3421 ccaggacaag ctttgcgggc ggtcccgccc cggtcatttc cggggcgtct gcacgatcgt
     3481 cctgaagctg ttcaacctgg ttcagccgga ttgcgccgtc ttcggccaaa aagacgccca
     3541 gcaagccttg atcctcaagc ggatgaccgc cgacttgaac gtccccgtcc ggatgatcgt
     3601 cgggccgatc gtccgggaag ccgacggcct ggccctgagc
//
LOCUS       DAOOXH010000154         3543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_31575_length_3543_cov_5.987099, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000154 DAOOXH010000000
VERSION     DAOOXH010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31575_length_3543_cov_5.987099"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PKV64_11335"
     CDS             complement(<1..586)
                     /locus_tag="PKV64_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPN17708.1"
                     /translation="MNKRMILWGLAAALALAAGAFSQEAKKMTYPVTKTVDQVDDYFG
                     TKVADPYRWLEDDRSAETEAWVKAQNAVTFGHLEQIPFREALKKRLMDIYNYPRYSAP
                     FRVGGHYFFSKNDGLQNQSVIYIQKGLEGTPEVFIDPNALSADGTIKIYLLGPSGCKT
                     KMAIMRGEAGSDWGEIRVMDIASKTELPDRIRWVK"
     gene            complement(619..1941)
                     /locus_tag="PKV64_11340"
     CDS             complement(619..1941)
                     /locus_tag="PKV64_11340"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPN17709.1"
                     /translation="MSPRNWTATEEIFRLFQAVGRASLLADIQDSHSGNMAVKVKGED
                     GSDVIVITAAGSQKGDLEPEQICFLSATETDYGYYKASSETDIHQRILRLPGVGASMH
                     AHTKELAIATLDDGPKPARPPDFVPIDSLGRHVLGERVAVDWFAVPSGSPEMAETVPW
                     RLAGRPVTTIQGHGTFARGRTLQEAFFLLAAADNAGKVVNAARRLKVDVEGLRAGMLA
                     RPSDFFVRPPDPYAVEDDEACDFPEETEILKEFRKAGARIFESFLSPFHTGSMSVRGV
                     GDLLYAPKASMPRGLPGPLRRRPIRPEGTDSPELALHKAIYAESDFQTVMHCWLPEAD
                     AHAYFRYPGEKTAADRIVPVDAEGGFQYLVIPVLPADAGPEALIRGLHDYKVAVIRGG
                     GVWAAGHQSLSEVLHHPSSVREICLYRIGAFERGLDLRRMEPAKAKKW"
     gene            complement(1938..>3543)
                     /locus_tag="PKV64_11345"
     CDS             complement(1938..>3543)
                     /locus_tag="PKV64_11345"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HPN17710.1"
                     /translation="SPLSGLIGTSNSGGSWGFAFKATGHDALVIKGKAPEPVWIDITP
                     AGAAIHPAGTLWGEDIHATTDALTSGFSPADRPRVLCIGPAGENLVRFADVANDRNRV
                     YGRGGPGAVWGSKNLKAVRVSGKEKIRIRDPERYKSGYDQAFHLLRQAPVTKRLLREL
                     GTAGLVELINVIGMLPRRNFQDCAHRDEDLERISGETIARTILERAAGCHLCPIGCQR
                     RTRIVRAGSPDESGEGPEYETVVLMGPVCDVYDLAAITTANYRCNELGLDTISFGGTV
                     ACAMELAEAGALPPGFDGGRGLRFGNAAVLERLAVDVAHRLGPGDLLAEGSWRLASSC
                     GHPEASMSVKRMEIPAYDPRASFTQALGYMTSPSGACHLRGGYAVSLAFFGGAKEIPR
                     FSLLQSPIAIRNMQNIGILQDSLGVCRFTGYAFGSDPWSRMLSGATGMDFSVARLEEI
                     ANRIADLERLFNLEAGAVPSDDALPARFAESPVVVAGENRIVSRETQERMRRDYYQVR
                     GWDANGRPAAGPRPPRRLDPPEGRRP"
BASE COUNT          597 a         1172 c         1167 g          607 t
ORIGIN      
        1 atttcaccca gcggatccgg tcgggaagct cggtcttgga ggcgatgtcc atcacccgga
       61 tctcgcccca atccgatccg gcttctccgc gcatgatcgc cattttcgtc ttgcagccgg
      121 agggaccgag gagatagatt ttgatggttc catcggccga aagcgcgttc ggatcgatga
      181 acacctcggg cgtcccctcg aggcctttct ggatatagat gacggactgg ttttgaagcc
      241 cgtcgttctt ggagaagaaa taatgcccgc cgacccggaa cggcgccgaa taccggggat
      301 agttgtagat atccatcagc cgcttcttca gcgcctcgcg gaaggggatc tgttcgagat
      361 ggccgaacgt cacggcgttc tgggccttga cccatgcctc ggtctcggcc gaacggtcgt
      421 cttcaagcca gcggtacggg tcggcgactt tcgtcccgaa ataatcatcg acctggtcga
      481 cggtcttggt cacggggtag gtcatctttt tcgcctcttg actgaaggct ccggcggcca
      541 gcgccagggc ggccgccaag ccccacagga tcattctctt attcatcaaa atctccttga
      601 tggacggata tttttattct accacttttt cgccttggcg ggctccatcc ggcgcaggtc
      661 caggccgcgc tcgaaggcgc cgatccggta gagacagatt tccctcaccg acgacggatg
      721 gtggaggacc tccgagagcg actggtgacc ggccgcccag actccgccgc cccggatgac
      781 ggccactttg taatcatgca ggccgcggat cagggcctcc gggccggcgt ccgccggcag
      841 gacggggatg acgagatact ggaagccgcc ctcggcgtcc accggaacga tccggtcggc
      901 cgcggtcttc tcgcccgggt accggaaata agcgtgagcg tccgcctccg gcagccagca
      961 atgcatgacc gtctgaaaat ccgattcggc gtagatcgcc ttatgaaggg ccagctcggg
     1021 cgagtcggtt ccttccggcc ggatcggccg ccggcgcagc ggcccgggaa ggccgcgcgg
     1081 catcgacgcc ttcggggcgt acagcagatc gccgaccccg cggaccgaca tcgagccggt
     1141 gtggaacggc gagaggaacg attcgaagat cctggccccg gccttgcgga attccttaag
     1201 gatttcggtc tcctcgggga aatcgcaggc ctcgtcgtcc tcgaccgcgt agggatcggg
     1261 cggccggacg aagaagtccg agggccgggc gagcattccg gcccgaagcc cttccacgtc
     1321 caccttcagc cgccgggcgg cgttgacgac cttcccggcg ttgtcggccg cggccagaag
     1381 aaaaaaagcc tcctggagcg tccggccccg ggcgaacgtc ccatggcctt ggatcgtcgt
     1441 caccggccgg ccggccaggc gccacggcac cgtttcggcc atctccggcg agccgctcgg
     1501 cacggcgaac cagtcgacgg ccacccgttc cccgaggacg tgccggccca aggaatcgat
     1561 gggaacaaag tccggcggcc gagcgggctt cggcccgtca tccagggtgg cgatcgccag
     1621 ctccttcgtg tgggcgtgca tggacgcgcc gacgccaggg aggcggagga tccgctgatg
     1681 gatgtcggtc tcggaggagg ccttgtagta tccgtaatcc gtttccgtcg ccgaaaggaa
     1741 gcagatctgc tccggctcga gatcgccctt ctgggaaccg gccgcggtga tgacgatgac
     1801 gtcggatccg tcctcgccct tgaccttgac ggccatattg ccggaatggc tgtcttggat
     1861 gtcggccaga agggacgccc gtccgaccgc ctgaaacagc cggaagatct cctcggtcgc
     1921 ggtccagttt cgagggctca tggccgcctc ccttcgggtg gatcgagacg gcgcggcggc
     1981 ctcggcccgg cggccggacg gccgttcgcg tcccagccgc ggacttggta ataatcccgg
     2041 cgcatccgct cctgggtttc ccggctgacg atccggtttt ctccggcgac gacgaccggc
     2101 gattcggcga accgggccgg gagcgcgtcg tcagatggaa cggccccggc ctccaggttg
     2161 aacaggcgct caaggtcggc gatccggttg gcgatctcct cgaggcgggc aaccgaaaaa
     2221 tccatgccgg tcgctccgga gagcatccgc gaccagggat cgctcccgaa ggcgtacccg
     2281 gtgaaacggc agacgcccag gctgtcctgg aggatcccga tattctgcat gttccggatg
     2341 gcgatcgggg actggaggag gctgaagcgg gggatttcct tggccccccc gaaaaacgcc
     2401 agggaaacgg catagccgcc gcgaagatga caggctcccg acggcgaggt catgtaaccc
     2461 aacgcttggg tgaacgaagc ccggggatcg tatgccggga tctccatccg tttgaccgac
     2521 atggacgcct cgggatggcc gcagctcgag gccagccgcc aggatccctc ggctaaaaga
     2581 tcccccggcc ccaggcgatg ggcgacatcg acggccagcc gctccaggac ggcggcgttt
     2641 ccgaagcgca gtccccggcc gccgtcgaaa cccggcggca gggcgccggc ctcggccagc
     2701 tccatggcgc aggcgaccgt tccgccgaag ctgatcgtgt ccaaacccag ctcgttgcag
     2761 cggtaattgg ccgtcgtgat ggccgccagg tcgtagacgt cgcagaccgg ccccatcagg
     2821 acgacggttt cgtactccgg accctctccg gattcgtccg gcgatcccgc ccggacgatc
     2881 cgggtgcggc gctgacagcc gatgggacag agatggcagc cggcggctcg ctccagaatg
     2941 gtccgggcga tggtttcgcc cgagatccgc tccaggtcct cgtcccggtg agcgcaatcc
     3001 tggaaattgc ggcggggcag catcccgatg acgttgatga gctcgaccag gccggccgtc
     3061 ccgagttcgc gcaggagccg cttggtcacc ggggcctgcc gcaggagatg gaacgcctga
     3121 tcatatcccg acttgtagcg ctcgggatcc cggatccgga ttttttcctt tccggagacg
     3181 cggaccgcct tcaggttttt tgaaccccag accgcgcccg gtccgccgcg cccgtaaacc
     3241 cggttgcggt cattggccac gtcggcgaac cggacgagat tctccccggc cggaccgatg
     3301 cagaggacgc gcggccggtc ggccgggctg aacccggagg tcagggcgtc cgtcgtcgcg
     3361 tggatgtcct ccccccaaag cgtcccggcc gggtggatcg ccgcgcccgc cggcgtgatg
     3421 tcgatccaga ccggctccgg cgcttttccc ttgatgacca gggcgtcgtg gcccgtggcc
     3481 ttgaaggcga atccccagct tccgccggaa ttcgacgtcc cgatcaagcc cgaaagcggc
     3541 gag
//
LOCUS       DAOOXH010000155         3518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_31891_length_3518_cov_5.412070, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000155 DAOOXH010000000
VERSION     DAOOXH010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31891_length_3518_cov_5.412070"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2036
                     /locus_tag="PKV64_11350"
     CDS             <1..2036
                     /locus_tag="PKV64_11350"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPN17711.1"
                     /translation="FITRFSILVLIGLAASSAAADHRFLNLMPRPSNVLLTEGRFPLT
                     ESFTVGALGQPGDRARRAADRFLERLSGRTGLVLRKEVPLPEGGESGAALLFSCERTG
                     ELLPGEDESYRLTVGPDRILLVAPTDLGILHGFETALQLLASGPDGYFFPCVQIEDGP
                     RFPWRGLLIDSARHFHPVDVIKRNLDGLAAVKMNVLHWHLTEDQGFRVESKVFPALHR
                     LGSDGLFYSQDQIRDIIAYAADRGIRVMPEFDLPGHSTSWFPAFPELASAPGPYRISR
                     TYGVQLPAFNPANSQVYVFLDAFLGEMAGLFPDPYIHIGGDENNGKHWDANPEIQAFM
                     KEKGLADNAALQAYFNGRLFEILAKHGRKMVGWDEIFQPGLSKDVVIQSWRGTEALVQ
                     AARRGYAGLLSNGYYVDLCYSAADHYLNDPLPPGHGLSEAEAALVLGGEATMWSELVS
                     PETIDSRIWPRTAAIAERLWSPADTRDVDDMYRRLEPLSIQLEELGLTHLKNKDMLLR
                     RLVRNMEVGPLRLLADAVEPLKGYKRHTVGKVQTTLAPLTRFVDAVPPESLTARHFSR
                     IAAAFLDRRDAGSAEALRTMLETWRMNHDLIVPLLPSSPVLKEIEPLSRGLAETAHVG
                     LEAIEALVRKHKIRHSQSAEWQAALAEWKKPLAELELAILPAVEKLVAAVAIR"
     gene            2104..>3518
                     /locus_tag="PKV64_11355"
     CDS             2104..>3518
                     /locus_tag="PKV64_11355"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPN17712.1"
                     /translation="MMNHRLLSSRRRIAAAAALFVVAVGGPALFLFPQKTEKPVLHGR
                     HWAAVTGKPMAATAGAMIFQKGGNAVDAACAMLGAVCTMWDVLSWGGETQALIYHPVQ
                     KKVIAVNALGAAPAGAVAAYFRDRGMRHPPATGPLAAVTPGTPGGLMTMLAEFGTMSL
                     EEVLAPAMSLAEGYPMEKGTADSIEREKDKIKAWPYARAVMLPHAGRDREGPVPGEIF
                     RQDDLLETLGKLVQAEKDARAAGRSRKEAIYAAYDRFYKGDIGREFVRGANEQGALFG
                     EDDLARWRVYLEEPVKTTYKGLEVYKLNTWVQGPVLLQALNMLENIDLKKLGYNSAAY
                     IHTICQVMNLAFADRDFYYGDPYYPPAEPLAGLLSKDYARERLRLLNPERNDPGVKPG
                     DPYPFQGQVNPFRKILEAWPPADEPTLNKDPDPASFATGTTSIQAADADGWVVSVTPS
                     GGWIPAVIAGRTGIGLSQRAQ"
BASE COUNT          600 a         1176 c         1132 g          610 t
ORIGIN      
        1 gattcatcac ccggttttcc atcctggttc tgatcggcct ggcggcctcg tccgccgccg
       61 ccgatcatcg ctttctcaac ctgatgcccc ggccctcaaa cgtccttttg accgaagggc
      121 ggtttccctt gaccgagtca ttcaccgtcg gagcgctcgg ccagcccggc gaccgcgccc
      181 gccgggcggc cgaccggttc ctggagcggc tcagcggacg gacggggctc gtcctccgaa
      241 aagaggttcc cctcccggaa ggaggggaaa gcggggcggc cctgcttttt tcctgcgagc
      301 ggaccggcga gctcctgccc ggcgaggatg agtcctaccg tctgaccgtc gggcctgacc
      361 ggattctcct ggtcgctccg acggatctgg ggattctcca cgggttcgag acggcccttc
      421 aattgctcgc ctcagggccg gatggatatt ttttcccttg cgtccaaatc gaggacggcc
      481 cccgtttccc ctggcgcggg cttttgatcg acagcgcccg tcattttcac ccggtcgatg
      541 tgatcaagcg gaacctcgac ggcctggccg cggtcaagat gaacgtcctc cactggcatt
      601 tgacggagga ccagggattc cgggttgaaa gcaaggtctt ccccgctctc caccggctcg
      661 gctcggacgg gcttttttac tcccaggacc agatccggga catcatcgcc tacgccgcgg
      721 accgcggcat ccgggtgatg ccggaattcg atcttccggg gcattcgacg agctggttcc
      781 cggcttttcc cgagctggcc agcgcgcccg gcccctaccg gatttcccgg acctacggcg
      841 ttcaactgcc cgctttcaac ccggccaatt cccaggtcta cgtcttcctc gacgcgttct
      901 taggggagat ggccgggctg ttccccgatc cttatattca catcgggggg gacgaaaaca
      961 acggcaagca ctgggacgcc aatcccgaaa tccaggcgtt catgaaggag aagggcttgg
     1021 ccgacaacgc cgccctccag gcctatttca acggccggct gttcgagatc ctggccaagc
     1081 acggccggaa aatggtcggc tgggacgaga tctttcagcc ggggctgtcc aaggatgtcg
     1141 tcatccaatc ctggcggggg accgaagccc tcgtccaggc cgcccggcgg ggatacgccg
     1201 gcttgctctc caacggctat tacgtcgatc tctgttattc cgcggccgat cactacctga
     1261 acgaccctct tccccccgga cacggccttt cggaggccga ggcggccctc gtcctgggcg
     1321 gggaggcgac gatgtggagc gagctggtct cgcccgagac gatcgattcg cgcatctggc
     1381 cgcggacggc ggccatcgcc gaacggctct ggtcgccggc cgacacccgc gacgtggacg
     1441 acatgtaccg gcggctggaa cctctttcga tccaactgga agaactgggc ttgacccatc
     1501 ttaaaaataa agacatgctg ctccggcgtc tcgtccggaa catggaggtc ggaccgctcc
     1561 ggctgctggc cgacgccgtc gagccgctca agggctacaa gcgccatacg gtgggcaagg
     1621 tccagacgac gctggccccg ctcacccggt tcgtcgatgc cgttccgccg gaatccctga
     1681 ccgcccgcca tttcagccgg atcgcggccg cgttcctcga ccggcgcgac gccggatcgg
     1741 cggaagcgct ccggacgatg ctggagacct ggcggatgaa tcacgatttg atcgtgccgc
     1801 tcctgccctc ttctcccgtc ctcaaggaaa tcgagccgct gtcccgcggg ttggcggaga
     1861 cggcgcatgt cggcctggag gcgatcgagg ccctggtccg caagcacaag atccgtcatt
     1921 cgcagtcggc cgaatggcag gccgcgctgg ccgaatggaa aaagccgctg gccgagctcg
     1981 agctggccat tctcccggcg gtcgaaaagc tggtcgcggc tgttgccatc aggtgaggcc
     2041 ggcccggatc ttttccggat cccggccgtg gatcgaacgc ccccgggcga acggagagga
     2101 gggatgatga accaccgcct tctatcatca cggcgccgga tcgcggcggc cgcggcctta
     2161 ttcgtcgttg ccgtcggcgg cccggccctt ttcctttttc cgcagaaaac ggaaaaaccg
     2221 gtccttcacg gccgccattg ggcggccgtc accggcaagc cgatggccgc cacggccggg
     2281 gcgatgatct tccagaaggg cggaaacgcg gtggacgcgg cctgcgccat gctcggggcc
     2341 gtctgcacga tgtgggacgt cctcagttgg ggcggcgaaa cgcaggccct gatctaccat
     2401 ccggttcaaa aaaaggtcat cgccgtcaat gccctcgggg cggctccggc gggggctgtg
     2461 gccgcgtact tccgggatcg tgggatgcgc catccgccgg cgaccggacc gctcgcggcc
     2521 gtcacgcccg gtactcccgg cgggctgatg acaatgctgg ccgagttcgg gacgatgagt
     2581 ctggaggagg tgcttgcccc ggccatgagc ctggccgagg gctatcccat ggaaaaaggc
     2641 acggccgact cgatcgagcg ggagaaggac aagatcaagg cctggccgta cgcccgggcg
     2701 gtgatgctgc cgcacgccgg ccgggaccgg gaagggccgg ttccgggcga gatcttccgc
     2761 caggacgacc tcctcgaaac gctcggcaag cttgtccagg ccgaaaagga cgcccgggcc
     2821 gcgggccgga gccgcaagga ggcgatctac gccgcctatg accgtttcta caagggcgac
     2881 atcggacggg aattcgtccg cggggccaac gagcagggcg ccttgttcgg agaggacgac
     2941 ctggcccgct ggcgcgttta cctcgaggaa cccgtcaaga cgacttacaa ggggctggag
     3001 gtctataagc tcaacacctg ggtccagggg ccggtcctgc tccaggctct gaacatgctc
     3061 gaaaacatcg acctgaaaaa gctgggatac aactcggccg catacatcca caccatctgc
     3121 caggtgatga acctggcctt cgccgatcgc gatttttatt acggcgatcc ctattatccg
     3181 cccgcggagc cgctggccgg gcttctctcc aaggactacg cccgggagcg gctgcggctt
     3241 ctcaacccgg agaggaacga tcccggggtc aagcccggag acccctatcc gttccagggg
     3301 caggtcaatc ctttccggaa aatcctggaa gcctggcctc cggccgatga gccgactctg
     3361 aataaagatc ccgatccggc aagcttcgcg accgggacga cgtccatcca ggccgccgac
     3421 gcggacggct gggtcgtctc ggtcacgccg agcggcggct ggatcccggc ggtcatcgcc
     3481 ggccggacgg ggatcggcct cagccagcgg gcccagag
//
LOCUS       DAOOXH010000156         3476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_32424_length_3476_cov_8.326513, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000156 DAOOXH010000000
VERSION     DAOOXH010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32424_length_3476_cov_8.326513"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..2604
                     /gene="gyrB"
                     /locus_tag="PKV64_11360"
     CDS             214..2604
                     /gene="gyrB"
                     /locus_tag="PKV64_11360"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPN17713.1"
                     /translation="MTKEKYSAESIKVLKGLEAVRKRPAMYIGSTGSEGLHHLVYEVV
                     DNSIDEALAGFCKDIEVFIHVDDSVTVLDDGRGIPVAMHSKEKKSAAEVVMTILHAGG
                     KFDNKSYKVSGGLHGVGVSVVNALSRRLDLEIKRDGGIFVQSYERGVAVARLKQVGKT
                     KKTGTKITFWPDDEIFDSTEFSFEILTQRLRELSFLNRGIRISITDERISKSHEFQYK
                     GGIVEFVQFLNQNKNVLNPRPIFFESKKDDVIVELAFQYNSGYSETIFSFVNNINTHE
                     GGTHLIGFKSALTRCLNSYAESHNLLKDLGAGSLGGDDAREGLCAVLSVKMRDPQFEG
                     QTKTKLGNSEVKGIVESLVNEQLGAYLEENPTNAKRIILKAVEAARAREAARKARDLA
                     RRKSVLESSSLPGKLADCQERDPALAEIFLVEGDSAGGSAKQGRDRRFQAILPLKGKI
                     LNVWKARQDKIFNNDEIGTMIAALGTTLGEEESSLEKLRYHKVIIMTDADVDGSHIRT
                     LLMTFFYRHMKSLIEEGHLFIAQPPLYKIVRGKEVTYLKEERAYEKWIVKKISEEFKI
                     RVKGAKEIFEGEKFRKLFLRIMQKRNYIQLLERKSYPFFLLERLIHEGVNGLEFLQDK
                     KAVKRLQSLLQKEYDLDVEMTHDEEYDAWELAVKFGVNGMTVVCKVNWNLIDSAEYKA
                     LFKIFQDLADFKAPFEVLSNGECIVVENETKLLEYLHEKGKKGVSIQRYKGLGEMAPE
                     QLWSTTMNPENRALLRVSITDAVTADEVFTILMGDEVERRRNFIETNAAHVTNLDI"
     gene            2863..>3476
                     /locus_tag="PKV64_11365"
     CDS             2863..>3476
                     /locus_tag="PKV64_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPN17714.1"
                     /translation="MKRSYLDYAMSVIIGRAIPDIKDGLKPVHRRVLYAMYETGTVYN
                     KPHKKSARIVGDVIGKYHPHGDQAVYDTLVRMAQDFSLRALLVDGQGNFGSIDGDPPA
                     AMRYTEVRMTKLAGEMLADIEKDTVNFIPNYDESLTMPEVLPTKFPNLLVNGSSGIAV
                     GMATNIPPHNLGEISNGIVHLLDHPDASLEDIMKLVPGPDFPTGG"
BASE COUNT          804 a         1117 c          961 g          594 t
ORIGIN      
        1 agaaccgcgg aaaaagcctc ccctccgttc cgcccggttc tcccaaggcc ttgggccgga
       61 tcctcccctc cgccccaaaa tattgtgttt ttgatcgaaa atttcgcttt actttttccc
      121 tcgattcagg gtataattca gacatgaaat ccgcggcgtt tttttaagag cgatacactt
      181 ataactcact tgaattcaat gagatagaat caaatgacaa aagagaagta ttcggcggaa
      241 agcatcaagg tcctcaaagg cctcgaagcg gtccggaaac ggcccgcgat gtacatcgga
      301 agcaccggct ccgagggcct ccatcacctg gtttacgaag tcgtcgacaa ctccatcgat
      361 gaggccttgg ccggcttctg caaggacatc gaggtcttca tccacgtgga cgactcggtg
      421 accgtcctcg acgacggtcg cggcatcccg gtggccatgc actcgaaaga aaagaagtcg
      481 gcggccgaag tggtcatgac catccttcac gccggcggca aattcgacaa caagtcctac
      541 aaggtctccg gcggactcca cggtgtcggc gtctcggtcg tcaacgcctt gtcccggcgc
      601 ctggacctgg aaatcaagcg ggacggcggc atcttcgtcc agagctacga acgcggcgtt
      661 gccgtggccc gcctcaagca ggtcggcaag acaaagaaaa ccgggaccaa gatcacgttc
      721 tggcccgatg acgagatctt cgattcgacg gagttcagct tcgaaatcct gacccagcgc
      781 ctccgggagc tgtccttcct caaccggggc atccgcatct cgatcacgga cgagcggatt
      841 tccaagagcc atgaattcca gtacaagggc ggaatcgtcg aattcgttca gttcctcaac
      901 cagaacaaaa acgtcctcaa tccgcgcccg atcttcttcg agtcgaagaa ggacgacgtc
      961 atcgtcgagc tggctttcca gtacaactcc ggctacagcg agacgatttt ctccttcgtc
     1021 aacaacatca acacccacga gggcggcact cacctgatcg gctttaaatc cgccctgacc
     1081 cgctgtctca attcctacgc cgaatcgcat aacctcctga aggacctcgg ggccggctcg
     1141 ctcggcggcg acgacgcccg ggaggggctg tgcgccgttc tcagcgtcaa aatgcgcgac
     1201 ccccagttcg agggccagac caagaccaag ctgggcaatt ccgaggtcaa gggcatcgtc
     1261 gaatcgctgg tcaacgagca gttgggcgcc tacctcgagg aaaatccgac caacgccaag
     1321 cggatcatcc tcaaggccgt cgaggccgcc cgggccaggg aagccgcccg caaggcccgc
     1381 gacctggccc ggcgcaagag cgtcctggaa tcctcgtccc tccccggcaa gctggccgac
     1441 tgccaggagc gcgatccggc cctggccgag atcttcctgg tcgaggggga ttcggccggc
     1501 ggctcggcca agcagggccg cgaccgccgc ttccaggcca ttcttcccct gaagggcaag
     1561 atcctcaacg tctggaaagc ccggcaggac aagatcttca acaacgacga gatcgggacg
     1621 atgatcgccg ccctgggcac gaccctgggc gaggaggaat cgagcctgga gaagctccgc
     1681 taccacaagg tcatcatcat gaccgacgcg gacgtggacg gctcccacat ccggacgctt
     1741 ctcatgacgt tcttctaccg ccacatgaaa tcgttgatcg aggaaggcca ccttttcatc
     1801 gcccagccgc ccctgtataa gatcgtccgg ggcaaggagg tcacctactt gaaagaggag
     1861 cgggcgtacg aaaagtggat cgtcaagaag atctccgagg aattcaagat tcgggtcaag
     1921 ggcgccaagg aaattttcga gggcgagaaa ttccgcaagc tcttccttcg gatcatgcag
     1981 aagaggaatt acatccagct cctggagaga aaatcgtatc ccttcttcct tctcgagcgg
     2041 ctcatccacg agggcgtcaa cggcttggag tttctccagg acaagaaggc ggtcaagcgc
     2101 ctccaatccc tcctccaaaa ggaatacgac ctggacgtcg aaatgaccca cgacgaggaa
     2161 tacgacgcct gggagctggc cgtgaaattc ggcgtcaacg ggatgacggt cgtctgcaag
     2221 gtcaactgga acctgatcga ctcggccgag tacaaggccc ttttcaagat tttccaggat
     2281 ctggccgatt tcaaggcgcc gttcgaggtc ctgtccaacg gcgagtgcat cgtcgtcgag
     2341 aacgagacca agctcctcga gtacctccat gagaagggga aaaaaggcgt ctccatccag
     2401 cgctacaagg ggttgggcga aatggccccc gaacagctct ggtcgacgac gatgaacccg
     2461 gagaaccggg ctctcctccg cgtctcgatc accgacgccg tgaccgccga cgaggtcttt
     2521 acgatcctga tgggcgatga agtcgaacgc cgccgcaact tcatcgagac caacgcggcc
     2581 cacgtcacca acctggacat ttaacccatg gctaaaaaag aaaaagaaaa agagaaagag
     2641 agagaaaaaa ccaagcccaa gatcgcggcc aaaggcggcc gcggaggccg gggaaaaacc
     2701 ccggttccgc cgcctgtccc ggagccggag atcccggctt ccgccgtccc gggcggagac
     2761 gccccggccg aaaaaacgcc ggcccgggaa aaagctccgt ccgcccaaca gccgctcttt
     2821 ccggccgcgg ccgagctgtc caacgtcgcc ctcgaggagg agatgaagcg ctcctacctc
     2881 gattacgcca tgagcgtcat catcggccgg gccatccccg acatcaagga cgggctcaag
     2941 cccgtccacc ggcgcgtcct ttacgcgatg tacgaaacgg gcacggttta caataagcct
     3001 cacaagaaat cggcccggat cgtcggcgac gtcatcggaa aatatcatcc tcacggcgac
     3061 caggccgtct acgacaccct cgtccggatg gcccaggatt tctcgctccg ggccctgctc
     3121 gtcgacggcc agggcaactt cggctcgatc gacggcgatc cgccggcggc catgcgctac
     3181 accgaggtcc ggatgaccaa gctggccggc gagatgctgg ccgacatcga gaaggacacg
     3241 gtcaacttca tccccaacta cgatgaaagc ctgaccatgc ccgaggtcct gccgaccaag
     3301 ttccccaacc tcctggtcaa cggaagctcg ggaatcgccg tcggcatggc caccaacatc
     3361 ccgccccata acctgggcga gatctccaac ggcatcgtcc acctcctgga tcatcccgat
     3421 gcctcgctcg aagacatcat gaagcttgtc cccgggccgg atttccccac cggcgg
//
LOCUS       DAOOXH010000157         3464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_32578_length_3464_cov_5.166911, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000157 DAOOXH010000000
VERSION     DAOOXH010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32578_length_3464_cov_5.166911"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3015)
                     /locus_tag="PKV64_11370"
     CDS             complement(<1..3015)
                     /locus_tag="PKV64_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S9"
                     /protein_id="HPN17715.1"
                     /translation="MKSSTGEHKPSGVRFRRIVFLLIFGATAVTASAQYFGRNKVQYE
                     KFDYQVLTTDHFQIYYYPEEEAAVRTAARMAERWYNRLARIFNHDLKGKQALIMYASH
                     PQFEQTTVLSELISEGTGGVTESFKRRIILPFGASLDDTDHVIGHELVHAFQYDMALG
                     GEGRANPQGGEGLARTPLFLIEGAAEYFSIGPEDPNTAMWMRDMIRRKKIPALKDLEN
                     YYKYFPYRYGQAVYAYIGGRWGDLVAARLMRDVVRGYDYRKAFTRVLGVTPEQFSKDW
                     HEALKAEYGLIEKSTEGPLDKAQVVVKGTEDVSLNCAPAISPDGKHFVFISSRDLFSI
                     EMFLGDALTGKITRKLTKTSIDPHFQSIQFIYSAGSWSADGTQFAFGSVIAGKPALSF
                     LDVGGRRLKDDIVFPHLGEILNPTWSPDGRKIAFSALTGGCSDLFIYDLETQTLLNMT
                     SDPYGDLQPAWSPDGKWIAFITERFASDLSLVGSGGFHLALLDPETGEIRELPAFQAG
                     KHISPQWSADGRQLYFVSDRDGVSNLYRLDRNTGRFYQITNLYTGVSGITALSPALTV
                     ASQTDDILYSVYDDGIYSIYRINAGRDAAGTPVGPDAVDRSSAILPPKARAGSELTGL
                     LRNSLFGLPMDDSAFTSAPYESKITLDYITPPQVGIGMDRFGSYAGGGIAMTFSDMLG
                     QHNVTGIAQLSNRLIDSAVIGLYTNSKSRTNFGFLGQRMPYSYGRYWIDYGDIGGEPA
                     YFINELIYRQIYYQLGGFATYPFSPAQRVEFQGGYSYIQFSNSLYRTGYSYYTGRRLS
                     FEDFDLPAADGLHMPFVSAALVYDTSLFGATAPIIGQSYRFEVSPTFWTLNYTTLLAD
                     FRKYVVPIKPFTLAFRVLHYGRYGAGSADDRLYPMYLGYGTLVRGYEYNSFTAEEVDM
                     KSGTFDYDRLFGSRIAVANLELRFPLFGVLGLGKGYYGVFPIDFIAFFDSAVAWDKEH
                     KAWFLGGDRKPLSSYGIGLRANIFGYFIVGCDY"
BASE COUNT          702 a          987 c         1104 g          671 t
ORIGIN      
        1 gtaatcgcag ccgacgatga aatatccaaa gatgttcgcc cgcaagccga tcccgtagct
       61 ggacaggggt ttgcggtcgc cgccgaggaa ccaggccttg tgctccttgt cccaagccac
      121 ggcgctgtcg aaaaaggcga tgaaatcaat ggggaagact ccatagtacc ccttgcccag
      181 ccccagaacg ccgaacagcg ggaaacgaag ctcgaggttg gccacggcga tgcgcgaccc
      241 gaacagccgg tcgtagtcga acgtcccgct tttcatatcg acttcctcgg ccgtgaagct
      301 gttgtactcg tatccccgga caagcgtccc gtacccgaga tacatcgggt agaggcggtc
      361 gtcggcgctg ccggcaccgt agcggccgta gtgaaggacc cggaaggcca gggtaaaggg
      421 cttgatggga acgacatact tgcggaaatc ggccagcaag gtcgtgtaat taagcgtcca
      481 gaacgtcggg gagacttcga aacgatagct ctggccgatg atcggcgcgg tcgccccgaa
      541 cagagacgtg tcatagacca gggcggcgct gacgaacggc atatggagcc cgtcggccgc
      601 cgggagatcg aaatcctcga aggagaggcg gcgcccggta taataggaat atcccgtccg
      661 gtacagcgag ttggagaact gaatgtagct gtagccgccc tggaattcga cccgctgggc
      721 cggactgaac gggtacgtgg cgaatccgcc caactgataa tagatctgcc ggtagatgag
      781 ctcattgatg aaataggccg gctccccgcc gatatcgccg taatcgatcc agtatctccc
      841 gtagctgtac ggcatccgct ggcccaggaa accgaaattc gtccggcttt tgctgttggt
      901 gtagaggccg atgacggccg agtcgatgag ccggttggac agctgggcga tgcccgtcac
      961 gttgtgctgg ccgagcatgt cgctgaaggt catggcgatg ccgccgccgg cgtaggagcc
     1021 gaaccggtcc atgccgatgc cgacctgggg cggcgtgatg taatccaggg tgatcttgga
     1081 ttcgtacggg gcggaagtga acgcggagtc atccatcggc agtccgaaca gcgagttgcg
     1141 gagaaggccc gtcagctccg agcccgcccg ggccttggga ggaaggatcg ccgaggagcg
     1201 gtcgacggcg tccgggccga ccggcgtccc ggccgcgtcc cggccggcgt tgatccggta
     1261 gatgctgtag atgccgtcgt cgtagacgct gtacaggatg tcgtcggttt gggaggcgac
     1321 cgtcagggcg gggctcaggg cggtgatgcc ggagacgccg gtatagagat tcgtaatctg
     1381 atagaaccgg cccgtgttcc ggtccaaccg gtagaggttg gagacgccgt cccggtcgga
     1441 gacgaaatac agctgccgcc cgtcggccga ccactgcggc gagatatgct tgccggcttg
     1501 aaaggccggc agctcccgga tctcccccgt ctcaggatcg aggagagcca ggtggaatcc
     1561 gcccgagccg accagggaga ggtcggaggc gaaccgctcg gtgatgaagg cgatccattt
     1621 tccgtcgggc gaccaggccg gttgaaggtc gccgtaggga tcggacgtca tgttaagaag
     1681 cgtttgggtt tccaggtcgt agatgaagag atccgaacat cccccggtca aggcggagaa
     1741 agcgatcttc cggccgtcgg gcgaccacgt cggattgaga atctcgccca aatggggaaa
     1801 gacgatgtcg tccttgagcc gccgcccccc gacgtcgaga aaagacaggg cgggtttgcc
     1861 ggcgatgacc gatccgaagg cgaattgcgt cccgtccgcg ctccaggacc cggctgaata
     1921 aataaactgg atgctctgga aatgggggtc gatcgaggtt ttcgtcagct tccgggtgat
     1981 ttttccggtg agggcgtccc ccaggaacat ctcgatcgag aacaggtcgc gcgaggagat
     2041 gaaaacgaaa tgcttcccgt cgggcgagat cgccggggcg caattcaggg acacatcctc
     2101 ggtccccttg accaccactt gggccttatc cagcgggccc tcggtggatt tttcgatcag
     2161 gccgtattcg gctttcaacg cttcgtgcca gtccttggag aattgctccg gagtgacgcc
     2221 caagacccgg gtgaaggctt tccggtagtc gtagccgcgg accacatccc gcatcagcct
     2281 ggcggccaca aggtcccccc accgccctcc aatataggca tagaccgctt ggccgtaccg
     2341 gtaggggaaa tacttgtagt aattttccag gtccttgagg gccgggatct ttttcctccg
     2401 gatcatatcc cgcatccaca tggccgtgtt gggatcctcc gggccgatcg aaaagtattc
     2461 ggccgcgcct tcgatcagga acagcggggt tcgggccagt ccctccccgc cctgcggatt
     2521 ggcccggcct tcccctccta aggccatgtc gtactggaag gcgtggacga gctcgtggcc
     2581 gatgacgtga tccgtgtcgt ccagggaagc tccgaagggc aggatgatcc tccgcttgaa
     2641 cgattcggtc acgccgcccg tgccctcgct gatcagctcg gagaggacgg tcgtctgctc
     2701 gaattgggga tggctggcgt acatgatgag ggcttgcttg cccttgagat catggttgaa
     2761 gatccgggcc agccggttgt accagcgctc ggccatgcgg gcggccgtcc ggacggcggc
     2821 ttcctcctcc ggatagtagt aaatctgaaa atgatcggtg gtcaggacct ggtaatcgaa
     2881 tttctcgtat tgaaccttgt tccggccgaa atattgagcc gaagccgtga cggccgtcgc
     2941 tccgaaaatc agcaggaaaa cgatccggcg aaaacggacg cccgacggct tatgttctcc
     3001 ggttgaggat ttcatgggaa ctccttcttc gcgaccttct ttcgcgctat ttcatcttcg
     3061 cttacggcta acgaattgtc aaaccggttt tccggcctaa acgttgccct atggataagt
     3121 tatcaagaat catgccaagc ccggtcccgg cgggaaaccg aagaaaaacg gagccggacg
     3181 tcctcttcgg aggacaggct gtcatctgaa gagacttccg gaagtcgccg gcggcccgga
     3241 tttttcagga gccgccgagg gctccgggaa agcgccggcc tttttccgaa tcgccgggaa
     3301 ccggagccgg aggatgaact tgggaacgat ctggttcgat tatattcagc aggccgtcct
     3361 cccgaggaca ggcttgaggc tgttttttca attatttcgt ccccgcgtga ttttaacgaa
     3421 ccggcgcaat cgattttgag taaatccgcc ctgggcataa tcaa
//
LOCUS       DAOOXH010000158         3454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_32711_length_3454_cov_4.543101, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000158 DAOOXH010000000
VERSION     DAOOXH010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32711_length_3454_cov_4.543101"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PKV64_11375"
     CDS             complement(<1..986)
                     /locus_tag="PKV64_11375"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPN17716.1"
                     /translation="MTNHKFSNVGLRLPLLASLLALPLFFAFGCKRAEAPFRFIDRLT
                     EENVAASPLKTASPPADSKAPGREWPESMLTTAVVLDPSFGKNPWSLEKSLVLGPDVT
                     PVLLAPPPTEFRYRLRIPLRGVLRFGCGLYSRNKKEDAEDTVFEINLEDVPGPGRIFE
                     ESINLDGAQSLHRVVLREIDLSAWAGKTVTIVFQTRAAGFSGTKDVRAFSQENGLAFW
                     VDPLLIPRREAGKAADAPPNIILISIDTLRADHLGSYGYRLPTSPATERLAGDGVLFT
                     SCFSGAPYTLASHATMLTGLNPARHQALYMADGIPPAVPTIAEILRRAGYETA"
     gene            complement(1238..>3454)
                     /locus_tag="PKV64_11380"
     CDS             complement(1238..>3454)
                     /locus_tag="PKV64_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPN17717.1"
                     /translation="HVDDHALWIDPRDTAHFLIGGDGGIYETYDGGAKYRFVTNLPVP
                     QFYRVTADDSRPFYYIYGGTQDNNSMGGPSRNTSADGVTSDEWFFTVGGDGFVSQVDP
                     TDTNIVYSEWQYGNIVRFDRRSGESFSIKPQPAKGQPTFRWFWDTPFLLSPHSPTRLY
                     IAAERIFRSDDRGDSWREISGDLTAGIDRNSIPVMGKYWSVDAVAKDVSTSQYGLIVS
                     LAESPLKDGLLFAGTDDGLIQITEDGGGEWRKVDRFPGIPARTYVSDICPSMFDENVV
                     YAAFNNTLNDDFKPYLLKSADKGRTWIPIASGLPDNGGVHTIIQDHVNPDLLFCGTEF
                     GVFATVTGGKEWFALKTGLPAISVKDLAIQRRENDLVAGTFGRGIYVLDDYTPLRHIK
                     ADDVQNEFRLFPVKDAVSFIRTSVKYGQGATCFLAPNPEYGAVFTYFLKESPLTRKEI
                     RQKRDKGLFDKGERIPIPSLDEQRRESEEHKPYLRFIVADESGRPIRILRAAPAAGVN
                     RIAWDLSYPLPVPARESGSFDPFGKDPSGFMVMPGRYTVSAAKVVDSVVTPLGEPQSF
                     TVTPLAGVTLAAADRAGLVAFQNDVAELTRAAYGADEETGVLAARVLRIRRTAAGMPG
                     APDGLMPAIRLAEKTLDEIRLALDGYEAKASFEEVPPAPMPIRNRVEELIGIQFSTTS
                     SPGKTQVENYRAAREELETVIRKLETLKTVDIPALEKILEEHRAPWTPGRIVKIGD"
BASE COUNT          595 a         1032 c         1170 g          657 t
ORIGIN      
        1 gcggtttcgt atccggcccg gcggaggatt tcggcgatcg tcggaacggc gggcggtatc
       61 ccatcggcca tgtaaagagc ctggtggcgg gccgggttca aacccgtaag catcgtcgca
      121 tgagaggcca acgtatacgg cgccccgctg aaacaggagg tgaacaggac tccgtcgccg
      181 gccaagcgtt cggtcgccgg gctcgtcgga aggcgatagc cgtacgagcc gagatggtcg
      241 gcccgcaggg tgtcgatcga aatcaggatg atattgggcg gcgcgtccgc cgctttcccc
      301 gcttcccgtc tcgggatgag gaggggatcg acccagaacg ccaagccgtt ttcttgggaa
      361 aacgcccgga cgtctttggt cccggaaaat ccggccgccc gggtttgaaa gacgatggtg
      421 acggttttcc ccgcccaggc cgacaggtcg atttcgcgga ggacgacccg gtgaagactt
      481 tgggctccgt caagatttat ggattcttcg aaaatccggc ccggaccggg aacatcctcc
      541 aaattgattt cgaagaccgt atcttccgca tcttctttct tgttcctgga gtagagcccg
      601 catccgaacc ggagaacgcc ccggagcgga attctgaggc gataccggaa ttccgtcgga
      661 gggggggcta gcaggaccgg ggtaacgtcc gggccgagga cgagactttt ttccagggac
      721 cagggattct tcccgaagga tggatcgagg accacggcgg tggtcagcat ggattccggc
      781 cattcccggc cgggtgcctt cgaatcggcc gggggggagg ccgtcttcag cggcgaggcg
      841 gcgacgtttt cctcggtcag ccggtcgatg aaccggaacg gagcctccgc ccgtttgcag
      901 ccgaaggcga aaaaaagcgg aagggcaagc agcgacgcga ggaggggaag acgcaagcca
      961 acgttcgaaa atttatgatt cgtcatcccg gtcgtccttt tcaacctggt ctcgggccgg
     1021 ccctccattt ttgattcttg tcccgctcgt tcgaacaaac gaattctagc gaagaatcgc
     1081 ccgcgttttc aagtttataa accattccgc ccggcggggg aatctccttc ccgcgcttcg
     1141 tcgagcgggg atcatcgctc caaaagcctc cgcccgtatt ccgggggaac cgcccggagg
     1201 gcgattcccc cggattgtcc ggcgttgatc cgggccgtca atctccgatt tttacgatcc
     1261 gtcccggagt ccagggcgcc cggtgctcct ctaggatttt ttccagggcg ggaatgtcca
     1321 cggtcttgag cgtctcgagc tttcggatca cggtttccag ttcctcccgg gccgcccggt
     1381 agttttcgac ctgggttttg cccggcgagg aggtcgtgga gaactggatg ccgatcagtt
     1441 cctcaacgcg gttgcggatg ggcatcgggg ccggcggaac ctcctcgaag ctggccttgg
     1501 cttcgtagcc gtccagggcc aggcggatct catcgagcgt tttctcggcc aggcgaatcg
     1561 cgggcatcag gccgtcgggg gcgcccggca tcccggccgc ggtccggcgg atccggagga
     1621 cgcgggcggc caggaccccg gtttcctcgt ccgcgccgta agccgcgcgg gtcagctcgg
     1681 ccacgtcatt ctgaaaggcg acgagcccgg cccggtcggc ggcggcgagg gtaacgccgg
     1741 ccagaggagt gacggtgaag gactggggtt ctccgagcgg cgtgacgacg ctgtcgacga
     1801 ccttggccgc ggagaccgtg tagcgtccgg gcatgaccat gaagccggac ggatccttcc
     1861 cgaacggatc gaaagatccg ctttcccggg ccggtacggg cagggggtag ctgagatccc
     1921 aggcaatccg gttaacgccc gcggcgggag cggcccggag gatccggatg ggccgcccgg
     1981 attcgtcggc gacgatgaac cggaggtaag gcttgtgctc ctccgattcc cggcgctgtt
     2041 cgtccagcga ggggatggga atgcgctcgc ccttgtcaaa aagccccttg tcgcgcttct
     2101 gccggatctc cttccgggtc agcgggcttt ccttgaggaa atacgtgaaa accgctccgt
     2161 attccgggtt gggggcgagg aagcaggtcg ccccctggcc gtatttcacg gacgtccgga
     2221 tgaaggacac ggcgtccttg acggggaaga gccggaattc gttttgaacg tcgtccgcct
     2281 tgatgtgccg cagcggcgtg tagtcgtcca ggacgtagat gccccggccg aatgttccgg
     2341 ccaccaggtc gttttcccgc cgctggatgg ccagatcctt gacgctgatc gccggcaggc
     2401 ccgttttgag ggcgaaccat tcctttccgc cggtgaccgt ggcgaagacg ccgaactcgg
     2461 tcccgcagaa cagaaggtcg ggattgacgt gatcctggat gatcgtgtgc acgccgccgt
     2521 tgtccggcag gccggaggcg atcgggatcc aggtccggcc cttgtccgcg ctcttgagaa
     2581 gatagggctt aaaatcgtcg ttgagggtgt tgttgaaagc ggcgtagacg acgttttcgt
     2641 cgaacatcga agggcagatg tcgctcacgt acgtccgggc cgggatgccg gggaaccggt
     2701 cgactttccg ccattcgccc cctccgtcct cggtgatttg aatgaggccg tcgtccgtcc
     2761 cggcgaaaag cagcccgtcc ttgaggggcg actcggccag cgagacgatc agtccgtact
     2821 gggaggttga gacgtccttg gccacggcgt cgacgctcca gtacttgccc atgaccggga
     2881 tcgagttccg gtcgatcccg gcggtcaggt cgcccgagat ctcccgccag gagtcgcccc
     2941 ggtcgtcgga ccggaaaatc ctctcggccg cgatatagag gcgggtgggg gagtgaggcg
     3001 agaggaggaa gggcgtgtcc cagaaccagc ggaacgtcgg ctggccttta gccggctggg
     3061 gcttgatgga aaagctttcc ccgctccggc ggtcgaagcg gacgatgttg ccgtactgcc
     3121 attcggagta aacgatgttc gtgtccgtcg ggtcgacctg ggaaacgaag ccgtccccgc
     3181 cgaccgtgaa gaaccactcg tcgctcgtga cgccgtcggc gctggtgttc cgcgaggggc
     3241 cgcccatgct gttgttatcc tgggtcccgc cgtaaatata atagaagggc cgcgaatcgt
     3301 ccgcggtcac ccggtaaaac tgagggaccg ggaggttcgt cacgaaccgg tatttcgccc
     3361 cgccgtcgta ggtctcgtaa atccccccgt ccccgccgat gaggaagtgg gcggtgtccc
     3421 gcgggtcgat ccagagggcg tggtcgtcga cgtg
//
LOCUS       DAOOXH010000159         3433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_32978_length_3433_cov_7.486382, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000159 DAOOXH010000000
VERSION     DAOOXH010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32978_length_3433_cov_7.486382"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKV64_11385"
     CDS             <1..516
                     /locus_tag="PKV64_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPN17718.1"
                     /translation="LGLGTPLGVAGGLFHLFNHAIAKSLLFFTSGSVQQATGTRNLDE
                     MGGLAKRMPWTAGTNLVGSLSIAGVPPLGGFWSKLVIIMALVQAREWSLAVIAVLASV
                     LTLWYYLILQRKAFFGKLAERWAQVKEAPFWMTAATVILALLVIGVGLWFAAAFSSWV
                     QPAADALAKGF"
     gene            520..1005
                     /locus_tag="PKV64_11390"
     CDS             520..1005
                     /locus_tag="PKV64_11390"
                     /inference="COORDINATES: protein motif:HMM:NF024641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HPN17719.1"
                     /translation="MTFQIILLAALVLASAAAILAKDLLKSAVALAVASLFLGIIFFR
                     MGAPYAGVFEISVVAGLITVLFMLTIALTKQGGDVRESKTVKVVFPLFFAVFLLIDFF
                     VMRGILAQVPALEGAAEAGTFGDVLWKERSFDLIGQLAVILAGVFAVLALFRKRNHHD
                     H"
     gene            995..1306
                     /locus_tag="PKV64_11395"
     CDS             995..1306
                     /locus_tag="PKV64_11395"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HPN17720.1"
                     /translation="MIINEWVVYLLFAAVLFFLGLYCLLTMRNLIKLFIGIEVIGKGV
                     SLVLLSTGWARQSIFLAQSLVVTYIVIEVSLVATALAVIINLYRVNQTLDIRSLTRLK
                     G"
     gene            1313..1645
                     /locus_tag="PKV64_11400"
     CDS             1313..1645
                     /locus_tag="PKV64_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17721.1"
                     /translation="MNGIDILLSPPVAFVLILAVVVLIYLLGKRMAPKLTRTGGKLTS
                     YACGEDIPGTKVQFGYRLFFFVALFFTIMHVAALVIATVPAGKIALFAVLYLAVVFLS
                     ILALITRS"
     assembly_gap    1723..1822
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1823..2221
                     /locus_tag="PKV64_11405"
     CDS             <1823..2221
                     /locus_tag="PKV64_11405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HPN17722.1"
                     /translation="WSRKKSPWILHLNSGACNACDIEVVAALTPRFDVERLGVLLKAT
                     PRHADVIVATGPVTRQLRDRIIRIYDQTPDPKFVIAVGACAMSGCVYRGAYNICGGLD
                     QVIPVNVYIPGCPVRPDAILDGVVKLLNTL"
     gene            2232..2699
                     /locus_tag="PKV64_11410"
     CDS             2232..2699
                     /locus_tag="PKV64_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPN17723.1"
                     /translation="MNDEQKIEKLKTFVSPEAVAHPAPHRIFVTVAPSGLVTLVERMK
                     AELDNWYVITISGVDKGETYEILYHFGDTTGSITVRTQIPKADPRLPSICGPIPGAVL
                     YERELQDMFGIAVDHLPDPRPMLMPDDWPAGNFPLRKDWKFDRPAEVIPGGEK"
     gene            2696..>3433
                     /locus_tag="PKV64_11415"
     CDS             2696..>3433
                     /locus_tag="PKV64_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HPN17724.1"
                     /translation="MKFQIPIGPQHPALKEPISIRLTVEGEVIKDADLRLGYNHRGVE
                     KLAEEKSWVQNIYLCERICGICSHSHATCFVQGAEKLLEIEPPRRGVYLRYLVSELER
                     IHSHLLWLGVAGHEAGFDTFFMYTWRDREVVMDLLEMISGNRVHYAINTLGGVRRDID
                     ETLRGKILEGLAVLKARSEYYFKIGAAEPTFLARLAGVGPLTKQQAMDYCAVGPMARA
                     SGIDRDVRKNDPHDFYNEVEFDVATSDS"
BASE COUNT          573 a         1139 c          942 g          679 t
ORIGIN      
        1 ctcgggctgg gaacgcccct cggcgtggcc ggcggcctct tccatctctt caaccacgcc
       61 atcgccaaga gcctcctgtt cttcaccagc ggctcggttc aacaggcgac cgggacgcgg
      121 aacctggacg agatgggcgg gctggccaaa cggatgccct ggacggccgg gaccaacctc
      181 gtcggctcgc tctcgatcgc cggcgttccc ccgctggggg gcttttggtc caagctcgtc
      241 attatcatgg ccctggtcca agcgcgggaa tggtccctgg ccgtgatcgc ggtcctagcc
      301 agcgtgctca ccctctggta ttaccttatc cttcaacgca aagccttctt cggcaagctg
      361 gccgagcgct gggcgcaggt gaaggaagcc ccgttttgga tgacggcggc cacggtgatc
      421 ctagctctcc tggtcatcgg ggtgggcttg tggtttgccg ccgccttctc ctcctgggtc
      481 cagccggccg cggacgcgct ggcgaaggga ttttaagcca tgacctttca aatcatcctt
      541 ctcgcggcgc tggttctggc ctcggccgcg gccatcctgg ccaaggatct tctcaaatcc
      601 gccgtcgccc tggccgtcgc cagcctcttt ttgggaatca tttttttccg catgggagcc
      661 ccctacgccg gggtattcga gatctcggtc gtcgccgggc tgatcacagt cctcttcatg
      721 ctgacgatcg ccttgaccaa acagggcggg gacgtccgtg aatccaagac cgtgaaagtc
      781 gtctttccgc tcttcttcgc cgtctttctc ttgatcgact tctttgtcat gcgggggatc
      841 ctggcccagg tcccggccct ggaaggggcg gccgaggcgg gaactttcgg agacgttctc
      901 tggaaggagc gctcgttcga tctgatcggc cagttggccg tcatcctggc cggcgtcttc
      961 gccgtcctgg ccctcttccg gaaaaggaat caccatgatc attaatgagt gggtcgttta
     1021 cctcctgttt gccgccgtgc tgttctttct cggcctctac tgcctgctga cgatgcgcaa
     1081 cctgatcaag cttttcatcg ggatcgaagt catcggcaag ggcgtgagcc tggtcctcct
     1141 ctcgacgggc tgggccagac agagcatttt cctggcccag agcctggtcg tgacgtacat
     1201 cgtcatcgaa gtcagcttgg tggccacggc cctggcggtc atcatcaacc tgtaccgcgt
     1261 gaatcagacc ctcgatatcc ggtcgctgac ccggctgaaa ggttaatccg acatgaacgg
     1321 aatcgatatc ctcctctccc ccccggtggc cttcgtcctc atcctggccg tggtcgtcct
     1381 gatctatctg ctgggcaaac ggatggcccc caagctcacc cggaccggcg gcaagctgac
     1441 ctcctacgcc tgcggcgagg acatcccggg gacgaaagtc cagttcggct accggctctt
     1501 cttcttcgtc gccctgttct tcaccatcat gcacgtggcc gctctggtca tcgccacggt
     1561 tccggccgga aaaatcgcct tgttcgccgt cctttacctg gcggtcgtct tcctctccat
     1621 tctggccttg atcacaagga gctgacatgt tcgaacgcgt catccgctgg tcccggaaga
     1681 aatcgccgtg gatcctgcac ctcaactcgg gcgcctgcaa cgnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nntggtcccg gaagaaatcg ccgtggatcc tgcacctcaa
     1861 ctcgggcgcc tgcaacgcct gcgacatcga ggtcgtggcc gcgctcacgc cccgcttcga
     1921 tgtcgaacgg ctcggcgtcc ttctgaaagc cacgccccgc cacgccgacg tcatcgtcgc
     1981 caccggcccg gtcacccgcc agctgcggga ccggatcatt cggatctatg atcagacccc
     2041 cgacccgaag tttgtcatcg ccgtcggggc ctgtgccatg tccggctgcg tttaccgggg
     2101 ggcgtacaac atctgcggcg gcctggacca agtcattcca gtcaatgtct acatccccgg
     2161 gtgtccggtc cgccccgatg ccatcctgga cggagtcgtc aagctcctga ataccctctg
     2221 agagaacacc gatgaacgac gaacaaaaaa tcgagaaact gaaaaccttc gtctcccccg
     2281 aggccgtcgc ccacccggct ccccaccgga ttttcgtcac cgtcgcccct tccggccttg
     2341 tgaccctggt cgaacggatg aaagccgagt tggacaactg gtacgtcatc acgatttcgg
     2401 gcgtggacaa gggtgaaacc tacgaaattc tctatcattt cggggacacg accggctcga
     2461 tcacggtccg gacgcagatc cccaaggccg acccccgcct cccgtccatt tgcggcccga
     2521 ttccgggcgc cgtcctctac gagcgcgagc tgcaggacat gttcgggata gccgtcgatc
     2581 acctgcccga tccccggccg atgctcatgc cggacgactg gcccgccggg aacttcccgc
     2641 tgcgcaagga ttggaaattc gatcggccgg ccgaggtcat tcccggaggc gaaaaatgaa
     2701 attccaaatc cccatcggtc cccagcaccc ggccctcaag gagccgattt ccatccgcct
     2761 gaccgtcgaa ggcgaggtca tcaaggacgc tgacctccgt ctcggctaca atcaccgcgg
     2821 cgtggagaag ctggccgagg aaaaatcctg ggtccagaac atctacctgt gcgaacggat
     2881 ctgcgggatc tgctcgcatt cccacgccac ctgcttcgtc cagggggccg agaagcttct
     2941 cgaaatcgag ccgccccggc ggggcgtcta cctgcgctat ctcgtctccg agctcgagcg
     3001 gatccacagc catcttcttt ggctcggcgt ggccggccac gaagccggct tcgacacctt
     3061 cttcatgtac acctggcgcg accgggaggt cgtcatggac cttctggaga tgatttccgg
     3121 aaaccgcgtc cattacgcca tcaacacctt gggcggcgtc cgccgggaca tcgacgaaac
     3181 cctccgcggg aaaatcctcg agggcctcgc cgttctcaag gcccggagcg aatactactt
     3241 caagatcggc gccgccgaac cgaccttcct cgcccgtttg gccggcgtcg gccccctgac
     3301 gaaacagcaa gccatggatt attgcgccgt aggccccatg gcccgggcgt cggggatcga
     3361 ccgggacgtc cggaaaaacg acccgcacga cttctacaac gaggtcgagt tcgacgtcgc
     3421 cacctccgac tcc
//
LOCUS       DAOOXH010000160         3409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_33281_length_3409_cov_5.259988, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000160 DAOOXH010000000
VERSION     DAOOXH010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33281_length_3409_cov_5.259988"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PKV64_11420"
     CDS             <1..392
                     /locus_tag="PKV64_11420"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17725.1"
                     /translation="TREQLDRALLIQKENRKKRLGQILVELKYLTPDDICLALASQLG
                     CAWIDLSSLTIPEEVLGLLPRDVIQKYEVVPVESKFGELLIVASAQPRNEEMRRAVAE
                     GTTMRTEFVIAYDGHIAAALEKYYGFS"
     gene            555..1517
                     /locus_tag="PKV64_11425"
     CDS             555..1517
                     /locus_tag="PKV64_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HPN17726.1"
                     /translation="MIEEITAEALNPEAFIAENVERIRAVVGPDRAINALSGGVDSSV
                     VTMLGHRALGGRLRSVFIENGLMREREGASVVESFRALGVPVEIVDAREEFFAALKGL
                     TDPEEKREAITQTFYRRVFGRIVKESGAKVLLQGTILTDVDETVAGIKRQHNVFAQLG
                     IDPEATFGYRILEPLLPLRKDGVRIVGRALGLPDVLFNRKPFPGPALAARVIGEATPE
                     RIAVVRRATTILERRLEAGPAFQYMAVLHADRVPGIREGRRDFGLQIEVRCWDSVDAR
                     TASPTRLSWETLEAISGDIIREIPGVVSVTYHIAPKPPSTIEAV"
     gene            1547..1828
                     /locus_tag="PKV64_11430"
     CDS             1547..1828
                     /locus_tag="PKV64_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPN17727.1"
                     /translation="MPERLHAFILGNVQGVSFRWFVGEEAMRLGLRGWVRNRRDGRVE
                     VLAEGERPSLTVLLEGLRRGPRFARVEGVDIDWTEASGEFSDFRIVPTA"
     assembly_gap    1845..1944
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1945..2417)
                     /locus_tag="PKV64_11435"
     CDS             complement(<1945..2417)
                     /locus_tag="PKV64_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPN17728.1"
                     /translation="MNKTLIAYFSRTGNTKMVAEAIHRALGGNRKLAAIEDAGPLADY
                     GLIFAGFPVQAHSVPLPAERFLKSIPAGSNIALFSTHGAFPHHRLAQEALEYAAVLVS
                     QGRLLGTFHCRGKLSLQALEALAESPEHQEWTQMAPSAASHPDAHDLAEAAVFAR"
     gene            complement(2486..>3409)
                     /locus_tag="PKV64_11440"
     CDS             complement(2486..>3409)
                     /locus_tag="PKV64_11440"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPN17729.1"
                     /translation="RELFEFLVFIGIFFVGTVLLLARGIGAMIVTPVRRLLAGTREAA
                     LGNLEFEVTYRGRDEMKTLVDGFNGMIRSLKNHQRDMADLGRKAAWAEMARKVAHEVK
                     NPLTPIQLSAEHLLHVWQDRREDFEPALKESISYIVGEVENLRRIAQEFLDLSRAAAL
                     NKETVALDEILAETVDPYRKLLSDRLAFTAAIEPGLRFEGDRGKLKIAFRNLMINAIE
                     SIRRRGDIRIEARRAGDRFVIVIADTGAGMEPDVAEKIFEPYFSTKSAGTGLGLPITK
                     KIIEDHGGSIRIRSEARRGTEVILELPAGAP"
BASE COUNT          580 a         1038 c         1077 g          614 t
ORIGIN      
        1 tcacccggga acagcttgac cgggcccttc tcatccagaa ggagaaccgg aagaagcggc
       61 tcggccagat tctcgtcgag ctcaagtatc tcaccccgga cgacatctgc ctggccctgg
      121 cctcgcagct gggctgcgcc tggatcgacc tttcgagcct gacgatcccg gaggaggttc
      181 tcggccttct gcctcgcgac gtgatccaga aatacgaggt ggttcccgtg gagagcaaat
      241 tcggggagtt gctgatcgtg gcctcggccc aaccccggaa cgaggagatg cgccgggccg
      301 tggccgaagg gacgacgatg cggaccgaat tcgtcatcgc ctacgacggc catatcgccg
      361 ccgccctgga aaaatactac ggcttctcct gaggcgtttt tcctcctttt tccctgattc
      421 cttatccgcg cccttgaaat ccgaaaagaa cgaggggatc cgctcttctt gacacccgtc
      481 cccggcccgg ctatattgcg gacacgccgg agggaagagg agagggagag agtcgttttc
      541 tcaaggagtg tttcatgatc gaggaaatca ccgccgaggc attgaatccc gaagccttca
      601 tcgccgaaaa cgtcgagcgc attcgggcgg tcgtgggccc ggaccgggcg atcaacgcct
      661 tgtcgggggg agtcgactcc tccgtggtga cgatgctcgg ccaccgggcg cttggcggcc
      721 gtctccggag cgtcttcatt gaaaacggtc tgatgcgcga gagggaaggg gcgtcggttg
      781 ttgaatcatt ccgggccctc ggcgtcccgg tggagatcgt cgacgcccgg gaggaatttt
      841 tcgccgccct gaagggcctg accgatcccg aggaaaagcg cgaggcgatc acccagacct
      901 tctaccggcg ggtcttcggc cggatcgtaa aggaaagcgg agccaaggtt ttgcttcaag
      961 gaaccatcct gaccgatgtc gacgagacgg tcgccgggat caagcgccag cataacgtct
     1021 tcgcccagct gggcatcgat ccggaggcga cgttcggata ccgcatcctg gagccgctcc
     1081 tgccgttgcg caaggacggc gttcggatcg tcggccgggc cctggggctc cccgacgtcc
     1141 tcttcaaccg gaagccgttt cccgggccgg ccttggccgc ccgggtcatc ggcgaggcga
     1201 cgcccgagcg catcgccgtc gtccggcggg cgacgacgat ccttgaacgc cgcttggagg
     1261 ccggccccgc ttttcaatac atggccgttc ttcacgccga ccgggttccg ggaatccggg
     1321 agggacgtcg cgatttcggc cttcagatcg aagtgcgctg ctgggacagc gtcgacgccc
     1381 ggacggcctc gccgacccgc ctttcctggg agacgctcga ggcgatttcc ggcgacatca
     1441 tccgggagat ccccggcgtc gtcagcgtca cctaccacat cgctcccaag ccgccctcga
     1501 cgatcgaggc ggtctgaggc cggcgggaaa gggggcctcc cgtcccatgc cggaacggct
     1561 gcacgccttc atcctcggaa acgttcaagg cgtttccttc cgctggtttg tgggggagga
     1621 agcgatgcgg ctcggattgc ggggctgggt ccgaaaccgg agagacggcc gggtggaagt
     1681 cctggccgag ggggagcggc cctccttgac agtccttctc gaggggcttc ggaggggccc
     1741 ccgcttcgcc cgggtggagg gcgtcgacat cgattggacc gaggcgtcgg gggaattctc
     1801 cgattttcgg atcgttccaa ccgcttgagc ccggctcagc ttttnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnntgccgg gcgaacacgg ccgcttcggc cagatcgtgg
     1981 gcgtcgggat gggaggcggc cgagggggcc atttgcgtcc attcctggtg ctcgggagat
     2041 tccgcgagcg cctccaaggc ctgaagcgac agctttcccc ggcaatgaaa cgtccctaaa
     2101 aggcggccct ggctcaccaa gacggccgcg tactccaggg cttcctgggc cagccggtga
     2161 tgggggaagg ccccatgggt gctgaacaaa gcgatattcg acccggcggg aatggacttc
     2221 aagaaccgtt ccgccggcaa gggaacgctg tgggcttgca cgggaaagcc ggcgaaaatc
     2281 aagccataat cggccagcgg gccggcatcc tcgatcgccg ccagcttccg gtttccgccg
     2341 agggcccggt ggatcgcctc ggcgaccatc ttcgtgtttc cagtccggct gaaatacgcg
     2401 atcagcgtct tgttcatggc ccttattttt tcatagacgg ggcgaaatat ccagagaaaa
     2461 cgggatcccg gcgtctccgg ccggttcagg gcgctccggc cggaagctcg aggatgactt
     2521 cggttccccg ccgcgcttcg ctccggatcc ggatcgaacc cccgtggtcc tcgatgatct
     2581 tcttggtgat gggaagaccc aggcccgtgc cggcggattt ggtcgaaaaa tacggctcga
     2641 aaatcttttc ggcgacgtcg ggctccatcc ccgcgccggt atcggcgatg acgatgacga
     2701 accggtcgcc cgcccgccgg gcctcgatcc gaatgtcccc ccgccggcgg atggattcga
     2761 tggcgttgat catgagattg cggaaggcga tcttgagctt cccccggtcc ccctcgaaac
     2821 ggaggccggg ctcgatggcg gccgtgaaag cgaggcggtc ggacaaaagc tttcggtagg
     2881 gatcgacggt ttcagccaga atctcgtcga gggccaccgt ttccttgttc aaagccgcgg
     2941 ccctcgacaa gtccaggaac tcctgggcga tgcggcggag gttctcgacc tcgccgacga
     3001 tgtacgatat cgattccttc agggccggct cgaaatcctc ccggcggtcc tgccagacat
     3061 ggagcaggtg ttcggccgaa agttgaatcg gggtcagggg atttttgact tcgtgggcga
     3121 ctttccgggc catttccgcc caagccgcct tgcggccgag atcggccatg tcgcgctggt
     3181 ggtttttcag gctgcggatc atgccgttga atccgtccac cagcgtcttc atttcgtccc
     3241 gtccccggta ggtgacctcg aattcgagat tgcccaaggc cgcctcccgc gttccggcca
     3301 gcagacggcg gacgggagtg acgatcatcg ctccgatccc ccgggcgagc agcaggacgg
     3361 tgccgacgaa gaaaatcccg atgaaaacga gaaattcgaa cagctcccg
//
LOCUS       DAOOXH010000161         3382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_33646_length_3382_cov_6.656147, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000161 DAOOXH010000000
VERSION     DAOOXH010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33646_length_3382_cov_6.656147"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PKV64_11445"
     CDS             <1..553
                     /locus_tag="PKV64_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HPN17730.1"
                     /translation="KEEWIKIRGESTPVKTLFLQYVIPLAAIPAAAQFIGYGLIGVRV
                     PFFGNLRLPFGTAIVRALLTFVLTAAAAYVAGLIINALAPSFASKQNMEAAMKLAVYA
                     LTPSWIAGVLYIIPALSILVGLAGLYGLYILYLGFQAPMMETPKEKVMSYFLVSLVVM
                     VVLWLVIGLIIGSVFALGAAPAL"
     gene            complement(548..1276)
                     /gene="pyrF"
                     /locus_tag="PKV64_11450"
     CDS             complement(548..1276)
                     /gene="pyrF"
                     /locus_tag="PKV64_11450"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPN17731.1"
                     /translation="MTPALAPSQRIITALDVENKAQALALVDQLGQASLFKIGLELFT
                     AEGPALLREIQALGKGIFLDLKLHDIPNTVGEAARIGVRNGARMMTIHASGGKSMMAR
                     AAETAAEEADKRGFPRPVLLGVTILTSLQNEDLASVGMAPDTPAQVLRLAALARAAGL
                     SGVVCSAKEIEIVRKETGPEFLIVTPGIRPAGASAQDQKRVMTPAEAVARGSDYLVIG
                     RPITQAASPAAAFAAIVRELEAGL"
     gene            complement(1391..2308)
                     /locus_tag="PKV64_11455"
     CDS             complement(1391..2308)
                     /locus_tag="PKV64_11455"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPN17732.1"
                     /translation="MADLSTDLGFLRLRNPVLTASGTFGYGTEFLPFLDLAAVGGIVV
                     KGLYFGPREGNPPPRLVETASGLINAIGLQGVGVERFAADVLPELRRRDTAVIVNVCG
                     SGDEEYYRVIEFLDGHEGIDAYELNISCPNVKRNGACPAMDPRATREIVAGAKRRSRR
                     PVIAKLSPNVTDIASIARAAEDGGADGISLINTLLAMAVDLDTRKPKLGNVLGGLSGP
                     AIKPVALRMTYQAARAVKIPVIGMGGICTGRDALEFMLVGARAVQVGTANFVDPASAV
                     RIVREIGDYCDARGLARAADIVGTLKTGL"
     gene            complement(2311..3102)
                     /locus_tag="PKV64_11460"
     CDS             complement(2311..3102)
                     /locus_tag="PKV64_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPN17733.1"
                     /translation="MRREPAARIIGKRSWGDFHLLRLESPDLAAGASPGRFLMIRTSD
                     GFDPLLRRPLSLHDAGDVWIELFFRVAGRGTALLAEKKEGETLDCLGPLGRGFRMDGF
                     GAGRTAWLIGGGRGVAPLYYLARTLISRGAGVRVFYGGKTSAEIPLREKFESAGIPLD
                     CATDDGSFGFAGFVTALLERALEGGRPDGLFACGPDAMMKRTGRMALGLGLPAQLSLE
                     AVMGCGFGACWGCVHRIKKDDGGQWRKICEDGPVFGADEILWEEA"
BASE COUNT          577 a         1081 c         1121 g          603 t
ORIGIN      
        1 caaggaggag tggattaaaa ttcggggcga atcgacgccc gtcaaaaccc tcttcctgca
       61 atatgtcatc cccctggccg cgattcccgc cgcagcccag tttatcggtt acggcctgat
      121 cggagtccga gtgccctttt tcgggaatct ccgccttccg ttcgggacgg cgatcgtccg
      181 ggctctcctg acgtttgttc tgaccgcggc ggctgcgtat gtggccgggt tgatcatcaa
      241 tgccctggcg ccgtcctttg cttcgaaaca gaacatggaa gcggcgatga agctcgccgt
      301 atatgccctg actccgtcct ggatcgcggg cgtgttgtac atcatccccg cgctcagcat
      361 ccttgtcggc ctggccggtc tctacggctt gtatatcctt tacctcggat tccaagcgcc
      421 gatgatggag acgcccaaag aaaaagtcat gtcttatttt ctcgtcagct tggtggtcat
      481 ggtcgtcctc tggctcgtta tcggcctgat catcggcagc gtgttcgcct tgggggcggc
      541 cccggcttta taagccggct tcgagctcac ggacgatggc cgcgaacgcc gcggccggag
      601 aggccgcctg tgtgatcggc ctcccgatga caagataatc cgacccgcgg gccaccgctt
      661 cggccggcgt catcacccgt ttctgatcct gagcggaggc gccggccggg cggatgcccg
      721 gtgtgacgat cagaaattcc ggtccggttt ccttccggac gatctcgatt tccttggccg
      781 aacagacgac cccggagagc cccgcggccc tggccagggc ggccaggcgg agaacctggg
      841 ccggcgtgtc cggtgccatg ccgacggagg ccaggtcctc gttttgcagg ctggtcagaa
      901 tcgtcacgcc cagaagaacc ggccggggaa atccccgctt gtccgcttcc tcggccgccg
      961 tttccgcggc ccgggccatc atcgatttgc ctcccgaggc gtgaatcgtc atcatccggg
     1021 cgccgttgcg gacgccgatc cgggccgcct ccccgacggt gttggggatg tcgtgaagct
     1081 tgagatcgag aaagatgccc ttgcccagcg cctggatctc ccggagaaga gccggccctt
     1141 cggcggtgaa aagctccagt ccgatcttga acagggaggc ttgtcccaat tgatcaacga
     1201 gagccagggc ctgggccttg ttttccacgt ccagggcggt gatgatgcgt tgagacgggg
     1261 cgagggctgg ggtcatgggg aatctcctcg gagtcgtttt cgcccggcta ttatatccgt
     1321 tccgcgtccg gggaatccat cgggagatga attgcgccgg ggcggtcgga tttctgtgaa
     1381 aagaccgggc tcagaggccg gtcttgagcg tcccgacgat gtccgcggcc cgggccaggc
     1441 ctcgggcgtc gcaatagtcc ccgatttcgc ggacgatccg gacggcggac gccggatcga
     1501 cgaaattcgc cgttccgacc tggacggccc gggccccaac cagcatgaac tccagggcgt
     1561 cgcgcccggt gcagatgccg cccatcccga tgaccggtat ttttaccgcc ctggcggctt
     1621 gataggtcat gcggagggcg accggtttga tcgccggacc gctcagcccg ccgagaacgt
     1681 tgcccagctt cggcttccgg gtgtccaggt ccacggccat ggccaggagg gtgttgatca
     1741 gggagatgcc gtccgccccg ccgtcctcgg ccgcccgggc gatggaggcg atgtcggtta
     1801 cgttggggga cagcttggcg atcaccggcc ggcgactgcg ccgcttggcc ccggcgacga
     1861 tctcgcgggt cgcccgggga tccatggccg gacaagcccc gttccgcttg acgttggggc
     1921 aggaaatgtt cagctcgtag gcgtcgatcc cctcgtggcc gtcgagaaac tcgatgaccc
     1981 ggtaatactc ctcgtcgccg gacccgcaga cattgacgat gaccgcggtg tcgcggcggc
     2041 gcagctcggg caggacgtcg gcggcgaacc gttcgactcc aacgccctgc aggccgatgg
     2101 cgttgatcag gcccgaagcg gtctcgacca gacgcggcgg gggattgcct tcccgcggcc
     2161 cgaaataaag ccccttgacg acgattccgc cgacggccgc aagatccagg aagggcagga
     2221 attccgtccc gtagccgaac gtccccgagg ccgtgagcac gggattccga agccggagaa
     2281 agccgaggtc ggtgctgaga tcggccatga tcaggcttcc tcccagagga tctcgtccgc
     2341 tccgaagacc gggccgtcct cgcagatctt gcgccattgt ccgccgtcat ccttcttgat
     2401 gcggtggacg cagccccagc aggccccgaa tccgcagccc atgaccgctt ccagcgacaa
     2461 ctgggcggga agaccgagcc cgagagccat ccgcccggtc cgcttcatca tcgcgtcggg
     2521 tccgcaggcg aacagcccgt cggggcggcc gccttcgaga gcccgctcca aaagcgcggt
     2581 gacgaagccg gcgaagccga acgatccgtc gtcggtcgcg cagtcgaggg ggattccggc
     2641 ggattcgaat ttctcccgga gggggatttc ggccgaagtt tttccgccgt agaagacccg
     2701 aacccccgct ccccgcgaaa tcaaggtccg ggccaggtaa tagagcgggg cgacgccccg
     2761 gcccccgccg atgagccagg cggttcggcc ggccccgaag ccgtccatcc ggaatccccg
     2821 tcccagggga cccaggcaat cgagggtttc gccttctttt ttttcggcca ggagggcggt
     2881 tccgcgtccg gcgacccgga aaaaaagctc gatccagacg tcgccggcgt cgtggaggct
     2941 gagcggccgg cggagaagag ggtcgaagcc gtcggaggtc cggatcatga ggaatcgccc
     3001 gggggaagcc ccggcggcta aatccgggga ttcgagacgc agaagatgaa aatcccccca
     3061 gctccgtttt ccgatgatgc gggccgcggg ttcacgcctc atgccggaga caaaatatca
     3121 gaacgtccgg gtgcggtcaa tcgccggttc ctcaggccgg ggatccccgg gcaggcgcgt
     3181 ccgcggatga attcatttcg gaatttcttt gggaggcggc cgcatgcccc tggaagccgg
     3241 ggccgggaga gggcgaagcc attggaaaag ggaggcggct ccgttttcgg agccggcttc
     3301 ccgagcagga gatgatcctc gggaacgcaa ggaacggaac ctcgtcgaaa ggaagaggac
     3361 cggtcgatca gcgcggatgg aa
//
LOCUS       DAOOXH010000162         3350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_34075_length_3350_cov_7.840668, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000162 DAOOXH010000000
VERSION     DAOOXH010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34075_length_3350_cov_7.840668"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1414
                     /gene="murC"
                     /locus_tag="PKV64_11465"
     CDS             23..1414
                     /gene="murC"
                     /locus_tag="PKV64_11465"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPN17734.1"
                     /translation="MVKTVYRKLKHIHLIGIGGTGMNGIAEVLHNLGYRVSGSDLNDS
                     DVTRRLRDLGATVSIGHRSENVHRADVVVISSAVREDNIEVREARRMKIPVIPRAEML
                     AELMRMRYGIAVAGSHGKTSTTSMIAAVLGKAGFDPTIVVGGRLNTVGAHARLGEGDF
                     FVAEADESDRSFLMLSPFIAVLTNIDAEHLDKYKGVDEIKKDFVTFANKVPFYCPVIL
                     SLDDANLQSIIPRLERRIITYGFSAQADFYARDMRYEAFSSVSTLLHKGREIGTLRLA
                     VPGPHMILNALAAVAVGRDLDIPPSVILDALAEYTGAGRRFELKADIREIMVIEDYGH
                     HPTEIRATLNGAKLGWPRRRLVAVFQPHRYSRLAHLRDEFATSFNQADALVLTEIYPA
                     GEDPIPGVSGRSLYEEMVRFGHKDVRFVPDFRDIPARLMELVRPGDIVIVLGAGNINR
                     IIPDLIRLLEKGT"
     gene            1411..2217
                     /locus_tag="PKV64_11470"
     CDS             1411..2217
                     /locus_tag="PKV64_11470"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPN17735.1"
                     /translation="MSREAGFVVTMQFQRNAEPARVKKPVRTSPLRLIHVLAAVAAVG
                     ALFFGVAQLHGFLLTWETLDVRSAEVVCPDPAVRELVRPIASRAAAGNILLLDASRVK
                     AGLEACPWVKEAHVRKVFPSSLAVDITPRRPAAILDAGRGFLLDRDNALIEPARPGDQ
                     ERLPLFHDDGMFAASRDAKLALAWACWDDLDAADREHVRRLDVTRPDDVVATFRDDPV
                     RLRLGAEGFARKIAEYRGARDRWTAAFGPLEYVDLRFPDRIYLKAAVEEE"
     gene            2220..>3350
                     /gene="ftsA"
                     /locus_tag="PKV64_11475"
     CDS             2220..>3350
                     /gene="ftsA"
                     /locus_tag="PKV64_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPN17736.1"
                     /translation="MAKNGYIVGIDIGTRTVTVLIGEINEERKIEVIGIGTAESRGLR
                     KGVVVNLDETSEAIKKARTDAELMAGQEIHAAFIGVSGAHIKSFNSRGVIAVSGRNRE
                     ITQEDIRRVLEQSKAVPIPPDREIIHVIPQEFIVDEQDGIHDPLGMNGIKLEVNVHIV
                     TGAVTLLQNLRTCIERSDIVIQSLVLNQIADAAAVLSPDEKELGVGLIDIGAGTTEVA
                     VFERGSLWYTSIIPLGGDNFTNDIAVGLRAAIPDAEKIKKKFGCALPPPGEEQETIEV
                     PVVGQNRKPRILSRQLLAEIIQPRAEEIFRLVDNDIKRMGFEKSLNAGLVLTGGTALL
                     DGLEVVAEEIFDLPVRRGDPGGVAGLMDRVRTPDFATPVGLLL"
BASE COUNT          600 a         1173 c         1030 g          547 t
ORIGIN      
        1 ccggaggaaa taggagaatc gcatggtgaa aaccgtctat cgcaagctca agcatatcca
       61 tctgatcggc atcggcggca caggcatgaa cggaatcgcc gaggttctcc acaacctggg
      121 ctaccgggtg tccggctccg acctcaacga cagcgacgtc acccggcgcc tgcgggacct
      181 cggcgcgacc gtttctatcg ggcatcggag cgaaaacgtc caccgggcgg acgtcgtggt
      241 catctcctca gccgtccggg aggacaacat cgaggtccgg gaagcccggc ggatgaaaat
      301 cccggtcatc ccccgggcgg agatgctggc cgaattgatg cggatgcgct acggcatcgc
      361 cgtggccggc tctcacggca agacgtcgac gacgtcgatg atcgccgccg tcctgggcaa
      421 ggccggcttc gacccgacga tcgtcgtcgg cggccggctg aacaccgtcg gagcccacgc
      481 ccggctggga gagggcgact tcttcgtggc cgaggccgac gaaagcgacc gctcgttctt
      541 gatgctctcg cccttcatcg ccgtcctgac caacatcgac gccgaacacc tggacaagta
      601 caagggcgtg gacgagatca aaaaggattt cgtcaccttc gccaataagg ttcctttcta
      661 ctgcccggtc attctcagcc tggacgacgc caatctccag tcgatcatcc cccgcctgga
      721 gcgccggatc atcacctacg gattctcggc ccaagcggat ttttacgccc gggacatgcg
      781 ctacgaggct ttctccagcg tctcgaccct cctccacaag gggcgggaga tcgggacgct
      841 ccggctggcc gttcccggcc cgcacatgat cctcaacgcc ctggccgccg tcgccgtcgg
      901 ccgcgacctg gacatcccgc cgtcggtcat cctcgacgcc ctggccgagt acacgggagc
      961 cggccggcgc ttcgagctca aggccgacat ccgcgaaatc atggtcatcg aggattacgg
     1021 ccaccatccc acggaaatcc gggcgaccct gaacggggcc aagctcggct ggccgcggcg
     1081 gcgcctcgtg gccgtcttcc agccccatcg ctattcccgg ctggctcatc tccgggacga
     1141 gttcgccacc tcgttcaacc aagccgacgc cctcgtcctg accgaaatct accccgccgg
     1201 cgaggatccg atccccggcg tgtcgggccg gagcctctac gaggagatgg tccggttcgg
     1261 ccataaggat gtccggttcg tccccgactt ccgcgatatt ccggcccggc tcatggagct
     1321 cgtccggccc ggcgacattg tcatcgtcct gggcgcgggc aacatcaacc ggatcatccc
     1381 cgatttgatc cgtctcctgg agaaaggaac atgagccgcg aagccggatt cgtcgtcacg
     1441 atgcagtttc agcggaacgc ggagcccgct cgagtcaaaa agcccgtccg cacgtcccct
     1501 ctccgtttga tccacgtctt ggccgccgtc gccgccgtgg gggctctctt tttcggagtc
     1561 gcccagcttc acggcttcct gctcacttgg gagacgctgg acgtccgctc ggccgaagtc
     1621 gtctgccccg acccggccgt gagggagctc gtccggccga tcgcctcccg ggcggccgcc
     1681 ggcaatattc tcttgctcga cgcgtcccgg gtgaaagccg gcctggaggc ttgtccctgg
     1741 gtcaaggaag cgcacgtccg caaagtgttc ccgtcgtcgc tggcggtcga tatcaccccc
     1801 cggagaccgg cggccatcct ggacgccggc cgcggcttcc ttctcgaccg cgacaacgcc
     1861 ttgatcgagc ccgctcgccc cggggaccag gagcgcctcc ccctcttcca tgacgacggg
     1921 atgttcgccg cgagccggga cgccaagctc gccctggcct gggcctgctg ggacgacttg
     1981 gacgcggcgg accgggagca cgtccgccgt ctcgacgtga cccgcccgga cgacgtcgtg
     2041 gcgacattcc gggacgatcc cgtcaggctc cgactgggcg cggagggatt cgcccggaaa
     2101 atcgctgaat accgcggggc ccgcgaccgt tggaccgccg ctttcggccc gctggaatac
     2161 gtcgaccttc ggttcccgga ccgcatttat ctaaaagccg ccgtcgagga ggaataatca
     2221 tggccaaaaa cggatatatc gtcggcatcg acatcgggac gcggacggtc accgtcctga
     2281 tcggagaaat caacgaggag cgcaagatcg aggtcatcgg catcggcacg gccgaatcgc
     2341 gcggcttgcg caagggcgtc gtcgtcaacc tcgacgaaac gtccgaggcg atcaagaaag
     2401 cccggacgga cgccgagctg atggccggcc aggagatcca cgcggcgttc atcggcgttt
     2461 cgggcgccca catcaagagc ttcaacagcc ggggcgtcat cgccgtctcc ggacggaacc
     2521 gggaaatcac ccaggaggac atccgccggg tgctcgagca gtcgaaagcc gttcccatcc
     2581 cccccgaccg ggagatcatc cacgtcattc cccaggagtt catcgtcgat gagcaggacg
     2641 ggattcacga cccgctgggc atgaacggca tcaagctcga ggtcaacgtc catatcgtca
     2701 cgggcgcggt gaccctgctc cagaacctcc ggacctgcat cgagcgatcc gatatcgtca
     2761 tccagagcct cgtcctgaac caaatcgccg acgccgcggc cgtcctctcc cccgacgaaa
     2821 aggagctcgg cgtcggcctc atcgacatcg gggcggggac gaccgaagtg gccgtcttcg
     2881 agcgcggaag cctctggtac acctcgatta tccccctggg cggagacaac ttcaccaacg
     2941 atatcgccgt cggcctccgg gcggccatcc ccgacgccga gaagatcaag aaaaaattcg
     3001 gctgcgccct tccgcccccg ggcgaggagc aggagacgat cgaggtcccg gtcgtcggcc
     3061 agaaccgcaa gccccggatt ctctcccgcc agcttctggc cgagatcatc cagccgcggg
     3121 ccgaggagat cttccgcctg gtggacaacg acatcaagcg catgggattc gaaaaatcgc
     3181 tcaacgccgg cctcgtcctg acgggcggga cggcccttct cgacggcctg gaggtcgtcg
     3241 ccgaggaaat cttcgacctg ccggtccggc ggggcgatcc gggcggcgtg gccggactca
     3301 tggaccgggt ccggacgccg gacttcgcca cgcccgtggg gctccttctc
//
LOCUS       DAOOXH010000163         3279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_35187_length_3279_cov_3.729839, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000163 DAOOXH010000000
VERSION     DAOOXH010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35187_length_3279_cov_3.729839"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(712..1041)
                     /locus_tag="PKV64_11480"
     CDS             complement(712..1041)
                     /locus_tag="PKV64_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HPN17737.1"
                     /translation="MKTILLLSISNIFMTFAWYGHLKFKNSALWKVVLISWSIAFFEY
                     CFQVPANRAGHGRFSAAQLKTIQEVITLVVFCVFSVAYLKEDLKWNYLVGFALILGAV
                     FFVFHKW"
     gene            1234..1653
                     /locus_tag="PKV64_11485"
     CDS             1234..1653
                     /locus_tag="PKV64_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17738.1"
                     /translation="MKKTVLSLAAALIALGLAACVTSQYSSSNLNYYRGQNVPPEFFK
                     VVSSTPTEITFEIKINFSQDRLYHIVLDGNTPLAEDWVLMVTGRGQAYTAVLKAKPGV
                     EFAAGKPYRLCIGDKNPEEVNVYSNNYRCLVDFDFTL"
     gene            complement(1668..>3279)
                     /locus_tag="PKV64_11490"
     CDS             complement(1668..>3279)
                     /locus_tag="PKV64_11490"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN17739.1"
                     /translation="LWSHLEDKGLRNKTFLVFAADHGESLGEHEEMSHGFFVYESAVH
                     VPLIFVTPFARLRGVVSPAVVSLTDVLPTVCEMSGLMPPTQIQGRSLVPFFFKPDRPS
                     DRLAYSETFYPRFHYGWSELQSFRDDRYKIILSPEPELYDLSLDPGETTNIIEKQRSV
                     FLALNDRAADFIKAAPQDSYKLDFSKVDEETQAKLAALGYIGSFSNPEKLKGKRLASP
                     RNKIGIFNELSRAREIGLEGDAQEAIGIFRRIIAEDPEITDAYFSLGNVYFKTGKYEE
                     AIQAFRQSLERKPDDSFTVINIANSYVAMGRFAEAERFVLEYIDSGFSDPQLYFLLGN
                     MNFHRKNYEAAVPYLKKCLSLNAQSASSHNTLAAIFIIKNDLGPAQEHLDAARRLNPR
                     LSNLNYNQAQLLENRGEVAAAVAAYEEELVQSPKHFKAMFNLSRLFRRTGEEGREREI
                     LRRCLEVNPDFPLAYFYLARIHLNRGENLEEAVRLVQRGIELKPEPQDLPLGYFLLAD
                     LYNRLGENGRSADAAQRGRELAARLPRQ"
BASE COUNT          697 a          916 c          995 g          671 t
ORIGIN      
        1 ttgggactgg tcaggaccag gctggccccc gggatcgcgt tgccttcctc gtccgcgatt
       61 ttaccgacga tggcgccggt gtcgcgggac tgggcgaaaa cgagaggaga gagcatcaac
      121 aacaggacga tcaatccaat gaaaagcttc gtcttcaaat gcgttcctcc tttttttaaa
      181 tttccctttt cggacaaagt ccgtcctttt cccttgggtg gttagtcatt ttcctggtga
      241 tcaccttcct tgttgtaccg atccgtttag cccatgaaat gagccttttc cctgaccata
      301 gaattgcaat gtttatgcca gtcgaataaa caccacgtat tttatttatt atcagatgat
      361 tatgaatctt tacacaattc caaatgtaaa aaaacacgga ataaaagact ttacatcttc
      421 tttccggccg ggcgttttcc acggattttc tccctccgct ctcccgttcc tcgacacccg
      481 cgattcgaca cgaggggagg gatcggcgca aacccgccca acggccaagt cccggtttct
      541 tttccagatc gccggacgtt cggcccgaac ggcggaacgg catccgcggc cggcggttcg
      601 gcgcgcgctt ctcgcgaagg attgtttttt cccgaaagga gacgccgtcc cgagttcgct
      661 tccgtcaaac gatccggccg gaaggcgaga acgcggcgcg ggacgcgccg ttcaccactt
      721 gtgaaagacg aaaaagaccg ccccgaggat caaggcgaat ccgaccaggt aattccactt
      781 caagtcttcc ttaaggtagg ccacggagaa aacgcagaag acgaccagag tgatgacctc
      841 ctggatcgtc ttgagctggg cggccgagaa ccggccgtgg ccggcgcggt tggccgggac
      901 ctggaagcaa tattcgaaaa aggcgatcga ccagctgatc aagacgactt tccagagggc
      961 ggagttcttg aatttcaggt ggccgtacca ggcgaacgtc ataaagatgt tggaaatgga
     1021 gaggagaaga atggttttca tgttcgtgaa gcaggggatt ttatgatttt ccccggaaaa
     1081 actcaatgac ggccccggga aaacgagatc atagcctccc ggctggaacg gtcaaggagc
     1141 gccgagcctc cggaccgata caaaggattg acttccgctt caacgaaagc cgataatgaa
     1201 aaccaaggga attcaacaac caaggagcgc acgatgaaaa agacggtcct gtccctggcg
     1261 gcggctctga tcgccctcgg cctcgccgcc tgcgtcacca gccagtattc ctcgagcaat
     1321 ctgaattact accgggggca aaacgtcccg cccgagtttt tcaaggtggt ctcctccacg
     1381 ccgacggaaa tcacgttcga gatcaagatc aacttttccc aggatcggct ctaccatatt
     1441 gttctcgacg gcaacacccc gctggccgag gattgggtgt tgatggtcac cggccggggt
     1501 caggcctaca ccgcggttct caaggccaag cccggagtgg aattcgccgc cggaaagcct
     1561 taccggctct gcatcgggga caagaatccc gaagaggtca acgtctattc caacaactac
     1621 cgctgcctgg tcgacttcga tttcaccctg taacggacgc cgggcggcta ttgccgggga
     1681 agccgggccg ccagctcccg gccccgttgg gcggcgtcgg ccgaacggcc gttctccccg
     1741 agccggttgt aaagatcggc caggaggaaa tagcccaggg gcaggtcctg cggctcgggc
     1801 ttgagctcga tccccctttg aacgaggcgg accgcttcct ccaggttctc gccccggttc
     1861 agatgaatgc gggccaggta gaaataggcc agcggaaaat caggattcac ctcgaggcag
     1921 cggcggagaa tctcgcgttc cctcccctcc tcccccgtcc gccggaagag gcgggacagg
     1981 ttgaacatcg ccttgaaatg cttgggggac tggacgagct cttcctcata ggcggcgacg
     2041 gccgccgcga cctctccccg gttttccaaa agctgggcct gattgtaatt gaggttgctc
     2101 aatcggggat tgagccggcg ggcggcgtcg aggtgctcct gggccgggcc gagatcgttc
     2161 ttgatgatga aaatcgcggc cagcgtgtta tgggaggacg cggattgggc gttgagggat
     2221 aggcacttct ttaagtaagg gacggccgct tcgtagtttt tgcggtggaa gttcatgttg
     2281 ccgaggagga aatagagttg gggatcgctg aagcctgaat cgatatactc caggacgaag
     2341 cgctccgcct cggcgaagcg ccccatggcc acgtaggaat tggcgatgtt gatgacggtg
     2401 aacgaatcgt ccggctttcg ctccagggat tgacggaagg cttggatcgc ctcctcgtat
     2461 ttcccggtct tgaagtagac gttcccgagg gagaaatagg catcggtgat ctccggatcc
     2521 tcggcgatga tccggcggaa gatcccgatg gcctcctggg cgtcgccctc cagcccgatc
     2581 tcccgggccc gggacagctc gttgaagatg ccgatcttat tgcggggact ggccaaccgc
     2641 ttccccttga gcttttcggg attggagaag gatccgatgt agccgagggc cgccagcttg
     2701 gcctgggttt cctcgtcgac cttgctgaaa tcgagcttgt agctgtcctg gggcgcagcc
     2761 ttgatgaaat ccgccgcccg gtcgttcagg gccaggaaga cggagcgctg cttctcaatg
     2821 atgttggtgg tttccccggg gtccagggaa aggtcgtaga gctcgggctc gggggacagg
     2881 atgatcttgt agcgatcatc ccggaagctt tgcagctcgg accagccgta atggaaacgg
     2941 ggatagaacg tttcggagta ggccaagcgg tccgaaggac ggtccggctt gaaaaagaac
     3001 ggaaccaagc tacggccctg gatctgggtt ggaggcatga ggccggacat ctcgcagacc
     3061 gtcggcagga cgtccgtcag ggaaacgacg gccggggaga cgacgccccg gagccgggcg
     3121 aacggggtca cgaagatgag ggggacgtgg acggccgatt cgtagacgaa aaacccgtgg
     3181 ctcatttcct cgtgttcgcc taggctttcc ccgtgatcgg ccgcgaacac gaggaaggtt
     3241 ttgttccgaa ggcccttgtc ctccaggtgc gaccacagg
//
LOCUS       DAOOXH010000164         3203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_36365_length_3203_cov_7.036213, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000164 DAOOXH010000000
VERSION     DAOOXH010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36365_length_3203_cov_7.036213"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1268
                     /gene="pgsW"
                     /locus_tag="PKV64_11495"
     CDS             135..1268
                     /gene="pgsW"
                     /locus_tag="PKV64_11495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HPN17740.1"
                     /translation="MRNETRLEPMPGKETKSRRRRAGFLTGLGLWSLAFGALFYFLPP
                     LPAPGTDEEARRAAALMARAVAALRERRESSGPPIDPLSDINRTGLIGREDTSITTSP
                     GRLEAKRTTANPLVAGALVRMFREAGAGRGDVAAVGASGSFPGLILATLCAAAARGIE
                     PRLIVSLGASRWGANDPGWTWLEMSDCLRRAGVLDVESAALSLGGGRDGGGGLPENGR
                     VLLEKKIEETGLPFIREPDLPKNVAARIALFEQSAGGRPIRVFVNIGGSFADMGTDGE
                     VLKLRPGFNPAGEIFVPPPDRRGVVQEMARRGIPVIHLLFVQGLAERYGLPWDPVPLP
                     GAGDGGSRPALSRPAGIVLLSLYLAGVAFGLFRIARLGRRLDD"
     gene            complement(1383..2726)
                     /locus_tag="PKV64_11500"
     CDS             complement(1383..2726)
                     /locus_tag="PKV64_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HPN17741.1"
                     /translation="MRAYESVALGIPEILLPQSGLDLTKWAVIACDQYTSEPAYWEKA
                     AGLVGDAPSTLHLIYPEVFLNEPEPEKNARIGRIRKTMTDYFARGLFTSEEGLVFVER
                     TTGGKTRKGLVAALDLEHYDYRKSSTTLIRATEGTIIERIPPRVRIREGAPMELPHIM
                     VLIDDPADEVIGPVAAAAGTLPKLYDFELMLGSGHLAGYRVAGRVLEDGILRGLSRLA
                     EPRAFAAKYGLKDGTPVLLYAMGDGNHSLATAKTIWEKTKEAAGGAAAVKDSPLRYAI
                     VELVNVHDEALAFEPIHRVLFDIASGRDLVAEMKAFYGGNVRSESLCCLNMMKAAVDS
                     QTGPAHRIGLITSEGFGVLEAEAAGSNLPVGTLQNFLDVFLKDKGAREIDYVHGTDVV
                     SELGAKPGNMGFYLAGMRKTDLFKTVILDGALPRKTFSMGEAWEKRFYMEGRKLI"
     gene            2896..>3203
                     /locus_tag="PKV64_11505"
     CDS             2896..>3203
                     /locus_tag="PKV64_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17742.1"
                     /translation="MTPRRSHGRLPGLVPFLAVLVFFVPACGKKTPAGKDEAKGISGL
                     VSRATEGRTDIDFQKGRITVHAPGGSAVLTSGGGSWPADLPEDLPPFRAGSIRNSTNS
                     "
BASE COUNT          522 a         1022 c         1084 g          575 t
ORIGIN      
        1 cggctgggtc attcccggca tcctggccaa caacctcgcc cggcaaaaaa tgatcagaac
       61 gctggccgcc ctggccgcgg cgacggtcgc gacctgggcc ctggccgagc tcatcgcctt
      121 tttctgaccg gatcatgagg aacgaaacgc ggctcgaacc gatgccggga aaagagacga
      181 aatcccgccg gaggcgggcg ggtttcttga ccgggctcgg cctctggagc ttggctttcg
      241 gagcgctttt ctatttcctg ccccctctcc ctgcgccggg aacggacgag gaagcccgcc
      301 gggcggccgc cttgatggcc cgggcggtcg ccgccctccg ggaacgccgg gagtcctccg
      361 gtccgcccat cgaccccctc tccgacatca accggacggg cttgatcggc cgggaggaca
      421 catcgatcac gacgtccccc ggacggctcg aagccaagcg gacgacggcg aatcccctcg
      481 tcgccggggc cctcgtccgg atgttccggg aagcgggcgc cggccggggg gacgtggccg
      541 ccgtcggcgc ctcgggatcg ttcccgggct tgattttggc gaccctctgc gcggccgcgg
      601 cccggggaat cgaaccgcgc ttgatcgtct ccttgggagc gtcccgctgg ggagccaacg
      661 atcccggctg gacctggctc gagatgtccg actgtctgcg ccgcgcgggc gtcctggacg
      721 tcgagtccgc ggccctgtcc ctcggcggcg ggcgcgacgg cggaggggga ctgcccgaga
      781 acgggcgcgt tcttctggag aaaaaaatcg aggagacggg cttgcccttc atccgggagc
      841 cggaccttcc taaaaacgtg gccgcccgga tcgctttgtt tgaacaaagc gccgggggac
      901 ggccgatccg ggtgttcgtc aacatcgggg gaagcttcgc cgatatgggg acggacgggg
      961 aagtcctcaa gctccggccc gggttcaacc cggccgggga gatcttcgtt cctccgccgg
     1021 atcggagggg cgtggtccag gagatggccc ggcgggggat tccggtgatt catctcctct
     1081 tcgtccaggg actggcggaa cgatacggcc ttccctggga tcccgttccg cttcccggcg
     1141 ccggagacgg cggatcccgg ccggcgctgt cccggccggc gggaatcgtc ctcctctctc
     1201 tttacctggc gggagtcgcc ttcgggctct tccggatcgc ccggctcggc cgccgcctcg
     1261 acgattgagc cggcggctcg gtcaaacgcc gctcattccg aacatcgccc ctcggggcgt
     1321 tcaccgagga cggtggaaaa cgatcgtttt cccgaaaaaa tctcaagtcg agggggcgga
     1381 gatcagatga gcttccgtcc ttccatgtag aagcgcttct cccaagcctc gcccatggaa
     1441 aacgttttgc gggggagagc gccgtcgagg atgacggttt tgaacaggtc ggttttacgc
     1501 atgccggcga ggtagaagcc catgttcccg ggcttggccc cgagttccga gacgacgtcc
     1561 gtgccgtgga cgtaatcgat ttcccgggcg cccttgtcct tgaggaaaac gtccaggaag
     1621 ttctggagcg tcccgaccgg aaggttcgag ccggccgcct cggcttccag gactccgaat
     1681 ccctcggagg tgataaggcc gatgcggtgg gccgggccgg tctgcgaatc cacggcggcc
     1741 ttcatcatgt tcaggcagca gagggattcg gaccggacgt tccccccgta gaacgccttc
     1801 atctcggcga cgaggtcgcg gccggacgcg atgtcgaata gaacccggtg gatgggctcg
     1861 aaagccaggg cttcgtcatg gacgttgacc agttcgacga tggcgtaacg aagcggggaa
     1921 tccttgacgg cggccgcgcc gccggctgct tcctttgtct tttcccagat ggttttggcc
     1981 gtggccagcg agtgattgcc gtcgcccatg gcgtagagca aaaccggcgt gccgtccttg
     2041 aggccgtact tggcggcgaa agcccgcggc tcggccagcc gggaaagtcc ccggaggatg
     2101 ccgtcctcga ggacccggcc ggcgactcgg tatccggcca gatgtccgga gccgagcatc
     2161 agctcgaaat catagagctt ggggagcgtt cccgcggcgg cggcgaccgg gccgatgact
     2221 tcgtcggccg ggtcgtcgat gaggaccatg atatggggaa gctccatggg cgcgccctcg
     2281 cggatgcgga cgcggggcgg aatgcgctcg atgatggtcc cctccgtcgc ccggatcaag
     2341 gtcgtcgagc ttttccggta atcgtagtgt tccaagtcca gggcggcgac caggcccttg
     2401 cgggtttttc cgcccgtcgt ccgttcgacg aagaccagcc cctcctccga ggtgaagagg
     2461 ccgcgggcga aataatcggt cattgttttc cggatccggc cgatccgggc gtttttctcc
     2521 ggttcgggct cgttcaggaa gacttcggga tagatcaggt gaagggtcga gggggcgtcc
     2581 cccacgaggc cggccgcttt ttcccaataa gccggctcgg acgtatactg gtcgcaggcg
     2641 atgaccgccc atttcgtcag gtcgagcccg gattggggaa gaagaatttc ggggatgccg
     2701 agggcgacgg attcgtaagc gcgcatggaa gctcctggtt tgtgatcgat ttccgcaagg
     2761 gaaagaggga atcttacccg agatcggccg cgttttcaaa ccccgggggc gtcgtcccgg
     2821 ccggatttcg tttttcccga tgatgggata gaatacggct tcgggagaga tcctcccgga
     2881 aaggaaaaga tccccatgac gccccgccgc tctcacggac gacttcccgg cctcgtcccg
     2941 ttcctggccg tcctggtctt tttcgtcccc gcctgcggca aaaagacgcc cgccggcaag
     3001 gacgaggcga agggaatttc cggcctggtc tcccgggcca ccgagggccg gaccgacatc
     3061 gacttccaaa agggacggat cacggtccat gcgcccgggg gaagcgccgt cctcacgtcc
     3121 ggaggaggat cctggccggc cgacctgccg gaagatcttc ccccgttccg ggcgggatcg
     3181 atccggaatt cgacgaattc cca
//
LOCUS       DAOOXH010000165         3202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_36386_length_3202_cov_5.516683, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000165 DAOOXH010000000
VERSION     DAOOXH010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36386_length_3202_cov_5.516683"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PKV64_11510"
     CDS             complement(<1..238)
                     /locus_tag="PKV64_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HPN17743.1"
                     /translation="MQKIVDAVYGYVWGYPLVILCLGAGIFFSIRTRFLQVRLIKDMV
                     GQLWRGTSSKKGVSSFQGFAMALGGRVGTGNIAGV"
     gene            451..2388
                     /locus_tag="PKV64_11515"
     CDS             451..2388
                     /locus_tag="PKV64_11515"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HPN17744.1"
                     /translation="MAACCAPACRSKSPAGPVVFRFIDGLTTAGVLESPLKNPEAAAL
                     GERAYPVNSAVMTEAGVGESAFGLKRKLALGLLSMDLLFAPPRSELRYRLPFKTEGIL
                     DFGIGIVHDVHSAARLAAAGSPSGEVKFQIILENAGRETILYERNLKQPEARTSRTIN
                     QIRERIPLPARTGEAGLRLVTTGPGGVFSYWIQPVLYVPGKKAPNIVLISIDTLRADH
                     VGAYGYERPLTPRIDALAADGVVFENAYSTSPWTLPAHISMLTGLNCVRHRVYAEQDR
                     MDPQTVTLAERLRARGYATWAITGGGFVSPIYGFAKGFDEYHLDQADLMDTSQAAKAG
                     YDAARWIEKNGDRPFFLFLHTYQVHSPYKSPMPYRTMFLRPDARWSRVDINKVVGGRA
                     GVYTELSEADRHNVIALYDAGVRFTDETLVQPVVDALRRLDLYDRTMIIVTSDHGEEF
                     FDHGGWNHSHSVYDELIRVPLVVKMPGSEFRGTRRSPVVRLTDLMPTILDYAGVPFQD
                     LSLNGESLRPVLKGKETSDRVFLAELADNILNCQIPQRLAMNDGRTKVILNRPFSPEN
                     MAFFTARPPAFPAVELYDLTRDPGEKTNILDHPDDPARIRSLVQKARDTDRLIPPRGE
                     GRHSRVDKSLEEQLRALGYIR"
     gene            complement(2428..2646)
                     /locus_tag="PKV64_11520"
     CDS             complement(2428..2646)
                     /locus_tag="PKV64_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17745.1"
                     /translation="MAKKMISIWFFIGLLLTVYGVLILGAGIYDLIVPGRHNVVLREL
                     HAGVWWGALLIVIGIVYLVFFRPKRTGK"
     gene            complement(2658..>3202)
                     /locus_tag="PKV64_11525"
     CDS             complement(2658..>3202)
                     /locus_tag="PKV64_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/galactose cotransporter"
                     /protein_id="HPN17746.1"
                     /translation="KTLSDGHFVSVGRWCTILGVLVSIGTAYLVMEFASIMDYVQALF
                     SFFIAPLFPTVLLGMLWKRTTSAGGFWGLLAGTVSSIGMWIWVKVDPSALRIIALSPG
                     AKAMAENMYRGLWSCLICAGVTVAVSLLTKPKPESALNGLVRSCTVLPSEKHLTLFRR
                     PAFWAGVVFVVFVFLNVLFW"
BASE COUNT          652 a         1100 c          932 g          518 t
ORIGIN      
        1 ccaccccggc gatgtttccc gtcccgaccc ggccgcccag ggccatggcg aagccttgaa
       61 aggacgacac gcctttcttg gaggacgtcc ctctccaaag ctggccgacc atgtccttga
      121 tcagccggac ctggagaaaa cgcgtccgga tcgaaaaaaa gattcccgct cccaagcaca
      181 ggatgaccaa gggatagccc cagacgtatc cgtaaaccgc gtcgacgatt ttctgcagat
      241 ccatgcgatc tctcctcggg cgggatgcaa tgaaaacaaa gaagccattc tatgtggaaa
      301 gaagagaacg gtcaaacgat ttttactcaa tacacagaat cggcccgtcg aataaggcgt
      361 atttggaccg aagggaaaat atgctatcat ctcccccctt gaaaccacat cgttcgcgcg
      421 gcctccgggg gacactcgtt cccgccctga ttggcggcgt gctgcgcgcc ggcttgccgc
      481 tcgaaatccc cggccgggcc cgtcgtcttc cgcttcatcg acggcctgac aaccgccgga
      541 gttctcgaaa gcccgctgaa aaacccggaa gccgcggctc tgggcgagcg ggcctacccg
      601 gtcaactcgg ccgtcatgac cgaagccggc gtcggcgaat ccgctttcgg cttgaagcgc
      661 aagctggccc tgggcctgct ctcgatggat ctccttttcg ccccgccccg gagcgaactc
      721 cgctaccggc ttcccttcaa gaccgagggc atcctggatt tcgggatcgg gatcgtccat
      781 gacgtccatt cggccgcccg gctcgccgcc gccggatccc caagcggcga agtcaagttc
      841 cagattatcc tcgaaaacgc cggccgcgag acgatcttgt acgaacgaaa cctcaaacag
      901 cccgaggccc ggacgtcccg gacgatcaac caaatccggg aacgcatccc tcttccggcc
      961 cggacgggcg aagccgggtt gaggctcgtc acgaccggac ccggaggcgt cttttcctac
     1021 tggatccaac ccgtcctgta cgtcccgggg aaaaaagctc cgaacatcgt cctcatctcc
     1081 atcgacacgc tccgggcgga tcacgtggga gcctatgggt atgaacgccc gctcacgccc
     1141 cggatcgacg ccctggcggc cgacggcgtc gtcttcgaaa acgcctattc cacctcgccc
     1201 tggacgctgc cggcccacat ctccatgctc accggcctca attgcgtcag gcatcgcgtc
     1261 tacgccgagc aagaccggat ggatccccag accgtcaccc tggccgagcg gcttcgggcc
     1321 cggggctacg cgacctgggc gatcaccggc ggcggcttcg tcagcccgat ctacggattc
     1381 gccaagggct tcgacgagta tcatctcgac caggccgacc tcatggacac gagtcaggcg
     1441 gccaaagccg gctatgacgc ggcccggtgg atcgaaaaaa acggcgaccg gccgttcttt
     1501 ctcttccttc acacctacca ggtccattcc ccctacaaaa gcccgatgcc gtaccggacg
     1561 atgttcctcc gcccggacgc ccgctggagc cgggtcgaca tcaacaaggt ggtcgggggg
     1621 agggcgggcg tttacacgga gctcagcgag gccgatcgcc acaacgtgat cgccctttat
     1681 gacgccgggg tccggttcac cgatgaaacc ctcgtccagc ccgtcgtgga cgccctccgc
     1741 cggctcgatc tctacgaccg gacgatgatc atcgtgacca gcgaccacgg cgaagagttt
     1801 ttcgatcacg ggggctggaa ccactcccac agcgtctacg acgagctgat ccgcgtgccc
     1861 cttgtcgtca agatgccggg ctcggaattc cgggggacgc gccggtcccc cgtcgtccgc
     1921 ttgaccgacc tgatgccgac gattctcgat tatgccggcg taccgttcca ggatctctcc
     1981 ctcaacggcg aaagcctccg gccggtcctg aaggggaaag agacgtccga ccgcgtcttc
     2041 ctggcggagc tggccgacaa catcctcaac tgccagatcc cccagcgcct ggccatgaac
     2101 gacgggcgga cgaaagtcat tctgaaccgc cccttctccc cggaaaacat ggccttcttc
     2161 acggcacgcc cgccggcctt tccggccgtc gagctttacg atctcacccg cgatcccggc
     2221 gaaaaaacaa acatcctcga ccatccggac gatcccgccc ggatccggag cttggtgcaa
     2281 aaagcccgcg acaccgaccg cctcattccc ccgcgcggcg agggccggca ttcgcgggtg
     2341 gacaagagcc tggaggaaca gctccgggcc ctgggataca tccggtaggg cggccgccgc
     2401 cggcgccgct ccggccgcgg cccgggatta cttgccggtc cgtttcggcc tgaagaaaac
     2461 gagatagacg atcccgatca cgatcagcag ggccccccac cataccccgg cgtgcaattc
     2521 acgcaggacg acgttgtggc ggcccggaac aatgagatcg tagatcccgg ccccgaggat
     2581 gagaacgccg taaacggtca gaagcagccc gatgaagaac cagatggaaa tcattttttt
     2641 cgccatgggc cggctcctta ccagaagagg acattaagga aaacaaagac gacaaagacg
     2701 acgccggccc agaaggccgg ccgccggaac aaggtcaagt gcttttccga gggcaggacc
     2761 gtgcagctgc ggaccagccc gttgagggcg ctctccggct tcggcttcgt gagcaggctg
     2821 accgcgaccg tgaccccggc gcagatcaag cagctccaca gcccccggta catgttctcg
     2881 gccatcgcct tggcgccggg cgacagggcg atgattcgga gggccgaggg atcgaccttg
     2941 acccagatcc acatcccgat cgacgagacc gttccggcca gcagccccca gaagccgccg
     3001 gccgaggtgg tccgcttcca gagcatgccg aggaggacgg tcgggaacag cggggcgatg
     3061 aagaaactga agagagcctg gacatagtcc atgatgctgg cgaattccat caccaggtag
     3121 gccgtgccga tgctgaccag gacgcccagg atggtgcacc accggcccac cgagacgaag
     3181 tggccgtcgc tcagcgtctt tt
//
LOCUS       DAOOXH010000166         3200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_36417_length_3200_cov_6.475676, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000166 DAOOXH010000000
VERSION     DAOOXH010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36417_length_3200_cov_6.475676"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PKV64_11530"
     CDS             complement(<1..1001)
                     /locus_tag="PKV64_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HPN17747.1"
                     /translation="MKFRFTLQRRLFLWFTLLFVVLTGAILFVIEKKEVATIYEESTN
                     KAVLIARHTADRNLQSILTWDMDRLQRDADRRRADAPLYVVFYDRYATPLVTSSLIAG
                     RPDLFQGSLLRERVDPEGFIAKSRDIVLFGRRLRILEVEVPIFAEGSPDKWGSVKIGL
                     SLEEMRAAVLRTRRTLILIGLTGLFLGLAGTTLLARRIVHPLRRLVEGTVRISRGEFG
                     HRIRHRPGDEIGDLAASFNEMTEKLLASRRETEEAQRLLVQSEKLAQIGRLAASIAHE
                     IRNPLTSVKLNIQKVLEDDAFPEADREHLALSGEGIAQIEKFIKEMLNFTRATELQKA
                     "
     gene            complement(1012..>1612)
                     /locus_tag="PKV64_11535"
     CDS             complement(1012..>1612)
                     /locus_tag="PKV64_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17748.1"
                     /translation="FIPDKLPGFLPSINPVGPAPDEAYRILEEEGYFHTKEFPEILLY
                     LPAGGKIDRAPFVRELTRQFETVGIRLKARDYWKLGDLADVRKPFFVLCQSSLDFPDP
                     ESVIQPLFGRSSPARWLTAGVPGPEFDVLLEEAAVESGRARYQDLFREMERMIRRDVP
                     GIPLFAAVQRLAVQPNVRGVRIPLLGTTYLDLRDVAFIR"
     assembly_gap    1613..1712
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1713..2419)
                     /locus_tag="PKV64_11540"
     CDS             complement(<1713..2419)
                     /locus_tag="PKV64_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPN17749.1"
                     /translation="MKFRKIIRIGISGILLSLFLSASSSSRDVYQPPDSQGDTFRIRN
                     FDTTFLSRFDPAKGESVFVSQQIFDGLVRLDNDLNIVPDLAEYWDISEGGRCYTFYLR
                     KGVRFHDGRELTAGDVEFSLRRLMDPETESPFGRLFVDKVVGGEEFAAGAAEDVAGFR
                     ILDRYIFELHWKKPYVSALGLLSMSFCRILPRETVEEDGSRFFARPVGTGPFKFDGWI
                     RSPRLDIVGVRLARNPS"
     gene            complement(2581..>3200)
                     /locus_tag="PKV64_11545"
     CDS             complement(2581..>3200)
                     /locus_tag="PKV64_11545"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HPN17750.1"
                     /translation="AECNRLGVMIDVSHLSDRSFAEVLNLSNAPVIATHSCCRTLCGS
                     PRNLTDDMIRALAEKGGVLQVCFVSSFLVDPPPNPERDAAFAALDAKSGSWSDMTPDQ
                     RTAAAAEFRALRRRFPEPPARLKDVVDHIDHAVKLAGIDHVGIGTDFDGGGEVEDCQD
                     ASRMYRVTMELLRRGYSEDQIAKIWGGNAFRVLQKVIDTASPSFL"
BASE COUNT          652 a          927 c          966 g          555 t
ORIGIN      
        1 gccttctgaa gctcggtcgc ccgggtgaag ttcagcatct ccttgatgaa tttctcgatc
       61 tgggcgatcc cctccccgct gagggcaagg tgctcccggt cggcttccgg gaaggcgtcg
      121 tcttcaagga ctttctggat gttgagcttg accgaggtca gcgggttgcg gatctcgtgg
      181 gcgatcgaag cagccaagcg gccgatttgg gccagcttct cggattggac caagaggcgc
      241 tgggcttcct cggtttcccg gcgggaggcc agcagctttt cggtcatctc gttgaacgaa
      301 gccgccaggt cgccgatttc atcgcccggc cggtgccgga tccggtggcc gaattccccc
      361 cgggaaatcc ggaccgtccc ctcgacgagc cggcggagag gatggacgat ccggcgggcc
      421 aggagcgtcg ttcccgcaag ccccaagaac aatccggtca acccgatcag gatcagcgtc
      481 cgcctcgtcc ggaggaccgc ggcccgcatc tcctcgagcg acaagccgat tttgaccgag
      541 ccccacttgt ccggagatcc ttcggcgaaa atcgggactt cgacttcaag aatcctcagg
      601 cggcggccga agagaacgat gtcccgggac ttcgcgatga agccctcggg atcgacccgc
      661 tcccggagaa gactgccttg aaaaaggtcc ggccggccgg cgatcaggga actggtgacc
      721 agcggcgtgg cgtaacggtc gtaaaaaacc acgtagagcg gcgcatccgc ccggcggcgg
      781 tcggcatcgc gctgcaaacg atccatgtcc caggtgagga tggactggag attccggtcg
      841 gccgtatggc gggcgatcaa gacggccttg ttcgtcgatt cctcgtaaat agtcgcgacc
      901 tctttctttt caatgacgaa gaggatggcc ccggtcaaga cgacgaagag gagagtgaac
      961 caaagaaaca accgccgctg aagagtgaag cgaaatttca tctcggcttt ctcatcggat
     1021 gaaggcgacg tcgcgcagat cgagataggt tgttccaagg agcggaatcc tcactccccg
     1081 gacattgggt tggacggcca gcctctggac cgccgcgaac agcgggatcc cgggaacatc
     1141 ccgccggatc atccgctcca tttcccggaa gaggtcctga taccgggccc ggccggactc
     1201 gacggccgcc tcctcgagaa ggacgtcgaa ttcgggaccg ggaacgccgg ccgtcagcca
     1261 gcgggcggga gaagaccggc cgaacagcgg ctggatgacg ctttccggat ccgggaaatc
     1321 caggctggat tggcaaagga cgaaaaaggg tttgcggacg tccgccagat cgcccagctt
     1381 ccaataatcc cgggctttca agcggatccc cacggtctcg aactgccggg tcagctctcg
     1441 gacgaagggc gcccggtcga ttttccctcc agccggcaaa tacaggagga tctccggaaa
     1501 ctccttcgtg tggaaatagc cctcctcctc gagaatccgg tacgcttcgt cgggagccgg
     1561 cccgaccgga ttgatggagg ggagaaatcc cggcagcttg tcggggatga aannnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntaggaggg attgcgggca agccggacgc
     1741 cgacgatgtc caggcggggg ctgcggatcc agccgtcgaa tttgaacggc cccgtcccga
     1801 ccgggcgggc gaaaaaccgg gaaccgtcct cttcgaccgt ttcccggggc aggatgcggc
     1861 agaagctcat gctcaggagc cccaaggccg agacatacgg ctttttccag tgcagctcga
     1921 agatgtaccg atccaggatc ctgaagcccg cgacgtcttc cgccgcgccg gcggcgaatt
     1981 cctcgcctcc gacgacctta tcgacgaaga gccgcccgaa cggcgattcg gtttcaggat
     2041 ccatcagccg gcggagggaa aactcgacgt cgccggccgt cagctcgcgc ccgtcatgga
     2101 accggacgcc cttgcgaaga tagaacgtgt agcagcgtcc tccctcggaa atatcccagt
     2161 actcggccaa atccgggacg atattgaggt cgttgtccag gcggacaagc ccgtcgaaga
     2221 tctgttggga gacgaaaacc gattccccct tggctggatc gaaccgggac aggaaggtgg
     2281 tgtcgaagtt gcggatgcga aacgtatcgc cttgggaatc aggcggttga taaacatctc
     2341 gactcgagga agacgcggag aggaacaggc tgaggaggat cccggagata ccgatccgga
     2401 tgatttttcg gaatttcata ccgctgaaaa acattatagc ccaagcctgc atccgcagca
     2461 aaaatgactt cttttaatag agcaatcttt atgccaacgc ccgctttaaa ataatgaaag
     2521 ggaaaacagg cttcacaaat cccaaaaccg ggtttatccg gctcccgttg taaattaatt
     2581 ttacaaaaac gacgggctgg ccgtgtcgat gactttttga agaactcgaa aagcgttccc
     2641 gccccagatt ttcgcgattt ggtcttcgga ataaccgcga cgcagaagct ccatcgtcac
     2701 ccggtacatt cggcttgcgt cttggcagtc ctcgacctct ccgcccccgt cgaaatccgt
     2761 tccgatcccg acgtggtcga tccccgccag cttgacggcg tgatcgatat gatcgacgac
     2821 gtccttcagc cgggccggcg gctcgggaaa tcggcgccgc agcgcccgga attcggccgc
     2881 cgccgccgtt cgctggtccg gcgtcatgtc ggaccaggac ccggacttgg cgtccagggc
     2941 ggcgaacgcc gcgtcccgtt ccgggttggg cggcggatcg acgagaaaac tcgaaacaaa
     3001 gcaaacttgg agaacgccgc ctttttcggc cagggcccgg atcatgtcgt cggtgaggtt
     3061 gcgggggctg ccgcagaggg tccggcaaca ggaatgggtg gcgatgaccg gcgcattcga
     3121 gagattcagg acttcggcga aggaacggtc cgacaggtgc gacacgtcga tcatgacgcc
     3181 cagccggttg cattcggcca
//
LOCUS       DAOOXH010000167         3196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_36493_length_3196_cov_6.273798, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000167 DAOOXH010000000
VERSION     DAOOXH010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36493_length_3196_cov_6.273798"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PKV64_11550"
     CDS             complement(<1..238)
                     /locus_tag="PKV64_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17751.1"
                     /translation="MISFGTSGWRAVMGEEFTFQNVRLVVQALANLLRAKYAGADVSV
                     LVNYDTRFLSERFAVEAARILSHNKIQVFLSERDA"
     gene            384..1007
                     /locus_tag="PKV64_11555"
     CDS             384..1007
                     /locus_tag="PKV64_11555"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPN17752.1"
                     /translation="MKKALSAAMILSFLGPLVVFAGPETSLESFSLAVSGGASYPLGS
                     RFQDTYFSGLNGGLTIGYRFSPAICAVLDIGYRRHGLDHGLFSSYYSVKGGDLGVFSI
                     VPGAKIYIPSRERFQVYLLAEAGLFLTAVSELTVSAPGFQGITRPKETESRFGYVLGA
                     GVEYFLMKKIGLWGDFRFTDVFPKSKDGEEKIHLRDYSFRLGARLLF"
     gene            1240..1758
                     /gene="coaD"
                     /locus_tag="PKV64_11560"
     CDS             1240..1758
                     /gene="coaD"
                     /locus_tag="PKV64_11560"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPN17753.1"
                     /translation="MGATDGEELAVYPGSFDPITNGHVDIIRRGAKLFPRILVAVLEN
                     PKKATLFSVEERMEIIREIFKDPPRIEVEAFHGLLADFTKLRGARAVIRGLRAVSDFE
                     YEMQMALMNQQLNPELETLFMMPSARYTFLSSSLVKEVFALGGSVRELVPSIVEQKLR
                     EKFAADPESLSI"
     gene            1774..2853
                     /gene="pilM"
                     /locus_tag="PKV64_11565"
     CDS             1774..2853
                     /gene="pilM"
                     /locus_tag="PKV64_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HPN17754.1"
                     /translation="MFGLTREKTVVGLDIGSHGVKILELNLKKKGKKDVFEVARIGYE
                     PLPHDVIVEGSIIDSAAVSATISEAFSKAKITSKDVALSISGNAVIIKKISLPAMETQ
                     ELAESIIWEAKHNIPYPYEETHVDYAILKPPPGSEDRGLEILLVAVKKEKVASLTNVI
                     NQAKKNLVAIEVDAFALFNAFEINYPEEFAEKSVAIIGLGANVTTIVVIDHGIPQLFR
                     DLSIGGTYITEAIRKDLNIGHQEAEALLRGVSPAGVSEADSATLLEMNIKELLDEIEK
                     TLAFYTAEDRREKKVDQIYLCGGLAGMGGLPAAFEHKFKVPTVPFNSFRKIHYNDKKL
                     DPAYDQDMAPLFSVAVGLATRKREK"
     gene            2858..>3196
                     /locus_tag="PKV64_11570"
     CDS             2858..>3196
                     /locus_tag="PKV64_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17755.1"
                     /translation="MIRVNLLKTDIKAGDKRPAVGVSVAAPVEIKPEKAKKSNAVNLI
                     IFLVIAVLGALAFLQKQSIDTERALLSEAQDQQRVLTPVLQKLELVEQQKFFLENKIR
                     LIEELRGRQAV"
BASE COUNT          692 a          962 c          914 g          628 t
ORIGIN      
        1 gcgcgtcgcg ctcggagaga aaaacctgga tcttgttgtg ggacaggatc cgggccgcct
       61 ccacggcgaa gcgttccgaa agaaaccggg tgtcgtaatt aaccaggacg gaaacgtccg
      121 ctccggcgta tttcgcccgg aggaggttgg ccagagcctg aacgaccagc cggacgttct
      181 gaaaggtgaa ttcctcgccc atcaccgccc gccagcccga cgtcccgaag ctgatcattt
      241 tttaactcaa cctagcacaa gatccgcctt cttggcaaat ccggccccct ccccggcggg
      301 gaccgaagcg cgaagaattg acttcttcgg ctaaaactcc tacgattcaa ccgattaacg
      361 agaataccgg gaggagggca gacgtgaaaa aagcgctgtc ggccgcgatg atcctgtcat
      421 ttctgggacc tctggttgtt ttcgccggac cggaaacctc ccttgagtca ttcagtctgg
      481 ccgtatccgg aggcgcctcc tatccgctgg gaagccgctt ccaggatacg tacttctccg
      541 gtttgaacgg cggactgacc atcgggtacc ggttctcccc ggcgatctgc gccgtcctcg
      601 acatcggtta ccgccgccac gggctcgatc acggcctctt ttcctcctat tattccgtca
      661 aggggggcga cctgggggtc ttctccatcg ttcccggggc caagatctat atccccagcc
      721 gggagcggtt ccaagtctat ctcctggccg aggccggcct gttcctgacc gcggtcagcg
      781 agctgaccgt ttcggccccg ggatttcaag gcatcacccg cccaaaggaa accgagtcga
      841 ggttcggcta tgttctcggg gccggcgtgg aatatttctt gatgaaaaag atcggtctct
      901 ggggagactt ccgattcacc gatgtttttc ctaagtcgaa agacggagag gagaaaatcc
      961 atctccggga ttactctttc cgcctgggag cgaggctgtt attctaagcg tctccggcct
     1021 ccctctcacc cccccgggag cccgctgcag gctatttatc ctgggattat tcacctgtcg
     1081 aatcacccct tggggggatt gtcccccggc gccggtcttg cataatgaac aggtctggga
     1141 taggattccc ggcagataac tgtaagaaag gagttcgtcc tgccgcccag gcggaaggat
     1201 tggatctgcc agaaatggaa ggagatgcgg agtaaggcca tgggagctac ggacggggaa
     1261 gaattggctg tataccccgg gtccttcgat ccgatcacca acggccacgt cgatatcatc
     1321 cgccgggggg cgaagctttt cccccgcatc ctggtggccg tccttgaaaa cccgaaaaaa
     1381 gcgaccttgt tttccgtcga ggaacggatg gagatcatcc gggaaatctt caaggatccg
     1441 ccccggatcg aggtcgaggc gtttcacggc cttctggccg atttcaccaa gctccgcggc
     1501 gcccgggcgg tcatccgggg gctgcgggcg gtttcagatt tcgagtatga gatgcagatg
     1561 gccctgatga accagcagtt gaatcccgaa ctggaaaccc tgtttatgat gcccagcgcc
     1621 cgctacacgt tcctcagctc cagcctggtc aaggaagtct tcgccttagg cggctccgtt
     1681 cgggagctcg tcccttccat cgtcgaacag aagctgcgtg agaaattcgc cgcggatccg
     1741 gagtctttat ctatataagg ggacaggtat gacatgttcg gcctaacccg agaaaaaaca
     1801 gtcgtcggac tggacatcgg ctcgcacggg gtgaagatcc tcgagctcaa cctgaaaaag
     1861 aaagggaaaa aggatgtctt cgaggtcgcc cgcatcggat acgagccgct ccctcacgac
     1921 gtgatcgtcg agggatcgat catcgactcg gcggccgtct cggcgacgat cagcgaggcc
     1981 ttcagcaagg cgaaaatcac ttccaaggac gtcgccctgt ccatttccgg caacgcggtt
     2041 atcatcaaga agatctcgct gccggccatg gagactcagg agctggccga atcgatcatc
     2101 tgggaagcca agcacaacat cccctacccc tacgaggaaa cgcacgtcga ttacgcgatt
     2161 ctcaagccgc cgccggggtc cgaggaccgg ggcttggaaa tccttctcgt cgccgtcaag
     2221 aaggaaaagg tggcctccct gaccaacgtc atcaaccaag ccaagaagaa cctggtggcg
     2281 atcgaggtcg acgccttcgc cctgttcaac gccttcgaga tcaattatcc cgaggaattc
     2341 gccgaaaaat ccgtcgccat catcggcctg ggggccaacg tcacgacgat cgtggtcatc
     2401 gatcacggca tcccccagct gttccgcgac ctgtccatcg gcggcacgta catcaccgag
     2461 gcgatccgca aggacctcaa cataggccat caggaggcgg aagcccttct ccggggcgtt
     2521 tccccggccg gggtctccga ggcggactcg gccaccctct tggagatgaa catcaaggag
     2581 cttctcgacg aaatcgagaa gaccctcgct ttttatacgg ccgaagaccg gcgggagaaa
     2641 aaagtcgacc agatttacct ctgcggcggc ctggccggga tgggcggcct gccggcggcg
     2701 ttcgagcata agttcaaagt cccgacggtt ccgttcaatt ccttccggaa aatccattat
     2761 aatgacaaaa aattggatcc ggcgtatgat caggacatgg ctccgctgtt cagcgtagcc
     2821 gtggggctgg cgacgcgcaa acgggagaaa tgagccgatg atccgcgtca atctcctgaa
     2881 gacggacatc aaagcgggcg acaagcggcc ggccgtcgga gtttccgtcg ccgcgccggt
     2941 cgaaatcaaa cccgagaaag cgaaaaagtc gaatgccgtc aatctgatca tcttcttggt
     3001 catcgccgtc cttggggcgc tggcatttct ccagaaacaa tccatcgaca ccgagcgggc
     3061 cctcctctcc gaggcccagg atcagcagcg cgttctgacg cccgtcctcc aaaagctgga
     3121 actcgtggag cagcagaaat tcttcctcga gaacaagatc cggctgatcg aggagcttcg
     3181 gggccggcaa gccgtc
//
LOCUS       DAOOXH010000168         3182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_36740_length_3182_cov_5.859290, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000168 DAOOXH010000000
VERSION     DAOOXH010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36740_length_3182_cov_5.859290"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..824
                     /locus_tag="PKV64_11575"
     CDS             315..824
                     /locus_tag="PKV64_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17756.1"
                     /translation="MKKTLTILSVVLLAAGGLFAQATKNLTMTVNLSAWYDLSLSPAS
                     ISFTDVAPTAADSPAAVSIKSNEGAVTVRAFAVTGSGKTLSLTVKANGDLTSGSNTIG
                     IGAISWASTGSGYVDGAMATSNVTAGSWSGSILHWHEGSFTYSFLRNYTTQAPGAYTA
                     SATYTLSAV"
     gene            953..1765
                     /locus_tag="PKV64_11580"
     CDS             953..1765
                     /locus_tag="PKV64_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17757.1"
                     /translation="MSGARIGFRDGKLFRLAAVLLAAALPAPGQIYFGISPIRAEHRI
                     KPGGSLTEVFLIRNNDAGPIRIKVYGENWTIRDDGTASFIGETPTTYSSKDWIVINPQ
                     DFRMNPGETKSVRYTIAVPPDTPPGGYHSAISFETVPDASAASSGNRMLFTGKIAAAV
                     YVIAGRPAVEGDLADFSLGEKNGQPAFLLSVENTGRTHFRTRGKIRAFSAAGEKLLDL
                     EIPDDVLLPESRKSVACPLPRPLDPGSYRVVCELDIGRPELMEMEKTIEVIQ"
     gene            1762..2286
                     /locus_tag="PKV64_11585"
     CDS             1762..2286
                     /locus_tag="PKV64_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17758.1"
                     /translation="MKKPIAAFALALALAGGALLAQTLPLTQTLNLTAFVNSRYKLEM
                     SAGSVTFTRVANPQTRPDIPQNESPISVTVKATTSRFLIFPQTINLRIEAVGTLTDPS
                     SGTTIPVSAVTWKASGVGFVGSGTLSSGTAALLGTWNASGVYDGTITFSFRDDPNYPP
                     GTYRLTVTLTVGAS"
     gene            2294..2773
                     /locus_tag="PKV64_11590"
     CDS             2294..2773
                     /locus_tag="PKV64_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17759.1"
                     /translation="MLKLVKSTIFPAAMSLFLAGAAAPLFGQNVELSLSRSSLSFAAA
                     DPDTTPLVPANDTLTVRIKVTGGGQPQPWQLTLRANGDLYSLLSLAMIDISNITWTAS
                     PAPPFQNGTLAANVDQVAASGSRNVNETGVMNFVFQNNWAYWAGSYTQSVTFTLSYR"
     gene            2775..>3182
                     /locus_tag="PKV64_11595"
     CDS             2775..>3182
                     /locus_tag="PKV64_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17760.1"
                     /translation="MNTLKTAAVLLCFAAAAPTLRGQMSLSLSPVHMEFILAAGNSEE
                     RMLTVGNDSPEDVRVRARIIAWAPGPAVPPDVPAAEGASLSSKDWLAVIAGDFRIPAR
                     QSLDIPVIAAVPQGAGPGQYPAVIAVQSVSDEAG"
BASE COUNT          604 a         1104 c          852 g          622 t
ORIGIN      
        1 cgggcccgaa atacgtccag cgcatcgttt acagcttgat gtcgacctga aatccgtctc
       61 tctcgtttat cactccactc agcccaagat tatatcgatt atccaattta taagtgacaa
      121 ttattgcttt ttgttgacaa tttacgtcat tatttttcaa taatactttg atcatattgc
      181 accctctttt tcgcatctta ttgtttttaa tatatttaaa ttattggcat ataacatgct
      241 ataattagag gtgtaacgaa acagattctt ttctcatagc gaggaagaga ttcgaaattc
      301 aacaggaggc tgacatgaaa aaaaccctga cgatcctgag cgtggtcctg ctggcggccg
      361 gcggtttatt cgcccaggcc acgaaaaacc tcaccatgac cgtgaacctc tcggcctggt
      421 acgacctgag cttgagcccg gcgtcgatct ctttcaccga cgtcgccccg acggccgcgg
      481 actcccccgc cgccgtttcg atcaaatcta acgagggcgc cgtcaccgtc cgggccttcg
      541 ccgtcaccgg aagtggaaaa acgctgagcc tgacggtcaa ggccaacggc gacctgactt
      601 ccggctccaa taccatcgga atcggcgcga tttcctgggc gtcgaccggc tccggttatg
      661 tcgacggcgc gatggccacg tccaacgtca cggccggctc gtggtcgggt tccatccttc
      721 actggcacga gggctccttc acctactcct tcctccgcaa ttacacgacc caggccccgg
      781 gcgcctacac cgcgtccgcc acgtacacgc tgtcggcggt ctgaccgggt ttaccaagat
      841 cgatgaaggc catgatccga gggcggggcg aatccccccg ccctcttttt ttgcccggca
      901 cggaacgatt gcctgggctc cgttttttaa atagaataga cgggggatga ggatgagcgg
      961 cgcaaggatc ggcttcaggg acggaaagct ctttcggctc gcggcggttc ttctggcggc
     1021 cgcgctcccc gccccaggcc aaatctattt cgggatctcc cccatccggg ccgagcaccg
     1081 gatcaagccg ggcggaagcc tgaccgaggt cttcctgatc cgcaacaacg acgccgggcc
     1141 gatccggatc aaggtctacg gagaaaactg gacgatccgg gacgacggaa cggcctcctt
     1201 catcggggag acccccacga catactccag caaggactgg atcgtcatca atccccagga
     1261 tttccggatg aacccgggcg agaccaaatc cgtccgctac acgatcgccg tacccccgga
     1321 cacgcctccg ggcggatatc actcggcgat ctcgttcgaa accgtgccgg acgcctcggc
     1381 cgcttcttcc ggcaaccgga tgctcttcac cggcaagatc gcggccgccg tctacgtgat
     1441 cgccggccgc ccggccgtcg agggcgacct ggccgatttc tccctcggcg aaaaaaacgg
     1501 ccagccggcc ttcctcctgt cggtggaaaa caccggccgg acgcacttcc ggacccgggg
     1561 gaagatccgg gcgttctccg ccgccggaga aaagctcctc gacctggaaa tccccgacga
     1621 cgtcctcctg cccgaaagcc gcaagagcgt cgcctgtccc cttccccggc cgctcgatcc
     1681 gggatcctac cgcgtcgtct gcgagctcga catcggccgg ccggagctca tggaaatgga
     1741 aaaaaccatc gaggtgatcc aatgaaaaaa ccgatcgccg ccttcgccct ggccctggcc
     1801 ctggccggcg gggcgcttct cgcccaaacg ctccctctga cccaaacctt gaatctcacg
     1861 gccttcgtca actcgcgcta caagctcgag atgagcgccg gctccgtgac gttcacccgg
     1921 gtcgccaatc cgcaaacgag gccggacatc cctcaaaatg aaagcccgat ttccgtcacc
     1981 gtcaaggcca cgacgtcccg ctttctcatc ttccctcaga cgatcaacct ccggatcgag
     2041 gccgtcggaa cgctcaccga tccctcgtcc gggaccacga tcccggtctc ggccgtcacc
     2101 tggaaggcga gcggtgtcgg attcgtcggt tcgggaaccc tctcatcggg gacggccgcc
     2161 ctcctaggga catggaacgc ctcgggcgtt tacgacggga cgatcacgtt ttccttccgg
     2221 gacgacccca attatccccc ggggacctac cgcctgaccg tgacgctcac cgtcggcgcg
     2281 agttgaggcc gtcatgttga agcttgtgaa atccacgatt tttccggccg cgatgtcgct
     2341 tttcctggcc ggcgcggccg ctccgctttt cggacagaac gtcgagctct ccctgagccg
     2401 ttcctcgctt tccttcgccg cggccgatcc cgacacgacg cccctcgtcc cggccaacga
     2461 cacgctcacg gtccgcatca aggtgacggg gggaggacag ccgcagcctt ggcagctcac
     2521 cctccgggcc aacggcgacc tctacagctt attgtccctc gccatgatcg acatttccaa
     2581 catcacctgg acggcctccc ccgcgccgcc gttccaaaac gggacgctgg cggccaacgt
     2641 cgaccaggtc gcggcttcgg gatcacgcaa cgtcaatgaa acgggagtca tgaatttcgt
     2701 cttccaaaac aactgggctt actgggccgg atcctacacc caatccgtga ctttcaccct
     2761 ctcctatcgg tgacatgaac accttgaaaa ccgccgccgt tctcctctgc ttcgccgccg
     2821 ccgcgcccac gctccgggga cagatgtcgc tgtcgctctc gcccgtccac atggaattca
     2881 tccttgccgc cggaaactca gaggagcgga tgctgaccgt cggcaatgat tcgccggaag
     2941 acgtccgggt ccgggcccgg atcatcgcct gggcccccgg tccggccgtt cctccggacg
     3001 tcccggcggc cgaaggcgcc tctttatcct ccaaggattg gctcgcggtc atcgccgggg
     3061 atttccggat cccggcccgt caatccttgg acatcccggt tatcgccgcc gtcccccaag
     3121 gcgccggacc gggacagtac cccgccgtga tcgccgtcca atccgtatcg gacgaggccg
     3181 gc
//
LOCUS       DAOOXH010000169         3172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_36900_length_3172_cov_6.479307, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000169 DAOOXH010000000
VERSION     DAOOXH010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36900_length_3172_cov_6.479307"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PKV64_11600"
     CDS             <1..695
                     /locus_tag="PKV64_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17761.1"
                     /translation="TIATYYNYEWMPRWIEEESYGKDVDYYVYRLAIPEVLPEFYKKI
                     FERIQRRGQYEVVEFASRKEIKPCVRPILSLMNDCYVNSNIYGYHPLDEKEMDDLAKR
                     YLPVLDPRFVKAVKKGPDFVAFIVGIPDMTEGIRKARGRLFPFGVFKILRAAKKTKQL
                     DLLLGGIREDCQGFGLDVFMGTRMIASAREAGLECIDTHHELESNVKVRAEMEKMGGV
                     VYKKFRVFTKNL"
     gene            complement(702..>3172)
                     /locus_tag="PKV64_11605"
     CDS             complement(702..>3172)
                     /locus_tag="PKV64_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HPN17762.1"
                     /translation="PYQGGFLIATDKGLSWAGENSVENRWAEADPLLAGDIRGAAVES
                     ASGEDVIWLLGARWLGIFQDGRLRILSRENPLLFSTNFSAATLLPDEKGGLFFGNPVS
                     IFYYDSSSARSYPLGVRNGLIAEGAAAFLLDRERNIWIGGFRGVSRISSRRFHNYRSP
                     QGLLEDEVTAIQAFGKGAVAFGHNFGLTIWEGRELRKIALQAKPDTPPGQIRVLDLAG
                     DDQGGLWVAAATLGLGRLEGKGGIRWHDVPGDPTHPVTSVVFDENRRLWTARSDALFQ
                     LRNGRQVEVFPGMFKGSYIRKIVAARGGLLFVATTKHGLFRWNGSSWTQAPGDEDSGA
                     SNVFSVYLDSNGRTLVGTLAGLRQFAGDRLDLFSENGFEIRRPVYLILEDRAGRLWFG
                     TDNGVVRWDGRVRRDFTKAEGLAGREVNRSAGMIDRLGRVWIGTNTGASSYRENFDLG
                     PDDVPPPTVSITGLDVNETPVDLSEEIILGYRENNLMFHFQGTSFIDESALRFESYLA
                     GFDKTWGSEYAAADRRIRYTNLPAGSYVFHLRAKNALGTRSATVSSPVIRIRNPFWLQ
                     WWFLLASFLLAGGLILSVGLAVTHHRQAERLEALVLDRTAQIQASLREKEILLKEVHH
                     RVKNNLQIISSLLFLQSRRITDPGLMSVFKGTISRVRAMALVHESLYRSDQLTLIDME
                     EYLRKLVYHLLEIYDVRKAPVAADIRTQGISLPLETAITFGLIVNELVSNALKHAFPS
                     ERKGVVKIILSRVGPDAAEEPAREKGIELTVADNGVGIPEAVKNGTPDSLGLRLVQNL
                     ASQLDGKIEVRNEEGTVVRIFFPE"
BASE COUNT          624 a          943 c         1001 g          604 t
ORIGIN      
        1 ccacgatcgc gacctattac aattacgaat ggatgccgcg gtggatcgag gaggaaagct
       61 acggaaagga cgtcgattat tatgtctacc ggctggcgat tcccgaagtc cttcccgaat
      121 tttataaaaa gatctttgaa cggatccagc gccgcggcca gtacgaggtc gtcgaattcg
      181 cctcacggaa agagatcaag ccctgcgtcc gcccgatcct ctctttaatg aacgactgtt
      241 atgtcaattc gaatatttac ggataccatc ccctcgacga gaaggagatg gacgacttgg
      301 cgaagagata tctgcccgtt ttggatcctc ggttcgtcaa ggccgtcaag aaagggccgg
      361 atttcgtggc gttcatcgtc ggcatccccg acatgaccga aggcatccgg aaagcccggg
      421 gccggctgtt tcccttcggc gtgttcaaaa tcctccgggc ggccaagaaa accaagcagc
      481 tggaccttct tctgggaggg atccgggaag actgccaggg gttcgggctg gacgttttca
      541 tgggcacgcg gatgatcgcg tcggcccggg aagcgggttt agagtgcatc gacacccatc
      601 acgagctcga gtccaacgtc aaggtccggg cggaaatgga aaaaatgggc ggcgtggttt
      661 ataagaagtt tcgggttttc acgaagaatc tctgagccgg cttattcggg gaaaaaaatc
      721 cggaccaccg tcccctcctc gttccggacc tcgattttac cgtccagctg cgaggccagg
      781 ttctggacga ggcgaaggcc gagggagtcc ggcgttccgt ttttaacggc ctccgggatc
      841 ccgacgccgt tgtcggcgac cgtcagctcg attcccttct cccgggccgg ctcctccgcc
      901 gcgtccggac cgacgcggct caagatgatt ttaacgaccc ccttccgctc ggagggaaag
      961 gcgtgcttaa gagcgttgga aaccagctcg ttgacgatca ggccgaaggt gatggcggtt
     1021 tccaggggca gggagatccc ctgggtccgg atgtccgcgg ccacgggggc tttccggacg
     1081 tcgtagattt ccagaaggtg atagaccagc ttccggagat actcctccat gtcgatcagc
     1141 gtcagctggt ccgaacggta caggctttcg tgaacgaggg ccatcgcccg gacccggctg
     1201 atggtcccct tgaacacgct catcagcccg gggtcggtga tgcggcggga ttggagaaac
     1261 agaaggctcg agatgatctg gagattgttt ttgacccggt ggtggacttc cttgaggagg
     1321 atttcctttt cccgcagaga agcctggatt tgggccgtcc ggtccaacac caaggcctcc
     1381 aggcgttcgg cctgccggtg atgggtgacg gccaagccga cgctgagaat caatccgccg
     1441 gcgagtaaaa aggaagccag caggaaccac cattgcagcc agaacggatt ccggatgcgg
     1501 atgaccgggg aggagaccgt cgcgctccgg gtccccaggg cgtttttggc ccgaagatgg
     1561 aagacatagg atcccgccgg aagattcgtg tagcggatcc gccggtcggc ggccgcgtat
     1621 tcgcttcccc atgttttatc gaatccggcc agatagcttt cgaaccgaag ggcgctctcg
     1681 tcgataaaag aggtcccctg gaagtggaac atgaggttgt tttcccggta tccgaggatg
     1741 atctcctcgg ataaatcgac cggcgtctcg ttgacgtcca ggccggtgat ggaaaccgtc
     1801 ggcgggggga cgtcgtccgg tccgaggtcg aaattctccc ggtagctcga ggcccctgtg
     1861 ttcgtcccga tccagacccg gcccaggcgg tcgatcatcc cggccgaccg gttgacttcc
     1921 cgtccggcga gcccctcggc tttcgtgaaa tcgcggcgca cccggccgtc ccaacggacg
     1981 acgccgttgt ccgttccgaa ccataggcgg ccggcccggt cttcgaggat gagatacacg
     2041 gggcggcgga tctcgaatcc gttttccgag aatagatcca gccggtcgcc ggcgaactgg
     2101 cgcaggccgg ccagcgttcc gaccagcgtc cggccgttgg agtcgagata gaccgaaaag
     2161 acgttggatg cgccggaatc ctcatctccc ggggcttgcg tccaggagct gccgttccag
     2221 cggaagaggc cgtgtttcgt cgtcgcgacg aagagcaacc cgccgcgggc ggcgacgatc
     2281 ttccggatgt aggagccctt gaacattccc gggaaaacct cgacttggcg gccgttgcga
     2341 agctggaaca gggcgtcgct tcgggccgtc caaagccgcc ggttttcatc gaagacgacg
     2401 gaagtgacgg gatgggttgg gtccccgggg acgtcgtgcc agcggatgcc tcccttccct
     2461 tccaagcgcc ccaagccgag ggttgcggcc gccacccaca gcccgccctg gtcgtctccg
     2521 gctaagtcca ggacgcggat ctggcccgga ggagtgtccg gtttcgcttg aagagcgatc
     2581 tttctcaatt cccggccttc ccaaatggtc aagccgaagt tgtggccgaa ggcgaccgcc
     2641 cccttgccga aggcctgaat ggccgtcacc tcgtcttcca ggagcccttg ggggctccgg
     2701 taattgtgaa agcggcggct ggaaatccgg ctcacgcctc ggaagccgcc gatccagatg
     2761 ttgcgttcgc ggtcgagcaa aaaagccgcc gcgccttcgg cgatgaggcc gttgcggacc
     2821 cccagcgggt aggagcgggc gctggatgaa tcataataga agatggagac cggatttccg
     2881 aaaaagagcc cgcccttttc gtcgggtaaa agcgtcgccg ccgagaaatt cgtggagaac
     2941 agcagaggat tctcccggct gaggatgcgg agccgcccgt cttgaaagat ccccagccag
     3001 cgcgcaccca ggagccagat gacgtcttct ccggaggcgg actcaacggc cgctcctcgg
     3061 atgtccccgg ccaatagggg atcggcctcg gcccaccggt tctcgacgga attttctccg
     3121 gcccaggaca accccttgtc cgtcgcgatg agaaaaccgc cctgatacgg ga
//
LOCUS       DAOOXH010000170         3167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_36978_length_3167_cov_7.054949, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000170 DAOOXH010000000
VERSION     DAOOXH010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36978_length_3167_cov_7.054949"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PKV64_11610"
     CDS             complement(<1..675)
                     /locus_tag="PKV64_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPN17763.1"
                     /translation="MPDLKTFRTAVDALRTKLDGECAGVRDADGLEDLRNRYLGRKRG
                     SVTLLFDQMKTLPAADKPAAGQMLNVLKTKVQEKLAALEESLRRPATRGPEIDPTLPG
                     ERLAWGAAHPLRIFQREIESIFEEMGFGIEDGPEIESDYYNFEALNFPPHHPARDDWD
                     TLYLGGDLLLRTHTSPVQIRVMEKRKPPIRIIIPGKVYRRETPDPTHAAMFFQVEGLV
                     VDRGITF"
     gene            complement(668..1024)
                     /gene="rplT"
                     /locus_tag="PKV64_11615"
     CDS             complement(668..1024)
                     /gene="rplT"
                     /locus_tag="PKV64_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPN17764.1"
                     /translation="MARVKRGVKRRIKREKVLGQAKGYWGAKSSNYRTAKEAVEKSLL
                     YAYRDRRAKKRDFRRLWIIRVKAGAMANGLTYSRFIQGLRKMGSPLDRKVLAEMAVNA
                     PEAFGRLVVQVKNALA"
     gene            complement(1035..1235)
                     /gene="rpmI"
                     /locus_tag="PKV64_11620"
     CDS             complement(1035..1235)
                     /gene="rpmI"
                     /locus_tag="PKV64_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPN17765.1"
                     /translation="MPKLKTHRGAKKRFKVTAAKKVLHKKANKSHILTKKKSNRKRNL
                     GQVALVDKTNRRAVKDMMPYEF"
     gene            complement(1261..1788)
                     /gene="infC"
                     /locus_tag="PKV64_11625"
                     /pseudo
     CDS             complement(1261..1788)
                     /gene="infC"
                     /locus_tag="PKV64_11625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
     gene            complement(1814..>3167)
                     /gene="thrS"
                     /locus_tag="PKV64_11630"
     CDS             complement(1814..>3167)
                     /gene="thrS"
                     /locus_tag="PKV64_11630"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPN17766.1"
                     /translation="LLSVSGAYWKGDERGIQLQRIYGTAFPTAEELEGYLRLLEEAKK
                     RDHRRLGQELDLFGTTEDLGGGLILWHPKGARIRAIIEQYWRERHWEGGYEVLYTPHI
                     GRENLWQTSGHLNFYKDSMYAPMDIDGQNYYLKPMNCPFHIQIYKSRMRSYRDLPLRW
                     AELGTVYRYERSGTLHGLLRVRGFTQDDAHIFCTPDQIDGEILRVLDFSLSILSDFGF
                     TDNKIELSVRDPKNAAKYCGEDDRWCRAEASLIKALEARGLPYERMEGEAVFYGPKID
                     IKIKDALGRHWQCTTIQFDFNMSERFDMAFIGEDGKEHRPFMVHRALMGSLERFFGIL
                     IEHYNGNFPLWLAPIQVEVIPIAERHNPYAEKLVAEIAARGLRARADLRREKLGYKIR
                     EAETHKIPLLLIAGDKEAAAGTASLRVHGQGDKGTVTLPEFLDKVKALSDRRSLAIAY
                     "
BASE COUNT          527 a          980 c          974 g          686 t
ORIGIN      
        1 gaaggtgatc ccccggtcta cgaccaagcc ctcgacctgg aagaacatcg ccgcgtgggt
       61 cggatcgggc gtctcccggc ggtagacttt ccccgggatg atgattcgga tcggcggctt
      121 gcgcttctcc atgacccgga tctgaacggg cgaggtgtgg gtccgcagca gcaagtcgcc
      181 gccgagataa agtgtgtccc agtcgtcccg ggcgggatgg tgcgggggga aattgagggc
      241 ttcgaaatta taataatcgg actcgatctc cggcccgtcc tcgattccga agcccatctc
      301 ttcaaagatc gactcgatct cccgctggaa aatccggagc ggatgggccg ccccccaagc
      361 cagccgctcc ccggggaggg ttggatcgat ctccgggccc cgcgtggccg gacggcggag
      421 actctcctcg agagccgcga gcttctcttg gactttggtt tttaggacgt tgagcatctg
      481 accggcggcc ggcttatcgg cggccgggag ggtcttcatc tgatcgaaga gaagggtgac
      541 cgagccccgc ttccggccga gataccggtt gcggagatcc tccaggccgt ccgcgtcgcg
      601 gactccggcg cactccccgt ccagcttcgt ccggagggcg tcgaccgccg tccggaatgt
      661 cttgaggtca ggcaagagcg tttttgacct ggacgacgag ccggccgaac gcttccggag
      721 cattgacggc catttccgcc aggaccttgc ggtccagggg gctgcccatc tttctcaagc
      781 cctggatgaa gcggctgtag gtcaagccgt tggccatcgc cccggccttg acccggatga
      841 tccagaggcg ccggaaatcc cgctttttcg cccgccggtc gcggtaggcg tacagcagcg
      901 acttctcgac ggcttccttg gccgtccggt agttcgagct tttcgcgccc cagtagcctt
      961 tcgcctggcc caggactttt tcccgtttga tccttctctt gactccgcgc ttgactctgg
     1021 ccatggatgt ctcctcagaa ctcgtagggc atcatatcct tgacggcccg acggttggtt
     1081 ttatcgacca gcgcaacctg accgagatta cgcttgcggt tcgatttttt ctttgtcagg
     1141 atgtggcttt tgttcgcttt tttgtgcaga actttctttg ccgcggtcac cttgaatctt
     1201 ttcttggctc cccggtgggt ttttagcttt ggcatctttt cctcctgtct tggcggggac
     1261 gagaaccgac gagacggccc agtccattct acggaatgcg ccgtccagct ttcccaagcc
     1321 ggcgcaatcg gcgacaaccc ggtccagcac cgccaggccc atttccggcc gggatttctc
     1381 ccggccgcgg agcatgatcg tgatcttgac cttgttcccc tcctcaagaa agcgctggac
     1441 gtgcttaagt ttgaagtcgt aatcatggat cgagatcttc ggccggaatt tgatttcctt
     1501 cagcaggacc tgcttctggt gcttcttggc ctcgtgatcg cgcttgtgca gctcgtacag
     1561 aaacttgccg taatccatga tccggcagac cgggggctgg gcttgcgggg cgatttcgac
     1621 gaggtccaga ccgcggttcc gggccagggc cagcgcctcg tgggcggcca tgacgccgag
     1681 cggctttttc tcctcatcga tcacccggat ttcccggcag gtgatcattt cgttcgtccg
     1741 atgaagtctc tgcttgacgg gcggaagcgt tgggtgtcgt cttaagatgg gcgtcctcct
     1801 tgatgttctc tcgttaatag gcgatcgcca gcgaccggcg gtcgctgagc gccttgacct
     1861 tgtccaggaa ctcgggaaga gtcacggtcc ccttgtcccc ctggccgtgg acccgaagcg
     1921 aagcggttcc agccgcggct tccttgtccc cggcgatcag gagaaggggg atcttgtgcg
     1981 tctcggcctc gcggatcttg tagccgagct tctcccgccg caggtcggcc cgggcccgaa
     2041 gaccccgggc cgcgatctcg gcgacgagct tttcggcata ggggttgtgc cgctcggcga
     2101 tgggaatgac ctcgacctga atcggggcca gccagagcgg gaaattcccg ttgtaatgct
     2161 cgatgagaat cccgaagaac cgctcgagcg aacccatcaa ggcccggtgg accatgaacg
     2221 gccggtgctc cttgccgtcc tcgccgatga acgccatgtc gaaccgttcc gacatgttga
     2281 agtcgaactg gatcgtggtg cactgccagt ggcggccgag cgcgtccttg atcttgatgt
     2341 cgatcttggg cccgtagaaa acggcctccc cctccatccg ctcgtacggc aggccgcggg
     2401 cttccaaagc cttgatcaac gaggcctcgg cccggcacca gcggtcatcc tcgccgcaat
     2461 acttcgccgc gttcttggga tcccggaccg aaagctcgat cttgttgtcg gtgaaaccaa
     2521 aatcggagag gatgctcagg gagaaatcca ggacccggag aatctcgccg tcgatttgat
     2581 cgggcgtgca gaagatgtgg gcatcgtcct gagtgaatcc gcggacgcgg agcaggccgt
     2641 ggagggttcc gctccgctcg taccggtaga cggtcccgag ctccgcccac cgcagaggca
     2701 ggtcgcgata ggagcgcatc cgggacttgt agatctggat atggaacggg cagttcatcg
     2761 gtttcagata gtaattctgc ccgtcgatgt ccatcggcgc gtacatgctg tccttgtaaa
     2821 aattcagatg gcccgaggtc tgccagaggt tttcgcggcc gatgtggggc gtatagagga
     2881 cttcgtagcc cccctcccag tggcgctccc gccagtattg ctcgatgatc gcccggatgc
     2941 gggcgccctt gggatgccag aggatcagcc ctccccccag gtcctcggtc gtgccgaaca
     3001 ggtccagctc ctggccgaga cggcggtggt cacgcttctt ggcctcttcc aggagacgaa
     3061 gataaccctc caattcctcg gccgtgggaa aggccgtgcc gtaaatccgc tgcagctgga
     3121 tgccccgctc gtcgcccttc cagtaagctc ccgagacgga gagaagc
//
LOCUS       DAOOXH010000171         3059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_38956_length_3059_cov_6.232357, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000171 DAOOXH010000000
VERSION     DAOOXH010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38956_length_3059_cov_6.232357"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PKV64_11635"
     CDS             complement(<1..629)
                     /locus_tag="PKV64_11635"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HPN17767.1"
                     /translation="MKKTCLAAATILLFALALPAAPGRVAKRFAVPSECATGLAFDGE
                     AFWTVDMETDKLYRLDPADGRVLAEFDAPGYFCTALAWDGRSLWAADMDFTNTSAEEY
                     CGKLYQLDPKTGRTLKVIMAPASDPQGLAWDGVSLWVSDNGTRELFQVSPDDGTTIKT
                     LKAPAGDPRGLAWDGRFLWLADRSEDELYRIDPESGRVLMILPAPGPHS"
     gene            1165..2541
                     /gene="mgtE"
                     /locus_tag="PKV64_11640"
     CDS             1165..2541
                     /gene="mgtE"
                     /locus_tag="PKV64_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPN17768.1"
                     /translation="MSGTKHARKGIAMVDVREPEIKKLIKKRDWDALRALAESWPMPE
                     LADLLADLPKPAGTILFRSLPKRIAAEVFSFLERSTRDAFLKELTDEETRTLLAGLSP
                     DDRTSFLEELPERDRQRLLNLLGPAERAEARTLLSYPEDSAGRLMTPEYVAVRPDWTI
                     GQTIAHVRRMGRDSETIDAIYVTDAAWKLLGVISLRQVVLAEPAETVAGLMITPAISV
                     SAFDDREAAALGMERYDLTVLPVVDSDGVLVGIVTADDVLDVAVEEATEDIQKAAAVA
                     PLEIGLKEASASLLYRKRILWLVALVFVNAVSGGIMARFETTIAALVPLVFFLPLLID
                     SGGNAGSQSATLMIRAMAVGEIRTNDWASLLLKEAGVSAALGLTTGLVAWGMGIWRGG
                     FALGLVVGLSMFLVVILGSLIGVCIPLLLKKLKLDPATASSPLITTVIDVAGVLLYFS
                     VASMLLDK"
     gene            2735..>3059
                     /locus_tag="PKV64_11645"
     CDS             2735..>3059
                     /locus_tag="PKV64_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17769.1"
                     /translation="MDKRSLLIFRAALLILSAGWAGGVLAGAADPAADVIFHRGTVLT
                     VDPFMTEAEAVAVKDGRILAVGSAADVLKYRGEATRMIDLDGRTLMPGFVEGHSHPFM
                     NMLVEN"
BASE COUNT          530 a          968 c          987 g          574 t
ORIGIN      
        1 caggaatgcg ggccgggcgc aggcaggatc atgaggacgc gtcccgattc gggatcgatc
       61 cggtaaagct cgtcctcgga gcggtcggcc agccaaagga agcgtccgtc ccaggccagc
      121 ccgcggggat cgcccgccgg cgccttgagg gtttttatcg ttgttccgtc gtcgggactc
      181 acctggaaca actcccgcgt tccgttgtcg gaaacccaga gggagacgcc gtcccaggcc
      241 aagccctgcg gatccgaagc cggggccatg atgactttga gcgtccggcc cgtcttggga
      301 tcaagctggt agagcttgcc gcagtactcc tcggccgagg tgttcgtgaa gtccatgtcg
      361 gccgcccaga ggcttcggcc gtcccaggcc agggcggtgc agaagtaacc cggggcgtcg
      421 aattcggcca gaacccggcc gtcggccggg tcgaggcggt atagcttatc cgtctccatg
      481 tccaccgtcc agaaggcctc gccgtcgaaa gccaggccgg tggcgcactc tgagggaacg
      541 gcgaagcgct tggccacccg gccgggagcg gccggcagag ccagagcgaa aaggagaatc
      601 gtcgcggccg ccaagcatgt ttttttcatc gtcgccctcc ttggttattt tgcccgcgtt
      661 cgaggcgcgg agaaaaacgg cgggcgccgg cggcggaatt ccgcggaagc gttcactccc
      721 gcccggaacg ccggcccttg tcccggcccg tctcaggggc aaggatagaa tccggccggg
      781 aggatgtcaa gcttgtcccc cagcccggaa tgatccgtcc cccttgaccg atctggccga
      841 agatgttctc ggggccggga ctttcggatc agctcgggag gcctgctttg ttttcggcgg
      901 ccttttcccc gcccgatgga atggaagccg gccttctttg cgtaacttgg attattcaca
      961 agccgagaca gccgctgccc ccccgccgaa aagaaaaatc caaaaccggg tttatttcaa
     1021 cacggcttcc ggaacgaggc gcgaaggggt gaatttgcgc cggccgcgcg tcgaattttt
     1081 cacggcgaag ccgatgcggc tatcatcatg caggtgaagt gcttttttca tccggaattg
     1141 ctattctgat gagggggacg ggacatgagc ggaacgaaac acgctcggaa agggatcgcc
     1201 atggtcgacg ttcgcgaacc ggaaattaaa aagctgatca aaaagcggga ctgggacgcc
     1261 ctccgggcgc tggcggaatc ctggccgatg cccgaattgg ccgatctcct ggccgatttg
     1321 cccaaacctg ccgggacgat cttgttccgc tctctcccca agagaatcgc ggccgaagtc
     1381 ttctcgttcc tcgagcggag cacccgggac gccttcctga aagagctgac cgacgaggaa
     1441 acccggaccc ttctggcggg tctcagtccg gacgaccgga cctccttcct ggaggagctg
     1501 cccgagcggg acaggcagcg cctgttgaac ctgctcggcc cggccgaacg ggccgaagcc
     1561 cggacgctgc tgagttatcc cgaagacagc gccggccggc tcatgacgcc ggagtacgtc
     1621 gccgtccgcc cggattggac catcggtcaa accatcgccc atgtccggcg catgggccgg
     1681 gattcggaaa ccatcgacgc gatctacgtg accgacgccg cctggaaact cctcggcgtg
     1741 atcagtctcc gccaggtggt tctggccgaa cccgccgaga ccgtcgccgg cttgatgatc
     1801 acgccggcga tttccgtctc ggcgtttgac gaccgggagg cggccgccct gggcatggag
     1861 cgttacgatc tgaccgtcct gccggtcgtc gattccgacg gtgttctcgt cgggatcgtc
     1921 acggccgacg acgtcctcga cgtagccgtg gaagaagcga ccgaggacat ccaaaaggcg
     1981 gccgccgtcg ccccgctcga gatcggtttg aaagaggcgt ccgcttccct cctgtaccgg
     2041 aaaaggatcc tctggctggt cgccctggtg ttcgtgaacg ccgtctccgg cgggatcatg
     2101 gcccggttcg agacgaccat cgccgccctc gtcccccttg tcttctttct tcctctcttg
     2161 atcgacagcg ggggcaacgc cggctcccag tcggcgaccc tcatgatccg ggccatggcc
     2221 gtcggggaaa tccggacgaa cgactgggct tcgctccttc tcaaggaggc cggcgtgtcg
     2281 gcggctttgg gcttgacgac gggactcgtc gcctggggca tggggatctg gaggggcgga
     2341 ttcgccttag gcctcgtcgt cggcctcagc atgttcctgg tcgtgatcct gggcagcctg
     2401 atcggagtct gcatcccgct gctgcttaaa aagctgaagc tggacccggc cacggccagc
     2461 tccccgctca tcaccacggt catcgacgtg gcgggcgttc tcctttattt ctcggtggcc
     2521 tccatgctcc tggataaatg agaggacggc cacggccgtc gcccccgggg gggcggtccg
     2581 acaccccgtc ccgccgcgtt ccttgacagt tcccggacgc tcctttattc ttcttctcct
     2641 tgttcatgaa gcgggccgcg atttcagtag acgacgggga tcgaaccgga gccggtcgac
     2701 aagggatttc cctgtgcgtc gaaaggaagg aaccatggac aaacggagtc tcctgatttt
     2761 ccgggcggct ctcctcatcc tctccgccgg atgggcgggg ggagttcttg ccggggcggc
     2821 cgaccccgcg gcggatgtca tcttccatcg gggaacggtg ctcacggtcg atccattcat
     2881 gaccgaggcg gaggccgtcg ccgtcaaaga cggccggatc ctcgcggtcg gatcggccgc
     2941 ggacgtcttg aaatatcggg gcgaggcgac aaggatgatc gacctggacg gcaggacgct
     3001 gatgccgggg ttcgtggaag ggcattcgca tccttttatg aatatgcttg tcgaaaaca
//
LOCUS       DAOOXH010000172         3021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_39696_length_3021_cov_4.179703, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000172 DAOOXH010000000
VERSION     DAOOXH010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39696_length_3021_cov_4.179703"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(378..896)
                     /locus_tag="PKV64_11650"
     CDS             complement(378..896)
                     /locus_tag="PKV64_11650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation endopeptidase HyCI"
                     /protein_id="HPN17770.1"
                     /translation="MNELSPLDWKAAVAAAMDTAGRVVVCGIGNDLRGDDAAGILCLQ
                     RLRRALSGRSAGSAPVRPVLFVDGGETPENETGKMRHFHPDLVLLIDAARGGRPSGEI
                     FTVDPESIADEEISTHRISLSLLVRYLEESVGARVLFLGIEPVSTSLHAPISDPVRRA
                     VDALAAFLEQTL"
     gene            complement(1022..1468)
                     /locus_tag="PKV64_11655"
     CDS             complement(1022..1468)
                     /locus_tag="PKV64_11655"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPN17771.1"
                     /translation="MKIAAFLPELFRHLFKKPATLDYPFKKIAVPKDFRGTPFLHPEL
                     CIVCKACERDCPAEAIEITSVNEAEKRFRMTIHNDRCIHCAQCVDSCPTTPVKAMEMD
                     TLHEIAAFDRHDLKKSWEYVRALPKRPVATPAPVKPAAETAVKSGA"
     gene            complement(1465..2436)
                     /locus_tag="PKV64_11660"
     CDS             complement(1465..2436)
                     /locus_tag="PKV64_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPN17772.1"
                     /translation="MAALKTILYLLVYPGFLFLFVYSTFVEWFDRKVYARLQNRMGPT
                     HTGRAGLLQPIADIVKLMAKEDIVPDKADKKVFTALPVVGLAVVLTAGLLLPVWNIDL
                     AAASPISFPGDLIVLLYLLSLPTLVLFLAGWSSTNAFSIIGGTRVLTMLFGYEVPLFL
                     AVLSPAVLAGSWRMAEIAAFYRAHPLLILPNLIGFGIALIAVQAKLERTPFDIPHAET
                     EIVGGTFTEYSGKKLAFFRLMADVELVVAAGLLATVFLGGFGGSVLPAFIQFVLKTLF
                     VVFLLSVLRAVTGRIRVDQVVSLAWKWLAPLAVLQLLIVILIKGFAL"
     gene            2627..>3021
                     /locus_tag="PKV64_11665"
     CDS             2627..>3021
                     /locus_tag="PKV64_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17773.1"
                     /translation="MFENLDRGRIVRQRRGPDLEALGFVRAVGDDVVDVFPARGLVAA
                     DDFARLGPAGRLDRDRPVRKLPEDLLADLVGFEKLLDPDDRPAEHVAGVGGGDVEFDL
                     VVEVVGVVFPDVPVDPRSPGHGADRAIIHG"
BASE COUNT          642 a          824 c          998 g          557 t
ORIGIN      
        1 agatcgggtt caacgcgcaa tatttcctcg attttctagg agccgttgaa acggataaaa
       61 tccgtttcga gctcaaggat gagaacagcg cggccctgct ccagccggac ggagagacag
      121 gcctggaaag caaatatgtc ttgatgccga tgaagatata acggactcgc cagggcagta
      181 aaacgagata tccaccgttt taggcatctt ttttcgataa aacaaatcct tatttgaaat
      241 catttctttt taatcactta agttaataag atatatcgca taaaaatagg gggctgtgga
      301 tatcgtgttg aaaattatga actttccgga ttcaggccgt tgagaggggc ccttttccgg
      361 cggccctggg agggagttta aagcgtttgc tctaaaaacg ccgccagcgc gtccacggcc
      421 cggcggaccg gatccgaaat cggggcgtgg agggacgtgg agacgggctc aatcccgaga
      481 aagagaacgc gcgccccgac gctttcctcc aggtagcgaa caaggaggga tagcgaaata
      541 cggtgagtgg agatttcttc gtcggcgatg gattccggat cgacggtgaa aatctctccg
      601 gagggccggc ctcctcgggc cgcgtcgata agaaggacca ggtccggatg aaaatgccgc
      661 atctttccgg tctcgttttc cggggtttct ccgccgtcga caaacagaac cggtcggaca
      721 ggagcggatc cggcggacct cccggacaga gccctccgca gcctctgaag gcagagaatc
      781 ccggcggcat cgtcgccccg gaggtcgttt ccgatgccgc agacaacgac tctcccggcc
      841 gtatccatcg ccgcggcgac ggcggctttc caatcgagcg gagagagttc gttcatgggg
      901 aatcctccct ggcatgataa ggcatttccc ggccttcggg caacgccggg cggtcgtcga
      961 taaaaaaaaa gccccgttcc ctggggaacg gggctctcag gatctcagcg cgagcggggg
     1021 attacgcccc tgatttgacc gcggtttccg ccgccggttt gacgggcgcg ggcgtggcga
     1081 ccggcctctt cggcagggcg cggacgtatt cccacgactt tttaagatca tgacggtcga
     1141 aggcggcgat ttcatgaaga gtgtccattt ccatcgcctt gaccggagtg gtcgggcagg
     1201 aatccacgca ctgggcgcag tggatgcacc ggtcgttgtg gatcgtcatc cggaagcgct
     1261 tttcggcctc gttgaccgaa gtgatttcga tcgcttcggc cggacagtcc cgttcgcagg
     1321 ctttgcaaac gatgcagagc tcggggtgga ggaacggcgt tccccggaaa tccttcggca
     1381 ccgcgatctt tttgaaggga tagtcgaggg tggccggttt tttgaaaaga tgacggaaaa
     1441 gctccggcag gaaggcggcg atcttcataa tgcaaaccct ttaatcagaa taacgatcag
     1501 aagctgtagg acggccagcg gggcgagcca cttccaggcg agggagacga cctggtcgac
     1561 gcggattctc cccgtcacgg ccctgaggac cgagaggaga aagacgacga agagcgtttt
     1621 caggacgaac tggatgaaag cgggcaggac gcttcccccg aagcctccga ggaaaacggt
     1681 cgccaacagg ccggcggcga caaccagctc gacgtcggcc atcagccgga agaaggccag
     1741 cttctttccc gagtattcgg tgaacgtccc gccgacgatc tccgtttcgg cgtgggggat
     1801 gtcgaagggc gtccgttcga gcttggcttg aacggcgatc agggcgatcc cgaagccgat
     1861 gaggttgggc aggatcagga ggggatgagc ccggtagaag gcggcgatct cggccatccg
     1921 ccaagacccg gccagcacgg ccggggagag gacggccaga aagagcggca cttcgtaacc
     1981 aaagagcatg gtcaggacgc gcgtcccgcc gatgatggaa aaagcgttgg tcgaggacca
     2041 gccggccaga aaaaggacca gggtcggcag actgagcagg tacagcagga cgatgaggtc
     2101 gcccgggaag gagatcgggc tggccgcggc caggtcgatg ttccaaacgg gcaggagaag
     2161 accggccgtg aggacgaccg ccaggccgac gaccggcagg gcggtgaaga ctttcttgtc
     2221 ggccttgtcc gggacgatgt cttccttggc catcagcttg acgatgtcgg cgatcggctg
     2281 gagaagaccg gcgcgcccgg tatgggtcgg ccccatccgg ttttggagcc gggcgtagac
     2341 tttccggtcg aaccattcga cgaacgtgga gtacacgaag aggaaaagaa aaccaggata
     2401 aaccaagagg tagagaatcg ttttcaaagc ggccatgcga cgttcctttg cagtgacagt
     2461 gaccgggcgc gatcgttcgc catccggcgc agatccgaga agcggagcgt gcgttcctct
     2521 ccggaccggg cgtcgaccag ctccactgac cgttcagcgc agcagatgca cgggtcagtg
     2581 gagctggtcg acgcccgaag atgaggggga cgtcggcgat gaagccgtgt ttgagaatct
     2641 cgatcgtggc cggatagttc gccagcgtcg gggcccggac cttgaggcgc tcgggtttgt
     2701 ccgagccgtt ggcgacgatg tagtggatgt tttccccgcg aggggcctcg tagcggctga
     2761 cgacttcgcc cggcttggcc cggcggggcg ccttgaccgc gatcggcccg tccggaagct
     2821 tccggaggat ctgctcgcag atcttgtagg attcgaaaag ctccttgatc cggacgatcg
     2881 tccggccgag cacgtcgcag gagtcggcgg tggcgacgtc gaattcgacc tcgttgtaga
     2941 agtcgtgggg gtcgtttttc cggacgtccc ggtcgatccc cgaagcccgg gccatggggc
     3001 cgaccgcgca ataatccatg g
//
LOCUS       DAOOXH010000173         3015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_39799_length_3015_cov_4.442905, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000173 DAOOXH010000000
VERSION     DAOOXH010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39799_length_3015_cov_4.442905"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="PKV64_11670"
     CDS             complement(<1..1539)
                     /locus_tag="PKV64_11670"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPN17774.1"
                     /translation="MMNKISPTFFVLSALILGPILAAAESVPIIELKENWAVRSSAGL
                     AAGGEDLSLPGAAFPDARPAMIPTTVLSALVKNGVYKDIFLADNFAKIPAEPFAVPWW
                     FRTEFDVPAVPGRGRAVLEFDGINYRADIWLNGKKIADTSQIYGAFRQFRLDVTDGIR
                     PGARNALAVMILPVEPGALTMGFVDWNPKAPDSNMGIWRPVRLRLTGDLVVHSPFVQT
                     RLDLKTLKEARLTVSAEIENKAGEPREGTLNGRIGDIRFSRPVALAAHEKKTVAVTPG
                     DCPQLVIRDPRVWWTHDLGTPELYTLNLEAALGGQVSDEASTRFGIREIADDVSPDGR
                     RGYVLNGRRILIRGGGWTDDILLDNPPDKVRAQVAYARHMNLNALRVEGFWGTGPEFY
                     DFCDETGILVMAGWSCQWEWENYFGKPTDEVYGGLLTAEDFDLAARSWRDQVRWLRNH
                     PSIFVWALASDLIPKPELEKSYRAILAEDDPTRPVLNSTGSKTSSVSGDSGVKMNGPY
                     DYVPP"
     gene            1697..3010
                     /locus_tag="PKV64_11675"
     CDS             1697..3010
                     /locus_tag="PKV64_11675"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HPN17775.1"
                     /translation="MEERIVLSSREIQRSLVLQKVLERGLTLKTAAKLMKLGYRQAKR
                     LLVRLRRDGPKGLAHRNRGRKAANAYDPKIRHKVLALSHGRYAQFNDTHFVEMLAERE
                     GLAISRETVRGWRREAGIKAKRQRRPPRHHKRRPRRPIMGLLAQWDGSPHPWFGSQHP
                     PCCLLHAVDDATNTVLGMLFRPQEDAIGYLRLLDMILRRHGIPVAIYQDRHAALFRTD
                     THWSHEEELAGIRFPTHVGRVLEEVGIEALAALSPQAKGRIERQGGTFQDRLVAELAL
                     AGITDIEIANTWLETTFIPRFNARFAIAPQQSGSAFRPIKAADRYRLVAFAYQAVVAN
                     DNTVRLGRLTIDIPPGSPRRSYAHQKVLVRQHLDGAWTVWQEDVQLARHAPTPLREPW
                     RGWKPREHGDDHRARHLIQIYLQTTPAPLEGDIFARQLGGHIDSA"
BASE COUNT          564 a          932 c          965 g          554 t
ORIGIN      
        1 gggggggaca tagtcgtaag ggccgttcat cttgacgccg gaatcgccgc tgacggagga
       61 ggttttgctc ccggtggaat tcaggaccgg ccgggtcgga tcgtcctcgg ccaggatggc
      121 ccggtaggat ttctcgagct ccggcttggg gatgaggtcg ctggccaggg cccagacgaa
      181 gatgctgggg tggttgcgga gccagcggac ctggtcccgc caggagcggg ccgccaggtc
      241 gaagtcctcg gcggtcagga ggccgccgta cacctcgtcg gtcggcttgc cgaaatagtt
      301 ctcccattcc cattggcagc tccagccggc catgacgagg attcccgtct cgtcgcaaaa
      361 gtcgtagaac tccggcccgg tgccccagaa accctccacc cggagggcgt tcaggttcat
      421 atgccgggcg taggcgacct gggcccggac cttgtccgga ggattgtcga gcaggatgtc
      481 gtcggtccag ccgccgccgc ggatcaggat cctccggccg ttgaggacgt agccgcggcg
      541 gccatccggg ctgacgtcat cggcgatctc gcggatgccg aaccgcgtcg acgcctcgtc
      601 ggagacctgt ccgccgaggg ccgcttccag gttcaaggtg tagagctcgg gcgtccccag
      661 atcgtgcgtc caccaaacgc ggggatcgcg gatcacgagc tggggacagt cccccggcgt
      721 caccgcgacc gtttttttct catgggcggc caaggcgacg gggcgggaaa agcggatgtc
      781 cccgatccgt ccgttgagcg ttccctcgcg gggttcgccg gccttgtttt cgatttcggc
      841 cgacacggtc agccgggctt ctttcagcgt tttaagatcg agacgcgttt ggacgaacgg
      901 agaatgaaca accaggtcgc cggtcaggcg gaggcggacc gggcgccaaa tccccatgtt
      961 ggaatcgggc gctttcgggt tccagtcgac gaatcccatg gtcaaagcgc ccggctcgac
     1021 cggaaggatc atgacggcca gggcgtttcg ggctccggga cggatcccgt cggtgacgtc
     1081 gagccggaac tggcggaacg cgccgtagat ttgagatgtg tcggcgatct ttttcccgtt
     1141 cagccagatg tcggcccggt aattgatccc gtcgaattcg aggacggcgc ggccgcggcc
     1201 ggggacggcc gggacgtcga attccgtccg aaaccaccag gggacggcga acggctcggc
     1261 cggaatcttg gcgaagttgt cggccaagaa gatgtccttg tagacgccgt ttttcacgag
     1321 ggcggacaga acggtcgtcg ggatcatggc cgggcgcgcg tcgggaaaag cggcgcccgg
     1381 aagggagaga tcttccccgc cggcggccag gccggccgag gaccggacgg cccagttctc
     1441 cttcagctcg atgatcggaa cggattcggc ggcggccagg atcggcccga ggatcaaggc
     1501 ggacaggacg aagaaggtcg gggagatttt attcatcatg cttctctcct tccgggggga
     1561 tcatcagaat gacgttgttt tattatatcg ccggcttcct ccgcggacaa ggcacgggcg
     1621 gcgtcctgtg atagcaaagc gaaaatgtcc cctttaactg ccaaggtaaa atgtcccctt
     1681 tcgaagggag ggggacatgg aagagaggat cgtattgagc agccgtgaga tccagcgttc
     1741 tttggtctta cagaaggtcc ttgaacgagg attaaccctt aagacggcgg ccaagctgat
     1801 gaagctcggt tatcgccagg ccaagcgtct cttggtccgt cttcgccggg atgggcccaa
     1861 gggattggcc catcgaaatc gaggccggaa agccgccaat gcctatgatc cgaagatccg
     1921 gcacaaggtc ttggccctct cccatggtcg ctatgcccag ttcaacgaca cccactttgt
     1981 ggaaatgttg gcggaacggg aggggctggc gatcagccgg gagaccgtcc gcggttggcg
     2041 cagggaggca gggatcaagg ccaaacggca gcgaagacct ccccgccacc acaagcgacg
     2101 gccgaggcgg ccgatcatgg gcttgctggc ccaatgggat ggaagccccc acccttggtt
     2161 cggctcgcaa catcctccct gctgcctcct ccatgccgtt gacgacgcga ccaatacggt
     2221 cctgggcatg ctgttccggc ctcaggaaga cgccatcggc tatctccgcc tcctggatat
     2281 gatcctccgg cgccacggca tccccgtcgc tatctatcag gaccgccatg cggcgctctt
     2341 tcgtacggat actcactgga gccatgagga ggaactggcc ggaatccgct tccccaccca
     2401 tgtcggccgg gtcctggaag aagtcggcat cgaagccctg gcggccttat cgccccaagc
     2461 taaaggccga atcgaacgtc agggaggaac tttccaggac cggctcgtcg cggaactggc
     2521 cttggccggc attaccgaca tcgagatcgc caacacctgg ttggaaacaa ccttcatccc
     2581 ccgcttcaat gcccgcttcg ccatagcccc gcaacagtcc ggctccgctt tccgtcccat
     2641 caaagccgcc gaccggtatc gcctggtcgc tttcgcttac caagcggtcg tggccaacga
     2701 caataccgtc cgcttgggcc gtctgacgat cgatatccct cccggttcgc ctcgacgctc
     2761 gtatgcccat caaaaggtct tggtgcggca gcacctggac ggggcttgga cggtctggca
     2821 ggaagacgtc caactggctc gtcacgcccc caccccactg cgagagcctt ggaggggttg
     2881 gaaaccccgt gaacacggcg atgaccatcg agctcgacat ctcatccaaa tctatctcca
     2941 gacaacccct gctccgctcg aaggggacat tttcgctcgg cagttagggg gacatattga
     3001 ctcggcttaa acaga
//
LOCUS       DAOOXH010000174         2995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_40170_length_2995_cov_5.627551, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000174 DAOOXH010000000
VERSION     DAOOXH010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40170_length_2995_cov_5.627551"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PKV64_11680"
     CDS             <1..497
                     /locus_tag="PKV64_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17776.1"
                     /translation="WQGTVEFSLGRYNIADPLFNTIYRPGGSITGLGLTANLIPRLGF
                     SLDVKVLSKNGSLSHSKEKTSFVLIPVSLGLRASYPVSFLIPFAGLGLDYYFYFENNP
                     IGTVVDHAKGWHAAAGVYFQFGETIPILPFAKIKYTMVKASAGSRTIDLGGWEYGAGL
                     AIAF"
     gene            529..1917
                     /locus_tag="PKV64_11685"
     CDS             529..1917
                     /locus_tag="PKV64_11685"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPN17777.1"
                     /translation="MNASAAVPKVLLLHCPGDKIYIHDLYCSYSSKADYYWPPSDLII
                     LSGVLRETRLEVIDAVADRLSEEACLERIRRFAPDVVIFTSGTATWKNDFAFTARVKE
                     ETGARLVATGSIFLFEAERFLAAAPHVDAAILDTGTPEIVDYVCGRKRDYSALAVRRA
                     DGLSVPAPARPAPVFTLPVPRHDLFNLRANRSPLARRRPFALVIASIGCPFGCVFCTA
                     GAVAYRSRNLDGVLDELEALRDLGVREIMFNDPTFTVSKKRILELCAGMENRGLRFSW
                     VCNAHITTLDEELAAAMKKAGCHTVMIGVESGQEEILAATDKKITREKAIRAFAVCKK
                     FRIRTLAYFIVGLPGETPDSVGRTIRFAKELDPDFASFTVLTPDVGSPLRQESIAKGL
                     IDPDVLLFDSTCPPVFSSGSLSLEELWRLRQKAVRSFYLRPAYLLKQLRAVRSVRDFL
                     YLLDQARTMFFR"
     gene            1978..2943
                     /locus_tag="PKV64_11690"
     CDS             1978..2943
                     /locus_tag="PKV64_11690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPN17778.1"
                     /translation="MSFSSKSSGTAVFLVLLILAAAVRFWGIGWGLPHTYHADENYFA
                     AKSMRFLEGDLNPHFFHVPTLHMYALAGMWKVYYGIEKAAGNVRTIAEFEETFTQFPQ
                     VFYLIGRFLSALMGAGTVLLLYLLGKRMFGPRAGALAALLLTFGLEHARMSHDMLPDV
                     PMVFFLVLSYLFIWNIAEKGRTRDYLWAGAAAGLAMTTKYGGVLLFIPLFAAHVYRVR
                     EIKRPAWEYILSPRLIGAGLIFLVVFILGSPYVVLDFPKFFKDFRWQSRHLTEEGHFG
                     DSTAHSALLFYLRHGFIENVGIVSQFLVLGGFLLGLFRRGRGIGR"
BASE COUNT          511 a          968 c          841 g          675 t
ORIGIN      
        1 cctggcaggg gacggtcgaa ttttccctcg gccggtacaa catcgccgac ccgctcttca
       61 acacgattta ccggccgggc gggtcgatca ccggtctcgg gctgacggcc aacctgatcc
      121 cccgcctagg tttttcgctc gacgtcaagg tattgtcaaa aaacggttcc ctgtcccaca
      181 gcaaggaaaa gacgtcgttt gtcctcatcc cggtgtctct cgggctccgg gcctcctatc
      241 cggtgtcttt cctgattccg ttcgccggcc tcggcctcga ttattacttc tattttgaaa
      301 acaaccccat cgggaccgtc gtcgatcacg ccaagggctg gcatgccgcc gccggtgtct
      361 acttccaatt cggagagacg atccccatcc tccctttcgc taagatcaag tacacgatgg
      421 tcaaggcctc ggccggaagc cggaccatcg acctgggcgg atgggaatac ggcgccgggc
      481 tggcaatcgc cttttagcca gaggaactgg agagaccgaa gaatgatcat gaatgcttcc
      541 gccgcggttc ccaaggtcct ccttctccat tgcccgggcg ataagatcta catccatgac
      601 ttgtactgca gttactcgtc caaggcggat tattattggc cgcctagcga tctcatcatc
      661 ctttccggcg tcctgcggga aacccggctc gaggtgattg acgcggtggc cgaccgcctc
      721 tccgaagagg cttgtctcga acgcatccgg cgttttgctc cggacgtggt tatcttcacg
      781 agcgggacgg cgacgtggaa aaacgatttc gccttcaccg cccgggtcaa ggaggaaacc
      841 ggggcccgcc tcgtcgccac gggttccatc ttcctcttcg aggcggaacg gttcctggcc
      901 gccgctcccc acgtcgacgc agcgatcttg gatacgggca ccccggaaat cgtcgactac
      961 gtttgcggga ggaagcggga ttactccgcc ctggccgtcc ggagggcgga cggtctgtcc
     1021 gtcccggccc cggcgcgtcc ggctcccgtt ttcactctcc ccgttccccg ccacgatctt
     1081 ttcaacctcc gggccaaccg cagtccgctg gcccggcgcc ggccgttcgc tctcgtcatc
     1141 gcctcgatcg gctgcccgtt cggctgcgtg ttttgcacgg ccggagccgt cgcctaccgg
     1201 tcgcggaacc ttgacggagt cctcgacgag ttggaggccc tccgggacct cggcgtccgg
     1261 gaaatcatgt tcaacgatcc gaccttcacc gtctcgaaga agcggatctt ggaattgtgc
     1321 gccggaatgg aaaaccgcgg actccgcttt tcctgggtct gcaacgccca tatcaccacc
     1381 ctggacgaag agctggccgc ggccatgaag aaggccggct gccataccgt catgatcggc
     1441 gtggaaagcg gccaggagga aatcctggcg gccacggaca agaagatcac ccgggaaaag
     1501 gcgatccggg catttgccgt atgtaaaaaa ttccgcatcc ggacgctcgc ctatttcatc
     1561 gtcggcctgc ctggcgagac gcccgattca gtcggccgga cgatccgctt cgccaaggag
     1621 ctggatccgg acttcgcctc gttcaccgtc ttgactccgg acgtcggctc ccccttgcgg
     1681 caggaaagca tcgccaaggg gttgatcgat ccggacgtcc tgcttttcga cagcacctgt
     1741 ccgccggtct tcagctcggg ctccctgtct ctggaggaac tctggcggct gcggcaaaag
     1801 gccgtccggt ccttttacct ccggccggcc taccttctca agcagctgag agcagtccgg
     1861 agcgtccggg attttttgta tctcctcgat caggcccgga ccatgttttt ccgctgaatc
     1921 ccgggccggc ccggcgattg acgtttccgg aactcttccg ctatattacg attcatcatg
     1981 tccttctcct cgaaatcgtc cgggacggcg gtcttccttg ttctcctcat tctggccgcc
     2041 gcggtccggt tttggggcat cggctggggc cttccccaca cgtaccacgc ggacgaaaac
     2101 tacttcgcag cgaagtcgat gcgcttcttg gagggggatc tcaatcccca tttctttcat
     2161 gtccccacgc ttcacatgta cgccctggcc gggatgtgga aggtttatta cggaatcgaa
     2221 aaagccgccg gaaatgtccg gaccatcgcc gaattcgagg aaaccttcac ccaattcccc
     2281 caggttttct acttgatcgg gcgcttcctc tcggccctga tgggcgccgg gacggttctt
     2341 cttctctatc tgctcgggaa aaggatgttc ggaccgcggg ccggagcctt ggccgccctt
     2401 cttctcacct tcggcctgga gcacgcccgc atgtcgcacg acatgctgcc cgacgtcccc
     2461 atggtcttct ttctcgtttt gtcctatctc ttcatctgga atatcgctga aaaagggcgg
     2521 acgcgggatt atctttgggc cggagcggcg gccggcctgg cgatgaccac caaatacggg
     2581 ggggtgctcc tgttcattcc cttgttcgcc gctcacgtgt accgtgtccg cgaaatcaag
     2641 cggcccgcct gggaatacat cctcagcccg cgcttgatcg gcgccggtct tatttttctg
     2701 gttgtgttca tcctcggcag cccttatgtc gttctcgatt tcccgaagtt tttcaaggat
     2761 tttcggtggc agagccgcca tctgaccgag gaggggcatt tcggggattc caccgcgcat
     2821 tccgcccttc ttttctacct gcgccatgga ttcatcgaga atgtggggat cgtctcccaa
     2881 ttcctcgtcc tcggcggatt cctcctcggc ttgttccgcc gaggacgagg aatagggaga
     2941 tgatccccac attctcgatg aatccatggc gcaggtagaa aagaagggcg gaatg
//
LOCUS       DAOOXH010000175         2952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_41036_length_2952_cov_6.562651, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000175 DAOOXH010000000
VERSION     DAOOXH010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41036_length_2952_cov_6.562651"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKV64_11695"
     CDS             complement(<1..222)
                     /locus_tag="PKV64_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17779.1"
                     /translation="MKNLGFAGVACLLSLFVNCSCSTPPVPPEAARASVLEQDLWRAG
                     GSLLIPRDYEAFRSGLKDADGRIEAEKARF"
     gene            complement(256..843)
                     /locus_tag="PKV64_11700"
     CDS             complement(256..843)
                     /locus_tag="PKV64_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17780.1"
                     /translation="MKNLTKSVVLVLAIAVVLTGCKQPTQLINDVKAAIETVTTAGAD
                     KYAPEELRAVNDDLTKALDEIAAQDKKFFKKFGPAKEMLTALQTKVADLQAALPAKIE
                     AAKNLAIQLQGEAQAAITETTELLKKAPKGKGTAKDLEALKADLEGAETMYTEIQGAL
                     DAGDYIGAQDKANAVKAKVAAIADQINAAIAKVKR"
     gene            complement(1045..2394)
                     /locus_tag="PKV64_11705"
     CDS             complement(1045..2394)
                     /locus_tag="PKV64_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPN17781.1"
                     /translation="MEKVTVEELLRRAAQPAKDAPRLHKYYKGKLGVALKCRVRDFND
                     FAIWYTPGVAAVCKEIAADPQKAYEYTNKWNSVAVISDGTRVLGLGDIGPLGGLPVME
                     GKGILYKYLGGVDAYPICLDEKDPDKIIEIGRALQPSFGGYNLEDIAQPKCFRILDEL
                     RAKCEIPVWHDDQQGTACVTVAGVINALKLVKKDIAAAKFVVLGSGASGINISKMIMA
                     AGANPDNMLSVDSKGILSRDRADRAVLQEKFKEKWDLCMRTNPKAKQGSAADAVVGAD
                     VLIGVSTPGPGTIKAEWIRAMAKDAIVFACANPIPEIWPWEAKEAGARVVATGRSDFP
                     NQVNNSLGFPGIFRGALDVRAKTITDEMCIAAARELAKVAEDKGISEDYIVPNMDEWE
                     VFPREAAAVGMKAIEQGVARFKFTAEELLAMAKETIGKARNEVKLMMNEKFIIDPDQ"
     gene            complement(2520..>2952)
                     /locus_tag="PKV64_11710"
     CDS             complement(2520..>2952)
                     /locus_tag="PKV64_11710"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HPN17782.1"
                     /translation="TIVTARHFFTNLWFHFLRLIGLKKYRPGAVTIQYPEETKPLPPR
                     YRAAHRLMKRPDGKLRCVACLLCATVCPSECIIVEAAEDPDPEIQKTAGRFEIDLTRC
                     CFCGFCVEACPEDAIRMDTGRIDLAARARAELVWTLDDLLR"
BASE COUNT          532 a          889 c          896 g          635 t
ORIGIN      
        1 gaaccgggct ttctcggcct cgatccgtcc gtcggcgtcc ttcaagccgg accggaaggc
       61 ctcataatcc cggggaataa ggagagaacc gccggccctc cagaggtctt gctccaggac
      121 cgaagccctg gccgcctctg gaggtaccgg cggcgtgcta caagaacagt tgacgaacag
      181 actcaggagg caggcaacgc cggcaaaccc aaggtttttc aagcgcgccc gcgattcctc
      241 aagtcccgtc gtcgattacc gcttcacctt cgcgatggcc gcgttgatct gatcggcgat
      301 ggcggcgact ttcgccttga cggcgttggc cttgtcctgg gcgccgatgt agtcaccggc
      361 gtcgagggcg ccctggatct ccgtgtacat cgtttccgcg ccctccaggt cggccttcag
      421 agcttcgagg tccttcgccg tgcccttgcc cttgggggct ttcttcagaa gctcggtggt
      481 ctcggtgatc gcggcctggg cttcgccctg cagctggatc gcgagattct tcgcggcttc
      541 gatcttcgcc ggcagagcgg cctgcaggtc ggcgaccttc gtctgaaggg ccgtcagcat
      601 ttccttcgcc ggaccgaatt tcttgaagaa cttcttgtcc tgagcggcga tttcgtcgag
      661 agccttggtc aggtcgtcat tgacggccct gagctcctcg ggggcgtact tgtcggcgcc
      721 ggccgtagtc acggtctcga tggccgcttt cacgtcgttg atcagctggg taggctgctt
      781 gcagccggtc agaacgacgg cgatcgccaa aacgagaacg acagatttcg tgagattttt
      841 catttcgtcc tccttttctc actcgatcgc gtcactcttc atcggacacg cacatcgaaa
      901 cctaatctcg aagggtatcc tcgtatagac gtcgatctgc actggtattt atcataaaaa
      961 aatatccttg tcaaatgcgc cgaaatacaa aaaaaggggg agggattttc gtccctcccc
     1021 ctcaaataac gagttgaatc cggattattg atccgggtcg atgatgaatt tttcgttcat
     1081 catcagcttg acttcattgc gggctttccc gatcgtctcc ttggccatgg ccaagagctc
     1141 ctcggccgtg aacttgaacc gggccacgcc ctgctcgatg gccttcatcc cgacggccgc
     1201 ggcttcgcgg gggaagactt cccattcgtc catgttcggg acgatgtaat cctcgctgat
     1261 ccccttgtcc tcggcgacct tggccagctc gcgggcggcg gcgatgcaca tctcgtcggt
     1321 gatcgttttg gcccggacgt cgagcgcgcc gcggaagatg cccgggaacc cgagcgaatt
     1381 gttgacctgg ttggggaaat ccgaccggcc ggtggccacg acgcgggcgc cggcttcctt
     1441 ggcttcccag ggccagattt cggggatggg attggcgcag gcgaagacaa tcgcatcctt
     1501 ggccatggcc ctgatccact cggccttgat ggttccgggt cccggagtgg aaacgccgat
     1561 caggacgtcc gcgcccacga cggcgtcggc cgcggatccc tgtttcgcct tgggattcgt
     1621 ccgcatacag aggtcccatt tctccttgaa tttttcctgg agaacggccc ggtcggcccg
     1681 gtcgcgggaa agaatgccct tggaatcgac cgagagcatg ttgtccggat tggccccggc
     1741 ggccatgatc atcttggaaa tattgatccc cgaggcgccc gagccgagaa cgacgaactt
     1801 ggccgcggcg atgtctttct tgacgagctt gagggcgttg atcacgccgg cgacggtgac
     1861 gcaggccgtg ccctgctggt cgtcgtgcca gaccgggatt tcgcacttgg cccgcagttc
     1921 gtcgaggatc cggaagcact tgggctgggc gatgtcctcg agattgtaac cgccgaagga
     1981 gggctggagg gcccggccga tttcgatgat cttatccgga tccttctcat ccaggcagat
     2041 gggataagca tcgacgccgc cgaggtactt gtacaggatg cctttgccct ccatgaccgg
     2101 aaggccgccc aaggggccga tgtcgcccag gcccaggacg cgcgttccgt cgctgatgac
     2161 ggccacgctg ttccatttgt tggtgtactc gtaggccttc tgcggatcgg cggcgatttc
     2221 cttgcagacc gcggccacgc ccggcgtgta ccagatcgcg aaatcattga agtcccgaac
     2281 ccggcacttc aaggcgacgc ccagctttcc tttgtaatac ttgtgaaggc gcggagcgtc
     2341 tttggcgggc tgcgcggctc tcctgagaag ttcctctacc gtaacctttt ccatgcaaga
     2401 cctcctttga aataaagagt ttccgattat acctgattga aaaaagagaa acaatactaa
     2461 acggcgggaa tttcatgaaa agtttcgttt taaaacggag aagcggacgg ccgtccgcct
     2521 caccgcagca gatcgtccaa ggtccagacg agctcggccc gcgcgcgggc ggccaagtcg
     2581 atccggccgg tgtccatccg gatggcgtcc tcgggacacg cctcgacgca gaacccgcag
     2641 aagcagcagc gggtcaggtc gatctcgaac cggcccgcgg tcttctggat ttccgggtcg
     2701 ggatcctcgg ccgcttcgac gatgatgcat tccgaggggc agacggtcgc gcagagaagg
     2761 caggccacgc accgcagctt tccgtcaggc cgcttcatca agcggtgggc cgcccggtat
     2821 cgagggggaa gcggcttcgt ttcctcggga tattggatgg tcaccgcccc cggccggtat
     2881 tttttcaggc cgatcagccg gaggaaatgg aaccatagat tggtgaaaaa atggcgggcg
     2941 gtgacgatcg tc
//
LOCUS       DAOOXH010000176         2904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_41995_length_2904_cov_7.471744, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000176 DAOOXH010000000
VERSION     DAOOXH010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41995_length_2904_cov_7.471744"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1918)
                     /locus_tag="PKV64_11715"
     CDS             complement(<1..1918)
                     /locus_tag="PKV64_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HPN17783.1"
                     /translation="MRIGTRCAAALLAAFVSLGFGIDIRDTRMMTQPAVGKSGLAFHY
                     ANDLWAADLDGKNVRRLTSDLGIESNPAFSPDGGLIAFSAQYDGNTDVYIVPAAGGVP
                     RRLTWHPGADIVQGFTPDGKAVLFTSARQVFTGRYQQLFTVPVEGGFPEKLRIPNAFR
                     AVYSPDGKKMATNPLSDSFLQWKHYRGGTNSTIWIINLDDLSYEKIPQPEGRCNDPGP
                     MWIGDMIYFRSDRDGEFNIYSYDPRTREIKALTRHEDFPVLSASAGDGRIIYEQAGFL
                     HLLDPVSGASTRLQIGIATDAAELRERTAKGAKWIRNGELSPSGARAVFEFRGEIVTV
                     PAEKGDPRNLTRTPGAHERSPVWSPDGKSIAYFSDASGEYALEVRSQDGKGTLRSYAL
                     AGSGFYETPVWSPDGRRIAFHDNARAIYVLDLENGNIKKIGADYQLGGRKSPSAWSPD
                     GKWLAYTLGTASYFQRVFVYSVAQDKSFPVSDGLSEAYRPVFDASGKFLYFFASTDAG
                     PINQWFDLSKFDARLTSSIYVVSLAKDTANPLARESDEETGLPAKSAPGVAAKEAAAP
                     AAPAGPVPAAEVKIDFDGIERRIVAVPVPPGSYDILKAGEAGQIYYLEGPPDIGSPAA
                     ARGGLLHRYDFKSRK"
     gene            complement(1936..>2904)
                     /locus_tag="PKV64_11720"
     CDS             complement(1936..>2904)
                     /locus_tag="PKV64_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011760533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPN17784.1"
                     /translation="RAKDSGTILFTREDYREFPDLWTTDAAFGPARKISEANPQISEF
                     AWGEAELVEWTSDDGRPLQGVLIKPGGYEPGRRYPVLVYYYEISSDRLYDFNEIVVNH
                     RPCFPVYAGHGYAVFLPDIRFTVGRPGMSAVHCLVPGVRKLIDMGLADPKAVGLHGHS
                     WGGYETAFVVTRTDLFAAACAGAPVGNMTSAYSGIRWESGMARQFQYEREQSRIGASL
                     WDARDLYIENSPVFFADRIKTPLLLMHGDEDGAVPWYQSIELYLALRRLGKDCFFLQY
                     RGEPHHPKTYANKLDYSIKMMEFFDHYLKGAPAADWIKTGVPYNGK"
BASE COUNT          525 a          880 c          986 g          513 t
ORIGIN      
        1 ccttgcggga tttgaaatca taccggtgaa gcaggccgcc gcgcgcggcc gcgggagagc
       61 cgatgtccgg agggccctca agataataaa tctgtccggc ttcgcccgct ttcaggatat
      121 cgtagcttcc gggcgggaca ggcacggcga cgatccgccg ttcgatcccg tcaaaatcga
      181 tcttgacctc ggccgccggg accgggcccg cgggcgcggc cggagcggcc gcctccttcg
      241 ccgcgacacc gggggcggat ttggcgggca agcccgtctc ctcgtcgctc tcccgggcga
      301 ggggattggc cgtgtccttg gccagggaca cgacataaat cgagctcgtc agccgggcgt
      361 cgaacttgga caggtcgaac cattgattga tcggaccggc atcggtcgag gcgaaaaagt
      421 aaaggaactt gccggaggcg tcgaaaaccg gccggtaggc ctcgctcaaa ccgtcggaca
      481 cggggaaaga cttgtcctgg gcgacggaat agacgaagac ccgctgaaaa tacgaggcgg
      541 tgcccagggt ataggccagc catttcccat cgggcgacca ggccgaagga cttttccgcc
      601 ccccgagctg ataatcggcc ccgatttttt tgatgttgcc gttctccagg tcgagaacgt
      661 aaatcgcccg ggcgttgtca tggaaagcga tccgccggcc gtcgggcgac cagaccggcg
      721 tttcgtagaa gccggagccg gcgagggcat agctccggag cgtccctttt ccgtcttggg
      781 aacggacctc gagggcgtat tccccggagg catccgagaa ataggcgatc gactttccgt
      841 cgggagacca gacgggagac cgttcatgag cccccggcgt ccgggtgagg ttgcggggat
      901 cccctttctc ggcgggaacg gtcacgatct cgccccggaa ctcgaagacg gcccgggccc
      961 cggagggcga gagctcgccg ttgcggatcc acttggcccc cttggccgtc cgctccctga
     1021 gctcggccgc gtcggtcgca atcccgattt gaaggcgggt cgaggccccg gaaacggggt
     1081 caaggagatg aagaaacccg gcctgctcgt agatgatccg gccgtctccg gccgaggcgc
     1141 tgagaacggg gaaatcttca tggcgggtca aggccttgat ttcccgggtc cgggggtcgt
     1201 agctgtagat gttgaactcc ccgtcccggt cggaccgaaa gtagatcatg tccccgatcc
     1261 acatcgggcc gggatcgttg cagcggccct cgggctgggg aattttttcg taagacaagt
     1321 cgtcgaggtt gatgatccag atggtcgaat tcgtcccccc gcggtaatgc ttccactgga
     1381 ggaaggagtc cgacaggggg ttggtcgcca tctttttccc gtcgggcgag tagaccgccc
     1441 ggaaggcgtt cggaatccgg agcttttccg ggaatccgcc ttcgacggga acggtgaaca
     1501 actgctggta ccggccggtg aaaacctgcc gggcggacgt gaacaggacc gccttcccgt
     1561 ccggagtgaa gccctggacg atgtccgcgc ccgggtgcca ggtcagccgc cggggcacgc
     1621 cgccggcggc cgggacgatg tagacgtcgg tgttgccgtc gtactgggcg ctgaaggcga
     1681 tcaatccgcc gtcgggcgag aacgccggat tggattcgat gcccaggtcg gacgtcagcc
     1741 gccggacgtt ttttccgtcg agatcggccg cccagaggtc attggcgtaa tggaaggcga
     1801 ggccgctttt cccgacggcc ggctgagtca tcatccgcgt gtcgcggatg tcgatcccga
     1861 acccgaggga gacgaaggcg gccagcaggg cggccgcgca gcgcgttccg attctcatga
     1921 tcctcctcct tgatgttatt ttccgttgta aggaacgccg gtcttgatcc agtcggccgc
     1981 cggcgctccc ttcagataat gatcgaagaa ctccatcatt ttgatcgaat aatcgagctt
     2041 gttggcgtag gtcttgggat ggtggggttc gccccggtac tggaggaaga agcagtcctt
     2101 ccccaagcgg cggagagcga ggtaaagctc gatggattgg taccagggaa cggccccgtc
     2161 ctcgtcgccg tgcatcagga gcagcggcgt cttgatccgg tcggcgaaaa agaccgggga
     2221 attctcgatg tacagatcgc gcgcatccca gagagacgcc ccgatccggc tctgttcgcg
     2281 ctcgtattgg aattgacggg ccatcccgga ctcccagcgg atgccgctgt aggcgctggt
     2341 catgttgccc accggggcgc cggcgcaggc ggcggcgaag agatcggtcc gggtgacgac
     2401 gaaggccgtt tcatacccgc cccaggaatg gccgtggagg ccgacggcct tcggatcggc
     2461 gaggcccatg tcgatgagct tccggacgcc cggaacgagg caatggacgg ccgacatccc
     2521 gggccggccg acggtgaacc ggatgtccgg caggaagacg gcgtagccgt ggccggcata
     2581 aaccgggaag caaggccggt gattgaccac gatctcgttg aagtcgtaca gccggtcgga
     2641 cgaaatctcg taataataga ccaggacggg atagcggcgt ccgggctcgt agccgccggg
     2701 cttgatcaag acgccctgga gcggccggcc gtcgtcgctc gtccactcca ccagctcggc
     2761 ctccccccag gcgaattcgc tgatctgggg attcgcctcg gagatcttgc gggccggacc
     2821 gaaggcggcg tcggtcgtcc agagatcggg aaactcccgg taatcctcgc gggtgaacag
     2881 gatcgtcccg ctgtctttcg cccg
//
LOCUS       DAOOXH010000177         2883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_42451_length_2883_cov_4.570014, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000177 DAOOXH010000000
VERSION     DAOOXH010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42451_length_2883_cov_4.570014"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="PKV64_11725"
     CDS             <1..939
                     /locus_tag="PKV64_11725"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HPN17785.1"
                     /translation="TEDQGCLEDADPRAAGARARERGLSQLGTLGSGNHFIEIQIVDE
                     IFDEASAESMGLRKGCLAVQVHSGSRGFGHQICTDYVRSLQSAANKYRILLPDRELAC
                     APISSEEGQAYLKAMRCAANYAYANRQVLAQGVRDAFEQVLAGKGGGRRLRTVYDVAH
                     NIGKIETHAVQGKERAACVHRKGATRSFGPGTKGLPADYRKIGQPVLIPGSMGTRSWV
                     LRGAATSMNKSFGSCAHGAGRLLSRAQAKRQIWGEDLVRELTGRGIHVKAGSLSGLAE
                     EAPEAYKDVEAVIECVAGAGLAEKVAVLRPVAVIKG"
     gene            complement(992..1903)
                     /gene="speE"
                     /locus_tag="PKV64_11730"
     CDS             complement(992..1903)
                     /gene="speE"
                     /locus_tag="PKV64_11730"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HPN17786.1"
                     /translation="MNKQGFYFIEPFTEDFYRFFKLEGVHYEGRTKYQQVHCFKSRLF
                     GKVLFLDNKIQSAQVDEFVYHEALVHPAMITHPSPRRVLVLGGGEGAVLREILKHSPV
                     KRAVMVDIDKELVGLCRKILPEWSRGAFTDPKVKLVFQDARRFVERSKTPYDIILSDL
                     TEPLDGGPSLRLFTREFFERISRTLDGDGLFVLHAGSTDPHYNHFFSSCVKTLTDVFP
                     VVRPYWTSMFSFSLPWGFIIASKNSDPFALDEKEIRRRLAARGVKRLGHYSPQIHRAA
                     FALPPYLKKAIRAGRLITDAEPFVWKA"
     gene            complement(2151..>2883)
                     /locus_tag="PKV64_11735"
     CDS             complement(2151..>2883)
                     /locus_tag="PKV64_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17787.1"
                     /translation="GKSIPQGAGVINLKYDPDKILEQIKKENPEIKEEVYEGVKIFTV
                     PEKEGAPMFGAFLDASNAALGTEAGVKAVIDVMKGKAESVVKNAEIMKLVKTGNTKAL
                     VWNITTFTPEQINKMTETTPMLASLKSLQAATMYIDDKNKGLQIEIKALSSDAAKNKE
                     IADMLTGFKALGAMGSSEKPEIGEFLNKIEISSSPDNVKLFVDMPEALLEKLGQDMEK
                     QVKSKLEGLKPAEEPPAEDVVKEID"
BASE COUNT          526 a          824 c          939 g          594 t
ORIGIN      
        1 accgaggatc aaggctgttt ggaggacgcc gatccccgcg cggcgggcgc ccgcgcccgg
       61 gaacgcggcc tgtcgcaatt gggcaccctg ggatctggaa atcacttcat cgaaatccag
      121 atcgtcgatg agatcttcga cgaggcatcg gcggaatcca tgggactgag aaaaggatgc
      181 ctggcggtcc aggtccattc cggatcgcgg ggtttcggac accagatctg cacggactac
      241 gtccgatcgc tgcagtccgc ggcgaataaa taccgcattc tcctccccga ccgggaattg
      301 gcctgcgccc cgatctcctc cgaggagggg caagcgtatt tgaaggccat gcgatgcgcc
      361 gcaaattacg cttacgccaa ccggcaggtc ctggcccaag gcgtccggga cgcttttgag
      421 caggtcctgg ccggaaaagg cggcggtcgg cgtttgcgga cggtctacga cgtcgctcat
      481 aacatcggta agatcgagac gcacgcggtc caggggaagg agcgcgcggc ctgtgtccat
      541 cgcaaggggg cgacccgcag cttcggaccc gggacaaagg gcctgccggc cgattatcgg
      601 aagatcgggc agcccgtgtt aatccccggc agcatgggaa cccgttcctg ggttttgcgg
      661 ggcgccgcga cgagcatgaa caaatccttc ggctcctgcg cccacggcgc cggaaggttg
      721 ttgagccggg ctcaggccaa gagacagatc tggggggagg acttggtccg cgaattgacc
      781 ggacggggca tccacgtcaa ggcggggtct ttgtccggtc tggccgagga ggcgccggaa
      841 gcgtacaagg atgtcgaggc cgtcatcgaa tgcgtcgccg gcgcgggttt ggctgaaaaa
      901 gtcgcggtct tgagacccgt ggcggtcatc aaaggctgac ggtcggcggc tccccgagcg
      961 ggcccttggg acgcggccgg ccgcggcgcc cctaggcctt ccagacgaac ggttccgcgt
     1021 ccgtgatcag ccgtcccgcc cggatcgctt ttttcagata agggggaagg gcgaaagcgg
     1081 cccgatgaat ttgcgggctg tagtggccga gacgcttcac gcccctggcc gccaggcggc
     1141 gtcggatctc cttctcgtcc agggcgaagg gatcgctgtt tttcgaggcg atgatgaatc
     1201 cccagggaag gctgaacgag aacatggacg tccagtaagg ccggacgacc ggaaagacgt
     1261 ccgtcagggt tttgacgcat gagctgaaaa aatggttgta gtggggatcg gtgcttccgg
     1321 cgtgcaggac gaagagaccg tcgccgtcca gagtcctgga aatcctctcg aaaaactccc
     1381 gggtgaaaag gcggagcgac ggcccgccgt cgagcggctc ggtcagatcc gagaggatga
     1441 tgtcataagg cgttttcgac cgctcgacga accggcgcgc gtcctggaag acgagcttga
     1501 ccttgggatc ggtgaaagcc ccccgcgacc attccggcag gattttccgg caaagcccga
     1561 ccagctcctt gtcgatgtcg accatgaccg cccgcttgac ggggctgtgc ttgaggattt
     1621 cccggagaac ggcgccttcg ccgccgccga gaacgaggac ccggcggggc gagggatggg
     1681 tgatcatcgc cgggtggacg agggcttcgt gatagacgaa ttcgtccact tgagccgatt
     1741 ggatcttgtt gtcgaggaaa aggaccttgc cgaacagacg gctcttgaag cagtgaactt
     1801 gctggtattt ggttcggccc tcgtaatgca ctccctccag cttgaagaac cggtagaagt
     1861 cctcggtgaa gggttcgatg aaataaaagc cttgcttgtt catgatcggt ggatgggaac
     1921 agtctattat agtccccggc cggcgaagcg gcaaccttta taatcgtttg aatcatgatt
     1981 gcggccgccg gccctggatg acggaaagcc gccggccgct tcatcaagac tccgccctcc
     2041 cggcggaaca gcgatcgcgc cgggatccgc gccttatccc gaagaggccg gcggcttgaa
     2101 aagccggttt gatcgatgac ggccgggggc cgtcagtcga cgaccgcgtc ctaatcgatt
     2161 tccttgacca cgtcctcggc cggaggctcc tccgccggct tcaggccttc aagcttgctc
     2221 ttgacctgtt tttccatgtc ctggccgagc ttttcgagga gggcctcggg catgtcgacg
     2281 aagagcttga cgttgtcggg ggaggagctg atttcaatct tgttgagaaa ttcgccgatt
     2341 tcgggctttt ccgacgagcc catcgctccg agggccttga agcccgtcag catgtcggcg
     2401 atttccttgt tcttggctgc gtcggacgag agggccttga tctcgatctg aaggcccttg
     2461 ttcttgtcgt cgatatacat ggtcgccgcc tggagggact tgaggctggc gagcatgggc
     2521 gtggtttcgg tcatcttgtt gatctgttcg ggcgtgaacg tggtgatgtt ccagacaagg
     2581 gctttcgtgt tgcccgtctt gacgagcttc atgatctcgg cgtttttcac gaccgattcg
     2641 gctttaccct tcatcacgtc gatgaccgcc ttgactccgg cttcggtccc gagagcggcg
     2701 ttggaggcgt ccaggaacgc gccgaacatg ggggctccct ctttttccgg aacggtgaag
     2761 atcttcacgc cttcatagac ctcttccttg atctcagggt tttccttctt gatctgttcc
     2821 aggattttgt ccggatcata cttcaggttg atgacgccgg ccccttgggg gatggatttc
     2881 ccc
//
LOCUS       DAOOXH010000178         2875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_42619_length_2875_cov_7.049291, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000178 DAOOXH010000000
VERSION     DAOOXH010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42619_length_2875_cov_7.049291"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PKV64_11740"
     CDS             complement(<1..187)
                     /locus_tag="PKV64_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17788.1"
                     /translation="MSRFLACLGKTLKENFREWKILSLALVFGPFFIFVMYAYFGATS
                     PSYGLLVIDRDKPVAVDG"
     gene            complement(192..1145)
                     /locus_tag="PKV64_11745"
     CDS             complement(192..1145)
                     /locus_tag="PKV64_11745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPN17789.1"
                     /translation="MSESISPTALRLSGLMKRYGDLTAVDGLSLEIRRGEIFGCLGPN
                     GAGKTTTIKMMSGLIRPDGGSVEINGRPVQASSFKGLRTVGLSPQSIVIWETLTCLEQ
                     LVFSGRAYGLDRESARKRSLELLDAFGLSDKKDKLGKTLSGGMQRRLNIALALVHEPD
                     LLFLDEPQAGLDPQSRILVRDYIKSLRGKVTVVLTTHDMEEAEKLSDRVCILDHGRLL
                     VLDTVAAIKDRLGEGDLIEVETREDVRTELLPHLPFRIGEGEARTTDHVLTLISAAAG
                     GVLPGLLQTIRSRGLHLENLRIRPKTLEDVFIKLTGRGLRE"
     assembly_gap    1303..1402
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1467..1928)
                     /locus_tag="PKV64_11750"
     CDS             complement(1467..1928)
                     /locus_tag="PKV64_11750"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPN17790.1"
                     /translation="MKKNFGTAGAGMLAAAFLFCAPRGADKFIKVYFPDGRSVTAELA
                     VTEAERARGLMFRERIAPDQGMLFLMPGEDYHAFWMKNTLIPLDILWLDEDRRIVHIE
                     RDVPPCSGDPCPSYAPSRPGSFVLELGAGGADRYGLKLFDRLDFVLPNPPR"
     gene            complement(1925..2572)
                     /locus_tag="PKV64_11755"
     CDS             complement(1925..2572)
                     /locus_tag="PKV64_11755"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006918690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPN17791.1"
                     /translation="MIRDLDAHREEMVRTQIESRGVVDPSLLRAMRRIPRHFFVHENL
                     RGVAYEDEPLPIGGRQTISQPFIVAFMTEALALKPSDKALEVGTGSGYQTAVLADLAA
                     EVLTVEIIPELAASARAVLSRLGYANIRFRTGDGAEGWPEEAPFDAVIVTAASAQVPD
                     ALTGQLAEGGRMIIPVGRDRQTLRMIRKKGGRLIETALLGVRFVPLITGTCGEAS"
     gene            complement(2599..>2875)
                     /locus_tag="PKV64_11760"
     CDS             complement(2599..>2875)
                     /locus_tag="PKV64_11760"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF020742.2"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPN17792.1"
                     /translation="IQAVLFKEAKAFLEANTRPAATYDEFKSVLETKRGFVVSPWCGD
                     SACEEKVKNETMATIRVIVPGTAAGESSVCIACGRPSRTVAHFARAY"
BASE COUNT          522 a          891 c          835 g          527 t
ORIGIN      
        1 cgccgtcgac ggcgaccggc ttgtcccggt cgataacaag caagccgtag ctcgggctcg
       61 tcgccccgaa ataggcgtac atgacaaaga tgaaaaacgg cccgaagacg agggccaggg
      121 acaggatttt ccattcgcgg aaattttcct tgagcgtctt tcccaggcag gcgaggaatc
      181 tcgacatagc tctactccct caatccccgc cccgtcagct tgatgaacac gtcttccagg
      241 gttttcggcc gaatcctcag gttttcgagg tgaagccccc ggcttcggat ggtctggagc
      301 aatcccggaa gaactcctcc ggccgcggcc gaaatgagcg tcaggacgtg atccgtcgtc
      361 ctcgcctccc cttccccgat ccgaaagggg agatgcggca agagctcggt ccggacgtcc
      421 tcgcgggtct ccacttcgat caagtccccc tcccccagcc ggtccttgat ggccgcgacc
      481 gtgtccagga cgagcaaccg gccgtgatcg aggatgcaca cccggtcgga tagcttttcg
      541 gcctcttcca tgtcgtgcgt cgtcaggacc acggtgacct tgccccgcag cgatttgatg
      601 tagtcgcgca cgagaatccg gctttgggga tcaagcccgg cctggggctc gtccagaaaa
      661 aggagatccg gctcgtggac aagggctagg gcgatattga ggcgccgctg catcccgccg
      721 gaaagcgtct ttccaagctt gtctttttta tcggacaagc cgaaggcgtc cagaagctcg
      781 agcgatcgtt tccgggccga ttcgcggtcc agcccgtagg cccggccgga aaagacaagc
      841 tgttcgagac aggtcagcgt ctcccagatg acaatggact gcggggaaag cccgaccgtt
      901 cgcaatcctt taaacgagga agcttggacg gggcggccgt tgatttcgac cgatcctcca
      961 tcgggccgga tgaggccgga catcatcttg atcgtggtgg tttttcccgc gccgttgggg
     1021 ccgagacatc cgaaaatctc gccgcggcgg atctctaggg acaaaccgtc gaccgcggtc
     1081 aggtcgccgt acctcttcat caacccggac agtctcaggg ccgtcgggga aatactctcg
     1141 ctcatccttc gtccgccttt ccgggacatt gtactccgaa tcagtacctg tttatccatt
     1201 tcgccttttc cggccttccc aatcggtacc tgtttatcca tttccgcatt ttttcccctg
     1261 atacggggaa atgctgaaat ggataaccag gttcggattt gannnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnaggttcgg atttgaggaa atgcggaaat ggataaacag
     1441 gttcggattg gatcggttcc gaatggtcag cgaggtggat taggaaggac gaaatccagc
     1501 cggtcgaaaa gcttcaggcc gtaccgatcg gccccgcccg ccccaagctc caggacgaac
     1561 gatcccggcc gcgacggcgc gtaggacggg caaggatcgc cggagcacgg cggcacatcc
     1621 ctttcgatat ggacgatccg gcggtcttcg tcgagccaga ggatgtccaa gggaatcagg
     1681 gtgtttttca tccagaaggc gtggtaatcc tctcccggca tgagaaacaa catcccctga
     1741 tcgggggcga tcctttcccg gaacataagg ccccgggccc gctcggcctc ggtgaccgcc
     1801 agctcggccg tcaccgaccg gccgtccggg aaatacacct tgatgaattt gtcggctccc
     1861 cgcggggcgc agaacagaaa cgccgcggcg agcatccccg ctccagccgt tccgaagttt
     1921 tttttcacga cgcctccccg catgtcccgg tgatcagcgg gacaaatctc acgccaagca
     1981 gagccgtctc gatcagccgg ccgccctttt tccggatcat ccgcagcgtc tgccggtccc
     2041 gccccaccgg gatgatcatc cgccccccct cggccagctg gccggtcagg gcgtccggca
     2101 cttgggcgga agcggcggtc acgatgacgg cgtcgaacgg cgcttcctcg ggccagccct
     2161 ccgccccgtc gccggtccga aaccggatgt tcgcgtagcc gaggcgggag aggaccgcgc
     2221 gggccgaggc ggccagctca ggaatgattt cgacggtcag cacctcggcc gcgagatcgg
     2281 ccaggaccgc cgtctgatag cccgatcccg tgccgacttc cagcgccttg tccgagggct
     2341 tcaaggccag agcctcggtc atgaaggcga cgatgaacgg ctgggagatc gtctgccggc
     2401 cgccgatcgg cagcggctcg tcctcgtagg cgacgccccg gaggttctcg tgaacgaaga
     2461 aatggcgggg aattctccgc atggcccgca gcagcgaggg atcgacgacg cccctggatt
     2521 cgatctgcgt ccgaaccatt tcttcccgat gcgcgtccag atctctgatc atgcctcctc
     2581 aagcggcggc cggccggctc agtacgcccg ggcgaaatgg gcgacggtcc gggaaggccg
     2641 gccgcaagcg atgcatacgc tcgattcccc ggccgcggtt ccgggaacga tcacccggat
     2701 cgtggccatg gtttcgtttt taaccttttc ctcgcaggcc gaatcgccgc accacgggct
     2761 gacgacaaaa ccgcgtttcg tttcgaggac ggacttgaac tcgtcgtacg tcgcggccgg
     2821 ccgcgtgttg gcctcaagaa aggctttcgc ttccttgaac aacaccgcct ggatc
//
LOCUS       DAOOXH010000179         2818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_43845_length_2818_cov_6.609844, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000179 DAOOXH010000000
VERSION     DAOOXH010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43845_length_2818_cov_6.609844"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1496)
                     /locus_tag="PKV64_11765"
     CDS             complement(9..1496)
                     /locus_tag="PKV64_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPN17793.1"
                     /translation="MPKVQLYVPPGGYFAERWSKGSSIPPLGLLSIGAVLEREGIPVE
                     IVPADILGLSWSDIVRKIRADRPDIVGVTITTENRFQSFRLVRLAKKARPEVLTILGG
                     PHASMAAEDCLDHIPELDLVCRGEGEETMLEVCRRWTPGSGAAVLGDVRGLVLRRDGR
                     ASANPPRPPIADLDTLPSPAFHLVPFEKYNFTLDVPGRGPLRAISVMTSRGCPFACSF
                     CATPINWGRHVRMRSPENVVREIELLKERYGIEAVFFYDDTFNASPRRADAICDLMIA
                     RGLDVRFMCDVRLDIMSRELIEKMKRAGLYYLSYGLEAGSDRVRKDIVGKQIDLADFH
                     RLTGWCLELGIVANVFMIFSHPTETWTEAQETIRLIEKYRGRIETSIAILHVYPGTPL
                     EKTARERGVLPPGFTWTKRYRKGVITLPLAQGDVPLFIDKLRWSQICELVLRWSLSSR
                     KSSPLKKAPRALRKIRSWGDFARLAVMGAKYLQLKFFGFRTKSRA"
     gene            complement(1489..>2818)
                     /locus_tag="PKV64_11770"
     CDS             complement(1489..>2818)
                     /locus_tag="PKV64_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPN17794.1"
                     /translation="HELEILAMDKAALGEKMKTHAALVERLLEEKNALQAEGEERERE
                     LAVHQDKQNADSTGLATAKGEVHVLEAKIKSLEDLCLALARRKEASQIKIDELQAGVR
                     SSLEEAGRIKTAMADSAGKITGFQTRQEELEKSLAEGEIRLSTARKDLETAEGSLSGR
                     RMEHERLKDERMAWEVRKAEIERDMVNLEEACWTELKKNLHEIRSEPLPEREPGIPEP
                     TAAEVEADLENAREDMAKYKTVNLMAEEEYAEQKQRHDFLIAQRDDLRTSISQTKEAI
                     SQINGESRDRFLAALTEINKNFQDLFITLFKGGTAEVRLIDEANPLESGVEVIAQPPG
                     KKLANMGLLSGGEKSLTSLAFLFALFRFKPTPFCILDEIDAALDEPNLVRFLDLMKTI
                     KSETQFIIITHNYKTMEVADYIYGTTMEEPNVTRVFSMKLDKKPEAETDA"
BASE COUNT          471 a          845 c          915 g          587 t
ORIGIN      
        1 agcgtatatt atgcgcgact cttcgtccgg aacccgaaaa acttgagctg gaggtatttc
       61 gcgcccatca ccgcgagacg ggcgaaatcc ccccaggagc ggatctttcg gagcgcccgg
      121 ggagcttttt taagcggaga ggatttccgg gagctgagcg accagcgcag gacgagctcg
      181 cagatctggg accagcggag cttgtcgatg aacaagggaa cgtcgccctg ggccaggggg
      241 agggtgatga cccccttgcg gtaccgtttc gtccacgtga atcccggcgg aagaacgccc
      301 ctctcccggg cggttttttc caggggcgtg cccggataca catggagaat ggcgatcgaa
      361 gtctcgatcc ggccccggta cttttcaatg aggcggatcg tctcttgggc ttccgtccat
      421 gtctcggtcg gatgactgaa gatcatgaaa acgttggcca cgatcccgag ctccaagcac
      481 cagccggtga ggcggtggaa gtcggccagg tcgatctgtt tgccgacgat gtccttccgg
      541 acccggtccg agccggcctc gagcccgtag gacaggtagt aaagcccggc ccgcttcatc
      601 ttctcgatca gctcccggct catgatgtcg agacgaacgt cgcacatgaa gcggacatcg
      661 agtccgcggg cgatcatcaa gtcgcaaatg gcgtcggccc ggcgggggct ggcgttgaac
      721 gtgtcgtcat agaagaaaac ggcctcgatg ccgtagcgct ccttcaggag ctcgatttca
      781 cggacgacgt tttccggact gcgcatccgg acgtggcgtc cccagttgat cggtgtggcg
      841 cagaaggagc aggcgaaggg acagccccgg ctggtcatga cgctgatcgc ccggaggggg
      901 ccccggcccg ggacatcgag ggtgaagttg tatttctcga agggaaccaa gtggaaagcg
      961 ggagagggca gcgtatcgag gtcggcgatc ggcggacgcg gcggattggc cgaagcccgg
     1021 ccgtcgcggc ggagaaccaa gccccggaca tctcctaaaa ccgcggctcc gctccccggc
     1081 gtccagcgcc ggcagacctc gagcatcgtc tcctcgccct cgccccggca gaccaggtcg
     1141 agctcgggga tatgatccaa gcaatcctcg gcggccatcg aggcatgggg cccgcccagg
     1201 atggtgagga cttccggacg ggcttttttg gccaggcgga cgagacggaa gctctggaac
     1261 cggttttcgg tcgtgatcgt caccccgacg atgtcgggcc ggtccgcccg gatcttgcgg
     1321 acgatgtccg accagctcag ccccaagatg tcggccggga cgatttccac cgggatcccc
     1381 tcccgttcca ggacggcccc gatcgagagg aggccgagcg gcgggatgga cgaccctttc
     1441 gaccagcgct cggcgaagta tccgccgggc ggcacgtaga gctggacttt aggcatcggt
     1501 ctccgcctcc ggcttcttgt cgagcttcat ggagaagacc cgcgtgacgt tgggctcctc
     1561 catggtcgtt ccgtagatgt aatcggccac ttccatggtc ttgtagttgt gggtgatgat
     1621 gatgaactgg gtttcggact tgatggtttt catcaggtcc aggaaccgga cgaggttggg
     1681 ctcgtccagg gccgcgtcga tctcatcgag gatgcagaag ggcgtcggct tgaaccgaaa
     1741 cagggcgaac agaaaggcca ggctcgtcag ggatttctct ccgcccgaga ggagccccat
     1801 gttggcgagc ttcttgccgg gcggctgggc gatgacctcg acgccgcttt cgagcggatt
     1861 ggcctcgtcg atcaagcgga cctcagccgt ccctcccttg aacagggtga tgaagaggtc
     1921 ctggaagttt ttattgattt ccgtcagggc ggccaggaaa cggtcccggc tttcgccgtt
     1981 gatctgggag atcgcttcct tcgtctggga gatcgaggtc cggaggtcgt cgcgctgggc
     2041 gatgagaaag tcgtggcgct gcttttgctc ggcgtactcc tcctcggcca tcaggttgac
     2101 ggtcttgtac ttggccatgt cttcccgggc gttttcgaga tccgcttcga cctcggcggc
     2161 ggttggctcc ggaatccccg gctcccgctc cggaagcggt tcggaacgga tttcgtggag
     2221 gttttttttc aactcggtcc agcaggcctc ctcgaggttg accatgtcgc gctcgatctc
     2281 cgccttgcgg acctcccagg ccattcgttc gtccttgagc cgttcatgct ccatccgccg
     2341 gccggacaaa gatccctcgg cggtttcgag atccttccgg gccgtcgaca ggcggatttc
     2401 accctcggcc agggattttt ccagttcctc ttgccgggtt tgaaagccgg tgatttttcc
     2461 ggcggaatca gccatggcgg ttttgatccg tccggcctcc tcgagcgagg agcggacgcc
     2521 cgcctgcagc tcatcgattt taatttggga ggcctctttc cgccgggcca gggccagaca
     2581 aagatcctcg aggcttttga tcttggcttc gaggacgtgg acttcgccct tggccgtggc
     2641 caagccggtc gaatcggcgt tctgcttgtc ctggtgaacg gcgagctcgc gttctctttc
     2701 ctctccctcg gcttggagag cgtttttttc ctccaggagc cgctcgacca gggccgcgtg
     2761 agtcttcatc ttttccccga gggcggcctt gtccatggcc aggatctcga gctcgtgg
//
LOCUS       DAOOXH010000180         2818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_43853_length_2818_cov_4.522620, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000180 DAOOXH010000000
VERSION     DAOOXH010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43853_length_2818_cov_4.522620"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PKV64_11775"
     CDS             complement(<1..865)
                     /locus_tag="PKV64_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17795.1"
                     /translation="MNASRPIIGVKIYENRGGFDRLFAEWKELGINTAFVGEALLADP
                     VFRTGARANGITLYVIFPVFQNPEELQERPDLAAVTDRGESAREEWVQFVCPTRKEYK
                     DRRIEHLRRIVREHDPDVVSLDFIRFFVFWEMVLPGRTLESLPRTCFCPSCLESFRTT
                     TGIVWPDTLAGTPAVARWMLDHHPAEWTDWKCRVIADWARDLAAAARQVKPGIKINVH
                     LVPWRAEDFGGAARAVAGQDPIRLAAAADFLSPMCYHHMVGQTPAWVHSVVEELSSRT
                     GAAVLPSIQVGH"
     gene            1347..2414
                     /locus_tag="PKV64_11780"
     CDS             1347..2414
                     /locus_tag="PKV64_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HPN17796.1"
                     /translation="MKRRKFLAQAWPAALAAAPPVFGALRAGRPARARSEKMSPRTED
                     VKRAMLAMQRDAWEQGTAAQALLEMGETEEVILMAKEAAVRQTPDGRLAVLSCFGGVT
                     DPAANGEAMLFAARKTGDPRLFQAAEKMLSYLLERAPRRADGTLYHVSDKKQVWIDSL
                     YMAPPFLAVAGRPDEAVRQVEGIRRVLWNPEAGLFSQIWDDDRGEFERKDFWGVGNGW
                     AAAGMTRVIRALPETMTGEKKALAGHVREVIDGCLRRQRDDGLFHDVLDRPDTFVETN
                     LAQMLAYSIYRGIAGGWLDPALQKPADRMREAARGKVDAFGLVQGVCGSPEFSSAGTA
                     TEGQAFFLLMETAAADLADSR"
     gene            2441..>2818
                     /locus_tag="PKV64_11785"
     CDS             2441..>2818
                     /locus_tag="PKV64_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17797.1"
                     /translation="MMKTTAGLRTPLILAALFLIAGGGEGFGSAAIPGEDLRAGKNFL
                     PTPVTTEKEDADVLALFSGLRVADVADGMDAAGLQNIGLMDPSIHPLWRDTEHFTHRF
                     AGIAVTARYVPTQSPPAGKREAAD"
BASE COUNT          530 a          927 c          911 g          450 t
ORIGIN      
        1 catggccgac ctggatgctg ggaaggacgg ccgcgccggt ccgggaagac aactcctcga
       61 cgacggaatg gacccaggcc ggcgtttgcc cgaccatgtg gtgatagcac atcggcgaca
      121 aaaaatcagc cgccgccgcc agccggatcg gatcctgccc ggccacggcc cgggccgctc
      181 cgccgaagtc ttccgccctc cagggaacga ggtgaacgtt gatcttgatc cccggcttga
      241 cttggcgggc ggccgcggcc aggtcccgcg cccaatcggc gatcacccgg cacttccagt
      301 ccgtccattc cgccgggtga tgatcgagca tccagcgggc gacggccgga gtccccgcga
      361 gcgtgtccgg ccagacgatc cccgtcgtcg ttcgaaagct ttccaggcac gacgggcaga
      421 agcaggtccg gggcagggat tccaacgtcc ggccgggcag gaccatctcc cagaaaacaa
      481 aaaagcggat gaaatcgagg ctgacgacgt ccgggtcatg ttcccggacg atccggcgga
      541 gatgttcgat ccggcggtcc ttgtattcct tccgggtcgg acagacgaat tggacccatt
      601 cctcgcgggc cgactccccc cggtccgtga ccgcggccag gtccggccgt tcctgaagct
      661 cctcgggatt ctgaaaaacc gggaaaatga cgtacagggt gatgccgttg gcccgggcgc
      721 cggtccggaa gaccggatcg gccagaaggg cttccccgac aaacgcggtg ttgatcccca
      781 gctccttcca ttccgcgaag agacggtcga agccgccccg gttctcgtag atcttcacgc
      841 cgatgatcgg ccgggacgcg ttcatttccc tcttcccggc ggccggcgaa ccggcccgga
      901 gacaaatgaa agatatcaat aaagaaagaa tgagagccgc cggccgtctt attcttttcg
      961 acttcatggt tcctccatcg ggacgatgtc ccgcaaaggg aaatccgccg cgataacgcc
     1021 gccctcccgc cggacaagat tccggaacgc tttgcccctc agccggcgaa acacatggag
     1081 attcttccgc tgcggcgtgc ccgttggaca ccccgtttga aacatcgccg cccgcgggac
     1141 aacgcttctc cttgataaag cgggggtctc ctcaattcga tagaatcgtc tcctcccgac
     1201 gaaaagtcaa ggacggcggc cctccggaga agacgtccgg atgaagaagg cctcccggcc
     1261 tatcgaagat caaggatgat ttcttttgtc aatttgaacg gccccgggaa atggcctata
     1321 ctcgggccgg acggcgtgga ggaatcatga agagacgcaa attcctcgct caggcttggc
     1381 cggccgcgct ggcggccgca ccgccggttt tcggcgcgct ccgcgccgga cgtcccgcgc
     1441 gcgcccgatc cgaaaagatg agcccccgga cggaagacgt gaagcgggcc atgctggcca
     1501 tgcagcgcga cgcctgggaa cagggcacgg ccgcccaagc cctccttgaa atgggcgaga
     1561 cggaagaagt gatcctgatg gccaaggaag ccgccgtccg gcaaaccccg gacggccgcc
     1621 tggccgtcct cagttgtttc gggggagtca ccgatccggc ggccaacgga gaagcgatgc
     1681 tcttcgccgc ccggaagacg ggggatccgc ggcttttcca agcggccgaa aaaatgctct
     1741 cctacctgct cgaacgggcg ccccgccggg cggacgggac gctctaccac gtctccgaca
     1801 agaagcaggt ctggatcgat tcgctgtaca tggcccctcc gtttctcgcc gtcgccggac
     1861 ggccggacga agccgtccgc caggtggagg ggatccgccg ggttctctgg aatcccgagg
     1921 ccggcctctt ttcccagatc tgggacgacg accgggggga attcgagaga aaggatttct
     1981 ggggtgtggg caacggctgg gccgcggccg ggatgacccg ggtcatccgg gcgcttccgg
     2041 aaaccatgac cggagaaaag aaagccctgg ccggacacgt ccgggaggtg atcgacggct
     2101 gcctcaggcg gcagcgggac gacgggctgt tccacgatgt cctcgaccgc ccggacacgt
     2161 tcgtcgaaac gaacctggcc cagatgctcg cctattccat ctaccggggg atcgccggcg
     2221 gctggctcga tccggctctt caaaagccgg ccgaccggat gcgggaagcg gcccgcggaa
     2281 aagtcgacgc gttcggcttg gtccagggcg tctgcggctc ccccgagttc agctccgccg
     2341 gaacagcgac cgaaggccag gccttttttc tcctgatgga gacggccgcg gccgatctgg
     2401 cggattcccg ctgattcaaa ggaagggaga ggagaaaccc atgatgaaaa cgacggccgg
     2461 cttgcggacg ccgttgatcc tggcggcgct tttcttgatc gccggaggcg gggagggatt
     2521 cgggtcggcc gccatccccg gcgaggatct ccgggccggg aaaaatttcc tgccgacgcc
     2581 ggtcacgacg gaaaaggagg acgcggacgt cctggccctc ttttccggcc tgcgggtcgc
     2641 cgacgtggcc gacggaatgg acgccgccgg ccttcagaac atcgggctga tggatccctc
     2701 gatccatcct ctctggcgcg atacggaaca cttcacccac cggttcgccg ggatcgccgt
     2761 caccgcccgc tatgtcccca cccagagccc gccggccggg aagcgggagg ccgcggac
//
LOCUS       DAOOXH010000181         2785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_44609_length_2785_cov_7.790476, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000181 DAOOXH010000000
VERSION     DAOOXH010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44609_length_2785_cov_7.790476"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PKV64_11790"
     CDS             complement(<1..388)
                     /locus_tag="PKV64_11790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HPN17798.1"
                     /translation="MPLMASSAPMLILKTGLGLLPVLIFLLILVFLDSYKLVRPRALL
                     AALAGGAAAAVLGYASNGALLPVFRNDFTFYSRIVSPLLEESLKAAVLFFFFFRSRKI
                     GFLVDAAILGFSVGAGFSLVENTVYLN"
     gene            609..2651
                     /locus_tag="PKV64_11795"
     CDS             609..2651
                     /locus_tag="PKV64_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17799.1"
                     /translation="MTKKQRGLLIAASAAAAVLAVLFFFHPGWLTKNTDGDQMIGTIS
                     RADRYRPGGMTAKDVVLTKADVTKLLQDPAFQNLIKNVEFQRLLGDPRFAAMMKEDQA
                     GASRLLGDPRFTGLFQAAADLNAVSNDGEEEAESGFESLVKNADFIGLLKNPDLPRII
                     ESAAAAGMFESAAFYAMMGDKTLAGLLQRPEMKALAGNGSFARYVLNEPKYAPVIQRI
                     ETAGAWGAFREALAGGRLDESGPSPVPADLARDSAFLEFFNDLDVAALHKDKTFLSLV
                     ESADFARLVRNLNAAGVYSDGGRAFSALINGPGFSGFLKALDSLSDADRADVLRTAGD
                     ANFRELLGNEDFPRVYARPEFPEVVKALAAADLRFEGAEAFEGPITRRMIELVCPDCP
                     EVYALAQKTFFLALSRNAAFVRIVENNALKIVMAEIRGLRDLIQSFPSGLVLVYSDPR
                     QKELFKSAGFQTLLADRSFLLLLADPNFRTLLSDIKSLADLIGFWSVQAGSDSVPPPS
                     LLANPAFAALHNDARYRLLLAGVSLPAALGSPAGFFFLADSSAVSQVKSMVQNGGGRG
                     AALLADDRLARIIVADPARTAIVLAHPGFFALAEHASRILADQGAARFDQFVGVLQNP
                     KTMLLLKNAPFCSLMRDPAFAGLLFSGTIQAVLNNPPLTEMLNDSMSGALFQNTIN"
BASE COUNT          534 a          861 c          846 g          544 t
ORIGIN      
        1 catttaaata aacggtgttt tccacgaggg aaaagccggc gcccacggaa aatcccagga
       61 tggcggcgtc gaccagaaag ccgattttcc gggaacggaa aaagaaaaaa aagaggacgg
      121 ccgccttgag gctttcctcg agaagagggg aaacgattct cgagtagaag gtgaaatcgt
      181 tccggaatac cgggagcaag gcgccgttcg aggcatatcc caggacggcc gcggccgctc
      241 cgccggccaa ggcggccagc agcgccctcg gccggaccag cttgtagctg tccaagaaaa
      301 ccaggatcaa aagaaaaatc agaacgggga gaaggccgag gccggttttc aggataagca
      361 tcggggcgga cgaggccatg agcggcattt ttttcattct atgccacccg cccggccgga
      421 gcaagccggg atcgccggtt ttcttaattg acacgtcctt cgagaaagac gtatattccg
      481 ggtgtcttcg aaaatcaggg agcggagcgt tcacgtcgac gaggggcccg cgagcctcgc
      541 ggggcggggt ctgccgcgtc cggggacaag accgctttcc ggaatctcaa catcagggag
      601 gggatcggat gacgaaaaag cagagaggat tgttgatcgc cgcctcggcc gcggccgccg
      661 ttttagccgt tcttttcttt tttcatcccg gctggttgac aaaaaacacc gatggagacc
      721 aaatgatcgg gacaatctcc cgggccgatc gctaccgccc cggcggaatg acggccaagg
      781 atgtcgtcct gaccaaagcg gacgtcacga aattgctcca ggatcccgct tttcagaacc
      841 tgatcaagaa cgtcgaattc cagcgcctgc tcggcgatcc acgcttcgcc gccatgatga
      901 aggaggatca ggcgggggct tcccgtcttt tgggtgatcc gcggttcacc ggcctcttcc
      961 aggcggccgc ggacctgaac gcggtttcca acgacgggga ggaagaagcg gagtccgggt
     1021 tcgaatcgct ggtcaagaac gcggatttca tcggccttct caagaatccc gatcttcccc
     1081 ggatcatcga atcggcggcc gccgccggga tgtttgaaag cgcggcgttc tacgcgatga
     1141 tgggggacaa aaccctcgcc ggactccttc agcgcccgga gatgaaagcc ctggccggca
     1201 acggaagctt cgcccgatat gtcttgaacg agccgaaata cgctccggtg atccagcgga
     1261 tcgagacggc cggcgcctgg ggagcgttcc gggaagccct ggccggaggc cgtctcgacg
     1321 aatccggccc gagtccggtt ccggccgatc tcgcccggga ctcggccttc ctggagtttt
     1381 ttaatgatct cgacgtcgcc gccttgcata aagacaagac gttcctctcc ctcgtcgaat
     1441 cggcggattt tgcccgcctt gtccggaatt tgaacgcggc cggcgtttat tccgacggcg
     1501 ggcgggcgtt ctcggccctg atcaacgggc cgggattctc cggattttta aaggcgctcg
     1561 attccttgag cgacgccgac cgggcggacg ttctccggac cgccggcgac gcgaacttcc
     1621 gcgagcttct cggaaacgag gattttccac gcgtttatgc ccgtcccgag ttccccgaag
     1681 tggtgaaggc tttggcggcg gcggatctcc ggttcgaagg agcggaagct ttcgaagggc
     1741 cgatcacccg gcggatgatc gaactcgtct gtccggactg tcccgaggtg tacgccttgg
     1801 cccaaaaaac ctttttcctt gccctatccc gcaacgcggc cttcgtccgg atcgtggaaa
     1861 acaacgccct caagatcgtg atggccgaaa tccgggggct gcgcgatctg attcaaagct
     1921 tcccctccgg actcgtcctt gtctatagcg atccccggca gaaggagctt ttcaagagcg
     1981 ccggattcca aacccttctg gcggacagga gcttccttct cttgctggcc gatccgaatt
     2041 tccggacgct cctgtccgac atcaaatctc tggccgatct catcggcttc tggtcggtcc
     2101 aagccggttc ggattccgtc ccgccgccgt cgttgctggc caatccggcc ttcgccgctc
     2161 ttcataacga cgcccggtac cgcctcctgc tggccggcgt ctccctgccc gccgccctcg
     2221 gttccccggc cggcttcttc tttctagcgg attccagcgc ggtttcccag gtgaaatcga
     2281 tggttcagaa cggaggcgga agaggagccg cgctgcttgc cgatgatcgc ctggcccgga
     2341 ttatcgttgc cgacccggcc cggacggcga tcgtcctcgc ccaccccggc ttcttcgccc
     2401 tggcggaaca cgcaagccgg attctggccg atcaaggcgc agcgcgtttc gatcaattcg
     2461 tcggcgtgct ccagaatcca aaaacgatgt tgctgctcaa gaacgcgcct ttctgctcgc
     2521 tgatgcggga tccggcgttc gccgggctgc tgttcagcgg gacgattcag gccgtcctca
     2581 acaatccgcc tctcaccgag atgctgaatg actccatgag cggggctctt ttccagaaca
     2641 cgatcaactg agaataaaca gcccgccgcg aggacgattt tccggacgcc gggacgtccg
     2701 ttcggccggc ggggaggaga ggatgatgac gacgcgccga cggccgggcg tgaccgcttg
     2761 tcttttcgtc ctggcggcgt tcgcc
//
LOCUS       DAOOXH010000182         2782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_44677_length_2782_cov_5.823616, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000182 DAOOXH010000000
VERSION     DAOOXH010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44677_length_2782_cov_5.823616"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PKV64_11800"
     CDS             <1..830
                     /locus_tag="PKV64_11800"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17800.1"
                     /translation="SVKIPKGPLPDPKDRMNEFKLLYDAKLAEYEGRLDESENNYRMV
                     LEMAPDVSWHYISLAILMARQKRFDEAIKVLKTGIDRIPDSVVLMSRLASFYMRVGDF
                     KDAYEMSVATLALDPRHFDALVIAGWSEDMRGNWKASADFFGRALEIEPENKFTRLKY
                     AYALGALGRGEEAAAIDEKLKSEYPKDGRIYKDLGVIYTALRKMDLAEENLAKAVEYS
                     PNPDNYLAYAAILERVGKLPEAVVNLKLYLENTREGNTPRKQQAQKVLAEWEKRIKE"
     gene            859..>2782
                     /locus_tag="PKV64_11805"
     CDS             859..>2782
                     /locus_tag="PKV64_11805"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPN17801.1"
                     /translation="MSIRKKRNGRLGRSKRRIASVLLIPAAAGISLVLSAGAADKVKK
                     EAGLNVLLVTLDTTRADRLGCYGFAKARTPNLDSLAAGGVRFANAYCPAPMTLPSHCS
                     ILTGTYPIYHKVHNNGTYYLNPEILTLTERFKENGYRTAAFVSSFNVDSRFGTDKGFE
                     VYDDTYGRDEMIKTFRSERSAADTSAAFIGWLDGLGGAKFFSWIHYYDPHMPYRPPSP
                     FREQFPGDPYDGEIASMDHAFGAVLAKLKEKNLLDKTLIVVAGDHGEALGEKGEVDHG
                     VLIYDVSMRIPFILFAPRNLPAGKVVDARVRLIDIMPTVLDLVKLPSVKNVQGASLLP
                     FIEGKRTDDLTCYLESYYPLETYGWSELVGVIDKDWKYIRAPRPELYNIKTDKDENRN
                     LYESDPQTAAALRKKLDDLLRENTSNLVLSRRKMSLVEEERLRSLGYVGGDGAVKLPK
                     GPLPDPKDKMGEFRILMGAVQSEWNGDMAAAEKNYREMIRLAPDVAWRYLDLAIFLSK
                     NNRVSEAAAVLEQGLTRLPDAFPLYSRLAHFYMRLGKLQEALDMSRAALRMDPRYFDA
                     LVIAAAVEDVRGNWAESVKYYKTALDVEPENLMVGMKYAYALAAAGSGEEAAAIDEAL
                     KRDNPKDFRIYLDLGVI"
BASE COUNT          585 a          861 c          837 g          499 t
ORIGIN      
        1 cctcggtgaa aatccccaag ggaccgctgc ccgatcccaa ggaccggatg aacgagttca
       61 agcttctcta tgacgcgaag ctggccgaat acgaggggcg cctcgacgaa tcggaaaaca
      121 attaccggat ggttctcgaa atggccccgg atgtctcctg gcattacatc agcctggcca
      181 ttctgatggc ccggcagaag cgcttcgatg aggccatcaa ggtgctcaag accggcatcg
      241 accggatccc cgactccgtc gtcctcatgt cccggctggc cagcttttac atgagggtcg
      301 gcgatttcaa ggacgcttac gagatgagcg tggccacctt ggctctcgat ccccggcatt
      361 tcgacgccct ggtcatcgcc ggctggtcgg aagacatgcg gggcaattgg aaggcctcgg
      421 ccgacttttt cgggagggcc ctggaaatcg agccggaaaa caaattcacc cggctcaagt
      481 acgcttatgc cctgggcgcc ctgggacgcg gggaggaagc cgcggcgatc gacgaaaagc
      541 tgaaatcgga atatcccaag gacggccgga tctacaagga tctgggcgtc atttacacgg
      601 cgctcaggaa gatggacctg gccgaggaaa acctcgccaa ggcggtcgag tacagcccca
      661 atcccgacaa ttacctggcc tacgcggcca tcctggaacg ggtcggcaag cttcccgagg
      721 ccgtcgtcaa tctgaagctt tatttggaaa acacgcggga gggcaacacc ccccggaagc
      781 agcaagccca aaaagtcctg gccgaatggg aaaagcggat caaggaatag gggatccccc
      841 ttccttgagg atgtctcgat gtcaatacgc aagaaaagaa acggccggct cgggcggtcg
      901 aagcgccgga tcgcctccgt cctcttgatt ccggcggccg ccgggatcag cctggtcctg
      961 tccgcggggg ccgcggacaa ggtgaaaaag gaggccgggc tcaacgtcct tctcgtcacc
     1021 ctggacacga cccgggccga ccgtctcggc tgttacggat tcgccaaagc ccggacgccg
     1081 aatctggatt ccctggcggc cggcggcgtc cggttcgcca acgcctactg ccccgccccg
     1141 atgacccttc cctcccactg ctcgatcctg acggggacgt atcccatcta tcataaggtt
     1201 cacaacaacg ggacctatta tctcaacccc gagattctca ccctgaccga gcggttcaag
     1261 gagaacggct accggacggc ggcgttcgtc agctccttca acgtcgattc ccggttcgga
     1321 acggacaagg gattcgaggt ctacgacgat acgtacggcc gggacgaaat gatcaagacc
     1381 ttccgctcgg agcggtcggc cgcggacacg tcggccgcct tcatcggctg gctggacggc
     1441 ctgggcggag cgaaattctt ttcctggatc cattattacg atcctcacat gccctatcgt
     1501 ccgccctctc cgttccggga gcagttcccc ggagatccct acgacggcga aatcgcctcg
     1561 atggatcatg ccttcggcgc ggttttggcc aagctcaagg aaaagaacct cctggacaag
     1621 acgctgatcg tggtcgccgg cgatcacggc gaagccctgg gggaaaaagg cgaagtggac
     1681 cacggcgtgc tgatttacga tgtgtcgatg cgcattccct tcattttatt cgcgccgcga
     1741 aaccttccgg cggggaaggt cgtcgacgcc cgcgtccggc tgatcgacat catgcccacg
     1801 gtcctggacc tggtgaagct tccttccgtc aagaatgtcc agggcgcgag cctcctcccc
     1861 ttcatcgagg ggaagagaac cgatgatctc acctgttacc tggaaagcta ttaccccctg
     1921 gagacgtacg gctggtcgga gcttgtcggg gtcatcgaca aggactggaa atacatccgg
     1981 gccccccggc cggagctgta caacatcaaa acggacaagg acgaaaaccg gaatctttac
     2041 gagtccgacc cgcaaacggc cgcggccctc cggaagaagc tcgacgatct tctccgggag
     2101 aacacctcga acctggtcct gagccggcgg aaaatgagcc tggtggagga ggaacgcctg
     2161 cggtcgctcg gctacgtcgg cggcgacggc gcggtgaagc tccccaaggg tccccttccc
     2221 gatccgaaag acaagatggg ggagttccgc atcctgatgg gcgccgttca gagtgaatgg
     2281 aacggggaca tggccgccgc cgagaaaaac taccgggaaa tgatccgcct ggcccccgat
     2341 gtcgcttggc gctacctcga cctggcgatt ttcctttcga aaaacaaccg ggtctccgag
     2401 gcggccgcgg tcctcgagca gggattgacc cggctgccgg acgcctttcc tctctattcc
     2461 cggctggctc atttttacat gagactcggc aagctccaag aagcgctgga catgagccgg
     2521 gcggccctcc ggatggatcc ccgctatttc gatgccctgg tcatcgccgc cgccgtggag
     2581 gacgtccggg gcaattgggc cgaatccgtc aaatattaca aaacggccct ggacgtcgaa
     2641 cccgaaaacc tgatggtcgg catgaagtat gcctatgccc tggcggcggc gggctccggg
     2701 gaggaggcgg cggccatcga cgaagccttg aagcgggaca atccgaagga tttccggatc
     2761 tatttggacc tgggcgtcat at
//
LOCUS       DAOOXH010000183         2764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_45102_length_2764_cov_8.214101, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000183 DAOOXH010000000
VERSION     DAOOXH010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45102_length_2764_cov_8.214101"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..2117)
                     /locus_tag="PKV64_11810"
     CDS             complement(42..2117)
                     /locus_tag="PKV64_11810"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPN17802.1"
                     /translation="MSILPLPGASSPHSNSLYKADPAFSRPLGLPGEPVQYLFRSGEG
                     SWHIASVGPSGVPETALLRLDASYEDLQLRRDAAGRLWLAGEEMTADGSITRAGVLEN
                     ETFGGSRTFGAPEGWNDGADLFFPAEGGLWAVWHHLSGKTEEILAEEAASGRRWRITP
                     EGAAALSPPRIAPDGLGGFWILWTGRIEGTYVVAARRWSGNSWSGEKRLFVNGEKPCL
                     QLDAALGRDGVLRAAWSAYDGSRYRVHAAGLADGGWTNIPLPVKKTGTGQSPRLVALD
                     DGRPAVLWADTGMTGTRLLGAVYDGSGIPAGPFVVSGKIPGPRFEAAGTDNGLFVILP
                     GPEGIRTECHRTAALLARPFSSVGEARVLEEPSGVAGPEVSESKYIGFGDSITYGYIN
                     STEAPELGYIPRLDDKLDEVFGSTDVINEGYPGELTAHGLGRIEAVLAARQARYILVM
                     EGTNDVKLFNNAGPVEVILYNLKEICLRSRAFGTMPILSTVCPRNDWIWNFPKYRNRH
                     IALEAGIRALAPQIVVPFVDMEAVFNANGGADALISDGVHPNIEGYKLMTETWYAAIR
                     ALPFPPLHPRLLSKEGMIFQELPAPENRLQKGLEHARVTVVRQIGYLLFWQANPKTAD
                     ASSLQGYRVYRKKHGESADGYVRIGEVSGVCAFLDRDITAVETYDYVITAVAFNGVEG
                     APSETVSND"
     gene            2269..>2764
                     /locus_tag="PKV64_11815"
     CDS             2269..>2764
                     /locus_tag="PKV64_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17803.1"
                     /translation="MKRALIASACFFFLFATLQYGQTLQNEKLLYHAPEGVRWTQCAF
                     GPDGVLWVIWAPGTTNDDSGGAVWVLSYDGITVSEPLRLTAAATRANRPHISASPRGY
                     IIASWGIVDTDSTFLRIWDPHSKTWGETEVVCQGYGGNEPCAGMDKDGNIHVFFSDEA
                     GGKVY"
BASE COUNT          538 a          831 c          861 g          534 t
ORIGIN      
        1 gcacgacggt ggatcatcat cacatgttcc tccagtgttg atcaatcatt gctcacggtt
       61 tcggaaggag cgccttccac gccgttgaac gcgaccgcgg tgatgacgta atcgtatgtt
      121 tccaccgccg tgatgtcgcg gtcgaggaaa gcgcatacgc ccgagacttc gccgatccga
      181 acgtacccgt cggcgctttc gccgtgcttc ttccgataaa cgcgataccc ctggagcgag
      241 gaggcatcgg ccgttttcgg attggcctgc cagaaaagca ggtaaccgat ttgacggacg
      301 acggttaccc tagcatgttc caagcctttt tgcaaccggt tttcgggcgc ggggagctcc
      361 tggaaaatca tcccttcctt ggaaagaagg cggggatgca agggcggaaa gggcagggcc
      421 cggatggccg cgtaccacgt ttcggtcatg agcttgtagc cctcgatgtt cggatggacg
      481 ccgtcggaaa tcagcgcgtc ggccccgccg ttggcgttga aaacagcctc catgtcgacg
      541 aaggggacaa cgatctgggg ggccagggcc cggatccccg cttccagggc gatgtgccga
      601 ttccgatact tggggaaatt ccagatccag tcgttgcgcg gacagaccgt ggacaggatc
      661 ggcatggttc cgaaggcgcg gcttcgcagg cagatttcct tgaggttata gaggatgact
      721 tcgaccgggc cggcgttatt gaagagcttg acgtcgttgg tcccttccat gacgaggatg
      781 taccgggcct ggcgggcggc caggacagcc tcgatccggc cgagcccatg ggccgtgagc
      841 tcccccgggt atccttcgtt gatgacgtcc gtggacccga aaacctcgtc gagcttgtcg
      901 tccagccggg ggatgtatcc cagctcgggg gcttccgtcg agttgatgta gccgtaggtg
      961 atgctgtcgc cgaaaccgat gtatttcgac tcgctgacct ccggtccggc gacgcccgaa
     1021 ggctcttcga ggacgcgggc ttctcccacg ctggaaaacg ggcgggcgag aagggcggcc
     1081 gtccggtgac attccgtccg gattccctcc ggaccgggaa ggatcacgaa gaggccgttg
     1141 tctgttccgg ccgcctcgaa ccgcggcccc gggattttcc cggacacgac gaacggcccg
     1201 gccgggattc cgcttccgtc gtaaaccgcg ccgagaagac gcgttccggt catccccgtg
     1261 tcggcccaga gaacggccgg ccggccgtcg tcgagggcga ccaagcgggg actttgtccg
     1321 gtccccgttt ttttgaccgg caaggggata ttcgtccatc cgccgtcggc gagtccggca
     1381 gcgtggacgc ggtagcgcga gccgtcatag gccgaccagg cggcccggag gacgccgtcc
     1441 cggcccaagg cggcgtcgag ctgaagacag ggtttttcgc cgtttacgaa aagacgcttt
     1501 tccccggacc acgaattccc gctccaacgc ctggcggcga cgacgtaggt cccttcgatc
     1561 cgcccggtcc atagaatcca aaatccgccg aggccgtcgg gcgcgatccg gggcggagac
     1621 aaagcggcgg ctccctccgg cgtgattctc catctccggc cggaagcggc ctcctcggcc
     1681 agaatctcct cggtttttcc agacaggtga tgccagacgg cccacaatcc cccttccgcg
     1741 gggaaaaaaa gatccgctcc gtcgttccag ccttccggag cgccgaaggt ccgcgaaccg
     1801 ccgaaggttt cattttccag aaccccggct ctggtgatgc ttccgtcggc ggtcatctcc
     1861 tctcccgcca gccagagacg gccggcggcg tcgcggcgca attgaagatc ctcataggac
     1921 gcgtccagac ggagaagggc cgtttccgga acgcccgacg gtccgacgga agcgatatgc
     1981 caggatcctt ccccggatcg aaaaagatat tgaaccggct cgccgggaag cccaagaggg
     2041 cgggagaacg ccggatcggc tttatacaga gagttgctat gcggggagga cgcgcccggc
     2101 aggggaagaa tcgacataag ccccgccagc ccgaccccta atgtccggag aaacgtttta
     2161 aacgcgtttt ccataacaat gactataaca tatttatata acgggttgac aagcatctgt
     2221 ttccttggca tgataaatcg ctatacctgg cacaaggagc cgaagacgat gaagagagcc
     2281 ttgattgcaa gcgcgtgttt tttcttcctt ttcgccacgc ttcaatacgg ccaaaccctg
     2341 cagaatgaaa aactgctgta ccatgccccg gaaggcgtgc gctggactca atgtgccttc
     2401 ggccccgacg gcgttctgtg ggtgatttgg gctcccggaa ccaccaacga cgacagcggc
     2461 ggcgcggttt gggttctcag ttacgatggg atcacggttt ccgaaccgct gcggttgacg
     2521 gcggcggcga cgcgggccaa ccgtccgcac atctcagcct cccctagggg ctatattatc
     2581 gctagttggg gcatcgtcga cacggattcg acttttctcc ggatttggga tccccattcc
     2641 aaaacgtggg gagagaccga agtcgtttgc caggggtacg ggggaaatga accttgcgcc
     2701 ggcatggata aagacgggaa catccatgtc tttttctccg atgaagccgg agggaaagtc
     2761 tatg
//
LOCUS       DAOOXH010000184         2711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_46339_length_2711_cov_7.387048, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000184 DAOOXH010000000
VERSION     DAOOXH010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46339_length_2711_cov_7.387048"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PKV64_11820"
     CDS             <1..489
                     /locus_tag="PKV64_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPN17804.1"
                     /translation="PEKNPLFVPPVSTFEPTKKDANVPNINTIPGEDVFYMDCRILPH
                     YRLEDVEGRVQAACRSIEKKFGVTITTESMQRAKAAPPTPPGSAVVEALKRAIQAVYQ
                     KESRPIGIGGGTVAAYFRREGFEAACWSKIDDTAHQPNEYCLIDNMVGDAKVFAHIMM
                     QE"
     gene            complement(500..889)
                     /locus_tag="PKV64_11825"
     CDS             complement(500..889)
                     /locus_tag="PKV64_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17805.1"
                     /translation="MGNRKGERIGWICGWLGAFLWVVVLAVIFFVQGRAAEAAVGLGI
                     AALAVVLVFALAPWRHPARAYWKIMIPLYLVLFASAAWVIRVYGGLRGIRLPVWNLLF
                     FVPILIPLGLLGRRKWRDFDPASRSDH"
     gene            1019..2386
                     /locus_tag="PKV64_11830"
     CDS             1019..2386
                     /locus_tag="PKV64_11830"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPN17806.1"
                     /translation="MDSIGQNPSSAPSVSELPRVLGVFSIIGIVVGTMIGSGIFINPP
                     NVAKVVGTPEIMMAVWIVGGLLTLFGALSLSELGAAYSHAGGIYVYLREAYGPMVAFL
                     FGWSLFIVIESGTVASLAVGFSTKYLPYFVDLSPWQSKIVSIGLILALAAVNYRGVRK
                     GAGLMNFLMVIKFVALIGMCVLVFLLVKGTPRNFLSAPSGAGPAGNVLSNFGVALIAA
                     LWAYKGWETSSYSAGEIRNAQKKLPVGLLLGIAAVVFLYIAANLAYLYVFPAPVMAES
                     PHIAADVMQAAVGPVGASIIALIILMSITGTCNGHLLTAPRVFYAMAKDGFFFRSMAR
                     VHPRYKTPHVAIVVLAAWGAVICTSGTFEQLFTFVMFGYWIFMGLAVGGVIVLRKKRP
                     GLPRPYKAWGYPVTPLLFILAMAFLTVNSLVRTFWNSFAGLGLIAAGIPVYFFWKARG
                     KKARA"
     gene            2412..>2711
                     /locus_tag="PKV64_11835"
     CDS             2412..>2711
                     /locus_tag="PKV64_11835"
                     /inference="COORDINATES: protein motif:HMM:NF015573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HPN17807.1"
                     /translation="MLNLDLIQTVAFAGVVLFIGYALCRWIRPLSRYNIPAPVVGGLL
                     VAVAVLIAHQNGATLISFDTTLRDPLMIAFFTTIGFGASFSLLKVGGPQVVLFFVI"
BASE COUNT          482 a          917 c          774 g          538 t
ORIGIN      
        1 cccgagaaaa atccgctctt cgtgccgccc gtctccacgt tcgagccgac taagaaggac
       61 gccaatgtcc cgaacatcaa cacgatcccg ggcgaggacg ttttttatat ggattgccgg
      121 attctccccc attaccggct cgaggacgtc gagggccgcg tccaggcggc ctgccggtcg
      181 atcgagaaaa agttcggcgt gacgatcacc accgaatcca tgcagagggc caaagccgcc
      241 ccccccacgc cgcccggatc agccgtggtc gaagcgctga aacgcgcgat ccaggcggtc
      301 taccagaagg aaagccgccc tatcggaatc ggcggcggga ctgtggccgc ctatttccgc
      361 cgggaaggat tcgaagccgc ctgctggtcg aaaatcgacg acaccgccca ccagcccaac
      421 gaatactgcc tcatcgacaa catggtcggc gacgccaagg ttttcgccca catcatgatg
      481 caggaatgag gccgggatct caatgatccg atcgggaggc cggatcgaag tccctccatt
      541 ttcttcggcc gaggaggccg agcggaatca ggatcgggac aaagaacagc agattccaaa
      601 ccggaaggcg tattcccctt aagccgccgt aaacccgaat gacccaggcg gccgaagcga
      661 acaagaccag gtaaagcggg atcatgattt tccaatacgc ccgggccgga tgccgccacg
      721 gagcgagggc gaagacaagg acgacggcga gggccgcgat ccccaggccg acggccgcct
      781 cggccgctcg gccctgaacg aaaaagatca cggcgaggac aacgacccag agaaaggcgc
      841 ccagccagcc gcagatccag ccgatcctct ctccctttcg gtttcccatg ttctcctcct
      901 cgaacgaacg gccccttggc cgtttttcca agataatgcc ccccgcggcg ccctgtcaac
      961 cgcggcccgg gagccggtcc gcattgacag cccttcgtta atccatttta atacgcccat
     1021 ggattccatc ggacaaaatc cttcctccgc cccttccgtt tccgagctgc cccgcgttct
     1081 gggggttttc agcatcatcg gcatcgtcgt cgggacgatg atcggttcgg gcatcttcat
     1141 caatccgccc aacgtggcca aggtcgtggg aacgccggag atcatgatgg ccgtctggat
     1201 cgtcggcggc cttctgactc ttttcggggc gctgtctctg tccgagctgg gggccgccta
     1261 ctcccacgcc ggcggaatct acgtctatct ccgcgaagcg tacggaccga tggtcgcctt
     1321 cctcttcggc tggtcgctgt tcatcgtcat cgaatcaggg acggtggcct ccctggccgt
     1381 gggcttctcg accaagtatc tcccctattt cgtcgacctg tcgccgtggc agtccaagat
     1441 cgtctcgatc ggactgatcc tggccctggc cgccgtgaat taccggggcg tccgcaaggg
     1501 cgcgggcctg atgaacttcc tgatggtcat caagttcgtc gccctgatcg gcatgtgcgt
     1561 ccttgtcttc ctcctggtca agggaacgcc gcggaatttt ctctcggctc cctccggagc
     1621 ggggccggcc ggaaacgtcc tgagcaattt cggcgtcgcc ctgatcgccg ccttgtgggc
     1681 gtacaaggga tgggagacga gctcgtacag cgccggggaa atccgcaacg ctcaaaagaa
     1741 gctgccggtc ggacttcttc tcggcatcgc cgcggtcgtc tttctctata tcgcggccaa
     1801 cctcgcctat ctctacgtct tccccgcgcc cgtcatggcc gagtcgccgc acatcgccgc
     1861 cgacgtcatg caggccgcgg tcggaccggt cggggcgtcg atcatcgccc tgatcatcct
     1921 gatgtccatc accgggacct gcaacggcca cctgttgacc gccccccggg tcttttacgc
     1981 catggctaaa gacggatttt ttttccggtc catggcccgg gtccacccgc gatacaaaac
     2041 tccccacgtg gcgatcgtcg tcctggccgc ctggggagcc gttatctgca ccagcgggac
     2101 gttcgaacag cttttcacgt tcgtcatgtt cggatactgg atcttcatgg gcctggccgt
     2161 gggcggcgtg atcgttctcc gaaaaaagag gcccggcctg ccccggccct acaaagcctg
     2221 gggatatccg gtcacgcccc tcctgttcat cctggcgatg gccttcctga ccgtcaattc
     2281 gctcgtccgg acgttttgga actcgttcgc cggcttgggc ctcatcgcgg ccggaattcc
     2341 agtctatttc ttctggaaag cccgggggaa gaaagcccgg gcctgatcgg cgggccgaaa
     2401 ggaatcatcg gatgctgaat ctcgacctca tccagaccgt cgccttcgcc ggcgtcgtcc
     2461 tcttcatcgg atacgccctc tgccgctgga tccgccctct ttcccggtac aacatcccgg
     2521 ccccggtcgt gggcggcctg ctggtggccg tcgccgtcct gatcgcccac cagaacgggg
     2581 cgacgctgat ctcgttcgac acgaccctcc gcgatccgct gatgatcgcc tttttcacga
     2641 cgatcgggtt cggagccagc ttctccctgc tcaaggtcgg cgggccgcaa gtcgttctgt
     2701 tcttcgtcat c
//
LOCUS       DAOOXH010000185         2699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_46628_length_2699_cov_5.374433, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000185 DAOOXH010000000
VERSION     DAOOXH010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46628_length_2699_cov_5.374433"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="PKV64_11840"
     CDS             <1..1258
                     /locus_tag="PKV64_11840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPN17808.1"
                     /translation="KFIALFWAKRDPSPLDKANEFKDEWYARLEYVNKTYSKGSIAKG
                     WHTDMGRVYMMFGPPAQIRGGGSGQVRPESAGGSQIMGPPETWVYQPMPALGLNSEFA
                     VTFLNQQFGYDLDEMTPQPIRHALDIFSKVVIFNPDLKELPMYKFSLDESSAEAKLIN
                     EFIAGGQEINQIPLEWTPIYTRAMSGSTYVSFLVRIDAQNLDRKKLREMTFFGRLQGE
                     GEEVQEFLQPLKLDQDKSDKMLAVFGFPAKAGKSVLYLGAEDKDKELHTLVKADLDVQ
                     NFWNDELNTSSLILSSQVISKPREEAGAEFSPFVTTDYKAAPRWGNAFKPSEFLSVLY
                     QVYNAKTQDGALDLSIDYFIISDEAGYRLNPQTVKTKVEENMAVACGTEVPLSPLKPG
                     KYTFKIRVTDKIAGKNVEKTAPFVVE"
     gene            1269..1880
                     /locus_tag="PKV64_11845"
     CDS             1269..1880
                     /locus_tag="PKV64_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17809.1"
                     /translation="MFRSLCGFVAAALVITAAACAPVVPEDPNTIEITKVGSYSYMRQ
                     SRDLAIVVDVEMARKRTEEAYFPLGIKIANKRLGQVIVDRETLVLVDENGRIYPMPGV
                     VELERNYDKLVPDHKFQSQTGIMGDTLLTGFSYYQKADSRFFPQVQGGGRVIDSVKIR
                     AHGYLEDMIYFPMPEGGIKGRMLRLRLDVYELEKPFEIVFPVQ"
     gene            complement(1914..2372)
                     /locus_tag="PKV64_11850"
     CDS             complement(1914..2372)
                     /locus_tag="PKV64_11850"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPN17810.1"
                     /translation="MFDEDDGALSFRLDRESPYSFHCRRCGVCCANKRLMLSPSERKR
                     LAVFLGVPEERFTTDFLDSESGELLSASNGDCVFLEAGLCRVHPVRPLVCRLYPLGLL
                     RDETGRETFGVMPLHPDCLGFLGTDGTVGGDLNAQGSDVLLEQEKESRPD"
     gene            complement(2377..>2699)
                     /locus_tag="PKV64_11855"
     CDS             complement(2377..>2699)
                     /locus_tag="PKV64_11855"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17811.1"
                     /translation="VFSNIRYWATGKIIAPKARTDDGLLDLCLIKPCRFLRLAEIYRL
                     AGKGRHGRLRQVEFRQAGVFRLRSEVPMEIQADGDILGTNDGRTRFNDLEIGILPAAL
                     QIIG"
BASE COUNT          563 a          819 c          833 g          484 t
ORIGIN      
        1 caagttcatc gccttgttct gggccaagcg cgatccgtcg cccctcgaca aggcgaacga
       61 gttcaaggac gaatggtacg ctcgtctgga atacgtcaac aagacgtatt cgaagggctc
      121 gatcgccaag gggtggcaca cggacatggg ccgggtctac atgatgttcg gccctccggc
      181 ccagatccgc ggaggcggat cgggccaagt gagaccggaa tcggccggcg gctcccagat
      241 catgggaccc ccggaaacct gggtgtatca gcccatgccc gccctcggat tgaacagcga
      301 gttcgcggtc actttcctga accagcagtt cggctacgac ctggacgaaa tgacgcccca
      361 acccatccgc catgccttgg acatcttttc caaagtcgtc attttcaatc ccgacctcaa
      421 agagcttccc atgtacaagt tttccctgga tgaaagctcg gccgaggcga agctgatcaa
      481 tgaattcatc gccggcggcc aggaaatcaa ccagattccc ctggaatgga cgccgattta
      541 cacccgggcg atgagcggca gcacgtatgt gtcgttcctg gtccggatcg atgcccagaa
      601 cctcgaccgg aaaaaactcc gggaaatgac gtttttcgga cgtctccagg gggaaggcga
      661 ggaagttcag gaattcctcc aaccgctcaa gctcgatcag gacaagtccg acaagatgct
      721 ggcggttttc ggctttccgg ccaaggccgg caaatcggtc ctttatctcg gcgccgagga
      781 taaggacaag gagcttcata ccctggtgaa ggccgacctg gacgttcaga atttctggaa
      841 cgacgagctg aacacaagct cgctcatcct cagctctcag gtcatttcca agcccaggga
      901 agaggccggg gcggaattca gcccctttgt cacgaccgac tacaaggccg ccccgcgctg
      961 gggcaacgcc ttcaagccga gcgagtttct gagcgtcctc tatcaggttt acaacgccaa
     1021 aacccaggac ggcgcgctcg acctgtcgat cgattacttc atcatctccg acgaagccgg
     1081 ctaccggctg aatccccaga cggtcaagac caaggtcgag gaaaacatgg ccgtggcctg
     1141 cggaacggag gtccccctct cgccgctcaa gccgggcaag tacactttca agatcagggt
     1201 cacggacaag atcgccggca agaacgtgga gaagaccgcg ccgttcgtgg tcgaataacg
     1261 ggccggccat gttccgttcc ttgtgcggct ttgtcgccgc ggccctggtg atcacggccg
     1321 cggcctgcgc tccggtggtt ccggaggatc cgaacacgat cgaaatcacg aaggtcggga
     1381 gctattccta catgcggcag agccgcgacc tggccatcgt cgtcgatgtg gagatggccc
     1441 ggaagcggac cgaggaggcg tatttccccc tggggatcaa gatcgccaac aagcgcctcg
     1501 gccaggtgat cgtcgaccgg gagaccctgg tcctcgtgga tgagaacggc cggatttacc
     1561 ccatgccggg cgtcgtcgag cttgagcgga attacgacaa gctcgttccg gatcataagt
     1621 tccagagcca gaccgggatc atgggcgaca cgctcttgac gggcttctcc tactaccaga
     1681 aagccgacag ccgctttttc ccccaggtcc agggcggcgg ccgggtgatc gattcggtga
     1741 aaatccgggc gcacgggtac ctggaagaca tgatttattt cccgatgccc gaggggggga
     1801 tcaagggaag gatgctccgt ctccggctgg acgtatacga gctcgaaaag ccgttcgaga
     1861 tcgtctttcc cgttcagtag ggccttcggg cggatcgccg cagccggccg gactcagtcc
     1921 ggccggcttt ctttttcttg ctcgaggaga acgtcggatc cttgggcgtt caggtcgccg
     1981 ccgacggtcc cgtccgttcc caggaatccc aagcagtcgg gatgaagggg catgaccccg
     2041 aacgtctcgc ggcccgtttc gtcccggaga agacccagcg ggtaaagacg gcagacgagc
     2101 ggccggaccg gatgaacgcg gcagaggccg gcctccagga aaacgcagtc tccgttggag
     2161 gcggagagca gctcccccga ttccgagtcg agaaaatccg tcgtgaagcg ttcttccggc
     2221 accccgagga agacggccag gcgcttccgc tcggaggggg aaagcatcag gcgcttgttg
     2281 gcgcagcaga cgccgcagcg ccggcagtgg aaggaatagg ggctttcccg gtccagccgg
     2341 aacgacagcg cgccgtcgtc ctcgtcgaac aacccctcac ccgatgattt gaagggcggc
     2401 gggaaggatc ccgatctcca agtcgttgaa tctcgtccgg ccgtcgttcg tcccgaggat
     2461 gtctccgtcg gcctggatct ccatgggaac ctcggaccgg agacggaaaa caccggcctg
     2521 ccggaactcg acttggcgga gccgtccatg ccgccctttc ccggccaggc ggtatatctc
     2581 ggccaggcgg agaaaacggc agggcttgat gaggcaaaga tcgagaaggc cgtcgtcggt
     2641 tcgagctttg ggggcgatga ttttccccgt cgcccagtag cggatgttgg aaaacacgg
//
LOCUS       DAOOXH010000186         2697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_46689_length_2697_cov_7.291067, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000186 DAOOXH010000000
VERSION     DAOOXH010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46689_length_2697_cov_7.291067"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..884)
                     /gene="rsmD"
                     /locus_tag="PKV64_11860"
     CDS             complement(249..884)
                     /gene="rsmD"
                     /locus_tag="PKV64_11860"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPN17812.1"
                     /translation="MGRPSAGPPKSAEPRGRSPSLLLKREGGMIRVISGLYKGKRLRK
                     VPSPLVRPYPDKLKTALFNILRDDLPGLKVLDGFAGTGSIGIEALSNGAAFAVFVEIL
                     PAAVGTLRHNLEKLEAGDRSRIMAMEFNRAVIQLAREGARFDLIFLDPPYRLLEERNP
                     LKVIRKRGLLTPGGRIVLRRHFKTRFEAGDFDLERQVRLGDDTLCFYRGPA"
     gene            complement(815..1411)
                     /locus_tag="PKV64_11865"
     CDS             complement(815..1411)
                     /locus_tag="PKV64_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17813.1"
                     /translation="MNGKRRRRPRIILPAAGLILLAAAAARAGIEITAEMRKDAARLQ
                     SALDLIERNRAARAALPMRSLAFSEKELNAWVAVLIESDRDGILRELALKLFEENRVE
                     GKAFVDAGGVSLPFGLKPRLNIFFSGRVTVLDGAAKIALDKLFFEGQPVSVVLLDAVI
                     AAAAAMNKSGASSINDWVALPPGLRNLRSREGVLLLYY"
     gene            complement(1526..2314)
                     /locus_tag="PKV64_11870"
     CDS             complement(1526..2314)
                     /locus_tag="PKV64_11870"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPN17814.1"
                     /translation="MKAGKSLKKCVFLFFIVAWTVGAAAAQTVERGHLVGNIFARDGK
                     TPVAGAVVRLKNITSGAVSAADPTDAQGYFRLENLSKGIYQFGVTTPEGDFNSSELIG
                     ILANETTRISLALDVYDSAVQTAVQEVLREQAVEEGESRIGRVVRYNPATREAEVFIE
                     KGILQLDDRVRVKGLQTNFFQNVDKLGLAGESVKRLFAGQNGLMPTERSTEPGDGVYV
                     VCKKGVPPFFLTPCGIASVIAGTGLILSGVVEVPEKKDVSPFRK"
     gene            complement(2384..>2697)
                     /locus_tag="PKV64_11875"
     CDS             complement(2384..>2697)
                     /locus_tag="PKV64_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17815.1"
                     /translation="IEAGLTPRETWLGDSIWMAGEAFSKDLVLDEKIYRLELEFSDAA
                     AGPSILLSIVINGKILFEDFISSSVIDLEFPAALGRNTLNITPLNRFCRPIKMILIPA
                     E"
BASE COUNT          545 a          818 c          816 g          518 t
ORIGIN      
        1 tcttggccag cgccgccgag acttcattgg ccgtgtaacg cccgagaaac agcaccgtcc
       61 cccggcggtc tccgagcgaa ccgaagattt cgctctcatc gaccaagggg ctgttccaat
      121 ccgtcggagc gggttgatga ttcttcatcg tccttcctgt ttcggctgtt aaaaaatccc
      181 ttttcgattt taaatgattc aatccccgga ggcaagaatg gagaagaccg cgggaaacgc
      241 cgccggcgtc aggcgggccc gcggtagaaa cagagcgtat cgtcgcccag ccgaacctgc
      301 cgttccaagt cgaagtcccc ggcctcaaac cgggtcttga aatggcggcg gaggacgatc
      361 cggccgcccg gggtcagaag tccgcgcttg cggatgacct tgagggggtt gcgttcttcc
      421 aaaagccggt aaggcggatc aagaaaaatg aggtcgaacc gggcgccctc gcgggccaat
      481 tggatgaccg cccggttgaa ttccatggcc atgatccggg agcggtctcc ggcctcgagc
      541 ttctccagat tgtggcgaag cgtcccgacg gccgccggca ggatctcgac aaagacggcg
      601 aacgccgccc cgttgctcag ggcctcgatg ccgatcgatc ccgtcccggc gaatccgtcg
      661 aggactttca gaccgggcaa atcgtcgcgg aggatgttga acagcgccgt tttcagcttg
      721 tcgggataag gccggaccag ggggctgggg accttgcgca ggcgctttcc cttgtagagg
      781 ccgctgataa ccctgatcat tcccccctcc cgtttcagta ataaagaagg agaacgccct
      841 cgcggctccg cagatttcgg aggcccggcg gaagggcgac ccaatcgttg atcgaagagg
      901 ccccggattt gttcatggcg gccgccgcgg cgatgaccgc gtccagcagg acgacggaca
      961 ccggctgtcc ctcgaagaag agcttgtcga gggcgatctt ggcggccccg tccaaaacgg
     1021 tcacccgccc ggaaaaaaag atgttcaagc gcggcttgag gccgaaagga agcgagactc
     1081 cgcccgcgtc gacgaacgcc ttgccctcga cccggttttc ctcgaatagc ttgagagcca
     1141 gctcgcggag gatgccgtcg cggtcggatt cgatcaggac ggccacccag gcgttcagct
     1201 ccttctcgga aaaggccagg cttcgcatcg ggagggcggc ccgggccgcc cggttccgct
     1261 cgatcagatc gagggcggac tgaaggcggg ccgcgtcttt tctcatctcg gccgtgatct
     1321 cgattccggc ccgggccgcg gccgcggcca ggagaatcaa gcccgccgcc ggcaggatga
     1381 tccgcggccg gcgacgacgt tttccgttca tggggctatt ctatcaaatc ggcggcttcc
     1441 aggggaaaac tccaggcaaa aaaaatcccg ccgtcccgcc ggtcgatcga acggcggggc
     1501 ggcgggacga cgtccgtccg cctggttact tccggaaggg agagacgtct tttttttccg
     1561 gaacctcgac gacgccggac aggatcaaac cggttccggc gatgacgctg gcgatgccgc
     1621 aaggggttaa aaagaacggc gggacgccct ttttgcagac gacataaacg ccgtcgcccg
     1681 gttcggtcga gcgttccgtc ggcatcaagc cgttctggcc ggcgaatagg cgcttgacgg
     1741 attcccccgc cagcccgagc ttatccacgt tttgaaagaa attcgtctgc aggcccttga
     1801 cccggacccg gtcgtcgagc tggaggatgc ccttctcgat gaagacctcg gcttccctgg
     1861 tcgccggatt gtaccggacg acccgtccta tgcggctctc cccctcctcg acggcttgtt
     1921 ccctcaggac ttcttggacc gcggtctgaa cggcggagtc gtagacgtcg agcgccagag
     1981 agatcctcgt cgtctcgtta gccaggatgc cgatcagctc gctcgagttg aaatcgccct
     2041 cgggagtcgt gacaccgaac tggtagattc ccttgctcag gttttccaag cggaaatagc
     2101 cctgggcgtc ggtcggatcg gcggccgaga cggccccgct ggtgatgttc ttcaagcgga
     2161 cgaccgcgcc ggcgacgggc gttttgccgt cccgggcgaa gatgttcccg acaagatgac
     2221 cccgctcgac cgtctgagcg gcggccgcgc cgacggtcca ggcgacgata aaaaaaagaa
     2281 aaacgcactt tttaagggac tttcctgctt tcattgcttc ctcccaaacc ttagcattta
     2341 aattatatgt acgaaatttt tcaattgtca agcgcggtca gaattattca gccgggatca
     2401 ggatcatctt gatcggccgg cagaagcggt tgaggggcgt gatattcaaa gtatttcgac
     2461 ctaaagccgc cggaaactcg aggtcgatga cgcttgagga aatgaagtcc tcaaaaagaa
     2521 tttttccgtt gatcacgatc gaaagaagga tcgacggacc ggcggccgcg tcggaaaatt
     2581 ccagctcaag gcgatatatc ttttcgtcaa gaacaaggtc ctttgaaaaa gcctcccccg
     2641 ccatccatat cgaatctccc agccaggttt ctcggggggt caaaccggcc tcgatct
//
LOCUS       DAOOXH010000187         2665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_47516_length_2665_cov_7.821839, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000187 DAOOXH010000000
VERSION     DAOOXH010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47516_length_2665_cov_7.821839"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PKV64_11880"
     CDS             <1..428
                     /locus_tag="PKV64_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17816.1"
                     /translation="QPYRICGLLQAAPQTPNEKIGEMLDLLFDLLRKAGRDGFTPAEV
                     ESSRNYIQGQFPPTLETNGAKANAYVQLAYFNLGFDYYDRYLAGVKTTSPEAAKAAAA
                     RLIPANDYVLVVVGKADEIRDQLKKFGAWTEKKISDPGF"
     gene            575..1276
                     /locus_tag="PKV64_11885"
     CDS             575..1276
                     /locus_tag="PKV64_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17817.1"
                     /translation="MTRAMKTLSSALILTFVLAAAAGAAPPEFQFGLAFNPALPQGGF
                     HDVLGRTIWGGSFQFAFRPSGSPVLLGTSLGFGIYGFDSWQDWLGILDPDVLVDIRTT
                     NAVLAWNVFLRLQPARGLIRPYLDVFAGLHLLTTDTRIGDGDDDGSGGFSVNNAADTA
                     FACGAGAGVMLPVVRFVHRDGRPAAAINLDLGARYAFGGRADYLVKTDRHGVFDERTS
                     RTDLLILNAGLSFCF"
     gene            1553..>2665
                     /locus_tag="PKV64_11890"
     CDS             1553..>2665
                     /locus_tag="PKV64_11890"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPN17818.1"
                     /translation="MKYKPALFAVLTAVLLTLGGAMGHGVQARAANPDDPAGCPTCLD
                     FNPRLEACTVILVGKNASTDGSVMTTHTCDCGLCDWTFRRVPAKDHQPGEMRRIFYSD
                     QFGTWPPDLGLKWDRVPVDGFTGLEIPEVPHTYGYILGAFGYMNDKQVSIGESTIGNV
                     EKLENNTPAPKFDITMLTIMAMERGGTAREAIQVMGSLAEKYGYGHTDSGEMLAVADP
                     NEVWLFEIFPVGALWTPASGKPGAVWCAQRVPDDHVSVCPNESRIGEIDLNNKDYFMA
                     SPNVIQLAIDQKLFDPKSGKPFNWKKAYSPNDVSAASTAGARVRLWRFFNLVAPSLNL
                     GPQTNNMDFPFSVKPEKKLSVQDVMNMTRDKCEGTVF"
BASE COUNT          512 a          891 c          750 g          512 t
ORIGIN      
        1 tccaacccta ccggatctgc ggcttgctcc aagccgcccc gcagaccccg aacgaaaaaa
       61 tcggcgagat gctcgacctt ctcttcgacc tgctccggaa agccggccgg gacggattca
      121 cgcccgcgga ggtcgaaagc tcgcgcaatt atatccaggg gcaattcccg ccgaccctcg
      181 agacgaacgg cgccaaggcc aacgcgtacg tccaactggc ttatttcaac ctcgggttcg
      241 attactacga ccggtatttg gccggcgtca agacgacgag cccggaggcg gccaaggcgg
      301 cggcggcccg gctgattcca gcgaacgatt acgtcctggt cgtcgtcggc aaggccgacg
      361 agatccggga ccagttgaaa aaattcgggg cctggacgga aaagaagatc tccgatcccg
      421 gattctgagc ctctaaggag acgccatacg gattcccctc attttccgga tcgttctccc
      481 gggtttcgga agaaacaaaa agaggttcaa aggtattgtg tctcctcaat tcttgctttt
      541 cggggaacgg ccccggggat aaacaggtga acaaatgacg cgcgccatga aaacactctc
      601 ctccgccttg atcttaacgt tcgtcctcgc cgccgcggcc ggagccgcgc cgcccgaatt
      661 ccaattcgga ctggccttca atcccgccct tccgcagggc ggattccatg acgtcctcgg
      721 acggacgatc tggggcggct cgttccagtt tgccttccgg ccctcgggca gtccggttct
      781 cctcggaaca tcgctgggct ttggaatcta cggcttcgat tcctggcagg attggctggg
      841 gatcctcgat cccgacgtgc tcgtcgacat ccggacgacc aacgccgtcc tggcctggaa
      901 cgtgttcctt cgtctccagc cggcgcgcgg cctcattcga ccctacctcg acgtcttcgc
      961 cgggctgcat cttctgacca cggatacgcg gatcggagac ggggacgacg acggctccgg
     1021 cggcttcagc gtcaataacg ccgcggacac ggccttcgcc tgcggcgccg gcgcgggcgt
     1081 catgcttccg gtcgtccgtt ttgttcaccg ggacggccgt ccggccgcgg ccatcaacct
     1141 ggatctcgga gcccgttatg ccttcggcgg ccgggcggat tacttggtca aaacggaccg
     1201 ccacggcgtt ttcgacgagc ggacgtcgcg caccgacctg ctcatcctca acgccgggct
     1261 gtccttctgc ttctgacccg cggctgttca aaagggagac accataccca tccccccgat
     1321 tttccggaac gttcccccga gtttcagaaa aaagaaaaat ggagttcaca aggattgtgt
     1381 ttccgaattt cttcagaggc ttgtttttca tttgatctcc gcgtttcctc ctcgcccgga
     1441 acggcaagga ttcgatggcc ttgacatcgg aggaaccgga agaggcgaaa ttggattcat
     1501 aggtattgtg tctcccaatt tcttccgtta tagtatccgt ccggaggaat ccatgaaata
     1561 caaacccgcc cttttcgccg ttttgaccgc cgtcctcttg acgctcggcg gagcgatggg
     1621 acacggagtg caggcccggg ccgcgaatcc ggatgatccg gccggctgcc cgacctgcct
     1681 ggacttcaat ccccgtctcg aagcctgtac cgtcattctg gtcggcaaga acgcctcgac
     1741 cgacgggtcg gttatgacga cccacacctg cgactgcggc ctgtgcgact ggaccttccg
     1801 ccgcgttccg gccaaggacc accagccggg cgaaatgcgc cggattttct actccgatca
     1861 gttcgggacc tggccgcccg acctcggcct caagtgggac cgcgtccccg tggacggctt
     1921 caccggcctg gaaattcccg aagtccccca tacgtacggc tacatcctgg gcgccttcgg
     1981 ctacatgaac gacaagcagg tctccatcgg cgagtcgacc atcgggaacg tggagaagct
     2041 ggagaacaac accccggccc cgaaattcga catcaccatg ctgaccatca tggccatgga
     2101 gcgcggcggg accgcccgcg aggccatcca ggtcatgggc tcgctggccg agaaatacgg
     2161 ctacgggcat accgactccg gtgaaatgct ggccgtggcc gatcccaacg aagtctggct
     2221 gttcgaaatc ttccctgtcg gcgccctgtg gacgccggcc agcggcaaac ccggcgcggt
     2281 ctggtgcgcc cagcgcgtcc ccgacgacca cgtttcggtc tgccccaacg agtcccggat
     2341 cggcgagatc gacctgaaca acaaggatta cttcatggcc tcgccgaacg tcatccagct
     2401 ggcgatcgac cagaagcttt tcgatcccaa gagcggcaag cctttcaact ggaaaaaggc
     2461 ttactccccc aacgacgtca gcgccgcctc gaccgccgga gcccgcgtcc ggctgtggcg
     2521 gttcttcaac ctcgtcgctc cttccctcaa cctcggcccc cagaccaaca acatggactt
     2581 ccccttctcg gtcaagcccg agaagaagct ctccgtccag gacgtcatga acatgacccg
     2641 ggacaagtgc gaaggcacgg tcttc
//
LOCUS       DAOOXH010000188         2640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_48132_length_2640_cov_6.340039, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000188 DAOOXH010000000
VERSION     DAOOXH010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48132_length_2640_cov_6.340039"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PKV64_11895"
     CDS             complement(<1..326)
                     /locus_tag="PKV64_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17819.1"
                     /translation="MKNAFRLLVALVEGTIVTARHFFSNLWFHFLRLIGLKKYRPGAV
                     TIQYPEETKPLPLRYRAAHRLMKRPGGKPRCVACLLCATVCPSECIVVEAAGDRDPEI
                     QKTAGR"
     gene            complement(323..1399)
                     /locus_tag="PKV64_11900"
     CDS             complement(323..1399)
                     /locus_tag="PKV64_11900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPN17820.1"
                     /translation="MSEISVLLVKIAVALGWLLLLALFLTWVERKQSAVMQDRIGANR
                     ARVLGLRMFGLFQPFADAIKMIFKEDFVPPFADKVLHTLAPFVSFFFVAAAVAVVPFG
                     PAVRIGGTPVLLQVYDGTAGLLFLLAMLSLGVYGILLSGWSSRNRWGLIGGLRGAAQL
                     VSYEVGLGLSLVGLIMVYPSLRLSEIVAYQGGLILGFLPRWGVFLQPLGFLVFFVAAV
                     AETKRVPFDLPEGESEIVGFNAEYGGLKWGLFMLTDFMEIIVVAALAATLFFGGWQAP
                     WLGPDGVRWPWGGHWAMAGGVVLFLQVLSFQLKVAFFCWLQILIRWTYPRIRYDQLMD
                     LGWKLLVPLGLLNVLGTGLVMSLR"
     gene            complement(1396..>2640)
                     /locus_tag="PKV64_11905"
     CDS             complement(1396..>2640)
                     /locus_tag="PKV64_11905"
                     /inference="COORDINATES: protein motif:HMM:NF016750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17821.1"
                     /translation="ARRNVLELLLADHQVDCPICDKAGECRLQDYYRDYGLFPSDLRE
                     PKTRREKLLKIGERLILDRERCVLCTRCVRFLRDVAKTGELGVFGRGGGSEIGFIEGA
                     AVATPYAGNLVDLCPVGAITDAAFRFRTRPWFLEPKPSICPFCGRGCGIEVDVHPGFA
                     RHPETGGIFRVRPRPDATPAGPWICDVGRYAWIEIESRRARAIVWNKGGGGEAVLSAE
                     KAFALLGAKIRALDETERTGRLALVVHSGLTNEEWDDVRMFRDGFHPRPLLRLADPPD
                     DGGDGFLRTPERTPNRRGALAAGGADLRPLDLDELRARADLLFVFAGVRGEVTPWILH
                     PEEWRSIRTKVLVSPVLTEADDLFDFVLPCASAFEKSGSYTNVEGRRREFGAARPVSE
                     GIRTEGGILRSLLEAVRRGKRE"
BASE COUNT          551 a          872 c          851 g          366 t
ORIGIN      
        1 aaccggcccg cggtcttctg gatttccggg tcgcggtccc cggccgcctc gacgacgatg
       61 cattccgaag ggcagacggt cgcgcagaga aggcaggcca cgcaccgcgg ctttccgccg
      121 ggccgcttca tcaagcggtg ggccgcccgg tatcgaaggg gaagcggctt cgtttcctcg
      181 ggatattgga tggtcaccgc cccgggccga tattttttca ggccgatcag ccggaggaaa
      241 tggaaccaaa gattggagaa aaaatggcgg gcggtgacga tcgtcccctc gacgagcgcc
      301 acgagaaggc ggaacgcgtt tttcatcgca ggctcatgac caagccggtt cccaagacgt
      361 tgagaagacc gagagggacg agcagcttcc accccagatc catcaattga tcataacgga
      421 tccgcggata ggtccagcgg atgagaatct gaagccagca gaagaaagcg accttgagtt
      481 ggaaggagag gacctggaga aagaggacga cgccgccggc catcgcccaa tgtccgcccc
      541 agggccagcg cacgccgtcg ggaccgagcc acggcgcctg ccagccgccg aaaaacagcg
      601 tcgcggccag ggcggccacg acgatgatct ccatgaaatc cgtcagcatg aaaaggcccc
      661 acttgaggcc gccgtattcg gcgttgaagc cgacgatctc ggactctcct tccggcaggt
      721 cgaacggcac gcgcttcgtc tcggcgacgg cggcgacgaa gaagacgaga aagccgagcg
      781 gctggagaaa aacgccccag cgcgggagga agccgagaat caacccgcct tgataggcca
      841 cgatctccga gagccgcagc gagggataga ccatgatcag cccgaccagg gacagcccga
      901 ggccgacctc gtaggagacg agctgggccg cgccccgcag cccgccgatc aaaccccagc
      961 ggttgcgcga cgaccagccg gaaaggagga tgccgtaaac tcccagcgag agcatcgcca
     1021 gaaggaacaa gagcccggcc gtgccgtcgt aaacctggag aaggacgggc gtcccgccga
     1081 tccggaccgc cggaccgaac ggcaccacgg cgacggccgc cgcgacgaag aaaaaggaaa
     1141 cgaacggggc cagggtatgg agaaccttgt cggcgaacgg cgggacgaag tcctctttga
     1201 aaatcatctt gatcgcgtcg gcgaacggct gaaaaaggcc gaacatcctc aggcccagga
     1261 cccgggcccg gttggccccg atccggtcct gcatgaccgc gctctgtttt cgctcgaccc
     1321 acgtcagaaa aagagcaagg aggagaagcc accccagggc gacggcgatc ttcaccagga
     1381 gaacagaaat ttcgctcatt cccttttccc tctccggacg gcctcgagaa gcgaccggag
     1441 gatgcccccc tccgtccgga tgccttccga aaccggccgg gcggccccaa attcccgccg
     1501 ccgtccttcg acgttcgtgt agcttccgga tttctcaaac gccgaggcgc agggaaggac
     1561 gaaatcgaag agatcgtccg cttccgtcag aaccggcgag acgaggactt tcgttcggat
     1621 cgaccgccac tcctccgggt ggaggatcca gggggtgacc tctccgcgga cgccggcgaa
     1681 aacgaacagg agatcggcgc gggctcggag ctcgtccaaa tccaggggac gcagatcggc
     1741 tcccccggcc gccaaggcgc cgcggcggtt gggcgtccgt tccggcgtcc ggaggaatcc
     1801 atcgcctccg tcgtcggggg gatcggccag ccggaggagc ggccggggat ggaatccgtc
     1861 ccggaacatc cgcacatcat cccattcttc gttggtcaat ccggaatgaa cgacgagcgc
     1921 caggcggccg gtccgttcgg tctcgtccag ggcccggatc ttggcgccca ggagggcgaa
     1981 cgccttctcg gcgctgagca cggcttcccc gccgccgccc ttattccaga cgatcgcccg
     2041 ggcccggcgc gattcgattt cgatccaagc gtaccggccg acgtcgcaga tccacggtcc
     2101 ggccggggtg gcgtcgggcc ggggacgaac gcggaagatg ccgcctgttt ccggatgccg
     2161 ggcgaagccg gggtgaacat cgacctcgat cccgcagccg cggccgcaga acgggcaaat
     2221 cgacggcttc ggctccaaaa accagggccg cgtccggaaa cgaaaggcgg cgtcggtgat
     2281 cgctccgacc gggcagagat cgacgaggtt gccggcgtaa ggcgtggcca ccgccgcccc
     2341 ctcgatgaag ccgatctccg acccgccgcc ccggccgaaa acacccagct cgcccgtctt
     2401 ggcgacgtcc ctcagaaacc ggacgcaccg ggtgcaaaga acgcagcgct ccctgtccag
     2461 gatcagccgc tctccgattt ttaaaagctt ctcccgccgc gtcttcggct cgcgcaggtc
     2521 gctcggaaaa agcccgtaat cccggtaata atcctggagc cggcactcgc ccgccttgtc
     2581 gcagatggga caatcgacct gatgatcggc caggaggagc tcgaggacgt tccgccgggc
//
LOCUS       DAOOXH010000189         2612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_48920_length_2612_cov_5.670708, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000189 DAOOXH010000000
VERSION     DAOOXH010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48920_length_2612_cov_5.670708"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PKV64_11910"
     CDS             <1..348
                     /locus_tag="PKV64_11910"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HPN17822.1"
                     /translation="LISGASLNGRFIEDSDRNGAMDPADKGLADILVILTPIDGAAQK
                     DLILNTYTDAGGVYRFVNILPGEYELSVDPETLPAGAVTAVPLPFKIKLEPGRTAGGV
                     DFLVIPRPVIRKK"
     gene            complement(349..1542)
                     /locus_tag="PKV64_11915"
     CDS             complement(349..1542)
                     /locus_tag="PKV64_11915"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPN17823.1"
                     /translation="MKKAASAAIILIFLGAACARRSLPGGTVRDAGFDPDRLARASTV
                     IGEAIARKELPGAVLLVARKGKIVLHEAYGDSRWIPDRRPMEKDMIFDMASLTKPVAT
                     AASVMILVESGRLSLWDRVKTYIPSFVPYRDWFGKNGEDARIWHLLTHTSGLPPYAEP
                     EVAAKACGRPCPPSALAEYIAALPKDNPPGEKFVYSCLGYITLAEIVRRVSGLSIDRF
                     SAENIFEPLGLRRTFYNPDPKISAQIVPTQILDGKPLLGVVHDPLARLQEGISGNAGL
                     FSTAKDLAVFGQMLLNKGEFRGRRIFSPAAVERMTSIYPRTEFSGRGLGWDLGSDYAS
                     NGGDLFGPRSFGHTGYTGTSLWIDPDTKTIVVLLTNSVHPEDKGTIVPLRARVANIVA
                     GAIVD"
     gene            complement(1539..>2612)
                     /locus_tag="PKV64_11920"
     CDS             complement(1539..>2612)
                     /locus_tag="PKV64_11920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HPN17824.1"
                     /translation="WVADMDFAAPPAVCEAVARRAAHGAYGYPSDPASVREAAAGWLR
                     TRFGWDVHPEEFSYAPGIVPALHACVRAFTKPGDGVIIQTPVYPPFFSAVEASGRWLV
                     RNPLRLENSRWTMDYDDLRRRVDDRVKMLFLCSPHNPVGRVWTEEELRKLAEICLERN
                     LVVVSDEIHGDLVFRGFRHIPFATLGPDIAARTITLIAPSKTFNVAGLAASLAVAGDR
                     GLREGFRKEFRDFGWELPNVFGLAAMEAAYRGGGSWLEALLVYLEEGADLLRNLFDER
                     LGPLRFFKPEGTYLGLVDGRGLGLAPERWKSFLLREAGVYLNDGAMFGPETEGFARLN
                     FACPHSVLREAAGRIGRALGGIR"
BASE COUNT          485 a          874 c          860 g          393 t
ORIGIN      
        1 ctgatctcgg gggcttcgct caacggccgg ttcatcgagg attccgaccg gaacggggcc
       61 atggaccccg cggacaaggg actggccgac atcctggtca tcctgacccc catcgacgga
      121 gcggcccaga aggacttgat cctcaacacc tacaccgacg ccggcggcgt ctaccgattc
      181 gtcaacatcc ttcccgggga atacgagctc tcggtcgatc cggagacttt gccggccggg
      241 gcggtcacgg ccgtccccct tcccttcaag atcaagctcg agcccggccg gacggccggc
      301 ggagtcgact tcctggtgat cccccggccg gtcatccgga aaaaataatc agtccacgat
      361 cgccccggcg acaatgttgg ccacccgggc gcgaagcgga acaatcgtcc ccttgtcctc
      421 gggatggacg ctgttggtca gcaggacgac gatggtcttg gtgtcgggat cgatccagag
      481 cgacgtgccg gtgtagcccg tatgcccgaa cgaccgcggg ccgaacagat cgcctccgtt
      541 ggaggcgtaa tccgagccga ggtcccagcc cagcccgcgg ccggagaact ccgtccgggg
      601 atagatcgac gtcatgcgct ccacggccgc cgggctgaaa atccgccggc cgcggaactc
      661 gcctttgttg agaagcatct ggccgaacac cgctaaatcc ttggccgtgg aaaacaagcc
      721 ggcgttccct gagatcccct cctgcagccg ggccagggga tcgtggacga cgccgagaag
      781 cggcttgccg tccaggatct gggtgggaac gatttgggcg gaaattttcg gatcgggatt
      841 gtaaaacgtc cggcgcaggc ccaagggctc gaagatgttc tcggcggaga aacggtcgat
      901 cgacaggccg gacacccggc ggacgatctc ggccaaggtg atgtatccca ggcagctgta
      961 gacgaatttt tcgcccggcg gattgtcctt gggcagggcg gcgatatatt cggccagggc
     1021 cgagggcgga cagggccgtc cgcacgcctt ggccgcgact tcgggctcgg cgtagggggg
     1081 gaggccggag gtatgagtca ggagatgcca gatccgggcg tcctccccgt ttttcccgaa
     1141 ccaatcccgg tagggaacga aggaagggat gtacgttttg acccggtccc agaggctgag
     1201 ccggccgctt tcgacgagga tcatcaccga ggccgccgtg gccacgggct tggtgagcga
     1261 ggccatgtcg aaaatcatgt ccttctccat cggccggcgg tccgggatcc agcggctgtc
     1321 gccgtaagcc tcatgaagga cgatttttcc cttgcgggcc accaacagga ccgcccccgg
     1381 caattccttc cgggcgatcg cctccccgat gacggtcgac gcccgggcca accggtccgg
     1441 atcgaagccg gcgtcccgga cggtgccgcc gggcagggac cggcgggcgc aggccgcccc
     1501 gagaaaaatc aagatgatcg ccgccgacgc cgcttttttc atcggatgcc tcccagggcc
     1561 cgcccgatcc tcccggccgc ctccctcagg acggaatgcg gacaggcgaa gttgagccgg
     1621 gcgaaaccct ccgtctccgg gccgaacatc gccccgtcgt tcaagtatac tcccgcctcg
     1681 cgcagaagga aggacttcca tcgctccggg gccaagccga ggccgcgccc gtccaccagg
     1741 cccaggtagg tcccctcggg cttgaaaaac cgaagaggcc cgaggcgctc gtcgaagaga
     1801 ttccggagca ggtcggcccc ctcctccagg tagacgagaa gggcttcgag ccaggagccg
     1861 ccgccccggt aagcggcctc catggcggcc aatccgaaca cgttgggaag ctcccagccg
     1921 aaatccctga attccttccg gaagccctcg cggaggcccc ggtccccggc gaccgccagg
     1981 gaggcggcca agccggcgac gttgaacgtc ttgctcggag cgataagagt gatcgtccgg
     2041 gcggcgatgt ccgggccgag ggtcgcgaag ggaatatgcc ggaaaccgcg aaagacgaga
     2101 tccccgtgga tctcgtcgga cacaacgacc aggttccgct ccagacaaat ctccgccagc
     2161 ttccgcagct cctcctccgt ccacacccgg ccgaccgggt tgtgggggct gcagaggaaa
     2221 agcattttga cccggtcgtc gacgcgccgg cggagatcgt cgtaatccat cgtccagcgg
     2281 ctgttttcaa ggcggagggg attgcgcacc agccagcgcc ccgacgcctc gaccgcggaa
     2341 aaaaacggcg gatagaccgg cgtctggatg atcaccccgt cgccgggctt ggtgaacgcc
     2401 cggacgcagg cgtgaagggc cgggacgatg cccggcgcat aggagaattc ctccggatgg
     2461 acatcccagc cgaagcgcgt ccgcagccag ccggccgccg cttcgcggac ggacgccggg
     2521 tcggacggat agccgtacgc cccatgggcc gcccgccggg cgacggcttc acaaacggcc
     2581 ggcggggcgg cgaaatccat gtcggccacc ca
//
LOCUS       DAOOXH010000190         2605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_49112_length_2605_cov_7.823922, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000190 DAOOXH010000000
VERSION     DAOOXH010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49112_length_2605_cov_7.823922"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PKV64_11925"
     CDS             <1..838
                     /locus_tag="PKV64_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPN17825.1"
                     /translation="DDIGLPFDEYDQGTFSAALEDQVSLGDKWQVVGGFSFDLIDKYS
                     GGTYSRVNPMIGLKFTPAETLDLHLSLARKSRFPNMRSMYSPSSGNPDLLSETGTNVE
                     LGFTWDPGVRLSGAAFMYEFKNMIDSITLSDGKKRYWNVGKAHINGFELQASKSLGWL
                     EGTLNYTYIDAANDTDDRPLDAMPASNLNFDLTVFPAGGLRISLFGLAASKSDWYEFS
                     AKKNYEIPAYFNLDAVLAYDFGRFEIFVKATNLFDDYIYTEPIFPWRARFFEFGVRVD
                     VF"
     gene            1529..>2605
                     /locus_tag="PKV64_11930"
     CDS             1529..>2605
                     /locus_tag="PKV64_11930"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPN17826.1"
                     /translation="MCDTFVARGEAAAGGVTIFGKNSDREPNEAQALEFHPRRRGEEG
                     EEVRLTYLAIPQVRETRAVLISRPFWMWGAEIGVNEDGLAIGNEAVWTKMPLSLKPGL
                     LGMDMLRLALERASTAGQALEVIAGLLHDYGQGGICGFRDRKMTYHNSFIIADPEEAW
                     VLETAGPLWAARKVERAASISNGLTLGEEIDRMHPELIATARAKGWLKKGETFHFSRC
                     YSDWFYTKFSACRRRRVRSLALVEGRRGSMDAAAAMAVLRDHDGDDYRPDSHFLGNRV
                     CAHAANGLTRKATQTTAALAACLAREPERRAVWATGTAAPCTSLFKPVWLGDGMLPDL
                     GPGLSGMFDAGTLWWRHERFHRTVL"
BASE COUNT          490 a          772 c          823 g          520 t
ORIGIN      
        1 ggacgatatc ggtctcccgt tcgacgagta cgaccagggg acgttttccg cggccctgga
       61 agaccaggtc tcgctcggcg acaagtggca ggtggtcggc ggcttcagct tcgacctcat
      121 cgacaagtac tccggcggaa cgtattcccg ggtcaatccg atgatcggcc tgaaattcac
      181 cccggccgaa acgctggacc tgcacctctc gcttgcgcgg aagtcccgct tccccaacat
      241 gcggtcgatg tactcgccct ccagcgggaa cccggacctt ctgagcgaga cggggacgaa
      301 cgtggagctc ggattcacct gggatccggg cgtccgcctg tcgggcgcgg ccttcatgta
      361 cgagttcaag aacatgatcg attcgatcac cttgtccgac gggaagaagc gctactggaa
      421 tgtcggcaag gcccacatca acgggtttga gctgcaggcg tcgaagtcgc tcggctggct
      481 cgaggggacg ctgaactaca cctatatcga cgccgccaac gacacggacg accggccgct
      541 ggacgcgatg ccggccagca acttgaactt cgacctcacg gtgttcccgg ccggcggcct
      601 gcggatcagc ctcttcggcc tggctgcttc gaaatccgat tggtacgaat tctcggcgaa
      661 gaagaattac gagatccccg cgtatttcaa cctcgacgcg gtcctggcgt acgacttcgg
      721 ccggttcgag atattcgtca aggcaaccaa ccttttcgac gattacatct acaccgaacc
      781 cattttcccc tggcgggccc ggttcttcga attcggggtc cgggtggatg tcttctgacc
      841 tccccggtca gtgatggggg aacgcccggg gcgcggttgc gcctcgggcg tttttaattc
      901 ggaacctgtt aattcggaac ctggttatcc atttccgtat tcataaaacg tccggttctc
      961 aaagatcgat cgcgttctga tcatcaaaaa tcgtctcgag gccgtcgccg cgaggaattg
     1021 agccgttcgt ctccgcggaa agaggaaaaa accggaacgg ctttgcctcg atgttcatcg
     1081 agagggtttt gttctccttc ctcttttgac tcaccgtgtc cgcacgtgtt ttttcagccg
     1141 tggtccggcg ttgagaattt ccaggactgc gaaatacgga aatggttaaa caggctccga
     1201 tttaccgctg aaagaggaaa aaacggctga accggccgtg aaatatagtt tttcccgcca
     1261 tgcggctttc tcccgctgtt tgtgaggcgg ccgttccttg atgttcaagc gcacgggctg
     1321 tttccctttc cttgttcact cccggcgccc gcgtgcgttt ctccgccgcg gccggccggc
     1381 gtggatgtgc ggggcaggga aatacggaaa tggttaaaca ggctccgatt tcgaaacggg
     1441 tcccggtcgg ccgccgaaat agagttcatc ggtattgcgt ctccccgttt ctcggataaa
     1501 atagaagcga gaaaggagaa aacggaaaat gtgcgatacg ttcgtggccc ggggagaggc
     1561 ggcggcggga ggagtgacga tctttggaaa aaacagcgac cgcgagccga acgaagccca
     1621 ggcgctggaa ttccatcccc gccgacgagg agaggaaggg gaggaagtcc ggctgacgta
     1681 tctggcgatc ccccaggtcc gggagacgcg ggccgtcctg atctcacgcc cgttttggat
     1741 gtggggggcg gaaatcggag tcaatgaaga cggtttggcc atcggcaacg aggcggtctg
     1801 gacgaagatg ccgctttccc tcaagcccgg cctcctcggc atggacatgc ttcgcctggc
     1861 cctggagcgg gcctcgacgg ccgggcaggc tcttgaggtc atcgccggcc ttctccacga
     1921 ttacggccaa ggcgggatct gcggcttccg cgaccggaag atgacctatc acaacagctt
     1981 tatcatcgcc gatccagagg aggcctgggt gctggagacg gccggtccgc tgtgggcggc
     2041 gcgcaaggtc gaaagggcgg cgtccatcag caacggcctg acgctcggcg aggagatcga
     2101 ccggatgcat cccgaactca tcgccacggc ccgggccaag ggctggttga agaaggggga
     2161 gacgttccat ttctcccgtt gctattccga ctggttctat accaaattct ccgcttgccg
     2221 tcggcgccgg gtgaggtcct tggccttggt cgagggacga cgcggctcga tggacgcggc
     2281 cgcggccatg gccgttttac gcgatcatga cggagatgat taccggccgg acagccattt
     2341 cctcggcaac cgcgtctgcg cccacgcggc caacggattg acccgcaagg cgacccagac
     2401 gacggccgcc ctggccgctt gcctggcgag ggaaccggag cggcgggcgg tgtgggcgac
     2461 gggaacggcc gctccttgca cgtcgctttt caaaccggtt tggctcggcg acgggatgct
     2521 gccggatttg ggccccgggt tgtccggaat gttcgacgcg ggaacgctct ggtggcgcca
     2581 cgaacgcttc caccggacgg tgctc
//
LOCUS       DAOOXH010000191         2478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_52832_length_2478_cov_6.033017, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000191 DAOOXH010000000
VERSION     DAOOXH010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52832_length_2478_cov_6.033017"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PKV64_11935"
     CDS             complement(<1..189)
                     /locus_tag="PKV64_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17827.1"
                     /translation="METYRRHSLDKKMLYATIILMVIGLVMIMSASGVYAEQAYQKKL
                     YFFLQQLGGFAVILVLLAI"
     gene            complement(195..1559)
                     /gene="murD"
                     /locus_tag="PKV64_11940"
     CDS             complement(195..1559)
                     /gene="murD"
                     /locus_tag="PKV64_11940"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPN17828.1"
                     /translation="MTMNLDGRNVLVVGLARTGEATAAFLLRRGARVTVTESKSRAEL
                     GSKAETWVRRGVTLEAGGHTPAAFLEADLIIPSPGVPRLPEIAAARDRGIPVWSEIEL
                     ASRFLKGSIVGLTGTNGKSTTTTLLHRILKDAGRPAFLAGNIGTPLISFVDRSRDDHL
                     YVTEISSFQLEYIHAFRARLALVLNLSGNHLDWHGTMDDYAAAKAKLVLGQKAGDAAV
                     LNREDARVWAWKEAAVSDVFAFSGKRPVRRGAFVRDGWVVVRDGGDERRILPVSEIKL
                     PGLHNRENVLAAAAAARLLGIAPSRIRASVRGFRGLEHRLEPVATVGGVSFINDSKAT
                     TVDATLKALAGFTRPVVLILGGKDKGSDFRPLRKAVRRGVKAVVLVGSAREKIREALA
                     GTVPLLEAETYPEVVRTARAAAAPGDIVLLAPACTSWDMFRDFEERGRVFKREVRRLA
                     RRKG"
     gene            complement(1556..>2478)
                     /gene="mraY"
                     /locus_tag="PKV64_11945"
     CDS             complement(1556..>2478)
                     /gene="mraY"
                     /locus_tag="PKV64_11945"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPN17829.1"
                     /translation="VRSDGPASHLSKQGTPTMGGLLIITATIVPVLLWGNLANTYVLL
                     TMGTMIAFGGIGFLDDYMKVRKKQSRGLFARSKLLFQILLAAVFALFVMLLSANGDFN
                     LHLFFPILKRWAPYLGWLYAPWIIFIVVSSTNAVNLADGLDGLAIGLTLIGAAALTAL
                     TYIAGHAVFSNYLYIARVPLAAELTVFGGALVGACLGFLWFNSHPAQVYMGDVGALSL
                     GATLAAVAILIKQEFLIFAVGGVFILEAVSVLLQVSYFKATKGKRLFKMAPLHHHFEL
                     LGWPEEKVVVRFWILGLIFAFFSLATLKLR"
BASE COUNT          455 a          767 c          877 g          379 t
ORIGIN      
        1 gatcgcgagc aggaccagga tgacggcgaa tccgccgagc tgctggagga agaagtagag
       61 cttcttctgg taggcctgct cggcgtaaac ccctgaggcg ctcatgatca tgaccaaccc
      121 gatgaccatg aggatgatcg tcgcgtacag catcttcttg tccaaggagt ggcggcggta
      181 ggtttccatc gccgttatcc cttcctccgg gccaggcggc ggacttcccg cttgaacacc
      241 cggccgcgct cctcgaaatc ccgaaacatg tcccagctcg tgcaggccgg ggcgagcagg
      301 acgatgtcgc cgggagcggc cgcggcccgg gccgtccgga cgacttccgg gtatgtctcg
      361 gcctcgagca ggggaacggt cccggcgagg gcttcccgga ttttttcccg ggcggagccg
      421 acgaggacca ccgctttgac gccccgccgg acggccttcc ggagcggccg gaaatcggat
      481 cccttgtcct tgccgccgag gatgagaacg accggccggg tgaagccggc cagggccttg
      541 agggtcgcgt ccacggtcgt ggccttggag tcgttgatga aggaaacgcc gccgacggtg
      601 gcgaccggct cgagacggtg ctcgaggccg cggaagccgc ggacggaggc cctgatccgg
      661 gacggggcga tcccgagaag gcgggcggcg gccgccgcgg ccaggacgtt ctcccggttg
      721 tggaggccgg ggagcttgat ctcggacacg ggaaggatcc gccgctcgtc gccgccgtcg
      781 cggacgacca cccagccgtc gcggacgaac gcgccccggc ggaccggccg cttgccgctg
      841 aaggcgaaga cgtcggagac cgccgcctcc ttccaggccc agacccgggc gtcctcgcgg
      901 ttgaggacgg ccgcgtcccc ggctttctgg ccgaggacga gcttggcctt ggccgcggcg
      961 taatcgtcca tcgttccgtg ccaatccaga tgattgccgg acagattcaa aaccagggcc
     1021 agccgcgccc ggaaggcgtg gatgtattcc agctggaagc tcgagatttc ggtgacgtag
     1081 agatgatcgt cgcggctccg gtcgacgaag gagatcaggg gcgtgccgat gttgccggcc
     1141 aggaaggccg ggcggccggc gtccttcagg atgcggtgaa ggagggtcgt cgtcgtcgat
     1201 tttccgttcg tcccggtgag gccgacgatc gagcctttca gaaaacggga cgccagctcg
     1261 atctccgacc agacggggat tccgcggtcc cgggcggccg cgatctcggg aagccggggc
     1321 acgcccgggc tggggatgat caaatcggcc tcgagaaagg cggccggcgt gtggccgccg
     1381 gcttcgagcg tcactccgcg ccggacccag gtctcggcct tggaaccgag ctccgcccgg
     1441 gacttgctct ccgtgacggt cacccgggct ccgcggcgga gaaggaacgc ggccgtcgcc
     1501 tcgcccgtcc gggccaggcc gaccaccagc acgttccgtc cgtccaaatt catggtcatc
     1561 tcaatttcaa cgtggccagg ctgaagaaag cgaagatgag gccgaggatc cagaaccgga
     1621 cgacgacctt ctcctccggc cagcctaaaa gctcgaagtg atgatgaagg ggggccatct
     1681 tgaagaggcg tttccccttg gtcgctttga agtaggacac ttgcagaagg accgagacgg
     1741 cttcgaggat gaaaacgccc cccacggcga agatcaagaa ttcctgcttg atgaggatgg
     1801 cgacggcggc cagcgtcgct cccagggaaa gggcgcccac atcgcccatg tacacctggg
     1861 ccggatggct gttgaaccag aggaagccga ggcaggcccc gacgagcgcg ccgccgaaga
     1921 ccgtgagctc cgcggccagg gggacgcggg cgatgtacag gtaattggaa aacacggcgt
     1981 ggccggcgat atacgtcagg gcggtcaggg ccgccgcccc gatcagggtc aggccgatgg
     2041 ccaggccgtc cagcccgtcg gccaggttga cggcgttggt cgaggacacg acgatgaaga
     2101 tgatccaggg ggcatagagc cagccgagat agggcgccca tctcttgaga atggggaaga
     2161 aaagatggag gttgaaatcc ccgttggcgc tcaggagcat gacgaacagg gcgaaaacgg
     2221 ccgccaggag gatctggaac agcagcttgc tccgggcgaa gaggccgcgg gactgctttt
     2281 tccggacctt catgtagtcg tcgaggaacc cgatcccgcc gaaggcgatc atcgtcccca
     2341 ttgtcaacag gacgtacgtg ttggccaggt tgccccagag aaggacgggg acgatcgtgg
     2401 ccgtgatgat caggaggccg cccatcgtcg gcgtgccctg cttgctcagg tgggaggccg
     2461 gtccgtccga gcggacga
//
LOCUS       DAOOXH010000192         2426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_54489_length_2426_cov_4.713623, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000192 DAOOXH010000000
VERSION     DAOOXH010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54489_length_2426_cov_4.713623"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PKV64_11950"
     CDS             complement(<1..427)
                     /locus_tag="PKV64_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/galactose cotransporter"
                     /protein_id="HPN17830.1"
                     /translation="MQNPGSLMSLNGVDMAVVALYFVVVLAIGFYLKRFTKTGNDFFM
                     AGREMTSWVAGLSFVAANLGALELMGWAASSYQYGILAAHWYWIGAIPAMLFLAIVMM
                     PFYYISKTHSVPGYLQLRYGNSSRSLSALCFGVMTVLMSG"
     gene            complement(916..1383)
                     /locus_tag="PKV64_11955"
     CDS             complement(916..1383)
                     /locus_tag="PKV64_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HPN17831.1"
                     /translation="MEDKIKKVSIIISKGGLEGIYPGLIMANGARMEGIETNLFFTFF
                     GMDAIIKKRMGRIKVATVGNPGMHMPSLVGILPGMSAMATSMMKKTMEKLDIPPVPEY
                     IEMIADAGGTLYACKATVDMFKLKKEDFCPQVNSIINVGRFYEISAGGHIIFT"
     gene            complement(1412..1729)
                     /locus_tag="PKV64_11960"
     CDS             complement(1412..1729)
                     /locus_tag="PKV64_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015655054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HPN17832.1"
                     /translation="MPLKTYGNTTVDVDAEGYLTDFSQWTRTVAEAIAAEEGFAALGP
                     AHWKVIEFMQKEYKENGQVPSIRRLNKSGVASTKELYELFPGGPAKKAARIAGLKKPE
                     GCV"
     gene            complement(1761..>2426)
                     /locus_tag="PKV64_11965"
     CDS             complement(1761..>2426)
                     /locus_tag="PKV64_11965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HPN17833.1"
                     /translation="FTKPVAAAALGALLEKKRIRMVADFNAERIDASARKIIGYDGRE
                     VPYDLLVTVPTNMGAEMIERSGMGDEFRFLPTDPRTLRSKKHENIFALGDATDVPASK
                     AGSVVHFQSEILTENILRAIDGRPLEPAFDGHANCFIEMGRGKAILIDFNYETEPLPG
                     RFPFRVFGPLPLLKPSRLNHWGKLAFRGIYWRLLLRGRRIPFVPSRMKMTGKKRPAAA
                     PGA"
BASE COUNT          461 a          736 c          756 g          473 t
ORIGIN      
        1 tgccgctcat caggacggtc atgaccccga agcacaaggc cgaaaggctc cggcttgagt
       61 ttccgtagcg caactgaagg tatccgggga cggaatgcgt cttggagatg taatagaacg
      121 gcatcatgac gatggccaga aagagcatcg ccgggatcgc cccgatccag taccagtggg
      181 cggccaggat gccgtattga taactcgacg cggcccagcc catgagctcc agggctccga
      241 ggttggccgc gacgaagctc aagccggcga cccaggaggt catctcccgg ccggccatga
      301 aaaagtcatt gcccgtcttg gtgaagcgct tcagatagaa gccgatcgcc agaacgacga
      361 cgaaatagag ggcgacgacg gccatatcga cgccgttgag cgacatcagc gagccgggat
      421 tctgcatcgt gtcctccttg tcatggaaaa ccgggagcat ccggaaaggg acgcagggag
      481 ggaaacatat cgaccggaat cttattccgg ccggaggcgg ctgtcaatcc ggcgaaggcg
      541 tttcgatgag gggattccgg gccgatccct ccggagaagt cttccttcca agtcttgggg
      601 agcgaagaac ttgcccggcc ctatggttcc tcccgcggaa ggcgccttcg tttttcacga
      661 cggctctcga tttcgcccaa gcgaatcggc ctttcttttt tcccggggag gtctttggct
      721 gcgttccccc gggccggatt cgggccggaa cgcgaacgcc cccttttccc ctcccgcctt
      781 ccgggacggg gaggaagacg ctcgggccga gggcctctcc tcagggaaac ccgaacagcc
      841 ggcggaaagc ggacgaaacg cgcggcgttc cccgggccgg cggatccggc cggttcgtac
      901 agcgcgtccg gcgcgtcagg taaaaatgat gtgtccgccg gccgagatct cgtagaaccg
      961 gccgacgttg atgatcgaat tcacttgagg gcagaaatcc tctttcttga gcttgaacat
     1021 gtcgaccgtg gccttgcagg catagagcgt gccgccggcg tcggcgatca tctcgatata
     1081 ttcgggaacg ggaggaatgt cgagcttctc catcgtcttc ttcatcatgg aggtggccat
     1141 ggccgacatg cccggcagaa tgccgacaag gctgggcatg tgcatcccgg gatttcctac
     1201 cgtggcgact ttgatccggc ccattctctt cttgatgatc gcgtccatgc cgaaaaaagt
     1261 gaagaataaa ttggtttcga tgccctccat ccgggctcca ttggccatga tcagccccgg
     1321 gtagattccc tccagccctc ccttggagat aatgatcgag accttcttga ttttgtcttc
     1381 catggctcct cctttcgaaa cggccggaga atcagacgca gccctcgggc tttttcaagc
     1441 cggcgatccg ggccgccttc ttggccgggc cgcccggaaa cagctcgtaa agctccttgg
     1501 tggaggcgac ccccgacttg ttgagccggc ggatgctcgg gacctggccg ttctccttgt
     1561 attccttctg catgaactcg atgaccttcc agtgagccgg cccgagggcg gcgaagccct
     1621 cttccgcggc gatcgcctcg gcgacggtcc gcgtccactg ggaaaaatca gtcaggtaac
     1681 cctcggcgtc gacgtcgacc gtggtgttcc cataggtttt gagaggcatg gcgtatctcc
     1741 ttctttcatc ggcgatgcga tcaagcgccc ggagcggccg ccgggcgctt tttcccggtc
     1801 atcttcatcc gggaagggac gaacgggatc ctcctccccc ggagaagcag gcgccaatag
     1861 atcccgcgga aggccagctt cccccagtga ttgagccggc tgggcttgag cagggggagc
     1921 ggcccgaaca cccggaaagg aaaccggccg ggcagcggct ccgtctcgta gttgaaatcg
     1981 atcaagatag ccttgccccg gcccatttcg atgaagcaat tggcatggcc gtcgaacgcc
     2041 ggctcgagag ggcggccgtc gatcgcccgg agaatattct ctgtcaggat ctccgactgg
     2101 aagtgaacga cggagccggc cttggaggcg gggacgtccg tggcgtcgcc gagggcgaag
     2161 atattttcat gttttttcga gcgcagcgtg cggggatcgg tggggaggaa gcggaattcg
     2221 tcgcccatcc ccgagcgttc gatcatctcc gcgcccatgt tcgtcggaac ggtgacgaga
     2281 aggtcatagg gcacctcccg gccgtcatac ccgatgattt tccgggccga cgcgtcgatg
     2341 cgctcggcat tgaaatccgc gaccatccgg atgcgcttct tctccaggag agcccccagg
     2401 gccgcggccg ccaccggctt ggtgaa
//
LOCUS       DAOOXH010000193         2343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_57305_length_2343_cov_2.994755, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000193 DAOOXH010000000
VERSION     DAOOXH010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57305_length_2343_cov_2.994755"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PKV64_11970"
     CDS             complement(<1..556)
                     /locus_tag="PKV64_11970"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPN17834.1"
                     /translation="MKKQNAAAPPRRWPWFAGAIAAAAAAILAFHLLTGRGSFPRIAR
                     EWSSLGVDKPNVVIVTLDTTRADRLGAYGCRAARTPVLDALAGRGALFEQCATVAPLT
                     LPAHSTIFTGLPPTFHGVRVNGNTALSDEQTTLAEVMASRGYACGAFIAAFVLDGRWG
                     LKQGFDHYDDRFDLKKYKQLDLGAV"
     gene            complement(565..>2343)
                     /locus_tag="PKV64_11975"
     CDS             complement(565..>2343)
                     /locus_tag="PKV64_11975"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HPN17835.1"
                     /translation="FGRKDADPRTGNALTGTMCVAAESLPGRAEVSVLADFDPLSDGA
                     GVWTSFAGTGILPGGETSRTTSGGEALGAALAVRLTLRPGEKVEFPIVLAWDFPYAEF
                     EPGARHLRKYTAFFGSDGNQAAAIAREALDKWREWERAVDDWQRPIIGDRSLPDWFKQ
                     ALINELYILPETSLWDAATNLHTYLESADYLMYGTFDVDSYCWHVPKLWPGLERENLR
                     FFSRAVEMEDASFKAYQYASAFPGEVPPDKLSYYWSINKAAGMVPHDLGSPRKRPWVI
                     LNAFDWQNGNVWKDLNPKFPLRALRALRDGGPGDEDLLKRSFRASVSALDTLEKKFGD
                     PQSHLPRNEGIPDQTYDTWRMKGETAYVGMLWLASLQAAAALGEDCRRAGMESLDGLP
                     VGEIVARYRSWFEAGRTALQTLWNESSGCFNIDAETDDIMTDQLFGVWYAAMVGLEND
                     EAGRIVPPSQVRRALRTVFEKNVLGFGNGRLGAVNGRTAAGTQLFSQQGDEVWVGTAY
                     AFASNLILHGLRDEGLQTAWGVYHVVYSPYGQGYFFKTPEAYLNPAELVWNDRSRTYG
                     DRLFRAMKYMRPGAVWAVYEALLKTR"
BASE COUNT          399 a          754 c          790 g          400 t
ORIGIN      
        1 ggaccgctcc caggtcgagc tgcttgtact tcttgaggtc gaaccggtcg tcataatgat
       61 cgaatccctg tttgagcccc catcggccgt cgaggacaaa ggcggcgatg aacgctccgc
      121 aggcgtaccc gcgggaagcc atcacctcgg ccagtgtcgt ctgctcgtcg ctcagggcgg
      181 tgttaccgtt cacccgaacg ccgtgaaagg tcggcgggag gccggtgaag atggtggagt
      241 gggcgggaag ggtcaggggg gcgacggtgg cgcattgctc gaacagcgcg ccccggccgg
      301 ccagggcgtc cagaacgggc gtgcgggcgg cccggcagcc gtaggcgccg aggcgatcgg
      361 cccgggtggt atcgagggtc acgatcacga cattgggctt gtcgacgccg agggacgacc
      421 attcccgggc gatccgcggg aaggatccgc ggcccgtcag cagatggaag gccaggatcg
      481 ccgcggccgc ggcggcgatc gcgcccgcaa accacggcca gcggcggggc ggggcggcgg
      541 cgttttgttt tttcacggcg gctcttacct cgttttcagc agggcctcat aaacggccca
      601 gacggccccg ggcctcatgt atttcatggc ccggaacagg cggtcgccgt aagtccggga
      661 ccggtcgttc cagacaagct cggccggatt caaatacgcc tcgggcgtct tgaaaaagta
      721 tccctggccg taggggctgt agacgacgtg atagacgccc caagcggttt ggaggccctc
      781 gtcccgcaac ccgtggagaa tcaggttcga ggcgaaggca taagcggtcc ccacccaaac
      841 ctcgtcgccc tgctggctga aaagctgggt cccggcggcc gtccgtccgt tgaccgcgcc
      901 gagccggccg tttccgaacc cgagaacgtt cttttcaaag acggtcctca gagcccgccg
      961 gacttgagac gggggaacga tccgtccggc ctcgtcgttt tccagcccga ccatggccgc
     1021 gtaccagacg ccgaacagct gatcggtcat gatgtcgtcg gtctcggcgt cgatgttgaa
     1081 gcagccgctc gattcgttcc agagcgtctg cagggcggtc cggcccgcct cgaaccagga
     1141 gcggtaccgg gcgacgatct ccccgaccgg caggccgtcc agcgattcca tccccgcccg
     1201 ccggcagtct tcccccaagg cggcggccgc ttggagcgag gccagccaga gcatcccgac
     1261 ataggccgtc tcccctttca tccgccacgt gtcgtacgtc tgatcgggga tgccttcgtt
     1321 tcgagggaga tggctttggg gatcgccgaa cttcttctcc agggtgtcga gggcggagac
     1381 cgacgcccgg aacgatcttt ttagaagatc ctcatccccc ggcccgccgt cccggagcgc
     1441 ccgcagggcc cggaggggaa atttgggatt gaggtccttc caaacgttgc cgttctgcca
     1501 gtcgaaggcg ttgagaatga cccagggccg tttccgcggc gacccgagat catggggaac
     1561 catgcccgcg gccttgttga tgctccagta ataggagagc ttgtcgggcg gcacctcgcc
     1621 gggaaaagcg ctcgcgtact ggtacgcctt gaaggaggcg tcctccatct ccacggcccg
     1681 gctgaaaaag cgcaggtttt cccgctcgag cccgggccag agcttaggaa cgtgccagca
     1741 gtaggagtcg acgtcgaacg ttccgtacat gagatagtcg gcgctctcga gatacgtgtg
     1801 gagattggtc gccgcgtccc acaggctcgt ttcgggaagg atgtagagct cgttgatcag
     1861 ggcctgcttg aaccagtccg gcaggctccg gtcgccgatg atcggccgct gccagtcgtc
     1921 gacggcccgt tcccattccc gccatttgtc cagggcttcc cgggcgatcg ccgccgcttg
     1981 atttccatcc gatccgaaaa aggccgtgta tttcctgagg tggcgcgccc cgggctcgaa
     2041 ctcggcgtat gggaagtccc aagcgaggac gatcgggaac tcgaccttct ctccgggccg
     2101 aagagtcaga cggacggcca gggcggcgcc caaggcctcg ccgccggacg tggtccgcga
     2161 cgtctccccg ccgggaagaa tcccggttcc ggcgaaggac gtccaaacgc cggccccgtc
     2221 ggagagggga tcgaaatccg ccaggacgga cacttcggcc cggcccggaa gggattcggc
     2281 ggcgacgcac atcgtcccgg tgagggcgtt tccggtccgg ggatcggcat ccttgcggcc
     2341 gaa
//
LOCUS       DAOOXH010000194         2292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_59142_length_2292_cov_6.844435, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000194 DAOOXH010000000
VERSION     DAOOXH010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59142_length_2292_cov_6.844435"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2292
                     /locus_tag="PKV64_11980"
     CDS             <1..>2292
                     /locus_tag="PKV64_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17836.1"
                     /translation="AAVRRKAFRPAWAAAILAAAGGFLALVLPPLVVSGSAERNLDIL
                     RKKARVIQRSYASLARTQEQALDRLTRTPFPRAAADQFALFKSIGLDPETEGVALYDS
                     ERRLRIWLGRAVNLEPLFSGSPPSLTPSFRPKQILVRDRASSVLTLIVRLETGEVLAM
                     HRLLAFVPGFPSPYLDEYGFLPVRVRRSAAIDYWDFREDLSSFERLFRGHQDEFISTP
                     RSAGETPSLLFPLRTAEGRIIATAGLRSPAPAARRLALRETLFFSALAALAAALLLFV
                     AAGFLSPAYFPKSRLRSAAVFLLALFGFRAVFIPLGRLGPAAASPLFSPGQAGFVSWG
                     NLTGSPFDIFLTALTLFGLAAGLTRLLGRRTDPGRKASPATAVAGLLSLPFLFAGVLL
                     IIERIAIHSNVHLLRFFPSGAFLLLHAAIFLLAAAAAVPAAAVLKRHFDRRPAPGWTV
                     AAYALLSASLLWILRPSAPGRALLFGSVLGLAPAAFSLARRRWKKTAAILALLAASIG
                     IFVTIRQASSAKSRSLAEGFLRQTIETRDSWARFLLDDSMRALGRSRRDVARFLHRPE
                     DSPDMARRLWGGTLVARFNWYSGLEIHDSEGTTLSRFALNVPNMFRLAEDLPLRPEGS
                     VVRLPLPFMGKTEEFLIGYRDWVEDGDLLGRTILYVSLNDALLPFLYSANPYFELLRS
                     NSLPSLVQFDFRFAVFSASGDILFNPSRLSTGLPSSLAGDPALDGPGVWTGFRDKDER
                     FDLYAFRRADRIQAVLIPRPTAVG"
BASE COUNT          276 a          860 c          659 g          497 t
ORIGIN      
        1 agcggcggtg agaagaaaag cgtttcggcc ggcctgggcc gccgcgatcc tggccgcggc
       61 cggaggtttc ctggctctgg tccttcctcc cctcgtcgtc tcgggaagcg ccgagcggaa
      121 cctggacatc ctcagaaaaa aggcccgggt catccagcgc tcgtatgcct cgctggcccg
      181 gacccaggaa caggccctgg accgcttgac ccggacgccg ttcccccggg cggccgccga
      241 ccaattcgcc ctcttcaagt cgatcggcct ggatccggaa accgagggcg tcgccctcta
      301 tgattcggag agacggctcc ggatctggct cggccgggcg gtgaatctcg aaccgctgtt
      361 ttcgggctct cccccctccc tgacgccgtc gttccggccg aagcaaattc tcgtccgcga
      421 ccgggcgtct tccgtgctca ctctgatcgt ccggctggag acgggcgagg tcctggccat
      481 gcaccgcctc ctggccttcg tccccgggtt tccatcgccc tacttggacg aatacggctt
      541 cctgcccgtc cgcgtccgcc gctccgcggc catcgactac tgggatttcc gcgaggatct
      601 ctcctccttc gagcgtttgt tccgcggcca ccaggacgaa ttcatcagca cgccccgctc
      661 ggcgggcgaa acgccctcgc tgctgtttcc cctccggacg gccgaggggc ggatcatcgc
      721 cacggccggt cttcgttcgc cggccccggc cgcccggcgt ctcgccctgc gcgaaacgct
      781 gttcttctcg gccctggccg ccctggccgc ggccttgctg ctttttgtcg ccgccggatt
      841 cctctccccg gcgtatttcc ccaagagccg cctccgatca gcggcggtct tccttcttgc
      901 ccttttcggc ttccgggccg tcttcatccc cctcggccgg ctcgggccgg ccgcggcctc
      961 tcctctcttt tccccgggcc aagccggatt cgtctcttgg ggaaacctga ccggatctcc
     1021 cttcgatatc ttcctgacgg ccctgacgct cttcggactg gccgccggcc tgacgcgctt
     1081 gctcggaagg cggacggacc caggccggaa agcgagcccg gcgacggccg tggcggggct
     1141 tctcagtctt cccttcctgt tcgccggcgt tcttttgatc atcgagcgga tcgcgatcca
     1201 ttccaacgtc catctcctcc ggtttttccc gagcggagcg tttcttctcc tccacgcggc
     1261 gatttttctc ctggcggcgg cggccgccgt cccggcggcg gccgtcctga aaagacattt
     1321 cgaccgacgg cccgcgccgg gctggaccgt cgcggcgtac gcccttctgt ccgcctcgct
     1381 tctttggatt ctccgccctt cggctccggg gcgggccctt ctcttcgggt ccgtcctggg
     1441 tttggccccg gccgctttct ctctcgcccg gcggcgttgg aaaaaaaccg cggcgatcct
     1501 cgctcttctc gccgcttcga tcggaatctt cgtcacgatc cgccaagcgt cctcggccaa
     1561 gtcgcgctcc ctggccgagg gattcctccg ccaaacgatc gaaacccgcg actcctgggc
     1621 ccgcttcctg ctggacgatt cgatgcgggc tttgggccgc tcgcgccggg acgttgcccg
     1681 ctttctccac cgccccgagg attcccccga catggcccgc cgtctctggg gcggcaccct
     1741 ggtcgcccgg ttcaattggt attcgggctt ggaaatccat gattccgagg ggacgaccct
     1801 ctcccggttc gctctcaatg ttcccaacat gttccgtctg gcggaggacc ttcccctccg
     1861 gccggaggga agcgtggtcc gtctcccctt gcccttcatg ggcaaaacgg aggaattcct
     1921 catcggctac cgcgattggg tcgaggacgg cgatcttctc gggcggacga tcctctatgt
     1981 ttcattgaac gacgccctcc tgccctttct ctattcggcc aatccctatt tcgagctgct
     2041 ccgctcgaac tccctgccgt cgctcgtcca attcgatttc cgtttcgccg tcttcagcgc
     2101 ctcgggggac attcttttca atcccagccg gctttcgacc ggccttcctt catccctggc
     2161 cggcgatccc gccctggacg gacccggagt atggaccgga ttccgcgaca aggacgaacg
     2221 cttcgatctc tatgccttcc gccgggcgga tcggattcag gccgttctga tcccccgtcc
     2281 gacggccgtc gg
//
LOCUS       DAOOXH010000195         2259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_60380_length_2259_cov_4.765880, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000195 DAOOXH010000000
VERSION     DAOOXH010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60380_length_2259_cov_4.765880"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="PKV64_11985"
     CDS             complement(<1..1208)
                     /locus_tag="PKV64_11985"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPN17837.1"
                     /translation="MDADSSAGLFDVAVIGGGVVGCAVLRELSRYRLRLVLLERRSDL
                     AEGVSKGNSGVIHAGFNVRPGSLKARMNAAGLRAIYGLASGLGVPHRKTGKLVVAFDE
                     DGRRHLEALRAQGERNGTPGLDIVGAAAIRNLEPEVRGAWALFSPWTGIISPYEFTIA
                     LAENAWRNGAEVRLDSGVTAADCRPDRFILETPAGPVSARWVVNCAGLQADEVAGLAG
                     PADFRIRPFKGEYLVLDKAAGDLLRMPVYPVPPQDESSLGIHLTPTTEGPVLLGPSGE
                     STDDKDEAGSTRGVMDRLKAEAFGLVPALGRVPVIHAYAGSRPKLDGLAAAGGPADFL
                     VAESRRHPRWINLGGIESPGLTAAPSLAEHVAGLIGAREDLRPREGFEPGLPGRPRFA
                     RLDDADRAARA"
     gene            1469..>2259
                     /locus_tag="PKV64_11990"
     CDS             1469..>2259
                     /locus_tag="PKV64_11990"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN17838.1"
                     /translation="MRKKILFSASVFHALTDASTVVIPTIFPILYNRGFLITRYSQIG
                     LLSNLGLIATVLFQFVVVRLSYRRDYRIFLLASGLGICACLALTPLAGSFFVLLLFFL
                     LLRLAASVYHPLIIAWISKSAADSDRELDRAMGIQSGSGNVGVMLAYLTVGFLAQRWT
                     WKTPLFAWAGLGLLLLIAVLLTLRGVPSRNEEIRPLREAQSWVRSLAAVKRFVPGFFF
                     GGMGWNVTVFFAPSLLNHRFGVPMGQTGLFLALWMGIGTITGYSYG"
BASE COUNT          360 a          767 c          734 g          398 t
ORIGIN      
        1 gcccgggcgg cccggtccgc gtcgtccagc cgggcgaagc gcggacggcc gggcaagccc
       61 ggctcgaatc cttctcttgg gcgcagatcc tccctcgccc cgatcaggcc ggcgacgtgt
      121 tcggccaggg agggggcggc cgtcaagccg ggcgattcga tgccgccgag gttgatccag
      181 cggggatgcc ggcggctttc tgcgaccagg aaatcggccg gcccgcccgc cgccgccaag
      241 ccgtcgagct tcggccgcga gcccgcgtac gcgtggatga ccgggacccg gccgagggcc
      301 gggaccagcc cgaaggcttc ggccttgaga cggtccatga ccccgcgggt cgagcctgcc
      361 tcgtccttgt cgtcggtgga ttcaccgctc gggcccagaa ggacgggccc ctcggtcgtg
      421 ggagtcagat ggatgccgag cgaggattcg tcctgaggcg ggacgggata gacgggcatc
      481 cggaggagat cgccggcggc cttgtccaaa acgaggtatt cgcccttgaa gggacggatg
      541 cggaaatccg ccggtccggc caagccggcg acttcatcgg cctgaaggcc ggcgcagttg
      601 accacccagc gggcggagac cggtccggcc ggcgtctcga ggataaaccg gtccggccgg
      661 caatcagccg ccgtgacgcc gctgtcgaga cggacctcgg cgccgttcct ccaggcgttt
      721 tcggccaggg cgatggtgaa ttcatagggg gaaatgatgc cggtccaggg cgaaaaaaga
      781 gcccaggcgc cccggacttc cggctcgaga ttccggatcg cggccgcgcc gacgatgtcc
      841 aggccgggag ttccgttccg ctctccttgc gcccggaggg cttcgagatg ccggcggccg
      901 tcctcgtcga aggcgacgac gagctttccg gttttccggt gggggacgcc gagaccggag
      961 gcaagcccgt agatcgcccg caagccggcc gcgttcatcc gggctttaag gctccccgga
     1021 cggacgttga agccggcgtg aatgacgccg ctgttgccct tgctgacgcc ctcggccaga
     1081 tcgctccgcc gctccaaaag gacgaggcgc agccggtacc gggacagctc ccggagaacg
     1141 gcgcaaccga cgacgccgcc tccgatgacg gcgacgtcga acagcccggc ggacgaatcg
     1201 gcatccataa ggaggaacga tcataccaaa aggacttgcc ggtcgtccac gcgtaaggcc
     1261 ggtccggaag aggaacgatc cgtcgggcgg attcctcgag cgaaaagacg gaatctccaa
     1321 atgagcgata taagaaaaac cgccggttcc tcgggaccag gagccgccgg ccagaggcgg
     1381 cggacccgaa tcccgccgaa acgcggggga ggaaaaccag cgccgcgttg catcgccccg
     1441 gccgtttttg gtacattgcc tcccttcgat gagaaaaaag atcctgtttt cggcctcggt
     1501 cttccacgcc ctgaccgacg catccaccgt cgtcatcccg acgatttttc ccatcctcta
     1561 caaccgcgga ttcctgatca cccggtattc ccagatcggg ctcctctcca atctcggcct
     1621 gatcgccacc gtgctttttc aattcgtcgt ggtccggctc tcctaccgcc gggactaccg
     1681 gatcttcctc ctggccagcg gcttgggcat ttgcgcctgc ttggccttga cgccgctggc
     1741 cggctcgttt ttcgtccttc tcctgttttt cctcttgctc cgcctggccg cgagcgtcta
     1801 tcacccgctg atcatcgcct ggatctcgaa atcggcggcg gattcggaca gggagctgga
     1861 ccgggccatg ggcatccaga gcggctcggg caacgtcggg gtcatgctgg cttacttgac
     1921 ggtcggattc ctggcccagc gctggacatg gaaaactccc ctcttcgcct gggccgggct
     1981 cggcctgctt ctcctcatcg ccgtcctgct gacgctccgc ggcgttcctt cgcgaaacga
     2041 ggaaatccgg cctctccggg aggcgcagtc ctgggtccgc tccctggccg ccgtcaagcg
     2101 cttcgtcccg ggctttttct tcggaggcat gggctggaac gtgaccgtct ttttcgcccc
     2161 ctcgcttctc aaccaccgtt tcggcgtccc gatgggacag accgggctct ttctcgcctt
     2221 gtggatgggg atcggaacga tcaccggcta ctcctacgg
//
LOCUS       DAOOXH010000196         2171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_64008_length_2171_cov_3.826560, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000196 DAOOXH010000000
VERSION     DAOOXH010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64008_length_2171_cov_3.826560"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PKV64_11995"
     CDS             <1..674
                     /locus_tag="PKV64_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HPN17839.1"
                     /translation="ASIRRGGRVYMFGSGHSVIPVLDIFPRYGGFVGFFPLYDPRLMW
                     TNVIGPGGARELLWIERQEGYAKIFLQSYPLEPRDVMLVFSHGGLNAAPIDVALEAKA
                     KGLKVVSVSSHANLKIARRSHSSGKALAEVSDIAIDNCVPPEDALVDVGQTERVAAGS
                     TVAAVVVAMSLVAETAARLAETGGVPPTFVSPNVPGVPPGHMARVYQAFTEFYYARSM
                     KKEGV"
     gene            671..1861
                     /locus_tag="PKV64_12000"
     CDS             671..1861
                     /locus_tag="PKV64_12000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN17840.1"
                     /translation="MTSAPQQRPVSVGRLQASAFAGIFVFGIVMAVLGAILPSLFERL
                     RIDKAEAGGLFLLMNFAMLIVSFFFGPVVDRFGFKYFLAASALLVALSFGLLAVAPTY
                     GLIVLAVVVLGLGGGGLNGGTNALINDLHPGGQGSALNLLGIFFGFGALTIPFLIGTL
                     LAGIGLVRILFLAAAAGLVPLLMFLTLRFPEAKQPQGFPIRKAKLIAGDPLLWICAFI
                     LFFQSGNEFTAGGWISTYLQEGHGLTGAAASFVLAGYWGAIMAGRFAASRIVGRTGNH
                     PLVLGSALLSLAAAGLLVAAPSGVWAAAGALLLGLGFAAIYPTTLATAGERFASISGT
                     AFSILFTVALSGGMLGPWLAGLIAKSSSLRRGLLLPVVNCAAIIVLQVFLSRVSKKER
                     PPSV"
     gene            1890..>2171
                     /locus_tag="PKV64_12005"
     CDS             1890..>2171
                     /locus_tag="PKV64_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17841.1"
                     /translation="MSLFERKKRITAAAALAAALIFSVPASAREAGNAVSLRKKLVDG
                     HAELLMEKGILDFSGREIEGRLEELLRAGDLSSVLAALNHMPSNLGGFPP"
BASE COUNT          300 a          751 c          671 g          449 t
ORIGIN      
        1 ccgcttccat ccgccggggc gggcgcgtct acatgttcgg cagcgggcat tcggtgatcc
       61 cggtgctcga catctttccc cgctacggcg gcttcgtcgg tttcttccct ctctatgacc
      121 cccggctgat gtggacgaac gtcatcgggc cgggcggagc ccgcgagctc ctttggatcg
      181 agcggcagga aggctatgcc aagatcttcc tccagagcta cccgctcgag ccccgggacg
      241 tcatgcttgt cttttcccac ggcgggctca acgccgcccc catcgacgtc gccctcgagg
      301 ccaaggccaa gggactcaag gtcgtctcgg tctcctccca cgccaacctc aagatcgccc
      361 ggcggagcca ttcctcgggc aaagccctgg ccgaggtctc cgacatcgcc atcgacaact
      421 gcgtcccgcc ggaagacgcc ctggtcgacg tcggccagac ggagcgggtc gcagccggat
      481 cgacggtcgc ggccgtcgtc gtggccatgt cgctggtggc cgaaacggcc gcccggctcg
      541 ccgagacggg cggagtcccc ccgacgttcg tctcccccaa cgtccccggc gttccgcccg
      601 gacacatggc ccgggtctac caggcgttca cggagttcta ctacgcccgg tccatgaaga
      661 aggagggcgt atgacctccg ccccgcagca gcggccggtg agcgtcggcc gtcttcaagc
      721 ctcggccttc gccgggatct tcgtcttcgg gatcgtcatg gccgtcctgg gggccatcct
      781 tccctcgctg ttcgaacggc tccggatcga caaggccgag gccggcgggc ttttccttct
      841 tatgaacttc gccatgctca tcgtctcgtt ctttttcggc ccggtcgtcg accgcttcgg
      901 attcaaatat ttcctggcgg cgtcggccct gctcgtcgcc ctgtctttcg gcctgctggc
      961 cgtcgccccg acctacggcc tgatcgtcct ggccgtcgtc gtcctggggc ttgggggagg
     1021 gggattgaac ggcggaacca acgccttgat caacgacctt catcccggcg gacaaggctc
     1081 cgccctgaac ctgctcggga tctttttcgg cttcggggcc ctgacgatcc cgtttttgat
     1141 cggaaccctc ttggcgggga tcggccttgt ccggatcctg tttctggccg cggccgcggg
     1201 acttgtcccc ctcttgatgt ttctgaccct gcgctttccc gaggccaaac agccgcaggg
     1261 attccccatc cggaaggcca agctcatcgc cggcgatcct ctgctctgga tctgcgcttt
     1321 cattctattc tttcaatcgg gaaacgaatt cacggccggc ggatggatct cgacttatct
     1381 tcaggaggga cacggtctga ccggggccgc ggcctcgttc gtcctggccg gctattgggg
     1441 ggccatcatg gccggccgtt tcgcggccag ccggattgtc ggcaggaccg gcaaccatcc
     1501 gctcgttctc gggagcgccc tcctcagcct ggccgcggcc gggctcctgg tcgccgcgcc
     1561 ttccggcgtt tgggcggcgg ccggcgccct gctcctgggt ctcggcttcg ccgcgattta
     1621 tccgaccacc ctggccacgg ccggggagcg gttcgcctcg atctccggca cggccttcag
     1681 cattcttttc acggtcgccc tctccggcgg aatgctcggc ccctggctgg ccgggttgat
     1741 cgcgaaatcc tcttcgctgc ggcggggtct gcttcttccg gtcgtcaact gcgcggcgat
     1801 catcgttctg caagtttttt tgagccgcgt ttcaaaaaaa gagcggccgc cgtccgtttg
     1861 agaaagccgg gcggcgaagg gggaaaacga tgagtctttt cgagcggaaa aaaagaatca
     1921 cggccgcggc ggccctggcc gccgccttga ttttttccgt cccggcgtcg gcgagggagg
     1981 ccggaaacgc cgtctcgctt agaaaaaaac tcgtcgacgg gcacgccgag ctcttgatgg
     2041 aaaaagggat cctggatttc tccggccggg agatcgaggg acggctggag gagctgcttc
     2101 gagccgggga tctttcctcc gtcctggccg cgctcaatca tatgccctcc aacctcggcg
     2161 gctttccccc c
//
LOCUS       DAOOXH010000197         2162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_64415_length_2162_cov_4.826768, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000197 DAOOXH010000000
VERSION     DAOOXH010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64415_length_2162_cov_4.826768"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PKV64_12010"
     CDS             <1..424
                     /locus_tag="PKV64_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17842.1"
                     /translation="PGQYPAVIAVQSVSDEAGEGLHLQGSLTALAVITVGKARDDETS
                     EEIAVERRDGQAFLVLRRKNSGRFFAPTAGGVILWDEQGKKAWEAEFFDNPVPPLSEG
                     IYRIPLDAGLAGGRYEAECVLYRLAGKKTSLKKAVLVE"
     gene            435..>2162
                     /locus_tag="PKV64_12015"
     CDS             435..>2162
                     /locus_tag="PKV64_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17843.1"
                     /translation="MKRDRRPAMRRAVSVAGVLVLLAGLAPVARADRGSADFSIGNRS
                     HLFRNPFGGSADASETVFHFGLAQTITNYGTVSLWADGLASGGAFKPARLFAGWRGFK
                     IKNAELSAGLGDGFFVNSNLESRFVNQYHPYFYFRGLNASLSGPAFDLSVFGGTLARL
                     TGLLGSTYEIKDQPFYGIRGRWKLPAGGLAGAGIIRTRRDPADPEGPEKNTLILLDAE
                     APVVEGTKILGEFQTSLTPEPSGPAGSANALRLGPFFRSGKWNIEANYRFTGSRFCDL
                     NPDYQYVRDEQGLFASVRYQASRNLVLFGFADRFADNTGGDPAKNTLRSLSVISGFYL
                     NTRSLFDLSLQWEFQRRASEHDLPGSVDFSSNGVFLQASRTMGDFFPYLRIKLQSVRE
                     GPGLPASGLPVIYLGFRKRMPRGSFLWVEGQYDHKRNTEAGLIDRNLYVRTGWNQVFS
                     PDFDLYAELFYERYGAVQAVSQIVAYVGVRVGLGRTLQVRADIRASEPLNRRDIRSSD
                     YQFTLRLDKRFSWGQEPKIMGRKTVGAVLGIGSIEGYVFEDTNGDGLMSPGEKGLAGV
                     SLRLEDGSRA"
BASE COUNT          372 a          739 c          652 g          399 t
ORIGIN      
        1 accgggacag taccccgccg tgatcgccgt ccaatccgta tcggacgagg ccggcgaagg
       61 gcttcatctc cagggaagcc tgaccgcttt ggcggtcatc accgtgggga aagcccggga
      121 tgacgaaacg agcgaggaga tcgcggtcga gcgccgggac ggacaggctt tcctcgtcct
      181 tcgccggaaa aattccggcc ggtttttcgc cccgaccgca gggggagtca tcctttggga
      241 cgaacagggc aaaaaagcct gggaagcgga attcttcgac aaccctgtgc ctcccctgtc
      301 cgaaggcatc tacaggattc cgctcgacgc cggcttggcg gggggccgct acgaagccga
      361 atgcgtcctc taccggctcg ccgggaaaaa gacctccctg aaaaaagctg ttctcgtcga
      421 gtgagcgaga aaacgtgaag agggaccgcc ggcccgcgat gcgtcgagcc gtttcagtcg
      481 ccggcgtcct tgtcctcctg gccgggctcg cccccgtcgc ccgggccgac cggggatcgg
      541 ccgacttttc catcggcaac cggagtcatc tcttccgcaa tcctttcggc ggatcggccg
      601 acgcctcgga aaccgttttt cacttcggcc tcgcccaaac gatcaccaat tacgggaccg
      661 tctcgctctg ggccgacggc ctggcgtccg gcggcgcctt caagcccgcg cggcttttcg
      721 ccggctggcg ggggttcaag atcaagaacg cggagctgtc cgccggcctc ggcgacggct
      781 tcttcgtcaa ttccaacctc gaatcgcggt tcgtcaatca gtaccatccg tatttttact
      841 tccggggcct gaacgcctcg ctctccgggc cggccttcga cctctcggtt ttcgggggga
      901 cgctggcccg gttgaccggg ctcctgggga gcacgtacga gatcaaggac cagccttttt
      961 acgggatccg gggccgctgg aagctgccgg ccggggggct cgccggggcc ggaatcatcc
     1021 gcacccgccg ggatccggcc gacccggaag gccccgaaaa aaacaccctg atcctgcttg
     1081 acgccgaagc cccggtcgtc gaaggaacaa aaatcctggg agaatttcaa accagcctca
     1141 cgcccgaacc ctccgggccg gccggctcgg ccaacgccct gcgcctcggc ccgttcttcc
     1201 ggtcgggaaa atggaacatc gaggcgaact accgcttcac cgggtcccgt ttttgcgacc
     1261 tcaatccgga ttatcagtac gtccgcgacg agcagggcct cttcgcctcg gtccgctacc
     1321 aggcctcgcg caacctggtc ctcttcggct tcgccgaccg cttcgccgac aacaccggcg
     1381 gcgatccggc taaaaacacc ctccgctcct tgtcggtcat ttccgggttt tatctcaaca
     1441 cccgctcgct cttcgatctg tccctccaat gggaattcca gcgccgggcc tcggaacacg
     1501 atcttcccgg ctccgtggat ttttcctcca acggcgtctt cctccaggcc tcccggacga
     1561 tgggcgactt tttcccctat ctccggatca agctgcaaag cgtccgcgag ggccccggcc
     1621 ttcccgcaag cggcctgccc gtcatctatc tcggcttccg aaagaggatg ccccgggggt
     1681 cgttcttatg ggtcgagggg caatacgacc ataaacggaa caccgaggcc ggcctgatcg
     1741 accggaacct ctacgtgcgg acgggttgga accaggtctt ttcgcctgat ttcgaccttt
     1801 acgccgagct tttctatgag aggtacggag ccgtccaggc cgtctctcag atcgtcgcct
     1861 acgtcggcgt ccgggtcggc ctcggccgga ccctccaggt gcgggccgac atccgggcgt
     1921 ccgagccgtt gaaccggagg gacatccgct cgtcggacta ccaattcacc ctccgcctcg
     1981 acaagcgctt ctcctggggc caagagccta agatcatggg ccgcaagacc gtcggggccg
     2041 tcctcggcat cgggtcgatc gaaggatacg tgttcgagga cacgaacggc gacggcctca
     2101 tgagccctgg cgaaaaggga ttggccgggg tgtcgctccg tctcgaggac ggatcgcggg
     2161 cg
//
LOCUS       DAOOXH010000198         2123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_66185_length_2123_cov_5.065764, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000198 DAOOXH010000000
VERSION     DAOOXH010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66185_length_2123_cov_5.065764"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..>2123)
                     /locus_tag="PKV64_12020"
     CDS             complement(80..>2123)
                     /locus_tag="PKV64_12020"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HPN17844.1"
                     /translation="RAEIQLGRGNILRLDRETQVDLAGLPGRDGDPTKIHLIQGSVFF
                     RVRDLDREKNLQIHTPDASFYVLEAGLYRLDVSSNSRTDLSVYSGQAEAAGEDGSVLV
                     RSGEMITAAEGRWSGGPSGLFARRDDFESWNESRDALYARRLSRTYLPADYADYDYEL
                     ADYGRWCYEADYGYVWVPTIYDAGWRPYYHGRWAWYPIIGWTWVSAEPWGWCTSHYGR
                     WGWRYGLGWYWIPMHRWSWGPAWVHWYNHYDYIGWCPLSYWDYPVVIINNHFYGRHHG
                     GWDRHRDYSRSMTVVRRSQLQDRSISRIALNDRSGLDLGRISLGAAQPDIRPALERTS
                     TLAGRAREALNRGALRRVGEGFTPSAGRPAPVNGRGLRAPEIKRPDGGAASREMGTSR
                     TPYSSGRLAERGTPETPRPAAERLIRPDAGGRPNFSGEGKPAGGKEPGTMRVYPSRSS
                     SPSRLADLAKRSFSSPPRDDRPAIRKEDPSSRSSSPGIRAYPSSAPSPSRRGEPSSRS
                     SEERVRSSFPERPSSPGIRAYPSSTPPSSKDSSSSSRFNNRPSRLMSSSRSSDEPSRS
                     YTSPSRRLSSPERSYSSKIRPRFSSSPPRSSSSSPRSSDFSSRSYERPPRSSSSSRSY
                     NAPSRSSSSSSSRSVSRSSSSSSSSRSSGKPSRSSSSSSKSSSSSGSKSSGSPRRR"
BASE COUNT          419 a          646 c          752 g          306 t
ORIGIN      
        1 ccgtcgctgt ctgcgattgt aagcccaaaa aaaacgctct gtcaaatctt tttttgagcc
       61 tttccgcccc ccccgcggcc taccgccggc ggggggaacc cgatgatttc gagccgctcg
      121 aggaggagga cttcgaagaa gacgaggagg accgggacgg cttcccggat gagcgcgagg
      181 aggaggaaga agaggaggat ctcgagacgg accgggagga agaggaagaa gagctccggg
      241 agggcgcatt atacgagcgg gaagaggaag agctccgtgg cggcctctcg tacgaccggg
      301 acgaaaaatc agaggagcgg ggcgaagagg aggagcttcg cgggggggag gaagagaatc
      361 gcggccggat tttactcgaa taagaccgct ccggggaaga caagcgccgg gacgggctgg
      421 tataagagcg ggacggctcg tcggacgaac gggaggagga catgagccgc gacggccggt
      481 tattgaaccg ggaggaagaa gacgagtcct tcgaggacgg aggagtcgag ctcggatacg
      541 cccggatccc cggcgaggac ggcctttcag ggaacgagga gcggacgcgt tcttcggaag
      601 aacgggaaga aggttccccc cgccgcgacg gcgaaggagc cgagctcgga tacgcccgga
      661 tccccggcga ggaagaccgg gaggaaggat cttctttccg aatcgccggg cggtcgtcgc
      721 gcggagggga ggagaaggag cgcttggcca gatcggccag gcgggacggc gaggaggatc
      781 gggagggata aaccctcatc gtccccggtt cctttccgcc ggccggcttt ccctctccgg
      841 aaaagttcgg ccgcccgccg gcatcgggac ggatcaggcg ttcggcggcg ggacggggcg
      901 tttccggcgt tccccgctca gccagacgtc cggacgaata gggcgtgcgg ctggttccca
      961 tctcgcggga agccgcgccg ccgtccggcc gcttgatttc cggggcccga aggccccggc
     1021 cgttcacggg cgccggccgt ccggccgagg gagtaaagcc ctcgccgacg cggcggagcg
     1081 cgccgcggtt gagcgcctcg cgggcccggc cggccaaggt cgacgtccgt tcgagggcgg
     1141 gccggatgtc gggctgggcg gccccgagcg aaatccggcc gaggtcgagg ccggaccggt
     1201 cattaagggc gatccgggaa atggaccggt cttgcagctg gctccggcgg acgacggtca
     1261 tcgaccggga gtagtcccgg tgacggtccc atccgccgtg atgccgtccg tagaaatggt
     1321 tgttaatgat gacgacaggg taatcccaat agctgagggg gcaccagccg atgtaatcgt
     1381 aatgattgta ccagtgaacc caagccggcc cccacgacca gcgatgcatc ggaatccaat
     1441 accaacccag gccgtagcgc cagccccacc ggccgtaatg cgaggtgcac cagccccagg
     1501 gctcggcgga cacccacgtc cagccgatga tcggatacca ggcccagcgg ccgtgataat
     1561 acggccgcca gccggcgtcg taaatcgtcg gcacccaaac atagccgtag tcggcctcgt
     1621 agcaccaccg gccgtagtcc gccaactcat agtcatagtc ggcataatcc gcgggcagat
     1681 aagtccggct caagcgccgg gcgtacaatg catcgcgcga ttcgttccac gactcaaaat
     1741 cgtcccggcg ggcgaacagg ccggacggcc ctccgctcca ccggccttcg gcggccgtaa
     1801 tcatttcccc cgagcggacg agaacggatc cgtcctcacc ggccgcttcg gcctggccgc
     1861 tgtacacgga gaggtccgtg cggctgttcg acgagacgtc cagccggtac aggccggcct
     1921 caaggacgta aaaggacgcg tcgggcgtat ggatctgaag atttttctcc ctgtccaaat
     1981 cccggacccg gaaaaagacg ctgccctgga taaggtggat tttggtggga tcgccgtccc
     2041 tcccggggag cccggcgagg tcgacttggg tctcccggtc gaggcgcaga atgttgcccc
     2101 ggccgagctg gatttccgcc cgg
//
LOCUS       DAOOXH010000199         2069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_68775_length_2069_cov_6.587388, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000199 DAOOXH010000000
VERSION     DAOOXH010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68775_length_2069_cov_6.587388"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="PKV64_12025"
     CDS             <1..993
                     /locus_tag="PKV64_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HPN17845.1"
                     /translation="GTCRRLRAGENPGANLGIILGTLARAGRDKAVFLMSAPLQDFGA
                     WVEQLLAESTGKEGQGILPIVRPTAAPSSQPAGDRLTVAIGLAGDAALAESVNKARER
                     GEPVISMTLEDPIELGAQFFLWEFATAVAGTVLGINPFDQPNVALSKKKTEAALNVFM
                     KSRTFPEESSTWEEETLAFYGEKREVGLRSGFRTLFNKFRDGGYAAIQAFLPPSPETT
                     EALRTLAGRIETRTGRPVVFDYGPRFLHSTGQLHKGDGGAGIFIQLTGVHPKDTAVPS
                     SPEGGGSAYSFGNLIDAQALGDRQALREKGRPVARIHFKQDPVEGISKAVERLF"
     gene            complement(1202..1951)
                     /locus_tag="PKV64_12030"
     CDS             complement(1202..1951)
                     /locus_tag="PKV64_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17846.1"
                     /translation="MMKFVGLSLTALLLLTSAVACGKKAAEPAAIEGAVAETTESGLS
                     IVIGLNTVEGTVKNAKGSYFFMDELPGFDVCVTSPVEGGDAAALIGKPVRVKGLFNKD
                     LPNLLVAQSIEIKEGEERFRSVYTSTDASAPQDFFNQKSRDEYPPLALTKFDKNADWE
                     GKGKGKVFGTLIPGTAGAADRISIMGADGKEVGKVIVDSKSTFAEFYAGKLKLFDKVW
                     FYLNIKDSVDKALRAKSKEMFHADVVFIGLY"
BASE COUNT          411 a          646 c          621 g          391 t
ORIGIN      
        1 ggaacctgcc gccgcctccg ggccggggaa aatccaggag cgaacctggg gatcatcctg
       61 gggacgctgg cccgggcggg ccgggacaag gcggttttcc tgatgtccgc ccccctccaa
      121 gatttcgggg cttgggtgga acagctcctg gccgaaagca cgggaaaaga aggtcaaggg
      181 atcctgccga tcgtccggcc gacggccgct ccatcttccc aaccggccgg cgaccgcctg
      241 acggtcgcca tcgggctggc cggcgacgcg gccctggccg agtccgtgaa taaagcccgg
      301 gaacgcggcg agccggtgat ttcgatgacc ctcgaggacc cgatcgagct cggggcgcaa
      361 ttcttcctct gggaattcgc cacggccgtc gccgggaccg tcttggggat caatcccttc
      421 gaccagccga acgtcgcgct ttccaagaaa aaaacggaag cggccttgaa tgtctttatg
      481 aaaagccgga cgtttccgga agaatcctcc acctgggagg aagaaaccct cgccttctac
      541 ggggaaaaac gggaagtcgg actccgctcg ggatttcgga cgctgtttaa taaattcagg
      601 gacggcgggt acgccgcgat ccaagcgttc ctccccccct cccccgaaac cacggaagcc
      661 ctccggacgc tggccggccg catcgagacg cgcacgggac ggcccgtcgt cttcgactac
      721 ggcccccgtt tccttcattc caccggccag cttcacaagg gagacggcgg agcggggatc
      781 ttcatccaac tgaccggagt ccaccccaag gacacggccg tcccctcctc gcctgaaggc
      841 gggggatccg cttattcctt cggaaatctg atcgacgccc aagccctggg cgaccggcag
      901 gcccttcggg aaaaaggccg gcccgtggcc cgcatacact tcaagcagga cccggtcgag
      961 gggatttcca aagccgtcga gcgcttgttt tgagcccgcc gggatcaatt cctgtttttg
     1021 agaaaacgct ttttcctgtc gggagggcgg atatcgtgaa ggcggggacg gccgggcatc
     1081 cattgtccgg gcgatgcttt tcttcttgag acatcttcga gaaaccagcc cggctgaaaa
     1141 aaaaggccgg ttcccctgcg ggggaaccgg cctgaaaaat cagcggatgc gtccggccgg
     1201 ctcagtagag cccgatgaag acgacgtcgg catggaacat ctccttgctc ttggcccgga
     1261 gcgccttgtc cacgctgtcc ttgatgttca gatagaacca gaccttgtcg aacagtttca
     1321 gctttccggc atagaattcc gcgaaggtcg atttcgaatc gacgatcacc ttgccgactt
     1381 ccttcccgtc ggcgcccatg atcgaaatgc ggtcggcggc gccggcggtc ccgggaatca
     1441 gggttccgaa gaccttgcct ttccccttgc cttcccaatc cgcgttctta tcgaacttcg
     1501 tcagggcaag agggggatat tcgtcccggg acttttggtt gaagaagtcc tgaggggcgg
     1561 aggcgtcggt cgaggtgtaa acgctccgga accgctcctc gccttccttg atttcgatcg
     1621 actgggcgac aagaaggttg ggcaggtcct tattgaagag gcccttgacc cggaccggtt
     1681 tcccgatcag agccgccgcg tctccgccct cgacggggct cgtgacgcag acatcaaacc
     1741 cgggaagttc atccatgaag aagtagcttc ccttggcgtt tttgaccgtt ccttccaccg
     1801 tgttcaagcc gatgacgatc gacaacccgg actcggtcgt ttcggcgacg gcgccttcga
     1861 tcgccgccgg ttcggcggct tttttgccgc aagcgaccgc cgacgtcagc agaaggaggg
     1921 ccgtgaggct caggccaacg aatttcatca tatcggcctt atgaaacata gcgcatacct
     1981 ccaaagaaga gtcgatccac cgcgatacgg gccggaatta taacaccgcg gggaggaaaa
     2041 agcaaagcgg ctccattgac aggatttcc
//
LOCUS       DAOOXH010000200         2065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_68950_length_2065_cov_6.262687, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000200 DAOOXH010000000
VERSION     DAOOXH010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68950_length_2065_cov_6.262687"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PKV64_12035"
     CDS             complement(<1..208)
                     /locus_tag="PKV64_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPN17847.1"
                     /translation="MKRVLTLSLAGLVLAALAAAGPITLDVKEQTLGNGMKILVIPNP
                     GVPRVVCHVYYKVGSINERPGITGI"
     gene            complement(566..>2065)
                     /locus_tag="PKV64_12040"
     CDS             complement(566..>2065)
                     /locus_tag="PKV64_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17848.1"
                     /translation="IAALTETYGQFGSGLPGQKLEGAYDNSYLVADPREAWILETAGT
                     RWAAKRIEGGTVSISNTLSLGGSLDLSSADLSAHAREKGWWTGSPEAAFSFERAYAAE
                     GRDQEIARGRAQVRANCSLGLLREKSGSIDESWMKRIARDRSTDPSLDLDATASSCVA
                     SLPADGGGLPVFWWCASVPSSGVFVPFFVHGTELPVFVSAAGTAGKRVVRPETAPVDR
                     YSPDSYWWLFRDLTDLVNLDRPGRLAAVRKEFDALEQSFAAALPPVLKSAAELRKAGK
                     TAEAARVLDDFSAACVESAAAAARALRDSWKPAGSDESAEPEEAGVYIANFGAFADAE
                     WNVSARDGRLFLEIPGQGALELRPPDAKGFWALAASPQAGVSFSRRPEFGVTAMIFRR
                     GAMSFELPRKGIVLPPEIPLEELRKFLGEYHGDELDETLEIVIKNNSLALKIPGQKTY
                     ELRPPDAEGKRFFRVAPLVYLVFKESETGGVESFTYHQGPSALTYKKIK"
BASE COUNT          388 a          672 c          646 g          359 t
ORIGIN      
        1 cgatgccggt gatgccgggc cgctcgttga tcgatcctac cttgtaatag acatgacaga
       61 cgacacgggg cacgcccgga ttggggatca ccaggatttt catcccattc cccagggtct
      121 gctccttgac gtccagggtg atcggcccgg ccgcggcaag agcggccagg acgagccccg
      181 cgagcgaaag cgtcaagacg cgcttcatgc gggtcctcct cctcgtgaaa ttaaattggc
      241 cgggagccgg ccgggacggc cggaaaaacg gaaatcgttc tcccttctta ttagacgccc
      301 cggactccgc cttcttccat catcggaagc ggcctgataa ccggtccccc ccctcatggg
      361 agggacgata aaatctaaaa cgatccgcaa tgagacgtcc cggcggcgtt tcctccggcc
      421 ggctgagaca cgattgtcgg cctcatcgaa aaacggaaaa ggcgcgggcc gccggagaga
      481 gcggcggcga aatgccgggg aaagccctcc cggcgcggcg ttcttgagtg aaatcaatga
      541 attcccgggg tctcgactcc tcgaatcatt tgattttctt gtaggttaac gccgacgggc
      601 cttgatggta agtgaacgac tcgacgcctc ccgtttccga ttctttaaaa accaggtaaa
      661 ccaagggcgc gacgcggaaa aatcgttttc cctccgcatc cggggggcgc agctcatagg
      721 ttttctgccc ggggattttc aacgccaggc tgttgttctt aatgacgatc tccagggttt
      781 cgtcgagctc gtcgccgtgg tactccccaa gaaatttccg gagctcttcc aggggaatct
      841 ccggcggcag gacgattccc tttcgcggca gctcgaagga catcgccccc cggcggaaga
      901 tcatggccgt gacgccgaat tcgggccgcc ggctgaagga aacgccggct tggggagagg
      961 cggccagggc ccaaaaaccc ttagcatccg gcggacgaag ctcaagggcg ccctgtcccg
     1021 ggatctccag gaacagacgg ccgtcccggg ccgagacgtt ccattctgcg tccgcgaaag
     1081 ctccgaaatt ggcgatgtag acgccggcct cttccggttc cgccgattcg tctgagccgg
     1141 ccggtttcca ggaatcccgg agcgcccggg ccgcggccgc ggcgctttcg acgcaggcgg
     1201 ccgagaaatc atccaacacc cgggccgcct ccgcggtttt tccggccttc cgcagttccg
     1261 cggccgactt gaggacgggg gggagggcgg cggcgaagga ctgttccaag gcgtcgaatt
     1321 cctttcggac ggccgcgagc cggcccggcc ggtcgagatt gacgaggtcg gtcaagtctc
     1381 ggaagagcca ccagtaggaa tcgggcgaat atcggtcgac cggggccgtc tccggccgga
     1441 cgacgcgctt ccccgccgtt ccggccgccg agacgaagac cggaagctcc gttccatgaa
     1501 cgaagaacgg gacgaaaacc ccgctcgagg ggacggacgc gcaccaccag aaaacgggga
     1561 gcccgccgcc gtcggcggga agcgaggcga cgcaggagct ggccgtcgcg tccaggtcca
     1621 ggctcgggtc ggtcgaccgg tcgcgggcga tccgcttcat ccaagattcg tcgatcgatc
     1681 cggatttttc ccggagcagc ccaagggaac agttcgcccg gacctgagct ctcccccggg
     1741 cgatttcctg atcccgccct tcggccgcat acgcccgctc gaagctgaac gcggcttccg
     1801 ggcttcccgt ccaccagcct ttctcccggg catgggccga aaggtcggcc gacgacaagt
     1861 ctagggagcc gcccagactc agggtattgg agatcgagac cgttcccccc tcgatcctct
     1921 tcgccgccca gcgggttccg gccgtttcca aaatccaggc ttccctcgga tcggcgacga
     1981 gataagaatt gtcatacgcg ccctccagct tttggccggg aaggccggag ccgaactgcc
     2041 cgtacgtttc cgtcaaggcc gcgat
//
LOCUS       DAOOXH010000201         2059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_69250_length_2059_cov_7.321856, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000201 DAOOXH010000000
VERSION     DAOOXH010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69250_length_2059_cov_7.321856"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PKV64_12045"
     CDS             <1..191
                     /locus_tag="PKV64_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17849.1"
                     /translation="AIVAFGEGFPLSLHEGQKLADGFVLSIEADRVVFRQTSDKGIPL
                     AKPKDIAKEISPEEPAHD"
     gene            184..>2059
                     /locus_tag="PKV64_12050"
     CDS             184..>2059
                     /locus_tag="PKV64_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HPN17850.1"
                     /translation="MTKTPIRLTLAVVGLAIAVAGFSQTGSSPAPLRVTILPTRVNVR
                     VILDGAAFPAGISPAYAAGNEAVLTAELPKGSTLAAVPLIPADEPALVRAAKLGPAAD
                     GRTLLSLTLAERVPVRCWTEETKTVIELIRIQRGEDYLIAPDTKAELDRTARSVQPLA
                     ISTDADAGNRFDLNVRQGRRAIINTFALDNPLRLVVDVFDAVLGQPPAEVYVGRHGVE
                     KFRTGQFKKGDPYSISRIVFDLREPRLFALAAADDGVSVVFADAARPIAAVSGGPTAA
                     ADSALEKVIAVKNAAPPPSAAAPAPVPTEAKNPVAVSVPASPIQEKSGPKAEPKAEVP
                     APVVSPAPAKVEPKADPPAAPPAVKPEPKAETRTVERQIPVTIVPPPEPAEKADVQPP
                     IQAQEEPVATAPPPQERAASRTIHDPVEKYSGELISPKFKDADLRDVILWLGGRVGLN
                     VIIDQDVRGRVTVSFEDVPWDQFLDMILKNNKLGRILEGNVLRIAPLNVLADEEKAQL
                     ALMTAREASGPIITKTYNLSYATARDVNDIIKSKKSARGDIVIDTRTNSLIVSDVQEK
                     IDLIDEIVATLDAATPQVTIETRIVEATSTFVRNLGIQWGYKAIADPFYGNQTSLQFP
                     N"
BASE COUNT          388 a          748 c          604 g          319 t
ORIGIN      
        1 aggccatcgt cgcgttcggc gagggattcc ccttgtcgct gcacgaaggc caaaaactcg
       61 ccgacggctt tgtcctctcc atcgaagccg accgcgtcgt tttccgccag accagtgata
      121 aaggaatccc gcttgccaag cccaaagata tcgcgaaaga gatctcgccc gaggagcccg
      181 ctcatgacta agacccccat ccggctcacc ctagccgtcg tcggactcgc gattgcggtc
      241 gccgggttct cccagacggg atcttccccc gcgccgcttc gggtcaccat cctgccgacc
      301 cgggtcaacg tccgtgtgat cctggacggc gccgcctttc cggcggggat atccccggcc
      361 tacgccgcgg ggaacgaggc cgtgctcacg gccgagctgc cgaagggttc gaccttggcc
      421 gccgtccccc tgatcccggc cgacgaaccc gcccttgtcc gggcggccaa gctcggtccg
      481 gcggccgacg gccggacgct tctgtccctg actctggctg agcgggttcc tgtccgctgc
      541 tggacggagg agactaaaac cgtcatcgaa ttgatccgga ttcagcgcgg agaagattat
      601 ctcatcgccc ccgataccaa ggccgagctc gaccgcaccg cccggtccgt tcaaccgctg
      661 gcgatttcga ccgacgcgga cgccgggaac cggttcgatc tcaacgtccg gcagggccga
      721 cgggcgatca tcaatacctt cgccctggac aatccgctgc gtctcgtggt cgacgttttc
      781 gacgcggtct tggggcagcc cccggccgaa gtgtacgtcg gccgccacgg agtggaaaaa
      841 ttccggaccg gccagttcaa aaaaggcgat ccctattcca tcagccggat tgtctttgac
      901 ctccgggagc cgcggctctt cgccctggcc gccgcggacg acggagtgag cgtcgtcttc
      961 gccgacgccg ctcggccgat cgccgcggtt tcgggcggcc caaccgcggc cgccgactcg
     1021 gctctcgaaa aagtcatcgc cgtgaaaaac gccgccccgc cgccgtccgc ggcggcgccc
     1081 gctcccgtcc cgaccgaagc gaaaaatccg gtcgcggtct cggtcccggc ctccccgatt
     1141 caggagaaat ccgggccgaa agccgagccc aaggccgagg ttcccgcccc ggtcgtcagc
     1201 cccgcgccgg cgaaagtcga gccgaaggcc gatcccccgg ccgccccgcc cgccgtgaag
     1261 ccggaaccga aggcggaaac ccggacggtc gaacggcaga tcccggtgac gatcgttccg
     1321 ccccccgaac cggctgaaaa agcggacgtc cagcccccga tccaagcaca ggaagagccg
     1381 gtcgcgaccg cccccccgcc gcaggagcgg gccgcctccc ggaccatcca tgatccggtg
     1441 gagaaataca gcggcgagct catcagcccg aagttcaagg acgccgacct ccgcgacgtc
     1501 attctttggc tgggcggccg ggtcggactg aacgtcatca tcgaccagga cgtccggggc
     1561 cgggtgacgg tctccttcga ggacgtcccc tgggatcaat ttctcgacat gatcctgaaa
     1621 aacaataagc tcgggcgaat cctcgagggc aacgtcctcc ggatcgcccc cctcaacgtc
     1681 ctggccgacg aggaaaaagc ccagctcgcc ctgatgacgg cccgggaagc ctccggcccg
     1741 atcatcacca agacgtacaa tctctcctac gccacggccc gggacgtcaa cgatatcatc
     1801 aagagcaaga agtcggcccg gggcgacatc gtcatcgaca cccggaccaa ctccctgatc
     1861 gtttccgacg tgcaggaaaa aatcgatctc atcgatgaga tcgtcgctac cctggacgcg
     1921 gccacgcccc aagtcaccat cgagacgagg atcgtcgagg cgacgtccac gttcgtccgc
     1981 aacctgggca ttcaatgggg ctacaaggcg atcgccgatc ctttctacgg gaaccagacc
     2041 tcgctccagt tccccaata
//
LOCUS       DAOOXH010000202         2053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_69614_length_2053_cov_2.556056, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000202 DAOOXH010000000
VERSION     DAOOXH010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69614_length_2053_cov_2.556056"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="PKV64_12055"
     CDS             complement(<1..1168)
                     /locus_tag="PKV64_12055"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPN17851.1"
                     /translation="MMNHRFSSAALRRSFFPPLLALLLLFAFGCKRAEAPFRFIDRLA
                     EENVAASPLKTPSSAADSTAPGPEWAESMLAAAVVHDPPAGKNPWSLEKTLVLGPDVF
                     PVLLAAPPTELRYRLRIPPRGALRFGYGLYDENKGESAGETVFEITLEDGSSPIRIFE
                     EPLNLGRAKSHYRAAFREIDLSTRAGKTVTVVFRTRAAEVLEPKDARAFSEKNGVAFW
                     IDPLLVPRREAGKPAAEPPNIILISIDTLRADHLCSYGYRRPTSPAAERLAGDGILFT
                     SCYSAAPYTLASHATMLTGLDPARHQALYMADGITPAVPTIAEILRRAGYETAAFTGG
                     GQLNAKYGFARGFDLYNEQAGRNQLLNSAANVRRNAALWLEARKASNFFLFLHAY"
     gene            complement(1445..>2053)
                     /locus_tag="PKV64_12060"
     CDS             complement(1445..>2053)
                     /locus_tag="PKV64_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17852.1"
                     /translation="GFMVMPGRYTVSAAKVVDNVVTPLGEPQSFTVTPLADATMAAAD
                     RAGLAAFQKNVAELTRAAYAADEETAALAARVRRIRQTAAGLAGAPDSLMPAVLRAEK
                     TIDEIRLALDGYEAKASFEEVPPAPMPIRNRVEELIGIQFSTTAAPGKTQIENYRAAR
                     EDLEALIGKLETLKTVSLPALEKTLDEHRAPWTPGRIVKIGE"
BASE COUNT          350 a          619 c          688 g          396 t
ORIGIN      
        1 gataggcgtg gagaaacagg aaaaaattcg aggccttgcg ggcttccagc caaagagcgg
       61 cgttccggcg gacgttggcg gcggaattga ggagttgatt gcgtccggcc tgttcattgt
      121 acaagtcgaa tccccgggcg aagccgtatt tggcgttgag ctgcccgccg ccggtgaagg
      181 ccgcggtttc gtatccggcc cggcggagga tttcggcgat cgtcggaacg gcgggcgtta
      241 ttccatcggc catgtaaagg gcttggtggc gggccgggtc caagcccgtc agcatcgtcg
      301 cgtgggaggc cagggtgtaa ggcgcggcgc tgtaacaaga ggtaaagagg attccgtcgc
      361 cggccaggcg ttcggccgcc ggactcgtcg gccgccggta gccgtacgaa caaagatggt
      421 cggcccggag cgtgtcgatc gagatcagga tgatattggg cggctcggcc gccggtttcc
      481 ccgcctcccg tctcgggacg agaaggggat cgatccagaa ggccacgccg tttttttcgg
      541 aaaacgcccg ggcgtctttc ggttcgagaa cctccgccgc gcgggttcgg aaaacgaccg
      601 tgacggtttt ccccgcccgg gttgaaagat cgatttcgcg gaacgcggcc cgataatggc
      661 ttttcgcgcg gccgagattc agcggttcct cgaaaatccg gattggagaa gaaccatcct
      721 ccagcgtgat ttcaaagacc gtctctcccg cgctttctcc cttgttctca tcatagagcc
      781 cgtatccaaa ccggagagcg cctcggggcg gaatccgcag gcgataccgg agttccgtgg
      841 gaggagcggc cagcagaacg ggaaaaacat ccggtccgag aacgagcgtt ttctccagcg
      901 accagggatt cttccccgcc ggagggtcgt ggaccacggc ggcggccagc atggattccg
      961 cccattccgg acccggcgcc gtcgaatccg ccgcggagga tggcgttttc aagggcgagg
     1021 cggcgacatt ttcctcggcc agccggtcga tgaaccggaa cggcgcctcc gctcgtttgc
     1081 agccgaaggc aaaaaggagc aaaagggcaa gcagcggcgg gaagaaggaa cgacgtaaag
     1141 cggcgctcga aaatcgatga ttcatcatcc ccctcgtcat ttttttcttt gtcttggaca
     1201 ggctcgtttt ctttctcttg tcccttcgac taacgaaaaa gccggccggt tcattcagac
     1261 gaagaaattc tagcggggat cttccgtgct ttcaagtcca tgacccgttc cgcccggccg
     1321 gaagaacctc cgctttccgt tttgtcgagc ggggatcatc agccggaggg cttccgcctc
     1381 tctttcggga gaaccgcccg gaggggcgat tctcccgagt cgtccggcct cagatcccgg
     1441 tgcgtcattc tccgattttc acgatccgtc ccggcgtcca cggcgcccgg tgctcgtcca
     1501 gcgttttttc cagggcggga aggctcacgg tcttgagcgt ttcgagcttc ccgatcaagg
     1561 cctcgagatc ctcccgggcg gcccggtagt tttcgatctg ggttttgccc ggcgcggcgg
     1621 tcgtggagaa ctggatgccg atcagctcct cgacgcggtt gcggatgggc atcggggccg
     1681 gcgggacctc ctcgaagctg gctttggcct cgtagccgtc cagggccaaa cggatttcgt
     1741 cgatcgtttt ttcggcccga aggaccgccg gcatcaggct gtcgggagcg cccgcaagtc
     1801 cggccgccgt ctggcggatc cggcggacgc gggcggccag agccgccgtc tcctcgtccg
     1861 cagcgtaggc cgcgcgggtc agctcggcca cgtttttctg gaaggcggcc agcccggccc
     1921 ggtcggcggc ggccatggtg gcgtccgcca ggggagtgac ggtgaaggac tggggctctc
     1981 cgagcggcgt gacgacgttg tcgacgacct tggccgcgga gaccgtgtag cgtccgggca
     2041 tgaccatgaa gcc
//
LOCUS       DAOOXH010000203         2002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_72161_length_2002_cov_4.339497, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000203 DAOOXH010000000
VERSION     DAOOXH010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72161_length_2002_cov_4.339497"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PKV64_12065"
     CDS             <1..246
                     /locus_tag="PKV64_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17853.1"
                     /translation="DGVFCDACAPSRKEEIRENLGRFLSWVRDNPPSACASLPFSSEE
                     IRSFAGALQAMIIFHLEREPKSRRWLQAENGLSGRKE"
     gene            complement(227..1096)
                     /locus_tag="PKV64_12070"
     CDS             complement(227..1096)
                     /locus_tag="PKV64_12070"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HPN17854.1"
                     /translation="MGDLLKSLRPGQWTKNLFVFAALLFGREALIGSSVLRAAGAFAV
                     FCLLSGAMYLVNDVCDLAGDRLHPKKRLRPIASGRLSTRRALAAAVILAAAALAGAFV
                     LRPRLAVVAAFYAALMLAYSLRLKNVVILDVFLIASGFILRVVAGGVVLGVPLSSWLI
                     ICTSLLALFIGFGKRRHEIVFLEADASLHRSILREYSPLLLDQMISVVTASTVIAYSL
                     YTVSEETVRKFGTSNLIFTMPFVLYGLFRYLYLVHQRGQGGNPEEMIFRDKPLAATII
                     LWIASVVFILYVR"
     gene            1210..>2002
                     /locus_tag="PKV64_12075"
     CDS             1210..>2002
                     /locus_tag="PKV64_12075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN17855.1"
                     /translation="MSGGKTRTIRTASTAVFTVLALLLVFNFADQSLQSPLLDPLLSD
                     FFGRTADIVPLGWVTFVFTLCAAAATIAAGLLAGRGSRLRLCLAGSLTYGAIAVLSVL
                     IPHGRTGYAVFFLARALAGFGIGLIVPSVFALASDLVAADRRSTAFGVLSVAMLAGRL
                     AGFAAGGAFAANWRTAYALIGAINLALAAALASLREPPRGIREPELREAILAGAVYRF
                     RWTRKDAGALRAAPSNLWLIFNFVDVIPGSIILFLIFKYMKDVHNM"
BASE COUNT          356 a          660 c          645 g          341 t
ORIGIN      
        1 gacggcgttt tctgcgacgc ctgcgcgccc tcgcggaagg aagagatccg ggaaaacctg
       61 ggccggttcc tgtcctgggt ccgggacaac ccgccctccg cctgcgcctc cctcccgttt
      121 tcgtccgaag aaatccgctc cttcgccggc gccttgcagg cgatgatcat tttccacctg
      181 gagcgggagc cgaaaagccg tcgctggctt caggctgaaa acgggctcag cggacgtaaa
      241 gaataaaaac gacggaggcg atccaaagga tgatggtcgc ggccagcggc ttgtcccgga
      301 agatcatctc ctcgggattc cccccctgcc cccgctggtg gacgaggtaa agataacgaa
      361 acaggccgta gaggacgaac ggcatggtga agatcaggtt cgacgtcccg aatttgcgga
      421 ccgtctcctc ggaaacggtg tagaggctgt aggcgatcac cgtcgaggcg gtgacgaccg
      481 aaatcatctg gtcgagaaga agggggctgt attcccggag aatggaccgg tggagagacg
      541 cgtccgcctc caggaagacg atctcatgac ggcgcttgcc gaaaccgatg aagagagcca
      601 gaagcgaggt gcagatgatc agccaggagg acaggggaac gccgaggacc accccgccgg
      661 cgacgacccg gaggatgaat ccggaggcga tgaggaaaac gtccaggatc accacgtttt
      721 tcagccggag cgagtaagcc agcatcaggg ccgcatagaa ggcggccacg accgccagcc
      781 gcggacggag aacgaacgct ccggccaggg cggcggcggc caggatcacg gccgcggcca
      841 gcgcccgccg cgtcgaaagg cggccggagg cgatgggtcg aaggcgcttc ttcggatgga
      901 ggcggtcgcc ggcgagatcg cagacgtcgt tgacaagata catcgccccc gacagcaggc
      961 agaaaacagc gaacgctccg gcggcccgca ggacggagga gccgatcagc gcttcccggc
     1021 cgaacaggag ggcggcgaag acaaaaagat ttttcgtcca ttggcccgga cggagggatt
     1081 tcaagaggtc gcccatcgga agctcgtttg ccgcggttat tatatgccag ccggccgcaa
     1141 ggcgcaaagg ccccggctct tttcaatctc cgcgcttttc gctatagtgg acagcctcgg
     1201 agggcatgca tgagcggcgg aaaaacgagg acgatccgga cggcttcgac ggccgtcttc
     1261 acggtcctcg ctcttcttct tgtcttcaat ttcgccgatc agtccctcca gagcccgctc
     1321 ctcgatccgc tcttgtccga ttttttcggg cggacggccg acatcgtccc gctcggctgg
     1381 gtgacgttcg tcttcactct atgcgccgcg gccgcgacca tcgccgccgg cctgctcgcc
     1441 gggcgcggct cccgcctccg tctctgcctg gccggctcct tgacgtacgg cgcgatcgcc
     1501 gtcctgtccg tcctgatccc ccacggccgg acgggatacg ccgtcttttt tctcgcccgg
     1561 gccctggccg gtttcggaat cgggctgatc gttccctcgg ttttcgccct ggcctcggac
     1621 ctcgtcgccg ccgatcgccg gtcgacggcg ttcggcgtcc tctcggtggc gatgctggcc
     1681 ggccgcctgg ccggcttcgc cgccggcggc gcgttcgccg cgaactggcg gaccgcctac
     1741 gccctcatcg gggcgatcaa tctcgccctg gccgcggccc tcgcctcgct ccgagagcct
     1801 ccgcggggaa tccgcgaacc cgagctgcgg gaggcgattc tggccggggc cgtctaccgg
     1861 ttccgctgga cgcggaaaga cgcgggcgcg cttcgggccg ccccctccaa cctctggctg
     1921 atcttcaatt tcgtcgacgt catccccggc tcgatcatcc tgttcctgat cttcaaatac
     1981 atgaaggacg tccacaacat gg
//
LOCUS       DAOOXH010000204         1955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_74771_length_1955_cov_5.577368, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000204 DAOOXH010000000
VERSION     DAOOXH010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74771_length_1955_cov_5.577368"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /gene="ligA"
                     /locus_tag="PKV64_12080"
     CDS             complement(<1..866)
                     /gene="ligA"
                     /locus_tag="PKV64_12080"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF013790.2"
                     /note="this protein catalyzes the formation of
                     phosphodiester linkages between 5'-phosphoryl and
                     3'-hydroxyl groups in double-stranded DNA using NAD as a
                     coenzyme and as the energy source for the reaction;
                     essential for DNA replication and repair of damaged DNA;
                     similar to ligase LigB; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPN17856.1"
                     /translation="MDRSAAAAIDRLRKKIAYHEKKYYQDNDPQISDAEFDAMMRELR
                     RLEEAHPELIVPESPTQRVGGQPVEGFPSVRHRTPMLSLDNVYSVEEFEEFESRLLRL
                     LPGRTISYVAELKIDGLSVSLLYRNGRLIQAVTRGDGIQGDDVTANARTIRTLPLTIP
                     ERREVEVRGEIYLPFTSFQEINRAREAREEPLFANPRNAAAGSIRLLDPRVVAARRLD
                     MFLYYLTIEGKEPASQWEELALIRKLGFKTNPHSRRCPDAAAVVAYWREWTERRETLD
                     YDADGVVIKVDD"
     gene            complement(980..>1955)
                     /locus_tag="PKV64_12085"
     CDS             complement(980..>1955)
                     /locus_tag="PKV64_12085"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPN17857.1"
                     /translation="IIQYQSKQYNAETIGLAEKAAASGKIDDQMKGAMYQIVADIHLK
                     TNQFDKAKAAAQQAIQHANAAKGKEGEAANAKMWTQTIGQAYVLTAKAQEKAGDIKGA
                     IDSFNHAYTTLKSPAVLGELKKIGKGYYDAQKYAQAEPIFRYLAGTGLDADSMTILAQ
                     ILNKQGKEAEALQMFKDMYAKNKTGSMAYNIGIMLANEAKTNPALVNDAVAFLLDASF
                     LDTKRSKQCTDIARSLFFGQDKEWNDRIKMIDESQALIKEWTDAINSKYGDKSEEDLT
                     PDERREFRKLKELIDKETVAVNNLVAQMKAGSSKFDALVASAKKRVGK"
BASE COUNT          305 a          566 c          618 g          466 t
ORIGIN      
        1 tcgtcgtcca ccttgatgac gacgccgtcg gcgtcgtaat ccagcgtttc gcgcctctcg
       61 gtccattccc gccaataggc gacgaccgcg gccgcgtcgg gacagcgcct cgaatgggga
      121 ttggttttga agccgagctt ccggatcaac gccagctcct cccactggga ggccggctcc
      181 tttccctcaa tcgtcaggta ataaaggaac atgtcgaggc ggcgggcggc gacgacccgg
      241 ggatcaagaa ggcggatgga gccggcggcg gcattgcgcg ggttggcgaa gagaggctcc
      301 tcgcgggcct cccgggcccg gttgatttcc tggaaggagg tgaagggaag atagatttcc
      361 ccccgaacct cgacctcccg ccgttcgggg atggtcagcg gcaaggttcg gatcgtccgg
      421 gcgttggcgg tcacgtcgtc gccttggatt ccgtctcccc gggtcacggc ttggatcaag
      481 cgcccgttcc gataaaggag agagacgctc aggccgtcga ttttcagctc ggcgacgtag
      541 gagatcgtcc ggccggggag aaggcggagg aggcggcttt cgaattcctc gaattcctcg
      601 acggaataca cgttgtccaa gctgagcatc ggggtgcggt gccggacgga gggaaagcct
      661 tcgaccggtt gtccgccgac ccgctgggtc ggcgattccg ggacgatgag ttcgggatgc
      721 gcttcttcca ggcgccggag ctccctcatc atggcatcga attccgcgtc ggaaatctgg
      781 ggatcgttgt cctggtagta cttcttttcg tgataagcga ttttcttcct gaggcggtcg
      841 atcgccgcgg cggcggagcg gtccatgccc ttcattttag cgaaatcgcc cctctaaaga
      901 aaatccccct cggctccctc cgttcctttc ggatcgggga aggcgagggg gatggccgga
      961 ccgtccgtcg gcccggagct tacttgccga cgcgcttttt cgccgacgcc acgagggcgt
     1021 cgaacttgct cgatccggct ttcatctggg cgacgaggtt gttgaccgca accgtttcct
     1081 tgtcgatcaa ttccttgagc ttgcggaatt cccgccgctc gtcgggagtc aggtcctcct
     1141 cgcttttatc gccgtacttg gagttgatgg catcggtcca ttccttgatg agggcctggc
     1201 tctcgtcgat catcttgatc cggtcgttcc attccttgtc ctggccgaag aacaaggaac
     1261 gggcgatgtc ggtgcactgc ttcgaccgtt tcgtgtcaag aaagctggcg tcgagaagaa
     1321 aagcgacggc atcgttgacc agggccgggt tcgttttggc ctcgttggcc agcatgatcc
     1381 cgatgttata agccatggat cccgtcttgt tcttcgcgta catgtccttg aacatttgaa
     1441 gggcttcggc ttccttgccc tgcttgttga ggatctgggc cagaatcgtc atgctgtcgg
     1501 cgtccaggcc cgttccggcc aggtagcgga agatgggctc ggcctgggca tatttctgcg
     1561 catcgtaata gccctttccg attttcttga gctcgccgag aacggccggg gacttgaggg
     1621 tcgtataagc atggttgaag gagtcgatgg cccccttgat gtcgccggct ttttcctggg
     1681 ccttggccgt caggacgtac gcctggccga tcgtctgagt ccacatcttg gcgttggccg
     1741 cttccccctc tttccccttg gccgcattgg cgtgttgaat ggcctgctgg gcggccgctt
     1801 tggccttgtc gaactggttc gttttgagat ggatatcggc cacgatctgg tacatggcgc
     1861 ccttcatctg atcgtcgatc ttgccgctgg cggccgcctt ttccgccaag ccgatcgttt
     1921 cggcgttgta ctgcttggac tggtattgga tgatg
//
LOCUS       DAOOXH010000205         1911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_77285_length_1911_cov_6.575431, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000205 DAOOXH010000000
VERSION     DAOOXH010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77285_length_1911_cov_6.575431"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..1726
                     /locus_tag="PKV64_12090"
     CDS             221..1726
                     /locus_tag="PKV64_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17858.1"
                     /translation="MTKGSGTEEKNARFPGRTAAAGVIVFLLAAVPIVLTPIPPTTDL
                     PQHLNQVLLAIAHAGNGPGPYSVLWGAPNTLVYGFIFLLERVISLEYLGRAVLLSMIA
                     AWIFAIFFTAGRRTRPLEGAVLAALFLFNVSFYWGFLNFLAGFPVFIAWFFLTVRKEE
                     NPPAWWYAATVVASLFLYFGHVLWFVAGAAWFGLISLLRRLPLRSLAFRAATLIPACL
                     LSFFWYRKFAAFQAASELDLAAEWINRPFEKLFTMWFRGAAFGGGTGGLETILFFFLL
                     AWAGLSLATNRKDWRRRVDGDLAAAAGLFFAIAFFAPDTVRNTIYFSGRWAAVSFILA
                     VLSLPAPRIKPALRRTAAWGAVLAFYLVTTIHWAGYERTELTGLKESLARIPRASRVL
                     GLDYVKMSRYFKHRPFLQLYAYAHVFRDAELGLSFARHSTGLVAFRRTRPNPWTPKLD
                     WYSERVKESDLAYFDYVLVNGKPYDHNRRLPRRRLAPVTTAGRWRLYRVEK"
BASE COUNT          295 a          605 c          588 g          423 t
ORIGIN      
        1 catggggggg tcgatccgga aaaaagatag cacagggccc gggcgaagac aagggcggat
       61 accccccttt cccgtcccgg tccccgaaag ccgcgggggc ggagatcgcc gttcgcgtcc
      121 cgaacccgtc atttccttga catcggcgcg agacggggaa taagatggcc ggcatatgcg
      181 gcgtccggcg tcggaaatca aaaccggtgg tagaccggga gtgacgaagg gatccggcac
      241 ggaggaaaaa aacgcccgat ttccgggccg cacagccgcc gccggggtga tcgtctttct
      301 cctggccgcg gttcccatcg ttctcacgcc gattccgccg acgaccgacc tcccccagca
      361 tctcaatcag gttctcttgg cgatcgccca cgccgggaac gggcccggcc cgtactccgt
      421 tctttggggc gcgcccaaca ccctggtcta cggcttcatt tttctcttgg aacgcgttat
      481 ttcccttgaa tatctcggcc gggcggtcct cttgtccatg atcgcggcct ggattttcgc
      541 gatctttttc acggccggca ggcggacgcg tccgctggag ggcgccgttc tcgccgctct
      601 ctttcttttc aatgtctcct tctactgggg ctttctcaat ttcctcgccg gttttccggt
      661 tttcatcgcc tggttttttt tgacggtccg gaaggaggag aatcctccgg cctggtggta
      721 tgccgcgacg gtggtcgcat ccttatttct gtatttcggg catgtcctct ggtttgtcgc
      781 cggggcggcc tggttcgggt tgatcagcct cctccgccgg ctgcccctcc gatccctcgc
      841 cttccgggcc gcgacgttga ttcccgcttg ccttctttcc tttttctggt accgaaagtt
      901 cgccgcattc caggcggcct ccgagctgga cctggccgcg gaatggatca accggccttt
      961 cgagaagctg ttcaccatgt ggttccgggg cgcggccttc ggcggcggga cgggcgggct
     1021 tgagacgatt ctcttctttt tccttctcgc ttgggccggc ctttcgctgg ccaccaaccg
     1081 caaggattgg cggcggcgag tcgacggcga tctggcggcc gcggcgggct tgtttttcgc
     1141 gatcgccttc ttcgctcccg acactgtccg caacaccatt tatttctcgg gacgatgggc
     1201 ggcggtgagc ttcattttgg ccgtcctgtc gctgcccgcc ccgcggatca agccggccct
     1261 ccgccggacg gcggcctggg gggccgtgct cgccttttat ctcgtgacga ccatccattg
     1321 ggccggatat gagaggacgg agctgacggg cttgaaggaa agcctggccc gcattccccg
     1381 ggcctcgcgc gttctgggcc tggactacgt gaagatgagc cgctatttca agcaccgtcc
     1441 ctttctccag ctttatgcct acgcccacgt gttccgcgac gcggaattgg gtctttcctt
     1501 cgcgcggcat tccacgggcc tggtcgcttt ccggcggacg aggccgaatc cctggacgcc
     1561 caagttggat tggtactccg agcgggtcaa ggaatccgac ctggcgtact tcgattacgt
     1621 cctggtcaac gggaagccct atgaccacaa ccgccgcctg ccccggcggc ggctggcgcc
     1681 ggtgacgacg gccggacgat ggcgcctcta ccgggtcgaa aaataatccg gccgggcgtg
     1741 aaaaaaatcc tttcccaaat cccggttcaa ttgtataatg gccgcagcct ccggtccttg
     1801 ggaaggggcc ggcggagttt ggaaagacgg ggaattagga agcggagcaa aagaacggat
     1861 gctttccaat aaactccggg aaaggctgcc ggaagggttc cggttccagg a
//
LOCUS       DAOOXH010000206         1897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_78221_length_1897_cov_4.687296, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000206 DAOOXH010000000
VERSION     DAOOXH010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78221_length_1897_cov_4.687296"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(458..>1897)
                     /locus_tag="PKV64_12095"
     CDS             complement(458..>1897)
                     /locus_tag="PKV64_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPN17859.1"
                     /translation="VFYHKLGDPQEKDILVYQDTKNPLLYFSVSVSEDEKYAFLTVSA
                     GTSGTELYWKSLEAPGTEFSLLIPGFEADSYVLDNIGDKFLVQTNLHAPNARIVLVDP
                     REPAENKWTTIIPEKLEVLSGAGMAGGKLFCSYLKDASTKVFQYEPDGALIREVALPG
                     LGSAGGFGGWKDDRVVFYTFTSYNYPTTIFKFDIASGASEVFRKPEVKFNPEDFTVKQ
                     VFYASKDGTKVPMFIVHKKGLALDGVNPCFLTAYGGFNISLQPSFSPAHIALLENGVV
                     FAEPNLRGGGEYGETWHKAGMLGNKQNVFDDFIAAAEYLIAENYTSPERLGIEGGSNG
                     GLLVGACMTQRPDLFRVALPAVGVMDMLRFHKFTVGWGWIVEYGSSDNEKDFAYLYKY
                     SPLHNLKAGVRYPATLVTTADHDDRVVPAHSFKFIAALQKCQAGPEPVLIRIETRSGH
                     GSSNLTKGIEITADTFSFFLFHTGAKVAF"
BASE COUNT          352 a          579 c          602 g          364 t
ORIGIN      
        1 aggccgtcgg gtcccggccg ttggtcgtac accgtgatcc gggccaagcc gatgctcccg
       61 acgatcccct cggcctttcc cgccgggccg gagtcccggc aggccgcgag gagaaaagcc
      121 gatgccaaaa ccgctccgat cctcagagcc gcatgtttca tgaaaacctc ccgtccgggg
      181 aatcataatc ggaggaggcc gccccgtcaa cggcggcggc ccaagcgccc tttgggggga
      241 ttcggccgag agcccgccgg ggcttccgga ccccctcgcg ggagtcctgc tctgatttca
      301 gaggcggttc ctcccgcccg gtcgaacggc tcgccgcctc cttgccggag cgctcgtttt
      361 gtttcgtccc tgatgcccca tccgagcccg ggagccgaaa aaaaaagccc cctccccgaa
      421 ggggaggggg cgggacgggc ttgtcccgaa agcgcggtca gaaagcgacc ttggctcccg
      481 tgtggaacag gaagaaggaa aacgtatcgg ccgtgatctc gatgcccttc gtcaggttgg
      541 acgatccgtg gccgcttcgg gtttcgatcc ggatcagtac gggctccggg ccggcctggc
      601 atttctgcaa ggcggcgatg aacttaaacg aatgggccgg gacgacgcgg tcgtcgtggt
      661 cggccgtggt gacgagcgtc gccgggtagc ggacgccggc cttgaggtta tggagaggcg
      721 aatacttgta gaggtaggcg aaatccttct cgttgtcgct cgatccgtat tcgacgatcc
      781 agccccaacc gacggtgaac ttgtggaatc ggagcatgtc catgactccg acggcgggca
      841 gggcgaccct gaagagatcg ggtctctggg tcatgcaggc gccgaccagg agaccgccgt
      901 tgctcccgcc ctcgatgccg agccgctccg gcgatgtgta attctcagcg atcaagtact
      961 cggccgcggc gatgaagtcg tcgaaaacgt tttgcttgtt gccgagcatc ccggccttgt
     1021 gccacgtttc gccgtattcc cccccgccgc gcaggttggg ctcggcgaag acgacgccgt
     1081 tctcgagcaa ggcgatatgg gccggagaga aggaaggctg gaggctgatg ttgaagccgc
     1141 cgtaggccgt cagaaagcag ggattgacgc cgtccaaggc caggcccttc ttatggacga
     1201 tgaacatcgg caccttcgtc ccgtccttgc tggcgtagaa gacctgcttg accgtgaaat
     1261 cctcgggatt gaatttcacc tcgggcttgc ggaagacctc cgaagctccc gaggcgatgt
     1321 cgaacttgaa aatcgtcgtc ggatagttgt aggaggtgaa cgtgtaaaaa acgacccggt
     1381 cgtccttcca gccgccgaat ccgcccgctg aaccgaggcc cggaagggcg acttcccgaa
     1441 tcagggctcc gtccggctca tactggaaga ccttcgtgct ggcatccttt aaataggagc
     1501 agaaaagctt ccccccggcc atcccggccc cgctcaacac ctcgagcttt tccgggatga
     1561 tcgtcgtcca cttgttctcg gccggctccc gcgggtcgac gaggacgatc ctggcgttgg
     1621 gcgcatggag gttggtctgg acgagaaact tgtccccgat gttgtccagg acatagctgt
     1681 ccgcttcgaa cccggggatg aggagggaaa attccgtccc gggcgcttcg agggatttcc
     1741 agtacaactc cgttcccgac gttccggcgc tgaccgtcag aaaggcgtat ttctcgtcct
     1801 cgctgacgct gaccgagaag tagagaagcg gattcttcgt atcctggtag acaaggatgt
     1861 ccttttcctg gggatcgccg agcttgtgat agaacac
//
LOCUS       DAOOXH010000207         1879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_79377_length_1879_cov_5.932018, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000207 DAOOXH010000000
VERSION     DAOOXH010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79377_length_1879_cov_5.932018"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1500
                     /locus_tag="PKV64_12100"
     CDS             <1..1500
                     /locus_tag="PKV64_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17860.1"
                     /translation="IAALTETYGQFGSGLPGQRLEGAYDNSYLVADPREAWILETAGI
                     RWAAKRIEGGTASISNTLSLGGSLDLSSADLAAHAREKGWWTGSSEAAFSFEQAYSAE
                     GRDQEIARGRAQVRANCSLGLLREKSGSIDESWMKRIARDRSTDPSLDLDATASSCVA
                     SLPADGGGLPVFWWCASVPSSGIFVPFFVHGTELPAFLSAAGTAGKRVVAPETAPTDR
                     YSPDSYWWVFRDLTDLVNLDRPGRLAAVRKEFDALEQSFAAALPPVLKSATELRKAGK
                     TVEAARVLDDFSAACVERAAAAARALRDSWKPAGSDKSAAPEEAGVYIANFGAFADAE
                     WNVSARDGRLFLEIPGQGALELRPPDAEGFRALAASPQAGVSFSRRPEFGVTAMIFRR
                     GAMSFELPRKGIVLPPEIPLEELRKFLGEYHGDELDETLEIVIKNNSLALKISGQKTY
                     ELRPPDAEGKRFFRVAPLVYLVFKESETGGVESFTYHQGPSSLTYEKIK"
BASE COUNT          333 a          580 c          598 g          368 t
ORIGIN      
        1 atcgcggcct tgacggaaac gtacgggcag ttcggctccg gccttcccgg ccaaaggctg
       61 gaaggcgcgt atgacaattc ttatctcgtc gccgatccga gggaagcctg gattttagaa
      121 acggccggaa tccgctgggc ggcgaagagg atcgagggcg gaacggcctc gatctccaac
      181 accctgagcc tgggcggttc ccttgacttg tcgtcggccg accttgcggc ccatgcccgg
      241 gagaaaggct ggtggacggg aagctccgaa gccgcgttca gtttcgaaca ggcatattcg
      301 gccgagggac gggatcaaga aatcgcccgg gggagagccc aagtccgggc gaactgttcc
      361 cttgggcttc tccgggaaaa atccggatcg atcgacgaat cttggatgaa gcggatcgcc
      421 cgcgaccggt ccaccgaccc gagcctggac ctggatgcga cggccagctc ctgtgtcgcc
      481 tcgcttcccg ccgacggcgg cggactcccc gttttctggt ggtgcgcgtc cgtcccctca
      541 agcgggattt tcgtcccgtt ctttgttcat ggaacggagc ttccggcctt cctctcggcc
      601 gccggaacgg cggggaagcg cgtcgtcgcg ccggagacgg ccccgaccga ccggtattcg
      661 cccgattcct actggtgggt cttccgcgac ttgaccgacc tcgtcaatct cgaccggccg
      721 ggccggctcg cggccgtccg aaaggaattc gacgctttgg aacaatcctt cgccgccgcc
      781 ctcccgcctg tcctcaagtc ggccacggaa ctacggaagg ccggaaagac cgtggaagcg
      841 gcccgggtgt tggacgattt ctcggccgcc tgcgtcgaaa gagccgcggc cgcggcccgg
      901 gcgcttcggg attcctggaa accggccggt tcagacaaat cggcggcgcc ggaagaggcg
      961 ggcgtctata tcgccaattt cggggctttc gcggacgcgg aatggaacgt ctcggcccgg
     1021 gacggccgcc tgttcctgga gatcccggga cagggcgccc ttgagctccg tccgccggac
     1081 gccgagggct tccgggccct cgccgcctct ccccaagccg gcgtttcctt cagccggcgg
     1141 cccgaattcg gcgtcacggc catgatcttc cgccgggggg cgatgtcctt cgagctgccg
     1201 cgaaagggaa tcgtcctgcc gccggagatt cccctggaag agctccggaa atttcttggg
     1261 gagtaccacg gcgacgagct cgacgaaacc ctggagattg tcatcaagaa caacagcctg
     1321 gcgttgaaaa tctccggaca gaaaacctac gagctgcgtc ccccggatgc ggagggaaaa
     1381 cgatttttcc gcgtggctcc tttggtttac ctggttttca aagaatcgga aacgggaggc
     1441 gtcgagtctt ttacttacca ccaaggcccc tcgtctttaa cttatgagaa aatcaaatga
     1501 ttcagggaag ccgggactcc gggaattcat tgatttcaat caagaacgct tcagagggag
     1561 ggatttcccc gcttttcgcg gctgctccct ccggcggcct tggtggattc gtttttcgat
     1621 gagaccgacg atcgtctttc agccggccgg aggaaacgcc gccgggacct ctttttatgg
     1681 atcgtttttt ttatcgtccc tcccatgagg ggggcgatga tcgggccgct tccgatgagg
     1741 gaagaagacg gggcccgggg cgtctaataa gaagggagag cggtttctgt tttccggccg
     1801 tcccggccgg ctcccgacca tttcaatttc acgaggagga ggacccgcat gaagcgcgtc
     1861 ttgacgcttt cgctcgcgg
//
LOCUS       DAOOXH010000208         1858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_80744_length_1858_cov_6.997227, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000208 DAOOXH010000000
VERSION     DAOOXH010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80744_length_1858_cov_6.997227"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="PKV64_12105"
     CDS             <1..1307
                     /locus_tag="PKV64_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN17861.1"
                     /translation="ETAGFFHPARPFYRFSVLFFVALLSFGSYFAYDIVSAIAPTLVE
                     DLNAARGTVGAFFTAYSVAAILAVLLGGMLIDKLGTRKSSLIFSLLVFIGALIVWKAK
                     TIPLLLAGRFIFGAGSEPLIVAQSAILARWFKDKELAFSFGLSLTFSRLGSLFAFNTG
                     ELFTSYYGTYRAALFVAMAACGISLAANLVYIVMDRRGERVLRLRDESQGEKIVFSAL
                     KSFKPSFWYVTFLCLTFYAAIFPFTNLSTDFFVDKWGIARVAEGTGGFLARVFENLLH
                     LFTTAGGISSIIIFASMILAPFAGKLVDRIGRRATLMIFGSLILIPSHLAMGLTRIYP
                     VYPMLALGAAFVLVPAALWPSIPLIVKSERVGTAFGTMTAIQNVGMAVFPLLNGLLRD
                     WTHSYAASMVMFACLGVVGLVFALLLRRADQREGGVLERAGR"
     gene            1304..>1858
                     /locus_tag="PKV64_12110"
     CDS             1304..>1858
                     /locus_tag="PKV64_12110"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPN17862.1"
                     /translation="MIETVVSDLGNVLLRFDNSVFYRAIARFSGRPEKEIEAVVADNL
                     DLLVLFEKGALSPYDFYRNSRDLLEWTGGYEEFFSAYCEGVFTPVPAVLGLFRKLKSR
                     YRTVLLSNTDVIRWTHVKTKFPDLLIFDEYILSFDVGFRKPQLEIYLEAIRAGGAAPG
                     RTAFIDDLPDNVAGAERAGMKGILC"
BASE COUNT          266 a          624 c          540 g          428 t
ORIGIN      
        1 cggagaccgc cggatttttt caccccgcgc gtccgttcta ccgcttttcc gtcctctttt
       61 tcgtcgccct cctttccttc gggagctatt tcgcctacga tatcgtctcg gccatcgccc
      121 cgacgctggt cgaggatctt aacgccgccc gggggacggt cggggccttt ttcacggcct
      181 acagcgtggc cgccatcctg gccgttcttc tcggcggaat gttgatcgat aagctcggaa
      241 cccgcaaatc ctccctgatc ttctccctcc tcgttttcat cggcgcgctc atcgtctgga
      301 aagcgaaaac cattccgctc cttctcgccg gacggttcat cttcggcgcc ggctcggaac
      361 cgctcatcgt cgcccagtcg gcgatcctgg cccgctggtt caaggacaag gagttggcct
      421 tttccttcgg cctgtccctg accttcagcc gcttggggtc cctgttcgct ttcaacaccg
      481 gcgagctctt caccagctac tacgggacgt accgggcggc cctcttcgtg gccatggccg
      541 cctgcggcat ctcgctggcg gccaacctgg tctacatcgt catggaccgc cggggcgagc
      601 gcgtcctccg cctccgggat gaaagccagg gggagaagat cgtgttctcc gccctcaagt
      661 cgttcaaacc gtcgttctgg tacgtgacgt tcctctgtct gaccttctac gcggcgattt
      721 tccccttcac caacctgtcg acggatttct tcgtcgacaa gtgggggatc gcccgggtgg
      781 ccgaggggac gggcggattc ctggcccggg tgttcgagaa cctgctccat ctcttcacca
      841 cggccggggg catttcctcg atcatcatct tcgcctccat gatcctggcg ccgttcgccg
      901 gcaagctcgt cgaccggatc ggccggcggg cgaccctgat gatcttcggg tcgctgatcc
      961 tcatccccag ccacctggcc atgggcttga cccggattta tccggtctat ccgatgctgg
     1021 ccctgggagc ggctttcgtc ctcgtccccg ccgccctgtg gccgtcgatt ccgctgatcg
     1081 tcaagagcga gcgggtcggg acggcgttcg ggacgatgac ggccatccaa aacgtcggga
     1141 tggccgtctt tcccctgctc aacggcttgc tccgggactg gacgcattcc tatgccgcga
     1201 gcatggtgat gttcgcctgc ctcggggtcg tcggcctcgt cttcgccctc cttttgcggc
     1261 gggccgatca acgggaggga ggggtgctgg agcgggcggg ccggtgatcg aaaccgtcgt
     1321 ttccgatctg ggcaacgtcc tcctccggtt cgacaactcc gttttttacc gggcgatcgc
     1381 ccggttttcc ggccggccgg agaaagaaat cgaagcggtc gtcgccgaca atctcgatct
     1441 tctcgttctc tttgaaaaag gcgctctttc gccgtatgat ttctaccgga attcccgcga
     1501 cctgctggaa tggacgggcg gatacgagga atttttctcg gcctactgcg agggcgtgtt
     1561 cacgccggtc ccggccgtcc tcggcctctt ccggaagctg aaatcccgct atcggacggt
     1621 ccttctttcc aacaccgacg tcatccgctg gacgcacgtc aagacgaaat tccccgacct
     1681 tttgatcttc gacgagtaca tcctctcgtt cgatgtcgga ttccggaagc cccagctcga
     1741 gatttacctc gaggcgattc gcgccggcgg ggccgccccc gggcggacgg cgttcatcga
     1801 cgaccttccg gacaatgtgg ccggcgccga gcgcgcggga atgaagggca ttttatgt
//
LOCUS       DAOOXH010000209         1767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_87316_length_1767_cov_3.801986, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000209 DAOOXH010000000
VERSION     DAOOXH010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87316_length_1767_cov_3.801986"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="PKV64_12115"
     CDS             complement(<1..1117)
                     /locus_tag="PKV64_12115"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPN17863.1"
                     /translation="MKKTPARAFAAILITLPLLAADQTAAKDPGKKTEGIGAGTFAGL
                     AWRSIGPAHTSGRIADFAVNPGNPKQYFVGVASGHIWKTDNAGITWTPVFDNYGAYAI
                     GCLAMDPANPNVVWAGTGENNHQRALGYGDGVYKTVDGGKSWTHMGLKNSRQIGVILI
                     HPKNPDTVYVAAEGSAWGPGGDRGLYKTADGGKTWAKVLSISENTGVNSAVMDPRDPD
                     VLLVTSEQRRRHVFTKIGGGPETAFYKTTDGGKTWRKITAGLPSAPMGGIGLALSPVD
                     PDVVYAIIEAAGDAGGFFRSSDRGESWTKMSAYHSSGQYYNEITCDPKDVDTVYSMET
                     VSRVTRDGGRTWTPLGLDARHVDDHALWIDPRDTAHFL"
BASE COUNT          335 a          505 c          552 g          375 t
ORIGIN      
        1 tgaggaagtg ggcggtgtcc cgcgggtcga tccagagggc gtggtcgtcg acgtggcggg
       61 cgtccaggcc caggggcgtc caggtgcgcc cgccgtcgcg ggtgacccgg gagacggtct
      121 ccatggaata gaccgtgtcg acgtccttcg ggtcgcacgt gatctcgttg tagtactgcc
      181 ccgaggaatg ataggcgctc attttcgtcc aggactcgcc gcggtcggac gaacggaaaa
      241 agccgcccgc gtccccggcc gcttcgatga tggcatagac gacgtcggga tcgacggggg
      301 aaagggccag cccgatccct cccatggggg cggagggcag gcccgccgtg attttccgcc
      361 aggtcttgcc gccgtccgtc gttttataaa acgccgtttc cggtccgccg ccgatcttgg
      421 tgaaaacgtg ccggcggcgc tgttccgagg tgacgaggag aacgtcggga tcgcgcggat
      481 ccatgaccgc ggagttgacg ccggtgttct cgctgatcga cagcaccttg gcccaggtct
      541 tcccgccgtc ggccgtcttg tagaggccgc ggtcgccgcc gggaccccag gccgagcctt
      601 cggccgcgac gtagaccgtg tcgggattct tgggatggat gaggatgacc ccgatctgcc
      661 gggagttctt caggcccatg tgcgtccacg acttgccgcc gtcgacggtc ttgtatacgc
      721 cgtcgccgta gccgagggcc cgctggtggt tgttttcgcc ggtcccggcc caaacgacgt
      781 tgggattggc cgggtccatg gccagacagc cgatggcgta cgcgccgtaa ttgtcgaaca
      841 ccggcgtcca ggtgatgccg gcgttgtcgg tcttccagat gtggccgctg gccacgccga
      901 cgaagtattg cttgggattt ccgggattga cggcgaaatc ggcgatgcgg ccggaggtat
      961 gggccgggcc gatcgaacgc caagccaggc cggcgaacgt tccggcgccg attccctccg
     1021 ttttcttccc cgggtccttc gcggcggttt gatcggccgc gaggaggggg agagtgatca
     1081 ggattgcggc gaaagcgcgg gcgggcgtct ttttcatccg agcctccttg aattgatggg
     1141 atggaaggca cgaatcgtaa cccggggccg ggctcctgtc aatgaaacga atcgcgccat
     1201 ctaaaatctt accctcctct ctttgcctga ggaggccgga ggatggatct tcacagccgg
     1261 agggaactca tggaatccat gtccaaacga tatcgtcaag cctcaagagc ttcaaatatg
     1321 cctcctgcca tgatcgcgac atcggatttc cccgaagccg gccctgcaag aaatacgata
     1381 atgcccagat cgtgcagaaa aaatggttat gatctctcaa cgatcttcgg ccgcgataag
     1441 gtttttggag agcaagacaa tgccctcttg agtaagattt taatatagct tgacaggcct
     1501 cgcccgatca aatattctca tttccgaggg ggtggataga atttcgcgat aagacaaagc
     1561 tcgcggcttc gaccggttga aagacgaaat tcctatcctt tggttgcgtt atttcggcca
     1621 agaataatta atgctcaatt agatttattt tcatgaagaa gcgccggaag aaacagctta
     1681 tctcccgcgg cttcgccgca tcgttgcctt caattttaga aatcgaatcc tgcacccggt
     1741 gcaacatcca tcctccgtgt gtcatgt
//
LOCUS       DAOOXH010000210         1717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_91245_length_1717_cov_6.696751, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000210 DAOOXH010000000
VERSION     DAOOXH010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91245_length_1717_cov_6.696751"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PKV64_12120"
     CDS             <1..1011
                     /locus_tag="PKV64_12120"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPN17864.1"
                     /translation="GRTDAGTVETVGGLTLDDAKAFYREHFVRGNITIGLAGGYPEEF
                     PEEVRRDFEKLPEGFTPRLALPPAPTPKGLQFVLAEKDTPGTAVSMGFPVSITKSDKD
                     FFALWVAGAHFGEHRQHVSLLFQKIREERGQNYGDYAYIEHFVEGRDKFPAPGHARRQ
                     QYFSIWIRPLPNSNRHFVIRQTLRELKRLVDEGLSEERFELVRTYLLNYTKLYAQTLD
                     ERLGWRMDSRFYGYEDFLAEAARILPRLTRNDVNAAVKKYLQASDIRIAVITPDAGQL
                     RDDLVSNKPSPIRYANPNMPAGVINEDTVIQIYSLDVQPENVRIVPAGEFFRRPGIPA
                     GR"
     gene            1008..1700
                     /locus_tag="PKV64_12125"
     CDS             1008..1700
                     /locus_tag="PKV64_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17865.1"
                     /translation="MSRGRPAGKGADTRRGRASPPTSGGEADWTPAERRLLASLGSPE
                     KIQFFLNRLAYDPAPGTASPRRVLRERKANCFEGALLAAAVFRHHGRPPLLVDMRSWN
                     DDDHVLAVFRHNGAWGCVAKSNYTVLRFREPVYRTIRELMMSFFDIFFNAIGQKTLRQ
                     YSVPFDLGRFESRGWMTTPDDISWIGDALDRARHYQVLTPAQIRELHRADPVLVKAGL
                     MGADPSGLFKPK"
BASE COUNT          310 a          599 c          507 g          301 t
ORIGIN      
        1 ggccggaccg acgccgggac ggtcgagacc gtcggcgggc tgactctcga cgacgcgaaa
       61 gccttctacc gggagcactt cgtccggggg aacatcacga tcggcctggc cggaggatac
      121 cccgaggaat tcccggagga ggtccggcgg gactttgaga agctcccgga gggattcact
      181 ccccggctgg ctcttccgcc ggctcccaca cccaaaggtc ttcaattcgt cctggccgaa
      241 aaggacacgc ccgggacggc cgtctccatg ggtttccccg tctcgatcac gaaatccgac
      301 aaggatttct tcgccttatg ggtcgccgga gcccacttcg gcgagcatcg ccagcacgtc
      361 tccctcctgt tccagaaaat ccgtgaagag cgcggccaaa actacgggga ttacgcgtat
      421 atcgaacact tcgtcgaggg ccgcgacaag ttcccggccc ccggccacgc ccgccggcag
      481 cagtactttt cgatctggat ccggcctctg cccaactcca accgccattt cgtcatccgc
      541 cagacgctcc gcgagctgaa gcgtctggtc gacgaagggc tctcggaaga gcggttcgag
      601 ctcgtccgga cctaccttct caattacacc aagctttacg cccaaaccct ggacgaacgg
      661 ctcggctggc ggatggattc ccgtttctac ggatacgagg actttctggc tgaagccgcg
      721 cgaatcctcc cccggctgac gcggaacgac gtgaacgccg ccgtgaaaaa gtatctccag
      781 gcgtccgaca tccgtatcgc cgtgatcact ccggacgccg gacaattgcg ggacgatctt
      841 gtctccaaca agccgtcgcc gatccgctat gccaatccga acatgccggc cggcgtgatc
      901 aacgaggaca cggtcatcca gatctactcc ctggacgttc aaccggaaaa cgtccggatc
      961 gttcccgccg gggaattttt ccgccggcct ggaatccccg ccggcagatg agccgaggcc
     1021 gcccggcggg aaaaggcgcg gacacgcgcc gaggccgggc ttctccgccg acaagcggcg
     1081 gggaggccga ctggacgccg gctgagcgaa ggctcctggc ttccctcggc tcgccggaga
     1141 aaatccagtt ttttctcaac cgcctcgcct acgacccggc gccggggacc gcgtcccccc
     1201 ggcgcgttct ccgggagcgg aaagccaact gcttcgaggg ggcgctcttg gccgccgccg
     1261 tcttccggca tcacggccgg ccgccgctct tggtcgacat gcgctcttgg aacgacgacg
     1321 accatgtcct ggccgtcttc cggcacaacg gagcctgggg ctgcgtcgcc aaatcgaatt
     1381 acaccgttct ccgcttccgg gagcccgtct accggacgat ccgggagctg atgatgtcct
     1441 ttttcgacat ctttttcaac gcgatcggac agaagacgct ccgccagtat tccgtccctt
     1501 tcgacctcgg gcggttcgaa tcacgcggct ggatgaccac gcccgacgac atcagctgga
     1561 tcggcgacgc tctcgaccgg gcccgccatt accaggttct cacccccgcc caaatccggg
     1621 agctccaccg ggccgatccg gtcctggtca aagccgggct gatgggcgcg gatccctcgg
     1681 gcctgtttaa gccgaaataa tcccggcggc ggtcgtg
//
LOCUS       DAOOXH010000211         1683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_94082_length_1683_cov_5.262899, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000211 DAOOXH010000000
VERSION     DAOOXH010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94082_length_1683_cov_5.262899"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1683)
                     /locus_tag="PKV64_12130"
     CDS             complement(<1..>1683)
                     /locus_tag="PKV64_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17866.1"
                     /translation="GPAAADLYVGVDFFNAAAARAAAEARRLPLVWAGPLKYVRVPER
                     LVLLHAFEKKTPFPPAWEKLRRTVRCFGPEQEALARKRFGAEIESAFLRTQEIAGKCR
                     FDFRDVVPPLPADLFPVTLRDEVMARLRRPGDLSWAERERALRELDVVERSGFGPYFL
                     IVHDIVSFARKNGILHNLKGSGASSFLAWLLGISHVNPVAFDLYFERFLNSGRADPPD
                     FDLDFDSRRRDRVLAYVLERYGRGRTGAAFVCALKSYGARSALYETARAFGLPPAEAR
                     ALSRRAPYYETPEYMKKNAAPAGCLDIWKAAAELAGVHHEVSLHVGGVIFTPAPADRH
                     LPLEHSAKGVLMSHYDRDAVEDLKLIKLDLLSVRGLAAISETKTRLGLGDLPPGDPAA
                     YGLLKKAGTIGCFQVESPAMMNLLRRMKPGDIQDLTQALALIRPGPTECGMKESVLRV
                     REEGGGTYRDPFLAGILPETGGVLLYEEQIMQIAERTAGMPPEEGDLLRRSLKRRGNG
                     AAPDPLREKFFREARTRGYTGAEVEKLWGMMEKFSSYAFNKAHSASYAFMAYQ"
BASE COUNT          272 a          550 c          605 g          256 t
ORIGIN      
        1 ctggtaggcc atgaaggcgt aggacgcgct gtgagccttg ttgaaggcat aggacgaaaa
       61 cttctccatc atcccccaga gcttctccac ctcggccccc gtgtagccgc gggttcgggc
      121 ttcccggaaa aacttttccc ggagcggatc gggagccgcc ccgttccccc ggcgcttgag
      181 gctccgccga aggagatccc cctcctccgg cggcatcccc gccgtccgct cggcgatctg
      241 catgatctgt tcctcgtaga ggaggacgcc gcccgtttcg ggcaggatcc cggccaggaa
      301 aggatcgcgg tatgtccctc cgccctcctc ccggacgcgg aggacgctct ccttcatccc
      361 gcattcggtc ggcccgggcc ggatcagggc cagggcctgg gtcaggtcct ggatgtcgcc
      421 gggtttcatc cggcggagaa gattcatcat ggccgggctc tcgacctgga agcagccgat
      481 cgtcccggct ttcttgagga ggccgtaggc cgccgggtcg ccgggcggaa ggtcgccgag
      541 gccgaggcgg gttttcgtct cggaaatggc cgccaggccg cggaccgaca ggaggtcgag
      601 cttgatgagc ttaaggtcct cgaccgcgtc ccgatcgtag tgggacatga ggacgccctt
      661 ggccgaatgt tccagaggaa gatggcggtc ggccggggcg ggggtgaaga tgacgcctcc
      721 gacgtggagg gagacctcgt ggtgaacgcc ggccagctcg gcggccgcct tccagatgtc
      781 caggcatccg gccggagcgg cgtttttttt catgtactcc ggcgtttcat aatacggcgc
      841 cctccggctc aacgcccggg cctccgccgg cggcaggccg aacgcccggg cggtctcgta
      901 gagggccgac cgggcgccgt agcttttcaa ggcgcagacg aaggccgccc cggtccggcc
      961 ccggccgtag cgctcgagga cgtaggcgag gacccggtcg cggcggcggg agtcgaagtc
     1021 aagatcgaag tcgggcggat cggcccgtcc gctgttgagg aagcgctcga aatagaggtc
     1081 gaaggccacc gggttgacgt gggagatgcc cagcagccag gccaggaacg acgaggctcc
     1141 cgagcccttg aggttgtgga gaatgccgtt tttgcgggcg aaggaaacga tgtcgtggac
     1201 gatcaggaag tacgggccga agcccgagcg ctcgacgacg tccagctccc gcagggcccg
     1261 ctcgcgctcg gcccagctca gatcccccgg ccgccggagg cgggccatca cctcgtcgcg
     1321 gagggtgacg gggaaaaggt cggccgggag cggcgggacg acgtcccgga aatcgaaccg
     1381 gcatttcccg gcgatctcct gcgtccgaag aaaggcggac tcgatctcgg ccccgaatct
     1441 cttccgggcc agggcttcct gctccgggcc gaagcagcgg accgtccggc ggagcttctc
     1501 ccaggccggg ggaaacgggg tcttcttctc gaaggcgtgg aggaggacga gccgctcggg
     1561 gacccggacg tacttcagcg gaccggccca gacgagcgga aggcggcggg cctcggccgc
     1621 ggcccgggcc gcggccgcgt tgaagaaatc caccccgaca tagaggtcgg ccgccgccgg
     1681 gcc
//
LOCUS       DAOOXH010000212         1672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_95069_length_1672_cov_3.376005, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000212 DAOOXH010000000
VERSION     DAOOXH010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95069_length_1672_cov_3.376005"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="PKV64_12135"
     CDS             <1..1072
                     /locus_tag="PKV64_12135"
                     /inference="COORDINATES: protein motif:HMM:NF037222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HPN17867.1"
                     /translation="NKTDGGAFGFNTETGPGPQVPPLESLRKMFPADRLWPINDLWFY
                     HCCRGEFNDLSRYNEAMDKRLGPPTGLADYLRKAQFMNYEAMRAMYEAFVIRRPVATG
                     IIQWMYNSAWPKLWWQLFGYDLLPNGAFFGARKACEPLHILYDLSTREVFVTNDAGRP
                     SGPVRASVQVFGLDGARRSAREFPVALDADDKKSLGVVAPPAGAAGAWFLDLRLHDEA
                     GGLITDNFYALPLRDDVLDDAKTEWYVTPIKEFADLTGLAALPPAAIDCREERKTDGG
                     CLEIAATLKNTAAVPAFMIELMILNEKNGEAVTPVFWDDNYLTLLPDETRTVKVRINT
                     AGLDGAPAVLKIGGWNAVRAER"
     gene            1077..>1672
                     /locus_tag="PKV64_12140"
     CDS             1077..>1672
                     /locus_tag="PKV64_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPN17868.1"
                     /translation="MKRNPFLVVLVFLVFFVISLVTNVIGAINPDVGQSFGLSLTFVG
                     LLAFAFFVAYGVMSIPAGLLVERYREKPVMIAAFGLALSGALLISLWPSYPVFLASLF
                     LIGTGFAALQVVINPLLRVSGGEEHYAFNSVLAQLIFGGASFLSPFLYSYFVQNIGRS
                     GAEGKPLIGLMTKLVRPGLSWVKPDGGFVEYEQFGCID"
BASE COUNT          269 a          577 c          498 g          328 t
ORIGIN      
        1 caacaaaacg gacggcggcg ccttcggttt caacaccgag acggggcccg gaccccaggt
       61 tccgcctctc gaaagcctcc ggaaaatgtt cccggccgac cgcctctggc cgatcaacga
      121 cctgtggttt tatcattgct gccgcggcga gttcaacgac ctctcccggt acaacgaggc
      181 gatggacaag cggctgggac cgccgaccgg cctggccgat tacctccgca aggcccaatt
      241 catgaactac gaggccatgc gggcgatgta cgaggccttc gtcatccgcc gccccgtggc
      301 cacgggcatc atccagtgga tgtacaattc ggcctggccc aagctctggt ggcagctctt
      361 tggatacgat ctcctgccca acggggcctt tttcggcgcc cgcaaggcct gcgagccgct
      421 ccacatcctc tacgacctct cgacccggga agtattcgtc accaacgacg cgggtcggcc
      481 ttccgggccg gtccgggctt ccgttcaagt cttcgggctg gacggagcgc gccggtccgc
      541 ccgtgaattc ccggtcgccc tggacgccga cgacaagaaa agcctgggcg tcgtcgctcc
      601 gccggccggc gcggccgggg cctggttcct cgacctccgc ctccacgacg aggcgggcgg
      661 cttgatcacc gacaacttct acgccttgcc gctccgcgat gacgtcctcg acgacgcgaa
      721 gacggaatgg tacgtgacgc cgatcaagga attcgccgat ttgacgggcc tggccgccct
      781 tcccccggca gcgatcgact gccgggagga acggaagacc gacggcggat gcctggaaat
      841 cgcggcgacg ctgaaaaaca cggcggccgt cccggccttc atgatcgagc tcatgattct
      901 gaacgaaaaa aacggcgagg ccgtcacgcc tgtcttctgg gatgacaatt atctgacact
      961 ccttccggac gagacccgga cggtcaaggt ccggatcaat accgccgggc tggacggcgc
     1021 gccggccgtc ctcaagatcg gcggctggaa cgccgtccgg gcggaaaggt aagatcatga
     1081 agcgcaaccc cttcctcgtc gttctcgttt tcctcgtctt cttcgtcatt tcgctcgtca
     1141 ccaacgtcat cggggccatc aatcccgacg tcggccagag cttcggcctc agcctgacgt
     1201 tcgtcgggct cctggccttc gcctttttcg tcgcctacgg cgtcatgtcc atccccgccg
     1261 gcctgcttgt cgagcgctac cgggagaagc cggtcatgat cgcggccttc ggcctggccc
     1321 tgtccggggc cctgctcatc tcgctctggc cgagctatcc cgtgtttttg gcgtcgctct
     1381 tcctcatcgg gaccggtttc gccgccctcc aggtggtcat taatcccctg ctccgcgttt
     1441 ccggcggcga ggagcactac gccttcaact ccgtcctggc ccagctcatc ttcggcgggg
     1501 cttcgttcct gagcccgttc ctctattcct atttcgtcca gaacatcggc cggtccgggg
     1561 cggaaggcaa gccgctcatc gggctgatga ccaagcttgt ccggcccggc ttgtcgtggg
     1621 tcaagcccga cggtgggttc gtcgagtatg agcagttcgg gtgcattgat ga
//
LOCUS       DAOOXH010000213         1636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_98275_length_1636_cov_4.951929, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000213 DAOOXH010000000
VERSION     DAOOXH010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98275_length_1636_cov_4.951929"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="PKV64_12145"
     CDS             complement(<1..1208)
                     /locus_tag="PKV64_12145"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPN17869.1"
                     /translation="MGAPMSAGLFDVAVIGGGVVGCAVLRELSRYRLRLVLLERRSDL
                     AEGVSKGNSGVIHAGFNVRPGSLKARTNAAGLRAIYGLASALGVPHKKTGKLVVAFDE
                     DGRRHLEALRSQGERNGTPGLDIVDAAAIRNLEPEVRGAWALFSSWTGVISPYEFTIA
                     LAENAWRNGAEVRLESGVTAADCRPDRFILETPAGPVSARWVVNCAGLQADEVAGLAG
                     PADFRIRPFKGEYLVLDKAAGDLLRMPVYPVPPRDESSLGIHLTPTTEGPVLLGPSGE
                     TAEDKDDARSTRGIMDRLKAEAFRLVPALGRIPVIHAYAGSRPKLDGLATADGPADFL
                     VAESRRHPRWINLGGIESPGLTAAPALAEHVAGLIGAREDLRPREGFEPGLPGRPRFA
                     RLDDADRAARA"
BASE COUNT          276 a          551 c          567 g          242 t
ORIGIN      
        1 gcccgggcgg cccggtccgc gtcgtccagc cgggcgaagc gcggacggcc gggcaggccc
       61 ggctcgaatc cttccctcgg gcgcagatcc tccctcgccc cgatcaggcc ggcgacgtgt
      121 tcggccaggg cgggggcggc cgtcaggccg ggcgactcga tgccgccgag gttgatccag
      181 cggggatgcc ggcggctttc ggcgaccagg aaatcggccg gtccgtccgc cgtcgccaag
      241 ccgtcgagct tcggccgcga gccggcgtac gcgtggatga ccgggatccg gccgagggcc
      301 ggaaccagcc ggaaagcctc ggccttgagg cggtccatga tcccgcgggt cgagcgcgcg
      361 tcgtccttgt cctcggcggt ttcgccgctc ggacccagga ggacgggccc ctcggtcgtg
      421 ggggtcaggt ggatgccgag cgaggattcg tcccggggag gcacgggata gacgggcatc
      481 cggagaagat cgccggcggc cttgtccaaa acgaggtatt cgcccttgaa gggtcggatg
      541 cggaaatccg ccggcccggc caggccggcg acttcatcgg cttgaaggcc ggcgcagttg
      601 accacccagc gggcggaaac gggaccggcc ggcgtctcga ggataaaccg atccggccgg
      661 caatcggccg ccgtgacacc gctttcaagc cggacctcgg ccccgttcct ccaggcgttt
      721 tcggccaggg cgatggtgaa ttcatagggg gaaatgacgc cggtccaaga cgaaaagagg
      781 gcccaggcgc cccggacctc cggctcgaga ttccggatcg cggccgcatc gacgatgtcc
      841 aggccgggcg ttccgttccg ctccccctgg gaccggaggg cctcgagatg gcggcgtccg
      901 tcctcatcga aggcgacgac gagctttccg gttttcttgt ggggaacgcc gagggcggag
      961 gcgaggccgt agatcgcccg cagcccggcg gcgttcgtcc gggccttgag acttcccgga
     1021 cggacgttga agccggcgtg gatgacgccg ctgttgccct tgctcacgcc ctcggccagg
     1081 tcgctccgcc gttccaggag gacgaggcgc agccggtagc gggacagctc ccggagaacg
     1141 gcgcagccga cgactccgcc gccgatgacg gcgacgtcga acagtccggc ggacattggg
     1201 gcacccatgg ggaggaacga tcatatcaaa acggcttgcc ggccgtccac gggggaaagc
     1261 ggcccggaaa agaaatcacg ccccggctga tccccccggc gaaagaacgg aatctcccga
     1321 ggaacgagat gagaaagatc gcccctctac cgcggcggga tcgcggatcc gcggccgccg
     1381 ccccgggaag gcggatgttc cgaacgcatc ccgtccgaaa caggcggcga atccgaatcc
     1441 cgccggaaac gcaagaatgg aagacaagcg ccgcgttgca tcgtcccggc cgtttttggt
     1501 acattgcctc ccttcgatga gaaaaaagat cctgttctcg gcctcggtct tccatgccct
     1561 gaccgacgcg tccaccgtcg tcatcccgac gatttttccc atcctctaca accgcggttt
     1621 tctgatcacc cggtat
//
LOCUS       DAOOXH010000214         1630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_98801_length_1630_cov_7.128889, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000214 DAOOXH010000000
VERSION     DAOOXH010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98801_length_1630_cov_7.128889"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PKV64_12150"
     CDS             complement(<1..221)
                     /locus_tag="PKV64_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPN17870.1"
                     /translation="MLYALFKTLQASVPDFNLFRYITVRTALAGITAMALVLFLGPVF
                     IRGLRRFQVGQFVRSDGPASHLSKQGTPT"
     gene            complement(223..>1630)
                     /gene="murF"
                     /locus_tag="PKV64_12155"
     CDS             complement(223..>1630)
                     /gene="murF"
                     /locus_tag="PKV64_12155"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPN17871.1"
                     /translation="FGEEITMALLRASEIAAAAGGTVLQGDRGAVFSSFGLDSRTIER
                     GGLFFALVAGRDGHDFVPDAAARGAAGAVVSRGVPAADKSFVLIKTADTAAALQDLAR
                     SVLVRHPAKVVGVTGSVGKTTTKEFIAALLGTIFRVHKSEGNLNNHLGLSLSVLRLET
                     EHEVAVLEMGMSAAGEIRRLTGIAPPDVAVVTNVAPVHLAFFESLQAVAEAKAEILEG
                     LKPDGTAVLNADDPWSKTLADRAPGRVITFGFSQDAEVRASGMAYAGYDGLQFRLHYD
                     GAAREVTLPFLTGGQVENFLAALGVAAAFGLGWETLKPALAVLAPAAKRGNIIRLARG
                     ITVIDDTYNSNPRALDMALRSYINLPAGRWVAVLGDMLELGPEGDQYHEDAGRTAGRL
                     GWDIVAAVGPAARRIAAGARAEGLDAARTPEFASSEEAAAKLPALLKADDLVLVKGSR
                     GVRMERIVERLTALMKEN"
BASE COUNT          263 a          537 c          584 g          246 t
ORIGIN      
        1 atcgtcggcg tgccctgctt gctcaggtgg gaggccggtc cgtccgagcg gacgaattgg
       61 ccgacctgga aacgccggag cccgcggatg aagacggggc cgaggaacaa gaccaaggcc
      121 atcgccgtga ttccggccag ggcggtccgg acggtgatgt agcggaacag gttgaaatcc
      181 ggaaccgacg cctggagggt tttgaaaagg gcgtacagca cgtcagttct ccttcatgag
      241 ggcggtcagc cgctcgacga tccgttccat ccggacgccc cgggatccct tgaccaggac
      301 caggtcgtcg gccttgagca gggccgggag cttcgccgcg gcttcctcgc tcgaggcgaa
      361 ttccggggtc cgggccgcgt ccaggccctc ggcccgggct ccggccgcga tccgccgggc
      421 cgcgggcccg acggccgcga cgatgtccca gcccaagcgc ccggcggtcc gcccggcgtc
      481 ttcgtggtat tgatcccctt ccgggccgag ctcgagcatg tcgccgagaa cagccaccca
      541 ccggccggcc ggaaggttga tatagctgcg gagggccatg tccagggccc gcggattgga
      601 gttgtacgtg tcgtcgatga ccgtgattcc ccgggccagg cggatgatgt ttccccgctt
      661 ggccgccggg gcgaggacgg ccaaagccgg cttcagggtc tcccagccga ggccgaaggc
      721 cgcggcgacc ccgagggcgg ccaggaaatt ttccacctgg ccgccggtca gaaaggggag
      781 agtgacttcg cgggcggccc cgtcgtaatg caggcggaac tggaggccgt catatcccgc
      841 gtaggccatc ccggaagccc ggacttcggc gtcttggctg aagccgaaag tgatgacccg
      901 ccccggggcc cggtcggcga gggtcttgct ccaggggtcg tcggcgttga ggacggccgt
      961 gccgtccggc ttcagtccct caaggatttc cgccttcgcc tcggccacgg cctggagcga
     1021 ttcaaaaaag gccagatgga cgggggcgac gttcgtgacg acggccacgt ccggcggggc
     1081 gatcccggtc agccgccgga tctccccggc cgcgctcatg cccatctcca agacggcgac
     1141 ctcgtgctcc gtctcgaggc ggagcacgga aagggacagg ccgagatggt tgttgaggtt
     1201 tccctcggat ttatggaccc ggaagatcgt ccccagaagg gcggcgatga actccttggt
     1261 cgttgttttg ccgacgctgc cggtgacgcc gacgaccttg gccggatggc ggacgagaac
     1321 cgaccgggcc aggtcctgga gagccgcggc cgtgtccgcg gtcttgatga gaacgaacga
     1381 cttgtcggcg gccgggacgc cgcgggagac gacggccccg gcggctccgc gggcggccgc
     1441 gtccggcacg aagtcatggc cgtccctccc ggcgacgagg gcgaagaaca gcccgccccg
     1501 ctcgatcgtc cgggaatcga ggccgaacga agaaaaaacg gccccccggt cgccctggag
     1561 gaccgttccc ccggcggccg cggcgatttc ggaggcgcgc agaagggcca tggttatttc
     1621 ttctccaaag
//
LOCUS       DAOOXH010000215         1607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_100965_length_1607_cov_6.510309, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000215 DAOOXH010000000
VERSION     DAOOXH010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100965_length_1607_cov_6.510309"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PKV64_12160"
     CDS             <1..853
                     /locus_tag="PKV64_12160"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPN17872.1"
                     /translation="PPVVSGDRIVAAWSNGRVTGLDAAGREVWSVKFSKPIADPLGLA
                     GEKIIVRDPDGFLAALNPADGSTAWKILLPDTAGWTSGGDRIVLRTGDGRLLLFSSEG
                     RPAGEFRVGGKSGGDFSLVDGRILLGFAGGTIGLFDPAKGSLIWRQRLGGVPVGPPAF
                     DGRNIYLVLSNHILAAFHLPSGDILYWKPLAGRAAYRPLLAGSIVFAASRSPRLQAFE
                     AKSGTAKTAYEAGGDVLAAPEILGSRIFLARNGETEQEDILLALVSSPAESPAAPAKS
                     EPQKSIK"
     gene            866..1411
                     /locus_tag="PKV64_12165"
     CDS             866..1411
                     /locus_tag="PKV64_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPN17873.1"
                     /translation="MNREEALGLVTEHLKNKNLVKHCFAVEACMRAMAARLGFDPESW
                     GEAGLIHDLDYEMTGMSAELHTEETAKILESRGVDPAVIHAVRAHAGKVPCQAPMDTA
                     MFAIDPLTGLIVAAALMHPTKKIKNIDVDFVKRRYKEKSFAKGARREDIETCRNLGLE
                     LDEFIGVCLAAMQEIDAALGL"
     gene            1418..>1607
                     /locus_tag="PKV64_12170"
     CDS             1418..>1607
                     /locus_tag="PKV64_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HPN17874.1"
                     /translation="MSVFAVAGRSGAGKTTLLRRLIAELGKRGRTSAVVKHCGGGFDL
                     GGADKDSSAFFWAGARWVT"
BASE COUNT          288 a          517 c          532 g          270 t
ORIGIN      
        1 gccgcccgtc gtctccggcg accggatcgt cgccgcctgg agcaacggcc gggtgaccgg
       61 gctcgacgcg gccggacggg aagtctggag cgtcaaattc agcaagccga tcgccgatcc
      121 cctgggcctc gccggagaaa aaatcatcgt ccgcgatccg gacggattcc tggccgcctt
      181 gaacccggcc gacggatcga cggcttggaa aatcctgctt cccgatacgg ccggctggac
      241 atccggcggc gaccggatcg tcctgcggac cggcgacggc cggctcctcc tgttctcgtc
      301 cgaaggccgc ccggccgggg aatttcgagt cggggggaag tccgggggcg atttcagcct
      361 cgtcgacggg cggattctcc tcggtttcgc cggcgggacg atcggtcttt tcgatccggc
      421 gaagggaagc ttgatatggc gccagcgcct cggcggcgtt ccggtcggtc cgcccgcctt
      481 cgacggccgg aacatctatc ttgtcctctc gaatcacatc ctcgcggctt ttcatctgcc
      541 cagcggcgac atcctgtatt ggaagccgct ggcgggacgg gccgcttatc ggcctctcct
      601 ggctggctcg atcgttttcg ccgcctcgcg gtcgccgcgc ctccaggcct tcgaggcgaa
      661 aagcggaacg gcgaagaccg cgtatgaggc cgggggggac gtcctcgccg ccccggagat
      721 cctgggttcg aggatcttcc tggcccggaa cggggaaacg gaacaagaag acatcctcct
      781 cgcccttgtc tcctcgcccg ccgaatcccc ggccgctccg gctaaaagcg aacctcagaa
      841 atccatcaaa taaaggatga cgaccatgaa ccgtgaggaa gcgctcggcc tcgtcaccga
      901 gcatttgaaa aacaaaaacc tggtgaagca ctgcttcgcc gtggaggcct gcatgcgggc
      961 gatggccgcc cgccttggat tcgatccaga gtcgtgggga gaagccgggc tgatccacga
     1021 cctcgattat gagatgaccg ggatgagcgc cgagctccat acggaggaaa cggcgaaaat
     1081 tctagaaagc cggggcgtcg atccggccgt catccacgcc gtccgggctc acgccggaaa
     1141 ggtcccgtgc caagcgccca tggacacggc gatgttcgcc atcgatccgt tgaccggtct
     1201 catcgtcgcc gccgccctca tgcacccgac gaaaaaaatc aagaacatcg acgttgattt
     1261 cgtcaagcgc cgctacaagg agaaaagctt cgccaagggc gcccggcggg aggacatcga
     1321 gacgtgccgg aaccttggac tggagctcga cgaattcatc ggcgtctgcc tggcggcgat
     1381 gcaggaaatc gacgccgcct tggggttgtg accgccgatg agcgtcttcg ccgtcgcggg
     1441 ccggtcggga gcgggcaaga ccacgctgct tcgccggctg atcgccgaac tggggaagcg
     1501 cggccggacg tcggcggtcg tcaagcactg cggcggggga ttcgatctcg ggggggcgga
     1561 caaggattcg agcgcgtttt tctgggccgg agcccggtgg gtcacct
//
LOCUS       DAOOXH010000216         1604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_101270_length_1604_cov_4.078115, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000216 DAOOXH010000000
VERSION     DAOOXH010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101270_length_1604_cov_4.078115"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1604
                     /locus_tag="PKV64_12175"
     CDS             <1..>1604
                     /locus_tag="PKV64_12175"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPN17875.1"
                     /translation="PYQGGFLIATDKGLSWAGENSIENRWAGVDPLLAGDIRGAAVEA
                     ASGGDVIWLLGSRWLGIFQDGRLRILRRENPLLFSSNFPAAKVLPDGKGGLFFGNPVS
                     IFYYDPSSASAYPLGVRNGLIAEGAAALFLDRESNVWIGGFRGVSRISSRRFHNFRSA
                     QGLLEDEVTAIQAFGDGVVAFGHNFGLTIWDGRELRKIALKAKPDAPPGQIRVLDLAG
                     DDRGGLWLAAASLGLGSLEGNDRIRWRDVPGNPTHPVTSVVFDENRRLWIAQSDALFQ
                     LRKGRPVEVFPGMFKGCYIRKIVAARGGLLFVATTKHGLFRWDGSSWTRSSGDEASGV
                     SNVFSVYLDSNGRTLVGTLAGLRLLAGDRLDLFSENGFEIRRPVYLILEDRAGRLWFG
                     TDNGVVRWDGRVRRDFTKAEGLAGREVNRSAGMIDRLGRIWIGTNTGASCYRENFDLG
                     PDDVPPPTVSITGLDVNGTPADLSAEIVLGYRENNLMFHFQGTSFINEGALRFESYLA
                     GFDKTWGAEYAAADRRIRYTNLPAGS"
BASE COUNT          282 a          507 c          511 g          304 t
ORIGIN      
        1 tcccgtatca gggcggtttt ctcatcgcga cggacaaggg gttgtcctgg gccggggaaa
       61 attccatcga gaaccggtgg gccggggtcg atcctctttt agccggggac atcagaggag
      121 cggccgttga ggccgcctcc ggaggcgacg tcatctggct cctgggttcg cgctggctgg
      181 ggatctttca agacgggcgg ctccgcatcc tcagacggga gaatccgctg ctgttttcct
      241 cgaatttccc ggcagcgaag gttttacccg acggaaaggg cgggctcttt ttcggaaatc
      301 cggtctccat cttctattat gatccgtcca gcgccagcgc ctaccctctg ggagtccgaa
      361 acggcctcat cgccgaaggc gcggcggctc tttttctcga ccgcgaaagc aacgtctgga
      421 tcggcggctt ccggggcgtg agccggatct ccagccgccg cttccataat ttccggagcg
      481 cccagggctt gctggaagac gaagtgacgg ccattcaggc cttcggcgac ggggtggtcg
      541 ccttcggcca taacttcggc ctgaccattt gggacgggcg ggaattgaga aagatcgccc
      601 ttaaagcgaa accggacgct cctccgggcc agatccgcgt cctcgattta gccggagacg
      661 atcggggcgg cctctggttg gcggccgcaa gcctcggctt gggaagcttg gaaggaaacg
      721 accgcatccg ttggcgtgac gtccccggga acccgactca ccccgtcacc tccgtcgtct
      781 tcgatgaaaa ccggcggctt tggatcgccc aaagcgacgc cctgttccaa cttcgcaaag
      841 gccgcccggt cgaggtcttc ccgggaatgt tcaagggctg ctacatccgg aagatcgtcg
      901 ccgcccgggg agggctgctc ttcgtcgcga cgacgaaaca cggcctcttc cgctgggacg
      961 gaagctcctg gacgcggtcc tcgggagatg aggcttccgg cgtgtccaac gtcttttcgg
     1021 tctatcttga ttccaacggg cgaaccctgg tcggaacgct ggccggcctg cgcctgctcg
     1081 ccggcgaccg gttggacctt ttctcggaaa acggattcga gatccgccgc cccgtgtatc
     1141 tcatcctcga agaccgggcc ggccgcctat ggttcggaac ggacaacggc gtcgtccgtt
     1201 gggacggccg ggtgcgccgc gatttcacga aagccgaggg gctcgccgga cgggaagtca
     1261 accggtcggc cgggatgatc gaccgcctgg gccggatctg gatcgggacg aacacagggg
     1321 cctcatgcta ccgagagaat ttcgacctgg ggccggacga cgtccccccg ccgacagtct
     1381 ccatcaccgg cctggacgtc aacgggacgc cggccgattt atccgcggaa atcgtccttg
     1441 gataccggga aaacaacctc atgttccatt tccaagggac ctccttcatc aacgagggcg
     1501 ccctccggtt cgaaagctac ttggccgggt ttgataaaac atggggagcc gaatacgcgg
     1561 ccgccgaccg gcggatccgc tacacgaatc ttccggcggg atcc
//
LOCUS       DAOOXH010000217         1602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_101455_length_1602_cov_5.279897, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000217 DAOOXH010000000
VERSION     DAOOXH010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101455_length_1602_cov_5.279897"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..427)
                     /locus_tag="PKV64_12180"
     CDS             complement(227..427)
                     /locus_tag="PKV64_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17876.1"
                     /translation="MALERETALMLMDLWAFAYTQNPSRKTKNEFESACRLGDALSLP
                     EVNVFRELVSGLEQELVELQAD"
     gene            567..1079
                     /locus_tag="PKV64_12185"
     CDS             567..1079
                     /locus_tag="PKV64_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17877.1"
                     /translation="MSAFSLRTRKRLAAALVFMIAGPAGTDVVFVLEQEVSDLFRRIY
                     EEVQTMPARPGERFIRQEFFIGEGDDDTYKDIAVSIVLPGEDAPPDMIVQITWMKRVR
                     RDRRVAVARSTKTLRFILEADGTRLEGDEPANGEIAVLARGLFTAVRNKKRLLGKAPS
                     RGFREPREYP"
BASE COUNT          301 a          462 c          495 g          344 t
ORIGIN      
        1 tgggcaaggt ccaccgtctc aatctcatcg atcccctgac gggggaggag atcaaataaa
       61 gggcggtcct tctgattgat ccgtcgattt catcgtcccg gggccccgaa gggccccggg
      121 atttttttgg gaagaggaaa actcggcctc gaggccgagg aggaagcatc gccggattta
      181 ttcgcggcgt ttgatcttgc ggccctcagc ccgagttcga acagcctcag tcggcttgga
      241 gctcaaccaa ttcctgctcg agcccggaga cgagctcccg gaagacattc acctcgggta
      301 aagatagggc gtcgccgagg cggcaagcgc tctcgaattc attcttcgtc ttccgggagg
      361 ggttctgggt ataggcaaaa gcccagagat ccataagcat caaagccgtt tccctctcaa
      421 gcgccatttg atttctcctt gtcccttttc atacctgcaa ggaatgtgcc aaggagcgcc
      481 ggcccgggaa gctgttttaa aagtccggat tcattgacgc gacgcttttt ccggtttacc
      541 cttatctcaa agggcaagaa cggctcatgt cggctttttc gcttcggaca cgaaaacgac
      601 tggcggcggc cctggtcttt atgatcgcgg ggccggccgg aacggatgtc gttttcgttc
      661 tggaacaaga ggtttcggat ctcttccgcc ggatttacga ggaagtccag accatgccgg
      721 cccgtcccgg cgaacgattc atccgccagg aattcttcat cggggagggc gacgacgaca
      781 cgtataagga tatcgccgtc tcgatcgtcc ttccggggga agacgctccg ccggacatga
      841 tcgtccagat cacttggatg aagcgcgtcc gccgcgaccg gcgggtggcc gtggcccggt
      901 cgacgaaaac cttgcggttt attctcgagg cggacggcac ccggctggaa ggggacgagc
      961 cggcaaacgg cgagatcgcc gtcctggccc gcggtttgtt cacggctgtc cggaacaaaa
     1021 aacgtcttct ggggaaggcg ccgtcccggg gcttccggga gccccgggaa tatccataac
     1081 gggctttccg ggagcatctc ccggattccc tgatgtaaaa ttcgtttact aatcattaaa
     1141 gattgttttt tttccgggtc gacgcgggtt ttcttccaaa aggactctga cgatcttcca
     1201 ggccccgcct ttggccttgg ccggggtgaa aaaaatcgat gaaccgggag ttgtcgctcc
     1261 tgacttaggc cgtcgtttcg gtcatgtcga ggatcgtgat ctcgatgcgg cgccgatcag
     1321 cggaacgcgg attccgccgc ctcgctcggt gtattcgagg atgatgtcct tggtcggctc
     1381 ggccgccttt tcccgtccgg tccggagatc gacttcaaca agcccttggc cggccgggga
     1441 tgaagacccc ggtccatgcg ttcgacgcgg ccttcgaatc agccttcgag atcgtcgagg
     1501 gcggcccggt ggacggccga ttcttcggct tcccgggggg aggagggcaa cgccgcggcc
     1561 gacccggcca cggcggccgg gaggagaacg atcattcggg ga
//
LOCUS       DAOOXH010000218         1588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_102807_length_1588_cov_6.010437, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000218 DAOOXH010000000
VERSION     DAOOXH010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102807_length_1588_cov_6.010437"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1588
                     /locus_tag="PKV64_12190"
     CDS             <1..>1588
                     /locus_tag="PKV64_12190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPN17878.1"
                     /translation="TMMRRSAAARPVIVMLAACLFLVPAGPARPSGSELSPAHRKWLE
                     EDVDGILSSRERDVFLRLESDRQRDLFIAAFWKRRDPVPATPENEFKAEHERRLAYAD
                     AHFGRTAARPGRKTGRGRMYVLLGEPDDIVRYSGKDGLREAEVWFYRNKENAGSAGLP
                     PSFNLVFFQEGSVGDFKIYSPFQDGPAALLSDFRGDPGDYEKAYRALSALDAGLAAVS
                     FSLIPGEASGFPGRPSPSSDILLQRIAASGAGRIDDEYAAKFLEYKDIVEVEYSANYL
                     DSRGTMIVRREPSGTAFVHYALEPARLSLAAAGREYAATLRLNGAVSLEDGRVISTFE
                     KIVSVRMDEERKNTLSRVPFMIQDLFPLIPGIFKVSLLLRNEASKEFCAFERTVVVPE
                     SGSGPGLTAPLLGYRARSSDVEPSNLKPFRFGSTQVYFQSGRTLARSDDLVLAFQVLG
                     LDGPVRSRARLRFSITRDDVAIEEWERDLSGFSGVSDIVESRPASGLSPAFYAARVRL
                     FIDGREAAAGGGDFAVTPLEA"
BASE COUNT          243 a          542 c          488 g          315 t
ORIGIN      
        1 actatgatgc gccgctcggc cgccgcccgc ccggtgatcg tgatgctggc ggcttgtctt
       61 ttcctcgtcc cggccgggcc cgcccggccg tccggatcgg agctgtcccc cgcccaccgg
      121 aaatggctcg aggaggatgt cgacggcatc ctatcgtccc gcgagcggga cgttttcctc
      181 cggctcgaat ccgaccggca gcgggacctt ttcatcgccg ccttctggaa gcgccgcgat
      241 cccgttccgg cgaccccgga aaacgaattc aaggcggagc atgagcgccg gctcgcttac
      301 gccgacgcgc atttcggccg cacggcggcc cggcccggcc ggaaaaccgg ccgcgggcgc
      361 atgtacgtcc tcctcggcga gccggacgac atcgtccgat attccggcaa ggacggcctc
      421 cgcgaggccg aggtctggtt ctaccggaac aaggagaacg cgggatcggc gggcttgccg
      481 ccgtcgttca acttggtctt ttttcaagaa gggtctgtcg gcgacttcaa gatctacagc
      541 ccgttccagg acggaccggc ggctctcctc tccgatttcc ggggggatcc cggggattat
      601 gagaaggctt atcgggcctt gtccgctctc gacgcgggct tggccgcggt ctccttttcc
      661 ctgattcccg gcgaagcgtc cggttttccg gggcgtccgt ccccgtcctc cgacattctt
      721 ctccaaagaa tcgcggcttc cggcgccggc cggatcgacg atgaatacgc ggcaaagttc
      781 ctcgaataca aggatatcgt cgaggtcgaa tacagcgcca attacctgga cagccggggg
      841 acgatgatcg tccggcgcga gccttccgga accgcctttg tccattacgc cctcgagccg
      901 gcccgtcttt ccctcgctgc cgccggccgg gaatacgccg cgacgctccg cctcaacgga
      961 gccgtttcgc tggaggacgg ccgggttatt tccaccttcg aaaaaatcgt ttccgtccgg
     1021 atggacgaag agcggaaaaa cacgctgagc cgcgtgccgt tcatgatcca ggatctcttc
     1081 cccttgatcc cgggaatctt caaggtctcc ctgctgctca ggaatgaggc ttccaaggaa
     1141 ttttgcgcgt tcgagcggac ggtggtcgtc cccgaatccg gctccggacc cggtctcacc
     1201 gccccgcttc tcggctatcg ggcccgatca tcggacgtgg aaccctcgaa tctcaagccg
     1261 ttccggttcg gctcaaccca ggtttatttt caatccggcc gaaccctcgc ccgttcggac
     1321 gacctggtcc tggctttcca agttctcggc ctcgacggcc cggtcagaag ccgggcccgc
     1381 cttcggttct ccatcacccg ggacgacgtt gcaatcgagg aatgggagcg ggatctttcc
     1441 ggcttttccg gcgtttcgga cattgttgaa tcgaggccgg cctccggctt gagcccggcc
     1501 ttctacgccg cccgggtccg gcttttcatc gacgggcggg aagcggccgc cgggggcggg
     1561 gatttcgccg tcacgccgct ggaagcgg
//
LOCUS       DAOOXH010000219         1584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_103209_length_1584_cov_6.986266, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000219 DAOOXH010000000
VERSION     DAOOXH010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103209_length_1584_cov_6.986266"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PKV64_12195"
     CDS             <1..794
                     /locus_tag="PKV64_12195"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPN17879.1"
                     /translation="YFRRHGGPPMTILVIARHTFKEAVRDRILYLLLFFSSLSIILAR
                     VLAVLTVGDRVKVVKDVCLASIAFFGALMAILIGTGLVYKEIEKRTIFTLISKPLRRS
                     EFLLGKYLGLVLTLAAMVAAMTVISLAVVFLHTFTLEIQMLPAVFFIFLELMLLTAVA
                     VLFSCFSTPILSSLFALSFYLIGHFSWGLDTLIRKTPPGIGRTLLRVLDAILPDLENF
                     NIKTEVVQNLPLPSGFGLGAAGYAVVYTAFVLLLAVLIFRRRDFV"
     gene            798..>1584
                     /locus_tag="PKV64_12200"
     CDS             798..>1584
                     /locus_tag="PKV64_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17880.1"
                     /translation="MAGKTPLAPAALLLLLGGLAGIKVGLDGMPRAKIPGAGIVYLPS
                     GPAVKAAVFGFSSLTADVSYVWAIQYYGNTAVPDRRRHFNRIFEVIADLDPNWMDPYL
                     TAALIAHHDFRDPELALKMYDDGAARNPGAWIFPFEAGHYCQLYLRDYERAKSYYRKA
                     MDLPEAPAIARRLFANAAFKTMDYQTSWETWREVYETAPDREIKKIASNHLYNIKASV
                     DIAALNAALERFREIRGRAARRLDELASTRLIAAVPKDFDGKDY"
BASE COUNT          256 a          551 c          438 g          339 t
ORIGIN      
        1 gatattttcg tcgacacggt ggcccgccga tgacgatcct ggtcatcgcc cgccacacgt
       61 tcaaggaggc cgtccgcgac cggatcctct acctccttct ctttttctca tccctgtcga
      121 tcatcctggc ccgggtcctg gccgtcctga ccgtgggcga ccgggtcaaa gtcgtcaagg
      181 acgtctgctt ggcttcgatc gccttcttcg gggctttgat ggccatcctc atcgggacgg
      241 ggctggttta caaggagatt gaaaagcgga cgatcttcac tctgatttcc aagcccctcc
      301 gccggtcgga gttcctgttg ggaaaatatc tcggtctcgt cctgaccctg gcggccatgg
      361 tcgcggccat gaccgtgatc tcgctggccg tcgtcttcct ccacacgttc actcttgaaa
      421 tccagatgtt gccggcggtt ttcttcatct ttctggagct gatgcttctg acggccgtgg
      481 ccgttctctt ttcctgtttc tccacgccga tcctcagctc gctgttcgcc ctcagcttct
      541 acctgatcgg ccatttctcc tgggggctgg acaccctgat ccggaaaacc cctccgggga
      601 tcggccggac cctgctccgg gtcttggacg cgatccttcc cgacctggag aatttcaaca
      661 tcaagacgga ggtcgtccag aacctgccgc ttccctccgg cttcggactc ggcgcggccg
      721 gctacgcggt cgtctacacg gccttcgtcc tgctgctggc cgtcttgatt ttccggcggc
      781 gggatttcgt ctgagccatg gccggaaaaa caccgcttgc cccggccgcc ctgctcctcc
      841 tgctgggcgg cctcgccggt atcaaagtcg ggcttgacgg gatgccccgg gcgaagatcc
      901 ccggggccgg catcgtctac ctgccgtccg gaccggccgt gaaggccgcc gttttcggct
      961 tttcctcgct gacggccgac gtttcctatg tctgggcgat tcagtactac ggcaacacgg
     1021 ccgtcccgga ccgccgccgc cacttcaacc gcatcttcga ggtcatcgcc gacctcgacc
     1081 cgaactggat ggatccatat ctcacagcgg ctttgatcgc ccatcacgac ttccgggacc
     1141 cggagctcgc cctgaagatg tacgacgacg gcgcggcccg caatcccggg gcctggatct
     1201 tccccttcga agccggccat tactgccagc tttatctccg ggactacgag cgggccaaaa
     1261 gctactaccg gaaggcgatg gacctccccg aggcgccggc catcgcccgg cgtctcttcg
     1321 ccaatgccgc cttcaagaca atggactacc agacgtcctg ggagacctgg cgcgaggtct
     1381 acgaaaccgc gcctgaccgc gaaatcaaga aaatcgccag taatcacttg tacaacatca
     1441 aggcgtccgt cgacatcgcc gccttgaacg ccgccctgga acggttccgg gaaatccgcg
     1501 gacgcgccgc ccgacgactc gacgagctcg cgtccacccg cctgatcgcg gccgtcccga
     1561 aggatttcga cgggaaagat tact
//
LOCUS       DAOOXH010000220         1540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_107680_length_1540_cov_4.562290, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000220 DAOOXH010000000
VERSION     DAOOXH010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107680_length_1540_cov_4.562290"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PKV64_12205"
     CDS             <1..207
                     /locus_tag="PKV64_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPN17881.1"
                     /translation="IAFFDSAVAWDKEHKAWFLGGDRKPLSSYGIGLRANVFGYFIVG
                     CDYVKPLDRPGQGWHFAFSFWQGF"
     gene            263..1387
                     /locus_tag="PKV64_12210"
     CDS             263..1387
                     /locus_tag="PKV64_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018754474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HPN17882.1"
                     /translation="MRKTLLSRAGSAAGLLLAMSLALAAERPRIPEPRISFDPRTYVC
                     YRAAGPVTVDGNLDEAVWEKAPWTEPFVDIEGGRRPKPRFRTMAKMLWDDRYFYIGAY
                     LEEPNVWATLAAHDSIIYQENDFEVFIDPDGDTHDYYELEINALNTVWDLFLVNPYRD
                     GRPANIHSWEIPGLQTGVLVNGTLNNPADKDRSWFVEMALPWAVLKEAAQPAAAPKPG
                     DQWRVNFSRVEYRTTTTGGTIVKAVDPATGNPYPEDNWVWSPMGLVNIHYPELWGFVQ
                     FSEKTAGKGKEAFVARPEEKAKWALRRIYYREWAYFAEKGVFTADLKALGLTERELKV
                     SGFVSPPVLQATERLFEASFTRPDGAVWRIGPDGRVRKDS"
BASE COUNT          279 a          471 c          486 g          304 t
ORIGIN      
        1 atcgcctttt tcgacagcgc cgtggcctgg gacaaggagc ataaagcctg gttcctcggc
       61 ggcgaccgca aacccctgtc cagctacggg atcggcctgc gggcgaacgt cttcgggtat
      121 ttcatcgtcg gctgcgacta cgtcaagccg cttgaccgtc ccggccaagg ctggcatttc
      181 gctttcagct tctggcaggg attttaaggc ttcggaatcg acgcatttct gctaaaatgg
      241 cttcttcccg atggaggagg ccatgcgaaa aacgctttta tccagggccg gctcggccgc
      301 cggactcctg ctcgcgatgt ctttggcgct cgcggccgaa aggccgcgga ttcccgaacc
      361 gaggatttcc tttgatcccc ggacctatgt ctgttaccgg gcggccggcc cggtgacggt
      421 ggacggaaac ctggacgaag cggtctggga gaaagctccc tggaccgagc ccttcgtcga
      481 catcgagggc ggccggcgtc cgaagccgcg gtttcggacg atggccaaga tgttatggga
      541 cgaccggtat ttttatatcg gggcttacct ggaggaaccg aacgtctggg cgacgctcgc
      601 cgcccacgac tcgatcatct accaggagaa tgatttcgag gtcttcatcg atcccgacgg
      661 cgacacccac gattactacg agctggagat caacgccctc aacaccgttt gggacctgtt
      721 tcttgtcaat ccctaccggg acggccggcc ggccaacatc cattcctggg agatccccgg
      781 cttgcagacc ggcgtcctgg tcaacggaac gttgaacaat ccggcggaca aggaccggag
      841 ctggttcgtc gagatggccc ttccctgggc cgtgctcaag gaagccgccc agccggcggc
      901 cgcgcctaag ccgggcgacc agtggcgggt caatttctcc cgggtggaat accggacgac
      961 gacaaccggc ggaacaatcg tcaaggccgt cgatccggcc accgggaatc cttatcccga
     1021 ggacaactgg gtttggtcgc cgatggggct ggtcaacatt cactatcccg aattgtgggg
     1081 attcgtccag ttttcggaaa aaaccgccgg caaggggaag gaggccttcg tcgcccggcc
     1141 cgaggaaaaa gccaagtggg ccctgcgccg aatctattac cgggaatggg cttacttcgc
     1201 cgagaaaggc gtgttcacgg cggacctgaa ggctctcggc ttgaccgaaa gggagctcaa
     1261 ggtttcgggt ttcgtttctc cgcctgttct ccaggcgacc gagcggcttt tcgaggcgtc
     1321 cttcaccagg ccggacggcg cggtctggcg catcgggccg gacgggcgcg tccggaagga
     1381 ttcatgaaat tcgattttga cgccgtcctg gaccggcggg ggaccggttc tttgaaatgg
     1441 gacttcccgg tcctgaagct gaacgccccc ggggctcttc ccctgtgggt ggccgacatg
     1501 gatttcgccg ccccgccggc cgtttgtgaa gccgtcgccc
//
LOCUS       DAOOXH010000221         1516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Aminicenantota bacterium isolate
            AMR_MDS_3594 NODE_110249_length_1516_cov_7.325120, whole genome
            shotgun sequence.
ACCESSION   DAOOXH010000221 DAOOXH010000000
VERSION     DAOOXH010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865329
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Aminicenantota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Aminicenantota bacterium
            Bacteria; Candidatus Aminicenantota.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:38:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,443
            CDSs (total)                      :: 2,401
            Genes (coding)                    :: 2,389
            CDSs (with protein)               :: 2,389
            Genes (RNA)                       :: 42
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Candidatus Aminicenantota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110249_length_1516_cov_7.325120"
                     /isolate="AMR_MDS_3594"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052149"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..>1516)
                     /locus_tag="PKV64_12215"
     CDS             complement(89..>1516)
                     /locus_tag="PKV64_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPN17883.1"
                     /translation="PAAARGGLLHRYDFKSRKDEVLLTGVFQYTLSFDGKKILYSGPS
                     ETFFITALQPKPAAGQGQLQVGEIGVQVVPEEEWAQIYAEAWRINRDYFYADNMHGAD
                     WEAMRKKYEVFLPHLSCRADLNRLIQWMCSELGVGHHRVGGGDFLAANRAVPGGLLGA
                     DYAVENGRYRIKKIYGGLNWNPDLRSPLTEPGLNVAEGDYLLAVDGRDLRAPADNLYA
                     LFENKAGKFVELKVGPLPDGRGARTVRAVPVENELALRNRDWVEGNLKKVEKATGGRV
                     AYVYVPNTSYQGFAYFKRYFYPQADKEAVIVDERFNGGGLIADYYIDHLRRPEISYWN
                     MRHGADMKAPGASIQGPKVMLIDETAGSGGDLLPWMFRKFKIGPLVGKRTWGGLVGTL
                     GFPVLMDGGYVTAPNLAIWTEDGWIVENEGVPPDVEVEQDPAAVAAGGDPQLERAIAI
                     VMEELAKSPAKKPVRPPYPIRGNRK"
BASE COUNT          253 a          513 c          464 g          286 t
ORIGIN      
        1 aaccccgcga tattttctcc cgccgccgcg tcttgggatt tcggcggagc ggggctgaaa
       61 tccagccccg ctccgcctga tttccatttt acttccggtt gccgcggatc ggatacggcg
      121 gccggaccgg cttcttggcc ggcgatttgg ccagctcctc catcacgatg gcgatcgccc
      181 gctcgagctg cggatcgccg ccggcggcga ccgcggccgg atcctgttca acctcgacat
      241 ccggcggcac gccctcgttt tcgacgatcc agccgtcctc cgtccagatg gccaggttgg
      301 gcgcggtgac atagccgccg tccatcagga cggggaagcc gagcgtcccg accaggccgc
      361 cccaggtgcg tttcccgacc agcgggccga ttttaaactt gcggaacatc cacggcagga
      421 ggtctccccc cgacccggcg gtttcatcga tcaacatgac cttggggcct tggatcgaag
      481 cccccggcgc tttcatgtcg gcgccgtgcc gcatgttcca atagctgatc tccggccggc
      541 gcagatgatc gatgtaataa tcggcgatca agccgccgcc gttgaaccgc tcatcgacga
      601 tgaccgcctc cttgtcggcc tggggataga aatacctctt gaaataagcg aatccctggt
      661 acgacgtgtt ggggacatag acataggcga cccggccgcc ggtggctttc tccaccttct
      721 tcaggttgcc ttccacccag tcgcggttgc ggagggccag ctcgttttcg accggcaccg
      781 cccggacggt ccgggctccc cggccgtcgg gaagcggccc gaccttcagc tcgacgaact
      841 tgccggcctt gttctcgaac aaggcgtaaa ggttatccgc cggagcccgc agatcccggc
      901 cgtcgaccgc cagcaggtaa tccccctcgg ccacgttcag gccgggctcg gtcaggggcg
      961 agcgcaggtc gggattccag ttcaatcccc cgtagatttt cttgatccgg taccggccgt
     1021 tttcaacggc gtaatccgcc ccgaggagtc cgcccggaac ggcccggttc gcggccaaaa
     1081 aatccccgcc gccgacccgg tggtggccga cgccgagctc ggagcacatc cactggatca
     1141 gccggttgag atcggcccgg caggacaagt gcggcaaaaa cacttcgtat tttttccgca
     1201 tggcctccca gtcggccccg tgcatgttgt cggcatagaa gtaatcccgg ttgatcctcc
     1261 aggcctcggc gtagatctgg gcccattcct cctccggaac gacctggacg ccgatctccc
     1321 cgacctgcag ctgtccctgg ccggcggccg gcttgggctg gagggcggtg atgaagaagg
     1381 tctccgacgg tccggagtac aggattttct tgccgtcgaa ggaaagcgtg tactgaaaga
     1441 cgccggtcaa gaggacctcg tccttgcggg atttgaaatc ataccggtga agcaggccgc
     1501 cgcgcgcggc cgcggg
//
